LOCUS       DAOSMF010000001       140070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_103_length_140070_cov_21.260308, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000001 DAOSMF010000000
VERSION     DAOSMF010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 140070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 140070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..140070
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103_length_140070_cov_21.260308"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..240
                     /locus_tag="PLT63_00005"
     CDS             94..240
                     /locus_tag="PLT63_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97132.1"
                     /translation="MKRKDLPLIKTNPYLQDPSECDAWLNRAVISSSAVEGARAAACR
                     ALGV"
     gene            complement(515..946)
                     /locus_tag="PLT63_00010"
     CDS             complement(515..946)
                     /locus_tag="PLT63_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97133.1"
                     /translation="MPQSAKGMGRLTVLTDQARALRLRRINRIRTIRGSLAIEGNTLN
                     EAQITALLEGKRVIAPPREVQEVKNALAAYDRFGVWKPETEKHLLEAHRLMMSGLLDE
                     AGRYRPPSHPPSRRTAGGHPGRDGPGGAAIRSAPFRPQIVP"
     gene            complement(1176..1505)
                     /locus_tag="PLT63_00015"
                     /pseudo
     CDS             complement(1176..1505)
                     /locus_tag="PLT63_00015"
                     /inference="COORDINATES: protein motif:HMM:NF022970.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
     gene            complement(1629..2852)
                     /locus_tag="PLT63_00020"
     CDS             complement(1629..2852)
                     /locus_tag="PLT63_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97134.1"
                     /translation="MILRLTAKLAQKIGLDPLPALPYDKGRNPLLDWHAHLFTVQRTH
                     YILATNTASLYSLVMLGRGITMGRQFAQALSDGLQDFLTGQGYQPNAVMTLLSPVQDA
                     IFAKTTDRRVLGSMNDLIFQSKIRLGEGQQTPFAVSYYLNETPMSYLKYRSPKDLFQE
                     LFRQGGKPSDQGQKPNNVIYLEKFKPVAQDNGPVLADYDRLQSELIRDKVREIFRAHP
                     RDPVAEMEKLGFTYCEDDTEDEAKEERNARPENRRQRDLVAYFEGRKPLSEEIFAGYS
                     EEKAAEKPNYPLIRRYYKASNKHLKALLLYGLEHHPGRIDLLSDLAFFHEFENCLRLL
                     IDHYTQACMEQCNLDTFTGLAKDFYYSTSPNGYEAYEALRELFGPETAKRKIIDSLIA
                     EEEGGAEDSSRPLDS"
     gene            complement(2937..3152)
                     /locus_tag="PLT63_00025"
     CDS             complement(2937..3152)
                     /locus_tag="PLT63_00025"
                     /inference="COORDINATES: protein motif:HMM:NF023295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HOD97135.1"
                     /translation="MLAALKAGKLVLDWRKRQQARAEVRVTIEKLLDKGLPRAYTPEL
                     FEQKTAAVFQHVYDAYYGAGRSIYAAA"
     gene            complement(3193..3528)
                     /locus_tag="PLT63_00030"
     CDS             complement(3193..3528)
                     /locus_tag="PLT63_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97136.1"
                     /translation="MVPENRIQDGTFPIPKLFVGKEDIDQFTDEFRGFHAQFADCFSR
                     EEPRENFVQYMAGQMSQLEWRTARLGFLPPMVGIPLIPPLASEGSRHPVPMQSARGFR
                     FIPPPLVGA"
     gene            3742..4560
                     /locus_tag="PLT63_00035"
     CDS             3742..4560
                     /locus_tag="PLT63_00035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOD97137.1"
                     /translation="MATKYIKGKLYKLKIADLQPDPQQARTFMDTVALNELAASIEKY
                     GILVPIQFCLNEEGGLMIVSGHRRVKAAAKAGLTEIHGTFTEGDIRLQGFVDNLQREG
                     LTPVDEAEQMKLMMKEYAFNQYQLADALGKDRTTVTKTLSINKLPEDIRSAARTNPNI
                     PKSVLLDAARMSTEKSMRRKFLSYMTQAAKAGQTSARKQRLSKERILITKTDNLTGEL
                     DGLPWREWAEDDRNDLASALAGIRRKADELLAEMEWNREEEEEEKPSASGMNLS"
     gene            5110..5622
                     /locus_tag="PLT63_00040"
     CDS             5110..5622
                     /locus_tag="PLT63_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001201532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HOD97138.1"
                     /translation="MKYISIIMAFLLMGCVGIPENVKPVENFKSEKYLGRWYEIARLD
                     HSFERGLTRVAADYSLLANGGVRVINRGYSIKDKTWKEIEGKAYFVERSDQGYLKVSF
                     FCPFYGSYIVFELDHEHYQYALVCGPDKSYLWILARAPEINRDTLAVLTAKAAKLGFD
                     TGKLIFVDHR"
     gene            complement(5660..7381)
                     /locus_tag="PLT63_00045"
     CDS             complement(5660..7381)
                     /locus_tag="PLT63_00045"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOD97139.1"
                     /translation="MDELSILMTFAVGLAGALVFGYIATRLKLSPIIGYLIAGVMVGP
                     FTPGVVVNRVIAEQFAEIGVILLLFGIGLRFNLKELFAVWRLAIPGALIQSTLSTLAL
                     ASILHLMGWSWISGLILGMAISVASTVVMALVLAKWHDLHATIGHVAIGWTVVEDILT
                     VALLLLLPLLFSQNPNAELNIGWVLGLAGIKILGLLVLVVVLGKWAIPRALESIAKTR
                     SRELFTLAVLVLAVGIAVGAAKVFGVSMELGAFLAGLAVGRSEFAARAANDALPMRDA
                     FAVLFFIAVGMLFDPKSLIQVPHIIAAVLFVVIIVKPAAAAITVRMLGQPAATAIPIG
                     AAFSQVGEFSFILGTVALGLGLLDESGWNALVATSIISIGFNPYIYDWARRFSTAKKT
                     KTTPVVEIPRQPLNPDLCILVGYGPVGKIVHRLLLDRGAEITVIDLNLDTIRQLRAEG
                     HNALYGDVLRSGTLEDAGIAVATSLILSTDIDDAAEVVKHARQVNPDLKILVRCAHLR
                     EAGSLRKAGATVVVAGEAEVGVALAEVVTAGDEMACNLAADHRETIRRMLYDTPMHLE
                     EKSRSKS"
     gene            7559..8377
                     /locus_tag="PLT63_00050"
     CDS             7559..8377
                     /locus_tag="PLT63_00050"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOD97140.1"
                     /translation="MKSRFIVLIDFSPNSEQLLRFAHDWSKRVGAELLAFHNTIALSP
                     LMTPYETKTTLIDRANYEARERLVKFTENVLPPATSIQHLVSEENMVAVLRELLKKPF
                     SHLVFLGIKGTGLLKKIFIGSQAVNVINGIDNLIVAMPQHAPCCAPDAIHVAVQKTYP
                     LNLVELNEIIRLNGEGGSKIIFFSVVTSEDDREATERYLKALTQLYSDKKETAYELYL
                     GDNAWRDLKKIIKQKQKEFIVVQRGSRMFLDQITRKFLINDLVYEGHTPLIIMP"
     gene            complement(8508..8873)
                     /locus_tag="PLT63_00055"
     CDS             complement(8508..8873)
                     /locus_tag="PLT63_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97141.1"
                     /translation="MDAATDQKPEKVRINQNNLYREEIFTDMTVGSLHQMTPVKANGE
                     ADKQRKVLFIGNTQLITKQGPLPIRFPIEAKNLPQAAEKFPEAMESFVAMMIAQAKEM
                     QRQEESRIIVPGSGNIKLA"
     gene            9361..10215
                     /locus_tag="PLT63_00060"
     CDS             9361..10215
                     /locus_tag="PLT63_00060"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOD97142.1"
                     /translation="MWKKEQIRHVIKRMVAPVTIMFIPHHHAKHSFNLNIPFLFIPLI
                     SILTILGFIYAGSMVGDLVRYQVMERQMRDYTAKVGEFNAALSAVKQMEQELKTLMSQ
                     GSKGKILENYESADMGTVDMYQIQKQIEISMETVAGIRDYLRNQKDLYMATPRGFPIS
                     GKITSLFGSRANPITGRVELHRGIDIAAPAGTTVQATADGVVSFSGWNRGGGNTVVVE
                     HGHGFSTCYAHNRANVVRVGQRIKRGDVVGYVGATGNATGNHLHYEIWQDGRVINPRQ
                     SLEVARVP"
     gene            10205..10603
                     /locus_tag="PLT63_00065"
     CDS             10205..10603
                     /locus_tag="PLT63_00065"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOD97143.1"
                     /translation="MFHRRADRLKMVLGENARITGDVESTGAVFIDGAIRGNVEGAKV
                     ILGEHGCVDGDIKAGTIIIGGTVTGRLDGAEKVEIKATGRVAGDIETKVLVIAAGGVF
                     NGTVQMEGAGNSSGECGDGKVVEFLVKEQP"
     gene            10610..11248
                     /locus_tag="PLT63_00070"
     CDS             10610..11248
                     /locus_tag="PLT63_00070"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOD97144.1"
                     /translation="MKIYAVADIHGRKSRLERIRSAAAAARPDLVVIAGDITGFGNQD
                     VFLAGLDSLNLPILAVPGNTDSAELEKDLEKYPNILSIQLRKEVIDGVSFIGLGGTLS
                     VPFLEKLRPKDNRGLDALAEDLGDEDILVTHTPPWGILDRGFASLHGGSRWLRELLER
                     RHPRLLICGHIHEDRGAATVERTTVVNCSMGRKGAGALIKAKKGGGMEIAFL"
     gene            complement(11262..12188)
                     /locus_tag="PLT63_00075"
     CDS             complement(11262..12188)
                     /locus_tag="PLT63_00075"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOD97145.1"
                     /translation="MTDSAAPPIFPTIMGCEEIHFGPVWFIPGVNRGRYPYCHSLYIQ
                     GPGIIIDPASDEKRLLEIRDLEGVNEVWLSHWHEDHITYLDLFPGAAVRICRRDEPPL
                     GDLEILLDWYNLSEPHRDHWRQVLTETFRYRPRRADAYLTEGDVHSFATTTVQVIGAP
                     GHTPGHLAFYFPEQEVLFVGDYDMTSFGPWYGDRNSSIEATISSLRRLREIPARTWIT
                     GHDTGVFTDNADERWRRYEDIIHQREANLLNLLARPHNLQEIVAAWLIYKKPREPLAF
                     FEFGERALNVKHLDRLMEKGLVVELNGRYVQA"
     gene            12413..13060
                     /locus_tag="PLT63_00080"
     CDS             12413..13060
                     /locus_tag="PLT63_00080"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOD97146.1"
                     /translation="MKIILLGAPGAGKGTVAKLLSEYDGSVQISTGDILRSAVKTGSE
                     LGKKAKAYMDAGDLVPDELIMALMRERLAAPDCRQGFILDGFPRTIPQADALKGLLRE
                     LRIELDFVVSLEVPREVILDRLTTRRTCSNPDCQEIYNIKSNPPLPDGRCKKCGSLTI
                     QRDDETEAAIQYRLETYEKKTSPLIEFYRTEGLLRQFPSVSSVETVAAVKRQLGK"
     gene            13057..15024
                     /locus_tag="PLT63_00085"
     CDS             13057..15024
                     /locus_tag="PLT63_00085"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HOD97147.1"
                     /translation="MIDYDGELNAEQRRVVLEPGGPLLVIAGAGSGKTRTLTYRVARL
                     IESGCPPGRIVLATFTNKAARSMMSRAADLMSTEISRLWGGTFHHLAHRVLRAHAHRL
                     DYENNFVIIDADDAKQLVNRCLTEKLKNLTPKFPRSDVLRDIISFARNTDRRLEEVIA
                     DRFPYLESCLEGIMTVARHYADEKKRLNVMDFDDLLINWRLLLADHEDILADYSERFR
                     HILVDEYQDTNIIQADITDLLASRHRNIMVVGDDAQSIYAFRGANYANIMKFPDKYLD
                     CKIFKLEMNYRSTPEILHMANLSIRNNERQFEKSLRAVRPRGIMPVLASPRNVQMQAE
                     FVAQRIIELIGEGYSPGEIAILYRAHFHSLELQMELTRRGIPFTIRSGIRFFEQAHIK
                     DVTAYMRIVVNPGDEMAWRRVLSLCHRIGRQTAGKIWKWLANNREPLAIFLSDAALGK
                     VGAAARPGLAGLQELLAALKDVEGGDMGTIIETILRRGYESYLQSTYPDAVQRLEDLG
                     QLAVFATRFSTPGEFLQEMALLTGIGEEGEGLADADAGEGRVIMSTIHQAKGLEWSVV
                     FIPWCAEGMIPLGRALADPEGEEEERRLFYVAVTRAKDQLYLCYPAVEYRRGMGYVAV
                     KSSRFISELTPTVSPSRRGRPYERWLVEEDY"
     gene            complement(15031..16920)
                     /locus_tag="PLT63_00090"
     CDS             complement(15031..16920)
                     /locus_tag="PLT63_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone-lysine N-methyltransferase"
                     /protein_id="HOD97148.1"
                     /translation="MAKWNKNKKILDHEHTKFWRYELKDIAEPNLQKDVFPYEQVSRI
                     DFDHKLMDITPPDEIFITDTTFRDGQQARPPYTVKQIFDLFILMSRLGGENGVIRQTE
                     FFLYSKKDREAVDKCLSLGLRYPEVTGWIRAAKEDVRLVKEAGLKETGILTSVSDYHI
                     FLKLNKTRRQALDDYLNIVRSILAEGIIPRCHFEDITRADIYGFCIPFAIELMKLREE
                     SKIDIKIRLCDTMGYGVTYPGASLPRSVDKLVRAFIDDAGVPGHLLEWHGHNDFHKAL
                     INATTAWLYGCSAANATLLGLGERTGNPPIEALIIEYIALTGNDHGIDTTVITDIAQY
                     FEKEIGHKIPANYPFVGADFNVTRAGVHVDGLIKCEEIYNIFDTTKLLKRPIVPMITD
                     KSGKAGIAFWINSHFGLHDAAEIDKRHPGISKIHKWINEEYESGRVTSISNEEMEKRV
                     RKYIPELFMSELDKIKYRAAQAAVAVVKQIIEHPAIKTMKPEEQEPVMQQCIEENPSI
                     QFAYVVDMNGRKTTKNITNIQDRAKYENYGVGTDQSDREWFIRPLQTGKIHVTNFYIS
                     KMTGALCITVSAPIMDDRDEMIGIFGVDIKFEDWVKRVEDIAEATQIALRAEYEEKQK
                     TDRWL"
     gene            complement(16999..17469)
                     /locus_tag="PLT63_00095"
     CDS             complement(16999..17469)
                     /locus_tag="PLT63_00095"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOD97149.1"
                     /translation="MQYKRHWMFVCGIVAAAMMVLSFAGCAKKAVTAGPTAAPGDQQV
                     VSETKTVAAAPATTEAKPEAKEAGLVTEYVVKKGESLWWISKYKDIYDDPYLWPIIYQ
                     ANKDQIKKANLIYPGQKLKIPRSGLTMKDVEKARKQAGAKKPYKPSKDSRPPVN"
     gene            complement(17557..19338)
                     /gene="aspS"
                     /locus_tag="PLT63_00100"
     CDS             complement(17557..19338)
                     /gene="aspS"
                     /locus_tag="PLT63_00100"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOD97150.1"
                     /translation="MSAFITKYKRTHYCGALTVSEAGQEVVLMGWTHRRRDHGGVIFV
                     DLRDREGLVQIVFNPEYEADSHQEAHRIRSEYVLAVRGKVCLRPPEMENPELKTGKIE
                     VMIDELEILNESRTPPFLIDAPAAEISENVRLKYRYLDLRRKDIQQKLILRSKAAAST
                     REYFQREGFIEVETPVLTKSTPEGARDYLVPSRVNPGTFFALPQSPQIFKQLLMVSGF
                     DRYYQIVKCFRDEDLRADRQPEFTQIDIEMSFIDENDLMKIMEGFVAHLFRSCLGIEI
                     ALPLPRLSYRQAMERYGRDNPDTRFGMEIMDLTDILRHADFNLFQEVAASGGAIKAIR
                     VEDRRQLSRKDIDQLQDYVADYGAGGLAWARINADRWTSPIAKFLKPVEIRDIETRLE
                     AKSDDLLLVIADTPGVVNDALGNLRLHLARKLNLIDPKAFAFTWITEFPLMEYSDTEK
                     RFTSTHHPFTAPILEDHVYLQSDPGRVRARAYDLVLNGSEIGGGSIRIHKKDVQAAVF
                     AALGLEQEEIRNKFGFLLDALEFGTPPHGGIAFGFDRLIMLMTGADSIRDVIAFPKTQ
                     KASCLLTDAPSQVSIEQLMELSLKIVQ"
     gene            complement(19369..20628)
                     /gene="hisS"
                     /locus_tag="PLT63_00105"
     CDS             complement(19369..20628)
                     /gene="hisS"
                     /locus_tag="PLT63_00105"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOD97151.1"
                     /translation="MELITAVKGFKDILPEETGRWQYVEDQARRIFTAFGCREIRVPV
                     LEKTDLFTRGIGEATDIVEKEMYTFRDRGDECLTLRPEATASIIRAYLEHHLHATDPI
                     AKLYTIGPMFRRERPQKGRYRQFHQIDVEILGHEDPRIDAEVILMLLHFLQSVGMESP
                     HLEVNSLGCPACRPAYRTSVQSFLAGKEADLCDDCRRRLGTNPLRVFDCKIEACREII
                     TKAPSITDFLCEECKDHFAQVLSSLAIFDIAYTVNPRMVRGLDYYTRTAFEITTEFLG
                     AQNAVAGGGRYDGLIRDLGGPDIPGIGFAIGFERLLALTADTSAPPPSRLPDLFIAAL
                     GVSAEQFAYRLCNNLRLRGRKVEMDYQSRGLKSQMKRADKLKCRRVLIIGDREIEAQQ
                     AEMRDMVTGEQVRVSLGDSFAWIDMLV"
     gene            complement(20797..21867)
                     /locus_tag="PLT63_00110"
     CDS             complement(20797..21867)
                     /locus_tag="PLT63_00110"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOD97152.1"
                     /translation="MTEEERPGRETDVPEGNDNLIRELETLYHKVAALDDPDLPPDNV
                     IDFHAAYNILQIAPGASREEIQTAYEKLNEAWREGRYPQVPFWDEKAAYKRREVEQAY
                     ENLIKFHALRHKEMPPIAEAAPAPNLIDKPEVSDQKEIMATPHAEDVVSTDKHSGRWG
                     IWFFFIGVPTLLIAAVAFFWPELYHYDTLTVSQRIFPLRIHRLTGDVAYHDGTGWRRP
                     PLPEVTANPPIPAPATEESPSPPSQLMEMKITAPVSSTTSPIKDKKEIPAEKSSPGQK
                     TYRIQIAAFPDTGSAAAEIGRLKNKYRHVSIEEVSIKGKGVWNRVLVGEFASLEEGRR
                     FLRTEGLDRRYPGSFLRKANGS"
     gene            complement(21864..22613)
                     /locus_tag="PLT63_00115"
     CDS             complement(21864..22613)
                     /locus_tag="PLT63_00115"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOD97153.1"
                     /translation="MNSFRGHTLYEILEVNPDASPYEIRRAYQEIHELYAHESLASYS
                     FFREEERQELLTELEKAYLVLIDERSRRNYDQRLIAQGLLNEKMCYQDRVREPAPLYP
                     FTRDTSARGRKLIRLPHPDDVGEDTTYMNSIETDSVPADETDLVAPETRSTPNPVSIC
                     DLQQKEAISGLDLQNWRLQAGVALEEIAIQSKIKMSMLEALEGDQYHLLPPLAYLKGF
                     LRIYAQIIHCDPEGIIKGYIKKLQTEQGGSP"
     gene            complement(22630..23568)
                     /locus_tag="PLT63_00120"
     CDS             complement(22630..23568)
                     /locus_tag="PLT63_00120"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOD97154.1"
                     /translation="MAIILPIGGGKGGSGKSFLSGSLAVLLAREGFRTLVIDVDLGAA
                     NLHTIIGVTHPRLSLSDFINKRVSNLADTIIATPTPLLFMISGAMNNLDIANLAHEQK
                     MKVLRHIVKLPYDYILLDLGAGTSFNTLDFFMISDHGIFVTTPEPTAIENIYRLIRSV
                     YFRKIRKALDTHHFQKLAREAEERNPMAKVTNPDLLLAVIRELDPERGELLAGELESF
                     QFKLIVNQHRKQDNPHIGTMICKIISRHLNLKIVFLGNVAFDDRVHNAVCKTSLFIDL
                     YPFTQTVHDLRQCLRNLLSSSVVGGKCDLSGSMSDF"
     gene            complement(23716..26295)
                     /locus_tag="PLT63_00125"
     CDS             complement(23716..26295)
                     /locus_tag="PLT63_00125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HOD97155.1"
                     /translation="MGRPLAAPTLALMAGIACSSVYEVATAPLLTALLAACLFLLVCI
                     IRRRSKAASFFLAGAFFVLGWLQMNLYLYREPAGDHIVNLLKSEPVTITGLVSEAPQH
                     FPEKTDLTVKALKMTEGTGAEHPCHGKILISYQGRRTFAYGDAVSARTRLRRVRNFHN
                     PGGFDYERHLKFQGILVRGFIPDDSRIILLRKGLGNPLRQRLEGVRAQIRSFVDLHAP
                     FPEREIVKACILGDQQEIPREIRDAFSKTGTSHIIAISGFNMGLVAFFSIFLVRSLLR
                     RFPYLLLRFEMHKLSVLAAIPPVILYTFIAGAGMSVWRATLMILAFMIALLIARNRDL
                     YNTLAMAALFILVPYPPALFDISFQLSFAAVAAILFITPRLTAFVPKMVSNRSTSSLL
                     AHLIGRLRYQTALFMIVSLSAMLGTLPLIALYFNRISLVAFPANIILVPILGILAVPA
                     SLAAVFFLPWSSTLAKICLDLAGVLVMNSLTVNDFFASLPWSSRYLTTPSILEIAVFY
                     LLIYAVVRLLPQSASPDPAKAATAAKPPLWVHYTLAATVLFFVVNGILLHETGRNHGV
                     FSAAAIDVGQGSSTLVRFPADKIMLIDGGGFPNSIFDVGKYIVAPYLWRERIDAIDIV
                     VLTHPHPDHLQGLHFILNNFSVREVWSTGEKRNSKEYGELLKIIQDRGIPHHRRSSQD
                     DPIIINDVRIEFLNPECSGTDEETESSYVETNDRSLAMRMTFGGVSFLFTGDISGATE
                     EAIVSRRGRILSSLLFVPHHGGRTSTTAGFLEAVKPAAAIISAGYDNPFLLPHPEVLT
                     RLRKAGAKIYRTDLQGMIRAATDGQALFIFPSLTSEGGSPSPQKIEIFVGSPNKYLTS
                     KVN"
     gene            26386..28110
                     /locus_tag="PLT63_00130"
     CDS             26386..28110
                     /locus_tag="PLT63_00130"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOD97156.1"
                     /translation="MTTNDERRWLRYYGAVPPSLDYPRATMYEALMRSAERNPEAIAY
                     DFMGRTSTYRDFTAEIDRCARALYALGLKKGDRITISMPTTPQGIICFYAANKIGAVA
                     SMIHPLSTAQEIAFYLRISRSRFALTIDAFYGKFREVRDDTPLEVLILTKISDYLDLF
                     KKIGFYLAKGRKIPPVPADPQVRWWREIMAASYPPVPEVAMDTEDLAVILYSGGTTGT
                     PKGIMLSNMNFISEGMQVSTWGNLSKQDTILAILPIFHGFGLGVCVNAAFMGGGKSIL
                     VPQFTPEIVAKLIRTKRPTFVVGVPTLFEALCRNPVFQNADLSCLKATFSGADTLPRP
                     VKENFEALVKRQGGKVKLLEGYGLTEAVTAIMATPLQEYREGSIGVPFPDMLAKIVAI
                     DTTEEAPVGAEGELCLHGPAVMMGYLDQPEETARTLRKHPDGRVWLHTGDIASMDQDG
                     FFYFKLRLKRMIKSSGMNVYPAQVEDILYRHPEVKDACVIGVPDEGQVQRVKAFVVLK
                     DMNKAGEAMEEELIRHCREQLIKWSCPREIEFREILPCTLVGKIAYNALEQEEIAKLK
                     AAGKYAGK"
     gene            28320..29510
                     /locus_tag="PLT63_00135"
     CDS             28320..29510
                     /locus_tag="PLT63_00135"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HOD97157.1"
                     /translation="MEEAIKYMAEITAAPLMYGRRIQKETGRRVVGYVCSYTPEEIII
                     AAGAHPLRLFGPMGELECVDTHLQSYCCSVVRGILETGLKGDADFLAGMVFPHTCDSI
                     QRLSDLWRINVTHGFHLDLVLPVKLNTDSARKYYLDVLRRFRMELGKKLGVDIGDEAL
                     RKAYETLREIRRLLAGIYELRSANPTLLSGDDLLTIVKAAMIMDRREASDLLQRVATG
                     LKAKIEAHKSLPESVNSSPPQEKEIKRLVLAGGICNHPRLHGMIEAAGGAVVWDDMCT
                     GARYFQTAGPGVESDGSTAAGKGDTMAAIAERYLNREVCPAKHNGLTSRAEAIISLVR
                     EKQARGVILLHLKFCDPHAFDYPYLKNALDRENIPHLMLEIEDPLPAEGQLRTRFEAF
                     LEMF"
     gene            29524..30759
                     /locus_tag="PLT63_00140"
     CDS             29524..30759
                     /locus_tag="PLT63_00140"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HOD97158.1"
                     /translation="MATNIDKEKRKIKSVKKMKEIMTLYYIDAKTAADNGKKVAWITS
                     GGPVEPLIAMDIIPVYPENHGAMIGAAKMGVDLCEKAEAMGYAGDLCSYARSDISCAT
                     VNGGPIGGLPRPDLLVCGNNICGTVLKWYEIQARYFDVPLFIFDTPFCHTAFHPEMRR
                     YVRTQIDEYIVFLESATGKKLDRDRMVEVGRLSVEGQRLWQAVLDTTINKPAPMSAFD
                     AFFHLALIVTLRGTRQAVDYYQELLAEMNQRVRDGIGAVPRESYRLLWDNLPIWYRTK
                     WLSDKFAAHDACLVADTYTSAWCGSLRYMDENDFLTSMAEGYTRIYLNIGVDEMARMV
                     GEMVDKYDVDGLVMHSNRSCKPYSFGQYDIRKIVEDGKGIPSLIIEADMCDERNFSES
                     QIEMRIEAFIEMLKQKDGQ"
     gene            30770..32620
                     /gene="typA"
                     /locus_tag="PLT63_00145"
     CDS             30770..32620
                     /gene="typA"
                     /locus_tag="PLT63_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HOD97159.1"
                     /translation="MLEKGDGRLLKKEKLRNIAIISHVDHGKTTLVDAMLRRTGAFRE
                     HQAIEDRVMDSLDLERERGITIMAKNTAIRYGKVKINIVDTPGHADFGGEVERSLNMV
                     DGAILLVDASEGPLPQTRFVLKKALERRMPIILVINKIDRSDARIEEVINEVYDLFID
                     LDATEEQIEFPILFTQAKRGVAHRKMGDEAADLGALFETILTAIPGPDGDDEAPPQFL
                     ITNLDYDPYVGQIAIGRLMNGTLEMNRSYSLSGENVVVSGVGFSALYTFSGLKKIAVE
                     KVESGDIVAFAGVEKISIGDTVSALDNPRPLPRIKVDEPTVSMIFYVNNGPLAGREGK
                     YLTSRHLRERLERESLRNVALRVIPTERADAFEVCGRGELQMAILIETMRREGYEFMV
                     SKPRVITKEEGGRVLEPVERVFLDIPEEFVGVVTEKLSIRKGRMANLVNKGRGRVNMI
                     FLIPARGLIGFRSHFLTDTKGAGVMNSLFEGYEPWCGAIPQRLTGALVADRAGRATAY
                     ACHAMADRGELILAPGAEVYGGMIVGERNRSQDLNVNIVKEKKLTNMRSATADATVIL
                     RPPRLLSLDQCIEFIAADELIEATPESIRLRKQELDAQVRMSRYREEKWS"
     gene            complement(32639..34969)
                     /locus_tag="PLT63_00150"
     CDS             complement(32639..34969)
                     /locus_tag="PLT63_00150"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HOD97160.1"
                     /translation="MELAKFFQSIDILADLKKEDLEFLATNSQLIEFPPAALIINTGE
                     IGRFLWLVYEGEVEVTLPDDKGQEKVLASLERGSFFGEMSILTGEPAAANVVSSRSSK
                     VVKIPREVITQVVSRNPKTLMKVTRIITKRLLEDEKFIEEMRRRRLAHSRNEDPYDLN
                     FSSVAEPMKILVVNSGSSSLKYSFFDTTQKESLLDGLVEKIGSGAAVHIIKKAGQKTT
                     LPADGIATVSDAFHAMVRALSDEKQGAVGDVHGISAVGHRVVHGGQRFSSSMTINDDV
                     LTAIKECIPIAPLHNPYNLEGIETLKTLLPGVPQVAVFDTSFHLNMPEAAYRYALPME
                     LCDEEQIRRFGFHGTNHRFVSLSAATSLKIPIGDLKIISCHLGSGASVCAVDHGRSID
                     TSMGMTPLEGMVMGTRGGDVDPGALLHLMRHAGMTYDDLDRILNKESGLKGLSGKSND
                     MREVLAAAEGGDMRCKMALSVFCYRIKKYIGAYVAALGGLDVLIFTGGIGENSPEIRA
                     RICQGMEVFGIAVTDDINRKTVAMRGQIVDISDPSAKIRVLVVPADEERMIARETVHA
                     LGRSLAVSELERLQSKAIPLSISAHHVHLSLDDFTALFGPGRSLTPRSELSQPGQFAA
                     VETVNLVGPKGRIEKVRILGPLRKESQVEIARTEQFRLGIDAPIRDSGDIEGTPGVIM
                     EGEVGTVTLDKGVICAKRHIHISPEEALSLGLRDKDVVMVKVKGVRELIFGDVLIRVN
                     PSYRLDMHLDTDEANAAQISPGAAGYIEAIQHRNYV"
     gene            35270..36061
                     /locus_tag="PLT63_00155"
     CDS             35270..36061
                     /locus_tag="PLT63_00155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HOD97161.1"
                     /translation="MSKQNSRKHIFTLGIDVGSITTKAAMMRDGRLFSYATTFTGYNM
                     EAAVKKVVDATLAEASLVPENLTRIIATGYGRKSVAVAHEDRTEILCHAAGAYFQDSA
                     IRFVIDIGGQDSKAILLDDRGKVSNFTMNDKCAAGTGRFLEVMARAMEVNLDAFGDLS
                     RQADAPAKISSICTVFAESEVISLIAKGTARENIVAGIHDAVGVRVAAMARRIGLRGP
                     VMMTGGVAKNRGVVAALKEKLGLTVTVSPYAQINGALGAAFLAWK"
     gene            36409..38067
                     /locus_tag="PLT63_00160"
     CDS             36409..38067
                     /locus_tag="PLT63_00160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD97162.1"
                     /translation="MLLISRIAMATFLLAIFVFMQNQSPDFASRISIPYFYGIILAIY
                     ILSLFYSVLSRISANHRMQIYFQVMTDVIVITALIYVTGGIRSTYAVFYPLVIIYAVL
                     YLGRNGGLIAASAASICYGLLIDLEFYRLIKPLSYPSMWEIPDTAGHVFYRIFVNIVS
                     FYLIAGLASFVVEKERKTRALLAEKESAFNKLDLLYRSIIESVDAGILTIDMEKRIKS
                     FNRAAEKITGWSFTEVENRGIDEVFTNWESLLENKGSYDRQIQGRIEMTVTAKDGRKL
                     IVGCLPSVLRDHDDHKFGDILVFQDLTAVKQMEEVLERNRRLAFVGEMAAGLAHEIRN
                     PLASISGAVQMLQRETGFSATEARLMQLIIRSKDQLEGFIKDFLLLARPDRGPQQVVH
                     LTDLIKEVMEAVRVTPDWNSAIILREDFEPGLTVSGNREELRQLVWNFVLNAAQAMPA
                     GGTLKLEARQRSNDDRPGVEFITADTGTGISECDQRSIFEPFFTTKERGTGLGLAIVM
                     RIINSYGGKLSFKSVAGRGSTFQVWLPWQENISGGLENGQDSRR"
     gene            38045..39424
                     /locus_tag="PLT63_00165"
     CDS             38045..39424
                     /locus_tag="PLT63_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOD97163.1"
                     /translation="MARILVVDDDLGMRDLLEIMLTQEDYEVACCGDPDKALQRVQKE
                     QFDLVITDLKMPKMDGIEFLKQVKEVKPETQVILITAFASGNTALSAMKEGAYDYIEK
                     NFDIDELKRIVHEAMEKKGIKRQEAQFIKDLADDVAFGGMIGKSREMLKVYGTIKKVS
                     ETPANILILGESGTGKELVAKAIHEHSPRKDMPFVVINCGGIPENLLESEFFGYMKGS
                     FTGAFTDKAGLFESANNGTIFLDEIGELPSLLQVKLLRVVQEKTFRRIGGTLDIKVNV
                     RIISATNQNLEDKVKRGSFREDLYYRLNVIPIHLPPLRERREDIPVLTRYFIEKYSQE
                     FAKDIKTISSYALELLMDYPFPGNVRELENIIERSVALESTNIILPDNLFIDREQVKN
                     DHPHPAGIPEVGIQLNEELARIERQFIEMALNKTKGSKTKAAELLHVTFDSLRYRIEK
                     LRIENSGWD"
     gene            39707..40915
                     /locus_tag="PLT63_00170"
     CDS             39707..40915
                     /locus_tag="PLT63_00170"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOD97164.1"
                     /translation="MTARITKGIIIGVIVGLMVLGVSGWAAAKVVKVGAAINMTGPAS
                     SWGQFHARGQQDYFRYVNEVKGGVAGNRIEMLLVDTAYKVPEAVAAVRKFAVQEKVDM
                     IATWGAGEGLAAKPIVQTYKIPTINYSTSWEILEPPIDYMYLPFGSYKMDCYAILDYI
                     RAVHRGNTIPKVGLLTYNNAYGRSIHKPSREYAKKVKIDIVAVEEFPPKTVDLNTELL
                     RLQKKGAQYVIMQMLPAAIVTTFKSADRIGYNPMFFGTWTSTDPDFFRLGRGLIKDRL
                     LMQFPGGLPVDRTPGIKVMEELWKRYKTVNKFDASYWEGVVVAMIMERAFLRAHEKFG
                     HINRETINKAMESFQNEDFGGLVPNVTYTKKNHGASFKARLVRVQENGSYTPLTKFFV
                     PGKEKIRLLK"
     gene            40929..41741
                     /locus_tag="PLT63_00175"
     CDS             40929..41741
                     /locus_tag="PLT63_00175"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD97165.1"
                     /translation="MKAYRGQPAIFAARKEEERRAHLEIRNVCLNFGGVKALNNIDLQ
                     VGNGELFAVIGPNGAGKTSLMNCITGFYRAVSGQILFNGREITHLHAHELVRVGIGRT
                     FQNIELFPGMTVLANMLLARHLYCGYGFPGASLYLPSVRREEVRHRKLIEELIDFLEM
                     QAIRKKTVASLPYGLMKRVELGRALALEPRLLVLDEPFAGMTLEEKEDMVRFITEINS
                     AWGQTIILVEHDMAIVMSVSHRIAVLNFGEKIAEGTAEEIRRHPEVIRAYLG"
     gene            41753..43678
                     /locus_tag="PLT63_00180"
     CDS             41753..43678
                     /locus_tag="PLT63_00180"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOD97166.1"
                     /translation="MTTKFQDEQMLTLPRLLCKQAEVLGRGAVAIREKAYGVWHTYNW
                     DDYLRYVRHTALGLASLGVRRGENVVLVVNNHPEWLFSELGAQAIGAVTINLFTSSIP
                     EELRTSLQRAQAVIVVAQDQEQVDKLIEIRARIPQVRKVIYLDPTGMRSYREESWLQS
                     YADLLSAGEEKEQREPDYFSREINRGNAKDIALMIQTSGTTGVSKLAMLSHRSMIAMG
                     RQWTQAIALERGENWLSMSPPAWIVDQMWGMGVSLYSGMTMNFPETPETVTEDFREIG
                     PAVIITASRFWEDLASKIRVKMADAGWLKRRLFHWSEMVGAAVIAAPGNGKEPAVLTS
                     LLHRLAGLVIYRPLLDRIGCSGLRNAYTGGHPISPEVIRFFQAVGMNLKQCYGLTEAG
                     GIFQVQPDGEVKPETVGKPLPGVQIMMNEDQEVLVKSEALFSGYYRDFQNTEKAFHDG
                     WLKTGDAGYLDQEGHLVIIGRKEDIIKNKSGQAFSPDFIETRLKFSPYIKEAVVFGEA
                     RPYITAMVNIDMENVGNWAEERLIPFTTYLDLSQQPAVEELIRGEIEIINGQLPEIMK
                     IRKFLLLYKLLDADDEELTRTGKVRRRFIYGIYLPLIEAMYRDVEQVETKGKIRYRDG
                     RVGEITTNVRILTLTMK"
     gene            43696..44574
                     /locus_tag="PLT63_00185"
     CDS             43696..44574
                     /locus_tag="PLT63_00185"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOD97167.1"
                     /translation="MDFFVQLLVNGLSVGFLYGISAMGFVMIFKSSSVLNFAHGELLA
                     IGAYIFLVLATEAQLPIYGAFILTMLACFSLGFIVEKLFLRPLIGEPLIFVIMLTVGL
                     SAMFKGLILLIWGGNLYTFPEFLPEWLALTWGDVTVQPVYTLALVAGVSFLTLFGLFF
                     KFSSQGIYMRSVADNQRAALSLGVNVKNVFALSWAIAALVAGLSGIILGIINGVNVHD
                     LSAIGLKVFPVVILGGLDSIGGAIVGGLIIGLLETLTGGYISPSLRDVVPYVVLVVIL
                     MLKPYGLFGQVEIERV"
     gene            44576..45646
                     /locus_tag="PLT63_00190"
     CDS             44576..45646
                     /locus_tag="PLT63_00190"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOD97168.1"
                     /translation="MGKRIFHPCGNYHERYETQLNVYETDYGRFWTAVGVILLFTVVP
                     LASSPYLLYVVNSIGIAAIAAIGLNILVGYTGQISLGHGAFFGVGAYGAAYLATRWEV
                     PFLVSVPAAGLITAITGLVFGLPSSRLKGLYLTIATLAGQFIIEYVLIHWETVTRGTM
                     GITLPTATIFGLDIAGDRTFFYVIFALLIAMLWLAVNLMRTKYGRAFVAIRDNDRAAE
                     GMGIAIFPYKLLAFAISSFYAGVAGAVWAYYTISITTEPFNLGLSVEYIAMVIIGGMG
                     SMSGALFGAVFITGLNEILRWGTDLMMNMGFVSQMGLSMAPLREFVFGLAIVLFILFE
                     PRGLAEVWRIVRSSFRLWPFSY"
     gene            45659..46450
                     /locus_tag="PLT63_00195"
     CDS             45659..46450
                     /locus_tag="PLT63_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD97169.1"
                     /translation="MLLKVNNISVVYSDVIQVLKGVSLNVEKGKIVSLLGSNGSGKTS
                     TLKAISGLLKPENGKVTDGEIVFQGKGIHNQSPEAITRMGIIQVLEGRQPFKYLTIEE
                     NLRVGTAVRRDKSYRQDLEIVYHYFPALVARRRSKAGYCSGGELQMLVIGRALMAKPE
                     LLLLDEPSLGLAPLIVKEIFDIIKKINEEQGATIVLVEQNANMALQIAHYGYVMENGK
                     IVMEGKAEDLVENPDIKEFYLGVAATGDARAYRDVKSYRRRKRWF"
     gene            complement(46480..48234)
                     /gene="recJ"
                     /locus_tag="PLT63_00200"
     CDS             complement(46480..48234)
                     /gene="recJ"
                     /locus_tag="PLT63_00200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOD97170.1"
                     /translation="MYLKLRNKQSLPVTRWRFSLEKEDLTEHLARELGILPIISKILM
                     SRNILCIDDAKRYLFPSLNDLHNPFLMQDMKKAVHRVIQAITKKEKVVVFGDYDADGI
                     TSTAVLLRFLRPLLPGIDYYIPDRITEGYGLNHNAIDILKRRGIGLIITVDCGVSDQE
                     EVQYAASQGIDTIILDHHEVPEELPPAIAVINPHRRDCSFPFKDLAGVGIAFNFLICL
                     RGQLRESGFWDKRSYPNLKEYLDLVALGTIGDISPLIDENRVFTKIGLDIITEERRVG
                     LKALKEICGIETQVVDSNRASFCLIPRINAAGRVGSPSEAVKLLLTDDLNEARELARL
                     LDGYNRKRQAMEKNILDEILAEIAQTMDPLSRSALVFASSNWHPGVIGIVAARLVDRF
                     YRPAILISIRNGIGKGSGRSILDFNIYQGLKKCDSLLLSYGGHQYAAGISIREEHIGD
                     FRQLLEEVIRENIQPGNMISQTSIDAQCHLRDVNHDLLNQMVMLAPFGSRNPEPILCV
                     RNVNVAASNVVGNNHLRLRLHGDGVSCNGIWFSKGHFVHHLTTGTTSDIAFTPQFNHW
                     NGISEIQLKMRDMSVAEN"
     gene            48362..48547
                     /locus_tag="PLT63_00205"
     CDS             48362..48547
                     /locus_tag="PLT63_00205"
                     /inference="COORDINATES: protein motif:HMM:NF027581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4911 domain-containing protein"
                     /protein_id="HOD97171.1"
                     /translation="MSRKNLALFQFLLEGYGRVAAVTTIDPHIARVRITIMPDFKEEV
                     FEIIARIAKIVACRRIS"
     gene            48601..49020
                     /locus_tag="PLT63_00210"
     CDS             48601..49020
                     /locus_tag="PLT63_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97172.1"
                     /translation="MKRLYRLINVIVILPLLMGMSNLEGESPARIPVPEKKFKAAFID
                     QTDTYTDVSEASIDGHAFLAGKKGEGNYTVAFENIHYIDYLMKNDILIAQIQMRDGAV
                     LSLTVKKNSHAFGKTSYGTFQIRLADLKRMTILGPQK"
     gene            49042..49839
                     /locus_tag="PLT63_00215"
     CDS             49042..49839
                     /locus_tag="PLT63_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97173.1"
                     /translation="MLMMTHTWLLDSYLKEKRLSSDYLDLFIYNICPDLLPLHKGITA
                     SETHGISRFTPLPQNRRKNAFVQFHLMVDDLSHHGMIPTEPVREFNPDSQGYTYLKGK
                     PLVEPLRDIYEFQGTPIPYAQAAYRSHMVIEMTFDLAMHGAESREGERLITLICDAMH
                     FTVEEKLTEFSTDIAWLYGMSPATVAETMRKGAAFYNRARIRSFMSMEGRIQLFAGKF
                     GISREDDQALAMLAEVMNQGMELVRDYDDFMKPALAEIRSAGFNPEY"
     gene            complement(49840..50037)
                     /gene="rpmB"
                     /locus_tag="PLT63_00220"
     CDS             complement(49840..50037)
                     /gene="rpmB"
                     /locus_tag="PLT63_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOD97174.1"
                     /translation="MARVCEICGKRPVVGNNVSHANNKTKRVWRPNLQKIRALDDKNG
                     AVRRIKICTRCLRSGFVRKAV"
     gene            complement(50105..51421)
                     /locus_tag="PLT63_00225"
     CDS             complement(50105..51421)
                     /locus_tag="PLT63_00225"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOD97175.1"
                     /translation="MMTTSDLNFEIPALGSATIPSPITVSYFTPDDRRIIYDNDLRHL
                     SSHPWDLQPPLSIEVAGPRRMIYFDPPKTKAAIVTCGGLCPGINDVIRAIVMELYHRY
                     AVQNIVGIKYGFQGLIPAFGHEVIELSPELVKDVHAVGGSILSSSRGKQDIGEIVNVL
                     KRMNIDIFFCIGGDGTMRATELITEEIARRKLDISVIGIPKTIDNDLNLVDKTFGFDT
                     AISEAVQAIRSAHVEAKGAPMGIGLVKLMGRQSGHIAVHAALAQNDANFVLIPEVPFD
                     LHGERGFLKALEERLRSRLHCVILVAEGAGQEFFFREDDTIETDASGNLKLFDIGLYL
                     KCAIEGHFKNDGLPVNVKYIDPSYMIRSVPANAGDSIYCGALGQYAVHAGMSGKTGML
                     VGLFRGEYVHLPLRAVMSGKKVDPTGNIWMRVIESTGQPLVMKNEG"
     gene            complement(51524..52186)
                     /gene="purN"
                     /locus_tag="PLT63_00230"
     CDS             complement(51524..52186)
                     /gene="purN"
                     /locus_tag="PLT63_00230"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOD97176.1"
                     /translation="MKNKLKLGVLVSGGGSNLQAIIDNIEAGRLDAQISIVISNNADA
                     FSLQRCRNHNVPAIVVDHRDYTSRNDFDRHLIDVLRSYDVELVVMAGFMRILTKSFLA
                     AFPMRIMNIHPALLPAFPGLHVQKQALDYGVRFSGCTVHFADGGVDTGPIIIQAVVPV
                     FDEDNEERLAARILQEEHKIYSRAIQWYAENRLEVQGRKVLLKGNRGGDIRAQHNPPL
                     SE"
     gene            complement(52183..53226)
                     /gene="purM"
                     /locus_tag="PLT63_00235"
     CDS             complement(52183..53226)
                     /gene="purM"
                     /locus_tag="PLT63_00235"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HOD97177.1"
                     /translation="MTTTYRQAGVDIDQANLFVERIKPLVSGTARKEVMGRIGGFGGL
                     FHLDLNRTPDPVLVSSTDGVGTKLKIAQMMNRHDTIGIDLVAMCVNDVIVQGAEPLFF
                     LDYLATGKIGIETGIDIVRGIVRGCLDAGCALIGGETAEMPGFYPEGEYDLAGFCVGV
                     VNSDRIIDGSGIGVGDTIIGLASSGLHSNGYSLARKIVFDMANLSAADRPAGLDVSIG
                     EELLKPTRIYVKALLKLIKSFHIKGLVHITGGGFLDNIPRIVPGPCKAVVYRQSWPVP
                     PIFSLLRDLGKVAEMEMWRVFNMGVGMMIVVSEGDRDEVMERIAMMGEQPFCIGHIEK
                     RSEQEASVQFTDP"
     gene            53498..54808
                     /locus_tag="PLT63_00240"
     CDS             53498..54808
                     /locus_tag="PLT63_00240"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOD97178.1"
                     /translation="MKKITLGLIGFGTIGSGVVKLLRENGGLIERRLGARLFLKKIAD
                     LDITTPRVVPVPPELMTTDAAQIIDDPDTDIVIELMGGYEPARSFILAALANGKHVVT
                     ANKALLATYGNEVFMVADRHDVDIRFEASVGGTIPIVKTLKESLVGNQIDSFMAIMNG
                     TSNYILSKMTDEEKPFDVVLKEAQALGFAEADPTFDIEGIDTAHKLAIALSLAYGKRV
                     DIDEIYREGISGISMQDLEFAKEFGYRIKLLALAFSRGDLVEARIHPTMIPVDAMLAN
                     VNSNFNAFHIIGSSSGPVFLYGQGAGMMPTASAVISDIIDIARGMLHGVSRLVPYRSL
                     GEDIIDDIRLIPFPEIRTNYYFRFMAADKAGVLSKISGILGEMNISIASVIQKGRKKD
                     GAVPVVMTTHKARERDVREALARIDRLDVVHEPTVLIRIEDERL"
     gene            54808..56022
                     /locus_tag="PLT63_00245"
     CDS             54808..56022
                     /locus_tag="PLT63_00245"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HOD97179.1"
                     /translation="MKYIVLLGDGMADYPLERLGGKTPLETARTPQMDFLAREGILGL
                     VDTIPPGFKAGSDVANLSVLGYDPATCYSGRGPLEAANMGVALGPRDVAFRCNLITLG
                     DGGDSTMDDFTAGHITSGEAAEIIASLQDTLGNDEFQFFPGVSYRHLFVWRNGVAEMQ
                     TTPPHDITGQKSNAYLPAGPGSRTAIDVMSRAKVILTSHSVNETRKREGKRPANAVWL
                     WGQGRVPQMVKLTDRYGFRGGVISAVDLLNGIGVYAGLQPLRVEGVTGYIDTNYRGKA
                     ERALAALQDLDFVFVHVEAPDEMGHEGNLPGKIKAIEDFDNLVVGPVLKGMPGLGAVR
                     ILVLSDHPTPISIRTHAADPSPFAAYSTVEKENRPSGRSYGEATARETGLLVTPGSDL
                     MDLFISPGRPFF"
     gene            56019..56438
                     /locus_tag="PLT63_00250"
     CDS             56019..56438
                     /locus_tag="PLT63_00250"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOD97180.1"
                     /translation="MINRTKIRVIYADTDAMGIVYHTNYIKWFEVGRTELFRDLGIIY
                     VEWATEGISLPLTRAYCHYLLPARCDDVVWVETEIAYLKRASVKFRNIILNEDKKRIL
                     AKGYTVHACLNREGKISRIPEKMAAMIGRGIKTETGE"
     gene            56440..57120
                     /locus_tag="PLT63_00255"
     CDS             56440..57120
                     /locus_tag="PLT63_00255"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD97181.1"
                     /translation="MASKMNKQEILTPDKFQVFLETARDYILTHKRNIAVGTAVVTLI
                     IAMVAGWAYWRQTEETKAANLYNQAVLNNSRLAVMNQDVAATVKPFQEVTNKYPGSKA
                     AILSQYRIGNAYLNAGRTDEAIKFFQAYLKGKSGDNELTALAYNGLGYCYETMGDFKL
                     ALNNYERALQSRAGGPFAGDILAGMARSYEGMQDKDKAREYYRQAMEKVQDPTLKMLI
                     GRKLAMLG"
     gene            57146..58942
                     /gene="iorA"
                     /locus_tag="PLT63_00260"
     CDS             57146..58942
                     /gene="iorA"
                     /locus_tag="PLT63_00260"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HOD97182.1"
                     /translation="MKVLMSGNEAIARGAYECGARFGAGYPGTPSTEILEEFSKYEGV
                     YAEWAPNEKVALEVGIGAAVAGAKALVTMKHVGVNVAADPLFTVSYTGTNGALVVVSA
                     DDPSLHSSQNEQDNRNYAKFAKLPMLEPADSAEAGRFVKIAFEISAAFDTPVFLRSTT
                     RVSHSKSVVVTETPVPYEDRTELRINPSKYVMVPAMARIRRVEVEKHLQALREFAETF
                     PENRMEINNTEMGIITAGMPYNYAKDVLPNCSYLKLGLVHPLPEKLIRKFASLVKKLF
                     VIEELDPFLEEQIRAMGIEVTGKELFSYTNEFDPGVIAGAMMKKTDAAVKLPEVAIPP
                     RPPNLCPGCPHRGLFHVLGKMKTTFVAGDIGCYTLSFMKPLEGLHSCVCMGASIGMAH
                     GMSKALGEKGRGKVVGVIGDSTFIHSGITPLLNLAYNQGDAVIVICDNRTTAMTGMQE
                     HPATGYTLQGAKAKELDFQALAAVLGIDSVRVIDPYDLKNTRTVMKEELARSGSSLVV
                     SRRSCVLFKRRYTETRKPLRVNVNKCIGCRNCLDLGCPPIAWRSFKDQPANEIIKNAK
                     KQEGNSYIDADLCTGCTLCRQVCKVGAIEEVE"
     gene            58946..59566
                     /locus_tag="PLT63_00265"
     CDS             58946..59566
                     /locus_tag="PLT63_00265"
                     /inference="COORDINATES: protein motif:HMM:NF005322.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOD97183.1"
                     /translation="MDKKGKKAGKNITGRVISVLMAGVGGQGILRASDILCRVIMEAG
                     LDVKKSEVHGMAQRGGCVTSHVRYGQKVYSPIERKGNVEILLAFEKLEALRYLDYLKH
                     SGRIIINEVEIYPPAVNLGSMTYPDNAVDLVRHKFSTVMVVNGRDLAAASGNIRTENT
                     VLLGALSQWLDFELDIWERVLKESFPPKALDANLQAFHLGRDYREG"
     gene            59760..60713
                     /locus_tag="PLT63_00270"
     CDS             59760..60713
                     /locus_tag="PLT63_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HOD97184.1"
                     /translation="MAEDYYQLLGVDKKASAEEIKKAYRKLAFKWHPDRNPNSKAQAE
                     EKFKKISEAYAVLSDQEKRQQYDMYGSADQFRQKYTQEDIFRNFDLNDLLRSFGFDFT
                     GGGGRTKGFSTGSRRRGGFQQSFDPFADLFGGGGQSYGHMPRQGQDLQYNLSITLEES
                     VQGVEKKLALQREDRVDEINVKIPAGIGAGKKLRLVGKGAPGFDGGPTGDLYLHINII
                     PHPLFVRDGNDLFVEKTISFTQAILGTTIDVPTIDGGTKRLKIPPGTQNNTKIRMKGY
                     GIPGLKGGLKGNQYVKINIQVPKTLTERQGKLVRQLAEEGL"
     gene            complement(61077..61799)
                     /locus_tag="PLT63_00275"
     CDS             complement(61077..61799)
                     /locus_tag="PLT63_00275"
                     /inference="COORDINATES: protein motif:HMM:NF003323.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HOD97185.1"
                     /translation="MEYSERTYRQAVTASGLFTFQVAVRETDLFIAADTDLTEQAWTS
                     ILKHRSHLESYIKKYPVFRHSLTPLPNDALAPPIVRTMLAAANTAGVGPMAAVAGAVA
                     DYVGRDLLAQTKNIIVENGGDIFMHTAAGRRVAVFAGQSPLSGKLTLEISPEDMPLGI
                     CTSSATVGPSLSFGKADAVCVLSSSAALADAAASQIGNLVRRAENIKKALEVGGAIQG
                     VQGIVIILGDKLGVWGKIKFSE"
     gene            complement(61873..65340)
                     /locus_tag="PLT63_00280"
     CDS             complement(61873..65340)
                     /locus_tag="PLT63_00280"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOD97186.1"
                     /translation="MKHAPFVHLHVHTQYSLLDGTIRIEDLLKRAKEYQMPAVAMTDH
                     GNVFGAIDFYQQAYRHGIKPIIGSEMYVAPGRRTDKNTPGSGENSRHLVLLVKNMNGY
                     NNLLKLSSDSYREGYYYRPRIDKELLAAHHEGLIGLSACLHGEIATHIIRQDMDAALR
                     AAAEYRDLFGPDNFYLEIMENGIPEQTTANRGLMEISKKLSLPLVATNDCHYLKRADA
                     EAHEILLCIQTGKTIEDQDRMRFQTDQFYFRSPAEMQELFSYCPEALSNTVVVAEKCN
                     LSLEFGQFYLPRYHLDGERSLDEHLAALAKEGLARLLPAINTTGDEDTVRRYEKRLSE
                     ELIMIKNMGFAGYFLIVADFVNYAKNNGIPVGPGRGSAAGSLVAYAIGITKVDPIRYG
                     LFFERFLNPDRKSMPDIDIDFCQERRDEIIRYVTEKYGRDNVAQIITFGKMLAKAVIR
                     DVGRAMNIPYGEVDAIAKLVPNVLNITLADALKMEPRLQEEAKKNERIQKLLTYSQAL
                     EGLNRHSSTHAAGVVISDVPLDNRVPLYKGPKDEGVTTQFSMNDIQAVGLTKFDFLGL
                     KTLTVIKNTLQLVRENRGIDIDIDSLSLDDKETYELLQRGNTDGIFQLESSGMREILV
                     GMKPDCIEDVIALIALYRPGPMNMVPDFIARKLGKTPISYVIPELKDILKETYGIILY
                     QEQVMQIASAIGRYTMAEADTLRKVMSKKKDQEMEKEMPKFISGAKQNRIPEEKAIRI
                     WEQMKEFAKYGFNKSHSTAYAIVSYQTAYLKAHYPEEFMAALLTSEKDNRDKIIRHIN
                     SCKEMGISVLPPDINESRSDFTVPGSHVRFGLAAVKNVGLGAVEAILEARHKGGKFKS
                     FHDFSRRVDLRKVNKRVIDSLIKCGAFDSLGHKRRTLFGAFEEIMNTAQKLRKAKSSS
                     QTSLLDGLEVVGQGRAGAHNEEIPDLGEWEHKELLMYEKEALGFYITGHPLLRFQEKL
                     SFITNANSENLSERRDKEDVIIAGIVSGIRELTTKRKDVMAYVTLEDLYGSMNIICFA
                     DVYQRGRELLKSDEPLLIKGQTDVSEENVKIIANEITLIQDAPDVSPYTSVHFYIDSQ
                     NSADDNNILNIKYLSNKYKGTSDGYIHLLNGVSETVVYLGNDCRFHLTSELLKAARLI
                     LGPQSSFYR"
     gene            complement(65343..66803)
                     /gene="guaB"
                     /locus_tag="PLT63_00285"
     CDS             complement(65343..66803)
                     /gene="guaB"
                     /locus_tag="PLT63_00285"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOD97187.1"
                     /translation="MRTDNIGEALTFDDLLLVPAESSILPKDVDTSTHLTNNIILRIP
                     ILSAAMDTVTESKTAICMAQEGGIGIIHRNMSIDRQVIEVDKVKKSESGMIVDPITIS
                     PEQKVQEALHLMTEYRISGVPVVKGKRLVGILTNRDLRFETDLEQPVANVMTKDNLVT
                     VTSNISLEESKKLLHKHRIEKLLVVDDEYNLKGLITIKDIEKIRKYPNASKDSLGRLR
                     VGAAVGILDREERIQALIAAGADVIVIDTSHGHSRGVLNAIRDTKLNFPKCELIAGNV
                     ATAEGAEALIDAGVDAVKVGVGPGSICTTRVVAGVGVPQMTAIRETFKVCSRRGIPVV
                     ADGGIKFSGDIAKALAAGAHTVMIGGLFAGTEESPGETILFQGRSYKVYRGMGSLEAM
                     KSGSRDRYYQDDMESNLKLVPEGIEGRVPFRGSLSASIYQLIGGLKAGMGYLGCRTIP
                     EMHEKARFVRITSAGLRESHVHDVIITKEAPNYWLD"
     gene            complement(66846..67313)
                     /locus_tag="PLT63_00290"
     CDS             complement(66846..67313)
                     /locus_tag="PLT63_00290"
                     /inference="COORDINATES: protein motif:HMM:NF018624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1285 domain-containing protein"
                     /protein_id="HOD97188.1"
                     /translation="MAKSPPCQIRIDKEGVWYYQGAEMFRREFVNFFYQHLHRDATGR
                     YVIELPNDRCYVEAEDTAYFIKAVYPQRDGNGAVNNFRIYLCDDTVEDLDPETLKIGA
                     ENVLYCQVRGRSFPARFTRAAYYQLAEHIEYDDGRNAFYLPINGRIYDLDTKS"
     gene            complement(67338..67787)
                     /gene="dtd"
                     /locus_tag="PLT63_00295"
     CDS             complement(67338..67787)
                     /gene="dtd"
                     /locus_tag="PLT63_00295"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOD97189.1"
                     /translation="MRAVVQRVERAAVAVGGREISRTGRGLITFLGVEKGDADKDADY
                     LVDKIINLRIFEDIDGKMNLSLTDIGGELMIISQFTLLGDCRKGRRPSFYRSEEPEKA
                     KDLYDYFVAAAADRVREIGTGEFQAMMKIDLVNDGPVTILLDSRNTI"
     gene            67915..69324
                     /locus_tag="PLT63_00300"
     CDS             67915..69324
                     /locus_tag="PLT63_00300"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOD97190.1"
                     /translation="MPRKTHCLQVSFLLCRLSMKPYVLLVNPWIYDFAAYDYWMKPLG
                     LMYMASYLRKNGLDVRLVDCLDGYAPQAASMALRSRRPPRRKPGGHGKFLRQRIAAPP
                     PLGMIARPYHRYGIDPEVFYAMVRSSGKPRAIMVTSMMTYWYPGVIAAVRILKDIFPE
                     SPVILGGIYATLCPEHARAHTGADYVLTGAGEGHMDLILETILGISASYRPDTDNLDA
                     LPYPAWDLLPHVDQLPVLTSRGCPCRCPYCATRILNRKFRRRRPWLVVDEIAHWRKTL
                     GVKNFSFYDDALLVHPEEMAIPLLKDIIIRNLDAGFHCPNGLHLREITPDIATLLFRA
                     GFRTLRFGLETTDRDRQRALGGKVENVHFEQATAYLREAGYKSDDIAVYLLCGLPGQS
                     AGEIRESIRYVQSHNARPILAEYSPLPGTELWDAACQSSPYPLAEEPLFHNNSLLPCA
                     HPTLTTPEYHELKRRSRGL"
     gene            69414..69674
                     /locus_tag="PLT63_00305"
     CDS             69414..69674
                     /locus_tag="PLT63_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97191.1"
                     /translation="MKKDQIIGTWLWWCKLLAIGGISLLILLWGISALYNAYLLKNPQ
                     EFVMIFFSASFMILLGITGLIYPAYRIHAYFKNDSFEKFPEV"
     gene            69778..71175
                     /locus_tag="PLT63_00310"
     CDS             69778..71175
                     /locus_tag="PLT63_00310"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOD97192.1"
                     /translation="MKSFTLKNGIRVLILERHFSPTASFYIRFRAGAAEEQEGKTGLA
                     HLLEHMMFKGTKTIGGISSVREEKLLREIGKTGETLERERTKGERKDGQRIKALEKKL
                     AALQKAHRRWYRSNEIDRLYAEAGAININASTSQDMITYHVSLPANKAELWARIEADR
                     LANYVLREFYQEREVVKEERRQRIDADPDGLLFERFAATAFMVHPYRRPVIGWPDDIA
                     RLDMEDVDSFHRLAKVPRNMVIAIVGDIRINEMRRLLEKYFGVLPGQPAVFTPVATEP
                     PPRGEKRITVHFDAGARMIMGFLKSPPPNREDYVFDVIEALLSRDRSSRFYRQLLETQ
                     GLAESVQAVNGLPGNRYPNLFCVFASVRHPHKIDELAAAVEKIIDDLAVEPVPAAELE
                     KVKNQLQADFIRRQDSDEGLASLLSYYEALLGDYRYLSSYGNQISTVTADEIMAAARK
                     YLNRDNRTVAIMTKD"
     gene            71184..72704
                     /locus_tag="PLT63_00315"
     CDS             71184..72704
                     /locus_tag="PLT63_00315"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOD97193.1"
                     /translation="MLLLINMSCKRNSINFYGCLAGMAQGRRRQGGNFIRTVLSVLFL
                     AAMILTAETPVLAGLPLPDPNRLSYPPLRFSPPVPQHFTIENGLEVYFLANRELPVVK
                     ATLLVKSGAMYDDRGKEGTAELTATLMRTGGTKQIPPRILDDTLASLAATIEPSAGME
                     QIQWSFFSLRKDFSRAWDIFHQILRAPRFDPERLALLKELKSEEIRRLNDDPQQWALK
                     EFFRHLYQGNPRGRLPTVSTLNGISRYEIEKCHRLHYTPDNMFLAISGDIDREEVERI
                     VRETFGSWPAKSGRIAVPPPLAAFQTGKYYLIKDTPQTVTVIGRPAPTKKSPDFFAFD
                     VVDHILGSGGFRSRIFQQVRTDRGLAYATGSFYRAREDYGIFGAYALTKTSAAGEALT
                     VMQDLVRTVRLSGITAEETARAKSSILNSFIFSFATPHQLVHQQASLAFEGLPENFLW
                     QYRKRIDCLTSTEINHVASKWLDFDQALIFMLGKRAGYEEIKKIHPDFKIIKVSYD"
     gene            72697..73941
                     /locus_tag="PLT63_00320"
     CDS             72697..73941
                     /locus_tag="PLT63_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HOD97194.1"
                     /translation="MIDEKAFLRVASRIDAYRQEMIDLQIALTAVPAIAPENGGDGET
                     TKAELLLEYLSHRHIGEIEVVNAPDTRVSSGGRPNVILRRAGGDATRHTWIISHLDIV
                     PPGEAGLWDADPYQAYVRNGRLYGRGTVDNQQDLVASLFAFRACCEEAVPTYRAPGLI
                     FVADEETASHYGLDFLLQHPRNPFQKTDLICVPDSGNEAGDLIEVAEKSILWVRFRTL
                     GKQCHGSKPYLGKNAFLAASHLAVRLHAALPQRFPAVDPLFDPPESTFEPTRRDANVP
                     NVNTIPGEDNFYMDCRILPGYELQTVKEAMTETARTIERELGVTIEILPVQEVQAPPA
                     TDASAPIVIALQEAIARVYGVQARPQGIGAGTVAAYLRRKNYPAAVWCRTTPMAHQPN
                     EFCLIDDMVGNAKVFAHLFLQR"
     gene            74159..75148
                     /locus_tag="PLT63_00325"
     CDS             74159..75148
                     /locus_tag="PLT63_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOD97195.1"
                     /translation="MKASIQDLHTETLQFPDTVQLRNLLGEQDKHLRQLEKLAPVRIS
                     ARGNDLSLSGDVSSVQLIRDLLIQLYGIIEKGYPLFSQDINYAHRILAQDGKASIEKI
                     FLDKVFVSAKKRVIAPKSMAQKLYIDAIRKYDMVFGIGPAGTGKTYLAMAMAVSALME
                     KKVTRIALARPAIEAGEKLGFLPGDLAEKVNPYLRPLYDALFDMMDMDKATALLHKGI
                     IEVAPLAFMRGRTLNDAFVILDEAQNTTSEQMKMFLTRLGFGSKAVITGDVTQIDLPQ
                     EKTSGLIEAEKILQHSERISFIHFSELDVVRHPLVQEIIRAYNHLDQMRSKAH"
     gene            75161..77584
                     /locus_tag="PLT63_00330"
     CDS             75161..77584
                     /locus_tag="PLT63_00330"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOD97196.1"
                     /translation="MITWKEIAAKIVVEIQKPFAHPDLIPMFFKDPAKQRWLILILLS
                     IVLTVMLLPTVTIMAPHYKIGAIADKNIKADRDFLVEERTATELKRLEAAKSSRNIFD
                     LDDTVLPQIRGNIMKAFLAVQEAVKKTAAGTTEASRTASVQKQKILVVMEQYLGVRLS
                     DQDYSALSSHRFSSEIADRIAEIITKVYQDKMITNVDFSPLEYERGLIVKNVRTRMER
                     EEKNADEILQMQDAKWILRQKALATMAEDDAVLKKTVINIAEKLLQPNLTYNRLESEQ
                     RRQSAVEAVKPVYFEVQKNEMIVREGEKISVADLDKLNAFFREQRQQKTSRYAGFMGM
                     FLLIFSLTTALLFLSRKAFGSQDIQPGPNPRLLCLTIIIIIQVALIKAVLFMSDAVHQ
                     AMPILSKEAIIFSIPFAVGPLLTAYLFNRRIALLMSLFISFLIPFLITLPEAYLSLFS
                     FLGFVVIICHNDHYQRRSVFIKTGLLLGISNGAFILAIALLSAHPLSVDTIWKIVSGF
                     AGGVMSGIIVAGISPIFETAFDFTTDIRLLELANLNQPIFQRMIMQSPGSYHHSIIVG
                     SMVEAAAETIGADPILSKVSAYYHDIGKMKKPVYFIENQDRGENRHDKLSPRMSALII
                     IAHVKDGCDLADDYKLGSKITDIIRQHHGTSLVSYFYEKAKRDKAPSMRSLPESHFRY
                     HGPRPQTREAALVLLGDVVEASSRTLVDPTPARIRNLVRDRMDKVFMDGQLDECSLTL
                     KDLNLIQDSFVRTLNGIFHHRISYYDDVLGKNNKQVIKSFYEYPDRKPAEKITHKSPA
                     DSQQHGETA"
     gene            77568..77957
                     /gene="ybeY"
                     /locus_tag="PLT63_00335"
     CDS             77568..77957
                     /gene="ybeY"
                     /locus_tag="PLT63_00335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOD97197.1"
                     /translation="MARLLRYLGHSDAEVSMLLVDDEGIREINRSYLQRDYSTNVISF
                     AMREGPYGQLHPQVLGDIVISVETACRQGDKGALSMTESLSFFMIHGLLHLLGYDHEG
                     VDARDIQRMEEKEDELFFLLHGFSLQR"
     gene            complement(77967..78866)
                     /locus_tag="PLT63_00340"
     CDS             complement(77967..78866)
                     /locus_tag="PLT63_00340"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOD97198.1"
                     /translation="MRIRIASKVNERLWYLGRMESGMYLLSVNGEAMLISAGLSCILE
                     DVLAQMAAFGIEGEKITSLLILHSHFDHVGTVPYFQRKYPALKVYASQRAWDILTMPK
                     AVETMNAFSRMVAEKMGCLDVLARHDLDWRDDVQGTIVRDGDLLKVGDLEVRIMETPG
                     HSSCSISAYIPELKTLLPSDAGGIPIDDAIFTSANSNYTLYQESLERLKDLEVEYFGA
                     DHHGYIVGPEARDYVLQSIAAARKLRTRIETVYRETGDIDRASRLLTEELYTRHPNYF
                     LAPEIIVGVFGQMVKHIAKAMNG"
     gene            complement(78879..79862)
                     /locus_tag="PLT63_00345"
     CDS             complement(78879..79862)
                     /locus_tag="PLT63_00345"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOD97199.1"
                     /translation="MNESAPAAEFKPAPTPNPQTSPVAVNRLPTDIVAEVHGVKMTRA
                     QLDADVKKRMAALSPSLSPERADRMRGMMRKQIIDDFVLQTLMLKEVTRLKITATEGE
                     IDAALAEMKAGLPSGASLDDLMKKNGINREQLRKEIALGIKINKLVESQKRVKSPPTE
                     KEIQEFYRRNQDKFKIPEAVHVRHILVAKKDGENDEARAIRKGKAEKLRQQILEGKDF
                     GELAKTNSDCPSKAQGGDLGTFIRGQMVKPFEDAAFSRRKNEIGPIVETEYGFHIIQV
                     LEHHQARTQQLDQDTKLRIAGFLKEKKKYDAFNQIVGQLKEKAHIVVGDIS"
     gene            80207..81571
                     /locus_tag="PLT63_00350"
     CDS             80207..81571
                     /locus_tag="PLT63_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOD97200.1"
                     /translation="MKKKVLDLGVGPILPLLMKMSWPSMLAMLSMAIYNVMDSLWLAR
                     LSPRTIAAFTITFPLQMVFAAIGVGIGVGAGSYASRMFGAGEADKARRTAGQVIFLSV
                     IAGLSLIAVTYAFPDFLLRLFGASGETLVLAKRYLMWVVLSSPFLLFMMMANNLFRAE
                     GLPNLSMYAILTFTFLGALLDPLLIFGWGGFPALGIEGAALAAIVGQVASGLLSLYFL
                     LYRSLRYRIDLQDLLPDPTIIRAIHQTGLPSVLMNLVFSAVIIIYNNILAYYGHLALA
                     TLGICFRINGLVMMVLFGIGHGVMPMVGYNLGAKLYDRLIATVQVAVRCSMLFAGASS
                     AIIFIGADLILSSFTDDRELLVIAAPALKIFISTLVLAAPSIVWINLFIGLGKGLTAM
                     TLIVIRDALFLIPLMYLFSLWLGRDGVWMAQPASTAVAFLIIWVWSGKELAAIRNLKE
                     QTNP"
     gene            complement(81794..81994)
                     /locus_tag="PLT63_00355"
     CDS             complement(81794..81994)
                     /locus_tag="PLT63_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HOD97201.1"
                     /translation="MAEGKVKWFNEKKGWGFIENDEGGDVFVHFSAIQGSGFKTLFES
                     QRVSFDIEQGKKGPAAVNVQTL"
     gene            complement(82222..83202)
                     /locus_tag="PLT63_00360"
     CDS             complement(82222..83202)
                     /locus_tag="PLT63_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOD97202.1"
                     /translation="MMDAPLLSVQRIDKSFAPKRKLFSSAPDACHAVREVSFEIHAGE
                     TLGLVGESGCGKTTLARMVACIERPSSGKIYYRGIDIFVAKKAELHEYRQKVQYIFQD
                     PYASLNPRMSAGDIVGEPFRIHKSRIDGVSLGDLDGLLKMVGLSSEQKNRYPHEFSGG
                     QRQRLGIARAIALNPEIVIADEPVSALDVSVQAQILNLLKDLQDRFNLSYLFISHDMG
                     VIRFMSDRVAVMYGGGIVEIGPNEGIFGNPRHPYTRILMNAVPSFSGQTVKREREIVV
                     SLGEVETIETGCLFCSRCPEALPVCWETAPELAVVSGGHLSACHRSKREV"
     gene            complement(83204..84175)
                     /locus_tag="PLT63_00365"
     CDS             complement(83204..84175)
                     /locus_tag="PLT63_00365"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD97203.1"
                     /translation="MLKVEGLSTFFDTRRGVIRAVDDVSLQVEVGDTLGIVGESGCGK
                     TVLSLSIMKLVPTPPGKYVGGKILLGNHDLLRLPEKEMRKIRGKSISMIFQEPMTSLN
                     PVFRIGDQIREALGLHQGGTRREVWERAVEMLRTVGITKPEQRMRDYPHQLSGGMRQR
                     VMIAMALSCRPRLMIADEPTTALDVTIQAQILELMKQLQRELGTAVILITHDLGVISQ
                     TARQVAMMYAGQFVEYAEAGVIFSRPLHPYTVGLLESIPCIGGKFGPGKRLPMISGGA
                     PDLAAMRSSGCRFHERCVDVMPVCRNLEPPWRKNDHPHQYVRCWKYH"
     gene            complement(84200..85720)
                     /locus_tag="PLT63_00370"
     CDS             complement(84200..85720)
                     /locus_tag="PLT63_00370"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002074.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HOD97204.1"
                     /translation="MKSELKVTVRNAVAGTDQDWGCLGIFVFQGERFEAADNWPVGQE
                     TRDLIRKILKRNDLAGKLYRETVFFPEHGPAPPMIILIGLGKRDEFTLDKLRGAAAKA
                     GKAAQSHREEKLILLLDPSYLRIEGPEAAGAAVEGVTLGLYRFSAFKTLDREEGQDLQ
                     FLTILVPERSRLKAVRTEVAVAVKICRAAYLARDLTSMPANELTPAVLAEKAQILAEG
                     RAKLTVTILSVPEMKRLGMNSLLGVASGSDQEPKFIIVSYLGGDEATSPVVFVGKALT
                     FDSGGISLKPAEKMDEMKSDMAGGAAVLGAVNAAADLELPINVVALIPATENMPGGKA
                     CRPGDILRSMSGKTIEVINTDAEGRLILADALHYASRFEPAAIIDVATLTGACIVALG
                     EQVTGMMGADGPLKEEIKRASQQTGEMVWELPLWENYHEQIKSEVADFKNSAGRTGGA
                     ITAAAFLSKFVGPYPWVHLDIAGPAWLTKETAYQPKGASGVGVRLLVRFLRNRCRK"
     gene            complement(85900..85975)
                     /locus_tag="PLT63_00375"
     tRNA            complement(85900..85975)
                     /locus_tag="PLT63_00375"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(85940..85942),aa:Cys,seq:gca)
     gene            complement(85984..86058)
                     /locus_tag="PLT63_00380"
     tRNA            complement(85984..86058)
                     /locus_tag="PLT63_00380"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(86024..86026),aa:Gly,seq:gcc)
     gene            complement(86120..86938)
                     /locus_tag="PLT63_00385"
     CDS             complement(86120..86938)
                     /locus_tag="PLT63_00385"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOD97205.1"
                     /translation="MAEISDYRTFDRPEILRVLFYPRPDWSLTMNHREATSLMVPVDD
                     GVYVQVQAYLESPAAANIIFFHGNGEIASDYQDIAPFYTDRGINFLIADYRGYGVSSG
                     IPTVSAMMTDCLVIFDHVKGWLREKGFTGLMIVMGRSLGSASALEIAHRRQHDMDGLI
                     IESGFAYLGPLLRLMGVEMEYFHLDEVQGPRNISKIKNISKPTLIIHAQFDQIIAFNE
                     GEALYEASPAPIKRMLRVPGANHNNIFFAAMTEYMAAVLWLVDRAEESRDNETS"
     gene            complement(86931..87551)
                     /gene="pgsA"
                     /locus_tag="PLT63_00390"
     CDS             complement(86931..87551)
                     /gene="pgsA"
                     /locus_tag="PLT63_00390"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HOD97206.1"
                     /translation="MIDHLQRYFDSLPLSPERKKIFNLPNTITMVRISFVPVLFLLLL
                     SPGQTMSLIIAALFIAVSFTDMLDGYVARRYRIVTTLGKFLDPIADKLVINTAMILMI
                     PIGRIPAWVVAIIVIRDFAVDGIRNIASSEGYIIQASWLGKQKTLCQVFAVSALMIHY
                     PFLGADAHVVGTTILFIALILSVYSGLDYLTKFYRHILLGKEGTDG"
     gene            complement(87637..88284)
                     /gene="fsa"
                     /locus_tag="PLT63_00395"
     CDS             complement(87637..88284)
                     /gene="fsa"
                     /locus_tag="PLT63_00395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HOD97207.1"
                     /translation="MKFFIDTANIEEIKEAASFGMVDGVTTNPSLIAREKKTFAEVVR
                     AILKEVSGPVSLEVVSLEAGEMFKEGKKLAKLGDQVVIKVPMTLDGLKATRLFAAEGI
                     PVNQTLVFSPLQALMAAKAGAAYASPFVGRLDDISHDGMALVEQIINIYDQYGYETEV
                     IVASVRHPGHVLEAAMMGADIATIPFKVIKQLAQHPLTDRGVEAFLSDWQRVPKK"
     gene            complement(88281..88571)
                     /locus_tag="PLT63_00400"
     CDS             complement(88281..88571)
                     /locus_tag="PLT63_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97208.1"
                     /translation="MILRLIIAIIVFYLVYRLTRMLFLPGKKTVNPISKEQRENLTAG
                     EELVEDPYCHTYIPISNACIWRQNGKILYFCSRNCMENYQNASQQEKNKEGS"
     gene            complement(88599..89105)
                     /gene="folK"
                     /locus_tag="PLT63_00405"
     CDS             complement(88599..89105)
                     /gene="folK"
                     /locus_tag="PLT63_00405"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOD97209.1"
                     /translation="MTAAGTIAYIGLGSNLQEPLFQCRLALEKLRGMTGVKLLRASSF
                     YRTEPVGVRNQDSFVNAVVEIRTILSPRRLWENLRATEQVMGRQETQRWGPRLIDLDI
                     IFYGQELLREDDLVIPHPECHRRRFVLIPMCELAGYLVHPAFGISVQGLLIRLNEDDR
                     AAVELISQ"
     gene            complement(89107..89916)
                     /gene="dapB"
                     /locus_tag="PLT63_00410"
     CDS             complement(89107..89916)
                     /gene="dapB"
                     /locus_tag="PLT63_00410"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOD97210.1"
                     /translation="MIKTILMGAGGRMGGRIGHLITAATDMEVSAAVEATGHPAVGKD
                     LGEVLGMAMTGILVADDLKKVVKEGDVVIDFTYHESSLRHLEIAAKVGKPIVIGTTGF
                     TPNELKTAAKLAKKTPCVIAPNMSVGVNVMFKVLENVAAILGDDYDVEILEAHHKLKK
                     DAPSGTAMKMAQIIAAKLGRNLDRDGVYARKGMIGERTKAEIGIQTLRGGDIVGEHNV
                     MFVTNGERLEFIHRAHNRDNFARGALRAAKWIVNQQKGLYDMQDVLGLKTR"
     gene            complement(89929..90804)
                     /gene="dapA"
                     /locus_tag="PLT63_00415"
     CDS             complement(89929..90804)
                     /gene="dapA"
                     /locus_tag="PLT63_00415"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041584832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOD97211.1"
                     /translation="MFKGAIVAIVTPFKNGRVDEEKLRELIEFQIENGTDGIVPCGTT
                     GESPTLSHEEHDRVIEITVDAVKKRVPVIAGTGSNSTAEALRLTEHAREVGADGALMV
                     CPYYNRPTQEGLYQHYKTIAEKVKIPVIVYNIPGRTGVNLLPETMARLAKVPGIVGTK
                     EASGSLKQMHEVIQLCGPDFAVLSGDDFFTYPLLCLGGHGIISVISNIVPKDMAALVD
                     AFAAGDLQKAKNLHYRMAPLVDSLFIETNPTPVKAALAMMGKIEYEVRLPLAKMSEAN
                     YEKLRKIMINYGLLR"
     gene            complement(90797..92089)
                     /gene="lysA"
                     /locus_tag="PLT63_00420"
     CDS             complement(90797..92089)
                     /gene="lysA"
                     /locus_tag="PLT63_00420"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOD97212.1"
                     /translation="MHDFHYQDREMWCEETPVAKIAAAVGTPVYIYSHRTLKNHFRVF
                     DDAFDTIPHITCFAAKANSNIAILKIFIREGGGVDIVSGGELYRALRAGVDPRKVVYS
                     GVGKRIEEIEYALETGILMFNVESFQELEIINACAGRLKKKAGVALRVNPDVDPETHP
                     YISTGLKDNKFGINVEKSHQAYLQVRKYRHLDLKGVSCHIGSQLTKMSPFIDALDRLK
                     KLLQVLRAAGIDIRYLDLGGGLGITYKDETPPHPSEYADAIIGASRDISCTFIFEPGR
                     VIVGNAGILVTAVLYTKENEEKKFIIVDAGMNDLVRPSLYKSYHNIQQVRKSRRREII
                     ADVVGPICESGDYLAKERKLPGFARGDLMAVMSAGAYGFTMSSNYNSRPRAAEVLVMG
                     DRYHVIRKREDLRELIRGEKIPDVLKSNAKSILRRAYV"
     gene            complement(92116..92529)
                     /locus_tag="PLT63_00425"
     CDS             complement(92116..92529)
                     /locus_tag="PLT63_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97213.1"
                     /translation="MIGSIAGCGKKGDPVPRQQQPPARISDLTAAAAVDGVLLKWTIK
                     SVPELDSEGKYFQIWRASWDLEKDECPGCPKDYILLIKAPAANFAGEDGGTESYRYLD
                     RSVAKETHYGYRLEWGSPGGSVIAISNPVEITFKP"
     gene            complement(92567..93964)
                     /gene="argH"
                     /locus_tag="PLT63_00430"
     CDS             complement(92567..93964)
                     /gene="argH"
                     /locus_tag="PLT63_00430"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOD97214.1"
                     /translation="MEMKEKKSKSKKPWGGRFKKDTDRRVESFTSSLAYDRRLYPYDL
                     AGSVVHARMLARQGIISPAEAEEICRGLQAIGEEIESGSFRFDESDEDIHMAIEKALT
                     ARIGKAGEKLHTGRSRNDQVALDVRLYLREQTEALLKLVGDLKKRLVETAKKEIEIVM
                     PGYTHLQKAQPVLLAHYLLAFWEMFDRDEERFRDCRKRIDVMPLGSAALAGTSLSLDR
                     EYAAELLQFPRISRNSMDAVSDRDFIAEFIFAASLLMTHMSRFCEDLIIWSSQEFGYV
                     EISDAFTTGSSIMPQKKNPDVAELIRGKTGRVYGHLIAILTILKGLPMTYNRDLQEDK
                     EPLFDTVDTVTAAVAILTEMITRIVYLRPRMEAEAELGFTTATDLAEYLVEKGVPFRE
                     AHSIVGRIVAYCLDAGKELAMITFEELKKFHAVFADDVLPRLKAARSVNMKKTFGGTS
                     PERVAERIREIEQGE"
     gene            complement(94001..95266)
                     /locus_tag="PLT63_00435"
     CDS             complement(94001..95266)
                     /locus_tag="PLT63_00435"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOD97215.1"
                     /translation="MNKKKVKKVVLAYSGGLDTSVIVRWLLETYKCEVICFAADVGQK
                     EELDGLREKAVNTGAAKIYIDDLTEEFARDFIFPALRANAVYEGIYLLGTSLARPLIA
                     KRQVEIAHAEGADAVCHGATGKGNDQVRFEMTYMALDPHIRIISAWKDPHWKFDSRRS
                     MLEYAAKYNIPVPLVTKEKPYSSDRNSLHISHEGGILEDPWAEPPEDMFVMSVSPEAA
                     PDKPTYVEIDFEQGNPVAVNGRRLTPARLMEVMNELAGANGVGRVDMVENRFVGMKSR
                     GVYETPGGTALWIAHRAVESLTMDREVMLMRDSLIPKYAQLVYNGFWYAPEMKTLQAY
                     IDATQENVTGTARLKLYKGNVIVVGRKSPFSLYSEDFATFEKEEVYNQMDATGFIRLN
                     ALRLKIKAMQREGQDARKGKERRKGERRK"
     gene            complement(95263..96186)
                     /gene="argF"
                     /locus_tag="PLT63_00440"
     CDS             complement(95263..96186)
                     /gene="argF"
                     /locus_tag="PLT63_00440"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOD97216.1"
                     /translation="MNKDLVTEYDLAGEDFDFIFTRAGELKRFLKEGRPHQVLTGKTL
                     GMIFEKASTRTRLSFEVGMYQLGGLAVFLNTSDTQLGRGETVSDTAKIMSRYLDGIMI
                     RTFAQKTVEDLAAHADIPVINGLTDLLHPCQILSDLFTIMEKKGDYRGLKIAYIGDGN
                     NIANSWINAASRLPLKLALACPEGYEPDSELLARGRNETREGIYLCRDPEEAVEKADV
                     VYTDVWASMGQEAEREHRSRVFKTYQVNENLLSHAQEDYIVMHCLPAHRGEEITSGVI
                     DGPHSVVLDQAENRLHVQKAILETLMKEEKS"
     gene            complement(96183..97376)
                     /locus_tag="PLT63_00445"
     CDS             complement(96183..97376)
                     /locus_tag="PLT63_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOD97217.1"
                     /translation="MNEKEYISIADKCIMGTYRRYPVVFTRGRGMKLWDSEGREYLDF
                     VAGIAVCSLGHAHPAVTRAMQEQAERLVHVSNLYYIEAQIEYARLLTENCALKKVFFC
                     NSGAEANEGAIKLARKFGNDSSSGMRNEIISMIASFHGRTMATITATGQEKFQQGFHP
                     LLPGFKYAPFNDLETLAGMITERTCAVLVEPIQAEGGIRLPTPGYFAGLRKLCDERNI
                     LLILDEVQVGMGRTGSLFAYEQEGIIPDIITMAKAMGNGFPLGATLAGEKVAAAFEPG
                     SHASTFGGNPLGMAAARATLEILLSEGIIQECRNKGSYLMARMQELQARHKLIKEVRG
                     RGLLVGVELNREAAVIIARAMKRGLLIASAGPNVLRFVPPLIATKQDIDQATAILDEV
                     FKEEE"
     gene            complement(97469..98359)
                     /gene="argB"
                     /locus_tag="PLT63_00450"
     CDS             complement(97469..98359)
                     /gene="argB"
                     /locus_tag="PLT63_00450"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HOD97218.1"
                     /translation="MDTVKKSMERAEILMEALPYIRRFYNKVIVIKYGGHAMVDDELK
                     DMFARDVVMMKYVGIHPVVVHGGGPQIGGYLKKLGKESQFVQGMRVTDQETMDIVEMV
                     LVGKVNKEIVGLINQHGGRAVGLSGKDGRLIKADKYLLSAEKAKDTPPEIIDLGLVGK
                     VREVDGDLLTALVRDGFIPVIAPTGHGDQGETYNINADIVAGAVAAALKAEKLVLLTD
                     VAGVLDSDKRLINTMSNAEALGMINDGIIQGGMFPKVKCCLKALRGGVRKAHIIDGRH
                     KHAILLEMFTDRGIGTEIVI"
     gene            complement(98384..99817)
                     /gene="hslU"
                     /locus_tag="PLT63_00455"
     CDS             complement(98384..99817)
                     /gene="hslU"
                     /locus_tag="PLT63_00455"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HOD97219.1"
                     /translation="MINPKPLLKFSAPDNDPDLKALTPREIVEKLDQYIIGQGDAKRA
                     VAIALRNRWRRQNVSAELGEEISPKNIIMIGPTGVGKTEIARRLAKLDNAPFLKVEAS
                     KFTEVGYVGRDVESMIRDLVELAINMAKAEEQENVRAKAATLAEEALLDVLLPPKPVE
                     RSVGDMMSDDKEASFGETERLRKEDTTREKLRRFLHEGRLDGRYVEIEVSEQRSGPMI
                     EIFSTAGIEDMGMNIKELFGNMFPPKKKKCRVMVPEALEILTQEQAQTLVDMEKVTKT
                     AIRRVEQSGIIFLDEIDKIVGSDSGHGPDVSREGVQRDLLPIVEGSTVNTRHGMVKTD
                     HILFIAAGAFSASKPSDLIPELQGRFPIRVELDSLGKEEFIRILTEPHNALIKQYIEM
                     LATEGIQLSFTDEAVAEIAEVAAIVNDRTENIGARRLYTIMETLLEDISFDAPDLMEK
                     VVVIDAEQVREKLEDIIEDEDLSRYIL"
     gene            complement(99810..100355)
                     /gene="hslV"
                     /locus_tag="PLT63_00460"
     CDS             complement(99810..100355)
                     /gene="hslV"
                     /locus_tag="PLT63_00460"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007302170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HOD97220.1"
                     /translation="MKMRGTTIIAIKRNGMVTVAGDGQVTFDVTIMKHGARKVRRLYH
                     NKVIVGFAGTTADAFTLFDRFDQKLDQYNGNLLRAAVELTKDWRTDRVLRHLEALMIA
                     VSRDHFLLISGNGDVIESDDEVMAIGSGAPYALAAARALVRFSELSATEIAVESVRIA
                     SEICIYTNDNITVEELEIDND"
     gene            complement(100352..101311)
                     /locus_tag="PLT63_00465"
     CDS             complement(100352..101311)
                     /locus_tag="PLT63_00465"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HOD97221.1"
                     /translation="MQRDDQEKTLIKAGEDFIVHLEMERNLSPRTVKSYRSDLIGFFA
                     FLSEGENTEDYLATASVRSVDQLLIRGYLATLHRQRLKRTSIGRKMAALNTFFRFLLE
                     KGYVDVNPLAGMSAPRKDRYLPAHLSVDETHDLLTQEFPDDEKGRRDRALLELFYSTG
                     IRLRELTDLNREDIDFTQALIKVRGKGRRERIVPVGAPALSALRSYLDLQTENSRKGV
                     GGKSPLIRGQNDRRIHPRTVERILDKYIVSAGIAKKIGPHALRHSFATHLLDAGADLR
                     SIQELLGHKSLSTTQRYTAVNVSRLMEIYDQAHPQGRGGEEKQ"
     gene            complement(101320..101820)
                     /locus_tag="PLT63_00470"
     CDS             complement(101320..101820)
                     /locus_tag="PLT63_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HOD97222.1"
                     /translation="MFIRMKITGLTIDPITSTPIVILKDMEEKKAIPIWIGLFEASAI
                     ATQLEKITFSRPMTHDLIKDILKTMEVEVVRVEIHDLRNNTFFANIHLRGGGKEFVVD
                     ARPSDAIAVAIRTDSPIFVAELVIQKSRHVDIGANIEDMEKLKEEKLKEFLENLSPEA
                     FGKYKM"
     gene            complement(101835..103262)
                     /gene="miaB"
                     /locus_tag="PLT63_00475"
     CDS             complement(101835..103262)
                     /gene="miaB"
                     /locus_tag="PLT63_00475"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOD97223.1"
                     /translation="MGRENFADRRFFIHTFGCQMNVHDSEQLANIMTEAGYEQTPDVR
                     QAKIILVNTCSIRSKVAQKIVSQLGRYRRWKDKDPEVIIGVGGCLAQHWGRDMFTKTP
                     YVDLVFGTHAFHEVPQLVDMVRAGHGPVMDTELREEIPSLGICTAGKNGNGKTKHHSL
                     VTIMQGCDNFCTYCVVPYLRGRERSRPSAEIIREIEALVKQGIREITLLGQNVNSYGR
                     GLPEETNFPKLLAMINDISELERIRFTTSHPKDLTGGLVNCFGRLKKLCEHIHLPFQA
                     GSDRVLTRMNRGYTRKDYLERITALRQCCPDIAISADVMVGFPGETADDFDATLALME
                     EVRFDNLFSFMYSEREGTVAVSFGEKVPEKERNRRLKILQELQDRHTWERNQALVGKD
                     VDVLVDGASKNDPQELTGRTRTNKIVNFAGPPILRGKTVTLNITGAYLHSLRGELKSP
                     AAINVHKSPEVILGRKSPEVILVQK"
     gene            complement(103337..104251)
                     /locus_tag="PLT63_00480"
     CDS             complement(103337..104251)
                     /locus_tag="PLT63_00480"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOD97224.1"
                     /translation="MEKRVMPQQRQAYVYAFLTVLFWSTVATAFKITLRYLSALEMLL
                     YASFTSTLILGIVLVLQGKMPLIRRCTRRDYLHSLVLGFLNPFLYYLILFKAYDLLPA
                     QEAQPLNYTWPLMLVIMAGLTGRERIDLLRIMTMLVSFIGILIISTRGDLISFRVTNL
                     PGALLAMGSSIVWASYWLANLRDRREEGVKLFLNFFFGFLYTLTLSICLAKLGRPSWP
                     GLAGAAYIGIFEMGITFFVWMKALTLSRSSIQVTRLIYLSPFISLVFIHFIGGEAVRA
                     ASFFGLLFIVAGIVLQEWVGRRIKTANK"
     gene            104539..105861
                     /locus_tag="PLT63_00485"
     CDS             104539..105861
                     /locus_tag="PLT63_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOD97225.1"
                     /translation="METVDILITDGRVVTMDETDTIYENGAVAVAQGKIVAVGLTDEL
                     KKRFSGLRTISARNHIVMPGLINSHTHTAMTCFRGFADDMELMTWWKEYIFPAETRTV
                     SPELAYWGTTLACAEMIRSGTTTFCDMYFFMEETAQAARKAGMRCLIGEVLLDFPSAN
                     AKSPAEGLEYTRKLIEKWSGDPLVRIAVEPHSLYTCSENVLKEARALAVRYNVPVAIH
                     FLENQAEVKQLTEKYGKKAIHYLREIGFLHDNLILFHGVCMDDEDMLICADYGCKVVH
                     NPESNMKLASGVAPVGKMLARGITVALGTDGCASNNNLDMFQEMDTAAKLAKVAALDP
                     TILPAPLVVRMATRNGALTMGLEQAAGALEVGKRADIILIDIRKPHLTPLYNEYSHLV
                     YAVNGADVETVIIDGRPVMEDGRLLTIDEKEVMTRVNKIASQLRGSFS"
     gene            105937..107223
                     /locus_tag="PLT63_00490"
     CDS             105937..107223
                     /locus_tag="PLT63_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOD97226.1"
                     /translation="MSGGEEIITEPQLGIRDGRILFIAPAHDPLATLYAPQEVIDASG
                     CLILPGLVNTHTHVPMVCFRGLADDLPLMDWLNNYIFPAERMFVNREMVRAGALLGIA
                     EMILSGTTTFCDGYFYASTIARTAQESGVRCVAGMGILDADISLVEEAEVAAHVTAAE
                     KFLTKWAEARPTIIPALFCHSPYTCSPQTLRAVKEVARRHDSLFLIHLAETAEETDII
                     TARYGTGPVFHLESLGVLDARTVAIHCNWLNEEEIDCLAKNGVKVSHNPQSNMKLATG
                     VGPVPALIAAGVTVGLGTDGCASNNNHDMFGEMDGAAKLHKVFHKDPKVMAAPQVLKM
                     ATMDGARVLGLEDQIGSLAVGKRADIIIVNLQKPHLTPLYNPFSHLVYAASGADVRTS
                     IIDGKIVMKDRRILTMDVGAAMDAVADIASAIRKSR"
     gene            107275..108843
                     /locus_tag="PLT63_00495"
     CDS             107275..108843
                     /locus_tag="PLT63_00495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HOD97227.1"
                     /translation="MRKIFSLFVAGLVMIWVTNFLLTSYAIAAPLEVTLYPASAHITE
                     LQKLPLKAIDGKTSEAVFLIPGQSDPETLTTKIVNPANLKVLDQRWRQVTRQDEQAVT
                     LLRKELADLKKEHRRLQADIRSLETQIQFWQLQTKVRMKTVTEAGNMSAAIGKNMRKT
                     VQDKLALEPIIDDITKKIKDLEEKLQRTTGEKETSWEVTVVLSGAVVREATLAYGYTA
                     TGCGWSSMYRLEAQPLKHRVRFTWDAQIWQSTGQDWRQVSIRLATLQPRKIIEPPELP
                     PWIIKPRERLIYRNQKSARGAMLMEAADQAELASPEGGAPTLTRESSFHVWEIGKRSL
                     AAGQRQRLQVREEEWPVEFLHLTRPAQGEQTFVRGEVKFSEDREIPPGMALYIIDGSL
                     VGKRSFSLAGREGTIYFGSDPLVKAMAVLINKAAGEETLFNDKQTYRWGWRIEISNAR
                     SYPVRVRIEESLPQSRDERIQINLKSDPAPTETTPNTQIWIVEAPAGGKRHLLTTVSL
                     EAPKNLPLDLGWRR"
     gene            complement(108925..111627)
                     /locus_tag="PLT63_00500"
     CDS             complement(108925..111627)
                     /locus_tag="PLT63_00500"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOD97228.1"
                     /translation="MPLLTVYFQLHQPFRLHPDRDKFLWEKQNRDIFIKVAEKCYLPA
                     TYLFTELIRAYPGFKITMSMSGVFLEQAENYQPELIKALQGLVDAGQERGQLEFLDET
                     YYHSLTSLFKDLAKREFRDQVSLHRDKIRRLFGFFPSAFRNTELMYNNNIAAVVADMG
                     YRAILCEKRDDMFENGGAPISPNAVFRARGSSLVVLPRHRELSDDIAFRFPHRPLTPE
                     MYASFIARIDGEAVILGFDYEHIGEHIWEDKGIFAFWRELPAALALYPSIVMANPSEI
                     AEHFREVECPVVDIDGLATSSWADATRDTFGWLGNMTQYELFKDIENMEADARKAGGD
                     LLTRWRHLTTSDHVYFLHESIGEDHAVHAYFNPYGGSISQPTYILTRKIDQLMSDLAR
                     FEIIKRTEKTAVLIITPETGPLPEEMGDLAKYISGKSGGQGEVVSALCEGMTERGINV
                     HLVTLNLKRRFQRESNLDEWEWREQRYKLDPKRIHLVSSAIFADSMSAYAGNPLLTAA
                     EFQRDIVNNVIKTVRAQHQGKLIIHSHDWMAGGVIAAYAKETGLPVLHTVHNVFTAHL
                     PLEHLGGVGWQNYAQNLYLSFDHGRQCVDCQATAIKNATMINFVGERFLREVVNDYFM
                     DRPIIPSSVRQEVKAKYQYGAARAIINAPASNMYPENCPHLVRTYGVEDEILAAKEEN
                     CREFQRRLGLNIDPGAILFYWPSRLDPFQKGVELLEAIAQDFVDAYSDVQIAIVGDGV
                     GSDRTHVDILGRIAYTSGGRIAYHPFTESLSMLGYAAASDVFGASLYEPCGQIDQVGN
                     LFGATATNRDTGGYHDKIRELRLKEDGSPQDVGNGFLFRDYDRGGLWYALEKSVQFHR
                     KPRDVRERQLKRIMRQAREAYDLGTMIAEYILIYERLNGDQPLT"
     gene            111867..115724
                     /gene="hrpA"
                     /locus_tag="PLT63_00505"
     CDS             111867..115724
                     /gene="hrpA"
                     /locus_tag="PLT63_00505"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HOD97229.1"
                     /translation="MIKRHLKLQDLCEKRRILRPATRYPAQLPITACREEIVAAIRRH
                     QVLIVTGETGSGKSTQLPKMCLEAGRGIRGLIGCTQPRRVAAAAIARRIAEELGEELG
                     NSVAYKIRFDERSGPHPYIKVMTDGILLMEAQSDRLLRRYDTIIVDEAHERSLNIDLL
                     IGFLQTILPRRRDLKVIITSATLETEKFAQALGNAPVIKVSGRLFPVEVRWRPPVSED
                     GLDDGGAYADGAVTAVADLERNPREGRWGDILIFMPTEQDIRDTCAMLAGRFGDDKVV
                     VPMFSRLSWEDQQRVFRPEDRQKIVVATNVAETSLTIPNIRYVIDTGLARISQYNPRS
                     RTAGLPVTPISQSSADQRLGRCGRVGNGICIRLYSEDDYLSRPAFTLPEIKRANLAGV
                     VLRMLALNFGDIFTFPFLEPPDKKQLRDALDILRELGAVRMGEGPTLTEIGRFMARLP
                     VDPRMARMLVAAASHDCLEEMLVITSALSIVDPRERPRDQAPLAAEIHGRFHDPTSDF
                     ITFLNIWKRCQMVWEESGSQGRLRKFCRDHFLSWRRMREWQDLHRQLQTMTGEVLVEG
                     RQGEDHKAEVRPSRLLRHLPVRSQGPKRGREDKNASIHKAVLTGFLSGIAVRKEKNIY
                     TAPKGRTAMIFPGSGVFGTGADWIVAAEWVETSRLFARTVAVVQPEWIEGAAGELCRT
                     VYSEPYWNRERGEVQASARIVLFDMVIIPHRTVSFGRINPAAAREIFIREGIMTGSLK
                     KSYPFLSHNEQVLEGVRVMENKLRRRGWLWNEETVFEFYRRHLPEEICEERSLQRCIR
                     QRGSDEFLWMEQTDVLLAEPDENALREWPDAATVQGIPVPLIYNYNPGGEDDGVTARI
                     MAPLLAADETERLAVAIPGIRREIVREYLQSLPKSYRRRLPSLRDVTDTILMNLNDPD
                     RPLPEVLSRLLNRHYDLDLPITVWQETKRLGSGQIRYLLTDEEGRELSHSRSLPELRE
                     RYLEKIKKETLHNARKQWEIENIQSWDFNDLPESIPLGDAVLPVDVVYPALRDEDGII
                     CLRLLDSPKEALAVHRQGVAALYERHFAAECKYLKKVLLPAGNMKLHGEKFGGSKKVQ
                     RILYSKALLDLFFVNVRTNIDYLKHAAEVKSLIIPHAKVVLQLAAPVLEANFQAEIVL
                     DRLEKQYGFSKPHREHLTFLRRELACLIPEDFLNRYPAERIRTLSRYIRGLIIRAERA
                     MLNLDKALAKAGEVDVYREQWRQLTVGSQHSPSLAKMQAQEDFFWFLEEYKISLFAQE
                     LKTTVRVSPKRLEERLRELRAML"
     gene            115792..117720
                     /locus_tag="PLT63_00510"
     CDS             115792..117720
                     /locus_tag="PLT63_00510"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOD97230.1"
                     /translation="MSMSIISRLSIKSQGLQYKLIIIQGLVFVLPFCLLTYILYIKNA
                     FQDTEHVVAYLLILFVVLAGIVTLRQIFDRIFKFAQHVEQAALGYASTSTREDLDDSE
                     DLKEISHSFQTLLDKFQQVGAELTKKTGDLMAIKELTEMADHRLDLSRLMQSLTDKAL
                     EVATAAATAAYYFEKDQALRIIPASGSDLSEDYQKFSYDMECRARLVLLDMKASISPT
                     DAHTFSMISLPVVDEEDIKAILILVRRKNQKPFGSEDLDSLGIMLNAVKSTIKNALLY
                     RRSEDQRAMLKEKSEFLAVEIEKRKTVEDSLRQTKEKWRRYSFIVNTAKEFMTLINRE
                     YHYETVSQSYCHAHQKSPEEIIGKSVHEIWGPGSADLIVKNLDRCFTGEEIYYQEQFA
                     FNDGVSRYYDVNYYPYRSVDDGKITHAIVITHNVNDYMVNKIDLENTVTKLRELMKGI
                     IATISNLIEMKDPYTAGHQQSVANIAYAIATAMNLPQEQKELVRISAEIHDIGKISIP
                     AEILSKPSKLNEMEWALIKAHPEMSELILSKIDFPIPIAPVIVQHHERIDGSGYPRGL
                     KGDEICLEAKIIAVADVVDSMSSHRPYRPAVGQEEALEELKRNRGILYDAAVVDAYMS
                     LDSTAINPDSRPDRCIPG"
     gene            complement(117807..118490)
                     /locus_tag="PLT63_00515"
     CDS             complement(117807..118490)
                     /locus_tag="PLT63_00515"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOD97231.1"
                     /translation="MGAAETIAPGIYLVGGPNISRSEDATVFVIDGGDELVMIDAGAG
                     RSARILESNILDAGLDPQKISLLILTHCHIDHIGAAAYFQREFACRIAIHADDAAAVE
                     TGDPIMTAANWYEMDFPPLSVDEKLTGAKGVYRCGPHELHWLHTPGHTPGSICLYLDR
                     DRKRILFGQDIHGPFLPAFRSDVRQWRESMEKLLELNADILCEGHFGIFQPRAAVEKY
                     IRRYLRQYS"
     gene            complement(118506..119417)
                     /locus_tag="PLT63_00520"
     CDS             complement(118506..119417)
                     /locus_tag="PLT63_00520"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOD97232.1"
                     /translation="MNLSMDVEIGSLRLKNPVMTASGTFGYGEEYAPYVDLNSLGAIV
                     VKGLSWKARSGNPSPRIMETPAGMLNAIGLQNIGVKYFINKKLPFLRNFDIAVIANVY
                     GETVEEYLRVSELLTAAPGVHALEINVSCPNVKKGGVAFGTDPDMVADVTGRVRGITD
                     LPLIVKLSPNVTDITVMAAAAAAAGADGVSLINTLTGMSVDVERRIPHLQNITGGLSG
                     PAIKPVALRMVWQAARTVNIPIIGVGGIMTAADALEFIIAGATAIQVGTANFINPKAT
                     MEILAGIRDYMDRHKINIRELVGTLHT"
     gene            complement(119465..120271)
                     /locus_tag="PLT63_00525"
     CDS             complement(119465..120271)
                     /locus_tag="PLT63_00525"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HOD97233.1"
                     /translation="MYTAYVIYNHRLNERDFHLALRLPKEFVQPRPGQFVMVRTAESD
                     GPLLSRPLSVYHFTRLHGHVVLELLYRIVGRGTALFSRWPSGKEVRVLGPLGNGFHID
                     EKKRNHILIAGGMGIAPITYLAAWLCNRSSDGNRMVFCLGAKNSGEFIGLERLEKIGG
                     QFHISTDDGSLGTCGTVTELLPEIVGRCEADAAVLYACGPPGMMKSLASCLPAKVTCQ
                     VSVEARMACGVGACLGCAISVLASRKRIVKKSVCRDGPVFDLREICWEDL"
     gene            complement(120275..120775)
                     /gene="rimI"
                     /locus_tag="PLT63_00530"
     CDS             complement(120275..120775)
                     /gene="rimI"
                     /locus_tag="PLT63_00530"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HOD97234.1"
                     /translation="MAFEIRIRPMETGDIEEVEAMEHCCFPEPYTAATWRQERQNTIS
                     RTFVALPGKDDGGNILVGYINFWIVIDEAQLHRLAVKEDFRRRGIAAGLIRHMLRYLS
                     ERSVSTVQLEVRQTNIQAVRLYENFGFVTWGRRRGYYRETGEDALLLTGAVNDILNVA
                     EAERGG"
     gene            complement(120765..121460)
                     /locus_tag="PLT63_00535"
     CDS             complement(120765..121460)
                     /locus_tag="PLT63_00535"
                     /inference="COORDINATES: protein motif:HMM:NF015566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HOD97235.1"
                     /translation="MLLEMTAVSILGGFLCLDRTCLQIMVSRPVVVAPLFGWVLGDVL
                     TGLTIGAFLELLWVDKQQIGIYVPPNDSLLAIVIVTALLLAFPQLTERRQELTAFAFL
                     ALLPMSYLTKRMDTALIQANEALSDNALRAASSGDIETIGREHRRAIYRTLTSYVIFL
                     FLASGVGILTLRAIFPVLPENILQALRLMHGIIPLVLIAVCLNSINFRRLVPLFCAVV
                     LTTMIIIELVNGF"
     gene            complement(121483..121968)
                     /locus_tag="PLT63_00540"
     CDS             complement(121483..121968)
                     /locus_tag="PLT63_00540"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HOD97236.1"
                     /translation="MNCVLVRVDNRLVHGQIIEAWVPHLKIAQIIVVDDEVASDFFRE
                     TVIKMAVPSEMVVKIKSVEDFVREALGEAPGGARSIVLFSRVWDALRAYALGFVFEKI
                     NIGNIYNDDCRQRLSACVLLNDEDLDSIRSLLQRGVNIELRRVPRERPVNIAEVLKEI
                     L"
     gene            complement(121973..122383)
                     /locus_tag="PLT63_00545"
     CDS             complement(121973..122383)
                     /locus_tag="PLT63_00545"
                     /inference="COORDINATES: protein motif:HMM:NF015567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOD97237.1"
                     /translation="MIGVLITTHGELGHELIKAAELIKGSLKGVAHISVDQTKGMEEI
                     KKEISTAIKKLDQGKGVIILTDLFGGTPSNISLSFLKEGKVEVITGVNLPMLLKLSDG
                     RSNMNLREFANFIKEYGIKNIYLASEILNKKIGA"
     gene            122778..123659
                     /locus_tag="PLT63_00550"
     CDS             122778..123659
                     /locus_tag="PLT63_00550"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOD97238.1"
                     /translation="MAVVALSGIWGYNWVVMKECLHYASPFDFAALRTSIGAASLFLI
                     MFIQGKSLYPKEFILTLALGLLSTTGCIGLVTLALYYGAVGKTAILVYIMPFWVLILA
                     WPLLAERLAGSQWMAVSLAFAGLMVILEPWNLRSGMVGNILAVSSGIAWAGSAVLAKI
                     IWRRYPLELISLSAWQMLLGSLPLIIMAITIPGPPVQWTPYFIVGLSFSSVISQALAL
                     ILWFYLLKALPAGMASMGTLATPIIGMIAAAIELGERPSLFEGIGILLILAGLIILAL
                     QGLIHARELRAIIAGQK"
     gene            123685..124794
                     /locus_tag="PLT63_00555"
     CDS             123685..124794
                     /locus_tag="PLT63_00555"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HOD97239.1"
                     /translation="MERNLLDNIEEILLMGPGPSCVPPAVYRALARPTLGHLDPYFLT
                     IMDELKILLQQIMNTANNLTVPVSGTGSAGMEACFVNLVEPGDRVLILINGVFGMRMR
                     EVASRLGADVEVMEFPWGTPVNPSMVGEKLWEGNYRLLAVVHAETSTGVANPVAEIGA
                     LPRGDDCLYLVDAVTSLGGMEVRMDAWGIDALYSGTQKCLSCPPGLAPLSFSPRAVAK
                     LNGRRRKVPNWYLDLSMIIDYWGKNRVYHHTAPVNMLYALYQALQTIFAEGLDAVFTR
                     HRRTHAMLVRGFGELGLKMVVEEPFRLPMLNTVYCPAGIDEAAVRRALRSEHRIEIGG
                     GLGPLTGKIWRIGLMGHTAAEENVRRFLDALRTVI"
     gene            complement(124799..125518)
                     /locus_tag="PLT63_00560"
     CDS             complement(124799..125518)
                     /locus_tag="PLT63_00560"
                     /inference="COORDINATES: protein motif:HMM:NF024872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOD97240.1"
                     /translation="MTMLKKDLILRNPLRQMGYEDDDIIPAGGFGAVLARAGVGKTAL
                     LVQFALNTMLRGKRVLHVGLHDPVNKVDLWYRELFRHLAVQYQVGQIRTLWEEILPLR
                     FIMTFKVEGFSVPRLRERLNDLTAQGIFYPDTIIIDGLIFDDFPQSALADMKSMIEEL
                     SLRAWFTVHVHRQVQADADGIPKPLLPADDLFDVALLLVTEGNEMHIRDLKSERTPSP
                     HPVLILDPATMLIKENVAVAS"
     gene            complement(125563..127650)
                     /gene="fusA"
                     /locus_tag="PLT63_00565"
     CDS             complement(125563..127650)
                     /gene="fusA"
                     /locus_tag="PLT63_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOD97241.1"
                     /translation="MKRDLAKIRNIGISAHIDSGKTTLTERILFYTKRIHAIHEVKGK
                     DGVGATMDSMELEKERGITIASAATYCEWNDHEINIIDTPGHVDFTIEVERSLRVLDG
                     AVLVLCSVGGVQSQSITVDQQMKRYRVPCIAFINKCDRSGANPKRVVEQLRGKLGHNA
                     VLLQMPIGLESNFHGVVDLIEMRAVYFDGPHGEKLRYEEIPAVLLPEALRLREEMIDQ
                     ASIYSDDLTEAILEEREITTAMIFTALRFGTLKRRITPVVIGSAYKNKGVQKLLDTVT
                     SLLPCPAEVENTALNLDENEAPVILKNSPDEPIVALAFKLEDGPFGQLTYIRVYQGTI
                     AKGTTIVNVRSGKKIKVGRLARMHADQMEDIDTIPAGYIGALFGVECASGDTFVSPGL
                     NLAMTSMYVPAPVISLAIAPKDNKAQINMSKALSRFTKEDPTFKCHVDRETNETIIAG
                     MGELHLDVYVERMRREYGAEVTTGNPRVAYRETITQRADFNYIHKKQTGGAGQYGRIA
                     GYMEPTSEGDFIFENDIVGGAIPTQYIPACEKGFRQSLEKGPKLEFPITGIRVVINDG
                     AYHAVDSSDMAFQAAARGAFRDAYLRARPVVHEPIMKVVIESPTEFQGSVMALLNQRR
                     GMIVGAQDEGVMCIVEAQVPLAEMFGFSTVLRSATQGKAQFTMEFSLYRQVPNSIAEK
                     IAEEIAARKKNAA"
     gene            127845..129122
                     /locus_tag="PLT63_00570"
     CDS             127845..129122
                     /locus_tag="PLT63_00570"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOD97242.1"
                     /translation="MNFTVHTSSDYHRRELTSLGWETTLCNTIADPHGPCRTVLGIDA
                     AYGELLGDYLRRYVPFPDISHLVEVGGGYGWLMRDLLRRHHFPKVTMIDICPTLLAVQ
                     KETLGRIRGVIDFREEDFLDTPHEMLRGADLVILNENVGDYPTITDLTRESLENREAA
                     PAPELRAVSALFHRYGLPVPDQARFHVNIGALLTLEKLCRAEVPYIFLSEHSCEASVS
                     IPYRDLIHVPASGNPECIVLKGHEEFTIRFSHLEKLAESYGYRTIRGPIADFIPFHVT
                     PRVRAILKAPSPWRDEDEIVRYFLEDLFKYEYLLLAKETETEHAQDGGDLTCRRCGKC
                     CLADFAAYVKDEDLERWRREGREDILAIIAREHAVWAGDHLISAADGRYLHGCPFLTW
                     EGVHTTCAIYETRPAVCRNFKPASVEICPLWRP"
     gene            129216..129509
                     /locus_tag="PLT63_00575"
     CDS             129216..129509
                     /locus_tag="PLT63_00575"
                     /inference="COORDINATES: protein motif:HMM:NF019491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HOD97243.1"
                     /translation="MLRHIVLMKFKEGVGERQIEEMSAALRSLPPKISVIKGFEFGPD
                     ILRTDRSYDFALVASFENVDGLKAYQLHPDHVPVLQLVRSLCDALIVADFTWP"
     gene            129536..129787
                     /locus_tag="PLT63_00580"
     CDS             129536..129787
                     /locus_tag="PLT63_00580"
                     /inference="COORDINATES: protein motif:HMM:NF012675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HOD97244.1"
                     /translation="MPTPVKLFSLSTCSHCKATKKFLTDCNIAYDFTDVDRLSGDERQ
                     LAIDEVMKYNPHRSFPTIIIGDRIIIGFNEKAIREALGI"
     gene            129787..130119
                     /locus_tag="PLT63_00585"
     CDS             129787..130119
                     /locus_tag="PLT63_00585"
                     /inference="COORDINATES: protein motif:HMM:NF014939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-thioredoxin reductase catalytic
                     domain-containing protein"
                     /protein_id="HOD97245.1"
                     /translation="MDAARLYETLKKIQEPKGFLFNRDKNWVMEILNDLLVNKERYGY
                     MTCPCRLASGNREQDRDIVCPCAYREADVKEYGSCYCSLYVSREWNEERIEHLYVPER
                     RPPEKMGI"
     gene            130122..131120
                     /locus_tag="PLT63_00590"
     CDS             130122..131120
                     /locus_tag="PLT63_00590"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HOD97246.1"
                     /translation="MSGNKPILVSGNLPAAVLDFLREDFQVEYHAEDRPMPRRKLLRF
                     LDDKVGLLAMITDHIDEELFDHAPRLAMVANMAVGYNNIDVVAATRRGILVSNTPGVL
                     TETTADLAFTLLLAAARRVVEGDRMVRSGRFKFWAPFHFLGREVTGKTLGIVGMGRIG
                     QAVARRARGFNMPVFYHSRTPLRKSVEKNLRATFLPLTSLLKEADFVSLHVPLSEETT
                     HLIGRDELALMKPTAFLINTSRGPVVDEQALLSALREGIIAGAGIDVYEHEPVIATGL
                     EKIDQAVLLPHVGSATLETRTRMAWMAAENLRMGLRGERPPNLVNPQLMKKRKRGV"
     gene            131120..131680
                     /gene="rfbC"
                     /locus_tag="PLT63_00595"
     CDS             131120..131680
                     /gene="rfbC"
                     /locus_tag="PLT63_00595"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HOD97247.1"
                     /translation="MPFSFYPLDIPEVIGIDYRRFADDRGYFAETYKRSDFVAAGIAE
                     DFVQDNYSYSRRGAVRGLHYQKNPMAQGKLVMVAAGTIFDVAVDIRRGSPSFGHWVGR
                     ELSTQNGAMLYIPPGFAHGFSVLSNEAVVVYKVTREYAAELDRGIIFSDPELGIPWSV
                     PDPLVSPKDAAWPPLRKADIDYNYGR"
     gene            131713..132495
                     /locus_tag="PLT63_00600"
     CDS             131713..132495
                     /locus_tag="PLT63_00600"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOD97248.1"
                     /translation="MENRIQGKRILITGASAGIGKACAERFAAAGANLLLIARRLGRI
                     KELQAELSRENSVGIDCFSLDVRDREAVRKFAAELTDRKLTPDILINNAGLASGLNSL
                     QEGDWDDWDRMIDTNIKGLLNVSRCLIPLMIAAGRGHIVNVGSLAGYQVYPKGNVYNA
                     TKFAVRALSEGMNIDLAGTGIRVSMISPGAVNTEFSEVRFHGDREAAARVYEGYTPLY
                     AEDVAEAVYFIVNAPPHVNVQNMLITPTAQRNVYCVDRADKS"
     gene            complement(132756..133922)
                     /locus_tag="PLT63_00605"
     CDS             complement(132756..133922)
                     /locus_tag="PLT63_00605"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOD97249.1"
                     /translation="MQNFIFENPTKIIFGQGQIRRIGAEAARFGGKILLVYGQGSIKK
                     NGVYEQVMASLKENGLTVVEFSGVRSNPVLSHAVRGIEIAKREGIEAVLAVGGGSAID
                     TAKTIAAGARTDHDVWDFFVYRQTIQEALPVLTVVTVSASASEMNAAAVITKEEGRRK
                     YSIRSLHVQPKVSVLDPTVLFSLDKKYSAYSAVDAITHMLEGYFNNTEPAFSRLQDRL
                     VEGLMKTIMEATEKILVAPDDYNARAEMMWAATLAFNGLTTAGMGQVALPVHMIEHSL
                     SALYDVAHGAGLSIVLPGWMSRQVREKAGRFARLAREVFGINTADNDEAARAGINVLR
                     KWFAAIGSPVSLAEAGIPAGDIDRIAENAAELAQVWGLKAYSRQEIGAVLELCR"
     gene            134085..136115
                     /locus_tag="PLT63_00610"
     CDS             134085..136115
                     /locus_tag="PLT63_00610"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOD97250.1"
                     /translation="MKAKNHLDALFKPRSVALIGASGTPGKLGCDILYNLIHAGFQGP
                     IYPINPKADELLGLKAYPDIEATPAPADLAVIVIPSRMVTGTIEKCGRSGVRAAVIVT
                     GGFAEAGVEGEKLQQELAAAAARYGIRVVGPNCQGINYPHHNLCASWPLLTNRGGMAF
                     ISQSGTVGAALMDWASEENLGVSVFVSLGNRADVDESDCIRYFDKDSHTRVIAIYMEG
                     VKRPQYFLESLAKAKKPVVILKAGRTARGKVAAESHTKSLAGSDEIYNAIFSKYRVHR
                     ADNLEELYDFAKALAYMKKPKGRRLLNISSSGGAAILAIDEAEKLGFKNPAPGKNLKE
                     KLRSFLPAHCGVSNPIDLTGDAISDPSLYSRVIDAAKQDFDTSVVIFGDPIKGAADIV
                     SGEGELIVFCGGADVEREESRRMHAKGIPVYPTPERGIRALHQLVCFDPPRSVKKKKD
                     DAKPEHQGRQPLSLDTAVALMEKYGLPFVATRPAATAAAAATLAKAMGGPVALKISSP
                     DAAHKTDVGGVRLGVTGAAAVRKAYEEIQANVRTALPEARIDGITVSPMAPPGGLETI
                     VGVIRDPQYGHALMFGLGGIFTEIYRDVRFCLLPATERELLAMIRGIKGYPLLAGVRG
                     QKPRDEQALVALMRSLARMVQDHGDIDQIDLNPVLVYEQGALAVDFRIYGSI"
     gene            136187..136852
                     /locus_tag="PLT63_00615"
     CDS             136187..136852
                     /locus_tag="PLT63_00615"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOD97251.1"
                     /translation="MKIADGLYSYEWTNYYENNCNSFFIGGGVGAVIDPGLKSYVPDL
                     LQRMAADGIDGKDIRYVINTHSHPDHFEGSEYFHGRDISIGLHEDELSFLAGVGGELY
                     GLFGLPRPSVAVDLALSEGTAEFGGEEFQIFHVPGHSPGSIALYWPRAKALFPGDVIF
                     NQNVGRTDFPGGSGALLKQSIRSLSGLEVEWLLPGHMEIIGGTENVQRNFQTVIRHIF
                     PYI"
     gene            136867..137700
                     /locus_tag="PLT63_00620"
     CDS             136867..137700
                     /locus_tag="PLT63_00620"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: protein motif:HMM:NF003742.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HOD97252.1"
                     /translation="MLTSRRTVFFISDSTAITAEALGQSLLTQFDHVLFEQVTLPFID
                     TQAKAERVVNQINMAARQSGLPPLVFSTLINHDIRATVMKSQGSFFDLFETFIGRLER
                     ELSMESSHTIGRYHSFISNSIYDVRIDAVNYALGNDDGMSTKNYDKADIIIVGVSRTG
                     KTPTCLYLGMQFGIYAANYPLTDEDLFGRYDELPGVLIHFRKKLYGLTINPERLHRIR
                     TERLPASRYSSLEQCRSEIAAAESLFLSEHIPHINITSASVEEIAAMILHECGLQRRV
                     L"
     gene            137770..139608
                     /locus_tag="PLT63_00625"
     CDS             137770..139608
                     /locus_tag="PLT63_00625"
                     /inference="COORDINATES: protein motif:HMM:NF017078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE4 domain-containing protein"
                     /protein_id="HOD97253.1"
                     /translation="MTLRQKIYLVVALTFFALTMVMAFCSYTILIKGFEDIEREDVRS
                     NLYGALALLDQELASMDATARDYSSWDDMYDYIGTRRESFIASNYSQSGITNLKLNFL
                     IINDLQGNIVYSKGFNPETGHTVPILRSILAHRNLIHKHVFSGDEIKHLSGIISLPEG
                     LTLVIARPILKTSGKGTPRGTYIVGRFLDAREIDQMGAPLKSEVSIYHEGQKDLPAEI
                     ASVRKALSPHHRELIKAINGHTIAGYVLIKDAFGKYSLILKTIDKRVIFIQGMKTIAY
                     VIVFVLLTALVFASVMVALLEKSVLRRMLHISHDIRAIEADGDMSRRVHLGGTDEIAN
                     LGNDINNMLGKLEESEQRIRGEAERYRAVIEDQTEFICRFGADGVLSFVNDAYYRFFS
                     HRRQEMIGRHFLSFVPDEDRRLIYELLGNLTPSSATATYDSRVIFPEGQEFWQHWTYR
                     AIYDASGCLREYQAVGRDITDRKHAEEKLSRLNRQLEEANAQLAQAYANMKQNLDTLR
                     KHLFKEEFAFLVDREGQILGITERVLEYMKLSRNQMIEANVTDYLATHDRGKFIEALR
                     DAWIGITRQANFKLIRPQDEPSSQHFEIKFARLTLEGKRLLLVTLR"
     gene            139666..>140070
                     /locus_tag="PLT63_00630"
     CDS             139666..>140070
                     /locus_tag="PLT63_00630"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97254.1"
                     /translation="MTILFICTANIVRSYMAERILKGMLAKMKRRDVIVTSAGMLDMH
                     GAPGDPTAAKILAEHNFDGYGHISRPYSEALLSQADMIVVMEKAQEERLTADHPRYGE
                     RLRLLKTFSREYDGSAGDIKDPYRLSPYHYRLC"
BASE COUNT        31697 a        38149 c        38320 g        31904 t
ORIGIN      
        1 acgcttttaa agacttacct ccttccatcg ttacatatct ccgggaactc aggatataac
       61 ttaaaggaat aattattgga atatcggaga atcatgaaaa gaaaagacct tcccctgatc
      121 aagacgaatc cctatctgca agatccatcg gaatgcgatg cctggctgaa ccgtgccgtg
      181 atttcttcct ccgctgtcga gggtgcccgt gctgccgcct gccgagccct gggggtatag
      241 ggaagcgacg tggaatcaag agaaatcgca ccagtcccaa gtcctcctga tcaagccaac
      301 tactatcctg cccgatcgat ctttcttggg gggttagggg gggcacaaac tcattctgaa
      361 tattgttagg gcatttgggg tgaccgatca cccctgttgc gccagccact ggcgaccctt
      421 gtcggtcaaa cgatatttct gtaggcggct gctgggcctg tcggggatgg tcatttcaat
      481 caggccgtcg tccaaggccg gtttgaggta gcgctcacgg aacgatttgc ggtcggaaag
      541 gtgcagagcg gattgcagcg cctcccggcc catctcgccc tggatggccc ccagcagttc
      601 gccgacttgg ggggtgactt ggggggcgat agcggcccgc ctcgtcgagc aggccggaca
      661 tcatcagccg gtgcgcctcc agcaggtgct tttccgtctc gggcttccag acgccgaacc
      721 ggtcgtaggc ggccagggcg tttttcacct cctgcacctc gcggggcggg gcgataaccc
      781 gcttgccttc caagagcgcg gtgatctgcg cctcgttcag ggtgttgccc tcgatggcca
      841 gcgagccgcg aatggtccgg atgcggttga tgcgccgcag acgcagggcc ctggcctgat
      901 cggtgagcac ggtcagccgc cccatgccct tcgctgattg cggcaacccg gttcaggatc
      961 tccggggtaa tggtgtgggg cgtctgatag ctggatttcg tcataccggc ctcacaggta
     1021 cttttccatc cctgcctctt ttcataaagc tactttggac caggagctta cgcactttgt
     1081 ctatcatgcc tcaccgtctg actgtcaaac atgattgggt ttgatccccg gtcctcttga
     1141 cgttaccaga ttctttttca ggtgtatagc agggatcaac cgaactggta gcccatctgc
     1201 acccaataga gccagtcttc gacagcctgt ctggtttgct cgtcgaccgc agcgcccacg
     1261 gcaagttggg atagtggtac cgccatcctg cctttgcctc ggcggatcat gacgaacatc
     1321 tctgtttcgc atgcctcttc gggtgccatc ttgaggactt tgacctcttc ttgaagccgg
     1381 aggggcgagg tcgcgcgctt tgtccttcaa agggcggcaa agggaaaccg cagttgctct
     1441 tcaaggtagt aataccatga cattgcttgt tcttcgggtc cgtaggcatc gacgatgatc
     1501 accatggcga tgcgctcgtc ccgttcatca tcgggtttgg gttttttctt ggccgtatcg
     1561 tatcttaaaa gaagctatgc tgagcggacg gacgccctcg gccagcgctc agctagtgat
     1621 cggccgcact atgagtcgag gggtcgggaa gaatcctcag cgcctccttc ttcctcggca
     1681 atcagggaat cgatgatctt gcgcttggca gtctccggtc cgaacaattc ccgcaaggct
     1741 tcataggcct cgtagccgtt gggactcgtg gaatagtaaa aatctttggc cagtccggta
     1801 aaggtgtcca ggttgcactg ctccatacag gcctgggtgt aatggtcgat gagcagcctc
     1861 aggcagtttt cgaactcgtg aaagaaggcc agatcggaaa ggagatcgat ccttccggga
     1921 tggtgctcca ggccatacag cagcagggct ttgagatgct tgttggatgc cttgtagtat
     1981 ctcctgatca agggataatt gggcttctct gcggcctttt cctcggagta gccggcaaag
     2041 atctcctcgg agaggggctt ccggccttca aaataggcga cgagatcccg ctggcgtcgg
     2101 ttttccggcc gggcgttccg ttcttccttt gcttcgtcct cggtatcgtc ttcacagtat
     2161 gtaaacccga gcttctccat ttcggcgacg ggatcgcggg gatgggcgcg aaagatctcc
     2221 cttaccttat cgcggatgag ctcggattga agcctgtcgt agtctgcaag gacaggacca
     2281 ttgtcttggg caacgggctt gaatttttcg agatagatca cgttatttgg cttctgaccc
     2341 tggtcggagg gcttccctcc ttgccgaaac agttcctgga aaaggtcttt cggagaacgg
     2401 tatttcagat aggacatcgg cgtttcgttc aggtaataag aaacagcgaa gggtgtctgt
     2461 tgtccctcgc ccaggcggat cttggactgg aaaatgagat cgttcatgga gcccagtact
     2521 cgccgatcgg tagtcttggc aaagatggca tcctggaccg gcgaaagcaa ggtcatgacc
     2581 gcattgggct gatatccctg cccggtcaga aagtcttgca ggccgtcgct gagcgcctga
     2641 gcgaactgcc tgcccattgt gatcccccgt ccgagcatga ccagggagta gagagatgcg
     2701 gtgttggtcg ctaagatgta atgcgttcgc tgtacagtga agagatgggc gtgccaatcc
     2761 agcaggggat ttctgccttt atcgtaaggg agggcgggca ggggatcgag cccgatcttt
     2821 tgcgccaatt ttgccgtgag tcgaagaatc ataagtgttt atgtactcat tcctgtaatt
     2881 ctggaagtgc tttcattgaa tttgataacc ccattcacca taggcaaaca ggtcccttat
     2941 gccgctgcat agatgctgcg gccggcgccg tagtaggcgt catagacgtg ctggaagacg
     3001 gccgcggttt tctgctcgaa gagctccggc gtgtaggccc gcggcaagcc tttgtccagg
     3061 agcttttcga tggtgacgcg cacctcggcc cgggcctgtt gccgtttgcg ccaatcgagc
     3121 accagctttc ccgccttcag cgcggccagc aactcccggt ccgtggcctt gaccttctcc
     3181 cggtccgcgt cgctacgctc cgaccagggg tggcggaata aatcggaatc ccctggcgct
     3241 ttgcatcgga acagggtggc ggcttccttc ggaagcgagt ggcggaatca gcggaatacc
     3301 caccataggc ggcaaaaacc ccaacctggc agttctccac tccaattgac tcatctggcc
     3361 cgccatatac tgaacaaagt tttctcgagg ttcctcccga gaaaaacaat ccgcaaactg
     3421 ggcatgaaaa cctctgaact catcagtgaa ctggtcaatg tcctctttgc caacgaacaa
     3481 tttggggatg ggaaaggtcc catcctgaat gcggttttcc ggtaccattt tctgacacct
     3541 cccaatgctt ttataaaaat gtagcatagt cggaatgtgg tgtcaactta taaatagctt
     3601 atgtatcgtt gtggaactaa atggtgttct taaatcctta accagacagc cgttatatta
     3661 cgtatataat cctgaaatgt ccttgacatt tagtaaacat gctttaataa ttatgctatt
     3721 atcctcgaaa aggaggtgtc catggccaca aaatatatca aaggaaagct ctataaactg
     3781 aagatcgccg atctccagcc ggacccgcag caggcaagga cattcatgga caccgtggcc
     3841 ttgaatgagt tggccgcctc aatcgagaaa tacggcatcc tggttcccat tcagttttgt
     3901 ttgaatgagg agggggggct gatgatcgtc tccggccacc gccgcgtaaa ggcggcggcc
     3961 aaagctggac tgacggagat tcacggcacc ttcaccgaag gcgatatacg cctccaggga
     4021 tttgtcgata acctccagcg ggaagggttg acccccgtcg atgaagcgga gcagatgaaa
     4081 ctgatgatga aggaatatgc gttcaatcag tatcaacttg ccgacgcctt aggcaaggat
     4141 cggaccaccg tcaccaagac cctaagcatc aacaagctcc ccgaggacat ccgcagcgct
     4201 gctcggacga atccaaacat ccccaagagc gtccttttgg atgcggcccg gatgagcacg
     4261 gagaagagca tgcgccgtaa gttcctgagc tacatgaccc aggcggccaa ggccggacag
     4321 acctccgctc ggaagcagcg gttgtccaag gagcgcatcc tgattacgaa gacggacaat
     4381 ctgacggggg agctggacgg tttgccttgg cgggaatggg cggaggatga ccggaacgat
     4441 ctggccagcg ccttggcggg aatccgccgc aaagccgacg agctgctggc cgaaatggaa
     4501 tggaaccgcg aggaggagga ggaagagaaa ccttccgcca gcggcatgaa cctctcctga
     4561 aggtaaagta agaagaaacg acatctcggc accttttctc cggcggggtc ggcgacatgc
     4621 acatgccgga acgcgtaccg tgtaactgtt ggatattgaa cctaaggttt gaaaagccgc
     4681 tccggcccat tttatcggat atattaaaat tatatcaaac agttaagcat ggtgacgcga
     4741 tcacctcggt tgctaagaaa aggaaagggt taaatggcat tctactccgt agctgttcat
     4801 tatgcggcga aagcgcttca tccttcgtca ccacgacgca cccccaagta cttctcattc
     4861 ctcgggattc gcgcgccttg cctctggata tttttacttc gtcatcaaaa tgatgaatta
     4921 ctatgaaaat agagcaaata acgcaataat ataaacaaat acatattgtt atgcttcgtg
     4981 gcgctccaca gggcatgcgg gttttttaca agcccatcat taaatgggta gacaagattc
     5041 aagctacccg gagcagaata gccgtacgac ataaacaatg tcatcgccaa gggacgatca
     5101 acgggagaga tgaagtacat atcgataatc atggcgtttc ttttgatggg atgtgtcgga
     5161 atccctgaaa atgtaaaacc cgtcgagaat ttcaagtcgg agaaatattt ggggcgatgg
     5221 tacgaaatcg ccaggctgga tcattctttt gagcgcggtt tgaccagggt cgctgcggac
     5281 tacagcctgc tagccaacgg cggggtcaga gttataaacc gcggttattc tatcaaggat
     5341 aaaacctgga aagaaattga aggtaaggcc tatttcgttg aacggtcaga tcaaggatac
     5401 ctgaaagtat cttttttttg tcctttttat ggctcgtaca tcgtatttga actcgaccat
     5461 gagcattatc aatatgccct tgtctgtggc ccggataaat catatttgtg gattctggcc
     5521 agagccccgg aaataaacag ggatacattg gcggtcctca cggcgaaagc ggcaaaatta
     5581 ggttttgaca ccggaaaatt gatatttgtc gatcaccgct gatcgccggt aattgttcat
     5641 cccccaccat atagtttttt taacttttgg aacgggattt ttcctcgaga tgcatcggcg
     5701 tatcgtaaag catgcgccgg atggtttcac gatgatcggc cgccaagttg caggccattt
     5761 catcgcctgc cgtcaccact tccgccaggg cgacgccgac ttcagcttct ccggcgacga
     5821 ctaccgttgc gcccgccttt cgaagcgagc ccgcttcccg cagatgggcg cagcgcacca
     5881 gaatcttcaa atccggattc acctggcggg catgttttac gacttcagcg gcatcatcta
     5941 tatcggtgct caaaatcagg ctggtggcta cggcaatgcc tgcgtcttcg agcgttcccg
     6001 aacgaaggac gtcaccatac agggcattat ggccttcggc gcgcaattgg cgaatggtgt
     6061 ccagattgag atcgataacg gtgatttccg cgccacgatc gagaagcagc ctgtgcacga
     6121 tttttccgac ggggccgtaa ccgacgagga tgcataagtc gggattgagg ggctgacggg
     6181 ggatttccac aaccggcgtc gttttggtct ttttggctgt cgaaaagcgg cgcgcccaat
     6241 cataaatata aggattaaaa ccgatggata tgatggaagt agccaccagc gcgttccatc
     6301 cggattcatc aaggagaccc agaccaaggg caaccgtccc cagaatgaaa ctgaattcgc
     6361 cgacctggga aaaagccgcc cctatgggaa ttgccgtagc cgcgggctga cccaacattc
     6421 tgacggtgat cgctgcggcc gccggtttga caatgatgac cacaaacagc acagcggcaa
     6481 taatatgagg aacctgtatc aggcttttcg gatcaaacag catgccgacg gcaataaaga
     6541 agagcacggc aaaagcatcc cgcatcggca acgcgtcgtt ggctgccctg gcggcgaatt
     6601 cggagcggcc gacggccagg ccggcgagaa atgcacccag ttccatggaa accccgaata
     6661 ccttggcggc gcccacggcg atccccaccg ccagaactag caccgccaag gtgaaaagct
     6721 cgcgtgaccg ggttttcgca atgctttcca gcgccctggg aatcgcccat ttccccagaa
     6781 cgaccaccag gaccaagagc cccagaattt ttattcccgc cagccctagg acccagccga
     6841 tgttcaattc ggcatttggg ttttgcgaaa aaagaagggg caaaaggagc agcagcgcta
     6901 cggtgagaat atcttccaca accgtccagc cgatggcaac gtgaccgatt gtggcgtgca
     6961 gatcgtgcca tttggcgagc accagcgcca tgacgacggt gcttgccacg gaaatggcca
     7021 tccccagaat caatccggat atccagctcc agcccatcag atgcaatatc gaagcgaggg
     7081 cgagcgtgga gagggtgctc tgaatcagcg cgcccggaat ggcgagacgc cagacggcaa
     7141 agagctcctt taaattaaat cgcaacccta ttccaaagag gagcaaaatc actccgattt
     7201 cagcaaactg ttcggcgatc acccggttga ctactacgcc gggagtgaaa ggtccgacca
     7261 taacgccggc gatcaggtaa ccgatgatgg gagataattt aagacgagtg gcgatatagc
     7321 cgaaaacaag cgctccggcc agaccaaccg cgaaggtcat tagaatgctc aattcatcca
     7381 tattgtgtgc acctttttta aatccgaaga tctatcttgg gcagatgtgg cttgacttct
     7441 acgttgatga tccgctaatg tatgaaaaaa acatttaaag atcaactctt ttttgaaaag
     7501 ttcctgcctt gacgcttcat ttgaccggat cgaaaatctt ttaacggagg aacagaccat
     7561 gaaaagtagg ttcatcgtac tgatcgattt ctcgcctaat tccgaacaac tgctgcggtt
     7621 tgcccatgac tggagtaaga gggtgggcgc tgaattgctc gcgttccaca ataccatcgc
     7681 cctgtcaccc ttgatgacgc cttatgagac aaaaaccacc ctgatcgacc gggccaatta
     7741 cgaggccagg gaacggctcg taaaatttac ggaaaatgtt ctgccgccgg cgacttcgat
     7801 tcaacatctc gtatccgagg aaaatatggt ggccgtcttg cgtgaattac tcaaaaagcc
     7861 tttcagccat ttggtttttt tgggtatcaa ggggacggga cttctgaaga agatctttat
     7921 cggcagccag gccgtaaatg ttatcaacgg catcgacaac ctgatcgtcg ccatgccgca
     7981 acatgccccc tgttgcgctc ccgatgcgat ccacgtcgcc gtgcagaaaa cctatcccct
     8041 taatctcgtc gaattgaatg agattatccg cctcaatgga gagggggggt cgaaaatcat
     8101 atttttttcc gtcgtaacgt ccgaagacga ccgagaggcc acggagaggt atctcaaagc
     8161 gctgacgcaa ttatattccg acaagaagga gacggcttat gaactgtatc tgggagacaa
     8221 tgcctggcgg gacctcaaaa agatcatcaa acagaaacaa aaagaattca ttgtggtgca
     8281 aagaggatca agaatgttcc tggaccagat aaccagaaaa ttcctcatca acgatctggt
     8341 ttatgaaggc catacgccct tgatcatcat gccttgacaa actcagatga atccgcagga
     8401 cgcatgccgt ttttacccgc gggccaaacg cgaagaaagg caatttttat cagggccagt
     8461 caatcccttt tccccctcat gttttttatg aactttgcgc cggaatatca ggccaatttt
     8521 atgtttccgg aacccggaac gatgatgcgg gactcttcct ggcgttgcat ctctttggcc
     8581 tgggcgatca tcatagcgac aaagctctcc atggcctcgg ggaatttttc ggcggcttgt
     8641 gggaggtttt tcgcttcgat agggaagcgg atgggcaggg gaccctgctt agtgatcaac
     8701 tgggtattgc cgatgaacag tactttgcgt tgtttgtccg cctccccgtt ggccttgacc
     8761 ggtgtcatct gatgcaggga gcctacggtc atatcggtga agatttcctc ccgatagaga
     8821 ttgttctggt tgatgcgtac cttttccggt ttttgatctg ttgcggcatc cataatgcat
     8881 cctctcctca aaatttcatc agccggcatt accggctgtt gtttgtctct gtatccgcct
     8941 catagcacaa cctcccaaga aaacaaggac gtttttgata tgcccttgca gatgttcgat
     9001 aaatatgctg tggagtgact ggaaggaacg cttcgaccag gattaaaaaa ctacataaat
     9061 attaactatt tactttaata tattgaggca gcatattgta atgattaagg attcaataaa
     9121 aacgcttgca attttatgtt tctttcacat ataagcaaac ttctccggtg ccggggtcac
     9181 acaatccgcg gccggagttc tgatttacac tcttcgcgca cgtcgttgcg ttaacccgcc
     9241 ggtagagggc acaggaaggg gagatccgcg tctgcagcga gtgacgatcg atccggcccc
     9301 ccggtatacg gaaccggaaa aagcagtttg atccttacgt ttgccaataa aggagcaatt
     9361 atgtggaaaa aggaacaaat ccgccatgta atcaagcgga tggtcgctcc ggtcaccatc
     9421 atgttcatcc cccatcacca cgccaaacat tcctttaatc tgaacatccc atttttattt
     9481 atccccctga tctccatcct caccatactg ggcttcatct atgccggttc catggttgga
     9541 gacctggtcc gttatcaggt gatggagcgt caaatgcgcg actacaccgc gaaggtcggt
     9601 gaattcaatg ccgctttgag tgccgtcaag caaatggagc aggaactgaa aaccctgatg
     9661 tcccagggct ccaagggaaa gatcctggag aattacgaat ccgccgacat gggcacggtg
     9721 gatatgtatc agattcagaa acagattgaa atatcgatgg aaactgtggc tggtatcagg
     9781 gactacctta gaaaccagaa ggatctgtat atggcaactc cccggggatt tcccataagc
     9841 ggaaagatca cctccctgtt cggcagccgc gccaatccca tcaccggacg ggtggaattg
     9901 caccggggca tcgacatcgc cgcaccggcg gggacgacgg tccaggcgac tgccgacgga
     9961 gtggtgagct tttccgggtg gaaccggggc gggggcaata ccgtggtcgt cgaacacggt
    10021 cacggcttct ccacctgtta cgcccacaac cgcgccaatg tagtacgggt aggacagagg
    10081 atcaagcggg gggatgtcgt gggctatgtg ggggcgacgg gaaatgccac gggcaatcat
    10141 cttcattacg agatctggca ggatggccgg gtgatcaacc cccggcagag tctggaggtg
    10201 gcgcgtgttc catagaagag cggaccggct gaaaatggtc ctgggggaaa acgcccggat
    10261 taccggcgat gtggaatcaa ctggagcggt cttcatcgac ggcgccatcc ggggcaatgt
    10321 ggagggggcc aaggttatcc tcggtgagca cggttgcgtg gatggagaca tcaaggccgg
    10381 gacgatcatc atcggcggca cggttacggg acgtctagac ggcgcggaaa aggtggagat
    10441 caaggccacc ggccgggtcg ccggggacat cgagactaag gtcctggtta ttgccgccgg
    10501 cggggtattc aacgggaccg tccagatgga aggcgcgggg aattcctccg gggagtgcgg
    10561 tgacggcaag gtcgtggaat ttctcgtcaa ggaacagccc tgaccggcag tgaagatcta
    10621 cgccgtcgcc gacatccatg gccggaaatc aagactggaa cgaatacggt cggcggcagc
    10681 cgccgcgcgt cccgatctgg tggtcatcgc cggcgacatc accgggttcg gcaaccagga
    10741 cgtttttctt gccggcctcg acagccttaa ccttccgatc ctggccgttc ccggcaatac
    10801 ggacagcgcc gagctcgaaa aggacttgga gaaataccca aatatcctct ccattcagct
    10861 ccgtaaagag gtaatcgatg gggtgtcctt catcggactg ggcgggaccc tgagcgtacc
    10921 ctttctggaa aaactacgtc ctaaagacaa ccgcggtctc gatgccttag cggaggatct
    10981 gggggatgag gacattctgg tcactcatac cccgccctgg gggatcttgg accggggatt
    11041 cgcctccctg cacggcggct cgagatggct ccgggagctg ttggaaaggc ggcacccgcg
    11101 gcttctgatc tgtgggcata ttcacgagga ccggggcgcc gccacggtgg aaagaaccac
    11161 tgtggtcaat tgcagtatgg gtcgtaaggg tgccggcgcc ctaatcaaag ccaaaaaagg
    11221 cggcggcatg gagattgcct tcttatgatc ccggcattgc ctcaggcctg gacgtagcgg
    11281 ccgttcagtt cgaccaccag gcccttctcc atcaacctgt ccaggtgctt aacgttcaac
    11341 gcccgttccc cgaattcaaa aaaggccagt ggttcccggg gtttcttata tatcagccac
    11401 gcggcgacga tttcctgaag attgtgggga cgggcaagca aattcaagag gttggcttcg
    11461 cgctgatgga tgatgtcctc gtatcggcgc caccgctcgt cggcgttgtc cgtgaatacg
    11521 ccggtgtcat ggccggtgat ccacgtacgg gccggaattt cccggagccg ccggagggag
    11581 gagatggtcg cttcgatgct cgaattgcgg tcgccgtacc aggggccgaa ggaagtcata
    11641 tcgtaatccc ccacaaatag gacctcctgt tccgggaagt agaaggcgag atgccccggg
    11701 gtgtgtccgg gggcgccgat cacctgaacg gtcgtggtgg cgaaggaatg gacatcgcct
    11761 tcggtcaggt aggcatcggc ccggcggggg cgatagcgga aggtttccgt gagtacctgc
    11821 cgccagtggt cgcgatgggg ttcggacaga ttgtaccaat cgagaaggat ctccagatct
    11881 cccagcggcg gttcgtcccg gcggcagatc ctcaccgccg cacccgggaa aagatccaga
    11941 taggttatgt ggtcttcatg ccagtggctc agccacacct cgttcacccc ttctaggtcg
    12001 cggatctcca ggaggcgctt ttcgtcggag gccggatcga taattattcc cggtccctgg
    12061 atatacagcg aatgacagta gggatagcgc ccccggttga cgccggggat gaaccaaacc
    12121 ggtccgaaat gtatttcctc gcagcccata atggtgggga atataggcgg agctgcggag
    12181 tctgtcaagg ataatcggtc gagacctaac tatgctccgc cggtcagggc cgattgcatg
    12241 tgcttggcag cccttgatcg agatgaggcg ccggacacac ggtacgaacg gccagcacgg
    12301 ccgcacatgc cgggtatttg cttgaccttg ccggtaatat ctcctatagt ccgccccgcg
    12361 ctggggatca ttacgcagcg gaatatccat caagggaggt atgagaagtc agatgaagat
    12421 cattctttta ggggcccccg gggccggcaa gggcaccgtg gcaaaactgc tttcggaata
    12481 cgacggttcg gtacagatat ccacgggaga catcctccgc agcgccgtca agacgggctc
    12541 ggaactggga aagaaggcca aggcctacat ggatgccggc gaccttgttc ccgacgagct
    12601 gatcatggcg ctcatgaggg aacggttggc ggcgccggac tgtcgccagg ggttcatcct
    12661 cgacggtttt ccccggacca ttccccaggc ggatgccctg aagggcctgt tgcgggaatt
    12721 gcggatagaa ctggacttcg tcgtcagcct ggaggtcccc cgcgaggtca tcctggatcg
    12781 cttgactacc cgacgcacct gttcaaatcc cgattgccag gagatataca atatcaagag
    12841 caatcctccc cttccggacg gaaggtgtaa aaaatgcggc agcctcacca ttcagcggga
    12901 tgacgaaacc gaggcggcga ttcaataccg cttggagacg tacgagaaaa agacctcccc
    12961 cctgatcgaa ttctaccgca ccgaagggct tctaagacag tttccctcag tgagcagcgt
    13021 ggaaaccgtc gcggccgtca agcggcaact ggggaaatga tcgattacga cggagaactc
    13081 aatgccgaac agcggcgggt ggttctggaa cccggggggc cgctgctggt catcgccggt
    13141 gccggcagcg gcaagactcg gaccctgacc taccgcgttg cccgtctgat cgagtcggga
    13201 tgcccgccgg gacggatcgt cctggccact ttcacgaaca aggcggcgcg gtccatgatg
    13261 agccgagccg ccgatctgat gtcgacggaa atcagccgtc tgtggggtgg taccttccac
    13321 catctggccc accgggtcct ccgcgcccat gcccacaggc tcgattacga aaacaatttt
    13381 gtcatcatcg acgccgacga tgccaaacaa ctcgtcaacc gctgcctgac cgaaaagtta
    13441 aagaacctca cccccaaatt cccccgcagt gatgtgctcc gggacatcat cagcttcgcc
    13501 cgcaataccg accggcgcct cgaagaggtg attgcggacc gttttcctta tctcgaaagc
    13561 tgtctggaag gcatcatgac cgtggcgcgg cactacgccg atgagaagaa gcgcctcaac
    13621 gtcatggatt tcgacgacct gctcatcaac tggcgcctgc tgcttgcgga tcatgaggac
    13681 attctggccg actacagcga acgtttccgc cacatcctgg tggatgaata tcaggatacg
    13741 aacattatcc aggcggacat tacggacctt ttggcgtctc ggcaccggaa catcatggtc
    13801 gtgggcgatg acgctcagag catctatgcc tttcgagggg caaattatgc caacataatg
    13861 aaattccccg ataaatatct ggattgtaag atcttcaaac tggagatgaa ttaccgcagc
    13921 accccggaga tcctccacat ggccaacctc agtatccgga acaacgaacg gcagttcgaa
    13981 aagtcgctcc gggccgtccg cccccggggg atcatgcccg ttttggcatc gccgcgcaat
    14041 gtccagatgc aggccgaatt cgtcgcccag cggatcatcg aactgatcgg cgagggatat
    14101 tcccccggcg agatcgcgat cctctaccgg gcccatttcc actccctgga gctgcagatg
    14161 gagttgaccc gccggggcat tcccttcacc atccgttccg ggatccgttt tttcgagcag
    14221 gcccacataa aagatgtgac ggcatatatg cgcatcgtcg tgaaccccgg cgatgaaatg
    14281 gcctggcggc gggtcctctc cctctgccac cggattggcc ggcagacggc gggcaagatc
    14341 tggaaatggc tggcgaataa tagggagccc ctggcgatct ttttgagtga cgcggcactg
    14401 ggaaaggtcg gcgccgcggc ccggcccggt ctggcgggac ttcaggaact gctggcggcg
    14461 ctcaaggatg tggaaggagg cgatatgggg acgatcatcg agacgatctt gaggcgtggc
    14521 tatgagtcat acctccagtc gacatatccg gacgcggtcc agcgtctgga ggatctcggt
    14581 cagttggccg tttttgccac gcgattttcc acccccggag agtttttgca ggaaatggca
    14641 ctccttaccg gtatcggtga agaaggggaa gggcttgcgg acgcggacgc gggggaggga
    14701 agggtaatca tgagtaccat acaccaggcg aagggcctgg aatggtccgt ggtgttcatc
    14761 ccctggtgcg ccgagggcat gattcccctc ggtcgggccc ttgcggaccc ggagggggaa
    14821 gaggaggagc ggcggctttt ctatgtggcg gtcacccggg ccaaggacca gctctacctc
    14881 tgctaccccg ccgtggaata tcggcggggg atgggatatg tcgccgtgaa gagttcacgg
    14941 tttatcagtg agctgacgcc aaccgtctcc ccgtcccgcc gggggcggcc ttacgaacgc
    15001 tggctcgtgg aggaggatta ttgatagccg ctacagccaa cggtcggttt tctgcttctc
    15061 ctcgtactcc gcccgcaggg cgatctgggt ggcctcggcg atgtcctcca cccgtttgac
    15121 ccaatcctcg aacttaatat ccaccccaaa aataccgatc atctcatccc gatcatccat
    15181 aatcggtgcc gagacggtga tgcacagggc gccggtcatc ttggagatgt agaaattggt
    15241 gacatggatc ttccccgtct gcagaggccg gatgaaccac tcccggtcgg actggtccgt
    15301 accgacgccg tagttctcgt atttggcccg gtcctgaata ttggtgatgt tctttgtcgt
    15361 cttccggccg ttcatgtcca cgacataggc gaattggatg gaggggttct cctcgataca
    15421 ttgctgcatc accggttctt gttcctccgg tttcatcgtc ttgatggcag ggtgctcgat
    15481 gatctgcttc accaccgcca cggcggcctg ggccgctcgg tatttaatct tgtccagctc
    15541 cgacatgaaa agctcgggga tgtacttccg gacccgcttc tccatctctt cgttggagat
    15601 gcttgtgacc ctaccggact cgtattcttc atttatccac ttgtgaatct tcgagattcc
    15661 tgggtgacgc ttgtcgatct ccgctgcgtc atgaaggccg aaatgggaat tgatccagaa
    15721 ggcaatcccc gcctttcctg acttgtcggt gatcatgggc acgatggggc gtttaagaag
    15781 cttggtggtg tcgaagatat tgtatatttc ctcacatttg atcagcccgt ccacatggac
    15841 tcccgcccgg gtgacgttga aatcggcgcc cacaaaggga tagttggcgg ggatcttgtg
    15901 gccgatttcc ttttcgaaat actgggcgat atcggtgatc accgtggtgt cgatgccatg
    15961 gtcatttccc gtcagggcaa tgtactcaat gatcaatgcc tcgatgggtg ggttgccggt
    16021 ccgctccccc aaaccaagaa gggtggcgtt ggcggcacta cagccataaa gccaggcggt
    16081 ggtggcgttg atcaaggcct tgtggaaatc gttatggccg tgccattcta ggaggtgtcc
    16141 gggaacgccg gcatcgtcga tgaatgcccg gaccagctta tccacacttc tgggaagtga
    16201 tgccccggga taagtcacgc cgtaacccat ggtgtcgcag agccggatct tgatatcgat
    16261 cttgctttcc tcgcggagct tcatcaactc gatggcaaag gggatgcaga agccgtagat
    16321 gtcggcccgg gtaatatcct cgaaatggca gcgggggatg atgccctccg ccagtatgga
    16381 acggacgata ttgagatagt cgtccaaggc ctgtcgcctt gttttgttga gcttgaggaa
    16441 gatatgataa tcggaaacgg acgtaaggat tcccgtttcc ttgagtccgg cctcctttac
    16501 caggcgtacg tcctctttgg cggcccggat ccagcccgtt acctccggat agcgcagtcc
    16561 cagggaaagg cacttatcca ccgcctcccg gtccttcttg ctgtacagga aaaattccgt
    16621 ctggcggatc accccgtttt caccacccag acggctcatg aggatgaaaa gatcgaagat
    16681 ctgctttacc gtgtaagggg ggcgggcctg ttgaccgtcc cggaaggtgg tgtcggtgat
    16741 gaagatttca tcgggcggcg tgatgtccat aagtttatga tcaaaatcga tcctgctgac
    16801 ctgttcgtag gggaaaacat ctttttgcag attcggttcg gcgatatcct tcagttcata
    16861 gcgccagaat ttagtatgct cgtggtccag gatcttcttg ttcttattcc atttagccat
    16921 gtcgggaatg ctcctgaaaa caagagaggg cgcctgagat ttcacgcgcc ctctcagata
    16981 aactatgata atgctttttt aattcaccgg gggtctgctg tctttcgacg gcttgtatgg
    17041 ttttttagcc ccggcctgct tacgtgcctt ctctacatct ttcatggtaa gcccagagcg
    17101 ggggatcttc agcttctggc ccggataaat gaggtttgct tttttgatct ggtctttgtt
    17161 ggcctggtaa ataatcggcc agaggtaagg atcgtcgtaa atatccttgt atttggagat
    17221 ccaccagagg ctctcgccct tcttcaccac atattccgtg accagaccgg cttcctttgc
    17281 ctcaggtttg gcttcagtag tcgctggcgc cgcggccact gtcttcgttt cggaaaccac
    17341 ctgctgatcg ccgggagccg cggtgggtcc ggcggttacc gcctttttcg cgcaaccggc
    17401 aaaggaaagg accatcatcg ccgccgccac aataccgcat acaaacatcc agtgcctttt
    17461 gtattgcatt ttaaaacctc cttattgaaa aatatgattt ttgactactc gataacctct
    17521 ataaacatga ccagcgtttg tcaataaaaa aaatccctat tgaacgattt tcaaagacag
    17581 ttccatgagc tgctcgatgc tcacctggga aggggcgtcc gtgagtaggc aggatgcctt
    17641 ctgggtctta gggaaggcga tgacatcccg gatggaatcg gccccggtca tgagcatgat
    17701 caggcggtcg aaaccaaagg cgattccacc gtgaggcggc gttccaaatt cgagggcatc
    17761 cagaagaaac ccgaatttat tgcgtatttc ctcctgttca aggcccaggg cggcaaagac
    17821 ggcggcctgg acatcctttt tgtggatgcg gatgctgccg ccgccgattt cggaaccgtt
    17881 caggacgaga tcataggccc gggctcggac ccggcccgga tcgctctgca ggtacacgtg
    17941 atcttccaaa atcggagcgg tgaagggatg atgggtggag gtgaacctct tttccgtgtc
    18001 gctgtactcc atgagaggga attcggttat ccaggtaaag gcaaaggcct tggggtcgat
    18061 gagattgagc ttcctagcca gatggaggcg gagattgcct agggcgtcat tgaccacccc
    18121 cggggtgtcg gcgataacca ggagtaggtc gtcgcttttg gcctccagcc gagtttcgat
    18181 atcccggatc tcaacaggtt tgaggaattt ggcgataggg gaagtccatc tgtcggcgtt
    18241 gatgcgggcc caggcaaggc ccccggcccc ataatcggct acgtaatcct gcaactggtc
    18301 gatgtccttt cgggagagct ggcgtcgatc ctcgaccctg atggccttga tggcgccccc
    18361 ggaggcggct acctcctgaa agaggttgaa atcggcatga cggaggatat ccgtcaggtc
    18421 catgatttcc attccgaagc gggtatcggg gttgtcccgg ccgtatctct ccatggcctg
    18481 gcgataggac aaccggggaa gcggcagggc gatttcgatt cctaggcagg aacggaagag
    18541 atgggcgaca aagccttcca taatcttcat caggtcgttt tcgtcgatga aggacatctc
    18601 gatgtcgatc tgggtgaatt ccggctgtcg gtccgcacgc agatcttcgt ctcggaagca
    18661 cttgacgatc tgatagtagc ggtcgaatcc ggataccatg agtaactgct tgaagatctg
    18721 cggagattgg ggcagggcga aaaaggttcc cggattcacc cgactcggga ccaggtagtc
    18781 ccgggccccc tctggggtgc ttttcgtaag caccggggtt tccacttcga tgaatccctc
    18841 ccgttggaaa tactcccggg tggaggcggc ggctttgctt ctcaggatca gtttctgttg
    18901 gatgtctttc cggcgcaggt ctagataacg atatttcagg cgtacgtttt ccgatatctc
    18961 ggcggcgggg gcgtcgatga gaaagggcgg ggtgcgtgac tcgttcagaa tctccagctc
    19021 gtcgatcatc acttcgatct ttcctgtctt caactccgga ttttccatct ccgggggccg
    19081 caggcagacc tttccccgca cggcaaggac gtattcactt ctgattcggt gggcttcctg
    19141 gtgggagtcg gcttcgtatt cgggattgaa aactatttgc acgaggccct cccggtctcg
    19201 taaatcgacg aatatcaccc cgccgtggtc tcggcgccga tgagtccagc ccattagcac
    19261 cacttcctgg cctgcttcgg aaaccgtcag tgccccacaa tagtgggtgc gtttgtattt
    19321 ggtaatgaaa gctgacaatg taaacctctt ctctccaagt tttcgttttc aaacgagcat
    19381 gtctatccag gcgaacgaat cgccgaggct gacccgtacc tgctctcccg ttaccatatc
    19441 tctcatctcc gcctgctgcg cctcgatttc acggtcgccg atgatcagaa cgcgccggca
    19501 tttcagtttg tccgcccttt tcatctggct tttcaaaccc cgggactggt agtccatttc
    19561 caccttccgc ccccggaggc gcaaattatt gcacaggcga taggcaaatt gctctgccga
    19621 aacgcccagg gcggcgataa aaaggtcagg cagccgggaa gggggggggg cggacgtatc
    19681 ggcggtcagg gcgaggagcc gctcgaagcc gatggcaaaa ccgatccccg ggatgtccgg
    19741 tccgccgagg tcgcggatga ggccgtcata tcggccgcca ccggccacgg cattctgggc
    19801 gcctagaaat tccgtggtga tttcgaaggc ggttcgggtg tagtaatcca gaccccgcac
    19861 catccgggga ttgacggtat aagcgatgtc gaagatggcg agggaagaaa gtacctgcgc
    19921 gaaatgatcc ttgcattcct cgcagagaaa atccgtgatg cttggcgcct tggtgatgat
    19981 ctcccggcag gcctcaattt tgcagtcgaa gacccgcagg ggattggtgc cgagacggcg
    20041 gcggcaatca tcgcagagat ccgcctcttt tcccgccaga aaactttgga cggaggtgcg
    20101 ataggcggga cggcaggccg ggcagccgag agagttcacc tcgagatgcg gtgattccat
    20161 accgacagat tggagaaaat gtaggagcat aaggatgact tccgcgtcga tgcgggggtc
    20221 ctcatggccg agaatttcga catcgatctg atggaactgg cggtatcgtc ctttctgggg
    20281 acgttcgcgt cggaacatgg gaccgatggt gtagagcttg gcgatcggat cggtggcgtg
    20341 gaggtgatgt tcgaggtagg ccctgataat cgaagccgta gcctccggcc tgagcgtcag
    20401 gcattcatcg ccccgatccc gaaaggtgta catctccttt tccacgatat ccgtggcttc
    20461 cccaatcccc cgggtgaaga gatctgtctt ttccaaaacg gggacgcgga tctcgcggca
    20521 accgaaggcg gtgaaaattc tccttgcttg atcttccaca tattgccatc tgcccgtttc
    20581 ctcggggagg atatccttaa aaccttttac agcggtaatg agttccatag actttcgatt
    20641 caggtgacga atttcataaa aaggtactaa ctatcacatt accggggtaa tggcaatggg
    20701 aatgatgtca ggaaaaggtg gctgggtaaa taagacgaaa aggtgccggc aatcttcctt
    20761 tccgtcccga atgcggcggt gggtaaggtt ccgttttcaa cttccgtttg ctttccggag
    20821 aaagctgccg gggtagcggc gatccaggcc ttctgttcgc aaaaaacgcc tgccttcttc
    20881 aaggctggca aattctccga ccaagacccg attccagacg cctttacctt ttattgacac
    20941 ctcttctata cttacatgcc gatatttatt cttcaaccgg ccgatctcgg cggcggcgct
    21001 accggtgtca ggaaaggcgg caatctgaat ccggtaggtt ttctgcccgg gggatgactt
    21061 ttctgcgggt atttcttttt tatcttttat tggtgaggtg gttgacgaca caggagcggt
    21121 aatcttcatt tccattaatt gggagggagg agagggcgac tcttcagtag cgggagccgg
    21181 aataggcgga ttcgccgtta cttcaggcaa agggggacgg cgccaccctg taccgtcatg
    21241 ataagcgaca tcaccggtaa gtctatggat gcgcaggggg aagatacgtt gggaaaccgt
    21301 cagcgtatcg tagtgataaa gctcaggcca aaaaaatgca acggctgcaa ttaacagcgt
    21361 cggaacaccg atgaaaaaaa accagattcc ccatcgtccg gaatgcttgt cggtcgagac
    21421 aacgtcctcg gcgtggggag tggccatgat ttccttttga tccgaaacct ccggcttatc
    21481 gataagattc ggggctgggg cggcttcggc aattggcggc atctctttat gacgcagagc
    21541 atgaaacttg atgagatttt cataggcctg ttcaacttcc cggcgtttat atgccgcctt
    21601 ttcatcccag aaagggactt gcgggtaacg gccctcccgc caggcttcgt tcaatttctc
    21661 atatgccgtt tggatctcct cccgcgaggc gccgggagca atctggagaa tgttgtaggc
    21721 ggcatggaaa tcgattacgt tatcaggggg aagatccgga tcatcgagag cggcaacctt
    21781 atgatagagg gtttccaact ccctgatgag attgtcgttg ccttcgggaa catccgtttc
    21841 tcttcctggc ctttcttcct cagtcaaggc gaacctccct gttcggtttg cagttttttt
    21901 atataacctt tgattatccc ctcgggatca caatgaatga tctgggcgta tattctcaag
    21961 aaacctttca agtatgcaag aggcggcagt aaatggtatt ggtcgccctc cagggcctcc
    22021 agcatggaca tcttgatctt actttgaata gcgatttctt ccagagcaac ccctgcctgt
    22081 aaccgccagt tttggagatc caggccggag atggcttctt tttgctggag atcacatatg
    22141 ctgaccggat tcggggtgga tcttgtttcc ggggcgacca aatctgtctc gtcggccggt
    22201 acggaatcgg tttcgatact attcatatat gttgtatctt cgccgacatc atcgggatga
    22261 ggcagtcgaa tcagtttgcg gcctcgagcc gaagtatctc gtgtgaatgg atacaggggc
    22321 gcaggttccc ggacccgatc ctgataacac atcttttcat tcaaaagtcc ctgagcgata
    22381 agcctctgat cataattccg gcgcgatctc tcatcaatca ggaccagata tgctttttcc
    22441 agttccgtca aaagttcctg cctttcttct tcacggaaaa aggaatagga ggcaagagac
    22501 tcatgggcgt agagctcatg gatctcctgg taggcccggc ggatctcgta aggagaggca
    22561 tcagggttga cttcgaggat ttcataaaga gtatggccgc gaaagctgtt catttcgtta
    22621 actcagtgct tagaaatcac tcatactacc ggacaggtcg catttgcctc ccacgacgga
    22681 agacgaaagc aggttgcgta ggcattgcct taggtcatgg accgtctgcg taaagggata
    22741 aaggtcgatg aaaaggctgg ttttgcagac ggcgttgtga actctatcgt cgaaagcgac
    22801 attgccgaga aaaactattt tcagatttaa atggcgggaa atgatcttgc agatcattgt
    22861 gccgatatga ggattatcct gtttacgatg ttgattgaca atgagtttga actgaaagga
    22921 ttccagctca ccggccaaca gttctccccg ttccggatcc aattccctga tgacggcgag
    22981 taggaggtct ggattagtga ccttggccat gggatttctc tcctctgctt ctcgggccag
    23041 tttctgaaaa tgatgggtgt ccaaagcttt tcgaatcttc cggaaataaa cggatctgat
    23101 gaggcgatag atattctcta tggccgtggg ttccggggtg gttacaaaga tcccatgatc
    23161 tgatatcatg aagaaatcca gggtgttgaa ggacgtaccg gcacctagat caaggagaat
    23221 gtagtcgtag ggcagcttga cgatatgacg gagaaccttc atcttctgct catgagcgag
    23281 gttggcgatg tcgaggttgt tcatggcgcc gctgatcata aacagcaatg gcgtgggagt
    23341 ggcgatgatg gtgtctgcca ggttagaaac ccgtttgttg ataaagtcgg agaggctcaa
    23401 acgggggtgg gtaacgccga tgatggtatg caggttggca gctcccagat cgacgtcgat
    23461 caccagggtc cgaaagccct cccgggccag cagcaccgcc agcgatccag agagaaagct
    23521 cttgccggaa cctcccttgc ctccaccgat gggtagaatg atcgccatta ataatcctac
    23581 cttgaatgac gcctctaagg atcttgatgg ggcaaaatcc cggagcgttg cttcttcatg
    23641 cccgctggcc agcggcgggc cgataagatg cgtataagaa tcatgtgata ttgattacgc
    23701 atcatgaatt attgattaat taacctttga tgtcaagtat ttattgggac tgccaacgaa
    23761 gatttcaatt ttttggggcg agggggatcc accctccgat gtcaaggagg ggaaaataaa
    23821 tagtgcctga ccgtctgttg cagcccgaat cattccctgt aagtcggtac gataaatctt
    23881 cgcaccggcc tttcggagcc gcgtgaggac ttcgggatgg gggaggagaa aaggattatc
    23941 ataccctgcc gagatgattg ccgccgccgg tttaaccgct tcgagaaagc ctgcggtggt
    24001 agaagttctg ccgccatgat ggggcacaaa gagcagggag cttaaaatgc gcccccgacg
    24061 ggagacgatg gcttcctcgg tggctccgga aatatcaccc gtgaaaagga aggagacgcc
    24121 gccgaaggtc atcctcattg ccagcgaccg atcattggtc tccacataac tgctctcggt
    24181 ttcttcatcg gtccctgagc attcgggatt gagaaattca atgcggacat catttataat
    24241 gataggatcg tcttgtgatg accggcggtg atgggggatg ccccggtcct ggattatctt
    24301 taatagttct ccatattctt tgctgtttct cttttcgccg gtactccata cctcgcgcac
    24361 ggaaaaatta tttagaatga aatgcagccc ctggaggtga tcggggtggg gatgggtcaa
    24421 aacgacgatg tcaatggcgt cgatccgttc ccgccacaag tagggggcga cgatgtattt
    24481 cccaacatcg aagatgctgt tcggaaaacc gccgccgtcg atgagcatga tcttgtcggc
    24541 ggggaaacga accagggtcg aacttccctg gccgacatcg atagcagcgg cggagaacac
    24601 accatgattc cttcctgttt catgaagtag gattccgttt accacaaaga acagtacggt
    24661 ggcggccagg gtgtaatgga cccacaaggg tggtttggcg gcggttgcag ccttggcggg
    24721 atcgggcgac gctgactgag gcagcagccg cacaaccgcg taaatcaaca gataaaatac
    24781 ggcgatttcc aggatgctcg gcgtggtcaa atagcgggaa gaccagggaa gggaggcgaa
    24841 gaagtcgttg acagttaggg agttcatcac cagcacccca gccaggtcta ggcaaatttt
    24901 ggctaatgtt gaagaccagg gaagaaagaa caccgcggcc agggaggcgg gaacggcgag
    24961 gatgcccagg atgggcacaa ggatgatatt ggcaggaaag gcaacgaggg aaatgcgatt
    25021 gaaatataag gcaatcaagg gcaacgtccc cagcatggcg ctaagggata cgatcataaa
    25081 cagggcggtc tggtagcgga gtcttcctat gagatgggcg aggagggatg atgttgagcg
    25141 attcgatacc atcttgggta caaaggcggt caggcggggg gtgatgaaga ggatcgccgc
    25201 caccgccgca aaagaaagtt gaaaggagat atcgaacagg gccgggggat aggggactag
    25261 gatgaacagg gccgccatgg ccagggtatt gtagagatca cggtttctgg cgatgagcag
    25321 ggcgatcatg aaggccagga tcataagggt ggcgcgccat accgacatcc cggcgccagc
    25381 aatgaatgtg tagaggatca ctggcgggat ggcggctaaa accgacagct tgtgcatctc
    25441 gaagcgcagg aggagatagg gaaagcgccg tagcagtgaa cggacgagaa agatggaaaa
    25501 gaaggcgacc agtcccatat tgaatcccga aatggcgata atgtgggagg tgccggtctt
    25561 gctgaaggcg tcccggattt cccgaggtat ctcctgctga tcgccgagga tgcaggcctt
    25621 gacgatctcc cgttccggaa aaggggcgtg taaatccacg aagctgcgta tctgggcgcg
    25681 gaccccttcc agtcgctgcc ggagtggatt gcccaaaccc tttcgcagta aaatgatgcg
    25741 ggaatcatcg ggaatgaagc ctcgcaccag aatcccctga aatttgagat ggcgttcata
    25801 atcgaagccg ccggggttat ggaagttccg cacccggcgc aaccgggtcc gggcgctgac
    25861 agcgtcacca taggcgaagg tccgccggcc ttggtaacta ataaggatct ttccgtggca
    25921 ggggtgttcg gcgccagtgc cttcagtcat cttcagggct ttaacggtga gatcggtctt
    25981 ctccggaaaa tgttgcggcg cctcgctgac cagccctgtg atcgtcaccg gctcagattt
    26041 cagcagattg acaatatggt ctccggccgg ttctcggtaa agatatagat tcatctggag
    26101 ccaccctaaa acgaaaaaag ccccggcaag gaaaaatgag gcggctttgg atcttcttcg
    26161 gatgatgcaa accaaaagaa acagacaagc ggcaaggagg gcggtcagga ggggcgccgt
    26221 cgccacttca taaacggatg aacaggcgat gccggccatg agggccaggg tgggagccgc
    26281 aaggggacgc cccatatcat gtctccggaa gaagtttccc ttgcatatgc aggaaaattc
    26341 actatgatgc aagccgcctt tataatcact tatgaagggg ggaacatgac aacaaatgac
    26401 gaaagacgct ggctgagata ttacggcgcc gtgcctccct ccctggacta cccgcgggcg
    26461 acgatgtacg aggccttgat gagatcggcg gagcgcaatc ccgaggcgat cgcctatgat
    26521 ttcatgggcc gcacctccac ttaccgcgac tttaccgccg aaatcgatcg atgtgcccgg
    26581 gcactgtatg cgctgggatt gaagaaaggg gaccggatta cgatttccat gcccaccacc
    26641 cctcagggta tcatctgctt ttatgccgcc aacaagatcg gcgccgtggc cagtatgatt
    26701 catccccttt ccaccgccca ggagattgcc ttttatctcc ggatcagcag gagccgtttc
    26761 gctcttacca tcgacgcctt ttacggcaag ttccgggagg tacgggacga tacaccgctg
    26821 gaggtcctca tcctcacaaa aatttccgat tatctcgatt tgttcaaaaa gatcggtttt
    26881 tacctggcca agggccggaa gatcccccca gtgccggcag acccgcaagt acgatggtgg
    26941 cgggagatca tggcggcatc ctatccgcct gtccctgaag tggccatgga cacggaagat
    27001 ctggcggtaa tcctctacag cggcgggact acggggaccc ctaaggggat catgctgtcc
    27061 aatatgaatt tcatcagcga ggggatgcag gtgtcgactt ggggtaatct gtcgaaacag
    27121 gataccattt tggccattct tcccattttt cacggcttcg gattgggggt ctgtgtaaac
    27181 gccgcgttca tgggtggcgg caagagcatt ttggtgccgc agttcacgcc ggaaatcgtc
    27241 gccaagctga taaggacgaa acgccctaca tttgtagtgg gtgtccccac actcttcgag
    27301 gccttatgtc gtaatccggt gtttcaaaac gctgatttga gctgtctcaa ggcgaccttc
    27361 agcggtgccg acaccctgcc cagaccggtc aaggagaact tcgaggccct ggtaaaaagg
    27421 cagggtggaa aggtgaaact cctggaaggc tacggcctca cggaagcggt cacggccatt
    27481 atggccaccc ctttgcagga ataccgggaa ggcagcatcg gggttccttt tcccgatatg
    27541 ctggcgaaga tcgtcgccat cgacaccacg gaggaagcgc cagtgggggc cgaaggtgag
    27601 ctctgcctcc atggaccggc ggtaatgatg ggctatctcg accagccgga agagacggcc
    27661 aggaccctga ggaagcatcc cgacgggcgg gtgtggctcc ataccggtga tatcgcctcc
    27721 atggaccagg atggtttctt ctatttcaaa ctgcgcctca aacggatgat caaatcttcc
    27781 ggcatgaatg tctatccggc ccaggtggag gatatcctct accgccatcc cgaggttaag
    27841 gacgcctgtg tcatcggcgt acccgacgag gggcaggtgc agcgggtcaa ggccttcgtg
    27901 gttctgaagg acatgaacaa agccggcgaa gcaatggaag aggaactaat ccgccattgc
    27961 cgggaacagc tcatcaagtg gagctgcccc cgggaaatcg aattccggga aatcctcccc
    28021 tgcaccttgg tggggaagat cgcttacaac gctctggaac aggaagaaat cgccaaactc
    28081 aaggcggcgg ggaaatacgc cggaaagtag gcttatgtca aaccggtcag aaccgggaat
    28141 gaattatgat gcccatataa agaacaattc taaacaatca agccctgtaa taaaagtgtg
    28201 cataaacgga aagggacttg ttaatattaa tcacttcata aaaaattggg aaaggacatt
    28261 tgtgctgccg gcaattatca attatcaatg atcgttgttt gccggttcag gagcggaaga
    28321 tggaagaggc aataaagtac atggcggaaa ttacggcggc tcccttaatg tacggccgcc
    28381 gcattcagaa ggaaaccggc agaagggtcg tcggctatgt gtgcagctat accccggagg
    28441 agattatcat cgccgccggc gcccaccccc tgcgcctgtt cggcccgatg ggggagttgg
    28501 aatgcgtgga tacccaccta caatcctact gttgttcggt ggtgcggggc attctcgaga
    28561 ccggtctcaa aggcgatgcg gatttcctcg ccggaatggt ctttcctcat acctgcgatt
    28621 ccatccagcg cctttccgat ctctggcgga taaatgtcac ccatggtttt cacctcgatc
    28681 tcgtcctgcc ggtgaagtta aataccgaca gcgccaggaa atattatctc gacgtgctcc
    28741 gacggttccg gatggagttg ggaaagaagc tcggggtgga tatcggcgac gaagccctga
    28801 ggaaggccta tgaaacgctt cgggagattc gccgtctcct ggccgggatt tatgaactgc
    28861 gcagcgccaa tccgaccctg ctgtccggcg atgatctcct gaccatagtc aaggccgcca
    28921 tgatcatgga ccgccgtgag gcaagtgatc ttttacagcg tgtcgccacc gggctgaaag
    28981 cgaaaattga agcacataaa tcgctgccgg aatccgtaaa ttcttctccg ccccaggaaa
    29041 aggaaatcaa gcggctcgtc ttggcggggg gcatatgcaa tcacccgcgc ctccacggca
    29101 tgatcgaggc ggctggcggc gccgtggtat gggacgatat gtgcacgggg gcgcgttatt
    29161 ttcaaaccgc cggtcccggc gtcgaaagtg atgggtcaac agcagccggt aagggcgata
    29221 ccatggcggc catcgcagag cgttatctca atcgggaggt ttgtcccgcc aaacacaatg
    29281 gcttgaccag ccgtgcggag gcgatcattt ccttggtcag agaaaagcag gcccgcggcg
    29341 tgatcttgct gcatctgaaa ttctgcgatc ctcatgcctt cgattacccc tacctgaaga
    29401 acgccctgga tcgggaaaat ataccccatc tcatgctgga gatcgaggac cctctcccgg
    29461 cggaaggaca attgcgcacc agattcgaag cctttttgga aatgttctaa acaggagtga
    29521 ttaatggcca caaacatcga caaggaaaaa cgaaaaatta aatccgtcaa gaaaatgaag
    29581 gaaataatga cattatatta tatcgatgcc aagacggccg ctgataacgg caaaaaggtc
    29641 gcctggatta ccagcggcgg tccggtggaa cctctgattg ccatggatat catcccggtg
    29701 tatccggaaa accacggggc gatgatcgga gccgccaaaa tgggcgtcga tctctgcgaa
    29761 aaggcggaag ccatgggtta cgccggtgat ctttgttcct acgcccgctc cgatatctct
    29821 tgcgccacgg tgaacggcgg tcccattggg gggctcccca ggccggacct gctggtctgc
    29881 ggcaacaaca tctgcggcac cgtgctgaaa tggtatgaaa tccaggcccg ctatttcgat
    29941 gttccgctgt ttatcttcga cacgccgttc tgccacaccg ctttccaccc cgagatgcgc
    30001 cgttatgttc gcacgcagat cgatgaatat atagtctttc tggaatcggc aacgggaaag
    30061 aagctcgatc gggaccggat ggtcgaggtt gggcgcctct ccgtggaggg acagcgcctg
    30121 tggcaggccg tacttgatac gacgatcaac aagcccgcac ccatgagcgc tttcgacgcc
    30181 ttcttccacc tggccctcat cgttactctt cgtgggaccc ggcaggccgt ggattattac
    30241 caggaattgc tggcggagat gaaccagcgg gtccgtgacg ggatcggcgc cgttccccgt
    30301 gagagttacc gactgctgtg ggacaacctt cccatctggt accggaccaa gtggctgtca
    30361 gacaagttcg ccgcccatga cgcctgcctg gtggccgaca cctacacctc cgcctggtgc
    30421 ggctcactcc gctatatgga tgaaaatgat ttcctaacct ccatggccga ggggtatacc
    30481 cggatctatc tgaatatcgg cgtcgacgag atggcgcgaa tggtaggtga aatggtggac
    30541 aagtacgatg tggatggcct tgtaatgcac tccaaccgga gctgcaagcc atactccttc
    30601 gggcagtacg acatcaggaa gatcgtggaa gacggcaaag gcatcccttc tttgattatt
    30661 gaagcggata tgtgcgatga acgcaatttt tccgaaagcc agatcgagat gcgcattgag
    30721 gccttcattg agatgctcaa gcagaaagac gggcaataaa ccaatctgag tgttggagaa
    30781 aggtgacgga cgactactga aaaaagaaaa gcttcgaaat atcgccatca tttcccatgt
    30841 tgaccatggc aagacaaccc tcgtagatgc catgctgagg cgaaccggcg ccttcaggga
    30901 gcatcaggca atagaggacc gggtgatgga ttccctcgat ctcgagcggg agcgggggat
    30961 aacaatcatg gccaagaata ccgccatccg atatggcaag gtaaagatca atatcgtcga
    31021 taccccggga cacgctgact tcggcggcga ggtggaacgg agcctcaata tggtggacgg
    31081 agccattctc ctcgtggatg ccagcgaagg gcccctgccg cagacccgtt ttgtcctcaa
    31141 aaaggccctg gaacggcgca tgcccataat cctcgttatc aacaagatcg accggagcga
    31201 tgccagaatc gaagaggtca tcaacgaggt atacgatctg ttcatcgatc ttgacgccac
    31261 ggaagagcaa atagagtttc ccattctttt cacccaggcc aagcggggcg ttgctcaccg
    31321 aaaaatgggc gacgaggccg cggatttagg ggcgttgttt gagacgatcc tgactgccat
    31381 tcccggtccc gatggtgatg acgaggcacc gccccagttt ttgatcacca atctcgacta
    31441 tgatccctat gtcggccaga tcgccatagg ccgccttatg aacggtaccc tagagatgaa
    31501 ccgttcctac agccttagtg gagaaaacgt ggttgtgtcg ggcgtcggat tttccgccct
    31561 ctacacgttt tccggtctga agaagatcgc cgtggagaag gtagaatccg gggatatcgt
    31621 cgccttcgca ggggtggaaa aaatcagtat cggcgacacc gtctcggcgc tggacaatcc
    31681 tcgccccctg ccgagaataa aagtcgacga accgacggta tccatgatct tttatgtcaa
    31741 taatggtccg ttggccggac gggaaggaaa atatctcacc tcccggcacc taagggagcg
    31801 gctggaacgg gaaagtctcc ggaacgtagc cctccgggtg ataccgacgg aacgtgcgga
    31861 cgcctttgag gtttgcggac gcggggaact gcagatggcg atcctcatcg agaccatgcg
    31921 ccgggagggt tacgaattca tggtcagtaa accccgggtg atcaccaagg aagaaggtgg
    31981 ccgggttctg gagccggtgg aaagggtctt tctcgatatt ccggaggaat tcgtcggcgt
    32041 ggtcacggag aagctgtcca tccgaaaggg ccgcatggcc aatctggtca acaagggtcg
    32101 cggtcgggtg aacatgattt ttctgattcc cgcccgggga ctcatcggtt ttcgcagcca
    32161 ctttctcacc gatacaaaag gggcgggggt gatgaacagc cttttcgaag gttacgagcc
    32221 ctggtgcggc gccattcccc agcgtctgac cggtgctctg gtcgccgacc gagcgggacg
    32281 ggcaacggcc tatgcctgcc atgccatggc cgaccgcggc gaactgatcc tcgccccggg
    32341 cgctgaagtc tatggcggga tgatcgtcgg agagaggaac cgctcccagg atctgaacgt
    32401 aaacatcgtc aaggagaaga agcttaccaa catgcggagc gccacggcgg atgccacggt
    32461 catcctccgt cctccccggc tcctgtctct ggatcagtgc atcgaattca tcgccgccga
    32521 cgaactcatc gaagcgactc cggaaagcat ccgtctccgc aagcaggaat tggacgccca
    32581 ggtccggatg tcccgttacc gtgaagagaa atggtcgtga agacttgccg gtagacttct
    32641 acacgtaatt tcggtgctgg atcgcttcga tataacccgc ggcgccgggg gagatctgag
    32701 cggcattggc ttcgtcggta tccagatgca tgtccaggcg ataactggga ttcacacgga
    32761 ttaggacgtc gccgaatatc agctcccgca cccccttgac cttcaccatg accacgtcct
    32821 tgtcccggag tcccaggctc aaagcctctt ccggagagat gtggatgtga cgtttggcgc
    32881 agatgacccc cttgtccagg gtcaccgttc ccacttctcc ctccatgatg acgccgggcg
    32941 tcccttcgat atcaccggaa tcccggatgg gggcatcgat gcccaggcga aactgttcgg
    33001 tgcgggcgat ttccacctgt gactcttttc tcagggggcc caggattcgc accttttcga
    33061 tccggccttt gggcccaacg agatttaccg tttccacggc ggcgaactgg ccgggctgac
    33121 tcagttccga acgcggcgtc agggaacgtc cggggccgaa gagggccgtg aaatcatcca
    33181 aagacaaatg gacgtgatgc gccgaaatgc tgaggggaat cgctttggac tgaagccttt
    33241 ccaactcgct cacagcgagg gatctcccca gggcatgcac ggtttcccgg gcgatcatcc
    33301 gctcctcgtc ggcaggcacg accaaaacgc ggatcttggc ggaaggatcg gagatatcga
    33361 cgatctgccc ccgcatggcc acggttttac gattgatgtc atccgtgacg gcaatcccga
    33421 agacctccat tccctggcag atgcgggcac ggatttccgg ggagttttcc ccgataccgc
    33481 cggtaaagat gaggacgtcc aagcctccca gggcggcgac ataggcgccg atgtattttt
    33541 tgatccgata gcagaagaca ctgagggcca ttttgcagcg catatctccg ccttccgccg
    33601 ctgccagtac ttcccgcata tcgttgctct tgccgctgag gcccttgaga ccgctttcct
    33661 tgttcagtat ccggtcgaga tcgtcgtaag tcataccggc atggcgcatt agatggagca
    33721 gcgctccggg gtccacgtcc ccgccccggg tgcccattac cattccctcc agaggcgtca
    33781 tgcccatgct cgtatcgatg ctgcgcccgt gatccacggc gcatacggag gctccactgc
    33841 ccaaatgaca ggagataatc ttgagatcgc cgatgggaat tttcaatgac gtggccgcgc
    33901 tgagagatac gaaacggtga ttagtgccat gaaaaccgaa ccgccggatc tgctcttcgt
    33961 cacatagctc catgggcagg gcgtaacggt aggcggcctc cggcatgttg agatgaaagg
    34021 acgtatcgaa caccgcgacc tggggcaccc cggggagaag tgttttcagc gtctcgatcc
    34081 cctcgagatt gtaaggatta tggagagggg cgatgggtat gcattctttg atggcggtca
    34141 ggacatcatc attgatggtc atggaactgg agaagcgctg gcctccgtgc acaacccgat
    34201 gacccacggc cgagatgcca tgcacatcgc ctacggcgcc ttgcttttcg tcggataggg
    34261 ctcggaccat ggcatgaaag gcatcgctga ccgtagcgat gccatcggcg ggcagggttg
    34321 tcttctgccc ggccttcttg atgatgtgta cggcggctcc ggacccgatc ttttctacca
    34381 gaccgtccag cagggattcc ttttgggtgg tgtcgaagaa tgaatatttc aatgaagagc
    34441 tgccgctgtt cactacgaga attttcatcg gttccgcaac ggaagaaaaa ttcaaatcgt
    34501 aggggtcttc gttacggctg tgggccaagc gtcgtcttct catttcctca atgaactttt
    34561 cgtcttccag aaggcgtttg gtgatgatcc tggtaacttt catcagggtt ttggggttac
    34621 gggacacgac ttgagtgatt acttctcggg gaatcttcac caccttgctg ctccgggagg
    34681 agactacgtt cgctgccgcc ggctcaccgg tgaggatcga catctcgccg aaaaaggaac
    34741 ccctttccag agaggccaat accttctcct ggcctttgtc gtcaggaaga gttacctcta
    34801 cttccccctc ataaacaagc cagagaaaac gcccgatttc gccggtgttg atgattaggg
    34861 ccgcgggggg aaattcgatc agttgggaat tggtggccag aaattcgaga tcttcttttt
    34921 ttaaatcggc gaggatgtcg attgattgga agaacttggc taattccata tcgctgcctc
    34981 ctgacaattg gtcgtgatgc ggttgtagat ctttgcctgg gggtggaaaa tctatcctga
    35041 tatacaggtc ttgtccttca tgctaattta aataattatt ctcgtcaata aaatatactc
    35101 gttttctaaa caactcaaga tgaaaagatc tgggttttat gttaaacaca gggccgtcac
    35161 ctgggcggga gaatgccccg gacgcgaaac ggtaaggcaa acggacctga tcgcagttcg
    35221 gctccggatc gataaaggca cggcccacaa aagataatac tcacgttata tgtcaaaaca
    35281 aaattcacgc aagcacatct ttacccttgg aatcgacgtc ggttccatta ccaccaaagc
    35341 ggccatgatg agggatggca ggctcttcag ctatgctaca acctttacgg gctacaatat
    35401 ggaagcggcc gtgaaaaagg tggtggatgc caccctggcc gaagcctccc tggtgccgga
    35461 gaacctgacg agaattattg ccacgggcta cggtcgtaaa agcgtggccg tggcccatga
    35521 agaccggaca gagatcctct gccacgccgc gggcgcctac tttcaagatt ccgccatcag
    35581 attcgtcatc gacatcggcg gccaggacag caaggccata cttttggacg accggggcaa
    35641 ggtgagcaac ttcacgatga acgacaaatg cgcagccggg acgggacgat ttctcgaagt
    35701 gatggcccgg gcaatggagg taaacctcga cgctttcggc gacctttccc gacaggccga
    35761 cgcaccggcc aagatcagca gcatctgtac ggtttttgcg gaatcggaag ttatttcgct
    35821 gatcgccaag gggaccgccc gggaaaacat cgtcgccggt atccatgatg ccgtcggcgt
    35881 ccgggtggcg gccatggcca ggaggatcgg cttgcggggt cccgtcatga tgaccggcgg
    35941 ggtggccaaa aaccggggag tggtggccgc tctaaaagaa aaactgggtc taaccgtaac
    36001 ggtatcccct tatgcgcaga tcaacggcgc ccttggggcg gcttttttag catggaaata
    36061 acgaacaaga agatcacccg gatccttgcc tacctgtccc gttatctctg aggaacggtg
    36121 ccggcattgc tttcgccgct ttaatctggt cattccagcg ctgccggtgt tagccgcagt
    36181 gatcctggcc ctgttcctca ttcgggatta caagggatga ctgtgatagt gcgagagaca
    36241 tcggacggaa aatagttatc ccggtttgac atgatcagac ctttagccta tatcctatcc
    36301 cgaaataatt ggataaaaat gatcaccggt tcatgaaaag agatgaagaa agtagaaaca
    36361 cataataatc aggatatttc cagacataat acagatgcgc agataaggat gttgctgatt
    36421 tcccggatcg ccatggccac ctttctgttg gccatcttcg tcttcatgca gaaccagtcc
    36481 cccgatttcg ccagcaggat ttccatccct tacttctatg gaataattct cgccatctac
    36541 attctatctc tgttttattc agtcctttcc cggatctctg ccaaccaccg gatgcagatc
    36601 tatttccagg tcatgaccga cgtgattgtc attacggccc tgatctatgt caccgggggg
    36661 atcaggtcaa catacgccgt tttctatccc ctggtgatca tctatgccgt cctgtatctg
    36721 ggacggaatg ggggactgat tgccgcctcc gccgcttcca tctgttacgg tctcctcatc
    36781 gatctcgaat tctaccgcct catcaagcct ttgtcgtatc cctccatgtg ggaaataccc
    36841 gataccgccg gtcatgtatt ttaccgaatc tttgttaata tcgtttcctt ttatctcata
    36901 gcggggttgg ccagctttgt cgtggagaaa gagaggaaga ccagggcgct tctggcggaa
    36961 aaggaatcgg ctttcaacaa gctggacctt ctgtaccggt cgattatcga atccgttgac
    37021 gccggtatcc tgaccataga tatggagaaa aggataaaat cattcaaccg ggcggcggaa
    37081 aagatcaccg gctggagctt tacggaagtg gaaaacaggg ggatcgacga agtattcact
    37141 aactgggaaa gcctgttgga aaacaaaggt agttatgacc ggcaaatcca gggccgcatc
    37201 gaaatgaccg ttacggctaa agatggtcgg aagttgattg tcggttgtct tccttcggtg
    37261 ctccgcgatc acgacgatca caaattcgga gatattttgg ttttccagga cctgaccgcc
    37321 gtcaagcaga tggaggaagt cttggagaga aaccggcgtc tggcattcgt gggggaaatg
    37381 gcggccgggt tggcccatga aatacgtaat cctttggcct cgataagcgg cgccgtgcag
    37441 atgttgcagc gtgaaaccgg ttttagtgcc acggaggcac gcttgatgca gctaattata
    37501 cggagcaagg atcagctcga gggatttatc aaggattttc tcttgttggc caggccggac
    37561 cggggacccc aacaggtggt gcatctcaca gatctcatta aggaagtaat ggaagcggta
    37621 agagttaccc cggactggaa cagcgccatt atactcaggg aagattttga gccgggcctg
    37681 accgtgagcg gcaaccgaga ggaactgcgc caactggtat ggaactttgt actcaacgcc
    37741 gcccaagcca tgccggcggg ggggactctg aagctcgaag cgcggcagcg gtcaaatgac
    37801 gaccggccgg gggtggaatt tattaccgcg gataccggca ccgggatttc cgaatgtgat
    37861 caacggtcga ttttcgagcc tttttttacc accaaagaga ggggtaccgg gttgggtttg
    37921 gctattgtca tgaggattat caacagctac ggcggtaaat tgtccttcaa aagcgtagcc
    37981 ggccggggca gcacatttca agtctggttg ccctggcagg agaatatttc aggaggtttg
    38041 gaaaatggcc aggattctcg tcgttgacga tgatctcgga atgagggatt tgctggagat
    38101 catgctgacc caggaggatt atgaggttgc ctgttgcggg gatccggaca aggccctgca
    38161 aagagttcaa aaagagcagt tcgacctggt cataaccgat cttaagatgc cgaagatgga
    38221 cggaatagag ttcctgaagc aggttaagga agtcaaaccg gaaacacagg taattctcat
    38281 caccgccttc gcttcgggga ataccgccct ttccgcaatg aaggaagggg cttacgatta
    38341 tatagaaaaa aacttcgata tcgacgaact aaagcggata gtccatgaag ccatggaaaa
    38401 aaagggtatc aaaagacagg aggcgcaatt catcaaagac ctggccgatg atgtcgcctt
    38461 cggtggaatg atcggcaaga gcagggagat gctgaaagtc tatgggacta tcaagaaggt
    38521 ctccgaaacg ccagcgaata tcctgatcct cggagaaagc ggcacgggca aggagctggt
    38581 agccaaggcc attcatgaac acagtccgcg gaaagacatg ccctttgtgg tcatcaattg
    38641 tggcggtatc ccggagaatc tcctcgaatc ggagtttttc ggttatatga aaggttcctt
    38701 caccggggcc ttcacggata aggccgggct cttcgaatcg gcaaataatg gaacaatatt
    38761 cctcgatgaa ataggggagt tgccatcgtt attacaagta aagcttttac gtgtcgtcca
    38821 ggagaaaacc tttcggcgga taggtggaac gcttgatatc aaggtcaatg tgcggataat
    38881 ctccgccacg aaccagaacc tggaagacaa ggttaaaaga ggttcgtttc gtgaagatct
    38941 ctactatcgt ctcaatgtaa tccccattca tctgccgccc ctgcgggaaa gaagagaaga
    39001 cataccagta ttgacacgct atttcataga aaaatattcc caagaatttg ccaaggatat
    39061 taagaccatt tcaagttatg ctctggaatt acttatggat tatcctttcc caggtaatgt
    39121 gcgggaactg gagaatatca tcgaacgcag cgtggctttg gagagcacca atatcatcct
    39181 gccggacaat cttttcatcg acagggaaca ggttaaaaat gaccatccac atcccgccgg
    39241 cattcccgaa gtgggtattc agttgaatga agagttggca agaattgaaa ggcagttcat
    39301 cgaaatggcc ctgaacaaaa caaaaggttc aaaaaccaag gcagcggaac ttctccatgt
    39361 gaccttcgat tccctgcgct atcgcatcga aaagctgagg atagaaaatt ccgggtggga
    39421 ttgaaaaaat acttgacaag ggcacccaca tccattaaac gtgattgcca aatagaatca
    39481 aagtgaaacg gaaaatcgac atggcacggc agagaaaatt taatctggct ttctttttta
    39541 gctttaatgg cttttttggg gaggtctgct cgccgcgctc atgacctgat caataacgaa
    39601 tgagccgtgg gcagaccgaa gaggcgccca cggttttttt tggccgtgga gattatcccg
    39661 gccttttttt taaatcgcaa ggggaaaagc gagtaaggag gaagacatga cagcaaggat
    39721 cacaaaagga atcattatcg gcgtgatagt gggattgatg gtgctgggcg tctccggatg
    39781 ggcggcggcg aaggtcgtca aggtgggggc ggcaatcaac atgacgggtc ccgcctcgag
    39841 ttggggccag tttcacgccc ggggacagca ggattatttt cgttacgtca acgaggtcaa
    39901 ggggggtgtc gcgggaaatc gcatcgaaat gcttctggtg gacacggcct acaaggttcc
    39961 cgaagcagtg gcggcggtac gtaagttcgc cgtccaggaa aaggtcgata tgatcgccac
    40021 ctggggcgcc ggcgagggac tggcggccaa gcccatcgtt cagacctaca agatccccac
    40081 catcaactat tccacgagtt gggaaatcct cgaaccaccc atagattaca tgtatcttcc
    40141 cttcggaagc tacaagatgg actgttacgc catcctggat tatatccggg ccgttcatcg
    40201 gggaaacacc atccccaagg tcggtctgct cacctacaat aacgcctatg gtcgatccat
    40261 ccacaaaccc agccgggaat acgcaaaaaa ggtgaagata gatatcgtgg ccgtggaaga
    40321 gtttcccccc aagaccgtcg atctcaatac ggaactcctc aggctgcaga agaagggcgc
    40381 ccagtacgta atcatgcaga tgctccccgc cgccattgtc accaccttca agagcgccga
    40441 ccgcatcggc tacaatccca tgtttttcgg cacctggacc tctacggatc ctgatttctt
    40501 ccgcctgggc agaggcttga tcaaagaccg gctgctgatg caattccccg gcggccttcc
    40561 cgtggaccgc acgcccggaa tcaaggtcat ggaggaatta tggaagcgat acaaaacggt
    40621 caataaattt gatgcctcct attgggaggg agtggtcgtg gcaatgataa tggagcgggc
    40681 attcctccgg gctcacgaaa aattcggcca catcaaccgg gaaacgatca acaaagctat
    40741 ggagtccttt cagaacgaag attttggcgg cctggtgcca aacgtgacct acacgaagaa
    40801 aaatcatggc gcctccttca aggcccggct cgtccgggtt caggagaacg gatcgtacac
    40861 gcccctaacc aagttcttcg ttccgggcaa ggaaaagata cgtttactga agtgagcagg
    40921 cattcaggat gaaagcctat cgcggacagc cagcgatttt tgccgcacgg aaggaagagg
    40981 aacggcgggc ccacctggaa atcaggaacg tctgcctgaa tttcggcggc gtcaaggcct
    41041 tgaacaacat cgatttacag gtgggtaacg gagaactgtt tgccgtgatc ggaccaaacg
    41101 gcgccggcaa gaccagcctc atgaactgca tcaccggctt ctatcgtgcc gtgagcggtc
    41161 agatcctctt caacggccgg gaaatcaccc atcttcacgc ccatgaactg gtaagagtgg
    41221 gaataggcag gaccttccag aatatcgagc tttttcccgg tatgaccgta ttggcgaata
    41281 tgctcctcgc ccggcatctc tattgcggtt acggattccc cggggcctct ctctatctgc
    41341 cgtcggtgcg ccgcgaggag gttcgccacc gcaagttgat tgaagaattg atagattttc
    41401 tggaaatgca ggccatcaga aaaaagaccg tggcatcact gccttacggt ctgatgaagc
    41461 gggtggagtt gggcagggcc ctggccctgg aaccacgact gctggttctg gacgagccgt
    41521 ttgccggtat gaccctggaa gaaaaggagg acatggtacg gtttatcacg gagataaact
    41581 ccgcctgggg ccagacgatc attcttgttg aacatgatat ggccatcgtc atgagcgtat
    41641 cccaccggat cgccgttttg aacttcgggg aaaagatcgc cgaaggcacg gcggaggaga
    41701 tacgccgtca tccggaagtg atccgggcgt atctggggtg aggcagaaca cgatgaccac
    41761 taaattccaa gatgaacaga tgttgacgct gccccgtctg ctctgcaaac aggcggaagt
    41821 cctggggcgc ggcgccgttg ccatacggga gaaggcctat ggagtctggc acacctacaa
    41881 ttgggatgat tatctccgct atgtccgtca tacggctctg ggacttgcct cccttggggt
    41941 ccggcgcggc gagaacgtgg tgttggttgt caacaatcat ccggaatggc tgttctccga
    42001 attgggggca caggccatcg gggcggtgac catcaacctc tttacctcct ccatccccga
    42061 ggaactgcgg acctccctgc agcgcgccca ggcggtcatt gtagtcgccc aggatcagga
    42121 acaggtagac aaactcatcg agattagagc acgcataccc caggtaagaa aggtgattta
    42181 tttagatcca acggggatgc ggtcctaccg ggaggagtcg tggttgcagt catatgccga
    42241 cctgctgagt gccggggagg aaaaagaaca acgggagccg gattattttt cacgggaaat
    42301 caacaggggg aatgccaaag acatcgccct gatgattcag acctccggga caaccggcgt
    42361 ctcgaagctc gccatgcttt ctcatcgcag catgatcgcc atgggtcggc aatggaccca
    42421 ggccatcgcc cttgaacggg gggagaattg gctttccatg tcaccgccgg cctggatcgt
    42481 cgatcagatg tgggggatgg gagtttccct ctatagcggt atgacgatga actttcccga
    42541 gaccccggag accgtgacgg aggacttccg ggaaatcggg ccggcggtga taatcaccgc
    42601 cagccgtttt tgggaagatc tggcctcgaa aatccgggtg aaaatggctg atgccgggtg
    42661 gctgaaacga cggctctttc attggtcgga aatggtaggt gccgccgtta tcgccgctcc
    42721 tggtaacggc aaggaacctg ccgttcttac gagccttctg caccgattgg ctggcttggt
    42781 catctaccgc ccgcttctgg accggatcgg ctgctcagga ctccgtaacg cctataccgg
    42841 cgggcatccc atcagcccgg aagtgatccg tttttttcag gccgtgggga tgaatcttaa
    42901 acagtgctat ggccttaccg aggctggagg aatttttcag gtccagccgg atggggaggt
    42961 aaagccggag accgtgggca aacccctgcc gggcgtccag atcatgatga atgaggatca
    43021 ggaggtgctg gtcaagagcg aggccctttt ttccggctac taccgtgatt ttcaaaacac
    43081 ggaaaaggcc tttcatgacg gctggttgaa gaccggggat gcgggctatc tagatcagga
    43141 aggacatctg gtcatcatcg gccgcaagga agacataatc aaaaacaaga gcgggcaggc
    43201 gttttctccg gatttcatcg aaactcgtct caaattcagt ccttacataa aagaagcggt
    43261 ggtgtttgga gaggccagac cctacatcac cgccatggtc aacatcgaca tggaaaatgt
    43321 gggcaactgg gccgaagagc ggctgatccc ttttaccacc tatctggacc tctcccagca
    43381 gcccgcggtg gaagaattga ttcgcgggga gatcgaaatt atcaacggcc aactgccgga
    43441 gataatgaaa atcagaaaat tcctcctgct ctacaaactc ctcgatgcgg acgacgaaga
    43501 actgactcgg accggaaaag tgcgccggcg tttcatctac ggcatatatc tccccctcat
    43561 cgaggctatg taccgggatg tcgagcaggt ggaaacgaag gggaagatcc gttaccgcga
    43621 cggcagggta ggggagataa cgaccaatgt ccgcatcttg acattgacca tgaaataaca
    43681 ccggaaagga taaatatgga tttttttgtc caactgctgg ttaacggcct gagtgtgggt
    43741 tttctctacg gcatttccgc catgggtttt gtcatgatct tcaagtcttc cagcgtcctc
    43801 aattttgccc acggcgagtt gctggccatc ggggcataca tctttctggt cctggcgacg
    43861 gaggcccaac tgccgattta tggcgccttc atcctcacga tgcttgcctg cttctccctg
    43921 ggattcatcg tggaaaaact ctttctgcgg cccctcatcg gcgaaccgct gatttttgtc
    43981 atcatgctca ccgtgggact gtcagccatg ttcaaaggac tgattctcct gatctggggc
    44041 ggcaacctct acaccttccc ggagttcctc ccggaatggc tggccctgac ctggggcgat
    44101 gtaaccgttc aacccgtcta taccctggcg ctggtggcag gggtgtcctt tctgaccctc
    44161 ttcggtttgt tcttcaagtt ttcctcccag gggatatata tgcgatccgt tgccgacaat
    44221 cagcgggccg ccttgtcact gggggtcaac gtaaagaacg tctttgccct ttcctgggcc
    44281 atcgcggcac tggtggctgg gctttccggc atcattttgg gcatcatcaa cggcgtcaac
    44341 gtccatgatt tgagtgccat cgggttgaag gtatttccgg tggtcatcct cggaggtctg
    44401 gacagcatcg gtggcgccat cgtcggcggc ctcatcatcg gcctgctgga aactcttacc
    44461 ggcggttata tttcgccctc cctgagggac gtggttccct acgtcgtcct cgtcgtcatc
    44521 ctcatgctga agccctatgg cctgttcgga caggtggaaa tagagagggt ctgagatggg
    44581 aaagaggata tttcatccct gcggcaacta tcacgagcga tacgagacac agctcaacgt
    44641 ctatgaaacg gactacggcc gattctggac tgccgttggg gtcatcctgc tgttcacggt
    44701 ggtccctttg gccagttccc cttatctgct gtatgtggtg aattccatcg gtattgccgc
    44761 catcgccgcc atcggactca atatcctcgt cggttacacc ggccagatct ctctggggca
    44821 tggtgccttc ttcggcgtcg gcgcctatgg agccgcctat ctggcgaccc gctgggaggt
    44881 gcccttcctc gttagtgttc ccgccgctgg actgatcacc gccatcaccg gacttgtttt
    44941 cggactgccc tccagccgcc tgaagggact gtatctgacc attgccaccc tggccggcca
    45001 attcatcatt gaatacgtac tcatccactg ggagacggtt accagaggaa ccatggggat
    45061 cactttacct acggcaacga tattcggtct ggatatagcc ggcgatcgaa ccttctttta
    45121 cgtgatcttc gccctgctca tcgcgatgct gtggttagcc gtcaatctca tgcgcaccaa
    45181 atacgggcgg gccttcgtcg ctatccggga caacgatcgg gcggcggagg gcatggggat
    45241 agcgattttc ccctacaaac ttcttgcctt cgccatcagc tccttttacg ccggcgttgc
    45301 cggggcggtt tgggcctact ataccataag tatcaccacc gaacctttca atctcggact
    45361 ttcggtggaa tacatcgcca tggtcatcat cggcggcatg ggcagcatgt ccggagccct
    45421 tttcggtgcc gttttcatca ccggattgaa tgaaatactg agatggggaa ccgatttgat
    45481 gatgaatatg ggctttgtat cgcaaatggg tttgagtatg gccccgctcc gggagtttgt
    45541 cttcggcttg gccattgtcc tgtttatcct gttcgagccc cgggggctgg cggaggtgtg
    45601 gcgcatcgtt cgttccagtt tccgactctg gccgttttct tattgaggag gccgggacat
    45661 gctgttgaag gtcaataata tctccgtagt atattcggat gttatccagg ttctgaaagg
    45721 ggtatccctg aatgtggaaa aggggaagat cgtgtcgctg ctgggcagca acggttcggg
    45781 aaagacctct accctcaagg cgatatcggg tctcttgaaa ccggaaaacg gcaaggtgac
    45841 cgacggcgag atcgtctttc agggaaaggg catccacaac caatctcccg aagcgattac
    45901 ccggatggga atcattcagg ttctggaggg ccgtcaaccc ttcaagtatc ttacgataga
    45961 agaaaatctc cgcgtaggga cagcggtgcg gagggataaa tcatatcgtc aggacctgga
    46021 aatagtctat cactattttc cggccctggt ggcccgacgc cgctccaagg ccggatactg
    46081 cagcggcggg gaactgcaga tgctggtcat cggccgggcg ctgatggcca agccggaact
    46141 tctccttctc gacgagccgt ccctcggtct ggccccgctc attgtcaaag agatcttcga
    46201 catcatcaag aagataaatg aagaacaggg ggccaccatt gtcctggtgg agcagaacgc
    46261 caacatggcc ctccagatcg cccattacgg ttacgtgatg gagaacggca agatcgtcat
    46321 ggagggcaag gccgaggatc tggtcgagaa tcccgatatc aaggaattct acctgggggt
    46381 ggcggcgacg ggtgatgccc gggcctatcg cgatgtcaaa tcctatcgaa gacggaaaag
    46441 gtggttttga ccatcaaagg cggatgtcag acaggaacgt cagttttcgg ctacggacat
    46501 gtcccgcatt ttcaattgga tttcgctgat gccgttccag tgattgaatt gtggggtgaa
    46561 ggcgatgtcc gaggtcgttc cggtggtcaa atgatggaca aaatgcccct tgctgaacca
    46621 gatgccattg caggatacgc cgtcgccgtg gagccgcagc cgcaaatggt tgttgcccac
    46681 cacgttggaa gcggcgacat tcacgttccg tacgcacagg atcggctctg gattacggct
    46741 tccgaaagga gccagcatca ccatttgatt caaaagatca tggttgacat cccggagatg
    46801 gcactgagcg tcgatggagg tttgtgagat catgtttccg ggctgaatat tctccctgat
    46861 cacctcttcc agcaactggc gaaaatcgcc gatgtgttcc tcgcgaatgg agatcccggc
    46921 ggcatattga tgaccgccat aagacagcag cagggagtcg cattttttca gtccctggta
    46981 gatgttgaaa tccagaatgc tccgaccgga gcccttgccg atgccgttac gaatactgat
    47041 gaggatggcg ggtcgataaa aacgatcgac gagtcttgcg gcgacgatac cgataactcc
    47101 ggggtgccag ttggacgagg cgaagacgag ggcggatctg gaaagcggat ccattgtttg
    47161 ggcaatctcc gcgaggatct cgtcgaggat gttcttttcc atggcttgac gcttgcgatt
    47221 gtaaccgtcc agtagtctag ccaattcccg ggcttcgttg agatcgtcgg ttagaagcag
    47281 ttttaccgct tcggatggtg aaccgacccg accggcggcg ttgatccggg ggatgaggca
    47341 gaacgaggcc cggttggagt cgactacctg agtttcgatc ccgcagattt ctttcagggc
    47401 cttgaggccg acccggcgtt cctcggtgat gatatccagg ccgattttgg tgaagacgcg
    47461 gttttcatcg ataaggggtg agatgtcccc gatggtgccc agggccacta gatcgagata
    47521 ttctttgaga ttgggataac tgcgtttgtc ccagaatcct gattcccgca actggcccct
    47581 caggcagata agaaaattga aggcgattcc tacgccggcc agatccttga agggaaagga
    47641 acaatcacgg cggtgcgggt tgattaccgc tatggcgggc ggtaattctt ccggtacttc
    47701 gtgatgatcg aggatgatcg tatcgatgcc ttggctggcg gcgtattgca cttcttcctg
    47761 atcggatacc ccacagtcca cggtgatgat caaaccgatg ccgcgacgct tgaggatatc
    47821 gatggcgttg tggtttagac cgtatccttc cgtgatgcgg tcggggatgt aataatcaat
    47881 gcccggcagc agaggacgaa gaaatctgag gagaacggcg gtggaggtta tgccgtcggc
    47941 atcgtaatca ccgaagacaa cgaccttttc tttcttggtg atggcttgga tgacgcgatg
    48001 gaccgccttt ttcatgtcct gcatgagaaa gggattgtgc aggtcgttta gggaggggaa
    48061 gagatatctt ttggcgtcgt cgatgcagag gatgtttctg ctcatgagga ttttggatat
    48121 aatcggcagg atgccaagtt cccgggccaa atgttccgtg aggtcctctt tttccaggga
    48181 aaaacgccac ctggttaccg gcaacgactg tttattcctc aattttagat acatattcct
    48241 tttattttga ttctgtatca tagacttatc agtcgtaatg tcaagcagga aatttgcttt
    48301 tttgttgaca cggcagcggt cactggcata ctaagaagtc atgaaaactg tggaaattga
    48361 aatgtcgcgc aaaaatctgg cgttgttcca gttcctgttg gaaggatacg ggcgggtggc
    48421 cgccgtgacc accatcgatc ctcatatagc tcgagtgcgc attaccatca tgccggattt
    48481 caaagaagaa gtattcgaga taattgcacg gattgccaaa attgtcgcct gccggcggat
    48541 ctcctgaccc ggatctggaa cggatggtga acattgagct ccgctttcag ggaggaaaag
    48601 atgaagcggc tttaccggtt gataaacgtg attgttatac ttcccttgct catgggcatg
    48661 agcaatctgg aaggcgaatc tcctgcccgc atccccgtgc cggaaaagaa attcaaggcc
    48721 gcgttcatcg atcagaccga cacctatacg gatgtcagcg aggccagtat cgacgggcat
    48781 gcctttctcg ctgggaaaaa aggggaagga aattacaccg tggcgtttga gaacatccat
    48841 tatatcgatt atcttatgaa aaatgatatc ctcatcgccc aaatacaaat gagggacggt
    48901 gccgtcctgt ccctcaccgt aaaaaagaat tcccatgcct ttggtaaaac gagctacggc
    48961 acctttcaaa tccgcctggc ggacctgaaa aggatgacga tcctgggtcc gcagaaatag
    49021 taccgcatta ggaggcctca catgctgatg atgacccata cctggcttct cgacagctat
    49081 cttaaggaaa agcggctcag cagcgattat ctggatctgt tcatctacaa tatctgtccc
    49141 gatctccttc ctcttcacaa agggattacc gcatcggaaa cacacggcat ctcccgtttc
    49201 accccgctac cccagaatcg ccgaaaaaat gccttcgttc aattccatct catggtggat
    49261 gacttatccc accacgggat gattcccacc gagcccgtcc gggaattcaa ccccgattcc
    49321 caaggctata catatttgaa gggtaaacca ctggtagaac cactgcgtga tatttatgaa
    49381 tttcagggca cgcccatccc ctatgcccag gccgcctacc gctcccacat ggtaatcgag
    49441 atgaccttcg atcttgccat gcatggtgcg gaaagcagag agggcgaaag gttgatcacc
    49501 cttatatgcg atgccatgca cttcaccgtg gaggaaaaac ttacggaatt cagcaccgac
    49561 atcgcctggc tgtatggcat gtcgccggcg acggtggcgg aaaccatgcg aaagggggcg
    49621 gcgttttaca atcgggcgcg gatcaggagt ttcatgagca tggaaggcag aattcagctc
    49681 tttgccggca aattcggtat ttcccgggag gatgatcagg cgctggccat gttggcggag
    49741 gtcatgaatc agggaatgga gttggtgagg gattacgatg acttcatgaa gccggccctc
    49801 gcggagatcc ggagcgccgg cttcaatccc gaatactgat caaaccgcct tccggacgaa
    49861 tccggatcta aggcagcggg tacagatctt gatgcggcgc accgccccgt ttttgtcgtc
    49921 caaggcgcga atcttctgga ggttgggccg ccaaacccgc tttgtcttgt tgttggcgtg
    49981 actgacgtta tttcccacca cgggtctttt cccgcatatt tcacatactc tggccataaa
    50041 aactccttgc aaacaatagt ggcgtggttg ctacaacaac aggggaaaat atgcaagcct
    50101 tttttcaccc ttcgtttttc atcaccaggg gttggccggt cgattcgatt acccgcatcc
    50161 agatatttcc ggtcggatca acctttttcc ccgacataac ggcccgcaga ggaagatgca
    50221 cgtattcccc gcggaaaagc ccgaccagca tcccggtctt gccgctcatg ccggcatgga
    50281 cggcgtattg tcccagggcg ccgcagtaaa tgctgtcccc ggcattggcg gggacgctac
    50341 ggatcatgta gctggggtcg atatacttga cgtttaccgg aagaccatca tttttgaaat
    50401 gcccctcgat ggcgcacttg agatacaggc cgatatcgaa aagcttcaga tttcccgagg
    50461 cgtcagtctc gatggtgtca tcttcccgga agaagaactc ctggccggca ccttccgcca
    50521 ccagaatgac gcaatggagg cggctgcgca aacgctcttc cagggccttc aaaaaacctc
    50581 tttctccgtg aaggtcgaaa ggtacttcag gaatgagaac gaagttggcg tcgttctgag
    50641 ccagagcggc atgtacggcg atatgccctg attgccggcc catgagcttt acgagaccga
    50701 tgcccatggg tgcccccttg gcctcaacat gggcggacct aatcgcctga acggcctcgg
    50761 aaatagccgt gtcgaaaccg aaggtcttgt ccacgagatt caaatcgtta tctatggtct
    50821 tggggatgcc gatcacgctt atatccagtt tcctccgggc aatctcttcg gtgatcagtt
    50881 ccgtggcccg catggtgccg tcgccgccga tacaaaaaaa tatgtcgatg ttcatccgct
    50941 tcaggacatt gacgatctca ccgatgtcct gttttccccg ggatgaagaa aggatgctgc
    51001 cgccgacagc gtggacatcc tttaccagct ccggcgacaa ctctatcacc tcatgtccga
    51061 aggcggggat aagcccctga aaaccatatt ttattcccac aatgttttgg acggcgtaac
    51121 gatgatagag ttccatgacg atcgcacgga taacatcgtt tattcccggg cagagaccgc
    51181 cgcaggtaac gatggccgcc ttggttttgg gcggatcgaa gtagatcatc cggcgggggc
    51241 cggcgacttc gatggacagc ggcggctgga ggtcccaagg atgagaggaa agatgacgca
    51301 gatcgttgtc atagatgatg cgccggtcgt cgggggtgaa atagctgacg gttatcggtg
    51361 agggaatggt ggcactcccc agggctggta tttcaaaatt caggtcgctg gtcgtcatca
    51421 ttattcctcc ttttccccgc ctcctgtccg aataatgcat tcaggacgta acaccgcgca
    51481 cggaaacggc atcatctcga cggtaacggc cggggatgag tcatcattcc gagagagggg
    51541 gattgtgctg cgcccggata tctcccccgc ggtttccttt gagcagcacc ttgcggcctt
    51601 gcacttccag gcgattttcc gcataccact gaatggcccg agaataaatt ttatgctcct
    51661 cctggaggat ccgggcggcc agcctttctt cattgtcttc atcaaaaacg gggaccacag
    51721 cctggataat gatcggtccc gtgtccacgc cgccgtcggc aaaatggacc gtgcagccgg
    51781 aaaaacggac accgtagtcc aaggcctgtt tctggacgtg cagacccggg aaggcgggaa
    51841 ggagcgccgg atgaatattc atgatccgca tgggaaacgc ggcaaggaaa cttttcgtaa
    51901 ggatccgcat gaaacccgcc atgaccacca gttccacgtc ataggagcgg aggacgtcga
    51961 tcaaatgacg atcgaaatcg ttccgggaag tatagtcgcg atgatctacc acgatcgccg
    52021 gaacattgtg gttccggcat cgctgcaggg agaaggcgtc ggcattgttg ctgatcacga
    52081 tgcttatttg tgcatccaga cgtcccgctt cgatgttgtc gatgatcgcc tggagattgg
    52141 agccgccgcc cgaaacgagg accccgagtt tgagcttgtt tttcatgggt ccgtaaattg
    52201 aactgacgct tcttgctccg agcgtttttc gatgtgaccg atgcagaacg gctgttctcc
    52261 catcatggcg attctctcca tgacttcatc ccgatctcct tcggatacga cgatcatcat
    52321 ccccaccccc atattgaata cgcgccacat ctccatttcc gccactttgc ccagatcccg
    52381 caagagtgaa aatatgggcg gcaccggcca gctttgccgg taaacaacgg ccttgcacgg
    52441 tcccggtacg atgcggggaa tattgtcgag gaaaccgccg ccggtaatat gtaccaaacc
    52501 cttgatatgg aagctcttta tcaatttgag cagggctttg acgtagatcc gggtgggttt
    52561 gaggagctct tcgccgatgg aaacatccag tccggccggc cggtcggctg cgctcagatt
    52621 ggccatgtcg aagacgatct ttcgggccaa agaatagccg ttgctgtgca ggccgctgga
    52681 ggccagaccg atgatggtgt cgccgacgcc gattcccgat ccgtcgatga ttctatcgct
    52741 gttcacgacg ccgacgcaaa atcccgccag atcgtattcg ccctccgggt aaaaaccggg
    52801 catttccgct gtttccccgc cgatgagggc acagccggcg tcgaggcatc cccggacaat
    52861 gccccggacg atgtcgattc ccgtctcgat gccgatcttg ccggtcgcca ggtaatcgag
    52921 gaagaacagc ggctccgctc cctggacgat aacatcgttg acacacatcg ccaccagatc
    52981 gatgccgata gtgtcatgac ggttcatcat ctgggcgatc ttgagtttgg tccccacgcc
    53041 gtcggtggac gagacgagga cgggatcggg ggtccggttt agatcgagat gaaaaaggcc
    53101 gccgaaaccg ccgatccgcc ccatgacctc tttacgggcg gtgccgctta cgagcggttt
    53161 gattctctcc acaaagaggt tggcctgatc gatatccacg ccggcctgcc gataggttgt
    53221 cgtcatcatt atcatccttt agtgatctgg actgcatgga aaacaaagct taatgttcac
    53281 aaagcgaagc ttaatgcgtg caaagcggag cttgatctcc ataaagtaaa gcttaatgtt
    53341 catataatgg aaaaattgcc aaccgggctg gtctgcgatt tcatagctca atacgtcggt
    53401 aaagtcaatt gatgcctgct taccagcggg aatttgtttt gacaaatgta cgccactatt
    53461 ataaaaacgc gaaaacttaa atttgggata tcaggccatg aagaagatca cgttgggatt
    53521 gataggcttc ggaaccatcg gctccggggt ggtaaaattg ttgcgggaaa acggcggcct
    53581 tatcgagagg cgcctggggg cccgtctttt tctaaaaaaa atcgccgatc tggatattac
    53641 cacgccccgg gtggttcccg tccctccgga gctgatgact accgacgcgg cacagattat
    53701 cgatgatccg gataccgata tcgtcatcga acttatgggc ggctatgaac cggcgcgctc
    53761 cttcatcctg gcggccctgg caaatggaaa acatgtcgtc accgccaaca aagccctgct
    53821 cgccacatac ggtaacgagg tgttcatggt cgcggaccgc catgatgtgg atatccgttt
    53881 cgaggccagc gtcggcggta caatccccat tgtgaagacc ctcaaggaat cgctggtagg
    53941 aaaccagatc gattccttca tggcgatcat gaacgggaca tcgaactata tcctctccaa
    54001 gatgaccgat gaagagaaac ccttcgatgt cgtcctgaaa gaggcgcagg cactgggttt
    54061 tgccgaagcg gatccgacgt ttgatattga agggatcgat acggctcaca aactggcgat
    54121 cgccctctcc ttggcctacg gaaaacgggt ggatatcgac gagatctatc gggagggaat
    54181 atcgggaata agcatgcaag atctggaatt tgcaaaagaa ttcggatacc gcatcaaact
    54241 gctggccctg gccttctccc gaggggactt ggttgaggcg cggattcatc ccacaatgat
    54301 tcccgttgat gcgatgctgg ccaatgtaaa cagtaatttc aacgcgtttc atattatcgg
    54361 cagttcatcc ggaccggtat tcctctacgg tcaaggggcc ggcatgatgc ctacggccag
    54421 tgcggtaatc agcgacatca tcgacattgc ccgagggatg ctccacggag tcagccgcct
    54481 cgttccctac cgttccctgg gcgaagacat aatcgatgac atccggctta tcccctttcc
    54541 agagatccga acgaactatt atttccgttt tatggccgcc gacaaggctg gggtactgtc
    54601 gaagatcagc ggcatccttg gagaaatgaa catcagcatt gcctcggtca ttcagaaggg
    54661 gaggaagaag gatggagcgg taccggtggt aatgacgacg cacaaagccc gggaacggga
    54721 cgtccgggag gccctggcgc ggatcgatcg cttggacgtg gtccatgaac cgacggtgtt
    54781 gatacgcatc gaggatgaga ggttgtgatg aagtacatcg tcttgcttgg tgacggtatg
    54841 gccgattatc ccctggagcg gttgggaggc aagactcccc tggagacggc acggaccccg
    54901 caaatggatt ttctggcccg ggaggggatc ctgggcctgg tggacactat ccccccaggc
    54961 ttcaaggcgg gaagcgacgt ggccaacctt tccgttcttg ggtatgaccc cgcaacctgc
    55021 tattcgggac gcgggcccct ggaggcagcc aatatgggcg tggcgctcgg accccgggac
    55081 gtggccttcc gctgtaatct tattactctt ggcgatggcg gggattccac catggacgat
    55141 ttcaccgccg gacacatcac cagcggtgag gcggcggaga tcatcgcctc actgcaagat
    55201 accttgggaa atgatgagtt ccagttcttt ccgggagtaa gttatcgcca tctcttcgtc
    55261 tggcgcaatg gtgtcgcgga gatgcagacc acgccacccc acgacatcac cggacaaaag
    55321 agcaatgctt acctgcccgc gggaccgggg agccggaccg ccattgacgt gatgagtcgg
    55381 gctaaagtca tcctgacgtc ccattcggtt aatgagaccc gaaagcggga gggaaagcga
    55441 ccggccaatg ccgtctggct gtggggtcaa ggccgggtgc cgcaaatggt gaagcttacg
    55501 gatcgatacg gattccgggg cggagtgatc tccgcggtag atctcctcaa cggcatcggt
    55561 gtctatgccg gactgcaacc gctgcgagtg gaaggggtca ccggctatat agataccaat
    55621 taccgcggca aggcggagcg agccctggcg gcgcttcagg atctggattt cgtctttgtc
    55681 catgtggagg cgccggacga gatggggcac gagggcaatc tccccggaaa aatcaaggct
    55741 atcgaggact tcgacaatct ggtggtaggt ccggtcctca aggggatgcc cggcctcggc
    55801 gcggtgcgca tcctggtcct gagcgatcat cccaccccga tcagcatccg tacccatgcc
    55861 gctgatccca gccccttcgc cgcatactca accgttgaaa aggaaaaccg gccttccggc
    55921 cggagctacg gcgaggcaac ggcccgggaa accggcctgc tggtgacccc cggcagcgac
    55981 ctcatggatc tcttcatctc tccaggaagg ccattctttt gataaaccgc actaaaatcc
    56041 gggtaatcta cgccgataca gatgccatgg ggatcgtcta tcatacgaac tatataaagt
    56101 ggttcgaggt ggggagaacg gaactattcc gcgacttggg aatcatctat gtggaatggg
    56161 ccacggaggg catcagcctc cctctcaccc gggcctactg ccattatctc cttccggccc
    56221 gctgcgacga tgtggtttgg gtggagacgg agatcgctta cctgaaacgg gcgagcgtga
    56281 aattccgcaa cataattctt aatgaagaca aaaagcggat actggcgaag ggatataccg
    56341 tccatgcctg cctaaaccgg gaaggcaaga tcagccgcat tccggagaaa atggcggcca
    56401 tgatcggcag aggtataaag actgaaacag gagagtaata tggcaagcaa gatgaataag
    56461 caggagatac tgacgccgga caaatttcag gtatttcttg aaacagcgag agattacatc
    56521 ttgacccaca aaaggaacat agcggtgggg acggcggtcg tgacattgat catcgccatg
    56581 gtcgccggct gggcctactg gcgccagacc gaggagacca aggccgcgaa tctctacaat
    56641 caggccgtac tgaacaattc ccgcctggcc gtgatgaacc aggatgtggc agccaccgta
    56701 aagccgtttc aggaagtgac taacaagtat cccggcagca aggctgccat tctttctcaa
    56761 taccggatcg gcaacgctta tcttaacgcc ggaagaaccg atgaggcgat aaagttcttc
    56821 caggcatatc taaaggggaa aagcggtgat aacgagttga cggccctggc ctacaacggc
    56881 ctcggctact gttatgagac catgggcgac ttcaagcttg ccttgaacaa ctatgagagg
    56941 gccctgcaat cccgggcggg cggacccttc gccggcgata tcctcgccgg tatggcccga
    57001 agctacgagg gaatgcagga caaggacaag gccagggaat attaccggca ggccatggag
    57061 aaggtgcagg atccaacgct gaagatgctg atcggccgga aattggcaat gctgggttaa
    57121 tcgcaaatgg ggagatagga atcttatgaa agtattgatg tcaggaaatg aggccatagc
    57181 cagaggtgct tatgaatgcg gagcgaggtt cggcgccggt tatcccggga caccgagcac
    57241 tgaaattctg gaggaattct caaaatacga aggtgtgtac gccgaatggg cccccaatga
    57301 aaaggtagcc ctggaggtgg gaatcggggc ggccgtggcc ggggcgaagg ctttggtcac
    57361 catgaaacac gtcggagtga atgtggccgc cgatcccctg ttcacggtta gctacacggg
    57421 gaccaacgga gcgctggtcg tcgtatccgc cgatgatcca tccctgcaca gttcccagaa
    57481 cgagcaggac aaccgcaatt acgcgaagtt cgcaaagctg cccatgctgg agccggcgga
    57541 cagcgccgag gcggggaggt tcgtcaagat cgcctttgaa atcagcgccg ctttcgatac
    57601 cccggtattt ctccgctcca ccacccgggt atcccattcc aagtccgtgg tcgttaccga
    57661 aacacccgtc ccctatgagg atcgaacgga gttgcggatc aacccttcca agtacgtcat
    57721 ggtccccgcc atggcccgta taaggagagt ggaggtggaa aaacacctgc aggccctacg
    57781 ggaatttgcc gagacctttc ccgaaaaccg gatggagatc aacaataccg agatgggcat
    57841 cattacggcg ggcatgcctt ataactacgc caaggatgtc ctgccaaact gctcctattt
    57901 aaagctgggc ctagtccatc cactgccgga aaagctgatc cgcaaatttg cctctctagt
    57961 taaaaagcta tttgttatcg aagaactgga tcctttcctg gaagaacaga tccgggctat
    58021 gggaatcgaa gtaacgggca aggaactctt ttcctatacc aatgaatttg atcccggggt
    58081 catcgccggg gcaatgatga agaaaaccga cgcggcggta aagttgccgg aggtcgccat
    58141 tcctccccga ccgccgaatc tctgccccgg ttgcccccat cggggccttt tccatgtcct
    58201 ggggaagatg aagacgacat tcgtcgccgg agatatcggt tgttacaccc tttcgttcat
    58261 gaaacccctg gaagggcttc actcctgtgt ctgcatgggg gccagcatcg ggatggccca
    58321 cgggatgagc aaggccttgg gggaaaaggg aaggggaaag gtcgtgggcg tgatcgggga
    58381 ctccacattc atccattcgg ggatcacccc cctgttgaat ctggcctata atcaaggcga
    58441 tgccgtcatc gtcatctgcg acaaccggac cacggccatg acggggatgc aggaacatcc
    58501 cgccaccggc tacaccctcc agggggcaaa ggccaaagag ttggattttc aagccttggc
    58561 tgcggtcctc ggcatcgaca gcgtccgggt catcgatccc tacgacctaa aaaacacccg
    58621 gaccgtcatg aaggaggaac tggccagaag cggatcatca ctggtcgttt cccggcgttc
    58681 ctgcgtactt ttcaaacgcc ggtacacgga aactcgaaag ccactccggg ttaatgtgaa
    58741 taaatgtatc ggttgccgga attgtctcga tttgggctgc ccgccgatcg catggcggag
    58801 cttcaaggat caaccggcga acgagattat aaagaacgcc aaaaagcagg aaggaaattc
    58861 atatatcgat gccgatttat gcacgggttg cacgctctgc cgtcaggtgt gcaaggtagg
    58921 agccatcgag gaggtggaat gatccatgga caaaaaaggc aaaaaagcgg gtaaaaatat
    58981 aaccggccgg gtaatcagcg ttctgatggc cggagtgggc ggtcagggga ttctccgtgc
    59041 cagtgatatc ctgtgccggg tcatcatgga ggcaggcctc gatgtgaaaa agagcgaggt
    59101 gcacgggatg gcccagcgtg gcgggtgcgt gaccagccat gtccgttacg gacaaaaagt
    59161 ctattccccc atcgaacgca aggggaatgt ggagatcctc ctggcctttg agaagcttga
    59221 ggcgctgcgc tacctagact atctcaagca ctcgggaagg atcatcatca acgaagtgga
    59281 aatctatccc ccggcggtca atctggggtc gatgacctac ccggataatg ccgttgacct
    59341 cgtccgccat aaattttcca ccgtgatggt tgtcaatggt cgtgacctgg ccgcggcgtc
    59401 aggaaatatc cgcacggaaa atacggttct cctcggcgca ctgtcgcaat ggctcgattt
    59461 cgagctcgac atttgggagc gagtccttaa ggagtctttt cctcccaagg ccctggacgc
    59521 caatcttcag gcctttcatt tgggacggga ttatcgggaa gggtagggtg cagttctgga
    59581 agaaagacaa tcgtcctgaa accagcgaag ctggttaatg ttcacaaacc ggcgaagctg
    59641 gttaatttgc ataaaccgtc aaagctcatt tatgttcata aatatcaggt taaatcttga
    59701 caacccggcc ttcgttggta agttagcgat aataaatctc cagcggggaa agtaaaagaa
    59761 tggcagaaga ctattatcag ttgttgggtg tggacaagaa agcgagtgcc gaagaaatca
    59821 agaaggccta tcgaaaactg gctttcaagt ggcatcccga ccggaatccg aacagcaagg
    59881 cgcaggccga ggaaaaattc aaaaagatca gcgaggctta tgccgtattg agcgatcagg
    59941 aaaagcgcca gcaatacgac atgtatggtt ccgccgatca gttccggcag aaatacaccc
    60001 aggaagacat ctttcgcaac tttgatttga atgatctttt gcgtagtttt ggctttgatt
    60061 ttaccggagg agggggaaga accaaaggct tctctaccgg tagccggcgc cggggcggtt
    60121 ttcagcagtc ctttgatccc tttgccgatc tgttcggggg cggcgggcaa tcgtacggcc
    60181 acatgccgcg gcaggggcag gaccttcaat acaacctctc aataacctta gaggaatccg
    60241 tccagggagt ggaaaagaaa cttgccctgc agcgtgagga tcgggtcgat gagatcaacg
    60301 taaagatccc ggccggaatc ggtgccggca aaaagctgcg gctggtgggc aaaggggctc
    60361 ccggatttga cggagggccg actggtgatt tgtatctcca catcaatatt attccccatc
    60421 ccctgtttgt ccgggacggc aatgacctct ttgtggaaaa aacgatcagt tttacccaag
    60481 ccatacttgg tacgaccatc gatgtgccga cgattgacgg tggtacgaaa aggttgaaga
    60541 ttccaccggg gacccagaac aacaccaaaa taaggatgaa aggttacgga attcccggtt
    60601 tgaagggtgg attaaagggt aatcagtatg taaagatcaa catccaggtt cccaagacat
    60661 taacggagcg tcagggtaaa ctcgtccgac agctagccga ggaaggtctg taagaaatat
    60721 tcaaaaatat acccgtattc tgaatgtttt tcaaacggag agaaaagaaa aaacgggaag
    60781 aacaatcgtc atattaacaa taatatctta gacttatcct ctgccggtgc accggcgaag
    60841 atttcggcgg ctccagtaac tgtcaaatat ttcatgacgg gtatgtgact taatggttaa
    60901 tatttaatct taacaaaata gtcgcgtaaa aaaccctcat gcgccgtggg acaccacgaa
    60961 gcatgaaaat ggcatctccc gcattcagtc aaaatttgtc aacttcagct ttggaaatga
    61021 cttttgtgcc tgaataatcg attattgaat gtcgcataag atatgttatg taaactttat
    61081 tccgaaaatt ttatttttcc ccatacccca agtttatcgc ccagaataat gacgattccc
    61141 tgcacccctt gtatggctcc cccgacctcc agggctttct tgatgttctc ggcccggcgc
    61201 accaaattac cgatctggga agcggcggca tcggccaggg ctgctgagga tgaaaggaca
    61261 caaaccgcat ctgcctttcc aaaactcaaa gaaggaccca ccgtcgccga tgaggtgcag
    61321 attcccaacg gcatgtcttc cggggagatt tccagggtca gcttcccgct caaaggggat
    61381 tgaccggcga aaacggctac ccgcctcccg gcggcagtat gcatgaagat gtcgccgccg
    61441 ttttcgacaa taatgttttt tgtctgcgcc aacaaatcac gacccacata gtccgccacc
    61501 gcaccggcaa cagcagccat aggccccacg ccggccgtat tagccgccgc cagcatcgtc
    61561 cggacaatgg ggggcgccag cgcatcgttg ggcagcggtg tcaaggagtg gcgaaaaacg
    61621 ggatattttt tgatatatga ctcgagatga cttcggtgct tgaggatgga tgtccaggcc
    61681 tgctcggtaa gatcggtgtc agcggcaata aagagatcag tttcccgcac cgcgacctga
    61741 aaggtaaaca aacctgaggc ggtaaccgct tgtcggtagg tgcgctcact atattccata
    61801 aaagcgaata cttccttacc cttaatatcc ttatgaatat tgaacaatta tgtgtttaat
    61861 gaagaaataa catcaacggt agaaggagga ctgggggcca aggatcagac gggcggcctt
    61921 taaaagttcc gatgtcaaat gaaagcggca atcattgccc agatagacaa cagtttcgct
    61981 caccccgttg agcagatgga tatatccatc agaagttcct ttatatttat tcgacaagta
    62041 tttaatattc aatatattat tatcgtcagc agagttctgg gaatcgatgt agaaatggac
    62101 ggaggtatag ggagagacat ccggggcgtc ctgaatgagc gttatttcat tggcaatgat
    62161 tttgacgttt tcctccgaga cgtccgtttg tccttttatc aacagcggtt cgtcgctttt
    62221 cagaagttcc ctgccccgct ggtaaacatc ggcgaaacag atgatgttca tggagccata
    62281 aaggtcctcc agggtaacat aggccatcac gtccttccgt tttgtcgtca gctcccgaat
    62341 gccgctgacg atcccggcaa tgatcacgtc ttctttatcg cgccgttcag acaggttttc
    62401 cgagttggca ttggtgatga aactcagctt ttcctgaaaa cgcaggagcg gatgaccggt
    62461 aatgtaaaag cccagtgcct ctttttcata cattagcagt tccttatgct cccattcccc
    62521 cagatcggga atctcttcat tgtgagcgcc ggccctaccc tgccccacca cctccaggcc
    62581 atccaaaagg ctcgtctggc tgctggactt cgctttgcga agtttctgag ccgtattcat
    62641 gatctcttca aatgctccga aaagggttcg gcgcttgtgg ccaagagagt cgaaagcccc
    62701 gcattttatt agactatcaa tgacccgctt gttgaccttt ctgagatcga cacgccggct
    62761 gaaatcatga aaggatttaa attttccccc tttgtgccgc gcctccagaa tcgcctccac
    62821 ggcgccgagg cccacattct tcaccgccgc caggccgaag cggacatggc tgccgggaac
    62881 ggtaaaatca ctccgggatt cattgatgtc cggtggcagg acactgatgc ccatctcctt
    62941 gcaactgttg atatggcgga tgatcttgtc acggttatcc ttttcgctcg tcagcagcgc
    63001 cgccatgaac tcctcgggat agtgagcctt gaggtaggcc gtctgatagg agacgatggc
    63061 ataggcggtg ctgtgggatt tgttgaagcc gtatttggcg aattccttca tctgctccca
    63121 gatgcggatc gccttttcct ccgggatacg gttttgcttg gccccggaga taaatttcgg
    63181 catctccttt tccatttcct gatctttttt cttgctcatc accttccgaa gcgtatccgc
    63241 ttcggccatg gtatagcggc caatggcgct ggcaatctgc atgacctgtt cctggtagag
    63301 aataatcccg tatgtttcct taagaatatc ctttagttca ggaattacat agctgatcgg
    63361 cgttttgccc agcttccggg cgatgaagtc gggaaccata ttcatgggtc ccggccggta
    63421 cagggcaatc agggcgatga catcctcgat gcagtccggt ttcatcccga cgaggatctc
    63481 ccgcatgccc gagctttcca actggaatat cccatcggtg ttgcctcgct gcagaagttc
    63541 atacgtttct ttatcatcca gagacaagga atcgatatcg atatctattc ctctgttctc
    63601 tctgacgagc tgcagggtgt ttttgataac cgtcagggtt ttcaaaccga gaaaatcgaa
    63661 tttcgtcagg ccgacggcct ggatatcgtt catggagaac tgggtggtca ccccctcgtc
    63721 cttggggccc ttgtagaggg gcacgcggtt gtccaggggg acatcggaga tgacaacgcc
    63781 ggctgcatgg gtggaggaat ggcggttcag cccttccagg gcctgggaat aggtcagcag
    63841 tttctggatg cgctcgttct ttttcgcctc ttcctgaagt cgcggctcca ttttcagggc
    63901 gtcggcaagg gtgatgttca ggacattggg aacgagtttg gcgatggcgt ccacctctcc
    63961 ataggggata ttcatggccc gacccacatc cctgatcacc gccttggcca gcatcttgcc
    64021 gaaggtgata atctgcgcca cattgtcccg gccgtatttt tccgttacgt aacggattat
    64081 ctcgtcccgg cgttcctgac agaaatcgat gtcgatatca ggcatgctct tccggtcggg
    64141 attgaggaat ctctcgaaga aaagcccata gcggatggga tcaaccttgg tgattccgat
    64201 agcgtaggcg accaggctgc cggccgctga acctcggccc ggacctacgg gaatgccgtt
    64261 gtttttggcg tagttcacga aatccgcgac gatgagaaag taaccggcga agcccatgtt
    64321 tttaatcatg atcagttctt cggagaggcg cttttcatac ctgcgtaccg tatcttcatc
    64381 accggtggta ttgatggccg gcagcagcct ggcaagtccc tccttggcca gcgcggccag
    64441 atgctcatca aggctccgct caccatccaa atggtaacgg ggaagataga attgcccgaa
    64501 ttccaaggat aggttgcact tttccgccac aacgaccgta ttggacagcg cttcgggaca
    64561 gtaggagaaa agttcctgca tctccgcggg ggagcggaaa taaaactggt ccgtctggaa
    64621 ccgcatccga tcttggtcct cgatggtctt tcccgtctga atgcacagga gaatttcatg
    64681 agcctcggca tccgcacgct tgagataatg acagtcgttg gtcgccacca ggggcaacga
    64741 caacttcttg ctgatttcca tcagtcctct gttggcggtt gtctgctccg ggatgccgtt
    64801 ttccatgatt tccagataga aattatccgg tccaaaaagg tcgcggtact cggcggcggc
    64861 ccgaagcgcg gcgtccatat cctgtctgat gatgtgggtg gctatttccc cgtggaggca
    64921 ggcactcagg ccgatgagcc cctcgtgatg ggcggcgagc agttctttgt cgatccgggg
    64981 ccggtagtag taaccttccc ggtagctgtc cgaggataac ttcaggagat tattgtaacc
    65041 attcatgttt tttaccagca ataccagatg ccgggaattt tcgccgctgc cgggcgtatt
    65101 cttgtcggtc ctccgaccgg gagcgacgta catctcgctg ccgatgatgg gctttattcc
    65161 gtggcgatag gcctgctgat agaagtcaat ggctccgaag acattgccgt gatccgtcat
    65221 ggccaccgcg ggcatctgat attcctttgc ccgcttcagc agatcctcga tgcggattgt
    65281 accgtccagg aggctgtact gagtatggac gtgcaggtgg acgaacgggg cgtgcttcat
    65341 agtcaatcca accagtagtt gggtgcttct ttcgtgatga tcacgtcgtg gacatggctc
    65401 tcccggagtc cggcggaggt gattctcacg aacctcgcct tttcatgcat ctcaggaatg
    65461 gtccggcagc ccagatagcc catgccggcc ttgagccccc cgatcaattg gtagatgctc
    65521 gccgacagag agccccgaaa tgggactcgt ccttcgatgc cttccggcac gagcttcaaa
    65581 ttgctctcca tatcatcctg atagtagcgg tcccggcttc ccgatttcat cgcctctagg
    65641 gagcccatgc cccgatatac cttgtagctc cgaccttgaa agaggatcgt ttcgccaggg
    65701 ctctcctccg tgccggcgaa gaggccgccg atcatcaccg tatgggcgcc ggcagccagg
    65761 gccttggcga tatccccgga gaactttatc cctccgtcgg cgaccacagg gataccgcga
    65821 cgggagcaaa ccttgaaagt ttcgcgaatc gccgtcatct gcgggacccc aaccccggcc
    65881 accacccggg tagtacagat cgagcccgga ccaacaccca ccttcacggc gtctacaccg
    65941 gcgtcgatga gggcttccgc cccctccgcc gtggctacgt tgccggcaat cagttcacac
    66001 ttggggaaat taagcttcgt gtcccggatg gcatttagga cgccccggga atggccatgg
    66061 gaggtgtcga tgacgatgac gtcggcgcca gcggcgatga gggcctggat ccgctcttcc
    66121 cggtcgagga tgcctaccgc ggccccgaca cggaggcgcc ccagggaatc cttgctggca
    66181 ttgggatatt tacgtatttt ttcgatatct ttgatcgtta tgaggccttt gagattgtac
    66241 tcgtcgtcca cgacgaggag tttttcaatc cgatgcttat ggaggagctt tttagattct
    66301 tcgagggaga tattggaggt cactgtcact aaattatcct tggtcatgac attggccacc
    66361 ggctgttcga gatcggtttc aaagcgcaga tcacgattgg taaggatgcc cacaagtctt
    66421 ttgcccttta cgaccggcac accggaaata cggtattccg tcatgagatg gagggcctcc
    66481 tgaaccttct gttccgggga tatggtgatc ggatcgacga tcatgccgct ctccgatttc
    66541 ttgactttgt ccacctcgat cacctggcgg tcgatgctca tgttgcgatg gatgattcct
    66601 attcccccct cctgagccat gcagatggcc gtcttggatt ccgtcaccgt gtccatggcg
    66661 gcgctgagga taggaatcct caggattatg ttattggtaa gatgcgtgga agtatccacg
    66721 tctttgggca atatggacga ttcggcgggg acgaggagca aatcgtcgaa agtaagggct
    66781 tcaccgatgt tatcggtccg catgtgaata cctccatcaa ttttattttc ttttaagatg
    66841 tttcttcagg acttcgtatc gagatcgtaa atccggccgt tgatggggag gtaaaaagcg
    66901 tttctcccgt catcatactc gatatgctcg gcaagctggt agtaggccgc ccgggtgaag
    66961 cgcgccggaa aagacctgcc tcgcacctgg caatagagga cattttccgc tccgatcttc
    67021 agcgtttccg gatctagatc ctccaccgta tcatcgcata gatagatgcg gaagttattt
    67081 acggccccgt ttccatcccg ttgcgggtaa acggccttaa tgaaataggc cgtgtcttcc
    67141 gcttccacat aacaacggtc attgggcaat tcgatgacat acctgcccgt ggcgtcccgg
    67201 tggagatgtt ggtaaaaaaa attaacgaat tcccgccgga acatctcggc gccttgataa
    67261 taccatacac cttccttgtc gatcctgatc tggcaagggg ggctttttgc cacttcgttc
    67321 aaaattgtcc tcgcgtttca aatagtattt ctactgtcca gcaatattgt gacggggccg
    67381 tcattgacta agtcgatctt catcatggcc tggaattccc cggttccgat ttcccttacc
    67441 cggtccgccg ccgcggcaac gaaataatcg taaagatctt tggccttttc cggttcctcg
    67501 gaacggtaaa aggatggcct tcttcccttg cggcaatcac cgagcagcgt aaactgtgaa
    67561 attatcatca attcaccccc gatgtcggtg agggaaagat tcatcttgcc gtctatgtct
    67621 tcgaaaattc gtaagttgat gattttatcg accagataat cggcgtcctt gtctgcgtct
    67681 cctttttcga ccccgaggaa ggttatcagt ccccttcccg tccgggagat ttcccttccc
    67741 ccgacggcga cggcggccct ttccaccctc tggacaactg ccctcatctc tatcccgtcc
    67801 cgatctttaa tggcggggga tgttaacagc aatcagtccc gtcttcaagc attttattcc
    67861 ggaacaaaaa gagagaacag cccggctata gcgatgacat tggagaaagg tcatatgcct
    67921 agaaagaccc attgcctcca ggtatctttt ctgctatgcc gcctttccat gaaaccctat
    67981 gtactgctgg taaatccctg gatttacgac ttcgccgcat atgattactg gatgaagccg
    68041 ttgggattga tgtatatggc gtcctacctc aggaaaaacg gtttggatgt ccgattggtc
    68101 gattgcctgg acggttatgc tccgcaagcg gcatcaatgg cccttaggtc acgccgccct
    68161 ccccggcgga aaccgggggg ccacggcaaa ttcctccggc agcggattgc cgcaccgccg
    68221 ccgctgggca tgattgcccg gccttatcat cgttacggaa ttgatccaga ggtattttac
    68281 gccatggtca gatcctctgg gaagccccgg gcaatcatgg taacctccat gatgacctac
    68341 tggtatcccg gcgtcattgc ggccgtccgc attctcaagg atatctttcc ggaaagcccg
    68401 gtgatcctgg gagggatcta tgccaccctc tgtccggagc atgcccgggc tcacaccgga
    68461 gccgattacg tcctgaccgg ggctggtgaa gggcacatgg atttaattct cgagactatc
    68521 ctggggattt ctgcgtccta tcgaccggat acggataatt tggatgccct tccctatccg
    68581 gcctgggatc ttcttccgca tgtcgatcaa cttcccgtcc tcacctcccg aggctgcccc
    68641 tgccgctgtc catactgtgc cacgaggata ctcaacagga agttccggcg ccggagacct
    68701 tggctggtag ttgacgaaat cgcccattgg cggaaaactt tgggggttaa gaacttttcg
    68761 ttttatgacg atgccctcct cgtgcatccg gaagaaatgg ccatccccct gttgaaggat
    68821 atcattatcc gaaatctgga tgccggtttt cactgtccca acggattgca tctacgtgag
    68881 ataacaccag atattgctac gcttctcttc agggccggtt tccgaaccct ccgattcgga
    68941 ctggaaacga cggatagaga tagacaacgg gccctggggg ggaaggtgga gaatgtccat
    69001 ttcgaacagg cgacagcgta tttgcgggaa gcgggttata aatccgacga tatcgccgtt
    69061 tatctccttt gcggcctccc cggccagtcc gccggggaaa tcagggagag tatccgttat
    69121 gtccagtctc acaacgcccg gccgattctg gcggaatatt cccccctccc cggtacggag
    69181 ctgtgggatg ccgcctgcca gtcctccccc tatccgcttg ccgaagaacc cctgttccac
    69241 aacaattccc tcctcccatg cgcccatccc actctgacca ccccggagta tcacgaacta
    69301 aagcgccgaa gccgcggcct ttagctatca tccatacctt tttccaaacg ggaaaaggga
    69361 gatattttct tacaccttga ttatctgcac cgaaaaggtt aagatcaccc cccatgaaaa
    69421 aagaccaaat catcggcaca tggctttggt ggtgcaagct cctggccatc ggcggcatat
    69481 cgctcctgat ccttctctgg gggataagcg ccctctacaa cgcctatttg ctgaagaacc
    69541 cgcaggaatt cgtcatgatc ttcttttctg cctccttcat gattctcttg gggatcaccg
    69601 ggctgatcta tcccgcctat cgcatccatg cctattttaa aaatgattcc tttgaaaaat
    69661 ttccggaagt ataaagcgaa acagctcggc ctcgtcatct tcttccatgt tttcatagcg
    69721 cttatttatt tagcttccgc ctccggtgcg gatcttgatg acgcactgat ggagcgggtg
    69781 aaatcgttca ccttgaaaaa cgggattcgg gtcctgatcc tggagcgtca tttcagtccc
    69841 acggcctcct tctatatccg ctttcgggcg ggagcggcgg aggagcagga aggtaaaacc
    69901 gggctggcgc atcttttgga gcatatgatg ttcaagggga caaagaccat cggggggatc
    69961 agctcggtcc gggaagagaa actgctccgg gagataggca agaccggcga aacgctcgaa
    70021 cgggaaagga ccaaaggcga acggaaagac ggacaaagga tcaaagccct ggagaaaaag
    70081 ctggcggcgc tgcaaaaggc acaccgacgc tggtaccgtt ccaacgaaat cgaccgcctc
    70141 tatgccgagg ctggagccat caacatcaac gcctccacca gtcaggacat gattacctat
    70201 catgtcagcc tgccagcaaa caaggctgag ctgtgggcgc ggatcgaggc ggatcgcctc
    70261 gccaactatg tcttgcggga attctatcag gagcgggaag tagtgaagga agaacggcgg
    70321 cagcgcatag acgccgaccc cgacgggttg ttgttcgaac ggttcgcggc aacggcattc
    70381 atggttcatc cctaccggcg tcctgtcatt ggctggcctg atgatattgc ccggctcgac
    70441 atggaggatg tggactcctt tcaccgcctg gccaaggtgc cgcgcaacat ggtgattgcc
    70501 attgtgggag atatacggat taacgagatg cgccggttgc tcgaaaagta tttcggtgtc
    70561 cttcccgggc aaccggcggt gtttacaccg gttgcgacgg agccacctcc ccggggggag
    70621 aagcggatca cagtccattt cgatgccggt gcgcgaatga ttatgggctt cctcaagtca
    70681 ccgccgccga accgggagga ttatgtattc gatgttatcg aggccctcct ctcccgggac
    70741 cgttcttccc gattttaccg gcagcttctg gaaacgcagg ggctggcgga aagcgtccag
    70801 gcggtaaacg gcctgcccgg gaatcgttac ccgaatcttt tctgtgtttt cgcctcggtg
    70861 cgccatcccc ataagatcga tgaactggcc gcagcggtgg agaagatcat cgacgacctg
    70921 gcagttgaac cggtaccggc ggcggaactg gaaaaggtta aaaaccagtt gcaggccgat
    70981 ttcatccgcc gtcaggattc cgatgaagga ctcgccagcc tgctttccta ttatgaagcc
    71041 ctcctgggtg attatcgtta tctgagcagt tacggcaacc aaatctccac tgtcactgcc
    71101 gacgagatca tggcggcggc caggaagtat ttgaaccggg acaaccggac cgtggcgatc
    71161 atgacgaagg attgaagaaa ataatgttat tgctgataaa tatgtcctgt aaacggaaca
    71221 gtattaattt ttacgggtgc ctggcgggta tggcccaggg acggcggcgg caaggcggca
    71281 acttcatccg gaccgtcctg tctgtgctat tccttgccgc aatgatctta acggctgaaa
    71341 ccccagtcct tgccggcctg cccctgcccg atcccaaccg tctttcctat ccgccccttc
    71401 ggttttcacc cccggtcccc cagcatttca cgatagaaaa cggtctggag gtgtatttcc
    71461 ttgctaaccg ggaactgccg gtggtgaaag cgaccttgct ggtcaaatcc ggggccatgt
    71521 acgatgaccg gggaaaggaa ggaaccgcgg agctgacggc gactttgatg cggacggggg
    71581 gtacgaaaca aattccgccg cggattctgg atgacaccct ggcctccctg gcggcgacca
    71641 tcgagccctc cgccggtatg gaacaaatac agtggtcgtt tttttccttg cgaaaggatt
    71701 tctcccgagc gtgggatatc ttccaccaga tcctcagggc accccggttc gatccggaac
    71761 ggctggcgct cctcaaggag ttgaaaagcg aagaaatacg aaggttgaac gacgacccgc
    71821 aacaatgggc cttaaaggag tttttccgcc acctgtatca gggaaatcct cggggaagat
    71881 tgcctacggt atcgaccctg aatggtattt cacgatacga aatagagaag tgccatcgac
    71941 tccactacac cccggacaat atgtttctgg ccatttccgg cgatattgac cgggaagaag
    72001 tggaacgcat tgtccgggag acatttggct cctggccggc caaaagcggt cggatagcag
    72061 tgccgccccc tctcgccgcc ttccagacgg gaaaatatta tctaatcaag gatactcccc
    72121 aaacagtaac cgtcatcggc cgtccagcgc cgacaaagaa gtcgccggat tttttcgcct
    72181 tcgatgtggt ggaccatatc ctgggcagcg gtggttttcg ttcccgtatc ttccagcagg
    72241 tgcgcaccga tcgaggtttg gcttacgcca ccggaagttt ctaccgggcc cgggaggatt
    72301 atggcatttt cggggcctat gccctaacca aaacctcggc tgccggggag gctttgactg
    72361 taatgcagga cctcgtccgg accgtccgcc tgagcggtat tactgctgaa gagacggcca
    72421 gggcaaagag ttccatcctc aacagcttca tcttctcttt tgccactccg caccagttgg
    72481 tacaccagca ggcatcgctg gctttcgaag ggcttccgga gaattttctc tggcaatacc
    72541 ggaaacgtat cgactgtttg acctcgacgg aaatcaacca tgtcgcctcc aagtggctcg
    72601 attttgacca ggccctgatc tttatgctgg gcaaaagggc ggggtacgaa gagataaaaa
    72661 aaatacatcc agatttcaaa attataaagg tgagttatga ttgatgaaaa agcgttcctc
    72721 cgggtggcaa gccgcatcga tgcctaccgg caggaaatga tcgacctcca gattgccctg
    72781 accgccgttc cggccattgc cccggaaaac ggcggcgacg gtgaaaccac caaggcagag
    72841 ttgcttctcg aatacctgtc ccatcggcat attggagaaa tcgaggtggt taatgccccg
    72901 gacacccgcg tttcatcggg aggacgaccc aatgtcatcc tccggcgggc cggcggcgat
    72961 gcaactcggc atacctggat catcagccat ctcgatatag tgcccccagg ggaagcgggt
    73021 ctatgggatg cggaccctta tcaagcctat gtgcgaaacg gccgtctcta cggaagggga
    73081 acggtggaca accagcagga tctggtcgct tctctcttcg cctttcgggc gtgttgcgaa
    73141 gaagcagtgc cgacctatcg cgcccccggc ttgatcttcg tcgccgatga ggaaacagcc
    73201 agccattacg gtctggattt ccttttgcag catccccgta atcccttcca aaagacggac
    73261 ctgatctgtg ttcccgactc gggcaatgaa gcgggtgatt tgattgaggt tgccgagaag
    73321 agtatcctct gggttcgctt tcgtactttg ggcaagcagt gccacggcag caagccctac
    73381 ctggggaaaa acgcctttct agccgcatcc catttggcgg tccgtttgca cgccgctctg
    73441 ccccagcgct ttcccgcggt tgatcccctt ttcgaccccc cggaaagcac cttcgaaccc
    73501 actcgcagag atgccaacgt accgaatgta aatacaattc ccggtgaaga caacttttac
    73561 atggattgcc gtatccttcc aggctatgaa ctacagacgg tcaaagaggc catgaccgaa
    73621 accgcccgga ccatcgaaag ggaactgggg gtgacgatcg agatccttcc cgtccaggag
    73681 gtacaggcac cgccggctac ggatgccagt gcccccatcg tcatcgccct gcaggaagcg
    73741 atagcgaggg tttatggagt gcaggccaga ccgcagggga tcggcgccgg cacggtagcg
    73801 gcctacctgc gccggaagaa ttatccggcg gcggtctggt gccggacgac cccaatggcc
    73861 caccagccca acgagttctg cctcatcgac gacatggtag gcaatgccaa ggtgtttgca
    73921 catctttttc tacaaagatg aaccgtctct aggacaagga aaaaataagg tgacggcggc
    73981 atgaatcgct ctgaaaggag aaagttaata tccataaaag gttgcgtgga tatatttgac
    74041 tcggaagcct atctctgaca taatgccgat acgtatttaa gaaagcgtcc gccacatggc
    74101 cgtcggtcac tggcggctga tttgataaat atatgaccaa aattttgcag aacatacttt
    74161 gaaagccagt attcaggacc tgcataccga aacgcttcag tttcccgaca ccgttcagct
    74221 tcgcaacctg ttgggtgagc aggacaaaca tctccgccag ttggaaaagc tggcgccggt
    74281 aagaatcagc gcccggggca atgacctgtc cctgtccggt gatgtgtcat ccgttcagtt
    74341 aatcagggat ctgcttatcc agttgtatgg gatcatagaa aagggatatc cccttttttc
    74401 ccaggatata aactacgccc accgcattct ggcccaggac ggcaaggcca gtatcgaaaa
    74461 aatctttctc gacaaggtat tcgtttccgc caagaaaaga gtcatcgccc cgaaaagtat
    74521 ggcccagaag ctctacatcg atgccatccg gaaatacgat atggtattcg gaatcggccc
    74581 cgccggtacc ggcaagacct acctggccat ggccatggct gtttccgccc tgatggagaa
    74641 gaaggtgacc cgcatcgccc tggcccgtcc cgccatagaa gcgggggaaa aattgggctt
    74701 cctccccgga gacttggcgg aaaaggtaaa cccttatctg cgacccctct atgacgccct
    74761 cttcgacatg atggatatgg ataaggccac ggcgttgctc cacaagggga tcatcgaagt
    74821 ggctcccctg gccttcatgc ggggacggac actgaacgac gccttcgtaa tcctcgacga
    74881 ggcccagaat accacatcgg aacaaatgaa gatgttcctc acccgcctgg gtttcggctc
    74941 caaggcggtg atcaccggcg acgtgactca gatcgacctt ccccaggaaa agacctccgg
    75001 tctcattgag gcggaaaaaa ttctgcaaca ttcagaaaga atatcattta ttcatttttc
    75061 cgaactcgat gtggtccggc atcccctggt acaggagatc atccgcgctt acaatcacct
    75121 cgatcagatg cgcagcaagg cccattagga atatcccacc atgatcacgt ggaaagaaat
    75181 agccgccaag attgttgtcg aaatccagaa gccattcgct catcccgatt tgatcccaat
    75241 gttttttaag gatcccgcca agcagcgctg gcttatcctc atcctgctga gcattgtcct
    75301 caccgttatg ctgctgccta cggttaccat catggcaccc cattataaga tcggagccat
    75361 cgcagataaa aacatcaaag cggaccggga ctttctggtc gaagaacgga cagcgacgga
    75421 attgaaacgg ctggaagcgg cgaaatcctc ccggaacatc ttcgatttag acgatacggt
    75481 gctgccgcaa atccggggca acatcatgaa agccttcctt gcggttcagg aggcggtcaa
    75541 gaaaacagcc gccgggacaa cggaagcatc acgcaccgcc tctgtccaga agcaaaagat
    75601 cctggtcgtt atggaacagt acctgggggt gcgcctttcc gatcaggatt actcggcact
    75661 ttccagtcat cgtttttcat cggaaatcgc ggatcgcatc gccgagataa tcaccaaggt
    75721 ctatcaagac aagatgatca ccaacgtcga tttttcacca ctggaatacg aacgggggct
    75781 gatagtgaag aatgtccgca cccgtatgga aagggaggaa aaaaacgccg acgaaatcct
    75841 ccagatgcaa gatgctaaat ggattttaag acaaaaggcc ctggcgacca tggcggaaga
    75901 cgatgccgtc ctgaaaaaaa cagtgatcaa cattgccgaa aagctcctgc aaccgaacct
    75961 tacctacaat cgcctggagt cggaacagcg tcggcagtcg gcggtagagg ctgtaaaacc
    76021 ggtgtatttc gaggtacaga agaacgaaat gatcgtccgg gagggagaga agattagcgt
    76081 cgccgatctg gacaaactca acgccttctt tcgggagcag cgccaacaga agacttcccg
    76141 ttatgccgga tttatgggga tgtttttact gatattttcc ttgacgacgg cattgctgtt
    76201 cctctcacgg aaggccttcg gatcccagga tattcaaccg ggtcccaacc cccggctgtt
    76261 gtgcctcacc attatcatca taatccaggt cgcgctcatc aaggcggtgc tgttcatgtc
    76321 cgacgccgtt caccaggcca tgcccatcct ctcgaaagag gcgatcatat tttccatccc
    76381 cttcgccgtg ggcccgctat tgaccgctta tttgttcaat cgccgcatcg ccctgctcat
    76441 gtcgctgttc ataagcttcc tcattccctt tctcatcacc ttgcccgagg cctatctgtc
    76501 tctgttctcc tttctgggtt ttgtggttat catctgtcat aacgatcatt atcagcgacg
    76561 ttccgtattc atcaaaaccg gcctgctctt ggggataagc aacggtgcct ttattctcgc
    76621 cattgccctg ctgtctgctc atccgctctc tgtggatacc atttggaaaa tagtttccgg
    76681 cttcgccggc ggggtgatga gcggcatcat cgtggccggc atcagtccca tttttgaaac
    76741 cgccttcgac tttaccactg atatcagact gctggaactg gccaacctca accagccgat
    76801 ttttcaaagg atgataatgc aatccccagg cagctatcat catagcatca ttgtcggttc
    76861 catggtcgag gccgctgcgg agaccattgg agcggatcct atcctcagca aggtcagtgc
    76921 ctattaccac gacatcggca agatgaaaaa gcctgtttac ttcatcgaaa accaggaccg
    76981 gggtgagaac cgccacgaca agctttcccc gcggatgagc gcccttatca tcatcgccca
    77041 tgtaaaggac ggatgcgatc ttgccgacga ctataaactg ggaagcaaga tcaccgacat
    77101 catccgccag catcatggca caagcttggt gagctatttc tacgaaaagg ctaaaaggga
    77161 caaggcgcct tccatgagat cgcttccgga aagccatttc cgctatcacg gaccgcgtcc
    77221 acagactcgg gaagccgccc tggttctcct gggggatgtc gtcgaagcct cttctcggac
    77281 gctcgtagat ccgacacctg ctcggatccg aaatctggtg cgggatcgca tggataaggt
    77341 cttcatggat ggacagttag acgaatgttc tttgaccttg aaggatctaa atctcatcca
    77401 ggatagtttc gttaggactc tgaacggtat tttccatcat cgcatcagct actacgacga
    77461 tgttttggga aagaataaca agcaggtgat caaatccttt tatgaatatc cggatcgaaa
    77521 accggcagaa aaaatcaccc ataaatcgcc ggcagattcg cagcagcatg gcgagactgc
    77581 ttaggtatct cgggcatagt gatgcggaag tgagcatgct gctggtggat gacgagggga
    77641 taagggagat caaccgctcc tatcttcagc gcgattattc caccaatgtg atctccttcg
    77701 cgatgcggga aggtccctac ggtcagttgc acccccaggt gctgggggat atagtcattt
    77761 ccgtggaaac ggcctgccgt cagggggata agggcgcact ttcaatgaca gaatccctct
    77821 ctttcttcat gatccatggt ctcctccacc ttctgggcta cgatcatgaa ggagtggatg
    77881 cccgggatat ccagcgcatg gaggagaaag aagacgaatt gttcttcctc ctccacggct
    77941 tttccctgca gcgctgatcc atttggctat ccgttcatcg ccttggcgat gtgtttgacc
    78001 atctggccga agaccccgac gattatctcc ggggccagaa aataattggg atgtcgagtg
    78061 taaagctctt ctgtcagcaa ccgggacgca cggtcgatat cacccgtttc ccgatacacc
    78121 gtttcgatgc gggttcgaag tttccgggcg gccgcgatgg actgcagcac ataatcgcgg
    78181 gcctcgggac ccacaatgta gccgtgatgg tccgctccga aatattccac ttcgaggtcc
    78241 ttcaaccgct ccaaactttc ctgatacagg gtgtaattgg agtttgccga ggtaaaaatc
    78301 gcgtcatcga tgggaattcc tccggcatcg gaaggaagaa gggtcttcag ttccgggatg
    78361 taggcggaaa tagaacatga ggagtgacct ggagtttcca tgatccttac ttccagatcg
    78421 cccactttta aaaggtcgcc gtcccggacg atggttccct gaacatcgtc tcgccaatcc
    78481 agatcgtggc gggcgagaac atcaaggcag cccatctttt ccgccaccat gcggctgaag
    78541 gcgttcatgg tttccactgc cttcggcatg gtgagaatat cccaggcccg ctgggaggcg
    78601 taaaccttca gcgccggata tttcctctgg aaatatggta cggtgccaac gtgatcgaaa
    78661 tgtgaatgga ggatcagcaa tgaagtgatc ttctcccctt cgatgccgaa ggccgccatc
    78721 tgagctagaa catcctccag gatacaactg agcccggcgc tgatgagcat cgcttcccca
    78781 ttcacagaca aaagatacat gcccgattcc atgcggccca aataccagag tctttcgttt
    78841 accttgcttg cgattctaat tctcataagt cccccttgct atgaaatatc cccgacaaca
    78901 atatgcgcct tctcctttag ttgtcccaca atttgattga aggcgtcata tttctttttc
    78961 tcctttagaa agccggcaat cctgagcttc gtatcctgat caagttgctg ggtcctggcc
    79021 tgatgatgtt ccagaacctg gatgatatgg aacccatatt ctgtttccac aatggggccg
    79081 atttcgttct ttcttcggga aaaggcggcg tcttcgaagg gcttcaccat ctgtcccctg
    79141 atgaacgtcc ccagatcacc tccctgggcc ttgctggggc aatcggaatt tgtcttggcc
    79201 aactctccaa aatcttttcc ttccaagatc tgttgacgaa gtttttccgc ctttcccttc
    79261 cggatagccc gagcctcatc attctcccca tccttcttgg ctaccaagat atgccgtaca
    79321 tggacagcct cggggatctt gaatttatcc tggttgcgtc ggtagaattc ctggatctcc
    79381 ttctccgtcg ggggagattt gactcgtttt tgactttcga cgagtttatt tattttaatc
    79441 cccagggcga tttccttccg gagttgctcc ctgtttatgc cgtttttttt cattaaatca
    79501 tctaaactgg cgccggaggg aagacccgcc ttcatttcgg cgagtgctgc atcgatctct
    79561 ccttccgttg cggtgatttt gagtcgggtc acttccttga gcatcaacgt ttgcaacacg
    79621 aaatcatcaa taatctgctt ccgcatcata ccgcgcatcc ggtctgcccg ctccggagat
    79681 agggaaggag aaagagcggc cattcttttt tttacatccg catccagttg cgcccgggtc
    79741 atcttcaccc cgtgcacttc ggcgacaatg tcggtcggta ggcggtttac cgccaccggt
    79801 gatgtttgtg gattgggagt cggcgcaggt ttgaattcgg cggcaggggc tgattcattc
    79861 attccctgga tcgtccgagg ctcgtcggta cgattacaac cggttacaac agccaggaat
    79921 gcggcagcga tcaatgccca aatgactttt ctcaattaat atccctcatt ctttcggtgt
    79981 ttggtcggta atatatgcac aatcaggatg cgccgtcaag gaagacccgt ccccttttac
    80041 accgatgacg acatatatcc ttttttataa aaaggtccaa aaatagccgg atatttacat
    80101 ctttcgccgt gcccgttaca ggcatgcagg tttatgcgtc tatgcgcgca aggaggtaat
    80161 attaactgct tgcaatcccg tttatctcca tgttacccct ctggctatga agaaaaaggt
    80221 tttagatctc ggtgtcggtc ccattctccc cctcctgatg aagatgagct ggccctccat
    80281 gctggccatg ctttccatgg ccatatacaa cgtcatggat tccctctggc tggcccgatt
    80341 gagtccccgg accatcgccg cctttaccat tacgttcccc ctgcagatgg tctttgccgc
    80401 catcggggtg ggtatcgggg ttggggctgg atcgtacgcc tcccgcatgt tcggcgccgg
    80461 cgaagcggat aaggcaagaa gaaccgccgg acaggtcatc tttttatccg tcattgccgg
    80521 tctctcactc atcgcggtca cctatgcctt cccagatttt ctcctccgcc ttttcggcgc
    80581 ttccggggaa acgctcgttt tggccaaacg ttatctgatg tgggtcgtcc tgagttctcc
    80641 attcctgctg ttcatgatga tggccaacaa tcttttccgt gccgaaggtc tgcccaatct
    80701 atccatgtat gccattctaa cctttacctt cctcggggcg ctcctggatc ccctgctgat
    80761 cttcggctgg ggaggattcc cggcgctggg tatcgaaggc gcggccctgg cggccatcgt
    80821 cggccaggta gcatccggtc tcctttcgct atatttcctc ctttacagat cgttgcggta
    80881 ccgaattgac ttacaggatc tccttcccga tccgaccata attcgggcca tccatcagac
    80941 aggattgccg tcggtgctga tgaatcttgt cttcagcgcc gtcatcatca tttacaacaa
    81001 cattcttgcc tattacggtc atctagcact ggcgacactg gggatttgtt tccgcatcaa
    81061 cggcctggtg atgatggttc tcttcggcat cggccacggg gtcatgccga tggtgggcta
    81121 caatctgggc gccaagctct atgaccgcct catcgccacc gtgcaggtcg cggtacgttg
    81181 ttccatgctt tttgccgggg ccagcagtgc tatcatcttt atcggcgccg atttaattct
    81241 tagttctttt accgatgatc gggagctgct ggtcatagcc gctccagccc tgaagatctt
    81301 catctccact ctcgttctgg ccgcaccgag catcgtttgg atcaatctct ttatcggtct
    81361 cggcaaaggc ttgaccgcca tgacgctaat cgttatccgg gatgccctgt ttcttatccc
    81421 gctaatgtat ctcttttcct tgtggctggg tcgcgacggg gtatggatgg cccaacctgc
    81481 atccacggcg gtggccttcc tgatcatctg ggtatggtcc ggaaaggaat tggcggcaat
    81541 ccggaatctg aaggagcaga caaatccctg accgaaaaga tagaactgta ttgcgcgccg
    81601 agaagacatt gtttttactg atcatgagac gtaaacagaa tatttttcca aaagaagtta
    81661 gaacgactga tattgcgaga tggtggaaaa tgcttattat tttaaaaaaa tgcccctgcg
    81721 acgggcgacg gcacaggggc atgacgcaaa aaaatagtga acagaaatca gctctagaga
    81781 accgaagcca cgactatagg gtttgaacgt ttacagccgc cggtcctttc ttcccctgtt
    81841 cgatatcgaa acttacccgt tgtgactcaa acagcgtttt gaatccagaa ccctgaatgg
    81901 cactgaaatg gacgaagacg tcaccgcctt catcattctc gatgaaaccc catccctttt
    81961 tttcattgaa ccattttact ttaccctctg ccaaaacata ccctcctttc ctccatataa
    82021 aaaataaaaa accactccgg ataattcatt atacccgagg tggccataaa aatacttctg
    82081 aacatttttt ctcaaaatcg ggttgcaatc ccgttattat ctccggcgca ggccattgat
    82141 tgataagccc tgtttcttga aatttcccgc tatcactact tgtcgaggaa agcaagtctt
    82201 ttatggaaca ttttatgctc atcaaacttc ccttttcgaa cgatgacagg cagagagatg
    82261 cccaccggaa accacggcaa gctcgggggc agtttcccag cagaccggca acgcctcagg
    82321 acagcgggaa caaaacaagc aaccagtttc aattgtttcc acttccccga gagaaacaac
    82381 tatctccctt tctcttttca cagtttgacc ggaaaaactg gggacggcat tcatgagtat
    82441 ccttgtgtag ggatggcggg gattgccgaa gatcccctca ttggggccga tctccacaat
    82501 gccaccgccg tacatcaccg ccaccctgtc gctcatgaac cggatcaccc ccatatcgtg
    82561 ggaaatgaaa agataactca ggttgaatct atcctgcaga tccttcagca aattcaggat
    82621 ctgggcctgg accgatacat caagagcgga caccggttcg tcggcgatga caatctcggg
    82681 attcagggcg atggcccggg caattcccaa ccgctgtcgc tgaccgccgc tgaattcatg
    82741 gggatatctg ttcttttgtt cggacgataa ccccaccatt ttcaacagac cgtccagatc
    82801 gcccagggac acaccgtcga tcctactttt gtggatccgg aacggctccc cgacaatgtc
    82861 accggcgctc attcggggat ttagagaggc gtagggatcc tgaaatatat actggacctt
    82921 ttgcctatat tcgtgaagtt ccgccttctt tgcgacgaag atgtcgattc cccggtaata
    82981 aatctttcct gaagacggcc tctcgataca tgcaaccatc ctcgccagtg ttgttttgcc
    83041 gcagccgctt tctcccacga gtccgagggt ttctcccgcg tgaatctcga agctgacctc
    83101 ccgcacggcg tgacaagcat ccggggcaga agaaaagagt tttctcttgg gtgcaaaaga
    83161 cttgtctatc cgttgcaccg ataagagggg cgcatccatc atgtcagtgg tatttccagc
    83221 agcgcacata ttggtgtgga tgatcatttt ttcgccaggg aggttcgaga ttccggcaca
    83281 caggcatgac atccacgcac cgttcgtgaa aacggcaacc ggaggatcgc atggcggcaa
    83341 gatcgggtgc gccgccggaa atcatgggca gacgcttccc cggtccgaat ttgccgccga
    83401 tgcagggtat cgattccagc agccctacgg tgtaaggatg cagcggccgg gaaaaaatga
    83461 cccctgcttc agcgtactcc acgaattggc cggcgtacat catggccacc tggcgggctg
    83521 tctgggagat gacgcccaga tcgtgggtaa tgaggatcac cgctgtcccc aattcgcgtt
    83581 gaagttgttt catgagttcc aagatctgtg cttgaatcgt tacatcaaga gcggttgtgg
    83641 gctcatcggc gatcataagg cggggtcggc aggacaacgc catggcgatc atcacccgct
    83701 ggcgcatccc gccgctcaac tgatgaggat agtctctcat ccgttgttcc ggtttagtta
    83761 tacccaccgt tcgcagcatc tccaccgccc gctcccatac ctcccgccgt gtgccaccct
    83821 ggtgcaaccc caatgcctcc cggatctggt cgccgatacg gaagacaggg ttcagggagg
    83881 tcatgggttc ctgaaagatc atcgaaatcg atttcccccg gattttcctc atctcttttt
    83941 ccggcaggcg gaggagatca tggttgccta aaagtatctt gccgccaacg tattttcccg
    84001 gtggtgtcgg caccagcttc attatcgaca gggaaaggac cgtcttgcca catcccgact
    84061 cccccacaat ccccagggta tccccgacct cgacctggag gctgacgtcg tcaacggcgc
    84121 ggattacacc ccgccgtgta tcgaagaatg tggaaagacc ttctactttg agcaccggcg
    84181 ccatgcctgc tgcgcctctc tattttcggc aacggtttct caggaaccgg acgaggaggc
    84241 gaaccccgac gcctgaagcc cctttgggtt gataggccgt ttccttggtc agccaggctg
    84301 gaccggcgat gtcaagatgg acccagggat agggacccac gaacttgctc agaaaagcgg
    84361 ccgccgtgat ggcgccaccc gtccgtccgg cggaattttt gaaatcggcc acctcgctct
    84421 tgatctgttc gtggtagttt tcccagaggg gaagctccca gaccatctcc ccggtctgct
    84481 gtgaggcacg ctttatctct tccttcaatg gtccgtctgc ccccatcatc cccgtcactt
    84541 gctcgcccag cgccacaatg caagcccccg tcagggtggc gacatcgatg atggccgcag
    84601 gttcgaatct tgaagcgtaa tgcaaggcgt cggctaaaat taaccttccc tccgcatcgg
    84661 tatttatgac ttcgatggtc tttccggaca tggatctgag aatgtccccg ggccggcagg
    84721 ccttgccacc aggcatattt tccgttgccg ggatcagggc cacgacattg atgggaagtt
    84781 caaggtctgc ggcggcgttt acagcgccca gtacggcggc ccctccggcc atatccgact
    84841 tcatttcgtc catcttctcc gctggcttca aagagatgcc gccgctgtca aaggtaaggg
    84901 ccttgccgac aaaaacgacc ggagatgttg cctcatctcc gcccagatag gaaacgatga
    84961 taaatttcgg ctcttgatcg ctcccgctgg ccacccccaa cagagagttc atccccaggc
    85021 gcttcatttc cggtaccgaa aggatcgtta ccgtcaattt ggcccttcct tccgccagga
    85081 tctgcgcctt ttcggccaag acggcaggtg tgagttcgtt ggcgggcatg gatgtgagat
    85141 cccgggcgag ataagccgct cgacagatct ttacggccac ggcaacctct gtccggaccg
    85201 ctttcaatct gctgcgttcc ggcactaaga tagttaaaaa ctgcaggtct tgcccttcct
    85261 cccggtccag tgttttgaag gcggaaaacc tgtaaaggcc gagagtcact ccttccactg
    85321 cggcccccgc cgcttccggt ccttcgatcc gcagataaga gggatccaga agcaaaatca
    85381 gcttctcctc ccgatgtgat tgtgccgctt ttccggcctt ggcggcagcg ccgcggagct
    85441 tatccagggt aaattcatcc ctttttccca gaccgatcaa gattatcatt ggcggcgccg
    85501 gcccgtgttc aggaaagaaa accgtctccc gatagagttt accagccagg tcgtttcttt
    85561 tgaggatctt ccggatcaga tcgcgggtct cctgtccaac gggccagtta tccgccgctt
    85621 cgaacctttc cccctgaaag acaaaaattc ccagacatcc ccaatcctga tccgtgccag
    85681 cgacggcgtt cctgacggtc acttttaact ctgacttcaa tatttcttcc tcccgtcccg
    85741 gtgggaaaat aaaaaagcgg ttgattaccg gggacaagaa atcaaccgct cgctcagttt
    85801 tgcttcacaa tctgactaaa atgatcctac ggaagacgac gcagccgcgc atatatgcaa
    85861 agcgtatcat cggcatccgc tgtccatgga gatcatattt ggaggcggca accggattcg
    85921 aaccggtgaa taacggtttt gcagaccgct gccttagcca cttggctatg ccgcctgatt
    85981 ttctggagcg ggcgaacgga tttgaaccgt cgacgtccac cttggcaagg tggcactcta
    86041 ccgctgagtt acgcccgcaa gtattgatca catctggggg ctgactataa catcagcctt
    86101 tttttgtcaa taaaaaagtt caggaagtct cgttgtcacg gctctcttcg gcgcggtcca
    86161 cgagccagag cactgccgcc atatattcag tcattgcagc aaagaagatg ttgttgtggt
    86221 ttgcacccgg tacccggagc atccttttga ttggggccgg agatgcttcg tataatgcct
    86281 ctccctcatt aaaagcgatt atttggtcga attgggcgtg aatgataagg gttggcttag
    86341 atatattctt gattttactg atattccttg gcccttggac ttcgtcaaga tgaaagtact
    86401 ccatttcgac gcccatcaat ctcaataatg gaccaaggta ggcaaaaccg ctctcgatga
    86461 tcaatccgtc catatcatgt tgacggcggt gggcgatctc cagagcggag gcgctgccga
    86521 gggatctccc cataacgatc atgaggccag taaaaccctt ctcccggagc catcctttta
    86581 catggtcgaa tatcaccaag caatccgtca tcatggcgct taccgtgggg atgccggatg
    86641 acacgccgta gccccgataa tcagcgatga gaaaattgat cccccggtcg gtgtagaaag
    86701 gggctatatc ctgataatca gatgcaattt caccatttcc atgaaaaaag ataatattgg
    86761 cggcggccgg agattcgagg tatgcctgga cttgaacata aaccccatcg tctaccggaa
    86821 ccatcagtga tgttgcctca cggtggttca tggtcaggga ccagtcgggg cggggataga
    86881 agagaactcg taatatctcc ggccggtcga aagtccggta atcgcttatt tcagccatcg
    86941 gtcccctctt ttcccaacag gatgtgccgg taaaatttcg tcaggtaatc caagccggaa
    87001 tagacactga gaatcaaggc gataaacaga atggtggtac cgactacatg ggcgtccgca
    87061 cctaaaaatg gataatgaat cataagagcc gatacggcga agacctgaca taaagtcttc
    87121 tgcttgccta gccaactggc ctggatgata tatccctccg atgaggcgat gtttctgatc
    87181 ccgtccacgg caaaatcccg gatgacgatg atggccacca cccaggccgg gatcctgccg
    87241 atggggatca tcaggatcat ggcggtgttg attaccagtt tgtcggcgat gggatctaaa
    87301 aattttccca gcgtagtgac aatccggtag cgacgggcga catacccatc aagcatatcg
    87361 gtaaaggaaa cggcaatgaa cagggcggcg attatgagac tcatcgtctg gccgggggac
    87421 aagaggagaa gaaacagcac cgggacaaac gagatccgta ccatcgtgat ggtgttgggc
    87481 agattgaaga tcttcttcct ctccggcgaa aggggcaggg aatcgaaata cctttgcaga
    87541 tggtctatca taaaatgatg attatctttt tttcacggcc ggatggaaac cccacctgtt
    87601 caatcgctgg ctttgcggtt gttgcgatca caagcatcat ttcttcggga ctcgctgcca
    87661 gtcggataga aaggcttcca ctccccggtc tgtcagggga tgctgggcca actgcttaat
    87721 taccttgaag gggatggtag cgatatccgc acccatcatg gccgcctcca ggacatgtcc
    87781 gggatgccgg acactggcga cgataacttc tgtttcatac ccatattgat cgtaaatatt
    87841 gattatctgc tccaccaggg ccatgccgtc atgggagatg tcatccagac gccctacaaa
    87901 cggacttgcg taggccgccc cggctttggc ggccatcagg gcctggaggg gagaaaatac
    87961 aagagtctga ttcaccggga tgccctcggc ggcgaagaga cgggtggcct tcagaccgtc
    88021 cagggtcatc ggcactttga taacgacctg gtctcccagc ttggccagtt ttttcccttc
    88081 cttgaacatc tcaccggcct ccagactaac gacttccaga cttaccggtc cgcttacctc
    88141 cttgagaatt gccctcacca cttccgcaaa ggttttcttt tctcttgcga tcaggctggg
    88201 attggtcgtg actccatcga ccatcccgaa actggccgct tctttgatct cttcgatatt
    88261 cgcggtatct atgaagaatt tcatgatccc tccttattct tttcctgctg ggaagcattt
    88321 tgatagtttt ccatgcaatt gcgactgcag aaataaagga tttttccgtt ttgccgccat
    88381 atgcaggcat tgctgatcgg gatataagtg tgacagtagg gatcctcgac aagttcctcg
    88441 ccagcggtga gattttcccg ctgttcttta gagatggggt tgacggtctt cttccccggc
    88501 aggaagagca tcctggtgag ccggtaaaca agatagaaaa cgatgatcgc gataatgagc
    88561 cggagaatca ttttttcttt ccgcaggata acttcttgtt attgactaat caactcgacc
    88621 gccgctcgat cgtcttcgtt tagacggatc agtaatccct gcacggagat gccgaaagca
    88681 ggatgaacca gataaccggc aagttcacac atggggatca ggacgaaacg ccgccggtgg
    88741 cattccggat gaggaattac caggtcatct tcccgcaaaa gttcctggcc gtaaaaaatt
    88801 atatccaggt cgataagccg gggaccccag cgctgggttt cctggcgtcc catcacctgc
    88861 tctgtggccc gaaggttttc ccacaggcgc cggggtgata gaatggtgcg gatctccacc
    88921 actgcgttga cgaaactatc ctgattgcgc actcctactg gctccgttcg gtaaaaggaa
    88981 gaggcccgca agagcttcac ccccgtcatg ccccgcaact tttccagtgc cagccggcac
    89041 tggaaaagcg gctcttgcag gttagagccg aggccaatat aggcgattgt gccggcagcg
    89101 gtcatcttag cgcgttttca gtcccagcac atcctgcatg tcgtagagcc ccttttgctg
    89161 gttcacgatc catttggccg ctcggagcgc cccccgggca aaattgtccc ggttgtgggc
    89221 gcgatggatg aactccaggc gctcgccgtt ggtgacgaac atcacattgt gttcgccgac
    89281 gatatcgccg ccccgaaggg tctggatgcc gatttccgcc ttcgtccgct cgccgatcat
    89341 acccttgcgg gcatagacgc catcccgatc gaggttcctg ccgagcttcg cggcgatgat
    89401 ctgggccatc ttcatggccg tgccgctggg ggcgtctttt tttagcttat gatgcgcctc
    89461 caggatctcc acgtcgtaat catcacccag gatagccgcc acgttttcca gtactttgaa
    89521 catgacgttt accccaacgc tcatattggg ggcgataaca cagggcgtct tcttggctaa
    89581 tttagcggcg gtcttcaact cattgggggt aaagcccgtg gtgccgatga ctattggctt
    89641 gccgaccttg gcggcgattt ccagatgccg cagggacgat tcatggtagg taaaatcgat
    89701 gaccacatcc ccctccttga ccaccttctt caagtcgtcg gcaacgagga ttcccgtcat
    89761 ggccatgccc aacacttctc ccagatcctt acccactgcc ggatggcctg ttgcctccac
    89821 ggccgcggat acttccatgt ccgttgccgc tgtgatgaga tgaccgatcc tgcctcccat
    89881 cctaccgccc gcacccatga gaatcgtctt gatcatcttt tccgctcctt atctcagcaa
    89941 cccgtagttg atcattatct tacggagttt ctcgtaattt gcctccgaca tcttagccag
    90001 gggtagccgc acttcgtatt cgattttgcc catcatcgct agggcggcct taactggggt
    90061 cgggttcgtt tcgatgaaga gagaatccac caggggcgcc atccgataat gaagattttt
    90121 ggctttctgt aaatccccgg cggcgaaggc gtccaccaag gccgccatat cttttgggac
    90181 gatattggag atgacggaga taatcccgtg cccccccaga caaagcagtg gatatgtgaa
    90241 gaaatcgtct cccgaaagga cggcgaaatc aggaccgcaa agctggatta cttcatgcat
    90301 ctgctttaag gatccggagg cctctttcgt gcccactatg ccgggaacct tggccagtct
    90361 ggccatggtt tccggcagca aattgacgcc ggtacggccg ggtatgttgt aaacgataac
    90421 aggaatcttc accttttcgg cgatcgtttt gtagtgctga tacaggccct cctgggtcgg
    90481 gcggttgtag tagggacaga ccatcaaggc gccgtcggcc cccacttccc gggcatgctc
    90541 cgtaaggcgc agcgcttccg ccgtggaatt agagcccgtg ccggcgatga cgggaacacg
    90601 cttcttgacg gcatccacgg ttatttcgat cacccggtcg tgctcttcat gggacagggt
    90661 cggagattcg cccgtcgttc cgcagggcac gattccgtcc gtcccatttt ctatttgaaa
    90721 ctcgataagt tcccgaagtt tttcttcgtc cacccgcccg tttttgaacg gggtgacaat
    90781 agcgacaatg gctcccttaa acataggccc tccttaaaat tgatttagcg ttgcttttta
    90841 acacatccgg tattttctca ccacgaataa gttcacgaag atcctcccgc ttccgaatca
    90901 cgtgataacg gtcgcccatg actagcactt ccgcggcccg agggcgagaa ttatagttcg
    90961 aagacatggt gaatccatag gccccggcgc tcatgaccgc catcaggtct ccccgggcga
    91021 agcccggcag ttttctttct ttggccagat aatcgccgga ttcgcagatc ggaccgacga
    91081 catcggcgat gatttcccga cgacgcgatt tccggacctg ttggatgttg tgatatgatt
    91141 tgtacaggct cggccggacc agatcgttca ttcccgcgtc gacgatgatg aatttcttct
    91201 cttcattttc tttggtataa aggacagcgg tgaccaagat gccggcattg ccgacgatta
    91261 cccggcccgg ctcgaaaatg aaggtacagg atatgtcccg tgaggcgcct attatggcgt
    91321 cggcatactc cgaaggatgc gggggcgtct cgtccttata ggtaattccc aatccgccgc
    91381 caagatcgag atagcggatg tcgatccccg ccgcacgcag gacctgaagg agtttcttca
    91441 ggcggtccaa ggcgtcgatg aaaggggaca tcttcgtcaa ttgggagccg atatggcaac
    91501 ttacgccctt gagatccaga tgacggtatt ttcttacctg aagataggcc tgatgtgatt
    91561 tttccacgtt gatcccgaat ttgttatcct tcaaaccggt ggatatgtaa ggatgggttt
    91621 ccggatcgac atccgggttc acccgcagcg ctacccccgc ctttttcttc agccggccag
    91681 cgcaagcgtt gattatctcc aactcctgaa aggattcgac attgaacatc aggatgccgg
    91741 tttccagggc atattcgatc tcctcgatcc gttttcccac cccggaatat acaaccttcc
    91801 ggggatccac tcccgcccgg agggcgcgat agagttcccc gccggagacg atgtccaccc
    91861 ctcccccttc ccggatgaag atcttgagga tagcgatatt ggaattggcc ttagcggcga
    91921 agcaggtgat gtgaggaatg gtgtcaaagg cgtcgtcaaa gacccggaag tgattcttca
    91981 aggtgcggtg gctgtaaatg taaacagggg tgcccaccgc cgccgcgatc ttggctacgg
    92041 gcgtttcttc acaccacatt tcccggtcct ggtaatgaaa atcatgcata ggtcaaggcg
    92101 tcctttagtt aattattacg gtttaaacgt tatctcgacg gggttggaga ttgcaatcac
    92161 ggaacctccg ggcgatcccc attcgaggcg gtaaccataa tgagtttcct ttgccaccga
    92221 gcggtccaga taacgatagc tctctgtccc accgtcctcg cccgcgaaat tcgctgccgg
    92281 ggcttttatg aggaggatat aatcctttgg gcaaccgggg cattcatcct tttcaaggtc
    92341 ccaactggct ctccatatct ggaaatattt gccctctgaa tccaactccg gcaccgattt
    92401 aatggtccac ttgaggagga cgccgtccac agccgccgca gctgtgagat cgcttatcct
    92461 ggccggcggc tgctgttgcc taggcacggg atcgcctttc tttccgcagc cggcaatgct
    92521 tccgatcata atcaggataa aaacgatcag cagtaccgcc gggacatcac tcgccctgtt
    92581 caatttcccg aatcctctcg gctacccgct ccggggaagt gccgccgaac gtctttttca
    92641 tattcacgga cctggccgcc ttgaggcggg gaaggacatc gtcggcaaac acggcgtgaa
    92701 attttttgag ttcctcgaag gtaatcatgg cgagttcctt cccggcgtcc agacaatagg
    92761 cgacgatccg gcccacgatg ctgtgggcct cccgaaaggg aacccctttt tcaacgagat
    92821 attccgccag gtccgtcgcc gtggtgaatc cgagctccgc ctctgcctcc atccgcggac
    92881 gaaggtaaac gatacgggtg atcatttccg tgaggatcgc caccgcggcg gtaacggtat
    92941 ctacagtatc gaacagcggt tccttgtcct cttgaagatc acggttatag gtcatgggta
    93001 ggcctttgag tatggtaagg atggcgatga gatgaccata aacccggccc gtttttccac
    93061 gaatcaattc cgccacatcg ggattcttct tctggggcat gatactgctc cccgtcgtga
    93121 aggcatctga tatctccaca taaccaaatt cctgggacga ccagatgatg aggtcttcac
    93181 aaaagcggct catatgcgtc atgagcagcg aggcggcaaa gatgaactcg gcgatgaaat
    93241 cacgatccga cacggcgtcc atgctgttgc ggctgatccg ggggaattgc aggagttcgg
    93301 ccgcatattc ccgatccagg gacaggcttg ttcccgccag ggctgccgat cccagcggca
    93361 tgacgtcgat ccgtttacgg cagtcccgga agcgctcctc gtcacggtcg aacatctccc
    93421 agaaggcgag gagataatgg gccagcaata ccggttgcgc cttctggaga tgggtataac
    93481 cgggcatcac gatttcgatt tccttcttgg ccgtttctac cagccgcttt ttcagatcac
    93541 ccacaagctt cagcaaggct tcggtttgtt cccgcaggta gaggcgcaca tcgagggcga
    93601 cttggtcgtt gcggctccga cccgtatgaa gtttttcccc cgcctttccg atcctggcgg
    93661 tcaaggcctt ctcaatggcc atgtggatgt cttcgtcact ctcgtcaaag cggaagcttc
    93721 cagattctat ttcctctccg atggcctgca aacctcggca tatctcctcc gcctccgctg
    93781 gggaaatgat cccctgccgg gccagcatcc gggcgtggac gacgctgccc gcaaggtcgt
    93841 aaggatagag gcgacggtca taggcgaggg atgaggtgaa agactccacc cgacgatccg
    93901 tatccttttt gaaacgtccg ccccagggtt ttttcgattt agacttcttt tctttcattt
    93961 ccatagagat tcttattgta tgcccataag caggtcaaga tcatttcctt cgctcgcctt
    94021 tgcggcgctc tttgcctttc cttgcgtcct gcccttccct ttgcatggct ttaatcttca
    94081 ggcgcagggc gttgaggcgg atgaagcccg tggcgtccat ctggttgtac acctcttcct
    94141 tttcaaaggt tgcgaaatct tccgagtaca gcgagaaggg tgatttcctg cccacgacga
    94201 tgacgtttcc cttgtagagt ttcagccggg ccgtcccggt aacgttttcc tgggtggcat
    94261 cgatataggc ctgaagggtc ttcatttccg gggcgtacca aaacccgttg taaacaagct
    94321 gggcgtattt ggggatcagg gaatccctca taagcattac ctcgcggtcc atggtaagcg
    94381 attccactgc ccgatgggca atccagagcg ccgttccgcc gggagtctca taaacccccc
    94441 gtgatttcat gccgacgaag cggttctcca ccatatcgac ccggccgacc ccgttggcgc
    94501 ccgcaagttc attcatgacc tccatgaggc gtgccggggt aagacgcctg ccgttcacgg
    94561 ccaccggatt gccttgctcg aaatcgatct ccacataggt cggtttgtcc ggggccgctt
    94621 ccggggaaac ggacatgaca aacatgtctt ccggtggctc cgcccaggga tcctccaaaa
    94681 tccctccctc atgggagatg tggagggaat tccggtcgct gctgtatggc ttttcctttg
    94741 tcaccagggg tacggggatg ttgtacttgg ccgcatactc cagcatcgac cggcgggagt
    94801 cgaacttcca atggggatcc ttccatgcgg agataatgcg gatatgtgga tctagggcca
    94861 tataggtcat ttcaaaccgg acctggtcgt tgcccttgcc ggtcgcgcca tggcacacgg
    94921 cgtccgcacc ttcggcatgg gcgatttcca cctgtcgctt tgcgattaag ggcctcgcca
    94981 gggaggttcc cagaagataa atcccttcat atacggcatt ggcccgcaag gcgggaaaga
    95041 taaagtcacg ggcgaattcc tctgtaagat cgtcaatgta gatcttggcg gccccggtgt
    95101 tgacggcctt ttcccggagt ccgtccaatt cctccttctg tcctacatcg gcggcgaaac
    95161 agattacttc acatttatat gtttccagaa gccagcgcac tataaccgat gtgtccagac
    95221 cgcccgaata ggcaagaaca acctttttca cctttttttt attcatgatt tctcctcctt
    95281 catgagtgtt tccaaaatcg ccttctggac atggagacgg ttttccgcct gatcgaggac
    95341 gacggaatgg ggcccgtcga tgactcccga ggttatttcc tcgccccggt gcgccgggag
    95401 gcaatgcatg acgatataat cctcctgtgc atgcgacaag aggttttcat tgacctgata
    95461 agtcttgaag actcgggagc gatgttcccg ttcggcctcc tgccccatac tggcccaaac
    95521 atcagtatag accacatccg ccttttccac ggcttcctca ggatctcggc agaggtatat
    95581 cccttcccgg gtttcgtttc tcccccgggc aagaagctcc gaatcgggtt catagccttc
    95641 aggacaggcc aaggccagct tcaggggcag acgtgatgcc gcgttgatcc aggaattggc
    95701 gatattgttt ccgtccccga tataggcaat cttcaagccc cggtaatctc ctttcttttc
    95761 catgattgtg aacaggtcgc tcaggatctg gcagggatgc agcaggtcgg taaggccgtt
    95821 gatcacggga atatcggcat gagcagccag atcttcgacg gttttctggg cgaaggttct
    95881 gatcattatt ccatccaggt atcgggacat gatcttggcc gtatcggata cggtttctcc
    95941 ccggccgagt tgggtgtcgc tggtgttcag gaaaacggcc agtccgccca actggtacat
    96001 tcccacctcg aaggatagac gagtccgggt tgatgccttt tcgaagatca tgccgagggt
    96061 ctttcccgtc aaaacctgat ggggtcttcc ctctttgaga aaccgtttca attccccggc
    96121 gcgggtgaag atgaaatcga aatcttcccc cgcgagatca tactcggtaa ctagatcctt
    96181 gttcattcct cctccttgaa gacctcgtcc agaatcgccg tggcctgatc gatgtcttgc
    96241 ttggtggcaa taaggggggg gacgaaacgc agcacattgg gacccgccga ggcgatcagc
    96301 aatccccgct tcatggcccg ggcgatgatc accgccgctt ccctgttcag ttccacgccc
    96361 acgaggagtc cccgaccccg gacctctttg atgagtttat gacgggcctg caattcctgc
    96421 atccgggcca tcagatagga acctttattt cggcattcct ggatgatacc ctcgcttagc
    96481 aagatctcca aggtagccct ggcggccgcc ataccaaggg ggttgccccc gaatgtggag
    96541 gcatgactcc caggctcgaa ggcggcggca actttttccc cggccagggt cgccccgagg
    96601 ggaaacccgt ttcccatggc cttggccatg gtgatgatat ccgggatgat tccttcctgt
    96661 tcgtaagcga agagcgaccc cgtccgtccc atgcccactt ggacctcatc caggatcagg
    96721 aggatatttc tttcgtcgca aagtttgcgc aagccggcaa agtatcccgg cgtcggcaat
    96781 ctgatacccc cctccgcctg gatcggttcc accaggacgg cgcaggtccg ttccgtaatc
    96841 atcccggcca gggtctccag gtcgttgaag ggggcgtatt tgaacccggg cagcaggggg
    96901 tgaaaacctt gctggaattt ttcttggccg gttgcggtaa tcgtcgccat ggtgcggccg
    96961 tgaaatgaag caatcatgct tatgatttcg ttccgcatac cgctgctgga gtcgttgccg
    97021 aattttctcg ccagttttat ggccccttcg ttggcctcag cgccgctgtt gcagaaaaag
    97081 acctttttca aggcgcaatt ttccgtaagc agccgggcgt attcgatctg ggcctcgata
    97141 taatagagat tagagacatg gacgagcctt tccgcctgtt cctgcatcgc ccgggttacc
    97201 gccggatggg cgtgacccag actgcagacg gcgatgccgg cgacgaaatc gaggtattcc
    97261 cggccctcgc tatcccatag tttcattccc cgacccctgg tgaaaactac gggatatcgc
    97321 cggtacgttc ccatgatgca tttatcggcg atcgatatat attccttttc attcatggta
    97381 tgttatgcct cttacattat gtattgattg aaagttgatt cctctttgat catcactgta
    97441 aatcatagac ttttgcggtt gtgcgtcctc agatgacgat ctccgtgccg ataccgcggt
    97501 cggtgaacat ctccaggaga atggcatgct tgtgccgtcc atcaatgata tgagcttttc
    97561 gcaccccgcc ccggagggcc ttcagacaac acttgacctt gggaaacatc ccgccctgga
    97621 tgatgccgtc attgatcata cccagggctt cggcgttgct catggtgttg atgagccgct
    97681 tgtcggaatc aagaaccccg gccacgtcgg taagcagtac aagcttttct gccttgaggg
    97741 cagccgccac tgccccggca acaatatccg cattgatgtt gtaggtctcc ccctgatcgc
    97801 catgacccgt aggggcaatg acaggaatga atccgtccct gaccagggcc gtcaggagat
    97861 cgccgtcaac ttcccggacc ttacccacca gccccaagtc aatgatttcc ggtggggtgt
    97921 ctttcgcctt ctcggcgctg aggagatatt tgtcggcctt gatgagccgg ccgtccttgc
    97981 cgctcaatcc caccgcccgg ccgccgtgct ggttgatgag gccaacgatc tccttgttga
    98041 ccttccccac caggaccatc tcgacgatat ccatggtttc ctgatccgtg acgcgcatac
    98101 cctggacgaa ttgagattcc ttccccaact tcttcagata acccccgatc tggggaccac
    98161 cgccatggac caccactgga tggatgccca catatttcat catcaccaca tcccgggcga
    98221 acatatcttt gagctcgtca tccaccatgg cgtggccgcc gtatttgatc acgatgacct
    98281 tattgtaaaa acggcggatg taaggcaggg cctccataag tatttccgct ctctccatcg
    98341 atttttttac cgtgtccata ataacctcat tggcaatgat tctctaaaga atataacggc
    98401 tcaaatcctc gtcttcaatg atgtcttcca acttttccct gacctgttcg gcgtcgatga
    98461 ccacaacctt ctccatcagg tcgggggcgt cgaaagagat atcctccaga agcgtctcca
    98521 taatggtgta taaacgccgc gctccgatgt tttccgtgcg gtcgttgaca atggctgcaa
    98581 cttcggctat ttccgccacc gcctcatcgg taaagctcag ttggatccct tcggtggcca
    98641 gcatttcaat gtattgtttg atcagggcat tgtgcggttc cgtcaggatg cggataaatt
    98701 cctccttccc cagagaatcc agctcgacac gaatggggaa gcgcccctgg agctccggga
    98761 tgagatcgga tggtttggag gcactgaagg ccccggcggc gataaagagg atgtggtccg
    98821 tcttcaccat gccgtggcgc gtattaaccg tggaaccctc cacgatggga aggaggtccc
    98881 gctgcacccc ttcccgggat acgtccggtc catggccgga atcgctgcct acgatcttat
    98941 ctatttcgtc gagaaagata attcctgact gctctaccct gcggatagcc gttttggtca
    99001 ccttctccat atccacgagc gtctgagcct gttcctgggt aaggatttcc agggcttccg
    99061 gaaccatgac ccggcatttt ttcttcttgg gagggaacat gttgccgaac agttccttga
    99121 tgttcatgcc catgtcctca atgccggccg tggagaaaat ctcaatcatc ggaccgctgc
    99181 gctgctctga cacctcgatt tctacatagc gaccgtcaag gcgaccttca tggagaaagc
    99241 gacgaagttt ctcccgtgtc gtatcttcct tccgcagtct ttccgtctcc ccgaacgacg
    99301 cttctttatc gtcggacatc atgtcaccga cggagcgctc caccggtttg ggtggcagca
    99361 gcacatcaag caaagcctcc tcggccagcg tcgccgcttt ggcccggaca ttttcctgtt
    99421 cttcagcctt ggccatattg atggccagtt ctacgagatc acggatcatc gactccacgt
    99481 cccggcctac ataacccact tccgtaaatt tggatgcctc taccttgagg aaaggggcat
    99541 tgtccagttt tgcgagccgg cgggctattt ctgtttttcc cacgcccgtg ggaccgatca
    99601 tgatgatatt cttcggcgag atctcttcac cgagttcggc cgatacgttc tggcgtcgcc
    99661 agcggttgcg cagcgcgatt gcaacggccc gtttggcgtc tccctgaccg atgatatact
    99721 ggtccagttt ttcaactatt tcccgggggg ttagggcctt gaggtccgga tcattgtccg
    99781 gtgcagaaaa tttcaagagc ggtttgggat taatcattgt caatttccaa ttcttcaacc
    99841 gtgatattgt cgtttgtgta gatacagatc tccgaagcga tccgtacgga ttccaccgcg
    99901 atttcagtag cggatagctc ggaaaaacgg accagggccc gggccgcggc caaggcatag
    99961 ggggctccag aaccgatggc cattacctca tcgtccgatt ctatgacgtc accattgcct
   100021 gaaatgagca gaaaatgatc cctactcacg gcgatcatca gcgcctccag atgtcgcaag
   100081 acacgatcgg tgcgccagtc cttggtaagt tccacggcgg cgcggaggag attgccgttg
   100141 tattggtcca gtttctggtc aaaacggtcg aagagggtaa aggcgtccgc cgtagttccg
   100201 gcaaaaccaa caatcacctt gttgtggtaa agacggcgaa ccttccgggc gccatgcttc
   100261 atgatggtga catcgaatgt cacctgtccg tcgccggcaa cggtcaccat accgttgcgc
   100321 ttaatggcaa tgatggttgt ccccctcatt ttcattgctt ttcctctcct ccccgccctt
   100381 gtggatgggc ctggtcgtag atttccatca ggcggcttac atttaccgcc gtgtaacgtt
   100441 gtgttgtcga aagactttta tgtcccagca attcttgaat gctccgcaga tccgctccgg
   100501 catccaaaag atgcgtggcg aaagaatggc ggagggcatg ggggccgatc tttttcgcga
   100561 tgcctgcgga cacgatatac ttgtccagaa tcctctcaac ggttcgggga tggatccgcc
   100621 ggtcattttg gcccctgatc aatggggact tgccgccgac gcctttgcgg gaattttccg
   100681 tctgtaagtc cagatagctc ctcaatgccg ataaggccgg ggcacccacg gggacaatcc
   100741 gttcccgcct tcccttcccc cgcaccttga tcagtgcttg ggtgaaatcg atgtcctccc
   100801 ggttcaggtc ggtaagctcg cgcagccgga tgccggtgga ataaaagagt tccagcagag
   100861 cccggtcgcg ccgtcctttt tcgtcgtccg gaaactcctg cgtgagcaaa tcgtgtgttt
   100921 catccacaga gagatgggcc gggagatacc gatccttcct tggggctgac atcccggcca
   100981 gcgggttgac atccacatat cccttctcaa gaagaaaacg aaagaacgta ttgagggcgg
   101041 ccatttttcg tcctatggat gttcttttca atcgttgccg gtggagggta gcgagatagc
   101101 cgcggattag aagctgatcc acggagcgga ccgaggcggt ggctaggtaa tcctccgtat
   101161 tttctccctc gctcaggaag gcaaagaacc ctatcagatc agacctatag ctcttcaccg
   101221 tccggggaga aagattccgt tccatctcca gatggacgat aaaatcctca ccggccttaa
   101281 tcagtgtctt ttcctggtca tctcgttgca acgcgactcc tacatcttgt atttaccaaa
   101341 ggcctcgggg gagaggtttt cgagaaattc cttcaatttt tcctccttca acttctccat
   101401 atcctcgatg ttggccccga tgtccacatg acgtgatttt tgtatgacca attctgctac
   101461 aaatatcggg gaatccgtgc ggatggcgac ggcgatggca tcactgggac gggcatccac
   101521 cacaaactcc ttccctcccc cacgcaaatg aatgttggca aagaaggtat tattgcgcag
   101581 atcgtgtatt tccacccgaa ccacctctac ctccatggtt ttgaggatgt ccttgatcaa
   101641 gtcgtgggtc atcggtctcg aaaaggtgat cttttcaagc tgggtcgcga tagcactggc
   101701 ctcgaagagg ccgatccaga tgggaatggc cttcttctct tccatatctt taagaatcac
   101761 gatcggcgtg ctggtaatgg gatcgatggt caatccggta atcttcattc tgataaacat
   101821 cggttcctcc ctcatcattt ctgaacaaga atcacctccg gtgatttacg accaagaatt
   101881 acctccggtg atttatgaac attaatcgct gccggcgatt tcagttcacc gcgcagggaa
   101941 tgcagataag cccccgtaat gttcagagta accgttttcc cccggaggat cggggggccg
   102001 gcgaaattga ctattttatt ggtccgtgtc cggcctgtaa gttcttgggg atcgttttta
   102061 ctggccccat ccaccagcac atctacatcc ttgccgacca gggcctgatt tctttcccag
   102121 gtgtggcgat cctgaagctc ctgaaggatc ttcagccgac ggttcctctc tttttcaggc
   102181 accttctctc caaatgatac cgccactgtg ccctcccttt ccgagtacat gaaggaaaac
   102241 agattatcaa agcgcacttc ttccataagc gccagggtcg catcaaaatc atctgccgtt
   102301 tcccctggaa agcctaccat gacatcggca ctgatggcga tatccgggca gcattgccgc
   102361 aaggcggtaa tacgttccag ataatcctta cgggtatagc cgcggttcat gcgcgtaagg
   102421 acccgatcag agccggcctg gaatggcagg tgaatatgtt cacagagttt tttgagcctg
   102481 ccgaaacagt ttaccaatcc gcccgtcaga tccttggggt gtgaagtggt gaatctgatc
   102541 cgttccagtt cggaaatatc gttgatcatt gctaataatt taggaaagtt cgtttcttcc
   102601 ggcaaacccc ggccatagga gttgacgttc tggcccaaaa gggtaatctc cctgatgccc
   102661 tgtttcacca gcgcctcgat ttcccggata atctccgccg atggacgact tctctccctg
   102721 ccccggagat aaggaacaac gcagtaggtg caaaaattat cgcaaccctg catgatcgtg
   102781 acgagggaat ggtgttttgt ttttccgttc ccattttttc cggcggtaca gattcccagg
   102841 gacggaattt cttcgcgaag ttccgtatcc ataacggggc cgtgtccggc cctgaccatg
   102901 tcgacaagtt gcggcacctc atggaaggca tgggtcccga aaaccagatc cacgtatggt
   102961 gtctttgtaa acatgtcccg accccagtgt tgggccagac aaccgcctac accgatgatc
   103021 acctcggggt ccttgtcttt ccaccgccgg tagcgcccaa gctgactgac tatcttttgg
   103081 gcgactttag agcggatgct gcatgtattt accaggatga tcttcgcttg gcgtacatcc
   103141 ggggtctgct catagcctgc ctcggtcatg atgtttgcca actgctcgga atcatggacg
   103201 ttcatctggc acccaaaggt gtggataaag aaccttctgt cagcaaaatt ttccctgccc
   103261 atgaaagcga cactcgatgt tcaaaaatac gtaaaaaagt tgactattat aagggctcat
   103321 cagagtcaac agttttttac ttgttggcgg tttttatccg gcggcccacc cattcctgca
   103381 agacaatgcc ggcgacaatg aacagcagtc caaaaaagga agcggcgcgg accgcctcgc
   103441 cgccgatgaa gtgtatgaat accagcgata taaacgggga taggtagata agacgggtca
   103501 cctggatgga gctgcgggaa agggtcaggg ccttcatcca gacgaagaag gttatcccca
   103561 tctcgaaaat gccgatatat gccgcccctg ccagtcccgg ccaggacggc cgacccagct
   103621 tggccaggca tatgctcagg gtaagggtat agaggaatcc gaaaaagaaa ttcaaaaaga
   103681 gtttcacccc ctcctcccgg cgatccctca ggttggccag ccaataggaa gcccagacta
   103741 tggaactccc catggccagc agcgccccgg ggagattggt aacccggaaa ctgatgagat
   103801 cgccccgggt ggagatgatc agaataccga taaaactcac caacatcgtc atgatacgca
   103861 ataaatcgat acgctccctt cccgtcaaac ccgccatgat aaccagcatc aacggccagg
   103921 tatagttaag gggctgggct tcctgggcgg gaaggagatc ataggcttta aaaagaatca
   103981 aatagtataa aaaaggatta aggaacccca gaacaagaga atgaagataa tcgcgccggg
   104041 tacagcgtcg aatgagcggc attttcccct gtaataccag gactatgccc aggataagcg
   104101 tcgaggtgaa ggaggcgtaa agaagcatct ctaaggcgga aagatagcgg agggtaattt
   104161 tgaaggccgt agccaccgtt gaccagaaca acacggtgag gaatgcataa acgtaggcct
   104221 gccgctgctg gggcattacc cgcttctcca tgcaccttta tccacggccc gccggaggga
   104281 tgattgcatc gaaatgatct tttcccaaag catggccata aatctctccc ggccggttct
   104341 tctctccccg gcctctccag gccgtgaatc ggcgcaagat tactgggcat agattagaca
   104401 cgctgtattt gattaccata atcattggaa tattgacaat gtcatttgaa tattttccct
   104461 aatgaaactc ccggctggac atttcttggc agatatgcca aatcatgctc cgtttcattt
   104521 aacttacggg agggattgat ggagacagtc gatatcctca tcaccgacgg tcgggtcgtc
   104581 accatggacg aaacggatac gatttatgaa aacggcgccg tggccgtcgc acagggaaag
   104641 atcgttgccg ttggccttac ggatgaactt aagaaacggt tctccggtct ccggacgatc
   104701 tcggcgcgca atcacattgt catgcccggt cttatcaaca gccataccca caccgccatg
   104761 acctgtttcc ggggtttcgc cgatgacatg gaactcatga cctggtggaa ggaatacata
   104821 ttcccggcgg agacccgtac tgtttccccg gagcttgcct attggggaac cacgctggcc
   104881 tgtgcggaga tgatcagatc cggtaccacc accttctgcg acatgtattt ctttatggag
   104941 gagacggcgc aggcggccag aaaagcgggg atgcgctgtc tgatcggtga agtcctcctg
   105001 gatttcccct cggccaatgc caaatctccg gcggaggggc tggaatatac gcgaaaactc
   105061 atcgaaaaat ggtccggaga ccccctggtg cggattgcgg tggaaccgca ttccctttac
   105121 acctgctccg aaaatgtttt gaaggaggcc cgtgccctcg ccgtcaggta taatgtcccc
   105181 gtagccattc acttcctcga aaatcaggcg gaagtcaaac aattaaccga gaaatatggg
   105241 aaaaaggcaa ttcactactt gcgggaaatc ggctttctcc acgacaatct gattcttttt
   105301 cacggcgtct gtatggatga cgaggatatg cttatctgtg ccgattacgg ctgtaaagta
   105361 gtacacaatc cggaaagcaa catgaaactc gcttccggcg tggctccggt gggaaagatg
   105421 ctggcccggg gcataacggt ggccctcggt acggacggct gcgccagcaa taacaacctc
   105481 gacatgtttc aggaaatgga cacggcagcc aaactggcta aggtggcggc cctggacccc
   105541 acaatccttc ccgccccgtt ggtggtgagg atggcaaccc gtaacggagc cctgaccatg
   105601 ggattggagc aggcggcggg tgcactggag gtggggaaaa gagccgacat cattttgatc
   105661 gacatccgga aaccgcattt gacacccctg tacaatgagt attcccatct cgtctacgcg
   105721 gtaaacggtg cggatgttga aacggtcatt atcgacggcc gaccggtgat ggaagatggc
   105781 cgcctgctca ccatcgacga gaaagaggtg atgactcggg tcaacaaaat tgcctcccag
   105841 ttaagaggaa gcttttctta agcaatcgag gtaagaaaaa tgcagagttg ccgcgctccc
   105901 cttgatatcc ttatccaggg ggggactctc ctgaccatga gcggcgggga agaaatcatt
   105961 accgaaccgc agttaggaat tcgggacggc aggatcctct tcattgcccc cgcccatgac
   106021 cctctggcaa ctctctacgc tccgcaggaa gttattgacg cttcgggatg tcttatcctt
   106081 cccggtctcg taaacaccca cactcacgtc ccaatggtct gttttcgcgg tcttgcggac
   106141 gatcttcccc ttatggactg gctcaacaac tacatctttc cagcggaaag aatgttcgtc
   106201 aaccgggaaa tggttcgtgc cggtgcgttg ttggggattg cggagatgat actttcgggg
   106261 acgaccacct tttgcgacgg ctatttctac gccagcacca ttgcccggac cgcccaggag
   106321 tccggcgtcc gctgcgtcgc cgggatggga atcctggatg ctgatatcag cctggttgag
   106381 gaggcggaag tcgccgcaca tgtaacggcg gcggagaaat tcctgaccaa atgggcggaa
   106441 gcaagaccga cgattatccc ggccctcttc tgccactccc cttatacctg ttccccccag
   106501 acactacggg ccgtcaagga ggtcgcccga cgtcatgatt cactttttct gattcatctg
   106561 gcggaaacgg cggaagaaac agacatcatc acggcccgct acggtaccgg tccagtattt
   106621 catcttgagt cactgggcgt tctggatgcc aggacagtgg caattcattg caactggctc
   106681 aacgaagagg agatcgattg cctggctaaa aatggtgtca aggtatcaca taacccccag
   106741 agcaacatga aacttgccac cggggttggg cctgtacctg ctcttatcgc cgccggagtc
   106801 accgtcggcc tggggaccga cggttgtgcc agtaacaaca atcacgacat gttcggagag
   106861 atggacgggg ccgccaaact acacaaggta ttccataagg atcccaaggt gatggccgcc
   106921 ccgcaggtgc ttaagatggc cactatggac ggggccaggg ttctcggtct ggaagatcag
   106981 atcggttcgc tcgcagtagg taaacgggcc gacatcatca tcgtcaatct tcagaagcct
   107041 catcttaccc cattgtacaa tcccttttct caccttgttt acgccgcctc cggcgccgat
   107101 gtgaggacct ccatcatcga cggtaaaata gttatgaaag atcgacggat attgaccatg
   107161 gatgtgggag cggccatgga tgccgtggcc gacattgcgt cggccattag gaaaagtcga
   107221 tagaagatat tagatgtcca ctaaatacag ttgccctgaa taaggagaaa aaccatgaga
   107281 aagatatttt ccctttttgt tgcaggactt gtgatgatat gggtgacgaa ttttcttctc
   107341 acgtcatatg cgattgccgc accgttggaa gtgacgctgt atccggcatc agcacatatc
   107401 accgagcttc agaagctgcc gctgaaggcc attgacggca aaaccagcga agccgtattt
   107461 ctcataccgg gccagtccga cccggaaaca ctgacgacga agattgtcaa cccggcaaac
   107521 ttgaaggttc tggaccagcg ctggcgccag gtcacccggc aggatgaaca ggcagtgacg
   107581 ctcttgagaa aggagcttgc agacctcaag aaggaacacc ggcgcctcca ggccgatatt
   107641 cgttccttgg aaacgcagat ccagttctgg cagttgcaga cgaaggtccg gatgaagacg
   107701 gtgaccgagg ccggaaatat gtcggccgct atcggtaaga acatgagaaa aacggtccag
   107761 gacaagctgg ccctggagcc gataatcgat gacataacga agaaaataaa ggatctcgaa
   107821 gaaaagctcc agaggacgac gggggaaaaa gaaacctcat gggaagtgac ggttgtttta
   107881 tccggagccg ttgtcaggga ggcgactttg gcttacggct ataccgccac gggctgcggc
   107941 tggtcctcca tgtaccgttt ggaggcccag cccctgaaac accgcgttcg ctttacctgg
   108001 gacgcgcaga tctggcagag taccggacag gattggcgac aggtcagcat ccgcctggct
   108061 accctgcaac cgcggaagat catcgaaccg ccggaactgc ctccctggat catcaaaccg
   108121 cgggagcggt tgatctatcg caatcagaaa agcgcccggg gtgcaatgtt gatggaagcg
   108181 gcggatcaag cggagctggc gtcgccggag gggggagcgc cgaccctgac cagggaaagc
   108241 tcttttcatg tctgggagat tgggaaacgg tcccttgccg ccgggcagcg ccaaagactg
   108301 caggtccggg aggaggaatg gccggtggag ttcctgcatc tgaccaggcc cgctcagggt
   108361 gaacagacct ttgtgcgggg cgaggtgaag ttttccgaag accgggaaat accgccggga
   108421 atggcattgt acatcatcga cggatcattg gtgggcaagc gttccttctc gctggcgggt
   108481 cgggagggga ctatctactt cggtagtgat cccctggtca aggccatggc ggtcctcatc
   108541 aacaaggctg cgggagaaga aactcttttt aacgacaagc agacctatcg ttggggctgg
   108601 cggatcgaaa tcagtaatgc ccggagttac ccggtgcggg tccggatcga ggagtccctg
   108661 ccccagtccc gggatgagcg gattcagatc aacctgaaga gcgatcctgc cccgacggaa
   108721 accactccca acactcagat ctggatcgtc gaggcgccgg cgggcgggaa aaggcacctg
   108781 ctcaccacgg tatccctgga ggcaccgaag aatctgccgc tggatttggg ctggcgacgg
   108841 tgaaaaccgc tctgccggca tgggcgatga agagacgttc cgggatattc agattatcag
   108901 attgaatttg acaccgcccc tttatcaggt aaggggttga tcgccattga ggcgctcgta
   108961 gataaggata tactcggcga tcatggttcc caggtcgtat gcctcccggg cctgcctcat
   109021 gatgcgtttt agctgtcgtt cccggacatc ccggggcttg cgatgaaact ggacactctt
   109081 ttccagggcg taccagagcc cgccgcggtc ataatcccgg aaaaggaaac cattgcccac
   109141 gtcctgggga gagccatctt ccttcaaccg tagttctctg atcttgtcat ggtagccacc
   109201 ggtgtcccga ttggtggccg tggcgccgaa aaggttgcct acctggtcga tctgaccgca
   109261 gggctcgtat agtgacgccc cgaacacgtc gctggccgcg gcatatccca gcatggaaag
   109321 cgattcggta aagggatgat aagcaatgcg ccctcccgaa gtataggcga tccggcctag
   109381 aatatccaca tgggtccggt cgcttcccac tccatcaccg acaatggcga tctgcacatc
   109441 cgagtaagcg tcgacaaaat cctgggcgat ggcctccaag agctcgacac ctttttgaaa
   109501 agggtcgagg cgggagggcc aatagaagag gatggcgccc ggatcgatgt tcaacccgag
   109561 gcggcgctgg aattcccggc aattttcctc cttggcggct agaatttcat cttccacgcc
   109621 gtaggtcctt accagatggg gacagttttc aggatacata ttcgaggccg gggcattgat
   109681 aatggcccgc gcggcgccat attgatattt tgccttcacc tcctggcgga cgctggatgg
   109741 gataatagga cggtccatga agtagtcgtt taccacttcc cggaggaaac gttcgccgac
   109801 aaaatttatc atggtggcat tcttgatggc cgtggcctgg caatcgacgc actgccggcc
   109861 atgatcgaag gatagataga gattctgggc ataattctgc caccctaccc ctcccagatg
   109921 ttccagaggc aggtgagcgg tgaagacgtt atggacggta tggaggaccg gcagccccgt
   109981 ttcctttgcg taggcggcaa tgaccccgcc cgccatccag tcgtgactgt ggataatgag
   110041 cttgccctga tgctgggccc gaaccgtctt tatgacattg ttgacgatat cacgctgaaa
   110101 ctccgccgcc gtgaggaggg gattgccggc ataggcgctc atactgtcgg cgaagattgc
   110161 cgaactcacc agatggatgc gcttggggtc gagcttgtaa cgctgttccc gccattccca
   110221 ttcgtccagg ttagattccc gctggaagcg gcgtttcagg tttaaggtaa ccagatggac
   110281 attgattccc cgctccgtca tcccctcgca tagggccgaa accacttcgc cctggccgcc
   110341 gcttttcccg gagatatact tggccagatc gcccatttcc tccggcagtg gtccggtttc
   110401 cggagtgatg attagcaccg cggtcttctc tgtccgcttg atgatctcaa agcgagcaag
   110461 atcgctcatg agttgatcga tcttacgggt caggatgtag gtcggttgag atatggaacc
   110521 gccatagggg ttgaaatagg catggacggc gtggtcttcg ccgatgctct cgtggagaaa
   110581 atagacgtga tcggaggtgg tgagatggcg ccagcgggtc agcaaatccc cgcccgcctt
   110641 tcgggcgtcc gcttccatat tttcgatatc cttgaataat tcatactggg tcatattgcc
   110701 cagccagccg aaggtgtccc gggttgcatc ggcccaggaa gaggtcgcta ggccgtcaat
   110761 atccaccaca ggacactcca cctcgcgaaa atgctcggct atctccgagg ggttggccat
   110821 gactatggac ggatacaggg ccagtgcggc gggcagctcc cgccagaagg caaagatccc
   110881 cttgtcttcc cagatatgct cgccgatatg ttcgtaatcg aaaccaagaa taaccgcttc
   110941 accgtcgata cgggcgatga aagaggcgta catctccggt gtcaggggac gatggggaaa
   111001 ccggaaggca atgtcgtcgc tcaattcgcg gtggcggggc aagacgacga ggctggatcc
   111061 ccgggcgcgg aagacggcgt tgggggagat gggggcgcca ccattttcaa acatgtcatc
   111121 acgcttttca cagagaatgg cccgataacc catgtccgcc accacggcag cgatattgtt
   111181 attgtacatc agctccgtat ttcgaaatgc ggacgggaaa aagccgaaca ggcggcggat
   111241 cttgtcccga tggagagaca cctggtcgcg gaattccctt tttgccaggt ctttaaaaag
   111301 gctcgtcaaa gaatggtagt acgtttcatc gagaaactct agctgtcccc gctcttgccc
   111361 ggcatccacc agtccctgca gggccttgat caactcaggc tgataatttt ccgcctgttc
   111421 gagaaacacc ccggacatgc tcatggtgat cttgaatcct ggataggctc taataagctc
   111481 cgtaaagagg taggtggcgg ggagataaca cttctccgct accttgatga agatatcccg
   111541 gttttgtttc tcccagagaa acttgtcccg atctgggtgg aggcgaaagg gctggtgaag
   111601 ttgaaaatac acggtgagca acggcatgat tcaatcgacc cctcattatt gaaaaagaag
   111661 cttaaggccc ataagttgtt gacgctttac agtctgtttc ccgccgaggc tatatgatga
   111721 tccatgctct ccagacgtcc ttgagatccg tagacggtcg gaaaatttta ctctaattcg
   111781 agttataatc aagaaaaaac cggctattta tgctttagcc ctcgtcatta ttgacaaccc
   111841 caatctattt gtgaaatata tcccaggtga ttaaacggca tctaaaacta caagatcttt
   111901 gtgaaaagcg gcggattcta agacccgcaa cacgctaccc ggcccaactg cccattactg
   111961 catgcagaga agagatcgtt gcggcaatcc gccggcatca ggtgctgatt gttacggggg
   112021 aaacgggttc cggaaaatcc acccaattgc cgaagatgtg cctcgaggcc gggcggggca
   112081 tcaggggatt gatcggctgc actcagcccc gacgggtggc ggcggcggcc atcgcccgcc
   112141 ggatcgccga ggaattaggg gaagaattgg gcaactccgt agcctacaag atccgctttg
   112201 acgagcgaag cggcccccat ccttacatca aggtgatgac cgacggcatc ctcctcatgg
   112261 aggcccagag tgaccgtctc ctccggcgtt acgataccat catcgtcgat gaagcccacg
   112321 aacggagcct caatatcgat cttttgatcg ggttcctcca gactattctc ccccggcgcc
   112381 gggatctgaa ggtgattatt acctcggcga ccctggaaac ggaaaagttc gcccaagcct
   112441 taggtaacgc cccggtcatc aaggtttccg gccggctctt tcccgttgag gtacgttggc
   112501 ggcccccggt ttcggaagat gggctcgatg acggcggcgc ttacgcggat ggagcggtta
   112561 ccgccgtggc ggatctggaa cgtaacccca gagaaggacg atggggggac atcctgatct
   112621 ttatgcccac ggaacaggac atccgggaca cctgcgctat gctcgccggt cgcttcggtg
   112681 acgacaaggt cgtggtgccg atgttttccc gtctctcctg ggaggaccag cagcgggtat
   112741 tccgacctga ggaccggcaa aagatcgtgg tggccaccaa tgtcgcggaa acatccctga
   112801 ccattcccaa cattcgttat gtcatagata ccggactggc ccggatctcc cagtacaacc
   112861 cccggtcgcg gaccgccggc ctgccggtga cacccatttc ccagagcagc gccgatcagc
   112921 gtctcggtcg ctgcggccgg gtgggaaatg gtatttgcat ccgcctgtat agcgaagatg
   112981 actatctttc ccgtccggcc ttcaccctcc cggagatcaa acgcgccaat ctggccgggg
   113041 ttgttctcag aatgctcgcc ttgaacttcg gcgatatctt cacctttcct tttttggagc
   113101 ctccggataa gaaacagctc cgggatgccc tggatatcct cagagagctc ggagccgtga
   113161 ggatgggaga ggggccgaca ctcaccgaaa tcggccgctt catggcccgc ctccccgttg
   113221 acccccgcat ggcgaggatg cttgttgccg cggcttctca tgactgtctc gaggaaatgc
   113281 tcgtcatcac ctcggccctg agtatcgtcg atccccggga gcgcccccgg gatcaggcgc
   113341 ctctggcggc ggagatccat ggtcgctttc atgatccgac ctcggatttc atcaccttcc
   113401 tcaacatctg gaaacgctgc caaatggtgt gggaggagtc gggatcgcag ggacggctcc
   113461 gcaaattctg tcgggatcat tttctctcct ggcggaggat gcgggaatgg caggacctcc
   113521 accgccagct ccagactatg accggggagg tactggtcga aggccgtcag ggagaggacc
   113581 ataaagctga agtcagaccg tcccgtctcc tgcgccatct cccggtgcgt agccagggtc
   113641 caaaacgagg ccgggaagac aaaaatgctt cgattcacaa ggccgttttg actggttttc
   113701 tttccggcat tgccgtccgg aaagaaaaaa acatctatac cgccccgaag ggtaggacgg
   113761 ccatgatctt tcccggatcg ggcgtgttcg gtacaggagc ggattggatc gtggctgcgg
   113821 aatgggtgga aacctctcgg ctttttgccc gtaccgtggc cgttgtccaa ccggaatgga
   113881 tcgagggggc cgccggagag ctgtgccgca ccgtctattc cgaaccttat tggaatcggg
   113941 agcgggggga ggttcaggcc tctgcccgga tagtcctttt cgacatggtc atcattcctc
   114001 accggaccgt ttccttcggc cgcatcaacc cggccgccgc ccgggagatc tttatccggg
   114061 aaggaatcat gacgggatca cttaaaaaga gctatccatt cctaagccac aacgaacagg
   114121 ttttggaagg ggtgcgcgtc atggagaaca agctgcgccg ccggggatgg ctatggaatg
   114181 aagagacggt gtttgagttt taccgccggc atcttcctga ggagatatgc gaggaacggt
   114241 ccctgcagag atgtatccgc caacggggca gtgatgaatt cctgtggatg gaacaaactg
   114301 atgtgcttct cgcggaaccc gatgaaaatg cactgcggga atggccggac gcggcaactg
   114361 tgcagggcat ccccgttccg ctaatataca actacaatcc cggcggtgaa gacgacgggg
   114421 taacggcccg gatcatggcg ccgctcctgg cggcggacga gacggagagg ctcgccgtcg
   114481 ccataccggg gatacgcagg gaaatcgttc gggaatacct tcagagcctt cccaaatcat
   114541 accgccggcg gctcccttct ctgcgcgatg ttacggacac gattctcatg aatttgaacg
   114601 atcctgatcg acctctgccc gaagtcctga gccgtcttct gaatcggcac tatgatttgg
   114661 atcttcctat aaccgtatgg caggaaacaa aacgtctggg atcaggacag atccgctacc
   114721 tactaacgga cgaagaaggg cgggaactca gccacagcag aagcctgccg gaactgcggg
   114781 agcgttatct cgaaaagatc aaaaaggaaa cactgcataa cgccaggaaa caatgggaaa
   114841 ttgagaatat tcaaagttgg gatttcaatg acttgcccga atcgattccc ttgggcgatg
   114901 ccgttctgcc ggtggatgtc gtttatccgg ccctgcgcga cgaggatggg atcatttgcc
   114961 tccgtctttt agactcccct aaagaggccc tcgcggtaca tcgacaaggg gttgccgccc
   115021 tctatgagag acactttgca gcggaatgca aatatttgaa gaaagttctt ctcccggcgg
   115081 gaaacatgaa actccacggg gaaaagttcg ggggatcgaa aaaggttcag aggatcctat
   115141 attctaaagc attacttgat ttattttttg taaatgtaag aactaatata gattacttaa
   115201 aacatgccgc ggaagtaaaa agcctcatca tccctcatgc caaggtagtt ttgcagctcg
   115261 cggcaccggt tttggaggca aatttccagg ctgagatcgt tttggatcgt ctggagaaac
   115321 agtacggctt ctccaaacca catcgggaac atctcacttt ccttcgccgc gaacttgcct
   115381 gcctgattcc cgaggacttt ctgaaccgct atcccgcgga acgaatccgg accttgtccc
   115441 gttatatccg cggtttaatc attcgggccg aacgggcgat gctcaatctc gataaggccc
   115501 tggcgaaggc cggcgaggtg gatgtttatc gcgaacaatg gcggcagttg actgtcggct
   115561 cacagcattc accatctctg gcaaagatgc aggcccagga agattttttc tggtttctcg
   115621 aggagtacaa gatctccctg tttgcccagg agctaaaaac cacggtcaga gtctcaccta
   115681 aacgtctgga ggaaaggctc agggaactcc gggccatgct ctaaagaaaa aaatggccac
   115741 attaataaga tcatgataaa aaggataaaa tttcatgacg gaggcaaggc gatgtccatg
   115801 tccataattt cccggctcag tattaaaagc cagggtctgc aatataaact catcatcatc
   115861 caggggctcg tcttcgttct tcccttttgt ctcctcacct atatccttta catcaagaat
   115921 gcttttcaag acaccgaaca tgtcgtggcc tatttgttga ttctctttgt ggttttggcc
   115981 gggatcgtca ccctgcggca gattttcgac cgcatcttca aatttgctca acatgtcgag
   116041 caagcagcgt tgggctacgc ttcgaccagc acccgggagg atttggatga cagtgaggat
   116101 ctgaaggaaa taagtcattc ctttcagacc ctccttgaca agttccagca agttggggca
   116161 gaattgacca agaagaccgg cgatcttatg gcgatcaagg aactgacgga aatggccgat
   116221 catcgtctcg acctgtcccg actcatgcaa tcccttaccg acaaggccct ggaggttgcc
   116281 acagctgcgg ccacggcggc atattatttc gagaaggacc aggcgctccg tataatcccg
   116341 gcctccggca gtgatttaag tgaggattac caaaaatttt cctacgatat ggaatgccgc
   116401 gccaggctgg ttcttttaga tatgaaggcg agcatctccc cgacggacgc ccataccttc
   116461 tccatgatct ctttgcccgt agtggatgaa gaagacatta aggctattct catcctggtc
   116521 cgcagaaaga accagaagcc tttcggctcg gaagatctgg attctctggg catcatgctc
   116581 aatgccgtga aatcgaccat caagaacgcc ctcctttacc ggcgctccga agatcagcgg
   116641 gccatgttga aggaaaaaag tgaatttctt gccgttgaaa tcgaaaagag aaaaaccgtt
   116701 gaggattcgt tgaggcaaac caaggagaag tggcgtcgat attccttcat cgtcaacacc
   116761 gcaaaggaat ttatgaccct tatcaacagg gagtatcatt atgaaacggt aagccagtca
   116821 tattgccatg cccaccaaaa gagcccggaa gagataatcg gcaagagcgt tcatgagata
   116881 tggggacccg gcagtgcgga tcttatcgtg aagaatctgg atcgctgttt taccggtgag
   116941 gaaatatact atcaggaaca attcgccttc aacgacggcg tgagtcgtta ctacgacgtc
   117001 aattactacc cttaccgtag tgtggatgat ggtaagatta cccatgccat cgtgatcacc
   117061 cacaacgtga atgattacat ggtcaataaa atcgatctgg aaaacacggt cacaaaactc
   117121 cgggaactca tgaagggcat tatcgccacc atctcgaacc tcatcgaaat gaaggatccc
   117181 tatacggcgg ggcaccagca atccgtggcc aacatcgcct acgccatcgc cacggccatg
   117241 aatctacccc aggagcaaaa ggagctggtg cgaatcagcg ccgagatcca cgatatcggc
   117301 aagataagca tccccgcgga aatcctaagc aaaccaagca agctcaatga gatggaatgg
   117361 gcactgatca aagcgcatcc ggaaatgtct gaattgattc taagcaagat cgactttccc
   117421 atccctatcg cccccgttat tgtgcagcat catgaacgga ttgacggatc cggttatccc
   117481 cgggggttga agggtgacga gatatgcctg gaggcaaaga tcatcgccgt tgccgacgtg
   117541 gtggattcca tgtcctccca tcgtccctat cgacccgcgg tgggccagga agaggctctt
   117601 gaggaactca aaagaaaccg cgggatcctc tacgacgcag cggtggtgga cgcctatatg
   117661 agcctagatt caacagcaat taatcctgat tcacgaccag atcggtgcat acccggatga
   117721 aattcaatcg ccctatctat aaaatagtca taatataacg cgacttttcc gtgtttgtaa
   117781 acaatgttgt tgtccctgat cgttgttcag gaatactggc gtagataacg acggatgtat
   117841 ttttccacgg cggcccgggg ctgaaatatc ccgaaatggc cctcgcagag gatatcggca
   117901 ttgagctcaa gaagcttttc catggattcc cgccactgcc gcacatcgga acgaaacgcc
   117961 ggcagaaacg gtccgtggat atcctgccca aagaggatac gcttcctgtc cctatccaaa
   118021 taaagacata tggagccggg agtatggccg ggggtatgaa gccagtgcaa ttcatgggga
   118081 ccgcaccggt acactccctt ggcgccggtc aacttttcat ccaccgatag gggcggaaaa
   118141 tccatttcat accagttggc agccgtcatg attggatccc cggtctcgac ggccgcggca
   118201 tcgtcggcat ggatagcgat acggcatgca aactcacgct ggaaatacgc tgcggcaccg
   118261 atatggtcga tgtggcagtg ggtaagaatc agcaggctta tcttttgggg atccaggcca
   118321 gcatcaagga tattgctttc caggattcgg gcgctacgac cggcgccggc atcgatcatc
   118381 accagttcat cgccgccatc aatgacaaag accgtggcgt cttctgagcg ggagatattg
   118441 ggacctccga ctagatatat ccccggcgcg atagtttccg ctgcccccat gaagacttcc
   118501 tcctttcaag tatgtagagt gcccaccagt tcccttatgt ttattttatg ccggtccata
   118561 taatctctga tgccggccag tatctccatt gtggctttcg ggtttataaa gtttgccgtt
   118621 ccaacttgga tggcagtggc gccggcgatg atgaattcca aagcatcggc agcggtcatg
   118681 atgccgccga cgccgattat ggggatattg acggttcgcg ccgcctgcca gaccattctc
   118741 agggccactg gtttgattgc cggaccggac agccccccgg tgatattttg caaatggggg
   118801 atgcgtcttt ccacatccac ggacattccg gtgagggtgt tgatgaggga aacgccgtcc
   118861 gccccggcgg ccgccgccgc cgcggccata acggtgatat ccgtgacgtt ggggctaagc
   118921 ttcacaatca acggcaggtc ggtgatacct cgcacccggc cggtcacatc agccaccata
   118981 tccggatcgg ttccgaaggc gacgccgcct ttttttacat tgggacagga gacattgatc
   119041 tcaagggcgt gaacccccgg cgctgcggtt aaaagttcac tgacccggag gtattcctca
   119101 accgtttctc catagacatt ggcgattacg gcgatatcga aatttcgcaa gaacggcagc
   119161 ttcttgttga taaaatattt tacgccgata ttctgcaggc cgatggcatt gagcattccg
   119221 gcgggcgtct ccatgatccg cggggaagga ttgccggatc gggccttcca tgacaggccc
   119281 ttgacgacga tggcccccag ggaattcaga tccacatagg gagcatattc ctcaccgtag
   119341 ccgaaggttc ccgatgcggt catcacgggg tttttgagac gcagtgaccc gatttccaca
   119401 tccatgctaa gattcacact attcacacat ccctatcgcc tgtcgtatcg atttgcaggc
   119461 acattcagag gtcctcccaa catatctccc ggagatcgaa gaccggtccg tcccggcata
   119521 cagacttctt tacaatccgc tttcgggacg ccagcaccga tatggcacag ccaagacagg
   119581 cgccgacccc gcaggccatc ctggcttcga ccgatacctg gcaggtaact ttcgcgggca
   119641 ggcaggaagc cagtgatttc atcatgcccg gagggccgca ggcataaaga accgccgcat
   119701 ctgcttcaca gcgaccgaca atctccggca gcaactccgt caccgtcccg caggttccca
   119761 aactgccatc atccgtggat atatggaact ggccgccgat cttttccagt cgttccaggc
   119821 cgataaattc cccgctgttt ttcgccccca ggcagaagac catccggttg ccgtcggagc
   119881 tccggttgca gagccaagcg gccagatagg tgatcggggc gatgcccatt cctccggcaa
   119941 tcaggatgtg attccgcttc ttttcgtcga tgtggaaacc gttccccagc ggtccgagaa
   120001 cacggacctc ctttcccgat ggccaccggg aaaacaatgc ggtgccccgt ccgacgatcc
   120061 gatagagaag ctccaggacc acatggccat gaaggcgggt gaaatgatag acgctgaggg
   120121 ggcgggaaag gagcggtcca tccgactccg ccgttctcac catcacgaac tgtccgggcc
   120181 gcggctggac aaattctttg ggcagccgca gggccagatg aaaatcacgt tcgttgaggc
   120241 ggtggttgta gatcacatag gccgtgtaca tgtatcagcc acccctttcc gcttcggcga
   120301 catttagaat atcattcacg gcgccggtca acaacagtgc atcctcacct gtttcccggt
   120361 agtaaccgcg ccttcggccc caagttacaa atccgaaatt ttcatataac cggacggcct
   120421 gtatatttgt ctgccgcacc tccaattgta ctgtagagac ggagcgctca gacaggtagc
   120481 ggagcatgtg ccgaatgagc ccggcggcga tgccccggcg gcggaaatct tctttaacgg
   120541 ccagacggtg cagttgcgct tcatcgataa cgatccagaa attaatatat ccaaccaaaa
   120601 tatttccgcc gtcatccttg ccgggcagtg ccacgaaggt ccgggaaatg gtgttctgcc
   120661 gctcctgcct ccacgtcgcg gcggtatatg gctccggaaa gcagcaatgc tccattgcct
   120721 ccacttcttc tatgtcaccc gtttccatgg ggcggatgcg gatctcaaaa gccattgacg
   120781 agttcgatga tgatcatcgt cgttagcacc acggcgcaga ataacggtac cagccgtcga
   120841 aaattgatgc tgttgaggca tacggcaatg agcaccaagg gtatgattcc atgcattagc
   120901 ctcaaagcct ggaggatatt ctctggcaag accgggaaaa ttgcccggag ggtcaggatc
   120961 ccgacgcccg aggcgaggaa gaggaagatc acataactgg tcagggtccg ataaattgcc
   121021 cggcgatgct cccggccgat cgtctcgatg tctccggagg aagccgcccg cagggcgttg
   121081 tccgataggg cttcgttggc ctggatcaag gcggtgtcca ttcttttggt caaataactc
   121141 attggtagca atgccaggaa ggcgaaagcc gtaagttcct ggcgtcgttc ggtaagttga
   121201 ggaaaggcca gcagaagcgc ggttacgatg acaatggcga gcagcgagtc gttcggcggc
   121261 acatagatgc cgatttgctg cttgtccacc cacaagagct ccaaaaaagc cccgatggtg
   121321 agaccggtca gtacatcccc cagcacccag ccgaacagcg gggcgacgac caccgggcgg
   121381 gaaaccatga tttgcaggca ggtccgatcc agacataaaa aaccgcccag tattgaaacc
   121441 gccgtcattt ccagcagcat tttattggct tccgaattgt ttttataaaa tttctttcag
   121501 gacctccgcg atattgacgg gtcgctctct gggaacacgc cggagctcga tattcacgcc
   121561 tcgctgcagc agactacgaa tgctgtccag atcctcatcg ttcagtaaga cgcaggccga
   121621 aagcctttga cgacaatcat cattgtaaat attgccgata ttgatctttt cgaagacgaa
   121681 gcccaaagcg taggcccgca gggcatccca gaccctggaa aagaggacga tcgatcgcgc
   121741 cccgcccggt gcctccccaa gcgcttcgcg gacgaaatcc tcgacgctct ttatcttcac
   121801 gaccatttcg ctgggcaccg ccatcttgat gaccgtctca cggaaaaagt cgcttgccac
   121861 ctcgtcatcg acgacaatga tctgagcaat ttttaaatgc ggcacccagg cctcgatgat
   121921 ctgtccgtgg acaagacggt tgtcaaccct gacaaggacg caattcataa actcaggcgc
   121981 cgatcttttt attaagaatc tcactcgcca gataaatatt ctttatccca tattccttga
   122041 tgaaatttgc gaattctcgg agattcatgt tgctccgtcc atcggagagt tttagcagca
   122101 tgggaaggtt gaccccggtg ataacctcaa cctttccctc ttttagaaag gaaagcgaga
   122161 tattggaagg cgtgccgccg aacaggtccg tcaggatgat tacgcccttt ccctggtcga
   122221 gttttttgat ggcggtactg atctcttttt ttatctcctc catccccttg gtctgatcga
   122281 cggagatgtg ggcaacccct ttcaggctgc ctttgatcag ttccgccgcc ttaatcaatt
   122341 cgtgtcccag ttcgccatgg gtggtaatca gtacgccaat catgattttt ttgccctcga
   122401 ttgtcgttat tttgcgtcac gctaatggat tgggcggcaa ttgtcaaggc cttatcggtc
   122461 agctcatcac acgcctccca cgcctccctt gacaattcga gcgggaacgg ttatagactt
   122521 tttaaacgag gcatctgcga tgtgcgtttc gagttgcgtt gctggaatcg aacacgataa
   122581 gtctgacagg gtcttagtgg tgatcgccac ccagagaagg ccaaccaaat ggaaacgtga
   122641 ctccagcctg aaaccggacg gcataggcgg acctcgtttt ctttcgtttt cttttcgacg
   122701 catcgagggg taaacgtgac aatcatatat ccttccccat gatatcgtcc ccccagaatg
   122761 taaacaaaac cgctctgatg gctgtcgtcg ccctgtcggg aatctggggc tacaactggg
   122821 tggtgatgaa ggaatgtctg cactacgcat ctcccttcga ttttgccgct ctgcgcactt
   122881 ccatcggcgc cgcgagtctc ttcctcatca tgtttatcca aggcaaatca ctttatccga
   122941 aggaatttat tcttaccctg gccctcggcc tcctttccac taccggctgc atcggtctcg
   123001 tgaccctggc gttgtattac ggcgccgtag gcaagaccgc cattcttgtc tatatcatgc
   123061 ccttctgggt gctgatcctg gcctggcccc tccttgcgga gcgtctggcc gggagtcaat
   123121 ggatggcggt gagcctggcc tttgccggtc tgatggtcat tcttgagccc tggaatcttc
   123181 gttccggaat ggtgggcaac attctggccg tatcgtcggg gattgcctgg gcgggcagtg
   123241 ctgtcctggc aaaaatcata tggagaagat accccctgga gttgatatcc ctgtcggcgt
   123301 ggcagatgct cctcggttcc ctgcccctga tcatcatggc cataacgatc cccggcccgc
   123361 cggtccaatg gactccttac ttcatcgtcg gactctcctt cagttccgtg atcagccagg
   123421 ctttggcctt gatactctgg ttttacctgt tgaaagcgct gcccgccggt atggccagca
   123481 tgggcaccct ggccactccc atcatcggta tgatcgccgc cgctatcgaa cttggggaaa
   123541 ggccgtctct ctttgaagga ataggcattc ttctgatcct tgctggtctg atcatcctgg
   123601 ccctgcaggg gttgatccat gcgcgggaat taagggcgat aatcgccggt caaaaataaa
   123661 tcatattgtc aaggaggtag ggttatggaa agaaatcttc tcgataacat tgaagaaata
   123721 ctcttgatgg gaccggggcc atcctgtgtc ccccccgctg tttaccgtgc cttggcccgc
   123781 ccgacgctgg gacacctgga cccgtatttc ctgacgatca tggatgagct gaaaatcctg
   123841 ttgcaacaga tcatgaacac agcaaacaac cttaccgtcc ccgtgtcggg aaccggctcc
   123901 gccggcatgg aggcttgctt tgtcaacctc gtggagccgg gcgaccgggt gcttatactg
   123961 atcaatgggg tgttcggcat gcggatgcgg gaggtcgcca gtcgcctggg tgcggatgtc
   124021 gaggtcatgg agttcccctg gggaacgccg gtgaacccgt ccatggtagg ggaaaaactg
   124081 tgggaaggaa attaccggct cctcgccgtg gtccatgcgg aaacttccac cggggtggcc
   124141 aatccggtag cggagatcgg cgccctcccc cggggcgatg attgcctgta ccttgtcgat
   124201 gccgtgacca gcctgggcgg catggaggtc agaatggatg catgggggat cgatgccctg
   124261 tacagcggga cccaaaagtg cctctcatgc ccccccggtc tcgctcctct gtccttttcc
   124321 ccgcgggcgg tggctaaact gaacggtcgc cggcgcaagg tccccaactg gtatctggac
   124381 ctctccatga tcatcgacta ctgggggaag aaccgggttt atcaccacac cgccccggtc
   124441 aacatgctct atgccctgta ccaggcctta cagaccatct ttgccgaggg tctcgacgcg
   124501 gttttcaccc gccaccgacg cactcatgca atgcttgtcc ggggcttcgg ggagttgggg
   124561 ttgaaaatgg tcgttgagga acccttccgc ctgccgatgc tcaatacggt ctattgcccg
   124621 gcagggatcg acgaggctgc ggtacggaga gctctccgca gcgagcaccg gattgaaatc
   124681 ggcggcggtc tcggacccct gacgggaaag atctggcgca tcggcttgat gggccatacc
   124741 gcagccgaag agaacgtccg acgctttctc gacgcgctaa gaaccgtcat ttagaccttc
   124801 aggaggctac ggcgacattc tccttgatga gcatcgttgc gggatccaga atcagcaccg
   124861 gatgcggtga cggtgttcgt tcgctcttga gatcccggat gtgcatctcg ttgccttcgg
   124921 tcaccaggag gagcgccacg tcgaaaaggt cgtcggcggg caggaggggt ttggggatcc
   124981 cgtcggcatc cgcctgcacc tggcggtgga cgtgcacggt aaaccaggcc cgcagtgaaa
   125041 gttcttcgat catggacttc atgtccgcaa gtgcggattg ggggaagtca tcgaagatca
   125101 gcccgtcaat gatgatcgta tccgggtaga atatgccctg ggccgtgagg tcgttcagcc
   125161 tttcccggag cctggggacg ctgaacccct cgaccttgaa ggtcatgatg aaccgaagcg
   125221 gcaggatctc ttcccaaagc gtccggatct ggccaacctg atactggacg gcaaggtggc
   125281 ggaacaactc ccggtaccag agatcgacct tgttgacggg gtcatggagg ccgacatgaa
   125341 gcacccgttt gccccgcagc atcgtgttga gggcaaactg aaccagcagc gccgtctttc
   125401 ccactccggc ccgggcgagg acggcgccga atccccccgc cgggatgatg tcgtcgtcct
   125461 cataccccat ctggcgcagg gggttcctta aaataagatc ctttttcaac atcgtcatgc
   125521 tcctgtcctt caaatcgata atatccacca tgacctgcct gcctaagcag cgttcttctt
   125581 ccgggccgcg atctcttcgg cgatcttttc cgctattgaa ttaggaactt ggcggtagag
   125641 ggaaaactcc atggtaaact gcgccttgcc ctgcgtagcg gaacgcagga ccgtggaaaa
   125701 accgaacatc tcggcgagag gaacctgggc ttccacaatg cacatcacgc cttcgtcctg
   125761 ggcgccgaca atcattcccc gccgctgatt gaggagggcc atgaccgagc cctgaaattc
   125821 cgtgggtgat tcgataacca ccttcatgat cggctcatgg accaccggcc tggccctaag
   125881 ataggcgtcg cgaaaagcgc cccgggcggc ggcctgaaag gccatgtcag aggaatcgac
   125941 ggcatgatag gccccgtcgt tgatcaccac cctgatgccg gtaatgggaa attcgagctt
   126001 gggacccttc tccagggact ggcggaagcc cttttcacag gccgggatgt attgggtcgg
   126061 gatagcgccg ccgacaatat cgttttcgaa aatgaaatca ccctccgagg tcggctccat
   126121 gtagccggcg atccggccgt actgcccggc gccaccggtc tgtttcttgt ggatgtagtt
   126181 gaaatccgcc cgctgggtaa tcgtttcgcg ataagcgacc cggggattgc cggtagtgac
   126241 ctcggcgccg tattcccttc gcatgcgctc cacgtacaca tctagatgca actcccccat
   126301 gccggcaatg atcgtctcgt tggtttccct gtccacgtgg catttgaagg tggggtcttc
   126361 tttggtgaac cgggacaggg ccttggacat attgatctgg gccttattat ccttgggtgc
   126421 gatggccagg gaaatgaccg gtgcgggtac atacatggaa gtcatggcca agttcaggcc
   126481 cggggagaca aaagtgtcgc cggaggcgca ctcgacgccg aacagggcac cgatatatcc
   126541 cgccggaatg gtgtcgatgt cttccatttg atcggcatgc atccgggcga gacgaccgac
   126601 cttgatcttt ttccccgagc gaacgttcac gatggtcgtt cccttggcga ttgttccctg
   126661 atagacccgg atgtaggtca actgtccgaa agggccgtct tcaagcttga acgccagggc
   126721 gacgatgggt tcatccgggg aatttttcag gattaccggc gcttcgtttt cgtcaagatt
   126781 caaggcggtg ttttccacct ccgccgggca gggcaataaa ctcgtcacgg tatcgaggag
   126841 tttctgcacc cccttgttct tgtaagccga accgatgacc acgggggtta tccgcctttt
   126901 cagcgtgccg aagcggaggg cggtaaagat catcgccgtc gttatctccc gctcttcgag
   126961 gatcgcctcg gtcaagtcat cggaatatat ggaagcctga tctatcatct cctcccggag
   127021 gcgcaacgcc tcagggagga ggacggcggg aatctcctca tagcgaagtt tttctccgtg
   127081 ggggccatcg aaatatacgg cccgcatctc gataagatcg acgacgccgt gaaaatttga
   127141 ttcgagaccg atgggcatct ggagcaatac agcgttgtgg cccagtttgc cccgtaattg
   127201 ctcgaccaca cgtttgggat tcgccccgct gcggtcgcat ttattgataa aggcgatgca
   127261 ggggacgcgg tagcgtttca tctgctgatc cacagttatg gactgggact ggaccccgcc
   127321 tacggaacat aaaaccagca cggcgccgtc caggacccgg agcgaccttt ccacctcgat
   127381 ggtgaaatcc acatgaccgg gggtgtcgat gatgttgatt tcgtggtcgt tccattcaca
   127441 ataggtagcc gccgaagcaa tagttattcc ccgttccttt tccagttcca tggaatccat
   127501 ggtagcgcct acaccgtcct tgccctttac ctcgtgaatg gcgtggatgc gtttggtgta
   127561 aaagaggatc cgctccgtga gcgtggtctt gccggagtcg atgtgggcgc tgatgccgat
   127621 atttcttatt tttgccaagt ccctcttcat aagtattttc ctataccttg tatgcgctga
   127681 aaaaacgagt cttttagtga agcacaacag gtttgtcaag cattatttac aaaatccgtc
   127741 ttcaccaaga agatgaacga aagaaaacag gaagatcagg aagtgcttga cagccgtcaa
   127801 aaaagaacgt ttgatatgcc ccatcactta ttacaaggtc gggcatgaat tttaccgtac
   127861 atacctcatc ggattatcat cgacgggaac tgacgagcct gggctgggag acgaccctct
   127921 gcaacaccat tgccgaccct catggtccct gccgcacggt cctcggcatc gacgccgctt
   127981 atggggagtt gctgggcgat tatctccggc gctatgtccc cttcccggac atctcccatc
   128041 ttgtggaggt cggcggcggc tacggctggc tcatgaggga tctgcttcgc cggcatcact
   128101 ttcctaaggt gacgatgatc gatatctgcc ctaccctcct ggccgttcaa aaagaaacgc
   128161 tggggagaat tcgtggtgtc atcgatttcc gggaggagga ctttctggat accccgcacg
   128221 agatgctccg cggggccgat ctagtgatcc tgaacgaaaa tgtgggcgat tacccaacca
   128281 ttacggacct taccagggaa agcctggaga atcgcgaggc agcgccggcg ccggaactgc
   128341 gggcagtgag cgcgttgttc catagatacg ggttgccggt tccggatcaa gcgagattcc
   128401 atgtgaacat cggggccctt ttgacactgg aaaagctctg ccgcgccgaa gtcccctata
   128461 tatttctcag cgaacacagt tgcgaggcct cggtctccat cccctaccgg gacctgattc
   128521 acgtccccgc ctcaggcaat cccgaatgca tcgtcctgaa aggtcacgag gaattcacca
   128581 tccgcttttc ccatctggaa aagctggccg aaagttacgg ctatcgaacg atccgggggc
   128641 ccatcgccga tttcatccct ttccacgtca ccccgcgggt ccgggcgatc ctcaaggccc
   128701 cctctccctg gcgggacgaa gacgaaatcg tccgttattt tctcgaggat ctcttcaaat
   128761 acgaatatct gctcctggct aaggagaccg agaccgagca tgcccaagac ggcggcgatc
   128821 tcacctgccg gcgttgcggt aagtgctgcc tggccgactt cgcggcgtat gtgaaggatg
   128881 aagacctgga aagatggcgg cgcgaaggga gggaggatat cctggcgatc atagcgcgtg
   128941 agcatgccgt ctgggcgggc gaccatctca tttccgccgc cgacggccgc tacctccacg
   129001 ggtgcccttt cctgacttgg gaaggcgtcc acacgacctg tgccatttac gagacccggc
   129061 cggcggtatg ccgtaacttt aagcccgcct ccgtcgagat ctgcccgttg tggcgaccct
   129121 gatagtatag aacctatttt tattgaaata tttttagatt tgtattaaaa aatcgccgcg
   129181 gcaacaagat cagtaaacaa cgaaaaggag gcgtcatgct gagacatatc gtcttgatga
   129241 agttcaagga gggcgttgga gagcgccaga ttgaagaaat gtccgcggcc ctgcgttccc
   129301 tgccgccaaa aatatccgtc atcaagggtt tcgaattcgg tcccgatatc ctcagaaccg
   129361 atcgttccta tgatttcgcc ctggtggcca gttttgaaaa cgtggatggt ttaaaggcat
   129421 atcagcttca ccccgatcac gtcccggtgc tgcaactggt gagaagtctc tgcgatgccc
   129481 tcatcgtggc agattttacc tggccgtaaa ctatttccct tggaggtaat cagtcatgcc
   129541 cacccctgta aaactatttt ccctgagtac ttgcagccac tgcaaggcga cgaagaagtt
   129601 cctcaccgat tgcaacatcg cctatgattt caccgatgtg gatcgcttga gcggtgatga
   129661 aagacaactg gccatcgacg aggtaatgaa gtacaacccc catcgctcct ttccgacaat
   129721 catcattggc gacaggataa ttatcggctt caacgaaaag gctatccggg aggccctggg
   129781 gatctgatgg acgccgcgcg actttacgaa acgctgaaaa agattcagga gcccaaggga
   129841 tttcttttca accgcgacaa gaactgggtc atggaaatcc tgaatgatct cttggtaaac
   129901 aaggaacgat acggatacat gacctgcccc tgccggctgg cttcgggcaa tcgggaacag
   129961 gaccgggaca tcgtctgccc ctgcgcctat cgcgaggcgg atgtaaagga atacggcagt
   130021 tgctattgca gtctctacgt ctcccgggaa tggaatgaag agcggattga acacctctac
   130081 gttccagaac gacgtcctcc ggaaaagatg ggcatctgac catgagcggc aacaagccca
   130141 tcctcgtctc gggtaatctc cccgcggcgg tgctggactt cctcagggaa gatttccagg
   130201 tcgagtacca tgccgaggac cggcccatgc cccggcgcaa actcctgcgc ttcctggacg
   130261 ataaggtggg cctgctggcc atgatcaccg accacatcga tgaggaactg tttgaccacg
   130321 ccccgcgact ggccatggta gccaacatgg ccgtgggtta caacaacatc gatgttgtgg
   130381 cggcgacccg gcggggcatc ctcgtctcca acacccccgg ggttcttacg gaaaccacgg
   130441 cggatctcgc cttcaccctc ctcttggcag cggcccgaag ggtcgtcgaa ggcgaccgca
   130501 tggtccggtc aggacgtttc aagttctggg cgccgtttca ctttctggga cgggaggtaa
   130561 ccggcaagac cctggggatc gtggggatgg gcagaatcgg tcaggccgtg gcgcgccggg
   130621 cccggggctt caatatgccg gtgttctatc acagccggac accactccgg aaatccgtgg
   130681 aaaagaatct ccgggccact tttctccccc tgacttccct tttgaaggag gccgatttcg
   130741 tatccctcca cgtccccctc tccgaggaaa caacgcatct catcggcagg gacgaactgg
   130801 ccctgatgaa gccgaccgct tttctcatca atacctcccg cggtcccgtg gtggatgagc
   130861 aggccctcct tagcgccctt cgggaaggaa tcatcgccgg cgccggcatt gacgtctatg
   130921 agcacgaacc ggtcattgcc accggtctgg aaaagatcga ccaggccgtc ctcctccccc
   130981 atgtgggtag cgccactttg gagacaagga cccggatggc ctggatggcg gcggaaaacc
   131041 tccggatggg cctccggggg gagagaccgc cgaatctggt caacccgcaa ctcatgaaga
   131101 agaggaaaag aggcgtataa tgcccttcag tttttatccc ctggacatcc ccgaggtcat
   131161 cggtatcgat taccggcgtt ttgccgacga ccggggttat tttgccgaaa cttacaagcg
   131221 atccgatttc gtcgctgcgg gcatcgcgga agatttcgtt caggacaatt attcctattc
   131281 gcgccggggc gccgtccggg ggctgcacta tcaaaaaaac cccatggcgc agggtaaact
   131341 ggtcatggtg gcggcgggaa cgatattcga tgtcgccgtg gacatccgtc gcggctcgcc
   131401 cagcttcggc cactgggtcg gcagggagct ctcgactcaa aacggcgcca tgctctatat
   131461 cccgccggga tttgcccacg gcttctccgt tttaagcaac gaggccgtcg tcgtctataa
   131521 ggtgacgcgg gaatatgccg cggagctgga tcgggggata atcttcagcg acccggaact
   131581 ggggattccc tggtcggttc cggaccccct ggtttctccc aaggatgccg cctggccgcc
   131641 cttgcgaaag gccgacatcg attacaatta cgggcgctga agcccttatc acgacgaatg
   131701 gtgggttgga ccgtggaaaa tcgcattcag ggcaaaagga tactcatcac cggggccagc
   131761 gccggcatcg gcaaggcctg cgcggaacgt ttcgccgccg ccggagcgaa tctcctgctg
   131821 atcgcaaggc gtttgggccg gatcaaggaa ttgcaggccg aactctccag agaaaattcc
   131881 gtgggcatcg actgcttttc cctcgacgtc cgggatcgcg aggcagtgcg aaaatttgcg
   131941 gcggaactca cggacaggaa actgacgccg gatatcctca tcaacaacgc cgggctggcc
   132001 tccgggctga attcactcca ggaaggcgac tgggatgact gggaccggat gatcgacacg
   132061 aacatcaagg ggcttctcaa cgtgtcccgt tgcctcatcc ccctcatgat tgcagccggc
   132121 cggggccata tcgtcaatgt gggcagcctc gccggctatc aggtgtaccc taaaggaaac
   132181 gtttataacg ccactaaatt tgccgtccgc gccctctccg agggaatgaa catcgatctg
   132241 gccgggacgg ggatccgggt atccatgatc tcccccgggg ccgtaaatac ggaattttcc
   132301 gaagtgcgtt tccacggcga ccgggaagca gccgcccggg tttatgaggg ttatactcct
   132361 ctttatgccg aagatgtggc cgaggccgta tatttcatcg tcaatgcccc gccgcatgtc
   132421 aatgtccaga acatgctcat cacccccacg gcccagcgga acgtctattg tgtggaccga
   132481 gccgacaaat cctgaaagat cgggatcatc ggcgcgggag cgccaagcgg cggctactat
   132541 tcatccgcgg gcggacggcc gtgcaacggt tcgcatcgtg agatctttga aaaatggcat
   132601 cgccacggct cagaaatctt tttcgattct gaatgggtaa cgtataaata ttatttataa
   132661 ccaggtgtta agttcaatca agaaaacggc gaaaaacatt tgattcgccg cttggaacca
   132721 ttttcccgca gttatgcaaa gcccccatcg tcatctcagc ggcacaattc cagcaccgcg
   132781 ccgatctcct gacgggagta agccttcaga ccccagacct gggccagttc cgcggcattt
   132841 tcggcgatac gatcgatatc accggcggga atccccgcct cggccaggga tacgggggag
   132901 ccgatggcgg caaaccactt ccttaacaca ttgatgcccg cccgggcggc ttcatcgttg
   132961 tcggccgtgt tgatgccgaa gacctccctg gccagacggg cgaaacgccc cgccttctcc
   133021 cggacctggc ggctcatcca gccgggcagg acgatggaga gcccggcgcc atgggcgaca
   133081 tcgtaaaggg cgctcaggga atgctcgatc atatggaccg gcagggcaac ctgccccatg
   133141 cccgccgtcg tcaggccgtt gaaggccagg gtggctgccc acatcatctc ggctcgggcg
   133201 ttgtaatcat ccggcgccac gagaatcttt tccgtggctt ccatgatggt cttcatcagc
   133261 ccctccacga ggcggtcctg caaccggctg aaagccggtt ccgtattgtt gaaataaccc
   133321 tcgagcatat gggtgatggc atcaacggcg ctgtaggcgc tgtatttttt gtccaggctg
   133381 aacagaacgg tcggatcgag gacggatacc ttaggctgca catggaggga gcggatgctg
   133441 tacttccggc gtccctcttc cttggtgatc accgccgcgg cattcatctc cgaggcgctg
   133501 gcggacaccg tcaccacggt gagaaccgga agcgcttcct ggatggtctg tcggtaaaca
   133561 aaaaaatccc agacgtcatg gtcggtacgg gctccggcgg caatggtctt ggcggtgtcg
   133621 atagcgctgc cgccacccac ggccagcacc gcctcgatcc cctcccgctt cgcgatttca
   133681 atcccccgga cggcatggga gagcaccgga ttggagcgga ctccagaaaa ttccaccacg
   133741 gtgagaccat tttccttaag cgacgccatg acctgctcat agacgccgtt tttcttgatg
   133801 cttccctgcc cgtaaaccag aaggatcttc cctccgaacc tcgccgcctc ggcgccgatg
   133861 cgtctgattt gcccctggcc gaaaatgatc ttcgttggat tctcgaaaat aaaattttgc
   133921 atcttgattt tcctttccag aagatatttt tttgaatata ggaagatact ctggtaatgt
   133981 caatatgtgg cgaagctccg gcggcttccg ttcgatcgtc gccatccggc atttgtgtaa
   134041 accaaggcgg ttttttgact ttaaacccaa agaggaggga aagtatgaag gccaagaatc
   134101 acctggatgc attgttcaaa ccccgcagcg tcgccctgat cggcgcctcc ggcaccccgg
   134161 gaaagttagg ttgcgacatc ctctacaacc tcatccacgc cggtttccaa ggaccgatct
   134221 atcccatcaa ccccaaggcg gatgaattgc tgggcctgaa ggcctatccg gacatcgagg
   134281 cgacaccggc gcccgccgac ctggctgtga tcgtgattcc ttcccggatg gtcaccggca
   134341 cgatagaaaa gtgcggccgc agcggcgtcc gggcggcggt gatcgttacc ggcggctttg
   134401 ccgaggccgg cgtggaaggg gaaaaactcc agcaggagct ggcagccgcc gccgcccggt
   134461 atggcatccg ggtggtggga ccaaactgtc aggggatcaa ttatccccat cacaacctct
   134521 gcgcctcctg gcccctgctg accaacagag ggggcatggc cttcatctcc cagagcggca
   134581 ctgtaggcgc cgccctgatg gactgggcga gcgaggaaaa tctcggcgtc agcgtcttcg
   134641 tcagcctggg aaaccgcgcc gacgtcgatg agtcggactg catccgttat ttcgacaagg
   134701 attcccatac ccgggtgatc gccatctaca tggaaggggt aaagcgaccc caatactttc
   134761 tggaatccct ggccaaggcg aaaaagcccg tggtcatcct caaggccggc cggacggccc
   134821 ggggcaaagt cgccgcggaa agccacacga aatccctggc gggaagcgac gagatctata
   134881 acgccatctt cagtaagtac cgggttcacc gggcggacaa tctcgaggag ctgtacgatt
   134941 ttgcgaaggc cctggcctat atgaaaaaac ccaaaggccg acggctcctg aacatatcca
   135001 gctccggcgg ggcggcgatt ctcgccatcg atgaggcgga gaagctggga tttaaaaatc
   135061 ccgctcccgg caagaacttg aaggaaaaac tccgctcctt cctgcctgcc cattgcgggg
   135121 taagcaatcc catcgacctg accggtgacg ccatctccga tcccagcctt tattcccggg
   135181 ttatcgacgc ggccaaacag gatttcgaca ctagcgtggt catttttggc gatcccatca
   135241 agggggcggc ggatatcgta tcgggcgaag gcgaattgat cgtcttctgc ggcggcgccg
   135301 acgtggaacg ggaggaatcc cggcgcatgc atgccaaggg catcccggtc tatcccacgc
   135361 cggagcgggg catccgggcc ctccatcagc ttgtatgctt cgatcctccc cggagtgtca
   135421 aaaagaagaa agatgatgca aaaccggagc accaggggcg gcagcctctt tccctggata
   135481 cggccgtcgc gctcatggaa aaatacggcc tgccgttcgt tgcgacgcgt ccggccgcta
   135541 cggccgcggc cgccgccacc ctagccaagg ctatgggcgg gcccgtggcg ctgaaaatct
   135601 cctcccccga tgccgcccac aagaccgatg tcggcggggt acggctaggt gtgaccggtg
   135661 ccgccgcggt ccgcaaggcc tacgaagaaa tccaggccaa tgtccggacc gccctgccgg
   135721 aggcccgcat cgacggcatc actgtctcgc ccatggcccc cccgggagga ttggagacca
   135781 tcgtcggcgt catacgggat ccccagtacg gccatgccct gatgttcggg ctgggaggta
   135841 tcttcacgga aatctatcgc gatgtccgtt tttgcctgct tccggcaacc gaacgggaat
   135901 tgctcgcgat gattcgcggc atcaagggat accccctcct ggccggggtg cgggggcaaa
   135961 aaccccggga cgagcaggcg ctggttgccc tgatgcggtc cctggcccgc atggtccagg
   136021 atcatgggga tatcgaccag atcgatctca atccggttct ggtctatgaa cagggtgcct
   136081 tggccgtgga tttccgtatc tatggttcaa tatgatcgca gcgacggcat gcagggggaa
   136141 ggaaccgtgt atctcttccc tcttcatgcc gtaaaaagga gccttcatga agatagcaga
   136201 cggtctgtat agttacgagt ggaccaatta ttacgaaaac aattgcaaca gtttctttat
   136261 cggcggcggc gtcggcgccg tcatcgaccc gggtttgaag agctacgttc ccgatctctt
   136321 gcagcggatg gccgccgacg gcatcgacgg aaaggacata cggtatgtca tcaacaccca
   136381 ttcccatccg gatcacttcg agggatcgga atacttccac gggcgggaca tctccatcgg
   136441 ccttcacgaa gatgagctct cctttctggc cggcgtcgga ggggaattgt acggattgtt
   136501 cggactgccg cggccaagtg tcgccgtaga tttagcgttg tcggaaggaa cggcggaatt
   136561 tggcggggaa gaatttcaga tattccatgt cccgggacat tcgcccggat cgatcgccct
   136621 ctactggcca agggcgaagg ccctgtttcc cggcgatgtc atcttcaatc agaatgtagg
   136681 ccggacggac tttcccggcg gcagcggcgc gctcttaaag cagagcatcc ggtccctcag
   136741 cggcctggag gtcgaatggc tcctgccggg ccatatggaa ataattggcg ggacggaaaa
   136801 cgtccaaaga aatttccaga ccgtcattcg ccacatcttc ccctatatct gagcgttcgg
   136861 gaacccatgc ttacctccag aagaacggtt ttcttcattt ccgacagcac ggccatcacc
   136921 gcggaggccc tggggcagag tctgcttacc cagttcgacc atgtcctctt cgagcaggtc
   136981 accctgccct ttatcgacac ccaggccaag gcggagcggg tggtcaacca gatcaatatg
   137041 gcggcaagac aatcaggcct cccgccgctg gtcttcagca ccctgatcaa tcacgatatc
   137101 cgcgccaccg taatgaaaag ccaaggcagc tttttcgatc ttttcgagac cttcatcggc
   137161 cggctggagc gggaattgag catggaatca tcccatacca tcggccgcta ccacagcttc
   137221 atcagcaaca gcatctatga cgtgcgcatc gacgccgtca attatgccct ggggaacgac
   137281 gacgggatgt ccacgaagaa ctacgacaag gccgacatca tcatcgtcgg agtgtcgcga
   137341 acgggtaaaa cccccacctg cctctacctc ggcatgcagt tcggaatcta cgccgccaac
   137401 tatcccctta cggatgagga cctctttggt agatacgacg aacttcccgg cgtcctcatc
   137461 cacttccgga agaaactcta cgggctgacc atcaaccccg aacgactgca ccggattcgc
   137521 acggagagac ttcccgccag ccgttattcc tccctggagc aatgccggtc ggagattgcg
   137581 gcggcagaat ctcttttcct ttccgagcac atcccccata tcaacatcac gagtgcctcc
   137641 gtggaggaaa tcgccgccat gatccttcat gaatgtggtc tgcaacgacg cgtattatga
   137701 ttttttactt gcatttgctt cttgattgcc atagtaaatt tcggatttga tcggaggctc
   137761 atccgggaaa tgacgctgcg ccagaagata tatcttgttg ttgcccttac attttttgcc
   137821 ttgacgatgg tcatggcgtt ttgctcgtat acgattctca tcaagggttt tgaagacatc
   137881 gagagagagg atgtgcgcag caatctgtac ggcgccttgg cgctgctgga ccaggaactc
   137941 gcgtcaatgg acgccacggc ccgggactac tcatcctggg acgatatgta tgattacatc
   138001 ggaacccgca gggaatcatt catcgcctcc aattattccc agtccggcat caccaatctg
   138061 aaactcaatt ttctgattat caacgatctg caagggaaca tcgtctacag caaaggcttc
   138121 aatccggaaa ccggccacac cgtgccgatt ttaaggagca tcctcgctca ccgcaatctc
   138181 atccacaaac atgtcttctc gggagacgaa atcaaacatc tgtcgggaat catttccctt
   138241 ccggagggct tgacccttgt gatcgcccgg ccgatcttga aaacgtccgg caagggaact
   138301 cctcggggta catacattgt cggccggttt ctcgacgcca gggagatcga ccaaatgggg
   138361 gccccgctga aatcggaagt gtcgatttac catgaggggc aaaaggatct gccggcggag
   138421 atcgcctccg tccggaaagc attgtccccg catcaccggg agctgataaa ggccatcaac
   138481 ggccatacta ttgcgggata cgtcttgatc aaggatgcct tcggaaaata ctcactgata
   138541 ttgaagacca tagacaagcg agtcattttc atacagggga tgaagaccat tgcctatgtg
   138601 atcgttttcg tgctccttac ggccctggtc tttgcctcgg tcatggtggc gcttttggaa
   138661 aaatccgtcc tccgccggat gttgcatatc agccacgaca tccgggccat cgaagcggac
   138721 ggtgacatga gccgccgcgt ccatcttggg ggcacggatg aaatcgccaa cctcggcaac
   138781 gacataaaca atatgctcgg caagctggag gaatccgagc agaggatccg tggcgaagcg
   138841 gaaagatacc gtgccgtgat cgaggatcag acggaattca tctgtagatt cggcgcggac
   138901 ggggtactga gcttcgtcaa cgatgcctac tatcgctttt tcagccatcg gcgccaggag
   138961 atgatcggcc ggcatttcct ctcctttgtc ccggacgagg atcgacgcct gatctatgaa
   139021 ctcttgggca accttactcc ctcctccgcc acagcgacct acgatagccg ggtcatcttc
   139081 ccggagggtc aggagttctg gcaacactgg acctatcggg ccatatatga cgcttcgggc
   139141 tgtttgcggg agtaccaggc ggtgggccgg gacatcaccg atcggaagca tgcggaggaa
   139201 aagctatccc gcctcaacag gcaactcgag gaagccaacg cccaactcgc ccaggcttac
   139261 gccaacatga aacaaaacct ggacaccttg cggaaacacc tgttcaagga ggaatttgcc
   139321 ttcctcgttg atcgggaagg acagatattg gggattaccg aacgggtgct ggagtatatg
   139381 aagctctccc gcaaccagat gatcgaggct aacgtgaccg attacctggc cacccatgac
   139441 cgcggaaaat tcatcgaggc ccttcgtgac gcctggatcg gcatcaccag gcaggcgaat
   139501 ttcaaactga ttcgtcctca ggatgaaccg tcgtcgcagc atttcgaaat caaattcgcc
   139561 cgcctgacct tagaagggaa aaggctgctc ctcgtcaccc ttagatgatt aataaatgca
   139621 accttgaata ttttaccgtt tttgttataa ggagaagcta aattattgac gatccttttt
   139681 atttgcacgg ccaatatcgt cagaagttac atggcggagc ggattctgaa aggcatgctg
   139741 gcgaagatga agcgtcggga tgtcatcgtc acctccgcgg gcatgttgga tatgcacggc
   139801 gcccccggcg atcccaccgc ggcgaaaatc ctcgccgagc ataactttga cggttacgga
   139861 catatatcga gaccgtattc ggaggcgttg ctgtctcagg cggatatgat tgtcgtcatg
   139921 gaaaaggcgc aggaggagcg attgacggcg gatcatcccc ggtatgggga aagactgcgg
   139981 ctgctgaaaa cattttcccg ggaatacgac gggagcgccg gagacatcaa ggatccctac
   140041 cggctttccc cttaccatta tcgactctgc
//
LOCUS       DAOSMF010000002        97247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_257_length_97247_cov_21.064686, whole genome shotgun sequence.
ACCESSION   DAOSMF010000002 DAOSMF010000000
VERSION     DAOSMF010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 97247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 97247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..97247
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_257_length_97247_cov_21.064686"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            329..1327
                     /locus_tag="PLT63_00635"
     CDS             329..1327
                     /locus_tag="PLT63_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent 4,6-dehydratase LegB"
                     /protein_id="HOD97255.1"
                     /translation="MDLTKLSILVTGADGFIGSHLVEALLSRGCRVRAFVFYNSFNSW
                     GWLEALPKELRDGLDVFAGDIRDPHGVAKAMAGIDVVFHLAALIGIPFSYHSPDSYVD
                     TNIKGTLNVLQAARESGCDRVLITSTSEVYGTAQYVPIDERHPCQGQSPYAATKIGAD
                     RMAESFYRSFDTPVTIVRPFNTYGPRQSARAVIPTIISQLLGGVRKIKLGNLSPTRDF
                     NYVLDTVRGFIAVAETPAAIGEEINIASQQEISIGRIAREIIDQIDPAAVIECEDMRI
                     RPEKSEVERLLGSNEKIRSLTGWEPAYDLKEGLRETIAWFQDGGNLRRYKWDIYNV"
     gene            1320..2462
                     /locus_tag="PLT63_00640"
     CDS             1320..2462
                     /locus_tag="PLT63_00640"
                     /inference="COORDINATES: protein motif:HMM:TIGR04181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LegC family aminotransferase"
                     /protein_id="HOD97256.1"
                     /translation="MFEKTVAFIKSLYPGEVPVPLHAPRFGGNEKKYLLDCIDTTYVS
                     YVGEYVGRFEAKIREYTGAAHAVAVSSGTAALHTALMLAGVSPGDEVITQPLTFVATA
                     NAIAYCGARPVFVDVEQTTLGLDPDKLADFLAAHARPDPHGGCRDRTTGGRIAACVPV
                     HTFGHPARIDRILEICGHYGLPVVEDAAEALGSFYRGRHAGTFGEVGILSFNGNKPVT
                     TGGGGMIITGSDTLAARAKHLTTTAKRQHTWEFFHDEVGYNYRLPNINAAVGCAQMER
                     FAGVRENKRVTAELYRRFFAASGMEFVVEPAHAQSNYWLNALVLKDREERDAFLAFSR
                     GKGIQTRPVWSLVSDLPMYRHCRRDGLETARRLEGRLANIPSSVRI"
     gene            2459..3049
                     /locus_tag="PLT63_00645"
     CDS             2459..3049
                     /locus_tag="PLT63_00645"
                     /inference="COORDINATES: protein motif:HMM:TIGR03570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NeuD/PglB/VioB family sugar acetyltransferase"
                     /protein_id="HOD97257.1"
                     /translation="MKKQILLIGGGGHCRSCIDVIEREDRFVVAGIVDVLEKRNQTVL
                     GYPVIGSDGDLPALIKLYPQVLITLGHIRSPRRRMELFGELEDMGADFPVIRSPLAYV
                     SPHARVGAGTIVMHHALVNAGARVGKNCIVNTRALIEHDAVIDDHCHIATGAVVNGGV
                     TMGSGTFYGSCAVSKEYTTIPPGSFIKAGGIYPEKR"
     gene            3046..4104
                     /gene="neuB"
                     /locus_tag="PLT63_00650"
     CDS             3046..4104
                     /gene="neuB"
                     /locus_tag="PLT63_00650"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: protein motif:HMM:TIGR03569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HOD97258.1"
                     /translation="MNRTFIIAEAGVNHNGVLATAREIIDAAVAAGADAVKFQTFQAE
                     KLVTAAAAKAAYQLENTGGDESQFEMLRKLELSRDDHRALWRYAQEKDIIFMSTPFDE
                     ESADLLAELGMEIFKIGSGELTNRPLLEHIARKGRPMVVSTGMSYLYEVARAVQWLEE
                     ARAAAKDPPGPSRRTFPYPLVLLHCVTNYPAAPAAVNLRAMQTMKEAFHLPVGYSDHT
                     PGMEMAVAAVALGAVVIEKHLTLSREMAGPDHRASLEPAEFAALVRAVRNVEAGLGDG
                     IKKPAPSELPYRDIVRRSLVLTRDIRAGEKIALPDLSLKRPGKGIAGEFRAIVLGMRA
                     VRDLQKDAHLSWEDLKNA"
     gene            4097..5275
                     /gene="neuC"
                     /locus_tag="PLT63_00655"
     CDS             4097..5275
                     /gene="neuC"
                     /locus_tag="PLT63_00655"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: protein motif:HMM:NF014411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HOD97259.1"
                     /translation="MRRIAVVTGSRAEYGLLYWLMKEIQEDVDLELQLIVTGMHLAPE
                     FGMTVKVIERDGFPIAERVEMLLSADSDAAVAISMGLGLIGLAKAYQRLQPELIVLLG
                     DRFEIFAAAAAALPLAIPVAHIHGGEATEGAIDEYFRHAITKLSFLHFAATPRYRRRI
                     IQMGEAPDRVFCYGAPGLDNIGRLPLLTAAALREELGLTTARRNLGVVTYHPETRGVE
                     PAAEQINAVLTAVSGFDDIYWLFTLPNADQGGRELGGMIREYLERRPEVGTLFASLGQ
                     LYYLSLLKNSLVMVGNSSSGLIEAPSFRLPVVNIGARQEGRERAANVIDVPRCTVAAI
                     AAGIGSARSAAFAASIETMENPYGHGDASGKIKEALKTVPLDKQLLKKRFYEGADGTE
                     "
     gene            5262..6332
                     /locus_tag="PLT63_00660"
     CDS             5262..6332
                     /locus_tag="PLT63_00660"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOD97260.1"
                     /translation="MEQNKLQSLLVPPELTIKEAMQKLNESAQKILFVIDGRQRLVGT
                     VTDGDIRRAIIGGNGFSDPVAKIMYRKFAFLTRGAPALLAAAKELMIAREIEQLPVLD
                     EDGAVRDVILWTDILEKKGADVPVSRHENRVVIMAGGKGTRMDPFTRIFPKPLIPIGK
                     KTVVEMIMDQFHRWGFQHFILTLNYKKEYIKVFLQEAELPYGVDWIDEPEFLGTAGSL
                     SLLKDHLGDTFVVANCDSLLDLDVAEVLAWHRDQGAIMTVIGCYHEVKIHFGVLELGE
                     GRLRRILEKPSHDTIINTGIYVLEPRAIDYIPAGEEVHMDGLIGKMIAAGEKVSVYPI
                     HSGWLDIGQWDQYKKAIRDYDL"
     gene            6322..7329
                     /locus_tag="PLT63_00665"
     CDS             6322..7329
                     /locus_tag="PLT63_00665"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOD97261.1"
                     /translation="MTCEQPRRAAIIGLGNIGFRFDLQSRRRGTWSHAAAYTRSPETE
                     LVGAVELDGNKADLFRKHYPAVPVYDDIGRLMTACRPEIVSICTPTESHYPLLKELLV
                     YPLRGVFCEKPLAANAAEGLEMVRAAAAHGIVLAVNHTRRWDPGFLRVQELVRRGELG
                     AIRAVHAVYPGQVFNIGTHLMDVVCMITGRAPVCVSGVALPGGGDDPHLSGWLRLDDG
                     TVCTINALGRRELLVFELDFIGARGRVRISDNGESLEGAAFVHSDQYDGYEVLRRREL
                     PAPPERDRLVEAVTDMAMVIGGRKDKVNCTGADGLVALRLAAALAASARRHGRPVAVE
                     G"
     gene            7392..8762
                     /locus_tag="PLT63_00670"
     CDS             7392..8762
                     /locus_tag="PLT63_00670"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOD97262.1"
                     /translation="MSELALLGGTPVRRTPFRSSVVIDDREWEELRNVLKREEFSRFM
                     GSPTPDIDKLLVMPSAAAADLEGQYFSFLGGRTVRRFEAEFAARFGVTYAISVNAATS
                     GLAAALGAAGIGPGDEVITTCMSFNATAMSILLFNAVPVFVDVDARTFCLDPEAVERA
                     ITPRTKAILVVHLLGNAADMDALLAIAGRHGLKVVEDAAQAPGTTYRRRWVGTLGDMG
                     VFSFQETKNMMTGEGGMIVTNDPALAKKARLIRNHGEGIPDETWDDESLTNLVGLNFR
                     MTELTAALGRAQLAKLAENNRIRTENALLLRDRLPDLPGLTRPDIPPGTVPHVFPMLY
                     DEAATGVPRQKVLAALRAEGIPVGSGYLRLMYENPLFLRKIAYGKHGCPWSCHLYGRE
                     RRYLPGQCPVGEALLRRRFLWFYHIHRPNTAADMEEVAVAFRKVFMNLEDLRAATGDF
                     TIPYKW"
     gene            8767..9486
                     /locus_tag="PLT63_00675"
     CDS             8767..9486
                     /locus_tag="PLT63_00675"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HOD97263.1"
                     /translation="MENDLNIVGIIHARGGSKRIPLKNIKLLGEEPLIAYLIKAALAS
                     RHLRRVIVSTDHDEIRSISRRYGAEVPFTRPAHLAEDCPSEWVSRHAVEFIESQEGRP
                     VDIAVTMQPTTPFCTGADIDACIEVLLADKELKSSFTAKTVTERPEWMFRRRENQRAE
                     LLLAGAMKGERGIFQSLSELVIPNGAVYATRRDTLFDEGVIIADKTGVSVMPAERSVD
                     IDEPIDFLFAEFLLTKGVIHE"
     gene            9479..9664
                     /locus_tag="PLT63_00680"
     CDS             9479..9664
                     /locus_tag="PLT63_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97264.1"
                     /translation="MSKLLKGNYEIDRKEDNETAAAANNDFTKVFESKALEKLGGSIA
                     FVVKADQFVWDGDDDVK"
     gene            9651..10688
                     /locus_tag="PLT63_00685"
     CDS             9651..10688
                     /locus_tag="PLT63_00685"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HOD97265.1"
                     /translation="MTLSEEQFRLLSEATLRKTAARGLEHARANLPYLQKTIRDLQAE
                     LAPAPRRPALVISAGPSLHKRNSIGKIKDRGFEGPIVCADGALGHCLRNGLIPEYVLT
                     VDPDPHRIIRWFGDPRLTERPADDYFRRQDLDPALHKDEERKNEEMIALVNRYASRIK
                     LIIATSSSPEIAQRCRAAGMELYWWNPLYDDYDRPDSVSRTVFRLNKVSCMVTGGNVG
                     TSAWVFAQSILRSAAVAVVGMDCSYPPGTKPENTQYYEHLKEIFPDDPARGLVNIYNP
                     HLRETWLTDPGYYWYAQIFLKLVQMAPAPTFNCTEGGILFGPGIEFRPLVEILSLFHD
                     GRDYSAATRTT"
     gene            10691..12184
                     /locus_tag="PLT63_00690"
     CDS             10691..12184
                     /locus_tag="PLT63_00690"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOD97266.1"
                     /translation="MAKILFINPVIRENEKPAHVPFGMAQLVAICVRAGHQVQVFDAN
                     AWRPTDDEILAVLKADEWRVVAIGGLVTTYGFVKKVLRYVKKACPQALTVAGGGFLTP
                     IPHEVMALLPEIDLGVIGEGYVTLPDVLARVDAAEEDWHEIPGLIYRDRDGVCQLTAG
                     RPLLEEVDTLPFPAWDLFPLDIYFRNSGLLLSEEAMLAKRHLGVIASYGCPYRCKYCF
                     HLGLSGEIEIVEGPAGRRLCLTSKRKVRAHSPEYVVAMVAEAKKRFGIDFVSFLDENF
                     AVLAKRKKWFDTFRALWVRAGLRPRCITERRDHDPETCGGIHWGTTAHPAVVTPELLR
                     GFKELGCAHLDYGLESFSDAILTSIDKGSTAKLNAQAVIETMKAGIRPIPNQIIGFPE
                     ESFDSIRGNVVAWKKLGIRAYPFFATPYPGSEWYSLFKEKILAQYDHRLEDFLLDLGD
                     ATKITAVISSRFNAVELLGLRELMVNLDLRRIDAYERQRESCSTRNP"
     gene            12378..14321
                     /locus_tag="PLT63_00695"
     CDS             12378..14321
                     /locus_tag="PLT63_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97267.1"
                     /translation="MKPTRALWIQKLGPGALLTILLRSLGNRLIVHYDEHQASPGPIF
                     VIGLMEKIGLPLVARAASLCLDRRDEEGEGLAYEVRDKIEQCLATLGRELAWPEHPAQ
                     MNMVRSFLFDSLYKRIAFIEMVREKIRGRAEKEALVDEIYLKNHSLNIFIARLYEQTG
                     LIIKNGGLKNEKPGILLRLLLYIPLLIYGMSTGGKVRANITGDKPAIWVEFEHSSGLD
                     FTFWREHVDLHHYDLVYFLFRGDTPADEATIRLLNRQGCKWVDAHFLAAVRMSGMTFR
                     GLARIFATGWTALRRYPLLIGYLFFLYNLNYAIYASLFRKFQVRILIQHHDTSWRQEM
                     WARAVEAAGGILMGLHWSNYPTVMTQNHLFPFHVFFVWGQAIYDFVRQTGHTSRYLLP
                     AGMWLGASGTDENEDPAPFPPEVDFVLAVFDSSASYNIHQTEATLAAFYRWLLSLAVR
                     RPSWGLIVKSKNWRLDDMASFITGAEGQDNIHRLLADRRLKALAPTASPLAAAARAHL
                     SVCYGLNSAGIVCAVHGHKVVHWDCSRWYRHPFYRDGEQRFIFRRRQDLEAAIIRAAD
                     GDKTVGDFSRWKESYNYFGDFDASRRVGRFMNDFLRDREASGAMPPSLDRAAERYLRE
                     NNIGAPFAGADAVKAVVAELRGA"
     gene            14326..15312
                     /locus_tag="PLT63_00700"
     CDS             14326..15312
                     /locus_tag="PLT63_00700"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOD97268.1"
                     /translation="MKFTIGFETEDLKTLHEYWDSIVATHQWTEGRFTRTFEEKWSEY
                     NGLESVSFSSWAGAALAALEYFQLAGKTVLCPSNTFMATPLSVIKAGAAVEFVDCNRH
                     DLCMSYADLQAKIEAHRPAAVWVVHIGGHVAFEIEAIAALCKKKGIVLIEDCAHAHGA
                     SWRGKRPGSWGEAGVYSFYATKTISTGEGGMLVTADKGLAEFARQYRNYGKPDYGVAG
                     FNYRMSEFAAALGCVQTDRLAEIVAWKRAYAERHLDPRFPNRVLFPAGMESGYYKYIV
                     FDPVERSTGKVYDTPCHRIMKKGGDFPNTDWVAGHHWCPPLYYKGNADGDAG"
     gene            15302..16354
                     /locus_tag="PLT63_00705"
     CDS             15302..16354
                     /locus_tag="PLT63_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97269.1"
                     /translation="MRDKKRILFAATQHGSFNMIHPVLRACRERYAVGCLGPREIEPG
                     DYLCRRVIDEEAVLDDGLGKDFDLIVTGTAPTSDVEHRLWRAARGWGKKSVCLLDMVK
                     HCRERFEKNGELVFPDLIIVADEAAAADVARLGVPRERISVAGSPYLAAVAARRLAAG
                     ERELLREELRLGGKRLITFCTEYIAAAGETEKYGYDELHILNILSAYIAARGRGGFSL
                     RIRLHPRDDRALYVDFFAPPGGGSDWAFEEGDAACRFLQAADVVIGMTSTILTEAHLL
                     GLPVISFQPVGPGKAPHAHNRLIADRLVTTAAELYERLDAVFAGRGGDAPGHSLPSSG
                     DPVKTIMEQFERVLSE"
     gene            16370..17341
                     /locus_tag="PLT63_00710"
     CDS             16370..17341
                     /locus_tag="PLT63_00710"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOD97270.1"
                     /translation="MAFKYGMKAAVFPSQLQDRRESFRRLGLPPVIELHLFGARDVFH
                     PEAAAFMSGVRRLGEACRFIVHFPIMDVETGYLFDAIDDDPDKLTKVLAFTEGIGAAE
                     LVMHCCYGFNRDIPKQEARRGFIERVARWNDLAAPKGVRILLENYGFVWLPPGLGAEY
                     AVSPLDHCFPWDMAILAEAVGKLGLGSVGFLLDTAHAVLSANMFNMRQRHGHLAGDVR
                     FGNIYAEDLAQAEYLTAADFVNGFIEYFHVSDACRWRPDDGLDEMDKYLYTEGLPIGR
                     GNVDFAELLGKVRGDETLIMEIDPENGDHGHNVSQYEAVVRLRKIFG"
     gene            17358..18155
                     /locus_tag="PLT63_00715"
     CDS             17358..18155
                     /locus_tag="PLT63_00715"
                     /inference="COORDINATES: protein motif:HMM:NF015087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HOD97271.1"
                     /translation="MCIIIAEAGENHLGDMDLARKMIDVSREAGADYVKFQYYDAEAC
                     ADSDPEKEWFRKVQLDMDQLRYLYGIARQRGIAFLCTPWDAAKAADLFSLGVRDMKIA
                     SFHITDHDLLKLVNDRADKVFLSTGMSSVAEIDAAVAMLKKVDLYLLHCVSEYPLPDE
                     RVNMRTMDYLRERYRCQVGYSDHTLGIVAPLVAVARGAAVIEKHITLSKTYFGTDHIL
                     SADPLELKMLVEYKRMIVSMLGTDEKTMTDAERQNQEFLRNRFSGRQ"
     gene            18176..20197
                     /locus_tag="PLT63_00720"
     CDS             18176..20197
                     /locus_tag="PLT63_00720"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOD97272.1"
                     /translation="MVDKKKKIVVFDLWETLIFGTEDSAISIFYQDITGEEISPEQQK
                     RCLLIAETEPARFLERFFENVTPSQTSSMLLSLKNPRSPLKEKIIQKFRALMDNDARE
                     IRWVPGAMELLAALKKKYTLVVVSNLWAYQKEFLSHKLAVGDCFDRCLFSCDLGMDKN
                     DILRQLGEILSADTRDMVYVGRSYEYDIVPAVNADISAIRITYENNMIFPEKITRMIE
                     EELDGVAAPIARRSFVAQGGRRDVLLVIPPFYKLLGSHNNRLNMGAAFISSRLDSLHI
                     PNRTYHCDSEPHENYVTRYQMVFNSVEFYDHLAGSEAFPAFEEYFRAHGSDTVFVTCG
                     DILNPSFDSGNWDSSQKIAKIVRKVNPRAYIVAVGPEVGLASEDFDLIVHGEVESLLP
                     AILENHQRGRINGGLLSEHGLKSLPPFRFETMATEISPPSLDTILWRRGCAGTCDFCR
                     VAEINKGKIRCRPRAAVMEDIRIRYENYGIRNFYIIDANFTADKAMALEFCREVTEKF
                     PGITWRVESRYDTLDVELLRSMKAAGCTHLKLGLENALSERHQVKTKRVSLAGAAEWI
                     KVIQEVGINCVIYLMLGGKWFNRAQYEEMFANTKALRADGYTVSLYNPYPNTPAGISY
                     EEWRQRRFTGSHLDIRLIDFWKIPIEVVEAFFNLEIEKGREDKMVRRFI"
     gene            20469..21467
                     /locus_tag="PLT63_00725"
     CDS             20469..21467
                     /locus_tag="PLT63_00725"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOD97273.1"
                     /translation="MKIKAGIVGLGRIASLYEEDARAKVFYPCLTHAGSYARHPAVEM
                     VCGADIDAERRRRFGAQWGVAKLYSDYRQMLAENPLDILSVCTHPETHLEILQAARKS
                     VPVIFCEKPFTRGTAEIEAVARWTEQDDAKITINLYREYDKSHLQVRSLLQRGEYGAI
                     QRVNCYIGKGLRNMGTHLLGYLIHTLGAPERITVLDRRYSPAAREYSYDVYLVFPGDI
                     PCLIQSCDFDKFRLFEMDFVCTEGRIQILDEGLKIVVWEAGENRAETGARELRRVRRG
                     IRSTIGYALGGAVAQLVRLAGAPELIPAVSPQRYLVIQRVIEEIERQGGPGKCTHN"
     gene            21452..22753
                     /locus_tag="PLT63_00730"
     CDS             21452..22753
                     /locus_tag="PLT63_00730"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOD97274.1"
                     /translation="MHAQLALLGGKPLRTKGYPVHATTGEEEKKAVLAVLERAILSEF
                     EGTNNQYFLGGEQVKALEGEWAEKFGVRYAVSFNSATSALFAAVGACAVGPGDEVLIT
                     PFTMSATATAILAFNGIPVFADVELDHYNLDPAGLEERITARSRAIFVTHIFGHPAAM
                     GPIMDLAKKHGLRVIEDAAQSPGALYNGKLSGTIGAIGVYSLNCNKVIQCGEGGIAVT
                     DDDDLAQRLRLIRNHGEAVIAAGMAVGSLVNMIGGNYRMTEIEAAIAREQLRKLDDLT
                     QRRRELVSYLNERLRGIPGLGLPGARPDSTHVYYRYALKIDREVIPIDAPLFVKALNA
                     EGLDFYVSYMKPLYLQPLYQRRIGYGDKGCPFSCPFYRGEVSYERGICPQAEELEKMV
                     ISTEIVRPPQTFADMEEIARGVEKVLAEKDQLLKYAATEGM"
     gene            22757..23521
                     /locus_tag="PLT63_00735"
     CDS             22757..23521
                     /locus_tag="PLT63_00735"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HOD97275.1"
                     /translation="MKRVNAIIEARMGSSRLPGKTMMEILGKPSIELLMERLRRARRI
                     TDIVMATTVNAEDDVIAAFCLDYGVKCFRGSSEDVLARVYHAAREHRTDVIVEVTGDC
                     PLLDPWLIDACIDVFLKADYDYLSNFIEQSYPPGIDVQIFAFAALERMHRAAKEAKFR
                     EHVTLYILKNPELFKMHNVYAPPELHYPDWHLELDEYADYELIKAIYEHLYPKNPAFT
                     TMDIIGLLKEHPEWLELNRQVKRTWEQARGEDVGSK"
     gene            23505..24356
                     /locus_tag="PLT63_00740"
     CDS             23505..24356
                     /locus_tag="PLT63_00740"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOD97276.1"
                     /translation="MSDPSEACEQGDNMENVNCNLCGSSETEFWGKKAGIDIVKCRKC
                     GLIYCNPRPDNEELQAFYSADYFDDGHYAGDELRQRMYEIEIEEILANVARRGRFLDV
                     GCAVGKFLLTLPDTFEKWGTEFSAAAARIGREKFGLNIITGQVRNIELPEKYFDIVQM
                     RGVLEHSQNPYEDLLSMRRALKDEGVLRISQLPNIGSISAVLFRTKFNQVKPGEHLYY
                     FSPETIGAMLKKAGFSITSINYPYLETPYAALARDIIELVKCIFQDRETPAFFGNMMI
                     VYAKKDL"
     gene            24369..25385
                     /locus_tag="PLT63_00745"
     CDS             24369..25385
                     /locus_tag="PLT63_00745"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOD97277.1"
                     /translation="MEKKFSRQDSSKQTEQFSYRVNVNDILSEELGEDFRRYRERFEA
                     AAAFAVEPEYPIHIDFETIFGCNLRCIMCTHAHKDRYPQRQRLMDINLFKGVIEEGVQ
                     YGLSSIGLDQEGEPLLVGNLLDYIAYAKQKGILDIMINTNATLLDREKTEALLHSGLT
                     RIHFSLDAITEATYDKIRIGSNFSRVMENILYFCKRKQELGKVLPITRVSFVKMKENE
                     HEMDDFTAFWTERVDAIAIQEYNRAFPDAGELNPAPGEKRIKNPDFRCTQPWFRVVVL
                     TDGRVLPCCLLGYSLQMAMGNAGEESVYQLWNAKKMKALRRMHREGEYFRNKICKQCA
                     LNFL"
     gene            25401..26867
                     /locus_tag="PLT63_00750"
     CDS             25401..26867
                     /locus_tag="PLT63_00750"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOD97278.1"
                     /translation="MKVLMMEVPTRERDGSVPFGLLYAASSAHRRGHEVRIVDLVKED
                     LRDDELLRMIRDFAPGVIGMGGITSSYGRCKELVALIKKDFPAVPIVAGGVIASVADL
                     LLTRAGVDYVVHGEGEISFPNLIQALEAQADVALVAGISFLRDGQLRRTAHQEQIRDL
                     DAIPMPHYGLLKMENYLETLDKWIAHYLRIEDDVNKSVRKVLAGKRYMFPIITARGCT
                     HKCIFCYRHHRGLRQHSVGYVIAMIRHLHETYGVDVFQINDELTTGSKAWIRAFCAAL
                     KKEGLPIALIVLSARVDNVDAEILSLLKEVNCIMINYGYESGANDILKEIRKGVTREK
                     ALETGLLTKKAGIINIPEIIVGFPSETRATVEETIDFLKRLDTWSFSINTPIPFPETP
                     LWDYAVKRGLIKDKEEFVLGYRRGVFINLTGFSDRELLSLVAKVHYDPYLIWLRRRGH
                     YRLYLKTLLVRLFAVNLRQILPESVFAGLRTVYHRRGV"
     gene            26895..28742
                     /locus_tag="PLT63_00755"
     CDS             26895..28742
                     /locus_tag="PLT63_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97279.1"
                     /translation="MPNKKRNLIVVAAPEPALPMAGMRPGQADDTVVSFSVGAKLALE
                     RDHISCLFPDDLVEVPDLNAIGMENMALANEICAVIDDELRARLAFFRAHGIDLFRHA
                     FFPVKIFFDSLITSYLIFAALLNHAAGGRTTVIAAEYALGRIIGGEHPLVPALAEKVF
                     MPVHEGVSVRRVGRNKAWPDRKDDYREWAGLLKSCWRSWPRGAGKAGKQGGVILLDDR
                     YDVKVWADYCRGEREFYKLFFLPRLVALKSLHSRRVRTAGIVGGDGDDRDIAAAFRAA
                     EKELAGHRIFRGHASLCRFAFACLEGYLRFSLGKNITGLGTAVRKLLADESPLLLCTA
                     ACRMDLKDALIMGVAKSLGVPVATYQEGGGAGYLDWPLFNLDAELADYFFVYGEGVAA
                     SPFFDGRPAGIIPVGSPYLAEVEQRLSTAKRDTRAQGIYIVLDNIKTGTWQHYPFNGG
                     AFFQAYRHQERILRAVGDTGSGRFILKTVRGRENLYASHIGAGDRLRMETRPLEEVLG
                     GAAAFILDYPSTVLQECLLTDKPIALLLDGNQVRLEAAALELLRRRIRIAFDVADFPM
                     TLAALQADVQNGNPMTKNRDFLRRYSRPEKDISKINAILWDAVRSKRSL"
     gene            28739..30229
                     /locus_tag="PLT63_00760"
     CDS             28739..30229
                     /locus_tag="PLT63_00760"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOD97280.1"
                     /translation="MNGYKFILGNIFWLSAVRIFTKVAALLTLPIITYYLSPQDFGVI
                     AMVSVVQAFLSGIFSMGLVSYSSRIIYRYERSDRRECRECLGVVLFYILMFSTVGVVV
                     SSLFIDRLVNFFIKDIIFPHRIYLYIPVLMAMLLAIYGFISDNILSLQINKKIFYLEI
                     VQFVLFMPLQIVGLCYWGFDVWDVIVLQAAVQVAITVYGLWLIREWLSFSWHKLSIFK
                     EAMRYSLPMVPLNFLSWVQDRIDKVYLNNMISLNSVGIYTAGVNIATQYSFLSRPVMT
                     AIKPEISKRLDAQDREIQSDIKDAFLIFVQFSLFLYLVISLFSKEVVQLLMNARFHEC
                     YRIVPIFLLSIILSEATGIFQLKFVFRNQTIWFPVTLFFSALLNVYFNYTLIPIFFVY
                     GAALAKTLSEVILFFITLYISQRLHRTDYGLIRNLWPLLAVLALVYSLDAVALSAAYL
                     FLLKLSVVLLYALTLDYLLSRISRRYRETRNIVTAGIRERMARWKT"
     gene            30254..31018
                     /locus_tag="PLT63_00765"
     CDS             30254..31018
                     /locus_tag="PLT63_00765"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97281.1"
                     /translation="MIVALLLGRKGSVGFPGKNLYPVLGRPLMEYPLLAAKYARNVDQ
                     VYVSTDDERIMALGEKHGARIIVRPPELASRAALGEHAYVHGYRVIKESLGKEGKEIE
                     LIVLLFCNAATILAATIDAGIEILRKNEEYDSAVTVSCYNMWSPLRARKIGADGLLHP
                     FVPFETFGDPATLNCDRDSQGDVWFADMGVSIVRPRCLDNLEEGLLPQKWMGRKIFPL
                     QQWGGCDVDFEWQLAGVEYWLAQQGFSAAATPYEMR"
     gene            31024..32004
                     /locus_tag="PLT63_00770"
     CDS             31024..32004
                     /locus_tag="PLT63_00770"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOD97282.1"
                     /translation="MEKRVQAQANANFYSLWGSSDPEDRTARYDEYRHCWMAYPSRFQ
                     VREFPVHLDLEASSRCNLRCTFCDKLPLLSRDQLGDMDFDLYRKIIDEGEERQLWSVK
                     LSYRGEPLLNPRIAEMVAYAKKRGIVDIYFNSNGMLLTENMADKLIAAGLDRISISVE
                     GVDPVRFEKERVGAKYDRIARNIETLMDLKVRRNCSHPKVRVQTVCLPYVDLDEYRDY
                     WRYRCDEAAAIDYKDAVQRDENLIDPGWACPQLWQRMTVEWNGAIIPCNNDDFRLLSP
                     GNVRNRDIYSCWHDPLVQRAREMHRRGESHLLDACRGCPWRATQVKKNGE"
     gene            31994..32746
                     /locus_tag="PLT63_00775"
     CDS             31994..32746
                     /locus_tag="PLT63_00775"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOD97283.1"
                     /translation="MENEHGRQRSCLVVGASGAIGSAVVRRLARKETTMGLHCFKNPG
                     SVASLCRDLGIDANRGHIFQADLGSEKACSGLVAECRQALGSLDLLAICHGDVGWKGW
                     RDLEWADWETIFRQHCLAAYTLATHALPSMIAAGFGRIVYLSSIAPKYAGSSRSIHYA
                     AAKGALEIAMRGLAREVARTGVRINGVRAGFVMTPQQRTGRTEEEQEERIAKIPLGRA
                     GKTNEIAAAFEYLMSDDADFITGEIITVAGGD"
     gene            32743..33639
                     /locus_tag="PLT63_00780"
     CDS             32743..33639
                     /locus_tag="PLT63_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97284.1"
                     /translation="MTVLLQPAMETPSPLQTQIRRLVGKLLVRSLRASGAQRGYGELK
                     QRLEEIIPDISNQYSFLQVNPQDVFLQEKLRNQHAFQMELLLKAMDIYVQDAKREIVH
                     VVDVGDSSGAHLRYLNELVKEKGIAIRSMSVNLDPEAVKKIQAQGMAARQCRAEKLHL
                     LKDGLSADIFISYETLEHLFDPIGFLHDIAIHSSCSYFVITVPYVRRSRVGLHHLRRR
                     SPDPVYAENTHIFELCPEDWGLIFNFAGWEIVCQDCYTQYPSAGLLKLTKYLWRRKDF
                     DGFLGVILTRRPEKAAQYRSWS"
     gene            33656..34582
                     /locus_tag="PLT63_00785"
     CDS             33656..34582
                     /locus_tag="PLT63_00785"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOD97285.1"
                     /translation="MGTILITTSSFAAGTPELLDDLKDRGFPYVLNPYGRVLTGDELV
                     GLLDRHRPVGILAGTERIDGAVLGAAKDHLRVISRVGVGWDNVDLAAAAALGIRVYRT
                     EHVLTQAVAELTLGMILSALRNLAGHDRRLREARWQKQMGSLLAGKLLGIIGFGAIGQ
                     RVGTLARTFGAEVIYYDPCPQAAVTWAGPVSRAELMERAEIISLHAGGREVIMGDGEL
                     KSCRRGVILINTARGTLVDERALVRHLASGQVGFACLDVFEQEPYAGPLLAFDNVLLT
                     PHIGSYAREARAEMESRALANLYRGLKETGCL"
     gene            34579..35472
                     /locus_tag="PLT63_00790"
     CDS             34579..35472
                     /locus_tag="PLT63_00790"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOD97286.1"
                     /translation="MSKKNVVVIGGSGFLGSHLADALTDRGYRVTVFDVRPSPWLRPE
                     QEMIVGNVLDEGEVRKALAGAHYVYHLAGIADIGKSVESPRETLEKNIIGSANVFDAC
                     VQAGIERLMYASTIYVYSQHGSFYRVSKQTVEHMLEAYYEQYALKYTILRYGSLYGPR
                     SQEWNGMKRYITQAVRDGVIVYPGTGEERREYIHVKDAAQLSIAALAAEFINQRLILT
                     GTHALNSRELLRMIQEIMNEHVRIEFKKEGRDPVHYDITPYRFTPREGKKMVSNVFID
                     IGQGILELIEEVYREIGSGRQ"
     gene            35483..36145
                     /locus_tag="PLT63_00795"
     CDS             35483..36145
                     /locus_tag="PLT63_00795"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HOD97287.1"
                     /translation="MKVVWLSHVLAPDTPLYGGGDKIGFTHPRSMARGDSCNTTLLGL
                     SSHAGTHVDVPRHFLPAGGAVEHYPPETWLFHSPLLIEISVPPGELIRPEDVTPKLPL
                     DQPIDILLVKTGFSLRRQEEIYWRQGPGLSPALGEYLKNGISGLRAVGVDFLSVSSLA
                     RREEGREAHRALLEREILIFEDLALGNLQPKERLTKIIALPLRFQGGDGAPCSVLGWV
                     EG"
     gene            36149..36790
                     /locus_tag="PLT63_00800"
     CDS             36149..36790
                     /locus_tag="PLT63_00800"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HOD97288.1"
                     /translation="MPIEAIIFDFDGVLVESLDVKTAAFARMYEKYGPETVAQVVDYH
                     LRHGGMSRYEKFRYYEKILLGHHIGPGEEKRLGEMFSALVVEAVVHAPWVPGAYDFLS
                     AHYRTQDLYVASGTPEPEMIAIVKRREVEHFFVSVHGSPASKAEIITDICLDHRYDRD
                     CVLMVGDSQTDYEGARKAGILFVGRKSGSVSFFPPEVTVLPDLTGLHDYCVAL"
     gene            36841..38517
                     /locus_tag="PLT63_00805"
     CDS             36841..38517
                     /locus_tag="PLT63_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97289.1"
                     /translation="MTPEQAKRLNEVADRTRVHYNDMISRMAIGKEDDLDWWVSGIAG
                     RNTYVCSLFVECCHVVLIRDLLADQDIAEIVTASRPLAKTIRSVCRAQGAAVRVVAKE
                     SFFEPLKILWKSAWDCGRDMARQIYRFWLCRIRFPSRKGPLPANCTLVELFVLDGSFA
                     AGDFQDRYYENFGGCLRQEEEKDHVYLANLAVRFKNLAGTVERLRAATKRFLIQEEIL
                     QTSDFVHAWSYPLRCWPLFPRRALLSGIDVTPLLRRAWLKHLFSSRSLEAILKFGLVK
                     RLKKSGATLRLVIDWFENQDMDKAFNMALRRYFPETAVVGYQGFDTQKYFLCAYPTEV
                     ERRSGVLPHSLAVCGPALMEERRQFCRDLPVETAPAFRYMNVWRGETRKTEAPFFRVL
                     IILPLEAQSAMEILRLLLEAIEAGPGERVRFFLKPHPAMRLESIYGDVAHLTSLLKVV
                     GGNISAYWNQTDLIMGNTSSALLEAAAMGIPVIVIGSRRGITTNPLPESLSGDIWRLA
                     YTKEELLLSLDHFMTSAGKDQKRFAEIGGQIRDRYFSPVTRTAVLKLLRR"
     gene            38573..39511
                     /locus_tag="PLT63_00810"
     CDS             38573..39511
                     /locus_tag="PLT63_00810"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOD97290.1"
                     /translation="MKQGYGHKDKAVRILITGGTGLLGAALQYFAPGDIACFSVYYPE
                     RPLPLPLPFPMRAADVSNPEQMRSIFAWARPDLVIHTAAIGSVDYAQSHGDETAMVNV
                     GGTKVVAELCRIFQAAVVYISSNAVFDGENPLYAETDPVNPINRYGRLKVDAEDVIRN
                     SGVPWAIVRPILMYGWPYPGERDNPVVWWVRSLESGKGIKVVDNVFSKPLSVWSCAEA
                     VWTIVRRSAAGIYHIAGRDHISLYEFALVTAEVFDLNPRLIEPVPDSYFPEIAPRPRD
                     TSFDTTKMETELGLKPQGVKEGLAYMKSRRPPDDEK"
     gene            39501..40553
                     /locus_tag="PLT63_00815"
     CDS             39501..40553
                     /locus_tag="PLT63_00815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOD97291.1"
                     /translation="MKNKKMLMISTDYYGIERSIARAFARNGFEPVFINARMDSLERL
                     LSKFSRVFPKTKRICNPFIRAALQKENRQYISKARIHSPAFAFIVKGDAVFPETLRYL
                     RGELGIPCLSYQWDNPFPTYIGTAGGNDYRDVNFRNSLNDYDHIFIFDDHYTEELREK
                     GGKKVSYLPLAADDELFQHLDMSEDERAKWSFDVCFVGMPYDNRIEILNALSGFHLGV
                     FGDYWERYRDKMVGDYYRGKASGETVRKIYGASKIVLNINHPQSRHGVNARTFEIPCC
                     GAFEIVNHVHGLEDLFAVGKEIVCYKSEEELKSLVTYYLAHDEEREAIVAKGHQRVMR
                     EHTWFNRVKDVIELAG"
     gene            40550..41563
                     /locus_tag="PLT63_00820"
     CDS             40550..41563
                     /locus_tag="PLT63_00820"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOD97292.1"
                     /translation="MKKNIALFMASVLATCLVLEALLRVVYGNQPVFIYPQVMHVPTS
                     YGYKLKPNQQGTYTLDKPVSSNSYGFRDYEWPVPKQRGTYRIMCLGDSLTFGNAVRGE
                     ETYPKVLERKLKDLNANYEVISAAVGGWATFIELDFLKAEGLKYEPDAVIIGFYMNDF
                     TVRCDASQNLTNEGRWDARPSWLQWLPYRYIFLIKRSALITYLRDRIAVVWEGGNDFG
                     SRLLKNQIDLDNDESVKVTYEYLTEIKSICDARGIKCILASIPAINYFWFPRGSVKYM
                     AHLEKFCATNGIEFVDLAEGFWNREETDKLYLYPWDNHLSPAGHELAAAQLLSRLQKT
                     IGF"
     gene            41601..42791
                     /locus_tag="PLT63_00825"
     CDS             41601..42791
                     /locus_tag="PLT63_00825"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOD97293.1"
                     /translation="MTPIRVLIHEPYPFGKIAGNLRTQSYIIRLTDQKRFQLSLLSSF
                     ETEFTRRSAGWGVETLIVPPSRRLNRYGGECLRDGFWEKTLSAFDLIRYNWNLSKILK
                     QKKIDLVYCNSIRSILTVGLAARFAGVPVFLYVKGELQNPLLDPLGFILASRICFFCA
                     ANRDDKYPLLVALFAGKIGILRIGIDLEEIREIERGDKTSLSRELDLDGSYVNCVYVG
                     QLYPPKGVHFLIEALSMVKDEFPRTRLYILGDHIIDAYKDYREELTGLIERRGLREMV
                     FFTGWRPDALAIVSLMDILVHPSLSEGFGRAVLEGMALGKAVIASRVGGLRELIEDDV
                     NGLLVEPGNPSMIAAGLRRLLADRSFREDMGEEARRRVFSDYEITGRVRELEDIWSGM
                     TLRK"
     gene            42860..44797
                     /gene="asnB"
                     /locus_tag="PLT63_00830"
     CDS             42860..44797
                     /gene="asnB"
                     /locus_tag="PLT63_00830"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOD97294.1"
                     /translation="MCGICGEYSFTGATVEQAHVEAMVDSLQHRGPDARGIYCQGEIG
                     LGHTRLAIIDLSPGGNQPLWSADRRFCIVFNGEVYNYREIRGELIKQGCRFSGASDTE
                     VVIHAIQCWGMDRALKRFIGMFAFALWNESERELILCRDRAGIKPLYYSCDSSRLLFG
                     SELKSLCAHPTLVKELDRTALGQHFINGYILAPRSIFKDVYKLLPGHYLVIDRFGRMT
                     MKAYWRLADIVRDSYQGSFSAAAEQLESLLLDAFRYRLVSDVPVGMFLSGGIDSSLVS
                     AILRKKANADILNITIGFQEKAYDEAAKARKVSEALDLKQIVHYVSEQEVQDSLDRFC
                     DIYDEPYGDTSGIPTYILCRLARRHVKVALSADGGDEQFCGYEDYRAYADRYRWLGRW
                     PLALRRIVSQTLAHGLPYRLLLSLPGAISRAESFFPQHAARYEKMLRLLKVRSEGDLL
                     RLMNEKAWSEASLPGFLPGASGDLFADTVLDRDYLHSYRAETVDAMMRTDYQAFLRDD
                     ILVKVDRASMAVSLECRDPFLDHRIAEFAFSLPVAYLYGDGEHKRILKKILRKWVGED
                     IISAPKRGFMIPLYYWLRGVWEPFVREYLSPEKIRKVGILDEKKVQREVSGFYRHHGG
                     RAEKIWMMMNFQMWAEKWYVN"
     gene            44787..45602
                     /locus_tag="PLT63_00835"
     CDS             44787..45602
                     /locus_tag="PLT63_00835"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOD97295.1"
                     /translation="MSTDNIGLELIRKRNVGSGAALRGYILMMMVRPAGVYLAWLALK
                     LGLTPRQVTFASFLFSWVIVFTAAFGGPGGAVAALVMVLFWEAVDVTDGSMARALRKR
                     DNFGGFLDYAAGVVLIAFLPFALGMGVFLWPEGSLDAFLGSMGIAVSVPAVLSLAAGA
                     GIAVISLLMRLVNRVLFIRFGDSFSQWDEKDRNQRMSIKELVNLLVRNLETTGGLQAL
                     IFLLAAVLGWLEIALCGYLVFYLLLLILFMRQTYQTYGRCEDYYVVEVKRRDN"
     gene            45709..47922
                     /gene="sat"
                     /locus_tag="PLT63_00840"
     CDS             45709..47922
                     /gene="sat"
                     /locus_tag="PLT63_00840"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF003166.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HOD97296.1"
                     /translation="MKKDHKDHKDHKDKKEILCTLGPASLNDYTLARLAELGVDLLRI
                     NLSHTKIDDLPGIIGFIQERTSLPICLDTEGAQIRTGDMAGETLHLPENEMLLIPGGG
                     GRSDAPQLSFYPPDIASFFVEGDLVSIDFNSVLAQIVENSAPGLRLKILIGGRVGPNK
                     AVTILNRDVLLPVLTEKDRQALEIGQRYGLRHAALSFANRGADVDVIRRHVGEDTRVI
                     SKIESTAGFRNLKEIAEKSDALLIDRGDLSRQVPIDMIPRMQKLIISRAKKVGVKVYV
                     ATNLLESMVKSSNPTRAEVNDIFNTLCDGADGLVLAAETAIGSYPVHCAMMVRKMIQQ
                     YDEHLNNTFLPDLDGKGSLLLIEPHGGRLVNAWQENPAPEEMAGLIPLDVDVSVLMSA
                     EQIALGTFSPLTGFMTKEEMETVLREYRLPSGVIWPLPIVLQVPGKMAGKLRAGDKVA
                     LRLKGTKEIYATMDIQQIFKPDLEQTARDTFGVTDRNHPGVALLYQRGEWFLGGPVQL
                     VRRLPSPYKHYELTPRQVRTIFAHRGWSKVVGFHTRNVIHCAHEHIQRLALERTHCDG
                     LFAHPVAGPKKKGDYNFDVIIRGYELMIQKYYPPGKVMIGAFQNYSRYAGPREAVFTA
                     LCRKNFGCSHFIVGRDHTGVGDYYQPGDVERLFERVGDLGIVPLFFPTQHYCEACHEY
                     VEQCRHGDDHLLNISGTAGRKMLARGKRPPEWFMRKEISELIIREAREGKEVFVA"
     gene            47919..48461
                     /gene="cysC"
                     /locus_tag="PLT63_00845"
     CDS             47919..48461
                     /gene="cysC"
                     /locus_tag="PLT63_00845"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HOD97297.1"
                     /translation="MKTALVFWFTGLSGSGKTTVATAVKSRLEAAGRTVLILDGDDVR
                     NRLHIQLGFSVADIIENNRLIADLCLEASGGYDIILVPIISPYQSSRAHARSLLGDRF
                     YEIFFAADLDTVMRRDVKGLYKLARENKLNNLIGFSPSNIYEPPDSPDLTIDSRIETI
                     EQSTAALYGFVNEKWQEFSS"
     gene            48643..49635
                     /locus_tag="PLT63_00850"
     CDS             48643..49635
                     /locus_tag="PLT63_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97298.1"
                     /translation="MEINDFSVCFWLEKRGDERGRGYYVKIPKMDLHKKEARNICPLS
                     QADRALGKNEYDSLSSLAKLDLPDSLAVAFVKPVAYLADDNAIVTERVAGRPILASFR
                     RCALRKRIDPRGQADFMDDCLDRLGKTVRFYHRASEKRMATVPYETFHKIDKYLCRLQ
                     SRGRERKFLDQLDRVLKAMGAPTGGAGAALTLKGLDIRNILADGNGRCFILDPGKSKV
                     DFPEADLARFIVTCRIIFWGTPLLFLRLSPGYQYEKRFLDGYYGDEIPHQSRLLGLLI
                     IKELLKHWYLANVALDLKTWPGLIKMILRKTYIDPFYKEQLRREVEGLGAGKRL"
     gene            49616..50290
                     /locus_tag="PLT63_00855"
     CDS             49616..50290
                     /locus_tag="PLT63_00855"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOD97299.1"
                     /translation="MPESDYERQTRRAYRDRYKAAEYRRQHTDQLTWARFATAREKRG
                     VAALLRLCDLGPQEIILDIPCGTGVLAETLAEFPFRVVAADISREMMRLAVYAYKKGQ
                     CRGFVQADITCPPFKNDAYGCVVVLGFMHRVPPLIRAESLRALCRLTERFLILSYSLD
                     SPAQRFKRRMVRFANQCHRFAPYPLKREELRREVHREGLAIRKIMPVVPMLSSEVLLL
                     LEKIPS"
     gene            50305..52221
                     /gene="asnB"
                     /locus_tag="PLT63_00860"
     CDS             50305..52221
                     /gene="asnB"
                     /locus_tag="PLT63_00860"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOD97300.1"
                     /translation="MCGIAGIFSAFSQSKESLAVTARKMAAALKHRGPDDEGCWTDAA
                     GALALGHRRLSIIDLTRDGHQPMVSSCGRFVIVFNGEIYNYLELRDELRKEGFAFHGH
                     SDTEVLLAALSHWGTEAALARVAGMFAFALWDDRERRLTLVRDRAGKKPLYYMIASRT
                     LYFASEIKAFKVLENVDLSVAPEAIYHYLTFGYVPAPGTVYRGIEEIPAGHYVVFNSD
                     LSAASRPYWVLSRSNRRERISFAAAVDEAEVLLKEAVRIRLRADVPVGCFLSGGVDSG
                     LLTAVAALQMDRKLQTFTVAIEDGLFDEGPGAALVARQYGTEHHVLTIKPDIRELLPQ
                     LIQAYDEPFADASAVPSLCIAAEARKYVKVVINGEGGDELFCGYRRHLAMKFYDQFGA
                     LFRLIPEAIFALANRILPPPGDFRTAYAFAHRYLRGLGKTPYDRYIAWCVDGFDEREK
                     GDLYRCRPLIRESSPQVLAAKAADHASDAPFDQFMAWDFFLNMHDDMLVKMDIATMAH
                     GLEGRNPFLDHRLIEWAFSLPRDILLADLRTKPVLRSLAKRYLPAEISLAPKRGFEIP
                     LIKWLREDLRDMVYDACLTRDGIVLDLFDRRYVNDLLDEKLPLDPDRWSKRVWILFML
                     AMWGNMNENRLLLS"
     gene            52196..53356
                     /locus_tag="PLT63_00865"
     CDS             52196..53356
                     /locus_tag="PLT63_00865"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOD97301.1"
                     /translation="MKIAFFCPDDLSTVIFAKSFLKFIAGMDHIEAYTISPVLMNYID
                     QLDKLAIRHYPVRLNRYVDPWSDLDCFRSVYNICRREKFDVLINFTTKPNVLGAIAAR
                     LAGVEKIVCAVRGLGGPFLPQSDLKGHLLQGIVKSLYRLACRMSDIVWFTNQGDMDFF
                     LKTGLVGDEKVVKTTNALDIVEYSQNCIDGDELDRLRQELSLRGDEKLVVMVGRLIWS
                     KGIREFIETAASLVDERPDIKFVLIAPEERGHPDEVPAAFVQEKGKIDNFFWLGFRKD
                     VKNIYALSDLAVLPSYYNEGGYPRALLEPMAFGKPVITTDLPQCRGPVEHGRNGYLIP
                     PRDTRALKEAILRIFHDDRKRERMGEYSRIKIEREFDDRRVVKTILNNVGAI"
     gene            53353..54168
                     /locus_tag="PLT63_00870"
     CDS             53353..54168
                     /locus_tag="PLT63_00870"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOD97302.1"
                     /translation="MKASGSSFHTVTEKPGLKATREQLERIYHRYRFAADYARAASVL
                     EVACGAGIGLGYLACSARRVVGGDIDEVNLEIAKGIYRDWARGEESAATPEIIWLDAH
                     ELTFPDGSFDLLLLFEAIYYLAKPELFVTEAKRVLNRNGRLIIGTVNKDWADFHPSPY
                     THRYFSVPELYELLRRDFSSVVLYGGFSIPEPSRLQMVVSALKRIAVRLNLIPGSLAA
                     RTYLKRLFLGPLRALPAQLQEGMTDYEPPVAISAVRPDRDHKIIYAVATNGRT"
     gene            54165..54797
                     /locus_tag="PLT63_00875"
     CDS             54165..54797
                     /locus_tag="PLT63_00875"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOD97303.1"
                     /translation="MIKRGFDIIVSSLLLVVFSPLMCLVAVMIKGEDSGPVFYRGART
                     GKNGRPFRIYKFRTMVVDAEKRGASSTAADDHRITRTGRFIRKYKIDELPQLINVFRG
                     EMSIVGPRPEVKHYTDMFTGEELGILSIRPGITDWASIWNADEGAVLAGAADPEQAYM
                     ELIRPTKLKLQIKYVRERSFWMDLKIIFLTLRAVVQPESQAVREMRRGLR"
     gene            54826..55803
                     /locus_tag="PLT63_00880"
     CDS             54826..55803
                     /locus_tag="PLT63_00880"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HOD97304.1"
                     /translation="MKYSRELLKDFYRRMLRIRLCEETFVAPILSGEIRCPCHLYSGE
                     EGVAVGVCAALTDGDYVFGNHRSHGHYLAKGGSMEKMAAEIFCREEGCSRGRGGSMHL
                     IAPEVGMLGAAPIVAGTISLALGAALASVIRRDGRVAVTFFGDGATGEGVLYESMNFA
                     ALKKLPILFVCENNYYATHMPVRECRVDRDIFEIAAPFGIESRQVEGNDVLAVYDAAT
                     AAAEKCRRGAGPVFLEFLTYRYRGHVGPDDNIQGEHTDIRPPEEIDIWMKKDPLILLR
                     DYLLAGGLFSSGELDGIRAEVEAEVEEAFVFARAGAHPKTEELERYVFA"
     gene            55790..56770
                     /locus_tag="PLT63_00885"
     CDS             55790..56770
                     /locus_tag="PLT63_00885"
                     /inference="COORDINATES: protein motif:HMM:NF006667.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HOD97305.1"
                     /translation="MSSHRILSYSLAINEALHQTMAADPAVFLIGQGVKSPWYVGNTA
                     QGLRERFGDERVIDTPVSENAMTGAAVGAALAGMRPVVVHPRMDFMFYAFDPIINEAA
                     NWYYMSGGSLPVPVVFWGILNRGGEQAAQHSQALHGMFAQAPGLKVVMPATPYDAKGL
                     MVAAIRESNPVVFIDDRWLYRLEEPVPEEIYTVEIGRARIVREGTDVTVAASSFMVQE
                     ALQASELLAEAGIAAEIIDLRTIKPLDGETLLASVKKTGRLVVADGGWRSFGVAAEIS
                     ALAAERAFGALRAPVARVTLPDCPAPASTALENAYYRNSTDIIQAVRSIL"
     gene            56893..58239
                     /locus_tag="PLT63_00890"
     CDS             56893..58239
                     /locus_tag="PLT63_00890"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOD97306.1"
                     /translation="MDRQVKFVEPAKLYGMIKEELDAAYYEVMSKGDLIDRGQLKRFE
                     ENLAAFVGTKYAVGLNSGYDALHMSLRAAGIGAGDAVIVPAHTFVATASAVVNVGARP
                     VLVDVGKDFNIDCDRVEELLATADRDGEGRIKAIIPVHLSGYMADMARVMELAERYRL
                     VVIEDACQSLGAGMERTNTVHRQQQAGGDRQEETGAHHEARRRRKKLAKQSGDLFGEA
                     TGAVAIGAAAPKATVTGSVSPAPEKISDGWGRAGSWGLTGCWSFYPFKILGGYGDGGA
                     ITTNNPEIALFATRMRYNGEDRQTGEYHGHGFTCLLDNLQAAFLDVKLRYLPQWIIRR
                     QAIAARYRAALSDLSDLLLPHYDDPRRDHVYQNYTVRSRQGNDFSEYLKSRGVEVLTQ
                     FRKPYYRHEALKLEDRDFPETEALSREVCSLPMNVEIADDEVDYVITVVRSFYGKT"
     gene            58261..59418
                     /locus_tag="PLT63_00895"
     CDS             58261..59418
                     /locus_tag="PLT63_00895"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOD97307.1"
                     /translation="MNLRLSERHAWVLQSEIRNMSIECDRVGGINLSQGVCDTEVPPV
                     VRQGAREAMDQGINTYTRYDGLVELRQAIADKQRRFTGMAVDPEGEIVVSAGATGALY
                     CTCLALLNPGDEVIIFEPFYGYHISTLVATGATPVYVPLCPPDWSFQRESLERAWTSR
                     TRAIIVNTPANPSGKVFTAEELQTIAAFAVHHDLFVFTDEIYEHFLYDGCRHLPPALL
                     PGLGERTITISGLSKTFSITGWRVGYALGDARWLQTIGHFNDLVYVCAPAPLQIGVAR
                     GLNALGDDYYEGLARQYLHKRDMICRALTDAGLPPLVPQGAYYVLADISRLPGGNSKE
                     RAMHLLRETGVAAVPGSAFYHEGGGEDLARFCFAKDDPVLAEAAARLRTLK"
     gene            59415..61262
                     /locus_tag="PLT63_00900"
     CDS             59415..61262
                     /locus_tag="PLT63_00900"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HOD97308.1"
                     /translation="MKKLIQNKHFYYMLLIDAALVAASYICAYLLRYEGQLPDFEWRR
                     IIHILPYLVAMKLLCFTLFGLYRGMWRYTSLEDMKNVLKATVVSSLILVLAVLYIYHF
                     RGYSRAAFIIDWLLTLLFVGGVRIGVRLALTNNIGTFWLLAKKGKQDKRLLIIGAGAA
                     GEKAVREILENPGLKLHPLGFLDDDPIKQGKAIHGVAVIGKVEDIGTLPEDFDEILIA
                     IPSARGEQMRRIVRFCEGTGKRFRTMPSIGELIDGKVSVKALREVRIEDILGREEVHL
                     ERELLRVSYEGKRILITGAGGSIGSELVRQVCQFHPAGVGLLDFSEFNLFQIEMTQRQ
                     YFPHIPVETYLTDIRDPAAVRITMESFRPEVVFHAAAYKHVPLQELHPRETIYNNVAG
                     TRNLASAAMAAGAERFVLVSTDKAVRPTNVMGAAKRVAEMLLATLTGHSATRFVSVRF
                     GNVLGSSGSVVPIFQQQISQGKPVTVTHPEVTRYFMSIPEAAQLILQAGAMGQGGEIF
                     ILDMGKPVRIAAMAREVIRLHGLEPERDIPIEYIGLRPGEKLFEELITVGEGIMPTAH
                     EKIKVIQGNQGDYRLLADEIDALIAVADTYDAGAIKEKLRGIVPEYTPQ"
     gene            61259..62371
                     /locus_tag="PLT63_00905"
     CDS             61259..62371
                     /locus_tag="PLT63_00905"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOD97309.1"
                     /translation="MIIGESFSLPGKLRNVLVIQLGDIGDVVWSLPAFQAVCAALPRA
                     EIFLLVRKGIGSLLEADPAITGIFEVERYPGNIVERLMRQIPFLVRIRKVRFGLVFDL
                     RSDERGAFMARWTGAPYRASLWAATAPWYRNRLFTHLVYPTNENIRVRLGASEQTLRI
                     VRQFGIPTPETTPRLALSPTAVDHMKDALAARDILAEGEGKNGGPRGFVTISPFSRWA
                     YKEWPLERWSEIAAWLGREYGLISVLVGAAGERARAEELVKTTSGVLFNLAGETTLGE
                     LAALLSLSRLHVGVDSAAPHIAAAVGTPTITIYGPSDWRDWAPVGDKHQVVTSEMDCS
                     PCHRKGCEGSGRSRCLENIPVERVREAIKKKFLPPR"
     gene            complement(62418..62588)
                     /locus_tag="PLT63_00910"
     CDS             complement(62418..62588)
                     /locus_tag="PLT63_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HOD97310.1"
                     /translation="MAIGKELLEILACPKCKGEISLTEAGDGLICQTCRLLYEIRDDI
                     PIMLIEEAKPLP"
     gene            complement(62749..63810)
                     /gene="waaF"
                     /locus_tag="PLT63_00915"
     CDS             complement(62749..63810)
                     /gene="waaF"
                     /locus_tag="PLT63_00915"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HOD97311.1"
                     /translation="MNILIVKLSAIGDVVHTLPSLAALRRLYPQAHITWVIEAAAADL
                     ITGHPCLDLVIVSHRKRWQREAKSGRVRIIMEEIVSFINELRSRPYDLVIDFHGLFKS
                     AVLVGLSGGIRKLGYDSMQEGSGLFYNEKIPEDMSKHAVDRYLDFPAYLAVKAGKAPV
                     AGPHTFLIPWDEHNEQNVTTLLTKNHLDEEAAFVAINPVALWPTKLWREDLFAALADR
                     ILRELGIPVVFTGSRDEQLYIDAIAQHLPAGRIVNLAGMTSLKDLACLYRRAALVVTT
                     DSGPMHIAAAVGTAVVALFGPTDPRRTGPYGLHHTVIRKELPCSPCFRKTCSTRECME
                     EITVEEVWEGVKEKLGRGK"
     gene            complement(63980..64555)
                     /gene="gmhB"
                     /locus_tag="PLT63_00920"
     CDS             complement(63980..64555)
                     /gene="gmhB"
                     /locus_tag="PLT63_00920"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: protein motif:HMM:NF006506.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HOD97312.1"
                     /translation="MPKKTSAAVFLDRDGTINEEVGYLDRLDKLRLLPGAGEAVQLIN
                     RSGMKVVVITNQSGIGRGLFDEVFVGAVHAEMSRMLGEAGAVIDGFYFCPHHPTEGIG
                     GYRRSCSCRKPAPGLLLRAASELAIDLSRSYMIGDMAKDVEAGQRAGAKGILVRTGYG
                     KDMEAGSSAPDHIASDILDAVRWILGQGKTR"
     gene            complement(64722..65912)
                     /gene="waaF"
                     /locus_tag="PLT63_00925"
     CDS             complement(64722..65912)
                     /gene="waaF"
                     /locus_tag="PLT63_00925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HOD97313.1"
                     /translation="MKDTTVWEPLRVRGNKRLPREGMERILIRGVNWIGDAVMTLPAV
                     AAIRKTWPQANISLLAKPWVADIYRETGMVDELIVFEEPGRHAGPAGKLLLAAELRQR
                     CFAMAILLQNAIEAVIIARLAGIPIRAGYNTDARSWLLTHAVRRSRAIRRVHQISYYL
                     EMVKALGCLDPGDELQLDLQAETASLADSLLPEPAGGNALFIGMAPGATYGPAKRWPV
                     ERFTSVATTLHRQYREAVFILLGSEQDREVTDTIAAAMPDRCMNLAGRTSVREVMALI
                     ARCHVFLTNDSGLMHIAGALNVPTVAIFGSTNPATTSPPGRRNVIIRKPVDCSPCLKT
                     VCPTDFRCMNLIGGEEVTAAINRIITAQHVSGLSVPVGEAAGAGGKDASLIVLPPGEG
                     TSEA"
     gene            complement(66010..66888)
                     /locus_tag="PLT63_00930"
     CDS             complement(66010..66888)
                     /locus_tag="PLT63_00930"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOD97314.1"
                     /translation="MKKEKSTTIIIFLFKAIPLTLRRWLFRGLFRLFYHASAKQRLIT
                     LHNLRRAFPEKDLKEVMDIAKGAYRHLGLVAADFFEIPFINRDNVHRWVTFEGLEHID
                     ATFAKGKGLLSIVAHFGNWEMMTVAFPMMRQPMNIVYRPLDSPILENLTAWVRTRNGN
                     TVIPKGGSGKTITRLLGENKALGILSDQNVSVREGVFVDFFGRPACTAVGLAVLAMRT
                     GAPVIPAFMPRMPDGTYRFIIQEAVSIDDTGDYEKDLFTNTQRFTKVVEAMVRRYPDQ
                     WFWLHQRWKTKQCQME"
     gene            complement(66885..67985)
                     /gene="lpxK"
                     /locus_tag="PLT63_00935"
     CDS             complement(66885..67985)
                     /gene="lpxK"
                     /locus_tag="PLT63_00935"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HOD97315.1"
                     /translation="MNSIHSICHELWYRRDPSGWRRSLLLAMIPPSWAYGAATALRNR
                     RYDSGAVRAERLPRPVVSIGNITVGGTGKTPMAILVARGLLARGFRPAVLSRGYGGRR
                     SGSGNVVSDGRQIFLSPAEGGDEPALMATSLPGIPVITGPERVVSGRLAINKFAADVL
                     VLDDGFQHRRLARDVDILMIDGQNPFGNRRLLPAGPLREDPRTALNRADIVVKTVVEE
                     LPRRVSADDSPADSTRPVFRAAYEATGLCPGDGGEALPPEILQDKKILAFTAIAAPEK
                     FQATLEAMGARIIKFLAFPDHYFYKEGDVRKIAGAATKQAVEMIVTTEKDGVKLAAFQ
                     EFYRTIHILSVGLRIDPGEEAFFGALIKLMPR"
     gene            complement(67957..69702)
                     /gene="msbA"
                     /locus_tag="PLT63_00940"
     CDS             complement(67957..69702)
                     /gene="msbA"
                     /locus_tag="PLT63_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HOD97316.1"
                     /translation="MDVFKRLLKLAKPHSGRFLMAMLCMLVVGTSTSALAFLVKPALD
                     GIFLKKDAQMLIWIPLVVIFIYLAKGLCSYGQMVLMNYIGLRVVADLRGSLYRQIQKQ
                     SLSFFTRHPTGILMSRITNDAGTLQGAVSDAVTTMFKDTFTLIGLIFVIFYRDWKLAV
                     IAILIFPLIIYPIAKFGKKMRSVATRTQITMGALTTLLHETISGTRIVKAFGMEEYEN
                     KRFSEENERFFKLNIKAVSINAAASPFMEFLGGIGIAAIILYGGFQVIKGGATPGTFF
                     SFLTALIMLYEPVKRLTNMNNTIQQGIAGAQRVFSIIDMEPEIGDGPDAVALPKITRS
                     IDIENVSFAYEETPVLKDIDLSIRAGEVVAFVGMSGGGKTTLVNLIPRFYDVTTGRIA
                     IDGQDIRDVTVESLRGQIAMVTQQTILFNDTVRNNIAYGDIQRSEEEIVGAARAAHAH
                     DFIMQLPQGYDTIIGEAGTKLSGGERQRISIARAILKDAPILILDEATSSLDTEAEIE
                     VQKALDNLMAGRTTLIIAHRLSTIRNADRIIVLVNGRIVEEGNHEFLLAKRGEYYKLY
                     CMQFTEDELHPLDMP"
     gene            complement(69703..70848)
                     /gene="lpxB"
                     /locus_tag="PLT63_00945"
     CDS             complement(69703..70848)
                     /gene="lpxB"
                     /locus_tag="PLT63_00945"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HOD97317.1"
                     /translation="MAIYEKKVLIVAGEASGDLHGANLVRAMLESDPGLVFYGIGGPK
                     MEAAGVRCLARAGDMAVVGLTEVFSKLGMILGIMRKLKRSLWEEKPALIILIDYPDFN
                     MPLARAAKKRGVPVFYYISPQVWAWRRGRIKQLRQCVDRMAVILPFEAEMYRREGMAV
                     EFVGHPLLDGINTKYHRSEAIRRFDLREEGSIIGLLPGSRPGEVTKLLPEMLAAAGII
                     KQELPTAQFVLPLADTLDRDGMEEMIRPCQEKLNVKIIGNEIYEVLAVCDAAMVASGT
                     ATLETALIGVPMIIVYKISLFTYLMGRIFVNVDHIGLANIIAGKTVVPELIQQDVNPP
                     RMAKEILAILNDVERKNRIKRELAGIREKLGAPGASRRAAHLAMEML"
     gene            complement(70946..71914)
                     /locus_tag="PLT63_00950"
     CDS             complement(70946..71914)
                     /locus_tag="PLT63_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOD97318.1"
                     /translation="MNELRIGVVGIGHLGNYHLQKYALIPDCRITAVADVVAERAHRA
                     AAQYGCLAFQDHRDLLGRVDAVSIAVPTVSHYETAVTFLEAGVSALVEKPITVTLAEA
                     DALIAAAGRNNAVLQVGFIERFNPAVIALAKVIQQPLFIEAHRLHPFFERGTDVDVIL
                     DLMIHDLDVILHFVKSPVAAIDAVGVSVLSEMVDIANVRLKFASGCVANITASRVTGK
                     TLQKIRFFGIDGYHAVDYNKRDLVSLSKKISDSGRLDIFSNAVEVCQHDPLEAEIRSF
                     VAAVQNGARPVVSGEDGRNALDLALNIAREIKKAKDEILLSIQHGK"
     gene            complement(71914..72687)
                     /gene="lpxA"
                     /locus_tag="PLT63_00955"
     CDS             complement(71914..72687)
                     /gene="lpxA"
                     /locus_tag="PLT63_00955"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HOD97319.1"
                     /translation="MNIHPTAVVSGAANLEEGVVIGPYSIVAADVNIGKNTVIGPHVV
                     IDEHTDIGAGCRIFQFASIGAVPQDLKFRDEVTRTIIGNHNTIREFVTIHRATAADIG
                     VTIMGDGNLIMAYCHIAHNCKLGNRIVMANAANLAGHIHVEDNAIIGGMTGIHQFTRI
                     GAHCIIGGCSAVGKDVPPFTMASGNMAKLYGLNLIGLKRRNFAPETIQALKLAYRIIF
                     RSSLLLKTALEKVRTDVPDIPEVRQFIDFIQNTQRGICR"
     gene            complement(72684..73739)
                     /gene="lpxD"
                     /locus_tag="PLT63_00960"
     CDS             complement(72684..73739)
                     /gene="lpxD"
                     /locus_tag="PLT63_00960"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOD97320.1"
                     /translation="MEITLNELAAHLDGRVIGDGTVLIKGVNGLDEAREGDLSFLANP
                     RYQEKIITTGAGAVLVKPGVECPGKNLLVVTDPYAAFGRALELFHRRQRPAPGIHPQA
                     IIATGAEVDPEATIYPQVYVGAGAIIAARAVLHPGVVIGDNAAVGAESILYPNVTIYE
                     GCIIGQRVILHAGVVVGSDGFGFAVPGQENRKIPQVGIVQIDDDVEIGANTTIDRATL
                     GRTWIQKGVKIDNLVQIAHNVVVGEHAIIVAQVGVSGSTRIGRGVLIGGQAGIVGHIS
                     IGDHAMVAARAGISKNVPAGQIYGGAPQQPYREWLRAASSIPKLPEMRNKIAALNRRV
                     AALEQIISKKEGETTDL"
     gene            complement(73747..74277)
                     /locus_tag="PLT63_00965"
     CDS             complement(73747..74277)
                     /locus_tag="PLT63_00965"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOD97321.1"
                     /translation="MKKTIWAIGAAIAFSIFLVAPTASQAEKIGFIHVEQVLMNSQVG
                     KATMEELKKNYDKNRANIQNKEKELQRLKDELEKQRPVLTEKALRDKEQSYQKKFRDY
                     QDMVKDANDEMQIKRQEFLGKNVPEILKIVNAIGEKENYTLIIDLSTLPVAYHAKGNV
                     LTQRVIDEFNKIKKSK"
     gene            complement(74305..76902)
                     /gene="bamA"
                     /locus_tag="PLT63_00970"
     CDS             complement(74305..76902)
                     /gene="bamA"
                     /locus_tag="PLT63_00970"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HOD97322.1"
                     /translation="MWWIGPAAAAPPEPVAVAIIPFTVHSKSPAAFLQDSFYDNLARE
                     LGKAKNVRIIEKGKILPLLAGQKTDDKAITAVGKAVGAGYVVSGTVTELGETLNVDMR
                     IFDVAAGKYLPPVSAQGKGPESILAIAGQLRGDILIKTSATQRIAAIGFQGNKKIENS
                     AISQVLKSTPGGIYSETNLNEDIRAIYKLGYFDDVAADVADTPEGKTITFIVKEKGLI
                     TDIQIKGNKKLDTKDIEAVLTFKTRQALSKEKIATSMEKIKALYDDKGYFNAEISYAI
                     EREREKDIRVIFTIKENSRLYIKKISFEGNRSFRDNELKKIMKTSEQGFFYFFTDSGL
                     LKKTELKQDINRLNAFYLNNGFITAQVGDGIISYDKKGVYIKIPITEGQRFRVGRVDV
                     TGDELKVKRSELMEKLNIRKQEHFDRGAVIKDMELLTQACNDEGYAYAEVTPITRVND
                     NALNVDINYDLKKGNLVYFNRISLIGNTKTRDKVIRRSLQMTEGELYSSTKMKDSYKN
                     LMRMRYFEEVDFQSEKGPDDSLADVNIRLKEKPTGMVSMGAGYSALDKAMVMAQISQQ
                     NLFGRGQILSLKANIGSVSSYYDISFTDPWLFDIPLWSKFDLWNSSRIYDTYDLGTKG
                     GGAVFGYPLWEKVTGYVSYTLAFTDVTNISPFASNYIKQQAGNATTSSMGFTISRDTT
                     DDDFFPTTGSKNAATILQAGWLLGGTVSFTKYTGTSTWFFPLPLETVFDIRGRIGFLQ
                     PNEGKELPVYERYYLGGINSLRGLRDIGPRDPVTGDLIGGKTMLSFNADFVFPLIKNA
                     GMKGVVFYDTGNAWENGYHLDDMRQTAGVGIRWYSPVGPLRLEWGYVLDRKEGEDPSR
                     WEFMIGMMM"
     gene            complement(76943..77620)
                     /locus_tag="PLT63_00975"
     CDS             complement(76943..77620)
                     /locus_tag="PLT63_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD97323.1"
                     /translation="MIQVKDLKKTFIKDGNRINVLKGLDLTVRKGESLALLGVSGAGK
                     STLIHILGALDRPTSGEVRFDNVNIFTWDEDKLADFRNRKVGFVFQLHNLLPEFSALE
                     NTMMPALIHGVSHREAKQRAESLLVSVGLAHRFTHKSGELSGGEQQRVAVARALIMAP
                     DVLLADEPTGNLDTETGKKIEDLLVELNVTKKITLVVVTHNKNLADRLQRKVGLRDGR
                     TYDISKT"
     gene            complement(77620..78897)
                     /locus_tag="PLT63_00980"
     CDS             complement(77620..78897)
                     /locus_tag="PLT63_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HOD97324.1"
                     /translation="MPFELFISLRYLKAKRKQVFVSIITFLSIGGIALGVAALIIVLA
                     VMNGFETDLRNKILGMNSHVLLMEYTGPMKGHREVAKKVEAIGDVMATTPFIYSQAML
                     KSQNGVTGVVLRGLAPESAFRVISLGKIKEGKVEHLDPRTRTSLTIPPDRIELPGILI
                     GKELAKMLGAYLYDTITVVSPLGISTPMGMVPKMKRFIVAGIFDSGFYEYDSTLAYID
                     LKDAQEFLNMPGIVTGLEIKVKDLYQADVVAKNIEKVLGYPYWARNWMEMNKNLFAAL
                     RLEKRVMFIILSLIVLVAAFNIITTLIMVVMEKSKDIAILKSMGATARNIMKIFIFQG
                     LTIGAVGTIIGTVAGLSIAFNLAAISASIERLLGIKILPGDVYYLSELPSKVNYGDVA
                     MIVIGAMTICFLATIYPSRRAAKMDPAEALRYD"
     gene            complement(78901..80385)
                     /gene="lysS"
                     /locus_tag="PLT63_00985"
     CDS             complement(78901..80385)
                     /gene="lysS"
                     /locus_tag="PLT63_00985"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOD97325.1"
                     /translation="MEESELIKTRKEKIAALRAAGIDLYPNEVRVGHTSAELRTRFDA
                     LDNDALAEVKDTFSLAGRLMAIRNFGKASFISIQDRKGRLQGFIQKKTLPEEEYDLFR
                     RLDVGDIIYIEGKIFKTKTGELTIEAGRLRLLAKAVHSLPEKWHGLTDMEMRYRRRHL
                     DLIMNPDVREVFHRRSRIIKLIRDFMDGHDFLEVETPMMQPRAGGAAAKPFKTFHNTL
                     GMDLYLRIAPELYLKRLVTGGLERVYEINRNFRNEGISTFHNPEFTMMEFYLAYATYE
                     DLMRFTEELFLFLAENVFGAPRFTYQGTEINLSPPWRRISVREAVETHLGIAPEIMAD
                     RRRLLATAAEMGLEPEENAVVGKILMMIFEEKVEKELIQPTFVTQYPVEVSPLSRRNP
                     RDPGFTDRFELYICGREIANAFSELNDPEDQRERFLMQLREREAGDEEAHEMDEDYVR
                     TLEYAMPPTAGEGIGIDRLVMLFTDSASIRDVILFPQLRIRDEQ"
     gene            80575..80994
                     /locus_tag="PLT63_00990"
     CDS             80575..80994
                     /locus_tag="PLT63_00990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOD97326.1"
                     /translation="MINKVILVGRLGGDPEVRYTPDGLMITNFNLATDEQRKDKNGER
                     IQKTEWHRIVTFGKLAEICNSYLTKGRLVFIEGRIQTRAWEDKEGVKRYTTEIIAGNM
                     QMLEAKGQQKGPAANAAEAPAPSYFSPESLPGDDVPF"
     gene            complement(81319..81504)
                     /locus_tag="PLT63_00995"
     CDS             complement(81319..81504)
                     /locus_tag="PLT63_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOD97327.1"
                     /translation="MFGIGMPELLVILVIILIIFGAGKLPEIGGAIGKGIKNFKKATR
                     EPEEIDATPENKKIEPK"
     gene            complement(81650..82504)
                     /locus_tag="PLT63_01000"
     CDS             complement(81650..82504)
                     /locus_tag="PLT63_01000"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HOD97328.1"
                     /translation="MKENQDKDKNTTQMEPATIDTESMYPDLRGIRKAKGLSLKDVYE
                     ETRITSTNLEAIEAGRFNALPEPVYTRMFIKNYTRYLEVDGAPVLKRYEAYLAFQHKP
                     TGRPTEKRRDEQPVKKGKKKYLLGSFLALGLLVVIVAGIFFHGAESPDHVPFPPGVQK
                     APPNREAQHPSPLPPSMPKERMAVTTLSPSPPAGPSTPSTPPPGSADKDLMLSIAATE
                     KTWLRITSDGGKAEELMLRKGEAIERTARKAFQITVGNAGGVQVRFQGKQLPDLGKRG
                     EVKHLKLT"
     gene            82791..83618
                     /locus_tag="PLT63_01005"
     CDS             82791..83618
                     /locus_tag="PLT63_01005"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOD97329.1"
                     /translation="MMRMKRCPEQTDGVEGAYRYFAMAVAAEAGRYLRDNLNNRHQVR
                     FKGVIDIVTEADQQAEDMIVGAIHRQYPHHDILAEESAAFTTGSPYRWIIDPLDGTTN
                     YAHGYPVFCVSIALEWEGNCILGVVYNPMLEEMFVAEAGRGAYLNDRSLVVSKTGILS
                     EGLLATGFPYDIRWSQENNMNFFQAMAVEVQAIRRAGSAALDLAYVAAGRFDGFWELK
                     LHPWDTAAGCLLVREAGGTVTDLRGEAFLLTSPHVVASNGLIHDQILSVLQGVDKTS"
     gene            complement(83714..85201)
                     /locus_tag="PLT63_01010"
     CDS             complement(83714..85201)
                     /locus_tag="PLT63_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97330.1"
                     /translation="MEKGCLIKGSLVLLIIIVFICPSLAGAQPPMDLQQDDLLKEMEK
                     VSEEGDQGATPAATPTKPATILSKLVDNFHGSLRLRGMHFWQNPPDREGADNRNDFGN
                     TLLKISDKTGYKNLDLNIGAWVEYGNEENTYSGTVFSEFWQDLDRRRRIFYINDLYLN
                     YSRDNFNFLVGKKTITNGMSTLYLPSDRMRPQDLNDPLDVKDLNIWQAKLDWEVAESS
                     TMTLAFLPVYQPQKQPSETSRWMGERKQGDNTEFDLYDAGNVNMKDDVAKVTDTNFGY
                     FARFKKKGFHGWDFFASFYHGPNPFWVVREERIPGPFPGITLIQRVKEVVKVDDYAAG
                     FSTTYESWEFHGELVYNYSYDRKDDDYFNYVAGFTYTFDDFAKKIWLEKIDVTVEYAN
                     EIITRFQNAENYVASSKKSRLGRNDILSRINFEYSNKLSFQFICNFQLTRGEEGRFQR
                     LEAKYKLRDGLITKLAGEFFGGQSDSMYGRWVRNDRIILEMEYSF"
     gene            complement(85363..86175)
                     /locus_tag="PLT63_01015"
     CDS             complement(85363..86175)
                     /locus_tag="PLT63_01015"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HOD97331.1"
                     /translation="MAKRAIIWVFGILFLFSAAGVSAQMTGRQVMEKQKERHKVKTEI
                     GKEDMMLVEKSGAKENRQVVRYAKEVGRDLHRYLVVFHSPGDIRGTALRTWEKKGGDN
                     DQWLYLPAQKKEQRIAKAGKKNYFMGTDFTYEDMEPEDLDNFNYNIIRSEQFNHEGKA
                     HDCYVIEALPANAKKKNESGYAKRIMWIEKANLTTLKTDFYDKRNKHQKTQFNYKFET
                     ISGTVMRPKQTLMNNIEKNHKTVTATRNRQVNINLSDGTFTEQFIISGKHLQ"
     gene            complement(86224..86601)
                     /locus_tag="PLT63_01020"
     CDS             complement(86224..86601)
                     /locus_tag="PLT63_01020"
                     /inference="COORDINATES: protein motif:HMM:NF017415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HOD97332.1"
                     /translation="MLDMAKKAALIGTGMALATTDKIREVVEDLTHKGESAQTDAQTE
                     ARKAVEVLTEKTRQTRESIEGETEKIMTDFSAFWYYTNLADELEERVEKIVAVILKKL
                     HIPERQELEDIKKRIENMEREEV"
     gene            86803..87909
                     /locus_tag="PLT63_01025"
     CDS             86803..87909
                     /locus_tag="PLT63_01025"
                     /inference="COORDINATES: protein motif:HMM:NF020130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HOD97333.1"
                     /translation="MKTAATQGKTPPITGKQSSPPFSSRFESIGVKVPEQRLSTRDLM
                     SGVKMWGKFDLERLTGIRERRVCSETEDSYVLSLDAARDCLKHSAYQAADLEMIICCS
                     ISKYKDGNAFVFEPPLSLYVKEALGANNALNFDIANACAGMLTGVSILNDFIRRGVIK
                     RGMVISGENITSISRNAVPRVKTIFSDQLASLTVGDCGAAVILERSQVGQAELKMSNF
                     ITLARYSDLCTGWACPRAPGAQMVTNARKIHQVAIADMPPILEKALKEAGLEFGDIDH
                     VVPHQTSIRAIRSGDKHLAHYFGVRAKNIVVNLQEYGNTASTSHFLALYRYLNERLFR
                     PGEKLMFIALASGLIIGVLIFTMDHLVERYGIEN"
     gene            87893..88780
                     /locus_tag="PLT63_01030"
     CDS             87893..88780
                     /locus_tag="PLT63_01030"
                     /inference="COORDINATES: protein motif:HMM:NF020130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HOD97334.1"
                     /translation="MGSKIEAVALNTPGFFRRGSIDMEIRAAKECLKKTRLDPNMLGL
                     LVNTGVYRDRNIGEPAIATLIQKEIGANEIFAGGGSTFSFDLTNGGCGMLTGMMVVDG
                     YLHSGDIDYGMVVTADADPVPGRTEGFHFTPAAAAILLSRGTEGHGFLHYRTATEGRF
                     IDDLTGRIEWMEYKGKMRNILVVKQAEGFAASCARFARENLDSFLKELSLTMKDIDLV
                     IPSQSPRGFLEELKKKRDLELKLVDVTDKLADVHTAGIGFALERAMSTGRFAAAKNCL
                     FLTVGAGLTASLALYRNQA"
     gene            88867..89814
                     /locus_tag="PLT63_01035"
     CDS             88867..89814
                     /locus_tag="PLT63_01035"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOD97335.1"
                     /translation="MRFTPADIDADNISLPFDIAPHRRLYPFADYWLKAPGFIYHYLD
                     EGQGDPVVAVHGNPTWSFYYREIVKALRPDYRVIVPDHVGCGLSSRPSEKDFTYTLTS
                     HVDNMESFLDYLDLKNNVTLIVHDWGGMIGMACAARRPERIARIVILNTWAFVIPPDK
                     GIPWQLSFLRHFPVLPALLVRGLNAFSYLATFLGVEKDMPADVRTGLTAPYNTWKNRI
                     ATLRFVQDIPLYPGDRSYPLTQWTDEHLHLLGGIPMMICWGQKDFVFDEVALAEWRRR
                     FPEAEVHVFPRGGHYILEDEGETVVALIRDFLERHPLMT"
     gene            89884..91557
                     /locus_tag="PLT63_01040"
     CDS             89884..91557
                     /locus_tag="PLT63_01040"
                     /inference="COORDINATES: protein motif:HMM:NF006754.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031956 - medium-chain fatty acid-CoA
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid CoA ligase family protein"
                     /protein_id="HOD97336.1"
                     /translation="MGRVEEVEDKTVATANLSEHLTRVAHLMPDKKAVICPAAPGAHD
                     FIHWTFRELEEEADRLAHGFHQAGIVKGVKTILMVRPSLELFAVTFALFRVGAVPVMI
                     DPGMGRQKLVENLSSISAEAFIGIPLAHILRVLSPGKFSTVKVKITVGRRLFWGGYTL
                     DDFRRGPWRPFTPVPAHPGERAAIFFTTGSTGPPKGVVYEHGMFDAQVRYLSTHFGYG
                     RDEVDLATFPLFSLFDAVLGMTSVIPDMDPTRPAQADPRKIIHALETHNCKSMYGSPA
                     LLENLARYGAETGKKLPAVKRIITAGAPVRPDLMEAVHCLLPADARIHTPYGATEVLP
                     VSDIDSREVLTETRKISESGGGTCVGRPLAGMEVRIIGITEEPLSRIREAVILPPGHI
                     GEIAVRGPVTTKEYFQNPKATLLAKMRDDDGGVWHRMGDVGYLDDKGRLWFCGRKAHR
                     VETAGGTLYTIPCEALFNRHPRVRRSALVGVGVSGNKRPVIIIELKAGDSGRDKDRLT
                     EELLAIARDNAITRMINTVLYHHGFPVDIRHNAKIFREKLTVWAEERLK"
     gene            91594..92643
                     /locus_tag="PLT63_01045"
     CDS             91594..92643
                     /locus_tag="PLT63_01045"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOD97337.1"
                     /translation="MPVNAGEIKGDSPARAARGSVALVTGGGGFLGGAIVSHLLAAGY
                     AVRSFARSDYPELRRRGVEVIRGDLADPEAVRRACADCAVVFHNAARAGVWGDFDSFY
                     RPNVVGTKNILAACGRAGVGRLVFTSSASVVFGDADLAGVDESVPYPDRPRSPYTATK
                     AEAERMVLEADRPALRTLSLRPHLIWGPGDTQIIPRILAQARAGKILRVGDGKNIIDT
                     TYIDNAAAAHLLAAQALAENPWAAGRPYFISNGEPVNLWDFVNRILALAEIPPIRRGI
                     PKTVAVLMGGVIERLHLVLKLPGEPRMTRFLAEELATTHWFDIGAARRELCYEPRVSM
                     AEGLRRLQTWLQTRN"
     gene            complement(92669..94543)
                     /locus_tag="PLT63_01050"
     CDS             complement(92669..94543)
                     /locus_tag="PLT63_01050"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOD97338.1"
                     /translation="MSLEKWKTLYPDKFVPEAEIFSHIHRGDTIFIGSACAEPQHLVQ
                     GLIRYVKSYPKAFFDAEVIHIRSLGVAPYATEKFKQNFRHNSFFIGDSTRDAVNKGVA
                     DYTPVLLSETPELFRRGLARVDIALIQTSLPDEHGYLSLGISVDIVKAAAEEAKIVVA
                     QVNHFMPRVHGDSFIHIRDIDYIVPYDELLLEYTKGVNSEIVMGIGKYVARLVEDGDT
                     IQVGYGSIPNAVLANLKDKRHLGVHTELIGDGIIELMKTGVIDNSKKTLNPGKTVTTF
                     CLGNQDTYRYLHDNPAIEFRTIDYTNNPLIIARHDRMTAINSALEIDLTGQATAESIG
                     RIFHSGVGGQADFMRGAVLSRGGKSILTLQSTAACDVVSRIVPMLREGSGVTLTRGDI
                     HYVVTEYGIAYLHGKSIRERAMELIGIAHPKFRPWLIDEAKKLGLIYRDQAFIPGVRG
                     EYPEHLETYRTTKKGINILFRPIKISDEPLLKDFIYSLSDQSLYRRFMSARKDMPHEV
                     LQKLVIIDYTVEMAILAIMFREKAEEIVGVGRYYIDETAHAAEVAFAVKDTHHRMGIG
                     TELLAYLTYLAKRQGLLGFTAEVLSENQPMLHTFEQGGFDLERKNVAGLWELKMTFRE
                     "
     gene            94863..96953
                     /locus_tag="PLT63_01055"
     CDS             94863..96953
                     /locus_tag="PLT63_01055"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOD97339.1"
                     /translation="MKSFFYPKSMVVVGASATKMNLGQIILLNNKQCGYQGRLYGVGS
                     QAGEVAGIPVYTQIDDLPEIPEVAIMLTPARTIPALLEDCGRKGIRRVVIESSGFSEY
                     AHGDHSLEQEVLDAAARHGIRFIGPNCVGTVNFDIKMMMPFAFFKAPPSGGRLAMIAQ
                     SGGVGGTYLHALPENAIIPGKFVAVGNKLQLDEVDFLEYFLMDEETDVVTLYLEGFSR
                     GRMFHDLAMGSHKPIIVQKSNRSEMSAKIAQSHTTALSAGDDVVNGMFRQAAVIRAED
                     EGEFLNAIKIIRLPLMKGRRVAVLSRSGGHAVLSADACDKYGFDMAPFPSAFLKKLKT
                     LYHTRVIAHQNPLDLGEIFDYTIFTGILEETLKLDNIDGVLFNHLYASAYEAQMSRTF
                     LAGVAKLVDQYGKPVAVAMLSEPAELLDVARSQPYPIFTSPTQAAAALDMSATYHERK
                     TRRDNRGAPPPISLDEARIAAVRERCRRERRIPWTDEALEICRAAGLTPAPAWTVRRP
                     EEGETLAVRFPVAVKLLSREASHKTDVGGVRLSVRTRRSLRKVLGEMAERFASIADGF
                     LIQEMAGPGVECFVGGRRDPVFGPVVMVGLGGIFIEVFKDTAIRLAPVTKAEAADMVA
                     ELKAYPLLRGFRGREAADIAALEEVICRVSTLLAATPEIAELDLNPVIVHPAGKGVSL
                     VDARIFFAQRQLED"
BASE COUNT        19556 a        27197 c        29266 g        21228 t
ORIGIN      
        1 aagttatata gtgacgaaat ttcgtcatcc ccgtaaatgt gcccggtgag ctttgatgac
       61 atcgtaaaaa gcgcaaaaaa tgttccattt gggacggcat cgtaaaaaga tccgcaggcg
      121 aggcgcgcga atcctgagga atgaggcgta ctgggacgta cgtcgccgtg acgagggatg
      181 aagcgcaacg aagcatccgg actttttacg atgccgtgag ctttgtgtag ctgcggaaag
      241 atggttcgca agcggcaaat caaatgtttt tcgccgtttc gtgattgaac ttaacgcctc
      301 gtaatgaatt aagatttata cgttacctat ggacctgaca aaactcagca tcttggtaac
      361 cggagccgac gggttcatcg gcagccacct ggtggaggcg ctgttgtccc gaggctgccg
      421 ggtccgtgcc tttgtgttct acaattcctt caacagttgg ggctggttgg aggccctgcc
      481 caaggagctt cgggacggcc tggatgtctt tgccggcgat attcgtgatc cccacggggt
      541 ggccaaggcc atggcgggta tcgacgtggt cttccatctc gccgccctga tcggcattcc
      601 cttcagctat cattctccgg acagttatgt ggatacgaac atcaagggga cgttgaatgt
      661 cctccaggcg gcccgggagt ccggttgtga ccgggttttg atcacctcca cgtcggaggt
      721 ctatggaacg gcccaatatg tacccatcga cgaacggcat ccctgccagg gacagtcgcc
      781 ctatgccgcg acgaagatcg gcgccgaccg catggcggag agcttttatc gcagtttcga
      841 caccccggtg acgatcgtcc ggccgttcaa tacctacggg ccgcggcaat cggcccgggc
      901 ggtcattccc accatcattt ctcagttgct ggggggggtg aggaagatca agctcgggaa
      961 tttgtccccc acccgggatt tcaattatgt gctggatacc gtgcggggtt tcattgccgt
     1021 agcggagact ccggccgcca tcggggaaga gataaacatc gcctcgcagc aggagatctc
     1081 catcgggcgg atagcacggg agataataga ccaaatcgat cctgccgcgg tcattgaatg
     1141 cgaagacatg cggattagac cggaaaagag cgaggtggaa agattactgg gcagtaatga
     1201 aaagattcgg agcctgacgg gctgggagcc cgcatatgac ctgaaagagg gactccggga
     1261 aacgatcgcc tggttccagg acgggggaaa cctgcgccgc tataaatggg atatctataa
     1321 tgtttgagaa aacggtggcg ttcataaagt ccctttatcc gggcgaggtt cccgtgcctc
     1381 tccacgcccc ccgttttggg ggcaatgaaa agaagtatct attggattgc atagatacga
     1441 cttatgtctc ctatgtcggg gaatatgtgg gtcgttttga agccaagatc cgggaatata
     1501 ccggggcggc gcatgccgtc gccgtttcca gcggcacggc cgccctccac accgccctga
     1561 tgctggccgg cgtttccccc ggcgacgagg tgataaccca gccgctgacc tttgtggcca
     1621 ccgccaatgc cattgcctac tgcggcgccc ggccggtgtt cgtcgatgtg gagcagacga
     1681 cgctgggtct cgatcccgac aaattggcgg atttcctggc tgcccacgcc cgcccggatc
     1741 cccacggcgg ctgccgcgac cggacgacgg gggggaggat agccgcctgc gtccccgtgc
     1801 ataccttcgg gcatccggcg cggatcgacc gcatcctgga gatctgcgga cactatgggc
     1861 ttcccgtcgt cgaggacgcc gccgaggcct tgggcagttt ttaccggggg cgtcatgccg
     1921 gaacgttcgg ggaggtgggc atcctgagct tcaacggcaa caagcccgtg acgaccggcg
     1981 gcggcggcat gatcatcacc ggcagcgaca ccctggcggc cagggcgaaa cacctcacca
     2041 ccacggcgaa gcggcagcat acctgggaat tcttccacga tgaggtgggt tacaactatc
     2101 gtctgccgaa catcaacgcc gccgtgggct gcgcccagat ggaacgcttt gccggcgtcc
     2161 gggagaacaa gcgcgtcacc gcggagttgt accggcggtt tttcgcggcg tccggcatgg
     2221 aattcgttgt ggaacccgcc cacgcccaat ccaattactg gctcaatgcc ttggtcctga
     2281 aggaccggga ggagcgggat gcctttctcg ccttttcccg gggaaagggt atccagaccc
     2341 ggcctgtctg gtcgctggtc tccgatctgc ccatgtatcg ccattgccgc cgcgatggcc
     2401 tggaaaccgc ccgccgtctg gaagggcggc tggcaaacat ccccagcagc gtgcggatat
     2461 gaagaaacaa atcctcctca tcggcggcgg cggccactgc cggtcctgca tcgatgtgat
     2521 cgaacgggaa gaccgctttg tcgtggccgg gattgtggat gtattggaaa aaaggaatca
     2581 aaccgttctc ggttacccgg tgatcggttc cgatggggat ctgcccgcat tgatcaaact
     2641 gtatccccag gtgctgatta cgctgggtca tatccgttct ccgcggcgga gaatggaact
     2701 gtttggggag ctggaggaca tgggggcgga tttcccggtc atccggtccc ccctggccta
     2761 tgtctctccc catgcccggg tgggggcggg gacgatcgtc atgcatcacg ccctggtcaa
     2821 tgccggggcg cgggtgggaa aaaattgcat cgtcaacacc cgggccctga tcgaacatga
     2881 cgcggttatc gacgatcatt gccatatcgc caccggggcg gtggtcaacg ggggcgtaac
     2941 gatgggcagc ggcacatttt acggaagttg tgccgtatcg aaagaataca cgaccatacc
     3001 gcccggaagt ttcatcaagg ccggcggcat ctatccggaa aaacgatgaa ccgtaccttc
     3061 attatcgccg aagcgggcgt caaccacaac ggcgtcctcg ctacggcccg ggaaatcatc
     3121 gatgcggcgg tggctgcggg ggcggacgcc gtgaagtttc agactttcca ggcggaaaag
     3181 ctcgtgaccg ccgccgcggc gaaggcggcg taccagctcg agaataccgg cggcgatgaa
     3241 tcgcaattcg agatgttgag gaagctggag ttgtcccggg atgatcatcg ggccctctgg
     3301 aggtatgccc aagaaaaaga tatcatcttc atgtccaccc cctttgacga ggaaagcgcc
     3361 gacctgctcg cggagctggg gatggagata ttcaagatcg gatccgggga actgaccaac
     3421 cggcccctcc tcgagcatat cgcccggaag gggaggccga tggttgtctc cacggggatg
     3481 tcttatctct atgaggtcgc ccgggccgtc cagtggctgg aagaggcacg ggcggcggca
     3541 aaggaccccc ccggtccgtc ccgccggacg ttcccctatc ccctggtcct gctgcactgc
     3601 gtgaccaact atcccgccgc gccagcggcg gtcaacctcc gcgccatgca gaccatgaag
     3661 gaggccttcc acctgccggt cggctactcc gaccacacgc cggggatgga gatggccgtg
     3721 gcggccgtcg ccttgggcgc cgtggttatc gagaagcacc tcaccttgag ccgggagatg
     3781 gcgggtccgg atcaccgggc ctccctggag cccgccgagt tcgccgccct ggtccgggcg
     3841 gtcagaaacg tcgaggcggg gctgggggac gggatcaaaa aaccggcgcc gtcggaactc
     3901 ccctaccggg acatcgtccg gaggagcctg gtgttgacca gggatatccg ggcgggcgaa
     3961 aagatcgccc ttccggatct gtccctcaag cggcctggaa agggcattgc cggtgaattc
     4021 cgtgcgattg tcctcggcat gcgggccgtc cgggatctgc agaaggatgc gcacctgtcc
     4081 tgggaggatc tgaaaaatgc gtagaatagc ggtcgtgacc ggatcacggg cggaatacgg
     4141 cctgttgtac tggctcatga aggagatcca ggaagatgtc gacctggagc tgcaattgat
     4201 cgtcaccggg atgcatctgg cgccggaatt cgggatgacc gtaaaggtga tcgaaaggga
     4261 tggctttccc atcgccgaaa gagtggagat gctgctgtcc gccgattccg acgcggccgt
     4321 ggccatttcc atgggtttgg gcctgatcgg cttggccaag gcataccaaa ggctccagcc
     4381 ggagctgatc gtcctcctcg gcgaccgctt cgagatcttt gccgcggcgg cggccgccct
     4441 gcccctggcg attccggtgg cgcatatcca cggcggggag gcgacggaag gggccatcga
     4501 tgagtatttc cgccatgcca tcacgaaact gagttttctc cactttgccg cgacgccccg
     4561 gtaccggcgg cggataatcc agatgggcga ggcgccggac cgggtcttct gttatggggc
     4621 gccgggactg gacaacatcg ggagactgcc cctccttacg gccgccgcct tacgggagga
     4681 gctgggattg accacggcca ggcggaattt aggcgtggtg acctatcacc ccgagacccg
     4741 gggcgtggaa ccggcagcgg agcaaataaa cgccgtcttg acggctgtaa gtggttttga
     4801 tgacatatat tggctattta cccttcccaa tgccgaccaa gggggccggg aactgggcgg
     4861 gatgatcagg gagtacctgg agcggcggcc ggaggtcggc accctctttg cctctttggg
     4921 gcagttgtac tatctctccc tattgaaaaa ttccctggta atggtgggca attcatcgag
     4981 cggccttatt gaagcgccct ccttccggct gccggtggtg aatatcggcg cccggcagga
     5041 ggggagagag cgggccgcca acgtcattga cgtaccccgc tgcaccgtcg ccgcgatagc
     5101 ggcggggatc ggcagtgcca gatcggcggc tttcgcagcg agtatcgaga cgatggaaaa
     5161 cccctacggt catggcgatg cgagcgggaa gatcaaggag gccctcaaga cggttcccct
     5221 ggacaaacaa ttgttgaaga agaggtttta cgaaggggcg gatggaacag aataaattgc
     5281 aatcactcct cgtgcctccc gagctgacga tcaaggaggc gatgcagaaa ctcaacgaat
     5341 cggcacagaa gatcctcttt gtcatcgacg gacggcagcg gctggtgggc acggtgaccg
     5401 atggcgatat ccggcgggcg atcatcggcg gcaacggttt ttccgatccg gtggcgaaga
     5461 tcatgtaccg caaattcgcc ttccttaccc ggggtgcgcc cgccctgctc gccgcggcga
     5521 aagaactgat gatcgcccgg gagatcgagc aattgccggt gcttgacgag gacggggcgg
     5581 tccgggacgt gatcctgtgg accgacatcc tggagaagaa aggcgccgac gtcccggttt
     5641 cccgccatga gaaccgcgtg gtcattatgg cggggggcaa gggcacccgc atggacccgt
     5701 ttacgaggat tttccccaaa ccgttgattc ccatcggcaa gaagaccgtg gtcgagatga
     5761 tcatggacca gttccaccgc tggggctttc agcatttcat cctgaccctc aactacaaaa
     5821 aggagtatat aaaggtcttt ctccaggagg cggaactgcc ctacggggta gactggatcg
     5881 atgagccgga gtttctcggt acggcaggga gcttgtccct cctcaaggat cacctggggg
     5941 atacctttgt cgtcgccaat tgcgattccc tcctggacct ggatgtcgcc gaggtgctgg
     6001 cctggcatcg tgaccagggg gcgatcatga cggtgatcgg ctgctaccac gaggtgaaga
     6061 tacacttcgg cgttctggag ttgggggagg ggcggctgcg gaggatcctg gagaagccga
     6121 gccacgatac gataatcaat accggtatct acgtcctgga gccccgggcg atcgattata
     6181 tccccgctgg tgaagaggtg catatggacg gcctgatcgg gaagatgatc gccgccgggg
     6241 aaaaggtgtc ggtatatccc atccattccg gctggctgga catcggccag tgggatcagt
     6301 acaaaaaggc gatccgcgac tatgacctgt gaacaacccc ggcgggcggc catcatcggt
     6361 ttgggcaaca tcggtttccg cttcgacctc cagagccggc ggcggggaac ctggagccac
     6421 gcggcggcct acacccgcag cccggagacg gagctggtgg gcgctgtgga actggatggc
     6481 aacaaggccg atctctttcg gaaacattac ccggccgtgc cggtatatga tgatatcggc
     6541 cgtttgatga ccgcatgccg gcccgagatc gtttccatct gcacccccac ggagagccat
     6601 tatcccctgc tgaaggaatt gctggtctac ccgctgcggg gggttttctg tgagaaaccc
     6661 ctggcggcaa acgcggcgga ggggctggaa atggtccgcg ccgccgcggc gcacgggatc
     6721 gtcctggccg tcaatcacac ccgccggtgg gaccccggat tcctccgcgt ccaggagctg
     6781 gtgcgtcggg gagaactggg ggcgatccgg gctgtgcatg ccgtttaccc cgggcaggtg
     6841 ttcaatatcg gcacccatct gatggatgtg gtctgcatga tcaccggtcg ggcgccggtg
     6901 tgcgtgtccg gggtggcgtt gcccggcggg ggcgacgacc cccatctcag cggctggttg
     6961 cgcctcgatg acggcacggt ttgcacgata aacgccctgg gccggcggga gctcctcgtc
     7021 tttgaactgg attttatcgg tgcgcgcggc cgggtcagga tctcggacaa cggggagtcc
     7081 ctggagggcg ccgcctttgt ccacagcgac caatatgacg gctacgaggt gctgcggcgg
     7141 cgggaattgc ccgcgccccc ggagcgggat agactggtgg aggcggtgac ggacatggcg
     7201 atggtcatcg gcggccggaa ggacaaggtg aactgcaccg gcgccgacgg tctggtggcc
     7261 ctccgtctcg cggcagccct ggcagcctcg gcgcggcgcc acggccggcc ggtcgccgtc
     7321 gaaggatagg aaaggaaaaa gatttctgag ccgcggcgaa gcctgttttc aaaggtatag
     7381 aaaggggaat tatgagtgaa ttggcattat tagggggaac gccggtgcgt cgcaccccct
     7441 ttcgcagttc cgtggtcatc gacgaccggg aatgggaaga gctgcggaac gtcctcaaaa
     7501 gggaggagtt ttcccgtttc atgggcagcc ccacgccgga tatcgacaag ctcctcgtga
     7561 tgccctcggc ggcggcagcc gacctggaag ggcagtattt cagttttctc ggggggagga
     7621 cggtccggcg cttcgaggcg gaattcgccg ctcgattcgg cgtgacctac gcaatttccg
     7681 tcaacgccgc cacctcgggg ctggccgccg ccctgggagc ggcggggatc ggtccgggtg
     7741 acgaggtgat cacgacgtgc atgtccttca acgccacggc gatgagcatc ctgctgttca
     7801 atgccgtgcc ggtgtttgtc gatgtggatg cacggacgtt ttgtctggac cccgaggcgg
     7861 tggagcgggc gatcaccccc cggacgaagg cgatcctggt ggtccatctc ctgggcaacg
     7921 ccgcggatat ggatgccctg ctggccatag ccgggaggca tggcctgaag gtcgtggaag
     7981 acgccgccca ggcgccggga acgacctacc ggcgccgctg ggtgggcacc ctgggcgata
     8041 tgggcgtctt cagctttcag gagacgaaaa acatgatgac cggtgagggc gggatgatcg
     8101 tgaccaatga tccggcgctg gccaaaaagg cccggttgat ccggaatcac ggggaaggta
     8161 tccccgacga gacctgggat gacgaatccc tgaccaatct tgtgggcctc aacttccgga
     8221 tgacggaact gacggcggcc ctggggcggg cgcaactcgc caagcttgcg gagaacaacc
     8281 gaatccggac ggaaaacgcc ctcttgctcc gggatcgcct cccggacctg cccggactga
     8341 ccaggccgga tatcccgccg gggacggtgc cccacgtctt tcccatgctt tatgacgaag
     8401 cggccacggg cgttccccgg cagaaggtcc tggcggcgtt gcgggcggaa ggcatcccgg
     8461 tgggctcggg ttatctccgg ctcatgtatg aaaatcccct tttcctgagg aagatcgcct
     8521 acggcaagca cggctgcccc tggtcctgcc atctctacgg ccgggagcgc cgctaccttc
     8581 ccgggcagtg ccccgtgggc gaagcgttgc tgcgccggcg cttcctctgg ttttatcata
     8641 tccaccgccc caatacggcc gcggatatgg aggaggtggc cgtcgccttc cgcaaggttt
     8701 ttatgaatct ggaggactta cgggcggcaa caggggactt tacgatcccg tacaaatggt
     8761 gaagctatgg aaaacgattt aaatattgtc ggtatcatcc acgcccgggg ggggtcgaag
     8821 cgcatcccct tgaaaaacat aaaattactg ggggaggaac ctttgatcgc ctacctgatc
     8881 aaggccgccc tggccagtcg gcatctgcgc cgggtcatcg tttccaccga tcacgacgag
     8941 atccggagca tttcccggcg ctacggcgcg gaggtcccct ttacccggcc ggcccacctg
     9001 gcggaggact gcccgtcgga atgggtcagc cggcatgccg tggaatttat cgagtcccag
     9061 gaaggccggc cggtcgatat cgccgtcacc atgcagccca ccacgccgtt ttgtaccggc
     9121 gcggatatcg acgcctgtat cgaggttctt ttggcggaca aggaactgaa atcgtccttc
     9181 accgcaaaaa cggttaccga acgaccggaa tggatgttcc gccggcggga aaaccagcgg
     9241 gcggaactgc ttctggcggg ggccatgaag ggggagcggg ggatctttca atccttgtcg
     9301 gaactcgtca ttcccaacgg cgccgtctat gccacccggc gggataccct ttttgacgag
     9361 ggggtcatta ttgccgacaa gaccggggtg agtgtcatgc cggcggaacg gtccgtggac
     9421 atcgacgaac cgatagactt tttatttgcc gagtttttgc tcaccaaagg agtgatccat
     9481 gagtaagctg ctgaagggaa attatgaaat agaccgcaag gaagacaacg aaaccgccgc
     9541 ggcggcgaac aacgatttta ccaaggtgtt cgaaagcaag gccctggaaa aactgggcgg
     9601 cagcatcgcc tttgtggtca aggcggatca attcgtctgg gatggcgacg atgacgttaa
     9661 gtgaagaaca gttccgtctc ctttccgagg ccaccctgag gaagacggcg gcccggggtc
     9721 tggagcatgc ccgggcgaac cttccctatc tgcaaaagac catccgggac ttgcaggcgg
     9781 aattggcgcc ggcgccccgc cggcccgccc tggtcatctc ggcgggcccc agcctccaca
     9841 aacggaattc gatcgggaag atcaaggacc gcggttttga aggacccatc gtatgcgccg
     9901 acggggcctt ggggcattgt ctccgtaacg ggctgatccc cgagtatgtc ctcaccgtcg
     9961 atcccgaccc gcatcggatc attcgctggt tcggcgatcc ccgcttgacg gaacgccccg
    10021 ccgacgatta ttttcgccgt caggacctcg atcccgccct ccataaggac gaagaacgga
    10081 aaaacgagga aatgatcgcc ctggtgaacc gttacgcgtc ccggataaag ctgatcatcg
    10141 ccaccagctc ctctcccgag atcgcgcaac gatgccgtgc cgccgggatg gaactttatt
    10201 ggtggaatcc tctctatgac gattatgacc gaccggacag cgtttcccgg acggttttcc
    10261 gcctgaacaa ggtttcctgc atggtgacgg ggggaaacgt cggcacttcc gcctgggtct
    10321 tcgcgcaatc catcctccgg agcgccgcgg tggccgtcgt ggggatggac tgttcctatc
    10381 cgccggggac gaaaccggaa aacacccagt actatgagca tttaaaggaa attttccccg
    10441 atgaccccgc ccgggggctg gtcaatatct acaatcccca cctccgggaa acctggctta
    10501 cggatccggg ctattactgg tacgcccaga tctttctcaa gctggtccag atggcgccgg
    10561 ccccgacgtt caactgtacg gaagggggga tactgttcgg cccggggata gaattccggc
    10621 ctctggtcga gatcctgtcg ctttttcacg acggtcggga ctactccgca gcaaccagga
    10681 ccacataatc atggcgaaga ttctctttat caatcccgtg attcgggaaa acgaaaaacc
    10741 cgcccatgtc cctttcggga tggcgcaact ggttgccatt tgcgtccggg cggggcatca
    10801 ggtgcaggta ttcgatgcca atgcctggcg tccgacggac gacgaaatcc tcgcggtact
    10861 gaaggcggat gaatggcggg tggttgccat cggcggcctc gtcaccacct acggcttcgt
    10921 caagaaggtg ttgcggtacg tgaaaaaggc ctgtccccag gccctgacgg tcgccggcgg
    10981 cggtttcctc accccgatcc cgcatgaggt gatggccttg cttcccgaaa tcgatctggg
    11041 ggtgatcggg gagggatatg tcaccttgcc ggacgtgttg gcccgggtcg atgccgccga
    11101 ggaagactgg catgagattc ccggcctcat ttatagagac cgggacggcg tttgccagtt
    11161 gacggccggc cggcccctcc tggaggaggt ggacacgctg ccctttcccg cctgggacct
    11221 gtttcccctg gacatctact tccgcaacag cggcttgttg ctgagcgagg aggccatgct
    11281 ggcgaagcgc catctcggcg tgatcgccag ctacggctgt ccctaccggt gcaagtactg
    11341 tttccatctc ggcttgagcg gggagatcga gattgtggaa ggaccggcgg gacggcggct
    11401 gtgtctcacc tccaagcgga aggtgcgggc ccattcgccg gagtacgtcg tggcgatggt
    11461 cgcggaggcc aagaagagat tcggcatcga tttcgtttcc tttctggatg aaaatttcgc
    11521 cgtcctggcc aagaggaaaa aatggttcga caccttccgc gccctctggg tccgggcggg
    11581 gctgcggccc cgctgcatca cggagcgccg ggatcatgat ccggagacct gcgggggcat
    11641 tcactggggt acaacggccc atccggcggt ggtaaccccg gagttgctgc gcggattcaa
    11701 ggaactgggc tgcgcccatc tcgactacgg cctggaatct ttttccgacg ccatcctgac
    11761 ctccatcgac aaggggagta cggccaagct caatgcccag gccgtgatcg agacgatgaa
    11821 ggcggggatc aggcctatcc cgaaccagat catcggtttt cccgaggagt cctttgacag
    11881 tatccggggc aatgtcgtcg cctggaagaa actggggatt cgggcctatc cctttttcgc
    11941 caccccttat cccggttcgg aatggtattc cctcttcaag gagaagatcc tggcccagta
    12001 tgaccaccgg ctcgaagatt ttctcctcga tttgggcgac gcgacgaaga tcacggcggt
    12061 catttccagc cggttcaacg cggtggaact cctgggcctg cgggagctca tggtgaatct
    12121 ggatctgcgg cggatagacg cctacgagcg gcagcgggag agctgctcga cccggaatcc
    12181 atgaactcct tgataatact ggattaccgc ttttgcagga atgacaatat ggaggcggga
    12241 acagagtttt gcaaaggtct cgcgtcatgt ccgggtcttt ccctgccttt tgcgcgggga
    12301 aaccggcatc tccaccatcg ttcggtccct gggggcggcg tcaggcaagg accccggtta
    12361 cgcaaagcct tccggcaatg aaacccacgc gcgcgctctg gatccagaaa ttaggccccg
    12421 gcgccctctt gaccatcctc ctccggtccc tcggaaaccg cctgatcgtc cattacgatg
    12481 agcaccaggc ttccccgggg ccgatatttg ttatcgggct gatggaaaag atcggtttgc
    12541 ccctggtcgc acgggcggcg tccctctgcc tggatcgccg cgatgaggag ggggaggggc
    12601 tggcctatga ggtccgggac aagatcgaac aatgcctggc gaccctgggc cgggaactgg
    12661 cctggccgga gcatcccgcc cagatgaaca tggtgaggag ttttcttttc gacagcctct
    12721 acaagcggat cgccttcatc gagatggtgc gggaaaagat ccgcggccgg gcggaaaaag
    12781 aagccctggt cgatgaaatc tacctgaaaa atcactcctt gaatattttc atcgcccgcc
    12841 tctacgagca gaccggtctg atcatcaaaa acggcgggtt aaagaacgag aagccgggaa
    12901 tattgctgag gttgctgctt tacatccccc tgctgatata tggaatgtcg accggcggga
    12961 aggtccgtgc caacatcacc ggggacaagc cggctatctg ggtggaattt gaacacagca
    13021 gcggccttga tttcaccttt tggcgggagc acgtcgatct gcatcattac gatctcgttt
    13081 atttcctctt tcgcggcgac accccggccg atgaggcgac gatccgcctg ctgaaccggc
    13141 aaggatgcaa atgggtggac gcccacttcc ttgcggcggt gcggatgagc gggatgacct
    13201 tccgggggct ggcgaggatt ttcgccacgg ggtggacggc cctccgacgt tatccgctcc
    13261 tcatcggcta tctgtttttt ctctataatc tgaactatgc aatttatgcg tcgctgtttc
    13321 gcaagtttca agtccgcatc ctcatccagc atcacgacac atcgtggcgg caggagatgt
    13381 gggcacgggc ggtggaagcg gccggaggca tcctcatggg cctgcactgg tcgaattatc
    13441 ctaccgtcat gacccaaaac cacctctttc cctttcacgt cttctttgtc tgggggcagg
    13501 cgatatatga ttttgtccgc cagacgggtc acacgagccg ttatctcctt cccgcgggga
    13561 tgtggctggg cgcctccggg acggacgaaa acgaagaccc ggcgcctttc ccgccggagg
    13621 tggatttcgt gttggccgtt ttcgattcgt cggcgtctta caacatccat cagaccgaag
    13681 cgacgctggc ggcgttttac cgttggctcc tctccctcgc cgtccggcgg ccctcctggg
    13741 ggctcatcgt caaaagcaaa aactggcgct tggatgacat ggcgtccttc atcaccggcg
    13801 cggaggggca ggataatatc caccggctgc ttgccgatcg gcgcctgaag gcgctcgccc
    13861 ccaccgcctc tcctttggcc gcggcggccc gggcccatct ttccgtgtgt tacggtctga
    13921 attccgccgg tattgtctgc gccgtccatg ggcacaaagt ggtgcattgg gattgctccc
    13981 gatggtatcg gcatccgttt tatcgcgacg gtgaacagcg gtttatcttc cggcgccggc
    14041 aagatctgga agcggcaatc atccgggcgg cggatgggga taaaacggtg ggggactttt
    14101 ctcgatggaa agaaagttat aattatttcg gtgatttcga cgcctcacgc cgggtgggga
    14161 gattcatgaa tgattttctg cgtgatcggg aagcttccgg cgccatgccc ccgtccctgg
    14221 atcgcgcggc ggaaaggtat ctccgggaaa acaacatcgg cgcccctttt gccggcgccg
    14281 atgccgtcaa ggccgtagtc gctgaattac gaggagcatg agatcatgaa atttaccatc
    14341 ggtttcgaaa cggaggacct gaagaccctc catgagtact gggacagcat cgtcgccacc
    14401 catcagtgga cggaggggag gttcacccgg accttcgagg aaaaatggtc ggaatacaac
    14461 ggtctggagt ccgtttcctt ttccagttgg gcgggggcgg ccctggcggc cctggaatat
    14521 ttccaattgg cggggaaaac ggtcctctgc ccgtccaaca ccttcatggc gacgcccctg
    14581 agtgtgatca aggccggggc cgcggtggag ttcgtggact gcaaccgcca tgacctgtgc
    14641 atgtcatacg cggatttgca ggcgaagatc gaggcccatc ggcccgccgc ggtgtgggtg
    14701 gtccatatcg gcggccacgt ggccttcgag atcgaggcga tcgccgccct gtgcaaaaag
    14761 aaggggatcg tcctgatcga ggattgcgcc cacgcccacg gcgcctcctg gcgggggaag
    14821 cgccccggtt cctggggtga ggccggcgtt tactccttct atgccaccaa gacgatttcc
    14881 accggcgagg gaggcatgct ggtaacggcc gacaaaggcc ttgccgaatt tgcccggcaa
    14941 taccggaatt acgggaaacc cgattacggc gtagcgggtt tcaactaccg gatgagcgag
    15001 tttgccgccg ccttgggctg cgtccagacg gaccggctgg ccgagatcgt cgcctggaaa
    15061 cgggcgtacg ccgagcggca tctcgatccc cgcttcccga accgggtcct ctttcccgcc
    15121 gggatggagt cgggttatta caagtacatc gtcttcgatc ccgtggagag atcgacggga
    15181 aaggtttacg ataccccctg ccaccggatc atgaagaagg gcggcgattt tcccaacacc
    15241 gattgggtgg cgggccatca ctggtgcccc ccgctgtact acaaaggcaa tgcggacgga
    15301 gatgcgggat aagaagcgca tcctgttcgc ggcgacccag cacggttcct tcaacatgat
    15361 ccatccggtc ctccgcgcct gccgggaacg ttacgccgtg ggctgcctgg ggccccggga
    15421 gatcgaaccg ggagattatc tctgccggcg ggtgatcgac gaggaggcgg tgctcgacga
    15481 tggcttgggg aaggatttcg acctgatcgt gacgggcact gcgcctacgt cggacgtgga
    15541 gcaccgcctg tggcgcgcgg cccgggggtg gggaaagaag agcgtctgcc ttctcgacat
    15601 ggtgaaacac tgccgggagc gatttgaaaa gaacggcgag ctggtcttcc ccgacctgat
    15661 catcgtggcg gacgaggcgg cggcggcgga cgtggcccgt ctcggcgtcc cccgggagcg
    15721 gatcagcgtt gccggttcgc cctatctcgc ggcggtagcc gcacgccgac ttgccgccgg
    15781 ggagagagag ctgctccggg aggagttgcg gttaggcggc aaacggctga tcaccttctg
    15841 tacggaatac atcgccgccg ccggggagac ggagaagtac ggctatgacg aactccacat
    15901 cctgaatatc ctctccgcct acatcgcagc gcggggccgg ggtggctttt ccctccggat
    15961 caggctccac ccccgcgatg accgggcgct ttacgtagat tttttcgccc ctccgggagg
    16021 cggcagcgac tgggcctttg aagaagggga cgccgcatgc cgtttcctcc aggcggcgga
    16081 cgtcgtcatt ggcatgacct ccacgatcct cacggaggcc cacctgctcg gcctccccgt
    16141 gatcagtttt caacccgtcg gcccggggaa ggcgcctcat gcccacaacc gcctgatcgc
    16201 cgaccgcctc gtaacgacgg ccgccgaact atatgaacgc ctcgacgccg tttttgcagg
    16261 ccggggcggg gatgcccccg gtcattccct tccgtcgtca ggcgacccgg ttaaaacaat
    16321 tatggaacag tttgaaagag ttttgtcaga ataaccccgc gaggccggct tggcttttaa
    16381 atacggtatg aaggcggcgg tctttccctc ccagttgcag gaccgccggg agagctttcg
    16441 gcgtctggga ctcccgccgg tcatcgaact ccatctgttc ggcgccaggg atgtcttcca
    16501 tccggaagcg gcggctttta tgtccggggt gcggcggctt ggcgaggcct gccggtttat
    16561 cgtccatttc cccatcatgg atgtcgagac aggttacctc tttgacgcca tcgacgatga
    16621 tccggataaa cttacgaagg ttttggcctt tacggagggg atcggcgccg ccgaactggt
    16681 catgcactgc tgttacggat ttaaccgcga tatccccaag caagaggccc ggcggggatt
    16741 catcgagcgg gtggcccggt ggaacgatct cgccgcgcca aagggagtga ggatcctcct
    16801 ggaaaactac ggattcgtgt ggctgccgcc gggattgggg gcggaatatg cggtaagccc
    16861 cctggatcac tgttttccct gggatatggc catactggcg gaagcggtcg ggaagctggg
    16921 attgggatcg gtgggctttc tcctcgatac ggcccatgcc gtcctgtcgg ccaacatgtt
    16981 caacatgcgg caacgccacg ggcatttggc cggcgatgta cgcttcggca acatctacgc
    17041 cgaagacctt gcccaggcgg agtatctgac ggcggcggac ttcgttaatg gtttcatcga
    17101 atattttcac gtttccgacg cctgccgctg gcggccggat gatggccttg acgaaatgga
    17161 caaatatctc tacacggagg gtctccccat cggtcggggc aatgtggatt ttgcggaact
    17221 gctgggaaag gtgcggggcg atgagaccct gatcatggaa atcgatccgg aaaacggcga
    17281 tcatgggcat aatgtctccc aatacgaggc cgtcgtccgg ttgcgaaaaa tcttcggctg
    17341 agggggtaga taggaaaatg tgcatcatca ttgcggaagc gggggaaaat cacctggggg
    17401 acatggatct cgcccggaag atgatcgatg tctcccggga ggcgggagcc gactatgtga
    17461 agttccagta ctatgacgcc gaggcctgtg ccgacagcga tccggaaaag gaatggttcc
    17521 ggaaggtgca gctcgacatg gaccagttgc gctatctcta cgggatagcc cgccagcggg
    17581 gcatcgcctt tctctgcacc ccctgggacg ccgccaaggc ggcggacctc ttttctttgg
    17641 gggtgcggga catgaagatc gcctcctttc atatcaccga tcatgatctc ctcaaactgg
    17701 tcaacgaccg ggcggacaag gtcttcctgt ccaccgggat gtcctccgtg gcggagatcg
    17761 acgcggcggt ggcaatgcta aaaaaagtgg acctctatct cctccattgt gtatcggaat
    17821 atcccctccc ggatgaacgg gtcaacatgc ggaccatgga ctacctccgg gagcgttacc
    17881 ggtgccaggt tggttactcc gatcatacct tgggcatcgt ggcgcccctg gtggcggtgg
    17941 cccggggggc ggcggtcatc gagaagcaca ttaccctttc gaagacctat ttcggcacgg
    18001 atcatatcct ttccgccgat cccctggagc tgaagatgct ggtggaatac aagcggatga
    18061 tcgtttccat gctgggaacg gacgaaaaga ccatgaccga cgcggagcgg caaaaccagg
    18121 agttcctgag gaacagattc agtggccggc aataagccgg tgaagaggtt gataaatggt
    18181 tgataaaaaa aagaaaattg tagtttttga cttgtgggaa accctgatct tcggtacgga
    18241 ggattcggct atctccatat tttaccagga tattacgggc gaggagatct ccccggaaca
    18301 gcagaaacgg tgcctgttga ttgccgagac ggagccggcc cgctttctgg aaagattctt
    18361 tgaaaatgtc accccgtcgc agacgtcgtc catgctgctg agcctgaaga atccccggtc
    18421 gcctttgaaa gagaaaatca tccagaagtt ccgtgccctc atggacaacg acgcccggga
    18481 gatacgctgg gttcccgggg ccatggaact gctcgcggcg ctgaagaaga aatacaccct
    18541 cgtggtggtt tccaatctct gggcctatca gaaggaattc ctctcccaca agctcgccgt
    18601 gggcgattgt ttcgaccgct gtctcttttc ctgcgacctg ggtatggaca aaaacgatat
    18661 ccttcggcaa ctgggcgaaa tcctttccgc ggatacccgg gacatggttt atgtgggacg
    18721 ttcctacgag tacgatatcg tccccgccgt caacgccgat atcagcgcga tccggatcac
    18781 ctatgaaaac aacatgatct ttccggaaaa gatcacgcgg atgatcgagg aggaactgga
    18841 cggcgtcgcc gcccccattg ccaggaggag ttttgttgcc caagggggcc ggcgggatgt
    18901 cctcctggtg atacccccgt tctacaagct tctggggagc cataacaacc gcctcaacat
    18961 gggggcggcc ttcatctcct cccgtctgga ttccctgcac atccccaacc ggacctacca
    19021 ctgcgattcc gaaccccatg aaaactatgt gacccgctac cagatggtct tcaattccgt
    19081 cgaattctat gaccacctgg ccggaagcga ggccttcccg gcgttcgagg aatatttccg
    19141 cgcccatggc tcagatacgg tgttcgttac ctgcggggat atcctgaacc cctcttttga
    19201 cagcgggaat tgggattcct cccagaaaat cgccaagatc gtccggaaag tgaatccccg
    19261 ggcctatatc gtcgccgtcg gccccgaggt ggggttggcc tccgaggatt tcgacctgat
    19321 cgttcatggc gaggtggaaa gcctcctgcc ggccatcctg gaaaaccatc aacggggccg
    19381 gatcaacggc gggctcctct ccgagcacgg gttgaaaagt ctgccgccct tccggtttga
    19441 gacgatggct acggaaatct ctcccccctc cctggatacg atcctctggc gccggggatg
    19501 cgccgggacc tgcgatttct gccgggtggc ggagatcaac aaaggtaaaa tccgctgccg
    19561 cccccgggcg gcggttatgg aagacatccg gattcggtac gagaactatg gcatccgtaa
    19621 cttctatatc atcgacgcca acttcaccgc cgacaaagcc atggccctgg agttctgccg
    19681 ggaggttacg gaaaaattcc ccggcatcac ctggcgggtg gagagccgct acgataccct
    19741 ggatgtggag ctcctgcggt cgatgaaggc cgcgggatgc acccatctca aactgggctt
    19801 ggaaaacgcc ctcagcgaaa ggcaccaggt caagaccaag cgggtgagcc tcgccggggc
    19861 ggcggaatgg atcaaggtca ttcaggaggt ggggatcaac tgcgtcatct acctcatgct
    19921 ggggggtaaa tggttcaatc gcgcccagta cgaggagatg ttcgcgaaca caaaagccct
    19981 ccgtgccgac ggctataccg taagcctcta caatccctat ccgaacacgc cggcgggcat
    20041 ctcctacgaa gaatggcggc agcggcggtt cacggggagc catctcgaca tccgcctcat
    20101 cgatttctgg aagatcccca tcgaggtcgt cgaggccttc tttaatctgg agatagaaaa
    20161 aggaagagaa gataaaatgg ttcgacgttt catttgattt agttgctaaa tgtaaaaagc
    20221 attttagtat tattgacggc atcgtaaaaa gtccggatgc ttcgttgcgc ttcatccctc
    20281 gtcactgcga cgtacgcaca agtacgcctc attcctcagg attcgcgcgc ctcgcctgcg
    20341 gatcttttta cgatgccgtc ccaaatggaa cattttttga ccttttacga tgtcgttatt
    20401 attgaatgga taagcttaca taattcacaa tcatcgataa tgttttcaaa aagcctaaga
    20461 gaagaaaaat gaagatcaag gcggggattg tcggcctggg ccgcatcgcc tccctctacg
    20521 aggaggacgc ccgggcgaag gtcttttatc cgtgcctgac ccatgccggg tcctacgccc
    20581 gccatccggc ggtggagatg gtttgcgggg ccgatatcga cgcggagcgg cggcggcggt
    20641 tcggcgcgca gtggggcgtg gcgaaattat attccgacta ccggcaaatg ctggcggaaa
    20701 accccctgga catcctgagc gtctgcaccc acccggaaac ccatttggag attctccagg
    20761 cggccaggaa atccgttccc gtgatcttct gcgaaaaacc ctttacccgg ggaacggcgg
    20821 agatcgaggc ggtcgcccgc tggacggaac aggacgacgc gaagatcacg atcaacctct
    20881 atcgggagta cgacaagtcc cacctccagg tccgatccct cctgcagcgg ggtgaatacg
    20941 gggccatcca gcgggtgaac tgctatatcg gcaagggact ccggaatatg gggacccatc
    21001 tgctgggcta cctcattcat accctggggg cgccggagcg gataaccgtc ctcgaccggc
    21061 gttactcccc cgctgccagg gaatattcct acgatgtcta tctggtgttt ccgggggata
    21121 tcccttgcct cattcagagc tgcgattttg acaaattccg tctcttcgag atggatttcg
    21181 tctgtacgga ggggagaata cagatattgg acgagggact gaagatcgtc gtctgggaag
    21241 cgggggaaaa ccgggcggag acgggggcca gggagctgcg gcgggtccgg cgggggatca
    21301 ggagcaccat cggttatgcc ctgggcggag ccgtggcgca gctggtgcgt ctggccggcg
    21361 cgcccgaact catcccggcc gtatctcccc aaaggtatct ggtcatccaa agggtaatcg
    21421 aagaaataga aagacaggga ggtccgggga agtgcacgca caattagccc tgctgggggg
    21481 taaaccgctg cggacgaaag gctatcccgt ccatgccacg acgggggaag aggaaaagaa
    21541 ggcggtcctg gccgtcttgg aaagggcgat cctttccgag ttcgagggga cgaacaatca
    21601 atacttcctg gggggtgagc aggtaaaggc cctggaagga gagtgggcgg agaagttcgg
    21661 cgtccggtat gccgtgagct tcaactccgc cacgtcggcc ctgtttgccg ctgtcggcgc
    21721 ctgcgcggtg gggccgggag acgaggtgtt gatcacgcct ttcaccatga gcgccacggc
    21781 gacggcgatt ctggccttca acggcattcc cgtttttgcc gacgtggaat tggaccatta
    21841 caatctcgat cccgccgggt tggaggagag gatcaccgcc cggagccggg cgatcttcgt
    21901 cacccatatc ttcggccacc cggcggcgat gggtcccatc atggatctgg ccaaaaagca
    21961 cggcctgcgg gtcatcgaag acgccgccca gtcgcccggg gcgttgtaca acggcaagct
    22021 ctccgggacg atcggagcta tcggcgttta cagtctcaac tgcaacaagg tcattcaatg
    22081 cggcgaaggg ggaatcgccg tgacggacga tgacgatctg gcccagcggc tccgtctgat
    22141 ccgcaaccac ggcgaggcgg tgattgcggc gggcatggcc gtggggagcc tggtcaacat
    22201 gatcggcggc aattaccgca tgacggagat cgaggcggcg atcgccaggg aacaattgcg
    22261 gaaactggac gacctcaccc aaaggcggcg ggagctggta tcctacctca acgaaaggct
    22321 gcggggcatc cccggcctgg ggttgcccgg agcgcggccc gattccaccc atgtttatta
    22381 ccgctatgcc ctgaagatag accgggaggt catccccatc gatgcgcccc tgttcgtcaa
    22441 ggccctgaac gccgaaggcc tggactttta tgtgagctat atgaaacccc tctatctcca
    22501 gcccctctac cagcggcgga tcggctacgg cgacaagggc tgcccgtttt cctgcccctt
    22561 ttaccggggg gaggtgagct atgagcgggg gatctgtccc caggcggagg agctggaaaa
    22621 gatggtgatc tccacggaaa tcgtccggcc gccccagacc tttgccgata tggaggaaat
    22681 cgcccggggg gtggagaagg tgctggcgga gaaagatcaa ctactgaaat acgcggcgac
    22741 ggaagggatg tagagcatga aaagggtaaa tgccattatc gaggcgcgga tgggttcttc
    22801 ccgcctgccg ggtaaaacta tgatggagat cctggggaaa ccctccatcg agctgctgat
    22861 ggaaagattg cggcgggcgc ggaggattac ggacatcgtc atggccacga cggtgaacgc
    22921 cgaggacgac gtcatcgccg cgttctgcct cgattacggc gtgaagtgct ttcgcggctc
    22981 ttcggaggac gtcctggccc gggtctatca tgccgcccgg gaacatcgga ccgacgtgat
    23041 cgtggaggtt acgggcgatt gccccctcct ggacccctgg ctcatcgacg cctgcatcga
    23101 cgtgtttctg aaggccgatt acgattacct gagcaatttc attgaacagt cctatcctcc
    23161 cgggatcgat gtgcagattt tcgctttcgc cgctttggag cggatgcatc gcgcggcgaa
    23221 ggaggcgaag ttccgggagc atgtcaccct ctatatcctc aagaatccgg agcttttcaa
    23281 gatgcataac gtctatgccc cgccggagct ccattatccc gactggcacc tcgaactgga
    23341 cgagtatgcc gactatgaat tgatcaaggc gatctacgag catctctacc ccaagaatcc
    23401 cgcctttacc accatggata tcatcggtct cctcaaggaa caccccgagt ggctggagct
    23461 gaaccgccag gtcaagcgga cctgggagca ggcccggggg gaggatgtcg gatccaagtg
    23521 aggcctgtga acagggagac aacatggaaa atgtaaactg caatctctgc ggaagcagtg
    23581 aaaccgaatt ttggggaaaa aaggcgggca tcgatatcgt caagtgccgt aagtgcggac
    23641 tgatatactg caatccccgt ccggacaacg aggagttgca ggccttttac tccgcagact
    23701 atttcgacga cggacactat gccggcgatg agctgcggca gcggatgtac gagatcgaga
    23761 tcgaggagat cctggccaat gtcgcccgcc ggggacgctt tctcgacgtc ggctgtgccg
    23821 tgggcaagtt cctgctgacc ctgccggata ccttcgagaa gtggggaacg gaattttccg
    23881 ccgccgccgc ccggatcggg agggagaaat tcgggttgaa catcatcacc ggccaggtgc
    23941 gcaacatcga actaccggaa aaatatttcg atattgtcca gatgcggggg gtcctggaac
    24001 attcccagaa tccctacgag gatctcctca gcatgcgccg ggcgctgaag gatgagggcg
    24061 tcctgaggat aagtcagctt cccaatatcg gcagcatcag cgcggtgctc ttccggacca
    24121 agttcaatca ggtcaagccc ggcgagcatc tctattattt ctcgccggag acgatcgggg
    24181 ccatgctgaa gaaggctggt ttcagcatta cgagcattaa ttatccctat ctcgaaacgc
    24241 cctatgccgc tttggcccgc gatattatcg agctggtgaa atgtattttc caggatcggg
    24301 aaacgcccgc ctttttcggt aacatgatga tcgtctatgc aaaaaaggat ttgtagggac
    24361 ctataggaat ggaaaaaaaa ttctcacgtc aggacagctc caaacaaacg gaacagttca
    24421 gttaccgcgt caatgtcaac gatatcctca gcgaggagct cggagaagat ttccgccggt
    24481 atcgggagcg gttcgaggcg gcggcggcct tcgccgtcga accggagtat cccatccata
    24541 tcgatttcga aacgatcttc gggtgcaacc tccggtgcat catgtgcacc catgcccaca
    24601 aggaccggta tccccagcgg cagcggctca tggatatcaa tctgttcaag ggggttatcg
    24661 aggaaggcgt tcagtacggc ttgtcttcca tcggcctgga ccaggaaggg gagcccctgc
    24721 tggtcggcaa tcttctcgat tacatcgcct atgcaaagca aaaaggcatc ctcgatatca
    24781 tgatcaacac caacgccacc ctcctcgatc gggagaagac ggaggcgctg ctccattcgg
    24841 ggttgacgcg gatacacttc tctctcgacg cgattacgga agcaacctac gacaagatcc
    24901 ggatcggttc caatttttcc cgggtaatgg agaatatcct gtatttctgc aaacgtaagc
    24961 aagagctggg caaggtactc cccatcaccc gggtgagttt cgtgaagatg aaggaaaacg
    25021 aacacgaaat ggatgacttt accgcctttt ggacggagcg cgtcgatgcc atcgccatcc
    25081 aggagtacaa ccgcgccttt cccgacgcgg gggaactgaa tcccgccccg ggcgagaaga
    25141 ggatcaagaa tcccgatttc cggtgcaccc agccgtggtt tcgcgtcgtg gtcctcaccg
    25201 atggccgggt cctcccctgt tgcctcctgg gttattcctt gcagatggcc atgggcaatg
    25261 ccggggaaga gtccgtttat caactctgga atgccaagaa aatgaaggcg ttgcggcgga
    25321 tgcaccggga gggcgaatac ttccggaaca agatctgtaa acaatgcgcc ttgaacttcc
    25381 tatagctagc tgatgacgcc atgaaagttc tcatgatgga agtgccgacc cgggaacggg
    25441 acggttccgt gcccttcggg ctactctatg cggcgagcag cgcgcaccgg cgggggcacg
    25501 aggtgcggat cgtcgatctc gtcaaggaag acctccggga tgatgaattg ctccggatga
    25561 tcagggattt cgcccccggg gtgatcggca tggggggcat cacctccagt tacggccggt
    25621 gcaaggaact cgtggccctg atcaaaaagg attttcccgc cgttcccatc gtggccggag
    25681 gggtgatcgc ttccgtggcc gatctcctcc tcacccgggc cggcgtcgat tacgtggtcc
    25741 acggcgaggg ggagatttcc tttcccaatc tcatccaggc cctggaggcc caggccgatg
    25801 tcgctttggt ggcggggatt tctttcctcc gggacggaca gctccggcgg acggcgcatc
    25861 aggagcagat cagggatctc gacgctatcc ccatgcccca ctacggcctg ctgaagatgg
    25921 aaaattatct ggaaacactg gacaaatgga ttgcccacta tctgcggatc gaagacgacg
    25981 tcaacaagtc ggtccggaag gttctggccg ggaagcggta catgtttccc atcattacgg
    26041 cccggggctg cacccataag tgcatcttct gctatcgtca tcaccggggg ctgcggcagc
    26101 acagcgtcgg atatgtgatc gccatgatca ggcatctcca cgaaacttac ggcgtggacg
    26161 tctttcagat aaacgatgaa ttgaccacgg gcagcaaagc gtggatccgt gcgttctgcg
    26221 ccgccctgaa aaaggagggg cttcccatcg ccctgattgt cctgagcgcc cgggtcgata
    26281 acgtggatgc tgaaatcctt tcgttgctga aggaagtcaa ttgcatcatg atcaattacg
    26341 gttatgaaag cggggccaat gacatcctga aggaaatcag gaaaggggtc acccgggaga
    26401 aggccctgga gacgggattg ctgaccaaga aggcgggcat catcaacatc ccggagatca
    26461 tcgtcggctt tccgtcggaa acgcgggcca ccgtggagga aaccatcgat tttctcaaac
    26521 ggttggatac gtggtctttt tccatcaaca cccccatccc ctttcccgag acgccccttt
    26581 gggactacgc cgtaaagcgc gggttgatca aggacaagga agaattcgtg ctcggctacc
    26641 ggcggggggt gtttatcaac cttaccggtt tttccgaccg ggagctcctc agtctggtgg
    26701 cgaaggtgca ctacgatccc tacctgatct ggctgaggcg ccgcggccat taccggctgt
    26761 atctgaagac gctcctggtc cggctgttcg ccgtaaatct ccggcaaata cttcccgaat
    26821 ccgtctttgc gggtttacgg accgtttacc atcgccgcgg cgtgtagatc ctttcgggac
    26881 gatttctcgg gccgatgcct aacaagaagc gaaatctgat tgtcgtcgcc gctcccgaac
    26941 cggcgctccc catggccgga atgaggcccg gccaagccga tgacactgtg gtctcttttt
    27001 ccgtgggggc aaagctggcc ctggaaagag accatataag ctgtctgttt cccgacgacc
    27061 tcgtcgaggt gccggatctg aatgccatcg ggatggaaaa catggccttg gcaaacgaga
    27121 tctgtgccgt catcgacgat gaactgcggg cgcgccttgc ctttttccgc gcccacggca
    27181 tcgatctttt ccggcatgcc tttttcccgg tcaagatttt tttcgacagc ctcattacct
    27241 cctatttgat tttcgcggcg ttgctgaacc acgcggcggg cggtcgtacg acggtaatcg
    27301 ccgctgaata tgccctcgga cggattatcg gcggggaaca ccccctcgtg cccgcgctcg
    27361 ccgaaaaggt ctttatgccg gttcacgagg gcgtttccgt ccgccgggtc ggaaggaata
    27421 aggcgtggcc ggacaggaag gatgactatc gggagtgggc ggggctgctg aagagctgtt
    27481 ggcggagctg gccgcggggg gcgggcaagg cgggcaagca gggcggagtg atccttctcg
    27541 atgaccggta tgacgtcaag gtctgggccg actactgccg cggggagcgg gagttttaca
    27601 aactattttt cctgccgagg ctggtggccc tgaaatccct gcattcaaga cgcgtgcgga
    27661 cggcgggcat cgtcgggggc gacggggatg atcgggacat cgccgcggcg tttcgggcgg
    27721 cggagaagga actggcgggg caccgaattt ttcgcggcca tgcctccctg tgccgttttg
    27781 ccttcgcctg cctggaagga tacctccgct tctccctggg gaaaaatatc accgggttgg
    27841 ggacggcggt caggaagctc ctggccgatg aatcgcctct tctcctctgc acggcggcct
    27901 gccgcatgga tctgaaagac gccttgatca tgggcgtggc gaagtccctg ggggtgcccg
    27961 tggcgaccta ccaggagggg ggcggtgccg gttatctcga ttggcccctg ttcaatctcg
    28021 atgcggaact tgccgattat tttttcgtct atggcgaggg cgtggcggca agcccttttt
    28081 tcgacggccg tccggccgga atcattcccg tcggctcccc gtatcttgcg gaagtggagc
    28141 agcggctgtc aaccgcaaag cgcgatacac gggcccaggg aatctatatc gttctcgaca
    28201 atatcaagac cggtacttgg cagcattatc ccttcaacgg cggcgccttt tttcaggcat
    28261 atcggcatca ggaaaggatt ctccgggccg tgggggatac gggttcaggc cgattcatac
    28321 tgaaaaccgt ccgcggccgg gaaaatctct atgcttccca tatcggcgcc ggcgatcgtc
    28381 tccggatgga gacaaggccc ctggaggagg ttcttggcgg cgccgccgcc ttcatcctcg
    28441 actatccctc caccgttctc caggagtgtc tgctgaccga caagcccatc gccctccttt
    28501 tggacggaaa ccaggtccgc ctcgaggccg cggcgctgga gctgctgcgg cgaaggatcc
    28561 ggatcgcttt cgacgtcgcg gatttcccca tgacgctggc ggccttgcag gcggatgtcc
    28621 aaaacggcaa ccccatgacg aagaatcggg acttcctccg ccgctactcc cgtccggaaa
    28681 aggatatcag taaaatcaac gccatcctgt gggacgccgt ccggagcaaa agatcccttt
    28741 gaacggctac aagttcatcc tcggcaatat cttctggctg agcgcggtgc gcatctttac
    28801 caaggtcgcc gccctgctga ccttgccgat cattacctac tatctttccc cccaggactt
    28861 cggcgtcatc gccatggtca gcgtcgtcca ggcctttctg agcgggattt tcagcatggg
    28921 actcgtttct tactccagcc ggatcatcta tcgttatgaa agaagcgacc ggcgggaatg
    28981 ccgggaatgt ctgggcgtgg tcctctttta tatcttgatg ttttccacgg tgggcgtggt
    29041 tgtttcttcc ctgtttatcg atcggttggt aaatttcttt atcaaggaca taatctttcc
    29101 ccaccggatt tacctgtata tcccggtact gatggcgatg cttttggcaa tctacggttt
    29161 catcagtgat aacatcttga gcttgcagat aaacaagaag atcttctacc tggaaatcgt
    29221 gcagtttgtc ctctttatgc ccttgcagat cgtcggcctg tgttactggg gcttcgatgt
    29281 gtgggatgtc atcgtcctcc aggcggcggt gcaggtggcc ataacggtct atgggctctg
    29341 gctgattcgg gagtggctgt ctttttcctg gcataagctt tcgatcttta aagaggcgat
    29401 gcgttattcc ctccccatgg tgccgttgaa tttcctttcc tgggttcagg accggattga
    29461 taaggtttac ttgaacaata tgatttccct taattcggtg ggcatctaca cggccggggt
    29521 gaatatcgcc acccagtatt catttttgtc ccggccggtg atgaccgcca tcaaaccgga
    29581 gatatcgaaa cgcctcgatg cccaagaccg ggaaatccag tccgacatca aggacgcttt
    29641 tctcatcttc gtgcagtttt ccctgttcct ctaccttgtc atatctcttt tcagcaagga
    29701 agtcgtgcag ttattgatga atgcccgctt tcatgaatgc tatcggatcg tgccgatttt
    29761 tttattgagc attatccttt ctgaggccac cggcattttt caattgaagt ttgtcttccg
    29821 gaatcagacg atctggtttc ccgtgaccct gtttttttcc gccctcctca acgtttactt
    29881 taattacacg ctgatcccga tttttttcgt ctatggcgcc gccctggcca agacattatc
    29941 cgaagtgatc ctatttttca tcaccttgta catatcgcag cggctccacc ggacggatta
    30001 cggattgatc cgcaacctgt ggcccctctt ggcggtgctc gccctggtct attccttgga
    30061 cgccgtcgct ttgtccgcgg catatttatt cttgttgaaa ctttcggtgg ttcttttgta
    30121 tgcattgacg ctggattatc tcctgagcag gatttccagg cgctaccgcg agacaaggaa
    30181 catcgtcacc gcgggcatca gggaacgcat ggcaagatgg aagacataga acgcataacg
    30241 gggaggtggg aaaatgatcg tagcgctttt gttgggaaga aaggggagtg tcggttttcc
    30301 cggcaagaac ctttatcccg tgttgggcag acccttgatg gaatatcccc tcctggcggc
    30361 gaaatacgcc cggaatgtgg accaggtcta tgtatcgacg gatgatgaac ggattatggc
    30421 gctcggggaa aaacacggcg cccggatcat tgtccgccct cccgaacttg cctcccgggc
    30481 cgccctgggg gaacacgcgt acgtccacgg ttaccgcgtc ataaaggaga gcttgggcaa
    30541 ggagggaaaa gaaatcgaat tgatcgtgct gctgttttgc aacgcggcga cgatcctggc
    30601 ggcgaccatc gacgcgggaa tagaaatcct caggaaaaat gaggagtacg attccgcggt
    30661 gaccgtttcc tgctacaata tgtggagccc gctgcgggcg aggaaaatcg gcgcggacgg
    30721 actgttgcat cccttcgttc ctttcgaaac cttcggcgat ccggcgactc tgaactgtga
    30781 ccgcgattcg caaggcgatg tctggtttgc cgatatgggg gtatccatcg ttcggccccg
    30841 gtgcctggat aatctggagg agggactgct gccccagaaa tggatgggga ggaagatctt
    30901 tcccctccag caatggggtg ggtgtgacgt ggacttcgaa tggcagcttg ccggcgtgga
    30961 atactggctg gcgcagcagg ggttttccgc ggctgccacc ccctatgaaa tgagatgacc
    31021 aggatggaaa aaagagttca agcgcaagcc aacgccaatt tttattccct ctggggctcg
    31081 tcggacccgg aggacagaac ggcccgttat gacgaatacc ggcattgctg gatggcatat
    31141 ccgtcccgct tccaggtccg ggaatttccc gtgcacctgg atctcgaagc ctcgagccgc
    31201 tgcaaccttc gctgcacctt ttgcgacaaa ctgcccctgc tttcccggga tcagttgggg
    31261 gatatggact tcgatctata tcggaagatt atcgatgaag gggaggaaag gcagttatgg
    31321 agcgtgaaac tcagttatcg cggcgagccg ctgctcaatc cccgcattgc ggagatggtg
    31381 gcctatgcga agaaaagggg aatcgtggac atctatttca attccaacgg catgctgctt
    31441 accgagaata tggcggataa actgatcgcg gcgggcctgg atcgcatttc catctccgtg
    31501 gaaggtgtgg acccggttcg ttttgaaaag gagcgggtag gcgccaaata tgatcgtatt
    31561 gcgaggaata tagagacatt gatggatctc aaggtcagaa ggaactgttc tcatcccaaa
    31621 gtcagggtcc agacggtctg tctgccctat gtagatctcg atgagtaccg tgattattgg
    31681 cgctaccgct gtgatgaggc cgccgccatc gactacaagg acgccgtgca gcgggatgaa
    31741 aatctgatcg atccgggatg ggcctgcccc cagctttggc aacggatgac cgtggaatgg
    31801 aacggggcga taattccctg taacaacgat gattttcgtc ttctgtcccc gggaaatgta
    31861 cggaaccggg atatatattc atgttggcat gatcccctgg tgcaacgagc gagggaaatg
    31921 cataggcgcg gagagtctca tcttctcgat gcatgccggg gatgtccctg gcgggcgacg
    31981 caggtaaaga aaaatggaga atgagcacgg gcggcaaagg tcatgtctcg ttgtgggcgc
    32041 ttccggcgcc atcgggtccg cggtggtccg ccgtctggcc cggaaagaaa cgaccatggg
    32101 attgcattgc tttaaaaatc ccggttccgt cgcgagcctc tgccgggacc tggggatcga
    32161 tgccaaccgg ggccatattt ttcaggccga cctggggagc gaaaaggcct gttccgggct
    32221 ggtggcggaa tgtcgccaag cgcttggctc cctggacctc ctggccatct gccacggcga
    32281 cgtaggctgg aaggggtggc gtgatctgga gtgggcggat tgggaaacca tcttccgaca
    32341 acattgcctg gccgcttaca ccctggcgac acacgccctc ccgtctatga tcgccgccgg
    32401 cttcggcagg atcgtctatt tgagttccat tgcccctaag tatgccggtt cttcccgatc
    32461 cattcattat gccgccgcca agggggcctt ggagattgcc atgcgcggtc tggcgcgaga
    32521 agtagcgagg accggcgtcc gcatcaacgg ggtgcgggcg ggttttgtca tgactcccca
    32581 gcagcggacg ggtcgcaccg aggaggagca ggaggaacga atcgccaaaa tccccctggg
    32641 acgggcgggc aagaccaatg aaatcgccgc cgcttttgaa tatcttatgt ccgatgacgc
    32701 ggatttcatc acgggcgaga tcattaccgt cgccggaggc gattgaccgt tctattgcag
    32761 ccggccatgg aaacaccatc cccactccag acacagatcc gccggctggt cggcaaattg
    32821 ttggtcaggt cattgcgggc gagcggcgcg caacgggggt acggggaatt gaagcaaagg
    32881 ctcgaggaga ttatccccga catatcgaat cagtattcgt ttcttcaggt aaacccccag
    32941 gacgtctttt tacaggaaaa attgagaaat cagcatgcct ttcagatgga gctgctcttg
    33001 aaggccatgg acatttatgt ccaagacgca aagagggaga ttgtccatgt ggtcgacgtg
    33061 ggcgattctt ccggtgcgca tctccgttat ctaaatgaat tagtgaagga gaagggcatc
    33121 gccattcggt ccatgtccgt gaacctcgat cccgaggcgg taaaaaagat ccaggcgcag
    33181 gggatggcgg cccggcagtg tcgggcggag aaattacacc tgctcaaaga cggcttatcc
    33241 gccgacattt tcatttccta tgaaacgctg gaacacctct ttgaccccat cggtttcctc
    33301 cacgacatcg ctatccattc ttcctgctcc tatttcgtga tcacggttcc ctacgtgcgg
    33361 cgaagccggg tcggtttgca tcatctgcga cgaagatctc cggatcctgt atatgccgaa
    33421 aacacccaca tatttgagtt atgcccggaa gattggggat tgattttcaa ctttgcgggc
    33481 tgggagatcg tttgccagga ttgttatacc cagtatccgt cggccggatt attgaagctc
    33541 actaaatatc tatggcgaag gaaagacttc gacggtttcc tgggggtgat attgacccgg
    33601 cgtccggaaa aggccgccca gtaccgaagc tggtcatgaa gcgatcaggg gggaaatggg
    33661 aacgatactg atcacgacct cgtcgtttgc cgccgggacc ccggaactcc tggacgactt
    33721 gaaagaccgg ggatttccgt acgttttgaa tccttacggg cgggtgctca cgggggatga
    33781 actggtcgga ctccttgatc gacaccggcc cgtgggcatc ctggcgggca cggagaggat
    33841 cgatggcgcc gttctgggcg ccgccaagga tcatctgcgg gtgatttcca gggtcggcgt
    33901 gggctgggac aatgtggatc tcgccgccgc cgccgccttg ggcatcaggg tttaccgcac
    33961 ggagcacgtc cttacccagg ccgtggcgga attgaccttg ggcatgatcc tttcggcctt
    34021 gcgcaatctc gccggtcatg accgccggct ccgggaggcg cgctggcaaa agcagatggg
    34081 ctctttgctg gccgggaagc tgctggggat catcggtttc ggtgccatcg gtcaacgggt
    34141 ggggacgttg gccaggacct tcggcgcgga ggtgatttat tacgatccat gcccccaagc
    34201 cgcggtgacc tgggccggtc cggtatcccg ggcggaactt atggaaaggg ccgagatcat
    34261 ctcgctccat gccggcggcc gggaggtgat catgggggat ggggaactga agtcatgccg
    34321 ccggggggtc atcctgatca atacggcccg ggggacgttg gttgatgaaa gggcactggt
    34381 gcggcattta gcctccggtc aggtcggttt cgcctgtctg gatgtttttg agcaggaacc
    34441 gtatgcgggg cccctgctgg cgttcgacaa tgttttattg acgccccata tcggttccta
    34501 tgcccgggaa gccagggcgg aaatggagtc gcgggccctg gccaaccttt atcgtggttt
    34561 gaaggagacc ggatgcttat gagtaaaaag aacgtcgtcg ttatcggtgg gtccggcttt
    34621 ctcggaagtc atctcgccga tgccttgacc gatcggggat accgcgtaac ggtattcgat
    34681 gtgcgtcctt cgccgtggct gcggcccgaa caggagatga tcgtcggcaa tgtcctggac
    34741 gagggggagg tgcggaaagc gctggcgggg gctcattacg tctatcatct ggccggcatc
    34801 gccgatatcg gcaagtccgt ggaatcgccc cgggaaaccc tcgagaagaa tatcatcggc
    34861 agcgcgaatg tctttgatgc ctgcgtccag gcgggcatcg agcggctcat gtatgcctcc
    34921 acgatttatg tttacagcca gcacggctcg ttttaccggg taagcaaaca gacggtggag
    34981 catatgctcg aggcatacta cgaacagtac gcgctgaaat acacgattct gcgctacgga
    35041 tctctctacg gtcctcgctc ccaggaatgg aacgggatga aacgttatat aacccaggcg
    35101 gtgcgggacg gggtaatcgt ctatcccggg acgggtgagg aaaggcggga gtatattcat
    35161 gtcaaagacg cggcgcaatt gagcattgcc gccctggccg ctgaattcat caatcagcgc
    35221 ttgattctca cggggaccca tgcgttgaac tcccgggaat tgctgagaat gattcaggag
    35281 ataatgaatg aacatgtccg gatagagttc aagaaagaag ggagagatcc cgtccattac
    35341 gacattaccc cctaccgctt cacgccccgg gaggggaaaa aaatggtatc gaatgtgttt
    35401 atcgacatcg gccagggcat cctcgaactt atcgaggagg tttatcggga aatcggttcg
    35461 ggccggcaat gagggactct aaatgaaggt cgtctggctg tcccatgtcc tggcccctga
    35521 tacccccctc tacggcggcg gggacaagat cggttttacg caccccagat cgatggcccg
    35581 gggcgactcc tgcaatacca ctctgctcgg cctatcgagc catgcgggca cccatgtgga
    35641 tgttccccgg cattttctcc cggcgggtgg cgccgttgaa cactacccgc ccgagacatg
    35701 gctgttccac tcgccgctat tgatcgaaat ctcggtgccg ccgggcgagt tgataaggcc
    35761 ggaagatgtc accccgaaat tgcccctgga tcaacccatt gatattctcc tcgtgaagac
    35821 gggtttttcc ctgcggcgac aggaggagat ctactggcgg caggggcccg ggctttcccc
    35881 tgctcttggg gagtatttaa aaaacggcat ttccggtctc cgggcggtgg gtgtggattt
    35941 cctgtctgtt tccagcctgg cgagaaggga ggaggggagg gaggcgcatc gcgccctcct
    36001 ggaaagagaa atcctgatct tcgaagacct ggcgctgggg aatctccaac ctaaagaacg
    36061 gctcacgaag attatcgccc tgccgttgcg cttccagggg ggagacgggg cgccatgttc
    36121 cgtacttggt tgggtggagg ggtagcgctt gccgatcgaa gcgatcattt ttgatttcga
    36181 cggcgtgctc gtggaatctc tggacgtcaa aacggctgcc tttgcccgta tgtatgaaaa
    36241 atacgggccc gagaccgtcg ctcaggtcgt cgattatcac ctgcggcacg gcggcatgtc
    36301 gcgatacgaa aaattccgct actatgaaaa gatcctcctg ggacaccata tcggccccgg
    36361 ggaggagaaa cgattggggg agatgttttc cgcgctggtc gtggaggccg tggtccatgc
    36421 cccctgggtg cccggggcgt atgatttctt gtccgcccat tatcgcacgc aggacctcta
    36481 tgtggcgtcg gggactcccg agccggaaat gatcgcgatc gtcaagcgcc gggaggtgga
    36541 acattttttt gtttccgttc acggctcgcc ggcgtcgaaa gcagaaataa tcacggacat
    36601 ctgtctcgat catcgctatg accgcgattg cgttctcatg gtcggtgatt cccagacgga
    36661 ttatgaaggc gcacggaagg cggggatcct ctttgtggga cgcaaatcag gttcggtctc
    36721 ttttttcccg cccgaggtga cggttcttcc cgatcttacc ggactgcatg attattgtgt
    36781 cgccctataa tcgaccgggg gtttttcttc ttgcgtattg atctttccag ggacggcctt
    36841 ttgacccccg agcaggcgaa acggctcaat gaggtggcgg atcgcacgcg cgttcactac
    36901 aatgatatga tctcccgaat ggcgatcggc aaggaggacg atctcgactg gtgggtttcg
    36961 gggatcgccg gccgcaatac ctatgtctgt tcccttttcg tcgaatgctg ccacgttgtt
    37021 ttgatccggg acctgctggc ggaccaggac atcgcggaga ttgtaacggc atccaggccc
    37081 ctcgccaaga ccatccgttc cgtttgccgg gcccagggtg cggcggtgag ggtcgtcgcg
    37141 aaagagagtt ttttcgaacc cctaaagata ctgtggaaaa gcgcctggga ttgcgggaga
    37201 gacatggccc ggcaaatcta ccgtttttgg ctctgccgga tcaggttccc ttcccggaaa
    37261 ggacctctgc cggcaaactg cacgctcgtc gagcttttcg tcctggacgg aagtttcgct
    37321 gcgggcgatt tccaggaccg ttattatgag aatttcggcg gttgcctgcg ccaggaggag
    37381 gagaaggacc atgtatattt ggcaaacctg gcggtaaggt tcaagaatct tgccgggacg
    37441 gtggagcgcc tgcgagcggc gacgaaacgc ttcctgatcc aggaagaaat tctgcagacc
    37501 agcgactttg tccatgcctg gtcgtatccc ctgcgctgtt ggcccctgtt tccgcggcgg
    37561 gcgctcctct ccgggatcga cgtgaccccc ctgttgcgcc gcgcctggtt gaaacatctc
    37621 ttctccagcc gttccctgga ggccatccta aagttcggtc tcgtcaagag gctcaagaag
    37681 tctggcgcga ccctgcggct cgtcatcgac tggtttgaga accaggatat ggataaggca
    37741 tttaatatgg ccttgcgccg ctacttcccc gagacggcgg ttgtgggcta tcaggggttc
    37801 gacacccaga agtatttctt gtgcgcctat cccacggagg tggagcgccg cagcggcgtt
    37861 ttgccccata gcctggccgt ctgcggtccc gcccttatgg aggagcgccg acagttctgc
    37921 cgcgatctgc cggtggaaac cgcgccggct ttccgttaca tgaatgtgtg gagaggggaa
    37981 acgagaaaaa ctgaagcgcc tttcttccgg gtcttgatca ttctccctct ggaggcgcaa
    38041 agtgccatgg agattctccg cttgctcctg gaagcgatcg aagcggggcc gggagaaaga
    38101 gtccgctttt ttttgaaacc ccatccggcc atgcgcctgg agtcgatata cggagatgtc
    38161 gctcatctga cttcgctgct gaaggttgtc ggcggaaata tctccgcata ctggaatcag
    38221 acggatttga tcatgggcaa cacgtcgagc gccctgctcg aagccgcggc catgggtatt
    38281 cccgtcatcg tcatcggcag ccgccgcggc attaccacca atcccctgcc ggaatcgttg
    38341 tccggggata tctggcgcct ggcatataca aaggaagagc tcctcctgtc cctcgatcat
    38401 ttcatgacgt cggccggaaa ggaccaaaaa cggtttgccg aaataggcgg ccagataagg
    38461 gatagatact ttagtcccgt tacgagaacc gcggtgttaa aattgttgcg ccgctgatga
    38521 ccgctgcgga cagcggaaat cttcacatgg aaaggtcaac ggttgtttgg atatgaagca
    38581 gggatacggt cacaaagata aagcggtaag aatcctgatc accggcggca cggggttgct
    38641 gggggcggct ctccagtatt ttgcccccgg ggatatcgct tgtttttccg tctattaccc
    38701 ggaacgtccc ctgccgctgc cgctgccttt ccccatgcgc gccgccgacg tatcaaaccc
    38761 cgaacaaatg agatcgatat tcgcgtgggc caggccggat ttggtcattc atacggcggc
    38821 catcggcagc gtggattatg ctcaatccca tggcgatgaa accgccatgg tcaacgtggg
    38881 gggaacgaag gtggtggccg agctctgccg gatttttcag gccgccgtcg tttatatttc
    38941 ctctaacgcg gtctttgacg gcgaaaatcc tctgtacgcc gaaacggatc ccgtcaatcc
    39001 cataaaccgc tatggtcgct tgaaggtcga tgcggaagac gtcatccgta acagcggcgt
    39061 cccctgggcc atcgtccggc ccatcctgat gtacggctgg ccttatccgg gggaacggga
    39121 caacccggtg gtttggtggg tccggtcgct ggagagcggc aaggggatca aggtggtgga
    39181 caatgtcttc agcaagcccc tgtccgtatg gtcatgtgcg gaggcggtct ggacgatcgt
    39241 ccggcgcagc gccgccggca tttaccacat cgcggggcgc gaccatatct ctctttatga
    39301 atttgccctc gtgacggcgg aggttttcga tttgaatccc cgcctcatcg agccggtgcc
    39361 ggattcctat ttcccggaga tcgctccccg tccccgggat acctctttcg atacgaccaa
    39421 gatggaaacg gagttggggc tgaaaccgca gggagtgaag gaaggccttg cgtacatgaa
    39481 gagcaggcgg ccgccggacg atgaaaaata aaaagatgtt gatgatctct accgattatt
    39541 acggtattga aagatccatt gcccgggctt tcgccagaaa cggttttgag ccggtattca
    39601 tcaatgcgcg gatggattcg ctggagcggt tgcttagtaa gttcagcagg gtgtttccga
    39661 agacaaaaag aatctgcaac ccttttattc gggccgccct gcaaaaagaa aacaggcaat
    39721 atatttccaa ggccaggata cattcccccg cctttgcctt catcgtcaaa ggcgatgccg
    39781 ttttccccga aaccctaaga tatttaaggg gtgagctcgg catcccctgc ctttcatatc
    39841 agtgggacaa cccctttccc acttatatcg gcaccgccgg tggcaacgat taccgcgacg
    39901 tcaatttccg caacagcctg aacgattacg atcacatctt tattttcgac gatcattata
    39961 cggaggagtt gcgcgaaaaa ggcggcaaaa aagtgagtta tctgcccctg gccgcggatg
    40021 acgaactgtt tcaacacctt gatatgtctg aagatgagcg ggcgaaatgg tccttcgatg
    40081 tctgttttgt cgggatgcct tacgacaaca ggatcgagat cctgaatgcc ttgagcggat
    40141 tccatttggg ggtgtttgga gattactggg aaagatatcg agataaaatg gtgggagact
    40201 attatcgcgg caaggcttca ggtgagacgg tgcggaagat ttacggtgcg tccaaaatag
    40261 tcctgaacat caatcatccc caatcgcggc atggcgtcaa cgcgagaacc tttgaaatcc
    40321 cctgttgtgg agcattcgaa atcgtcaatc atgtccatgg cctcgaagat ttattcgcgg
    40381 tcgggaaaga aattgtctgt tataaaagtg aagaagaact gaagagtttg gtcacctatt
    40441 atctggcgca tgacgaggaa cgggaggcga tcgtcgcgaa aggtcatcag cgggtcatgc
    40501 gggaacacac gtggttcaac cgcgtcaagg acgtcattga attagctgga tgaagaagaa
    40561 catcgccctt ttcatggcct ctgttttggc gacctgcctt gttctcgagg cgctgctgcg
    40621 ggtcgtctat ggcaatcaac ccgtttttat ctatcctcag gtcatgcacg tcccgacgag
    40681 ttatggttac aaattgaaac cgaatcagca gggaacctat accctcgata agccggtcag
    40741 ctctaactcc tatggttttc gggattatga atggcccgtg ccgaagcagc gggggacata
    40801 ccggatcatg tgccttggcg actcccttac gttcgggaac gccgtgcggg gagaagagac
    40861 ctatcccaag gtcctggaac ggaaactgaa agacctgaat gccaactatg aggttatctc
    40921 cgcggcggtc ggcggctggg cgacctttat cgaactcgat tttcttaagg ccgaagggct
    40981 gaaatacgag cccgatgcgg tgatcatcgg tttctacatg aatgacttta ccgtgcgttg
    41041 cgatgcttcc caaaacctga ccaatgaagg ccggtgggat gccagaccgt cgtggcttca
    41101 atggctgccc tacaggtata tttttctcat caaacgttcc gctttgatca cctatctgcg
    41161 ggatcgcatc gcggtggtct gggaaggggg aaacgatttc ggcagccgct tgctgaaaaa
    41221 tcaaatcgac ctggacaatg acgaaagcgt caaggtgacc tacgagtatc tgacggagat
    41281 taaaagcata tgtgatgcaa ggggcatcaa gtgcatcctg gcctccatcc cggcgatcaa
    41341 ttatttctgg tttccgcgcg gttcggtcaa atacatggcg cacctggaga aattctgtgc
    41401 aacaaacggc atagagtttg tcgatctggc cgagggcttc tggaaccgcg aagagaccga
    41461 caaattatat ctgtatccct gggacaacca tctcagtccg gccggacatg aactggcggc
    41521 ggcgcagtta ctttcccggc ttcaaaagac catcggtttt taatgtcccc ccgcgacgga
    41581 catcattgca gacgcatccc atgactccca tccgggtcct aatccacgaa ccatatccgt
    41641 tcggcaagat cgcgggcaac ctgcggaccc aaagttacat aatcaggttg acggaccaaa
    41701 agcgcttcca gttgtcgctg ctgagttctt tcgaaacgga atttacccgg cgaagcgcgg
    41761 gatggggtgt cgaaacattg atcgttccgc cctcacgccg gttgaaccgt tacggcggtg
    41821 aatgtctgcg agacggcttt tgggaaaaga cgctcagcgc ctttgatcta atccgttaca
    41881 attggaactt gagcaagata ttgaaacaga agaaaatcga tctggtttac tgcaactcca
    41941 tccggagcat tcttaccgtc gggctggcgg cccggttcgc cggagtcccc gtttttttgt
    42001 atgtcaaggg agaactgcag aacccacttc tcgatccgct ggggttcatc cttgccagcc
    42061 ggatatgttt cttttgcgcg gccaaccggg acgataaata tccgctgctg gtcgcgttat
    42121 ttgccgggaa gatcggcatc ctgaggatag gaatcgatct ggaggagatc cgggagatcg
    42181 agcgcgggga caagacctcc ttgtcccggg aattggatct ggacggcagt tatgtcaatt
    42241 gcgtgtatgt ggggcagcta tatcctccca agggggtgca cttcctgatc gaggccctgt
    42301 ccatggtcaa ggatgaattt ccccggacgc ggctctatat cttgggagac cacataatcg
    42361 acgcctacaa ggattatcgg gaagaactga ccggtctcat cgagaggcgg ggtttgcggg
    42421 aaatggtttt cttcacgggc tggcgtcctg atgccctggc catagtttca ctcatggata
    42481 tcctcgtgca cccatccctg agcgaaggtt ttggccgggc ggtcctggaa ggcatggctt
    42541 tgggcaaggc ggtcattgcc tcccgggtgg gaggattgcg ggaactgatt gaagacgatg
    42601 taaacgggct gctggtggag ccgggaaatc cgtccatgat cgccgcaggg cttcgccgct
    42661 tgctcgcgga ccggtcgttc cgggaagata tgggggaaga agcgcggcgg cgcgtctttt
    42721 ccgattacga gataaccggc agggtccggg agcttgagga tatatggtcc gggatgacct
    42781 tacggaaatg atgcctgcgt cggtgtcttc tctgtcgatc gttacgggtg cgtccatttg
    42841 gcagttcggt aaatagatca tgtgcggcat ttgcggcgaa tacagtttta ccggcgcaac
    42901 ggtggagcag gcgcatgttg aggcgatggt cgacagcttg cagcaccgcg gtcccgatgc
    42961 ccgggggatt tattgccagg gtgagattgg tttggggcat acccggctgg cgataatcga
    43021 tctgtcgccc gggggcaatc agccgctgtg gtccgcggac cggcgcttct gcatcgtctt
    43081 caacggcgag gtttacaatt accgggaaat cagaggggag ctgattaagc aaggctgccg
    43141 gttcagcggg gcctccgata cggaagtcgt tatccatgcc atccagtgct ggggtatgga
    43201 cagagccttg aagcgtttta tcggcatgtt cgcctttgcc ttgtggaacg aaagcgagcg
    43261 ggaactgatc ctgtgccgcg accgggcggg catcaagccc ctctattact cttgcgacag
    43321 cagccgcctg ctgttcgggt cggagttgaa gtccttatgc gcccatccca ccctggtaaa
    43381 ggaactggac cggacggcct tgggccagca tttcatcaac ggatacatcc tcgccccccg
    43441 cagtatcttc aaagatgtgt acaaacttct gcccggccat tatctggtca tagaccgttt
    43501 tggccggatg acgatgaagg cctactggcg tcttgccgac atagtccggg actcctatca
    43561 gggcagtttc tccgcagcgg cggaacaact ggagtcgctg cttctggatg ccttccgcta
    43621 tcgcctggtc tccgacgtgc ccgtcggcat gttcctttcc ggggggatcg attcttctct
    43681 ggtttcagct atcctccgga aaaaggcaaa tgccgatatc ctcaatataa ccatcggttt
    43741 tcaggagaaa gcctatgacg aagcggccaa ggccagaaag gtttcggaag cgctggatct
    43801 aaaacagatc gtccattatg tctctgagca ggaagtgcag gactcgctgg atagattctg
    43861 cgatatttac gatgaaccat acggggatac ctcggggatt cccacctaca ttctctgccg
    43921 gctggcccga aggcacgtca aggtcgccct gtcggcggac ggcggcgacg agcagttctg
    43981 cggttatgaa gactaccggg cttatgccga ccgttaccgg tggctgggaa gatggcctct
    44041 cgccttgcgc cggattgttt cccaaaccct ggcgcacggg ttgccgtatc gcttgctctt
    44101 atcgctgccg ggcgccatat cccgggcgga gtcttttttc ccccagcatg cggcccgcta
    44161 cgaaaaaatg ctgcgcctgc tgaaagtccg cagcgagggg gacctgttgc gactcatgaa
    44221 tgaaaaggcc tggtccgaag catcgctccc cggctttctc cccggggcgt cgggcgatct
    44281 tttcgcggat acggttctgg atcgagacta tctccacagt taccgcgcgg aaactgtgga
    44341 cgccatgatg cgcacggatt atcaggcctt tctgcgggac gacattctgg tcaaggtgga
    44401 ccgggcgtcc atggccgtct ccctggaatg ccgggacccg tttctggacc atcggattgc
    44461 cgagtttgcc ttcagcctgc cggtggctta tctgtacggc gatggggagc ataagcgcat
    44521 cttgaagaag attctccgga agtgggtcgg cgaagacatt atctccgctc cgaagcgtgg
    44581 ttttatgatc cccctgtatt attggctgcg cggggtctgg gaaccgttcg tgagggaata
    44641 tctgtctccg gaaaaaatca ggaaggtggg gatcctggac gagaaaaagg tgcaacggga
    44701 agtgagcggg ttctatcggc atcacggcgg ccgcgcggaa aagatctgga tgatgatgaa
    44761 ttttcaaatg tgggcggaga agtggtatgt caactgacaa catcggtctc gagctgatca
    44821 gaaagcggaa tgtcggttcc ggtgcggccc tccgcggtta tatactgatg atgatggtac
    44881 gtcccgcggg ggtgtatctg gcgtggctgg cgctgaaact gggattgacg ccccggcagg
    44941 tgaccttcgc ctcttttttg ttttcctggg ttatcgtttt taccgcagca ttcggtggtc
    45001 ccgggggagc ggttgccgcc ttggtcatgg tcctgttttg ggaggccgtc gatgtgacgg
    45061 atggcagcat ggcgagggcg ctgcgcaagc gggacaactt tggcggtttc ctcgactatg
    45121 ccgccggcgt cgttctcatc gcctttctgc ctttcgcgct cggcatggga gtcttcctct
    45181 ggccggaagg gagcctcgat gcattcctcg gctcgatggg aattgccgtt tccgttccgg
    45241 cggtcctttc cctcgccgcc ggcgccggca tcgccgttat ttcgctcctc atgcggctgg
    45301 tgaaccgggt gctgtttatc agattcggag actccttttc ccagtgggac gagaaggacc
    45361 gtaaccaaag gatgtcgatc aaggaactcg tcaacctcct ggtgcggaat ctggaaacca
    45421 ccggcggact tcaggccctc atttttcttt tggcggccgt cttgggatgg ctcgagatcg
    45481 ccctctgtgg ctatctcgta ttctatctcc tgttattgat cctttttatg cggcagacct
    45541 accaaaccta cggtcgctgc gaagactatt atgtagttga ggtgaagcgc cgggacaatt
    45601 aggggccgcg cgccgatcgg tttcgtaatc gcggccgtcc cgccgggggc atacttgtcg
    45661 gggcagtggg cgtcacgagg ccgtctgacc tttgaaagga aggaagattt gaagaaagac
    45721 cataaagacc ataaagacca taaagacaaa aaagagattc tctgcacctt gggaccggct
    45781 tccctgaatg attacaccct ggcgcggctt gccgaactcg gtgtggatct gctgcggatc
    45841 aacctgtccc acaccaagat tgatgattta ccgggaatca tcggtttcat tcaggagcgg
    45901 acatctttgc ccatctgcct ggataccgaa ggggcgcaga taaggaccgg cgacatggcg
    45961 ggggagaccc tgcacctgcc ggagaacgag atgctcttga tccccggcgg cgggggaaga
    46021 agcgatgccc cgcagctcag cttctatcct cccgatatag cctccttctt tgtggaggga
    46081 gacctggtca gcatcgattt caactccgtt ctggcccaga tcgtcgagaa ttccgctccg
    46141 ggcctccggc tgaagatact gatcggcggc cgggtcggtc cgaacaaggc cgtcacgatc
    46201 ctgaatcgcg acgtcctgtt gccggttctg acggagaagg accggcaggc cctggagata
    46261 ggacagcgat acggcctcag gcacgcggcg ctgtcgttcg ccaatcgcgg tgcggatgtg
    46321 gatgtgattc gccgccatgt gggtgaggac acccgtgtca tctcgaaaat cgaatcgacg
    46381 gcaggctttc gtaacctgaa agagattgct gaaaaatcgg atgccctcct catagatcgg
    46441 ggcgatctgt cccggcaggt gcccatcgac atgatcccgc ggatgcaaaa actgattatc
    46501 agcagggcaa aaaaggtggg ggtaaaggtt tacgtggcca ccaacctctt ggagtccatg
    46561 gtcaaatcat caaaccccac ccgggccgaa gtaaacgaca ttttcaatac cctgtgcgac
    46621 ggggcggatg gcttggttct ggccgcggaa acggccatcg ggtcctatcc ggtgcattgt
    46681 gccatgatgg tccgcaagat gatccaacag tatgatgagc acctgaacaa taccttcctt
    46741 ccggatctag acggcaaggg ctccttgctg ctgatcgaac cgcacggcgg cagactggtg
    46801 aatgcctggc aggaaaaccc ggcgccggaa gaaatggccg gattaatccc gctggatgtc
    46861 gatgtatcgg tcctgatgag cgccgagcaa attgccctgg gcaccttttc ccccttgacg
    46921 ggatttatga ccaaggagga aatggagacg gtattgaggg aatatcgcct tcccagcggg
    46981 gtgatatggc ctttaccaat agtattgcag gtacccggga aaatggccgg gaagcttcgt
    47041 gccggggata aggtcgccct gcggctcaaa ggcaccaagg aaatctatgc caccatggac
    47101 atccaacaga tcttcaaacc ggacctggaa cagacggcca gggatacctt tggggtcacg
    47161 gaccgcaatc accccggggt ggcgcttttg taccaacggg gcgaatggtt cttaggggga
    47221 ccggtgcaac tggtccggag attgccttcc ccatacaaac attacgaact cacgccccgg
    47281 caggtacgga cgatcttcgc gcaccggggt tggagcaagg tggtcggctt ccatacccgt
    47341 aacgtaatcc attgcgccca cgagcatatc caaaggctgg ccctggagcg gacccattgc
    47401 gacggcctct tcgcccaccc cgtcgccggt cccaagaaaa agggggatta caacttcgac
    47461 gtcatcatca gaggctatga gctcatgatc caaaaatact atccgcccgg aaaggtcatg
    47521 atcggggcgt ttcagaatta ttcccgctac gccggccccc gcgaggctgt ttttacggcc
    47581 ctgtgtcgaa agaattttgg ttgcagtcat ttcattgtcg gcagggatca taccggggtg
    47641 ggggactatt accaacccgg ggacgtggag cgacttttcg aacgcgtcgg tgatctcggg
    47701 atcgtgccgc tgtttttccc tacccagcat tactgtgagg catgccacga atatgtggaa
    47761 caatgccggc atggcgacga ccatctactc aacatcagcg gtacggccgg ccgaaaaatg
    47821 cttgccaggg gaaagcgccc tccggagtgg ttcatgcgca aggagatttc ggagctcatc
    47881 atccgggagg cgcgggaggg gaaggaagtg ttcgtggcat gaaaacggcg ctcgtgtttt
    47941 ggttcaccgg cctttccggt tcgggaaaga ccaccgtcgc caccgccgtc aaaagccgtt
    48001 tggaggcagc cggcaggacg gtgctcatcc tggacggcga tgatgtccgg aaccggctgc
    48061 acatccagct cggattctca gtggcggata taatcgaaaa caaccggctg atcgcggatc
    48121 tttgtctgga agcgagcggc ggttacgata tcatcctggt tcccatcatc tccccttacc
    48181 aatcatctcg ggcgcatgcc cgttctcttc tgggcgaccg tttttatgag atattcttcg
    48241 ccgccgatct tgacaccgtc atgcggcgcg acgtcaaggg gctatacaaa ctggcccggg
    48301 aaaacaagct gaacaacctc atcggttttt ctccctccaa tatttacgag ccgccggatt
    48361 ctcccgacct gacaatcgat tcccgcatcg agactatcga acagtcgacg gcggcgttgt
    48421 acggttttgt caatgaaaag tggcaggagt tctcttcctg atcatttcat ccttcggcgt
    48481 gatcggtaca tgagatgcag acacgaattt gccggtccgg tcggttaaag cgatagacgg
    48541 gctatgctca ccaatgcgga agtccgttcc tcgctgtcaa ggcgagtggc ccggcggatc
    48601 tggccgaata cggatcccga caacggaata tgcgtcgggc cgttggaaat caatgatttt
    48661 tccgtctgct tctggctgga aaaaaggggt gacgagaggg gaagaggata ctacgtcaag
    48721 atacccaaaa tggatctgca taaaaaggaa gcaaggaata tttgcccctt gtctcaagcg
    48781 gacagggccc tcgggaaaaa tgaatacgac agcctttcgt cgttagcgaa gctcgatcta
    48841 cccgacagcc tggcggtggc ctttgtcaaa ccggttgcct atctggcgga cgataatgcc
    48901 attgtaacgg aaagggtcgc cgggaggccc atattggcat ctttccgtcg ttgcgcattg
    48961 cgcaagcgga tcgatccccg aggtcaagcg gattttatgg atgattgcct cgatcgcttg
    49021 gggaaaacgg ttcgttttta ccatcgcgcc tcggaaaaga gaatggcgac ggtgccgtac
    49081 gagacttttc acaagatcga taaatatttg tgccgactcc agtcccgcgg tcgggaaagg
    49141 aaatttctgg atcaattgga ccgcgtcctg aaagcgatgg gagctcccac cggaggtgcc
    49201 ggagccgctt taaccctcaa agggttggat atcagaaata tccttgccga tggcaacggc
    49261 agatgtttta tccttgaccc cgggaaaagc aaggtggatt ttcccgaggc ggatctggcg
    49321 cgattcattg tgacctgtcg gatcatcttc tggggaaccc ctttgctgtt tctgcgtctc
    49381 agtccgggat atcagtacga aaagaggttt cttgacggtt actatgggga tgagattcct
    49441 caccaaagca ggcttcttgg gcttctaatc atcaaggagc ttcttaaaca ctggtatttg
    49501 gcgaatgtcg ccctggattt gaaaacgtgg cccggtctga tcaagatgat tttgcgaaag
    49561 acgtacatcg atccattcta taaggaacaa ttgcgcaggg aggttgaagg gctcggtgcc
    49621 ggaaagcgat tatgaacggc agacgcgccg ggcttaccgg gatcgttaca aagcggctga
    49681 ataccggcgg caacatacgg atcaacttac ttgggcccgt tttgccacgg cgagagagaa
    49741 gcgcggagtc gcggcgctgc tcaggttatg tgacctgggg ccgcaagaaa tcattctcga
    49801 tattccctgc ggcacgggtg ttttggctga gacgctcgct gaatttccct tccgggtcgt
    49861 ggcggccgat atatcccggg agatgatgcg gctcgctgtt tatgcttaca aaaagggaca
    49921 atgccgagga ttcgtgcagg ccgacattac ctgtccgccc ttcaaaaacg atgcctatgg
    49981 atgcgtcgtc gtgctgggtt tcatgcatcg tgtccctccc ctgatcaggg cggagtcgct
    50041 ccgggcgctt tgccgcctca ccgaaagatt cctcatcctt tcttacagcc tggatagtcc
    50101 cgcccagcgt ttcaagcgcc ggatggtgag gtttgccaac caatgccatc ggtttgcgcc
    50161 ctatccgctg aagagggaag aattgcggcg ggaggtgcat agggaaggat tggccatcag
    50221 gaagataatg ccggtcgttc cgatgctgtc atcggaagtc ctcttgctgc tcgaaaagat
    50281 cccttcttaa ctaaagagat agcgatgtgc ggtattgccg gcatattttc agcattttct
    50341 caaagtaagg aatcgttggc ggtcacggcg cggaaaatgg cggcggcgct caagcatcgc
    50401 ggaccggacg acgagggatg ctggaccgat gccgccggcg ccctggcgtt gggacaccgc
    50461 cggctgtcca tcatcgatct gaccagggat gggcatcagc ccatggtttc ttcgtgcgga
    50521 cgttttgtca tagtatttaa cggggagata tacaactacc tggagctgcg agatgagctc
    50581 cgtaaagagg gatttgcctt tcatggccat tccgatacgg aggtgctgct ggcggcgttg
    50641 agccactggg gaacggaggc ggccctggcg cgagtggcgg gaatgtttgc ctttgccctt
    50701 tgggatgacc gtgaacgccg cctgactctg gtccgggacc gggcggggaa aaagcccctg
    50761 tactatatga ttgcctctcg gaccttgtat tttgcatcgg agatcaaggc cttcaaggtc
    50821 cttgagaacg tcgatttatc cgtggccccg gaagccatat atcattatct gacgttcggt
    50881 tatgtgccgg cgccgggaac ggtgtaccgg ggtatagaag aaattcccgc cggccattat
    50941 gtggtattca acagcgattt gtccgccgcc tcccggccat actgggtcct gtcccgatca
    51001 aacagacggg aacggatctc tttcgccgct gccgtcgacg aggccgaggt gctgctgaag
    51061 gaggcggtga ggatcaggtt gcgggccgac gtgcccgtgg gatgtttcct ctcgggaggc
    51121 gtggacagcg ggttgctgac ggccgtcgcc gccctgcaaa tggacaggaa attacagacc
    51181 ttcacggtag ccatcgagga cggcctgttc gacgagggac cgggagcggc gctggtggca
    51241 agacaatacg gaacggaaca tcacgttctc accatcaaac ctgatatccg ggaactctta
    51301 ccgcaactga tccaggctta tgatgaacca tttgccgatg cctcggccgt gccgtcgttg
    51361 tgcatcgccg ccgaggcgag aaaatacgtc aaggtggtca tcaacggaga agggggagat
    51421 gagctctttt gcgggtaccg ccgccatctg gccatgaagt tttacgatca attcggtgcg
    51481 ctcttccggt tgattccgga ggcaatcttt gccttggcca accgcattct ccctccgccc
    51541 ggggattttc gcaccgcata tgccttcgcc caccgttatc tgcggggttt ggggaaaact
    51601 ccttacgatc gttacattgc ctggtgcgtg gatggcttcg atgagcggga gaaaggggac
    51661 ctctatcggt gccgccccct tatccgggaa tcttcccccc aggtgctggc ggcaaaagcg
    51721 gccgatcatg catccgacgc ccccttcgat cagttcatgg cctgggattt ctttctgaac
    51781 atgcatgacg acatgctggt caagatggat atcgccacga tggctcacgg cctggaaggt
    51841 cgaaatcctt tcctggatca ccgcctgatc gaatgggcat tcagcctgcc cagggacatc
    51901 ctgctggccg acctccgcac caaaccggtc ctgagatcgc tggcaaagag gtatttgccc
    51961 gcggagattt ccctggcccc gaagcggggc tttgaaatcc ccctgatcaa atggctgcgg
    52021 gaagatctgc gggatatggt ttacgatgcc tgccttacca gagacggtat tgtcctggat
    52081 ctgttcgacc ggcggtatgt aaacgatctt ttagacgaaa aattgcccct tgatcccgac
    52141 cggtggtcaa agcgggtatg gatattgttt atgctggcca tgtggggaaa catgaatgaa
    52201 aatcgccttc ttttgtcctg acgacctttc caccgtaatt tttgccaaga gtttcctgaa
    52261 gttcatcgcg ggcatggatc atatcgaagc atatacgatc agtccggtat tgatgaatta
    52321 catcgaccag ctcgataagc ttgccatcag gcactacccc gtgcgattga acagatatgt
    52381 cgatccttgg tcggatttgg actgtttccg gtcggtatat aatatttgcc gccgggagaa
    52441 atttgatgtc ttgataaatt ttaccaccaa gccgaatgtc ttgggggcga ttgccgcccg
    52501 actggcaggg gtggagaaga tcgtctgtgc cgtgagggga ctggggggac ctttcctgcc
    52561 gcagtctgac ctcaagggcc acctgctgca gggaattgtt aaaagcctgt atcgtttggc
    52621 ctgccggatg agtgacatcg tctggttcac caatcagggg gatatggatt tcttcttgaa
    52681 gaccgggctg gtgggagacg aaaaggtcgt aaagacaacc aatgccctcg atattgtcga
    52741 gtattcacaa aactgtattg acggggatga actggacaga ctcagacagg agctgtccct
    52801 gcggggggat gaaaagcttg tggtcatggt gggccggttg atctggtcga agggtatccg
    52861 ggagtttatc gaaaccgcgg catcattggt ggacgaacga ccggacatca aattcgtttt
    52921 gattgccccg gaagagcggg ggcatcccga cgaggtgccg gcagcgtttg ttcaggaaaa
    52981 aggtaaaatt gacaatttct tctggctggg attccggaaa gacgtcaaga atatctatgc
    53041 cctcagcgat cttgccgtgc ttccctctta ctacaacgaa ggggggtatc cccgggccct
    53101 ccttgaaccc atggcctttg gcaagccggt aatcactacc gatctgcccc aatgccgggg
    53161 accggtggaa cacggccgca acggctatct catccccccc cgggacacgc gcgcgctgaa
    53221 ggaagctatt ttacggatat tccatgatga tcgaaaaagg gagaggatgg gggaatattc
    53281 ccggataaaa atcgagcggg agtttgatga ccgccgggtg gtgaaaacga tcctaaataa
    53341 tgtcggggca atatgaaggc atccggttca tcttttcaca ccgtcacgga aaaacccggc
    53401 cttaaggcca cccgggaaca gttggaacgt atctatcacc gctatcgctt tgccgctgac
    53461 tatgcccggg cggccagcgt cctggaggtg gcctgcggcg ccggcatcgg gttgggctac
    53521 ctggcgtgtt ccgcgaggcg ggtagtgggg ggcgacatcg acgaggtaaa tctggagatt
    53581 gcgaaaggaa tttatcgtga ctgggccagg ggtgaagaat ctgcggcgac tccggagata
    53641 atctggctgg atgcccatga gttgacattt cccgacggca gctttgacct cctgctctta
    53701 ttcgaggcaa tctattatct cgccaagccg gaacttttcg tcacggaggc aaaacgggtg
    53761 ctgaaccgca acggccggtt gattatcggt accgtcaata aagactgggc ggatttccac
    53821 ccctcgcctt atacacaccg ctatttttcc gttccggagc tctacgagct tttgaggaga
    53881 gatttttcgt ccgttgttct gtatggagga ttttccatcc ccgaaccaag ccgcctgcaa
    53941 atggttgtct cggcgttaaa acggatcgcc gtccgtctga atctcatccc cggcagtctg
    54001 gccgcccgta cctatctcaa gcgtcttttt ctgggaccgc tccgggcatt gccggcgcaa
    54061 ctgcaggagg ggatgaccga ctatgaaccg ccggttgcca tttccgctgt ccggcctgat
    54121 cgcgatcaca agataatcta tgccgtggcg accaacggac gaacatgatt aaacgcggct
    54181 tcgacattat cgtctcctcc ctgctccttg tggtgttcag ccctttgatg tgtctggtcg
    54241 cggtgatgat caagggggaa gatagcgggc cggtgttcta tcggggcgcg aggacgggga
    54301 aaaacggccg gccgttccgg atatacaaat ttaggaccat ggtggtggat gcggagaagc
    54361 gcggggcctc ttcgacggcc gccgacgacc accgtataac ccgcaccggc agattcatcc
    54421 gcaagtacaa gatcgatgaa ctgccgcaac tgataaatgt ttttcggggt gaaatgagca
    54481 tcgtcggacc gcgtccggaa gtaaaacact atacggatat gtttaccggg gaagagcttg
    54541 gtatcctttc catccgtccc gggatcaccg attgggcctc catctggaac gccgacgagg
    54601 gggcggtcct ggccggggcc gcggatccgg agcaggcata tatggagttg atcagaccga
    54661 caaagttgaa attacagatt aaatatgtta gggagcgatc tttctggatg gacctgaaga
    54721 tcatcttcct gaccctgcgg gcggttgttc aaccggaaag ccaggcggtg cgggaaatga
    54781 gaaggggcct gagatgacga tttcaataag tattacggag gagatgtgaa atattcgcgt
    54841 gaactcttga aagattttta ccgtcggatg ctgcggattc gcctctgtga ggagaccttt
    54901 gtggcgccga tcctcagcgg cgaaattcgc tgcccctgcc atctgtacag cggagaggaa
    54961 ggggtcgccg tgggtgtctg cgcggccctg accgatgggg attatgtctt cgggaaccac
    55021 cgctcccacg ggcattatct ggccaagggc ggttccatgg agaagatggc ggcggagatc
    55081 ttctgccggg aggagggatg ttcccgggga cgcgggggat ccatgcacct catcgccccg
    55141 gaagtgggca tgctgggggc ggcgccgatc gttgcgggga cgatctccct ggccctggga
    55201 gcggccctgg cgtcggtcat caggagggac ggccgtgtgg ccgtgacgtt tttcggtgac
    55261 ggggccacgg gggagggggt gctttacgaa agcatgaact ttgctgccct gaagaagctg
    55321 ccgatccttt tcgtttgtga aaacaattac tatgccaccc acatgccggt ccgggaatgt
    55381 cgagtggatc gggatatctt tgaaatcgcg gcacccttcg gtatcgaaag ccggcaggtg
    55441 gaaggcaacg acgtgctggc cgtttacgac gcggcaaccg cggcggcgga aaagtgccgt
    55501 cgtggcgcag gtcccgtgtt tctggagttt ttgacctatc gttatcgagg ccatgtcggc
    55561 ccggatgaca atatccaggg ggagcatacc gatatccgtc ccccggagga aatagacatc
    55621 tggatgaaaa aggatccgct gatactgctg cgcgattatc ttctggcggg ggggcttttt
    55681 tcttccgggg aattggacgg aataagggcg gaagttgaag cggaggtaga agaggcgttc
    55741 gtttttgcca gagccggcgc ccatcccaag acagaggagc tggaaagata tgtcttcgca
    55801 tagaatcctg agttacagcc tggcgataaa tgaagccctc catcagacga tggcggcgga
    55861 ccccgcggta tttctcatcg gccagggggt gaaaagcccc tggtatgtgg gcaacaccgc
    55921 tcagggcttg cgggagcgct tcggcgacga acgggtcatc gacaccccgg tatccgaaaa
    55981 cgccatgacc ggcgccgcgg tcggggcggc cctggcgggg atgcggcccg tcgtcgtcca
    56041 tccccgtatg gatttcatgt tttatgcctt cgatcccatc atcaacgaag cggcgaactg
    56101 gtattacatg agcggcggca gcctgcccgt acccgttgtc ttctggggga ttctcaaccg
    56161 gggcggcgaa caggcggccc agcactccca ggccctgcac ggcatgttcg cccaggcgcc
    56221 ggggttgaag gtggtcatgc ccgccacccc ctatgacgcc aagggattga tggtggcggc
    56281 gatccgggag tcgaatccgg tggtcttcat cgacgaccgc tggctgtatc ggcttgagga
    56341 accggtgccg gaagagatct ataccgtgga gatcggccgg gccagaattg tccgggaggg
    56401 gacggatgta accgtggcgg cttcgtcgtt tatggttcag gaggccctcc aggcatcgga
    56461 actgttggcc gaggccggga tcgcggcgga aataatcgat ctccggacga taaagcccct
    56521 ggatggagaa accctgctgg cctcggtaaa gaaaaccggc cggctcgtcg ttgccgacgg
    56581 cggctggcgg tcctttggcg tggcggcgga aatttccgcc ctcgcggcgg agagggcctt
    56641 cggggccctg cgggcacccg tggcacgggt caccctgccc gattgtccgg cccccgccag
    56701 caccgccctg gagaatgcct attaccgtaa cagcacggac atcatccagg ccgttcggag
    56761 tatcctctga taccgtcatc gtttctgacg gggaagcaat tagtgctgtg aatctttgca
    56821 ttattccttc ttgtcggaga ttctgtaaag gtcccaaccg ataaaaaacg gcaagaggca
    56881 tgaggttatg gaatggatag acaagtgaag ttcgtggagc cggcgaagct ctacgggatg
    56941 atcaaggaag agctggacgc cgcttattat gaagtcatga gcaagggcga tctgatcgac
    57001 cgggggcaat tgaaacggtt cgaggaaaat ctcgcggcct ttgtggggac taagtacgcc
    57061 gtgggtctga acagcgggta tgacgccctc cacatgtccc tccgggcggc ggggatcggc
    57121 gccggcgacg cggtgatcgt tcccgcccat acctttgtgg ccacggcttc ggcggtggtc
    57181 aatgtcggcg cccggccggt cctggtggat gtggggaagg atttcaacat cgactgcgac
    57241 cgggtggagg aactcctggc gacggcggac cgggatggcg aaggacggat caaggccata
    57301 attcccgtcc atctcagcgg ctacatggcc gatatggcgc gggtcatgga actggcggag
    57361 cgataccgcc tcgtggtcat cgaggacgcc tgccagagcc tgggggccgg tatggaaagg
    57421 acgaatacag tccacagaca acaacaggca ggaggagaca ggcaggagga aacaggggcg
    57481 catcacgaag cgaggcggag aaggaagaaa ttggcaaaac agagcgggga cctttttggc
    57541 gaagcaacag gtgcggtggc gatcggtgcg gcagctccga aggcgacggt cactgggtcg
    57601 gtgtccccgg cgccggaaaa aatcagcgac ggatggggcc gggcgggttc ctggggcctg
    57661 acgggctgct ggagtttcta tcccttcaag atcctcggcg gttacggcga cggcggggcc
    57721 atcaccacca acaatcccga gatcgccctg tttgccaccc ggatgcgcta caacggtgaa
    57781 gaccggcaga ccggggaata ccacggccac ggttttacct gcctcctgga caacctgcag
    57841 gcggcctttc tcgacgtgaa gctgcgctat ctcccccaat ggataatccg gcgccaggcc
    57901 atcgccgcga ggtaccgcgc cgccctgtcc gatctttccg atctcctcct gccccattac
    57961 gacgatcccc gccgggatca tgtctatcag aactatacgg tgcgctcccg gcaggggaac
    58021 gatttttccg agtacctcaa gtcccggggt gtcgaggtgc tgacccagtt ccgcaagcct
    58081 tattaccgtc acgaggccct gaagctggag gatcgggatt ttcccgaaac ggaggccttg
    58141 agccgggagg tctgctccct gcccatgaac gtggagattg ccgacgacga ggtggattac
    58201 gtcatcacgg tggtccggtc attttacggg aagacttgaa aggccaataa gcgaggttga
    58261 atgaatttaa gattgagtga acgccacgcc tgggttctcc agtcggagat ccgcaatatg
    58321 tccatcgaat gcgatcgcgt cggcgggatc aacctctccc agggggtctg cgatacggag
    58381 gttcctccgg tggttcgcca gggcgcccgg gaggcgatgg atcaggggat taacacctat
    58441 acccggtacg acggcctggt ggagctgcgc caggccatcg cggacaagca gcgccgtttc
    58501 accggcatgg cggtggaccc cgaaggggag atcgtcgtca gtgccggcgc gacgggggcc
    58561 ctgtactgca cctgcctggc ccttttgaat cccggcgatg aggtcatcat attcgaaccc
    58621 ttttacgggt atcacatcag cacgctggtg gcaacggggg cgacgccggt ctatgtcccc
    58681 ctgtgccccc cggactggtc ctttcagcgg gaatccctgg agcgggcatg gacttcccgc
    58741 acccgggcca tcatcgtcaa tacgcccgcc aacccctccg gcaaggtctt taccgccgag
    58801 gagctgcaaa cgatcgccgc ctttgccgtc caccacgatc tgtttgtctt taccgacgag
    58861 atctacgagc attttctcta cgacggctgc cgccatctgc cgccggccct cctgccgggg
    58921 ctgggggaaa ggaccatcac gatttccggg ctgtcgaaga ccttcagtat caccggctgg
    58981 cgcgtcggct atgccctggg cgatgcccgc tggcttcaga ccatcggtca tttcaacgat
    59041 ctggtttatg tctgcgcgcc ggcgcccctg cagatcgggg tggcccgggg tctcaacgcc
    59101 ctgggcgacg attattacga aggcttggcc cggcagtatc tccataaacg ggatatgatc
    59161 tgccgggccc tgaccgatgc cggcctgccc cccctggtcc cgcaaggggc ctactatgtc
    59221 ctggcggata tttcccggct ccccggggga aacagcaagg aacgggccat gcatctcctg
    59281 cgggagaccg gcgtggccgc cgttcccggc agcgccttct atcatgaagg cggcggcgag
    59341 gacctggccc gtttctgctt cgccaaagac gaccccgtcc tcgcggaagc ggcggcccgg
    59401 ctccggacct tgaaatgaag aagctcatcc aaaacaagca tttttattac atgcttctca
    59461 tcgatgccgc cctcgtggcg gcatcctata tctgtgccta tctcctgcgg tacgaagggc
    59521 agttgccgga cttcgaatgg cggcggatca tccatatcct gccctatctc gtggccatga
    59581 agctgctctg cttcaccctt ttcggcctct accgagggat gtggcgctac acgagcctgg
    59641 aagacatgaa aaacgtcctc aaggccacgg tggtttcctc tctgatcctc gttttggccg
    59701 tcctgtacat ctatcatttt cggggatact cccgggccgc cttcatcatc gactggctcc
    59761 tgaccctcct ttttgtcggc ggcgtccgga tcggcgtccg gctggcgttg acgaacaata
    59821 tcggtacatt ctggctcttg gcgaaaaaag ggaagcaaga caaacgcctc ctcatcatcg
    59881 gtgccggcgc cgcgggggaa aaggccgtcc gggagatatt ggaaaacccc ggtttgaaat
    59941 tgcatcccct gggatttctc gatgacgatc ccatcaaaca gggcaaggcc atccatggcg
    60001 tcgcggtgat aggcaaggtg gaggatatcg gtaccctccc ggaggatttc gacgagatcc
    60061 tcatcgccat tccctcggcc cggggggagc agatgcgccg gatcgtgcgc ttttgtgaag
    60121 gaacgggcaa gcgtttccgg accatgccca gcatcggcga gttgatcgac ggtaaggtgt
    60181 cggtgaaggc cctgcgggag gtccggatcg aggatatcct gggccgggag gaggtgcatc
    60241 tcgaaagaga attgctgcgg gtatcctatg agggtaagcg catcctgatc accggggccg
    60301 ggggatccat cggcagcgaa ctggtccgac aggtctgcca gtttcacccg gccggggtcg
    60361 gtctcctgga tttcagcgaa ttcaacctct tccagatcga gatgacgcag cgccagtatt
    60421 ttccccacat ccccgtcgag acctatctta cggacatccg cgatccggcg gcggtccgga
    60481 taaccatgga gtcctttcgt cccgaggtgg tttttcacgc ggcggcgtac aaacatgtcc
    60541 ctctccagga actgcacccc cgggagacga tttacaacaa cgtcgccggc acccgcaatc
    60601 ttgcctcagc ggccatggcg gcaggggcgg aacgctttgt cctggtatcc acggacaagg
    60661 cggtgcgtcc caccaatgtc atgggggcgg caaagcgggt ggcggagatg cttttggcaa
    60721 cgctgacggg acattcggcg acccgtttcg tttcggtacg gttcggcaac gtcctgggca
    60781 gttccggctc cgtcgtcccg atttttcagc agcagatcag tcagggtaag ccggtgacgg
    60841 ttacccaccc ggaggtgacc cgttatttca tgagcatccc cgaagcggcc cagttgattc
    60901 tccaggccgg ggccatggga cagggcgggg agatcttcat ccttgatatg ggcaaaccgg
    60961 tgaggatcgc cgctatggcc cgggaagtga tccgtctcca cggcttggag ccggagcggg
    61021 acatccctat cgagtacatc ggcctgcgtc ccggcgagaa gcttttcgag gagctgatca
    61081 ccgtgggtga agggataatg cctaccgccc atgaaaagat caaggtgatc caggggaacc
    61141 agggcgatta tcggctcctc gctgacgaaa tcgacgccct gatcgccgtg gccgatacct
    61201 atgacgccgg ggccatcaag gagaaactcc gcggcatcgt ccccgagtac accccgcaat
    61261 gatcatcggc gaaagctttt cgcttccggg caagctccgt aatgtcctcg tcatccagtt
    61321 gggagatatc ggtgacgtcg tctggtctct tcccgccttc caggcggtgt gcgccgccct
    61381 gccccgggcg gaaatatttc ttttagtccg gaaaggcatc gggtctcttt tggaggccga
    61441 tccggccatt acggggattt tcgaggtcga gagatacccg ggaaatattg tggaacgcct
    61501 gatgaggcag atcccttttc tcgtccggat ccgcaaggtc cgcttcgggc tcgttttcga
    61561 tcttcgttcc gacgaacgag gggcctttat ggcccggtgg acgggcgccc cgtaccgggc
    61621 gtcgctgtgg gccgcgaccg ctccctggta tcgcaaccgc ttgtttaccc atctggtcta
    61681 tcccaccaat gaaaacatcc gcgtccgtct gggggccagt gagcaaacgc tccggatcgt
    61741 caggcaattc ggcatcccca ccccggagac gacgccgcgg ctcgctcttt ccccgacggc
    61801 cgtagatcac atgaaggatg cccttgccgc ccgggatata ctggcggagg gcgagggtaa
    61861 aaacggtggt ccccggggat tcgtcaccat cagccctttt tcccgctggg cttataaaga
    61921 gtggcctctg gagcgctggt cggaaatcgc cgcctggctg gggcgggaat acggactgat
    61981 ttccgtcctt gtcggtgcgg cgggggagcg cgcccgggcg gaggaactgg tgaagacaac
    62041 ttccggagtg ttgttcaatc tggcggggga gacaacgctg ggggaactgg cggccctgtt
    62101 gagtttaagc cgcctccacg taggtgtgga cagcgccgcc cctcatatcg ccgccgccgt
    62161 ggggacgccg acgatcacca tctatggtcc ttccgactgg cgggactggg cccccgtggg
    62221 tgataaacac caggttgtca catcggaaat ggattgctcc ccctgccatc ggaaaggatg
    62281 tgagggaagt gggaggagcc gctgtctcga aaatattccg gtcgaaaggg taagggaagc
    62341 cataaaaaaa aagtttttac cgccacgata aacatgagtg tgattgtctc accttatgta
    62401 tggggtacag gcggcgatca gggaagcggt tttgcctctt cgatgagcat gatggggata
    62461 tcgtccctga tttcgtaaag aagccggcag gtttgacaga tcagaccgtc tccagcttcc
    62521 gtgagggaga tctcgccctt gcatttggga caggcgagaa tctcgagcag ttccttacct
    62581 atagccataa gaattttcct ccttttccgt atatttatgt gaaaatatcc acaagtggcc
    62641 ggatattttc gtctttcgtt gtgcccgaca cgggcatggg ggttactaaa aaaaaccttc
    62701 cggggccggt ctattcggtc cccatgagtt acaaccttcc gtcccacatc actttccccg
    62761 ccccagtttt tcctttactc cttcccaaac ttcttcaacc gttatttctt ccatacactc
    62821 ccgggtcgaa catgttttcc ggaagcaggg gctgcaaggt agttccttcc ggatgacggt
    62881 gtggtgcaaa ccgtaggggc ccgtacgccg tggatccgtc gggccgaaca gggcgaccac
    62941 ggctgtcccc accgcggcgg cgatatgcat cggtccggaa tcggtggtca cgaccagggc
    63001 ggcccggcgg taaaggcagg ccaggtcctt cagactcgtc ataccggcaa gattgacgat
    63061 acgtcccgcc ggcagatgtt gcgcaatggc gtcaatgtaa agttgctcgt cgcggctgcc
    63121 ggtgaagacc accgggattc ccagctcccg caagatccgg tcggccaggg cggcgaagag
    63181 atcctcccgc cagagcttcg ttggccacag ggcgacgggg tttatcgcca cgaaggcggc
    63241 ctcttcgtca agatgatttt tcgtcagcag ggtggtgaca ttctgctcgt tatgttcatc
    63301 ccaggggatg agaaaggtgt gcggaccagc caccggcgcc ttgcctgcct ttacggccag
    63361 ataggccgga aagtccagat aacgatccac ggcgtgtttg ctcatgtctt ccgggatttt
    63421 ctcgttgtag aacagaccgc ttccctcctg catgctgtcg tagcccagct tccggatgcc
    63481 gccgctcaat ccgaccagga cggcgctctt gaaaaggccg tgaaagtcga tgaccaggtc
    63541 atagggcctg gatcgtagct cgttgataaa agaaactatt tcctccataa ttatcctgac
    63601 acgcccactt ttcgcctccc tttgccagcg cttgcgatgg gaaacgatga ccaggtccag
    63661 gcagggatgt ccagtgatca gatctgcggc ggccgcttcg atcacccagg tgatgtgggc
    63721 ctggggatag aggcgccgca gggcggccag ggaaggcagg gtgtgaacca catccccgat
    63781 ggcgctcaat ttgacgatca ggatgttcat ggccctgtcc ctgaacattt gccgcggggg
    63841 tgtccattgc tgtttttttg ccaccatgcc cgccggtggg aagccgattg gtccgctttc
    63901 tcccggagag tattttccga gtcccctata aatctctttc cgcaaatacc ataactgccc
    63961 gtagttacaa aaataacaat catcttgttt tgccctgtcc gaggatccag cggacggcgt
    64021 ccagaatgtc cgatgcgata tgatccgggg cggaagagcc cgcctccatg tctttcccgt
    64081 agccggtgcg gacgaggatg cccttcgccc ccgcccgctg tcccgcctcc acgtctttgg
    64141 ccatatcgcc gatcatatag gaacgggaca ggtcgatggc cagctctgac gcggcccgca
    64201 gcagcagccc cggcgccggc ttccggcagg aacaggaccg gcggtatccg ccaatgccct
    64261 ccgtcggatg atgggggcag aaatagaagc cgtcgatgac cgcgccagct tctccaagca
    64321 tccgggacat ctcggcatgc actgctccca cgaacacttc gtcgaacaag ccccggccga
    64381 tgccggactg gttggttatg acgacgactt tcatcccgct gcggttgatg agctgcacgg
    64441 cctcgccggc tccggggagg aggcggagct tgtccaggcg gtccagatag cccacctctt
    64501 cattgatcgt gccgtcccga tccagaaaga ccgcggcgga tgttttcttc ggcatcggca
    64561 tacccggtta tggtgattta tcaagaccct tgttcatgcc gcttgtcacc tcggatttca
    64621 tctgaaaatg cgttcctatg cgcttgactt ccttcaccaa ttaatttcct ccccttcaag
    64681 gggaggatca agatggggat gggtccggca ttttatgaac attaagcttc gctggttcct
    64741 tctcccggcg gcaagacgat gagagacgcg tccttccccc cggcaccggc ggcttccccc
    64801 accgggacac tgaggccgga aacatgttga gccgtgatga tccggttgat cgcggcggtt
    64861 acctcctccc cgccgatcag attcatgcag cggaaatccg tgggacagac ggttttcaga
    64921 caggggctgc aatccaccgg ctttcgaatg atgacattcc tgcggccggg aggagaggtc
    64981 gtcgcaggat ttgtagaacc gaatatggcg actgtcggca cgttcagggc accggcgatg
    65041 tgcatcaatc ccgaatcgtt ggtgaggaag acatggcagc gggcgatgag ggccatgact
    65101 tcccggaccg aggtccggcc ggcgagattc atgcaccgat ccggcatggc cgccgcaatg
    65161 gtgtccgtta cctcccgatc ctgttcgctg ccgaggagga taaacaccgc ctcccggtat
    65221 tgccggtgca atgtcgtggc cacggaggtg aagcgttcca ccggccagcg cttggcgggt
    65281 ccgtaggtgg cccccggggc catgccgata aagagggcat ttccccccgc cggttcgggg
    65341 aggaggctgt cggccaggga cgcggtttcg gcctggaggt caagctgtaa ttcgtcaccg
    65401 gggtcgaggc agcccagggc cttgaccatc tccagatagt aagagatctg atggacccgg
    65461 cggatggccc ggctccggcg gacggcatgg gtcaaaagcc agctccgggc gtcggtgtta
    65521 tatccggccc ggatcgggat tccggcgagg cgggcgatga tgacagcctc gatggcgttc
    65581 tgcagcagga tcgccatggc gaaacaccgt tgacgcagct cggcggcgag gaggagcttg
    65641 cccgccggcc cggcatgtct ccccggttcc tcgaacacga tcaactcatc caccattccc
    65701 gtttcccggt agatgtccgc cacccacggt ttggcgagga gggagatgtt ggcctggggc
    65761 caggtcttac ggatggccgc gacggccggc agcgtcatta cggcgtcgcc gatccagttg
    65821 accccccgga tcaggatcct ctccatgccc tcccggggga gacgtttgtt tccccgcacc
    65881 cgcaacggct cccataccgt tgtgtctttc accctttgac tcccttaatt cctggcggtt
    65941 cttgttccgc tgagttaaat atcgtgaggg aatttataaa aactgcattt atcataactc
    66001 tccagccgat tattccatct ggcattgttt cgtcttccag cgctgatgga gccagaacca
    66061 ctgatcgggg taccggcgga ccatggcttc gacgaccttc gtgaaacgtt gggtattggt
    66121 gaaaagatcc ttttcataat ccccagtatc gtcgatggaa acggcctcct ggatgatgaa
    66181 acgataggtg ccgtcgggca tgcggggcat gaaggcggga atcaccggcg cccccgtccg
    66241 catggccagc acggcgaggc ccacggcggt gcacgccggc cggccgaaga aatccacgaa
    66301 caccccttcc cggaccgaga cgttctggtc gctgaggata cccagggcct tgttttcccc
    66361 gaggagccgc gtgatggtct tccccgagcc gcccttgggg atcacggtgt ttccgttgcg
    66421 ggtccgcacc caggccgtga gattctcgag gatcgggctg tccagggggc ggtagacgat
    66481 gttcatcggc tgccgcatca tgggaaacgc cacggtcatc atctcccagt tgccgaaatg
    66541 ggcgacaatg gagaggaggc ccttaccttt ggcgaaggtg gcgtcgatat gctccagacc
    66601 ttcgaaggtc acccatctgt gcacattgtc ccgatttatg aagggaatct cgaaaaaatc
    66661 cgccgccacc agtcccagat gacggtaggc ccctttggcg atgtccatga cttccttcag
    66721 gtccttttcc ggaaaggccc gccggagatt gtggagggta atgaggcgct gcttggccga
    66781 ggcgtgatag aaaaggcgaa acaggccccg aaacagccac ctgcggagcg tcaggggtat
    66841 cgccttgaac aggaagatga tgatggtcgt ggatttttct ttcttcatct tggcatcagt
    66901 ttgatgaggg cgccgaaaaa ggcctcttct cccggatcga tgcggagccc gacgctcaat
    66961 atgtgaatgg tacgatagaa ttcttggaaa gcggcgagct tcaccccgtc cttttccgtg
    67021 gtgacgatca tctccaccgc ctgctttgtc gcggccccgg ctattttccg gacgtcgcct
    67081 tccttgtaga aataatgatc gggaaaggca aggaatttga tgatccttgc ccccatggcc
    67141 tccaaggtgg cctggaattt ctccggggcc gcgatggcag tgaaggcgag gattttttta
    67201 tcttgcagta tttccggggg aagcgcttcc ccgccgtctc ccgggcacag ccccgtcgct
    67261 tcataggcgg cccggaaaac cggccgggtt gagtcggcgg gcgaatcgtc cgcggatacg
    67321 cgcctcggaa gctcttcgac taccgtcttg acgacgatat ccgcccgatt cagggcggtg
    67381 cgggggtctt cccggagcgg accggccggc aggagacgcc ggtttccaaa ggggttttgt
    67441 ccgtcaatca tcaggatgtc cacgtcgcgg gccaggcggc gatgctgaaa gccgtcgtcc
    67501 aaaaccagga catcggcggc gaatttattg atggccaacc ggccggagac gacccgttcc
    67561 ggtccggtga tcacgggaat gccgggcagg ctcgttgcca tcagtgccgg ttcgtcaccg
    67621 ccttccgccg gggaaaggaa gatctgccgt ccgtcggata ccacgttgcc gctgccggac
    67681 cggcggccgc cgtaaccgcg gctcaggacg gcgggacgga acccccgggc cagcagtccc
    67741 cgggcaacaa ggatggccat gggagtcttt cccgtcccgc ccacggtgat gttgccgatg
    67801 ctgacgaccg gacggggaag gcgttcggcc cggacggcgc cggagtcata gcggcggttt
    67861 ctcagcgccg tcgccgctcc gtaggcccac gaagggggga tcattgccag cagcagggac
    67921 cggcgccagc cgctgggatc acggcggtac catagttcat ggcatatcga gtggatggag
    67981 ttcatcttcg gtgaattgca tgcagtagag tttatagtat tcacctcttt ttgccaggag
    68041 gaattcatgg tttccctcct cgacgatccg cccgttgacc aagacgatga tccggtcggc
    68101 gttgcgaatc gtggagaggc ggtgggcgat gatcagggtt gtccgtccgg ccatgaggtt
    68161 gtccagggcc ttttgcacct cgatctccgc ctccgtgtcc agggacgaag tggcctcgtc
    68221 gaggatcagg atcggggcgt ccttgaggat ggcccgggcg atggagatcc gctggcgctc
    68281 cccgccggag agcttcgttc ccgcctcgcc gatgatcgta tcgtacccct ggggaagctg
    68341 catgatgaag tcgtgggcat gggcggcccg ggcggctccg acgatctcct cctcgctccg
    68401 ctggatgtcc ccgtaggcga tattgtttcg caccgtatcg ttgaagagga tggtctgctg
    68461 ggtcaccata gcgatctgtc ctcggaggga ctccacggtg acgtcccgga tatcttgacc
    68521 gtcgatggcg atcctgcccg tggtaacatc gtagaaacgg gggattagat tcaccagggt
    68581 ggtcttgccg cccccgctca tacccacgaa ggcgacgacc tctccggccc ggatggagag
    68641 gtcgatgtcc ttcaataccg gcgtctcctc ataggcgaag gagacgtttt caatatcgat
    68701 gctccgggtg atcttcggca gggcgacggc atccgggccg tcgccaatct ccggctccat
    68761 gtcgatgatg ctgaagaccc gctgcgcccc ggcgatgccc tgctggatgg tattgttcat
    68821 gttggtgagt cgcttcaccg gttcgtaaag catgatcagg gccgtgagga aggaaaaaaa
    68881 ggtccccggc gtggcgccgc ccttgatgac ctgaaaaccg ccgtagagga tgatggcggc
    68941 gatgccgatg ccgcccagga attccatgaa cgggctcgcg gcggcattga tggagacggc
    69001 cttgatattc agtttgaaga agcgctcgtt ttcttcggaa aagcgtttgt tttcatattc
    69061 ttccatgccg aaggccttga cgatcctcgt cccggagatc gtttcgtgca gcagcgtcgt
    69121 cagcgccccc atggtgatct gggtccgggt ggccacgctg cgcatcttct tgccgaactt
    69181 ggcgatggga tagatgatca gcgggaagat cagtatggcg atgacggcga gtttccagtc
    69241 ccggtaaaag atgacgaaga tcaggccgat cagggtgaac gtatccttga acatagtagt
    69301 cacggcatcg gagacggccc cctggagggt gcccgcatca ttggtgatcc gggacatgag
    69361 gattcccgtg ggatgccggg taaaaaagga cagggattgt ttctggatct gccggtagag
    69421 gctgccgcgc agatccgcga cgacccggag accgatgtag ttcatcagga ccatctgtcc
    69481 gtagctgcac aaccctttgg caagatagat gaaaatcacc accaggggga tccagatgag
    69541 catctgggcg tccttcttca gaaaaatgcc atcgagggcg ggtttgacga ggaaggccag
    69601 ggcggaggtt gacgtcccga ccacgagcat gcacagcatg gccatgagga aacggccgct
    69661 atggggtttg gcaagtttta aaagtctctt gaatacatcc atttaaagca tctccatggc
    69721 caggtgggcg gcccgccgcg aggcccccgg ggcccccagc ttttccctga tgccggccag
    69781 ttcccgtttg atgcggtttt tcctttcgac gtcgttgaga atggccagga tttcctttgc
    69841 catccggggc ggattcacgt cctgctgaat gagttccggg accacggtct tgccggcgat
    69901 gatgttggcc aggccgatat ggtccacatt cacaaagatc cggcccatga gataggtaaa
    69961 cagcgatatc ttgtaaacaa tgatcatggg cacgccgatc agggccgtct ccagggtcgc
    70021 cgttcccgag gctaccatgg cggcgtcgca gacggcaagt acctcgtaga tttcgttgcc
    70081 gattatcttt acattcagtt tttcctggca gggccggatc atctcttcca tgccgtcccg
    70141 gtccagcgtg tcggcaaggg gcagaacaaa ctgggcggtc ggcagttcct gttttatgat
    70201 ccctgcggca gccagcattt ccgggaggag ttttgtcacc tccccgggcc tgcttcccgg
    70261 caggaggccg atgatgcttc cctcctcgcg aagatcgaac cggcggatgg cctcgctacg
    70321 gtggtacttg gtgtttatcc cgtcgaggag aggatggccc acgaattcca ccgccatgcc
    70381 ttcccggcga tacatctccg cctcgaaggg aaggattacc gccatccggt ccacgcattg
    70441 tcgcagttgc ttgatccggc ccctccgcca ggcccatacc tggggactta tgtagtagaa
    70501 taccgggacg ccccgttttt ttgccgctct ggccaggggc atgttgaaat cgggatagtc
    70561 gatgagaata atcagcgccg gcttctcttc ccacagcgac cgcttcagct tcctcatgat
    70621 gcccaggatc atccccagct tggaaaaaac ctcggtgagt cccaccacgg ccatgtctcc
    70681 ggcccgggcc aggcaacgga ccccggcggc ttccatcttc gggccgccga ttccatagaa
    70741 aaccagaccg ggatcactct ccagcatggc ccgcaccaga ttggcgccgt ggagatcccc
    70801 cgaggcctct ccggcgacga tcaggacctt tttctcataa attgccatgg ttacccaaca
    70861 cccgcgaacg acgagaatac tcaagaaaga acgccggatg cattttcatg ctaatcagaa
    70921 atgtgggaag tgtcccgctt ttttgttatt ttccatgctg tatggaaagg aggatctcgt
    70981 ccttggcctt cttaatttcc cgggcgatgt tcagcgccag gtccagggcg ttccgtccat
    71041 cctcaccgga aacgactggg cgggcgccgt tttggacggc ggcgacgaag gaacggattt
    71101 ccgcttcgag tggatcatgc tggcagactt ccaccgcgtt gctgaaaata tccagcctac
    71161 cggaatcact aatctttttg ctaagagaaa cgaggtcccg cttgttgtag tccacggcat
    71221 ggtaaccgtc gatgccgaag aaacgaatct tctgaagggt cttgcccgtg acgcggctgg
    71281 cggtgatatt ggctacgcag ccactggcga atttgagccg gacgttggcg atatccacca
    71341 tttcggagag gaccgacacc cctacggcat cgatggcggc tacgggtgat tttacaaaat
    71401 gcaaaatgac atctaaatca tggatcatga gatcgaggat gacgtccacg tccgtacccc
    71461 gttcgaagaa agggtggagg cgatgggcct cgatgaacag gggttgttgg atgactttgg
    71521 ccagggcgat gacggcagga ttgaaccttt caataaaccc tacctgcagg accgcattat
    71581 tccttccggc ggcggcgata agagcgtccg cttcggcgag agtgacggtg atgggctttt
    71641 ccacgagagc ggacacgccc gcttccagaa acgtaacggc cgtttcgtaa tgggaaaccg
    71701 tgggcacggc gatgctcacg gcgtccaccc gacccagcag gtcgcggtga tcctgaaagg
    71761 cgagacatcc gtattgggcg gcggcccggt gggcccgctc cgccaccaca tcggcgacgg
    71821 cggtgatgcg gcagtccggg atcagggcgt acttctgcag atgatagtta cccagatggc
    71881 cgataccgac cacaccgatg cggagttcgt tcattaccgg cagatccctc gctgagtgtt
    71941 ctgaatgaag tcgatgaatt gccgtacttc cggaatgtcc ggaacgtcgg tccggacctt
    72001 ttccagcgct gtcttcaaaa gcagggaaga gcgaaagata attcggtagg ccagcttcag
    72061 cgcctggatg gtttccggag cgaaatttct ccttttcagg ccgatgagat tcaagccgta
    72121 gagtttggcc atgtttcccg aggccatggt aaagggcggg acgtctttcc caacggcgga
    72181 gcagccgccg ataatacaat gggcgccgat gcgggtgaac tgatggatcc ccgtcattcc
    72241 gccgatgatg gcgttgtctt ccacatggat atggccggcc aggttggcgg cattggccat
    72301 gacgatccgg ttgccgagct tgcagttgtg ggcgatgtga caataggcca tgatgagatt
    72361 gccatccccc atgatcgtga cgccgatatc agcggcggtg gcacgatgga tggtaacgaa
    72421 ttcacggatc gtgttgtgat tgccgatgat cgtccgcgta acctcgtcgc ggaacttgag
    72481 gtcctggggt acggcgccga tggaggcaaa ctgaaagatc cggcatcccg cgccgatgtc
    72541 ggtatgttcg tcgatgacga catgcggtcc gatgacggta ttcttgccga tattgacatc
    72601 cgcggcaacg atggagtaag gaccgattac gaccccttcc tccagattcg ccgccccgga
    72661 gacgacagcg gtgggatgaa tgttcataag tcggttgttt ccccttcctt tttgctgatt
    72721 atttgctcaa gcgcggcgac acgcctgttt agcgctgcta tcttattcct catttccggc
    72781 aatttaggga tagaggaggc cgcccggagc cattcccgat agggctgctg gggggcgccg
    72841 ccgtaaatct gtccggcggg gacgtttttg gagatccccg ctcgagcggc caccatggca
    72901 tgatcgccga tgctgatatg gcccacaatg cccgcctggc cgccgatgag gacgccccgg
    72961 ccgatgcggg tgctgccgga gacgcctacc tgggccacga ttatcgcatg ctcgccgact
    73021 actacattat gggcgatttg gacgagattg tcgatcttga cgcctttctg aatccaggtc
    73081 cggcccaggg tcgcccggtc gatggtggta ttggcgccga tctccacgtc atcgtcgatc
    73141 tggacgatgc cgacctgggg tatcttgcgg ttttcctgcc cggggacggc aaatccgaac
    73201 ccgtcgctgc cgaccacgac tccggcatgg aggatgacgc gctggccgat aatgcagccc
    73261 tcatagatcg tgacgttggg ataaagtatc gattccgcgc ctacggctgc gttgtccccg
    73321 atcacaacgc cgggatggag aacggcccgg gcggcaatta tggccccggc gcctacatag
    73381 acctgggggt agatcgtcgc ttccggatcc acttccgcgc cggtggcgat aattgcctgg
    73441 ggatgaatcc ccggcgccgg tcgttgacgt cggtggaaca gctccagggc ccgaccgaag
    73501 gccgcatagg gatcggtgac gacgaggagg tttttcccgg ggcactcgac acccggtttg
    73561 accagcaccg ccccggcacc ggtagtgata atcttctcct ggtacctggg gttggccaga
    73621 aaggacaggt ctccttcccg ggcctcatcc agtccgttga cccccttgat cagcaccgtg
    73681 ccgtccccga ttacccggcc gtccagatgg gcggccaact cgtttaaagt tatctccacg
    73741 gtttcgctat ttggacttct ttatcttgtt gaattcgtcg atgacccgtt gggtgaggac
    73801 gttgcccttg gcatggtagg cgacggggag ggtgctgagg tcgatgatca aagtatagtt
    73861 ctccttttcc ccgatggcat tcacgatctt gaggatctcc gggacgttct tgcccaggaa
    73921 ttcctggcgc ttgatttgca tctcgtcgtt ggcgtccttg accatatcct gataatcacg
    73981 gaactttttc tggtaggact gctccttgtc ccggagggcc ttttcggtga ggacgggacg
    74041 ctgtttttcc agttcatcct tgagacgctg cagctccttc tctttgttct ggatgttcgc
    74101 gcggttcttg tcgtagttct ttttcaattc ctccatggtg gctttgccga cttgggagtt
    74161 cattaaaact tgctccacat ggataaagcc gatcttttcc gcctgtgatg cggtcggtgc
    74221 gaccaggaag atggaaaaag cgatggccgc ccctatggcc catattgttt ttttcattaa
    74281 taatcctccg tagttaattg atttctacat catcatgcca atcatgaatt cccagcgact
    74341 cgggtcttcg ccctctttgc ggtccaggac atatccccac tccaggcgta acggccccac
    74401 gggggagtac cagcggatgc cgacaccggc ggtctggcgc atgtcgtcca agtgatatcc
    74461 attttcccag gcgttgccgg tatcgtaaaa gaccacaccc ttcatgccgg cgttcttgat
    74521 caggggaaaa acaaaatcag cgttgaaaga aagcatggtt ttaccaccga tcaagtcacc
    74581 ggttaccggg tcgcgggggc cgatgtcccg caaacccctc agactgttta tgccgccgag
    74641 gtaatatcgt tcataaacgg gcagttcctt tccctcgtta ggctggagga aacctatacg
    74701 gccccggatg tcgaacaccg tttccagggg cagggggaaa aaccaggtgg aagtgccggt
    74761 gtatttggtg aaactcaccg taccccccag gagccagccg gcctgaagaa tggtagcggc
    74821 gtttttggag ccggtggtgg ggaagaaatc gtcgtccgtg gtgtcccggg aaatggtgaa
    74881 tcccatgctg ctcgtcgtgg cattgccggc ttgctgtttg atgtaattcg aggcaaaggg
    74941 ggagatattg gtgacatcgg tgaaggccaa ggtataggag acatagccgg tgaccttctc
    75001 ccagagggga tagccaaaga cggcgccgcc gcccttggta cccagatcgt aggtatcgta
    75061 aatccggctg gagttccaga gatcgaactt gctccacagg gggatgtcga agagccaggg
    75121 atccgtaaag gaaatgtcat aataggagga taccgaaccg atgttggctt tcaagctcaa
    75181 aatctgtccc cgcccgaaaa ggttctgctg ggagatctgc gccatcacca tggccttgtc
    75241 cagggcgctg tagccggccc ccatgctcac catgcccgtg ggtttttcct tgagacggat
    75301 atttacatcc gccagggaat cgtcgggacc tttttccgac tggaaatcca cttcttcgaa
    75361 gtacctcatc cgcataagat tcttgtagct gtctttcatc ttggtgctgc tgtagagctc
    75421 gccttccgtc atttggagcg atcttcggat aaccttgtct ctggtcttgg tattgccgat
    75481 gagggatatc ctgttgaagt aaaccagatt tcccttcttt aagtcataat ttatatccac
    75541 attaagggca ttgtcgttca ctctcgttat cggcgtaact tccgcgtagg catagccctc
    75601 gtcgttgcag gcctgggtga gcagttccat gtctttgatg acggcgcccc gatcaaagtg
    75661 ctcctgtttc cggatgttca gtttttccat cagttccgat cgttttacct tcaactcatc
    75721 gccggtgacg tccacccggc ccaccctgaa ccgctggcct tcggtgatgg ggatctttat
    75781 ataaacccct tttttgtcat agctgatgat cccgtctcct acctgggcgg tgatgaaccc
    75841 gttgttcaga tagaaggcat tgagacggtt tatgtcctgc ttcagttccg tctttttcag
    75901 aaggcccgag tcggtgaaga aatagaaaaa gccctgttcc gacgtcttca tgatcttctt
    75961 gagttcattg tcccggaagg aacggttgcc ttcgaaggag atcttcttga tatacagccg
    76021 ggagttttcc ttgatggtaa agatcacccg gatgtctttc tcccgttccc tttctatggc
    76081 gtaagagatc tcggcgttga aatagccctt gtcgtcatac agggccttga tcttttccat
    76141 ggaggtggcg atcttttcct tgctcaatgc ctgacgggtc ttgaaggtca ggaccgcttc
    76201 gatgtctttg gtatccagct ttttgttccc cttgatctgg atatccgtga tgagtccctt
    76261 ttctttcacg atgaaggtga tggttttccc ttccggggtg tcggcgacat cggcggcgac
    76321 atcgtcgaaa tagccaagct tatagatcgc ccggatatct tcgttgagat tggtttccga
    76381 atatattccc cctggggtgc ttttgaggac ctgactgatg gcgctgttct caatcttttt
    76441 gtttccctgg aacccgatgg cggcaatcct ctgggtcgcc gaggtcttga tgaggatgtc
    76501 cccccgcaac tgtccggcaa tggccagaat gctttccggt cccttgccct gggcggaaac
    76561 gggagggaga tacttccccg ccgcgacgtc gaagatccgc atgtccacat tcagggtttc
    76621 ccccagttcc gtgaccgttc ccgataccac atatcccgcc ccgaccgctt tccccacggc
    76681 ggtgatcgct ttatcgtcgg tcttctgccc cgccaggagg ggcagtattt tccccttttc
    76741 gattatccgg acgtttttag cttttcccag ttcccgggcc agattgtcgt aaaaggaatc
    76801 ttgcagaaag gccgccgggc ttttactgtg gaccgtgaag gggattatcg ccaccgctac
    76861 cggctcgggc ggggctgccg ccgccggccc gatccaccac aaaaaagcga tcagcgatcc
    76921 tattaacaaa gaggccgcaa ttttatgtct tcgagatgtc ataagtcctt ccatcacgga
    76981 ggccgacctt tcgttgcaga cggtccgcca ggtttttgtt gtgcgtgacc acgaccaggg
    77041 ttatcttttt ggtgacattc aattccacca gtaggtcttc tatctttttt cctgtttccg
    77101 tgtcaaggtt tcctgttggt tcatcggcca gcaagacatc cggcgccatt atcaacgccc
    77161 gggccacggc gacccgctgc tgttctcccc cggaaagttc gcctgattta tgggtgaagc
    77221 ggtgcgcgag gccgacgctc acgagcaggg attccgcccg ctgctttgcc tcccggtgcg
    77281 agacgccgtg gatcaaggcc ggcatcatgg tgttttccag ggcactgaat tccggcagca
    77341 ggttgtgcaa ctgaaagacg aaccccacct tccggttgcg gaaatcggcc agcttgtctt
    77401 cgtcccaggt aaagatattc acgttatcga agcgcacctc tccggaagta gggcgatcca
    77461 gcgcccccag gatatgaatc agagtgctct ttcccgcccc ggataccccg agcagggcca
    77521 gtgattcccc cttgcggacg gtgaggtcaa gacccttcag cacgttgatg cggttgccgt
    77581 ccttgataaa ggtcttcttc aaatccttga cctgaatcat cagtcgtacc tcaacgcctc
    77641 cgccggatcc atcttcgctg cccgtcggga aggatagatc gtggcgagaa agcaaatcgt
    77701 catggccccg atgacgatca tggccacatc gccgtaattt accttggagg gaagctccga
    77761 gagataatag acgtctcccg gcaggatctt tatccccagg aggcgctcta tggatgccga
    77821 gatggcggcc aggttgaagg cgatggaaag cccggcgacg gtgccgatga tcgtgccgac
    77881 cgccccgatg gtcaatccct ggaaaatgaa tatcttcatg atattccttg cggttgcccc
    77941 catggatttg aggatggcga tgtccttgct cttctccatg accaccatga tcagggtggt
    78001 gatgatgttg aatgccgcca ccaggacaat aagactcagg atgatgaaca taacccgctt
    78061 ttccagccgc agggcggcaa agaggttctt gttcatttcc atccagttgc gcgcccaata
    78121 gggataaccg agcaccttct cgatgttttt cgcgaccacg tcggcctggt acaggtcctt
    78181 gaccttgatt tccagacccg tgacgatccc cggcatattg agaaattcct gggcgtcttt
    78241 caggtcgatg taggccaggg tcgaatcgta ttcataaaaa cccgaatcga agatgccggc
    78301 gacgataaag cgcttcatct tgggaaccat acccatgggg gtcgaaatcc ccaggggcga
    78361 gacgaccgtg atggtgtcgt agaggtaagc ccccagcatc ttcgccagtt ctttaccgat
    78421 caggatgccg gggagttcta tccggtccgg tgggatggtg agggaggtcc tggtccgtgg
    78481 atccaggtgc tccaccttgc cttctttgat cttccccaaa ctgatgactc ggaaggcgct
    78541 ttccggcgcc agcccccgca gaactacccc cgtaacgccg ttttggctct tcagcatggc
    78601 ctggctgtaa atgaacggtg tcgtggccat gacatctccg atggcctcca cctttttcgc
    78661 cacttcccgg tgccccttca tcggtcccgt gtattccatg agcaggacat gggagttcat
    78721 ccccaggatc ttgttcctca ggtccgtttc gaagccgttc atcaccgcca ggacgatgat
    78781 cagggcggcg acgcccaagg cgatgccgcc gatggacagg aaggtgatga tggagacaaa
    78841 gacctgcttt cgcttggcct tgaggtagcg caggctgatg aagagttcga agggcatgga
    78901 ttactgctcg tctctgatcc ggagctgggg aaagaggatg acgtcgcgga tcgaggcgga
    78961 gtccgtgaaa agcattacca gccggtcaat gccgatgcct tcccccgccg tgggcggcat
    79021 ggcgtattcc agggtccgga cgtaatcctc gtccatctcg tgggcctctt catcccccgc
    79081 ctcccgttcc cggagctgca tcaggaaccg ttcccgctgg tcttcgggat cattgagttc
    79141 ggagaaggcg ttggcgatct cccggccgca gatatagagc tcgaagcggt cggtgaagcc
    79201 cggatcccgg gggttccggc gcgaaagggg ggagacctcc accgggtatt gggttacgaa
    79261 ggtgggctgg atgagctctt tttccacctt ctcttcgaag atcatcatga ggatcttacc
    79321 caccaccgca ttctcctccg gctccagacc catttccgcc gcggtggcaa gcaaacgccg
    79381 ccggtcggcc atgatctcgg gggcgatgcc caggtgcgtc tcgacggcct cccggaccga
    79441 gatccgccgc caggggggag acagattaat ctccgttccc tggtatgtaa accggggcgc
    79501 gccgaagacg ttttccgcaa ggaaaaggaa gagttcctcg gtgaatcgca taaggtcttc
    79561 gtaagtggcg taggccaggt agaattccat catggtaaat tccggattgt ggaaggtgga
    79621 gatcccctcg ttgcggaaat tgcgattgat ctcgtacacc ctttccagtc ccccggttac
    79681 gagccgcttg agatagagct ccggggcgat gcggaggtac aaatccatgc ccagggtatt
    79741 gtgaaaggtc ttgaacggct tggcggcggc cccgccggcc cggggctgca tcatcggggt
    79801 ttccacttcg agaaagtcat gaccgtccat aaagtcccgg atcagcttga tgatccggct
    79861 ccgccggtgg aagacttccc gaacgtccgg gttcatgatc aggtcgagat ggcggcggcg
    79921 gtatctcatc tccatgtccg tcaatccgtg ccatttctcc ggcagggaat gcacggcctt
    79981 ggccaggaga cggagccgcc cggcctcgat ggtcaactca ccggttttcg ttttgaatat
    80041 ctttccttcg atatagatga tgtcgcccac gtcgaggcgc cggaagaggt catactcctc
    80101 ctccggcagc gtctttttct ggatgaaacc ctggaggcgg cccttgcggt cctggatgct
    80161 gatgaaggat gccttgccga aatttctgat ggccatgagc cggccggcga gtgaaaatgt
    80221 gtctttgacc tccgccaggg cgtcgttatc cagggcgtca aaacgcgtcc gcaactccgc
    80281 cgaggtatgg cccacccgga cctcgttggg atagagatcg atccccgcgg cccgcagggc
    80341 ggcaatcttt tccttgcgtg tctttatcag ttcgctttct tccatcgaat gttcgcctca
    80401 tcgttaaaaa gggttcgatt tgctatcctt ttttccgtca atagtcaagg atgatttcct
    80461 tgtggcagac atccgaataa tgttgccatc gtggtgaaac ttatgtatgg tcttctgaaa
    80521 acgaagcttt atgttcatta ctggcgggtc tataaacata tataaggagg gcttatgatc
    80581 aataaagtta ttcttgtcgg cagactgggg ggcgacccgg aagttcgcta cacacccgat
    80641 gggttaatga taaccaattt taacctcgcc acggatgagc aaagaaagga caaaaatgga
    80701 gagcggattc aaaagaccga gtggcatcgc atagttacct ttggaaaact cgccgagata
    80761 tgcaatagtt atctaactaa aggccggctg gtctttatcg agggaaggat ccagacccgg
    80821 gcatgggaag acaaggaggg ggtgaaacgt tatactaccg agatcattgc cggcaatatg
    80881 cagatgctgg aagcaaaggg ccagcagaaa ggaccggcgg ctaatgccgc cgaagcgccg
    80941 gcgccgtcct atttcagtcc ggaatccctt cccggtgacg acgtgccttt ttagcggcta
    81001 ccttaaggaa ggtatgtaaa ctaatgcctg ccaggtccga gcctggcagg cattttggta
    81061 ttgatcaggg aagcgggttg gggcggcgat gatttactgc ttgccacctt ccaagaattt
    81121 taatgatata tctttccccg taattccgga cttgatcgta agggcatcgc tgacttgaat
    81181 atctcggcat cccgttgcca tggactttgc ggtgttcccg gtcaggcgcg ggtcatttta
    81241 ttcacccccg ggccgaactg gcatagcgca cccgcgccgc cgccttccgc caaggacccg
    81301 tatgccgctc ccgaggtgtc acttgggttc gatttttttg ttttccgggg tagcgtcgat
    81361 ttcttcgggt tcccgggtcg ccttcttgaa attcttgatg cccttcccga tagccccgcc
    81421 gatttccggg agtttcccgg ccccgaatat tatcaggatg atcaccagaa taacgagcaa
    81481 ttccggcatg ccgataccaa acatatcagg actcctttct ggaggttgag tttgcttccg
    81541 aaacgtcttt aaaacattgt gccggggttg tcaaatcttt cggcactcaa tcggcacgac
    81601 cacccgtcca catgtcctgg ctgccgtgac gtgaaatggc gtgacgacct cacgtcagtt
    81661 tcagatgctt tacctccccg cgtttcccca gatcggggag ctgtttccct tgaaaccgga
    81721 cttgaacccc cccggcattg ccgacagtga tctgaaaggc tttccgggcc gtacgttcga
    81781 tggcctcccc ctttctcagc atcaattctt ccgctttacc gccgtctgag gttatccgaa
    81841 gccaggtttt ctccgtagcc gcgatgctca gcatcaagtc cttgtcagcc gaacccggcg
    81901 gcggagttga cggggtggac ggtcctgccg gcggagaggg ggaaagagtg gttacggcca
    81961 tccgctcctt cggcattgac ggtggaaggg gggagggatg ttgggcctcc cggttgggcg
    82021 gcgccttttg aacgccaggg gggaagggaa cgtgatcagg ggattcggcg ccgtggaaaa
    82081 agattccggc gactataacg accaacagac caagggcaag aaaagaaccg aggaggtatt
    82141 tcttcttacc tttttttacc ggctgttcgt cccgcctctt ttccgtgggg cgacctgttg
    82201 gtttgtgctg aaaggcgaga taagcttcat atctctttag aacgggggcg ccatccacct
    82261 caagatatcg ggtgtagttc ttgatgaaca tgcgggtgta aaccggttcg ggcagtgcat
    82321 tgaatcggcc tgcttcgatg gcttccagat tggtagatgt aatccgcgtt tcttcataga
    82381 catccttaag agagagccct ttggctttgc ggatgccccg gaggtcagga tacatggatt
    82441 ccgtgtcgat ggttgccggt tccatttgcg tagtattttt atctttatcc tggttttcct
    82501 tcattattca atggttacct tatccgattg tttacccctg aggccgccgc aatcaatgcg
    82561 gccagccgat ttatgcgtca atccttgcca ttacttagca tcaatgatac agccgtgcaa
    82621 acaaattgac gatctggttt tgaggcggtt tcgccgtcag tgatcgctgt ccgggcgggg
    82681 tctgctggat ctaaatccgg gtgggtgaga atccccggca aaaggcgtta acgcgtccca
    82741 cttgccgggg tggaaataaa ggttcaactt aaccgaggag atgtttgata atgatgagga
    82801 tgaaacgatg tccggagcag acggacggtg tcgaaggcgc ctaccggtac tttgcgatgg
    82861 cggtagccgc cgaagccggc cggtatctaa gagacaatct aaacaaccgc caccaggttc
    82921 ggttcaaagg ggtgatcgac attgtcaccg aagcggatca acaagcggaa gatatgatcg
    82981 tcggcgccat tcacaggcaa tatccccatc atgatatctt ggcggaagaa tccgcggcgt
    83041 ttacgacggg ttctccatac cggtggatca tcgacccttt ggacggaacg accaactacg
    83101 cccacggata tcctgtattt tgcgtctcca tcgccttgga atgggaagga aattgtatct
    83161 taggggtggt atacaatccc atgctagagg agatgtttgt tgcggaagca ggccggggcg
    83221 cttatctcaa cgatcgatcc ctggtcgtgt cgaaaacggg aatcctttcg gagggtctcc
    83281 ttgccacggg ttttccttac gatatccgct ggagtcagga aaacaacatg aatttctttc
    83341 aggccatggc cgtggaggtc caagccatcc gccgcgccgg ttccgccgct ctggaccttg
    83401 cctatgtggc ggccggacga tttgacggtt tctgggagct caagctccac ccctgggata
    83461 cggccgccgg ctgcctgctg gtccgggaag ccggcgggac cgtcacggat ctgcgggggg
    83521 aggccttcct gcttacttct cctcatgtcg ttgcctcgaa tgggctgatc catgaccaaa
    83581 tcctgtccgt tctacagggg gtggacaaga cctcctgatc tcagttcagg aaggatgcgc
    83641 gcgtcctgac ccaggattgg gattgagacc gtgctcggca ccgatatgcg gtaaggggat
    83701 ctctggggcc ggtttaaaaa ctgtattcca tttccaagat gatcctgtcg ttgcggaccc
    83761 agcggccgta catactgtcc gattgaccgc cgaaaaattc tccggcaagt ttagtgatga
    83821 gaccgtcccg taatttgtat tttgcctcca gtctttgaaa ccgcccctct tctccccggg
    83881 tgagctggaa attacatata aactggaaag acagcttgtt gctgtactcg aaatttatcc
    83941 ggctcaggat gtcgttgcgg cctagacgcg attttttgga actggccacg tagttttcgg
    84001 cgttctggaa tctcgtgatg atctcgttgg catattccac ggtgacatcg atcttttcca
    84061 gccagatctt tttggcaaaa tcgtcgaagg tataggtgaa accggcgaca tagttgaaat
    84121 aatcgtcgtc cttgcggtca tagctgtaat tatagaccaa ctcgccgtgg aactcccagc
    84181 tttcataggt agtggagaag ccggcggcgt aatcatccac cttgactacc tccttgacgc
    84241 gttgaatcaa ggtaattccg gggaacggcc ccggaatcct ttcttcacgg acgacccaga
    84301 agggattggg cccgtggtag aaggaggcga agaagtccca gccgtgaaat cctttcttct
    84361 tgaaacgggc gaaataaccg aaattcgtat ccgtaacctt ggcgacgtcg tctttcatgt
    84421 tgacattgcc cgcatcgtag agatcgaatt ccgtgttgtc cccctgtttg cgttccccca
    84481 tccagcggga agtctcactg ggctgtttct ggggttggta aaccggcagg aaggccaggg
    84541 tcatcgttga agattcggcg acctcccagt ccagcttcgc ctgccagatg ttgagatcct
    84601 tcacgtccag ggggtcgttc aggtcctggg gacgcatccg gtccgagggc aggtagaggg
    84661 ttgacatacc gttggtgata gtcttcttgc ctaccagaaa attgaagtta tcgcggctgt
    84721 agttgaggta gagatcgttg atgtagaaga tccgccgccg tcgatccaga tcctgccaga
    84781 attcgctgaa caccgtgccg gaataggtgt tttcctcgtt tccgtattcc acccacgcgc
    84841 cgatattcaa atcgaggttt ttatatcccg tcttgtcgga tatcttcagc agggtgttcc
    84901 cgaagtcgtt gcggttgtcc gccccctccc ggtccggggg gttctgccaa aaatgcatcc
    84961 cccggaggcg gagactgccg tgaaaattat cgaccagttt cgagaggatt gttgccggtt
    85021 tggtcggcgt ggcggcaggt gtcgcccctt gatcaccctc ttctgagacc ttttccatct
    85081 ctttgaggag atcgtcctgc tgcagatcca tgggcggctg ggccccggcg agagagggac
    85141 agataaaaac aatgatgatc agcaggacaa gggaaccttt gatcaggcag cctttctcca
    85201 taggcagtat tctcccggtt ttttctccgt catgaggaat gaaaggtata agcacctgat
    85261 cgttcccatg cttccgaatg aaaacgatca ggttcccgac atcaggtgtt ttttgttgaa
    85321 agaacgtaat gcaaaccgtg tcccccggga gattattccg tgctattgaa gatgcttccc
    85381 ggagattatg aactgctcgg tgaaggtgcc gtcgctaaga ttaatgttga cctgccggtt
    85441 tctggtggcc gtcaccgtct tgtggttttt ctcgatattg ttcattaggg tctgcttcgg
    85501 gcgcatgacg gtgccggaaa tcgtctcgaa cttatagttg aactgggttt tttggtgctt
    85561 gttacgcttg tcgtagaaat ccgtcttcag ggtggtgagg ttggcctttt cgatccacat
    85621 gatccgtttg gcataaccgc tctcgttctt tttctttgca ttggcgggca gggcttcgat
    85681 tacatagcag tcgtgggcct ttccttcatg gttgaactgt tctgagcgga tgatgttgta
    85741 attgaagttg tccaggtctt ccggctccat gtcttcatag gtaaaatcgg tacccatgaa
    85801 gtaatttttc ttaccggcct tggcgatacg ctgctctttc ttctgcgccg gcaagtagag
    85861 ccactggtca ttgtcgccac cctttttttc ccaagtgcgc agggcggtgc cccggatgtc
    85921 gccagggcta tggaagacca ccagataacg gtgcagatcc ctgcccacct ccttggcgta
    85981 acgaaccacc tggcggtttt ccttggcgcc gctcttttcc accagcatca tgtcttcctt
    86041 gccgatttcc gtctttacct tgtgacgttc cttctgtttc tccatgacct gccgccccgt
    86101 catctgcgcc gatacaccgg cggcactgaa cagaaacaag atcccgaaca cccagataat
    86161 cgcccttttt gccatttccc gcctcctcat aataaaagat cgtgattgtt caacgataat
    86221 gcttcagacc tcttcccttt ccatgttctc aatccgcttc ttgatatcct ccagctcctg
    86281 ccgctccggg atatggagtt ttttcaagat gacggcgaca atcttttcca cccgttcctc
    86341 caattcgtcc gcgaggttgg tgtagtacca aaaggcgctg aaatccgtca ttatcttttc
    86401 cgtctctcct tctatcgact cacgggtctg acgggtcttt tcggtcagga cttccaccgc
    86461 ctttcgcgcc tctgtctgcg cgtccgtttg cgcggattca cccttgtggg tcaaatcttc
    86521 aacgacttcc cggattttgt ccgttgtcgc cagggccatc cccgtgccga tcagtgccgc
    86581 ctttttcgcc atatccagca tggtttgcct ccccttttat tcctcaactc tttttacttt
    86641 ggcccttaaa tatactatgt gctccgttcg tgtcaatgag caattggctt aacaattggc
    86701 tggttttttt ggatttttcc gttgacaggc cgggggtctt tacattatgt tgtcagacat
    86761 gccaattatc ttatcattcc tcggaggtgg gccgcaccga tcatgaagac agcagctacg
    86821 caaggaaaaa cgccgccgat aacagggaag caatcatcgc ctcctttttc cagccgcttc
    86881 gaaagcatcg gcgtcaaggt gcccgagcaa cgcctttcca cccgggacct gatgagtggg
    86941 gtgaagatgt gggggaagtt cgatctggaa cggctcacgg gcatccggga acggcgggta
    87001 tgttcggaaa cggaggattc ctatgtcctt tcccttgacg ccgctcgcga ttgcctgaaa
    87061 cactccgcat accaggccgc ggatctggaa atgatcatct gctgctccat ttccaaatac
    87121 aaggacggca atgcctttgt cttcgagccg cccctgtccc tttacgtcaa ggaagcccta
    87181 ggggcgaata acgccctcaa cttcgatatt gccaacgcct gtgccgggat gctgacgggg
    87241 gtttccatcc tgaatgattt catccgccgc ggggtgatca agcgcggaat ggtcatctcc
    87301 ggggagaata ttacttccat cagccgcaac gctgtacccc gggtcaagac catcttcagc
    87361 gatcaactgg cgtcgcttac cgtcggtgat tgcggcgcgg cggtgattct ggagagatct
    87421 caggtcgggc aggcggaatt gaagatgtcc aatttcatca ccctggcccg gtacagcgat
    87481 ctctgtaccg gctgggcctg tcctcgtgcc cccggggccc agatggtaac caatgcgcgc
    87541 aagattcatc aggtggccat agccgatatg ccgcctattc tggagaaggc cttgaaagaa
    87601 gcgggcctgg agtttggaga tatcgatcat gtcgttcccc atcagacatc catacgggcc
    87661 atacgttcgg gagacaagca tctggcccat tatttcggcg tccgggcgaa aaacatcgtc
    87721 gtcaatctcc aggagtacgg caacaccgct tcgacgtctc acttcctggc cctgtaccgt
    87781 tatctcaacg aaagactttt ccgaccggga gagaaactga tgtttatcgc cctcgcttcc
    87841 ggtctgatca ttggcgtatt aatctttact atggatcatt tggtggaaag atatgggatc
    87901 gaaaattgaa gcggtggctt tgaacacccc cggatttttc cgtcgggggt ctatcgacat
    87961 ggagatcagg gcggcgaaag aatgtctgaa aaaaacccgt ctggatccca atatgctggg
    88021 attgctcgtc aacacggggg tctatcggga tcgtaatatc ggggagccgg ccatagccac
    88081 cctgattcaa aaggaaatcg gggccaacga aatattcgcc ggcggcggca gtaccttttc
    88141 cttcgatttg accaacggtg gctgcgggat gctcaccggc atgatggtcg tcgatggcta
    88201 tctccattcc ggagacatcg actacggcat ggtggtgacc gccgatgccg acccggtgcc
    88261 gggtagaacc gagggcttcc actttacccc ggccgcggcg gcgattctgc tgtcccgcgg
    88321 cacggagggc catgggtttc tccattaccg gacggctacg gaaggccggt ttatcgacga
    88381 cctgaccggg cgcatcgaat ggatggaata taagggcaag atgcgcaaca tcctcgttgt
    88441 gaaacaggcg gaagggtttg ccgcctcttg tgcccggttt gcccgggaga atctcgattc
    88501 ctttttaaag gaattgtccc ttaccatgaa ggacatagac ctggttattc cctcccagag
    88561 tcctcgggga tttctggaag agctcaaaaa gaagagggat ctggaattaa aactggtgga
    88621 tgtgaccgat aagctcgccg atgttcacac cgccggcatc ggttttgccc tggagcgagc
    88681 catgagtacg ggtcgcttcg ccgcggcgaa gaactgcctc tttctgactg tcggcgccgg
    88741 actgacggca tccctggcgc tatacagaaa tcaggcctga cggtagtttc ccggcggcga
    88801 cggagaccgg gatcctgagc cgggtataaa tctatattgt tttttgtcat cgatgatatt
    88861 gtcctgatga gattcacgcc tgccgatatc gacgccgata acataagtct cccctttgat
    88921 attgccccgc atcggcgtct ttatcccttt gccgattatt ggctgaaggc tccggggttt
    88981 atttaccact atcttgatga agggcagggc gatccggtgg tagccgtcca tggcaatccc
    89041 acctggtcct tttattaccg ggagatcgtg aaggccctcc gtcccgatta ccgggtaatc
    89101 gttcccgatc atgtcggttg cggactttcc tcccgcccgt cggagaagga ttttacctat
    89161 actttgacaa gtcacgtgga caatatggaa tccttcctgg attatctgga tcttaagaat
    89221 aacgtaacgc tcatcgttca tgactggggg gggatgatcg gcatggcctg cgcggcccgg
    89281 cgcccggagc ggatcgcccg gatcgtgatc ctcaatacct gggcctttgt cattccaccg
    89341 gacaaaggca tcccctggca gttgtctttc ctccgtcact tcccggtcct gccggctctt
    89401 ttggtccggg gtttgaatgc cttttcttat ctggcaacct tcctgggcgt ggaaaaggac
    89461 atgccggcgg atgtgcgcac gggactcacc gccccttaca atacctggaa aaaccggatc
    89521 gccaccctgc gatttgttca agacatccct ctatatccag gggaccggag ctatccgctc
    89581 acccaatgga cggacgagca cctccatctt ctgggtggga tccccatgat gatctgttgg
    89641 gggcaaaagg acttcgtctt cgatgaggtc gctctggcgg aatggcgacg gcggtttccc
    89701 gaggcggagg tccatgtctt tccccgggga gggcattaca tactcgaaga cgaaggcgag
    89761 acagtcgtag ccctgatcag ggatttttta gagcggcatc cgcttatgac gtaaaaattt
    89821 tactgggccg ccccggccgt acttcgcgat cggggcaacg cttccggatg gaggcgcgga
    89881 cccgtgggaa gggtagagga agtggaagat aaaaccgttg ccaccgcgaa cctcagcgag
    89941 catctcaccc gtgtcgcaca cctcatgccg gataaaaagg cggtgatctg cccggccgcg
    90001 cccggggcac atgattttat ccactggacc ttccgggaac tggaggagga ggcggaccgc
    90061 cttgcccatg gttttcacca agcggggatt gtcaaggggg taaagacgat cctgatggtg
    90121 cggccgtccc tggagctgtt cgccgtcacc ttcgccctct tccgagtcgg ggccgtcccg
    90181 gtgatgatcg atcccggcat gggccggcag aaactcgtgg aaaacctttc ttccataagc
    90241 gccgaggcct ttatcgggat tcctctggcc catatactgc gcgttctctc gccgggaaaa
    90301 ttctccacgg taaaggtaaa aattaccgtt ggtcggcgcc tgttctgggg cggctatacc
    90361 ctggacgatt tccgccgcgg gccctggcgt cccttcacgc ccgtacccgc gcatcccggc
    90421 gaacgggcgg cgatcttctt caccaccggg agcaccggtc cccccaaggg tgtcgtttac
    90481 gaacatggga tgttcgacgc ccaggtgcgc tatctgagta cccatttcgg ttacggtcgg
    90541 gatgaggtgg atctggcgac cttcccgctc ttttctctct tcgacgccgt tttggggatg
    90601 acctccgtca tccccgatat ggaccccacc cggccggccc aggcggaccc ccggaagatc
    90661 atccacgccc tggagaccca caactgcaag agcatgtatg gctccccggc cctgctggag
    90721 aacctggctc gctacggggc cgagacgggc aagaaacttc ccgccgtcaa acggatcata
    90781 accgccggcg ccccggtacg gcccgacctc atggaggccg tgcattgcct gctgcccgcc
    90841 gatgcccgga tccatacccc ttacggcgcc acggaagtgc tgccggtgag cgatatcgac
    90901 agccgggagg tcctgacgga gacccgtaag atttccgaaa gtgggggtgg gacctgcgtc
    90961 ggccggccgc tggcgggtat ggaagtccga atcatcggta taaccgagga acccctgtcc
    91021 cggatccgag aagccgtaat cctcccgccg ggacatattg gggagatcgc cgtcagggga
    91081 cctgtaacga ccaaggaata tttccagaat cccaaggcga ctctgctggc gaagatgcgg
    91141 gacgacgacg gcggtgtctg gcatcggatg ggcgatgtgg ggtatctgga cgacaaggga
    91201 cggctgtggt tctgcggccg gaaggcacat cgggtggaaa cagccggcgg caccctctat
    91261 accatcccct gtgaagccct cttcaaccgc catccccggg taagacgttc ggccctggtg
    91321 ggggtgggcg tctcgggaaa caaacggccg gtcatcatca tcgaactgaa ggccggagac
    91381 agtggtcgtg ataaggaccg cttgacggag gaactcctgg cgattgcccg ggacaacgcc
    91441 atcaccagga tgataaatac cgtgctatac catcacggat tccccgtgga tatccgccac
    91501 aacgccaaga tcttccggga aaagctcacc gtctgggcgg aggaacggct gaaatgacgt
    91561 tgagaacctt cctctttgcc ctttggtgcc cctatgcccg ttaatgccgg agaaataaaa
    91621 ggagactcac cggcaagagc ggcgcgggga tcggtggccc tggtgaccgg cggcggcggg
    91681 tttttgggcg gcgccatcgt ctcccacctt ctggcggcgg gttacgccgt ccggagtttt
    91741 gctcggagtg actatccgga gctgcggcgc cggggagtgg aagtgatccg gggcgacctg
    91801 gcggatcccg aggcggtgcg gcgggcctgc gcggactgtg ccgtcgtctt tcacaacgcc
    91861 gcccgggcgg gggtatgggg ggatttcgac tccttttacc gccccaatgt ggtcggcacg
    91921 aagaatatcc tggcggcctg cggtcgcgcc ggcgtgggcc ggctggtctt taccagctcc
    91981 gccagcgtag tattcggcga tgccgatctg gctggagtcg atgaatcggt tccctatccg
    92041 gaccggcccc gctcccccta tacggccacg aaggcggagg cggaaagaat ggttctggaa
    92101 gccgacaggc ccgccctgag gaccctgtcg ctccgtcccc acctcatctg ggggccggga
    92161 gacacccaga tcattccccg catcctcgcc caggcccggg ccggcaagat cctccgtgtc
    92221 ggtgacggaa agaacatcat cgacaccacc tatatcgaca acgccgccgc cgcccatctc
    92281 cttgccgccc aggccctggc ggagaatccc tgggccgcgg ggcgtcccta tttcatctcc
    92341 aacggcgagc ccgtgaatct ctgggatttt gtcaaccgca ttctggccct ggcggagatc
    92401 ccgccgatcc gtcggggaat ccccaaaacc gtcgcggtcc ttatgggggg ggtcatcgag
    92461 cggctccatc ttgtcctgaa gctgcccggg gaaccccgga tgacccgctt cctcgccgag
    92521 gaactggcta cgacccattg gttcgacatc ggcgccgccc ggcgggaact gtgctacgag
    92581 ccccgcgtct ccatggcgga gggactgcga cgcctgcaaa catggttgca aacccggaac
    92641 tgaaaaaggc cgagaaataa agttttccct attcccggaa cgtcatcttg agctcccaga
    92701 ggccggcgac gtttttcctc tccaggtcga aacctccctg ctcgaaggtg tggagcatgg
    92761 gctggttctc cgagaggacc tccgccgtga aacccaacag cccctgtctt ttggccagat
    92821 aggtcaggta ggctagcagt tccgtcccga tgcccatgcg atggtgggta tcctttacag
    92881 caaaagccac ttccgccgca tgggccgttt cgtcgatgta gtatcggccc acgccgacga
    92941 tctcctccgc cttctcccgg aacatgatgg ccaggatggc catttcaact gtataatcta
    93001 tgattaccag tttctgaagg acctcgtggg gcatgtcctt ccgggccgac atgaagcggc
    93061 ggtagaggct ctgatcggag agggagtaga tgaagtcttt gaggaggggt tcgtcgctga
    93121 ttttgatggg acgaaagagg atgttgatcc ccttcttcgt ggtccggtag gtctccaggt
    93181 gctcggggta ttccccccgg acgccgggga tgaaggcctg gtcccggtag atgagcccca
    93241 gcttcttggc ctcgtcgatg agccaggggc ggaacttggg atgggcgatg ccgatgagtt
    93301 ccatggcccg ttcccggatg ctcttgccgt ggaggtaggc gattccgtac tccgtgacga
    93361 cgtagtggat gtccccccgg gtcagggtga ccccggaacc ttcccggagc atggggacga
    93421 tgcgggatac gacatcgcag gcggcggtgg actggagggt gaggattgac ttgccccccc
    93481 tggacaggac ggccccccgc atgaaatcag cctggccgcc tacgccgctg tgaaagatcc
    93541 ggccgatgga ctccgccgtg gcctgaccgg tcaagtcgat ctccagggcg ctgttgatgg
    93601 ccgtcatccg gtcgtgccgg gcgatgatca aggggttgtt ggtatagtcg atggtccgga
    93661 actcgatggc cgggttgtca tggagataac ggtaggtgtc ctgattgccg aggcagaagg
    93721 tcgttaccgt tttgccggga ttgagggtct ttttggagtt gtcgatgacc cccgtcttca
    93781 tgagttcgat gatcccgtcg ccgatcagtt ccgtgtggac cccgagatgc cgcttgtcct
    93841 tgagattggc caggacggcg ttggggatgc tgccgtagcc cacctggatg gtgtccccgt
    93901 cctccacgag gcgggcgacg tacttgccga tccccatgac gatctccgag ttgacccctt
    93961 tggtgtattc caggaggagc tcgtcataag ggacgatata atcgatgtcc cggatatgga
    94021 tgaaggagtc gccgtggacc cggggcatga aatggtttac ctgggcgacg acgatcttgg
    94081 cctcctcggc ggcggctttg acgatgtcaa cgctgatgcc caggcttaga tagccgtgct
    94141 cgtccgggag ggaggtctgg atgagggcaa tgtcaacccg ggccagcccc cggcggaaca
    94201 actccggcgt ttccgagaga aggacggggg tgtaatcggc gacgcccttg ttgaccgcgt
    94261 cccgggtgct gtcgccgatg aagaaggagt tgtggcggaa gttctgcttg aatttctccg
    94321 tggcatacgg ggccacgccg agggaacgga tatggattac ttcggcgtcg aaaaaggcct
    94381 tggggtaaga ctttacataa cggatcagac cttggaccag atgctggggt tcggcgcagg
    94441 ccgagccgat gaagatggtg tccccccggt ggatgtgact gaagatctcc gcttccggga
    94501 cgaatttatc cggataaagg gtcttccatt tctccaacga cataatctta ttcctcgctc
    94561 gaaagaaata tgttgttccg gggccggcgc cgagggaagg taagatcggg atttgccacc
    94621 gtaagcccat tttcgcataa atggatgaga tgtcaaggtg aaaccatgaa cggggcgggg
    94681 cgaaaccacg gcaccgcctc ctccggaagg ggtcatgatg tcctgcgcct cttcgggaca
    94741 aacgaaggat tgacatggac aggggattta tttataggag ttccgggcca tgaaggcccc
    94801 gccggttctc cgtcccgtgg cgggtaaggc cggaaacaat tgactcttat ggcggtaaca
    94861 taatgaaatc gtttttttat cccaaaagca tggtggtggt cggagcatcg gccacgaaga
    94921 tgaacctggg gcagatcatc ctcctcaaca acaagcagtg cggctatcag ggcagacttt
    94981 acggcgtggg ttcacaggcg ggagaggtgg ccgggatacc cgtgtacacc cagatcgacg
    95041 atctgcccga aatccccgag gtggcgatca tgctcacgcc ggccaggaca attcccgccc
    95101 tcctggaaga ttgcggccgg aaaggcatcc ggcgggtggt tatcgaatcc agcggtttca
    95161 gtgaatacgc ccacggtgac cattccctgg agcaggaggt cctggatgcc gccgccagac
    95221 acgggatcag gttcatcggt cccaactgcg tggggacggt gaatttcgac atcaagatga
    95281 tgatgccctt cgccttcttc aaggctcctc cctccggagg ccggttggcc atgattgccc
    95341 agagtggggg tgtcggcggg acctatctcc atgccctgcc tgagaacgcc atcattcccg
    95401 ggaaatttgt cgccgtcggc aacaaactcc aattggatga ggttgatttt cttgaatatt
    95461 ttctcatgga cgaggaaacg gacgttgtta cattgtacct ggagggtttc agtaggggcc
    95521 ggatgttcca tgatctggcc atgggttccc acaagcccat cattgtacag aaatcaaacc
    95581 ggagcgagat gagcgcaaag atcgcccagt ctcataccac ggccctatct gccggtgatg
    95641 acgtcgtgaa cggcatgttc cgccaggccg ccgttatccg agccgaggac gagggggaat
    95701 tcctcaatgc catcaagatc atccgcctgc ccctgatgaa gggccggcgc gtcgccgtcc
    95761 tgtcccggtc cggcggtcat gccgtgcttt ccgccgacgc ctgcgacaaa tacggctttg
    95821 acatggcgcc cttcccgtca gcctttctaa aaaagctcaa gaccctttac cacacccggg
    95881 tcatcgccca ccagaatccc ctggatttgg gcgagatctt cgattacacg atctttaccg
    95941 gcatcctcga ggaaaccctg aaactggaca acatcgacgg ggttcttttc aatcacctct
    96001 acgcctccgc ctatgaggcg cagatgtccc ggaccttcct cgccggggta gccaaactcg
    96061 tcgatcaata cggaaagccc gtggccgtgg ccatgctctc cgaacccgcg gagcttcttg
    96121 acgtcgcccg gagccagccc tatcccatct tcacctctcc cacacaggcc gccgcggccc
    96181 tcgatatgtc ggcgacctat cacgaacgga agacccggcg ggataaccgg ggcgcaccgc
    96241 cgccgatctc cctggacgag gcgcgcattg ctgccgtccg ggagcgctgc cgccgggaac
    96301 ggcggatccc ctggacggac gaggccctgg aaatctgccg ggcggcggga ctcacgccag
    96361 cgccggcctg gaccgtccgc cgtcccgagg aaggggagac ccttgccgtc cgttttcctg
    96421 tagcggtgaa actcctttcc cgggaggcct cccacaagac cgacgtgggc ggggtgcggc
    96481 tctccgtccg aacgcggagg tccctccgca aggtcctggg ggagatggcg gagcgattcg
    96541 cctccatcgc cgacggattc ctgatccaag aaatggcggg gccgggcgtg gaatgctttg
    96601 tcggcgggcg ccgggacccg gtgttcggtc ccgtggtcat ggtggggctg gggggtatct
    96661 tcattgaggt cttcaaggac acggctatcc gcctggcgcc ggtgaccaag gccgaggcgg
    96721 cggacatggt cgccgagttg aaggcctatc cgctcctcag aggtttccgg gggcgggaag
    96781 ccgccgatat cgcggccctg gaggaggtga tctgccgggt ttccaccctc ctggccgcca
    96841 ccccggaaat cgccgaactg gacctcaacc ccgtgatcgt ccatccagcc ggcaaggggg
    96901 tttccctggt ggatgcccgg atcttctttg cccagcgaca gttagaagac tgatccccgg
    96961 cggccggaga aaagattttc tccggccttg caccggggcg tggcgtcatg tcgaggcaat
    97021 gggaagggct atccgaggcg ggaaacgtcg aggcaagtta gaacgagaag catgcaaatc
    97081 ccaaccgctc ctggagccca cctggtgctc atccatccgc ccgtatccaa gccctgtgag
    97141 ccgccggcgg ggatcgcgcg gctggcggga tatctggagg cgcagggcgt gtcctgtgac
    97201 ctcatcgacg ccaacagcga gggattccgc ttcctcttcg gtccggc
//
LOCUS       DAOSMF010000003        66021 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_547_length_66021_cov_19.206106, whole genome shotgun sequence.
ACCESSION   DAOSMF010000003 DAOSMF010000000
VERSION     DAOSMF010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 66021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 66021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66021
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_547_length_66021_cov_19.206106"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(285..923)
                     /locus_tag="PLT63_01060"
     CDS             complement(285..923)
                     /locus_tag="PLT63_01060"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HOD97340.1"
                     /translation="MKMGRKTKTVAIYARVSTDRQKVDMQLHELRQFVARSGWNIYKE
                     YIDQNYSGGNTKRPAFTAMMEAARKRKFDILLVWKLDRLSRSLKDLIITLDDLGACGI
                     DFVSYDNNLDTSTPTGKLVFQIVGAVAEFEKDIIRERVVAGLAVARSKGKRLGRPPIP
                     QDVYDRAAKMRSEGLSYRSIVKVLGIDEGTIRKKAHKKLHAAKTGSRAAAFN"
     gene            complement(920..2071)
                     /locus_tag="PLT63_01065"
     CDS             complement(920..2071)
                     /locus_tag="PLT63_01065"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD97341.1"
                     /translation="MSNAYERSFVSQLEQRLIAESPRIQVLVGPRQVGKTTGVKQLLA
                     RYPYASHYANADDLLNTDRTWLLEQWQKAVSLGRQTLLVIDEIQKVPNWSDTLKSLWD
                     KDPQSIRVAVLGSSSLQLQKGLTESLAGRFELIKAHHWTFHEQKVAFGYDLDRYLTYG
                     GYPGAVAYEDDFDRWYAYLKDSIVEAVIGKDILLNRRVGNPALFRQAFEILCRYPAQE
                     ISYTKLLGQLQDKGNTDLVKYYIELYSGAFLIHPLEKYSVKNYLTRGSSPKIIVSCPA
                     LYAMNEGPQFLEDREKRGRIFETAIGSQLQQLAGNLYYWREKNLEVDFIYKYRGKIYA
                     IEVKSGRRKSSRGLDAFIKKHPSVNPVIITPEIFPVFSEDPLMFLRKIS"
     gene            2805..3626
                     /locus_tag="PLT63_01070"
     CDS             2805..3626
                     /locus_tag="PLT63_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97342.1"
                     /translation="MSSEIENKKSNEKKLRHRFSSWWHHYPAIERFTLSVAVFTAFLA
                     VFTAFLGVVGFLQWCTLEKTVDTSRIRDRANVYFSTPVFEKYPPTGEAKEYIFKILMA
                     NSGAIPARKLHVRYGVIVAPTSNIDFEPFDRVKWINVNVPMTLGPKQPFLFQLFKIET
                     KLAEFEEMKKGKVVKFIVTEVTYNDDFSSERRQRITQMNVRLNVDGNGDHSFSYLASH
                     NCTDDDCQVREGVATSQTPSPGPFPSDQESPSMRSLRMASVSSSRLPQSAAPKTR"
     gene            3804..8654
                     /locus_tag="PLT63_01075"
     CDS             3804..8654
                     /locus_tag="PLT63_01075"
                     /inference="COORDINATES: protein motif:HMM:NF037202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOD97343.1"
                     /translation="MTFIDILNDIRKKAFSEQDKGKRFERLMRSYLLTDPLYANTLES
                     VWMWSDFPFRNDFSGKDTGIDLVARTTAGDFWAIQCKCYGADAYIDKSGVDSFLSTSG
                     KQFRNESLEKVSFAHRLWIATTSNWSQEASKVLLNQQPPVSRISLSDLSNAPVDWDLL
                     MQDRIGKTSRLTTHKVRKHQQDALDAARDHFVQHDRGKMIMACGTGKTFVSLRLAEQE
                     TGGSGLVLFLVPSISLLGQTLREWSAQANEPLKAICVCSDAEVSKARKRTSDDSADQI
                     EDLALPASTNVASVVYQLESHEQDKRKRGLTVVFSTYQSIDVISKAQRNNGRPFDLII
                     CDEAHRTTGVTLEGDDESAFVKVHDNAFLDARKRLYMTATPRIYGEVAKAKAQEMDAA
                     ICSMDNPDFYGEEFYRIGFGKAVEKLLLSDYKVLILTVHEDSIPAAFQNALAAAENAK
                     EIPADDVMKLAGSINALSKITIGQDTSSLRAIDPDPMRKAVAFCQSIRASKTITNAFN
                     TFKETLYEQYDPSTRLQLVDVSADHVDGTMSALTRDAKLSWVKSSPASGRECRVLCNV
                     RCLSEGVDVPSLDAVLFLSPRNSQVEVVQSVGRVMRTAPGKKYGYIIIPVIIPSGTAP
                     EDALDDNERFKVVWTVLNALRAHDDRFDAIINKINFNKRLPDQIQVIDSAGGSSAGQS
                     WSDEERRQFEAAVDRKSIADLSALCHVIFAKMVEKVGNKRYWEQWARDVAHIAQRHIE
                     QITAFIAQGGPQKSAFDRFMKGLHQNINPSVTPEEAIQMLGQHLITKPVFDALFENYT
                     FVENNPVSKAMQGMLDQLDALTLDKDRAVLQEFYESVKMRTTGIDNGEARQKIVVELY
                     DKFFKTAFPKVVEKLGIVYTPIEVVDFMLQSVAIVLKVEFGRELSDENIHILDPFTGT
                     GTFMTRLIQSGLISNKALDRKYTSELHANEIVLLAYYIASINIENAYHERNLDKPYKP
                     FEGICLTDTFHLGEGESKFDFQDGFVDNTRRVKAQRKAPLRIIIGNPPYSVGQKSAND
                     NAQNQRYPSLESRIEQTYVAASKATNKNSLYDSYIKAFRWASDRLDPQGGIVAFVSNG
                     AWLDGNAMTGFRKTLQQEFSSIYVFNLRGNQRTSGELSRREGGKIFGSGSRTPIAITL
                     LVKNPAAKPPATIRYHDIGDYHTREQKLEIIKSFGSIAAMRDKFKILHPNRHSDWISH
                     RDESFEELIPLAPEKKFDSKSQSVFLTCSNGLKSNRDSWIYNFSQSLLDTNIQTSIEF
                     YNAQAQAFAEARKSNPSIKIENFIQYDSTKFSWNRGSKNDAAKGKQYRFEPESICTAL
                     YRPFCRQYGYFSAPMNDMVYQLPKLFPTTKHENLLICVPGIGVTKDFDCFITDCLPDV
                     QLMANGQCFPLYFYDKNEHVQQESLFDTGEKYIRRDGISDFILNRVRELCGPKATKKN
                     IFYYVYGILHSKQYRAKFAVNLKKSLPRLPLPEDPKAFWELSKAGVELAELHLNYETL
                     PPHKAIMEDSPKKAPSTRVQKMRFDKRGNEEDKTTIIYNEDITLRNIPLEAYDYVVNG
                     KSGIEWVMERYQVTTHKESGIINDPNLWCDEHDNPRYILDLIKRLATLSLETRRIVNA
                     LPVLDL"
     gene            9490..9804
                     /locus_tag="PLT63_01080"
     CDS             9490..9804
                     /locus_tag="PLT63_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97344.1"
                     /translation="MHKRQDVVRHEGGYLVRVFQCIDGFRARYGQWPTKLNLGKLAYE
                     GFYSHLTEFGLGLIKSKINIEIDYTEIDRISAADDSNNRLDYGWYGERPDIDAYQWLR
                     IE"
     gene            complement(10195..10416)
                     /locus_tag="PLT63_01085"
     CDS             complement(10195..10416)
                     /locus_tag="PLT63_01085"
                     /inference="COORDINATES: protein motif:HMM:NF025001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOD97345.1"
                     /translation="MNKNIISIINQKGGVGKTTTSINFSAGLARKGKKTLLIDLDPQA
                     HSTIGLGIEPESFTASITDVLAKKKPIKG"
     gene            complement(10690..10965)
                     /locus_tag="PLT63_01090"
     CDS             complement(10690..10965)
                     /locus_tag="PLT63_01090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOD97346.1"
                     /translation="MPLVKVKRYCQITLPSLIRKKFNIDEGDYLEIEEIDGNMVLKPV
                     KVVQSDLAYFYTKEWQDGEKEADKDIAKGRMLGPFDNVEDFAKAMDS"
     gene            11104..11295
                     /locus_tag="PLT63_01095"
     CDS             11104..11295
                     /locus_tag="PLT63_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97347.1"
                     /translation="MKMKKTLGTMVAALAVVLLTGTFALAEYAAAGVANFPYFQLGCL
                     IIGGMTVISPFVHNFLLKS"
     gene            complement(11321..11605)
                     /locus_tag="PLT63_01100"
     CDS             complement(11321..11605)
                     /locus_tag="PLT63_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97348.1"
                     /translation="MSLKDDLEKASQESIEETDGLLLDEFNSLKQATKRDLDSLRPKV
                     SDPTIYNKMIPIITEATQNNLALAEFQQKLEGLGTGAVKLGKEVAKLLAI"
     gene            complement(11608..12270)
                     /locus_tag="PLT63_01105"
     CDS             complement(11608..12270)
                     /locus_tag="PLT63_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97349.1"
                     /translation="MKKSLFNLIIFLIAFSMFFYGCATIPKETVELSYAIGQDLDAVH
                     TSYANLIHQHFENLRNQTNDFLDKKWTPTYLGNFVKKGKLISLAQDPDPVKAFEGVST
                     WAEIAVKAIEKKKKDLISPINDDEQNILKIIDDAFARITRANAAVTAQLNSIRKVQEV
                     QDDALKALNIKELRDLINNQLISASNKATSAIEQVAKAEKIIPDIDEKKQIIINKIKG
                     EK"
     gene            12616..13020
                     /locus_tag="PLT63_01110"
     CDS             12616..13020
                     /locus_tag="PLT63_01110"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOD97350.1"
                     /translation="MTTGKRIREIREKRGLTQEELAKKAGMSKGFLSDVENDKTNIGS
                     QGILKLANELGASVDYLLAGRVAKNVETKKIVIPQELSQAAEDLKLSYSTTLDLLNTF
                     NSAVARRGKTFHKELTVDGWKKFYEAIKEAIE"
     gene            13021..14058
                     /locus_tag="PLT63_01115"
     CDS             13021..14058
                     /locus_tag="PLT63_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97351.1"
                     /translation="MATRLNRSTRIWRLAKDLGIRTVTDPVSDILNLCEKRIKKMLKD
                     ATDCRSLSSFLDWIAGNLGTSFEEIRSDDDFLRIKKKYLALGEKIFATLEQEFSDEVL
                     GITYRLSHRESWEPQFVSVIDCRGGNSIRAYYTKWHEVAHLLILTDQSRLCFRRTEHL
                     PDKDPEEVLIDRIAGKFGFYPPFIKPHIKGEISFEQIETLRQALCPEASNLASLIGFV
                     KAWPEPGLLVLCKEGLKKSESRRLSIQRSFDFSTLPVPVLRAIKVTPNEAAKEQGILI
                     HENMRIPERSVIYAVHNSGEAESAEAMEDLSWWESSSGAILPKQKISVKAKDFWGDTY
                     ALIIPKFRSRS"
     gene            14268..15029
                     /locus_tag="PLT63_01120"
     CDS             14268..15029
                     /locus_tag="PLT63_01120"
                     /inference="COORDINATES: protein motif:HMM:NF013729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1883 domain-containing protein"
                     /protein_id="HOD97352.1"
                     /translation="MEHQYYDLHQLRGGEVVEVQLTNAANVRLMDSSNYSNYKRNGQH
                     RYIGGYVKQSPYRITVPNSGHWYVAIDLGGHAGRIRSSVRVLPGILPTASNAPLSSMP
                     SLRLDRSEIPEEDNRVREYDVFVSHASEDKELVARPLAEKLRKLGLRVWFDEFELKIG
                     DSLRRKIDKGLANSKFGVVILSKSFINKGWANYELDGIITKSVSGQQVVLPIWHDITK
                     QEVIDYSPSLADKLARNTAISTIEEIAEEINEVVR"
     gene            15157..16113
                     /locus_tag="PLT63_01125"
     CDS             15157..16113
                     /locus_tag="PLT63_01125"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HOD97353.1"
                     /translation="MHRQEPHVFISYLREDREDIDRICDALKRKDIQVWIDREKILPG
                     ERWQIAIRRAIEEGAYFLACFSDAYAKRETSYMNVELAIAVEQLALRPTDRAWFIPIV
                     LKGGRVPDRPIGGGETLRDVQWVDLAEDWEEGLRRILTVLRPEAAHRKGAVEPEYAVI
                     LMTDIVGSSRISQGLDQDGFHRTIVDISLSLMESAAEHGGRQVASSGDGMVATFDSGT
                     MALRCALAINRFVGSYKVNEEPLKIRIGLAEGKTFDTDKGFEQDAHHVAARICSAAGP
                     GEIFCTESLGRLAASQNWEYRDRGWVSLKGFAEPTHIYEIIS"
     gene            16471..17226
                     /locus_tag="PLT63_01130"
     CDS             16471..17226
                     /locus_tag="PLT63_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97354.1"
                     /translation="MQKQDSWPEINSKILDVIMENNYLPSPAEQADNLILWIGDNVEV
                     PGVEIGVDPDLHKAIIGAVNNHNFHFIVSSLVEQGLVLSKSEMVKRPVTLSFRGWQRY
                     ELIKKGALDSRKAFMAMPYGNDLIDKVYRDYFKKAVEQTGFNLFRIDEKPKAGSIDDR
                     LRVEIRTSKFLIAELTEGNHGAYWEAGFAEGLGKPVIYTCESTYFKNKGTHFDTNHLH
                     TVLWEREGLEDAAERLKITIRATLPEDAKLKDD"
     gene            17451..17753
                     /locus_tag="PLT63_01135"
     CDS             17451..17753
                     /locus_tag="PLT63_01135"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HOD97355.1"
                     /translation="MSEILDNANEMARDLFKVGAMDEITMRKIDALCLPTKRTFQPED
                     IRRIRSANHVSQAVFAAVLGIGKTTVQQWEQGQKKPSGPAQRLLDLIDRKGLVVLG"
     gene            18004..19125
                     /locus_tag="PLT63_01140"
     CDS             18004..19125
                     /locus_tag="PLT63_01140"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD97356.1"
                     /translation="MIKREIQAVIEQTLFKGKAIIIYGARQVGKTTLIKAIQQNATVP
                     SLYLNCDEPDIRRALSDKTSTELKMIIGNNKLVLIDEAQRVSTIGLAIKLLTDTAPDL
                     QVIATGSSAFELSSRIEEPLTGRKREFRLYPFSLMELGQMYSPIEIRRIVERCMVYGL
                     YPEIINAPEAAGTALREIAKSYLYKDILTFQQVRNAEALERLVQSVALQTGNEVSYNE
                     LAQQVGVDKKTIESYLRILEQSFIIFRLGSFSRNLRNELKKSRKIYFVDTGVRNAVIN
                     NLNPPDLRSDTGGLWENFVIAERMKRNHNQQLFPNTYFWRSHQRQEIDYLEEQNGELR
                     GYKIKWRETRLKIPAGFSAAYPDCPVSLVNRENVAEFVR"
     gene            19160..21337
                     /locus_tag="PLT63_01145"
     CDS             19160..21337
                     /locus_tag="PLT63_01145"
                     /inference="COORDINATES: protein motif:HMM:TIGR01056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HOD97357.1"
                     /translation="MIRVIVSEKPSMGRAIAAALGIQGTGRSFIQGKDVIVTWCVGHL
                     VQAIEPEGYNPDLKTWRMDTLPIFPDQFRYAPIEATRDQYAVVASLMNRDDVADVVNA
                     TDAGREGELIFDLVYRLSGCAKPVQRFWTSSLTDNAIREAYASMKPGKAYYGLRDAAR
                     SRQESDWLVGINCTRAQTLVMRRSGGEGVYSIGRVQTPTLAILVNRELEITDFVPKEF
                     WTVWAVFQAQEGIYRGKWFHKVDGKDQDRFDREEDAKDIVNKLSGLPGKVVSVISKTE
                     KKKPELLYDLTTLQKEANRRFGFTAEHTLEVAQELYEGKLISYPRTNSRYLTEADAQK
                     ATGWIKAVAQGQLMDLQPFIGELRKRWPVKLDKRFVNDKEVEDHAALTPTENPAGNLH
                     GDLLKVYELIARRFLAAYFPDRIEGKTTIITKIAKETFKTTGTLVKELGWSAVDPPHG
                     RPKKEKARTKGPADEIEEDEDTGLLPSVVKEETVDTKDIMTRAGKTSPPKRLTEGDLL
                     GAMQSAGRELDDEELKGAMKDCGLGTPATRANIIETLLKRGFVERIRSVLQPTAKGIE
                     LIQSIQAETLKSPQMTGEWEARLERIRRGEAKRDEFMGGIRTFVTELVGQIEQQVPQQ
                     QRTFGAVVGTCPKCGSNLLLRSWEDRYYVKCAASANPKCHVSFDANAEGKALKFCMFC
                     RGPVRTTRNGGKVCAQCNRWQSSRRKGYAASGRSSAGPSGSLF"
     gene            complement(22009..22938)
                     /locus_tag="PLT63_01150"
     CDS             complement(22009..22938)
                     /locus_tag="PLT63_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97358.1"
                     /translation="MKKRLSALPVIVSFLLFSSPLSVCLSFTDATSAGITAKKQPSTA
                     PAHDPAMPALLGQVRLDLSTVLQALFYRLQGREMHAPEVVAFDPSVSDLRRDVETEID
                     LSGFVLQRISIRRDEEVGNDPLHRRIEAVYKIADSIGRQMFTVIAADYIISEKAVLIR
                     QAVAIPYYPDGADIRFLILPANKLPALKLLKGLSFEELLSLVAENALSRKELERLKPG
                     QPGRFKLAAFNMIKAEPGKSLNIYTSKDDVSGSIRIKDSLSLNKNGWTAALVDGEFMF
                     NTAPGFWFYVGMSDAKESGKTRLLERFYSRIKQ"
     gene            complement(22952..24601)
                     /locus_tag="PLT63_01155"
     CDS             complement(22952..24601)
                     /locus_tag="PLT63_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97359.1"
                     /translation="MRLFYGMIILLVAVLTGIAPECVFSQTVGAAIEQQLKNAGVDAK
                     DVGTIIQGSEKKAGMSFNVPLYDRVDFATFPDARLNVTKLAAPAALVLTPQFSNYIFR
                     GKSDSFQGSFRTYEFHLGEGAHKACLMIFPQVFEHIRGVSSHQGLGAEEVAIVPRLDE
                     FRFKWGGTWTPVMSVTMKMSVGLFAQGRQIFEKTYQVEDIKEGTSTIFPNESQEYKAI
                     SKAMITVLTQAAREIAVLPVLLAYKVPSPPPAEESITQIPAKKIDMDAIVFLAGTWEK
                     YRENINRLTFDERVVARRILRLKDAWSSKDLDRQQDEINFLIQIAKKLEPVSRNDLLN
                     EIKLHKKWTDQGIVERNAAEDKIYNFAGDYTETVLDYAGLGIDLAKVGVLATGCLYSL
                     GTICPALALTMSEFDLASTLAEGVGAATAEAFWKSGDPSKAVYEAAKASLVDFTAGKI
                     GGAAVDTVTGKLALRAAQKNAAGAASEPLKNKIFVNTMELSRSPTEGLKALAKEANKR
                     IATAGENMITKGMTEEKTLKPPPRTGQAQPQLPGFESLNRL"
     gene            complement(24819..25628)
                     /locus_tag="PLT63_01160"
     CDS             complement(24819..25628)
                     /locus_tag="PLT63_01160"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOD97360.1"
                     /translation="MTEYGVRFERKGKVALITFDRPERRNAFNELMWSDLEQKVRKLR
                     EHLPSVIIVTGSGKAFCAGFDINPDNPQVSGLIESVQKQERPPVEILIKRLQSAVNDL
                     VMLPVPVIAAINGDAYGGGAELASRCDLRVMDPRAVICFAEVKLGLMPDWGGGPGLTR
                     LIGASRAADMILTGRKVGANEAMRIGLINRISAQGMALDEAMEMAEMIAKNGPRAVRH
                     ALTVIRQAQELTMKQALELECQEAITLITSGECIQGITAFLGKQPPEFPDI"
     gene            complement(26161..26436)
                     /locus_tag="PLT63_01165"
     CDS             complement(26161..26436)
                     /locus_tag="PLT63_01165"
                     /inference="COORDINATES: protein motif:HMM:NF015947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOD97361.1"
                     /translation="MRTLTVSAKGQIALPKDLRTILNLQKGDSLSVDLKDGKIILEPV
                     IGVPRSQSWFWTSEVQQKIKSSEEEFKNGKFKRFEGVDDLLKVLKDA"
     gene            complement(26776..27099)
                     /locus_tag="PLT63_01170"
     CDS             complement(26776..27099)
                     /locus_tag="PLT63_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97362.1"
                     /translation="MEGAQETEAMQETQETEETILYAKTKKGKRRHIVRTRDGNGQAE
                     MYCGKLYNEERLYQASDAVRVCRPCQREFDREVNAEAEHAEEPEGEQVETEQAETEQE
                     QEQQP"
     gene            complement(27120..27896)
                     /locus_tag="PLT63_01175"
     CDS             complement(27120..27896)
                     /locus_tag="PLT63_01175"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HOD97363.1"
                     /translation="MLSMFAWTQTCEARAKHVFEIGPELSYIQYKEPDIMDEKGPSIG
                     VSGSYTYHNKIMARIAGRFSYSRMDYDSQDTGSISGINDYGYEVRGLLGYDFKPSKTF
                     TISPFVGIGYRYLCDDVSGKTSTTGHKGYKRESNYIYSPIGIEASKAINKKWSYGASA
                     EYDLFWTGKQTSHLGDVVRGIDKLDNDQNSGYGWRASLYVAMATRRATYALEPFVRYW
                     NIDKSDAKGITYRRYQTGWVGWEPENNSTEYGLKIKVIFD"
     gene            complement(28159..28626)
                     /locus_tag="PLT63_01180"
     CDS             complement(28159..28626)
                     /locus_tag="PLT63_01180"
                     /inference="COORDINATES: protein motif:HMM:NF019499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HOD97364.1"
                     /translation="MRTHLNTTRDIANIAVPLLGAGIMVYYGSCETTCATLSGSLFGI
                     DLKIVGVSFMVVLFLFNLIALPELRRYIVHLRASMLAAALGGEVLLVRFQIVHDTFCP
                     YCLAFMACLVALFAFNTGRFTNWRIAGGCFVAGVAAFFLWFEGAVGPMLFLLD"
     gene            complement(28647..29270)
                     /locus_tag="PLT63_01185"
     CDS             complement(28647..29270)
                     /locus_tag="PLT63_01185"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOD97365.1"
                     /translation="MQQIYNVVIRHPASASCLVVTLVSLFLFATSAPVISASPRTAAN
                     QPAYPVYGNGPVEVCLYTDYFCPPCRAIKASVDPLVRKLVELNRIRVVFVDVPLSKQS
                     IEYAKEYLRCLHAYDNNLDTAITLKAHFNYAAENGRSIEAYLDTAGIVRTQERNSCVS
                     TIFSFYNIKIKEDRVRATPTCVIAGENGFKESYTGGKAIVAALQRLL"
     gene            29584..29871
                     /locus_tag="PLT63_01190"
     CDS             29584..29871
                     /locus_tag="PLT63_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97366.1"
                     /translation="MPEGKSLLLDEEGVRKNWAAHINANPLDRPRLEAIHGQVWDTEE
                     LSRDYDVHGFMAPLVIATCKATKKRGSLLFQHHPRFYFGFVKVATEDNDAQ"
     gene            29861..31492
                     /locus_tag="PLT63_01195"
     CDS             29861..31492
                     /locus_tag="PLT63_01195"
                     /inference="COORDINATES: protein motif:HMM:NF025085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HOD97367.1"
                     /translation="MLNNDTFYPTPVPLIDRMIAKIKGRPQTVLDPAAGMAAIIERMR
                     DYSTMDSDNRYCYRPYENCSYSAIEIDTNLQAILDKKPFVSLIDTDFLQYSGRDHFDL
                     IIANPPFDRGDLHLLKAIDIMYRGEIVYLLNAETIRNPYTHSRKELAQRLAERNAEIE
                     FVPDAFKDAERQTDVEVALIYIRVERTVEEDLFAGVEDEAQECDATAAKQDWEVATKH
                     RIRDMVADYNRTIDIGTKTILDYYKNYNKIGKYLGLNCEAKERLYERRDDLTSLVRGK
                     VNDFIKSVRLNYWRQALELKEVRSRMTTDKAKEFESQIQKRQKMDFTESNIRQFIVNL
                     INGYEGMLTDAVTKLFDKFTIEHCYSGGIVEENIHMFNGWKTNQAFKVNKKVIIPIGY
                     RLSDGEGPFTYGTYGHRWKANSWKISEKTRDIDIVMSYFSADTDYVPIADAVAAALDR
                     QESKNIDTTFFRITCYKKGTMHMVFKDDDVLRRFNIVACRAKGWLPMDYGQKRYEDCD
                     EETQAVVKSFEGIPQYNSNVKRPLFATKPLLAIAA"
     gene            31547..32155
                     /locus_tag="PLT63_01200"
     CDS             31547..32155
                     /locus_tag="PLT63_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97368.1"
                     /translation="MSQTIVIEDSKFSHSLAGHFANVCWVCKAAAKEPDVRASIKQRI
                     ACREGILCATDGYRLHLYRQDSDSLPDSAIVPEGVWAIKSKTARVITLELAEDEFAAD
                     FPNFWIVFQDRPKVLGSFSSLGASGKGDYPYVFGEFLYRLNTHAGKAFRADHVADAFM
                     PDRHIQVEAHAKGKMAVFGDDTLIAALMAIITTDNGGNRRWA"
     gene            32146..32484
                     /locus_tag="PLT63_01205"
     CDS             32146..32484
                     /locus_tag="PLT63_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97369.1"
                     /translation="MGMMGGQKSGWVDTAPEKNEAIARMLWPDAYAGAGTNKMQAPDF
                     TTPEGRLILLQLMMERDDWLMFSMLKSVAINHRGTIFWIDTGYLFLPDKLRDAALGWL
                     AGSNTGGRSV"
     gene            32486..32746
                     /locus_tag="PLT63_01210"
     CDS             32486..32746
                     /locus_tag="PLT63_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97370.1"
                     /translation="MIGRTVAIIAFCLSVAAFCSVLATYIRGALQAGKDGPAQQHVAS
                     PYIKKQMQYHGVNVLFEDWKGNLFFERQGQKIVLGQGREIDK"
     gene            32743..33186
                     /locus_tag="PLT63_01215"
     CDS             32743..33186
                     /locus_tag="PLT63_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97371.1"
                     /translation="MTKMHQVRKEFGEPFRDVVKGFAIMGYSRKATAEFVEITDSWFN
                     ELCERFDLRKHFRRQRKDFVSVCKPPGHPKGKTIRQPQRYSDEYLLAVLRGYSKRISQ
                     VRFNQLQGGRPCADTYLKRFGSWRKARELAAKCEDERERNGENYG"
     gene            33179..33511
                     /locus_tag="PLT63_01220"
     CDS             33179..33511
                     /locus_tag="PLT63_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97372.1"
                     /translation="MDSNKMLKTVLPEGCKFRNLAKLGEKPDWEIVPKRKPRRMSYAE
                     VLQVITKYAPEMQHCFENKFGRFPKGGEAAMIVNYLNDTADLYRAERENIVNDLVDAA
                     TEIIVRCG"
     gene            33856..34590
                     /locus_tag="PLT63_01225"
     CDS             33856..34590
                     /locus_tag="PLT63_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97373.1"
                     /translation="MARFKTMVLMAVTAAMMAGCGAAAPQYLDYLGHRIPTTKLSAPQ
                     DIAAKMAQDGVVSMEAFGALSAGKAAGAANAAALQVEASSVETIRLDPNDSALIVSVR
                     KDGVDPNRMPDAFRKSYFKALSQVNAVSAALSFSKPLEISHVLKGRHDYFTNHLGTEA
                     SRKRLKEKLAMCQWEGTAIPVKKEGNGAFYYADMQNYAITLSEFAFQIKYSDKEKALP
                     DTDPCSAGEADKLLRQLIALIAGGEK"
     gene            34587..35333
                     /locus_tag="PLT63_01230"
     CDS             34587..35333
                     /locus_tag="PLT63_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97374.1"
                     /translation="MKKIIPKLLLLVVMAAMMAGCGKNHVLVAGRSIPLYEMESVADI
                     DKKMAEDGAVSMKDFAAARAAEARQRADLAGVESAALLTVDAALYGMQFAPAVFGTMS
                     AASNAAMAYLSIGDLITAYGRYLERKNSAYAGLKFSKPLEIYRVRNGENVFFADLLGT
                     EDYRRNLRVKMATCGWLETIIPDKKKGNGAYYRAGTDNSSITANELLFQIKYGDNAAS
                     LPETNPCSIKEAEMLLVQRVALIMGSKAKK"
     gene            35345..36163
                     /locus_tag="PLT63_01235"
     CDS             35345..36163
                     /locus_tag="PLT63_01235"
                     /inference="COORDINATES: protein motif:HMM:NF019146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5131 family protein"
                     /protein_id="HOD97375.1"
                     /translation="MNVEKGRYWDKRWSLVDCCTRPDRLNIPLRTRKPTIFAVRNDLF
                     HEAVPASFIDDAYEVMAACPHHTFLVLTKRAQNIVPKLYDITVDCPIRELGGGDYLPN
                     VWHGLTICNQPELDAKMGDFLKVPGKKWMSIEPMLGEIDIKPYLSKPMYYCSVCGKEK
                     FRDYCKKCGTYDNTATNKLAGIDGVVLGGETGPGPRLMHPDWVRNVRNDCAAAGVPFF
                     FSQWGSYTGIRATGYDFHNEVLNPAGHNRTKSNGGRLLDGRTHDDLAWGKKEGI"
     gene            36160..37986
                     /locus_tag="PLT63_01240"
     CDS             36160..37986
                     /locus_tag="PLT63_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97376.1"
                     /translation="MNRYFFGNRGGKYLLIKAPDLETAIKVFAVVELFPEQYLPGDMV
                     CSRKYDSVLERYVIEPKPRATIPVYIEVEETFVTKSSVSGITRTETCKVQKKIHDLLL
                     ADCSEHHLPPSSILPEKTGDDETTGQITSVVINGQAIIVSGSKAALRNSHDELQRKKH
                     ELEVMQRQCRAAVEAIQKDLKKKQELLYAVCTYMGQGEEIVPLLTGQNAPEDAPLSVF
                     QEVLYMDEEMGAWDKDSLDGIDFSRIEEFDAWIARHYETFLYREKSVCVFQIRRYSKD
                     YGDPLENAVLNAENMKSYFLVRNGGNLYRIYGNVHIREPVFPTKDEYESIIAKYRDTN
                     SSWGEKNLNNRHKHYMYGLIYLQGLIERSSIFGNGLKGVSLPKGIFTQAQIELIRDSE
                     QSQWITDGKPSWNDFRKNNQATIGIGSRIVLMPVRFSPKDDLWRLGNTPAAGLPPWSE
                     TYLVEAEGDGWGYMAKGSFIIRYNSADTIHDEYEGFRPRKRRVSYRFFPDEVINFDRI
                     TVEECDYYIKNRLERKHYRDILPVLYYVRRCKERERALENNFVSFMAGRMSLDERLLP
                     ALREVIAWWKLKNKWKRGLMTDDQKAVRMIEKKMQAIAAEGI"
     gene            38138..38428
                     /locus_tag="PLT63_01245"
     CDS             38138..38428
                     /locus_tag="PLT63_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97377.1"
                     /translation="MPDIDHNDYMEFLCWVDRCLFPQDLMEELVAHCGATHEEAKEIA
                     LLFFSWKGTDSKEGKNLSWAYSNYCLEPGRYKEIINSYLKWQYGKWRKLRCR"
     gene            38419..38946
                     /locus_tag="PLT63_01250"
     CDS             38419..38946
                     /locus_tag="PLT63_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97378.1"
                     /translation="MPLGVVEKTGYNPPGVPTNGRKPHLGDIILNKFYHLIREGREDL
                     EQFELIDRLEKPVMYFVFSGTKRQILEVNKLLQQHPYAFAIKTNLGYDLSKLPEKYAH
                     TAQVYISRFPHLIKFISKYESTIPSDLWGILFGYKLNEVHLYTYDWVEWAKIHLPDRV
                     EGKRDTATRDFRVEI"
     gene            39115..39191
                     /locus_tag="PLT63_01255"
     tRNA            39115..39191
                     /locus_tag="PLT63_01255"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39149..39151,aa:Pro,seq:tgg)
     gene            39197..39493
                     /locus_tag="PLT63_01260"
     CDS             39197..39493
                     /locus_tag="PLT63_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97379.1"
                     /translation="MRDTPGLIERFEFIAADGGRVERAVPPHLKETLAWEVDTTCPQR
                     CKLATCCHPLVQDNKDKYVYHKLCTLSVIKAGLISPPEINGWIESEEEGNEEHD"
     gene            39486..40364
                     /locus_tag="PLT63_01265"
     CDS             39486..40364
                     /locus_tag="PLT63_01265"
                     /inference="COORDINATES: protein motif:HMM:NF039495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97380.1"
                     /translation="MTEGRQMIASIPVLGVIKHPRDGKHFIVPPEVAAVYGENPCRLD
                     FMLPGDDVEAVFQEAYRRFVGGGGLRCIGSNGVGYDRDFKTGNRVQKACPCEFLDNNQ
                     CALTGAFHILIPRVSLGGQFVIYTKSKRSSAMIRMTLKNLQGMYGKVTGLSMSLTRVP
                     VKIKYDKGKNKEYWMLNIVPPTMDVVAQAMPADPHSTEAWRSRQEAGEENMEQAQAEG
                     EAQQIVPMTDEQRRRLVTLMEAKGIGTSADKAAFFAHVLGGREKSEKFARDFIANFEE
                     KHRAWISRIMEEAVAS"
     gene            40367..41026
                     /locus_tag="PLT63_01270"
     CDS             40367..41026
                     /locus_tag="PLT63_01270"
                     /inference="COORDINATES: protein motif:HMM:NF012304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase-like protein"
                     /protein_id="HOD97381.1"
                     /translation="MLSFNNSIGFDMHGLSAKAWFDGACAGNPGPMGIGGLIILEGGE
                     KHTISEQRGFGTNNVAEYTALIEVLKLAIELGVTDLQVKGDSQLVVNQVNGDWAVKNR
                     GLVPYCDAAEVLMARIPRFKLQWVRREQNQEADRLSSSALKGGGKNGEAQGSRQRKNL
                     SVAVRKVGDHIYVAEGSHGNQYAVDLKARTCSCPDWQRRQSPCKHMMAAERAHEVGHD
                     S"
     gene            41016..41225
                     /locus_tag="PLT63_01275"
     CDS             41016..41225
                     /locus_tag="PLT63_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97382.1"
                     /translation="MTVEDIHQYGFDLGEKDGQVAARWIRKGIEVDHGDLLSATDSYA
                     VGYRKGLNWSIRPSITVIRRKKNNA"
     gene            41218..41802
                     /locus_tag="PLT63_01280"
     CDS             41218..41802
                     /locus_tag="PLT63_01280"
                     /inference="COORDINATES: protein motif:HMM:NF016246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HOD97383.1"
                     /translation="MPEIWTIGHSNHSVEHFLHLLSMHGITAVADVRSTPYSIAASQF
                     NREAIKEVLHKERIYYVHLGEELGPRSPDPACYENGKVVYGLLAATAVFAQGIERLRK
                     GMERYRVAMMSAEKDPIACHRMVLICRHLSMHADDGLAIRHILEDGAIEDNAASERRL
                     MQDLKIAEFDLFNSREEQIAKAYALQSQRIAYAL"
     gene            41827..42072
                     /locus_tag="PLT63_01285"
     CDS             41827..42072
                     /locus_tag="PLT63_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97384.1"
                     /translation="MKKSVLLFLEKVFAANLDGRLLQSKSKVAKKLEEAGYIVFTEKT
                     LGRDRFGSIVVDGYTLTVMGNMAYCTSDRCKEPVEEQ"
     gene            42179..42436
                     /locus_tag="PLT63_01290"
     CDS             42179..42436
                     /locus_tag="PLT63_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97385.1"
                     /translation="MPMNNNDVEALKSKAKVAANTLYRICHTGRRSAETMELLDITSE
                     VLALLNDLVDSLNADASSATNDDAYVRAKIFLNAWSGRVNG"
     gene            42429..43103
                     /locus_tag="PLT63_01295"
     CDS             42429..43103
                     /locus_tag="PLT63_01295"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HOD97386.1"
                     /translation="MANNSKEHKKNPLHTPRVIAYVRVSTDEQDVNKQRHEILEYAHQ
                     NKMTIDEFIMITVSSRKAAKDRRIEELMEKLAPEDTLIISELSRLGRSIAEVIGLVNG
                     LVERNVRLIAIKQNIDLAGKHDTTSKVMVTVFSLLAELERDLVSQRTKAALAALKAQG
                     KKLGRPVGTKGKSKLDGKEPEIIAFLDKRVPVAAIARILGCSRPGLLNFMENKKIYKA
                     KRSEKR"
     gene            43513..43589
                     /locus_tag="PLT63_01300"
     tRNA            43513..43589
                     /locus_tag="PLT63_01300"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43547..43549,aa:Asp,seq:gtc)
     gene            43644..44516
                     /locus_tag="PLT63_01305"
     CDS             43644..44516
                     /locus_tag="PLT63_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOD97387.1"
                     /translation="MKYEGIIIRPPSEAYSLLLQVTVGCSHNRCTFCPTYKGVKFRIK
                     SPEEISEDIRAARRYRQVEKVFLCDGDALIVPHARLLDMLHQLKESLPALKRVGLYGN
                     AKSILRKSREELGELRETGLGIVYLGVESGHRDVLRDIKKGVSYDQLVEAGRRVKDAG
                     ISLSVTVILGLGGRDRSVDHARETARILTDIDPDYVGALTLMLVPGTPLHDEYDTGKF
                     ILPDTFDFLNELGIMIANSTFTNCFFTSNHASNYLPIRARLPQEKEKTVALIQKVIGA
                     ANPGLLRPEYARAL"
     gene            44538..45374
                     /locus_tag="PLT63_01310"
     CDS             44538..45374
                     /locus_tag="PLT63_01310"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOD97388.1"
                     /translation="MKLISLIKKIFQERDASRLEEEIQAIIDAGEEDGLIDPQSGEMI
                     QSILEFKDTVVREVMVPRTEIVAVSAGATIEEIVRIISIYGHTRMPVYRGDIDHIIGV
                     VNVKDLLKFWSREVTEKDFLSILRKPYYIPETKNISQLLHELKQKKYHLAIIIDEYGG
                     TAGLVTLEDLMEEIIGEIHDEHDTQEPGIVEETDGYTLVDGRVEIEELEEYFGVTFDE
                     GKYETLGGLILNTVKRIPAAGETIIVDNFEMTIVAADERRIKKVRIRKIRKEDEQNSP
                     EA"
     gene            45349..46935
                     /gene="lnt"
                     /locus_tag="PLT63_01315"
     CDS             45349..46935
                     /gene="lnt"
                     /locus_tag="PLT63_01315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOD97389.1"
                     /translation="MSKILRKPEGGRSSRLDSFDLLLALGSGVLLIFSFPKFGTGLVA
                     WISLLPLLYSLRGKNASHGFLLGLATGITSQIGVMYWITYVVVNYGYLPYPVGIAAML
                     LLSGYLGLFTGVFAAAAAYLLGWRLPFCLTAPVLWTVLEFGKSHLLTGFPWENLGYSQ
                     YLNLPILQIADVAGVYGVSFLIVFVNALIFEVVFINRGKEERKERVVKIIAVLGIMVL
                     LFGYGLMRLKEIDRAASAPSMTVRITQGNVDQSIKWNPAYQLKTIAAYAALSRSEAVT
                     EPALIVWPETAYPAFFQDVNDLHRKVVGLVKDKAAWFLIGSPAYTTQSGETVLFNSAF
                     LLTPEGAIAGRYDKVHLVPYGEYVPLRKFFPFVNKLVAGIGDFGVGPGYYPLTMNSRR
                     LGVLICYEGIFPAAARDYKRRGADFLVNITNDAWFGSTSAPYQHLSMTVFRAVENRLD
                     VVRAANTGISARIDAAGRIDGRTELFQTAFQDIKVQFYNKGTFYAVWGDLFVYLCIIA
                     AVLFFMIIPIRRKRDAGRDK"
     gene            46916..48008
                     /gene="prfB"
                     /locus_tag="PLT63_01320"
     CDS             join(46916..46984,46986..48008)
                     /gene="prfB"
                     /locus_tag="PLT63_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139198277.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOD97390.1"
                     /translation="MLEGINEKMTDLKRRISHLGECLDVATKENEIRELEKTAAREDF
                     WNDQEKASAVLRQQTKLKEAVTHWRNQQRQYEDLAMLYDMAAEESDEAALMDVGRDIE
                     ALEKSVREEELRLMLGAPEDRLHAIMSIHAGAGGTEAQDWAEMLLRMYLRWAERKGYK
                     ATIIDYLPGDEAGVKSVSLAIEGEYAYGYAKAEVGIHRLVRISPFDAGARRHTSFVSV
                     FVYPEVDDSIVIEIDEKDLRIDTYRSSGAGGQHVNKTDSAVRITHLPTGIVVQCQNER
                     SQHKNKAMAMKYLKSRLFEIKLQEQNEKLDEVEKTKKGIAWGSQIRSYVLHPYKMIKD
                     HRTNLEVGNVTRILDGDIDDFIEAYLLAN"
     gene            48249..50078
                     /locus_tag="PLT63_01325"
     CDS             48249..50078
                     /locus_tag="PLT63_01325"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOD97391.1"
                     /translation="MRTAISSPPAQTGSKGGNDPVPVAARSVLPLSGEKGNVPSEVKS
                     PENRAKGKEDQQEQDIMEAALRLLSESQSYWVKGDVENAIEMLDQAYALILETNGDPE
                     IGRQKDDLRLLISKRILAIYSAMQSAAKGKRGEIPLIMNADVEKEIRSFQTVERDFFI
                     SSYQRSSLYRPYMVKELKRLGLPEELSWLPLVESGYKIKALSTARALGLWQFIPSTGY
                     KYGLERDQWVDERMDPEKSTRAAIAYLSDLHGMFGDWLTVLAAYNCGEGRVMRVISRQ
                     HINYLDRFWDLYNQLPYETARYVPRFLATIHIVRNPAKYGMDLGVPMEAQGKGRGYET
                     VKTNKSMRLKDIAQHLNVPEEALCQLNPELRHMITPEKEYLLKVPPETVEKFASVADS
                     IPHWEQPPPTPRTRPVVIVHRVKRGETLASIAKRYRTTTKAIIRCNNLRAKRGITVGQ
                     HLSIPIREVKATKKSTYHVAAGTGKKAGGGEKVIRYQVKKGDTLSSVAARNNTTIGSI
                     KKANGLKSNVLRTGQVIRIVKTDAAGGPDTGKKGKRGAGTTKTGKSRSAAQVKEKTYT
                     VRKGDSLFRIARSHDIEIDRLCELNNMSLKEAQLRAGQVLVVE"
     gene            complement(50122..51483)
                     /locus_tag="PLT63_01330"
     CDS             complement(50122..51483)
                     /locus_tag="PLT63_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HOD97392.1"
                     /translation="MNAEVFREYDVRGLVGRDLSEDFVYHLGLAIGAYGRRHNVGIMT
                     LGRDCRLSSEGFSQIIARGLSDAGIAVIDLGMCATPMLYYSIRHYETHGGVMVTGSHN
                     PPDFNGFKICIGPDTIYGDEIQELRRIMEAESYGRRGGGTVTTRDITSDYQDYLVGNV
                     KIQPGLKIVVDAGNGTGGLFSLPIFRRLGCIITEIYCEPDGRFPNHHPDPTVPDNLRE
                     LIATVKEKGADVGIAFDGDADRIGVITDRGDVLWGDELLLLFSRFLLREKPGAAIIGE
                     VKCSQKLYDDIAAHGGRPIMWKAGHSLIKGKMKTEHALLAGEMSGHLFFADRYFGYDD
                     AIYAAIRLLEILSQSPGKKIGDLIADVPKTFSTPEIRIDCPDEVKFKAVEAIKEHFRK
                     DYEIIDIDGVRIPFADGWGLLRPSNTQPVLVLRFEADSTARLAEIRALVEGVLNQTLA
                     KMI"
     gene            complement(51554..52210)
                     /locus_tag="PLT63_01335"
     CDS             complement(51554..52210)
                     /locus_tag="PLT63_01335"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOD97393.1"
                     /translation="MKTIVFDFDGTVAGLTIDFAEMRRRVMDHLQSFGISPDGLRKLY
                     ILEMIAAGKKLIASSMPGRETNYERGALTLVRDMEVAAAQTGVLFYGIREMLSLLRAQ
                     GRHTGVVTRNCREAVEAVFPDIDVFVDVVLTRNDTPHVKPHPGHLHQALKTLAAPAAT
                     AAMVGDHKMDMHLARDVGVLAIGVLTGHDTARELKEAGAEVILASAADLADFLSSGSV
                     "
     gene            complement(52241..53113)
                     /locus_tag="PLT63_01340"
     CDS             complement(52241..53113)
                     /locus_tag="PLT63_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97394.1"
                     /translation="MIRKHDISAADGNLPLLVRSLLFRNQNDFLPKWNEIKEENSCRH
                     GRQLSAGVLLLLHRRPHGHPEHQKGDNPWVLQLIKRSSQTAQGGDISCPGGMLQHLID
                     WGFSLIFVRSGILPIPLLSVETGGMGTCSRERRIVSMFFANALRESWEELHLHPFRVS
                     LLGVLPTYSLHLFRRTIFPLVALTDPHWTPSPNREVEKFIEIPLESFFAPSSYGRYLI
                     KASDRVATADPGPWEFPCLIHGEDGREEILWGATFYIIMNFLKIVFNHQLPDLTDKRI
                     RSKVLHADYLTGKR"
     gene            complement(53110..54081)
                     /locus_tag="PLT63_01345"
     CDS             complement(53110..54081)
                     /locus_tag="PLT63_01345"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOD97395.1"
                     /translation="MNNIFAPGLFFRNPHLQTFLGRSKYRLKDTEELHRSSTPIILPC
                     GEGVRLLGLHARHPEGNSRGLVALIHGWEGSADSTYILATAGYLFRLGFDIFRLNLRD
                     HGDSHHLNEGLFHGALIRETFQALRNIARLPTFGRLYLIGFSLGGNFALRAALKHSRE
                     EELPPLSHVFAVSPALDPHKATVAIDAGWGVYRRYFLNKWKRSLNKKQALFPHLYDFR
                     PMMKTRSCMELTEAIMVYYREYRDYLDYFRQYTLDGASFIGLSVPTTIIISRDDPVIP
                     IEDFHALPTQEKLHVAVQPYGGHCGFIDMPGFSCWYERKIYDIIERS"
     gene            complement(54133..54209)
                     /locus_tag="PLT63_01350"
     tRNA            complement(54133..54209)
                     /locus_tag="PLT63_01350"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54173..54175),aa:Met,seq:cat)
     gene            complement(54268..56022)
                     /locus_tag="PLT63_01355"
     CDS             complement(54268..56022)
                     /locus_tag="PLT63_01355"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HOD97396.1"
                     /translation="MKQYNRFYFLTMAGICLVIILSAPLGAKDKKSVAVLPFAVNSAD
                     NIDYVQQGIQVMLASRIAASDKIEVVSKDKITDVVKTVKLKEMTLADINGLGKKMAVN
                     YVVAGTITKIGNSVSIDGRLVDIAADKPAVTVSTQSQGMDDVIVKINDFALRIEQHIL
                     GTAPAVAFGGPIPPAATTAATLPQITAPGASPVQKERDGQIIAGMRTGKKATFTGAIN
                     PDFISGAQPRDKRGFWMSQKYPTNYKGIDVGDVNGDGRNEMVVIDENSVYIYQKKGND
                     ALLLAKISGGRYNNYMGVDLIDLNGNGIKEIIVSNVLTKREANNVTNTISSFVLEWKD
                     GKFVTIADNLPWMFRVIDTKPAGIRLLGQKIGVSRPFETDINEMVWRNGKYQEGARLK
                     IPQGLNIYGLTLDNLGEGAEKIISFNNYDYLCVYHETDKPLYAVQSMLGSKEFIYKSE
                     EVFGGSNMYIEHYGEDMPGNDYTFYNTYLNARIIPYDTNKDGKRELIVVKNLTTSRIL
                     KNVKIFTASEFYNLEWDSMGLVENWHTRKMTGYVADYQIKDIDNDGKDEIVMVLVISA
                     GSLVGRESVIAAYNIHAQ"
     gene            complement(56308..57087)
                     /locus_tag="PLT63_01360"
     CDS             complement(56308..57087)
                     /locus_tag="PLT63_01360"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HOD97397.1"
                     /translation="MDILFLLKKIVSNFLMPLSVIIAFITTGLLMLGRKKKRQKTGIF
                     LIVLATILLAAFSYGFIANALVAPLENRYPPLLNPENHPQARDIRYVVVLGGGSFPEG
                     ELPLSSRLTPESLARLLEGMRIYHRLPGTKLLLSGGAVFQDVQPEAATLANVALMMGA
                     REEDLIREDQSLDTSDQARLIAAVIGKSPFILVTSAIHMPRSMALFRKQGLAPIPAPT
                     NYLAVPQTHFRPGSFFPGTVPLQKAEAAIHEYLGLIVTYMK"
     gene            complement(57087..58982)
                     /locus_tag="PLT63_01365"
     CDS             complement(57087..58982)
                     /locus_tag="PLT63_01365"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOD97398.1"
                     /translation="MNKEIHSKIVTPSEVLALLRPGMSIFLGTGAAEPRTLSKALLAA
                     SGNNINDLELIQLASIGEAIAFQPQAGRSKFRLKTFYSGWLAEAAITSGTVDLIPSRI
                     SRIPALIASGAIRVDVAFIQISPFDDADFASLGVAVDAARYAMEKASLVVGEINPAVP
                     RTMGNTFVHADEFNYLVRASETPIYLPRPAVDPVMERVADQVATVIPDGACLSFYPGT
                     LFESLGRKLTKKRDLGIHTFFFTDALMDLVNCGAVTNRYKGYFRNKSLTSYALGTPEL
                     MKWLHDNPRVEFQGIDIVSDHFRISLNDGMLAILPARKIDLTGIVALHTGQGNVGGGP
                     GQAEEFFAGSLHSRGGRAVFALPSRNLCGESNILVSAVRYPNLFSNREVLDLVATEYG
                     IASLSGRTLRERAQALIDIAHPSDRAELIRQAKAHHILFPDQIYLSESGAMYPFDIVS
                     DYPLKDGRKIHFRAIKPSDEEEMRRLFYQFSDRTIYYRYFSEIKVMPHEKMQEYVNID
                     YRRIMSIVGVVEEGGMPRIVAEGRYVRQEECPYGDTAFVVEESYQGNGIATALLQMIM
                     TVARKKGLRGVTADVLADNKAIIKVFEKGGLPMQAVMEHGIYHLTIPFPDLTDPDDYR
                     HTGQEFN"
     gene            59132..60469
                     /gene="purB"
                     /locus_tag="PLT63_01370"
     CDS             59132..60469
                     /gene="purB"
                     /locus_tag="PLT63_01370"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOD97399.1"
                     /translation="MIPRYSREKMAMIWSPEHRYQKWLSIEILACEAMSKLGLVPESS
                     LTIIKEKAGFDVKRIDEIEMTTKHDVIAFLTSVTEKVGPDGRFLHMGLTSSDILDTSL
                     ACLLQEAADILLADLDELLKVLKRRAHDHKDTIMIGRSHGIHAEPITFGLKLANWYGE
                     MQRNRERLKRARAAVSVGKIAGAVGTFSFVDPFVEEYVCVRLGLQPAPVSSQIVQRDR
                     HAEYFTVLAIIASSLDKFAQEIRLLQRTEVREVEEYFSPGQKGSSAMPHKRNPVLSEN
                     LSGLARLMRSYALAALENVPLWHERDISHSSVERVIAPDATILLDFMLGRFTRMVKKL
                     VIYPERMQANIQMTHGVIYSQMVLLELIAKGMTREDAYALVQRQAMRAWEEGKDFLTL
                     LKKDKKVREFLDDVQLAELFRTENFLGQVDLIFRRVFETEAAGGGFMSSRTET"
     gene            60561..61190
                     /locus_tag="PLT63_01375"
     CDS             60561..61190
                     /locus_tag="PLT63_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97400.1"
                     /translation="MKKVMPLWISMLILTFTGCGYLQGYLQMIEEQGVSEEYQRILRE
                     WTREMTAHSQFETKFYISATYRSLAFNEAFLREQARVRQWSEARVKEREEAWMQTDSA
                     YREFLVYAYTDNMEANDLDRQGSLWSVFLREGTGKRYEPLEIRRVERITPEIEAFFPF
                     VNKYYGRCYIVKFPAGETNIDQHLVFSSVLGKVDLVWQAGGHTPIAPSR"
     gene            complement(61202..62809)
                     /gene="nadB"
                     /locus_tag="PLT63_01380"
     CDS             complement(61202..62809)
                     /gene="nadB"
                     /locus_tag="PLT63_01380"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HOD97401.1"
                     /translation="MEIQTDFLVLGSGIAGLSFAIKAAELGTVAIVTKKETFESSTNY
                     AQGGIAAVQDEEDSFASHIQDTLACGADLCQEDVVRFVVEEGPARIEELVHWGVQFTR
                     REDSGGCRYDLGREGGHSRRRVLHAKDLTGREIERALTEHVSRKKSVHIYENFIAIDL
                     IMESFLTGRKDPAHNRCLGAYVLDFHNNRIHTFKSKYIILATGGCGKVYLVTTNPDIA
                     TGDGIAMAYRAGARIANMEFIQFHPTCLYHPDAKSFLISEAVRGEGAILRLKNGEPFM
                     EKYHPMNSLAPRDVVAKAIDTELKKSGDDYVLLDITHRDRDFIRRRFPNIYEQCLRFG
                     IDMAVDPIPVVPAAHYLCGGVQVNHHGETSIDRLYAFGEVSCTGLHGANRLASNSLLE
                     AVVFAHRAFVRLQSAFPRVKDEDIAIPPWDPREATESDESIVVSHNWDEIRRCMWHYV
                     GIVRSDKRLERAHRRVELISWEIDDYYRNFVVTRDLLELRNIATVAAIIIRCARMRKE
                     SRGLHYNIDYPDRDDVQWLRDTVIVKS"
     gene            62923..63930
                     /gene="tsaD"
                     /locus_tag="PLT63_01385"
     CDS             62923..63930
                     /gene="tsaD"
                     /locus_tag="PLT63_01385"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOD97402.1"
                     /translation="MLVLGIESSCDETAAAVVRDGKRVLSNVIASQVRIHGKYGGVVP
                     EIASRKHTEAIVSVILQALCEAGAELNDIEGIAVTRGPGLIGSLLVGLSTAKALAFAR
                     DLPLVGVNHLSGHIAAAMLMDPPPALPFVALVVSGGHTNIYLVRDFDDIQTLGQTRDD
                     AAGEAFDKAAKLLDIGYPGGVVIDQLAKQGDPGAVLFPRAMRDSLDFSFSGVKTSLLN
                     LVKKQGRPFSAAEIPDVVAGFQEAIVDVLVRKTLKAANQEAIRRVVVCGGVAANSHLR
                     SRFEHDAGDQEIEVFIPPPVFCTDNAAMIAVVGERLLSTGRRDGPDLNAVSRWPLELR
                     L"
     gene            63927..64379
                     /locus_tag="PLT63_01390"
     CDS             63927..64379
                     /locus_tag="PLT63_01390"
                     /inference="COORDINATES: protein motif:HMM:NF021363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HOD97403.1"
                     /translation="MNPIKRRLRDFHERFINLRGEPGHIAMGMAIGVFIGVTPTIPFH
                     TVLIIAVGVLFKQNIMAGYLGSWLISNPLTIPLFYFYQYELGRILLGIQTGAWVMPEL
                     TVAGIMNIGWHILIPLLVGGVVSAPFFAIPAYFFARRLAVAGRHREAA"
     gene            64376..65365
                     /gene="rsmA"
                     /locus_tag="PLT63_01395"
     CDS             64376..65365
                     /gene="rsmA"
                     /locus_tag="PLT63_01395"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOD97404.1"
                     /translation="MIPAGKILKKHAVRPRRRFSQSFLKDDNVARKIVGAADLRGQDV
                     VLEIGSGHGILTRLMAPRVKNLLAVEIDPQLVAVLEEETGSRDNVLILEADILKLDLA
                     TLLAGHGGERKKIVGNIPYGISTEILFRLLTHRRLIASAVLMVQKELAERLTAAPGTK
                     AYGIPTVLLTMYARIGHLFDVPPPCFHPRPRVVSSVISLTFRERPLVELSDENHFFRL
                     VKAAFAQRRKTIANNLRAAPELGCKQEDLEEMLEKCGLNGRIRAEMLTVETLGRLSNE
                     LTTARRGAPSSGEEGKGEGRQSEEGADKESALEGTGNGGHGIVTDMLLEFPDR"
     gene            complement(65471..65968)
                     /locus_tag="PLT63_01400"
                     /pseudo
     CDS             complement(65471..65968)
                     /locus_tag="PLT63_01400"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT        16553 a        16525 c        17612 g        15331 t
ORIGIN      
        1 ctgacaacgg tgttttgctt tatcgtcata aaactttctc ctttgagcta cattgcagcc
       61 caaaggagaa gataagtcaa tactattttg ggcctcgcac tacaagcgct caccggcaac
      121 caaggagaag atctgtggcc cggactactg aaagatgcag ccttttaata tacagcatcg
      181 gggtacgatg cagggcatcc gctgcattga ctaccgatga aggggatgcc aatgcgtaga
      241 tatgtaacca aacgcctctt gtaagtggta acaaatctgt tccgtcaatt aaaggcagca
      301 gcgcggctcc cagtttttgc cgcatgcaat ttcttgtggg cctttttccg gattgttcct
      361 tcgtcaatcc ccaggacctt gacgatgctt cgataggaca gcccctctga acgcatcttc
      421 gcggcccggt cataaacatc ttgcggtatt ggcggccggc caagccgctt gcccttgctc
      481 ctggcgacgg ccagacccgc aacgaccctt tcccggataa tgtccttttc aaattcggcc
      541 acggcgccga cgatctggaa cacgagcttg cctgtaggtg ttgacgtgtc caggttgttg
      601 tcgtaggaca caaagtcgat gccacacgcc ccaagatcgt ccagggtgat gatgaggtcc
      661 ttgagggacc ggctcagcct gtccagcttc cagaccagaa gaatatcaaa cttcctcttc
      721 ctggccgcct ccatcatggc ggtgaacgca gggcgcttgg tgttgccccc gctgtaattc
      781 tgatcaatgt attctttgta aatgttccaa ccggaccggg ccacaaattg acgcagttcg
      841 tgaagctgca tatcgacctt ttgcctgtcc gtggagacgc gggcatagat ggctacggtt
      901 tttgttttcc ttcccatctt catgaaattt tcctcaaaaa catcagggga tcttccgaaa
      961 aaacgggaaa aatttccggt gtaataatca ctgggtttac agagggatgc ttttttataa
     1021 aggcatctaa ccccctggag gatttccggc gtcccgactt tacttctatt gcataaatct
     1081 ttccccggta tttgtagata aagtcaacct ccaggttttt ctctcgccaa taataaagat
     1141 ttcccgcaag ctgctgtagc tggcttccaa tagccgtttc aaaaatccgc ccccgttttt
     1201 cacggtcttc aagaaattga ggcccttcgt tcatggcata cagggcaggg caggacacga
     1261 taatttttgg gctcgatccc cttgtcagat agttcttcac agaatatttt tccaacggat
     1321 ggatgagaaa agctcctgaa tagagctcaa tgtaatactt aaccaagtct gtgttgccct
     1381 tgtcctgtaa ttgaccaagg agcttggtgt agctgatttc ttgagccgga taacggcaca
     1441 gaatctcgaa tgcctgccgg aacagggcag gattgcccac cctcctgttt agcaggatgt
     1501 cttttccgat caccgcttca acgatagagt cttttagata tgcgtaccaa cggtcaaagt
     1561 catcttcata ggcaacggcg ccgggatacc cgccgtaggt tagatagcga tcaaggtcgt
     1621 aaccaaaggc gactttctgt tcatggaacg tccaatggtg agcctttatt agctcaaaac
     1681 gtcctgcaag gctttctgtt aagcctttct gtaattgcag tgaactcgat cccaggacgg
     1741 ctacacgaat agattgggga tctttgtccc ataatgactt gagcgtgtcg gaccagttcg
     1801 gcaccttctg aatctcgtct atgacgagaa gggtctgcct tcccaaggat accgcctttt
     1861 gccattgttc aagcagccat gttcgatccg tattcaggag atcatccgca ttggcataat
     1921 ggctggcata agggtatcgg gccaggagct gcttgacccc cgttgttttc ccaacctggc
     1981 gcggtcctac caagacctga atgcgcgggc tttcggcaat tagtcgctgt tcgagttgag
     2041 aaacaaatga tcgttcatag gcattactca tggagacatg ataagccatt ttgcaagatt
     2101 tgtctatcga aaataatagg cgagacttcc gtttcagatt tctccccttg cacacaaatc
     2161 gctaaaaaag ggttcatccg attcttgcgt acttcttgaa aaggttgcag gcctgtcatg
     2221 gttctttcaa aaggccaagc accaggcaca ggctgaaaaa gaccacggca aatccaataa
     2281 tgttaaaaag aagaacatgc tttaacggcc cccgctgctt aatcccttat aatcaggcca
     2341 ctggtttctc tcgaaatact gtgcggaaaa aaacccttaa atttgttttt acaaaggcct
     2401 taatttttca ggctttttat tttagaaaac ttcagcggat aaatcaaatg ttttatccgc
     2461 tgaaggcaaa tcggtctata tttccagtgc tatttaataa aagagaaatt atagtgcatg
     2521 aaaacgcagc tatgttacag ttggccctgg atagagattt attttctgaa accatattaa
     2581 acaaggcaac cttttgcagt attactcggt aaataaaacg gaggattttt tcaactacaa
     2641 aaagtacgca tcaatcggat gaacccaaat aattaggttt catcaaaaac cttaaaacaa
     2701 ccaagataat atttccttga tttttctata cttatattga ataagaagac aaaaaaccct
     2761 gctaataaaa aacataaagc agctctctga tttgcggagt tctcatgagt tccgaaatag
     2821 aaaataagaa gagcaacgag aagaaactaa gacataggtt ttcctcctgg tggcatcatt
     2881 atccagctat tgagcgattt actctctctg tagccgtgtt caccgcattt cttgccgttt
     2941 ttaccgcatt tcttggagtt gtcggttttc tccaatggtg tacccttgaa aaaaccgtcg
     3001 atacatcccg catacgtgat cgagcgaatg tttatttcag cacgcctgtt tttgaaaagt
     3061 atccacccac gggagaagcc aaagaatata tctttaagat tctcatggca aattctggag
     3121 ccatacccgc gagaaaactt catgttagat acggtgttat tgttgctccg acttccaaca
     3181 ttgattttga gccgtttgac agggttaaat ggataaatgt taatgtgcca atgactcttg
     3241 ggccaaaaca accatttctc ttccaactct ttaaaataga aaccaaattg gctgagtttg
     3301 aagaaatgaa gaagggtaaa gtggttaaat ttatcgttac cgaagtcact tataatgatg
     3361 acttttcctc cgagcggaga cagcgcatta cgcaaatgaa tgtaaggctg aatgttgatg
     3421 gtaatggtga ccatagtttt tcatatttag catctcacaa ttgcaccgac gacgattgcc
     3481 aagtgaggga gggggttgcg acgtcacaga caccatcccc aggaccattc ccatcggatc
     3541 aggaaagccc ctcgatgagg tctttaagaa tggcgtcggt cagcagctct cggttgcccc
     3601 agtcagccgc tccgaaaacg cggtaaaggg tgtcaggatt ggccctctca atttcacgca
     3661 tcgcacgcga tagagcagcg ccaatcgaca attgaccgac ttaacagatt atgtaagatt
     3721 gctaaaaaaa tcgggaaagc caattttgta aattaaatgt tggccggaag gcggaatatg
     3781 agtgctgatc tgaggcaagc tcgatgacct ttattgacat acttaatgac atacgtaaga
     3841 aagcgttctc cgagcaggac aagggcaagc gcttcgagcg actaatgcgg tcctatctgc
     3901 tgacggaccc cctctacgca aatacgctgg aatccgtgtg gatgtggtcc gattttccct
     3961 tccgaaacga cttcagcggc aaggacaccg gcattgacct cgtcgcgcgc acaacggctg
     4021 gcgatttttg ggctatccaa tgcaagtgct atggagcaga cgcttacata gacaaatcag
     4081 gagtggacag ctttctttca acgtccggca agcagtttcg gaacgagagc cttgaaaagg
     4141 tttcctttgc ccaccgtctt tggattgcca ccacgagcaa ctggagccaa gaggcaagca
     4201 aagttctgct gaatcaacag ccgccggttt cccgaatcag cctttccgac ctttcaaacg
     4261 cccctgtgga ctgggacctg ctgatgcagg atcgcatcgg gaagacctcg cgactgacga
     4321 cacacaaggt ccgcaaacat cagcaggacg ctcttgacgc agcgcgtgac cactttgtgc
     4381 aacatgaccg gggcaagatg atcatggctt gtgggacggg taagaccttc gtttccctgc
     4441 gtctggccga gcaggaaacc ggaggtagcg gccttgtcct tttccttgtg ccctcgattt
     4501 cgctcttggg acaaactctg cgcgaatggt cggcacaagc aaacgaacct ttaaaggcta
     4561 tctgtgtgtg ctccgatgcc gaggtcagca aggcccgcaa acggacatcc gatgattctg
     4621 ccgaccagat tgaggacttg gccctgcctg cttcaaccaa cgtcgccagc gtcgtttacc
     4681 agctcgagtc ccatgaacag gacaaacgaa agagggggct aaccgtcgtc ttctcaacct
     4741 accagtccat tgatgtcatc tcgaaggccc aaaggaataa cggacgcccc ttcgatctga
     4801 ttatatgcga tgaagcccat cgaaccactg gcgtgactct cgaaggagac gacgaatccg
     4861 ccttcgtcaa ggtccacgac aacgcattcc tggacgcccg caagcgcctc tacatgaccg
     4921 ccacaccgcg catctatggc gaagtcgcca aggccaaggc ccaagagatg gatgcggcca
     4981 tctgctcgat ggacaacccg gatttctacg gcgaagagtt ttaccggatc gggtttggca
     5041 aggccgtcga aaaactcctg ctttcggatt acaaggtgct catcctcacg gttcacgagg
     5101 acagcatccc cgcagccttc cagaatgcgc tcgccgcagc cgaaaatgcc aaggaaatcc
     5161 ccgccgacga tgtcatgaaa ctcgccggct ccatcaacgc cctgtccaag atcaccatcg
     5221 ggcaggatac gtcctctttg cgcgcaattg accccgatcc catgcggaag gcggtcgctt
     5281 tctgtcagag catccgggca tccaagacca tcacgaacgc tttcaacacc ttcaaggaaa
     5341 ccctttacga acaatacgac ccgtccaccc gccttcagct tgttgatgtt tccgccgatc
     5401 atgtggacgg caccatgtcc gcactgaccc gcgacgcaaa actttcctgg gtaaagtctt
     5461 cacctgcgag cggccgagaa tgccgcgttc tctgcaacgt acgctgcctg tccgaagggg
     5521 tggatgtccc gtcgctcgat gcggtcttgt tcctctctcc ccgcaactcg caggtggaag
     5581 tcgttcaatc cgtgggccgt gtcatgcgca ctgcgcccgg caagaagtac ggctatatca
     5641 tcatcccggt cattattccg tctggcaccg cgccggaaga cgccctcgac gacaacgagc
     5701 gtttcaaggt cgtctggact gtgctcaacg ctctccgtgc ccacgatgac cggttcgacg
     5761 ccatcattaa caaaatcaac ttcaacaagc gcctgcccga tcagattcag gtcatagaca
     5821 gcgccggcgg ctcttccgct gggcaaagct ggtccgacga ggaacgccgt cagttcgagg
     5881 ctgccgttga cagaaagagc attgctgatc tcagtgcgct atgccatgtc atcttcgcta
     5941 agatggtcga gaaggtcggc aacaagcgct attgggagca atgggcgcgt gatgtggccc
     6001 atatcgcaca gcgccatatc gagcagatca ccgccttcat cgcccagggt ggcccgcaga
     6061 agtcagcctt cgaccgcttt atgaagggtc tgcaccagaa catcaacccg tccgtcaccc
     6121 cggaagaggc catccagatg ctcggccagc acctcatcac caagcccgtc ttcgacgctc
     6181 tcttcgagaa ctacaccttt gtcgagaata accccgtttc caaggctatg caggggatgc
     6241 tcgatcagct tgacgctctc accctcgaca aggaccgcgc cgtcctacaa gagttctatg
     6301 aatccgtcaa aatgcgtacc accggcattg acaacggtga agcccgccag aagatagtgg
     6361 tcgaactcta cgacaagttc ttcaagaccg ccttcccaaa ggtcgtcgaa aaactcggca
     6421 tcgtctatac ccccatcgaa gttgtggact tcatgctcca atccgtggcc atcgtgctca
     6481 aagttgaatt cggccgcgaa ctctccgacg agaatatcca cattctcgac ccctttaccg
     6541 gcaccggtac tttcatgacc cgcttgatcc agtccggttt gatcagcaat aaggcgctcg
     6601 accgcaaata caccagcgaa ctccacgcca acgagatagt cctgctcgct tattatatcg
     6661 cctcgatcaa catcgaaaac gcctatcacg aacgcaacct cgacaagccc tacaagccat
     6721 tcgagggaat ctgcctcacc gacaccttcc atctcggcga gggcgagagc aagttcgatt
     6781 ttcaggacgg ctttgtggac aacacccgcc gggttaaagc ccagcgcaaa gcgccgctcc
     6841 gtatcatcat tggtaacccg ccctattctg tcggccagaa atccgccaac gacaacgccc
     6901 agaaccagcg ctacccgagt ctggaaagcc gaatcgagca aacctatgtc gccgccagca
     6961 aggccaccaa caagaactct ctctacgact cttacatcaa agccttccgc tgggccagcg
     7021 accgccttga tccccaggga ggcatcgtcg ccttcgtctc caatggcgcc tggctcgacg
     7081 gcaacgccat gacaggcttc cgcaagaccc tccagcagga gttctcatcc atttacgtct
     7141 tcaacttgcg agggaaccag cgcaccagcg gtgaactctc ccgccgcgag ggcggcaaaa
     7201 tctttggttc gggctcccgc acccccatcg ccatcacttt gttggtcaag aaccccgcag
     7261 cgaaaccgcc ggccacaatc cgctaccatg acatcggcga ttatcacacc cgtgaacaaa
     7321 agctggagat catcaaatcc ttcggttcca tcgccgccat gcgcgacaag ttcaagattc
     7381 tccatcccaa caggcacagc gactggatat cccaccgcga cgaatccttc gaggaactca
     7441 tacctcttgc ccccgaaaag aagttcgaca gcaagagcca aagtgtgttt ctaacctgct
     7501 cgaatggtct taaaagcaat cgtgattcat ggatttacaa tttctcgcaa tctcttttgg
     7561 ataccaacat ccaaacttcc atcgaattct acaacgccca ggcgcaagcc tttgcggagg
     7621 caaggaaaag caatccgtcg atcaagatcg agaactttat tcaatacgac tccacaaaat
     7681 tcagttggaa tcgtggttca aagaacgatg cggctaaagg taagcaatac cgttttgagc
     7741 cggagtctat ctgtactgcc ctttatcgcc ccttctgccg tcagtacggg tacttttctg
     7801 ctcccatgaa tgacatggtt taccaactcc ccaaactctt cccaaccacc aagcacgaaa
     7861 acctgctgat ctgcgttccg ggtatcggag tgaccaagga ttttgattgc ttcattacag
     7921 attgcctgcc ggatgtccag ttgatggcta atggccagtg ctttccgctt tacttctacg
     7981 acaagaacga gcatgttcag caggaaagcc tgttcgatac cggcgagaaa tacatcaggc
     8041 gcgacggcat cagcgatttc attctcaacc gtgtacggga actatgcggg ccgaaggcca
     8101 cgaagaagaa tatcttctat tatgtctatg gtatcctcca cagcaaacaa taccgcgcca
     8161 agtttgcggt gaaccttaag aagtcgcttc cacgcctacc tctgccggaa gaccccaagg
     8221 ccttctggga acttagcaag gccggggtgg aacttgccga actgcatctg aactatgaga
     8281 ctctgccgcc gcacaaagca atcatggaag acagcccgaa gaaggctccc tcgacccgcg
     8341 tccagaagat gcgcttcgac aaacggggga atgaagagga caagaccacc atcatttaca
     8401 acgaggacat cacgctccgg aacattcctc tcgaagccta cgactacgtg gttaacggca
     8461 aatctggcat cgaatgggtg atggagcgct atcaggtcac gacgcacaag gaaagcggca
     8521 tcatcaatga ccccaacctc tggtgcgacg aacacgacaa tccccgctac atcctcgacc
     8581 taatcaagcg ccttgccacc ctatccttgg aaacgcggcg aatcgtcaac gccttgccag
     8641 ttttggacct ctgatggata ggggaatggg cggcagatat tacaacgtga gattcgcacg
     8701 taaccataac agccagctat cggcttctgt gtacgtcgct tacgcaccgc tgtgcctcac
     8761 cctgtacatg tgcgacagaa gcatgaaaaa gccccccaac gtttatgccg gacgctggaa
     8821 ggggtggcgg aatcagccgg aatcccatgg cgctttgcat cggaacaggg tggcggttcc
     8881 ggattcgagt ggagtaatca gcggaatacg cagcgtcacc aaatagcgaa ccggcgctac
     8941 actaaattct tgctggaggt gtcttatttt cattgacaag ttccggtccc cacattgagt
     9001 tttgctgtta tgacaaagaa attaaattgg gtttaattta aagatgctga ggagaatcaa
     9061 gtaatggctg gatcgacatt tcaaactaac ccttatgact tgagcaagct tcttgacgac
     9121 tgtcaccagg gagttcttca actagggcca actgtaacat aactgtgttt tcacgcaata
     9181 gaatgatttg gcttatgttt ttttaatttg atatttcttg gttgcatgaa tgttgggttt
     9241 aggcctattt atcaggatct gagtctggca aacatagaaa aggaatacag gaatgcgatg
     9301 aaggatttcc ctatccagtg aattggaaat gtagttccag ttctatttta aaaaaggaaa
     9361 ttatagtgca tgaaaacaca gctatgtttt catagggccc aagaggagcg aagcttccag
     9421 gaacagctca atcagaaggc tcatgcctat cccgacatgg tgccgctgct tgtgataagg
     9481 attgaatgaa tgcataaacg acaagatgtt gttcgacatg agggcggtta cttagttcgc
     9541 gtttttcaat gcattgacgg atttcgcgct cgatacggac agtggccgac aaaactcaat
     9601 ttggggaaac tggcgtatga aggtttttat tcccatttaa ctgagtttgg attggggtta
     9661 ataaagagta agattaatat tgaaatcgat tacacagaaa tagaccggat atccgcggca
     9721 gatgattcaa ataaccggct cgattacggc tggtatggag aaagaccgga tatagatgct
     9781 tatcagtggc tcagaataga ataagctaaa tgggatatgt aattcactac caaaggatat
     9841 ggaccaagtt tgataatgat gagtagctgg cgcgatcaaa tcctaaggga attcaaaccg
     9901 aaagtcgccc gacttaccct ggtggccgat ccggacgggc ttctcctaga agggccaact
     9961 gtaacatagc tgtgttttca cgcaatagaa tgatttggct tatgtttttt aaatttgata
    10021 cttgattgca tgaatgttgg gtttaggcct atctatcagg atctgagtct ggcagacata
    10081 gaaaaggaat acaggaatgc aatgaaggat ttccctatcc aaggataaca atatattcca
    10141 ggtctattta ttaaaagaga atttctattg cgtgaaaacg cagctatgtt acagttaccc
    10201 ttttataggc tttttcttgg ccaagacgtc agtaattgag gcggtaaagc tctccggttc
    10261 aattccgagg ccaatggttg aatgggcttg tggatccaga tcaatcaaaa gcgttttttt
    10321 gccttttctg gccaaaccag cagagaagtt tattgacgtg gtagttttgc ctacgccgcc
    10381 tttttgattg atgatagaaa tgatgttttt attcataaca ttaaggattt tttaaagtgt
    10441 catgggtgcc aatggttctt aaaatataaa aatcttccgc tatctgaaag gtaaatcggt
    10501 aactgtgtgt taccctgcat tcccaaatgt tccttggatc ttgcatcttc tttgtgttta
    10561 atgatggatg ttgaggattt gtaagcaaaa attccagctg cttttttgct agttcttgaa
    10621 ttgccgtagg tagtttttga tattgttttt taaatttctc ggttgggtat agcttcatta
    10681 tttttaaggt taactatcca tggcttttgc gaaatcttca acgttatcga aagggccaag
    10741 catcctgcct ttggcaatgt ccttgtccgc ttctttttcc ccgtcctgcc attccttggt
    10801 ataaaaatat gccaagtccg attgcaccac tttgacaggc tttaaaacca tattgccatc
    10861 tatttcttca atttccaaat agtcgccttc atcaatgtta aacttctttc ttataagaga
    10921 aggcagggtt atttggcagt accttttcac ctttactaag ggcatatttt ttacctccgt
    10981 gcgttacttc atgtgttaac aatttcaaat taaaaatatt tgaaattcac gttttcataa
    11041 taccatagct tgggagtttg tcaagccact tttacaaaac atagacatag gaggacacag
    11101 atcatgaaaa tgaaaaagac attaggaacg atggtagcgg ctttggcagt ggttcttttg
    11161 accggaactt tcgctttagc agaatatgca gcagccggtg tggcgaactt cccttacttc
    11221 cagcttggat gcttgatcat tggcgggatg accgtaatca gcccatttgt ccataacttc
    11281 ctccttaagt cttaagttgt cactaataga tcaaatgatc ctatattgct aatagtttag
    11341 caacttcttt gcctaatttg actgctcctg tgcccagacc ttccagtttc tgctggaatt
    11401 cagccaaagc cagattattc tgcgtcgctt cagtaattat tgggatcatt ttattatata
    11461 ttgtcggatc actgaccttc ggcctaagtg aatcgagatc ccttttcgta gcttgtttca
    11521 aagaattaaa ctcatctaat aagaggccat ctgtttcctc gatggattcc tgcgaagctt
    11581 tttcaagatc atctttcaga gacatgatta tttttctcct ttgattttat ttatgattat
    11641 ttgtttcttt tcatcaatat ctggaatgat tttttccgct ttcgccactt gttcaatcgc
    11701 tgatgttgcc ttatttgatg ccgatataag ttgattatta attaggtcac gtaactcctt
    11761 gatattgaga gccttgagag catcgtcctg cacttcctga actttacgta tgctattcaa
    11821 ttgcgccgtt acagcagcat tagctcttgt tatacgggca aatgcatcat caataatttt
    11881 taaaatattt tgttcatcat cattaattgg cgatattaag tctttcttct ttttctcgat
    11941 tgcttttaca gcaatttcag cccacgtact tacaccttca aatgctttca cgggatccgg
    12001 atcttgagca agtgaaatta atttcccttt tttaacgaaa tttccaagat aggttggcgt
    12061 ccattttttg tccaagaaat cattggtttg atttctaaga ttttcaaaat gttggtgtat
    12121 caaatttgcg taagacgtat gcacagcatc aaggtcttgc ccaattgcat atgagagctc
    12181 aactgtttct tttggaattg tggcgcatcc gtaaaagaac attgaaaagg caatcaaaaa
    12241 aatgatcaga ttaaatagag attttttcat ttcaaactcc ttaatcattc tacttttctt
    12301 tccgaacgcc tttgaaggga tcttcatcgg ccttttgatc catgaaccgc ccgttatcgg
    12361 tgtttctctt ggtccatcga tcattccgag gattatacgt ctgggatcgg tcctttactg
    12421 ggccgtgccg gtgcccgtcg ccaatcggcg gattctttgc catgtgcatt tacctccttt
    12481 ttcatttaag ctatacgcta atttagttac tgtatatcga actatttcgg cgttgtcaag
    12541 attttatttc ttgcatttca taaccgtgtt cgtgtataga gaacttaaat gtcacactaa
    12601 agaaggagaa ccaaattgac taccggtaaa agaatcagag agatacgtga aaagcgcggc
    12661 ctgacacagg aagaacttgc caagaaagcc ggaatgagca agggcttttt aagcgatgtc
    12721 gagaacgata aaacgaacat tggttcacag ggcatattga aactcgccaa tgagctaggg
    12781 gcgtctgtag attatttact cgcgggaaga gttgcaaaga atgtcgaaac aaaaaaaatc
    12841 gtgatcccgc aagaactatc tcaggcggcg gaagacttga aattaagcta tagcactacg
    12901 ctggatctgc taaacacttt caactcagct gtggcccgaa ggggtaagac atttcacaag
    12961 gagctgacgg ttgatggatg gaaaaagttt tatgaagcca taaaggaggc gattgaataa
    13021 gtggccacaa gactaaatcg gtcaacaaga atttggagat tagccaagga tttaggtatc
    13081 cgaacagtca ctgatcctgt ttctgacatc ctcaaccttt gcgaaaagcg aatcaaaaaa
    13141 atgttgaagg atgcgactga ctgtcgatcg ttgtcaagtt tccttgactg gatagccggt
    13201 aatctcggga catcctttga ggaaatccgc agtgacgatg attttttaag aattaagaag
    13261 aagtatcttg cgctcgggga aaagatattt gcaacccttg agcaggaatt ttcggatgaa
    13321 gttttgggta ttacataccg cctatcacac cgggaatctt gggaacccca gtttgtgtcg
    13381 gtaattgatt gccgtggagg caattccata agagcatatt atacgaagtg gcatgaggtg
    13441 gcccatctcc tgattctcac tgatcagtca cgcttatgtt ttcgccgaac ggagcatctt
    13501 cccgataaag accctgaaga ggttttgatt gatcgcattg ccgggaaatt tggattttat
    13561 cccccattca taaagccgca catcaaaggc gaaatatcct ttgaacaaat cgaaacctta
    13621 aggcaagctc tgtgtcctga ggcgagtaat ttggcttctc tcatcggctt tgttaaagca
    13681 tggccggaac caggtttgtt ggttctgtgt aaagaggggc taaaaaaatc ggaaagcaga
    13741 cgactatcga ttcaacggag cttcgatttc agtacccttc cggttccggt attacgtgcc
    13801 ataaaagtca ctccgaacga ggcagcaaag gagcagggta tcttaatcca tgaaaatatg
    13861 cgtatacctg agcgttcggt aatctatgcc gttcacaaca gcggcgaagc tgagtctgcc
    13921 gaagccatgg aagatttgtc ttggtgggag agtagcagtg gggctatctt gccgaagcaa
    13981 aaaatctcag tcaaagcaaa agatttctgg ggcgatacct atgccctgat cattccaaaa
    14041 tttagatcaa gatcttgaat ccaggattta atgttcaatc tgtaatttcg aaatctgatc
    14101 gatataaaaa ctacggatga aacattgttt tacccgcagt ggtgagtgag atagccgtta
    14161 acgaacaacg aaagttgtcg gataaccagg cgctgcacgt gcgtctcccc gtgagctttt
    14221 cgttcggtag atagaaataa tatcaagagg gaatcatcgg aggaactatg gagcaccaat
    14281 actatgacct tcatcaactt cgaggtggcg aagtcgttga agtccaattg acgaacgccg
    14341 caaatgttcg attgatggat agttcaaatt actccaacta caaacgtaac ggacaacatc
    14401 gatacattgg ggggtatgtc aaacagtcac catacagaat aacagtccca aactctggac
    14461 attggtatgt tgccattgat ctcggtggac atgcagggcg aattaggtca tctgtaagag
    14521 ttctgcctgg tattttaccc acagcgagta atgcgccact ttcttcaatg ccgtcactac
    14581 ggcttgatag atctgagatc ccagaagaag acaaccgtgt tcgcgagtat gacgtttttg
    14641 tttcacatgc atccgaagac aaagagttag ttgctagacc attagcggag aaattgcgca
    14701 aactcggatt aagggtctgg tttgatgagt ttgaattgaa aattggagat agcctgagga
    14761 gaaaaatcga caaaggcctt gccaacagta agtttggtgt tgttatttta tcgaagtcat
    14821 tcatcaataa aggttgggcg aattacgagc ttgacggaat tataaccaag tcagtatcag
    14881 ggcagcaagt tgttttacca atttggcatg acataacaaa acaagaagtg attgattaca
    14941 gtccatccct tgctgataaa cttgcgagaa atacagcgat cagtacaatt gaagagattg
    15001 cggaagaaat caacgaggtc gtcaggtgat atatcaatga ataattgcat gatgaatctc
    15061 aaaaaaatca taatgataga attgcaaccg aacccaatcc agccgatcgc tacgctccgg
    15121 ctgatttcgt cgttgggcag gtagagaatg atgagcatgc ataggcaaga accccatgta
    15181 ttcatttcgt atttgcggga agaccgcgaa gatattgacc gcatctgcga tgccctgaag
    15241 cgaaaagaca ttcaggtctg gatagatcgt gaaaagatcc tgccgggaga acgatggcag
    15301 atcgccataa ggagagcaat cgaggagggg gcttacttct tggcttgttt ctccgatgca
    15361 tacgccaaac gtgaaacatc atatatgaat gtagagctcg cgatagcggt agagcagctc
    15421 gccttacgtc cgaccgatcg ggcttggttc attcctatag tattgaaggg tgggcgcgtc
    15481 cccgaccgcc caattggagg cggagaaacc ctcagggacg tccagtgggt cgatcttgct
    15541 gaagactggg aagaaggact cagacgcata ctgactgttc ttcgacccga ggcggctcat
    15601 agaaagggag cggtggaacc tgagtacgcc gttattctaa tgacggatat cgtgggctcc
    15661 agccgaattt cccagggtct cgatcaagac ggtttccatc gaaccatagt ggatatcagt
    15721 ctgtctctta tggagtcagc agctgaacat ggcggccgtc aggtagcgtc tagtggtgat
    15781 ggaatggttg caaccttcga ttctggtacg atggctttac gatgcgcgct tgcgatcaat
    15841 cgctttgttg gaagctacaa ggtgaatgaa gaaccactga agattcgaat tggccttgcc
    15901 gagggcaaaa cattcgatac cgacaagggc tttgagcaag atgctcatca tgtggctgct
    15961 cgaatctgca gtgcggctgg tcctggcgaa atcttctgca cagagtcgct cggaagattg
    16021 gccgcaagtc agaactggga ataccgagac cgtggatggg tcagcctgaa gggctttgcg
    16081 gaaccaaccc acatctatga gataatatct tgatcttctg gaggtcgtgg cagcagcgta
    16141 tgttggcgta agcccttgac agagaacaag ttgaagagcg aaaagcattg cccaactaag
    16201 catcggagcc gacctcgtac ctcggcggct caactcgccg ttgggcatca gaattaaaac
    16261 attatctcgg gtcctattat gagtgatcaa aaatgtccaa tatgttcatt gatcgtggaa
    16321 gaaactggta gaggcggaag cgctgatttt catgactata agtgcccggt atgcggaccg
    16381 tttactatca cacgcaccgc tttgcataat ctaagatcaa aaacattaac tgagaagaaa
    16441 tcagcggttc ttagccatgc tatacggaga atgcaaaaac aagattcatg gccagagatt
    16501 aactcaaaaa tactcgacgt tataatggaa aataattatt tgccgtcccc tgcagaacaa
    16561 gccgacaatt tgattctatg gataggcgat aatgtagagg tccctggagt tgaaataggc
    16621 gttgatcctg atttgcataa agcgattatt ggagccgtta acaaccataa tttccacttt
    16681 attgtaagct cactagtaga acagggcctc gttcttagca agagcgaaat ggttaaacgg
    16741 cccgtaaccc tttcttttag aggttggcag cgttatgagc tcattaaaaa aggtgcttta
    16801 gacagccgca aagcgtttat ggcaatgcct tatggcaatg atttgatcga taaagtctat
    16861 agagattact ttaaaaaggc agtcgagcaa acaggtttta atctattccg aattgatgag
    16921 aaaccgaaag ctggctcaat tgatgacagg cttcgtgttg aaattcgtac ctctaaattt
    16981 ttaattgcgg aactcacaga aggaaatcat ggagcttatt gggaagccgg atttgcagag
    17041 ggccttggta agcctgttat atacacgtgt gaaagtactt actttaagaa caaagggaca
    17101 cattttgata caaatcattt gcatacggta ttatgggaac gtgaaggact agaggacgca
    17161 gcggagcgac taaaaattac cattagggca actttgccag aagatgcaaa actaaaagac
    17221 gactgatttt agtgttgccc aacctgtcaa tggagaggga cgtgaaaaac actgacgcgt
    17281 tttccccgcc cctcatttcg gcgttacacc tggaatggac aaaaggaatg attgacatac
    17341 agacaaggaa gaagctgctt taagtcttgt tgctgaatct tttctgtctg caaccgaaga
    17401 gcaagtcaat gagctattgg ctaacggctc gatatgggag gtacaacatc atgagtgaaa
    17461 tacttgacaa tgcaaatgaa atggcacgtg atctgttcaa ggttggtgcc atggatgaaa
    17521 ttaccatgcg caagatagac gcactttgcc ttccaaccaa acggacattt cagcctgaag
    17581 acatccggcg cattcgatct gctaaccatg ttagccaagc cgtgttcgct gctgttcttg
    17641 gcattggcaa gacaacagtg caacaatggg agcagggaca aaaaaaacca agtggcccgg
    17701 cacagcggct tcttgatctc attgatcgta aaggactggt cgtactcggc taaaataagc
    17761 cagagaaggc attgcttaag attgaggcaa tttgctgatc tcatcctgat tgctctgctt
    17821 tacaaacctc aatcaacaaa atccaggcac ttctcaacaa tctttattga tttcctattt
    17881 tcccgtaaaa atcaatgcat ttcgaaataa gtatattcct cccgaaaact gcttgcaata
    17941 tcaaaacaat ccatataata ataggaacgt agtccccaat ttatagttaa tgtgggtgtc
    18001 attatgatca aaagagaaat ccaggcagtt atcgagcaaa ccttgttcaa agggaaggca
    18061 atcatcattt acggtgccag gcaggtgggc aagaccacgt tgatcaaggc tatacagcag
    18121 aacgccactg ttccctcgct ttacctgaac tgcgacgagc cggacatccg ccgggcgctt
    18181 tccgacaaga cctccactga actgaagatg ataataggca ataacaaact ggtgctgatc
    18241 gatgaggcac aacgggtcag tactatcggg cttgccatca agctcctgac cgacacggcg
    18301 ccggatctcc aggtgatcgc cacgggctcg tcagctttcg agctttccag ccggatagaa
    18361 gaaccgctga ccggcaggaa gcgagaattc cggctttatc ctttttcatt gatggaactc
    18421 ggccagatgt actcgcccat cgaaatccgg cgcattgtgg agcggtgcat ggtttacggc
    18481 ctctatccgg agattatcaa cgccccggag gcggctggca cagctttgcg ggaaattgcc
    18541 aaaagctatc tttacaagga tatccttacc tttcaacagg tgcggaatgc cgaagcgctg
    18601 gagcgcctag tgcagtccgt cgctttgcag accggcaacg aggtctcata caacgaactt
    18661 gcccagcagg tgggcgtgga caagaagacg atcgagagct atctgcgcat cctggagcag
    18721 tcgttcatta ttttccgttt agggtcgttc agcaggaacc tgcggaacga actcaagaag
    18781 tcacgcaaaa tctatttcgt ggatacgggt gtccgcaatg ccgtcatcaa caatctcaat
    18841 cctccggacc tgcgcagcga cacgggcggc ctgtgggaga attttgtcat tgccgagcgg
    18901 atgaaacgga accacaacca gcaattgttt cccaacacat atttctggcg cagtcaccag
    18961 agacaggaaa tcgattatct ggaagagcaa aacggcgagt tgcgggggta taaaatcaag
    19021 tggcgtgaga ccaggttgaa gattcccgcc ggattcagcg cggcctaccc tgattgcccg
    19081 gttagcctgg tcaatcgtga aaatgtggcg gagtttgtga gatgatcgga cacaacccga
    19141 tgatcataaa ggaagcccca tgatccgtgt catcgtaagt gaaaaaccca gtatgggccg
    19201 ggccatcgcg gcggccttgg ggatccaggg aacgggacgc agcttcatcc agggcaagga
    19261 cgtcatcgtg acgtggtgcg tgggtcactt ggtccaggct atagaaccgg agggatataa
    19321 ccccgatctc aaaacgtggc gcatggatac gctgccgata ttccccgacc agttccgcta
    19381 tgcgcccatc gaggccaccc gggaccagta cgcggttgtg gcgagtctca tgaaccggga
    19441 cgacgtggcg gatgtagtta atgcgacgga cgccgggcgg gagggcgagc tgattttcga
    19501 tctcgtttac cgcctgtctg gttgcgccaa gcccgttcag agattctgga cgtcgagcct
    19561 cacggacaat gccatccggg aggcctacgc ctccatgaag ccgggcaagg cctactacgg
    19621 cctgcgggac gcggcgcgca gccggcagga atcggactgg ctggttggca tcaactgcac
    19681 ccgggcccag acgctggtca tgcgacggtc cggaggggaa ggggtatact ctataggccg
    19741 cgtgcagacg ccgaccttgg cgatcctcgt caatcgtgaa ctcgaaatca cggacttcgt
    19801 gccgaaggaa ttctggaccg tatgggccgt tttccaggcc caggagggca tataccgcgg
    19861 caagtggttc cataaggtgg acgggaagga tcaggaccga ttcgaccggg aagaggacgc
    19921 gaaggacatt gtaaacaagc tgtccggctt gcccggcaaa gttgtctcgg tcatctccaa
    19981 gacggagaag aaaaagcccg aactgctgta cgatctcact actcttcaga aagaggccaa
    20041 ccgacgtttc gggtttactg ccgaacatac cctggaggtg gcgcaggagc tctatgaggg
    20101 caagcttatc agttatcccc gcacgaactc ccggtacctg acggaagcgg acgcgcagaa
    20161 ggccacaggg tggatcaagg ccgtcgccca ggggcagctc atggatctgc aacccttcat
    20221 tggggagctt cggaagcgct ggccggtgaa gctcgacaaa cgcttcgtga acgataagga
    20281 ggtggaagat catgcggcac tgacgcctac ggagaatccc gcggggaatc tccatggtga
    20341 cctgctgaag gtatacgagt tgatcgcccg ccgcttcctg gccgcgtatt ttcccgaccg
    20401 catcgaaggc aagaccacca tcatcacgaa gatcgccaag gagaccttca agaccacggg
    20461 cacactagtg aaggaattgg gatggtccgc cgtcgaccca ccccatggcc gtccgaaaaa
    20521 agaaaaggcg aggacaaagg gccctgcgga tgagattgag gaggacgagg atacgggcct
    20581 tctcccttcc gttgtcaagg aagaaaccgt ggatacaaag gacatcatga ccagggccgg
    20641 caagacctcg ccccccaagc gcctgacgga aggggacctg ctgggtgcca tgcagagcgc
    20701 gggacgagaa ctggacgatg aagaactgaa gggcgccatg aaggactgtg gcctgggcac
    20761 gccggccacc cgggccaaca tcatcgagac gctgctaaag cgcggcttcg tcgagcgcat
    20821 acgcagtgtc ttgcaaccga cggccaaggg gatcgagttg atccaatcca tccaggccga
    20881 gacattgaaa agtccccaga tgaccggcga atgggaagcg cgcctggaga ggatccgccg
    20941 gggtgaggcc aagcgggacg agttcatggg tgggatccgg accttcgtga cggaacttgt
    21001 ggggcagatc gagcaacagg tgccccagca gcagcgcact tttggcgctg tggtgggtac
    21061 ctgcccgaag tgtggatcca acctgctgct caggagctgg gaagaccgtt actacgtgaa
    21121 gtgcgctgcg tccgcgaatc ccaaatgcca tgtttccttt gatgcgaatg ccgaagggaa
    21181 agctctgaag ttttgcatgt tctgccgggg cccggtccga accacccgga acgggggaaa
    21241 agtctgcgct caatgcaata gatggcaatc aagcagaaga aagggctacg ccgcttcggg
    21301 taggtcctct gcaggccctt ccggtagcct tttttagtga tcaactcaag ggatgtgggc
    21361 aggacgctgg tagaaacact aggttcatcc ggctgatgca tactttgggc aaaaatcgca
    21421 ttgttgacta ctcgcttcca tctacgagtc aacggaaacg agagccatta ataactgaag
    21481 attaacgatc gaaagagagc aatgcaaagc ttaatgcgag accattttca ttgccgagag
    21541 gtaacgttga acagttggcg ctaaagctct ccagccaaga ggaacaccgg ccagtttcgc
    21601 cgctgcaccc gatgaaagct tgcccatttc gaaaagcttc acagctgcag ccatgcgcag
    21661 cgttgcgcct agttcctctc tcggggaatc tttaaggtaa tcaaaacatc ttcctataat
    21721 cccatctttg ccttttttgc tttcaagtta ggagtgacca taaatggttt ccctggttgg
    21781 catgtacccc gaaagtcatt ggtatatatt aaacagattt atcatcatgc agtatatata
    21841 ttatcatgga gttttacgaa ctgaatgaag ttgcaaagtt ggcattattg accccaataa
    21901 tacaaatgcc ctctggttat acgattgtta ggtttaccat catttgatgc agggcggcgc
    21961 gcaccggcac tttacctgta ggatgctgcc gaaaaaccat agtatagatc attgtttgat
    22021 ccttgagtag aaacgttcca gcagtcgggt tttgcccgat tctttggcat cgctcatccc
    22081 cacgtaaaac cagaaccctg gcgcagtatt gaacataaac tcaccatcca ccagggcagc
    22141 ggtccatccg ttcttgttca gggagaggga gtcctttatc cggatgctgc cggagacgtc
    22201 gtccttcgag gtatagatgt tgagagactt gccgggctcg gcctttatca tattgaaggc
    22261 cgcaagcttg aatctgccgg gctgtcccgg cttaaggcgt tccaattcct ttctgctcag
    22321 cgcgttttcc gcaaccagac ttaacaattc ctcaaaagaa agtcctttca ataatttgag
    22381 tgcgggtagc ttgtttgccg gcaggattag gaaccggata tccgcaccgt caggatagta
    22441 aggaatcgcc acggcctggc ggatcagcac cgccttttca ctgatgatat agtccgcggc
    22501 aataacggtg aacatttggc ggccaatgct gtcggctatc ttgtagactg cttcaatgcg
    22561 ccggtgcagc gggtcatttc ccacctcctc gtcccggcgg atgctgatgc gctgcaagac
    22621 aaatccggac agatcaatct cagtctccac gtcccggcgc aggtcggaaa ccgaaggatc
    22681 aaaggccaca acctcggggg catgcatctc ccgaccctga agtcggtaga agagcgcctg
    22741 gagcacggtg gaaagatcga ggcggacctg gcccagaagg gcgggcatgg ccggatcatg
    22801 cgccggcgca gtactaggct gcttctttgc agtgatgccg gcgctggtgg cgtcagtgaa
    22861 agacaggcag accgataatg gggaggagaa aagaaggaat gagacaatta ccggcagggc
    22921 agacaaacgc tttttcattt acaggactcc tttataaccg gttcaatgac tcaaatccgg
    22981 gaagctgagg ctgtgcctga ccggttcggg gcggcggttt caatgtcttt tcctcggtca
    23041 tgcctttggt aatcatgttt tcgcctgcgg tggcgatccg tttgtttgcc tccttggcta
    23101 aggctttgag accttctgtt ggcgaccggg agagctccat ggtgttgaca aatattttat
    23161 tctttaacgg ctcggaagca gcgccagctg cgtttttctg ggccgcacgg agggccagtt
    23221 tgccggtgac agtatcgact gcagcgccgc cgatttttcc ggcagtgaaa tccaccagag
    23281 aggccttggc ggcttcgtac acggcttttg aagggtcacc gcttttccag aatgcttctg
    23341 ctgttgccgc ccccacccct tcggcaaggg tcgaggcaag atcaaactcc gacatggtaa
    23401 gtgccaaggc cggacatatc gtacccaggc tgtaaagaca tcccgtagcc aacactccaa
    23461 cttttgccag atcgatgccc agtccggcat aatccaggac cgtttccgtg tagtccccgg
    23521 caaaattgta aattttgtct tccgcagcat tacgctccac aatcccctga tccgtccatt
    23581 ttttatggag cttgatctcg ttgagtaagt cgtttctgga gacaggctcc agttttttgg
    23641 cgatctggat cagaaaattt atttcatcct gctgccggtc aagatccttg ctggaccaag
    23701 catccttcag ccgcaggatt ctcctggcaa caacccgctc gtcaaaggtg aggcgattga
    23761 tattttcccg atatttttcc caggtcccgg caaggaaaac aatggcatcc atatcaattt
    23821 tcttggccgg aatctgggtt atgctttctt cagccggcgg cggtgaaggc actttatagg
    23881 ccagcagtac gggaagaacg gcgatctccc gggccgcctg cgtcaaaacc gtgatcatgg
    23941 ccttggaaat ggctttgtac tcctggctct cattgggaaa gatggtgctg gtaccttcct
    24001 tgatatcctc gacctgatag gtcttttcaa aaatctgcct tccttgggca aataatccca
    24061 cactcatctt cattgtcacg gacatcaccg gagtccaggt gccgccccac ttaaatcgaa
    24121 attcatccag gcggggcaca atcgctacct cttcggcgcc aagcccctgg tggcttgaaa
    24181 ccccccggat atgttcaaac acctggggga atatcatgag acatgccttg tgtgccccct
    24241 cgcccaggtg gaattcgtag gtccggaacg atccctgaaa agaatcggat ttgcccctga
    24301 atatgtaatt gctgaattgg ggggtgagaa ccagggccgc aggcgcagct aactttgtga
    24361 catttagccg ggcatcaggg aaggtggcaa aatccacccg gtcgtacaac ggaacattga
    24421 aggacatgcc cgccttcttt tctgatccct ggataatggt tccgacatct ttggcatcaa
    24481 caccggcatt tttcaattgc tgctcgatgg cggcgcccac tgtctggcta aagacgcact
    24541 cgggggctat ccccgtcaag actgccacaa gcaaaatgat catcccgtaa aaaaggcgca
    24601 ttggctttct cctagatatt cagaggacaa tctggacagg aaattcgagc ggatcaccgg
    24661 tagtaatctg gaacatgctg cccgaagtcc aatttgctgt ttcgtaaaat atccctgctt
    24721 cccaaccaga accacaagcg gacatatgta ccaattactg aaaatctttt ctttttttcc
    24781 gtgtttccgt caggaaggag cccacgggga aagccatctc aaatatcggg aaactccggc
    24841 ggctgcttgc caagaaatgc cgtaatgccc tggatgcatt cacctgaagt aataagtgta
    24901 atcgcctctt ggcactctaa ctcaagggcc tgcttcatcg tgagttcttg tgcctgtctg
    24961 ataactgtca gagcatgccg tactgctcgt ggaccgttct tggctatcat ttccgccatt
    25021 tccatggctt catccagggc catcccctgg gcgctgatgc ggttgatgag cccgattctc
    25081 atggcctcat tcgctccaac cttccggccg gttaaaatca tatcagcggc ccgtgatgcg
    25141 cctatcagcc tagtaagacc cggcccgccg ccccagtcag gcattaaacc cagtttcact
    25201 tccgcaaaac agatcaccgc tcgaggatcc atcacccgaa ggtcacaacg tgatgccagt
    25261 tccgctccgc caccataggc gtcgccattg atggctgcaa tgacgggaac aggcagcatt
    25321 accagatcat tcactgcaga ctgcaaacgt tttattaaaa tttcgactgg gggtcgctcc
    25381 tgcttttgca ctgactcaat cagaccgctt acttgggggt tatctggatt gatgtcgaat
    25441 ccagcacaga aggcttttcc ggaaccagtg acaataatga cactggggag atgttctcta
    25501 agtttcctta ccttctgctc aaggtccgac cacataagtt cgttgaaggc attccggcgc
    25561 tcgggccggt cgaaagttat tagtgcaacc ttaccttttc tctcaaaacg aacaccatat
    25621 tcagtcacac tcagccacct accatttaag ttttacaccg ttgtcgagaa agcttcattt
    25681 ttgaaaagat attgcttcta tgtagagatg aaagtataag aataacggac ttgtatgtca
    25741 agggaaaaca aaatgtggga ttctgtacct ataattgatg catagagagc attgccgctt
    25801 gaaagaaagc actctcgaca caattcatag aagctcgact acaggaagga gtcgttgatt
    25861 gccataactt atcagtttgc ttcagttttt ggctgcaatg caccctatat cctgtcaatc
    25921 agtttgtgag ttccgatata gtacagcttg taaatagatc cttcttgttt gaagatacac
    25981 ctataaccca tgtccacata gaattcccaa aaattagttc cttctattcg atgtatttgc
    26041 aatgacttat gtttgggatt agatttcaag agttttaatt gctttcggaa tttctttttt
    26101 atgtcttttg gctgatcgtc atattgagag aagaattcat cagatacatc aacaatataa
    26161 tcaggcatct tttagaacct taagaagatc atcaacgcct tcaaatcgtt tgaatttccc
    26221 atttttaaac tcttcctcgg atgacttaat cttctgctga acctcggatg tccagaacca
    26281 ggattgtgat cgaggtacgc caataaccgg ctctaaaata atcttcccat ctttcagatc
    26341 aacagaaaga ctgtctcctt tttggaggtt aagaatcgtt ctcaaatctt ttggtaaagc
    26401 gatttgacct tttgcagata ctgtcaaagt tctcatgata aacacccttg tagatttttc
    26461 ttacagtaag aatatgcttc ttcatcgaat aaatcaagct ttgtttgatg aagaccatag
    26521 aatatccaaa caagaaactt ccgaggataa atcatttgat ttgtccgctg agatggacac
    26581 tctttcccaa ctgtcagaca aattatttat gacatactac cttggctcaa gcggcgaatt
    26641 atcacagaaa aagggataag gaaactgcac actttcaacc cccgcgttgg cggggaacgc
    26701 acttttcacg ctttgcataa ccttgaggca atggtctttc aacccccgcg cacgcgggga
    26761 aataaattcg ccggactatg gttgttgttc ctgctcttgc tcggtttcag cctgttcggt
    26821 ttccacctgc tctccttccg gctcctcggc gtgttcggct tccgcgttaa cctcccggtc
    26881 aaactctctc tggcatggcc ggcatactcg taccgcatca ctcgcttgat aaagccgttc
    26941 ctcattgtac agcttgccac agtacatctc tgcttgtccg ttgccgtccc gcgtgcgaac
    27001 gatatgccgc ctcttccctt tctttgtttt ggcgtacagt atcgtctctt ccgtttcttg
    27061 cgtttcttgc attgcttctg tttcttgcgc tccttccatt cctttcttcc tttctttatt
    27121 taatcaaata ttaccttgat tttcaatcca tattccgtcg agttgttctc cggctcccaa
    27181 ccgacccatc ctgtctgata gcgccggtac gtgatccctt tggcatccga cttgtcgatg
    27241 ttccagtacc ggacgaatgg ctccagggcg taggtggccc gcctggtcgc catggcaacg
    27301 tacaacgatg cccgccaccc gtagccgctg ttctgatcgt tatccaattt gtctatgcca
    27361 cgcactacgt cgcccaggtg actcgtctgc ttgcccgtcc aaaaaagatc atactcggcg
    27421 cttgctccgt acgaccactt tttgtttatg gccttcgatg cctcaatgcc gatgggactg
    27481 tatatgtagt tggattcccg tttgtaaccc ttgtggccgg tagtgctggt cttgcctgat
    27541 acatcgtcgc acaggtatcg gtagcctatg cccacaaaag gggaaatggt gaaggtcttg
    27601 ctcggtttga aatcataccc taacagaccc cggacctcat agccgtaatc attgatgccg
    27661 ctgatcgaac cggtatcctg gctgtcgtag tccattctgc tatagctgaa tcttccagcg
    27721 atccttgcca tgatcttgtt gtggtacgtg tacgacccgg atacgccgat agacggcccc
    27781 ttttcgtcca tgatgtctgg ttctttgtac tggatataag acaactccgg tcctatctca
    27841 aagacatgtt tcgccctggc ctcgcaggtc tgcgtccagg caaacatgga caacatgaaa
    27901 gccgccaata caatgcctaa tgcttgtctt gttgttactg ccctttctgt tcttctagtg
    27961 cttcccatca acgcctcctt ttcatttttt tgtattactt tatcctgtat tacttcccgg
    28021 cggcgactat ttaacatttt ttcgtcatga gcgtgaaaaa agcaaacaaa agggggagga
    28081 gggcaagaat aagaaatttc ttttccagaa aaaatacaga agcgacagta gtgttgaggc
    28141 ttggccgaga tcaccgtctc aatccagtag gaacagcatc ggcccgacgg cgccctcgaa
    28201 ccagagaaag aaagcggcta cacctgcaac gaagcagccg ccggcaatcc gccagtttgt
    28261 gaatctgccg gtattgaagg caaacaaggc aaccaggcag gccatgaacg ccagacagta
    28321 tgggcagaaa gtgtcgtgca ctatttggaa acgcacaagg aggacctccc cgcccagggc
    28381 agcggccagc atggaagccc gtaggtgtac gatgtagcgc cgcaactcag gcagggcaat
    28441 caggttgaag aggaacagga caaccatgaa ggatacgccg acgatcttca ggtctatccc
    28501 gaaaagcgag ccgctcaggg tcgcgcaggt ggtttcgcag gagccataat acaccatgat
    28561 tccggccccc agaaggggaa cggcgatatt ggcgatgtcc ctggttgtgt ttaggtgagt
    28621 acgcatcgcg tgttctattc cggcaatcaa agcaaccgtt gcaaggcagc gacgatcgct
    28681 ttgccgcccg tgtagctttc cttgaaaccg ttctccccgg cgataacgca tgtcggcgta
    28741 gccctgaccc gatcttcctt tatcttgata ttgtagaacg aaaagattgt tgaaacacag
    28801 gagttgcgct cttgcgtgcg aacgatgccc gccgtgtcga ggtacgcctc tatgctgcgc
    28861 ccgttttcag ccgcataatt gaaatgggcc ttgagcgtga tggccgtgtc cagattgttg
    28921 tcgtaggcgt gcaggcagcg aagatattct tttgcatatt caatgctttg cttgctcaat
    28981 ggcacatcca cgaacaccac gcgtatcctg ttcagctcta ccagcttcct gaccagcgga
    29041 tcaacgctcg cttttatggc ccggcagggg gggcagaagt agtccgtata taggcacacc
    29101 tctacgggac cgttgccgta aaccggatat gccggctgat ttgccgccgt ccgtggcgat
    29161 gcactgataa caggtgcaga cgtggcaaat agaaagaggg acaccagggt tacgacaagg
    29221 cagctcgctg atgccggatg ccgaattaca acattatata tttgttgcat gcctctctcc
    29281 ttccacgcgg tctgcctttg cggttttctt ctatattact tctgctctca gggttcctgt
    29341 tgccgacggc attgagaggc gaattaccac tgttgccgaa agctgcgcat atgatgtata
    29401 ttgtttacgt ttaattctat acgagcaata atccgtataa tattaatgta ttcaggtata
    29461 gacggcatat ataaattgca taaataaatg agagaaaata taaaatataa acaaagaagc
    29521 aaaaatatta cttgacatat actgtaaagt atggcatgat cgttttcaag aaagggggag
    29581 aacatgccgg aaggaaaaag cctgttgtta gacgaagaag gggttcgcaa gaattgggcg
    29641 gcccacatca acgccaatcc tctcgatcgt ccccggctgg aggccataca cggccaggtg
    29701 tgggacacgg aggagttgtc acgcgattac gacgttcatg gattcatggc gccgctggta
    29761 attgccacct gcaaggccac caagaagcga ggttcgctgc tgtttcagca tcatcctcgg
    29821 ttctattttg gctttgttaa ggtggccacg gaggataacg atgctcaata acgatacatt
    29881 ttatccgacg cctgtgccgc tcattgatcg tatgatcgcc aaaatcaagg gcaggccgca
    29941 aaccgttctg gatccggcgg cgggaatggc ggctattatt gagcgcatgc gtgattattc
    30001 cacgatggac agtgacaata ggtattgcta tcgtccctat gagaattgtt cgtattcggc
    30061 tatcgagatc gatacgaacc tgcaagcgat cctcgacaag aagcctttcg tttccctgat
    30121 cgacaccgat tttctccagt attccggccg tgatcatttc gatctgatca ttgccaatcc
    30181 gccctttgac cggggggacc tgcacctgct caaggcaatc gacatcatgt atcgggggga
    30241 aatcgtatat ctcctgaatg cggaaacgat tcgcaatccc tacacccatt cccgcaagga
    30301 gcttgcgcaa cgccttgcgg agcgtaacgc cgaaatcgaa ttcgtgccgg acgccttcaa
    30361 ggacgcagaa cgacagacgg acgtggaagt ggccctgatc tatatcaggg tcgagcgcac
    30421 tgtggaggag gacctgtttg ccggtgtgga ggacgaggcg caggaatgcg acgctacggc
    30481 tgcaaagcag gactgggaag tggccacgaa gcacaggata cgagacatgg tcgccgacta
    30541 taaccggacc atcgacatag ggacgaaaac gattctcgat tattacaaga actacaacaa
    30601 gatcgggaag tatctcgggc tcaattgcga ggcgaaggag cggctatacg agcgacggga
    30661 tgacctcacc agcctggtgc gcggaaaggt gaatgatttc atcaagtccg tgcgtctcaa
    30721 ttattggcgg caggcgctgg agctgaagga agtccggagc cggatgacca ccgacaaggc
    30781 caaggagttc gagagccaga ttcaaaagcg gcaaaagatg gactttacgg aaagcaatat
    30841 ccggcagttc attgtaaatc tcatcaatgg ctacgagggc atgctcaccg atgccgtaac
    30901 caagcttttc gataagttca caatcgaaca ttgctacagc ggcgggatcg tcgaggaaaa
    30961 catccatatg ttcaatggat ggaagactaa tcaggcattc aaggtgaata aaaaggtcat
    31021 cattcctatc ggctaccggc tctccgacgg cgagggcccg tttacatacg ggacatacgg
    31081 gcatcgttgg aaagcaaact cctggaaaat cagcgagaag acgcgggata tagacattgt
    31141 gatgagctat ttcagcgcgg acacggatta tgtcccgatc gctgacgctg tggccgcagc
    31201 gttggacagg caggaaagca agaacatcga caccacgttc ttcaggatca cctgttacaa
    31261 aaaagggact atgcacatgg tcttcaagga cgacgatgtc ctgagaaggt tcaatatcgt
    31321 ggcctgccgg gccaagggat ggctgcccat ggattacggg caaaaacggt acgaagactg
    31381 cgacgaagaa acccaggctg tagttaagtc gttcgaggga atcccgcaat acaacagcaa
    31441 cgtcaagcgg cccctgttcg ctaccaagcc gcttctggcg attgccgcgt gaccgggaag
    31501 aatcaggctt aacaaacgta ttatatataa taaggaagga ggagtagtgt cgcaaacgat
    31561 agtaattgag gattccaaat tttctcacag cctggcaggc catttcgcca atgtctgttg
    31621 ggtttgcaaa gcggcggcga aagaaccgga tgtgagagcg agcatcaagc aacgcattgc
    31681 gtgccgtgag ggcatcctgt gcgccacgga cggctaccgc ctgcatttgt accggcagga
    31741 tagcgatagc ttacctgata gtgcaatcgt ccccgaaggc gtgtgggcaa tcaaaagcaa
    31801 gacggcaagg gtcatcacgc ttgagcttgc cgaagacgag tttgccgccg attttccgaa
    31861 tttctggatt gtcttccagg accggccgaa agtgcttggc tcgttcagtt cattgggcgc
    31921 ctccggcaaa ggggattacc cgtatgtgtt tggcgagttt ctgtaccggc tcaatacgca
    31981 tgccggcaag gccttcaggg ccgaccatgt agctgatgcc ttcatgccgg atcgccatat
    32041 ccaggtagag gctcacgcca agggcaagat ggccgtattt ggcgacgata cgctgattgc
    32101 ggctctcatg gcaataatca cgacggataa tggaggcaat cgaagatggg catgatgggt
    32161 ggccaaaagt ccgggtgggt tgataccgca cctgagaaaa atgaggcgat cgcccgaatg
    32221 ctgtggccgg atgcatacgc cggcgccggc accaacaaga tgcaggcgcc ggattttacg
    32281 acgccggaag gcaggttgat tcttttgcag ttgatgatgg agcgggacga ttggctcatg
    32341 ttttccatgc tgaagtccgt cgccataaat catcggggaa cgattttttg gatagacaca
    32401 ggctatctgt tcttgccgga caaattgcgc gacgcggccc tgggctggct ggctggctcc
    32461 aatacgggcg gacggagtgt gtagtttgat cgggcgcacc gttgcaataa tcgccttctg
    32521 cctgtctgtt gcggcctttt gctcagtctt ggcgacatac atcagaggcg cattacaggc
    32581 aggaaaagac ggcccggcgc aacaacacgt cgcgtctccg tacatcaaga agcagatgca
    32641 gtatcacggg gtcaatgtcc tttttgagga ttggaagggc aacctctttt ttgagaggca
    32701 ggggcaaaag atcgtcctcg ggcagggccg ggagatcgac aaatgacgaa gatgcaccag
    32761 gtaagaaaag aatttggcga accgtttcgg gatgtcgtca aggggtttgc aatcatggga
    32821 tattcccgca aggccacagc ggagttcgtg gaaataaccg actcctggtt taacgagcta
    32881 tgcgagcggt ttgatctcag aaagcatttc cggcgtcagc ggaaagattt cgtttctgtg
    32941 tgcaaacctc cggggcatcc caaagggaag acgattcggc agcctcaaag atacagcgac
    33001 gagtacttgc tggccgtatt gagaggatat tccaaacgaa taagccaggt aaggttcaat
    33061 cagttgcaag ggggaaggcc ttgcgccgat acgtacctca aacggttcgg gtcatggcgg
    33121 aaagcgaggg agttggcggc aaagtgcgaa gacgagcgcg agaggaatgg agaaaattat
    33181 ggatagcaat aaaatgctca aaacagtttt gcccgaagga tgcaaatttc gcaatctggc
    33241 caagctcggt gaaaaaccgg attgggagat tgtgccgaaa cgcaaaccac ggcggatgtc
    33301 ctatgccgag gtgcttcaag tcatcacgaa atatgctccg gaaatgcagc attgctttga
    33361 gaataaattc ggccggtttc ctaaaggcgg cgaggcggca atgatcgtca actacctgaa
    33421 tgatacggct gatctctatc gtgcggaaag agagaacatt gtaaacgact tggtagatgc
    33481 cgccacggaa atcatcgtga ggtgcgggtg atgcagtcga aagaggattt atatcggaat
    33541 gggcttaaag aggcgatact ggctttgtca tattttagga accgacatgt ccggcggaat
    33601 ctatacgcac actcaaagcg acgcgaacca aagacattct ctgtatataa acagtacaca
    33661 cgcctcgcca gacactacat tcgcaaagca cgcaaaagcg gattcacagg ttcggttgta
    33721 gctgcgataa cggcgcttga cgggcaaggc tgaggcaaaa cagtgataat ttggggcggc
    33781 cgcgaaagca tgattatgcg ccgcttttag gttttatgag atcaaccaaa caataagaaa
    33841 ggagagataa aaaagatggc aagattcaag acgatggttc ttatggcagt tacggcggca
    33901 atgatggcgg gatgcggggc ggcggcgccg caatatctcg attacctggg ccatagaatc
    33961 ccaaccacca agttgtctgc accgcaagac atcgcggcga aaatggcgca agacggggtc
    34021 gtgtccatgg aggcattcgg agcgttgagt gccggcaaag ccgccggcgc ggcaaatgcg
    34081 gctgcgttgc aggtagaggc ctccagcgtt gaaacaatac ggctcgatcc taacgattcc
    34141 gcattgatcg tttccgtccg gaaagacggt gtagatccta atcggatgcc cgacgctttc
    34201 cggaagagtt attttaaggc cttgagccag gtaaacgctg tttccgctgc cctatccttt
    34261 agtaagccgc tggagatttc tcacgtccta aaggggagac atgattattt caccaatcat
    34321 ctcggtacgg aggcctccag gaaaagactc aaggagaagc tggcaatgtg ccaatgggaa
    34381 gggactgcga tccccgtcaa gaaggagggg aatggcgcct tttactatgc cgatatgcag
    34441 aactatgcca tcacgctatc agaattcgcg tttcagatta aatacagcga caaggagaag
    34501 gccttgccgg atacggaccc gtgcagcgcc ggcgaagccg acaaactgct ccggcaactg
    34561 atcgccctga tcgcaggagg agagaaatga agaagatcat cccaaagctc ctcctcctgg
    34621 tggttatggc ggcaatgatg gcaggatgcg gcaaaaatca cgtcctcgtt gccggtcgtt
    34681 cgatacctct ttatgaaatg gagtcggtgg cggacatcga caagaagatg gcggaagacg
    34741 gggctgtatc catgaaggat ttcgcggcag cgagagccgc agaggcaagg cagagagccg
    34801 atctcgccgg cgtggaaagc gccgcgttgt tgaccgtcga tgcggctctt tatgggatgc
    34861 agttcgcgcc tgcggtgttc ggaaccatgt cggcagcaag caacgcagcc atggcgtatc
    34921 tgtctatagg cgacttgata acggcatacg ggcgatacct ggagcggaaa aactcggcct
    34981 acgccggcct caagttcagc aagcctctgg aaatttaccg ggtccgtaac ggcgaaaacg
    35041 tgttcttcgc ggatcttctc ggcacggaag actacagaag gaatctgagg gtgaagatgg
    35101 caacgtgcgg atggcttgag acgatcatac cggacaagaa aaagggcaac ggcgcttact
    35161 atcgcgccgg tacagacaat tcctcaatca cggcgaatga gcttctgttc cagatcaaat
    35221 acggcgataa cgcggcttcc ctgccggaga cgaacccgtg cagcatcaag gaggcggaga
    35281 tgctcctcgt gcagcgagtt gcgctgatta tgggaagtaa agccaaaaag taggggggat
    35341 attcatgaat gtagaaaaag gaagatactg ggacaaacgg tggagcctcg tagattgttg
    35401 cactcgcccc gaccgtctta acattccctt gcggacccgg aaacctacca ttttcgcggt
    35461 tcgcaatgac ctctttcatg aggcggtgcc tgcatcgttc attgatgatg cctatgaggt
    35521 tatggcagcg tgtcctcatc acacgttttt agtcctgacg aagcgggcgc aaaacattgt
    35581 gccgaaactc tatgacatta ccgttgattg cccgattcgt gaactgggcg ggggagacta
    35641 tctgccgaat gtttggcatg gtctcaccat ttgcaaccaa cccgagcttg acgcgaaaat
    35701 gggcgacttc ctgaaggtgc cgggaaagaa gtggatgtcg attgaaccca tgttggggga
    35761 gattgacatt aagccatatc tgtcaaaacc aatgtattat tgctctgttt gcggaaaaga
    35821 gaaattccgt gattactgca aaaaatgcgg aacctacgac aataccgcta caaataaact
    35881 tgcgggcatt gacggtgtgg ttttgggcgg cgagactggc cccggaccgc ggcttatgca
    35941 cccggattgg gtgaggaatg tcaggaatga ttgcgcggcg gcgggagtgc cgttcttctt
    36001 ttcgcagtgg gggagctata ccgggatcag ggctaccggg tacgatttcc acaacgaagt
    36061 actcaatcct gctggacata acaggacaaa gagtaatggc ggacgcctcc tggatggccg
    36121 gacgcacgat gatttggcgt gggggaagaa agaagggata tgaaccgata ttttttcggg
    36181 aacaggggcg ggaaatactt gctcatcaaa gcgccggacc tcgagacggc gatcaaggtt
    36241 tttgctgttg tggaactctt ccccgagcaa tacctgccgg gcgatatggt gtgttccagg
    36301 aagtatgact cggtactgga acggtacgtc atagagccga agccaagggc gacgattccc
    36361 gtgtacatcg aagtcgaaga gacgtttgtg accaagtctt ccgtatcggg aattacacga
    36421 acagaaacct gcaaggtcca gaagaaaatt cacgacctcc tcttggcaga ctgctccgag
    36481 caccacttgc cgccttcctc gatcctcccg gaaaaaaccg gagacgatga aacaaccggc
    36541 cagatcacaa gcgttgtaat caacggccag gctattatcg tgagcggcag caaagccgca
    36601 ttgcggaata gccacgacga gcttcagcgg aaaaagcacg aattggaagt catgcagcgg
    36661 cagtgtcgag cagccgtgga agcgatccag aaggatttga agaaaaagca ggaattgctt
    36721 tacgcggtct gcacctacat ggggcagggg gaagagatcg ttccactact aacggggcag
    36781 aacgctccgg aggacgcgcc tttgtcggtg ttccaagagg tcctctatat ggatgaggag
    36841 atgggggcgt gggacaagga ttctctcgac ggcatcgact tttcccgcat cgaagagttc
    36901 gacgcatgga ttgcccggca ctacgaaaca tttctttacc gtgaaaagag cgtctgcgtc
    36961 ttccagatca ggcgatatag caaggactac ggcgatcctc tggaaaacgc cgtgctgaat
    37021 gcagaaaata tgaagtcgta tttcttggtg cgcaacggcg gtaatctcta tcggatttac
    37081 gggaacgtcc atatacgaga acccgtgttt cccaccaagg acgagtacga aagcatcatt
    37141 gccaaatatc gggacacgaa ttcaagttgg ggagaaaaaa acctgaacaa caggcacaag
    37201 cactacatgt acggactgat ttacctccag ggcctgatcg aacggtcgtc tatctttgga
    37261 aacgggctta aaggcgtcag ccttccaaag ggcattttta cacaagcaca gatagagctg
    37321 atacgcgact cggagcagag ccaatggatt accgacggga agccttcatg gaatgatttc
    37381 cgcaaaaaca accaagccac cataggtata ggctcgcgca tagtcctcat gcctgtccga
    37441 ttcagcccga aagacgattt gtggcgcctc gggaacaccc cggcggcagg ccttcctccc
    37501 tggagtgaaa catatcttgt ggaggccgag ggagatggtt ggggctacat ggccaaaggc
    37561 agttttatca ttcgttacaa ttcggcagac actatccacg atgaatacga gggcttccgt
    37621 ccccggaagc gacgtgtcag ctatcgattc tttccggatg aagtgatcaa cttcgatcgc
    37681 atcaccgtgg aggagtgcga ctactacatc aaaaacaggc tggaacggaa gcactaccgg
    37741 gacatcctgc ctgttcttta ctatgtccgg cgatgcaagg aaagagagcg ggccctggaa
    37801 aacaacttcg tctcttttat ggcggggcgg atgtctctcg atgaaagact gctgccggcc
    37861 ttgcgcgaag tgattgcctg gtggaagctc aagaacaagt ggaagcgggg attgatgacg
    37921 gacgaccaga aggcggtcag aatgatcgag aagaagatgc aggctattgc cgcggaggga
    37981 atatgagggc cggctatttt cggtgtcgct cgaagacacc gcgcaatgaa aatatataaa
    38041 acataaaatg tataatataa tatatcatgt catatttatt attgacaaaa aacgtaaagt
    38101 atggcatgat gcttttcaag aaaggggggg ggcttaaatg cctgatattg atcacaacga
    38161 ctacatggag tttctttgtt gggttgatcg gtgtctgttt ccgcaggatc tcatggaaga
    38221 gcttgtggcg cactgtggtg ccacccacga agaagcaaag gagatagcgt tgcttttctt
    38281 ttcctggaaa ggaacagata gcaaggaagg gaaaaatctt tcgtgggcgt acagcaacta
    38341 ttgcctcgaa cccggtcgct ataaagaaat aataaatagt tacctgaaat ggcagtacgg
    38401 gaagtggagg aagctaagat gccgttagga gttgtggaaa aaactggtta taacccccct
    38461 ggagttccta ctaatgggcg caagccgcat ttgggcgata taatccttaa taaattctat
    38521 catctcattc gtgaagggcg tgaagacctc gagcaatttg aattgattga tcgcttggaa
    38581 aagccggtta tgtatttcgt gttttctggc accaaacgcc aaatactgga ggtgaacaag
    38641 ctactgcaac aacatccata cgctttcgca attaaaacca acctgggcta cgatttatct
    38701 aaattgcccg aaaaatatgc ccacactgca caagtgtata tttctcgttt cccacatctg
    38761 atcaaattta tctcgaaata tgaatcaacg atacccagtg atttgtgggg gattcttttt
    38821 gggtacaagc taaacgaagt ccacctgtac acttacgact gggtagagtg ggccaaaatc
    38881 catttgcctg atagagtaga gggaaaacgc gatactgcca ccagagattt tagggtagaa
    38941 atataaaata taaacgactg tataaggtgc atctttggtg tatggtgtta tagaggctta
    39001 atttaaacgc tcatgataaa tattaagttc tttgtgacgt atttacttga catcatgaaa
    39061 tatttggtgt aaagtgggcc ctcaaagaag ctaaaaacga acaatgcaaa tcctctccgt
    39121 gtagcgcagg ccggtagcgt gcctgaactg ggatcaggaa gtcgctggtt caaatccagc
    39181 cacggagacc ataactatgc gtgacacgcc tggcttgatc gaaaggtttg agttcatagc
    39241 ggctgacggt ggccgggtcg aacgcgccgt ccccccgcat ttgaaggaaa cacttgcgtg
    39301 ggaagtcgat acaacgtgcc cgcagcgatg caagctggcg acatgctgcc atccattggt
    39361 ccaggacaat aaggacaaat atgtctatca caaactctgc acgctatcgg tgatcaaagc
    39421 gggactgatt tcaccgccgg agatcaacgg ttggattgag agcgaagagg agggaaacga
    39481 agaacatgac tgaaggccga cagatgatcg catctatccc tgtccttggt gtgatcaaac
    39541 atcccaggga tgggaagcat ttcatcgtgc cgccggaagt ggcggcggtg tacggcgaga
    39601 atccatgccg tctcgatttc atgctgcccg gcgatgatgt ggaggccgtc ttccaggaag
    39661 cttaccggcg atttgtaggc ggcgggggcc tgcgctgtat cggcagcaac ggcgtcggct
    39721 acgatcggga tttcaagacg ggcaatcgcg ttcagaaggc ttgtccgtgc gagttcctgg
    39781 ataacaacca atgcgctttg accggggcat tccatatact catcccccgt gtgtccctgg
    39841 gaggacagtt tgtcatttac acgaagtcga agcggtcctc ggcgatgatc agaatgacgc
    39901 tgaagaacct ccagggcatg tacggcaagg tgacgggatt atcaatgagc ctgacccgcg
    39961 tgcctgtaaa gatcaagtac gacaagggca agaacaagga atattggatg ctcaacatcg
    40021 tgccgccgac aatggacgtt gtggcgcagg cgatgccggc cgatccgcac agcaccgagg
    40081 cgtggcgtag caggcaagaa gcaggcgaag aaaacatgga acaggcgcag gcggagggcg
    40141 aggcgcagca gatcgtgcca atgacggatg agcaaaggag aaggctcgtg acgctcatgg
    40201 aagccaaagg aatcgggact tcggcagata aagcggcttt ctttgcccac gtcctgggag
    40261 ggcgggagaa atcggagaaa ttcgcccgtg acttcatcgc aaatttcgag gagaaacata
    40321 gggcgtggat ctcgcgcatt atggaggagg cggtggcgag ttgaaagtgt tgagcttcaa
    40381 taattccatt ggattcgaca tgcacggtct atctgcaaaa gcatggtttg acggagcgtg
    40441 cgccggcaat cccggcccaa tgggaatagg cggcctcatc attctggagg gcggtgagaa
    40501 gcatacgatc agcgagcagc gcggctttgg aacaaacaat gttgccgaat ataccgcctt
    40561 gatcgaagtc ctcaagctcg ccattgagct tggcgtcacg gatctgcaag tcaagggcga
    40621 cagccagttg gtcgtgaatc aggtcaacgg cgactgggca gtcaaaaatc gtggccttgt
    40681 tccatactgc gacgcggctg aagtcctcat ggcccgaata ccacgcttca aactgcaatg
    40741 ggtccggagg gagcagaacc aggaagccga ccggttgtct tcatcggcat tgaaaggcgg
    40801 gggaaaaaac ggggaagcgc agggaagccg gcagagaaaa aacctgtccg tcgcggtgcg
    40861 aaaagtagga gatcacatct acgttgcgga gggatcgcac ggcaatcagt acgcggtcga
    40921 tctcaaggca aggacatgct cctgcccgga ctggcaacga aggcaaagtc cgtgcaagca
    40981 catgatggcc gctgaacgcg cacatgaggt gggacatgac agttgaagat atacatcagt
    41041 acggctttga cctgggcgag aaggacggcc aggtagcagc caggtggata cgaaagggga
    41101 tagaggtcga tcacggggac ctgttgtcgg cgacagattc ctacgccgtc ggctaccgca
    41161 aaggcctcaa ctggtctata cgcccgagca tcacggtaat acgcaggaag aagaacaatg
    41221 cctgaaattt ggacaatagg acattccaac catagcgtcg agcacttcct gcacctgttg
    41281 tccatgcacg gaattacggc ggttgcggat gtccggtcca caccctacag cattgccgct
    41341 tcccagttta accgggaggc aatcaaggaa gtgctccaca aagaacgcat ctattacgtg
    41401 cacctcgggg aggaactcgg tccgagaagc ccggaccccg cctgctacga aaacggcaag
    41461 gtcgtttacg gactattggc ggcgacggcg gtattcgccc agggcatcga gcgattaagg
    41521 aagggcatgg agaggtatcg cgtggcgatg atgagcgccg agaaggatcc catcgcctgt
    41581 caccggatgg tcctcatttg ccggcatctc agcatgcacg cggatgacgg gctggcgata
    41641 cggcacattc tggaggacgg cgccatcgag gataacgcgg caagcgagcg gcgactgatg
    41701 caggatctca agatcgccga gttcgacctg ttcaacagca gggaggaaca gatcgccaag
    41761 gcatacgcgc tgcaaagcca gcggatcgca tatgcgttgt aggaacacag gtaggaggcg
    41821 taaaatttga aaaagagcgt gctattgttt cttgaaaaag tttttgccgc aaacctcgac
    41881 ggccgtcttc tccagtcgaa aagcaaggtg gcaaaaaagc tggaggaggc aggctacatt
    41941 gttttcactg aaaaaacctt gggaagagat cgcttcgggt cgattgttgt ggatgggtac
    42001 acgttaacgg tgatgggcaa catggcctat tgcacgagcg atcggtgcaa agaacccgtg
    42061 gaggagcaat aagtgcctcg ccccgcgaaa atgtcggcgc ggagtaaact cacattgtct
    42121 ctggagcgtt tcggtgatat tactttggcg aacatccaat aattaaagga agaaatagat
    42181 gcccatgaac aacaacgatg tcgaggcctt gaaatccaag gcgaaggtag ctgcgaatac
    42241 tctctaccgt atctgccata ccggtcggag atcagcagaa accatggaac tgctcgacat
    42301 tacaagcgag gttcttgcct tgttgaatga tttggtcgat tcattgaatg ctgacgcatc
    42361 ctctgcaacg aatgacgacg catacgtgag ggcaaagatt ttcttgaatg catggtcagg
    42421 tagggtaaat ggctaacaac agcaaagaac ataaaaagaa tccactgcat acgccccgcg
    42481 tcatcgcata cgtgcgcgtg tccacggatg agcaggacgt caacaagcag cgccatgaga
    42541 tcctggaata cgcacaccag aataagatga ccatcgacga attcataatg atcaccgtat
    42601 caagccggaa agcggcgaag gaccggcgga tcgaagaatt gatggaaaag cttgcgccgg
    42661 aggatacgct gatcatatcc gagctgtctc gtctgggccg gtccatcgcg gaggtcatcg
    42721 gcctggtgaa cggcctggtc gagcggaacg tcaggctcat cgccatcaag cagaacatag
    42781 acctggcagg aaagcatgac acgacatcaa aggtgatggt tacggtcttt tctctgctgg
    42841 ccgaactgga gcgcgatctt gtgtctcaaa gaacaaaggc agctttagct gccttgaaag
    42901 cacaagggaa aaagttgggg agaccagtgg gaacaaaagg aaaatcgaaa ctcgatggta
    42961 aggagccgga aatcatagct tttctcgaca agagagtgcc ggtggcggca atagcgcgta
    43021 ttttggggtg ttcgcggcca ggcctcttga atttcatgga aaacaagaag atatacaagg
    43081 caaagcgaag cgaaaaaagg tgattggagt tgtgtgctgt gggacagtca cggcatcacg
    43141 aaaaatctat caatacgtta ttggctcgcc gccgtcttgc agtgatacct aaactggtac
    43201 atgcctaaag caaccaagga gtcttgcaag gacgcatttt tgccttgaca gcatgaaggg
    43261 cagtgtggca tatgcatatc gcttttgagg aacacgattt gaggaaggag ttggaaggag
    43321 tagctttagg agtagcgacc ggcttgagct attaaaaatt atcctatttc cagtaagtta
    43381 taacaacttt ccaaatcctg ttccgcctac catattaaaa aaacggaagc cgcgggcggc
    43441 gacggccgga gtgaatcatc ggcgaagcgg gagcggattg cgaagtagca tttagtattg
    43501 attgaaaaaa acggggtcgt agttaagttg gttataacgc cggcctgtca cgccggaggg
    43561 cgagggttcg agtcccttcg gccccgccaa atatgtcagg ggttggccta tgcggttaac
    43621 ccctttttca tatcaacaga aagatgaagt acgaagggat aatcatccga cctcccagcg
    43681 aggcttacag cctcctgtta caggtcaccg taggttgttc tcataaccgc tgcacctttt
    43741 gtcccactta caaaggcgta aagttccgga tcaaatctcc cgaggaaatc agtgaggata
    43801 tccgtgcggc acggcgttat cgtcaggtgg aaaaggtctt tctctgtgac ggcgatgccc
    43861 tcatcgttcc ccatgcacgg ttgttggata tgctgcacca actgaaagaa agcctgccgg
    43921 ccctcaagag ggtgggactc tacggaaacg ccaagagcat cctccgcaaa tccagggagg
    43981 aacttgggga gctacgggaa acggggttag ggatcgttta cctcggagtg gaaagtggcc
    44041 atcgtgatgt tctccgggac atcaagaagg gggtttccta tgatcagttg gtggaggccg
    44101 gccgccgcgt caaggatgcg gggataagtc tgtccgtgac ggttatcctc ggattggggg
    44161 ggagagatcg gagcgtcgat catgcccggg aaacggcccg tatcctcacg gatatcgatc
    44221 cggattatgt gggggcacta accctgatgc tcgttcccgg cacccccctc catgacgaat
    44281 atgacaccgg aaagttcatc cttcccgaca ctttcgattt tttgaatgaa ttgggcatca
    44341 tgatcgccaa ttccacgttc acgaactgct ttttcacctc caaccatgcc tccaactatc
    44401 tgcccattcg cgcccgtctt ccccaagaga aggaaaagac cgtcgccctc atccaaaagg
    44461 ttatcggtgc agcaaatccc ggactcctgc gccccgaata tgcccgggca ctgtaagttc
    44521 cgaaactggt tacgcacatg aaactcatct cattgataaa aaagatcttc caggagcgtg
    44581 acgcctcacg gctggaggag gaaatccagg ccatcatcga tgccggtgag gaggatgggc
    44641 tgatcgatcc ccaatccgga gagatgatcc aaagcatcct ggaattcaag gatacggtgg
    44701 tccgggaggt gatggtcccc cgaacggaga ttgtcgcggt aagcgccgga gccacgatcg
    44761 aggaaatcgt acggatcatt tctatttatg ggcacacccg catgcctgtc tatcggggtg
    44821 atatcgacca cataatcggg gtggtcaatg tcaaggatct cttgaagttc tggtcccggg
    44881 aggtgacgga aaaggatttc ctctccatcc tgcggaaacc ttactacatc ccggagacca
    44941 aaaacatcag ccaactcctt catgaactga agcagaaaaa gtatcatctg gccatcatca
    45001 tcgacgaata tgggggaacg gccggactgg tgacgctgga ggatctgatg gaggaaatca
    45061 tcggggagat tcatgacgag catgataccc aggaacccgg cattgtggag gaaacggacg
    45121 gttatacctt ggtggacggc cgggtggaaa tcgaggaact cgaggaatat ttcggcgtga
    45181 ccttcgatga aggcaaatat gaaaccctgg gtggattgat cctcaacacc gtcaaacgca
    45241 tccccgctgc gggagagacg ataatcgtcg ataatttcga aatgacgatt gttgccgcgg
    45301 atgaacgccg aataaaaaag gtaaggattc gtaaaatcag gaaggaagat gagcaaaatt
    45361 ctccggaagc ctgaaggcgg caggtcgagt cgtctcgatt cttttgacct cctgctcgcc
    45421 ttgggatcgg gggtgctgct gatcttctcc tttccaaaat tcggcacggg tctcgtggcc
    45481 tggatcagcc tcctgcccct tctttattcc ctgcggggaa agaatgcctc ccatggtttc
    45541 ctgttgggac tggcaaccgg tatcacttcc cagatcggcg tcatgtactg gataacttac
    45601 gtagtcgtca attacggcta cctgccatac ccggtgggca tcgccgccat gctcctttta
    45661 tccggctatt tagggctttt tacgggggta ttcgcggccg cggcggcgta cctgctgggg
    45721 tggcgattgc ctttctgtct caccgctccg gtcctctgga cggtgctgga gttcgggaaa
    45781 tcacatctgc tgacgggctt tccctgggag aatctcggct attcacaata cctgaattta
    45841 ccgattctac agatcgctga tgttgccggc gtctatggag tatcctttct gatcgttttc
    45901 gtcaatgcct tgatatttga ggtggttttc atcaaccggg ggaaagagga aaggaaagaa
    45961 agggtggtaa aaatcatcgc cgtcctgggg attatggtcc tcctgttcgg ttacggcctg
    46021 atgcgcctca aggagatcga tagggcggca tcggcgccgt cgatgacggt caggatcacc
    46081 cagggcaacg tggaccagag tatcaagtgg aacccggcct atcagttgaa aaccattgcg
    46141 gcctatgccg ccctttcacg atccgaagcc gtgaccgaac cggctttgat cgtctggccg
    46201 gaaaccgcct atccggcttt tttccaggat gtcaacgacc tccaccggaa agttgtggga
    46261 ctggtgaagg acaaggcagc ctggtttctc atcggtagtc ccgcctacac gacacagagc
    46321 ggggaaacgg tgctgttcaa cagcgccttc ctgctgactc cggaaggcgc gatcgccggg
    46381 cgctacgaca aggtgcatct ggtgccctac ggggaatatg ttccgttacg gaagttcttt
    46441 cccttcgtca ataaactcgt ggccggcatc ggcgatttcg gcgtcggccc cggttattac
    46501 cccttgacga tgaattcccg gcgcctgggc gtacttatct gttacgaggg gattttccca
    46561 gcggcggccc gagattacaa gcgccgggga gcggatttcc tggtcaatat taccaatgat
    46621 gcgtggttcg gcagtacctc cgccccttat cagcatttgt ccatgacagt gtttagggcc
    46681 gtggaaaatc gcctcgatgt ggtgcgggcc gccaataccg gcatcagcgc ccggatcgac
    46741 gccgccggca ggatagacgg gcggacggaa ctgttccaga ccgcctttca agacattaaa
    46801 gttcaattct acaacaaagg gaccttttat gccgtctggg gcgatttatt cgtctatctg
    46861 tgcatcatcg ccgcggtttt attttttatg atcataccca taaggaggaa gagagatgct
    46921 ggaagggata aatgaaaaga tgacggattt gaaaagaaga atttctcacc tcggagagtg
    46981 tctttgacgt tgccaccaag gaaaacgaaa ttcgggaact ggaaaaaacc gctgcccggg
    47041 aggatttctg gaatgaccag gaaaaggcat ccgcggtctt gcggcagcag acaaaattga
    47101 aggaagcggt aacgcactgg cgaaaccagc agcgacaata tgaagatctg gccatgcttt
    47161 acgacatggc ggcggaagag agcgacgaag cggccctaat ggatgtgggc cgggatatcg
    47221 aggccctgga aaagtccgtc cgggaggaag aattgcgcct tatgctcggg gccccggagg
    47281 accgtctcca tgccatcatg tccatccatg ccggcgctgg tggcacggag gcccaggact
    47341 gggcggaaat gctcctgagg atgtatctgc gctgggccga gagaaagggt tacaaggcca
    47401 ccattatcga ttatctgccc ggtgacgagg cgggcgttaa aagtgtctcc ctcgctatcg
    47461 agggagaata cgcctacgga tatgccaagg cggaggtggg cattcatcgg ctggtgcgca
    47521 tttccccctt cgatgccggg gcccggcggc acacctcctt cgtttccgtc tttgtttatc
    47581 ccgaagtgga cgatagcatt gtcatcgaga ttgacgagaa ggatctccgg atcgacacct
    47641 atcgttcctc cggggccggg ggacagcatg tcaacaagac tgattccgcc gtgcggatca
    47701 cccacctacc aaccggaatc gtcgtccagt gtcagaatga aaggtcccag cacaaaaaca
    47761 aagcgatggc catgaaatat ctgaaatcga gactttttga gatcaagctc caggagcaga
    47821 acgagaagct cgacgaggtg gagaaaacca aaaagggcat cgcctgggga agccagattc
    47881 gttcctatgt cctccatcct tacaagatga tcaaagacca ccgtactaac ctggaggttg
    47941 gcaacgtgac ccggatcttg gatggggata tcgacgattt tatcgaggcc tatctgctag
    48001 caaattagcc tgctcaaggg ctacgaccgg tacagccgat gaagatgaca gcgtgacggt
    48061 ctcggcgggg aaataaattt tcgtgctgcc ggttatttgt ggtataggat acgatagtgc
    48121 ccgtcaggaa acgaaaggag tcagataaaa ccatgtgcgg acataagaga atgaaactgc
    48181 cattaaatat attggctttc ttgatgatcc ttcccgtttt ttcggcccca gctctctggg
    48241 gaggagagat gagaacggcg atttcctccc cgccggccca aacggggtct aagggaggca
    48301 acgaccctgt tccggtggca gcgagatccg tcctgccgct gtccggagag aaagggaatg
    48361 taccgtctga ggtgaaaagt ccggaaaaca gggcaaaggg caaggaggac caacaggaac
    48421 aggatatcat ggaggccgct ctccgactcc tttccgaatc ccagtcctat tgggtaaaag
    48481 gagatgtgga aaacgccatc gaaatgctcg atcaggcgta cgccctgatc ctggaaacaa
    48541 atggcgatcc agaaatcggc cgacagaaag acgatctaag gttgcttatt tccaagagaa
    48601 ttctggcgat ctattccgcc atgcaatccg ccgccaaggg aaagcgcggg gagatccccc
    48661 tcatcatgaa tgccgatgtg gaaaaggaga tccgctcctt tcagaccgtg gagcgggatt
    48721 tcttcatttc atcatatcaa cgttccagtc tttatcgacc ctatatggtt aaggaactga
    48781 aaaggctggg tcttcccgaa gagctttcat ggttgcctct cgtagaaagc ggctataaaa
    48841 tcaaggcatt atcaacggcc agggcattag gactgtggca gttcattccc tccacgggat
    48901 ataagtatgg attggaaaga gatcagtggg tggatgaacg aatggatccg gaaaaatcca
    48961 cccgggcggc catcgcctac ctgagcgatc tccacggcat gttcggagac tggcttaccg
    49021 tcctggcggc atataactgc ggcgaaggcc gggtcatgag agttatctcc cggcaacaca
    49081 tcaattatct ggatcggttc tgggatctgt ataatcaatt gccttatgaa acggcccgct
    49141 atgtgccccg ttttctggcg accatccaca tagtccgcaa ccccgcaaaa tatggcatgg
    49201 atttaggcgt gcccatggag gctcagggca agggccgggg atatgaaacg gtgaagacga
    49261 acaagtcgat gcgcctgaag gatattgccc aacatctcaa tgtgccggag gaggcattgt
    49321 gtcaactcaa tccggaattg cgtcatatga ttaccccgga gaaggaatat ctccttaagg
    49381 ttcctcccga aacggtggaa aaatttgcca gtgtcgccga ttccatcccc cattgggaac
    49441 agccgccgcc gactcccagg acgcgaccgg tggtgatcgt tcatcgagta aagaggggag
    49501 aaaccctggc ttcgattgcc aagaggtatc gcaccacaac caaggcgatc ataagatgca
    49561 acaacctccg ggcaaagcgg ggaataaccg tggggcagca tctctccatc cctattcggg
    49621 aggttaaagc cacaaaaaaa tctacttatc atgttgcggc cggaacggga aagaaagcgg
    49681 gaggcggaga aaaggtcatc cgttaccagg taaagaaagg cgacaccctg tcctcggtgg
    49741 cagccaggaa taataccacc atcggttcta tcaagaaggc caacggcttg aaaagcaatg
    49801 tcctcagaac cggtcaggtg attaggatcg tcaagaccga tgcggccggc ggacccgata
    49861 ccgggaagaa gggcaaacgg ggcgctggaa caaccaaaac gggaaaatca cgttccgcgg
    49921 cccaggttaa ggagaaaacc tacaccgtcc gtaaagggga cagtctgttc cggatcgcca
    49981 ggagtcatga catcgaaatc gaccgtcttt gcgaactgaa caatatgagc ttaaaagagg
    50041 ctcagttgcg ggcagggcaa gtattggtcg tcgaatgaat ggtacctccc ggcggcggag
    50101 atggtagcgt catccaggcc cttagatcat ctttgccaac gtctgattta aaaccccctc
    50161 caccagggcg cgaatttccg cgaggcgcgc ggtcgaatca gcttcgaaac gcagcaccaa
    50221 tacgggctgc gtattggagg gccgcaatag cccccagccg tcggcaaagg gtatcctcac
    50281 cccgtcgata tctatgatct cgtaatcctt ccggaaatgt tccttgatcg cttccactgc
    50341 cttaaatttt acttcgtccg ggcaatcgat gcggatctcc ggggtactga aggtcttggg
    50401 gacatcggcg atgaggtcgc cgatcttttt tcccggggat tgagagagga tttccaagag
    50461 acgaatggcg gcatagatgg cgtcgtcata accgaaataa cgatccgcga agaagagatg
    50521 tccgctcatt tccccggcga gcagggcatg ctccgttttc attttacctt tgatcaggga
    50581 atgcccggcc ttccacatga tgggccggcc gccgtgggcg gcgatatcgt cgtatagctt
    50641 ctgcgagcat tttacctctc ctataatggc ggcgccgggt ttttcccgga ggagaaaccg
    50701 ggaaaagagg aggagcagtt catctcccca gaggacatcc ccccggtcgg tgatcacacc
    50761 gatgcgatcg gcgtcgccgt cgaaggctat cccgacatcg gccccctttt ccttcaccgt
    50821 ggcgatcagc tcccgcaggt tatccggaac ggtgggatcg ggatggtgat ttggaaagcg
    50881 tccgtcgggt tcgcaataga tctccgtgat gatgcaaccc aggcggcgga aaatgggcag
    50941 ggaaaagaga ccgcccgtgc cgttgccggc atcgacgacg atcttcaagc cgggttggat
    51001 tttgacattg ccgacgagat aatcttggta gtccgaagtg atatcccggg tcgtgacggt
    51061 gccgccgcca cgacggccgt aactttccgc ttccatgatc cggcgcagtt cctggatttc
    51121 gtcgccgtaa atggtatctg gaccgataca tatcttgaag ccgttgaaat caggcgggtt
    51181 atgacttccc gttaccatga cgcccccatg agtctcatag tgccggatgg aatagtagag
    51241 catgggggtg gcgcacattc cgagatcgat caccgctatc ccggcgtcgg agagccctcg
    51301 agcgatgatt tgagaaaaac cttccgaact gaggcggcag tctcgcccca gggtcataat
    51361 gccgacgtta tgccgccggc cgtaagcacc gatggcaagt ccaaggtgat agacgaaatc
    51421 ctcgctgaga tcccggccca cgaggccgcg gacatcgtat tcccgaaata cctctgcatt
    51481 catacccaac ctcccaagca tgtatttcta gcaatatata gacataatgt caataatatt
    51541 ttattaagta tcatcatacg gaaccagagg aaaggaaatc ggccaggtcg gcggcggagg
    51601 cgaggatcac ttccgcgccc gcctctttca gctctcgggc ggtgtcgtgc cccgtaagca
    51661 cgccgatggc caggacgccc acatctctgg ccagatgcat atccatcttg tgatctccta
    51721 ccatcgccgc cgtagcggcc ggggcggcca aagtcttcag ggcctggtga aggtgtccgg
    51781 gatggggttt gacatggggg gtgtcatttc gagtcagcac tacatctaca aatacatcga
    51841 tatcggggaa caccgcttcg acggcctctc ggcagtttct ggtcaccacc cctgtatgcc
    51901 gtccctgagc ccgaagcagg gagagcatct ctctgattcc gtaaaaaagg actcccgttt
    51961 gtgccgctgc cacttccata tccctgacca gagtcagtgc cccgcgttca taattcgttt
    52021 cccgcccggg catagaactg gcgatcagtt tttttccggc agcgatcatt tccaggatgt
    52081 acagcttccg cagtccgtca gggctgatgc cgaaactttg cagatggtcc attacccggc
    52141 ggcgcatctc ggcgaaatca atggtcaagc cggcgacagt gccgtcaaaa tcgaaaacaa
    52201 ttgttttcaa actacggatt gaattcatga tttacgcttt ttatcgcttt ccggtaagat
    52261 agtcggcatg taaaaccttg ctgcggatcc ttttatcagt aaggtccggc aattggtggt
    52321 tgaagacaat cttcagaaaa ttcatgatga tgtagaaagt ggcgccccag agaatttctt
    52381 cccgtccgtc ttcgccatga atcagacagg ggaattccca aggccccgga tcggcggtgg
    52441 ccacccggtc cgaagcctta atcagatagc ggccgtagga ggacggagcg aagaaagact
    52501 ccagaggaat ttcgatgaac ttttccacct cgcgattggg actgggtgtc caatggggat
    52561 cggtaagagc gacaaggggg aaaatcgtcc ggcggaagag atggagggaa taggtgggca
    52621 ataccccgag aagcgaaaca cggaacggat ggagatggag ttcctcccag gattcccgga
    52681 gggcgttggc aaaaaacatg gaaacgatcc ttctctcccg actgcatgtg cccatccctc
    52741 cggtttccac agaaaggaga ggtatgggca ggatgccgct acggacaaag atgagactga
    52801 agccccagtc gatgaggtgc tggagcatcc cgcccgggca acttatatcg cctccctggg
    52861 cggtttgaga tgatcttttg atcagttgca atacccaggg attgtcaccc ttttgatgtt
    52921 cggggtggcc atggggacga cgatggagga ggagaaggac gccggctgat aattgacgac
    52981 cgtgacggca actattttcc tcttttattt cattccattt aggcagaaaa tcgttttggt
    53041 ttcgaaaaag taaggatctt accagcaggg gcagattgcc gtcggcggcg gaaatatcgt
    53101 gcttcctaat catgaccgct caattatgtc ataaatcttc cgctcgtacc agcaggaaaa
    53161 accgggcata tcgatgaatc cgcagtggcc tccataaggt tggaccgcga catgcagttt
    53221 ttcctgggtc ggcagggcat gaaaatcttc tatggggata accgggtcat cccgggaaat
    53281 gatgatcgtc gtgggcacgg acaaaccgat aaaagaggcg ccgtcgaggg tgtattgacg
    53341 gaaatagtca agataatctc tgtactcccg gtagtaaacc atgatcgcct ccgtcagttc
    53401 catacaggat cgagtcttca tcatggggcg gaaatcatag agatggggga acagggcctg
    53461 ctttttgttg agtgaacgtt tccatttgtt taagaaataa cggcggtaaa cgccccatcc
    53521 tgcatcgatg gccacggtgg ccttgtgggg gtccaaggcg gggctgacgg caaaaacatg
    53581 actcaggggg ggaagctcct cctcccggct gtgcttcagg gcggcccgga gagcgaagtt
    53641 tcctcccaag gaaaaaccga tcaggtagag acggccgaac gttggcaacc gggcgatatt
    53701 ccgcagggcc tggaaggttt ccctgatcag ggcgccgtgg aagagcccct cgttgagatg
    53761 atgggaatca ccgtggtcgc gaaggttgag acgaaaaata tcgaaaccca ggcggaataa
    53821 ataaccggcg gtggccagga tgtaggtgga atccgcgctg ccctcccagc cgtgaatcag
    53881 ggccacgaga ccccgagagt ttccttccgg atggcgggca tggaggccca ggagacggac
    53941 tccctccccg caagggagga ttatcggtgt cgaggaccgg tggagttctt ccgtgtcctt
    54001 aaggcggtac ttgctccggc ccagaaaggt ctggaggtga gggttacgga aaaagagtcc
    54061 cggtgcaaag atgttgttca tcgtcgggtg ataaaaaagc ccggcctaca gccgagctcg
    54121 ttttggttta cttggtggcg gtgcagggat ttgaaccccg gacactgcgg atatgagccg
    54181 catgctctga ccgactgagc tacaccgccc tggtgatcat tccggataga ggctgctcct
    54241 ataacagtta ccctttcttg tcaaccctta ttgcgcgtgt atattataag cggcgatgac
    54301 gctctcccgg cccaccaggg aaccggcgga gatcacgagc accatgacga tttcatcttt
    54361 gccgtcatta tcgatgtcct taatctggta atccgccacg tagccggtca tcttccgggt
    54421 gtgccagttt tccaccagtc ccatggagtc ccattcgaga ttgtagaatt cgctggccgt
    54481 aaagatcttc acatttttca ggatccggga cgtggtcagg ttcttgacga cgatcaattc
    54541 ccgcttgccg tccttgttgg tgtcgtaggg gatgattcgg gcattcaaat aggtattgta
    54601 gaaggtgtag tcattccccg gcatgtcttc tccgtaatgt tcgatataca tgttgcttcc
    54661 gccgaatacc tcttcgctct tgtagatgaa ctccttgctt cccaacatgg actgaacggc
    54721 ataaagaggt ttgtcagttt catgatatac gcacagatag tcataattat tgaaggagat
    54781 tattttttca gcaccttcgc cgagattgtc cagggtaagt ccgtaaatgt tcaggccctg
    54841 gggaattttg agccgtgcac cttcctgata tttaccattg cgccacacca tttcgttgat
    54901 atcggtctcg aagggacggg aaacaccgat cttctgaccg agaaggcgga tgcccgccgg
    54961 cttggtgtcg atcaccctga acatccaggg caggttatcg gcgatggtga cgaatttgcc
    55021 gtccttccac tccaggacaa aggaggagat ggtgttggtg acgttattcg cctctctctt
    55081 cgtgagcacg ttggagacga tgatctcctt tatgccgttg ccgttgaggt cgatgagatc
    55141 gacccccatg taattgttat agcgtccacc gctaatcttg gcgagcagta gggcgtcatt
    55201 gccttttttc tggtagatat acacgctgtt ttcgtcgata accaccattt cgttccgacc
    55261 gtcgccattg acgtcgccca catcgatccc cttgtagttg gtgggatact tctggctcat
    55321 ccagaagccc cgcttatccc ggggctgggc gccgctgata aaatccggat tgatggcgcc
    55381 ggtaaaggtt gccttcttcc cggtccgcat cccggcgatg atctgcccat ccctttcttt
    55441 ttggaccggc gacgccccgg gagcggtaat ttggggcagg gtggcggcgg tcgtcgcggc
    55501 gggcggaatc ggaccgccaa aagcgacggc aggagcggtt ccgaggatgt gttgctcgat
    55561 ccgcagggca aagtcattga tcttgacaat aacgtcatcc atgccctgag attgggtcga
    55621 gactgtcacg gcgggcttat cggcggcaat gtcgaccagc ctgccgtcaa tgcttacact
    55681 gttgccgatt ttcgtgatcg tgcccgccac cacgtagttc accgccatct ttttccccag
    55741 gccgtttatg tcggcaaggg tcatttcttt taactttacc gtctttacga catcggtgat
    55801 tttgtctttg ctcaccactt caatcttgtc gctggcggcg atgcgggaag cgagcatgac
    55861 ctggatacct tgctgcacat agtcgatatt gtccgcgctg tttacggcaa atggcaatac
    55921 ggccacgctt tttttgtcct ttgcccccag gggcgccgac aggatgataa ccaggcagat
    55981 tccggccatt gtcaggaaat aaaatctatt gtactgcttc atatacttaa atcctttgct
    56041 tggtatggcc ggcggagtcg aggcttagtc tcgttattcg tacgatgatt cggtacatca
    56101 tgatcggtat gaaaacgaat cttaatgccc atggatgttt ctgccgccgg agcattagta
    56161 agttccatcc ccgcagtgca aggatgagcc gcttatttta ctcttctcag taacatgccc
    56221 cccggctaaa ttcaagacgc tttttacctt cccatttttt cgatggggga agcgcggggt
    56281 tgcctaacaa cggatagcga tgaacattca tttcatgtat gtgacgatga ggcccaaata
    56341 ttcatggatg gccgcttccg ccttctgcag gggaacggtg ccggggaaaa aactgccggg
    56401 acggaaatgc gtctgaggga cggccagata attggtcggc gccggaatag gggcaaggcc
    56461 ctgcttgcga aagagggcca tggaccgggg catatgaata gccgaggtca ccaggataaa
    56521 aggactcttg ccgatcacgg cggcgatcag acgggcctga tcggaggtgt ccagggattg
    56581 atcttcccgg atcaggtctt cttcccgcgc gcccatcata agggcgacat tggccagcgt
    56641 ggcggcctcc ggctgaacat cctgaaagac cgccccgccc gacaggagca gtttggttcc
    56701 ggggaggcgg tgatagatgc gcatgccctc aagaaggcgg gccagggact cgggtgtaag
    56761 ccggctggaa agggggagtt ctccctcagg aaagctgccg cctcctaaaa ccaccacata
    56821 tctgatgtcc cgcgcctggg gatggttttc cgggttcagg agcggcggat aacggttctc
    56881 caggggggca acaagagcat tggcgatgaa tccgtagctg aaggcggcca ggaggatcgt
    56941 ggccaggaca atgagaaata taccggtttt ttgccgtttt ttctttctcc cgagcatgag
    57001 aagcccggtg gtaataaatg ctatgatgac cgacaggggc attaaaaaat tactgacgat
    57061 tttttttaaa aggaaaagga tgtccattag ttgaactcct gaccggtgtg ccggtaatca
    57121 tccggatcgg taagatccgg aaaggggatg gtgagatgat aaatgccgtg ttccatcacg
    57181 gcctgcatgg gcagtccccc cttttcaaac accttgataa tggccttgtt atccgccaaa
    57241 acatcggctg taaccccccg gagtcctttt tttctcgcca ccgtcatgat catctggagc
    57301 aacgccgtgg ctatcccatt tccctgatat gattcttcca caacgaaggc ggtatcgccg
    57361 tagggacatt cctcctggcg tacatatctc ccttcggcga cgattctggg catccccccc
    57421 tcttccacga caccgacgat ggacatgatc cggcgataat cgatgttgac gtattcctgc
    57481 atcttttcat ggggcataac cttgatttca ctgaagtagc ggtaatagat ggtacggtcg
    57541 gaaaactggt aaaaaaggcg gcgcatctcc tcttcgtccg acggcttgat ggcccggaaa
    57601 tggattttcc ttccgtcctt caaaggataa tccgacacga tatcgaaagg gtacatggcg
    57661 cccgactcgg atagatatat ctggtcggga aagaggatgt ggtgcgcttt cgcctgccgg
    57721 atcagttccg cgcggtcaga cgggtgagcg atgtcgatga gggcctgtgc ccgttcccgg
    57781 agtgtccgac cggataggga ggcgataccg tattcagtgg cgacgagatc gaggacttcg
    57841 cggttactga aaagattggg atatctaacc gcggagacca ggatgttgct ttcaccgcag
    57901 aggttgcggc tgggcagggc gaagacagcg cgaccgcccc gggaatgtaa ggagccggca
    57961 aaaaattctt ccgcctgacc cggcccgcca ccgacgttgc cctggccggt atggagggcg
    58021 acaatgccgg tcaggtctat cttgcgggcc gggagaatgg cgagcatgcc gtcgttaagg
    58081 ctgatgcgga agtggtcgga gacgatgtcg atgccctgga attccacccg gggattgtcg
    58141 tggagccact tcatcagctc cggggtcccc aaggcgtagg atgtgagaga cttgttgcgg
    58201 aaatagccct tgtaacggtt ggttacggcg ccgcagttga ccaggtccat gagggcgtcg
    58261 gtaaagaaaa aggtgtgaat acccaaatcc cgttttttgg taagctttcg accgagagac
    58321 tcgaagaggg tgcccggata aaaggagagg caggcaccgt cggggatcac cgtggctacc
    58381 tggtctgcta cccgctccat gacggggtcc acggcggggc ggggcagata aatcggcgtt
    58441 tccgaggcac ggaccaggta attgaactcg tcggcatgga cgaaggtgtt tcccattgtt
    58501 ctggggacgg cggggttgat ctctcctacc accagcgacg ccttttccat ggcatatctg
    58561 gcggcatcca ctgccactcc cagactggcg aaatcggcgt cgtcgaaggg agagatctgg
    58621 ataaaggcca catcgacccg gatcgcaccg gaggcaataa gagcgggaat gcgggagatc
    58681 cggctgggaa tcagatccac cgttccggag gtgatggccg cttcggccag ccatccggaa
    58741 tagaaggttt tcaggcggaa tttgctacgt ccggcttggg gctgaaaggc aatagcctcg
    58801 ccgatgctgg ccagttgtat cagttccaga tcgttgatat tgtttccaga agcggcaagg
    58861 agggccttgg aaagcgtccg gggttccgcc gctccagttc ccaaaaagat gctcatgcct
    58921 gggcgcagga gagccaggac ctcggaggga gtgacgatct tggaatggat ctctttgttc
    58981 atgaccctat gggacacctt ttcattattg ataagacggt attgcgagag cggcttattt
    59041 gtcaagtcaa aaggaacagc ttgacggcgg gtccgggagt gtattagtaa agccgcatcg
    59101 atcgttagaa attctttcag gagaaccaac catgattccc cgttattccc gagaaaagat
    59161 ggcgatgatc tggagccccg agcatcgcta tcagaagtgg ctctctatcg agattctcgc
    59221 ctgtgaggct atgagcaaac tgggactcgt tcccgaatcg tccctgacga tcataaagga
    59281 aaaggccggg tttgatgtga agcgtatcga cgagatcgaa atgaccacga aacacgacgt
    59341 catcgccttc ctcacgtccg tgacggaaaa ggtgggtccc gacggacgat tccttcacat
    59401 ggggctcaca tcatcggaca tacttgatac ctccctggcc tgcctgctcc aggaggccgc
    59461 cgacatcctc ctggccgatc tggatgaact gctcaaggtc ctcaaacggc gcgcccatga
    59521 ccacaaggat accatcatga tcggccgttc ccatggtatc cacgccgaac cgattacctt
    59581 cggactcaag ctcgccaact ggtacgggga gatgcagcga aaccgggagc gcctcaagag
    59641 ggcgcgcgcg gcggttagcg tgggcaagat cgccggggcc gtagggacgt tttccttcgt
    59701 cgatcccttc gtcgaggaat atgtatgcgt ccggctcggc ctccaacccg cccctgtgtc
    59761 ttcccagatc gtccagcggg accgtcacgc cgaatacttt accgtcctgg ccatcatcgc
    59821 ttcctccctg gacaagttcg cccaggagat ccggctcctc cagcgtacgg aagtccggga
    59881 ggtggaggaa tatttttcac ccggtcagaa aggttcgtcc gccatgcctc acaaacgcaa
    59941 ccccgttcta tcggaaaacc tttcgggact ggcgagactc atgcgctcct atgcactggc
    60001 ggcgctggaa aacgtgccgt tgtggcacga acgggacatc agtcattctt ccgtcgaacg
    60061 ggtgatagct cccgacgcca cgatccttct ggatttcatg ttgggccggt ttaccaggat
    60121 ggtaaaaaaa ttggtgatct atccggagcg gatgcaggcc aatatccaga tgacccatgg
    60181 cgtcatttac tcccagatgg ttcttctcga actgatcgcc aaaggcatga ccagggaaga
    60241 cgcatacgcc ctcgtgcagc gccaggcgat gcgggcctgg gaggagggga aggatttcct
    60301 gaccctcttg aaaaaagaca aaaaggtacg ggagttcctt gacgacgtgc aactggcgga
    60361 actcttccgg acggaaaatt ttctgggcca ggtggattta attttccgcc gggtcttcga
    60421 aaccgaagcg gcgggcggag ggtttatgtc ttcaaggacg gaaacatgat cctgatgggc
    60481 gcagatatat ttaccgctgt ttgggacgta gatgatagtt gtcgggccat ctgcaactgg
    60541 atgatcgggg agggacgagc gtgaaaaagg tgatgccact gtggatatct atgctgatcc
    60601 tgaccttcac gggttgtggc tacctccagg gttatctcca gatgatcgaa gaacagggtg
    60661 tatccgagga ataccaacga attctcaggg aatggacacg ggagatgacg gcccactccc
    60721 agttcgagac gaaattctac atcagcgcta cctatcgctc cttggccttc aacgaggctt
    60781 tcctccggga acaggcccgg gtccgacagt ggtcggaagc acgggtaaag gaacgggaag
    60841 aggcctggat gcagaccgat tccgcatacc gggaattcct cgtatacgct tacaccgaca
    60901 acatggaggc caacgatctc gatcggcagg gttccctgtg gagtgttttt ttgagagaag
    60961 gcacggggaa acgttacgaa cctctggaga tcaggagggt ggaacggatc accccggaga
    61021 tcgaagcatt tttccccttt gtcaataaat attacggtcg gtgttatatc gtgaagtttc
    61081 ccgccggaga gacgaatatc gaccagcacc tggtcttttc cagcgtcctg gggaaggtcg
    61141 atctggtatg gcaggcggga ggtcatactc ccattgcccc gagtcgttag gttcagggaa
    61201 actatgactt aacgatgacc gtgtcccgga gccattggac atcatcccgg tctggataat
    61261 cgatgttgta atgcaacccg cggctttcct tacgcatccg ggcacagcgg atgatgattg
    61321 ccgccaccgt ggcgatattg cgcagttcca gaaggtcccg ggtcaccacg aaatttcggt
    61381 agtaatcgtc tatttcccag gagatcagtt cgacgcgccg atgtgccctt tccaggcgct
    61441 tgtcggaccg tacaatgcct acatagtgcc acatgcagcg tcggatttca tcccagttat
    61501 gggaaacgac aatggattcg tcgctttccg tcgcttcccg gggatcccag gggggaatgg
    61561 caatgtcttc atccttgaca cgcgggaacg ctgactgcaa cctcacgaag gcgcggtggg
    61621 caaatacgac cgcctccagc agggaattgc tagccaggcg gttggccccg tgcagaccgg
    61681 tgcaggatac ctccccgaag gcgtacagac ggtcgatgct cgtttcacca tggtggttga
    61741 cttggacgcc gccgcagaga tagtgggccg ccggcaccac cgggatggga tccactgcca
    61801 tatcgatgcc gaagcgaagg cattgctcgt agatattggg gaaacgtctc cggatgaagt
    61861 cacggtccct gtgggtgatg tccaaaagga cgtagtcatc ccccgatttc ttcaattcgg
    61921 tgtcgatggc cttggcgacc acatcccggg gagcgagact attcatggga tgatactttt
    61981 ccatgaaagg ttctccgttt ttgaggcgca gtattgcccc ttcgccccgg accgcctcgc
    62041 tgatcagaaa ggatttggca tccgggtgat agagacaggt ggggtgaaac tggatgaatt
    62101 ccatgttggc gatccgggcg ccggcccggt aggccatggc gatgccgtca cccgtggcga
    62161 tatcggggtt ggtcgtgacc agataaacct tgccgcagcc gccggtggcg aggatgatat
    62221 acttggactt aaaagtatgg atgcgattgt tatgaaaatc caacacatag gcgccaaggc
    62281 agcgattgtg ggcggggtct ttgcggccgg tcaggaacga ttccatgatc agatcgatgg
    62341 cgataaaatt ttcgtagata tggactgatt tctttctgct cacgtgctcg gtgagggccc
    62401 tttcgatctc ccgtcccgtg agatccttgg catgaagaac ccgccgccgg gaatggccgc
    62461 cttcccggcc gaggtcatag cgacaacccc cactgtcctc acgacgggtg aactgcaccc
    62521 cccagtggac cagctcttcg atgcgcgccg gaccctcctc gaccacaaag cggaccacgt
    62581 cttcctggca gagatcagcg ccgcaggcga gggtatcctg gatatgggag gcgaaactgt
    62641 cctcttcgtc ctgaacggcg gcaattccgc cctgagcgta attggtgctg gattcaaatg
    62701 tttccttttt agtgacgatg gccaccgtgc caagctccgc tgctttaatc gcaaaactca
    62761 gtccggcgat cccgctgccc aggacaagaa aatctgtttg tatttccatt tttcggcctt
    62821 gcctcgaaag atatttacaa cttcgcgaaa tttctatata gatttttgcc gcatttgtaa
    62881 aaggaaatca gcgaagccga ttgatattta tggggaggat aaatgctggt tttgggaatc
    62941 gaatcatcct gcgacgagac ggcggccgcc gtggtccggg acggtaaacg ggtactttcc
    63001 aacgtaattg cctcacaggt gaggattcac ggcaaatacg gcggagtggt gccggaaatc
    63061 gcctcgcgta aacacacgga ggccatcgtg tcggtgatcc tccaggcgct ctgtgaagcc
    63121 ggcgccgagc tgaatgacat cgaggggatc gcggtgaccc ggggaccggg actcatcggt
    63181 tcactcctcg ttggtctttc caccgccaag gccctggcct ttgcccggga cctacccctg
    63241 gtgggggtca atcatctcag cggccacata gccgcggcga tgctcatgga tccccctccc
    63301 gcactgccct ttgtcgctct ggtcgtttcg ggcggacaca ccaatatcta tctcgtccgg
    63361 gatttcgacg acattcaaac cttgggacag acccgtgacg acgccgccgg cgaggcattc
    63421 gacaaagcag caaaattatt ggacataggt tatcccggtg gcgtcgttat cgaccagttg
    63481 gcaaaacaag gcgatcccgg cgcggtactg tttccccggg ccatgcggga cagcctggac
    63541 ttcagcttca gcggggtcaa gacctcactg cttaacttgg tgaagaaaca ggggcggcct
    63601 ttctccgccg cagagattcc cgatgtcgtc gccggttttc aagaggcgat cgtagatgtc
    63661 ctcgtccgga agaccttgaa ggcggcgaat caggaggcga tacgtcgggt ggtggtctgc
    63721 ggcggcgtgg cggccaacag ccacctgcgg tcccgttttg agcatgacgc gggagatcag
    63781 gaaatagagg tattcatacc gcccccagtg ttttgtaccg ataatgccgc catgatcgcc
    63841 gtggtcggcg aacggctttt gagtacaggc cgccgggacg gcccagattt gaatgccgtt
    63901 tcacgctggc cgctggaatt acgtctatga atccgatcaa aaggcgtctc cgggatttcc
    63961 atgaacgctt catcaacctc cggggcgaac cgggacatat cgccatggga atggccatcg
    64021 gcgtctttat tggcgtcact cccaccattc cctttcatac cgtcctgatc atcgctgtcg
    64081 gcgtcctttt caaacagaat atcatggcgg ggtatctggg ctcctggctc atctccaatc
    64141 ccctgaccat tccgttattc tacttttacc aatatgaact gggccgtatt cttctgggca
    64201 tccagacggg cgcctgggtg atgccggaac ttaccgtggc ggggatcatg aatatcggct
    64261 ggcacatcct cattcccctg ctggtcggag gagtagtatc ggcgcctttt tttgccattc
    64321 ccgcctattt ttttgcccgc cgcctcgcgg tggcgggcag gcaccgggag gcggcttgat
    64381 tcccgcgggc aagatcctga agaaacatgc cgtgcgaccc cgccggcgtt tcagtcagtc
    64441 cttcctaaag gacgacaatg tggctcggaa aatcgttgga gcggcggacc tgcgggggca
    64501 agacgtggtg ctggaaatcg gctccggtca cggcatcctg acccggctca tggcgccgcg
    64561 ggtaaagaat ctcctggccg tggaaatcga tccgcaattg gttgccgtcc tggaagaaga
    64621 aaccgggagc agggacaatg tcctcatcct ggaagcagat attctcaagc tcgatctggc
    64681 cactcttttg gcggggcatg gaggagaaag gaaaaaaatt gtcggcaata taccatacgg
    64741 aatatctacg gaaatactct ttcgcctcct gacgcaccgg cgcctcatcg cctccgcggt
    64801 gcttatggtc caaaaggaac tggccgaacg actcacggcc gctccgggga cgaaggctta
    64861 cggcatccct accgtgttac tcaccatgta tgcccggatc ggacacttgt tcgatgtccc
    64921 ccctccatgc tttcatcccc gtccccgggt cgtgtcctcg gtcatttctt tgaccttccg
    64981 ggaacggccc ctggtggagt tgtccgatga aaatcatttt tttcggctgg tcaaagcggc
    65041 ctttgcccag cgccgtaaaa caattgccaa caatctccgt gccgccccgg aactcggttg
    65101 taagcaggag gatctggagg aaatgttgga gaagtgcggt ctgaatggac ggatccgagc
    65161 cgagatgctt accgtggaaa ccctgggacg tctgagcaac gaattaacga cggcgcgacg
    65221 aggcgctcca tcgtccggag aggaaggaaa gggcgaaggc cgccaaagtg aagaaggcgc
    65281 cgataaagaa agcgccctgg aaggaaccgg taacggcgga catggtatcg tcaccgatat
    65341 gctcctggag ttcccggatc gataaccccg gcggcaggga ctgggaaaaa gaaccttcga
    65401 acagacagac cccgaaaacc attccgacgt tcatgacggt gttgtaaata gccgctgcgg
    65461 ccccctggct ttccggcggc gccagggaca tgacgagatt gttgttggcc gggaagaaaa
    65521 aggcataggc cacggccagc aggaccaggt aaatcaccgc cggccacagt cccggttcgg
    65581 gaatggtaaa agcaaagagc atacaggcag cgaaggccag cgccatggcg accgtgcaaa
    65641 gacggcgggg atcaatacgg tcggaaagct tacccgcata agggccgatg ggcatgtaaa
    65701 cgagcgagta gatcatcact acggccccaa catgctccgg acgcaggccc ttgactatct
    65761 ccagataaaa ggggacgagg aagttacctc ccgccaggag catcagggca aacgaggtga
    65821 ttgtgatggc cagggtcagg ttccagtcgc ggaacagcgt caaattcaaa atcggatccg
    65881 aagccttatg ttcctgatag aagaaggcga caagggagat aatgcctacc gtcagcgcaa
    65941 ttaaaatgat cctgtctgtc cagcccatat cgtttcccat actgaaggca aataccagcg
    66001 cggagagacc gatgaaactc a
//
LOCUS       DAOSMF010000004        43446 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_1128_length_43446_cov_22.948077, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000004 DAOSMF010000000
VERSION     DAOSMF010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 43446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43446
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1128_length_43446_cov_22.948077"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1313)
                     /gene="glmM"
                     /locus_tag="PLT63_01405"
     CDS             complement(<1..1313)
                     /gene="glmM"
                     /locus_tag="PLT63_01405"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOD97405.1"
                     /translation="MGKLFGTDGIRGVANEYPMTADLALNVGKATAHYFKRQGHRPRI
                     IIGKDTRISGYMLENALVSGICSMGVDAIMVGVMPTPGIAFLTQSMRADAGIVISASH
                     NPYQDNGIKIFSSTGYKLPDDTELEIEELILKNLMHNLRPSPQELGKAYRIEGARDRY
                     IVFLKSTFPKEYTLEGMKIELDCANGATYRVAPQTFLELGAEVTARFDQPNGSNINDN
                     CGSQHTEMLMEKLMKRKADAGFAFDGDGDRLIAVDEKGNVLTGDQIMAICAKAMKKKG
                     KLTNNLVVATVMSNLGLTHALKSLDIDMVATKVGDRYVLEEMRARGAAIGGEESGHLI
                     FLQEHTTGDGILSALQLLVAMKEEQQPLSELAKVMMVFPQKLINVNVKSKPDIETVPE
                     IVAAIKEVEKALDDRGRILVRYSGTQNMCRVMVEGPTNEETERYCR"
     gene            complement(1395..3014)
                     /locus_tag="PLT63_01410"
     CDS             complement(1395..3014)
                     /locus_tag="PLT63_01410"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOD97406.1"
                     /translation="MKETKFIFVTGGVLSSLGKGLAAASIAALLECRGLLVTNQKLDP
                     YINVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFTATRMKKSNNLTTGQVYFSVIA
                     KERRGDYLGKTVQVIPHITNEIKGFIRESAKDFDVAIVEIGGTVGDIESLPFLEAVRQ
                     FRLEVGKQNAVFIHLTWVPFIKTAGEVKTKPTQHSVKALREIGIQPDILLCRTENLLS
                     EDIKAKIALFCNVDVADVFAARDVSCIYEVPLIYHREGLDEKIVDLLNIWTGRPHLEV
                     WEEVVARMMHPAREASIAIVGKYVNLTDSYKSLNEALIHGGIANDCRVNLKFVDSEKI
                     EKDGLTHQLDGADGILVPGGFGDRGIEGMIQAAHYARTRNIPYLGICLGMQMAVVEYA
                     RHVCGLERANSSEFDPHTPNPVIDLLPEQKQIKEKGATMRLGAYPCRLLESSLAFTAY
                     GREEIEERHRHRYEFNNVFKEIMTAGGLRITGASPDGRLAEIVELPDHPWFLGCQFHP
                     EFKSRPTHPHPLFKDFITAALDYSDRRRGTE"
     gene            complement(3011..6778)
                     /locus_tag="PLT63_01415"
     CDS             complement(3011..6778)
                     /locus_tag="PLT63_01415"
                     /inference="COORDINATES: protein motif:HMM:NF024912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOD97407.1"
                     /translation="MSLKQFAGTFFWMWNWRVYIGRAPAELPPRSLIIFPVLNTTLPC
                     GLAGILAINKGQTAEVDVDFTASLKANFRKVREKGLPALLAGKATAPGYLGGEEFLAE
                     LDRIVLSRKDIEPFLQLLTAPDQTENTALAAEKMRRFTVAEENLLEENALRFTTAEIE
                     ILNSRLSLLKDIVWSLERDILENINRIEKLAGGPEASPTPTALKKYLQLNYLLNALDR
                     LEVRGRDSAGIQIDLTIADPESWSAAVEKLKEQGLYDDFQARSGAGDFINGTIHCSCR
                     RPEPPERKENRALAPDATGFGAAAVSLSFVYKTAAVIGALGRNVRELRNIISRDRVLQ
                     LLASLNTAGDVSLAHTRWASVGSITEENCHPVNNFSRRDGPVGGAEKHYPAYGLGNWS
                     VSVALNGDIDNYRPLRDALEAGGEFIAPAITTDTKIIPLEIERHLRSGCDAAEAFRRA
                     VNGFEGSHAIAMTTNVEPGKAFLALRGSGQSIYVGLAGDRYLFSSELYGLVEGTPYFL
                     KMDGEETGQIFILGTGDSGGPEGIRACTYGGTPLAITPALVRRAEITTRDIDRGDHPH
                     FFLKEINEAGTSIRKTLRGKYELREEGDGRRAVRFNLGPDIVPPAVRQSLATGALKRI
                     IVIGHGTAAVAGEAIAGACSRYLAESGIEITARVASELSGFDLAERLADTLVIPITQS
                     GTTTDTNRAVAMAAERGARIIAIVNRRQSDITARSHGVFYTSDGRDIEMSVASTKAFY
                     SQIVAGHILALSFAQLLKTMSDEDISGELEIIARAPEMMGRVMERRDQIRRAAETLTG
                     KKKYWAVVGSGPNKAAADEIRIKLSELCYKTISSDIVENKKHIDLSAEPLILVCAAGS
                     PPAVIGDIVKDVAIFKAHKAGIVVFADEGEERFNEVAEAVITVPRAPLPLPVILNTVA
                     GHLWGYYAARSIDAEADYLREFRSRMHQAMIEQGKRSYSLYDRLFDRQFRRLISEFAV
                     DFQERLLQGAFSQVNVRTIANIVLLLKYAVGKLPLEDFWLDFQGEEEFTSPLDLLDVS
                     LGHAIDELSRPIDAIRHQAKTVTVGTSRAEEAISGPIFQLLRDLGFPARSLTGKNIMA
                     LKALQPALASISGYTLYGIANLDAEGHPEEDTLIEILRREGTATRMSSRVAGSKKLMG
                     TKRTIVSMGDIYAGFGKADGATIVIAPLLGNNFQVEKLLLLHITYRPDMTAAEKKEVL
                     GYRYNDIRNLINEYNLPWSDACLDALPMEMLLGQPVEYIADLIKKKIEEPS"
     gene            complement(6804..8051)
                     /locus_tag="PLT63_01420"
     CDS             complement(6804..8051)
                     /locus_tag="PLT63_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine pyrophosphorylase"
                     /protein_id="HOD97408.1"
                     /translation="MSTDPKIRLLLEKGVSMPNPATVEIGPEVAIERIAGEGVIFYSG
                     TRIHGEKTLIMPGVKLGCEAPATILDCQLGPRVELKGGFFNRSVCLEKVNLGSSAQVR
                     EGCLLEEEANGAHAVGLKQTILFPFVTLGSLINFCDCFLAGGTSRKNHTEVGSSYIHF
                     NYTPNQDKATPSLIGDVPRGVMLNQPPIFLGGQGGIVGPTRIGYGTVISAGTVYRGDF
                     PEGGKLLGQTGNMAYEVNFHMGFYGDIRRRIHNNIIYLANLLALRQWYILVRRPFFVA
                     RELGRELYAGALDTLELAIGERLSRFRALAEKMEKSITISERILSGTRRETLIRQKRE
                     FMEKWPAIEACFTDGSEAEREDQNRTFFLAAMEIRGHEHRDYLQTIQNLDEHTVKAGT
                     DWLQNIVDNVINRVLDCIPACRG"
     gene            complement(8071..8367)
                     /locus_tag="PLT63_01425"
     CDS             complement(8071..8367)
                     /locus_tag="PLT63_01425"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOD97409.1"
                     /translation="MKDNHKETPLVGNKARVTSLHAVAEDILSQKLADIVTILGDTTA
                     AKSRVYEDVTAIVERCLFRIALERSQHVKSRAADYLGINRNTFHKKMTKLGLER"
     gene            complement(8394..8915)
                     /locus_tag="PLT63_01430"
     CDS             complement(8394..8915)
                     /locus_tag="PLT63_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97410.1"
                     /translation="MSEGTYRIRRSFIIPLGIDVLLLLFLLIVSLAYKGSGTEKIVLT
                     VFAGLSLLIFLEAMNRSVSVSESGVALKKLLKSRELLWSDITHVGCLKVRSRVYILLT
                     TKKGFHIISNAYDSFSTLVGEIIRHLDGGKIEIEAEAREQIENPTRNISDLVAVWVAA
                     VVLLVIIYMKFTP"
     gene            complement(8918..9397)
                     /gene="moaC"
                     /locus_tag="PLT63_01435"
     CDS             complement(8918..9397)
                     /gene="moaC"
                     /locus_tag="PLT63_01435"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018465116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HOD97411.1"
                     /translation="MVKLTHVDERGRAKMVDVSGKEPTAREAVARGRVVMKPETLALI
                     ADGGIPKGDVIGVARIAGTMAAKETGRLIPMCHPLELTGIDISFTDNRERGEIAIEAR
                     VRTIGRTGVEMEALTAVSVAALTIYDMCKAADREMTISDVMLVAKSGGKSGSFTRKD"
     gene            9611..11140
                     /locus_tag="PLT63_01440"
     CDS             9611..11140
                     /locus_tag="PLT63_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOD97412.1"
                     /translation="MIVKVLGSSVIGIEGYLIDVEVYTAPGLPQFSTVGLPDVAVRES
                     RERIKAAVKSSGYPFPRHHVTVNLAPADIRKEGTGFDLPIAVGILAAEDVVRPDLLGA
                     YLLLGELSLDGRIKGVHGALPAALKTRDLGLRGILVSPENASEAALVAGIEAIPVASL
                     TEVVEFLNGISEIKPARRDDRDIISGGEDEDCDFRDVQGQDQAKRALEIAASGAHNIL
                     LLGPPGSGKTMLARRLSSILPALSFAEAVEAAKIYSVAGLLNRGEVMIRRRPFRAPHH
                     TISDAGLIGGGATPRPGEISLAHHGVLFLDELPEFRKNSLEALRQPLEEGTVTIARAA
                     TTVVYPARFLFVAAMNPCPCGYYGDARQTCRCSAQQIRQYQRRLSGPLLDRIDLHIEV
                     PSLRYRELVGRYQGESSAVIRERIEKARAIQERRFAGKMTPFNARMSDAEVKRYCRID
                     EDAQQLLEMAMEKLGLSARAYKRILKVARTIADLDDRELLEASHVAEAIQYRNLDRKL
                     V"
     gene            11258..>11697
                     /locus_tag="PLT63_01445"
     CDS             11258..>11697
                     /locus_tag="PLT63_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97413.1"
                     /translation="MEIMIVQADPAKRKAKPKDESKLGFGRVLTDHMFTMSYHSSRGW
                     YDPVVRPYEELRLDPAAMCLHYGQLIFEGMKAYRGKSGEIYLFRPTENIRRMNESARR
                     LCMPTLDPELFLEGLKKLVLLEQDWIPKSSGASLYIRPTMIATEA"
     assembly_gap    11698..11797
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11798..12486
                     /gene="ilvE"
                     /locus_tag="PLT63_01450"
     CDS             <11798..12486
                     /gene="ilvE"
                     /locus_tag="PLT63_01450"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:TIGR01123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HOD97414.1"
                     /translation="KSSGASLYIRPTMIATEAALGVHPADAYLFFIIVGPVGAYYPEG
                     FSPTKIYVVEEYVRSAPGGIGYCKAAGNYAASLMASAMAIEKGYTQVLWLDAVHRKFV
                     EEVGTSNIFFVIDGELITPPLGGTILPGVTRDSVLQIARSWGLNVSERPLSMEEILTA
                     FNNGSLQEAFASGTAAIVSPIGQVCYQNREYAINEGRTGSLTERLYEEILRIQYGEKE
                     DPFGWRLRIA"
     gene            12789..13472
                     /locus_tag="PLT63_01455"
     CDS             12789..13472
                     /locus_tag="PLT63_01455"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HOD97415.1"
                     /translation="MYRNLFLIGVIIVAAAVLIGVSGPSAWGESPALEEVIRGLQTAY
                     EKTTDLKASFVQEVALASVNRKEREEGTVYYKPPRRMLWDYTKPAVKKLIINPDKAYL
                     YVPKDRAVYVQETARIFKSQLIVRLLDGLGKVTEDFQLAFADPGAPTDKNGNYLLVMT
                     PKKADLGVDKIFVTLSHEDFTIIQCRFADTFGNRTTVLFRNIQRNIKLPDKLFQFKTP
                     PKVEVYPLS"
     gene            13495..15717
                     /locus_tag="PLT63_01460"
     CDS             13495..15717
                     /locus_tag="PLT63_01460"
                     /inference="COORDINATES: protein motif:HMM:NF017964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HOD97416.1"
                     /translation="MGIILGINQGEDGFLPAHSDEEWLEVNGRGGYASSTLLNCHTRK
                     YHGLLVAQLAALPGRYVLLSKFEDTIIRGGRREALSCHKYPGVFFPAEYSFLKEFQQD
                     ICPSFLFSGGGLRIRKSVLLVADEDSLLIRYDPEACPASGVLRLRPFVAYRDYHHLSK
                     ENPFLKGNADAIKNGFSLRPYAGMPPLVIQTNLRPRWQPEPLWYHNFEYAEEAARGYE
                     HHEDLFSPGLLEIPLRQGKAVLVLVSLTPFYGRLSKKWDDEVRRRREVRDKDERTVAI
                     LAAGGSLTGRQFPGMGEESAASGGDAGGETKTEGDRMSPGNAAAGTAGKSPEREIVAA
                     VALEKRDIAISETDSAGIAAERELLGILLQAGRRFLIRTAAGHPAIIAGYPWFGEWGR
                     DTLLSLPGLAFCSGRRREGLEILVRMARFERQGLLPNVFSERDADHAYNTVDAPLWFF
                     WAVQQLLLDDAGDRTQAVVEKNLWPVMKNILRQFIHGTIFNIYMDDNGLIHAGAENRA
                     LTWMDACIDNRPVTPRHGYPVEVNALWYNAVHFAADLASRFGDREFYFDDLIEKIRRS
                     FLEIFWNDDDACLGDVYQSGCLDRSIRPNQIFAVSLPHSPLAPTQARLVVKKVRDELL
                     TPVGIRTLSPRDPRYRGRYAGSPAERDGAYHQGTVWPWLWGPFGEAWLKVAVDREGVK
                     RTLRDGLRTFLTGHFRMAGIGCVSEVFDGDYPHRPGGCIAQAWSTAEIIRLYTLIGGS
                     "
     gene            15756..17309
                     /locus_tag="PLT63_01465"
     CDS             15756..17309
                     /locus_tag="PLT63_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97417.1"
                     /translation="MTGNGSRAGGNRFWGETTAFLALFLDNVGVLIFLSAILVFTFNY
                     PAEIVLTRMIPGTAVGVFCGDLIYTWLAIRLRKKTGRNDVTAMPLGLDTPSTIGIAYA
                     VLGPAYVATRDAELTWHIGMTTLFMIGAVKIVTSFCGAWLQRVIPTAGLLGPLAGIGL
                     LLLGFLPLLDVFGEPIVGLTALGIVFAVILARLELPGRMPGVLVAVVVGTLIHLVLGY
                     GGYLPEFRPPSLEMKLYIPVFSLDFIKSLPQSLPYLSIAIPFGIMTIIGGIDNTESAR
                     LAGDAYRSRDILLTEAFTSLIAAFFGGVAQTTPYIGHPAYKKMGGTYWYTLFTGVLIG
                     VGAVVGLLSLFVAVIPRAALAPIFMFIGFEMTRQAYQEAPVHHSPAVSICFLPVAANM
                     MLIILGQFLGPLAVAPDTLPPRLQILHQNLTVLSNGFIVTGLLWGSMLAFLIDGKKHR
                     ASLCAVICALFSLCGIIHSVLPSGEIYPPWRPAGYAHYFIAAGYGLLAVVFAVLRPGK
                     VGDDKIFKE"
     gene            17445..18317
                     /locus_tag="PLT63_01470"
     CDS             17445..18317
                     /locus_tag="PLT63_01470"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOD97418.1"
                     /translation="MPYYVTDDHVRLYYEVRGKGRPVVLVHGLTANRRHFRKQTPELA
                     GEFQVITMDLRGHGDSEVPEHGLTLPRLARDLRELTEYLELTDLSMVGWSLGAHVIFE
                     YVKQYSCRGIAKVAVIDMAPRLLRAPDWPWGLPGVFSRKTGDFGPEDNLVMLGAMLGD
                     WEAYSRIVAQRILNKSLFNDRMEFDDRGDFKGKADLPWLYAEARRNTPHVIIALWISL
                     SLQDYRPVLKEITAPCLLAYGMESNYYPPENYAFMAEAMPRARTVPFAGCGHALHIQD
                     PERFNRELIEFLRE"
     gene            complement(18441..18983)
                     /locus_tag="PLT63_01475"
     CDS             complement(18441..18983)
                     /locus_tag="PLT63_01475"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOD97419.1"
                     /translation="MGTLIIALLGRNLAWRSFLQSLNDTLRISCMIMTIVAGATVFGH
                     FLAVTTIPTEVGTWVAGLSLPPAAIIGMIICVYLFLGCLMDSLAMIMLTIPVFYPVVT
                     TLGYDPIWFGVIIVLVAEIGVITPPVGINVYVVGGVARDVPLHTIFQGALHLLSAMVI
                     LSLLLIMFPEIALFLPGLMN"
     gene            19010..19189
                     /locus_tag="PLT63_01480"
     CDS             19010..19189
                     /locus_tag="PLT63_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97420.1"
                     /translation="MSAFWPSTWDTRAAMGTAETWAEPIRGLIFSFRNRFMSLAMSTP
                     PDGTEGEGDEAEDKD"
     gene            complement(19516..20244)
                     /locus_tag="PLT63_01485"
     CDS             complement(19516..20244)
                     /locus_tag="PLT63_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPR1/FUN34/YaaH family transporter"
                     /protein_id="HOD97421.1"
                     /translation="MAHEHSFASPGPGGLAALAVACFGFGAVFLGKVDVSGFPLLSAW
                     LIGGGIVQYTTAVMELKDHNITGGNVFLFFSAFFMFAAALSVFAKWYSISFIFIPKAA
                     KESMDAAMAAAGVTDVKMLTPEQLAAAKAGIGKAVGAMVGKAVHVEGWLWMAGAGFLT
                     AVTPAYAKSSAAMFILVILVDIVLWCIVGMDSGWFGDPKVLKPFAGWLLIIVGWIGVY
                     MAGAITCNTVYGKAIYFVPKPLVK"
     gene            20759..22345
                     /locus_tag="PLT63_01490"
     CDS             20759..22345
                     /locus_tag="PLT63_01490"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOD97422.1"
                     /translation="MEASGNEFFRQATIRLCSSLEIEKEMFNCLSYLRNYLPAREMLL
                     IIFDPIPGLIHNLARVSLEGIGKPFRSKSLSEEAICRINYDTATWQEVKILDRLELHP
                     AGWILYDHVNLSMTSMMYISLVIEGKILGSMVFLADGRGMFKREHADLISQLREPFTI
                     AVSNALRYRELTKLKDILDAENQDLTRELRYVTEDDIVGADFGLRQVMEMVRQVASLK
                     SPVLLLGETGVGKEVIANAIHYASPRRERPLVKVNCGAIPESLMDSELFGHERGAFTG
                     AIAQKKGRFERADKGSIFLDEVAELPPSIQVRLLRVLQNGEIERVGGAETIPVDIRVI
                     AATHRNLEEMVRNGNFREDLWFRLNIFPIMIPPLRNRQGDIPNLIRHFMEKKARELKI
                     HTPPPMSAQVLERLKEYHWPGNVRELENLVERELIKSRGTNGNRFMTFEHFRWPRRSG
                     PAWGAPEDNGNFLTMDEAMTHHIQLALEQTKGKIYGSDGAAALLGINPNTLRSRMKKL
                     GIFPPGSQNRRRLREGAVRG"
     gene            22438..23445
                     /locus_tag="PLT63_01495"
     CDS             22438..23445
                     /locus_tag="PLT63_01495"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOD97423.1"
                     /translation="MKLIYATDVHGSFERVRELLRLTTADVYIIAGDLVDIPFYTVEK
                     ARRYHDLQTYFHSLRRDRGKTAATLEDFVATLLEEPDLPGEVWEKGDRFRYETMRARH
                     ILQKHYRLLDNILSLATAGTAFALPGNHDMDLHHTLLRGRDLHLQCHESKGIKIAGYG
                     GAAGNTPGIPERFGVAYREGGGGKNDEMYRFFQSVRPDVIAVHQPPYGIHDFAPPMGE
                     TGSHALRRYCERHPVLLCLTGHLHDQWGLEEVEDTVFLNPSHFGEIVQANGTVAEGGF
                     FHELEIDGGKIGGVRHRKLVGDLIHDLVYYERREGEWARRVVDRERYGALLRGINCEP
                     L"
     gene            23482..24591
                     /locus_tag="PLT63_01500"
     CDS             23482..24591
                     /locus_tag="PLT63_01500"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOD97424.1"
                     /translation="MTRKGEADVRLLGRMANLAISCLTGEEVVAVAARFLPQLFPGES
                     GALFLRPAAGKEYEPAASWGEERAWRPFSGADCWSLRRRRLHSVRQGKDQPLCPHADH
                     DPERWISLCIPMTAREDVLGVFHVLIPVEESSTDAGKGPGDRLLQGGSPIPAAGSETG
                     EAGELGGSDTYLGEGADERRLAALTADILTMIFVNLRLRRQIDDLAVRDALTGCFNRR
                     YLEDSLARELKVVQRTGRPLAVVLMDIDHFGRFTDAFGHGSSSLVLRELGEFLRNNVR
                     DVDLSCRYGADEFALLFPDTDPPGARRRAEHLQAMLRDLSRHGGGSHLRRITLSMGVA
                     AYPDQGETWGDLMEAVKAALKRAKQTGRDRVCGGE"
     gene            24675..25937
                     /locus_tag="PLT63_01505"
     CDS             24675..25937
                     /locus_tag="PLT63_01505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOD97425.1"
                     /translation="MRVLMFGWEFPPLVSGGLGTACHGITRALVRLGHQVIFVMPHGE
                     GDAAFPGLTIVSASAVLGALAAARTFPSPVDDAPDLLPARLVLHPYLTVRPPKDGVPA
                     FRKWPADSILAGSGGYGADLFTEVGRYGEAGRIVAARTSFDVIHGHDWMAVPACLAAR
                     RVSGRPFVLHVHSLEYDRSGAGGNGEICAVERRGLEAADRVIAVSNYTKEMIVRHYGI
                     AREKIAVVHNAVTAAEGRKAYRVPAGNGEKRVLFLGRITFQKGPEYFVEAAARVLKEM
                     PETTFIMAGSGDMMPEMVEKVAELKIGRRFHFTGFLQGRQVEEAFAGADLYVMPSVSE
                     PFGLTPLEAMMYDVPVIVSRQSGVAEVLKHAVKVDFWDVAALAEKMIAILKYPPLAAE
                     VVARGREELQGLSWEAAARKIVAVYKSL"
     gene            26075..27331
                     /locus_tag="PLT63_01510"
     CDS             26075..27331
                     /locus_tag="PLT63_01510"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HOD97426.1"
                     /translation="MAVDAGAAWRADTRRATGGDERMTAVCLCFQVHQPRRLRRYPVF
                     AIGRERNYEDEAGNRKILDKVVDKCYLPANDLLLSLIDRHGGKFRLSFSITGVLLDEL
                     ERFHPEVIASFQALAGTGCVEFLNETYHHSLAFLYSDEEFRGQVDRHRRRITELFGQE
                     GETFRTTELIYNDALAAAAAEMEYRAILAEGAAAVLGPRDPNRVYEAAGGGGIRVLPR
                     NYRLSDDISFRFSRRGWEGYPLTAAKYARWLHRLHGPETVINLFMDYETWGEHQWRET
                     GIFSFLEALPGAILAGADFVFVTPREAAARKPAGVLSVPGFCSWADTERDLTAWRGNA
                     MQRDALEALYGLEEAVTAKGDRDLAEQWRQLQTSDHFYYMCTKWCADGDVHRYFNPYP
                     SPYDAYINYMNILDDFSRRVGKEKGS"
     gene            27328..31584
                     /gene="glgP"
                     /locus_tag="PLT63_01515"
     CDS             27328..31584
                     /gene="glgP"
                     /locus_tag="PLT63_01515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOD97427.1"
                     /translation="MTEVRNGYLFEVSWEVCNKVGGIYTVIASKLREALKVYGDRYIL
                     LGPDLKTNVDFEETDEDCWAKIREGTAIKDIPCRFGRWKISGEPKAILVSAGKKYDKD
                     QLLFRLWEAFGVDSIAGGWDYVEPVMFSFACGEVIETIYNLAVRPYNLAAVAHFHEWM
                     TGAGLLYLKKRVPEVGTVFTTHATILGRSLAGAGVDIYGMMEHLAPQREASAHNITAK
                     YSMEAVTAREADCFTTVSEITAAEAKNFLSRTPDVITPNALDMENIPDYALSRAPAER
                     SREKLLAAAARFLRRELPRETRILIISGRYEYHNKGIDIFLEALGRLEKEGRGGPGVL
                     ALLFVLGGYTDLIPALRDDTGRSETGKSPLVTHRLHNEGADPILQACNRLGLTNQPGG
                     KVQVIFNPAYLNGHDGLINMSYYEALSGCDLGVFPSYYEPWGYTPLESVAYGVPTVTT
                     DQAGFGLWCERTCEENGGVMLLRRRGRPRPSIEDHLFRIVGDFLDWTPEEMAAHRRSA
                     REVALRANWRDFFDYYLQAYDRAGSRALNRAEQAAAKAERAEVRHVYAGAVSAQPHFR
                     SFTAVANLPEQIIRLRELAYNLWWTWNPRALDLFATLDLKLWAEMGNNPVRMLETVSP
                     DILREAAANASYLNLYNRIMQQFDEYLGEKGPRRRLPATAGLRWSSPVAYFSAEYGLH
                     ECLPIYSGGLGTLSGDHLKTASDLNLPLVAVGLLYKNGYFRQMIDEQGRQTEAYPEND
                     FSSMPVRIVQDDKGNEVQIAIDLPGRTLFANIWEVKVGKVSLYLLDTDVPRNTQQDRA
                     ITARLYSADQKMRIEQEILLGVGGVRLLKKLGIRPRVYHINEGHSAFLIFERIETLMK
                     EEGLTFEEAAEVVRASSVFTTHTPVEAGNERFSREIMEHYFSGFIKRSGLSWVQFWEL
                     GRRDGGEEKNFFMNILAFKMTIRSNAVSLVHGQVSRRMWRDVWKGFDDTDIPIRHITN
                     GVHVLSYLAPRMRNLLDAYLGMDWEKILTDAERWSRIQDIPDALLWRVRYELKQKTID
                     LIREDISKGGAKYGTAHVWREELFSRLNPGALMIGIARRFAPYKRADMILADLDRLDR
                     IVNHPHRPVHLVFAGKAHPNDGVGKHLVEKVVGICRDDRFRGRIFFLENYDIRVARHL
                     VQGVDLWLNNPRRPLEASGTSGMKVVINGVLNMSVSDGWWVEGYNGANGWTIGPVLKG
                     YEDPPADADAEDGQSLYNILENMVIPAFYDRDAAGVPVKWLATVKQSMQSLAPQFNTD
                     RMLQEYLRNMYGPAARRECELAADGYDLARRLADWKGKLPMRFSSLKLIDVSVEGIHG
                     DTVMVDQPLKISARIDPGKLAPEEILVEIMIGRRNGHDFVRPPECVPLEIAGRSPEGI
                     LTFSTEYFVRENGLYNYGVRVIPRHEHLASKLETGLILWG"
     gene            31727..33829
                     /gene="pta"
                     /locus_tag="PLT63_01520"
     CDS             31727..33829
                     /gene="pta"
                     /locus_tag="PLT63_01520"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF004167.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HOD97428.1"
                     /translation="MSKGVFIASVEPRSGKSLFVLGMMNAIVNQRGCVGYFKPVIETD
                     GRGAKDAHVEMIRKHFDLPFEYEALFGFRRHEIAALLTRGEDYHVVATLLDKYKQIED
                     ACDYTIVEGTDFLGKNTFFEFDANVALPKNLGIPAIIVVSGEDKTPDEIKGGVLGAYG
                     GYREGDVEVLAVAVNKVHSDAVPLIEPLLRPMLSAETVLAVIPENPKLKNPTFREVSA
                     ALGARTLFGRHRHENRVDHTVVGAMLISNFLNHLQDNTLIVTPGDRADIIVGALQANI
                     SRNYPRLAGIILTGALDMDPAIVKLIEGLQTVAPISAVTTGTYETANRVGAVHSRIYA
                     GNARKIALAMDEFDRYMDMEKLGEKMATFKKAGVTPYMFKYGIVKRAKGRRRHIVLPE
                     GDDDRILTAASRLVEQEVVDLTILGVRENVLTAGKRLGVNLEWEEIRIVDPVRSEYYQ
                     DYAATLQELRKEKNVTIEMARDLMTDVSYFGTMMVYKGHADGMVSGAGHTTQHTIRPA
                     LQFVKTRPGVSVVSSVFFMCLPDRVSVFGDCAVNPDPTAEELAEIAICSAESAAMFGI
                     EPRIAMLSYSSGTSGQGADVEKVRRATEIVRARRPELKIAGPIQYDAAVDPAVGKQKM
                     PDSDVAGQASVLIFPDLNTGNNTYKAVQRETGAVAIGPILQGLNKPVNDLSRGCTVED
                     IFNTVVITAIQSQEGMTD"
     gene            complement(33923..34714)
                     /locus_tag="PLT63_01525"
     CDS             complement(33923..34714)
                     /locus_tag="PLT63_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOD97429.1"
                     /translation="MTKILRKIIQIDEELCDGCGNCVPGCAEGALQIIDGKARVVAES
                     YCDGLGACIGDCPTGALRLIEREAAPFDEEAVARRHPEAGAADTLPCGCPSTFIQILG
                     PGVSCTEANRPREISAGQGPGRKEEGGGDGEPRAWRGESSLTHWPVKIRLVPPHAPFL
                     KGADLLVLADCTAVAMPTLHRDLMKGKVVLMGCPKFDDVQGYTEKFADIFRTADIRSV
                     TMAVMEVPCCSGLPMIVKKGMAAAGRDIPEEVVVIAAAGNIRKRS"
     gene            34893..35582
                     /locus_tag="PLT63_01530"
     CDS             34893..35582
                     /locus_tag="PLT63_01530"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOD97430.1"
                     /translation="MEDARKALSASQLFGGLPEADLAAIEKIAAVKHFDKGEIIFFEG
                     DEGVGFYLVALGRVSVFKLAPDGREQTLHLVKEGDAIGAVPVFSGKSFPANARALTQC
                     RLIFIPREKFIQLIADKPLLTMNLLALLATRLREFTIQVENLSLKEIPSRLAAYLLYL
                     SREQGGSDLIGLSVTKGQLASLLGAGPESLSRALGSLKARGLVAEDGEGIRLINRTLL
                     TELAESGRDFR"
     gene            35775..36260
                     /locus_tag="PLT63_01535"
     CDS             35775..36260
                     /locus_tag="PLT63_01535"
                     /inference="COORDINATES: protein motif:HMM:NF023583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HOD97431.1"
                     /translation="MAKDNNNGANLGFESDLWRAADVLRSNMDAAEYKHVVLGLIFLK
                     YISDAFEEQHARLEADSAQGADPEDPDEYRAVNIFWVPKEARWSRLKSEARQPTIGKV
                     IDDAMLAIERDNPSLKGVLPKDYAHPRLDKQRLGAHRHGGQHRPGRQGQPLQRYSRPG
                     L"
     gene            36193..36729
                     /locus_tag="PLT63_01540"
     CDS             36193..36729
                     /locus_tag="PLT63_01540"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOD97432.1"
                     /translation="MVGNIGLGDKVNRSKDILGRVYEYFLSQFASAEGKKGGQFYTPR
                     CVVRVLVEMLAPYKGRVYDPCCGSGGMFVQSVEFVKAHATGNGNGGKTKADVSIFGQE
                     SNHTTWRLAKMNLAIRHHNHILTPGRYVGAAEVEDDGEPFEEKMARLTTALREQTGKA
                     EKLDRIIWANLEDVGYGG"
     gene            36719..37864
                     /locus_tag="PLT63_01545"
     CDS             36719..37864
                     /locus_tag="PLT63_01545"
                     /inference="COORDINATES: protein motif:HMM:NF036995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HOD97433.1"
                     /translation="MGDKKPAKADGFAVLLSEVKQRIQLAQTRAVLAVNSELVRLYWD
                     IGRIIDLRQKREGWGAAVIPRLAQELKNEMPELKGFSERNIGRMIAFYREYADPSAFL
                     PQAAAKISSPAKVPQPAAKSGNVLLWSIPWFHHVVLMEKVKDIPTRYWYMEQALTNGW
                     SRNVLTLQIEAQAHARHGKARSNFALTLPPHQSDLVQQTLKDPYIFDFLTLTEPFHER
                     ELETELVRHLEKFLLELGQGFAFVGRQYKLDVGDEEFYIDLLFYHLRLRAFVVIELKK
                     GKFKPEYAGKLNFYCNVVNDRLKQPADQPTIGLMLCQERSRLLAEYSFVGIDKPIGIS
                     TYKLTRALPRELRSALPTVEEIEAELAEVVKQKPKGTGGRKKKGGGE"
     gene            38130..38705
                     /locus_tag="PLT63_01550"
     CDS             38130..38705
                     /locus_tag="PLT63_01550"
                     /inference="COORDINATES: protein motif:HMM:NF022018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HOD97434.1"
                     /translation="MGIKAVNQPSVKAWGKAMKSLVPVETIEKKILLIRDEKVMLDSS
                     LAILYGVETREIVQAVKRNIGRFPDDFMFQLTAEEFDLLRSQSVISKPVGRGGRRYLP
                     YAFTEQGVAMLSSVLKSERAIKVNIEIMRSFVNLRRMLSSNAELARNLAALEKKYDLQ
                     FKAVFDAIRQLMAPPVNPKRKIGFHADRIDK"
     gene            38794..39972
                     /locus_tag="PLT63_01555"
     CDS             38794..39972
                     /locus_tag="PLT63_01555"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HOD97435.1"
                     /translation="MAGEWVNKTLGDVLTLQRGFDLPETEREAGIFPVIASTGRVGTH
                     SVSMVKGPGVVIERSGSLGGGQFIQSDFWPLNTTLRVKDFKGNDARFCYYLLKSLDLA
                     TFNTGSGVPTLNRNHIHPLPVQVPSDVAEQRAIAHILGSLDDKIELNRRMNRTLEEMA
                     RAIFKSWFVDFDPVRAKAAGQQPPGLKPEIASLFPDSFEDSELGEIPRGWRVLPIGEL
                     AEVSSGKRPNARFAERTDEASVPLWGGNGPMAFVRKALFDEPVLLTGRVGTLGSVFRI
                     TEPCWPSDNTLVLQATEPSVFQYLFLQLHQVNFTSLNRGSTQPLLTQTDLKAQRLVVS
                     TKSILETFSRIADSMYCRFDESTAEILTLVAIRDTLLPKLISSELRLPDAERITERCL
                     "
     gene            39969..40247
                     /locus_tag="PLT63_01560"
     CDS             39969..40247
                     /locus_tag="PLT63_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97436.1"
                     /translation="MNENQQFLLYTAPDGAVKVEVFFKGETVWLTHKALAELFGVKTP
                     AVNKHLKNIFESGEMAEDSVISILESTAAGGKNYQTRDWRVKSEPRIQ"
     gene            40228..40866
                     /locus_tag="PLT63_01565"
     CDS             40228..40866
                     /locus_tag="PLT63_01565"
                     /inference="COORDINATES: protein motif:HMM:NF016220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HOD97437.1"
                     /translation="MSPAFSESVIEQVALAWLESLGYQVLSGLEIAPGEPAAERDNYG
                     QVVLEYRLRQALAWLNPQVPADALEEAFRKLTRPDSPALVANNHAIHKYLVEGVPVEY
                     QRKDGSIGGDLVRVLNYDEPEDNEFLAVNQFTVVENQIERRPDVVLFINGLPIAVMEL
                     KNAATENATIWSAFNQLQTYKMQIPSLFSFNEAMVISDGVQSRIGTDRHADG"
     gene            40910..41506
                     /locus_tag="PLT63_01570"
     CDS             40910..41506
                     /locus_tag="PLT63_01570"
                     /inference="COORDINATES: protein motif:HMM:NF037510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOD97438.1"
                     /translation="MADTKLPQLQVMLEGVFEKRRLLDLIRHFIVFEDVGGGVLVKKM
                     AEYHQYHAVNVAVQETVRACMTVADVTGIRDEGTYFTRGQRDAKRGDRRVGVVWHTQG
                     SGKSLKMAFYAGRVILHPAMENPTLVVITDRNDLDDQLYGTFARCHELLRQQPMQAQS
                     RAHLRELLTTASGGVVFTTVHKFFPSEGEDQHPLLSDR"
     gene            41516..41902
                     /locus_tag="PLT63_01575"
     CDS             41516..41902
                     /locus_tag="PLT63_01575"
                     /inference="COORDINATES: protein motif:HMM:NF023295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HOD97439.1"
                     /translation="MIADEAHRSQYDFIDGFARHMREAGRRGEKLGLTDDEVAFYDAL
                     EVNDSAVAVLGDETLRMIAQELVKAVRNSITIDWTVRENVRAQMRVIIKRILRKYGYP
                     PDKQARATELVLEQAEIICKDWSEKI"
     gene            41899..42144
                     /locus_tag="PLT63_01580"
     CDS             41899..42144
                     /locus_tag="PLT63_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97440.1"
                     /translation="MTNDKEVKTFEKPRSGVHIVIPVRSGIQYYQEVPGFRIKPRMTE
                     QGVFQRSQVVIADVSIGLDRLVRGPFVLPGRDMVISW"
     gene            complement(42131..42358)
                     /locus_tag="PLT63_01585"
     CDS             complement(42131..42358)
                     /locus_tag="PLT63_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HOD97441.1"
                     /translation="MMNDFSQLGKILLIVGLAVAAVGLLIMLGGKLPWLGRLPGDIFY
                     RGEKFTFYFPLTTSIIASIVLTLIIWLITRR"
     gene            complement(42366..43310)
                     /locus_tag="PLT63_01590"
     CDS             complement(42366..43310)
                     /locus_tag="PLT63_01590"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOD97442.1"
                     /translation="MFVEKITPYYRNLIDHDGPDGPLARMVRHSELERRVRPEERTDP
                     IGDGRWQRGANGRLIHHYPGRALLLVTDRCAAHCRFCFRRDRLSRFSSDLTPEELAAA
                     CAYLAAHGEIHEVILSGGDPLSLADETLTAVIGAVRKAGARSVRLHTRYPVYDPVRCE
                     SFGPVAAQLDTIVVHVNHRLEITAEFLRAAAALRRARFLLNQSVLLKGVNDSEEELAG
                     LSLALGEAGILPYYLHYPDLAPGISHFRIPLEAAIGLSRALSGRLPGYLIPRLILDIP
                     GGKGKIPLAGQEGRKGDDGAYLFTAPLTGETVTYREAL"
BASE COUNT         8725 a        12572 c        13156 g         8893 t
ORIGIN      
        1 cggcagtacc gttccgtttc ctcgttcgtc ggtccttcca ccatgacccg gcacatgttc
       61 tgggttcccg aatagcggac caggatacga ccccggtcgt ccagggcctt ctccacttcc
      121 ttgatggccg cgacgatttc cgggaccgtc tcgatgtccg gcttggattt gacattgaca
      181 ttgatgagct tctggggaaa taccatcatc accttggcca gttccgaaag gggctgttgc
      241 tcctccttca tggcaacgag gagttgaagg gcgctgagga tgccgtcccc cgtggtatgc
      301 tcctgaagga agatcagatg tcccgactcc tcaccgccga tggctgcccc gcgggctcgc
      361 atctcttcca ggacgtaacg gtcccccact ttggtggcga ccatgtcgat gtccagggat
      421 ttcagggcat gggtgaggcc gagattgctc attactgtgg ccaccaccag gttgttcgtg
      481 agctttccct tctttttcat tgccttggcg cagatggcca tgatctggtc gccggtgagg
      541 acgtttcctt tctcatccac ggcgatgagg cgatccccgt caccgtcgaa ggcgaagccc
      601 gcgtcggcct tcctcttcat gagcttctcc atgagcatct ccgtgtgctg ggagccgcaa
      661 ttgtcgttga tattggaacc gttgggctgg tcgaagcggg ccgtgacctc cgccccgagc
      721 tccaggaagg tctggggagc gacgcgatag gtggcgccgt tggcgcagtc cagttcgatt
      781 ttcattcctt cgagggtgta ttccttagga aaggtgctct tcaaaaagac gatgtaacga
      841 tcccgggctc cttcgatgcg gtaggccttg cccagttcct gaggggaggg gcggagattg
      901 tgcatgaggt ttttcaggat cagttcctcg atctccagtt ctgtatcgtc ggggagttta
      961 tagccggtgc tggagaaaat cttgatgccg ttgtcctgat agggattatg ggaggctgag
     1021 atgacgatgc ccgcatcggc ccgcatgctc tgggtgagaa aggcgatccc cggcgtgggc
     1081 atgaccccca ccatgatagc atcgacaccc atggagcaga ttccggaaac gagggcgttt
     1141 tccagcatat atccggaaat gcgggtatcc ttgccgatga tgatccgagg ccggtgaccc
     1201 tgacgtttga agtaatgggc cgtcgccttg ccgacattga gggccagatc cgcggtcatg
     1261 gggtattcat tggccacccc ccgaattccg tccgttccaa agagttttcc cataagcatc
     1321 catctccttt tcgtgatgat gaaaattgac ggcgtataac ctgctgtgca ggtaaagtca
     1381 aagtatttat tatattattc agtgccccgg cgccgatcgg aataatcgag ggccgcggtg
     1441 atgaagtcct tgaaaagcgg atggggatga gttggccgtg atttgaattc cggatggaac
     1501 tggcagccca gaaaccacgg gtgatcagga agttccacaa tttccgccag tcgcccgtcc
     1561 ggggaggcgc cggttatccg caggcccccg gcggtcatga tttccttaaa aacgttattg
     1621 aattcatacc gatggcggtg tcgttcctcg atctcttccc tgccgtaggc ggtaaaggca
     1681 agggaggatt ccaaaagccg gcagggatac gcgcccaggc gcatggtggc ccctttttcc
     1741 ttgatttgct tctgctccgg aaggagatcg atgacggggt tgggggtgtg cggatcgaat
     1801 tccgaactgt tggcccgctc cagtccgcat acgtgacggg catactccac caccgccatc
     1861 tgcatcccca ggcagatgcc caagtagggt atattccggg tgcgggcgta atgggccgcc
     1921 tggatcatcc cctcaatgcc ccggtctccg aagccgccgg ggacgagaat cccgtcggca
     1981 ccgtcaagct gatgggtaag gccgtccttt tcgatctttt ccgaatcgac gaatttcagg
     2041 tttactcggc agtcgttggc gatgccgccg tgaatcaggg cctcgttcag gcttttgtac
     2101 gaatcggtga ggttgacgta tttgcccaca atggcgatgg aggcctcccg ggcgggatgc
     2161 atcatccggg cgacgacctc ttcccagacc tccaggtgcg gacgtcccgt ccagatattg
     2221 agaagatcga cgatcttttc atccagcccc tcgcggtgat agatcaaagg tacctcgtag
     2281 atgcagctca catctcgggc ggcgaaaacg tccgcgacgt cgacgttgca gaacagggcg
     2341 atcttggcct tgatgtcctc ggatagaagg ttttctgtcc ggcagaggag gatgtccggt
     2401 tggatgccga tctcccgcag ggccttgacg ctgtgctgcg tcggcttggt cttcacttcc
     2461 cccgccgtct tgataaaggg gacccaggtc aagtggatga aaaccgcatt ctgtttgccc
     2521 acctccaggc ggaactgccg gaccgcctcc aagaagggta gactttcgat gtctcccacg
     2581 gtgcccccga tttccacgat agccacgtcg aaatccttcg cgctttcccg gataaaaccc
     2641 ttaatctcgt tggtgatatg ggggatgacc tggacggtct tgcccaggta gtccccccgc
     2701 cgctccttgg cgatgacgga aaaatagacc tgccccgtgg tgaggttgtt gctcttcttc
     2761 attcgcgtcg ccgtgaagcg ctcgtaatgg ccgaggtcca ggtccgtttc cgccccgtcg
     2821 tcggttacga agacctcacc gtgctggaag ggactcatgg ttcccgggtc cacgttgata
     2881 taggggtcca gtttctggtt cgtgaccagc agaccacggc actccaggag ggcggcgatg
     2941 gaggcggcgg ccaggccctt gcccagggag gaaaggacgc caccggtgac gaagatgaat
     3001 ttggtttctt tcatgacggt tcctcgatct tctttttgat tagatcggcg atgtattcaa
     3061 ccggttgccc cagcaacatc tccatgggca aggcatccag gcaggcgtcc gaccagggaa
     3121 gattgtactc gttgatcaaa ttgcggatgt cgttatagcg gtaacccaag acctcctttt
     3181 tctccgcggc ggtcatgtcc ggccggtagg tgatgtggag gagcagaagc ttttccacct
     3241 gaaaattgtt gcccagcagc ggggcgatga cgatcgtcgc cccatccgcc ttaccgaagc
     3301 cggcatatat gtcgcccatg ctgacaatgg tccgtttcgt ccccatgagt tttttcgatc
     3361 ccgccacccg ggaagacatc cgggtggccg tgccttcccg ccgcagtatc tcgattaggg
     3421 tgtcttcctc tggatggcct tccgcgtcga ggttggcgat gccatagagg gtatagccgg
     3481 agattgacgc cagggccggt tgcagtgcct tgagagccat gatgttcttc ccagtcaggg
     3541 atctggccgg gaagccgaga tcccggagga gttggaagat cggaccggag atcgcctcct
     3601 ccgccctgct ggtgcccacc gtcacggttt tcgcctgatg acggatagcg tcgatgggcc
     3661 gggaaagctc gtcgatggca tggcccaggg agacgtcgag gaggtcaagg ggagaggtga
     3721 attcctcctc tccttgaaaa tcgagccaga agtcttccag gggaagtttg cccacggcat
     3781 atttcaacag gaggacgata ttggctatcg tccggacgtt gacctgggaa aaggcacctt
     3841 gtaacagccg ctcctgaaaa tccacggcga actcactgat cagccgccgg aactggcggt
     3901 cgaaaaggcg gtcgtacagg gaataagatc gtttgccctg ctcgatcata gcctgatgca
     3961 tccggcttcg gaactcccgg agataatcgg cctccgcgtc gatgctccgg gcggcataat
     4021 atccccagag atggccggcg acggtgttga ggatcaccgg caggggcagc ggcgcccggg
     4081 ggacggtgat caccgcctcg gcgacctcgt tgaacctttc ctccccctcg tcggcgaata
     4141 cgacgattcc ggctttgtgg gccttgaaga tggccacgtc cttgacgatg tccccgatca
     4201 cggcgggtgg gcttccggcc gcgcagacga ggatcagcgg ttcggcggac agatcgatgt
     4261 gcttcttgtt ttccacgatg tctgaagaga ttgtcttata acacagctcg ctgagtttga
     4321 tccgtatttc atccgccgcc gccttgttgg ggccgctgcc cacgacggcc cagtacttct
     4381 tcttccccgt caacgtctcg gcggcccgac ggatctggtc ccgcctttcc atgacccggc
     4441 ccatcatttc cggcgcccgg gcgatgatct ccagttctcc ggagatatcc tcgtcggaca
     4501 tcgttttcag gagttgggca aagctcagcg ccaggatgtg tccggcgacg atctgggaat
     4561 agaaggcctt ggtggaggca acggacatct caatgtcccg tccgtcggag gtgtagaaga
     4621 ccccatggga cctggcggtg atatctgatt gccgccggtt gacgatggcg atgatccgcg
     4681 ccccccgttc cgccgccatg gcgacggccc ggttggtgtc cgtggtggtt cccgattggg
     4741 tgatgggaat gaccagggtg tcggcgagac gttccgccag gtcgaaaccg ctcaattccg
     4801 aggcgacgcg ggccgtgatc tcgatgccgc tttcggcgag ataccgggaa caagccccgg
     4861 cgatcgcctc gccggccacc gcggcggtgc cgtggccgat gacgatgatc cgtttaaggg
     4921 cgccggtcgc aagggactgc cggacggcgg gcggcacgat gtcggggccg agattgaagc
     4981 gcaccgcccg gcggccatcc ccttcctcac ggagctcgta tttgccccgc aatgtttttc
     5041 ttatggaagt gccggcctcg tttatctcct tgaggaaaaa gtggggatga tccccccggt
     5101 cgatgtccct tgttgtgatc tccgcccgcc ggaccagggc gggagtgatc gccagaggcg
     5161 tcccgccgta ggtacaggcc cggattccct ccggcccgcc ggaatcaccg gtcccgagga
     5221 tgaagatctg tcccgtttct tctccgtcca tcttgaggaa ataaggcgtg ccctccacca
     5281 gcccgtagag ttccgaggag aagaggtagc gatcaccggc gagccccaca tagatggact
     5341 ggccgcttcc ccgcagggcc agaaaggcct ttcccggttc cacgttggtg gtcatggcga
     5401 tggcgtgaga gccctcaaaa ccgttcaccg cgcggcggaa ggcctcggcg gcgtcacatc
     5461 cggaccgcag gtgcctttcg atctccaggg ggataatttt ggtgtccgtg gttatcgccg
     5521 gggcgatgaa ctcgccgcca gcctctaggg cgtcgcggag ggggcggtag ttgtcgatgt
     5581 caccgttgag ggcgacgctc acggaccagt tccccaggcc gtaggcggga taatgctttt
     5641 cagctccgcc caccggtccg tcccggcggg agaagttgtt cacgggatgg cagttttcct
     5701 ccgttatgga tccgacggag gcccagcggg tgtgggccag ggaaacatcc ccggcggtgt
     5761 tcaaggaagc cagaagctgg aggactcggt cccgggagat gatattacgc aattcccgga
     5821 cgtttctgcc cagggcgccg atgacggcgg cggtcttgta aacgaacgac agagagacgg
     5881 cggcggcccc aaatcccgtc gcgtccgggg cgagagcacg gttctccttc ctttccggcg
     5941 gctccggtct ccggcaggag cagtgaatcg taccattgat gaagtcgccg gcgccggacc
     6001 gggcctggaa atcatcgtag agcccctgct ccttgagttt ttccaccgcc gccgaccaac
     6061 tttccggatc ggcgatcgtc aggtcgatct ggattcccgc ggaatctctc ccccgcactt
     6121 ccagacggtc caaggcgttg agcagatagt tgagctgcag gtattttttc aaggccgtcg
     6181 gcgtcgggga tgcctccggg ccgccggcaa gcttctcgat ccggttgatg ttttccagga
     6241 tatctctttc cagggaccag acgatgtctt tcaataacga caggcgacta ttgagaatct
     6301 caatctccgc tgtggtgaaa cggagggcgt tttcctccag gagattctcc tctgccacag
     6361 taaaacgccg cattttttca gccgcgaggg ccgtattctc ggtctggtcc ggagccgtga
     6421 gcagttgaag gaaaggttcg atatcctttc gggaaagcac tatcctgtcc aactccgcca
     6481 ggaattcttc tccccccaga taccccggcg ccgtggcctt cccggccagc agggccggca
     6541 gccccttttc ccggaccttc cgaaaattcg ccttcaggga ggcggtgaaa tcgacatcta
     6601 cttctgctgt ttgccccttg ttgattgcga ggatacccgc aagcccacag ggaagggtgg
     6661 tattcaaaac gggaaagatg atgagggatc gggggggtag ttcagccgga gcgcgaccga
     6721 tgtaaacgcg ccagttccac atccagaaga aggttccggc aaactgcttt aacgacatat
     6781 cgacaatttc agcgtttcga tcattagcct ctgcatgcgg gtatgcaatc caggacccgg
     6841 ttgatgacat tatctacgat gttctgcagc caatccgtcc ccgccttgac ggtgtgttcg
     6901 tccagattct ggatggtttg gagatagtct cggtgttcat ggccccggat ttccatggcg
     6961 gccaggaaga atgtccggtt ttggtcttcc cgctccgctt cgctaccatc ggtgaagcaa
     7021 gcttcgatag cgggccattt ttccataaat tcccgtttct gccggatcag ggtctcccga
     7081 cgggtgcccg agaggatcct ttcggagatg gtgatggatt tttccatctt ttccgccagg
     7141 gcccgaaaac ggctcagccg ctcgccgatg gccagctcca gtgtgtccag cgccccggcg
     7201 taaagttccc tgcccagttc ccgggcgacg aaaaagggcc ggcggacgag gatgtaccat
     7261 tgccggaggg cgagaagatt ggctagataa atgatattgt tatggatgcg ccgccggatg
     7321 tcgccgtaga agcccatgtg aaaattgacc tcgtaggcca tgttccccgt ctggccgagg
     7381 agtttgcccc cctcggggaa gtccccccga tagaccgttc ccgccgagat taccgttccg
     7441 taaccgatcc gggtgggccc tacgattcct ccctggccgc ccaagaagat cgggggctga
     7501 ttcagcatga caccccgggg gacatcaccg atcagggagg gggtggcctt atcctgattc
     7561 ggggtatagt tgaagtgaat ataagaactt cccacttccg tatggttctt ccggctcgtt
     7621 cctccggcga ggaagcagtc gcagaagttg atcagactgc cgagggtgac aaagggaaag
     7681 aggatcgttt gctttagacc cacggcatgg gctccgttcg cctcttcctc caggagacag
     7741 ccctcccgca cctgggcgct gctgcccagg ttaaccttct ccaggcatac ggatctgttg
     7801 aaaaatccgc ccttcagttc cacccggggc cccaactggc aatcgaggat cgtagcgggg
     7861 gcttcgcaac caagtttaac tcccggcatg atcagggttt tttccccgtg gatccgcgtg
     7921 ccgctgtaaa agatcacgcc ctcgccggcg atacgttcta tcgccacttc cggtccgatc
     7981 tccacggtgg ccgggttggg catggacacc cctttttcga gcaggagtct tattttggga
     8041 tcggtggaca tatctcctcc gtaatgacgc ttaacgttct aacccgagtt tggtcatctt
     8101 tttatggaag gtgttgcggt tgatgcccag ataatcggcg gcccggcttt tgacgtgctg
     8161 ggatcgctcc agggcgattc tgaagagaca acgttccacg atggcggtca catcctcata
     8221 aaccctgctt tttgccgccg tagtgtcgcc gaggatcgtg acgatgtccg ccagcttctg
     8281 ggagaggatg tcttccgcca ccgcatggag ggatgtcacc ctcgccttat tgcccaccag
     8341 cggtgtttct ttgtgattgt ccttcataaa acaggctttt ttatccttac tttttagggc
     8401 gtgaatttca tgtatataat gaccagcagg acaacggcgg ccacccaaac cgccacgagg
     8461 tcggatatgt tccgggttgg attttcgatc tgctccctgg cctcggcttc gatctcgatc
     8521 tttcccccgt cgagatgccg gatgatttcc cccaccaggg tggaaaagga atcataggcg
     8581 ttcgagatga tatgaaaccc cttcttggtt gtcaagagga tatagaccct gcttctcact
     8641 ttcaggcaac ccacatgggt gatgtcactc cacaggagtt ccctgctctt gaggagcttc
     8701 ttcagcgcca cgccgctctc ggaaaccgaa acactccggt tcatggcctc cagaaagatc
     8761 aggagggaca ggccggcaaa gacggtcagg acgatctttt ccgttccaga acctttgtat
     8821 gccagggaaa cgatcagcag aaagaggagc agcaggacgt cgatgcccag ggggatgatg
     8881 aacgaccggc ggatgcggta tgtaccctcg gacatgatca atcctttctg gtgaaagaac
     8941 cgcttttacc gccgctttta gcgacgagca tgacgtcgga aatggtcatt tcccggtcgg
     9001 cggctttgca catgtcgtag atggtgaggg cggcgacgct tacggcggtc agggcttcca
     9061 tctccacgcc ggttcggcct atggttctta cccgggcttc gatggcaatc tcaccccgtt
     9121 cgcggttgtc ggtaaaagaa atatcgatcc ccgtaagctc cagaggatgg cacatgggaa
     9181 tcagacgccc cgtttctttg gcggccatgg tcccggcgat gcgggcaacg ccgattacgt
     9241 ctcccttcgg gatgccgccg tcagcaatga gggccagggt ctccggtttc atcacgaccc
     9301 ggccccgggc caccgcctcc ctggccgtcg gttccttgcc ggagacatcg accatttttg
     9361 cccggccccg ctcatctaca tgcgtcagct tcaccatgtg aaaacgtcac ctcgccgcga
     9421 agtatcgcta cgcgtttatg aattccccat ccgccgggag ggtcggggta gaggggtgcc
     9481 agtttatccc gttctcccgt gatggctcct acgaacataa aacttatcac agcctctttt
     9541 tcccgtcaag acagttgaaa aatctctgga atttctatat atataccaca attttttctc
     9601 cgggggcgtt atgatcgtaa aagtcctggg cagctccgta atcggcattg agggttacct
     9661 cattgatgtc gaggtctata ccgccccggg gcttccccaa ttctccacgg tgggcctgcc
     9721 cgacgtggcc gtccgggaaa gccgggaacg gatcaaggcg gcggtcaaga gttccggcta
     9781 tcccttcccc cgtcatcatg tcaccgtcaa tctcgccccg gcggatatca ggaaagaggg
     9841 cacgggcttc gacctcccca ttgccgtggg catcctggcg gcggaagacg tggtccggcc
     9901 ggatctgctg ggcgcctatc tcctcctcgg ggagctgtcg ctcgacggcc ggatcaaggg
     9961 ggtccacggg gcgctgccgg ccgccctcaa gacccgggac ctgggcctcc ggggcatcct
    10021 ggtttccccg gagaacgcat cggaggccgc cctggtggcg ggtatcgagg caatacctgt
    10081 ggcttcccta actgaagtag tggaatttct gaacggaata agtgagataa aaccggcgcg
    10141 tcgggatgac cgggacatca tttccggagg tgaggacgag gattgtgatt tccgggacgt
    10201 ccagggacag gatcaggcca agcgcgccct ggagattgcc gcgtctgggg cccacaatat
    10261 cctgcttctc ggaccgccgg ggagcggcaa gaccatgctg gcccgacgcc tgtcctccat
    10321 cttgcccgcc ctttccttcg ccgaggccgt agaggcggcg aagatctatt ccgttgccgg
    10381 tcttttgaat cgcggcgagg taatgatccg ccgccgtccc ttccgcgccc cccatcatac
    10441 catctccgac gccggcctca ttggcggggg cgcgacgccc cggcccggcg agatcagcct
    10501 ggcccatcac ggggtgcttt tcctcgacga actgccggaa ttccggaaaa actccctgga
    10561 ggccctgcgg cagcccctgg aagaggggac ggttaccatc gcccgggcgg ccacgacggt
    10621 ggtctatccg gcccgttttt tgttcgtggc ggccatgaac ccctgcccct gcggatatta
    10681 tggcgacgcc cgccagacct gccggtgcag cgcccagcag atccgccagt accagcggcg
    10741 cctgtccggg ccgcttctgg accgcattga cctccacatc gaagtgccgt cgctgcgcta
    10801 ccgggagctg gtggggcgct accagggaga aagctccgcc gtaatccggg agcggatcga
    10861 aaaggcccgg gccatacagg agcggcgttt tgcgggaaag atgactccct tcaacgcccg
    10921 tatgtccgac gccgaggtca aacgctactg ccggatcgat gaggatgccc aacagttgct
    10981 ggagatggcc atggagaagc tcgggctgag cgcccgagcc tacaaacgga tcctcaaggt
    11041 ggcccggacg atcgccgatc tcgatgaccg ggagcttctg gaggcctctc acgtcgccga
    11101 ggcgattcag tacagaaatc ttgatcgaaa actcgtttag tgataatggt tgcgaccagt
    11161 catcaatcgg gagcagggta gggagtttct accgggcgtc gacctttcgg ccgttttttg
    11221 ccggctctcg aactatcaag gggggaaaag gaatattatg gaaatcatga ttgttcaggc
    11281 cgatccggcg aaaaggaagg ccaagccgaa ggatgagtcg aaactggggt tcggaagggt
    11341 cttgacggat cacatgttta ccatgtccta ccattccagc cggggctggt acgatcccgt
    11401 cgtccggccc tatgaggagc tccgtctcga tccggcggcc atgtgcctcc actacggcca
    11461 gctcatcttc gagggcatga aggcctaccg gggcaaaagc ggggagatat atctgttccg
    11521 gcccaccgag aatattcggc ggatgaacga atcggcccgg cgtctctgta tgccgaccct
    11581 ggatccggag ctttttttgg aaggcctgaa aaaactcgtt ctcctggagc aggactggat
    11641 ccccaaaagc agcggcgcct ccctgtatat ccggcccacc atgatcgcca ccgaggcnnn
    11701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncca aaagcagcgg cgcctccctg
    11821 tatatccggc ccaccatgat cgccaccgag gcagccctgg gggtccatcc cgccgacgcc
    11881 tatctgttct ttatcattgt cggtccggtg ggggcttact atcccgaggg attcagcccg
    11941 acaaagatct atgtggtgga ggaatacgtc cgttccgctc cggggggtat cggctattgc
    12001 aaggccgcgg gaaactacgc ggcgagcctc atggccagcg ccatggccat tgaaaagggc
    12061 tacacccagg tcctctggct cgatgccgtg catagaaaat tcgtggagga agtggggacg
    12121 agcaatatct tcttcgtcat cgacggcgag ttgatcaccc cgcctttggg agggacgatc
    12181 ctgcccggcg ttacccgcga ttcggtcctg cagatcgccc gttcctgggg cctcaatgtt
    12241 tccgagcggc ccctgtccat ggaagagata ctgaccgcct ttaacaacgg gtccctgcag
    12301 gaggcctttg cctcggggac ggcggccatc gtctctccca tcggacaggt ctgctaccag
    12361 aatcgggaat atgccatcaa tgagggccgt acgggaagcc tgacggagag gctctacgag
    12421 gagatcctcc ggattcagta cggagaaaag gaagatccct tcggctggcg tttgcggatc
    12481 gcctgaggtg acaaccgcgg cgccgcggat tagggacgct attccgggca cttgccccgg
    12541 caattcacaa agatgtggaa aaaactgttg ataactatgt gtacagtttt cctaagtcta
    12601 taaatgatac ggttttatat cggattgccg ctcttttagg cagacttaaa ggataaataa
    12661 aacaagtagt tacgcaggta tcgaagtttt ttcatgggtt aggcgtcccg ttgtgtgaga
    12721 tatgcacatg atccggaaaa tggcatgatt tttgaccggt gggtcatggc ggtcagggag
    12781 gtgcgaggat gtatagaaat ttgtttctca ttggggtcat aatagtggcg gccgccgttc
    12841 tgatcggggt ttccgggccg tccgcctggg gggagtcgcc cgccctggag gaggtgatcc
    12901 ggggattgca gaccgcctat gaaaaaacca ccgatctgaa ggcgtcgttc gtccaggagg
    12961 ttgccctggc gtcggtgaac cgcaaggagc gagaagaagg gaccgtgtat tacaaaccac
    13021 cccggcgcat gctctgggat tatacaaaac cagcggtaaa aaagctgatc ataaatcccg
    13081 acaaggccta tctgtatgtc cccaaggacc gggcggttta cgtacaggaa acggcccgga
    13141 tcttcaaatc ccagcttatt gtacgtctcc tcgacggtct cgggaaggtg accgaggatt
    13201 ttcaactcgc ctttgcggat cctggtgcgc ccacggataa aaacggcaac tacctcttgg
    13261 tcatgacacc gaaaaaggcc gatctcgggg tggacaagat cttcgtgacc ctgagtcacg
    13321 aggatttcac catcatccaa tgccgctttg ccgacacttt cggcaaccgg accacggtgc
    13381 tgttccgcaa cattcaacgc aatatcaagc tgcccgacaa gctcttccag ttcaagaccc
    13441 cgcccaaagt cgaggtctat ccgctttcat gaccgggcct cgctcttatc cgttatggga
    13501 ataatattgg ggatcaatca gggggaggat ggattcctcc cggcgcattc cgacgaagag
    13561 tggctcgagg tcaacggccg gggcggctat gcctccagca ccctgctgaa ctgtcatacc
    13621 cgcaagtatc atggtctcct cgttgctcaa ctggccgcgc tgccggggcg ttatgtcctc
    13681 ctgtccaaat tcgaggatac cataatccgc ggcggccgcc gggaagccct gagctgtcac
    13741 aagtaccccg gcgttttttt cccggcggaa tattcttttc tcaaggaatt tcagcaggat
    13801 atatgtcctt catttctctt ttccggcggc gggctccgga tcaggaaatc agtcctcctc
    13861 gtggcggacg aggacagtct cctgatccgt tacgatcccg aagcttgtcc ggcatctggg
    13921 gtgttgaggc tgcggccctt cgtggcctac agggattacc accacctgag caaagagaac
    13981 ccgttcctaa agggtaatgc cgacgcgatc aaaaacggtt tctccctcag gccttacgcg
    14041 ggaatgccgc ccctggtcat ccagacaaac ctgcggcccc gctggcaacc ggagccatta
    14101 tggtatcata acttcgaata tgccgaagaa gcggccaggg gatatgaaca tcacgaagat
    14161 ctcttcagtc ccgggctcct ggagataccg cttcggcagg gaaaggcggt cctggtcctt
    14221 gtatccctta cccctttcta cgggcggttg agcaagaaat gggatgatga ggtgcgccgc
    14281 cgccgggagg tccgagacaa ggatgaacgg actgtagcga tcctggcggc cggggggtcg
    14341 ctgacgggga gacaattccc ggggatggga gaggaatccg cggcgtccgg cggcgacgcc
    14401 ggtggagaaa cgaaaaccga gggcgatagg atgtctcccg gcaatgctgc cgccgggacc
    14461 gccgggaaaa gtcccgaacg ggaaatcgtc gcggcagtgg ccttggaaaa acgggacatc
    14521 gccatctcgg aaaccgactc tgccgggatc gccgcggagc gggaattact cggcatcctc
    14581 ctgcaggcgg gaaggcgttt cctgatccgc accgccgcgg ggcatccggc gatcattgcc
    14641 ggttatccct ggttcgggga atggggacgg gacaccctgc tgtccctgcc gggacttgcc
    14701 ttttgttccg gccgccgtcg ggaggggctg gagatactcg tccggatggc ccggtttgag
    14761 cgccagggtc tcctgcccaa cgtcttctcc gaacgggacg cagatcatgc ctacaatacc
    14821 gttgacgccc ccctgtggtt cttctgggcc gtccagcaac tccttctgga cgacgccggt
    14881 gaccggactc aggcggtggt ggaaaagaac ctctggccgg tgatgaaaaa catccttcgc
    14941 cagttcattc acggcaccat cttcaacatc tacatggacg acaacggtct gatccatgcc
    15001 ggcgcggaga accgggcatt gacctggatg gatgcctgca ttgacaaccg ccccgtgacg
    15061 ccccgccacg gttatcccgt ggaggttaac gccctgtggt acaatgccgt ccacttcgcc
    15121 gccgacctgg cctcccggtt cggggaccgg gagttctact ttgacgatct catcgagaaa
    15181 attcgccggt cttttctgga gatcttctgg aacgacgatg atgcttgcct gggggatgtt
    15241 taccagtccg ggtgcctcga tcgatccatc cgtcccaatc agattttcgc cgtctcgctg
    15301 ccccattccc cattggcgcc gacccaggcg cgcctggtgg tgaaaaaggt gcgggacgaa
    15361 ctcctcactc ccgtggggat tcgcaccctt tccccccggg atccgcgcta tcggggccgt
    15421 tacgccggct cgcccgccga gcgggacggg gcctatcacc agggtacggt ctggccctgg
    15481 ctttggggcc ccttcggaga ggcctggctc aaggtcgccg tcgaccggga aggggtaaag
    15541 aggacccttc gggatggcct ccgcaccttc ctgaccggcc atttccggat ggcggggatc
    15601 ggctgcgtct ccgaggtctt cgacggcgat tacccccacc ggcccggggg ctgtatcgcc
    15661 caggcctggt ccacggcaga gatcatccgt ctctacaccc tcatcggtgg atcgtagcaa
    15721 ttaccgttta ccgtgcgtaa ccgcggggca atgacatgac ggggaacggc agccgggcgg
    15781 gaggaaacag gttctgggga gaaacgacgg cctttctcgc cctgtttctc gacaatgtcg
    15841 gggtgctgat ctttctcagc gccatcctgg ttttcacttt caattatccc gcggagatcg
    15901 tcctgacccg catgatcccc ggtacggccg taggggtttt ctgcggcgac ctcatctata
    15961 cctggctggc cattcgcctg aggaagaaga ccgggcggaa tgatgtcacc gccatgcccc
    16021 tgggtctgga tacgccctcc accatcggaa tcgcctatgc cgtcctggga ccggcctatg
    16081 tggccacccg ggacgccgag ctaacctggc acatcggcat gaccaccctg ttcatgatcg
    16141 gcgccgtaaa gatcgtcacg tccttttgcg gcgcctggct gcagcgcgtc attcccaccg
    16201 ccggactcct gggccccctg gcggggatcg gtcttttgct tttgggtttt ctccccctcc
    16261 tggatgtctt cggcgagccc atcgtgggtc tcaccgccct ggggatcgtc tttgccgtta
    16321 tcctggcccg gctggagctt cccggccgga tgcccggggt cctcgtggcc gtcgttgtgg
    16381 ggaccctcat ccatctcgtc ctcggatacg ggggctatct gcctgaattc cggccgccgt
    16441 ccctggaaat gaagctctat atccccgttt tctcgttgga ttttatcaaa agtctgcccc
    16501 agagcctgcc ttatctgtcc atcgccattc ccttcgggat catgaccatc atcgggggca
    16561 tcgataatac agagagcgcc cggctggccg gggacgccta ccggagcagg gacatccttc
    16621 tcaccgaggc cttcacctcg ctcatcgccg ccttctttgg cggcgtggcc cagaccactc
    16681 cctacatcgg tcacccggcg tacaagaaga tggggggaac ctactggtac accctcttca
    16741 cgggggtgct catcggggtg ggggcggtgg tcggattgtt gtcgttgttc gtggcggtca
    16801 tcccccgggc ggccctggcc cccattttta tgttcatcgg cttcgagatg acgaggcagg
    16861 cttaccagga ggcacctgtg catcattccc cggcggttag catctgcttt ctccctgtgg
    16921 cggccaacat gatgcttatc atcctcggtc aatttctggg ccccctggcg gtcgcgccgg
    16981 acactctgcc gccccgcctc cagatcctcc atcagaatct gacggtattg agcaacggtt
    17041 tcatcgtcac cgggctgctc tgggggagca tgctggcctt tctcatcgac ggaaaaaagc
    17101 accgggcctc cctttgcgcc gtcatctgcg ccctcttcag cctttgcggc atcatccatt
    17161 ccgtgctgcc ttcgggggag atctatccgc cctggcggcc ggccggttat gcccattatt
    17221 tcattgcggc cggatatggg ttgctggcgg tcgttttcgc cgtcctccgg cccgggaagg
    17281 tcggggacga caagatattc aaggagtgac agcggtggtt tatgatatct ccggaggcgg
    17341 gaatcccgct gccggggagg aggcatgaag aaacgtgcag atgcccagca acggcgggtg
    17401 tgtggggtga tcaagagcgc tgggtgcggg aggaggaggt cgcaatgcca tactatgtaa
    17461 ccgacgatca tgtccgtctg tactacgagg tacggggaaa aggacggccg gtggtgctcg
    17521 tccacggtct gacggccaac cgccgtcact tccgcaaaca gacgccggag ctggccggag
    17581 aattccaggt cattaccatg gatctacgcg gtcacgggga ttcggaggtg ccggagcacg
    17641 gcctgaccct gccccgcctg gcccgggacc tcagggaact gacggaatat ctggaactca
    17701 cggacctttc catggtgggg tggtccctgg gtgcccatgt catcttcgag tatgtgaagc
    17761 agtattcctg ccgggggata gcgaaggtcg ccgtgatcga catggccccc cgactcctga
    17821 gggccccgga ctggccctgg ggtcttcccg gtgtcttcag caggaagacg ggggatttcg
    17881 gacccgagga caaccttgtc atgctgggag ccatgcttgg tgactgggag gcctacagcc
    17941 ggatcgtcgc ccagcggatc ctgaacaagt cccttttcaa cgaccggatg gaattcgacg
    18001 atcgggggga tttcaaaggc aaggcggatc ttccctggct gtatgccgag gcccggagga
    18061 acacccccca cgttatcatc gccctctgga tttctctgtc cctccaggat taccggccgg
    18121 ttttgaagga gatcacggcg ccgtgcctcc tggcctacgg tatggaaagc aactactatc
    18181 ccccggaaaa ttacgccttc atggcggagg cgatgccccg ggcccggacg gttccctttg
    18241 ccggctgcgg ccatgccctg cacatccagg atcccgaacg gttcaaccgg gaactgatcg
    18301 aattcctgcg ggaatagtga acacgacggt gtcgcctgcc ggagcaagcg ccggcagttc
    18361 attgtaccct ccagacggga tttgatcggt taatatgaat gcggatatca tggggatccg
    18421 ctgcggatga tcgatcctgt tcagttcata agtcctggca gaaagagggc aatttcggga
    18481 aacatgatca ggaggagaga gaggatgacc atggcactga gcaaatgcag cgccccctgg
    18541 aagatggtat gcagggggac gtcccgggcg acgccgccca cgacatagac gtttattccc
    18601 accggggggg tgatgacgcc aatctctgcg accaggacaa tgatgacccc aaaccatatg
    18661 ggatcgtaac ccagagtggt cactactgga tagaagacgg gaatggtgag catgatcatc
    18721 gccagggaat ccatcagaca gccgagaaaa agataaacac atatgatcat gccgataatt
    18781 gctgccggcg gcagggaaag cccggctacc caagtgccga cttccgtcgg gatggtagtc
    18841 actgccagaa aatgcccgaa aacggtagcc cccgcaacta tggtcataat catgcatgag
    18901 atccgcaagg tatcgttaag ggactgaaga aaggaccgcc aggccaggtt ccggcccaga
    18961 agggcaatga tcaacgttcc catggcgccg catcaggaga aagttatgct tgtccgcctt
    19021 ctggccttcg acctgggata cccgggccgc catgggtacg gcggaaacgt gggcggaacc
    19081 gatcaggggg ttgatcttct ctttcagaaa cagattcatg agcttggcca tgagcacccc
    19141 ccccgacggt actgaaggcg aaggcgacga ggccgaggat aaagattaag atcggctggg
    19201 gtttgagaaa ggcttcggcg ttcatcgtcg ctcccacgga cagtcccagg aatatggtga
    19261 cgatggacgc cgccggaaaa gcgaatcgca tttaccgatg gggaagagga tgggacagca
    19321 aagggaactt cacggcatga tgagtggcgc catgcttgaa aaaggaggag accggggaat
    19381 ggcgcttccc cggtccgaat aacacccggc cgctcaaaac cgggtatttt gatttcttaa
    19441 caacaaccct tgaattttct accggccgcc tcgaaaccgg cagccggtag aaatccttat
    19501 ttcaataaac caaaattact tcaccaacgg tttgggtacg aaatagatcg ctttcccata
    19561 gacggtgttg caggtaatgg ccccggccat gtaaacgccg atccagccca cgattatcag
    19621 caaccatccc gcgaagggct tcaggacctt cggatccccg aaccagccgg aatccatgcc
    19681 cacgatgcac cacagaacga tatcgaccaa gatgacgagg atgaacatgg ccgcgctgct
    19741 cttggcgtac gcgggggtaa cagccgtcag aaagcccgcg ccggccatcc agagccaacc
    19801 ttccacatgg acggccttgc cgaccatggc gcctacggcc ttgccgatgc cggccttggc
    19861 ggccgcgagc tgctccggcg taagcatctt gacatccgta accccggccg cggccatggc
    19921 ggcgtccatg gactccttgg ccgccttggg aatgaagatg aaactgatgg aataccattt
    19981 ggcgaagacg ctcagggccg ccgcaaacat gaagaatgcc gagaagaaca aaaagacgtt
    20041 gccgccggtg atgttgtggt ctttcaattc catgaccgcc gtcgtgtact ggacgatgcc
    20101 gccgccgatg agccaggccg acagcaaggg gaaaccggag acatccactt taccaaggaa
    20161 aaccgcccca aaaccgaaac aggctacggc cagcgcggcg agaccgccgg gacccggaga
    20221 tgcaaaacta tgttcgtgtg ccataaaaac cctccttatt tcttcgttac gataagaaaa
    20281 aaaataaaat gaagcgatca aactaaccta agcatgccat ttgccgcttt tttctccttt
    20341 catcccccct ttccccacaa ccgtcttctc ttgtccgcaa accgcgagat ttgccttacc
    20401 gtcaaaaagt tgttgctaaa ttatggataa aaaattattc gctgttccga catggaagct
    20461 attaagaatt gaaatcgaac acatccgccc gcccttccgg aggcgaatga agatcaccgc
    20521 accgccgtca aatcaggccg caatctcccc ggtcgcgggg cttgtcgatg tgctcttttc
    20581 ctcatctttt ctatatctaa aagtgtgcca gatatttatc ctcatgaaat cgactacttg
    20641 tcattaagag gaagaaataa tcacgatatt tggcgaaatc tgttgtgaaa tatggtgatc
    20701 tccgttatat atcgcccggt cgcggtggcc aaatcttgga aaaggggaaa aaatcgacat
    20761 ggaagcgtca ggaaacgagt ttttccggca ggccaccatc cggctctgca gcagtctgga
    20821 aatagaaaag gagatgttca actgtctctc ctatctgcgg aattatctgc ccgcccggga
    20881 gatgctgctg atcatcttcg atcccatccc ggggttgatt cacaatctgg cccgggtgag
    20941 tctggaaggg atcggtaaac cctttcgctc caaatccctt tcggaggagg cgatctgccg
    21001 gatcaactat gacactgcca cctggcagga ggtcaagatt ctcgatcgcc tggaattgca
    21061 ccccgccggt tggatcctct atgaccatgt aaatctgtcc atgacctcca tgatgtacat
    21121 cagtctggtg attgaaggga aaatattggg gtcgatggtc tttctggcag acggcagggg
    21181 gatgttcaaa agggagcatg ccgacctcat ctcccaattg cgggaacctt tcaccatcgc
    21241 cgtctccaac gccctgcgct accgggagtt gaccaagctg aaggatatcc tggacgcgga
    21301 aaatcaggac ctcacccggg agctgagata tgttacggaa gatgatattg tcggcgccga
    21361 tttcggcctg aggcaggtga tggagatggt cagacaggtg gcctccctca agagccccgt
    21421 gttgctcctg ggagagaccg gcgtgggaaa ggaggtaatc gccaacgcca ttcattacgc
    21481 ctccccgcgg agggagcggc cgctggtcaa ggtgaactgc ggcgccattc ccgagagcct
    21541 tatggacagc gaactcttcg gtcatgagcg gggggccttt accggcgcca tcgcccagaa
    21601 aaagggtcgc tttgaacggg ccgacaaggg cagtatcttc ctcgacgagg ttgccgagct
    21661 gccgccgtcc attcaggtca ggctgctcag ggtgctgcag aacggagaaa tcgagcgggt
    21721 gggaggggcg gaaaccatcc cggtggatat ccgcgtcatc gccgccaccc atcgcaacct
    21781 ggaggagatg gtgcgtaacg gcaatttccg ggaggatctt tggttccgcc tcaatatctt
    21841 tcccatcatg attccccccc tgcgaaaccg ccagggagac atcccgaacc tgatccgcca
    21901 cttcatggag aagaaggccc gggagcttaa gattcacacc ccgccgccca tgtccgccca
    21961 ggtcctggaa aggctcaagg aatatcactg gccgggcaat gtccgggagt tggaaaatct
    22021 cgttgaacgg gaattgatca aaagcagggg taccaacggc aaccgtttca tgaccttcga
    22081 gcatttccgg tggccccggc gttccggtcc ggcctgggga gctccggagg acaacgggaa
    22141 tttcctcacc atggacgagg cgatgaccca tcatatccaa ctggccctgg aacagaccaa
    22201 gggcaagatc tacgggtccg acggcgccgc cgctttgctg ggaatcaacc ccaacaccct
    22261 gcgcagccgg atgaagaaac tggggatctt tccgcccgga tcccagaatc ggcgccggct
    22321 tcgggaagga gcggtgcgag gttaaagata ttgacagccg gcggaaaata accgaaaatg
    22381 atcttaccct ggagatgcag ggttggatag ccggatagaa tcaacctcct gttcgtcatg
    22441 aaactgattt atgccaccga cgttcatggc tccttcgagc gggtgcggga gctgctccgt
    22501 cttaccacgg ccgatgttta catcattgcg ggtgatctgg tcgatatacc gttctatacc
    22561 gtggagaagg cgaggcgtta ccatgacctc cagacatatt tccacagtct gaggcgggac
    22621 cggggaaaga cggccgccac cctcgaagat ttcgtcgcaa ccctcctgga ggagcccgat
    22681 ctgcccggcg aagtgtggga gaaaggggac cggtttcgct acgagaccat gcgggcccga
    22741 catatcctcc agaagcacta ccggcttctg gacaacatcc tctctttggc tacggcgggg
    22801 acagccttcg ccctgccggg caatcacgac atggatctcc accatacgct gttgcggggc
    22861 agggacctcc atctccagtg tcacgagtca aaggggataa aaatcgccgg gtacggggga
    22921 gccgcgggaa ataccccggg cattccggag cgtttcggag tggcctatcg agaaggcggg
    22981 ggtggcaaga acgatgaaat gtaccgtttt tttcaatccg tgcggcccga tgtcatcgcc
    23041 gtccatcagc ctccttacgg aattcacgat tttgccccgc ccatgggtga aactggttcc
    23101 catgccctgc gtcggtattg cgaacgacac cccgtgctcc tctgcctcac ggggcacctc
    23161 cacgaccagt ggggactgga ggaggtggag gacacggtgt ttctgaaccc ctcccatttc
    23221 ggggaaatcg ttcaggcgaa cggaacggtt gcggaagggg gcttcttcca tgaattggaa
    23281 attgacggcg gaaagatcgg cggtgtccgg caccggaaac tggtggggga tctgatccac
    23341 gacctggtgt attatgaacg ccgggaagga gaatgggccc ggcgggtggt ggaccgggaa
    23401 cgttacgggg cgctgctgag ggggatcaat tgcgagccgc tatgaggcgt ttagcgccgg
    23461 cggtcggaaa gggcgggaga catgacgagg aaaggcgaag cggatgtaag gttgctgggg
    23521 aggatggcga atctggctat ttcctgcctt accggggaag aggtcgtcgc cgttgcggcc
    23581 cgtttcctgc cgcagctttt tcccggggaa tccggggcgc tttttctccg tcccgccgcc
    23641 gggaaggagt atgaaccggc ggcgtcctgg ggcgaggaga gggcatggcg tcccttttcc
    23701 ggcgccgact gctggtccct gcgccgccgc cgtctccata gcgtccgcca gggaaaggac
    23761 cagcccctct gcccccacgc cgaccacgat ccggagcggt ggatttccct ctgcatcccc
    23821 atgacggccc gggaagatgt cctgggtgtc ttccacgtcc tgatcccggt ggaagagagc
    23881 tcgacagatg ctgggaaggg gccgggggac cggctgcttc aaggtggttc tccaatcccg
    23941 gccgcgggtt cggaaaccgg agaagccggg gaactcggcg gatcggatac ctacctgggt
    24001 gaaggggcgg acgaaaggag gctcgccgcc ctgacggcgg acatcctgac gatgattttc
    24061 gtcaatctcc ggctccggcg gcagatcgac gacctcgccg tacgggacgc cctgaccgga
    24121 tgcttcaacc gccgctatct ggaggacagc ctggccaggg aactcaaggt cgtccagcgg
    24181 accggccgtc ccctggcggt ggttctcatg gatatcgacc atttcgggcg gttcaccgac
    24241 gccttcggtc atgggtcgag cagcctggtc ctccgggagt tgggggagtt tctccggaat
    24301 aacgtccggg atgtggacct gagctgccgc tacggcgccg atgaattcgc ccttctgttt
    24361 cccgatacgg atccgccggg ggcccggcgc cgggcggagc atctccaggc tatgctccgg
    24421 gatctctccc gtcatggcgg gggctcccat ctgcgccgga tcaccctgtc catgggcgtt
    24481 gcggcctacc ccgatcaggg ggaaacgtgg ggagatctca tggaggcggt gaaggccgcc
    24541 ctgaaaaggg ccaagcagac cggtcgggac cgggtctgcg gcggcgagta gaggacaagc
    24601 cgccagcccg cgatgattta cttttttccg acaatctgcc atatataacc tctgtgaagg
    24661 gagggatgat gggcatgcgg gtcctcatgt tcgggtggga gtttcccccc ctggtcagcg
    24721 gcggcctggg cacggcctgc catggaatca cccgggcgct tgtccggctc ggccaccagg
    24781 tgatttttgt catgccccac ggcgaaggtg acgcggcctt tccggggttg acgatcgttt
    24841 cggcatccgc ggtcctcggt gcgttggccg cagcccgtac ttttccgtcg ccggtggacg
    24901 atgcgcccga ccttttacct gcccgtctcg ttctccatcc ttacctgacg gttcgtcccc
    24961 cgaaagatgg cgttccggcg ttccggaagt ggccggccga cagtatactg gcaggttccg
    25021 gcggttacgg ggccgatctt ttcactgagg tcggccgcta tggcgaggcg gggaggatcg
    25081 tggccgcccg gacgagcttc gacgtcattc acggccacga ctggatggcc gttcccgcct
    25141 gtctggcggc ccgccgcgtc tccgggcgac cttttgtcct ccatgtacat tccctcgaat
    25201 atgaccggag cggcgcgggg ggcaacggcg aaatctgcgc cgtggaacgc cggggattgg
    25261 aggcggccga ccgggtcatt gcggtcagta attacacgaa ggagatgatc gtccggcact
    25321 acgggatcgc ccgggagaaa atcgccgtgg tgcataacgc cgtgacggcg gcggagggga
    25381 gaaaagccta tcgcgtcccc gccggaaacg gagagaagcg ggttcttttt ctggggcgca
    25441 tcaccttcca gaaggggccg gagtattttg tcgaggccgc cgcccgggtc ctcaaggaga
    25501 tgccggagac caccttcatc atggccggat ccggggatat gatgccggag atggtggaaa
    25561 aggtcgccga actgaagatc ggcaggaggt ttcactttac cggcttcctc cagggccggc
    25621 aggtggagga ggcctttgcc ggggcggatc tctatgtgat gcccagtgtc tccgagccct
    25681 tcgggcttac ccccctggag gcgatgatgt acgacgttcc cgttatcgtt tccaggcagt
    25741 ccggggtagc cgaggtcctg aaacacgccg tgaaagtgga tttctgggat gtggcggccc
    25801 tggccgaaaa gatgatcgcc atcctcaaat atccgccgct ggctgcggag gtggtcgctc
    25861 gaggccggga ggaactccag gggctgagct gggaagccgc ggcccggaag atcgttgccg
    25921 tgtacaagtc gttatgatca gtcgcccgtg tcccgactcc catggccatc tcgattccca
    25981 cccggagaag gaggcggata ttcgtgagtg aatacggaaa taaaaatcgg catacccgtc
    26041 tgagggagcg aacgattcac ccggcgggca gcgggtggcg gttgacgccg gggcggcgtg
    26101 gcgggccgat acacggcgcg ccaccggcgg ggatgagcgc atgaccgccg tttgcctttg
    26161 ctttcaggtc caccagcccc gccgcctgcg gcgctacccg gtgttcgcga tcgggcggga
    26221 gcggaattac gaagatgagg ccggcaacag gaagattttg gacaaagtcg tcgataagtg
    26281 ctatcttccc gccaatgacc tccttttgag tctgatcgat cggcacggag ggaaattccg
    26341 tctgtccttc tccataacgg gggtgcttct cgatgaactg gagcgttttc atccggaggt
    26401 cattgccagc tttcaagcgc ttgccgggac gggatgcgtg gagtttctca acgagaccta
    26461 tcatcattcc ctggcctttt tatatagcga cgaggaattc cgcggccagg tcgaccggca
    26521 ccgacggcgg attacggagc tcttcggcca ggaaggagag acctttcgca ccacggagct
    26581 gatctacaac gacgccctgg cggccgcggc ggcggaaatg gagtaccggg cgatccttgc
    26641 cgaaggggcg gcggccgtcc tgggaccgag ggatccgaac cgggtttacg aggcggccgg
    26701 cgggggggga atcagggtgt tgccgcgcaa ttaccggctt tccgacgaca tttccttccg
    26761 cttttcccgg cgcggctggg aaggctatcc cctgaccgcg gcgaagtacg cccgatggct
    26821 ccaccggctg cacggtccgg agaccgtgat caacctgttt atggattatg agacctgggg
    26881 tgagcatcaa tggcgggaga cagggatctt ctcctttctg gaagctctgc cgggggcaat
    26941 cctcgccggg gcggattttg tcttcgtaac gccccgggag gcggcggccc ggaagccggc
    27001 gggagtgctg tccgtgccgg ggttctgttc ctgggccgac acggaacggg accttacggc
    27061 ttggcggggc aatgccatgc agcgggacgc cctggaggcc ctttacgggc tggaggaggc
    27121 ggtgacggca aagggggacc gggacctggc ggagcagtgg cggcagctcc agacctcaga
    27181 ccatttctat tatatgtgta cgaagtggtg cgctgacggg gatgtgcaca ggtatttcaa
    27241 cccctatccg tccccctatg acgcctacat caactacatg aatatcctgg acgatttttc
    27301 caggagggtt ggaaaggaaa agggatcatg acggaagtcc gcaacggtta tctgttcgag
    27361 gtgagctggg aggtctgcaa caaggtgggc ggcatctaca ccgtaatcgc cagcaagctg
    27421 cgggaggcgt tgaaggtcta tggggaccgc tatatccttc tgggacccga tttgaagacc
    27481 aatgtggatt tcgaggagac cgacgaggac tgctgggcga agatacggga agggacggcg
    27541 atcaaggata tcccctgtcg cttcggccgc tggaaaatat ccggggagcc caaggcgatt
    27601 ctggtgagcg ccggcaagaa atacgacaaa gaccagcttc tgtttcgcct ctgggaggcc
    27661 ttcggcgtcg attccatcgc cggcggctgg gattacgtgg aacccgtcat gttcagcttc
    27721 gcctgcgggg aggtgatcga gacaatctac aaccttgccg tccgccccta caatcttgcc
    27781 gccgtcgctc actttcacga gtggatgacc ggtgccggtc tgctctatct gaaaaagcgc
    27841 gtacccgagg tgggaacggt gtttacgacc catgccacga tcctcggccg ttcccttgcc
    27901 ggtgccgggg tggacatcta cgggatgatg gaacatctcg ccccccagcg ggaggcgagt
    27961 gcccacaaca ttaccgccaa atattccatg gaagcagtca cggcccggga ggccgactgc
    28021 ttcaccacgg tgagcgagat taccgccgcc gaggcgaaaa atttcctctc ccggaccccc
    28081 gatgtgatca cccccaatgc cctggacatg gagaacattc ccgactacgc cctttcccgg
    28141 gcgcccgcgg aacgttcccg ggagaaactc cttgccgcgg cggcccggtt tctccgccgg
    28201 gagctcccca gggaaacaag gatcctcatc atttccgggc gctacgagta tcacaacaag
    28261 gggatcgaca tcttcctcga ggcgttggga cgtctggaga aggagggccg cggtggtccc
    28321 ggcgtgctgg ccctcctgtt cgtcctgggg ggctatacgg acctgattcc tgccctgcgg
    28381 gacgatacgg gacgctctga aacgggcaag tcgcccctcg tgacccaccg ccttcacaat
    28441 gaaggagcgg atcccatcct gcaggcctgc aaccgtctgg ggttgacgaa ccagccgggg
    28501 ggaaaggtac aggtcatctt caatcccgct tacctaaacg gtcatgacgg cctcatcaac
    28561 atgtcctatt atgaagccct gtccggttgc gacctgggag tgttcccttc ctactatgag
    28621 ccctggggct acacccccct ggaaagcgtc gcctatgggg ttcccaccgt caccacggac
    28681 caggcgggtt tcggcctctg gtgcgaacgg acctgtgagg agaacggagg ggtgatgctg
    28741 ttgaggcggc gcggccgtcc gcgcccgtcc atcgaggatc atctcttccg gatcgtcggc
    28801 gattttctgg actggacgcc cgaggagatg gcggcccacc gccggagcgc ccgcgaggtt
    28861 gccctgcggg ccaattggcg cgattttttt gattattatt tgcaggctta cgaccgggcg
    28921 ggaagccgcg ccctaaaccg ggcggaacag gcggccgcaa aggcggagcg ggcggaagtc
    28981 cggcatgttt atgccggcgc cgtttccgcc cagccccact tccgttcctt caccgccgtg
    29041 gcgaatctcc ctgagcagat catccgcctc cgggagctgg cctacaatct ctggtggacc
    29101 tggaacccac gggctttgga tctttttgcc accctcgatt tgaagctctg ggcggagatg
    29161 ggcaacaacc ccgtgcgcat gctggaaacg gtttccccgg acatcctgcg ggaagcggcg
    29221 gccaacgcca gctacctgaa cctgtataat cggatcatgc agcagttcga cgaatacctg
    29281 ggggagaagg gaccccggcg gcgcctgccc gcgacggcgg gtctccgctg gtcatccccg
    29341 gtggcctatt tttccgccga atacgggctc cacgaatgcc tccccatcta ttcgggggga
    29401 ctggggaccc tctcgggaga ccatctcaag acggcgagcg atctaaatct gcctctggtg
    29461 gccgtggggc tcctctacaa gaacggttat ttccgtcaga tgatcgacga acagggccgc
    29521 cagacggagg catacccgga aaacgatttc tccagcatgc ccgtccggat cgtccaggac
    29581 gacaagggga atgaggtgca gatcgccatc gatctgcccg gacggaccct attcgccaat
    29641 atctgggagg taaaggtggg caaggtgtcc ctctatctcc tggacacaga tgtgccgcgt
    29701 aacacccagc aggaccgggc gatcaccgcc cggctataca gcgccgatca aaagatgcgg
    29761 atcgagcagg agatcctcct cggggtggga ggggtgcggc tcctcaaaaa gctgggtatc
    29821 cggccccggg tttatcatat caacgagggg cattcggcct ttctcatctt cgaacggatc
    29881 gaaaccctca tgaaagaaga aggtttgacc ttcgaagagg cggcggaggt ggtgcgggcc
    29941 agcagcgtct ttacgaccca cacccccgtg gaagcgggca acgaacgttt ttcccgggag
    30001 atcatggagc attacttctc cggcttcatc aaacgttcgg ggctctcgtg ggtgcagttc
    30061 tgggaactgg gacgccggga cggcggggag gagaaaaact tcttcatgaa tatcctcgcc
    30121 ttcaagatga ccatccgcag caatgccgtc agcctggtcc acggccaggt ctcccggcgc
    30181 atgtggcggg atgtctggaa aggattcgac gatacggaca tacccatccg ccacataacc
    30241 aacggcgtcc atgtcctttc ctatctcgcc ccccggatgc ggaatctcct cgacgcctac
    30301 ctgggcatgg actgggaaaa gatcctaacc gacgccgagc ggtggagccg catccaggac
    30361 atccccgacg ccctcctctg gcgggtgcgc tatgaactga aacaaaagac catcgacctg
    30421 atccgggaag acatctccaa agggggagcc aagtatggca cggcccatgt ctggcgggaa
    30481 gaactctttt cccgattgaa tcccggagcg ctgatgatcg gcattgcccg gcgtttcgcc
    30541 ccttacaagc gggcggacat gatcctcgcg gacctggatc gtctggaccg gatcgttaac
    30601 catccccacc ggcccgtcca tctggtgttt gccggcaagg cccaccccaa cgacggcgtc
    30661 ggcaagcatc tcgtggaaaa ggtcgtgggg atctgccggg acgatcgctt ccggggacgg
    30721 atcttcttcc tggagaatta cgacatccgg gtcgcccgcc atctggtcca gggcgtcgat
    30781 ctgtggctca acaacccccg tcgcccactt gaggccagcg ggaccagcgg gatgaaggtg
    30841 gtgatcaacg gtgtcctcaa tatgagcgtt tccgacggct ggtgggtgga gggatacaac
    30901 ggcgccaacg gctggacgat cggacccgtg ctgaaaggct atgaggaccc ccccgccgat
    30961 gccgatgcgg aggacggcca gtccctgtac aatatcctgg aaaacatggt gataccggca
    31021 ttttacgacc gagatgccgc gggtgttccg gtcaaatggc tggcgacggt caagcagtcc
    31081 atgcaatctc tggcccccca attcaatacc gaccggatgc tccaggagta tctccggaac
    31141 atgtacggtc cggcggcccg acgggagtgc gaactcgccg ccgatggata cgacctggct
    31201 cggcggctgg ccgattggaa agggaagctc cccatgcgtt tttcctccct caagctcatt
    31261 gatgttagcg ttgaggggat tcatggcgat acggtcatgg tggatcagcc cctgaagatc
    31321 agcgcccgga tcgatcccgg caagctcgcg ccggaagaga tcctggtgga gatcatgatc
    31381 ggccgccgga atggccatga ttttgtccgg ccgccggaat gcgtccccct ggaaattgcc
    31441 ggaagatctc cggaagggat cctcaccttc tccacggagt atttcgttcg ggagaacggc
    31501 ctgtacaatt acggtgtccg ggttatcccc cggcacgagc acctggcctc gaaactggag
    31561 acggggctga ttctctgggg atagccgcga ctcggtgaga tggcgggtga agttgcctcg
    31621 tatggacttt tttgtgctgt cgtcattatg gttctttgat cttttcgggg ctgctggcgg
    31681 agtgaggcgg acgacggcga gatcttggaa agggggttag caaggcatga gcaaaggggt
    31741 gtttatcgcc tccgtcgagc cgaggagtgg caagtccctg ttcgttttag ggatgatgaa
    31801 cgccatcgtg aaccaacggg ggtgtgtcgg ctatttcaag cccgtcatcg aaaccgatgg
    31861 ccggggcgca aaggacgcgc atgtcgagat gattcggaag catttcgatc tgcccttcga
    31921 gtacgaggcc cttttcgggt tccggcgcca tgagatcgcg gccctcctca cccgcgggga
    31981 agattaccat gtcgtggcca ccctgctgga caagtacaag cagatcgagg acgcctgcga
    32041 ttatactatt gtggaaggaa ctgattttct tggtaagaac accttttttg aatttgacgc
    32101 caacgtggcc ctcccgaaaa atctgggcat tccggcgatc atcgtcgttt ccggggagga
    32161 caagacgccg gatgagataa agggaggcgt ccttggcgcc tatgggggat accgggaagg
    32221 ggacgtggag gtcctggccg tggcggtcaa caaggtgcat tcagacgccg ttcccctgat
    32281 cgagcccctg ttgcggccca tgctgtcggc ggaaacggtg ctggcggtta tcccggagaa
    32341 tcccaagttg aagaatccca cctttcgcga ggtgagcgcc gccctgggcg cccgaaccct
    32401 cttcggccgc catcgtcatg agaaccgggt cgatcatacc gtcgtcgggg cgatgctcat
    32461 ctccaatttc ctgaatcatc ttcaggacaa caccctcatc gtcaccccgg gggaccgggc
    32521 ggacatcatc gtcggggccc tccaggccaa catctcccgg aactatcctc gcctggccgg
    32581 gatcatcctt accggtgctc tggacatgga ccccgccatc gtgaagctca tcgaaggcct
    32641 gcaaaccgtg gcgcccatca gcgccgtaac cacggggacc tacgaaacgg ccaaccgggt
    32701 cggagcggtg cattcccgga tctatgccgg caatgcccgg aagatcgctc tggccatgga
    32761 tgagtttgac cggtacatgg acatggagaa actgggggaa aagatggcga ccttcaagaa
    32821 ggcaggcgtc accccctaca tgttcaagta cgggatcgtg aagcgcgcca agggcaggcg
    32881 gcggcatatc gtcctgccgg agggggatga cgaccgtatt ctcactgccg cgtcccgcct
    32941 cgtcgaacag gaggtggtgg acctgaccat ccttggtgtc agggaaaacg tcctcacggc
    33001 gggaaagcgc ctcggcgtca atctggaatg ggaggaaatc cgcattgtcg atcccgtccg
    33061 gtcggaatat tatcaggatt acgccgccac cctccaggag ctgcgtaagg agaagaacgt
    33121 taccatcgag atggcccggg acctcatgac cgacgtgtcc tacttcggca cgatgatggt
    33181 ttacaaagga cacgccgacg gcatggtttc cggggccggc catacgaccc agcacaccat
    33241 ccggccggcc ctgcaattcg tgaagaccag accgggtgtt tccgtggttt cttcggtctt
    33301 cttcatgtgc cttcccgacc gggtgtccgt cttcggagac tgcgcggtga atcccgatcc
    33361 cacggcggaa gagcttgcgg agatcgccat ctgctcggcg gaaagcgcgg cgatgttcgg
    33421 catcgaaccg cggatcgcca tgctctccta ctcctccggg acctccgggc agggggcgga
    33481 cgtggagaag gtccgccggg ccacggagat cgtccgggcg cggcggccgg agctgaagat
    33541 cgccggcccc atccagtacg acgccgccgt ggatcccgcc gtgggcaagc agaagatgcc
    33601 cgattccgac gtggcgggcc aggccagcgt cctgatcttt cccgatctga acacggggaa
    33661 caacacctac aaggccgtcc agcgggaaac cggcgccgtg gccatcggcc ccatcctcca
    33721 gggtctgaac aagcccgtca acgacctgag ccggggctgc acggtggagg acatcttcaa
    33781 caccgtggtg atcaccgcca tccagagcca ggaggggatg acggattgac tccgcccgct
    33841 ggcgactcgt caggggcgct tcccgatcct gcccctgccg atttgtcggg agtgtttacg
    33901 gagcttgtcc cggcccgctt tgtcaggagc gcttccggat atttcccgcg gcggcgatca
    33961 ccaccacttc ttcgggaatg tcccggccgg cggcggccat tcctttttta acgatcatgg
    34021 gcaggcccga acagcacgga acctccatta cggccatggt gacgctccgg atgtccgcgg
    34081 tgcggaagat gtcggcgaac ttctccgtat aaccctggac gtcatcgaat ttcggacacc
    34141 ccatcagcac cacctttccc ttcatcagat cgcggtggag ggtcggcatg gccacggcgg
    34201 tgcagtccgc aaggaccagg agatcggccc ctttcaggaa gggggcgtgc ggagggacga
    34261 gccggatctt taccggccag tgggtgaggg acgactcccc gcgccacgcc cgtggctccc
    34321 cgtcccctcc gccttcctcc ttccgccccg ggccttgtcc cgcggagatc tcccggggcc
    34381 ggtttgcctc cgtgcaggag acgccaggcc cgaggatctg gataaaggtg gaggggcagc
    34441 cgcagggcag tgtgtcggcg gccccggcct ccggatgacg ccgcgcgacg gcctcttcat
    34501 cgaaaggcgc cgcttcccgc tcgatgaggc gcaacgcccc ggtggggcag tctccgatac
    34561 aggcccccag gccgtcgcag tagctctccg ccacgacccg ggccttcccg tcgatgatct
    34621 gcagcgcccc ctccgcgcat ccggggacac agttcccgca tccgtcgcag agctcctcgt
    34681 cgatttggat tatcttgcgc aaaatcttcg tcacgtcacc gctcctccct tgtttttttc
    34741 ggtctgtcct ctttccggcc cgatataaag caggcgcacc agcgggggaa aagatggatt
    34801 catgtaccgt aaagggtcat atagcagata aaacaagtat gaccttgact tatgtcaaga
    34861 cccggatata tttttgcgcc agggatgaga aaatggaaga tgcacgtaag gcattgtcag
    34921 caagccagct tttcggaggg ctgccggaag cggacctcgc agcgattgaa aagatagcgg
    34981 cagtcaaaca cttcgacaaa ggggagatca tcttctttga aggagacgag ggggtcggtt
    35041 tctatctcgt tgccctgggc cgcgtgagcg tgttcaagct cgcgcccgac ggccgggagc
    35101 agaccctcca tctcgtgaag gagggggacg ctatcggcgc cgtgcccgtc ttctccggaa
    35161 aatcctttcc cgccaacgcc cgggcgctca cacaatgccg gctcatcttt atcccccggg
    35221 agaagttcat acaactgatc gcggacaagc cgttgctcac gatgaatctt cttgccctcc
    35281 tggccacgcg gctccgggag ttcaccatcc aggtggagaa tctctccctg aaggaaatcc
    35341 cgagccgcct ggcggcctat ctgctgtatc tgtcccggga acaggggggg agcgatctga
    35401 tcggcctgag cgttaccaaa gggcagctcg caagcctcct gggggcgggg ccggaatccc
    35461 tgtcccgggc cctgggcagc ctgaaggcca ggggactggt ggcggaggat ggggagggga
    35521 tccggctgat caatcgcacc ttgctgacgg agcttgcgga aagcggccgg gacttccggt
    35581 aaaaccgccg ggcggcgggc gcagtacgag ttacgccttg gcaacgccct gatccactat
    35641 cccatttgcc ggcaggaact ctcgtaagcg aatatcttct gattcggcac tgaagcaaat
    35701 agaggacttg ctacgagccc tgaaagaccg cggacaggat ttacttgaag aattctttgt
    35761 tgaggagagg tgaaatggcc aaggataata acaacggcgc gaatctcggc ttcgaatccg
    35821 acctctggcg cgccgccgac gtcctccgct ccaacatgga cgcggcggag tacaagcatg
    35881 tcgtgctcgg tctgatcttt ctcaaatata tctcagacgc attcgaagag cagcacgcca
    35941 ggctcgaagc ggacagcgcc cagggcgcgg atcccgaaga tcccgacgag taccgggccg
    36001 tcaacatctt ctgggtgccc aaagaggcgc gctggtccag gctcaaatcc gaagccaggc
    36061 agccgaccat cggcaaggtc atcgacgacg ccatgctcgc catcgagcgc gacaacccgt
    36121 cgctcaaggg cgtgctgccc aaggactacg cccacccgcg cctcgacaag cagcggctcg
    36181 gagctcatcg acatggtggg caacatcggc ctgggagaca aggtcaaccg ctccaaagat
    36241 attctcggcc gggtctatga gtacttcctc tcccagttcg ccagcgctga gggcaagaag
    36301 ggcggtcagt tctatacgcc ccgctgcgtg gtccgggtgc tggtcgagat gctggcccct
    36361 tacaagggcc gcgtctatga cccctgttgc ggctccggcg gcatgttcgt ccagtcggtg
    36421 gaattcgtga aggcccacgc caccggcaat gggaatggag gaaaaacaaa ggctgatgtc
    36481 agcatcttcg gccaggaatc gaaccacacc acctggcgac tggccaagat gaatctcgcc
    36541 atccggcatc acaaccacat cctcacgccc ggtcgctatg tcggcgcagc cgaggtcgaa
    36601 gacgacggcg aacccttcga agagaagatg gccagactga cgaccgcctt gcgggagcag
    36661 actgggaagg ctgaaaaact agaccggatc atctgggcca atctggagga cgtcggctat
    36721 gggggataaa aaaccggcga aagccgacgg ctttgcggtg cttctttctg aagtgaaaca
    36781 gcgcatccag cttgcccaga cccgggccgt tctggccgtc aactcggagt tggtccgcct
    36841 gtattgggac atcggtcgta tcattgattt gcgtcagaag cgcgaaggct ggggtgcggc
    36901 ggtcattccg cgtcttgcac aggaactgaa aaacgagatg cctgagctga agggtttttc
    36961 ggagcgcaat atcggacgga tgattgcttt ttatcgggag tatgctgatc cgtctgcatt
    37021 tttgccgcag gctgcggcaa aaataagttc cccggcaaaa gtgccgcaac ctgcggcaaa
    37081 atcgggaaac gtgcttctct ggtcaatccc ctggtttcat cacgttgttc tgatggaaaa
    37141 ggtcaaagac attccaacgc gatactggta catggagcag gcccttacca acggctggag
    37201 ccggaacgtt ctgaccctgc aaatcgaggc acaggctcat gcccggcacg gcaaggcccg
    37261 gtccaatttc gccctgacgc tgccgccgca tcagtccgat ctggtgcaac agaccctcaa
    37321 agatccctat attttcgatt tcctgacgtt gacggaacct tttcacgaac gtgaactgga
    37381 aaccgaactg gtgcgccatc tggaaaagtt tttgctggaa ctgggccagg ggtttgcctt
    37441 tgtcggaagg caatacaagc tcgatgtcgg agatgaggag ttctacatcg atctgctctt
    37501 ctaccacctg cgcctgcgcg cattcgtggt catcgaactt aagaagggca aattcaagcc
    37561 ggaatatgca ggcaagctca acttttactg caatgtcgtc aatgaccgcc tcaaacagcc
    37621 ggccgaccag cccaccatcg gcctgatgct ctgccaggag cgcagccgcc tgctggccga
    37681 gtatagtttc gtcgggatag acaaacccat cggcatttcc acctacaaac tcacccgagc
    37741 cttgcccagg gaactgcgtt ccgcgctccc caccgtcgag gagatcgagg cggaactggc
    37801 agaagtggtg aagcaaaagc caaagggaac tggcggacgg aaaaagaaag gcggtgggga
    37861 atgattgtct tgattgtgaa aaacgtgaag gacaagaagc ccgatcagat tatccctgca
    37921 tgcgccaagc aaggcgaggc aggcaggtgg gccaatctgg cctgcctgtg cgtgccagca
    37981 cgcagacggg aggacatcgg ctatggagga tagtggaatg ttttacggtc acagtttgtg
    38041 acctcaaaca tggatatgga actttcaata aatccctgcc ttgaaatcgc aattggcgac
    38101 atcaagtgca tcctggataa tgggttggct tgggaattaa agcagtaaat caaccgtctg
    38161 ttaaagcttg ggggaaggcg atgaagtcac tagtaccggt cgagaccatc gagaagaaaa
    38221 ttctgctgat tcgcgacgaa aaggtgatgc tggatagctc tctggcaata ctttatggcg
    38281 ttgagactcg cgagatcgta caggctgtga aaagaaacat tggaagattt cccgatgact
    38341 tcatgttcca gcttacagcc gaggagttcg accttttgag atcacagtct gtgatctcaa
    38401 agcccgttgg ccgtggcggc cgccgttacc tgccctacgc ctttaccgag cagggcgtgg
    38461 cgatgctctc cagcgtgctg aaaagtgaaa gggcaatcaa ggtcaatatc gaaatcatgc
    38521 gatcattcgt caacctaagg cggatgcttt cctcgaatgc ggaactggcc cggaatctgg
    38581 ccgccttaga gaaaaaatac gatcttcaat tcaaggctgt tttcgatgcc atccggcagt
    38641 tgatggcgcc gcctgtcaac ccaaagcgaa aaatcggctt ccatgcggat cgtatcgata
    38701 aataaatgga acgcgtccat accatggata atagcagact gactcaataa aatatgattg
    38761 cctattctgc cggtagtatg gaggacatcg gctatggcgg gtgagtgggt aaataagacg
    38821 cttggtgacg tattgacgct tcagcgtggc ttcgatctgc ctgaaaccga acgagaagca
    38881 ggcatatttc ccgtgattgc atcgacagga agggtcggca cgcattcggt ctcaatggta
    38941 aaaggtccgg gtgtagtgat tgaacgatcc ggaagcctcg gcggagggca gttcattcaa
    39001 agtgattttt ggcctctcaa taccacgctt agggtgaagg acttcaaagg taacgatgct
    39061 cgtttttgtt actacctgtt gaagtccctt gatctcgcta ccttcaacac cggaagcgga
    39121 gttccaacgc ttaaccgtaa tcacattcat ccgctgcctg tccaagtccc atctgatgtc
    39181 gctgagcagc gcgccattgc ccacatcctt ggatcgttgg acgacaagat tgagttgaac
    39241 cgacggatga accggacgct ggaggagatg gcgcgggcga tcttcaagag ctggttcgtg
    39301 gatttcgacc ccgtgcgggc caaggctgcc ggacaacagc ccccaggcct caaacccgaa
    39361 atcgcctccc ttttcccgga ctcctttgaa gactctgaat tgggtgagat tccgaggggg
    39421 tggcgggtcc ttcccatcgg cgaattggca gaggtatcta gtgggaagag accgaatgcc
    39481 cgatttgcgg aacgaaccga tgaggcttcc gtgccccttt ggggtggaaa cggtccgatg
    39541 gcttttgtgc gcaaggcgtt gtttgacgaa ccagttctgc ttacgggtcg agtgggcacc
    39601 cttgggtcgg ttttccgcat cactgaaccg tgttggccat cggataacac tcttgtctta
    39661 caggccaccg agccatccgt ttttcagtat ctgtttcttc aactgcatca ggtgaacttc
    39721 acttcgctta acagagggtc cactcaacct ttactgacgc agactgactt gaaggctcaa
    39781 cggttggtag tatccacaaa aagtatattg gagactttca gccgtatagc tgacagcatg
    39841 tattgtcgtt ttgatgaatc caccgccgaa atcctcaccc tcgtcgctat tcgcgacaca
    39901 ttgctgccca agctcatctc cagcgaattg cggttacccg atgccgagcg aatcaccgag
    39961 agatgtctat gaacgagaac cagcaatttc tgctttatac cgccccggat ggggcggtta
    40021 aggtggaggt gtttttcaag ggcgagacgg tgtggctgac gcataaggcc ctggcggagt
    40081 tgtttggggt caagacgccc gcggtcaaca agcacctgaa aaatatcttc gaatccggcg
    40141 aaatggcgga ggattcagtt atatccattt tggaatcaac tgccgctggc ggcaaaaact
    40201 accagacccg cgattggagg gtaaaaagtg agccccgcat tcagtgaatc tgtcatcgag
    40261 caggttgcgt tggcgtggct ggagagcctg ggctaccagg tcctgtcggg gctggagatc
    40321 gcccccggtg aacctgccgc cgagcgtgat aactacggcc aggtcgtcct ggaatatcgg
    40381 ctgcgccagg cgctggcatg gctcaatccg caggtgccgg cggatgcctt ggaagaagcc
    40441 ttccgcaagc tgacacggcc ggactccccg gcgctcgtgg cgaacaacca cgccattcat
    40501 aagtatctgg tcgagggcgt gccggtcgag tatcagcgga aggatggctc gattggcggc
    40561 gatcttgtcc gcgtgttgaa ttacgacgag ccggaagaca acgaattcct ggcagtcaat
    40621 cagttcacgg tggtcgaaaa tcagatcgaa cggcggccgg atgtggtgtt gttcatcaac
    40681 ggcctgccaa tagcggtgat ggaactcaaa aatgccgcca ccgaaaatgc cactatctgg
    40741 tcggccttca atcagcttca gacctacaaa atgcagattc cgtcgctgtt ctcattcaac
    40801 gaggcgatgg tgatctctga cggcgtgcag tcccggatcg gcacggatcg gcacgctgac
    40861 ggctgatcgt gagtggttca tgccatggcg gaccattgaa ggcgaggatt tggccgatac
    40921 aaagctgccg cagcttcaag ttatgcttga gggagtgttt gagaagcggc ggctcctgga
    40981 cttgatccgg cacttcatcg tcttcgagga tgtgggcggc ggcgtgctgg tcaagaagat
    41041 ggccgagtat caccagtacc acgcggtgaa tgtggcggtg caggagacag ttcgcgcctg
    41101 catgacggtg gcggacgtga ccggtattcg tgatgaagga acctacttta cacgcggcca
    41161 gcgggatgcg aagcggggtg accgccgcgt cggcgtggtg tggcacacgc aggggtcggg
    41221 caagagcctg aagatggcgt tctacgccgg acgggtaatc ctgcatccgg cgatggagaa
    41281 cccgacgctg gtcgtgatca ccgaccgcaa tgacctggac gaccagcttt acggcacgtt
    41341 cgcgcgctgt cacgaattgc ttcgccaaca gcccatgcag gctcaaagcc gggcgcatct
    41401 gcgggagctt ttgacgaccg cttccggtgg agtcgttttc acgacggttc acaagttctt
    41461 tccgtccgag ggcgaggatc aacacccgct gctgtcggac cggtgaaaca tcgtcgtgat
    41521 cgccgacgaa gcgcatcgca gccaatatga cttcatcgac ggcttcgctc ggcatatgcg
    41581 cgaggcgggc cggcgaggcg agaaactggg gctgacggat gatgaggtgg cgttctatga
    41641 tgcgctggag gtcaacgata gcgccgtggc cgtcctcggt gacgagacgc tgcggatgat
    41701 tgctcaagaa ctcgtcaaag cggtccggaa cagcatcacc atcgactgga ccgtccggga
    41761 aaacgtccgc gcccagatgc gggtaatcat caagcgcatt cttcgaaaat acggctatcc
    41821 gccagataag caggcccgcg ccacggagct tgttttggaa caggcagaaa tcatctgtaa
    41881 ggattggtcc gaaaagatat gacaaacgac aaagaggtca agacctttga aaaaccccgt
    41941 tccggcgtcc atattgtcat tcccgtgaga tcggggatac agtattatca agaagttcct
    42001 ggattccgga tcaagcccag aatgacagaa cagggcgttt ttcaaaggtc tcaggtcgtc
    42061 attgctgacg tgtcaatagg tctggatcga ttggtccggg gaccgtttgt cctgccgggg
    42121 cgggacatgg tcatctcctg gtgatgagcc agatgatgag ggtaaggaca atgctggcga
    42181 tgatgctggt ggtaaggggg aagtaaaagg tgaatttttc tccccggtag aagatgtctc
    42241 cgggaagcct gcccagccag ggaagcttgc cgccgagcat gatgaggagg cctacggcgg
    42301 cgacggcaag gccgactatc agtaatatct ttcctaactg tgagaaatcg ttcatcatgt
    42361 tcttgctaca gcgcctcccg gtatgtgacc gtctcgccgg tcaggggagc cgtgaacagg
    42421 taggcgccgt cgtcgccctt gcgaccttcc tgaccggcaa gggggatctt gcctttaccg
    42481 cccgggatgt cgaggatcag ccgggggatc agatagcccg gaagccggcc cgacagcgcc
    42541 cgggagagcc caatggccgc ctccagggga atccggaagt gggagatccc cggggccaga
    42601 tcgggataat ggagataata gggcagaatc cccgcctccc caagggcaag cgacagcccc
    42661 gccagttctt cctccgagtc gttgacgccc ttgagcagga cgctctgatt aagcaggaac
    42721 cttgcccgtc gcagcgccgc cgccgcccgc aggaattccg ccgtgatctc caggcgatga
    42781 ttcacatgga cgacgatcgt gtccagttgc gccgctacgg gaccgaagct ttcacaccgg
    42841 acggggtcat agacgggata acgggtgtgg aggcggacgg agcgcgcccc tgccttcctt
    42901 accgcgccga tgacggccgt gagggtttca tcggccagcg acaggggatc gccgccggag
    42961 aggatgacct catggatttc cccgtgggcg gcgaggtaag cgcaggccgc agccaattcc
    43021 tccggcgtaa gatcggaaga aaaacgactc agcctgtctc ttctaaagca aaagcggcaa
    43081 tgggcggcgc aacggtccgt gaccaagagc agggcacggc cgggatagtg gtggatcaat
    43141 cgcccgttgg caccgcgctg ccagcgtcca tccccgatgg gatccgtcct ttcctccggc
    43201 cggacccgtc gctccagctc ggaatggcgc accatcctgg ccaggggccc atcggggcca
    43261 tcgtggtcga tcaaattccg atagtaaggg gtgatctttt cgacaaacat cggttccggc
    43321 cggcgcctcc tcaaaccgtc aatcggaccg ggtgttcccg agcttgcccc ggacaaaact
    43381 gatgtgggta cgaagcacat agaactggtc tccgtatgcc gccggcacct tcatcttgtg
    43441 gacctc
//
LOCUS       DAOSMF010000005        41158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_1275_length_41158_cov_24.886699, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000005 DAOSMF010000000
VERSION     DAOSMF010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 41158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41158
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1275_length_41158_cov_24.886699"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1197)
                     /locus_tag="PLT63_01595"
     CDS             complement(<1..1197)
                     /locus_tag="PLT63_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOD97443.1"
                     /translation="MTSLLATTRIALRALWVNKMRSALTMLGIIIGVGAVIAMLAVGQ
                     GASQKISEQVASMGSNLLIVMPGSLTSGGLRMGSGAQMTLSLDDAYAIERECPAVAAV
                     APMVSGVAQIVYGNQNWSTAIQGTTPGILPARDITLVSGRPFTDQDVRAATKVVMLGQ
                     TVVENLFGAHDPVGQIVRIQKVPFTVVGVLEEKGQSLMGHDQDDLAYIPVTTAQKRIL
                     GSHFVGSVRSILIKAGSAADMDRAERQITELLKQRHRISPKGELDFTIRNLTQMLQAA
                     EQSTRVMSLLLGAIASVSLLVGGIGIMNIMLVSVTERTREIGIRMAVGAKTWAIRMQF
                     LVEALTLSLSGGVIGIILGVASSEILSRLAGWTTIISPLSVVLAFSFSGFVGVFFGFY
                     PAYKASL"
     gene            complement(1201..1887)
                     /locus_tag="PLT63_01600"
     CDS             complement(1201..1887)
                     /locus_tag="PLT63_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD97444.1"
                     /translation="MIDVQELTKIYQVGDLTVPALTGVTLTIDRGEFVAVMGPSGSGK
                     STFMNILGCLDQLTTGRYLLDGIDVGRFSKDELAEIRNRKMGFVFQGFNLLPRASAQE
                     NVELPMLYSGVPAGERHRRAREALIKLGLEGREHHLPSQLSGGQQQRVAIARALVNEA
                     PLLFADEPTGNLDTKTSLEIMDLLTRLNHEGGITLILVTHEPDIAAFSRRVIRFVDGR
                     VVSDQRQEGG"
     gene            complement(1970..3154)
                     /locus_tag="PLT63_01605"
     CDS             complement(1970..3154)
                     /locus_tag="PLT63_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOD97445.1"
                     /translation="MKKRYLRAALVLLVVVLAAAGAYAFLSGGGKGLKYRTEKAATGD
                     IRATVTATGKIDAVTTVIVGAQTSGMIKAIYVDYNTPVNKGQVLAQIDPATFAAKVEQ
                     ARANLMLARANLEKAEATLQDAKRTRDRYETLFARNLIARSDLDTARTNVETAQAQVS
                     ASRAQVEQARANLKQTEIDLAYTTIRSPVDGMVISRNIDIGQTVAASFQTPTLFNIAQ
                     DLTKMQVKTSVDEADIGRIRMGQPVEFTVDAFPEMVFHGNVSEVRNSPTTVSNVVTYE
                     VIVRVDNPEMKLKPGMTANVSIIIDGKQGVLRVPSAALRFRMPDQGGKRDQGGTKGAQ
                     LWVLAGEKPRRLSVQTGITDGSFTEITGGGLLAGQEVIIEALEANKKQQNTGAQPPRF
                     LR"
     gene            complement(3151..4536)
                     /locus_tag="PLT63_01610"
     CDS             complement(3151..4536)
                     /locus_tag="PLT63_01610"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOD97446.1"
                     /translation="MTSRWIYAFSMPRDGASRKRDTFRSFWGIIKPAVWGLLLGCLLL
                     ALPAWAQEEIIARGETLDLQRCVRIALSRHPTILSAAGAVKVNESRIGQARANYFPQV
                     SASSSYSRVDPYTTPAGTIAQQTGNVYNQYGNSVSLNQTLYDFGKTSAQVDVQTLNAG
                     SARQDFSDARRQIVFGVKQAYYSYLKAKRNKDVAADTVRQFQHHLLQAQGFFEVGTKP
                     KFDVTKAEVDLSNAKLNMIKAENDFRIARVNLNNALGYPDAPDYEAADVEFKLNRRDL
                     DFNAALKRAYDERPDLKSAVTKREAAEKSIGVAKKGYYPNLTGSAGYGWGGQEFPWDK
                     GWNVGATLNVPIFTGLSTKYQVDEARANLEVARASEMTIRQNIYLELKQVYSNYQEVS
                     ERVTTAEIQVKQAVENLELAEGRYASGVGNAIEVTDALVSLNNARTSHAAAVYDYMIA
                     EAGIEKAIGTL"
     gene            complement(4864..6351)
                     /gene="cysS"
                     /locus_tag="PLT63_01615"
     CDS             complement(4864..6351)
                     /gene="cysS"
                     /locus_tag="PLT63_01615"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOD97447.1"
                     /translation="MALKFYNTMTRRKEEFQPLVPGKVGIYACGITAYDLCHIGHARS
                     AIVFDVIVRYMRYRGLNVTFVKNFTDVDDKIIERARVEKCDIYAVSEKYIQTHDRDMD
                     ALGVERPTIAPRATENIDGMLRLITALFERGLAYAVPGGDVYFAVEKFPGYGKLSGRN
                     LEDMQAGARIDVNEKKRHPMDFALWKASKEGEPAWDSPWGKGRPGWHIECSVMSQRYL
                     GDTFDIHGGGEDLIFPHHENEIAQSEGATGKTFARYWIHNGFVRVNSEKMSKSLGNFA
                     TIQDMLQACHPEVLRLFVLQSHYRSPVDFSEESLREARLGMDRLYSTLQILEETLHKY
                     GRAETAPREEALPERDLRVLENIQTLPERFVEAMDDDFNTARALGHLFDAVRVLNGYL
                     AREQHTPGAVAVHVLTTSREILRRTGAVLGLLQEDPERYFDADRDREARKLELDVVEI
                     ENLVAERRRARDRQEWQRADEIRKLLLDKKIVLKDSPDRTTWKIA"
     gene            complement(6380..7420)
                     /gene="argC"
                     /locus_tag="PLT63_01620"
     CDS             complement(6380..7420)
                     /gene="argC"
                     /locus_tag="PLT63_01620"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HOD97448.1"
                     /translation="MIKIGIYGASGYTGQELLRLLLRHPKVEVTAVTSRRYAGRPVAA
                     IYPAFTDLTDLAFMDASPDEVAEGCEQVFLALPHAVSMSVVPRFLRKPGLKVIDLSAD
                     FRLRDPEVYAAWYGRHTAADYLAEAVYGLPELYRKAIRPARLIANPGCYPTSVILGLA
                     PLLKSGWIDPASIIADSKSGVSGAGREPLVASLYCEVAEAFKAYKVAQHRHTPEMEQE
                     LGFLAGQDLRISFTPHLLPVTRGILSTLYASLTSPRTVKDVHDLYEDFYGREHFVRVY
                     GPGDYPNVSSVRGTNYCDLGVAVDGRTGRVLVISAIDNLVKGAAGQAIQNMNVLWGWK
                     EDLGLEMISLYP"
     gene            complement(7466..7864)
                     /gene="rpsI"
                     /locus_tag="PLT63_01625"
     CDS             complement(7466..7864)
                     /gene="rpsI"
                     /locus_tag="PLT63_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004695095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOD97449.1"
                     /translation="MGATDKRFYATGKRKTAVARVYMKPGTGIMMVNKRNFDDYFTRD
                     SLKMIIQQPLEIVGKKDQFDFYINVRGGGSSGQAGAIKHGISKVLTEYDLELRPMLKR
                     AGFLTRDARIKERKKYGQPGARKRFQFSKR"
     gene            complement(7893..8321)
                     /gene="rplM"
                     /locus_tag="PLT63_01630"
     CDS             complement(7893..8321)
                     /gene="rplM"
                     /locus_tag="PLT63_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOD97450.1"
                     /translation="MKTFNIKKEEINRDWYVVDGQGKILGRLASEIARRLRGKHKPVY
                     TPHLDTGDFVIVVNAAKIHLTGKKLTKKVYYRHSGYPGGLKQITAEKLLAEKPTEMLK
                     IAVRGMLPKNTLGRAMLKKLKIYQGPEHPHDAQCPRVLDI"
     gene            complement(8403..10880)
                     /gene="lptD"
                     /locus_tag="PLT63_01635"
     CDS             complement(8403..10880)
                     /gene="lptD"
                     /locus_tag="PLT63_01635"
                     /inference="COORDINATES: protein motif:HMM:NF016348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HOD97451.1"
                     /translation="MHRMITLRLPAGIEKAGHRRFFLATLLLVVLLFPGPARGEDTTV
                     LQGLVGDEKGNPVSGALVETREDPHRGVATGSDGTFSLVVPTGQRRFLLTVNRDGYFP
                     HYEVCDINKWGKDARVILLSPLPSPLATVSSPPGGKAPAVPVDIEADTMSYFQDRDSY
                     QADGNVIIHYQEKILNAATVTLNRTTEEAFAQGGVVLKSKDDVLAGERVKIHFPSHTG
                     EVQEGKMFIAQTHLYIRGDRIEKKGEATYSVENAQATTCDGEKPDWHLAGKTLDVTVD
                     GYGTITHGKFYAGKLPVIYTPYLLFPAKTTRQSGFLFPHRLTYSQDKLGWDIGIPFYW
                     AISKDADATFYQRYMSQRGFQEGAELRYALGSTTTGTIYGDFLNDSKEVTETNGNISR
                     NWKSNQKRWAFYWNQETRFDPSFYFRADVAKVSDSWYFKDFSSHNYFLDNYAADRVQP
                     FKKVAFVGNEALTSLDSTVRLVKNWKLFNLTALVKNTDDYTVATNDGTLQKYPEVTLT
                     GAKAPLFGSPVNYEFTSNYDYFYRDAGQRGQYLDFNPTLSLPWNVGDYFQLTPFAGVQ
                     GTFWWRDDKLEDGLAKRGDREVYKMGANLVSEVQRIFPVGGQTVDKIRHALRPEVTYT
                     YIPNPNQDSLPNYGTVVSEQHTVTYALTNTLISRILEKGGKARYLELLRFKLAQTYDL
                     MAAAASGVPPGTNRQPFSDVEMEIDFKPFSYLSFMARNKYSVYDTNWTQANYDLGLND
                     KRGDTATVTYRYTRDSLEEINLLLKAVITKELNLNLTFKRDLLTEKNIEQTYGFDFRR
                     QCWHIGFSYGDRDNDKIYEFRFGIYGM"
     gene            complement(10889..12178)
                     /locus_tag="PLT63_01640"
     CDS             complement(10889..12178)
                     /locus_tag="PLT63_01640"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HOD97452.1"
                     /translation="MTSSLDPHSYLQRLTALGIRMDLGAVRAALEALDHPESFYPALL
                     IAGTNGKGSVAAMAAAILAAAGYSVGLYTSPHLVDVRERIRVDGEMITQRDLASAIGE
                     IDRWLDEPLTYFEFLTVLAGFHFRKKKIDIAVIEVGMGGRLDATRVFEAPVAVITNVA
                     RDHQEYLGKRLRDIATEKAAIIPAGGICVTAVRPSGARQAIEDMARERGTALWRLGKE
                     IKIRRRRDGTFSYRCEGRYRHALPCNLKGGHQRQNAALAVAAVEAMADRGIIVSDEAI
                     RRGLAGVHWEGRLEVLHRDPVLLIDGAHNPAGIRVLCRALREEFSFRRLIVVFGVLRD
                     KHYREMAERMVSVASMMILTCPESERSLPPTELLPFTGGATREVETITSPVAALRRAF
                     AVAHAEDLILVTGSLYLVGEIKKTFPSLRTMIAPRGK"
     gene            complement(12290..12550)
                     /locus_tag="PLT63_01645"
     CDS             complement(12290..12550)
                     /locus_tag="PLT63_01645"
                     /inference="COORDINATES: protein motif:HMM:NF015126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HOD97453.1"
                     /translation="MAEEKIGEVVKFFAKPSVAAVKIVSGELKIGDTIKITGHTTDIT
                     DTIESMEVNNQKVEKAVPGDYIGVKVPDRVRPGDEVFRILPD"
     gene            complement(12693..13097)
                     /gene="rplQ"
                     /locus_tag="PLT63_01650"
     CDS             complement(12693..13097)
                     /gene="rplQ"
                     /locus_tag="PLT63_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013573706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOD97454.1"
                     /translation="MRHGKADCKLGRTTSHRKAMLRNMVTSFIDHESIQTTDAKAKEL
                     KRVAEKMVTLGKRGSLHARRQALGYVRSAKTVKKLFEELSVRYRERAGGYTRIVKLGF
                     RIGDNAPVSLVEFVKDTDKEKSKKKAGAAKKA"
     gene            complement(13155..14153)
                     /locus_tag="PLT63_01655"
     CDS             complement(13155..14153)
                     /locus_tag="PLT63_01655"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOD97455.1"
                     /translation="MQRNWRGLIRPKRIEIDETTHTRYYGEFTCQPLERGMGVTLGNA
                     LRRTLLSSIQGAAIVSVRFDGVLHEFSTIPGVKEDVTDIILNLKGVRVMLHQDGPRML
                     NLDISREGIVTADDIAHDSTVEILNPDHYLATLSGKTNLKAEMVAKLGRGYVQAKKEK
                     DYDQPEGTINIDAVFSPIKKVNYTVTHARVGQIADYDRLVLEVWTDGSVLPEDAMAFA
                     SKILKEQLDIFINFSEQEEESEQPEIEEKDNLNEQLLRHVDDLELSVRSANCLKNAGI
                     NLIGELVQKTEAEMLKTKNFGRKSLNEIKEILAEQGLGFGMKLDFHPWNSKEDNRE"
     gene            complement(14206..14832)
                     /gene="rpsD"
                     /locus_tag="PLT63_01660"
     CDS             complement(14206..14832)
                     /gene="rpsD"
                     /locus_tag="PLT63_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016801712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOD97456.1"
                     /translation="MARYRESVCRLCRREGLKLFLKGDRCYSEKCAFERREYAPGDHG
                     QMRRKPTDYCVQLRAKQKLRRMYGLLEKQFHGYFEKADRIKGITGTNLLILLERRLDN
                     MVFRMGFANSRTEARQLVRHNHFSVNGKKVNIPSYMVKVGDEIEVREGSRKIARIVEA
                     LESVARRGVPQWLALDKDSFKGAVKMLPTRDELTMPVQEQLVVELYSK"
     gene            complement(14881..15273)
                     /gene="rpsK"
                     /locus_tag="PLT63_01665"
     CDS             complement(14881..15273)
                     /gene="rpsK"
                     /locus_tag="PLT63_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOD97457.1"
                     /translation="MARPARKTGKKKEKKNIPEGIAHIQSTFNNTIVTITDLSGNVVS
                     WSSSGIQGFKGSRKSTPFAAQMAAEDAVKKAKEHGMRSVQVYVKGPGSGRESALRALQ
                     VAGINITLIRDVTPIPHNGCRPPKRRRV"
     gene            complement(15286..15654)
                     /gene="rpsM"
                     /locus_tag="PLT63_01670"
     CDS             complement(15286..15654)
                     /gene="rpsM"
                     /locus_tag="PLT63_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOD97458.1"
                     /translation="MARIAGVDLPKNKRMEIALTYIYGIGRTTAQKILEKAGVDANVK
                     TDNLADAEITAIRNIIDKDHKVEGDLRREVSMDLKRLMDLGTYRGLRHRKGLPVRGQR
                     SHTNARTRKGPRRAIAGKKK"
     gene            complement(15686..15799)
                     /gene="rpmJ"
                     /locus_tag="PLT63_01675"
     CDS             complement(15686..15799)
                     /gene="rpmJ"
                     /locus_tag="PLT63_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOD97459.1"
                     /translation="MKVRASVKKICDKCKIVKRKGILRVICENPKHKQRQG"
     gene            complement(15847..16065)
                     /gene="infA"
                     /locus_tag="PLT63_01680"
     CDS             complement(15847..16065)
                     /gene="infA"
                     /locus_tag="PLT63_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOD97460.1"
                     /translation="MVKEEAIEVEGKVIEPLPNAMFRVELENGHRVLAHISGKMRMHF
                     IKILPGDKVTVELSPYDLTRGRIIYRTK"
     gene            complement(16131..16880)
                     /gene="map"
                     /locus_tag="PLT63_01685"
     CDS             complement(16131..16880)
                     /gene="map"
                     /locus_tag="PLT63_01685"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOD97461.1"
                     /translation="MVILKQRSEIDKIRASNAIVAEVLVALKEKVKEGITTRQLDRLS
                     EELVLKKGAKPAFKGYRGYPFSLCASVNDEIIHGLPSERVLVDGDIIGLDFGACYKGY
                     YGDAALTVPVGTISPEAVRLLQATEESLVLAIREARVGNRLGDISAAVQGHVETAGFS
                     VVRDFVGHGIGRNLHEEPQIPNYGVQGRGIKLKAGMVLAIEPMVNVGSYKIKMRPDGW
                     TAVTADGKLSAHFEHTIAITENGPEILSRLQ"
     gene            complement(16916..18223)
                     /gene="secY"
                     /locus_tag="PLT63_01690"
     CDS             complement(16916..18223)
                     /gene="secY"
                     /locus_tag="PLT63_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOD97462.1"
                     /translation="MIGGLGNISKIPELQKRIFFTFGLLIVYRIGAHVPTPGIDAAAL
                     AAYFEQAKGSLLGLFDMFAGGALSNLSVFALGIMPYISASIILQLLTIAIPHLEKLSK
                     EGEAGRRKITQYTRYGTVVLSIIQGFGIAIGLENMAGPTGASIVLETGWGFRLMTMIT
                     LTAGTAFIMWLGEQITEKGIGNGISLIIFAGIVCRGPEAIISTFQLLSAGEMGIFFIV
                     ILIIMMVAVVGTIVYVESAQRRIPVQYAKRVVGRKVYGGQSTHLPLKVNTAGVIPPIF
                     ASSIIMFPATIANFIPHPWMKQVADMLVPGRMVYELLYIAFIVFFCYFYTAVTFNPVD
                     VADNMKKYGGYVPGIRPGKKTADYIDDVLTRLTFSGAIYVSAVCVLPSILISYFNVPF
                     YFGGTALLIVVGVALDTAGQIETHLLTRQYEGFMKKGKIAKRR"
     gene            complement(18225..18662)
                     /gene="rplO"
                     /locus_tag="PLT63_01695"
     CDS             complement(18225..18662)
                     /gene="rplO"
                     /locus_tag="PLT63_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOD97463.1"
                     /translation="MDLKTLKAPAGSRKKCKRIGRGGAHGGTAGKGHKGQKARSGGGA
                     KPGFEGGQMPMARRLPKRGFRNPFRREIITINLGQLNVFPERSVVDADALMARGLIKK
                     RGDGVKLLGKGEIQYPLSVKLDALSKGARTKIEAAGGSVVEVV"
     gene            complement(18669..18872)
                     /gene="rpmD"
                     /locus_tag="PLT63_01700"
     CDS             complement(18669..18872)
                     /gene="rpmD"
                     /locus_tag="PLT63_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOD97464.1"
                     /translation="MRSSLVGRKLKITLIRSYIGRPEKHRQVLRGLGLMKMNKTVILA
                     DTPEVRGMVNKVCHLVSMEEVEA"
     gene            complement(18880..19359)
                     /gene="rpsE"
                     /locus_tag="PLT63_01705"
     CDS             complement(18880..19359)
                     /gene="rpsE"
                     /locus_tag="PLT63_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003133760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOD97465.1"
                     /translation="MEELELIDRVITINRTAKVVKGGRRFRFSAIVVVGDGQGSVGAG
                     LGKAHEVPEAIRKGMEQAKKNMRKVAIANHTIPYEVIGSYGAGRVLLKPASEGTGLIA
                     GGAVRAVLEAAGVHNILTKCLGSHNPHNLVKATIEGLSRLKEPAEIARLRGQTSLAQ"
     gene            complement(19396..19758)
                     /gene="rplR"
                     /locus_tag="PLT63_01710"
     CDS             complement(19396..19758)
                     /gene="rplR"
                     /locus_tag="PLT63_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOD97466.1"
                     /translation="MAAKVEKIREKRKARVRGKVSGTQIRPRLAVFRTARHIYVQAIA
                     DDLGRTIAEASTLSPELRGELGGKKKSDAAKEVGKLVAKRLISAGIEEAVFDRGRFLY
                     HGRIKALADAAREGGLKF"
     gene            complement(19804..20346)
                     /gene="rplF"
                     /locus_tag="PLT63_01715"
     CDS             complement(19804..20346)
                     /gene="rplF"
                     /locus_tag="PLT63_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008372550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOD97467.1"
                     /translation="MSRIGKKTIEIPVGVKVAQEGRLVKVTGPKGELSAVLPLGIEFM
                     HGENAVSVQRLEENRLGRSAHGLARTLVNNMVIGVSKGFEKGLEISGVGYRAELNNNV
                     LKLVVGFSAPREYVVPQGVSVKIEKQVNILVSGIDKYLVGKVASEIRDLRKPEPYKGK
                     GIRYAGEQVRRKVGKSVGTK"
     gene            complement(20389..20790)
                     /gene="rpsH"
                     /locus_tag="PLT63_01720"
     CDS             complement(20389..20790)
                     /gene="rpsH"
                     /locus_tag="PLT63_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOD97468.1"
                     /translation="MGMTDPIADMLTRIRNANRMHFKTVDVLASRMNRNVAEVLKKAG
                     YISGYELKKEKDKREVLRIELVYPDSRNYVLTDIQRVSRPGRRVYVHSADIPKVLNGY
                     GIAIMSTSKGLMTDREARDLNVGGEIICQVW"
     gene            complement(20813..20998)
                     /locus_tag="PLT63_01725"
     CDS             complement(20813..20998)
                     /locus_tag="PLT63_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HOD97469.1"
                     /translation="MAKKSLIAKAKREIKFSVRQYNRCPLCGRPRAYYRKFDMCRICL
                     RNLAAKGEIPGVIKASW"
     gene            complement(21027..21566)
                     /gene="rplE"
                     /locus_tag="PLT63_01730"
     CDS             complement(21027..21566)
                     /gene="rplE"
                     /locus_tag="PLT63_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOD97470.1"
                     /translation="MARLKDYYQKEVVPALIGKFGYKNRMEVPKLEKIVLNMGLGEAI
                     QNAKVLDTAAAEIAAITGQKPVITKARKSIATFKLRQGMSIGCRVTLRRERMYEFLDR
                     LVNINLPRVRDFRGIAPKSFDGRGNFALGLKEQIIFPEIEYDKIDKIRGMNLVIVTTA
                     KTDDEARELLRLMGMPFRN"
     gene            complement(21577..21897)
                     /gene="rplX"
                     /locus_tag="PLT63_01735"
     CDS             complement(21577..21897)
                     /gene="rplX"
                     /locus_tag="PLT63_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOD97471.1"
                     /translation="MHGKQLKKGDTVQVTAGREKGKTGKIIRIMRDKDRVIVEKLNFV
                     KRHQRPDAKGKGGIVEKEGPIHITNVAYFCSKCEKGVRLGNKILEEGKKVRICKKCQE
                     IIDA"
     gene            complement(21933..22301)
                     /gene="rplN"
                     /locus_tag="PLT63_01740"
     CDS             complement(21933..22301)
                     /gene="rplN"
                     /locus_tag="PLT63_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOD97472.1"
                     /translation="MIQMQTILSVADNSGAKKVACIKVLGGSKRRYASIGDVIVVSVK
                     EAIPNSKVKKGDVMKAVIVRTVKEVRRQDGSYLKFDDNSAVLITNQMEPIGTRIFGPV
                     ARELRAKQFMKIISLAPEVL"
     gene            complement(22334..22591)
                     /gene="rpsQ"
                     /locus_tag="PLT63_01745"
     CDS             complement(22334..22591)
                     /gene="rpsQ"
                     /locus_tag="PLT63_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016660790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOD97473.1"
                     /translation="MEVRGNRRTISGVVVSNKMDKTIVVRVERLVKHSVFHKYIRQFA
                     KYKAHDEANECRVGDRVVIVESRPLSKDKRWRMRSILERAK"
     gene            complement(22594..22785)
                     /gene="rpmC"
                     /locus_tag="PLT63_01750"
     CDS             complement(22594..22785)
                     /gene="rpmC"
                     /locus_tag="PLT63_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOD97474.1"
                     /translation="MKTKELRELQSDELKQKKAELDEELFRLKIRHSSRQLDSTAALG
                     KVRKDIARINTVLKQKEGR"
     gene            complement(22775..23197)
                     /gene="rplP"
                     /locus_tag="PLT63_01755"
     CDS             complement(22775..23197)
                     /gene="rplP"
                     /locus_tag="PLT63_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOD97475.1"
                     /translation="MLMPKRVKYRKPQRGRMTGPAMRGNAIVFGEFGLQATECGWVTA
                     RQIESARVAMTRHVKRGGKIWIRVFPQKSVTKKPAETRMGKGKGAPEEWVAVVKPGMV
                     LYEMLGVTPDIAKEAFRLASHKLPISVKFLTREAADEN"
     gene            complement(23235..23882)
                     /gene="rpsC"
                     /locus_tag="PLT63_01760"
     CDS             complement(23235..23882)
                     /gene="rpsC"
                     /locus_tag="PLT63_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOD97476.1"
                     /translation="MGQKVNPIGLRLGGVKTWKSLWFSEKNYSELLHEDLRIRRYLKQ
                     KLYHAGISRIEIERAANKAKVNIYAARPGIIIGKKGSEIEKLKKDLEKVSSSELIINI
                     LEVRKPEIDAQLVAENVALQLERRIAFRRAMRKGVTSAIKFGAKGIRVNCSGRLGGAE
                     MARAEWYREGRVPLHTLRADIDWGFAEARTAYGKIGVKVLIFLGEILGTKRERER"
     gene            complement(23906..24238)
                     /gene="rplV"
                     /locus_tag="PLT63_01765"
     CDS             complement(23906..24238)
                     /gene="rplV"
                     /locus_tag="PLT63_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOD97477.1"
                     /translation="MEARAVAKYIRISPQKARLVADLIRGKKAGEAEKILSYTTKYGA
                     FLLGKVLHSALANAKQNPNIDENILFVKGVYVDQGPALKRWRARAQGRAAAIKKRMSH
                     ITVVLDEA"
     gene            complement(24278..24559)
                     /gene="rpsS"
                     /locus_tag="PLT63_01770"
     CDS             complement(24278..24559)
                     /gene="rpsS"
                     /locus_tag="PLT63_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOD97478.1"
                     /translation="MARSVSKGPFIEPKLLDKVSKAEAAGSKTVIKTWSRRSTITPDL
                     IGHTFAVHNGKKFIPVFVTENMVGHKLGEFAPTRTFFSHAGDRKSKVRR"
     gene            complement(24563..25384)
                     /gene="rplB"
                     /locus_tag="PLT63_01775"
     CDS             complement(24563..25384)
                     /gene="rplB"
                     /locus_tag="PLT63_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOD97479.1"
                     /translation="MAIKTYKPTSPGRRFQTCSDFEEITASIPEKSLLRPLRKSGGRN
                     ANGRMTSRHIGGGHKRRYRIIDFRRDKLDIPARVASIEYDPNRSARIALLNYVDGEKR
                     YILAPDKIAVGDVVISGERADIKAGNSIPLKNIPLGTLIHNVELKVGRGGQIIRSAGS
                     YGQLMAKEGGYAQVRLPSGEVRKIFLDCRATIGQLGNIEHENINIGKAGRTRWLGRRP
                     KVRGVAMNPIDHPMGGGEGKSSGGRHPCTPWGIPTKGHKTRTNKSTDKYIVKRRG"
     gene            complement(25419..25706)
                     /gene="rplW"
                     /locus_tag="PLT63_01780"
     CDS             complement(25419..25706)
                     /gene="rplW"
                     /locus_tag="PLT63_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOD97480.1"
                     /translation="MELYKIIKKTLITEKTTIAKDEANKYFFAVDRRANKIEIGAAVE
                     KLFKVKVLDVAVMNVPGKKKRIGRIVGEKPSWKKAVVTLAPGSRIEIFEGV"
     gene            complement(25706..26329)
                     /gene="rplD"
                     /locus_tag="PLT63_01785"
     CDS             complement(25706..26329)
                     /gene="rplD"
                     /locus_tag="PLT63_01785"
                     /inference="COORDINATES: protein motif:HMM:TIGR03953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOD97481.1"
                     /translation="MPVAAVYDIENNKVAEIELSDQVFGAAVNEAAMYEVVRMQLVTR
                     RLGTAATKTRRDISGGGKKPWRQKGTGRARAGTTRSPIWRGGGTVFGPHPRDYAFKVP
                     RKVRKLALVSALSQKFKEERMIILSDFPLAEVKTKKFKEVVDRFGWKKALVVIDKSNP
                     ILEKSSRNLQEIKMIRSEGINVYDLLNHEHVVLLAPSVKNIEGALRS"
     gene            complement(26348..26986)
                     /gene="rplC"
                     /locus_tag="PLT63_01790"
     CDS             complement(26348..26986)
                     /gene="rplC"
                     /locus_tag="PLT63_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOD97482.1"
                     /translation="MKGLIGRKLGMTQIFTEDGGAVPATVIEVEPSVVVQRKTTEKDG
                     YEAVQLGYGRIKQKHVTKPLQGHFRKADKGFFRTLREFRLEAGGYKPGEELCADIFKA
                     GDYVDITGTSKGKGFAGGVKRHGFGGGRATHGSMFHRAPGSIGASAEPSRVFKGKKLP
                     GRMGGERNTIQNIKVLLVRPEDNALLVKGAVPGGKQGIVLIRSAVKKTTVEK"
     gene            complement(27061..27369)
                     /gene="rpsJ"
                     /locus_tag="PLT63_01795"
     CDS             complement(27061..27369)
                     /gene="rpsJ"
                     /locus_tag="PLT63_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003672692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOD97483.1"
                     /translation="MKDQRIRIRLKAYDYKLLDRSVEDIVETAKRTGARVAGPIPLPT
                     EINRFCVNRSPHVDKKSREQLEIRTHKRLIDIIEPTQSTVDALMKLDLSAGVDVEIKL
                     "
     gene            complement(27392..28585)
                     /gene="tuf"
                     /locus_tag="PLT63_01800"
     CDS             complement(27392..28585)
                     /gene="tuf"
                     /locus_tag="PLT63_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOD97484.1"
                     /translation="MAKKKFERTKPHLNIGTIGHVDHGKTTLTAAITKHLSKKGFAEF
                     RSFDSIDNAPEEKERGVTINITHVEYETDKRHYAHVDCPGHADYIKNMISGAAHMDGT
                     ILVVAASDGPMPQTREHILLARQVQVPYVVVYMNKVDLVDDPELLDLVELELRELLKE
                     YQFPGDDIPIIRGSALKALETDDPNSEEAKSIWELMNAVDNYIPEPQRVLDKPFLMPI
                     GDVFSISGRGTVVTGRVDRGIIKTGEEVEIVGIRPTFKTVCTGVEMFRKTLDEGRAGD
                     DIGVLLRGTKREEVERGQVVAKPGSITPHTKFKAQVYVLTKEEGGRHTPFFTGYRPQF
                     YFRTTDVTGVALLPEGVEMVMPGDNVEMEIQLITPIAMEEQLRFAIREGGRTVGAGVV
                     SKVIE"
     gene            complement(28669..29139)
                     /gene="rpsG"
                     /locus_tag="PLT63_01805"
     CDS             complement(28669..29139)
                     /gene="rpsG"
                     /locus_tag="PLT63_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOD97485.1"
                     /translation="MPRRREVTKREIIPDPKYNNKMVAQFVNNLLKQGKKSTAESILY
                     GAFDIIEKRVKEPPVDVFEKALSNVKPVIEVKSRRVGGSTYQVPTEVQPSRRMALAIR
                     WLITNAKERAEKTMKEKLAAELIDAANNRGAAIKKREAVHKMAEANKAFAHYRF"
     gene            complement(29156..29527)
                     /gene="rpsL"
                     /locus_tag="PLT63_01810"
     CDS             complement(29156..29527)
                     /gene="rpsL"
                     /locus_tag="PLT63_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOD97486.1"
                     /translation="MPTINQLVRKGRTKIEGKTKSPALVGSPQRRGVCVRVYTSTPKK
                     PNSALRKVARVRLTSGYEVTSYIPGVGHNLQEHSVVLVRGGRVKDLPGVRYHIVRGTL
                     DAVGVQGRKQGRSKYGAKKPN"
     gene            complement(29600..33808)
                     /gene="rpoC"
                     /locus_tag="PLT63_01815"
     CDS             complement(29600..33808)
                     /gene="rpoC"
                     /locus_tag="PLT63_01815"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOD97487.1"
                     /translation="MEDVFTYFEKPKDPIRFNAIKISIASPEKILSWSARPNTNGEVK
                     KPETINYRTFKPERDGLFCAKIFGPVKDYECLCGRYKRMKHRGVICEKCGVEVIQSKV
                     RRERMGHIKLATPVAHIWFLKSLPSRIGNVLDLTLKELEKVLYFEAWIVMDPKTTSLR
                     KKELLSEEEVHELREQYGEDAFEVGIGAEAVRKLLEELDLDQMDGELREELKTAVSDT
                     KKKKLVKRLKVVEALKKSGNKPEWMVLTVLPVIPPDLRPLVPLDGGRFATSDLNDLYR
                     RVINRNNRLKRLMELNAPEIIIRNEKRMLQEAVDVLFDNGRRGRAITGTNKRPLRSLS
                     DMLKGKQGRFRQNLLGKRVDYSGRSVITVGPDLRLHQCGLPKQMALELFKPFVYNRLM
                     DKGYVATVKSAKKMVERESSEVWDALDEVVREYPIMLNRAPTLHRLGVQAFEPVLIEG
                     KAIQLHPLVCTAFNADFDGDQMAVHVPLSIEAQTEARVLMMSTNNILSPAHGKPIIIP
                     SQDIVLGIYYLTRAKTDVLGEGMVFSGPEEVRSAYDAGSVDLHARITVRIDGERKETT
                     VGRIMLYEVIPEGIAFDLINKVMNKKELASLIDFTYRTCGAKTTVLLADRLKDLGFKY
                     ATNSGASIAINNMAVPANKKEIVSRADRDVLKIQKQYMDGLITDGERYNKVIDIWAQA
                     TERIAGEMMGGIASTEVTMVSGEKKRTDSFNPIFMMADSGARGSAAQIRQLAGMRGLM
                     AKPSGEIIETPITANFREGLTVLQYFISTHGARKGLADTALKTANSGYLTRRLVDVAQ
                     DCIITEEDCGTIDGIYVSALMEGGEIIETAGERVLGRVALEEIVDPFTGETLVEANQE
                     IDEVLAERIDKAGIERVSIRSVLTCKSKHGVCTMCYGRDLAHGHLVNIGEAVGIIAAQ
                     SIGEPGTQLTMRTFHIGGTAKFEEHSTLQARHDGVVKFVNLSTVKIQAGGMVDLVVMN
                     RNGEIHVLDEQGRNRGKYPVPYGAHLKVNEGDTVYGPGKDPKTGQERQATLIAEWDPF
                     SIPILAEVDGTIKYGDIIEGKTMQEQVDEVTGLSRKVVIESKGTDVRPRVSIKDKKGR
                     TAKVPGTNMVARHLLPVGANIVVSEGDQVSAGDIIAKIPRETTKTKDITGGLPRVAEL
                     FEARKPKEYAVISEIRGVVSYGKDAKGKRKVIITPDSGEEKEYLIPKGKHVSVQEGDR
                     VQEGEPLMDGIHNPHDLLSIKGEKELARYLVDEVQEVYRLQGVKINDKHMEVIVRQML
                     RRVKIVDPGDTVFIADEQVERHRFQEENDRVASEGGRPAVGKPILLGITKASLSTQSF
                     ISAASFQETTRVLTEASLAGKTDYLRGLKENVIMGRLIPAGTGLPMYRKLGIKTLADE
                     VADRAEEQETQELEGNSDEKILDM"
     gene            complement(33820..37911)
                     /gene="rpoB"
                     /locus_tag="PLT63_01820"
     CDS             complement(33820..37911)
                     /gene="rpoB"
                     /locus_tag="PLT63_01820"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOD97488.1"
                     /translation="MGEFRKNFGRIKKIVDIPNLIDIQVKSYEKFLQKDTAPDQRGNY
                     GLQGAFKSVFPISDYSKKCSLEFVSYKIGEVRYDVKECIQKGMSYAAPLKIVVRLLIF
                     DVDLSTNQKQISNIKEQEIYFGEIPLMTENGTFIVNGTERVIVSQLHRSPGIFFDHDK
                     GKTLASGKLIYSARVIPIRGSWLDLEFDSKDLLYVRIDRRRKMPVTILLKAMGNSTEF
                     ILNYFYNIETVFLDGDQFFMAVDNSLLGEKAPEDIRDASDEVIVKKGRKINKILLKKM
                     SEASITRITASEADVVGKILSTDIMDPETGEVLLRCNEELSPEGLKLLQAKNIREVRF
                     IHLDEEMGNVSIRETLLADRIETMNDAIIEIYRRLRPSNPPTPETAQAFFRSLFFEPE
                     TYDLSDVGRAKMNYKLRLDVPTNVTVLRNEDILAAVKYLIDLKNGIGECTVDDIDHLG
                     NRRVRSVGELIENQYRIGLVRMERAIKEKMSLQDIETMMPHDLVNAKPVSAVVNEFFG
                     SSQLSQFMDQTNPLSEITHKRRLSALGPGGLTRERAGFEVRDVHPTHYGRICPVETPE
                     GPNIGLIVSLSTYARVNEFGFIETPYRLVKDGRVLDEVRYMTAIEEENFIIAQADRPL
                     DKDGRFIGEMISARKGGDFITVEPEEINYMDVSPNQLVSVAATLIPFLEHDDANRALM
                     GSNMQRQAVPLMRPAVPVVGTGMEAVVARDSGAVVAARRDGVVESVDAARIVVKCDGT
                     GDDNLDIGVDIYNLVKYQRSNQDTCFNQKPIVVKGDRVRRGDIIADGPATDNGELALG
                     RNVMVAFMSWGGYNYEDSILVSERVVKEDIYTSIHIEEFETMSRDTKLGKEEITRDIP
                     NVGEEALKNLDESGIVRMGVSVKPGDILVGKITPKGETQLSSEEKLLRAIFGEKASDV
                     RDTSLRVPPGVEGTVIDAKIFTRKGVEKDSRSQEIEDEALSSIEKDRNDELRIITDSV
                     RARITEMLVGKTAGAKLADGKKKKIYLKKGEEIDGEILAKIPFDLWKDITLAGDEETE
                     EQVRMLCDNLTRKKERIDAYFKEKIEKLRAGDELPPGVIKMVKVYVAIKRKLSVGDKM
                     AGRHGNKGVLSRILPEEDMPYFVDGTPVDIVLNPLGVPSRMNVGQILETHLGWAAKGL
                     GDKINELMKTRQNLEQVKKEIKAIYASPEANVFIDKASEGDVKRFVSRLQSGISFSTP
                     VFDSADENEIRQMLKLANLPEKGQTVLFDGRTGEPFDQEITVGMIYMLKLHHLVDNKI
                     HARSIGPYSLVTQQPLGGKAQFGGQRLGEMEVWAMEAYGAAYSLQEFLTVKSDDVAGR
                     TRMYETIVKGEHTLEPGLPESFNVLVKELQSLCLDVELIEEES"
     gene            complement(37956..38342)
                     /gene="rplL"
                     /locus_tag="PLT63_01825"
     CDS             complement(37956..38342)
                     /gene="rplL"
                     /locus_tag="PLT63_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOD97489.1"
                     /translation="MASQITKEDVVKFIEGMTVLELSELVKELEQRFGVSAAAPVAVA
                     AVPAGGEAVAPAEEKTEFDTILTGFGDQKIQVIKVVRAITGLGLKEAKDLVDGVPKPI
                     KEAVSKDEAADIKKKIEEVGGTVEIK"
     gene            complement(38369..38893)
                     /gene="rplJ"
                     /locus_tag="PLT63_01830"
     CDS             complement(38369..38893)
                     /gene="rplJ"
                     /locus_tag="PLT63_01830"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOD97490.1"
                     /translation="MDRKEKEQVVAELHEKLKGFKLAVLTGYSGMDVARITALRVALR
                     KSGTDMRIVKNTLLRIASQGTDFSAMNEHIKGPLALVLTDKDVVETVKVLVEFAKKNS
                     EMEIRAAMLEGKLLNREQVNAVALLPSREILIGRLLSVLIGVPTGLVSVLSAVPRGMV
                     QVLDAYREKKESLQ"
     gene            complement(39169..39867)
                     /gene="rplA"
                     /locus_tag="PLT63_01835"
     CDS             complement(39169..39867)
                     /gene="rplA"
                     /locus_tag="PLT63_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOD97491.1"
                     /translation="MTTRGKTFIEAKKKVEPGKRYALKEAVELVIATKRAKFDETVEI
                     AMRLGVNPQHADQMVRGSVVLPNGLGKTVRVLAFVKGDKEREAQEAGADYAGADDLIE
                     KIKGGWLEFDRVVATPDMMGSVGKLGKILGPRGLMPNPKVGTVTFDVARAIGELKAGK
                     VEFRVERAGIVHTPVGKVSFGADKLMENIAAVIETIVKLKPAASKGTYLKSISLSSTM
                     GPGVKVDPLDVRNI"
     gene            complement(39891..40316)
                     /gene="rplK"
                     /locus_tag="PLT63_01840"
     CDS             complement(39891..40316)
                     /gene="rplK"
                     /locus_tag="PLT63_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041584588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOD97492.1"
                     /translation="MAKKVIANIKLQIKAGKATPSPPIGPALGQHGVNIMEFCKAYNA
                     MTQSQEGTVIPVVITVYADRSFTFITKTPPAAVLLKQAAKIAKGSGIPNKEKVGTVTA
                     KQIKEIAELKFNDLNAVDIEGAISVITGTARSMGITIEG"
     gene            complement(40342..40872)
                     /gene="nusG"
                     /locus_tag="PLT63_01845"
     CDS             complement(40342..40872)
                     /gene="nusG"
                     /locus_tag="PLT63_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOD97493.1"
                     /translation="MAQKWYVVHTYSGYENRVKLSLQERIEAAGKQEFFSDVLIPEED
                     IVELVSGEKKTSKRKFFPGYILVRMELNDDTWHIVKDTPKVTGFIGGKEKPFPIPDKD
                     VESLRTRIDEGTLKPKPKFKFEVGDHVRIIDGPFTNFDGVIDEVKPEKGKLRVIVSIF
                     GRSTPVELDFIQVVQN"
     gene            complement(40877..41080)
                     /gene="secE"
                     /locus_tag="PLT63_01850"
     CDS             complement(40877..41080)
                     /gene="secE"
                     /locus_tag="PLT63_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOD97494.1"
                     /translation="MDKIKVWIDKVKVFLSEAKVELKKVTWPTPKQTLASTAVVIVVT
                     AIVSAFLGIVDFGLTKLIKLLLG"
BASE COUNT         8023 a        12306 c        10821 g        10008 t
ORIGIN      
        1 cagcgacgcc ttgtaggcgg gatagaagcc gaaaaaaaca ccgacgaaac cggaaaagct
       61 gaaggccagc acaacggaaa ggggcgagat gatggtggtc cagcccgcca gccgggagag
      121 gatttccgag ctggccaccc cgaggatgat cccgatcacc cccccgctca gagagagggt
      181 cagggcctcc acgagaaact gcatgcggat cgcccaggtc ttggcgccga cggccatgcg
      241 gatgccgatt tcccgcgtcc gttccgtaac ggagaccagc atgatattca tgatcccgat
      301 gccacccacc aggagggaaa cggaggcgat ggccccgagc agcagcgaca tgacccgggt
      361 ggattgctcc gccgcctgga gcatctgggt gagattgcgg atagtgaaat ccaactcccc
      421 cttcggactg atccggtgcc gctgcttgag aagttccgtg atctgccgct ccgcccggtc
      481 catgtccgcc gcagaaccgg ccttgatgag gatggagcgt acggagccga cgaaatgaga
      541 gcctaagatc cgcttctgcg ccgttgtcac ggggatatag gccaggtcgt cctgatcgtg
      601 ccccatgaga gattgcccct tttcttccag gacgccgacc acggtgaagg gcaccttctg
      661 gatacggacg atctgcccga cgggatcatg agccccgaag agattttcca ccaccgtctg
      721 ccccagcata acgaccttgg tcgccgcacg gacatcctgg tccgtaaagg gccgcccgga
      781 aacgagggta atgtcccggg ccgggaggat gcccggagtg gtaccctgga tcgcggtgga
      841 ccagttctgg ttgccgtaaa cgatctgggc gaccccgctg accatggggg ccacggcggc
      901 cacggcggga cactcccgct cgatggcgta ggcgtcgtcc aagctgaggg tcatctgggc
      961 gccgctgccc atacggaggc cgccggaggt aaggctgccg ggcatgacga tgaggaggtt
     1021 gctccccatg gaggccacct gttcggagat cttctgactc gccccctgcc cgacggccag
     1081 catggcgatc acggcgccga cgccgatgat gatccccagc atcgtaaggg cggagcgcat
     1141 cttgttgacc cagagtgccc ggagggcgat tctggtcgtg gcgaggaggc tcgtcatatc
     1201 ttaccccccc tcctgccgct ggtcgctcac gacacggcca tcgacgaagc ggatgacccg
     1261 ccggctgaac gccgcgatat ccggctcgtg agtcaccagg atcagggtaa tgcctccctc
     1321 gtgattgaga cgggtcaaaa gatccatgat ctccaggctg gtttttgtat ccagattccc
     1381 cgtcggctca tcggcgaaca ggaggggggc ctcgttgacc agggcccggg caatggccac
     1441 ccgctgctgc tggcctccgg aaagttggct gggaaggtgg tgctcccgtc cttccagccc
     1501 gagttttatc agtgcttccc gggcgcggcg atgccgttcc ccggcgggaa cgccgctgta
     1561 gagcatgggc agttccacgt tctcctgggc cgaggcccgg ggcagaagat tgaaaccctg
     1621 aaagacaaag cccattttcc gattcctgat ctccgccagt tcatccttgg aaaaacggcc
     1681 gacatcgatg ccgtccagca gataacggcc cgtagtgagc tgatcgaggc agcccaggat
     1741 attcatgaag gtcgatttgc ccgatccgga aggtcccatg acggccacaa actcccccct
     1801 gtcaatggta agggtgacgc cggtgagggc cggcacggtt aaatcgccga cctggtaaat
     1861 cttggtaagc tcctgaacgt cgatcacggt ttccctcaat ccggttcata aaggcgaaac
     1921 acggagaatg catccggcca aaatcatgtc caaaccgacg gcggggcggc taacggagga
     1981 agcgcggcgg ttgggcaccg gtgttctgct gcttcttgtt cgcctccaga gcttcgatga
     2041 tcacttcctg gccggcgagc aggccgccgc cggtgatctc ggtaaaactg ccgtcggtga
     2101 tccccgtctg gacggaaaga cgccgtggtt tctccccggc aaggacccag agctgcgctc
     2161 ccttggttcc gccctgatcg cgcttccccc cctggtcagg catccggaaa cgcagggccg
     2221 cactgggaac ccgcaggaca ccctgcttgc cgtcgatgat aatggatacg ttggccgtca
     2281 ttcccggctt gagtttcatt tccggattgt cgacccggac gatgacctcg taggtcacca
     2341 cattagatac cgtagtgggg gagtttctca cctccgaaac attgccgtga aaaaccattt
     2401 ccggaaaggc atccacggta aactccacgg gctggcccat tcggatgcgg ccgatatccg
     2461 cctcgtccac actggtcttc acctgcatct tggtaagatc ctgagcgatg ttgaacaggg
     2521 tgggggtctg gaagctcgcc gccacagtct gaccgatatc gatgttccgg gagatgacca
     2581 tgccgtccac gggagagcgg atcgtagtat aggccagatc tatttccgtc tgtttgaggt
     2641 tggcacgggc ctgttcgacc tgagctcgtg aggcgctgac ctgggcctgg gccgtctcca
     2701 cattggtgcg cgccgtgtcg agatcgctcc gggcgataag gttgcgggca aagagggttt
     2761 catatctgtc ccgggttctt ttggcatctt ggagagtcgc ctccgccttt tcgagattgg
     2821 cccgagccag catgaggttg gcacgggcct gctcgacctt ggcggcaaag gtggccggat
     2881 caatttgagc aagcacctgt cccttgttga ccggggtgtt gtaatccaca tagatggcct
     2941 tgatcattcc cgaggtctgg gcgccgacaa tgacggtggt gacggcatcg atcttacccg
     3001 tggccgttac cgtcgcccgg atgtcgccgg tggcggcctt ttccgtccgg tatttcaacc
     3061 ccttgccgcc gccgctcagg aaggcgtaag ccccggccgc cgcaaggacg acgaccagca
     3121 gtaccagggc ggcacgcagg tatcttttct tcatagcgtc cctatcgcct tctcgatgcc
     3181 agcctcggca atcatatagt cataaaccgc cgccgcatgg gaagtccggg cgttgttcag
     3241 ggagacgagg gcgtcggtca cctcgatggc gtttcccacc ccggaggcgt agcggccctc
     3301 agcaagctcc agattctcca cggcctgttt cacctggatc tccgccgtgg ttacccgctc
     3361 ggatacctcc tgataattgg aatagacctg cttcaattcc agatagatat tctgccggat
     3421 ggtcatttcg ctggccctgg cgacttccag attcgcccgg gcctcatcga cctgatattt
     3481 cgtggaaaga ccggtaaaga tagggacgtt caatgtggcg cctacattcc agcccttgtc
     3541 ccagggaaac tcttgccccc cccatccgta tcccgccgaa cccgtgagat tgggataata
     3601 gcctttcttc gccaccccga tggatttctc tgccgcttcc cgcttcgtca ccgccgactt
     3661 gagatccggc cgttcatcgt aagcccgttt caaggcggcg ttaaaatcca gatcccgtcg
     3721 attcaacttg aattccacat ccgccgcttc ataatccgga gcgtcgggat aacccagggc
     3781 attgttcaga ttgacccggg cgatccggaa atcattttcc gctttgatca tgttgagctt
     3841 ggcgttgctc agatccacct ccgccttggt gacgtcgaat ttgggtttgg taccgacctc
     3901 gaaaaaaccc tgtgcctgga ggaggtgatg ctggaactgc cggaccgtgt cggcggcgac
     3961 atccttgttt ctcttggctt tgagataact gtaataggcc tgtttcacgc cgaagacgat
     4021 ctggcgacgg gcgtcactga aatcctggcg ggcggaaccg gcattgagtg tctggacgtc
     4081 cacctgcgcc gacgtcttcc cgaaatcata gagggtctga ttgagggaaa cgctgttgcc
     4141 gtattgattg taaacattgc cggtctgctg agcgatggtg ccggcgggcg tggtgtaagg
     4201 atcgacccgg gagtagctgg atgaggcgct cacctgggga aagtaattgg cccgggcctg
     4261 gccgatgcga ctttcgttga ccttgacggc gccggccgcg gacagaatcg ttggatgccg
     4321 ggacaaggcg atccggacac agcgctgtag gtccagggtc tcccccctgg cgataatttc
     4381 ctcctgcgcc cacgccggca gggcgaggag caggcagcca agcaatagtc cccaaaccgc
     4441 cggctttatt attccccaaa acgaacggaa agtgtcccgc ttgcgggaag cgccgtcccg
     4501 gggcatggaa aatgcgtata tccaccgcga tgtcatggcc gtgcagcctt ctttaggaaa
     4561 gatagctttt tttgccgcaa atcggtttct ttcatcttca gcaaccaccg gccataaggg
     4621 gcattcctgc cggatgcgac ggcgccggtc tttttgccgg tcgcctgcat cctcgggcat
     4681 cgggatataa atatagcaac gattgcgcca tccaggcgaa cgtattatac gtaactgatt
     4741 taaaacaatt atttgccggg acttcagttt tctgtcccgg agcgccactg tgagtcgtaa
     4801 aaatatggct aattattatc ccgatcgtct cctcatctgg ctaataatta gccaccggtg
     4861 gggctaagca atcttccagg ttgtccggtc ggggctgtct ttgaggacga tttttttgtc
     4921 cagcagcagt tttcggatct catccgcccg ctgccactcc tgccgatccc gggcccggcg
     4981 gcgctccgcc accaggttct cgatctccac aacatccagt tccagcttcc gggcttcccg
     5041 atcccggtcc gcgtcgaaat accgctcagg gtcttcctgt agcagcccca gaaccgcgcc
     5101 ggtacgccgc agaatctccc gggaagttgt caggacatgg acggcgacgg cgccgggtgt
     5161 atgctgctcc cgggccagat aaccgttgag aacccggacg gcgtcaaaca gatgtcccag
     5221 cgcccgggcg gtattgaaat cgtcatccat ggcctcgaca aaacgttcgg gcagtgtttg
     5281 gatattttcc agcacccgga ggtctctctc cggcagtgcc tcctcccgtg gtgccgtttc
     5341 ggcacgcccg tatttatgca gggtttcttc caggatctgc aatgtggaat agagacgatc
     5401 catgcccaaa cgggcctccc gcagcgattc ctccgaaaag tccaccggac tccgataatg
     5461 gctctggagg acgaacaggc gcaacacttc gggatggcaa gcctggagca tatcctgaat
     5521 ggtagcgaaa tttcccagtg atttcgacat cttttcgctg ttcacccgaa cgaaaccgtt
     5581 gtggatccaa tagcgggcga aggtcttgcc cgtagccccc tcggactggg cgatctcgtt
     5641 ttcatggtgg ggaaagatca ggtcttcacc cccgccgtga atgtcgaagg tatcaccgag
     5701 atagcgctgg ctcatgacgg agcactcgat atgccaccca ggacggccct taccccaggg
     5761 actgtcccag gcgggctccc cctccttgct cgccttccaa agggcgaaat ccatgggatg
     5821 gcgcttcttt tcgttgacat cgatgcgggc tccggcctgc atgtcttcga gattccgccc
     5881 ggacagtttc ccgtaaccag ggaacttttc cacggcaaaa tatacatcac cgcccgggac
     5941 ggcataggcc agtccccgct cgaaaagggc ggtgatcaag cggagcatgc cgtcgatatt
     6001 ctccgtcgcc cggggggcga tggtcggccg ctccactccc agggcgtcca tgtcccgatc
     6061 gtgggtctgg atgtattttt cggagacggc atagatatcg catttctcca cccgggcgcg
     6121 ctcgatgatc ttgtcgtcca catcggtaaa attcttgacg aaggttacat ttagaccccg
     6181 gtagcgcata tacctgacga tgacatcgaa gacaatcgcc gaacgggcat ggccgatgtg
     6241 gcagaggtca taggccgtga tgccgcaggc gtagatccca accttgccgg gaacaagagg
     6301 ctgaaactcc tctttcctgc gggtcatggt gttgtagaat ttcagtgcca tagcagttcc
     6361 tcttaaaaaa gtccggcctt cagggataca gggaaatcat ctccaacccg agatcttcct
     6421 tccatcccca caggacattc atgttctgga tggcctgtcc cgccgcccct ttgaccaggt
     6481 tgtcgatggc ggagatcacc aggacccgcc ccgtccgccc gtccacggca acaccgagat
     6541 cgcagtaatt ggttccccgc acggaagata cgttgggata atctccaggc ccgtaaaccc
     6601 ggacgaaatg ttcccggcca tagaagtctt catagaggtc atgaacatcc ttgacggtcc
     6661 ggggcgatgt cagggacgca taaagggtgc tgaggatgcc ccgggtgaca ggcaggagat
     6721 ggggggtgaa ggagatccgg aggtcctgtc cggccaggaa gcccaattcc tgttccattt
     6781 ccggggtatg gcgatgctgg gcgaccttgt aggccttgaa ggcctcggcg acttcacagt
     6841 acagagaggc gaccagtggc tcacggccgg cgccgctaac cccggatttg gaatcggcga
     6901 tgatggaagc gggatcgatc caccccgact tcaacagcgg ggccagcccc aggatgacgc
     6961 tggtgggata acagcccgga ttggcaatga ggcgcgccgg acggatcgcc ttccgataaa
     7021 gttccggcag gccgtaaacg gcctcggcaa ggtaatcagc ggcggtatga cggccgtacc
     7081 aggcggcata gacctccggg tcccgaaggc ggaaatcggc gctcaggtcg ataaccttca
     7141 gacccggctt ccggaggaac cgggggacga cgctcatgga aacggcatgg ggcagggcca
     7201 gaaagacctg ttcacaccct tcggcaacct catccggcga ggcgtccatg aaggcgaggt
     7261 ccgtcaaatc cgtaaaggcg gggtagatgg ccgcgacggg gcggccggcg taacgacgcg
     7321 aggtcaccgc cgtgacctcc acctttggat ggcgcaggag caggcgcagc agttcctgcc
     7381 cggtataacc gctggccccg taaatgccga tcttgatcat cttgctcccc ccaaaaaaaa
     7441 gggaggcctg aggccccccg agtaatcagc gtttagagaa ttggaacctc tttctggccc
     7501 cgggctgtcc gtatttttta cgttccttga tgcgggcgtc ccgggtcagg aatccggccc
     7561 ttttaagcat ggggcgcaac tccaagtcgt attcggtcag gactttcgaa atgccgtgct
     7621 tgatggcccc ggcctgaccg gagctgccgc cgccccggac attgatgtaa aaatcgaact
     7681 ggtctttctt gcccactatc tccaggggct gctggatgat catcttcagg ctgtcgcgag
     7741 tgaaatagtc gtcaaaattc cgtttgttga ccatcatgat ccccgtcccc ggcttcatgt
     7801 aaaccctggc tacagccgtc ttcctcttgc ccgtggcata aaaacgcttg tcggtggcgc
     7861 ccatttttgt tttccgttcc tccagtaatt atttagatat cgagcacacg cgggcactga
     7921 gcatcatggg gatgttcggg cccctggtaa attttcagtt tcttcagcat tgcccgcccc
     7981 agggtgtttt taggcaacat tccccggacg gcaattttca acatctccgt gggtttttcg
     8041 gcaagcagtt tctccgccgt gatctgtttc aatccgccgg gatatcccga gtggcgataa
     8101 taaacctttt tcgtgagttt cttgcccgtc aggtggatct tggcggcgtt gacgacgatg
     8161 acgaagtccc ccgtatcgag atggggggtg taaacgggct tgtgtttgcc ccggagccgc
     8221 cgggcgatct cgctggccag ccggcccagg atctttccct ggccgtccac tacataccag
     8281 tctctattga tttcttcctt tttgatattg aaggttttca tgtccgtatc accaaattca
     8341 taaaatgaag ctggaaacct acgcaatttc tccctttttg tcaaggaaaa acatccgtaa
     8401 atttacatgc cgtagattcc gaagcggaat tcataaatct tgtcgttgtc ccgatcgccg
     8461 taactgaagc cgatatgcca gcattggcgg cggaaatcga acccgtatgt ctgctcgatg
     8521 tttttttccg tgagtaggtc ccgcttgaag gttaagttga gattgagctc cttcgtgatc
     8581 acggctttca ggaggagatt gatttcttcc agagaatccc gggtatagcg gtaggtcacc
     8641 gtggcagtgt ctcccctttt gtcgttgaga ccgagatcgt aattggcctg ggtccagttc
     8701 gtgtcgtaaa cactatattt gttccgggcc ataaagctca aataggaaaa cggcttgaag
     8761 tcgatctcca tctccacatc gctgaagggc tggcggttcg tgccgggcgg aacaccactg
     8821 gcggcggccg ccatgaggtc ataggtctga gccagtttga agcgcagcaa ttccaggtac
     8881 cttgccttac cccctttttc cagaatccgg ctgatgaggg tattggtcag ggcgtaggtg
     8941 acggtgtgct gttcgctcac taccgtgcca tagttgggca ggctgtcctg attgggattg
     9001 ggaatgtagg tataggtaac ttcggggcgc agggcatggc ggattttatc cacggtctgc
     9061 ccccccaccg gaaagatccg ctgcacttcc gaaacgaggt tggcgcccat tttataaacc
     9121 tcccggtcgc cccgtttggc caggccgtct tccaacttat cgtcccgcca ccagaaggtc
     9181 ccctggacgc cggcgaaggg ggtcaactgg aaataatcgc ccacattcca cggtagggag
     9241 agggtggggt tgaagtccag atattgtccc cgctgaccgg catcccggta aaaataatca
     9301 taattcgatg tgaactcata attgaccggg gagccgaaga gcggggcctt ggcaccggtc
     9361 agggtcactt ccggatattt ttgcagggtg ccgtcattgg tcgccacggt ataatcgtcg
     9421 gtgtttttca ccagggcggt aagattgaac aatttccagt tcttgacgag gcgcaccgtg
     9481 gaatccaggg aggtaagcgc ctcgttaccc acaaaggcaa ccttcttgaa gggctgcacc
     9541 cggtcggcgg cataattgtc caggaagtag ttgtgcgaag agaagtcctt gaaataccaa
     9601 ctgtccgata ccttggcgac atcggcgcga aaatagaagc tggggtcgaa gcgcgtttcc
     9661 tgattccagt agaaggccca gcgcttttgg ttcgatttcc agtttctgct gatgttgccg
     9721 ttggtttccg tcacttcctt gctgtcgttg aggaaatccc cgtaaatggt tcccgtggtg
     9781 gtgctgccaa gggcatagcg cagttccgca ccctcctgga atccccgctg gctcatgtag
     9841 cgctgataaa aggtggcatc cgcatctttg gagatggccc agtaaaaggg gatgccgatg
     9901 tcccagccca atttatcttg ggaataagtc aggcgatggg gaaagaggaa tcccgactgt
     9961 cgggtggtct tggcggggaa aagcaggtaa ggggtataaa tcaccggcaa tttgcccgcg
    10021 tagaacttgc cgtgggtgat cgttccgtag ccatcgacgg tgacatcgag ggttttgccc
    10081 gccagatgcc agtcgggttt ctctccgtca catgtggtcg cctgggcgtt ttctaccgaa
    10141 tatgtcgcct cgcccttctt ttcgatacga tcgccgcgga tgtacaaatg agtctgggcg
    10201 atgaacatct ttccttcctg tacctcgcct gtatgggagg gaaagtggat tttcacccgc
    10261 tctcccgcca ggacatcatc cttgctcttc aacaccactc ccccctgggc gaatgcttcc
    10321 tccgtcgttc tgttgagggt aacggtagcg gcgttcagga tcttttcttg ataatggatg
    10381 atgacgttgc cgtcggcctg gtaggaatcc ctatcctgga aataactcat ggtgtcggct
    10441 tcgatgtcca cgggaacggc cggagccttg ccacccggag gggacgaaac agtggccaat
    10501 ggggaaggta aaggggataa aagaatcacc cgcgcatctt tgccccactt gttaatatcg
    10561 cagacttcat aatggggaaa gtaaccatcc cggttcacgg tcaggagaaa ccggcgctgc
    10621 ccggtgggaa ccaccaggga aaacgtcccg tcagaaccgg tcgccacccc tctgtgggga
    10681 tcttcccggg tctctacaag ggctccggag acgggatttc ccttttcgtc acccactaat
    10741 ccttgcaaga cggtcgtatc ctcgccgcgg gccggaccgg gaaacaacaa aacaaccagc
    10801 agcaatgttg ccaggaagaa ccggcggtgg cccgccttct cgatcccggc cggaagccgc
    10861 agcgttatca tcctatgcat gacggggatc atttccctct gggagctatc atggttcgca
    10921 gggaagggaa agtttttttt atctccccca ccagatacag ggaaccggtc accaaaatta
    10981 ggtcctcggc atgggcaacg gcaaaggcac gccggagggc cgcgaccggt gatgtgatcg
    11041 tctcgacctc ccgggtcgcc cccccggtga aagggaggag ctccgtcggc ggcagggaac
    11101 gttcgctttc tggacaggta agaatcatca tggatgcaac tgagaccatt ctctccgcca
    11161 tctcgcggta atgcttgtcc cggagaacgc cgaacaccac aatgaggcga cggaaagaaa
    11221 attcctcccg cagggcccgg caaaggactc gtatccccgc cgggttatgg gcgccgtcga
    11281 tgagaagaac cggatcgcgg tgcaggacct ccaaacgccc ctcccagtgc acccccgcca
    11341 acccccggcg gatggcttcg tccgacacga tgattccccg gtccgccatg gcctccaccg
    11401 ccgctacggc cagcgcggca ttctgacgtt gatgccctcc cttcaaattg cagggcaggg
    11461 cgtgccgata ccggccctcg cagcgatagg aaaaggttcc gtcccggcga cggcggatct
    11521 tgatttcttt gcccagtcgc cacagggccg tcccccgttc cctcgccatg tcttcgattg
    11581 cctgccgggc gccggaagga cggacggcgg tcacacagat gccgccggca ggaataatcg
    11641 ccgctttttc agtggcgata tcccgaaggc gctttcccag atactcctga tgatcccgag
    11701 ccacattggt gatgaccgcc accggcgcct caaaaaccct tgtcgcatcc agtcggccgc
    11761 ccatgcccac ctcgatcacg gcgatgtcga tctttttctt tcggaaatga aaacctgcca
    11821 aaaccgtgag aaattcaaaa taggtcagcg gttcgtcaag ccagcggtca atctctccga
    11881 ttgccgatgc cagatcccgc tgggtaatca tttcgccatc aacccggatc ctttcccgga
    11941 catccaccaa gtgtggagag gtgtaaagac cgacggaata cccggccgcc gctagaatcg
    12001 cggccgccat ggccgctacg gaacctttac cgttggttcc ggcaataagg agtgcgggat
    12061 agaatgactc cgggtggtcc agggcctcca gggccgcccg aacggcgcca agatccatgc
    12121 ggatgcccag agcggtcaaa cgttgtagat aggaatgggg atcaagggaa gacgtcatcg
    12181 aataataaag gaaaagccat ggaaaaacag gtaaaagtca atccatggct tcaatcgccg
    12241 ccatctgtta tgcacattaa tcacctacta gaggcgattt aggcgccggt taatccggga
    12301 gtatgcggaa aacctcgtcc ccgggacgga cgcggtcggg aaccttcaca ccgatatagt
    12361 ctccgggaac ggccttttcc actttctggt tattgacctc catggactct atggtatcgg
    12421 ttatgtcggt ggtatgaccg gtaatcttaa tcgtgtcgcc gatctttagt tcgccgctga
    12481 cgatctttac cgccgccacc gagggtttgg caaaaaattt gaccacttcg ccgatttttt
    12541 cttccgccat aatcagacct cctttcgtcg cataattcgg ggattaagat gcggcgaaac
    12601 tctatccttt tttcccgaaa aagacaagga taaataaaaa gggccgggat tagaccggcc
    12661 ctttgggttc accgtgatac agccgctgct tctcaggcct ttttggccgc gcccgccttt
    12721 ttcttgcttt tctccttgtc cgtgtcttta acgaattcca ccagggaaac cggggcgtta
    12781 tcgccgatcc ggaaaccaag cttgacgatg cgggtatacc cccccgcccg ttcccggtaa
    12841 cgcaccgaca actcctcgaa gagctttttc accgtctttg cactgcgaac ataccccagg
    12901 gcctgccggc gggcatgcag gctgcctctc ttgcccaggg tcaccatttt ttccgccacc
    12961 cttttcagtt ccttcgcctt ggcatccgtg gtctgtatcg attcatgatc gataaaggat
    13021 gtgaccatgt tcctcaacat ggcctttctg tggctggttg ttcttccgag tttacagtcc
    13081 gcctttccgt gacgcatagt tccttgtcct ctctatttgc tctccccgag ctgccctgcc
    13141 tgcccggggc aacgtcattc ccggttatcc tccttagagt tccaggggtg aaaatccagt
    13201 ttcatgccga aacccagtcc ctgttcggca agaatctctt tgatttcatt gagtgatttt
    13261 cttccaaagt tcttggtttt caacatttcc gcttccgttt tctggaccag ttcgccgatc
    13321 aggttgatcc cggcgttttt cagacagttc gccgagcgca ccgacaattc cagatcgtcc
    13381 acgtggcgga ggagctgctc gttgaggttg tccttttcct cgatctcggg ctgctcggac
    13441 tcctcttcct gttcgctgaa attgatgaag atatccaact gttcctttag gatcttcgag
    13501 gcaaacgcca tggcatcctc gggcagaacg ctcccgtccg tccagacctc aagaacgagg
    13561 cggtcataat cggcgatctg gcccacccgg gcatgggtca cggtgtaatt caccttcttg
    13621 atgggtgaaa atacggcgtc gatattgatg gtcccttcgg gctgatcgta atctttctcc
    13681 tttttcgcct gtacataccc acgtcctagc ttggcgacca tttccgcctt cagattcgtc
    13741 tttccggaaa gggtggccag atagtggtcg ggattgagga tctcgacggt gctatcatgg
    13801 gcaatgtcgt cggccgtgac gattccctcc cgggagatgt ccagatttag catccggggg
    13861 ccatcctgat ggagcatcac ccggacgccc ttgaggttga ggatgatatc cgtcacatcc
    13921 tccttaaccc cggggatggt ggaaaattca tgaagaacgc cgtcaaaacg taccgatacg
    13981 atggccgccc cctggattga agacagcagg gtcctccgca gggcattgcc cagcgtgacg
    14041 cccatgcccc tttccagggg ttgacaggtg aactctccat aatatcgtgt atgggtagtc
    14101 tcgtcgattt ctatccgctt tggtcgtatt aagccgcgcc agtttcgctg cattgtctta
    14161 gtccttattg tccccggcct cggttaccgc gccgggaggt tatgttcatt ttgaataaag
    14221 ctctacaacc agctgttcct gaaccggcat cgtcaactcg tcgcgggtgg gaagcatctt
    14281 tacggcgcct ttgaaactat ccttgtccag agcaagccac tgcggcactc ctcgccgggc
    14341 aaccgactcc agggcctcga ctatccgggc tatctttctg cttccctccc ggacttcgat
    14401 ctcatccccc accttgacca tgtaggaggg aatattgacc tttttgccgt tgacggaaaa
    14461 atggttatgc cggaccagct gccgggcttc ggtacgggaa ttggcgaaac ccatgcggaa
    14521 gaccatgttg tccagtcttc gctccagaag aatcagcaga ttggttcccg taatcccctt
    14581 gatccggtcg gccttttcaa aataaccgtg gaactgcttt tcaagaaggc cgtacatcct
    14641 gcgcaatttc tgcttggccc gcaactgcac gcaataatct gtgggctttc gtctcatctg
    14701 gccgtggtct cccggggcgt attcccggcg ttcgaaggcg catttttcgc tgtagcagcg
    14761 atcgcccttc aaaaacaact tcaggccttc ccggcggcat aacctgcata cggattctct
    14821 ataccttgcc aaagttcctc cctgtcaaat catattctgc aataaagaca aaaacaaatc
    14881 ctataccctt ctccgtttcg ggggccggca tccattatga ggaatcggag taacgtcacg
    14941 gattagcgtg atgttgattc cggcaacctg caaggcccgc agggccgatt cccgacccga
    15001 cccgggtccc ttgacataaa cctgaacgct tctcatcccg tgctctttcg ccttcttcac
    15061 cgcatcttcc gccgccatct gggcggcgaa gggtgtactt ttgcgggagc ccttgaatcc
    15121 ctgaatgccc gaagacgacc aggagacaac attaccgctg agatccgtaa tggtcacgat
    15181 ggtattgttg aatgtagact ggatgtgggc aattccctcg ggaatattct ttttttcctt
    15241 tttcttgcct gtttttctcg ccggcctcgc cattgttcct ccccgttact tcttctttcc
    15301 tgctatcgct cgcctcgggc cctttcgtgt ccgggcgttg gtgtggctcc gctgtccccg
    15361 gacggggagt cctttgcggt gccgcagtcc ccggtaggta cccagatcca tcaatcgctt
    15421 caagtccatg gacacttccc gccgcagatc gccttccacc ttgtggtcct tgtcgattat
    15481 attacggatg gctgtgatct ccgcgtcggc aagattatcc gtcttcacgt tggcgtccac
    15541 ccccgccttc tcgagaatct tctgtgcggt cgttctgccg ataccataaa tataagtcaa
    15601 ggctatttcc atccttttgt tcttcggtaa atccactcct gcaattcttg ccacgcgtct
    15661 atcctcctga cctcatcgac ggttttcagc cttgtcgttg cttgtgtttg ggattctcac
    15721 agatcacccg aagaatgccc tttctcttga cgatcttgca cttgtcgcag atctttttca
    15781 cggaagccct tactttcacg atcttcactc ctgtctcgtc cggtcaggcg gtccgccgcc
    15841 tgccgtctat ttcgtccggt agatgatccg cccccgggtc aaatcatagg gggacaactc
    15901 cacggtaact ttgtctccgg ggaggatctt gatgaagtgc atcctcatct tcccggagat
    15961 gtgggctaga acccggtggc cgttttccag ttccacccgg aacatggcgt tgggcagggg
    16021 ctcgatcacc tttccttcaa cctcgattgc ctcttctttc accataaaag tgatccctga
    16081 tatcagcgga actgatcaat gttcatgatt cggtctttga tccttttcga tcactgaagc
    16141 cggctcaaga tttccggacc gttttccgta atggcgatgg tatgttcaaa atgggccgaa
    16201 agcttcccgt cggccgtcac cgccgtccag ccgtcgggtc gcatctttat cttgtatgat
    16261 ccgacattga ccatgggctc aatcgccagg accatccccg ccttcagttt tattcctctg
    16321 ccctgcacgc cgtaattggg gatctgcggt tcttcatgca ggttccggcc gatgccgtgc
    16381 cctacaaaat cccttaccac cgagaagccg gccgtctcga cgtgaccctg gaccgccgcg
    16441 gagatgtccc ccagccggtt gccgacccgc gcctccctaa tggccaggac caaggattct
    16501 tcggtcgcct ggaggagccg caccgcctcc ggagagatcg ttcccactgg aaccgttaag
    16561 gcggcatcac cataatatcc tttataacag gcgccaaaat cgagaccgat gatatcgccg
    16621 tccaccagca ccctttccga aggaaggccg tggatgatct cgtcattcac cgaggcgcac
    16681 agggaaaagg gatagccccg atagcccttg aaggctggtt tggccccttt tttcagtacc
    16741 agttcctcgg agaggcgatc caactgacgg gtggtaattc cttcttttac cttttctttc
    16801 aacgcgacca gcacttccgc gacgatggcg ttgctggctc tgatcttgtc tatctcgctt
    16861 cgctgcttta gaatgaccat ggaaaagagc tcacccactt gtcgtgatgg ttttttcacc
    16921 gccgcttggc gatctttccc ttcttcatga atccttcgta ctgcctggtc agcagatggg
    16981 tttcgatctg cccggccgta tcgagcgcca cacccaccac gatgaggagc gccgtacccc
    17041 cgaaataaaa aggtacgttg aaatacgaga tcaaaatact tggcagcaca cataccgccg
    17101 atacgtagat ggcgccgctg aatgtgaggc gcgtcaggac gtcatcgata taatccgccg
    17161 tctttttgcc cggtctgatt cccggtacat accccccgta cttcttcata ttgtccgcca
    17221 catccacggg gttgaaagtc accgcggtat aaaaatagca aaagaagaca atgaaagcga
    17281 tatagagtaa ctcataaacc atccgcccgg ggaccagcat atccgccacc tgcttcatcc
    17341 acggatgagg gatgaaattg gctatggttg ccggaaacat gatgatggac gatgcgaaaa
    17401 tcggcggaat gacgcctgcg gtattgacct tcaacggcaa atgcgtggac tgcccaccat
    17461 acactttcct accgacgacc cgtttggcgt actgcaccgg gatccggcgc tgggcgcttt
    17521 ccacatatac gatcgtaccc accacggcaa ccatcatgat aataaggatt acgatgaaaa
    17581 agatccccat ttcccctgcc gaaagcagtt ggaaagtgct gataatggcc tcgggacctc
    17641 ggcagacgat cccggcgaag atgatcaaag agatgccgtt gccgatcccc ttctccgtta
    17701 tctgttcacc cagccacata atgaaggcgg taccggctgt cagggtgatc atggtcatca
    17761 aacggaagcc ccaacccgtt tccaggacga tggaagcccc cgtgggtccc gccatgtttt
    17821 ccagcccgat ggcgatgccg aagccctgga taatgctcag gactaccgta ccatagcggg
    17881 tgtactgggt gattttcctc cgccccgctt ccccttcctt ggaaagtttt tccaggtgcg
    17941 gaatggcgat cgtgagcaat tgcaggatga tcgacgcact gatgtagggc atgatcccca
    18001 gagcgaatac ggacagattg ctcaaggcgc ccccggcaaa catgtcaaag agccccagca
    18061 gcgatccctt ggcttgctcg aaataagctg ccagggccgc cgcatcgata cctggcgtcg
    18121 gcacatgggc gccgatgcga tacacgatca ataagccgaa agtgaagaat atccttttct
    18181 gcagttccgg aattttggat atgttgccaa gtccaccgat caattcagac aacctcgaca
    18241 acggaaccgc cggcggcttc tatctttgtt ctcgccccct tgctcaacgc atcaagtttc
    18301 acggacagtg ggtattgaat ttcccctttg cccaacagtt tgactccatc gccccgcttc
    18361 ttgatcaatc ccctggccat caaggcgtcg gcatcaacca cgctgcgctc cggaaaaaca
    18421 tttagctgtc ccaggttgat cgtaattatt tcccttcgga agggattgcg gaaaccacgc
    18481 ttgggcagcc gccgggccat gggcatctgt ccgccttcaa aacccggctt ggcaccgccg
    18541 ccggagcggg ctttctggcc tttgtgaccc ttgccggctg ttccgccgtg ggcgccgccg
    18601 cgcccgatcc gtttgcattt cttcctagaa ccagccggcg ccttcagtgt ctttaaatcc
    18661 atatatcgct atgcctccac ttcttccatg gaaaccagat gacatacctt gttgaccatg
    18721 ccgcgaacct ccggcgtatc ggccaggatg accgtcttgt tcatcttcat gagccccaga
    18781 cccctaagga cctggcgatg tttttccggt ctgccgatat aactccggat caaagtgatt
    18841 ttcaatttgc gtcccacgag acttgacctc attaatagtt tactgtgcca aagacgtctg
    18901 gccgcggagc cgggcgattt ccgccggttc cttcaggcgg gacagacctt cgatggtcgc
    18961 cttcacaagg ttgtggggat tgtgggatcc tagacacttc gtcagaatgt tgtgcacccc
    19021 cgccgcctcc aatacagcgc gcacggcccc gccggcaatc agtcccgtac cttccgaggc
    19081 gggtttcagc aggacccggc ccgctccgta gctaccgatc acctcatagg gaatggtgtg
    19141 gttggcaatg gcgaccttcc tcatgttttt ctttgcctgc tccatcccct tgcggatagc
    19201 ttcggggact tcatgggcct tgcccagccc ggcgcccacg gaaccctgtc cgtcacccac
    19261 caccacgatg gcactgaaac ggaatctccg gccccctttg acgaccttgg cggttctgtt
    19321 gatcgtaatg accctgtcta tcagttcaag ctcttccatc ataattttat ctcatcgccc
    19381 ctcttgtttt ggtcatcaga atttcaaacc gccttcccgg gccgcgtcag caagggcctt
    19441 gatccggccg tgataaagga atcgacctcg atcgaaaacg gcttcctcga tcccggcgga
    19501 tatcaatcgc ttggccacca gtttacccac ctccttggca gcgtccgatt ttttcttgcc
    19561 gccgagttct ccccgcagct ccgggctcag ggtcgaagcc tcggcaatgg tccggcccag
    19621 gtcatcagcg atggcctgaa catagatatg ccgcgccgtt ctgaacaccg ccaggcgggg
    19681 ccggatttgg gtaccggata cctttccccg cacccgggct tttctcttct ctctgatttt
    19741 ttcaaccttc gctgccaatt cacctacccc ttaaaattat gaatgtgcgg caaccgctgc
    19801 cgtttatttc gttcctacgg acttgcccac tttccgacgc acctgttcgc cggcgtatct
    19861 gatgcctttg cccttgtatg gttccggttt tctcaggtcc cgtatctccg aggccacctt
    19921 gcccaccaga tacttgtcga taccggaaac gagtatattg acctgctttt cgatcttgac
    19981 ggacacccct tgcggcacta catattcccg cggggcggaa aagccgacga caagcttcag
    20041 gacattgttg ttcaattccg ctcgatagcc taccccggat atttccaggc ccttttcaaa
    20101 gcccttgctt accccgatca ccatattgtt caccagggtc ctcgccagcc catgggccga
    20161 tcttcccaac cggttctctt ccagacgctg gacggaaacg gcattttccc catgcatgaa
    20221 ttcgatcccc aggggtaaaa ccgcggacag ttccccttta ggaccggtca ccttaaccag
    20281 cctgccttcc tgggccacct tgacgccgac aggaatttcg atggtctttt tcccgattct
    20341 cgacatattt ctatctcccg accctattca aacacgccct gggaacgctt accacacctg
    20401 acagatgatt tccccgccca cattgagatc ccgtgcttcg cggtccgtca tcaggccctt
    20461 ggaagtggac atgatggcaa tcccgtagcc gttcaacacc ttgggaatgt ccgcgctgtg
    20521 gacataaacc ctccgcccgg gccgactgac ccgctgaatg tccgtaagga catagtttct
    20581 ggaatctgga taaaccagct cgatccgcaa gacttcacgt ttatcttttt cctttttcag
    20641 ttcgtagccg gagatgtatc cggccttctt cagaacctcg gcgacattcc tgttcatccg
    20701 ggaggccagg acatccaccg tcttgaagtg catgcggttg gcgttccgga ttctggtcaa
    20761 catatcggca attggatcgg tcattcccat gaataaaact ccttatcttg atctaccaac
    20821 tcgctttgat caccccgggt atctcccctt tggcggcaag attcctcagg cagatcctgc
    20881 acatatcgaa cttgcgataa taagcccgag gacggccgca cagcggacag cggttgtact
    20941 gccggaccga aaatttgatt tctctttttg cttttgcgat cagggatttc tttgccactt
    21001 atatcttcct cccgccgttg cccggcttaa ttcctgaaag gcatacccat cagcctcaac
    21061 aattccctcg cctcatcgtc cgtcttcgcc gtagtgacga tgaccaggtt catccctctg
    21121 atcttgtcga tcttgtcgta ctcgatctcg ggaaagatga tctgctcctt tagccccagg
    21181 gcgaaatttc cccggccgtc aaaggatttt ggggcaatac cgcggaagtc ccttacccgg
    21241 ggcaggttga tattgaccag gcgatccagg aattcataca tccgctccct ccggagagtg
    21301 acccggcacc cgatggacat tccctggcgc agcttgaagg tggcgatgga ttttctcgcc
    21361 ttggtgatca ccggtttttg cccggtaatc gccgctatct ccgcggcggc cgtatccagg
    21421 accttggcat tctggatggc ctcgcccagg cccatgttga gcacgatctt ctccagtttc
    21481 ggcacttcca tgcgattctt gtagccgaac ttgcctatca atgcggggac tacttctttt
    21541 tggtaataat ctttgagtct tgccatatcg atcgccttat gcgtctatta tctcctgaca
    21601 tttcttacag atacggacct tttttccttc ctcgaggatt ttgttcccga gccggacccc
    21661 cttttcgcac ttgctgcaga agtaggcgac attggtgatg tgaatggggc cctccttctc
    21721 cacgatgccg cccttgccct tggcatcggg ccgctggtgc cgcttcacaa agttgagctt
    21781 ttccacgatc acccggtctt tgtccctcat gattctgatt atcttgccgg ttttaccctt
    21841 ttccctgccg gcggtaacct gtaccgtatc tccttttttt aactgctttc catgcatcgt
    21901 cttgatcctt gtttcgatgt ccctcgtcct gttcagagga cttcaggagc gagtgaaatt
    21961 attttcataa actgtttcgc ccgcagttct ctcgccaccg ggccgaagat gcgcgtcccg
    22021 atgggctcca tctgattggt gatcaggacg gcggaattat cgtcaaactt gagataggaa
    22081 ccgtcctgcc ggcgcacctc tttaaccgtg cggacaataa cggccttcat gacatcgccc
    22141 ttcttcacct tcgaattggg gatggcttcc ttcacagata caacaataac gtctcctatg
    22201 ctggcatagc gccgcttcga accccccagc accttgatgc aggcaacctt cttggctccc
    22261 gagttgtccg caacactaag tatggtttgc atctgaatca tcttaccgta ccgccttcac
    22321 taatttccct gtgctatttt gcccgttcta ggatggaccg catccgccag cgcttatcct
    22381 tgctcaaagg ccgggattcg acgatgacca cccggtcgcc cacccggcac tcgttggctt
    22441 cgtcgtgggc cttgtattta gcgaattgtc gaatatattt gtgaaacacc gaatgtttga
    22501 ccaggcgctc cacgcgaacc acgatggtct tgtccatctt gttgctcacc acgacgccgc
    22561 tgatcgttct tcggtttccg cggacctcca tgattatcga ccctccttct gcttcaggac
    22621 cgtattgatg cgggcaatgt cttttctcac cttccccagt gccgcggtgg aatccaactg
    22681 ccgggaagaa tgacgaatct tcaaacggaa cagctcttca tccagctccg cctttttctg
    22741 tttcaattcg tcgctctgta gctcccttaa ctccttagtt ttcatcggcc gcctccctgg
    22801 tcagaaattt cacgctgatg gggagtttat gagacgcaag gcggaaggcc tctttggcaa
    22861 tatccggggt tacgccgagc atttcataca ggaccatgcc tggtttgacg acggccaccc
    22921 actcttccgg ggctcctttc ccctttccca tcctcgtttc cgccggcttt ttcgttacgg
    22981 acttttgggg gaacacccgg atccagatct ttccgccccg ctttacatgt ctggtcatcg
    23041 ccacccgggc ggattcaatc tgccgggccg tcacccatcc gcactccgtg gcctgcaggc
    23101 cgaactcgcc aaagacgatg gcgttgcccc gcatggccgg cccagtcatg cgtccccgct
    23161 gcggttttct gtattttacc ctcttcggca tcaacatggt tcaaaaaccc ctatatatct
    23221 tctgtaaact gctgctatcg ttcgcgctct ctttttgttc ccaggatttc tcccaggaag
    23281 atcaacacct tcaccccgat ttttccgtac gcggtccgcg cctcggcaaa accccagtcg
    23341 atatcggccc ggagcgtatg cagggggaca cgaccctccc ggtaccactc ggccctggcc
    23401 atttcggcac cgccgagccg gcctgaacag ttgacgcgaa tccccttcgc tccgaacttg
    23461 atggcggaag taacgccctt cctcattgcc cggcggaaag cgatccgccg ttccaattgc
    23521 agggcaacgt tttcggccac aagctgggcg tcgatttccg gtttccgcac ttcgagaata
    23581 ttgatgatca gctcgctact gctcaccttt tcaagatcct tcttcagttt ctcgatttcg
    23641 gaacccttct tgccgatgat gattcccgga cgggcggcat agatgttcac cttcgcttta
    23701 ttggcggccc tttcgatctc aattctcgaa ataccggcat gatagagttt ctgcttgaga
    23761 taccggcgga tgcggagatc ctcgtggagg agctcgctgt agtttttctc agaaaaccaa
    23821 agcgacttcc atgttttaac gccgccaagc ctcaagccga tcggatttac cttctgcccc
    23881 aacctttcac ctccttgcga tgtcgctatg cctcgtccaa cacaacggta atatgactca
    23941 tccttttctt gatcgccgcc gcacggccct gggccctggc ccgccagcgc ttcagcgccg
    24001 gcccttggtc aacgtaaaca cccttcacga agaggatgtt ttcatcgata ttgggattct
    24061 gcttggcgtt ggccagggcg gaatgtaaca ccttgcccaa cagaaacgcc ccgtacttgg
    24121 tggtgtagga cagtatcttt tccgcttctc cagccttctt tccgcggatg agatccgcca
    24181 cgagacgcgc cttctgcggt gatatgcgaa tatacttggc aacagccctt gcttccataa
    24241 acttacccca cgcccgccgc ccttaaggcg gctggtatta tcttctcact ttcgattttc
    24301 tatccccggc atggctgaaa aatgtccggg tcggagcgaa ttcaccgagt ttgtgtccca
    24361 ccatgttttc ggtcacaaaa acggggatga acttcttgcc gttgtgaacg gcgaaggtat
    24421 gaccgatgag atccggggtt attgtcgacc gccgtgacca cgttttgatg accgtcttgc
    24481 tccccgccgc ctctgcctta ctcaccttgt ccagcagttt gggctcgata aatggaccct
    24541 tgctaaccga ccttgccacc ttctaacctc ttctctttac aatgtatttg tctgtgctct
    24601 tattcgtacg cgtcttatgt cccttggtgg gaatccccca gggggtacac ggatgccggc
    24661 cgcccgacga ttttccttcg cctcccccca tgggatgatc aatcggattc atagccaccc
    24721 cgcgcacctt gggacgcctg ccgagccagc gggtcctccc cgccttgccg atattgatat
    24781 tttcatgctc gatattgccg agctggccga tggtggcccg gcagtcgaga aagatctttc
    24841 taacctctcc ggagggaaga cgaacctggg cgtaaccccc ttccttggcc atcaactgac
    24901 cgtaagaccc ggcactgcgg atgatctggc cgccccggcc tactttcaac tctacgttgt
    24961 ggatcagggt gcccaagggg atgttcttca ggggaatgga gtttcccgct ttgatatccg
    25021 cccgctcgcc gctgatgacg acatcgccga cggcgatctt gtccggggcg aggatatagc
    25081 gtttctcccc atccacatag ttcaataggg cgatcctggc cgaacggttg ggatcgtatt
    25141 cgatggatgc cacccgtgcg gggatgtcga gcttgtcccg gcgaaagtcg atgatgcggt
    25201 accgccgctt gtgaccaccg ccgatatgcc ggctggtcat ccgtccattg gcgttccgcc
    25261 cgccgctttt gcgcagggga cgcaacagac tcttttccgg gatggaagcg gtgatctcct
    25321 caaaatcgga acaggtctga aacctcctcc ccggcgatgt gggtttatat gtcttgattg
    25381 ccataatccg tgtccttaaa ccttaatcac acaccaattt aaacaccttc gaatatttcg
    25441 atgcggcttc ccggcgccag ggtcaccacc gccttcttcc aggacggctt ctcaccgacg
    25501 atccggccga tccgcttttt ctttcccggc acattcatca ccgccacatc caagactttc
    25561 accttgaaga gtttctccac ggcggcgccg atttcgatct tgttggcccg gcgatcaacg
    25621 gcaaaaaagt acttgttcgc ctcgtccttg gcgatggtgg ttttttccgt aatcagggtt
    25681 ttttttataa tcttgtataa ttccattatg atcgcaacgc cccctcgatg tttttgacgg
    25741 acggcgccag caaaaccaca tgctcatgat tcaagaggtc gtaaacgttg atgccttccg
    25801 agcgaatcat ttttatttcc tgtaggtttc gcgaagattt ttccaaaatg ggattggatt
    25861 tgtcgatgac caccaaggcc tttttccaac cgaaacgatc gactacttcc ttgaacttct
    25921 tggtcttgac ttcggccaag gggaagtcac tgaggatgat catccgctct tctttgaatt
    25981 tctggctgag cgccgatacc agcgcgagct tccgaacctt tcttggcact ttgaaggcat
    26041 agtcccgggg atggggtccg aataccgttc ctccccctcg ccagataggc gaccgcgtgg
    26101 ttcccgctct ggctcgcccc gttcctttct ggcgccaagg ctttttcccg ccgccgctta
    26161 tgtcccgccg cgtcttcgtt gccgccgtgc ctaggcgccg cgtcacgagt tgcatcctta
    26221 ccacctcgta catcgcggcc tcgttcacgg cggcaccaaa cacctgatcg ctcagttcga
    26281 tctccgctac tttattgttt tcaatatcgt atacagccgc tactggcatc ttgtccaccc
    26341 tgtatcgcta tttctcaacc gttgttttct tgactgccga cctgatcaga acgatcccct
    26401 gcttgcctcc ggggacggcc cccttcacca gcagggcgtt gtcttccggc cgtacgagga
    26461 gcaccttgat gttctggatg gtgttccgtt caccccccat tcgcccggga agcttctttc
    26521 ctttgaagac ccgcgaaggt tcggcgctcg ccccgatgga accgggggcc cggtgaaaca
    26581 tcgagccgtg ggtagcccga ccgccgccga aaccatgcct cttcactccg ccagcaaaac
    26641 ccttgccctt ggaggtaccg gtaatatcca cataatctcc ggctttgaag atgtcggcac
    26701 acagttcctc tcccggctta tagccgccgg cctccaggcg aaattcccgg agcgtccgga
    26761 agaatccttt gtccgccttg cggaaatgac cctggagagg cttagtcacg tgtttctgtt
    26821 tgatccggcc atatcccaac tgtactgctt cgtaaccatc cttttccgtg gtcttcctct
    26881 gaacgaccac tgaaggttcg acctcaatga ccgtcgccgg cacggcaccg ccatcttcgg
    26941 taaagatctg ggtcattccc agttttctgc ctatcagtcc ctttatcatg tcggttctct
    27001 aatcgaaaaa aatcaggaga tggaaagtct gactctgttc catcccctgt catatacaac
    27061 ctatagtttg atctccacat ccaccccggc cgacaggtca agcttcatca gggcgtcgac
    27121 cgtggactgg gtgggttcaa tgatgtctat caaacgcttg tgcgtcctga tctcaagctg
    27181 ttcccgggat ttcttgtcta cgtggggcga cctgttgacg caaaatctat tgatttccgt
    27241 aggcagggga atgggcccgg caacacgggc ccctgtgcgc ttggccgttt cgacgatatc
    27301 ctccaccgat cggtccagca gcttgtagtc gtaggctttg agacgaatcc ttattctctg
    27361 gtctttcatg gtcgcgccgg tttcctcttt gttattcaat taccttgctg acaacaccag
    27421 ccccgaccgt ccggccaccc tcgcggatgg cgaaacggag ctgctcctcc atggcgatgg
    27481 gggtaattag ctgaatttcc atttccacat tgtctcccgg catgaccatc tccaccccct
    27541 ctggcagcag ggccaccccg gtaacgtcgg tggtccggaa atagaactgg ggccgataac
    27601 cggtaaagaa gggtgtatga cggccacctt cctctttggt cagtacatag acctgggcct
    27661 tgaatttggt gtggggggta atggaaccgg gtttggcaac cacctgtccc ctttccactt
    27721 cttcccgctt tgtgccccgg agcaggacgc cgatgtcgtc gccggcccgg ccttcatcca
    27781 gggtcttgcg gaacatttcg acgccggtgc agacggtctt gaaggtggga cgaatcccaa
    27841 cgatttctac ttcctcgccg gttttgatga tccccctgtc cacccggccc gtgaccacgg
    27901 tgccgcggcc ggagatgctg aagacgtccc cgatgggcat gaggaaaggt ttgtccagaa
    27961 cgcgttgcgg ttcggggata tagttgtcca ccgcattcat gagttcccag atggatttgg
    28021 cttcttcgga attgggatca tcggtctcca aggccttcag ggcggagccg cggatgatgg
    28081 ggatatcgtc gccggggaac tggtactcct tgagaagttc ccggagctcc agttcgacca
    28141 ggtccagaag ctccggatca tcgacgagat cgaccttgtt catatatacc accacatagg
    28201 gcacctggac ctggcgggcc aggaggatgt gctcgcgggt ctggggcatg ggaccgtcgg
    28261 aggccgccac gacgaggatt gtgccgtcca tgtgggcggc gccggagatc atgttcttga
    28321 tgtagtcggc atggccgggg caatcgacgt gggcgtagtg ccgtttgtcc gtctcgtact
    28381 ccacatgggt gatattgatc gtaacacctc gctccttttc ctctggggca ttgtcgatgg
    28441 agtcaaagga cctgaactcg gcgaaacctt tcttggacaa gtgcttggta atcgctgccg
    28501 tcaaggtcgt cttcccgtga tccacgtgac cgatggtacc gatgttcaga tgcggctttg
    28561 tcctttcaaa ttttttcttg gccatctcta cttacctcct tcgtctgttg aaaaaactct
    28621 tatgtatttg tgaccctttg ctcctgcaat gacctgtctt gaatttctct aaaaccgata
    28681 gtgggcaaag gccttgttgg cctccgccat tttgtgcaca gcttcccgct ttttgatcgc
    28741 cgcgcctcta ttgttggcgg cgtcgatgag ctccgccgcg agtttttcct tcattgtctt
    28801 ttctgcccgt tccttggcat tggtgatcag ccagcggatc gccagggcca ttcgccgcga
    28861 cggttggacc tccgtgggca cctggtaggt ggaaccgccc acccggcgcg atttgacctc
    28921 tatcaccggt ttcacattgc tgagggcctt ttcgaataca tccaccggcg gctccttgac
    28981 ccgtttttct atgatgtcaa acgccccgta gagaattgat tccgcggtgc ttttctttcc
    29041 ctgcttcaaa agattgttga cgaactgggc caccatttta ttattgtact tgggatcagg
    29101 tataatctcc ctttttgtta cctctcgtct tctcggcatg atgatctatc cccgcctaat
    29161 tcggtttctt ggccccgtat ttcgatcggc cctgttttct tccctggacg cccacggcat
    29221 cgagggtgcc ccgcacgata tgataacgca cccccggcag atccttcact ctcccgcccc
    29281 ggaccaggac tacggaatgc tcctgaagat tgtgccccac cccgggtata taggacgtta
    29341 cttcgtaccc gctggttagt cgcacccggg cgaccttgcg caacgccgag ttgggtttct
    29401 tgggggtcga tgtatagaca cggacacaca ctccgcgccg ctgcggcgac ccgaccagcg
    29461 ccggggactt agtctttcct tctattttag tcctgccttt gcggactaac tggtttatag
    29521 tcggcatgtc tcctcccgaa agctcttccc gtccgcacgg acaggtacgg caaagcttgg
    29581 cttactacca ataatgagat tacatgtcaa gtattttctc atcactattt ccctccagct
    29641 cttgtgtttc ctgctcttcc gcacggtcgg ccacctcgtc ggccagggtt ttgatcccga
    29701 gtttccggta catgggcaga ccggtgccgg cgggaatcag acgccccatg ataacgttct
    29761 ctttcaggcc ccggagataa tccgttttcc ccgccagact cgcctcggtg aggacgcggg
    29821 tggtctcctg gaaagaggct gcagaaatga aactctgggt ggacagggag gccttggtaa
    29881 tgcccaggag gatgggtttg cctaccgccg gtctcccgcc ttccgaggcc acgcggtcat
    29941 tctcctcctg aaaacggtga cgctccacct gctcgtcggc gataaacacc gtatcgccgg
    30001 gatcgacgat tttgacccgc cgcagcatct gacggacgat gacttccatg tgcttgtcgt
    30061 tgatcttcac cccctggagg cgatagacct cctgcacctc atccacgaga taacgggcca
    30121 gttccttctc gcccttgatg gacagaaggt catgggggtt atgaatcccg tccatgagcg
    30181 gttccccttc ctgaacccgg tccccttcct ggacgctcac atgttttccc ttggggatca
    30241 ggtattcctt ctcctcgccg ctgtcggggg taatgatgac cttgcgcttc cccttcgcgt
    30301 ccttgccgta ggaaacgacc cctcttattt cactgatgac ggcgtattcc ttgggtttcc
    30361 ttgcctcgaa aagttcggca acccggggca gaccaccggt gatatccttg gtctttgtgg
    30421 tttcccgggg gattttggca atgatgtcgc cggcgctgac ctgatcgcct tccgagacca
    30481 caatgtttgc tcccacgggc agcagatgcc gggccaccat attcgtgccg ggtaccttcg
    30541 ccgtccggcc cttcttgtcc ttgatggaaa cccggggccg cacgtccgtc cccttggatt
    30601 cgatgacaac cttgcgggaa aggccggtga cttcgtcgac ctgctcctgc atggtcttgc
    30661 cctcgatgat gtccccgtac ttgatcgtgc cgtccacctc ggccaggatg ggaatcgaga
    30721 agggatccca ctccgcgatg agcgtggcct gtcgttcctg tcctgttttg gggtcctttc
    30781 ccggaccgta cacggtatcc ccttcattta cctttagatg ggcgccgtaa ggcaccgggt
    30841 atttgccccg gttgcgcccc tgctcatcca gcacgtggat ctcgccgttg cggttcatga
    30901 cgaccagatc gaccatgccg ccggcctgga tcttgacagt ggaaagattt acaaacttga
    30961 cgacgccgtc atgacgggcc tggagggttg aatgctcctc gaacttggcg gtgccgccga
    31021 tgtggaaggt tctcatcgtt aattgggtcc ccggctcccc gatggactgc gccgcaatga
    31081 tacccaccgc ttctccaatg ttgaccaaat ggccgtgggc cagatcgcgt ccgtaacaca
    31141 tggtgcagac gccgtgtttg gacttgcagg taagaaccga acggatgctc acccgctcga
    31201 tgcccgcctt gtcgatccgc tccgccagga cctcgtctat ctcctgattg gcttccacta
    31261 aagtctcccc ggtgaaggga tccacgatct cctccagggc gacccggccc aggacgcgct
    31321 ccccggccgt ctcgataatt tctccacctt ccatgagcgc cgagacgtag atgccgtcga
    31381 tagtcccaca atcctcttcg gtaatgatgc agtcctgggc cacgtccacg agccgacggg
    31441 tgagataacc cgaattggct gttttaagcg ccgtatcggc caatcccttc cgggcgccat
    31501 gggtagaaat gaagtattgc aaaaccgtga gaccttcacg gaaattagct gtaattggcg
    31561 tctcgatgat ctcccccgaa ggcttggcca tgaggccccg cataccggcc aactggcgaa
    31621 tctgggccgc cgagccccgg gcgccggaat cggccatcat aaagatggga ttgaagctgt
    31681 cggtgcgctt cttttctccg gaaaccatgg tcacctccgt ggaggcgatg ccgcccatca
    31741 tctccccggc gatcctttcc gtggcctggg cccatatgtc aatgactttg ttgtagcgct
    31801 cgccgtcggt aataaggccg tccatatact gtttctgaat cttcaacaca tcccggtcgg
    31861 cccggcttac gatttctttt ttgttggcgg gcacggccat gttgttaatg gcgatggaag
    31921 ccccggagtt ggtggcgtac ttgaagccca aatccttcag gcggtcggcc aggagaaccg
    31981 tcgtcttggc gccgcaggtc cgataagtga aatcgataag gctagcgagt tccttcttgt
    32041 tcatcacctt gttaatgaga tcgaaggcga tgccctcggg gatgacttca tagagcatga
    32101 ttcggcctac ggtcgtttct ttccgttcac cgtcgatacg gacggtgatc cgggcatgaa
    32161 gatcgacgct gccggcgtcg taggcgcttc gcacctcctc gggtccggaa aacaccatgc
    32221 cttcgcccag tacgtccgtc ttggcacgcg tcaggtaata gatcccaagg acaatatcct
    32281 ggctggggat gatgatcggc ttgccgtggg ccggggaaag gatgttgttg gtggacatca
    32341 tcaacacccg ggcctcggtc tgggcctcga tggacaacgg cacatgcacg gccatctggt
    32401 cgccgtcgaa gtcggcgttg aaggcggtgc agaccagggg atggagctgg atcgccttgc
    32461 cctcgatgag gaccggttcg aaagcctgaa ctcctaaccg gtgcagggta ggggcacggt
    32521 tgagcatgat ggggtattcc ctcaccacct cgtccagggc gtcccatacc tcggaagact
    32581 ccctctccac catcttcttc gcgcttttta ccgtggccac ataccccttg tccataaggc
    32641 ggttatatac aaaaggcttg aatagctcca aagccatctg cttgggaagc ccgcactggt
    32701 ggagtcgcag atccggaccc acggtgatga ccgaccggcc cgaataatcg acccgcttgc
    32761 cgaggagatt ctgacggaaa cgtccctgtt tgcccttgag catgtcggaa agggaccgga
    32821 ggggacgctt gttggtaccg gtaatggccc ggccccggcg tccgttgtcg aacagcacat
    32881 ccacggcttc ctggagcatg cgcttttcgt tgcggatgat tatctccggg gcgttgagct
    32941 ccataaggcg tttgagccgg ttgttacggt tgattacccg ccggtagagg tcattgaggt
    33001 cggaggtggc gaaacggccc ccgtccagag gcaccagagg acgaaggtcc gggggaatga
    33061 cggggagcac ggtgaggacc atccactccg gtttgttgcc ggatttcttc aaggcctcca
    33121 ccaccttgag gcgcttgacc aattttttct tttttgtatc cgaaacggcg gttttcagtt
    33181 cctcccggag ttcaccgtcc atctggtcca ggtccagctc ttcgaggagt ttgcgtacgg
    33241 cttccgcgcc gatgcccacc tcgaaggcgt cctcaccgta ttgctccctt agttcatgca
    33301 cctcttcctc ggaaagcagt tcctttttcc gcagggaagt ggtcttggga tccatgacga
    33361 tccaggcctc aaaatagagt actttttcca gttccttgag ggtgaggtca aggacattac
    33421 cgatccggct gggcagactc ttaaggaacc agatatgggc caccggtgtg gccagtttta
    33481 tatggcccat tctttcccgg cggaccttgg attggatgac ttccaccccg catttctcgc
    33541 agatgacccc gcggtgcttc atccgtttgt agcggccgca aaggcattcg taatccttca
    33601 ccggaccgaa gatcttcgca caaaacagac cgtccctttc gggcttgaag gtacgatagt
    33661 tgatcgtttc cggcttcttg acctccccgt tggtgttcgg ccgggccgac caggaaagaa
    33721 tcttttccgg ggaggcgatg gaaatcttta tggcattgaa cctgatgggg tccttcggct
    33781 tttcaaagta ggtgaatacg tcttccacgc atctgctcct taactttctt cctcaattaa
    33841 ttcaacatcc aggcagaggc tctggagttc cttgaccagg acgttgaacg actccggcag
    33901 ccccggttcc agggtgtgtt cccctttcac gatggtctcg tacatcctcg ttctccccgc
    33961 cacatcgtcg gatttaaccg tgagaaattc ctggagggaa taggcggcac cgtaagcctc
    34021 catggcccag acctccatct cgcccagtcg ctggccgccg aactgggcct tgcctcccag
    34081 gggctgctgg gtgaccaagg aataggggcc gatggagcgg gcatggatct tgttgtccac
    34141 caggtgatgg agtttgagca tgtagatcat gccgacggtg atctcctggt cgaagggctc
    34201 acccgtcctc ccgtcgaaaa ggaccgtctg gcctttctcg ggaaggttgg cgagtttcaa
    34261 catctgccgg atctcgtttt catcggcgct gtcaaagacc ggggtggaga aggagatccc
    34321 actttgcaga cgagaaacga atctcttgac atccccctcc gatgccttgt cgataaaaac
    34381 attcgcctcc ggagaggcat aaattgcctt gatttccttt ttgacctgct ccagattctg
    34441 ccgtgtcttc atcagttcgt tgatcttgtc gcccagacct ttggccgccc aaccgagatg
    34501 ggtctccaaa atctggccaa cgttcatccg ggagggaacc cccaggggat tgaggacaat
    34561 atccaccggg gtgccgtcca caaaataggg catatcttct tccggcagga tccgggaaag
    34621 gactcccttg ttcccatggc ggcctgccat cttgtcgccc acggataatt tcctcttgat
    34681 ggctacatag accttaacca tcttgatgac tccggggggc aattcatcgc cggcccggag
    34741 cttttcgatc ttttccttga agtaggcgtc gatcctctcc ttcttccggg tcagattgtc
    34801 gcacagcatc cgaacctgct cttccgtctc ttcatccccg gccagggtga tgtccttcca
    34861 gagatcgaag gggatcttcg ccaggatttc gccatcgatc tcttcgccct tttttaggta
    34921 gatctttttc ttttttccgt ccgcgagctt ggcgccggca gtcttaccga ctagcatttc
    34981 cgtgattctg gcccgtaccg aatcggtgat gatgcgcaac tcgtcattac gatccttctc
    35041 tatggaactc agggcttcat cctcgatttc ctgggaccgg ctgtcctttt ccaccccttt
    35101 gcgggtaaat atcttggcgt cgatcaccgt accttccacc cccgggggaa cccggaggga
    35161 agtgtcgcgc acgtcgctgg ccttctcgcc gaaaatggcc cggagcagct tctcctccga
    35221 ggacagttgc gtctccccct tgggggtgat cttgccgacc agaatgtctc cgggcttcac
    35281 ggatacgccc atgcggacga taccgctttc gtccaggttc ttcagggcct cttcccctac
    35341 attggggatg tctctggtta tctcctcctt gcccagtttc gtgtcccggg acatggtctc
    35401 gaactcttcg atatggatgg aggtatagat gtcctccttg acgacccgct cactcaccag
    35461 aatcgaatct tcgtaattgt acccccccca ggacatgaag gccaccatga cgtttcttcc
    35521 cagggccagt tcaccgttgt ccgtggcagg accgtcggcg atgatgtccc cgcggcgcac
    35581 tcggtcgccc ttgacgacga ttggcttctg attgaagcag gtgtcctggt tggaacgttg
    35641 atatttgacc aggttgtaga tatccacccc gatgtccagg ttgtcatcgc cggtgccgtc
    35701 gcatttgacg acgatgcggg ccgcatccac gctttccacc actccgtccc gccgggcggc
    35761 cactacggcc ccggaatccc gggccacgac cgcctccatg ccggtgccta ctaccgggac
    35821 ggcgggtctc atcaacggca cggcctggcg ctgcatgttg gagcccatga gggcacggtt
    35881 ggcatcgtca tgttccagga agggaatgag cgtggcggcg acgctgacga gctgattagg
    35941 agaaacatcc atgtagttga tctcttccgg ttcgacggta atgaaatcgc cgcccttgcg
    36001 ggcggagatc atctccccga taaaccggcc atccttgtcc agaggacgat ccgcctgggc
    36061 gatgataaaa ttttcctcct cgatggcagt catatagcgg acctcgtcga ggacccgccc
    36121 gtcttttacc aggcgatagg gggtttcgat aaatccgaac tcgttgaccc gggcataggt
    36181 ggaaagcgag acgatgaggc cgatattggg cccctccggt gtttccaccg ggcagatccg
    36241 cccgtaatgg gtgggatgga cgtcccggac ctcgaacccc gccctttccc gggtaaggcc
    36301 gccgggtccc agagcggaaa gtcgccgttt atgggtgatc tccgaaaggg gattggtctg
    36361 gtccataaac tgggacaatt ggctgctccc gaagaactcg ttgaccaccg ccgaaacggg
    36421 tttggcattt acaagatcat ggggcatcat cgtctcgatg tcctgaaggc tcatcttctc
    36481 cttgatggcc ctctccatgc gcacaagccc gattcggtac tggttttcga tcaattcacc
    36541 gactgagcgc acccgccggt ttcccaaatg atcgatatcg tccaccgtac attccccgat
    36601 cccgtttttc aggtcgatga gatatttgac cgccgccaag atatcttcat ttcttagtac
    36661 cgtaacattc gtggggacgt cgaggcggag cttgtagttc atcttggccc gccccacgtc
    36721 ggagagatcg taggtttccg gttcgaagaa gagactccgg aagaaagcct gggccgtttc
    36781 cggggtgggg ggattgctgg ggcggagccg gcggtatatc tcgattatgg catcattcat
    36841 ggtttcgata cgatcagcca gcaaggtctc acggatcgac acattgccca tttcttcatc
    36901 gaggtggatg aaccggacct cccggatgtt cttagcctgg aggagcttca atccctccgg
    36961 ggagagttct tcgttgcagc gcaacaggac ctctcccgtt tccggatcca tgatgtccgt
    37021 tgagaggatc ttgccgacca cgtcagcttc gcttgccgta atgcgcgtaa tcgaagcttc
    37081 ggacatcttt ttcagcagga tcttgttgat cttcctgccc tttttgacaa tcacctcatc
    37141 agacgcgtcc cggatgtctt ccggggcctt ttcgcccagg agggaattat ccacggccat
    37201 aaaaaactga tctccatcca ggaagaccgt ttcgatattg taaaagtaat tcaggataaa
    37261 ttccgtggaa ttccccatgg ccttgaggag aatcgtcacc ggcatcttgc gacggcggtc
    37321 gatccggaca tagaggagat ccttggaatc gaattccaga tccagccagg aaccgcgaat
    37381 ggggatcacc cgggccgaat agatgagctt gccgctggcc agagtcttcc ccttgtcatg
    37441 atcgaagaag attcccggag agcggtggag ctggctgacg atcacccgct ccgtaccgtt
    37501 gacgatgaac gtcccgttct ccgtcatgag agggatctcg ccgaaataga tttcctgctc
    37561 cttgatgtta ctgatctgct tctggttcgt ggacagatcg acgtcgaaaa tcagcagccg
    37621 gacgacgatc ttcagcggcg cggcatagct cattcccttt tgaatgcact ccttgacatc
    37681 atagcgcacc tctccaattt tataactgac gaattctaga gagcatttct tactatagtc
    37741 ggaaatgggg aagacgcttt taaaggcccc ctggaggccg tagttgcccc gctggtcggg
    37801 ggccgtatcc ttctgaagaa acttttcgta ggattttacc tggatatcga tgagattggg
    37861 aatatccaca atcttcttta tccttccgaa atttttacga aactcgccca tatgatcctt
    37921 tcggtttcaa gatccgataa gccgtacctc aataactatt taatctctac ggtaccgccg
    37981 acctcttcga tctttttctt tatatccgcc gcctcatctt tggaaacggc ctccttgata
    38041 ggtttcggca ctccgtccac cagatccttg gcctctttga gccccagtcc ggtaatcgcc
    38101 ctgaccactt taatgacctg aatcttctga tcgccgaatc cggtgaggat ggtgtcgaat
    38161 tccgtctttt cctccgccgg ggcaaccgct tctccaccgg ccgggaccgc ggcaacggct
    38221 accggggcgg cggcggagac accaaagcgc tgttccaatt ccttgaccag ctcggacagt
    38281 tccagaacgg tcatgccttc gataaacttt acgacatctt ccttggtaat ctgacttgcc
    38341 atctttcctt acttccttcc tgttttattt attgtagtga ttcttttttc tccctataag
    38401 catcgaggac ctggaccatc cctctgggga cggcgctcag cacactcacc agtccagtgg
    38461 gaacaccgat caacacggat agcaacctgc caatgagaat ctcacgactc ggcagcagtg
    38521 ccacggcatt gacctgctcc cggttcaaca gctttccctc cagcatggcg gctctgatct
    38581 ccatttcgga gttctttttc gcgaattcca ccagcacctt gaccgtctct accacgtcct
    38641 tgtcagtaag gacgagcgcc aggggaccct tgatatgctc gttcatggcg ctgaaatcag
    38701 ttccctgcga ggcaatgcgc agtagggtgt tcttgacgat ccgcatatcg gtgccggatt
    38761 tccgcagggc tacccgcagg gccgtaatcc gggcgacatc catgcccgaa tagcccgtga
    38821 ggacggcgag cttaaatccc ttcaactttt cgtgaagttc ggcgacaacc tgctcttttt
    38881 cctttcgatc caatcttcaa cctcctttca atcttattgg tccggggcgg cctggggcgg
    38941 ccgtccggac gctacgcaat cgaaagttcc aaaatggcag gtaacaggaa aaaagacgat
    39001 gacaaccaat tatgaacatt aagcttcgct ttcggaacaa taagcttcgc tttcagaatc
    39061 atggtttttc tctcgtctcg gcaggcctgc aattaccgtt taaactcccg tacctgctgt
    39121 ctcagactca atcaatcacg tgttagatat tactgccttt agcggacgtt atatatttct
    39181 cacatcaagg gggtccacct tgaccccggg tcccatcgtg gaggaaaggg agatgctctt
    39241 gagataggta cccttgctgg ccgccggctt caacttgacg atggtttcga tgacggcagc
    39301 gatgttttcc atgagtttgt cggcgccaaa tgagactttc cctaccgggg tatggacaat
    39361 tccggccctt tccacccgga attccacttt gcccgccttc aattcgccaa tggccctggc
    39421 cacatcgaag gtcacggtgc ccaccttggg attgggcatg agcccccggg gccccaggat
    39481 cttgcccagt ttgcccacgc tgcccatcat gtccggagtg gccaccaccc ggtcgaactc
    39541 cagccagccg cctttgatct tctcgatcag gtcatcggcg ccggcataat ccgcaccggc
    39601 ctcctgggct tcccgttcct tgtcgccctt gacgaaggcc aggacccgga ccgtcttgcc
    39661 cagaccgttg ggcagcacga cgctgccccg gaccatctga tcggcatgct ggggattgac
    39721 gcccaaccgc atggcgatct ccaccgtctc gtcgaacttg gcccgtttgg tggcaatgac
    39781 gagttccacc gcttccttga gggcatagcg tttccccggt tccaccttct tctttgcctc
    39841 gataaatgtt ttccctcttg ttgtcatgat caaacctcaa agatcgggga tcaaccctcg
    39901 attgtaattc ccatggagcg cgccgtaccc gtgatgacgc tgatggcgcc ttcaatatct
    39961 accgcgttga gatcgttgaa cttcagttcc gcgatctcct tgatctgctt ggcggtcacc
    40021 gtcccgacct tttccttgtt ggggatcccg gatcccttgg cgatcttcgc cgcctgtttg
    40081 aggagcacgg cggccggggg ggtcttggtg ataaaagtaa aggagcggtc ggcataaaca
    40141 gtaatcacga cgggaataac cgtaccctcc tggctctggg tcatggcatt ataagcctta
    40201 cagaattcca taatgttcac cccatgctga ccgagggccg gcccgatcgg cggggaaggc
    40261 gtggccttgc ctgccttgat ctgcaatttt atgttggcaa ttaccttttt agccatagtg
    40321 ttccacctta tcttcgctga tttagttttg gactacctgg atgaaatcca gctccaccgg
    40381 cgtggatctg ccgaaaatac ttacaatgac tctcaacttc cccttctccg gtttgacctc
    40441 gtcgatgacc ccgtcaaagt tggtaaaggg accgtcgatg atgcggacgt ggtcgccgac
    40501 ctcgaatttg aatttaggtt tcggcttcag ggttccttcg tcgatccgtg tgcgcagcga
    40561 ctccacatcc ttgtccggaa tgggaaaggg tttttccttg ccgccaatga aacccgtcac
    40621 cttgggggtg tctttcacga tatgccaggt atcgtcattc agctccatcc ggaccaggat
    40681 gtaaccggga aagaatttgc gctttgaggt ctttttctcc ccggagacca actcgacgat
    40741 atcctcctcg gggattaaga cgtcggaaaa gaattcctgc ttccctgccg cttcaatacg
    40801 ctcctggaga gacagcttga cccggttttc ataacccgaa taggtgtgga cgacatacca
    40861 tttctgagcc atgttcttat cccagcagta atttaatgag tttggtcaag ccgaaatcga
    40921 cgatgcctag aaaggcggag acaatggccg taacgacgat tactaccgcg gtcgaggcaa
    40981 gggtctgctt cggcgtcggc caggtcactt tcttcagttc gaccttagcc tcggaaagga
    41041 acaccttcac tttgtcaatc caaaccttga tcttatccat caagtaaacc gttcaaaaat
    41101 aaatggcagg ccaggaggga ctcgaacccc cagcatccgg atttggagtc cggcgctc
//
LOCUS       DAOSMF010000006        40544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_1297_length_40544_cov_24.769938, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000006 DAOSMF010000000
VERSION     DAOSMF010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 40544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40544
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1297_length_40544_cov_24.769938"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            383..>1530
                     /locus_tag="PLT63_01855"
     CDS             383..>1530
                     /locus_tag="PLT63_01855"
                     /inference="COORDINATES: protein motif:HMM:NF015882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HOD97495.1"
                     /translation="MIKSTSASYSILMRVELNNRPGMLGKVVSAIGKAGGNMGAVDIV
                     GFKGDCIIRDVVVHAADHDMMEKILAAVRRIAGAAVIDFADRTFMAHMGGKIEVKSKI
                     PLSSRDDLAMAYTPGVARVCEAIHADPERAHDLTIKKNCIAIVSDGTAVLGLGDIGPK
                     AAMPVMEGKALLFKNFGGVDAFPICLDTKIPDEIVETVRRIAPGFGGINLEDISAPRC
                     FEIEARLKEILEIPVFHDDQHGTAVVILAGLLNALKITGKDIGEILAVVNGLGAAGVA
                     AINLLRAAGVRRFIGCDRKGILYRGRKENMNPIKEKLALETNEEQIKGDVFRALKGAD
                     LFLGVSEGNLLHAAHVKKMAKDPIVFAMANPTPEIMPEEAAKYVRIMAT"
     assembly_gap    1531..1630
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1631..1965
                     /locus_tag="PLT63_01860"
     CDS             <1631..1965
                     /locus_tag="PLT63_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HOD97496.1"
                     /translation="TPEIMPEEAAKYVRIMATGRSDYPNQINNVLCFPGLFRGVLDCH
                     AREISRQMLLAAARAIAASVETEQLMEDYIIPSVFNLKVQENVAAAVREVAVTTGLAR
                     DVHEFSLF"
     gene            1973..3205
                     /locus_tag="PLT63_01865"
     CDS             1973..3205
                     /locus_tag="PLT63_01865"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HOD97497.1"
                     /translation="MKKISPSQKIFIGMALGLAVGLLFPHFAPYLEPLSTIFIRLVKT
                     IVVPIIFVSLIVGIAGHGDMKAVGRMGLKAIVYFEIVTTFALCIGLLAVNLLRPGDGV
                     DIGGGREAVVAGKVNSFSDLLVHMFPENVLESAARGDLLQVVVFTVIFSAALIMIGEK
                     KKPIIELCESVTEVMFKYTNIVMRVAPIGVGAALAVVVGSKGISVVVHLLGLIGCLFL
                     SLVVFLIVIIVPVMLILRIPLRKFFAAVKDPAVIAFSTTSSEAALPKAMEAMEAFGVP
                     RRIVSFVIPVGYSFNLDGTTLYLPMAAVFVAQASGIEMSIGQQFFMGLTIMLASKGVA
                     GISRGSMVILAGLLPTFGLPVSAVAVLLSIDAIADMGRTTVNVIGNCLASAVVTKWEG
                     LPLKSGEEASPTAPPGDS"
     gene            3476..4891
                     /gene="aspA"
                     /locus_tag="PLT63_01870"
     CDS             3476..4891
                     /gene="aspA"
                     /locus_tag="PLT63_01870"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF008909.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HOD97498.1"
                     /translation="MTAKRFRIEHDLLGEKEVPADCYYGVQTLRAVENFQISGIRLGR
                     YRFMVRALAFIKKAAARANAKLGLLPEEIARAISQACDEVLAGKYEDQFVVDMIQGGA
                     GTSSNMNANEVIANVALEKLGYEKGCYDIIHPNNHVNMCQSTNDVYPTAIRLALIFKI
                     RELLESMHYLYDAIEDKSIEFRDVIKMGRTQLQDAVPMSLRQEFSAFAVMVGEDIERV
                     KETEKLLREINLGATAIGTGLNAHPEYAPLAVLNLRKFTGVEVETSVNLVEATQDTGV
                     YVQLSGVLKRVAVKLSKICNDLRLLSSGPRCGFNEIRLPAMAPGSTIMPGKVNPVIPE
                     VVNQVAFHVIGNDVTVTFAAEAGQLQLNAMEPIIAFSLFNSITFLNNACLTLADRCIK
                     GITANREQCRLNIERSIGLVTALNPYIGYEKATEVAHEALETGRSVYEIVLEKGYLSQ
                     KDLDSIMSPENMIKPHYFQGG"
     gene            4905..5780
                     /locus_tag="PLT63_01875"
     CDS             4905..5780
                     /locus_tag="PLT63_01875"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HOD97499.1"
                     /translation="MVERHIIMPALAALLLVLTLASGCAGPASDDRSLEITRLKGYFV
                     VGLDDHFPPLSFRLPSVDRTVRYELNLEKKAVEKITVGDELVGFDIDLAKKAAEKLGL
                     RVIFQPVPWDGIIASLEKGEIDMIWSGLSVTPERRERIIFSRPYLDNRQIVMVKSGAA
                     ISGKADLQGKTVGLQLGSSSDTALHAERRIAAHLKELKRYPDDAAAMRELEAGRLDAV
                     VIDEVMGRYLITQQPGVFAVLADDFGREEYAVGFRKNDRALRDAVDRALGEMMRDGAA
                     DAVAKKWFGAAIAEK"
     gene            5928..7208
                     /locus_tag="PLT63_01880"
     CDS             5928..7208
                     /locus_tag="PLT63_01880"
                     /inference="COORDINATES: protein motif:HMM:NF024825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOD97500.1"
                     /translation="MSHRVSRLNRWIVCLAVLALLLGFGPQAGSVLAAEKVIKIGTIF
                     PLTGPVATAGQRCQAAVQTAVEIINNKHPEIKVPLAKQQGILKGYKIVLVHADSQGKP
                     DVGKAEAERLLNQEGVWALIGSYNSSVSKPASFVAERMKKIFMCGSSSSAALTQRDMK
                     YFFRLAPTDATESVDFVEVLKWANKKNKANIRTLGVIYENSEFGKHAAEEAKKAAAAG
                     GFKVITDVPFTPGATNLNSEVQTLKKANPDAVFGAVLGADYSLWVRTMKQANFLPRIV
                     INYCSGYQDPVITKQLGDDANYFLGSSGYSPQFASLMPEVAAVEKIFKTKTNGVPFDG
                     NSIQEAVAMLVLAQAIEKAGGLDTEKVVKILYANTWDSPLSLGGKVKFVKGGQNVMAK
                     SIVTQLQGGQYKRIYPEKMADAKIVFPMKPWDKR"
     gene            7285..>7814
                     /locus_tag="PLT63_01885"
     CDS             7285..>7814
                     /locus_tag="PLT63_01885"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOD97501.1"
                     /translation="METLIQVLIDGLLSGMVYALVAAGLCLIWGVMDVINFAHGEYLM
                     VGMYVSYWLGFLAQVDPVVSVFAAGAFVFLLGALTYKLIIRYTIGRPGLVALLATFGL
                     AMFLKNLCMNRFSPNFLILSDTWLGGKTFMIGNLIFPVPQLAAGVLSLVVIGLVYALI
                     NATRFGWAVQATAMDR"
     assembly_gap    7815..7914
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7915..8300
                     /locus_tag="PLT63_01890"
     CDS             <7915..8300
                     /locus_tag="PLT63_01890"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOD97502.1"
                     /translation="INATRFGWAVQATAMDREAAELMGINTERIYILIFGIGGACVGF
                     AGGIMPSYLAVHPEVGSMFGLIAFVCVAMGGFGSIPGAFLAGLLVGVVEALAGFYIAP
                     VFKYIAVFGLYLAVVFLRPKGIFGW"
     gene            8321..9328
                     /locus_tag="PLT63_01895"
     CDS             8321..9328
                     /locus_tag="PLT63_01895"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOD97503.1"
                     /translation="MLKKDGAWLVFTVLLVLLPFVPFLGENSFRMHIMILILLFAAMS
                     QAWNIIGGYCGQVSFGHSVFFGIGAYGAGMAVVTYGVPPWPGILIGMVVAAVVAVVIS
                     WPCFKLSGHYFAIATFAIVEIFNRGFLVWDWVGGALGLDYPLVEEGLWNLMWHDSKTG
                     YYYCALALFLITFGLVRWLEGHRFGYYLRAVREGQETAESLGVNSTVVKLTAMALSAA
                     LAALCGSFFVQYNLRVDPPMVMSLDMSMKFVLITVLGGSGTLVGPLLGAAVLIPLQEY
                     SRALWGGLGGGVDLIIFGLLIIIIVVKQPAGIIGIIRDIRKYAARRRMAGKEEANHGA
                     P"
     gene            9315..10073
                     /locus_tag="PLT63_01900"
     CDS             9315..10073
                     /locus_tag="PLT63_01900"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD97504.1"
                     /translation="MALLEVREVTKKFGELVANDRVSFTVETGTIVGLIGPNGAGKTT
                     LFNCITGLYPADGGAIHFDGREITALKPYEIARRGAVRTFQVVRPLKEMTVFDNVLVG
                     AFLKTGDRRTAAARAEECLRICNLEPFRDRPAGGLPIGGKKRLEMARALATQPKLLML
                     DEVMAGMTSTEIKTSVEVILRLRESGITLMIVEHVMEGIMPIADKVVVLDGGIKIAED
                     APGNIVNDGRVIEAYLGAKYSQRFKARKEGGDLG"
     gene            10066..10782
                     /locus_tag="PLT63_01905"
     CDS             10066..10782
                     /locus_tag="PLT63_01905"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD97505.1"
                     /translation="MAEPILKVEDLHAGYDGLPVVFGVSLEVMPGELVAIVGANGAGK
                     TTTMRTICGLAHPLKGRIVFNGTDISGLPAHRILKLGISYVPEGRRLFAKLSVEKNLA
                     LGAYAENSPAEVKRRQEEIMEIFPILRERARQTAETLSGGEQQMLAIARGLMSRPRLL
                     MLDEMSLGLMPSLAEKMMETVVAVNRAGTTVLLVEQMVQETLEIAHRGYVIQNGRIVQ
                     SGAARDLLDSETVRKAYLGM"
     gene            complement(10816..11145)
                     /locus_tag="PLT63_01910"
     CDS             complement(10816..11145)
                     /locus_tag="PLT63_01910"
                     /inference="COORDINATES: protein motif:HMM:NF017273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HOD97506.1"
                     /translation="MMDEGLILLTFAGMGAVTYIPRWLPLHCLAEKKLPAWFESWLDF
                     IPASILSALLLPSLITGGHPRSLDLTRPELIVAVPTFLFAVKTKSLGGTVLVGMALFW
                     LAGRFFG"
     gene            complement(11142..11879)
                     /locus_tag="PLT63_01915"
     CDS             complement(11142..11879)
                     /locus_tag="PLT63_01915"
                     /inference="COORDINATES: protein motif:HMM:NF015549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HOD97507.1"
                     /translation="MNAPPGPSSTEGRPDAIRPGLAAAWPICLGYIPIGAALGVLAQK
                     AGLHPLDVGVMSLLVFAGSAQFIGISMLSAGAGTLAVIMTTFVVNLRHLLMSAALAVH
                     LRGLPKGVLALYAYGVTDESFAVNMTRFRTGSWDWRRALVVNTVSNLTWIAATIIGCY
                     AGELIPAGAFGIGYALTAMFICLLIMQTRTRLHILAAAVAGLLAVGLSLLMEGNLYVV
                     IASLVAATVCLPLAGGKRISTAERNGP"
     gene            complement(11876..12064)
                     /locus_tag="PLT63_01920"
     CDS             complement(11876..12064)
                     /locus_tag="PLT63_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97508.1"
                     /translation="MKRFLLFMLVILMGAMFLSVAGCAGGRDAARDKASSEETYRNEA
                     NFERAQDRQRLDHGGSGF"
     gene            complement(12112..12480)
                     /locus_tag="PLT63_01925"
     CDS             complement(12112..12480)
                     /locus_tag="PLT63_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97509.1"
                     /translation="MRFAAAKIAVLLISSAVLLFLGFNPPWKCSLHIGNTIISKHWGL
                     HTIMQPPNVFDYFYNLGDKFNLLLVDAGRYDPRILNAFEFQIDYPLTAIFMALTAVIT
                     LWMLWILKLAGELAAREKGL"
     gene            complement(12490..13464)
                     /locus_tag="PLT63_01930"
     CDS             complement(12490..13464)
                     /locus_tag="PLT63_01930"
                     /inference="COORDINATES: protein motif:HMM:NF020279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly factor Coa1 family
                     protein"
                     /protein_id="HOD97510.1"
                     /translation="MTTLPNMKLPDLLTQYMRKDRAPGPGSEKAYEPIQPGSGKKDYQ
                     PIRPKGGSARTWPFMLVFIALAAVFALHEFVRDSEPFKIASSFVKENPSIREDLGEIK
                     EIYPWFPVSIKAAGNTAHVKLTLRIKGQNGTGKAYVTLVYVNNWQIVAAMYENRQGRL
                     RNLTPGKERGETPRPAPAARGDSLDAGHRFFRQNDFPRALAAYTQALAADPNNDAAYY
                     WRGRTYYKMNLPDKAAADFTHTTALNPNHVEAHNWLGWLSEQEKRYDECVVHLTRAVA
                     LKTDNAWGYYHRGRCYHQQGKKNEARQDAERSCRLGYDQACKILKQLP"
     gene            complement(13529..15181)
                     /locus_tag="PLT63_01935"
     CDS             complement(13529..15181)
                     /locus_tag="PLT63_01935"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOD97511.1"
                     /translation="MTIRTKLILVYGALAIFFSTLFSFIGVVRITENARDNAVKMESA
                     IRGGLEKFLSEKAYALAGQIEIYLRYHPFDIHDPELRKIAVQKIQRTGYSGLHDGVGK
                     QDGKYLFHPNPAIEGQYLSAFRYKLPALWEAIRENLNGRPVSRYYLWEEKDGTFREKF
                     FVGIPVRGTDCILFATVYVDEFYQPLYQAQETITREKEASIRIFLISSFLATVVLILV
                     SVFIARSFSRPIHKVALHARRIGAGDLESRLHIPTGDEMEELAEVLNKMSADLSAYIK
                     NLTETTAVKERMEGELQLAREIQHSIIPHTFPPFPDVREFDIYGTMIPAKEVAGDFFD
                     FFFIDPDKLGIVIGDVSGKGVPAALFMSMARALVRVYGLAGEGPARTLGKTNRMLAAD
                     NDASMFVSVIYAEYDLWTGRMVLANGGHNPPLLCSAGASRRLDTQPDLFLGPFPDAAF
                     QEWSFTMAPGDAVIFYTDGVTEAQTSSGSLYGDERLQCRYDGRQDRDPQALCRDLLAD
                     VREFCRDAPQYDDITILAFRVNEFRARSGSELMDSQTPGNGV"
     gene            complement(15480..16781)
                     /locus_tag="PLT63_01940"
     CDS             complement(15480..16781)
                     /locus_tag="PLT63_01940"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HOD97512.1"
                     /translation="MTPISAGIVGFALLIALMFLRIPVAFVMAMVGFVGFGWLVSWPA
                     ALSLLARDFFSVFSSYNLTVIPLFILMGQVAHYSGISGRLFNAAHKFIGHLPGGLAMA
                     TIGACAFFAAICGSTTATSATMATVALPQMKKFNYDPALATGVVAAGGTLGILIPPST
                     IFIIYGIMTEQSVGKLFVAGVLPGILLAALFALVILVWSRLQPGLCALAPKASWREKF
                     ASLAGVIETIALFFIVMGGLFVGLFTPTEAAGIGALGTYVIALIGRNLTWRSFVRSLS
                     ETVRISCMIMTIVAGATVFGHFLAVTTIPTEVGAWVAGLPVPPVVVMALIIVTYLFLG
                     CLMDSLAMIMLTIPVFYPVVTTLGYDPIWFGVIIVMVGGMGVITPPVGINVYVVAGIA
                     RDVPLHIIFKGSAYLMCAMIILTALLIIFPQIALWLPGLMG"
     gene            complement(16778..17260)
                     /locus_tag="PLT63_01945"
     CDS             complement(16778..17260)
                     /locus_tag="PLT63_01945"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOD97513.1"
                     /translation="MHRIERFVNNLSRPAGLAAGTALISMMLLTCADVVMRFFDLPIP
                     GTYEIIGFLGALAVALALAATSMEKGHIAVDLLVNRLPQRWQILLDAASSLFGLTLFA
                     ALTWQSVLYALDLRQSGEVSATLGMPVYPFAAGIAAGCALLCLVLGAEFGRGLRRIGS
                     "
     gene            complement(17267..18286)
                     /locus_tag="PLT63_01950"
     CDS             complement(17267..18286)
                     /locus_tag="PLT63_01950"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HOD97514.1"
                     /translation="MKKQFITALSIISLVWTFSAGAVEAATSLTYSIFFPPSHGQAKA
                     GEAWAKEIEKRTQGQVKITILAGGSLTPADQVYDGVLKGISDLGMSCFAYTRGRFPVM
                     EVLDLPIGYPNGRVATAVANEYFRIFNPQELKGVKVLYLHAHGPGLLHTNRPVATLDQ
                     LKGMKIRSTGLSAKVVSSLGAVPVAMPQGSTYESLQKGVVEGTFGPIEVLKGWKQAEV
                     IKSTTDCPEIGYTTAMFVVMNLKKWNALPKHIQKVFEEVSSQWIDVHGKAWDDLDQEG
                     RRYTLSLGNKIIPLSKQEGARWKKAVRPMLNEYIKTTTAKGLPGQKAVAETERLIKKY
                     AKTYK"
     gene            complement(18626..18937)
                     /locus_tag="PLT63_01955"
     CDS             complement(18626..18937)
                     /locus_tag="PLT63_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97515.1"
                     /translation="MPLLVGGAVAAILGLIGLVVWWNSFAVIVKGALPILMILGGGLA
                     MYVGYDDIQERIREEKKRQDDKLEKAQEEIEQIRAKAELYREELERLRVETLKSKENN
                     P"
     gene            19131..19505
                     /gene="hisI"
                     /locus_tag="PLT63_01960"
     CDS             19131..19505
                     /gene="hisI"
                     /locus_tag="PLT63_01960"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HOD97516.1"
                     /translation="MIVPDFNRAGGLVPAIVQDDETGEVLMLAYMNRQSWEKTLVTGL
                     ATFWSRSRQALWVKGETSGNVQEIKEIRIDCDLDAVLLKVRQRGGAACHTGYRSCFYR
                     RIAAGGLEVCGEKIFDPEEVYK"
     gene            19502..20374
                     /gene="hisG"
                     /locus_tag="PLT63_01965"
     CDS             19502..20374
                     /gene="hisG"
                     /locus_tag="PLT63_01965"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOD97517.1"
                     /translation="MNTLRLGIPKGSLEASTVDLFKRAGWRITYDSRNYFPDIDDEEI
                     TCILVRAQEMSRYVEEGILDLGLTGRDWIVENDSDVVTVTDLIYSKVSARQSRWVLVV
                     RQDSEIQSIADMRGKRISTELVNFTKNYFRERGIDVHVEFSWGATEAKVVEGLVDAIV
                     EVTETGSTIRANKLKIIHELMKTNTQLIANRLAWEDPWKRQKIDQIKTLLTGSLQAMG
                     KVGLKLNVAKENLGRVILVLPSLKAPTISSLYSEEWYAVETIVDSAIVRDLIPILQEA
                     GAEGIIEYALNKVI"
     gene            20404..20988
                     /gene="hisB"
                     /locus_tag="PLT63_01970"
     CDS             20404..20988
                     /gene="hisB"
                     /locus_tag="PLT63_01970"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HOD97518.1"
                     /translation="MRKGKIKRKTRETNISVEMELDGVGRGEIDTKIPFMDHMLNLLA
                     RHGLFDLKISGQGDREVDDHHLVEDLGICLGNAVKKALGDKEGIARYGAALAPMDESL
                     ASVALDISGRPCLVYHAELGEARIGAFDPSLLREFFKAFSDHGGITLHINVLYGKNNH
                     HMAEAAFKAFALALRSAVQLDERIQGVMSTKGLL"
     gene            21030..21848
                     /locus_tag="PLT63_01975"
     CDS             21030..21848
                     /locus_tag="PLT63_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97519.1"
                     /translation="MNNYISANKISYLRKSSPPSPANHAAAPGRNHRRRGFKAALSLL
                     LWVLLLATTGCQSASGVSTSLLRDRDIVFKRLAVMPFQQVSPEIVATYSARSSLPASV
                     LKTDNDPRSPERYLQDLFMELMAKHPQFDLVSPDRVGGTYEGVSTGSLKATLPKALKE
                     AGTELGADGLVVGYLYRWRERRGYDYSVEKPASVFFEIQLYRTVDGMMVWKGVFDKTQ
                     KSLMENVLGASYFVKDRGRWLTAKELAAEGMADIVGRWPGVAKKEAKKAESRPE"
     gene            22002..22739
                     /gene="hisA"
                     /locus_tag="PLT63_01980"
     CDS             22002..22739
                     /gene="hisA"
                     /locus_tag="PLT63_01980"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HOD97520.1"
                     /translation="MIVIPAIDLKDGRCVRLAQGDFQRVTVYAEHPASVAAQWQEQGA
                     ERLHVVDLDGSLAGSPRNREAIRAIIGAIEIPVELGGGIRDLETVADYLDLGVRWVIL
                     GTAALRNPGLVREACRTFPDRVILGIDAREGLVSIQGWTETTAHSAVELIRSYVDCRL
                     SAVVYTDIKRDGMETGVNVDATRALAEATGVPVIASGGVSGMADIEKLMEVAPSGIIG
                     VIAGRALYTGALSLAAAVRQTKAGRNG"
     gene            22822..23166
                     /locus_tag="PLT63_01985"
     CDS             22822..23166
                     /locus_tag="PLT63_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HOD97521.1"
                     /translation="MDDCIFCKIIKGEIPCRKVYEDESVLAFDDIHPMAPVHVVIIPK
                     RHLATLLDVGEDTLPVVDAMIRVAQEVARRKGIDKRGFRTVINCNEEGGQVIFHLHMH
                     LLGGRKLADGLG"
     gene            23251..23697
                     /locus_tag="PLT63_01990"
     CDS             23251..23697
                     /locus_tag="PLT63_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HOD97522.1"
                     /translation="MELKNKLEEELVKAAKAKEKLRLSALRLVKNAIHNKEIDIKRET
                     TEAEFLQTLASMVKQRKDSIEQFEKGGRTDLVEKETAELKVIQEFMPRQFSEEDLDAA
                     IEKAIRETGAAGPKDMGKVMKALMPVISGRADGKVVSEKVKGRLAG"
     gene            23707..25494
                     /gene="dnaG"
                     /locus_tag="PLT63_01995"
     CDS             23707..25494
                     /gene="dnaG"
                     /locus_tag="PLT63_01995"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOD97523.1"
                     /translation="MMQGSIPEDKIDEIKRRINIVDLVAGYVNLKQAGRNFIGLCPFH
                     QEKTPSFTVSPEKQIFYCFGCGEGGNAVTFIMKQNGLSFPEAIRYLARKTGVALPTRP
                     LSNKEKGELSLREKMLRLHGLAAAFFSERLEGQEGLAARQYLRERGISAAVVKEFRLG
                     YSPPGWRHLKDYLERKRMPLDLAEQAGLLVKNERGDFYDRFRGRLVFPIEDQSGQIIA
                     FGGRIMGEGMPKYLNSPESALFSKGKNLYGLVKAREEIRKSGYVILVEGYFDLLALWN
                     ADIRNVVATLGTALTRDHVDILRRYAPEAAVVFDGDEAGKKALARSLALFVEGNLPMR
                     AVMLPPAADPDDYVRGSGREAFLALVATAPPAVEYYVDNFIGTPESMEGGREALRTAM
                     AFIGKMESDAERNLFIKRVAQRLGVDEDVLKAEVNRGRRKKTAEPEAGEKTLPSTAGG
                     DALEASFIALLLDHPRMMPAVITGEALTYLQNEELKRLGTAAVAQYERQGGIDLEDFV
                     ARLSPGALREAILGRLVDSVSWEGETADRVCRDMIRQIKRRWFKEKRRSLNVSLVRAQ
                     QAMDMESCRRLLAEKTELIEKEKSMLSNG"
     gene            25509..25868
                     /locus_tag="PLT63_02000"
                     /pseudo
     CDS             25509..25868
                     /locus_tag="PLT63_02000"
                     /inference="COORDINATES: protein motif:HMM:NF015912.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
     gene            26514..27260
                     /gene="rpoD"
                     /locus_tag="PLT63_02005"
                     /pseudo
     CDS             26514..27260
                     /gene="rpoD"
                     /locus_tag="PLT63_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
     gene            27355..27430
                     /locus_tag="PLT63_02010"
     tRNA            27355..27430
                     /locus_tag="PLT63_02010"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27388..27390,aa:Ile,seq:cat)
     gene            27576..28301
                     /locus_tag="PLT63_02015"
     CDS             27576..28301
                     /locus_tag="PLT63_02015"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HOD97524.1"
                     /translation="MNKNLMHLIALQKIDTETQKVLQSRKDLPLRLARLDTDFAAVRE
                     ALEEERNRLEAKNQTHRELEEALKKGMEQLRKTRDRIHEVKSNKEYQAVLKEIETLEQ
                     KHGIVEDKIITILEEIDTMKTSLAIREGEFQASGRRYEEEREKLEKRIGSIESQLQER
                     QRQGESMREKIPKNVLKRYDMTKNVNQGLAVVSAWKETCDGCHMSIPPQLYNELLEDT
                     EELIMCPNCRRIIYWYDQNKESV"
     gene            28299..28689
                     /gene="rnpB"
                     /locus_tag="PLT63_02020"
     ncRNA           28299..28689
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLT63_02020"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(28827..31235)
                     /gene="ppsA"
                     /locus_tag="PLT63_02025"
     CDS             complement(28827..31235)
                     /gene="ppsA"
                     /locus_tag="PLT63_02025"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HOD97525.1"
                     /translation="MSDDRLTIWFDELQLADIPQVGGKNASLGEMRRELIPKGVSIPD
                     GYAVTAYAYRYLLESAGIIDQIKTVLSDLDTHNIENLKTRGHKVRELIYNAPFPDDLS
                     AAIITAYRKLCEEYGENTDVAVRSSATAEDLPDASFAGQQETYLNITGDEALIEACKR
                     CFASLFTDRAISYRVDKGFDHLSIALSIGVQKMVRSDLAASGVIFSIDTESGFKEAVL
                     ITGAYGLGETVVQGTVNPDEFYVFKPTLKQGYRPIVQKKLGTKEIKMIYAERRGGEAT
                     ETVSVPVEQRRKFCLNDDEILQLARWTTIIEDHYSQQAGYYKPMDIEWGKDGRTGELF
                     ILQARPETVQSQKDASVLENYILLEHKNPLVTGTAVGSKIGAGPANIIESVEQIMNFK
                     KGEVLVTDMTDPDWEPVMKIAAAIVTNKGGRTCHAAIVSRELGVPCIVGTENGTTALK
                     GVKEATVSCAEGEIGNVYEGLLKFDVQRVDAKNLERPKTMIMMNVGNPENAFNLAAIP
                     NDGVGLARLEFIINQFISIHPMALIQFDKVTDPAIREKIAALTHGYDNRADFFVDKLA
                     QGVAKIAAAFYPNDVIVRMSDFKSNEYKNLIGGQYFEMDEENPMIGFRGASRYYDERY
                     RDAFALECEAMKKVRNEMGLVNAKLMIPFCRTVEEGKKVVALMAEKGLVRGKNNLEIY
                     MMVEIPSNVILIEEFGKIFDGFSIGSNDLTQLTLGLDRDSFLISHIFDERNQAVMDLI
                     RIAITKANKMGKKIGICGQAPSDYPEFARFLVECGISSISLNSDTVIKTTIDIKETER
                     TLRK"
     gene            complement(31393..32706)
                     /locus_tag="PLT63_02030"
     CDS             complement(31393..32706)
                     /locus_tag="PLT63_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97526.1"
                     /translation="MSEKKQDKQFFTVARVFFLLIVIPLSLMAILIANGIFKLGVTVK
                     ERAVTVLDQKSQEEIKIRAMNIADEVASFLTERKKDVLIATIIPATESAYKQFVSENR
                     RNVWIKDGDKLKQISVPIYTEMTLIDKSGNEVIKITNGEIAPKSRLTNVGIPANTTYK
                     SETYFASAKALNKGDVFVSPVTGWYANRADFEKGKRFSGVIRFATPLYGREGLQGVVQ
                     LALDYRHLAAYTDHVIPTQATPVFEADAATGNYAYMVDNRGLMISHPNDYHVEGLYSD
                     GKPVPALTEQSSAELTKKGEEVLNLNLMGFVDPNLPLIAKDAASGKPGIKTYKFAGHT
                     KFVAYAPIKFFAPDYPAPGGFGWIGMGVDVEKFNESAAQATQNIQKEAKAWSTTIILI
                     LVISIILLFAIAGLLSRGISRSIAAEVPADSQGEGPFYDDEEEDK"
     gene            complement(32758..33156)
                     /gene="atpE"
                     /locus_tag="PLT63_02035"
     CDS             complement(32758..33156)
                     /gene="atpE"
                     /locus_tag="PLT63_02035"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HOD97527.1"
                     /translation="MKGEEKMLGKFGKKLLTLMTTMAAVLLSSPVVFAAGEALPGAGE
                     SYVKVIYAVGAMIGAGFAIGLGAIGAGTGIGNAASGACQAVGRNPGVQGKIMTVMLVG
                     MAMAESVCIYALVVALVLLYANPYVKYFLG"
     gene            33533..33715
                     /locus_tag="PLT63_02040"
     CDS             33533..33715
                     /locus_tag="PLT63_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97528.1"
                     /translation="MQEILTAVTYLVVWLICQRVIGIESGLVNLIISLSAAVGVYVLM
                     AVKEHNEKKKREDRHE"
     gene            33708..35741
                     /locus_tag="PLT63_02045"
     CDS             33708..35741
                     /locus_tag="PLT63_02045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOD97529.1"
                     /translation="MNKAVEEALVQERKVIVGIIEGSPIPTFVINRKHEVIFWNRACE
                     ELTGFAAGDMIGTDRHYMPFYGTKRPLIADLIVDQDIVSLEKLYGSKRVRKSVKVEGA
                     YEARDFYIDLGGQSRHLYFLAAPIYDEQGEIIAAIETLQDVTRERVMELSLKEHAENI
                     REELEKNITLRKTIEGIIEGSPIPMFVIGKDHKIMFWNRAFTELSGYEGKYFIGTDRQ
                     YVPFYPEKRPVIADLIVNDDIESLEKFYGKKNVQKSPIIEGAYEAWDFYPNIGGRNRH
                     LYFLAAPIRDDRGEIIAAVETLQDITREEEMSLYLKEYAETLENELKENIRLRQEIEG
                     LYNYMQAVLESSPDTLFELNSDGIITFASRDRRHDGMQADQIKGKHFTEYAPAHKEYL
                     LEKWQEIKKGVFKPFEMDVTTRDGSRQYLLITPCPLKGSADRYVMVRREITEFKELEK
                     KYYESQKLAAIGQLSAGIAHEVRNPLSSIKMSLQILEKRLQPTGNDLKRFRIAQREVE
                     HLEELVNDVLIFAKPGEPKRMPTDLRRAIENALEMVEKAVTDKHIAVVITMAEELSHP
                     NIDGAMMEQVFQNIFRNAVDAMEEGGQLRISARDNGGATSAVVIEIADNGCGMDEEVL
                     SHLFNPFFTQKRYGTGLGMTQVKKIIDLHKGNIDVESRKGEGTNIVITLPMNI"
     gene            35767..37146
                     /locus_tag="PLT63_02050"
     CDS             35767..37146
                     /locus_tag="PLT63_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOD97530.1"
                     /translation="MAKILVIDDDSSIAESLDLYLTEEGYTVYTAATGTDGLNAFVKH
                     APDLVVLDIRLPDIDGFTVLEDLREEDENVKVIMITAYHDMDSTIKAMKGGAFDYIHK
                     PINVEELDMAIRKALKTLEMEKKISGLLNEPSRSFKVGDIIGNTKEMKEIFKTIGVVS
                     QSRTTVLVEGESGTGKELIAKVIHNNTSPDEPYIAVNCSAIVETLLESELFGHEKGSF
                     TGAITRKLGKFELARYGTVFLDEISEMSVNLQAKLLRVLQEMDFERVGGKDSIKVNAR
                     IVTATNKDIKSMVKEGKFRDDLYYRLNIVAIRLPPLRERREDIPALVDYLLTKINREL
                     HKRVIGISDEMMDLFMTYRWPGNVRELENLLTRAVVVTKSQVLTRNDFPDLSEEVVAA
                     DDGKGKDAVEINAGGLLTLEAVEEQYIRKVIKENSHRTKGELCEILGISRPTFERKLE
                     KYGLAIERD"
     gene            37156..37746
                     /gene="yihA"
                     /locus_tag="PLT63_02055"
     CDS             37156..37746
                     /gene="yihA"
                     /locus_tag="PLT63_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HOD97531.1"
                     /translation="MKIRSAEFVKTALLPSHYPPPQFPEIAFAGRSNVGKSTLINTLA
                     NRVGLARASRTPGRTQAINFFLVNERFCFVDLPGYGFARVPDQVRRDWGPMVEAYLAG
                     RENLTIIILILDIRRDLREDDRNFLLWMEEKGRPWQLVLTKTDKLSHQQALTRRRHIE
                     AQAGIPVPREPILFSARTGQGRGPLWRIIEAAAETP"
     gene            37997..>40544
                     /locus_tag="PLT63_02060"
     CDS             37997..>40544
                     /locus_tag="PLT63_02060"
                     /inference="COORDINATES: protein motif:HMM:NF009410.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HOD97532.1"
                     /translation="MINDNEKIGSVMVVGSGIAGIQASLDLANSGLKVYLVEKDISIG
                     GVMAQLDKTFPTNDCSACILSPKLVEVGRHPNVEILTRRTVNAVEGEAGKFTVRMTKA
                     PRFIDLDKCTGCGDCAKVCPINVPSDFNEGLSERQATYRKFPQAIPSGFAIDKLGTSP
                     CKAACPTHISVQGYVALIAAGKYKEALALIKKDNPFPIVCGRVCNHPCESACMRGKVE
                     EPIDIMHLKRFVADLDLKGETRFIPEKKESKGKKVAVVGAGPAGLTCAYYLAAEGYDV
                     EVFESLPVAGGWLAVGIPEYRLPKDVLRAEIKVIEDLGVKIHLNTAVGKDIPFGKLQS
                     DYDAVFIGCGTVNSSKLNIPGEDMQGVIHGVDYLKRVNLGEKVFLGDKVAVVGGGNVA
                     MDAVRTAVRTGSKDVFILYRRSRAEMPASPEEIEEALEEGIKMEFLVAPKRVVGESGK
                     VTGVECTRMELGEPDASGRRRPVEIKGSEFIVPCDALIPAIGQEADLDFVPQESGIAI
                     NKWKNFDVDPVTFAANVPGVFAGGDVVTGPQTVVKAVYAGKEAAVSIDRYLKGEDVAA
                     GRAKDWKQGLADKADVSKVAKIARMKYPHMKPEDRRTNFSEVGIGFTEEEAVAEAKRC
                     LACGICSECYQCVDACIAGAINHDDTFEEETIEVGAIIAAPGFEVFDAKHRGEYGYGI
                     YPNVVTSIQFERILSASGPYFGHVQRISDGREPQKIAFIQCVGSRDTSCGNSWCSSVC
                     CMYATKEAIIGKEHAKGLEPTIFFMDIRAHGKDFDRFVNRAKDEYGIRYIRSMPSTIK
                     ELQQSNNLLLKYVQEDGSLVEEEFDMVVLSVGLCPPSEAAELAKALGIELE"
BASE COUNT         8690 a        11305 c        12317 g         8032 t
ORIGIN      
        1 tccccgtgct tttccatact gaaaaaaact cggcgtggaa aggcaaaatg ttcgcttttt
       61 ccgccagatt ttctttgaca cggcaaaaac ctttacctta tacttccccc gcctccggag
      121 acggcgagat cccgatgtgt cgcgattgcg cacgtcaagc tccgctaaaa aaacattaat
      181 cttcattaaa aagcatcatg cttcgcttgc cgctctttga aactgaatat tgttcataag
      241 atactgaatt ccagaacttt ttacgatgcc gtcacaaagg ggacattttt ttacttacta
      301 tgagaatacc attttcatac ttcgtggtgc tccatggggc gtgagggttt tttgcgatgt
      361 catcaatctt ggagggagga ttatgattaa aagcaccagc gcgagttaca gcatcttgat
      421 gcgcgtggag ttgaacaacc ggccgggcat gctggggaag gtggtctccg ccattggcaa
      481 ggccgggggc aacatggggg cggtggatat cgtcggcttc aaaggcgact gcatcatccg
      541 agacgtcgtg gtccacgccg ccgatcacga catgatggaa aagatcctcg ccgccgtccg
      601 gcggattgcc ggcgccgccg ttatcgactt cgccgaccgc accttcatgg cccacatggg
      661 gggcaagatc gaggtcaaaa gcaagatccc cctgagtagc cgtgatgacc tggccatggc
      721 ctacaccccc ggggtcgccc gggtctgcga ggccatccat gcggatccgg agcgggccca
      781 cgacctgacg atcaaaaaga actgcatcgc catcgtcagc gacggcaccg ccgtcctggg
      841 gctgggggac atcggtccga aggccgccat gccggtgatg gagggcaagg ccctgctctt
      901 caagaacttc ggcggggtgg acgccttccc catctgcctg gatacgaaaa tccccgatga
      961 gatcgtggag acggtgcgcc gcatcgcccc cggttttggc ggcatcaatc tggaggacat
     1021 ctccgccccc cggtgtttcg agatcgaggc gcgcttgaag gagattctgg agatccccgt
     1081 cttccatgac gaccagcacg gcacagcggt ggtgatcctg gcggggctcc tcaacgccct
     1141 gaagatcacc ggcaaggaca tcggcgagat cctcgcggtg gtgaacggcc tgggcgccgc
     1201 gggagtggcc gccatcaatc tcctccgggc cgccggggtc cggcgcttca tcggctgcga
     1261 ccgcaagggc atcctgtacc ggggccggaa ggaaaacatg aacccgatca aggaaaagct
     1321 cgccctggag accaacgagg agcaaatcaa aggcgacgtc tttcgggccc tcaagggagc
     1381 ggacctgttt ctcggcgttt cggaagggaa tctcctccat gccgcccacg tcaagaagat
     1441 ggcaaaggac cccatcgtct tcgccatggc caacccgacg ccggagatca tgccggaaga
     1501 ggccgcgaaa tacgtgcgga tcatggccac nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn cgacgccgga gatcatgccg gaagaggccg cgaaatacgt gcggatcatg
     1681 gccacgggcc gctcggatta tcccaatcag atcaacaatg tcctctgctt ccccggactc
     1741 ttccggggag ttctggactg ccacgcccgg gagatcagcc ggcagatgct cctggccgcc
     1801 gcccgggcca tcgccgccag cgtcgagacg gagcagttga tggaggacta catcatcccc
     1861 agcgtcttca acctcaaagt tcaagaaaac gtcgccgccg cggtgcggga ggtcgccgtg
     1921 accactggcc tggcccggga cgtccacgaa ttttccctgt tctgagccga cgatgaaaaa
     1981 gatatcgccc tcgcagaaga tcttcatcgg catggccctc ggtttggcgg ttggcttgct
     2041 ctttcctcat ttcgcccctt atctcgaacc gctctccacc atcttcatcc gccttgtcaa
     2101 gaccatcgtc gtacccatca tcttcgtatc ccttatcgtg gggatcgccg gacacggcga
     2161 catgaaggcc gtgggccgga tggggctcaa agccattgtc tatttcgaga tcgttaccac
     2221 cttcgcttta tgtatcggtc tgttggccgt gaacctgttg cgccccggcg atggcgtgga
     2281 catcggcggc ggccgggagg cggtggtggc cggcaaggtc aacagttttt cggacctcct
     2341 ggtacacatg tttccggaaa acgtgctcga atcggcggcc cggggcgatc tcctccaggt
     2401 agtggtgttc acggtgatct tttccgcggc cctgatcatg atcggagaga agaaaaagcc
     2461 catcatcgag ctgtgcgagt ccgtcacaga ggtaatgttc aaatatacga acatcgtcat
     2521 gcgcgtcgca cccatcgggg taggggcggc gctggccgtt gttgtgggaa gcaagggaat
     2581 atccgtagta gtgcacctgc tgggactgat tggctgcctc ttcctctccc tggtggtttt
     2641 ccttatcgtg atcatcgtgc ccgtcatgct tatcctccgg atccctctcc ggaagttctt
     2701 tgccgccgtc aaggatcccg ccgtaatcgc cttctccacc accagttccg aggctgcttt
     2761 gcccaaggcc atggaggcca tggaggcctt cggtgtcccc cggcgcattg tatccttcgt
     2821 tattccggtg ggatacagct tcaatcttga cggcacgacc ctctatctgc ccatggcggc
     2881 cgtatttgtg gctcaggcct cggggatcga gatgagtatc ggtcagcagt tcttcatggg
     2941 actcaccatc atgctggcca gcaagggggt ggcgggaatc tcccgggggt ccatggtcat
     3001 ccttgccggg ctcctcccca ccttcggcct gccggtatcc gccgtggcgg tgctcctttc
     3061 catcgacgcc atcgccgata tggggagaac gacggtgaat gtcatcggca actgtcttgc
     3121 ctccgcggtc gtgaccaagt gggagggcct gccgctcaag tcgggggaag aggcgtcgcc
     3181 aaccgcgccg ccaggcgaca gttgaccgcc ggcgtgcagc atggttaagt cagaaaacga
     3241 aaaacggcgt ttcgtagatc accacaaacg ccggcgtatc gtaacaagtg gttttcctcc
     3301 tcccccttca agggggaggc aaggaggggg atgggccaaa aacaggtgat ggtggggggg
     3361 ccttaaaccc atacccaccc cggccctccc cttgaagggg agggagctac agggaaccag
     3421 accaatatca ccaccccggc ccttgaaggg aaggaaaatt attaaggagg gtgccatgac
     3481 cgcgaaaaga tttcgcatcg aacacgatct actgggggaa aaggaggtgc cggccgactg
     3541 ctactacggc gtgcagaccc tgcgggcagt ggaaaacttt caaatcagcg gcatcaggct
     3601 ggggaggtac cggttcatgg tccgggccct ggccttcatc aagaaggccg ccgcccgggc
     3661 caacgccaaa ctcgggctcc tgccggaaga gatcgcccgg gccatctccc aggcctgcga
     3721 tgaggtactg gccgggaaat atgaagatca gttcgtcgtc gatatgatcc agggcggggc
     3781 gggaacctct tcgaacatga acgccaacga ggtgatcgcc aacgtggccc tggaaaagct
     3841 cggctatgaa aaggggtgct acgacatcat ccatccgaac aatcacgtca acatgtgcca
     3901 gtccaccaac gacgtctatc ccacggcgat ccgcctggcc ctgatcttca agatccggga
     3961 gcttctcgaa tcgatgcact atctctacga cgccatcgag gataaatcca tcgagtttcg
     4021 ggacgtcatc aagatgggcc gcacccaact gcaggatgcc gtgcccatga gcctgcggca
     4081 ggaattctcg gcctttgccg tcatggtggg ggaagatatc gagcgggtga aggaaacgga
     4141 gaagctcctg agggagataa atctgggggc gacggccatc ggcacgggac tgaacgccca
     4201 tccggagtac gctcccctcg ctgtcctcaa tctccgcaag tttacggggg tcgaggtgga
     4261 gacgtcggtc aatctcgtag aggccaccca ggacacgggg gtttatgtcc aactctccgg
     4321 ggtgctcaag cgggtggcgg tgaaactatc caagatatgc aacgatttgc gtctcctctc
     4381 ctcgggaccg cgctgcggct tcaacgagat ccgcctgccc gccatggccc ccggctccac
     4441 gatcatgccc ggcaaggtga atcccgtcat cccggaggtg gtgaatcagg tggcctttca
     4501 cgttatcggc aacgacgtga ccgtaacctt cgctgccgag gcggggcagc tccagttgaa
     4561 cgccatggag ccgatcatcg ccttcagcct cttcaactcc atcaccttct tgaacaatgc
     4621 ctgtctcacc ctggcggacc gctgcatcaa ggggatcacg gccaatcggg aacagtgccg
     4681 cctcaacatc gaaaggagca tcgggctggt caccgccctc aacccttata tcggctatga
     4741 gaaggccacg gaagttgccc atgaggcgct ggagacgggc cggtcggtct atgagatcgt
     4801 cctggaaaaa ggctacctgt cccagaaaga tctggacagc atcatgtcgc cggaaaacat
     4861 gatcaaaccc cactatttcc aggggggata aaggcaagcg ggccatggtc gaaaggcata
     4921 taatcatgcc ggcgctagcg gcgctgctcc tggtcctcac gctggcctcc ggctgcgccg
     4981 gaccggccag tgacgatcgc tccctggaga tcacccggtt gaaaggctat ttcgtcgtcg
     5041 gcctcgacga ccactttccg cccctgagtt tccgcctgcc gagcgtcgac cggaccgtcc
     5101 gttacgagct gaatttggag aaaaaagcgg tggaaaagat caccgtcggc gacgaactgg
     5161 tggggttcga tatcgacctg gcgaagaagg cggcggaaaa gttgggcctg cgggtgattt
     5221 tccagccggt tccctgggac ggtataatcg cgagcctcga aaaaggggag atcgacatga
     5281 tctggagcgg cctttcggtc accccggagc gccgggagcg gatcatcttc tcccggccct
     5341 atctcgacaa ccgccagatt gtcatggtca aaagcggcgc cgccatctcc ggcaaggccg
     5401 atctgcaagg gaagacggtc ggcctgcagc ttggttcctc cagcgacacg gccctccacg
     5461 ccgaacgccg gatcgccgcc cacctcaaag aactcaaacg ctaccccgac gatgccgccg
     5521 ccatgcggga actggaggcg ggacgcctcg acgccgtcgt catcgacgaa gtcatgggac
     5581 gctacctcat cacccaacaa cccggagtct ttgctgttct cgccgatgat ttcgggcggg
     5641 aggagtacgc cgtgggattc cggaaaaacg accgggccct gcgggacgcc gtggaccggg
     5701 ccctcgggga gatgatgcgg gacggcgccg ccgatgccgt ggcgaagaaa tggttcgggg
     5761 cggcgattgc cgagaaatag atcggcggat caacagacgc tgccggacgg cctgccgccg
     5821 ccttttccat tgacatccgg atgattttca gatagtccct tcccggaaaa gtaagacccg
     5881 cttcggtatc aaatcataac aaccactaaa gaaaaggagg taagtctatg tctcatcgag
     5941 tgtcccgctt gaaccgttgg attgtatgcc tggccgtcct ggccctgctg ctgggtttcg
     6001 gcccgcaggc aggatcggtt ctggccgcgg aaaaggtaat caagatcggc accatctttc
     6061 ctctcaccgg cccggtggcc accgccggcc agcgctgcca ggcggccgtc cagaccgcgg
     6121 tggaaatcat caacaacaag catccggaaa tcaaggtccc cctggccaaa cagcagggga
     6181 tactgaaggg ctataagatc gtcctggtcc atgcggacag tcagggcaaa ccggacgtgg
     6241 gcaaggcgga agccgagcgg ctcctgaacc aggagggcgt ctgggccctt atcggctcct
     6301 acaacagctc ggtgagcaag ccggccagct tcgtggcgga gcggatgaag aagatcttca
     6361 tgtgcggttc gtccagctcg gcggccctca cgcaacggga catgaaatac ttcttccgcc
     6421 tggcccccac ggatgccacc gaatcggtgg acttcgtcga ggtcttgaag tgggccaaca
     6481 agaagaacaa ggccaacatc aggaccctgg gcgtcatata cgagaacagc gaattcggca
     6541 agcacgccgc cgaggaagcc aagaaggcgg ccgccgccgg cggcttcaag gtcatcaccg
     6601 acgtgccctt cacccctggg gccaccaatc tcaacagcga ggtccagacc ctgaaaaagg
     6661 ccaatcccga cgcggtgttc ggagctgtcc tcggggcaga ctactccctg tgggtccgga
     6721 cgatgaagca ggccaacttc ctgccccgga ttgtcatcaa ctactgctcg ggctaccagg
     6781 atcccgttat caccaaacag ctcggtgacg acgcgaatta cttcctgggc tcctcgggct
     6841 actctcccca gttcgccagc ctgatgccgg aggtggcggc ggtggagaag atcttcaaga
     6901 ccaagacgaa cggcgttccc tttgacggga acagcatcca ggaagcggtg gccatgctgg
     6961 tcctggccca ggccatcgaa aaggccggcg gtctggatac cgaaaaggtc gtcaagatcc
     7021 tctacgcaaa cacctgggat tcgcccctct ccctgggcgg gaaggtgaaa ttcgtcaagg
     7081 gcggccagaa cgtcatggcg aagagcatcg tcacccagct tcagggcggg cagtacaagc
     7141 ggatctatcc ggagaaaatg gcggacgcca aaatcgtctt ccccatgaaa ccgtgggaca
     7201 agcggtagca aaccggcgtt ccggtaagga caagggcgtg cggacctccc ccgttccggg
     7261 ggaggtcgcc gaacgcggta gtttatggag acactgattc aggtcctgat cgacggcctc
     7321 ctgagcggca tggtgtacgc cctggtggcg gccggcctct gcctcatctg gggcgtcatg
     7381 gacgtcatca atttcgccca cggcgagtat ctcatggtgg gcatgtacgt cagctactgg
     7441 ctcgggtttc tcgcccaggt cgatcccgtg gtctcggtat tcgccgccgg ggccttcgtc
     7501 tttctcctcg gggcgctgac ctacaagctg atcatccgct acaccatcgg tcggcctggt
     7561 ctggtggccc tgctggccac attcggcctt gccatgttcc tcaagaacct ctgcatgaac
     7621 cggttttccc cgaattttct catcctttcc gatacctggc tcgggggcaa gaccttcatg
     7681 atcggcaacc tcatctttcc cgttccccaa ctagcggcgg gggtcctctc ccttgtcgtg
     7741 atcgggctgg tctatgccct catcaacgcc acccggttcg gctgggccgt ccaggccacc
     7801 gccatggacc gcgannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntcatca
     7921 acgccacccg gttcggctgg gccgtccagg ccaccgccat ggaccgcgag gcggcggagc
     7981 tcatgggcat taacacggag cggatctaca tcctgatctt cggcatcggc ggggcctgcg
     8041 tcggcttcgc cggcgggatc atgccctcct atctggccgt gcatccggag gtgggttcca
     8101 tgttcggact catcgccttc gtctgcgtgg ccatgggcgg cttcgggagc atccccgggg
     8161 cctttctcgc cggactgctg gtgggagtcg tggaggccct ggcgggtttc tacatcgccc
     8221 cggtattcaa atacatcgcc gtcttcggtc tctatcttgc cgtggtgttc ctgcggccga
     8281 aaggaatctt cggctggtga actccgggca gggaaatagc gtgctcaaaa aagacggggc
     8341 ctggctcgtc ttcaccgtcc ttctcgtctt gctgcccttc gtgccgtttt tgggggaaaa
     8401 ctccttccgc atgcacatca tgatcctcat cctccttttc gccgccatgt ctcaggcatg
     8461 gaacatcatc ggcggttatt gcgggcaggt gtccttcggc cattcggtgt tcttcggcat
     8521 cggggcctac ggcgctggga tggcggtggt cacctacggc gtccccccct ggccggggat
     8581 cctcatcggc atggtcgtcg cggcggtggt ggccgtggtc atcagttggc cctgcttcaa
     8641 actgagcggc cactatttcg ccatcgccac cttcgccatc gtggagatct tcaaccgcgg
     8701 atttctggtc tgggactggg tcggcggcgc cctgggcctg gattatcccc tagtcgaaga
     8761 aggcctgtgg aacctcatgt ggcatgattc caaaactggt tactactact gtgccctcgc
     8821 cctgttcctg atcaccttcg ggctggtgcg ctggctggaa ggccatcgct tcggctatta
     8881 cctgcgggcg gtgcgggaag gccaggaaac cgccgaatcc ctgggggtga acagcaccgt
     8941 cgtgaaactg accgccatgg ccctttcggc ggccttggcc gccctgtgcg gctccttctt
     9001 cgtgcagtac aatctccgcg tcgatccgcc catggtcatg tccctcgaca tgtccatgaa
     9061 attcgtcctc atcaccgtcc tgggcggttc cgggaccctg gtggggcccc tcctcggggc
     9121 ggcggtgctg atccccctgc aagagtattc ccgggcgctc tggggcggcc tggggggggg
     9181 cgtcgatctc atcatcttcg ggctcctgat catcatcatc gtggtcaagc agcccgccgg
     9241 gataatcggc atcatccgcg acatccggaa atatgccgcc cggcggcgaa tggccgggaa
     9301 agaggaggcg aaccatggcg ctccttgagg tgcgggaggt gacgaagaaa ttcggcgaac
     9361 tggtggccaa tgaccgggta agcttcaccg tggagaccgg gacgatcgtg gggttgatcg
     9421 gtcccaacgg cgcggggaag accaccctct tcaactgcat caccggcctc tatccggcgg
     9481 acggcggcgc catccacttc gacggccggg agatcacggc gctcaagcct tacgagatcg
     9541 cccgccgggg agcggtgcgg accttccagg tggtgcggcc cctcaaggaa atgacggtct
     9601 tcgacaacgt cctggtcggc gcgtttctca agaccggcga cagacgcaca gcagccgccc
     9661 gggcggagga atgcttgcgg atctgcaacc tggagccctt tcgggaccgt cctgccggag
     9721 ggcttcccat cggcggcaaa aaacgtctgg agatggcccg ggccctggcg acccagccca
     9781 aactcctcat gctggacgaa gtcatggccg gcatgacctc caccgagatc aagacctccg
     9841 tggaggtcat cctccggctc cgggagagcg gcattaccct catgatcgtg gagcatgtca
     9901 tggaggggat catgcccatc gccgacaagg tggtagttct cgacggcggg atcaagatcg
     9961 ccgaagacgc ccccggcaac atcgtcaacg acgggcgggt gatcgaggcc tatctgggcg
    10021 ccaaatacag ccagcgtttc aaggcgcgga aggagggtgg agaccttggc tgaaccgatt
    10081 ctgaaggtcg aggacctcca tgcgggctac gacgggctcc ccgtcgtctt cggcgtttcc
    10141 ctggaggtca tgccggggga gctggtggcc atcgtcggcg ccaacggcgc ggggaagacc
    10201 accacaatgc gcaccatctg cggcctcgcc catcccctca aggggaggat cgtttttaac
    10261 ggcacggaca tctccgggct gccggcccac cggattctga agctcggaat cagctacgtc
    10321 cccgaaggcc gccggctctt cgccaagctg tcggtggaaa agaacctcgc cctgggggcc
    10381 tacgccgaga attctcccgc ggaggtaaag cgccgacagg aggagatcat ggaaatcttt
    10441 cccatcctcc gggagcgggc ccgccagacc gccgaaaccc tgagcggcgg ggaacaacag
    10501 atgctggcca tcgctcgggg cctcatgtcc cgcccgcggc tcctcatgct cgatgaaatg
    10561 tccctgggac ttatgccaag tttggcggag aagatgatgg aaaccgtcgt tgccgtcaat
    10621 cgggccggca ccaccgtcct cctggtggag cagatggtcc aggagaccct ggagatcgcc
    10681 caccggggct acgtgatcca gaacggccgg atcgtccaat ccggcgccgc ccgggacctg
    10741 ctggactccg aaacggtccg caaggcttac ctggggatgt agttccccct cttcacaatt
    10801 ccggaattca ttccttcatc cgaaaaaccg tccggcgagc cagaaaaggg ccatgccgac
    10861 gagcaccgtg ccgcccagag actttgtctt cacggcaaag aggaaggtgg gaacggcaac
    10921 gatcagctcc gggcgggtga gatcgaggct gcggggatgg ccccccgtga tcagcgaggg
    10981 gagcagcagg gcgctcagga tcgaggcagg gatgaaatcg agccaggatt cgaaccaggc
    11041 ggggagcttc ttttccgcca ggcagtgcag ggggagccac cgggggatgt aagtcacggc
    11101 gcccatgccg gcaaaggtaa gcaggataag gccttcgtcc atcatggccc gttcctttcc
    11161 gcggtcgaaa ttctctttcc acctgccagg gggagacaga ccgtggcggc gaccagggag
    11221 gcgatcacga cgtagaggtt cccctccatg agcagggaga ggccgacggc caggaggccg
    11281 gcgacggccg cggccaggat atggagacgg gttcgcgttt gcatgatgag gaggcagatg
    11341 aacatcgccg tcagggcgta accgatgccg aaggccccgg cggggatgag ttccccggca
    11401 tagcagccga tgatcgtggc ggcgatccag gtgaggttgg agacggtatt tacgaccaga
    11461 gcccggcgcc agtcccagct tccggtacgg aagcgggtca tgttcacggc gaagctctcg
    11521 tcggtgacgc cgtaggcgta gagggccagg acgcctttcg ggagcccccg gaggtggacg
    11581 gcgagggcgg cgctcatgag gaggtggcgg aggttgacca cgaaggtcgt catgatgacg
    11641 gcgagggtcc cggcgccggc ggagagcatg gagatcccga tgaactgggc gctgccggcg
    11701 aacaccagca gggacataac gcccacgtcg agggggtgaa gacccgcttt ctgggccagg
    11761 acccccaggg cagcgccgat gggaatgtac cccaggcaga tcggccaggc ggcggcgagc
    11821 cccggccgaa tggcgtccgg ccggccctca gttgacgagg gtcccggtgg ggcgttcaaa
    11881 agccgctgcc gccgtgatcc agcctttgcc ggtcctgggc ccgttcgaaa ttggcctcgt
    11941 tccggtaggt ctcttcactg gatgccttgt cgcgggcggc atcccgacct cccgcgcacc
    12001 ccgcaacgga tagaaacata gcacccatga ggataacgag catgaacaac aagaatcttt
    12061 tcacctggcc gacccctttc ggatttcaat tatagtttgc ggaagtccgc ttcaaagccc
    12121 cttttctcgc gccgccagtt ctccggcaag tttaagtatc cacagcatcc acagggtgat
    12181 gacggcggtc aaggccatga atatggccgt cagcggatag tcgatctgga attcgaaggc
    12241 gttgaggatc cggggatcgt aacggcccgc atctaccagg aggagattga acttgtcccc
    12301 gagattgtag aagtagtcga acacattggg gggctgcatg atggtgtgta atccccagtg
    12361 cttgctgatg atggtgttgc cgatatggag agagcatttc caggggggat tgaaccccag
    12421 gaacaacaaa acggcgctgg aaattaacag gacggcgatc ttggcggcgg cgaatctcat
    12481 ggcgctcctt tatggtaatt gtttcaagat cttgcaggct tgatcgtagc cgaggcggca
    12541 ggaccgctcc gcgtcctggc gggcttcatt ttttttgccc tgctgatgat agcaccggcc
    12601 ccggtgatag taaccccagg cgttgtcggt tttcaaggca acggccctag tgagatggac
    12661 gacgcattca tcgtagcgct tctcctgttc ggagagccat cccagccagt tgtgggcctc
    12721 gacgtgattc ggattcaggg ccgtggtatg ggtgaagtcc gccgccgctt tgtcgggaag
    12781 gttcatcttg tagtaggtcc ggcctcgcca gtaataagcg gcgtcgttgt tcggatcggc
    12841 ggcaagggcc tgggtatagg cggcgagggc ccggggaaaa tcgttctgac ggaagaagcg
    12901 gtgcccggcg tccagactgt ctccgcgggc ggccggggcg gggcgcggcg tctccccccg
    12961 ctctttcccc ggggtcaggt tgcgcagcct cccctggcgg ttttcgtaca tggccgcgac
    13021 gatttgccag ttgttgacat agacgagggt cacatacgcc ttgcccgtcc cgttttggcc
    13081 tttgatgcgc agggtcagct tgacgtgggc ggtgtttccc gccgctttga tggagaccgg
    13141 aaaccagggg tatatttcct ttatctctcc cagatcctcc cggatcgacg gattttcctt
    13201 cacgaaggag gaggcgatct tgaaaggttc cgaatccctg acaaattcat ggagggcgaa
    13261 aacggcggcc agggcgatga agaccagcat gaacggccag gtccgggccg atcctccctt
    13321 cggccggatc ggctggtagt ccttcttccc acttccgggt tgaattggtt cgtaggcctt
    13381 ttcgctcccc ggaccgggcg cccgatcttt ccgcatatac tgcgtcagga gatccggcag
    13441 tttcatgttg ggcaaggtgg tcatgatcta tccccccagt tttcatgacg tgcgccgtca
    13501 ttatagaggg aaagattttt ctgtcaagct aaaccccgtt gcccggggtt tggctgtcca
    13561 tgagctcgct gccgctcctt gcccggaatt cgttgacccg gaaggcaagg atggtgatgt
    13621 cgtcgtactg cggggcgtcg cggcagaatt cccggacgtc cgcaaggagg tcgcggcaca
    13681 acgcctgggg atctctgtct tgccgtccgt cataacggca ctgcagccgc tcgtccccgt
    13741 ataggcttcc ggatgaggtc tgcgcctccg tgacaccgtc ggtataaaag attacggcgt
    13801 ctcccggcgc catggtgaaa ctccactcct ggaaggcggc gtccgggaaa ggaccgagaa
    13861 acaggtcggg ttgggtgtcg aggcgccggg aggccccggc ggagcagaga agcggcgggt
    13921 tgtgaccgcc gttggccagg accattcgcc ccgtccacag atcgtattcg gcgtaaatga
    13981 cggagacgaa catcgaggcg tcattgtcgg cggcgagcat ccggttggtc ttgcccaggg
    14041 tgcgggccgg gccttccccg gccagaccgt aaaccctgac cagcgcccgg gccatggaca
    14101 tgaacagggc ggcggggacc cctttgccgg agacatcgcc gataacgatg cccagcttgt
    14161 ccgggtcgat gaagaagaag tcgaaaaagt ctcccgccac ctctttggcg gggatcatcg
    14221 ttccgtagat gtcgaactcc cggacgtcgg ggaagggagg gaaggtatgg ggtatgatgg
    14281 agtgctggat ctcacgggcc agttgcagct ctccctccat ccgttccttg acggccgtgg
    14341 tctccgtaag gtttttgatg taggcgctca gatcggcgga catcttgttg aggacctccg
    14401 ccaactcctc catctcgtcc cccgtgggga tatggagccg ggattccaga tctcccgcgc
    14461 cgatccggcg ggcgtgaagg gcgactttat ggatgggacg ggaaaagctc cgggcgatga
    14521 acaccgacac gaggatgagg accacggtgg ccaggaagga ggaaatgagg aagatcctta
    14581 tggatgcctc cttttccctg gtgatggtct cctgggcctg atacaggggc tggtaaaatt
    14641 catcgacgta aaccgtggca aaaaggatgc agtcggtccc ccgcacgggg atgccgacga
    14701 agaatttttc ccggaaggtg ccgtcctttt cctcccagag gtagtaacgg ctcacgggcc
    14761 gcccgttcag gttctcccgg atggcctccc agagggcggg gagcttgtag cgaaatgccg
    14821 acaggtattg cccctcgatg gcgggattgg ggtggaagag atacttcccg tcctgtttgc
    14881 ccaccccatc gtggagaccg ctgtagccgg tccgctggat tttctggacg gcgatcttcc
    14941 tgagttccgg gtcgtggata tcaaaggggt ggtaccgcag atatatctcg atctgtcccg
    15001 ctagggcgta ggccttttcg ctgaggaatt tctccaatcc cccgcggatg gcggattcca
    15061 tctttaccgc gttgtccctg gcgttttcgg tgatgcggac cacgccgatg aaggagaaaa
    15121 gggtcgaaaa aaagatggcc agggcgccgt aaacgagaat gagtttggtg cggatggtca
    15181 tggaaagcgg gctcacaatc gattcttgcg aatacatcgt tgagaccatt gaaaaatagc
    15241 ttcgccgcgg ctcaagaatc tttttcgctt ccagtcagta tgcatatact tatgattaat
    15301 tacgagatgt taagtctaat ctcggaacgg cgaataacat ttcattcgct gcttgcgaac
    15361 cattttccgc ggttatgaaa agctctccgt tttcaattga ttttatggat gccggccccg
    15421 gaaaagtcaa tcaaaaagat ctgttggcaa tggcctcttt tgaccccggt ccgggttttt
    15481 catcccatca aaccgggcag ccagagggcg atttgcggaa atatgatcag gagcgccgtg
    15541 agaatgatca tggcgcacat caggtaggcc gaccccttga agatgatatg cagcggcacg
    15601 tcccgggcga tacccgccac cacgtagacg tttattccca ccggcggggt tatgaccccc
    15661 atgccgccca ccatgacgat tatcaccccg aaccagatcg gatcgtagcc cagggtggtg
    15721 accacgggat agaagaccgg gatcgtcaac atgatcatgg ccagggagtc catgaggcag
    15781 cccagaaaga gatacgtgac gatgatcagg gccatgacca ccacgggggg cacggggagt
    15841 ccggccaccc aggcgccgac ttccgtgggg atggtcgtaa cggccaggaa atgaccgaag
    15901 accgtggccc cggccacgat ggtcatgatc atgcaggaga tgcgcaccgt ttcgcttagg
    15961 gagcggacga aggagcgcca ggtcagattg cggccgatca gggcgatgac ataggtcccc
    16021 agggcgccga tacccgccgc ctccgtgggg gtgaaaaggc cgacgaagag gccgcccatg
    16081 acgatgaaga acagggcgat ggtctcgatg accccggcca gggaggcaaa tttttcccgc
    16141 caggaggctt tgggggccag ggcgcacagg ccgggctgga ggcggctcca gaccaggatg
    16201 accagggcga aaagggcggc caggaggatt ccgggcagga ccccggcgac gaagagcttg
    16261 cccacggact gctccgtcat gatcccgtag atgatgaaaa tggtgctggg ggggatgagg
    16321 ataccgaggg tgcccccggc ggccacgacg cctgtggcca acgccgggtc gtagttgaat
    16381 ttcttcatct ggggcagggc caccgtggcc atggtggctg aggtggcggt ggtggagccg
    16441 caaatggcgg caaaaaaggc gcaggcaccg atcgtggcca tggccaggcc gccgggcagg
    16501 tgaccgatga atttatgggc ggcgttgaag aggcggccgc tgattcccga ataatgggcc
    16561 acctgaccca tgaggataaa gaggggaatg acggtgaggt tgtaggagct gaaaacggag
    16621 aagaaatccc tggccagcag gctcagcgcc gccggccagg agacgagcca cccgaagccg
    16681 acgaagccca ccatggccat gacaaaggcc acggggatgc ggaggaacat gagcgcgatg
    16741 agtaacgcga acccgacgat cccggcgctg atgggcgtca tgaaccgatc ctccggaggc
    16801 cgcggccgaa ttcggcgcca aggaccaggc aaagcagggc gcagcccgcg gcgatcccgg
    16861 cggcgaaggg atagacgggc atcccgaggg tggcggaaac ttcgccgctc tgccggaggt
    16921 ccagggcgta caggacgctc tgccaggtca gggcggcgaa gagagtcaat ccgaagaggg
    16981 aactggcggc gtccagaagg atttgccatc gttggggcag gcggttgacc agcaggtcca
    17041 cggcgatatg acctttttcc atggaggtgg cggccagggc gagggccacg gccagggcgc
    17101 cgagaaatcc gatgatctcg taggtgcccg gaatgggcag gtcaaaaaac cgcatcacca
    17161 cgtcggcgca ggtaaggagc atcatcgaga tcaacgccgt cccggcggcg aggccggccg
    17221 gcctgctcag attgttgacg aacctttcga tcctatgcat cgtttcctac ttgtatgtct
    17281 tcgcgtattt cttgatcagc cgttccgttt cggccactgc cttctgaccc gggaggccct
    17341 tggccgtcgt ggtttttata tattcgttga gcatgggacg gacagccttt ttccaccggg
    17401 cgccctcctg cttgctcaaa gggatgatct tgttgcccag actcagggta tagcggcggc
    17461 cttcttgatc gaggtcatcc caggccttgc cgtggacatc gatccactga ctgctcacct
    17521 cttcaaagac cttctggatg tgcttgggca gggcgttcca tttcttgaga ttcatgacga
    17581 cgaacatggc ggtggtgtag ccgatctccg gacagtcggt ggtggatttg atgacctccg
    17641 cctgtttcca gccctttagg acctcgatgg gaccgaacgt tccttcgacg accccttttt
    17701 gcagggattc ataggtggaa ccctggggca tggcgaccgg aaccgccccg agcgaggaaa
    17761 cgaccttggc gctgagaccg gtggaacgga tcttcatccc cttgagctga tcgagggtgg
    17821 cgacgggacg gttggtgtgg aggaggcccg ggccgtgggc atgaagatag agcaccttga
    17881 ctcccttgag ttcctggggg ttgaagatcc ggaaatactc gttggccacg gcggtggcga
    17941 cgcgcccgtt gggatagccg atggggagat cgaggacctc catgacggga aagcgccccc
    18001 gggtataagc gaagcatgac atccccagat cggagatccc cttgagcacg ccgtcataga
    18061 cctgatccgc cggggttagg gaaccaccgg caagaatggt tatcttcacc tgtccctggg
    18121 tgcgcttttc aatctccttg gcccaggctt cccccgcttt ggcctgtccg tggctgggcg
    18181 ggaaaaagat gctgtaggtc agcgatgtcg cggcttccac cgcgccggca ctgaatgtcc
    18241 atactaatga aatgattgat aaagccgtga tgaactgttt cttcatgctc ctcctcctct
    18301 cttttcttgg acgggaagag tcttcacttc ccgtctgccg accggacgca ggtcggcggt
    18361 agggttaaat gacgtttaac tgacgccggg catatatgga tgaaaaaaac atctcgtttc
    18421 ttgattcctt attgtcttta tggggattcg gttggaaaaa atattgggag cggcctgcct
    18481 tcccgtcttg cgacggtaag caggcatcaa tacatatagg tgtgtctttg gaggcgaaca
    18541 gtggatattt gcatgggcat taggtgcata tacggttctt tccaaagtaa aacttgggaa
    18601 ctgatagggg aaataacctc ggctgtcaag gattattttc ctttgatttc aacgtttcga
    18661 cccgcaggcg ttccagctct tcccggtaaa gctccgcctt ggcccgaatc tgctcgatct
    18721 cttcctgggc cttttccagc ttgtcatcct gccgcttttt ttcctcgcgg attctctcct
    18781 ggatgtcgtc gtagcccacg tacatggcca gaccgccgcc gaggatcatc aggatgggca
    18841 acgccccttt gacgatgacg gcgaaggagt tccaccagac gaccaatccg atcagaccta
    18901 agatggcggc gacggcgccg cccacgagca ggggcattaa aaggacctcc ttgactaacc
    18961 atggaaatga gaatggaagg aaaagtacca aaaaaaaaat ctcctggcaa gacgaaaaat
    19021 gcgttgggga aaaaaaggct tgctaaaata ggcacgttct attaccatcc ccgcccaatt
    19081 cagtaaagaa ccggagcggt tattttccgg gaaacgagga gacaaatcta atgatagtgc
    19141 cggattttaa tcgggccggc ggtttggtgc ccgccattgt tcaggatgat gaaacgggcg
    19201 aggtgctcat gctggcctat atgaacaggc agtcatggga aaagacactg gtgacgggtc
    19261 tggcgacctt ttggagccga tcgcggcagg cattgtgggt gaagggggaa acctcgggaa
    19321 acgttcagga gatcaaagaa atccggatcg attgcgatct ggatgccgtg ctgctcaagg
    19381 tccggcagcg aggcggggcg gcctgccata cgggataccg ctcctgcttc tatcgccgga
    19441 tcgccgccgg cgggctggag gtgtgcggcg agaagatatt cgatccggag gaagtctata
    19501 aatgaatacg ttgcggctgg gaatacccaa gggctctctg gaggccagca ccgtggacct
    19561 gttcaagcgg gcgggatggc gcattactta cgacagcagg aactatttcc ccgatatcga
    19621 cgacgaggag ataacctgca tcctcgtgcg ggcccaggag atgtcgcgat acgtggaaga
    19681 gggcatcctc gatctggggc tgacgggtcg ggactggatc gtggagaacg attccgatgt
    19741 ggtgacggtg acggacctga tctattcaaa ggtgtcggcg cggcaatcac ggtgggttct
    19801 cgtggtcagg caagattcgg agattcaatc catagcggac atgaggggaa aacgtatctc
    19861 caccgaactg gtcaatttca ccaagaatta tttccgggaa cgggggatcg atgtccacgt
    19921 ggaattttcc tggggggcca cggaagccaa agtcgtggag gggctggtgg acgccatcgt
    19981 tgaggtcacg gaaacaggat cgacgattag ggccaacaaa ctcaagatca ttcatgaatt
    20041 gatgaaaacg aatacgcagt tgatcgccaa tcgcctcgcc tgggaagatc cctggaagag
    20101 gcagaagatc gatcagatca agaccctcct taccgggtcc ctccaggcga tggggaaggt
    20161 gggcctgaaa ctcaatgtcg ccaaggagaa tttgggccgg gtgatcctgg tgctgccgtc
    20221 tttgaaggcg ccgacgattt ccagccttta ttccgaagaa tggtatgccg tggagacgat
    20281 cgtggacagc gccatagtca gggacctgat tcccattctc caggaggcgg gggccgaagg
    20341 aatcatcgag tacgccctga acaaggttat ctgacccttt ccaaatcaca gggaggacgg
    20401 aggatgcgca agggcaagat caagagaaag accagggaaa ccaacatttc cgtggagatg
    20461 gaactcgacg gcgtcggccg gggcgagatc gacacgaaga ttccgttcat ggatcatatg
    20521 ttgaatcttc tggctcggca tggtcttttt gacctcaaga tctccggtca aggagaccgg
    20581 gaagtggatg accaccatct ggtcgaggat ttgggcatct gcctggggaa cgccgtgaaa
    20641 aaggccctgg gtgacaagga ggggatcgcc cgctacggag cggccctggc ccccatggat
    20701 gagagcctgg ccagtgttgc cctggatatt tcgggccgac cctgcctcgt ctatcatgct
    20761 gagttggggg aggcccggat cggggccttt gacccgtctc tgctccggga gtttttcaag
    20821 gccttttccg atcacggtgg aatcaccttg cacatcaatg tcctctatgg taagaataac
    20881 catcacatgg cggaggctgc attcaaggcc tttgccttgg cactgcgctc cgcggtgcag
    20941 ctcgatgaac ggattcaggg cgtcatgtcc acgaagggtc tgctctgaac ttacttataa
    21001 aacccctctc tttaagcttg catcggccca tgaacaatta catttcagcg aataaaatct
    21061 cgtatctccg gaaatcatcc cctccatctc cggcaaacca tgccgccgcg ccgggacgga
    21121 atcaccggcg ccgggggttc aaggcggctc tttccctgct tttgtgggtc ctgctcctgg
    21181 cgacgacggg ctgccagagc gcaagcgggg tttcgacatc actgttgcgg gaccgggaca
    21241 tcgtctttaa acgcctggcc gtcatgccct tccagcaggt gtctcccgag atcgtggcga
    21301 cttattccgc ccgcagttcc cttccggcct ccgtgctgaa gacggacaac gacccccgga
    21361 gtccggaacg gtatctccag gacctcttca tggaattgat ggcgaaacat ccgcagttcg
    21421 atctcgtttc cccggatcgg gtcggcggca cctacgaagg cgtgagcacc ggttccctca
    21481 aggccaccct gcctaaggcc ttgaaggaag cagggacgga actcggggcc gacggcttgg
    21541 tcgtcggtta tctctaccgg tggagggaac ggcgtggtta cgattattcg gtggaaaaac
    21601 cggcctcggt tttttttgag atccagttgt accggaccgt ggatgggatg atggtctgga
    21661 agggggtctt cgacaaaacc cagaagtccc tcatggaaaa tgtcctgggc gcctcctatt
    21721 tcgttaagga tcgagggcgc tggctgacgg cgaaggagct ggcggcggag ggaatggccg
    21781 atattgtcgg cagatggccg ggggtcgcca agaaggaagc gaagaaggcg gagtcacggc
    21841 cggagtgacg ggtacccgga accgcggccg ttttcccttc ttgatcgaag acggtgcggc
    21901 cgcggggaag gaaaggacat ccggatgacc gtttgccccg tctacgtggc caaagatcgg
    21961 aattccccgg gggaattccg aaattttttt ccggggtggc aatgattgta attcctgcga
    22021 tcgatctcaa ggacggacgg tgtgttcgtc tggctcaagg tgatttccag cgggtgaccg
    22081 tttatgcgga gcaccccgcc tcagtggctg cgcagtggca ggaacagggc gcggagcgcc
    22141 tccatgtcgt cgatctcgac ggctccctcg ccggatcgcc ccggaaccgg gaggctatcc
    22201 gcgccatcat cggcgcgatc gaaatccccg tggaattagg aggcggaatc cgtgatctgg
    22261 agacggtggc cgattacctt gacctcggcg tccgctgggt tatcttgggc acggcggcgc
    22321 ttagaaaccc cgggctggtg cgggaggcct gtcggacctt tcccgatcga gtcatcctcg
    22381 gcatcgatgc ccgggagggt ctggtttcca tccagggctg gacggaaacg accgcgcatt
    22441 cggcggtgga attgattcgg tcttatgtgg attgccgcct gtcggcagtt gtttacacgg
    22501 acataaaacg ggacggtatg gaaacggggg tcaatgtcga cgccacgcgg gccctggccg
    22561 aagccaccgg cgtgcccgtc atcgcctccg ggggcgtgag cggcatggcg gacatcgaaa
    22621 aactcatgga ggtggccccg tcggggatca tcggcgtgat tgccgggcgc gccctgtaca
    22681 ccggcgccct gtccctggcc gcggcggtcc gccaaacgaa ggccggacgg aacggttgac
    22741 agcaggagcg catttacggc ccgttagctg tttttataat gaactcaatg gttaaagagc
    22801 tcgcaacagg agggaggaac gatggacgac tgtatctttt gtaaaattat caaaggggag
    22861 ataccctgtc gaaaggttta cgaagatgaa tcggtgctgg ccttcgacga catccatccc
    22921 atggcgcccg tccatgtggt gatcatcccc aagcgacacc tggccacgct tctcgatgta
    22981 ggggaagaca cactgcccgt tgtggacgcc atgatcaggg tggcccagga agtggcccgg
    23041 aggaagggca tagacaagcg gggattccgg acggtgatca actgcaacga agaaggggga
    23101 caggtcatct tccatctcca catgcatctg ctgggaggga gaaaactggc ggacgggctg
    23161 ggataaaatg gcgtttctcg ttgacaacag ctgaagggtg gataacttag agcagcgaaa
    23221 tctacaatcc atatttgagg acaggaagag atggaactta agaacaaact ggaagaggaa
    23281 ctggtcaagg cggccaaggc caaggaaaag cttcgcctgt cggccctcag attggtcaaa
    23341 aacgctattc acaacaagga gatagatatc aaacgggaga cgacggaggc ggaattcctc
    23401 cagaccctcg cctccatggt gaagcagcgc aaggattcca tcgaacagtt cgaaaaggga
    23461 ggaaggaccg acctggtcga aaaagaaacc gccgaactca aggtgatcca ggagttcatg
    23521 cctcggcagt tctccgagga ggatctcgac gcggccatcg aaaaagccat ccgggagacc
    23581 ggagcggcgg gaccgaagga catgggcaag gtcatgaagg ccctgatgcc cgtgatcagc
    23641 ggccgggccg acggcaaggt ggtaagcgag aaggtcaagg ggcgcctggc gggttgatgc
    23701 cgtcccatga tgcaaggctc gattcctgaa gacaagatcg acgaaatcaa aagacggatc
    23761 aacatcgtgg atctggtggc aggttacgtg aacctgaagc aggcggggcg gaatttcatc
    23821 ggtctgtgcc cgtttcatca ggaaaagacg ccttctttta cggtcagtcc ggaaaagcag
    23881 atcttttact gcttcggctg cggggaaggg ggaaacgcgg tcaccttcat catgaagcaa
    23941 aacggcctct cctttcccga ggccatcagg tatctggcgc ggaaaactgg cgttgccctt
    24001 cccacccgtc ccctgagcaa taaggaaaag ggggagctgt ccctacggga aaaaatgctc
    24061 cggctgcacg gactggctgc ggcttttttt tccgagcgcc tggaggggca agaaggtctc
    24121 gcggcccggc aatacctgcg ggagcggggc ataagtgcgg cggtcgtaaa ggaatttcgc
    24181 ctgggttatt ccccgccggg ttggcgtcac ctaaaggatt atctggagcg gaagcggatg
    24241 ccccttgacc ttgccgagca ggcgggcctc ctcgtgaaaa acgagcgggg ggatttctat
    24301 gaccgtttcc ggggccgcct tgtctttccc atcgaagatc agtccggcca gatcatcgcc
    24361 ttcggcggcc gcatcatggg ggagggaatg cccaaatatc tcaattcccc cgaatcggcg
    24421 ctcttcagca aggggaagaa cctttacgga cttgtcaagg cccgggagga gatccggaaa
    24481 agcggttacg tgatcctggt ggagggttat ttcgatcttc tcgctctctg gaacgccgac
    24541 atcaggaatg tcgtggccac gctgggcacc gcccttaccc gggaccatgt ggatatcctg
    24601 cgccgatatg ccccggaggc ggccgtcgtc ttcgatggcg atgaggcggg gaaaaaagcc
    24661 ctggcccgga gcctggcgct gttcgtcgag ggcaacctcc ccatgcgggc ggtgatgctc
    24721 cccccggccg ccgatcccga tgactacgtc cgcggctccg ggagggaggc cttcctagcc
    24781 ctggtggcca cggcgccccc agcggtggag tattacgtgg ataacttcat cggcaccccc
    24841 gagtccatgg agggaggccg ggaagccctc cgtaccgcca tggcctttat cggaaagatg
    24901 gagagcgatg cggagagaaa tctcttcatc aagcgcgtgg cccagcggct cggcgtcgat
    24961 gaggatgtcc tcaaggcaga agtgaaccgg ggacggagaa agaagacggc ggagccggag
    25021 gcgggggaaa agaccttacc ttccaccgcc ggcggggatg ccctggaggc gagcttcatc
    25081 gccctgcttc tcgaccatcc ccgtatgatg cccgccgtta ttaccggcga agccttgacc
    25141 tacctgcaaa acgaggagtt gaaaagactt ggaacggcgg cggtggcgca atatgaacgg
    25201 caggggggga tcgatctgga ggattttgtc gcccgcctca gtcccggggc actgagagag
    25261 gcgattttgg gccggctggt cgattccgtg tcctgggagg gggaaacagc ggaccgggta
    25321 tgccgcgaca tgatccggca gatcaaacgc aggtggttca aggagaagcg ccgatctttg
    25381 aacgtgtccc tggtccgggc ccaacaggcc atggacatgg aatcctgccg gcggctgctt
    25441 gccgaaaaga cggagcttat tgaaaaagag aaatccatgc tgtcgaatgg ctaaggtgga
    25501 ggatttttat gggtaaagac atccattccg aggaattcaa gaaactgatg tccctgggag
    25561 aggagaaagg ctttttgacc tatgacgacg ttaatgacat gctgccgtcg gacatcatct
    25621 cttcggatca gattgacgat atcatcatgc ttttcgggga gaagaatatc gatatcatcg
    25681 atacggacaa aggtgaaaag ctggtcgtga aaaagtctcc cgaggagctg gtaggggcta
    25741 aggacgacga tctcctcggt ttgatgcccg tggcgggaaa aaccggcgat ccggtcaaga
    25801 tgtatctccg ggaaatgggg atggtcgcgc tcctcagccg ggaaggggag gtggagatcg
    25861 ccaaaaggat cgaagaaggc attcgggata ccatggactc cgtcttcagc gtcaatgtct
    25921 gtgtcaagga ggtcatggat atcgggaaga agctccgcgg cggcgagatg cggatcaaaa
    25981 ccgtcgtgga caacctggaa gacgaggacg gcttcgtcga ggaggatttg caccgcgatc
    26041 gggtcatccg tctgatcgat aggattacgg ccttgagcag aaagaacgac aagatcaagg
    26101 aggcgctgaa ggaccggagc atcacgcccg agcagcgcgc cgtccatgag aagaacctgg
    26161 agaagaatgt caaggccatt atccgggatt gccgaaaggt caagttcagc aagcggcaga
    26221 tagaccggat ggtgtccatc ctcaaactct tcatcaacga agtggataag gcggatgaag
    26281 aaatccggcg ctgtaaagaa gacacctgtc tgtccctgac ccagttggaa aagtcctgga
    26341 gccgcctgcg ccaggaccgg aagacccaga tgcagatggt caaggagctg ggcgtgccgc
    26401 tggataaact aaaagcggcg gagatcgtca tcaaggatgc gaatcgccgc aagaaaaagg
    26461 tggctcagga aaccggcctg tcggtgacgg agctgaagaa ggtggtggcg accgtcgagg
    26521 aaggggaagc caaggcggaa ctggcgaaaa agaccctggt ggaggcgaat ctgcgcctgg
    26581 tggtgagcat cgccaagaaa tacaccaatc gggggctgca atttctggac ctgattcagg
    26641 aaggtaatat cgggctgatg aaggcggtgg ataaattcga atatcagcgg ggctacaaat
    26701 tctcgacgta cgccacctgg tggatccgtc aggccatcac ccgggccatt gccgaccagg
    26761 cccgtacgat ccgcatcccg gtccatatga tcgaaacgat caacaagctg atccgcacct
    26821 cccggtacct ggtccaggag ttgggaaggg aacccacccc ggaggagatt gccgccaaga
    26881 tggaattccc cctggagaag gtgcggaagg tcttgaaaat agccaaggaa cccatatcac
    26941 tggagactcc gatcggtgag gaagaggaca gccatctggg cgatttcatc gaggataaaa
    27001 agataatgtc cccttccgag gcgacgatca gcatggatct ggcggaacag acccgcaaga
    27061 tactggcgac tctgacgccc cgggaagaaa aggtgttgcg gatgcgtttc gggatcagcg
    27121 agcgggtcga tgtctccacc gatgaaatga aggagacgcc ggtgagtttg tctctggaac
    27181 gggcacgtca tgtggagaat aacggcgtcc gcaaactccg taatccctcc cggcggaaac
    27241 tgatccggcc ggcagggtga ggatggcggg cgggatcgtt gcgccaaata agttattgac
    27301 atcccatcgg cttgagtata tctaagcagg tcattgtgga aaggagacag ttgcgggccc
    27361 atagctcaat tggtagagcc accggctcat aaccggtagg tccctggttc gaacccaggt
    27421 gggcccacca taatatatgt atagcgatta caaagaattg cggataccaa cttacaatgg
    27481 gatgacagga agcggcgtct tcccggaaag aaaagtgcac cttgcaggag gtgcactttt
    27541 ttttgtcttg tttcttgtga gaaaggggta aataattgaa caaaaatttg atgcatctca
    27601 tcgcgttgca gaagatcgat acggaaacgc agaaagtcct ccagagcagg aaagatcttc
    27661 cactgcggct ggccagactc gatacggact ttgccgccgt ccgggaggcg ctggaggagg
    27721 aaagaaaccg gttggaggcg aagaaccaaa cccaccggga actggaagag gccctcaaga
    27781 aaggtatgga gcagttgcgg aaaacccgag accgcatcca cgaggtgaaa tccaacaagg
    27841 aatatcaggc ggtgctgaaa gaaatagaga ccctggaaca gaaacacggc atagtggaag
    27901 acaagatcat caccattctg gaagagatcg acaccatgaa gacttccctg gcgatccggg
    27961 aaggtgaatt ccaggcgtcc gggcggcgat acgaggagga aagggaaaag ctggaaaagc
    28021 gcatcggttc catcgaatca cagctccagg aacggcagcg ccaaggggag tccatgcggg
    28081 aaaaaattcc gaagaatgta ttgaaaagat atgatatgac taaaaacgtc aatcagggcc
    28141 tggcggtggt ttccgcctgg aaggaaacct gcgacggctg tcacatgagc attccccccc
    28201 aactctacaa cgaattgctg gaagatacgg aagagctcat aatgtgtccc aattgccgcc
    28261 ggattatcta ctggtatgac cagaacaagg aaagtgtttg aggaggacag atgatcgctg
    28321 gtccggcaag tccggacggg aggaaagtcc gagctccgta gggcaggatg gtcgctaacg
    28381 gcgactgggg gcgaccccaa ggaaagtgcc acagaaaata taccgcccgc gttccggagc
    28441 gccgctccgt ggcgggtaag ggtgaaaagg cgaggtaaga gctcaccgct ccgctggtga
    28501 caggggaggc atggtaaacc ccatccggag caagaccgaa taggagaacg atcgagggcg
    28561 gcccgcccga ggttctcggg taggtcgctt gaggcgtcag gcaactgccg ccctagagga
    28621 atgatcatcg ccggcggccg ggggtgaccc ggagccggaa cagaactcgg cttacggcct
    28681 cctcaaacat ctcataaagc aggggcggtt gccgtcagac ggcaaccgcc cctgctgtta
    28741 atatttcccg cccccatatt tcccgctccg taaacttccc cctacggttg aaacttgttt
    28801 gacgggggac ggaaacattt tacgccttat ttcctcagcg tcctctccgt ttccttgatg
    28861 tcgatggtgg tcttaatgac cgtatcggaa ttcagggaga tgctgctgat cccgcattcc
    28921 actaggaagc gggcgaattc gggataatcg ctgggggcct ggccgcagat gccgatcttc
    28981 ttgcccatct tgttggcctt ggtgatggcg attcggatca gatccatgac ggcctggttg
    29041 cgctcgtcga agatatggga aatgaggaag gaatcgcggt cgagacccag ggtgagctgg
    29101 gtaaggtcat tggagccgat ggagaagccg tcgaagatct tgccgaattc ctcgatgagg
    29161 atgacattgc tggggatctc caccatcatg tagatctcca ggttgttttt gccccggacc
    29221 agcccttttt ccgccatgag ggccacgacc tttttccctt cttccacggt gcggcagaag
    29281 gggatcatca gtttggcgtt gacgaggccc atctcgttgc gcaccttctt catcgcctcg
    29341 cattccaggg cgaaggcgtc acggtatcgc tcgtcgtaat atcgggaggc cccccggaag
    29401 ccgatcatgg ggttttcctc atccatttca aaatactggc cgccgatgag gttcttgtat
    29461 tcgttgctct tgaaatcgct catccggacg atgacgtcgt tgggataaaa ggcggcggcg
    29521 atcttggcca ccccctgggc caacttgtcc acgaagaaat cggcccggtt gtcgtagcca
    29581 tgggtgaggg cggcgatctt ttcccggatg gccggatcag tgaccttgtc gaattggatc
    29641 agcgccatgg ggtggatgct gatgaactgg ttgatgatga actccaaccg ggccagcccg
    29701 acgccgtcgt tgggaatggc ggcgaggttg aaggcgtttt ccggattgcc cacgttcatc
    29761 atgatcatcg tcttcgggcg ctcaagattc ttggcatcca cccgctggac gtcgaatttc
    29821 aggagacctt catagacgtt accgatctcg ccttccgcgc aggacacggt ggcttccttg
    29881 acccccttga gggctgtggt cccgttttcc gtgccgacga tgcagggtac accgagttcc
    29941 cggctgacga tggcggcatg acaggtccgg ccgcccttgt tggtgacgat ggccgcggcg
    30001 atcttcatga ccggttccca gtccgggtcc gtcatgtccg ttaccagcac ctcgcctttc
    30061 ttgaagttca ttatctgttc cacggattcg atgatgttcg ccgggccggc gccgatcttg
    30121 gagcccaccg cggtgcccgt aaccagggga ttcttatgtt ccaggaggat gtagttttcc
    30181 aggacgctgg catccttctg ggactggacc gtttccggcc gggcctggag gatgaagagt
    30241 tcccccgtcc gtccgtcttt gccccattcg atgtccatgg gcttgtaata tccggcctgc
    30301 tgggagtaat gatcctcgat gatggtggtc caccgggcca gttgcagaat ctcgtcgtcg
    30361 ttgagacaga acttgcgtcg ctgctccacg ggaaccgaca ccgtctcggt ggcctctccg
    30421 ccccggcgct ccgcgtagat catcttgatc tccttggtgc ccagtttctt ctggacgatg
    30481 ggccgatatc cctgtttcag ggtgggtttg aagacgtaga attcatcggg gttgaccgtg
    30541 ccttgaacca ccgtctctcc cagtccgtag gccccggtga tcaggaccgc ttccttgaat
    30601 cccgattccg tgtcgatgga gaagatgacg ccggaggcgg cgagatcgga acggaccatc
    30661 ttttggacgc ctatggagag ggcgatggaa aggtgatcga aacctttgtc cacgcgatag
    30721 gagatcgcac ggtccgtgaa gagggaggcg aaacagcgtt tgcaggcctc gatcagggct
    30781 tcgtccccgg tgatgttcag ataggtttcc tgctggccgg cgaaactggc atcgggaaga
    30841 tcctccgccg ttgccgagct ccggacggcc acatcggtgt tttccccgta ttcttcgcaa
    30901 agcttcctgt aggcggtgat gatggcggcg ctcaggtcat cggggaaggg ggcgttgtag
    30961 atgagctccc gcaccttgtg acctctggtt ttgagatttt cgatgttgtg ggtgtccagg
    31021 tccgaaagaa ccgttttgat ctgatcgatg atccccgcgg attcgagcag atagcggtag
    31081 gcgtaggcgg ttaccgcata tccgtcggga atgctgaccc ccttggggat caattcccgg
    31141 cgcatttctc ccagggaggc gtttttccct cccacctgag ggatatcggc gagttgcaat
    31201 tcatcgaacc agattgtcaa tctgtcgtcg ctcatggaat tcctcctgtg tatagatgtt
    31261 tgtcgaggca ttaagaaaag actcgagaac atttatcaat aatgaataat gaagtcaaac
    31321 ggaaagatgt tcgatccctt ggttgaggaa aaaaaaggcc ggatcgaatc cggccttttt
    31381 cgttaagagg ttctatttat cttcttcctc gtcatcgtag aatgggcctt ccccctggga
    31441 gtcggccggc acttccgccg ctatggagcg ggagatgccc cgggagagca atccggcgat
    31501 ggcgaacagg aggatgatgg agatcaccag gatcagaatg atcgtggtgg accaggcttt
    31561 cgcctccttc tggatattct gggttgcctg ggcggcagac tcgttgaatt tttccacatc
    31621 tacccccatg ccgatccagc cgaatcctcc cggcgccgga taatcggggg cgaagaattt
    31681 gatcggcgca tacgcgacga acttggtgtg tccggcgaac ttataggtct tgatccccgg
    31741 ttttcccgaa gcggcgtcct tggcgatcaa gggcagattg ggatcgacaa aacccattag
    31801 attcagattc agcacctctt cgcctttttt cgtaagttcc gccgagctct gttccgtcag
    31861 ggccggtacc ggcttcccgt cggaataaag tccttcgacg tgataatcgt tgggatggga
    31921 aatcatcaaa ccccgattgt ccaccatata ggcgtaattc ccggtggcgg cgtccgcttc
    31981 gaataccggc gtggcctggg tgggaatcac atgatccgtg taagcggcga gatggcgata
    32041 gtcaagggcg agttgaacga cgccctgaag accttcccgg ccgtacagcg gggtagcgaa
    32101 acgaataaca cctgagaatc tcttgccttt ttcgaaatca gcccgatttg cataccagcc
    32161 cgtaaccggg gatacaaata catcaccctt gttcaaggcc ttggccgaag cgaaataggt
    32221 ttccgattta taggtggtgt tggccggaat gcccacattg gtcagccggg atttgggggc
    32281 gatttctcca ttggtgatct tgatgacctc gttgccgctt ttgtcaatga gggtcatctc
    32341 cgtataaatg gggacggaaa tctgtttcaa cttatcgcca tccttgatcc agacgttccg
    32401 ccggttctcg ctgacaaact gtttgtacgc agattcggtg gccgggatga tcgtggcgat
    32461 gaggacgtct ttctttcgct ccgtaaggaa actcgccact tcatctgcga tgttcatggc
    32521 ccgtatcttg atttcttcct gagatttctg gtccagaacg gtcacagccc gctcttttac
    32581 tgtaacccct aatttgaaaa tcccattggc gatgaggatc gccatcagtg ataaggggat
    32641 tacgataagc aggaagaata ccctcgcgac agtgaaaaac tgcttatctt gtttcttctc
    32701 tgacattctt tttcccccta actgtagttc attttgaagt taatttcatc ggtcggatta
    32761 acccaaaaag tactttacgt aggggttagc atagagaaga accagcgcca caaccagggc
    32821 ataaatacaa acggattccg ccatggccat accgacgagc atcacggtca tgatctttcc
    32881 ctgaaccccg gggtttcttc cgacggcctg acaggcccca ctggcggcat tgccgatacc
    32941 ggtgccggcg ccgatcgcac cgaggccgat ggcaaatccc gcaccaatca tcgcgcccac
    33001 tgcataaatg acctttacgt aagactcacc cgcacccggt aaagcctctc ccgccgcgaa
    33061 tacgaccggt gaggataaaa gtaccgctgc catcgtggtc ataagcgtta acaacttctt
    33121 tccgaatttc cctaacatct tttcttctcc tttcatgtgt taacttatga tcttttttcc
    33181 cctccttaac acagttcaca tgcctgggga agggaatttt ttcaaactgc gccgggaact
    33241 gcaacagcca tgccatggcg gccgaccccg ccgcccgcgc gccttaagaa tcgacaacca
    33301 tttggtaaat ttgttcctat ccccccctgc ccgctccggg agtaggcctg ccgccgttga
    33361 acatttcatt caatcggcgg cgccgattga atgaaatgtc aaattccggc cccgtcaacc
    33421 gcatggttct tgccggaaac acatttttat tgaaatttta cccggacgca agtaggatcg
    33481 atagaatgca acctgatcct acattaacac agtatcctga aggggtttga taatgcaaga
    33541 aatattgacg gccgttacct atctggtggt gtggttgata tgccagcggg taatcggcat
    33601 tgaaagcgga ctggtcaatc ttatcatctc gctcagcgcc gccgtgggcg tttacgtcct
    33661 gatggcggtc aaggagcaca atgagaagaa aaagagggaa gatcggcatg aataaggccg
    33721 tcgaagaggc gcttgtccag gaacggaagg taatcgtcgg cattatcgag ggctccccca
    33781 tcccgacctt tgtcatcaac cggaagcacg aagtgatctt ctggaaccgg gcctgcgagg
    33841 agctcaccgg cttcgccgcc ggggatatga tcggtacgga caggcattac atgccttttt
    33901 atgggaccaa aaggccgctg attgccgatc tcatcgtcga tcaggacatc gtctccctgg
    33961 agaaactcta cggttccaaa cgggtccgaa agtccgtgaa ggtggaaggg gcctacgagg
    34021 cccgggattt ttacatcgat ctgggtgggc agagccgcca cctctatttc ctcgccgccc
    34081 ccatctacga cgagcagggg gagatcattg ccgccatcga gacccttcag gatgttaccc
    34141 gggaaagggt catggagctc agcctcaagg agcatgcgga gaatatccgg gaggagctgg
    34201 aaaagaacat caccctgcgc aagaccatcg agggtatcat cgagggatcg cccattccca
    34261 tgttcgtaat cggcaaggac cacaagatca tgttctggaa ccgggccttt accgaactga
    34321 gcggttacga gggcaagtat ttcatcggaa cggaccgcca gtacgtcccc ttttatccgg
    34381 aaaaacgtcc ggtaatcgcc gacctcatcg tcaatgacga tatcgagagc ctggaaaagt
    34441 tttacggaaa aaaaaacgtc caaaaatcgc cgattatcga gggtgcttac gaagcctggg
    34501 atttttaccc gaacatcggc ggccggaatc gccacctcta tttccttgct gccccgattc
    34561 gcgacgatag gggggagatc atcgctgccg tcgagaccct gcaggacatc acccgggaag
    34621 aagagatgtc cctgtacctc aaggaatacg ccgaaaccct ggagaacgaa ctgaaggaga
    34681 acatccggct ccgccaggag atcgaggggt tgtacaacta catgcaggca gtcctggaaa
    34741 gctctccgga cacccttttc gaactgaaca gcgacgggat catcaccttt gccagtcgcg
    34801 accggaggca cgacggcatg caggcggatc agatcaaggg gaagcatttt accgagtacg
    34861 ccccggcgca caaagaatat ctcctggaaa aatggcagga aataaaaaag ggcgttttca
    34921 agcccttcga gatggatgtg accacccggg acggcagcag gcagtatctc ctcattaccc
    34981 cctgtcccct caaggggtcg gcggaccggt atgtcatggt ccgccgggag atcaccgaat
    35041 tcaaggagtt ggaaaagaag tattatgaaa gccagaagct ggcggcgatc ggccagctct
    35101 ccgccgggat cgcccatgag gtccgtaatc ccctctcctc cataaagatg agtctgcaaa
    35161 ttctcgaaaa gcgcctgcaa cccacgggca acgacctgaa acgattccgg atcgcccaac
    35221 gggaggtcga acatctggag gaattggtca acgatgtcct gatttttgcc aagcccgggg
    35281 agccgaagcg catgcccacg gatctgaggc gcgccataga aaacgccctg gagatggtgg
    35341 aaaaggccgt taccgacaaa catatcgccg tcgtgatcac gatggcggag gagctttccc
    35401 atccaaacat cgacggcgcc atgatggagc aggtcttcca gaacatcttc cggaacgccg
    35461 tggatgccat ggaggagggc gggcaactgc ggatctccgc tcgggataac ggtggcgcga
    35521 cctccgccgt tgtcatcgag attgccgaca acggctgtgg catggacgag gaggttcttt
    35581 cccacctctt caatcccttt ttcacgcaga aacgatatgg aacgggactg ggcatgaccc
    35641 aggtgaaaaa gatcatcgac cttcacaaag gcaacatcga tgtcgaaagc agaaaaggcg
    35701 aggggacgaa tatagtgatt accctgccta tgaacatctg agtaatctca atatcaggag
    35761 cccctcatgg caaagattct ggtcattgac gacgacagtt ccatcgcgga aagcctcgac
    35821 ctctacctta cagaggaagg atatacggtt tacactgccg ccacgggaac cgacggtctc
    35881 aatgccttcg tgaagcacgc tcccgacctg gtcgtactcg atatccgtct ccccgacatc
    35941 gacggtttta ccgtcttgga agatctgcgg gaggaggatg agaacgtgaa ggtgatcatg
    36001 atcactgcct atcacgatat ggattccacc atcaaggcca tgaagggagg ggccttcgac
    36061 tatatccaca agcccataaa cgtggaagaa ctggacatgg ccatcaggaa ggcccttaag
    36121 accctggaga tggaaaagaa gatcagcggt ctgctcaacg agccgtcccg gagtttcaag
    36181 gttggcgaca tcatcggcaa tacgaaggaa atgaaggaga tcttcaagac catcggcgtc
    36241 gtttcccaga gccgaaccac ggtcctcgtc gagggagaaa gcggcaccgg gaaggagctg
    36301 atcgctaaag tcatccacaa caatacctct cccgatgagc cttatatcgc cgtcaactgc
    36361 tccgccattg tggaaaccct cctggagtcg gagctcttcg gacacgaaaa gggatctttc
    36421 accggcgcca ttacccgcaa gctggggaaa ttcgaactgg cccgttacgg caccgttttt
    36481 ctcgacgaga taagcgagat gtccgtgaac ctccaggcaa aacttctgcg ggtcctccag
    36541 gagatggatt tcgagcgggt aggcggcaag gattccatca aggtcaacgc ccggatcgtc
    36601 acggcgacca acaaggatat caaatcgatg gtcaaggagg ggaagttccg ggacgatctc
    36661 tactaccggc tgaatatcgt ggcgatccgc ctcccccccc tccgcgagcg gcgggaagac
    36721 atcccggccc tggtggatta ccttctgacc aagatcaatc gggaactcca caaaagggtc
    36781 atcggcatct ccgatgaaat gatggacctg ttcatgacct accgctggcc aggcaacgtc
    36841 cgggaactgg aaaatctcct gacccgggcc gtggtggtga ccaaaagcca ggtgctgacc
    36901 cggaacgatt tccccgatct ctccgaagag gtcgtggccg ccgacgatgg caaagggaaa
    36961 gacgccgtcg aaatcaatgc cggcggactc ctcaccctgg aggcggtgga agagcagtat
    37021 atcagaaagg tgatcaagga gaacagtcac cgcaccaagg gagagttgtg cgaaatcctg
    37081 ggcatctccc ggccgacttt cgagcgcaag ttggaaaagt acggtcttgc catcgagcgg
    37141 gattgagatc gctccatgaa gatcaggtcc gcggagtttg tcaagacggc gctccttccc
    37201 tcccattatc cgccgcccca atttccggaa atcgcctttg ccggtcgttc caacgtgggg
    37261 aagtccaccc tgatcaacac cctggcgaac cgggtgggtc tggccagggc cagccggacg
    37321 cccgggcgga cccaggccat aaatttcttt ttggtaaacg aaagattctg ttttgtcgat
    37381 ctgcctggct acggtttcgc ccgggttccc gaccaggtcc ggcgggactg gggtcctatg
    37441 gtggaggcct atctggcggg gagagaaaat ctgaccatca tcatcctgat cctggatatc
    37501 cgccgggacc ttcgggagga cgaccggaat ttcctgctgt ggatggagga gaagggacgc
    37561 ccctggcagc ttgtcctgac caagacggac aaactttcgc accagcaggc cctgacgcgc
    37621 cggcgccaca tcgaggccca ggccggtatc cccgtacccc gggaacccat tctcttttcc
    37681 gcccgcacgg gccagggaag ggggccgctg tggcggatca tcgaagcggc ggcggagacc
    37741 ccttgaaccg cccagggccg gcggcaaagg aaacatcgcc caccggagga accgtggtcc
    37801 ggaatgatcc aaatatctcc ggaactttaa aggagttaac ctcttggacg gaaactttta
    37861 cccttgacag ggatgctgaa tcatgatagc ggcaccgtcg gttgaagtcc gcaaaatccg
    37921 gtcagggaaa aaggtcgtcc ttgaagactt ataaaatata taaacccgca tatacccatc
    37981 ttttgaggtg tctctcgtga tcaatgacaa tgaaaaaatc ggttcggtta tggttgtcgg
    38041 ttccggcatc gccgggattc aggcttccct cgatctggcc aattccggct tgaaggtgta
    38101 tctggtggaa aaggacatca gcatcggcgg cgtcatggcc cagctcgaca agaccttccc
    38161 caccaacgac tgttccgcct gcatcctctc ccctaaactg gtggaggtgg gcaggcatcc
    38221 caacgtggag atccttaccc ggcgcacggt caatgccgtg gaaggggagg cgggaaaatt
    38281 cacggtccgg atgaccaagg ccccccggtt catcgatctg gacaagtgta cgggctgcgg
    38341 agactgtgcc aaggtctgcc ccatcaacgt cccttcggac ttcaacgagg gcctctcaga
    38401 gcgccaggcg acctatcgca agttccccca ggcgattccc agcggcttcg ccatcgacaa
    38461 actcggcacc tccccctgca aagcggcctg tcccacccac atcagcgttc agggatacgt
    38521 ggccctcatc gccgcgggta agtacaagga ggcactggcg ctcatcaaga aagacaatcc
    38581 cttccccatc gtctgcggcc gggtgtgcaa tcatccctgc gagtcggcct gcatgcgcgg
    38641 caaggtggaa gagcccatcg acatcatgca cctgaagcgt tttgtcgccg atctggatct
    38701 gaagggcgag acccgcttta tccccgagaa gaaggagagc aagggcaaga aggtggccgt
    38761 ggtcggcgcc ggtcccgcgg gtttgacctg cgcctactat ctggcggcgg aaggatatga
    38821 cgtggaggtc ttcgaatccc tccccgtggc gggcggctgg ctcgccgtgg gcattcccga
    38881 gtaccgcctc cccaaggacg tgctccgggc agagatcaag gtcatcgagg atctgggggt
    38941 caagatccat ctcaacaccg ccgtgggcaa ggacatcccc ttcgggaagc tccagagtga
    39001 ctatgacgcg gtgttcatcg gttgcgggac cgtcaacagc agcaagctca acatccccgg
    39061 cgaggatatg cagggcgtca tccacggcgt cgattacctc aagcgggtca atctgggcga
    39121 aaaggtcttc ctgggcgaca aggtggccgt ggtcggcggc ggcaacgtcg ccatggacgc
    39181 cgtccggacg gcagtccgca cgggatcgaa agacgtcttc attctctacc gacggtcccg
    39241 ggccgagatg cccgcctcac ccgaggagat cgaagaggcc ctggaagagg gcatcaagat
    39301 ggaattcctc gtggccccca agcgggtggt cggtgaaagc ggcaaggtga ccggggtgga
    39361 atgcacccgc atggaactgg gcgagcccga tgcgagcggc cgccgccggc ccgtggagat
    39421 caagggatcg gagttcatcg ttccctgtga cgccctgatc cccgccatcg gccaggaggc
    39481 ggatctcgat ttcgttcccc aggagagcgg catcgccatc aataaatgga agaatttcga
    39541 tgtcgatccc gtgactttcg ctgctaatgt ccccggcgta ttcgctggcg gcgacgtggt
    39601 cacgggtccc cagacggtgg tcaaggccgt ctatgcgggc aaggaagccg cggtttccat
    39661 cgaccgttac ctgaagggcg aagacgtggc ggcgggccgg gcgaaagact ggaaacaggg
    39721 tctggccgac aaggccgacg tctccaaggt agccaagatt gcccggatga agtatcccca
    39781 tatgaaaccg gaggaccggc ggacgaactt tagcgaagtg ggcatcggct tcacagaaga
    39841 agaagccgta gccgaagcta agcgctgtct ggcctgcggg atctgctcgg aatgctacca
    39901 gtgcgtcgat gcctgcatcg ctggtgccat taaccacgac gacacctttg aagaagaaac
    39961 catcgaggtg ggagccatca tcgccgctcc gggttttgaa gtcttcgatg ccaaacaccg
    40021 gggtgaatac ggttatggga tttacccgaa cgttgtcacc agcatccagt tcgaacggat
    40081 cctctccgcc tccggcccct atttcggcca tgtacagcgg atcagcgatg gtcgggaacc
    40141 gcagaaaatt gccttcatcc agtgtgtggg ctcccgggac acctcctgcg gaaatagttg
    40201 gtgctcctcg gtttgttgta tgtacgccac caaggaggcc atcatcggta aggaacacgc
    40261 caaggggctg gaaccgacga tttttttcat ggacatccgg gcccatggca aggacttcga
    40321 ccggttcgtc aaccgggcaa aagacgaata cggcatccgt tacatccgtt ccatgccctc
    40381 gacgatcaag gaacttcagc aatccaacaa cctgcttttg aagtatgtcc aggaggacgg
    40441 cagcctcgtc gaggaggaat tcgacatggt ggtcctttcc gtgggtcttt gcccgccttc
    40501 cgaggcggcg gaactggcga aggccctggg catcgagctg gaag
//
LOCUS       DAOSMF010000007        38805 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_1399_length_38805_cov_23.184981, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000007 DAOSMF010000000
VERSION     DAOSMF010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 38805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38805
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1399_length_38805_cov_23.184981"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="PLT63_02065"
     CDS             <1..458
                     /locus_tag="PLT63_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97533.1"
                     /translation="DQAGKNALRALNIPCVQVGYLDLKGNNGNLEVNMLGVKFFAHSS
                     GATPSLFATNNVNGSYTTTAPTAGTSVPLSATAGGTIGTSTFTVQSWNSANNGKWSAT
                     VQGANANIGSTANLQGLNIKGAAAGNITGTNFSGTAAGVAKQGALPTPE"
     gene            743..3376
                     /locus_tag="PLT63_02070"
     CDS             743..3376
                     /locus_tag="PLT63_02070"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HOD97534.1"
                     /translation="MNDRISRDDLLHYVVKPSRYLGGEVNAVRKDHRTCRLSVALAFP
                     DTYEVGMSHLGLQILYELLNSRDGIIAERVYAPWPDMEELLRRHAIPLATLESGTTLR
                     DFDLVGFSLQYELSFTNVLTMLELGGIPRRRTDRRDGDPLVIAGGPCCFNPAPMTAFI
                     DAFVIGEGEEVSGEMATVMMREKDRGSGREKLLAALAELPGIYVPAVHTRGEKIRKRV
                     VAELNTWTLPVRPLTPLMKTIHDRAVLEIARGCTRGCRFCQAGMVWRPVRERQPGILQ
                     EMADQMLAATGQEELSLLSLSSGDYTCIETLLPLLMDRYYARRIALALPSLRVETLSR
                     NLIEAIRRVRKTSFTLAPEAGTQRLRNVINKGNTETDLLATSAQVFAAGWRAVKLYFM
                     IGLPGEEEGDLEGIATLAHQVLRTGKKRGQVTVSLSNFVPKPHTPFQWQRQIGPEETA
                     LRQEFFKSRLRHRQIQIKWHDARMSRLEGLFSRGDETLGDLVELAHRGGCRFDGWSDR
                     FRPEIWDDAIRALGIDIAANLAARSRNAVLPWDHIDAGVERDFLWEEGQKARGEELTP
                     DCRREGCYRCGACDHIEIRPLLAEDGPPENHSATATGGPEGMTTESLVDLDVAEATAT
                     SPVTANNAAETGAFGIHLAGPEQPAREAPGQRNGQRLRLRFTKQGRSRHLSHLETASA
                     LTRGIVRSGRRFLFTAGFHPHPRMSFAVATAVGLASEGEYCDVVWEKTTDETASLPEE
                     INRFLPEGMLITELVELAPDAPSLTTSLRGFQYTAALPPRLNAAALDALEGKIAAFLA
                     AAAFPVARTVKDHTIIRDIRPLVTNLIMDRTGRRMLMEVRFTNEGAVRPEEILVHALG
                     LDPSLPAVMNLTKTATFFIDK"
     gene            3429..3740
                     /gene="rplU"
                     /locus_tag="PLT63_02075"
     CDS             3429..3740
                     /gene="rplU"
                     /locus_tag="PLT63_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041584674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOD97535.1"
                     /translation="MYAVIKTGGKQHRVSVGDMVAIEKIDGAKGDTVVFDNILMVAAE
                     GQIRIGTPAVNGATVTGEIVAQTKAPKIAVFHMKRRKGHRKKTGHRQPLTRMRIRDIS
                     F"
     gene            3758..4012
                     /gene="rpmA"
                     /locus_tag="PLT63_02080"
     CDS             3758..4012
                     /gene="rpmA"
                     /locus_tag="PLT63_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002778821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOD97536.1"
                     /translation="MAHKKGQGSSRNGRDSNSQRRGVKVFGGQAINAGGIIIRQVGTK
                     IHPGRNVGLGRDYTIYAKIDGVVKFERLDKTRKKVSVYAA"
     gene            4088..5131
                     /gene="obgE"
                     /locus_tag="PLT63_02085"
     CDS             4088..5131
                     /gene="obgE"
                     /locus_tag="PLT63_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOD97537.1"
                     /translation="MHQAPLFLLPMKFVDEVKIFVQAGHGGRGCVSFRREKYVPRGGP
                     DGGDGGKGGDIVFQADRHLSTLLDFRYRPHHLAPKGGNGEGSNRTGRNGEDIVISVPV
                     GTVVKNAATMEIMADLVEDGQRFIAARGGIGGKGNSHFATSTHRTPRFAQDGMPGEEF
                     WIILELKLLADVGIIGKPNAGKSTFISRVSAARPKIAAYPFTTLTPNLGVVAYGRHQS
                     FVIADIPGLIAGAHAGAGMGVQFLKHVERTSILLHIIDISEDNSPRASRLHDEVNREL
                     GLFNAGLLAKPQVVAIGKIDLPAVRERLKKEIDIFREKGIEVSAFSAVTGEGMETVMN
                     RIALAMERASATC"
     gene            5321..6460
                     /gene="proB"
                     /locus_tag="PLT63_02090"
     CDS             5321..6460
                     /gene="proB"
                     /locus_tag="PLT63_02090"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HOD97538.1"
                     /translation="MTRKEILKDVKKMLIKIGSAVLTGDNGLDLHIIEQLVEEMVELK
                     KRGCQIVIVTSGAIASGKHRMGLTGHLKSMPQKQAAAAIGQGRLMRVYSNAFGKYGLY
                     VAQILLTMSDLTDRKRFLNIRNTLTTLMDWGVIPIINENDTVAVDEIKFGDNDHLAAM
                     MANITETHLLINLTNTEGLYDHNPNRSKKAKVIPLVREISEVIEQAATDETSDVGSGG
                     MRSKVMAAKKVTAFGIPYIIAPGKEPGILRDLFAGKERGTLFLPMKDHLNSRKYWIAF
                     TLRSRGKLTLDDGAKKALIEEGKSLLPSGIIAVEGDFHVGDPVLCLDTAGKVLAKGLT
                     NYGAEEIGKIMGLKTSKIQQVLGYKDYDEVIHRDNMAVDKELQHK"
     gene            complement(6630..7184)
                     /locus_tag="PLT63_02095"
     CDS             complement(6630..7184)
                     /locus_tag="PLT63_02095"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HOD97539.1"
                     /translation="MVASWKYESVPSPWGEVAVVWRVGGEEGGVKVAAIILPSEKNGP
                     EGVWKRRFPGIVRENSPAIRLFCADLQRALKGQAVRFSLDLLDMAFCTDFEKKVLAAC
                     FRIPQGAVISYGALAERVGSPRGARAVGRVMAGNPFPIVIPCHRVVRGDGTLGGYGGG
                     LAMKKVLLAWEGIQVPAPRGRSQG"
     gene            complement(7272..7835)
                     /locus_tag="PLT63_02100"
     CDS             complement(7272..7835)
                     /locus_tag="PLT63_02100"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HOD97540.1"
                     /translation="MTNLWPILLIAVALGMDAFSVAVGIGATRHRVSLTSILRLSASF
                     GIFQFFMPIVGWLGGVTIAAYIDSYDHWIAFAVLLYVGGKMIHESFRGEEDPAPGQDP
                     TRGVTLIMLSVATSIDALAVGLSLAVMKTPILYPSAVIGIVAFVMTAVGMLFGERLGK
                     MFGRRVEALGGLILIGIGLKILWDGLA"
     gene            complement(8017..10080)
                     /gene="uvrC"
                     /locus_tag="PLT63_02105"
     CDS             complement(8017..10080)
                     /gene="uvrC"
                     /locus_tag="PLT63_02105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOD97541.1"
                     /translation="MEPAAMTGTCLADIVQVAPRVPGVYLMRDGVGEIIYVGKAKDLR
                     ARLRNYAGGTDSRGMIPFLLSRVRQVEWIITATEKEALILENNLIKEHRPRYNVVFRD
                     DKAYFHIRMDTAVPFPRWQLVRRPKKDGARYFGPYPSGGAAKETLRFLQTVFPLRTCR
                     DVELRNRRRPCLEYQIGRCPAPCVHLVAEETYRRSVRQGLAFLEGNATAVLKELEERM
                     ENAAAELRFEEAAALRDRIGAIRTTLEKQQAALIAAQDQDVFGAAREGDWTQLCLLRI
                     RAGKMVGMRTFPPLRVSLPMKEIMTTVVMQFYDTGNEIPGEILLPAAGEDRRVLSEWL
                     GEKRGRRVRLFVPQRGRGRALVEMAGRNATEIMQVVRRHQGTGGDLEALAAALELRRP
                     PERIECFDISNIGGRYSVGSMVTFAGGVPRRSDYRRFRIRTVPGADDYAMMYEVLSRR
                     FATGKDLPDLLVVDGGKGQLAVALSVLKDLRIGSVDVIALAKERDFPPGSRGRGLLGR
                     EGQTGATGGAGETSSLGTERGRRHSFRSDVKHPDGAADQETGGMEMVRSETMGKETVG
                     AEDENVKLGRGRISDRIFLPGRKEPVFLSRYPAVLHLLQRLRDEAHRFAVTYHRRVRE
                     QEDFASRLDDIPGLGPEKKKALLRGMGDLERIRTATAEELRVIPGIGKKLASRIEDFL
                     RPIKI"
     gene            complement(10220..12226)
                     /gene="uvrB"
                     /locus_tag="PLT63_02110"
     CDS             complement(10220..12226)
                     /gene="uvrB"
                     /locus_tag="PLT63_02110"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF003673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOD97542.1"
                     /translation="MKAFELVSEYSPRGDQPRAISRLVQGVKADLPHQVLLGVTGSGK
                     TFTIAHVIQSVQRPALVIAHNKTLAAQLYAEFKSLFPANAVEYFVSYYDYYQPEAYIP
                     STDTYIEKDSAINEEIDKLRHAATHALFDREDVIIVASVSCIYGLGSPEAYQGMLLYL
                     EEGRELGRDTMLRRLVEIQYERNDFDFHRGTFRVRGDVVEIFPAYEDERAVRVAFFDD
                     TIEEILYVDPLRGRKLGRISKLAIYPGSHYVTSRDNLNRAMTTIRAELQERLSELRRE
                     EKLLEAQRLQQRTLFDLEMMEEMGYCQGIENYSRHLTGRNPGQPPPTLMEYLPKNALI
                     IIDESHATIPQLIGMYRGDRSRKETLVEYGFRLPSALDNRPLRFEEYEAFPQRRIYVS
                     ATPAAYELMKASGGVVEQIIRPTGLMDPEIVVKPVKSQVDDLLGEIRQRAARGERVLV
                     TTLTKRMAENLTTHYGEVGVKVRYLHSDVHTLERVEIIRDLRRGEFDVLIGVNLLREG
                     LDIPEVSLVAILDADKEGFLRSERSLIQTAGRAARHIAGQVIMYADVITGSIRACLDE
                     TQRRRLLQTDYNRRYGITPESIRKSISDVLTSVYDADYVTVPLVRETKVPYGGEEDLT
                     KLIARLKAEMKAAAAALEFEKAAELRDEIRDLSSLLVEFGGESV"
     gene            12464..14197
                     /locus_tag="PLT63_02115"
     CDS             12464..14197
                     /locus_tag="PLT63_02115"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOD97543.1"
                     /translation="MEATKESAHASVDSFIHARKAKFTMNLSPVTQLLAFLDWFINLA
                     DSPGKQYRIMEDFWEKTANYWDYVLRSCLLSNGEPEKPIARDPRFRDDAWQTPPFNCF
                     HQAFLLSEQWWQNTTTGIPGVSHHHQNIVSFMARQIMDVLSPANFLWTNPVLTRVTEE
                     QGGQNLIRGWENMLEDWRRQRNNELPAGTENFRPGEKVAVTPGKVVFRNHLIELIQYT
                     PTTKKVYEEPVLIVPAWIMKYYILDLSPHNSLVKFLVDKGHTVFIISWLNPTEKDRNL
                     SMADYHFDGVMAAINAIAKIVPDRQINTAGYCLGGTMLAIVAATMARDGDERLKSMTL
                     LAAQTDFTEAGELSLFIDENQVNFLADIMFDRGYLDTKEMAGAFQLLRANDLLYSRII
                     QDYLLGARRPLNDLMSWNADATRMPYRMHSKYLRRLFLDNDLFEGRYLVNGRNIVISD
                     IRVPVFLVGTVQDHVAPWRSVYKFHLTSDAPTVTFVLTSGGHNAGIVSEPENTRRTYQ
                     MLTHHEGDKYIDPDRWLEEAPKFKGSWWLPWQEWLAANSSGMTVPPGMGAPDKGLKIL
                     GDAPGTYVRQI"
     gene            14291..14428
                     /gene="rpmH"
                     /locus_tag="PLT63_02120"
     CDS             14291..14428
                     /gene="rpmH"
                     /locus_tag="PLT63_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOD97544.1"
                     /translation="MKKTLKPVSNTRRKRTHGFLVRMATKGGRLVLNRRRAKGRKRLA
                     V"
     gene            14481..14828
                     /gene="rnpA"
                     /locus_tag="PLT63_02125"
     CDS             14481..14828
                     /gene="rnpA"
                     /locus_tag="PLT63_02125"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOD97545.1"
                     /translation="MERSTFGKQERIRKRRDYLTVYQQGIRDHSGHFTCLTCRNLSGK
                     RRLGITVGKKVGKAVKRNRIKRIIREFYRVHKQRLPASLDIVIMAKPQVNMLTNHEIR
                     RALERLFDRIKDE"
     gene            14821..15057
                     /gene="yidD"
                     /locus_tag="PLT63_02130"
     CDS             14821..15057
                     /gene="yidD"
                     /locus_tag="PLT63_02130"
                     /inference="COORDINATES: protein motif:HMM:NF013932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOD97546.1"
                     /translation="MNNKVLSQTLAKILVLVIRLYQISISPVLPPSCRFFPTCSEYAI
                     QSIRRYGPLRGTIAGLKRLGRCHPWHPGGYDPVR"
     gene            15079..16704
                     /gene="yidC"
                     /locus_tag="PLT63_02135"
     CDS             15079..16704
                     /gene="yidC"
                     /locus_tag="PLT63_02135"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOD97547.1"
                     /translation="MDKRTILAIVLSVTVILIYQMFFMKPPVKEPAPPQETAQTEKKD
                     VPASAPPPTAPSAPTQIKAGKGIVPPAPVVAERFITVDTPLYSAQFTTKGGALHSFKL
                     KGYRQKLVASSEPIEMVDVAPGMPRPLTVSFPGSSLDPPGEGMYESETKSLALTDTKE
                     PQRLVLRHTVANVMKIEKIFTFYPDRYAIDLEVRTFNLNAVPLSQNASINWNQYADPK
                     EETDSWSHKGPVAFVAKDIERIDVKKIEGGKLLGPNVTWGGFESKYFIAAVIPQNPTL
                     TSLAVAKDGRDMVSVSLQGPKILVPGGQADSFIYTLFIGPKDYSILKVQNVHLENAID
                     FGSWLKWLAMPLLILLKWLYQYVHNYGIAIIILTILIKLIFWPLGNKSYESMKKMQKL
                     QPKMKELQEKYKGDRQKLSQETMALYRTHKINPMSGCLPMLIQIPVFFGLYKTLLYAI
                     ELRHSPFFWWIQDLSEKDPYYITPIIMGGTMWLQQKMTPVGGDPMQQKVMLWMPVIFT
                     FLFLSFPSGLVIYWLFNNIISIGQQYYINKRHS"
     gene            16717..17580
                     /gene="jag"
                     /locus_tag="PLT63_02140"
     CDS             16717..17580
                     /gene="jag"
                     /locus_tag="PLT63_02140"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HOD97548.1"
                     /translation="MKMIEFEGKTIDEAIEKACRELGAPREKLNVEIVAEGTSGFFGL
                     GSKRAKIKARLMDINIDEEHVVDPIAVTARSKAPETKDAPSHPPTRAAAEAAGANLAA
                     KEDKPLRKYAPPGASSPTDQEVGARAQAILSGILERMNIASPVAVDIREDAIVLNIKG
                     DSGGLLIGKRGQNLDALQYLVNKAANRSGEERKMIIIDTEAYRQRREDALASLAERLG
                     QKVKKTRKAVTVSHMNAHDRRIIHLTLQGDSSLVTKSRGEGEYRKIIIMPARKERERD
                     RGETTTTTGVR"
     gene            17588..18970
                     /gene="mnmE"
                     /locus_tag="PLT63_02145"
     CDS             17588..18970
                     /gene="mnmE"
                     /locus_tag="PLT63_02145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOD97549.1"
                     /translation="MQADDTITAVATPPGVGGIAVIRLSGPAAEQIGKTIFRGAHNHP
                     PKFTTHRLYHGHIVSAVDSAVIDEVLAVLMRAPHSYTGEDVLEIHGHGGYLLPQRILA
                     ETIRAGARPAAPGEFTKRAFLNNRLDLSQAEAVADMINARTERGLELARSQYQRQLTA
                     EITALREGIVDAIARLEAAIEFAADDPDIADDMSIPNQLQEIGHEVNSLITTYREGKI
                     YRHGVSLVIAGRTNVGKSSLLNHLLGEKRAIVAASPGTTRDFIEETVNIHDIPVRLTD
                     TAGIRSTADAVEQEGIAMVWQRIDAADAVLLVFDGSENLTTADRQIVAKLAGKALIPL
                     VNKVDLPRMLAPEVLSDLLPNHQAIWISAKHGTGIDELLQAIHHLFLATERETSAGTV
                     ITSLRHKLALEKASFFICQAMARAARGGAPELAATDLQDALEALEEITGRTADETILE
                     RIFANFCVGK"
     gene            19162..20193
                     /locus_tag="PLT63_02150"
     CDS             19162..20193
                     /locus_tag="PLT63_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97550.1"
                     /translation="MNNDRPSARMEAVARAKWHAIEQALGVDVKAGSYVSDFALVEDV
                     MKTQVGGSVKKFRILSQTEKDSVLTARINACVEPKKAQEAIASELALNSGIAVFIPAR
                     KPQGTTGEDVFEETNILSENLIGRLTEQHYRVVDVAPTHALAAAEIEKAMRSGSTLAV
                     RSMLYKFLSNIAVIGRTDYTVSTKKGEDIGYGLYMPFNNVTVRLTYRMVVRNKKTGNM
                     EILSAGTEQGKGIRNSVEDAAAVALKDVADKVIPKILDKAAQYLQGNVKRIRVKIAGV
                     KDIETNIDIKGILQNIVWVSEVEELKMGEFIVAYPENSVYLANSIKQKGNFTVDNFTP
                     YSLTLSYRP"
     gene            20204..21310
                     /locus_tag="PLT63_02155"
     CDS             20204..21310
                     /locus_tag="PLT63_02155"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HOD97551.1"
                     /translation="MKRAGLSGLLLFLILSGCASAPKLDLTTFVTDREAVIRVPDICK
                     PRYDAALPYVAVVNFTNNTTFDYVRIMQSRIQDSGFQADAQSISREVNAKLGESIEAG
                     VTDEIVNTGGAKVITRSEMNKVFEEQQFQQSGLVDDATMIDIGKIKGVKYIVTGSINN
                     VDMRWISHPSTRQGLGKGGSGSWAFDLIGVAIAASLEAQEGWHIGAEVTLRILDVESG
                     EVLLSKKMNGRHNIGRIPYPNYDVLIGGIKKAAGKSLQAARPELSKYFPLKGYITMIK
                     NSPEGKKKAVRINIGESEGIRPGDELFVYTFEEVDDPMSGGKECDIQRLPVTLVVSRN
                     QLRPDAAWALVKAKTPEQLRTIRVGQLVERKTQR"
     gene            complement(21431..22183)
                     /locus_tag="PLT63_02160"
     CDS             complement(21431..22183)
                     /locus_tag="PLT63_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HOD97552.1"
                     /translation="MKVIYSKEFDISYCSDPAAAFGRIEAVYSVIKDRVKIINAVPAA
                     EEDIAAVHTQNHLEMVRREGIYDIAALAAGGAVQAAQIGMKEPCFALIRPPGHHASAG
                     SSWGFCYFNNMAIALEKLKRDGLINTAMVLDFDLHFGDGTVNILGSKGYVNIYNPSSS
                     SRRDYLQGVRSFLEGGRADIIGISAGFDNHVDDWGGLLQTGDYREMGELVREAAARNK
                     GGYFAILEGGYNHRVLGQNVLALIEGMESAGA"
     gene            complement(22188..22376)
                     /locus_tag="PLT63_02165"
     CDS             complement(22188..22376)
                     /locus_tag="PLT63_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97553.1"
                     /translation="MYKRSMTVYREGIDRRNNRLTPPEQFRVMERDEDGVFTGVNIRD
                     ISEAEMRAARSRPYNVFR"
     gene            complement(22637..23065)
                     /locus_tag="PLT63_02170"
     CDS             complement(22637..23065)
                     /locus_tag="PLT63_02170"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOD97554.1"
                     /translation="MTRREYPQRPIVGIGAIVLKDGKVLLIQRAAPPSQGLWAIPGGS
                     LEAGETIQEGAEREILEETGIRIKAGNPVYAFDFIQYDDRKGLQYHFVIVDVEAEYLG
                     GEVQAADDALAARWVSPAELPDLPVSANTLKILRSRGFLT"
     gene            complement(23077..23841)
                     /locus_tag="PLT63_02175"
     CDS             complement(23077..23841)
                     /locus_tag="PLT63_02175"
                     /inference="COORDINATES: protein motif:HMM:NF017551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HOD97555.1"
                     /translation="MCLIIFAWRQRADYPLVVAANRDEFYDRPTLAADFWDDHPEILG
                     GRDLKEKGTWLGITRKGHFAALTNYRDPRHVNVDAPSRGWLVRDFLTGEKGAAEYLGD
                     LQKEGGRYNGFSLVLGDEDDLYYYSNRGSAPFLLPPGIYGLSNNLLDSSWPKVIKAKE
                     EFRRVMTRAGRLHTEDLFALLADRSQPGDEELPDTGVGLEWERILATIFIASPIYGTR
                     SSTVVFKDRQNRIHFEERQYAGGDGLWMSTGFVLEN"
     gene            complement(23832..24623)
                     /locus_tag="PLT63_02180"
     CDS             complement(23832..24623)
                     /locus_tag="PLT63_02180"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HOD97556.1"
                     /translation="MSDIDLETMINEAVAMIAAAGKIVVFTGAGVSTESGIPDFRSPG
                     GLWSRFDPDDFTIERFLSSPETRKKQWKFLFEGGIFAQARPNDAHRAIAWFEEQGKLV
                     CVITQNIDDLHQQAGNSPERVYELHGNMKWMKCLACLERYPLPVMLARYRESGEIPGC
                     ERCGGLLKPDVIFFGEALPPQTLKEASDAASNCDLLIVIGSSLVVYPAAYIPLYAKGG
                     GAKLMIINRTETSYDANADLVIRGGAGEVMTGIVANLKRTGGLCA"
     gene            24844..26310
                     /locus_tag="PLT63_02185"
     CDS             24844..26310
                     /locus_tag="PLT63_02185"
                     /inference="COORDINATES: protein motif:HMM:NF014510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HOD97557.1"
                     /translation="MQPLLTFVNNLRIQDVLDVIIISLMIYFLLVWFKNRASRFVLIG
                     LSVVGAVYVTARFFQLYLTTVVLQGFFAILIFVLVVIFQEDLRRLFERLAIIGRFGGQ
                     STASTKRGDTEILAATATNLARNHIGAIIVLKGKDPLERHLGSGTALDGLISQELLES
                     LFDPHSPGHDGAVIITGNRITHFGCHLPLSTNMGRNGATGLRHTAALGLAERADALCI
                     VVSEERGSISLARNEKIRVLSSGLELRNELEKFYRELAPEKRTASRISLELRKNTREK
                     IIAVALACILWVTFGYQKETVRREYLVPIEHLDFSRHFVLEEPQTREVKVILSGTTQA
                     FQLLDSGSLKISLKKAGIREGRQSIVLTSDMVRTPSNINVAAIIPPEITVTATRYLPV
                     SIPLEVTTTGKPPRGIAVQKISVDPAAVRVFIPGVAVPGKIKIQTEPVDLARLSEQTS
                     FDVTLRYPPNVKFPDDKVPSTVVTVKIRKKAGGGDQDR"
     gene            complement(26560..30489)
                     /locus_tag="PLT63_02190"
     CDS             complement(26560..30489)
                     /locus_tag="PLT63_02190"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HOD97558.1"
                     /translation="MLSLHAGKRFLAVFLVAAVILTILGATAWLFGSENGSRWLVGRL
                     LGMIPARVETGQISGALGTELSVADLQVGGEGWRLMVRRTVVQWRPWELWAGKVFLGK
                     LMLDGVDFQDDRPATPLDLTWPRLPDFLSWFRGGIDVLDVRNILWRSADREITIPHMA
                     AALDYTYGLASLSHLAASVPGGKLSGRLQLHLTSPFLETQLKFAPDDGRWLETTLRAS
                     LRASADSLHLQGPVSVVIVDGKRERFILKGQAATSAHILALTGLSGEEQNRAGALKGE
                     ARLDFSRPAVRLSSTLKIDRLDLSPEVGRELVLGGDLELAGNLSSYEGRFHLRPRLVA
                     GPPLAVRGSFSGAGERIAIDGLQINVGQGIARGRATVAWERGWTVSWALRARNMDPSL
                     AVSQARGVINADLDGIWRRGAGGETSGKLAGRFLDSRLSGRQMTGGVGMRWEGDALFL
                     EKLEVRGEGFQAQARGDLRDRIGFEVRIARISRLIPQADGRLYGRGWVRYRNRLLSGA
                     LTAAGKKISWDQDRIDDLALSLSLGEGEAGELRAWISAKDLYVAGQCLQGEFTVTGTR
                     KSQELNLRLTGPEGGLAVRADGGYERGVWTGAVGTAAADSKSYGVLQLSRPASLSVSP
                     HLLRIENFIMAGPQGERLEISALLGDKKQPGYATASWQNFPLGRLPLPTADMHLNGKT
                     TGQVRIEQRGVREGFQAAGTARLQGRIVRGEHRLEVQDGQLRFQWQAKGLEVSSSFDL
                     GLMGRLNVRLSSSSAPFFSFPDSGSYEASLADLDLQLLNPFLPSSLRTEGILSGRLEG
                     RLLPQRRFAMTGQAEAVKGIWTVAGRRGMVRFVQERLALGWTWRDDILRGTMEGAFTD
                     HGHLRAVFSLPVRSQYPVGINPQGPISASVKGEIREKGILGAVFPGTVRETHGRLAVD
                     LAVAGVWQNPRYEGHLRLLKAGAYVTATGIRLRDMEAEARFFPERIEIEGFSARSGPG
                     NLKGRATIRYGGGNIKDIKGHLEGERFQASYLPELQLLVSPRITFEGTREYLSVRGAI
                     HVPEGRYLEPREPDLIRASKDVKIIDKPVKKAPAVPFVVDMDLAISLGEQVYMRTRDI
                     EGNFSGEMTASGKKLEDLPARGEMHITHGYLHAANTKLPIERGHIFFKDKPFSLATLD
                     ILAVKEVGDVRAGFLVTGTLRAPVATLYSVPSLPDQDVLAYIVFGTSYTGDKIQATTL
                     LKSAGMFLAQGKSGGLEDSLRKSAGLEVGGITSPSRSKQGRTDMTTSLSTMGQYLSPQ
                     LYVGLARAMFSDDILYVMKYSFSRLWEVETKAGRQSSIDLFYKLEFD"
     gene            complement(30546..32297)
                     /locus_tag="PLT63_02195"
     CDS             complement(30546..32297)
                     /locus_tag="PLT63_02195"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex family protein"
                     /protein_id="HOD97559.1"
                     /translation="MDPYKCLIPFLIVLLAASLTWAAAPVEVLVQGLEGKELKNVQTA
                     LVVPPGLVKKDRIDEAWLDHFLDQIPRKVQEALEPFGYYHGTATVSQEAAPEGHMKVQ
                     VTVTRGDPVLVRGIAVRIEGTGRDEEDFRPLVANFPLAPGMVLRHEVYEEAKRGIHDV
                     AIRRGYLDGHFLVQEVRVNLGERTADIEIVYDTGSRYFFGAISFSGAPLYPRPFLNRY
                     LAFQEGEVFSYEKIGETQRNLTLADRFADVRIETDKEKARDHRVPVEIKLRPGPEKHL
                     KLGAGYGTDTGPRASINYRDKNIIDAGHYWEGGINVSRVFQGLALGYTVPGRDVRTFS
                     SLGVNLKRDNPDTYDTKEFSMELQHVRPYFLDKTGSVFLQLLREDSTAGDAQTNTFSL
                     MPGVRIFDRWFDHPTRPEKGYSYLAEIRGAHRSLGSYTGFLQGTITANGIYALGGGFS
                     FLSRIKLGVTLQDEDFRELPIAIRHFAGGDNSVRGYAYQSLGVKDSRGEVVGGRHTVT
                     GSVEMEKSLFKDWGAAAFYDWGNAFNSFRDAYLAQAAGLGIRYYTPVGPIKVDLARQI
                     GTPEPVFRLHLSFGFTL"
     gene            32896..33375
                     /locus_tag="PLT63_02200"
     CDS             32896..33375
                     /locus_tag="PLT63_02200"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOD97560.1"
                     /translation="MSAFRGSDILEIAVRIEENGENFYRYATQLAKDEEAKKIFLNLA
                     EAERQHKKTFGRIFSAMEKENPPESYDGEYDAYLHDFVDNKIIFKKELIEAELAKVKD
                     TLAAIDFAIQRELDSILFYHEIKNLVPAREHGVIEEVIEEERKHFKGLSSLRTRFVK"
     gene            33520..34863
                     /locus_tag="PLT63_02205"
     CDS             33520..34863
                     /locus_tag="PLT63_02205"
                     /inference="COORDINATES: protein motif:HMM:NF005685.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOD97561.1"
                     /translation="MSRKPNEDHVFYRNLNRWYPAVQRGRGIYLYDTEGKEYIDGSGG
                     AVVAALGHGLREIEAAMLTQLGNVAFAHGSQFTSPPAVTLAEKLTAMAPAGLSRVYFL
                     SGGSEAVETAVKMARQYQVDRGKPMKFKVISRWASYHGNTLGALALSGHTGRRRYYQP
                     LIQHTPHIVPAYCYRCPCGRQPESCALECADDLEKTIIYEGPDSVSAFMAEPVVGATA
                     GALVPKDGYFQRIREICDRYDVLLIVDEVMTGIGRTGRNFAVEHWGVKPDMIVCAKGL
                     GAGYYPVFCVITTEAVHRAIKEGNGNFVHGHTYSQNPLACATALAVLDYIEKNDLVRS
                     SERLGAYLLDKLQVLYRHEMVGDVRGIGLFAGVEFVRDRKTKEPFPPARKVNALVSNL
                     AFDNGLIVYPGGGGADGVNGDHVLIAPPFIITEGEIEKLVAILDEAIGETERSLR"
     gene            34929..35747
                     /locus_tag="PLT63_02210"
     CDS             34929..35747
                     /locus_tag="PLT63_02210"
                     /inference="COORDINATES: protein motif:HMM:NF017652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HOD97562.1"
                     /translation="MDKIIITAAITGSRMMRDVAPHIPITPEEISQSAYEAWQAGASI
                     VHIHVRDPETGLGSQDIDLFRRVVEYLRERTDLILSLTTSGIPGRNLPIMERLAPLAL
                     KPELASFDAGSINLGGHAFINTPEFLDEAAVMMREAGVKPEIEIFDLGMMITTLRMRQ
                     EGKLADPLHFQFVLGTPWGAPATPKAFLHLHDYLPPEATWSNIGIGAGHLPMAMMALI
                     MGGHIRVGMEDNIYLRRGVLAKTNAELVERVVSICREYGREIATPAEARMILKL"
     gene            complement(35920..36918)
                     /locus_tag="PLT63_02215"
     CDS             complement(35920..36918)
                     /locus_tag="PLT63_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97563.1"
                     /translation="MMDQGDLFDPNYQDWNCGIADLKELRFAAAREKLHKYGESTGDW
                     ATVEVYGDIAGFLQTGLGEIDNEAPEAPEAYWELWEKLAQRAAGMKIPDPSLLNDLRR
                     NYFRKAVGVVERLGMTEHPRLPGNLPLGLLYGEAGDWDRAIASLQACLPTAANNADIY
                     AYLGDAYLRRGEREVARRCYLEACLVDPGGIDWERLMDRELISLRVYMEESGREKKGE
                     EGSDLRAPVLAWLPARAYVAGIFKPKIIRLKDEFKTFVDDFQALEKRFLKEKDHALGA
                     ALFFRGIIICDNETALRLVKGMDFADIRARMREINPPLFADYLRVIKERKKKQKPG"
     gene            37331..38623
                     /locus_tag="PLT63_02220"
     CDS             37331..38623
                     /locus_tag="PLT63_02220"
                     /inference="COORDINATES: protein motif:HMM:NF033540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS701 family transposase"
                     /protein_id="HOD97564.1"
                     /translation="MVPENRIQDGTFPIPKLFVGKEDIDQFTDEFRGFHAQFADCFSR
                     EEPRENFVQYMAGQMSQLERKSIEPIAMNVENAKVRAMQHFISNVVWDEARIISRYHD
                     MVVEDLGDTEGVLIFDESGFVKKGGDSAGVARQYCGNIGKVENCQVGVFAAYGSRQGY
                     CMLDNRLFIPEKWFGDDYAERRRKCRFPDELSFSTKPQLAVEMFEGIIRENVLPFRYV
                     VADTVYGGSPEFISAVEKITDVVYMVSLPMDTLCWVQGPSVMEKTYRYKGEERKKRVV
                     AEAEDKPITFEKLARGTNDYFWYRRTVSEGTKGHITYEFMKKRVFLSKNGRPDRNVWL
                     IVRRPIGGDAATYAYFVSNAGRSTRLKTFVWLSGVRWAIEQCFGEAKTDLGMDHYEVR
                     KFPGWHHHMITCMLAHFFLWHMRIRLGAKSTSYYTIAA"
BASE COUNT         8763 a        11644 c        10614 g         7784 t
ORIGIN      
        1 cggaccaggc aggcaagaac gccctccgtg ccctgaatat cccctgcgtc caggtgggat
       61 atctggattt gaaaggcaac aatggtaacc tggaagtcaa tatgctgggg gtgaaatttt
      121 tcgcccacag ttccggggca acgccatcgc tctttgccac caacaatgtc aacggcagct
      181 atacgaccac ggcgcccacg gcgggcacct ccgtcccact gtcggccacc gccggaggaa
      241 cgataggcac atccaccttc accgtgcaaa gctggaacag tgctaacaac ggcaaatggt
      301 cggcaaccgt ccagggggcc aatgccaaca tcggcagcac ggcgaacctc cagggattga
      361 atatcaaggg cgccgccgcc ggaaatatca ccggcacaaa cttctccggc acggccgccg
      421 gggtggcaaa gcagggtgcg ttgcccaccc cagagtaacc gcacttggcc gttttcaaac
      481 aaagcagcgg gaagcagatg atttgatctg tttcccgctg cttacatctg tggataaaag
      541 aggtaaagag acgaaaacct gatctgtgga aggaaaagta ataattgtgg ataaattgga
      601 aaaatatctc ttctgggtga tgtcatcttt cgcaatcatg tccctgacac tgcccggtgg
      661 ttgctgatca ccaggttgat ttaatccgca acgggcataa atacaaagat cgcctcgaaa
      721 gcgcctgatc tccctaatga tgatgaacga tagaatatcc cgggacgacc tccttcatta
      781 tgtcgtcaaa ccgagccgct atctcggcgg cgaagtaaac gccgtccgca aggatcatcg
      841 tacctgccgg ctcagtgtcg ccttggcctt cccggacaca tacgaagtgg ggatgtccca
      901 cctgggtctt cagatcctct acgaactgct gaacagccgg gacggcatca tcgcagagcg
      961 ggtctatgcc ccttggccgg acatggagga gctgctgcgc cgccatgcca tccccctggc
     1021 gaccctggaa tccggaacaa cgttacggga tttcgatctc gtcggtttct ccctgcagta
     1081 cgagctgtcg ttcaccaacg tcctgaccat gctggagctg ggcgggattc cccgccgccg
     1141 cacggaccgc cgggacggcg atcccctcgt tatcgccggc ggcccctgct gtttcaaccc
     1201 cgcccccatg acggccttca tcgatgcctt tgtcatcgga gaaggcgaag aggtgagcgg
     1261 cgagatggca acggtcatga tgagggaaaa ggatcgcggc tccggccggg aaaagctgct
     1321 ggccgccctt gccgaacttc ccggcatcta tgttcccgcc gttcacactc ggggcgaaaa
     1381 gatcagaaag agggtcgttg ccgagcttaa cacctggacg ctccccgtcc ggccgctgac
     1441 gccgctgatg aagaccatcc atgaccgggc ggtcctggag atcgcccggg gctgcacccg
     1501 gggctgccgt ttctgccagg cgggaatggt ctggcgcccc gtccgggagc gccaacccgg
     1561 gatcctgcag gaaatggccg accagatgct cgcggccacc ggccaggagg agctttctct
     1621 gctgtccctc agctccggcg attatacctg catcgaaacg cttttgcccc tcctcatgga
     1681 tcgttattac gcccggcgca tcgccctggc cctcccctcc ctgcgggtgg aaaccctgag
     1741 ccggaatctc atcgaggcca tccgtcgggt ccggaagaca agcttcaccc tggctccgga
     1801 agcggggact cagcgtctcc gcaacgtcat caacaagggg aacactgaaa cggacctcct
     1861 ggcaacctcg gcacaggtct tcgccgccgg ctggcgggcg gtgaaactct acttcatgat
     1921 cggccttccg ggggaagaag aaggggatct ggagggaatc gccaccctgg cccaccaagt
     1981 cctccgtacg ggaaagaaac ggggacaggt aaccgtcagt ctctccaact tcgtccccaa
     2041 accccatacg ccctttcaat ggcagcggca gatcggtccc gaggaaaccg ccctccggca
     2101 ggaattcttc aagtcccgac tccgccaccg ccagatccag atcaagtggc acgacgcccg
     2161 gatgagccga ctcgaaggcc tcttctcccg gggggacgaa acgctgggcg atctcgtcga
     2221 actggcgcac cgcggcggct gccgcttcga cggctggagc gaccggtttc gtcccgagat
     2281 ttgggatgac gccatccgcg cccttggcat cgacatcgcc gccaacttag cagcccggtc
     2341 ccggaatgcc gtgttgccct gggaccatat cgacgctggg gtggaacggg atttcctctg
     2401 ggaagagggg caaaaagccc ggggggaaga actcaccccg gactgccgcc gggagggctg
     2461 ctaccggtgc ggcgcctgcg atcatataga gatcaggccg ctcctggcgg aagatggccc
     2521 cccggaaaac catagtgcga cagccaccgg agggccggaa ggaatgacga cggaatcgct
     2581 ggtggacctg gacgtcgccg aagccacggc cacatccccg gtgacggcaa acaacgctgc
     2641 ggaaaccgga gcgttcggga tacacctcgc cgggccggag cagccggcgc gggaagctcc
     2701 ggggcaacga aacggccaga gactccggct gagattcacg aagcagggtc ggtcccgaca
     2761 cctgtctcac ctggagaccg cctccgccct gaccaggggc attgtccgca gcggccggcg
     2821 gttccttttc acggcgggct tccatcccca tccccggatg tcctttgccg tcgccaccgc
     2881 cgtcggtctg gccagcgagg gggagtactg cgatgtggta tgggaaaaaa ccacggacga
     2941 gacagcatcc cttccggaag agataaaccg ctttcttccc gaggggatgc tgataacgga
     3001 gttggtggag ttggcccccg atgccccatc cctcaccacc tctctccggg gcttccaata
     3061 cacagcagcg ctgccacccc ggctgaatgc ggcggccctg gacgccctgg agggaaagat
     3121 cgccgccttt ctcgccgcgg cggcattccc cgtggccagg accgtcaagg accacaccat
     3181 tatccgggat atccgcccct tagtgaccaa tctgattatg gaccggaccg gccgccgcat
     3241 gctcatggag gtccgcttca ccaacgaagg cgccgtccgc cccgaagaaa tcctcgtcca
     3301 cgccctgggt ttggacccat ccctgccggc ggtgatgaat ctgaccaaga cagcaacttt
     3361 ttttattgac aaataaatac cttttcgagt agattgcgcg ttcaaaattt ttcggcaagg
     3421 gtgaagcgat gtacgcagtg ataaagacag gtggaaagca gcaccgggtc tccgtgggag
     3481 acatggtggc catagaaaag atcgacggcg ccaaggggga taccgtggta ttcgacaata
     3541 tcctcatggt ggccgcggag ggacagatcc gcatcggaac cccggcggta aacggagcaa
     3601 cggtgaccgg agagatcgtc gcccagacca aggcgccgaa aatcgccgtc ttccacatga
     3661 agcgccggaa gggccaccgg aagaaaaccg gccaccgcca gccgctaacc cggatgcgga
     3721 tccgggatat ttcgttttaa ggaagggatg cgaaaccatg gcacataaaa agggacaggg
     3781 cagttcgaga aacggccggg acagcaactc ccagcgccgg ggcgttaagg ttttcggcgg
     3841 ccaggccatc aacgccggcg gcatcattat tcgccaggtg ggcacaaaga ttcatccggg
     3901 caggaacgtg gggctcggca gagattacac catctacgcc aagatcgacg gggtcgtgaa
     3961 atttgagagg ctggacaaga caagaaagaa agtgagtgtt tatgcggctt agttgtaaga
     4021 accgctttca ttgacatcct atccgctcct gaggataggg ataaagaaat aatttgagga
     4081 aaggcgcttg catcaagcgc ctttattttt attgcccatg aaattcgtcg acgaggtcaa
     4141 gatctttgtc caggccggcc acggcggccg gggatgcgtc agcttccgcc gggagaaata
     4201 cgtcccccgc ggcggccctg acggcggcga cggcggcaag ggcggcgata tcgtcttcca
     4261 ggccgaccgt catctttcca cgctcctgga ctttcgttac cgcccccacc acctggcccc
     4321 aaaaggcggc aatggggaag gcagcaaccg taccggacgc aatggggaag acattgtcat
     4381 ttccgtcccg gtggggaccg tggtaaaaaa tgccgcaaca atggaaatca tggccgatct
     4441 cgtcgaagac ggccagcgct tcatcgccgc ccggggcggc atcggcggca aaggcaactc
     4501 ccatttcgcc acctccaccc accgcacacc ccgtttcgcc caggacggca tgcccggcga
     4561 ggagttctgg atcatcctcg agctcaagct attggccgac gtcgggatca taggtaaacc
     4621 caacgccggc aagtcaacct tcatctcccg ggtgtccgcg gctaggccca agattgccgc
     4681 ttatcccttc acgaccctga cacccaatct cggtgtcgtc gcctacggac gccaccaaag
     4741 cttcgtcatc gccgacatcc ccggactcat cgccggggcc catgccgggg ccggtatggg
     4801 ggtgcagttt ctcaaacacg tggagcggac ttccatcttg ctccacataa ttgatatttc
     4861 cgaagacaat tccccgagag catcgcggct ccatgatgag gtaaaccggg agctgggact
     4921 attcaatgcc ggcctgctgg ccaaaccaca ggtcgtggcc atcggcaaga tcgacctgcc
     4981 cgcggtccgg gagcgtctga aaaaagaaat tgacatcttc cgggaaaagg gaatagaagt
     5041 atccgctttt tcggcagtta cgggcgaagg gatggaaacg gtcatgaaca gaatcgccct
     5101 cgccatggaa cgagcgtcgg caacttgttg atcgtcagga ccggcggttg atcatgattt
     5161 cgcgtcgact gaccgtcaag gcaaagggtt tttaaccaaa tattgatgaa cttgtaaaaa
     5221 gtcatcattt agacgatgaa gtaaaaagat ccagagtcgt caatattgcc tttcctatgg
     5281 gcaagcacag ggaagcatat attcaggggt tggtaacatt atgacgcgca aagagattct
     5341 caaggacgtc aaaaagatgc tcatcaagat cggcagcgcc gtcctgacgg gagacaacgg
     5401 tctggacctc cacatcatcg agcagcttgt ggaggagatg gtcgagctga agaagcgggg
     5461 ctgccaaatc gtcatcgtca cgtccggggc catagcctcg ggcaagcacc gcatgggcct
     5521 aacgggccat ctgaagagca tgccccagaa acaggccgcc gccgccatcg gtcagggacg
     5581 cctgatgcgg gtctattcca acgccttcgg aaaatacggc ctttacgtgg cccagatcct
     5641 cctgaccatg agcgacctca cggatcggaa gcgcttcctc aacatccgca atacgctcac
     5701 caccctgatg gactggggag ttatccccat cattaacgaa aacgacaccg tcgccgtcga
     5761 tgaaatcaaa ttcggtgaca acgatcatct ggcggccatg atggccaaca tcacggaaac
     5821 ccacctcctc ataaacctca ccaatacgga gggtctctat gaccacaacc ccaaccggtc
     5881 gaagaaggcc aaggtgattc ctctggtccg ggagatctcc gaggtgatcg aacaggcagc
     5941 cacggacgag acctccgacg tgggctcggg cggcatgcgc agcaaggtca tggccgccaa
     6001 gaaggttacg gccttcggca tcccctacat catcgcgccg gggaaggagc cgggcatact
     6061 ccgcgacctc ttcgccggca aggagcgggg aaccctcttt ctgcccatga aggatcatct
     6121 caacagccgc aagtactgga tcgccttcac cctccggtcc cggggcaagc tcactctcga
     6181 tgacggggcg aaaaaggccc tcatcgagga ggggaaaagc cttctcccct cggggatcat
     6241 tgccgtggag ggggatttcc atgtgggcga ccccgtcctc tgcctggaca ctgcggggaa
     6301 ggtactagcc aagggactga ccaattacgg agcggaagag ataggcaaga tcatgggtct
     6361 caagacttcg aagatccagc aggttttggg ctacaaggat tacgacgagg tgatccaccg
     6421 ggacaatatg gcggtggaca aagagctcca gcacaaataa ccggcggcca tccccgtcga
     6481 ccggacgtta tggcgccgac cgcgtcttca tgatccagcc gcgggcacac cggagccccg
     6541 ggcactgccg gatcgatgga aaacatcctc ctgggcgcaa cttctccgcc ggaggtttag
     6601 agcttgttcg taaataagct ctaaaccgat tatccctgtg atcggcctcg gggcgccgga
     6661 acctgtattc cctcccacgc cagcaggacc tttttcatcg ccagaccgcc gccgtagccc
     6721 cccagcgtac catcgccgcg aacaacccgg tgacagggaa tgacgatggg gaagggattg
     6781 ccggccatga cacggccgac ggccctggcc ccccggggcg atccgacccg ttccgccagt
     6841 gcgccatagg agatgacagc gccctggggg atccggaagc aggccgccaa cactttcttc
     6901 tcgaagtccg tgcaaaaagc catgtccagc aggtcgagag agaaacggac cgcttgtccc
     6961 ttcagggcgc gttggagatc ggcgcagaac aggcggatgg ccggggaatt ctcccggacg
     7021 atgccgggga accgccgttt ccaaacccct tccggaccgt ttttttcgct cggcaggatg
     7081 attgccgcca ccttcacccc tccttcttcc cctccgaccc gccagacgac ggcaacctcc
     7141 ccccagggag aaggaaccga ctcatatttc caagaagcaa ccataaacca tcctcccacg
     7201 ctctctttaa agtgaaatga cgaaaaaagc taaataatcg gatccatatc cccgctcgga
     7261 ctcctccatt ttcaggccag gccatcccag aggatcttca ggccgatccc gatgaggatg
     7321 aggcccccca acgcctcgac gcgccggccg aacatctttc ccaacctttc cccgaagagc
     7381 atcccgaccg ccgtcatcac aaaggcaacg attccgatga ccgcactcgg atagagaatc
     7441 ggcgtcttca tgaccgccag gctgaggccc acggccaagg cgtcgatgct cgtcgccacg
     7501 gaaagcatga tgagggtgac gccccgagtc ggatcctgcc ccggcgccgg gtcctcctca
     7561 ccgcggaaag attcgtggat cattttacct ccgacataga ggagtacggc aaaggcaatc
     7621 cagtgatcat aggaatcgat ataggcggcg atggtcacgc cgcccagcca gccgacgatg
     7681 ggcatgaaaa actggaagat gccgaagcta gccgacaggc gcaggatcga cgttaaggag
     7741 acccgatggc gggtcgcccc gatgcccacg gccacggaaa aggcgtccat gcccagggcc
     7801 acggcaatca acagaatggg ccataaattc gtcaagaagg tccctcatcc aattttacgg
     7861 gtggagattt cgacattctc tcaataagga tcatccaccc cgtccgataa agcaaaccgg
     7921 ggcgatcttc catatcattc gtcgcctccg tcacccgtcc gcttccattg tccgcttcgg
     7981 acgggaggga agtgtttttg gtgtcttacg gcttcatcat atctttatgg gccgtaaaaa
     8041 atcctcgatt cttgacgcca gctttttgcc tatccctgga attacccgca attcctcggc
     8101 cgtcgccgtc cttatccgtt ccagatcccc cattcccctg aggagggcct ttttcttctc
     8161 cgggcctagg ccggggatat catccagccg cgaggcaaaa tcctcctgtt cccggacccg
     8221 ccggtgatag gttacggcaa accggtgggc ttcatcccgg agccgctgga gaaggtgcag
     8281 gacggcggga taccgggaga gaaacaccgg ctccttccgt ccgggaagga aaatccgatc
     8341 gctgatcctg ccccggccga gcttcacgtt ttcgtcctcc gccccgaccg tctcctttcc
     8401 catcgtttcc gacctgacca tttccatccc acccgtctcc tgatctgcgg caccgtcggg
     8461 atgttttaca tcggatcgaa aagagtgccg gcgtcccctt tccgtcccaa gcgacgatgt
     8521 ctctcccgcc ccgccagtgg cgccggtctg gccttcccta ccgaggaggc cacgacccct
     8581 gctacctggt gggaaatccc gttctttggc cagggcgatg acatccaccg accctattcg
     8641 cagatcctta aggaccgaca aggccacggc aagctgtccc ttacccccgt ccaccacgag
     8701 gagatcggga agatctttcc ccgtggcgaa acgtcggctg aggacctcgt acatcatggc
     8761 gtaatcgtcg gcgccgggaa cggtgcggat gcggaaacgg cgatagtcgc tccgccgggg
     8821 gacaccgccg gcaaaggtca ccatggaacc cacggagtat cggccgccga tattggagat
     8881 atcgaaacat tcgatccgct ccggtggccg ccggagttcc agagccgccg ccagggcctc
     8941 caggtctccc ccggttccct gatgccgccg gacgacctgc ataatctccg tggcattgcg
     9001 tccggccatc tccaccaagg cccggccccg cccacgctgc gggacgaaaa gacggacgcg
     9061 ccgcccccgc ttctccccca gccattccga caggacccgg cggtcctccc ccgccgcggg
     9121 caggaggatc tccccgggaa tttcattacc ggtgtcatag aactgcatca ccaccgtggt
     9181 catgatctcc ttcatcggca ggctgacccg gagcggtggg aaggtccgca tccccaccat
     9241 cttcccggcc cggatccgca gcaggcagag ctgcgtccag tcaccttccc gggcggcgcc
     9301 gaagacatcc tggtcctggg ccgcgatcaa ggcggcctgt tgcttttcca gagtagtccg
     9361 gatggcgccg atacgatccc ggagggcggc ggcctcctca aaccgaagtt ccgccgctgc
     9421 attctccatc ctttcctcca gttccttcag caccgccgtg gcgtttccct ccaaaaaggc
     9481 cagtccctgg cggacggacc gccggtaggt ttcctcagcg accagatgga cgcagggggc
     9541 cgggcagcgt ccgatctgat attccaggca gggacgccgg cggtttcgga gttccacatc
     9601 ccggcatgtt cggaggggaa agacggtttg cagaaagcgc agggtctctt tggcggcgcc
     9661 gccggagggg tagggtccga aatacctcgc cccgtctttt ttcggacgcc ggaccaattg
     9721 ccagcgggga aaggggaccg cggtatccat acgaatgtgg aaataggcct tgtcgtcacg
     9781 aaaaacgacg ttgtaccggg gacggtgttc cttgataaga ttgttctcga ggatgagggc
     9841 ctctttttct gtcgcggtaa tgatccattc cacctgccgg acccgggata ggaggaaggg
     9901 gatcatcccc ctgctatccg ttccaccggc atagttacgg aggcgggccc gaaggtcttt
     9961 tgccttgcca acgtaaatga tctccccgac gccgtctctc atcagataga cgcccggaac
    10021 ccggggggcc acctggacga tatccgccag acatgtcccc gtcatcgccg ctggctccat
    10081 ttccgaaccg cctcgtctgc ccgggaagat gacgattggt cggcaatccc caccggcgct
    10141 ggccggacgg atctcaacgc gggaaaagat aacaccgaaa atgatataga aaaaaagctc
    10201 aatctgaaga tgtccaagct cataccgatt ctcccccgaa ctccaccagc aggctgctca
    10261 gatccctgat ttcatcccgg agttccgccg ccttttcgaa ctccagcgcg gcggcagccg
    10321 ccttcatctc cgccttcagg cgggcgatca gcttcgtcag atcctcttcg ccgccatagg
    10381 ggactttcgt ctcccgcacc agcggcacgg ttacataatc cgcgtcataa accgaggtca
    10441 ggacatcaga gatggacttc cggatcgact cgggggtgat gccgtagcgg cggttatagt
    10501 cggtctggag gaggcggcgg cgctgtgtct cgtcgaggca ggcccggatg gatccggtga
    10561 tcacatcggc atacatgatc acctgtccgg cgatatgccg ggcagcccgt cccgccgtct
    10621 gtatgagcga ccgctccgaa cggagaaagc cctccttgtc ggcatcaagg atggcgacaa
    10681 gggatacttc cgggatgtcc aggccctccc gaaggaggtt cacgccgatg aggacgtcga
    10741 attctccccg gcggagatcg cggatgatct ccacacgttc cagcgtatgg acgtcggagt
    10801 ggagataacg caccttcacc cccacctcac cataatgggt cgtgaggttc tccgccatgc
    10861 gtttcgtcaa cgtggtgacc aggacccgct cccctcgcgc cgcccgttgt cggatctctc
    10921 cgagaagatc atcgacctgg ctcttgacgg gcttgacgac gatttccggg tccatgagtc
    10981 ccgtgggccg gatgatctgc tccacgaccc cacccgacgc cttcatcagt tcgtaagccg
    11041 ccggagtggc ggagacataa atgcgccgct ggggaaaggc ctcgtattcc tcgaagcgga
    11101 gaggccggtt gtccaaggcc gagggaaggc gaaatccata ttccaccagg gtctccttcc
    11161 gggagcggtc cccacggtac atgccgatta gttgggggat cgtagcatgg ctctcgtcga
    11221 taatgatcag ggcgtttttc ggcagatact ccatcagggt gggcggtggc tggccgggat
    11281 ttctcccggt caggtggcgg gaatagttct cgatcccctg acagtagccc atctcctcca
    11341 tcatctccag atcgaaaagc gtccgctgct ggagacgctg ggcctccagg agtttctctt
    11401 cccggcgcag ctcggaaagg cgttcctgga gttcggccct gatagtggtc atggcccgat
    11461 tcaggttgtc cctgctggtg acatagtgac tgccgggata aatggccagc ttcgagattc
    11521 ttcctaattt cctgcctcga aggggatcga cgtagaggat ctcctcgatg gtatcgtcaa
    11581 aaaaggccac ccggacagcc cgctcatcct cataagcagg aaagatttcc accacatctc
    11641 cccgcacccg gaatgtcccc cggtgaaagt cgaaatcgtt gcgctcatac tgaatctcca
    11701 cgaggcggcg cagcatggtg tctcggccca actctcttcc ttcctccaga tagaggagca
    11761 tcccttgata cgcctccggt gaacccaggc cataaatgca ggagacactg gccacgataa
    11821 tgacgtcttc ccggtcgaag agggcatgag tggccgcgtg gcggagcttg tcgatctcct
    11881 cgttgatcgc cgagtccttc tcgatatagg tgtcggtgct gggtatgtag gcctcgggct
    11941 ggtagtaatc ataatagctc acgaaatact cgacggcatt ggcggggaaa aggctcttga
    12001 actccgcgta caactgggcc gccagggtct tgttgtgggc gatgaccagg gcgggacgct
    12061 ggacggactg aataacgtga gcaatggtaa atgtcttccc cgacccggtg acaccgagca
    12121 gtacctggtg ggggagatcc gcctttactc cttgcaccag ccgggaaatg gcccgtggct
    12181 ggtctccccg gggcgaatat tccgatacga gctcaaaggc cttcatcagc gccttttatc
    12241 atatcaggcc gggaaagcga agcttcttgt tcataaagcg aagcttcttg ttcataaatt
    12301 gatcttgata ttttttgtat atctgttagt cttttacttt cctacattgg aacgatatgg
    12361 taagaagttg cgaggcacta cgccttggcg cccggcggca tacgtgccga aacattcctg
    12421 agaatcatag atcgtccact caccaaacaa ggagggaaaa aacatggaag ccaccaaaga
    12481 atccgcccat gccagtgtcg acagtttcat ccatgcccgt aaggccaaat tcaccatgaa
    12541 cctctcacca gtaacgcagt tgctggcctt tcttgactgg ttcatcaacc ttgccgactc
    12601 accggggaaa cagtaccgga ttatggagga tttctgggaa aaaacggcca attactggga
    12661 ctatgttctc cgttcctgtc tgctttccaa tggagaacct gaaaagccca tcgcccgcga
    12721 tccccgtttt cgggatgatg cctggcagac cccccccttc aattgcttcc atcaggcctt
    12781 cctccttagc gaacagtggt ggcagaacac cacaaccggc atccctggtg tttcccatca
    12841 tcaccagaat atcgtttcct ttatggcccg gcagattatg gatgtccttt ccccggccaa
    12901 ctttctctgg accaacccgg tcttgaccag ggtaacggaa gagcaagggg ggcaaaacct
    12961 catcaggggc tgggaaaata tgctggagga ctggcggcgc cagcgtaaca acgagctccc
    13021 cgcaggcacg gagaatttcc gccccgggga aaaggtggcc gtgacacccg gcaaggtggt
    13081 tttccggaat cacctcatcg aattgatcca gtatacaccg acgactaaaa aggtttacga
    13141 ggaaccggtc ctcatcgtcc cggcctggat catgaagtat tacatcctcg atttgtcccc
    13201 ccacaattct ctcgtcaagt tcctggtgga caaggggcat acggtcttca tcatctcctg
    13261 gctgaatccc acggaaaagg accggaacct ctccatggcg gattatcact tcgacggtgt
    13321 aatggcggca ataaatgcca tcgccaagat agttcccgac aggcagatca acacggcggg
    13381 atattgcctc ggcggcacca tgctcgccat tgtcgccgcc acgatggccc gggacggcga
    13441 cgaacgtctg aagagcatga ccctgctggc agcgcagacg gacttcacgg aagccggaga
    13501 gctgagcctg ttcatcgacg aaaatcaggt aaacttcctg gcggacatca tgttcgaccg
    13561 gggctatctg gacaccaagg aaatggccgg cgccttccag cttctccggg ccaacgacct
    13621 gctctattcc cgcatcattc aggactacct gctgggcgcc cgccgtcccc tgaacgatct
    13681 catgtcctgg aacgccgacg ccacccgcat gccctaccgg atgcacagca aatatctccg
    13741 ccgtttgttt ctggataatg accttttcga gggtcgttac ctcgtcaatg gccgcaatat
    13801 agttatcagt gatattcgcg ttcccgtttt ccttgtgggc accgtccagg accatgtcgc
    13861 cccctggcgt tcggtttaca agttccacct tacatccgat gccccgacgg taaccttcgt
    13921 tctcaccagc ggcgggcaca atgccggaat cgtcagcgaa ccggaaaaca cccgccggac
    13981 atatcagatg cttacccatc acgaggggga taaatacatc gatcccgacc gctggctgga
    14041 ggaagcgccc aagttcaagg gctcctggtg gctcccctgg caggaatggc tggccgccaa
    14101 ctcctccggc atgacggttc ctcccggtat gggtgccccg gataaaggat tgaaaatatt
    14161 gggagatgct ccgggtactt atgtccgtca gatctaatca ccgcgaccgt tttaacttga
    14221 caagcggcgc cgaatatggt tatttacttg cctttacatg aacattaact taactgaggc
    14281 ttgaaagacg atgaaaaaga cactgaaacc cgtgagcaac acgcgccgga aaaggaccca
    14341 tggattcttg gtccgcatgg ccaccaaagg aggaagactc gtcctcaatc gccgccgggc
    14401 aaaagggcgg aaaagattgg ctgtttaatc ctggcaaaga caggccgtcc attcccggga
    14461 atgcccggcc tgcacatccc atggaacgtt cgaccttcgg caaacaggaa aggataagga
    14521 aacgaagaga ttatttgacg gtctatcagc aggggattcg tgatcattcg gggcatttta
    14581 cctgtctaac atgccggaat ctctcaggga aaagaagact tggcatcacc gtgggcaaaa
    14641 aggtgggcaa ggccgtcaag aggaaccgga tcaaacgtat aattcgggaa ttttatcgtg
    14701 tgcataagca gaggcttccc gcctctctcg atattgtgat catggcgaag ccccaggtta
    14761 acatgttgac caatcacgag atccggaggg cgctggaacg gctttttgat agaataaagg
    14821 atgaataaca aggtattatc gcaaacccta gccaaaatat tggtcctggt tattcgctta
    14881 tatcaaatct ctatctctcc ggttttgcct ccctcctgtc gtttttttcc gacttgttcc
    14941 gaatatgcca tccagtccat acggcgttac ggccccctac ggggaactat cgccggcctg
    15001 aagcgtctcg gtcgctgcca cccctggcac cccggtggct acgacccggt cagataattg
    15061 caaaaaggga ggtaatgcat ggacaagcgg accattctcg ccatcgtcct gtcggtaacg
    15121 gtcatcctca tttaccagat gttctttatg aaacccccgg tgaaagaacc ggcgcccccg
    15181 caggagacag cccaaacgga aaaaaaggac gttccggcgt cggcgccgcc tcccaccgct
    15241 ccttccgccc cgacacagat caaggcgggg aaaggcatcg tgccgccggc cccagtggtg
    15301 gcggaacggt tcattaccgt ggatactcct ctttactccg cccagttcac cacgaaaggc
    15361 ggggcgctcc attccttcaa actcaaggga taccgccaaa aactggttgc gtcttcggaa
    15421 cctatagaaa tggtcgatgt cgcgccgggc atgccccgtc cgttgacggt gtctttcccg
    15481 gggtcctccc tcgacccccc cggtgagggc atgtatgaat cggaaacgaa aagcctggct
    15541 ctgaccgaca ccaaggaacc tcagcgcctc gtgcttcggc atactgtagc caacgtaatg
    15601 aaaatagaaa agatttttac cttctatccc gaccgctacg ccatcgacct ggaggtacgc
    15661 acttttaacc tcaacgccgt tcccctgagt cagaacgcca gcatcaactg gaatcagtac
    15721 gccgatccga aagaagagac ggacagttgg tcccacaaag gcccggtggc cttcgtcgcc
    15781 aaggacatcg agcggatcga tgtgaagaag atcgaaggtg gcaaactcct gggacccaat
    15841 gttacgtggg gtggttttga aagcaagtat ttcatcgccg ccgttattcc ccaaaatcct
    15901 accctcacca gtttggctgt cgccaaagat ggtcgggaca tggtctcggt cagcctgcag
    15961 ggtccgaaaa tcctcgttcc cggcggtcag gccgactcct tcatctatac cctttttatc
    16021 ggtcccaagg attactccat cctcaaggtc cagaacgtcc atctggaaaa cgccatcgat
    16081 ttcggctcct ggctgaaatg gctggccatg cccctgctca ttctcctcaa atggctctac
    16141 cagtacgtcc acaattacgg catcgccatc atcatcctga cgatcctcat caagctcatc
    16201 ttctggccct tgggcaacaa gagttacgaa tctatgaaga agatgcagaa actgcaaccg
    16261 aagatgaagg aattgcagga gaaatacaag ggagaccggc aaaaactgag ccaggaaacc
    16321 atggccctgt accggacgca caaaatcaac cccatgagcg gctgtctgcc catgctcatc
    16381 cagattccgg tgtttttcgg tctctacaag actcttcttt atgccattga actccggcat
    16441 tcgcccttct tctggtggat acaggatctt tccgagaaag atccctacta cattacgccg
    16501 atcatcatgg gaggcaccat gtggctgcag cagaagatga cacccgtagg gggcgacccc
    16561 atgcagcaga aggtcatgct ttggatgccg gtaatcttca ccttcctctt cctcagcttc
    16621 ccgtccggac tggtcatcta ctggctattc aataatatta tcagcatcgg acagcaatat
    16681 tacatcaaca agcgtcattc ttgaacgagg taatcgatga aaatgatcga attcgaagga
    16741 aaaacaattg atgaagctat cgaaaaggcc tgccgggagc tgggagcgcc ccgggaaaaa
    16801 ctgaatgtcg aaatcgtggc ggaaggaact tcaggattct tcggtctggg gtccaaacgg
    16861 gcaaaaatca aagcccgcct catggatata aatattgacg aggaacatgt cgtcgatccg
    16921 atcgccgtta cagcaagatc caaggccccg gaaacaaagg acgccccatc acatcccccg
    16981 acccgggccg ccgcggaagc tgccggcgcc aacctggccg caaaggagga caaaccgctt
    17041 cggaaatatg cgcctccggg agcgtcatcg ccaacggatc aggaagtggg cgccagggcc
    17101 caggccattc tctccggcat cctggagcgc atgaatattg cctccccggt tgccgtggat
    17161 atccgggagg atgccatcgt cctgaacatc aagggcgaca gcggcggact gcttatcggc
    17221 aagcggggcc agaatctgga tgccctccag tacctggtga acaaggccgc caaccggtcc
    17281 ggtgaagagc ggaaaatgat catcatcgat accgaagcgt atcgccagcg ccgggaggac
    17341 gccctggcat ccttggcgga acggctgggt cagaaggtca aaaagacgag aaaagccgtc
    17401 accgtgagcc atatgaatgc ccatgaccgc cggatcatcc acctaaccct ccaaggggac
    17461 tcctccctcg tcaccaaaag ccgcggcgag ggggagtacc ggaagatcat catcatgccg
    17521 gccagaaagg aaagggagcg ggaccggggc gagaccacca cgaccacggg agtcaggtag
    17581 ggcaaccatg caggcggacg acaccatcac ggccgtagcc actcctccgg gagtgggagg
    17641 catcgccgtt atccgtctca gcggaccggc cgcggaacag atcggcaaga cgatcttccg
    17701 cggtgcccat aaccacccgc cgaaattcac cacccaccgc ctgtaccacg gccatatcgt
    17761 ctcggccgtg gatagcgccg tcatcgacga ggttctggcc gtccttatgc gggcccccca
    17821 ctcctatacc ggcgaggatg tcctggagat tcacggtcac ggcggctatc tccttcccca
    17881 acggatcctg gcggaaacca ttcgagccgg cgcccgtccg gcggcaccgg gggagttcac
    17941 gaaacgggcc ttcctgaaca atcgcctgga tctctcacag gcggaagcgg tggcggacat
    18001 gatcaacgcc cggacagaac gggggctgga gcttgcccga tctcaatacc agcgccagct
    18061 cacggcagag ataacggccc tgcgagaggg aatcgtcgac gctatcgccc gtcttgaggc
    18121 cgccattgaa ttcgccgctg acgaccccga tatcgccgac gacatgtcga tccccaacca
    18181 gcttcaggaa ataggtcacg aagtcaactc cctcatcacc acataccggg aaggcaagat
    18241 ctaccgccat ggggtcagtc tggtcatcgc cggccgaacc aatgtgggta aatcgagcct
    18301 tctcaaccac cttttgggag aaaagagggc catcgtcgcc gccagccccg gcacgaccag
    18361 ggatttcatc gaggaaacgg tcaatatcca tgatatcccc gtgcgtctga cggataccgc
    18421 tggtatccgc agtaccgccg acgccgtcga gcaggaggga attgcgatgg tctggcagcg
    18481 tattgacgcc gccgatgcgg ttctgttagt tttcgacggc tcggaaaatc tgacgacagc
    18541 ggaccggcaa attgtcgcga agctggccgg taaagccctg atccccttgg tcaacaaggt
    18601 ggatctaccc cggatgctgg cgccggaagt cctatccgat ctcctcccga accatcaggc
    18661 gatctggata tcggcgaaac acggaaccgg gatcgatgag ctgcttcagg caattcatca
    18721 ccttttcctc gccacggaaa gagagacttc cgccggtacg gtaataacct ccctgcgtca
    18781 caagctggcc ctggaaaagg catccttttt catctgtcag gccatggcgc gggcagcccg
    18841 ggggggggca cccgagttgg ccgccacgga cctccaggat gccctggaag ccctggaaga
    18901 gatcaccggc aggactgccg acgaaacgat cttggaaagg atatttgcca atttttgcgt
    18961 tggtaaatag agactttatt ccctttacaa agttttctcc cttcagtata ttcagcccgt
    19021 aaaaaatcgc tccggaggtt caaggttatg ccaagatcaa cggctgttat ctgcgttttc
    19081 tgcgctgcct tattcatcct gtcctctcct tccgcctggg ctcaactccg atgcgttgac
    19141 gccgaaggtg aggcggcgat catgaataat gaccgtccct ccgcaaggat ggaggccgtg
    19201 gctcgggcga agtggcatgc catcgagcag gccctcggtg tcgatgtgaa agccgggagt
    19261 tatgtatcgg acttcgccct tgtggaggat gtcatgaaga cccaggtggg gggatcggta
    19321 aaaaaattca ggatcttaag ccagacagaa aaggacagcg ttctcaccgc tcggatcaat
    19381 gcctgcgtgg aaccgaaaaa ggcccaggag gccatcgcct ccgaactggc cctaaacagc
    19441 ggtatcgccg tcttcatccc cgcccggaaa ccccaaggaa ccaccggaga ggatgtcttt
    19501 gaagagacta atatcctctc ggaaaatctc atcggcagac ttacggagca gcactaccgg
    19561 gtcgtcgatg tagcccccac ccatgccctg gccgcggcgg agatcgaaaa ggccatgcgg
    19621 agcggctcta ccttggccgt ccggagcatg ctctacaaat ttctatccaa tattgccgtt
    19681 atcggacgga ccgactatac cgtatcgacg aaaaagggcg aagatatcgg ctacggcctt
    19741 tacatgcctt tcaacaatgt cacggtccga ctcacctacc ggatggtcgt ccggaacaaa
    19801 aaaaccggaa acatggaaat actcagcgcc ggtacggaac agggaaaggg catccgcaac
    19861 agcgttgagg atgccgcggc ggtggccctc aaggatgttg cggacaaggt gatcccgaaa
    19921 atcctcgaca aagccgccca atatctccag ggaaacgtga aacgcatccg ggtaaagatc
    19981 gccggcgtca aagatatcga aaccaacatc gatatcaagg gcatcctcca gaacatcgtc
    20041 tgggtgtcgg aagtggaaga gctgaagatg ggtgagttca tcgtcgccta tccggaaaat
    20101 tccgtatatc tggccaacag tataaaacag aagggtaatt tcaccgttga caacttcact
    20161 ccctattcgt tgaccctcag ttatcgaccc tgaagagcaa tctatgaagc gggctggcct
    20221 ttccggtttg ctgcttttcc tgatcctctc tggctgcgcc tcggcgccga agctcgacct
    20281 caccaccttc gtgacggacc gggaagccgt gatccgcgtg cccgacatct gcaaacccag
    20341 atacgacgcc gccctgccct atgtggccgt cgtaaatttt accaacaata caaccttcga
    20401 ttatgtccgg atcatgcaga gccggattca ggattcgggt tttcaagccg atgcccagag
    20461 catctcccgg gaggtaaatg ccaaactcgg cgaaagcatc gaagccggcg tcaccgatga
    20521 gattgtcaac acggggggtg cgaaggtaat cacccgcagt gagatgaaca aggtcttcga
    20581 agagcaacag tttcagcagt ccggtctggt ggacgacgcg actatgatcg atatcggaaa
    20641 gatcaaaggg gtcaaataca tcgtcaccgg ctcgatcaac aacgtggaca tgcgatggat
    20701 ctctcaccct tccacacggc agggactggg gaaaggcgga agcggcagct gggccttcga
    20761 cctcatcggc gtcgccatag ccgcctccct ggaagcccag gagggatggc atatcggtgc
    20821 cgaggtcacc ctgcgcatcc tcgatgtgga aagcggcgaa gtcctcctct cgaagaaaat
    20881 gaacggccgg cataacatcg gcaggatacc ctatcccaat tacgatgtcc tgatcggcgg
    20941 catcaagaag gccgccggca aaagcctaca ggcagcccgt ccggaattgt ccaagtattt
    21001 ccccctcaag ggttatataa ccatgatcaa gaacagtccc gaaggaaaaa agaaggccgt
    21061 ccggatcaat atcggagaaa gcgaggggat cagaccggga gacgagctct tcgtctatac
    21121 ctttgaagag gtggatgatc ccatgtcggg cgggaaggaa tgtgacatcc agcggcttcc
    21181 agtgaccctg gtcgtgtccc gtaaccagtt gcggcccgat gccgcctggg cgctggtaaa
    21241 ggcaaagaca ccggagcagc ttcgaacaat cagggtggga caactcgtgg agagaaaaac
    21301 tcaacgctga cggagaatct atgagataac ctatcgatat atcaaaccat ccaacccacg
    21361 gttagaatca gcgccaccgc aaataagata gctctccctg gattcgctca cctcaccctg
    21421 ggagagctat ctatgcacct gcactttcca tcccttcgat cagggctagg acgttctgcc
    21481 ccaggacccg gtgattatat cccccttcca ggatggcaaa gtatccccct ttgtttcttg
    21541 ccgccgcctc ccttaccaat tctcccatct cccggtaatc gccggtctgt aaaagtccac
    21601 cccagtcatc cacgtggttg tcgaagcctg cggagatgcc gataatatcg gcacgtcctc
    21661 cttcgagaaa ggaccgtacg ccctggagat aatcccggcg actggatgac gatgggttgt
    21721 agatattgac atagcctttc gaacccagga tgtttaccgt accgtcaccg aagtggagat
    21781 cgaagtcgag gaccatggcg gtgttgatca ggccgtcgcg cttgagtttc tccaatgcga
    21841 tggccatgtt gttgaaatag caaaaacccc aggaactgcc tgccgaggcg tggtgacccg
    21901 gcggtctgat cagggcgaag cacggttcct tcatcccgat ttgtgccgcc tgcacggccc
    21961 cgcccgccgc cagggccgca atgtcataaa tgccctcgcg tcgcaccatc tccagatgat
    22021 tctgggtgtg aacggcggcg atatcttctt cagcagccgg aacggcatta atgatcttca
    22081 ccctgtcctt gattacggaa taaaccgcct cgatgcgacc aaaggcggca gccggatcgg
    22141 aacagtaaga aatgtcgaac tccttggaat aaataacttt catggcatca cctaaatacg
    22201 ttgtagggac gggaccttgc cgccctcatt tccgcttcgg aaatatcccg aatattcacc
    22261 ccggtaaaca ccccatcttc gtccctttcc atgacccgga attgttctgg gggcgtcaac
    22321 cggttattac ggcggtcaat tccttcccgg taaaccgtca tacttctttt gtacacctct
    22381 gtttcctcca aacatagcac gatgggtttg ttgaatccct gaaagtggaa ccgtacaaac
    22441 gaccatcgcc gccgctaaaa aaagatccgg cgcttcattg cgaccccctt ccctccatac
    22501 ggataaaaat gttctacatc atccggcatt aattcgtgct tacatgggaa aacctatttc
    22561 tcttgggaga tttataaaca tgaatcacct ttggtgcttg aatcgtttgt gggtgttgga
    22621 taaacatgtc aacccattaa gtcaaaaagc cccggctgcg aagaatcttc agggtatttg
    22681 ccgatacggg caggtccggc aattccgccg gggaaaccca gcgggccgcc agggcgtcgt
    22741 cggccgcctg tacctcccct cccagatatt ccgcctccac atcgacgatg acaaaatggt
    22801 actggagacc ctttcggtca tcatactgaa tgaagtcgaa tgcataaacc ggatttcccg
    22861 ccttgatgcg gatgccggtt tcctccagga tttcccgttc ggccccttcc tggatggttt
    22921 cacccgcctc cagggaaccg ccgggtatgg cccacaatcc ttggctgggg ggagccgccc
    22981 gctggatcag cagcaccttg ccgtccttca agacaatggc cccgattccc acaatgggtc
    23041 gctgaggata ttcccggcgc gtcatatttt cctccttcag ttttccagga cgaaaccggt
    23101 cgacatccaa agtccgtccc cgcccgcgta ttgccgctcc tcaaaatgaa tccgattctg
    23161 gcggtccttg aataccacgg tggaagacct cgtcccgtaa atcggactgg caataaagat
    23221 cgttgcgagg atccgttccc attccagacc aaccccggta tcgggcagtt cctcgtcacc
    23281 cggctgcgac cggtcagcaa gaagggcaaa cagatcttcg gtatgcagac gccccgccct
    23341 tgtcattacc cgacgaaact cctctttggc cttaatcacc ttcggccacg aggaatcgag
    23401 gagattattg ctgagtccat atatgcccgg aggcaggagg aatggtgcgc tcccccggtt
    23461 ggaataataa tacaaatcgt cctcatcgcc caagaccaga ctaaacccgt tgtaacgtcc
    23521 cccctctttt tgcagatccc ccagatattc cgctgccccc ttctcgccgg taaggaaatc
    23581 gcggaccagc cagccccggg aaggggcatc cacattgacg tgccgtggat cgcgataatt
    23641 ggtgagggcg gcaaaatgtc ccttgcgtgt tatccccagc caggtccctt tttccttcaa
    23701 atcccgaccg ccaaggatct ccggatggtc atcccaaaaa tcggcggcca aagtcgggcg
    23761 atcataaaat tcatcccgat tggcggccac tacgagggga taatcggcac gctgccgcca
    23821 ggcgaaaatg atcaggcaca tagaccgccc gtacgcttca agtttgccac gattcccgtc
    23881 atcacctcgc cggcaccgcc gcgaatgacc agatcagcgt tggcatcata agaagtttcc
    23941 gtcctgttga tgatcatcaa tttggcccca ccacctttgg cgtacagggg aatataggcc
    24001 gccggataga ccaccaggga agaacctatg actatcagga gatcgcagtt gctggcggcg
    24061 tcactggcct ccttaagcgt ttgcggtggc agggcctcgc cgaaaaagat gacatcgggc
    24121 ttcagcagcc ccccgcaacg ctcgcagccg gggatctcgc cgctttcccg gtacctcgcc
    24181 agcatgaccg gcaggggata acgttcaaga cacgccaggc acttcatcca cttcatgttg
    24241 ccgtgcaatt cataaacccg ttccggagag ttaccggcct gctggtggag atcgtcgatg
    24301 ttctgcgtga tcacgcagac gagtttaccc tgctcctcga accaggcgat ggcccgatgg
    24361 gcgtcattgg ggcgggcctg ggcaaaaatt cctccctcga agaggaactt ccactgtttt
    24421 tttctcgttt ccggactgga gaggaatcgc tcgatggtaa aatcatcggg atcgaacctt
    24481 gaccacagac cgccggggct ccggaaatcg gggataccgg attccgtgct gactccggcg
    24541 ccggtaaaca cgacaatctt ccctgcggcg gcgatcattg ccaccgcttc attaatcatg
    24601 gtctccaaat cgatgtcgct catgagcgat ctcctcacag ttggaatttg ggacgacttc
    24661 tacctttaag atacactatc ttccggaaaa atcaaaggac gatgttaaaa ccccttccgg
    24721 aatggactga tcaaataggg tcttgacata aaaggaacaa cctaatactg ttcagacgtt
    24781 cggcgatgcc actggaggca ttccattcat tgccgaaaca caggaaatgg atcattgatc
    24841 ttaatgcagc cccttctcac cttcgtcaac aaccttcgca ttcaggatgt ccttgacgta
    24901 atcatcattt ctctgatgat ctacttcctc ctggtgtggt tcaaaaaccg ggcctcccgg
    24961 tttgtcctca tcggtctcag cgtcgtcggc gccgtttacg ttaccgcacg gttctttcag
    25021 ctttatttga ccaccgtcgt cctccaggga tttttcgcca tcctcatatt tgtcctcgta
    25081 gtcatcttcc aggaggattt gcggaggctg ttcgaacgcc tggccattat cggcagattc
    25141 ggtggacaat ccaccgccag caccaagcgc ggagatacgg aaatactggc ggcaaccgcc
    25201 accaatctgg cccgaaacca cattggcgca atcatcgtcc tgaagggaaa agaccccctg
    25261 gagagacacc tcggcagcgg taccgccctg gacggtctga tcagccagga gctgctggag
    25321 agcttattcg atccccattc ccccggtcat gacggcgctg tcatcatcac cggcaaccgc
    25381 atcacccact tcggctgcca tctccccctg tcgacgaata tgggacgcaa cggtgccacc
    25441 ggcctgcgcc acaccgccgc cctgggtctt gccgaacgcg ccgatgctct gtgcatagtg
    25501 gtttcggaag aacgggggag tatctctctg gcccggaacg aaaagatacg ggttttaagt
    25561 agtggattgg aattgcgcaa tgaactggag aaattctacc gggaactggc gccagagaaa
    25621 cgaactgcct cccgcatctc cttggaactg cggaagaaca cccgggagaa gatcatcgcc
    25681 gtggccctgg catgcatcct ctgggtaacc tttggctacc agaaagaaac ggtccgaaga
    25741 gaatatctcg tgcccataga acatctcgac ttttcccgcc atttcgtgct ggaagaaccg
    25801 cagacccggg aggtgaaggt gatcttatcc ggcaccaccc aggcctttca gctcctggat
    25861 tcgggcagtt tgaaaatttc tctcaaaaaa gcggggatcc gggagggccg acagagcatc
    25921 gtcctcacga gcgatatggt aagaactcct tcgaacatca acgtcgccgc catcatccct
    25981 ccggaaatca cggtaacggc aacccggtat ctacccgtct ccattcccct ggaggtgacc
    26041 accacgggga aaccgccccg ggggatagcc gttcagaaaa tatccgtgga tcccgccgcc
    26101 gtgcgggtct tcattcccgg cgtcgccgtc ccaggaaaga tcaagatcca gaccgaaccc
    26161 gtcgatctcg cccgcctgtc cgaacagacc agcttcgatg tcacgttgcg ttatcccccc
    26221 aacgtcaagt ttcccgacga caaagttccg tccacggtcg taacggtcaa gatcaggaag
    26281 aaagcggggg gcggcgacca ggacagatga ttccaccgtg cagcggggcg aggccgcggg
    26341 aagaggtttt ttaaccgaaa tcatgaccaa caacgggttt ttcacctgaa aatgcgttcc
    26401 tatcttcccg acgtcactca caaatatttt tccccttcaa aggaagtatt aaggtgggga
    26461 tgggtattgt attttatgaa tattaagcta cgctttcatc ccccttggtg acgggacacc
    26521 tttatgggtg tttcaccgga acagaggaga tccggccttt caatcgaact ccagcttgta
    26581 gaagagatcg atgctgctct ggcggccggc cttcgtttcc acctcccaga gtctcgaaaa
    26641 ggaatatttc atcacgtaga gaatgtcatc gctgaacatg gcccgggcca gccccacata
    26701 gagctgtggc gacaggtact gccccatggt ggatagggag gtggtcatgt cggttctccc
    26761 ctgcttgctc cgggacgggg aggttatccc gcccacctcc agaccggcgc ttttgcgcag
    26821 gctgtcctcc agccctcccg actttccctg ggcaagaaac atccccgctg atttcagcag
    26881 tgtggtggcc tgaatcttat ctcccgtata gctcgtccca aagacgatat aggccagaac
    26941 atcctgatcg ggcagggagg ggacggaata aagggtcgct accggcgccc gcagcgtccc
    27001 cgtcaccaga aaaccggccc gaacgtcacc gacctccttg accgccagga tgtccagggt
    27061 ggccagggag aagggcttat ccttgaagaa gatatgtcct ctttctatgg gcagtttcgt
    27121 attggccgca tgcagatacc cgtgcgtgat atgcatttca ccccgggccg gcagatcttc
    27181 caatttcttg cccgaagcgg tcatttcccc ggagaaattc ccctcgatat ctctggtcct
    27241 catatagacc tgctccccca aagaaatggc caggtccatg tcaacaacaa aaggtacggc
    27301 cggagccttt tttaccggct tgtcgattat cttcacatct ttgctggcgc ggatcagatc
    27361 cggttcccgg ggctcgaggt agcggccttc cgggacgtgg atcgcccccc ggacagagag
    27421 gtactccctg gtcccctcga aggtgatacg gggactgacc agcaactgca gttccggaag
    27481 ataggaggcc tggaatcgct ccccctccag gtggcctttg atatccttga tgttaccgcc
    27541 accatagcgg atcgtcgccc ggccctttag gtttcccggt ccggaacggg cggaaaatcc
    27601 ctctatttct atcctctccg ggaaaaacct ggcctccgcc tccatatctc ggagacggat
    27661 ccccgttgcc gttacgtagg cccccgcctt taagaggcgt agatggcctt cgtaacgggg
    27721 attctgccag acaccggcaa cggccagatc gacggccagc cgaccgtgag tctcccggac
    27781 cgtccccgga aacacagcgc ccaggattcc cttttccctg atttctccct ttaccgaggc
    27841 gcttatgggg ccttgagggt ttattcccac tgggtattgc gaccggaccg gcagggaaaa
    27901 aacggcccgc aaatggccat gatcggtaaa tgccccctcc atggtccccc gcaggatgtc
    27961 gtcccgccag gtccaaccga gggccaggcg ttcctgaacg aaccgtacca tcccccggcg
    28021 tccggcaacc gtccatatgc ctttcacggc ctccgcttgt ccggtcatgg caaatcgtcg
    28081 ttgtggcagc agccttcctt cgagacggcc cgagaggatc ccctcagtgc gaagggagga
    28141 agggagaaag gggttcaaaa gctggaggtc aagatcggcc agactcgcct catagctgcc
    28201 ggaatcgggg aaagaaaaga aaggcgcgga tgatgacgac aatcggacgt tcaatctgcc
    28261 catgaggccc aaatcgaaac tgctgctcac ctccagtccc ttggcctgcc attggaagcg
    28321 taattgtcca tcctggacct ccagacggtg ttccccccgc acgatccgcc cctggagccg
    28381 tgccgttccc gccgcctgga atccctcccg gaccccgcgc tgctctatcc gcacctgacc
    28441 ggtcgtcttg ccgttcaaat gcatatcggc cgtgggcaag ggcaaacggc ccagaggaaa
    28501 attctgccag gaggccgtgg catatccggg ctgttttttg tcgcccagga gagcggaaat
    28561 ttccaatcgt tccccctgcg gtccggccat gataaagttc tctatcctca gcaggtgcgg
    28621 ggatacggac agggacgccg gccgggaaag ctgaaggaca ccgtatgatt tgctgtccgc
    28681 cgccgcggtg cccacagccc ccgtccatac tccccgctca tatcctccgt ccgcccgtac
    28741 cgccaatcct ccctccggac ccgtgagccg caggttcaac tcctgactct ttctcgttcc
    28801 cgtcaccgta aactcaccct ggaggcattg tccggctaca tacaggtcct tggccgagat
    28861 ccaggcccgc agttctcccg cctcgccttc acccagggac aaagacaacg ccaggtcatc
    28921 tatccggtcc tgatcccagg atatcttctt acctgcggcg gtcagcgcgc cggaaaggag
    28981 acggtttcga taccgaaccc agccgcggcc atacagccgg ccgtcggcct ggggaataag
    29041 gcgggaaatc ctggcaatcc gaacctcaaa gccgatccgg tcccggagat ctccccgagc
    29101 ttgagcttga aagccttctc cccgtacctc cagtttttcc agaaagagcg cgtccccttc
    29161 ccagcgcatc ccgaccccgc cggtcatttg ccgcccggac aggcggctgt cgagaaaacg
    29221 ccccgccagc ttgccgcttg tctctccccc ggccccacgg cgccatattc cgtcgagatc
    29281 ggcattgatc accccccggg cctgcgacac cgccagagaa ggatccatat tccgtgcccg
    29341 caatgcccag gaaaccgtcc atcccctttc ccaggcaacc gttgcccgcc cgcgggcgat
    29401 cccttggccg acatttatct gcaacccatc gatggcaatc cgttccccag cgccggaaaa
    29461 actgccccgg accgccagcg gcggcccggc gacgaggcgg ggccgcagat ggaaacggcc
    29521 ctcataggag gacaaattgc cggcaagctc caggtcgccg ccaagaacca gttcccgccc
    29581 gacttccgga gacaggtcga gacgatcgat cttcaacgtt gaggacaacc gcactgcggg
    29641 tcgggaaaaa tcgaggcgcg cctccccttt caaagcaccg gcccggttct gttcctctcc
    29701 ggacagtccg gttagggcca ggatatgggc ggaggtggcg gcctgacctt tcaagatgaa
    29761 gcgttcccgt ttcccatcga caatcacgac ggaaacagga ccctgaaggt ggagagaatc
    29821 ggcagatgcc cgcagggacg cccgcaaggt cgtctccagc catcgcccgt catcgggagc
    29881 aaactttaac tgcgtctcca gaaaaggact ggtaagatgg agttgtaggc gtccggacag
    29941 ttttccgccc gggacggacg cagcgagatg ggaaagactc gccagtccgt acgtgtaatc
    30001 caacgccgcg gccatatggg gtatagttat ttcccggtcg gcacttctcc agaggatatt
    30061 ccggacatcc aggacgtcga tgcccccgcg gaaccaggaa aggaagtcgg gcaggcgggg
    30121 ccaggttaga tcgaggggtg tggccggccg gtcgtcctga aagtccactc cgtcgagcat
    30181 cagctttccc agaaatacct tccccgccca caattcccag ggtcgccact ggaccaccgt
    30241 ccgccgcacc atcaggcgcc atccctctcc cccgacctgt aaatccgcca cggacagttc
    30301 cgtgcccagg gctcccgaga tttgccccgt ctccacccta gccgggatca tccccaggag
    30361 acggccgacc agccagcggg acccgttttc gctaccgaag agccatgccg tcgcccccaa
    30421 aattgtgagg atgaccgccg ccaccaggaa aacagctaaa aacctcttcc cggcatgaag
    30481 agaaagcatg cggttttctt cgctgcggtc atcgttcgaa ggcggggacg gaaagcggct
    30541 gttcatcata acgtaaaccc gaagcttaga tggaggcgga ataccggttc cggtgttccg
    30601 atctgtcggg ccagatcgac ttttatcgga ccgacggggg tatagtaacg gatgccgagt
    30661 cccgccgcct gggcgagata cgcatcccgg aagctgttga aggcattgcc ccagtcatag
    30721 aaagccgcgg cgccccagtc tttgaacagc gacttttcca tctcgacgct ccccgttacg
    30781 gtgtgccggc ccccgaccac ctccccacgg ctgtccttga ctcccaggga ttgataggca
    30841 taaccccgga cgctgttgtc gccaccggca aaatggcgaa tggcgattgg aagctcccgg
    30901 aaatcttcgt cctgaagcgt aacgccaagc ttgatcctgc tcaggaaaga aaagcctccc
    30961 cccagggcat agatgccgtt ggccgtaatg gttccctgga gaaatccggt ataggaacca
    31021 aggctccggt gggcgccccg tatctccgcc agataggagt agcccttttc gggccgggtc
    31081 gggtggtcaa accagcgatc gaaaatgcgg acgccgggca tgagggaaaa ggtgttggtc
    31141 tgggcgtcgc cggccgtgga atcctcccgc aacagctgga gaaagacgga ccccgttttg
    31201 tccaagaaat agggccggac atgctggagc tccatcgaga actccttggt gtcgtatgtg
    31261 tcgggattgt cccgcttgag attgaccccc agggaggaaa aggtccggac atcccgtccc
    31321 ggaaccgtat agcccagggc caagccctgg aaaacgcggc ttacattgat tcccccttcc
    31381 cagtaatggc cggcatcgat aatattttta tcccggtaat tgatggatgc ccggggaccc
    31441 gtgtcggtgc cgtagccggc accgagtttc aggtgtttct ctggtccggg ccgtagcttg
    31501 atctccacgg gaacccggtg gtcccgggcc ttttccttat ccgtctcgat acgcacatcg
    31561 gcgaaacgat cggccagggt gagattccgc tgcgtctccc cgattttttc gtaagagaac
    31621 acctctcctt cctggaaggc cagataccgg ttgagaaacg gccggggata cagcggagcg
    31681 ccggagaaac ttatcgcacc gaaaaaatag cgggaaccgg tgtcatagac gatctcgata
    31741 tcggcggtcc gttccccgag gttaacccgc acctcctgaa cgagaaagtg gccgtcgagg
    31801 tagccccggc ggatggcgac atcgtgaatc ccccgctttg cctcttcata aacctcatga
    31861 cgcaatacca tccccggagc gaggggaaag tttgccacca ggggacgaaa gtcctcctca
    31921 tcccttcccg ttccttcaat acggacggcg ataccccgca ccaggaccgg gtcccctctg
    31981 gtcaccgtga cctgcacctt catgtggcct tccggcgccg cttcctggga tactgtcgcc
    32041 gtcccgtgat aatagccgaa tggctccaga gcttcctgca ccttgcgggg aatctggtcc
    32101 agaaaatgat ccagccaggc ctcgtcgatt cgatccttct tgacgagacc cggcggcacg
    32161 acaagggccg tctggacatt tttcagttcc ttgccttcca gaccctgaac aagcacttcc
    32221 acgggggcgg cagcccaggt caaagaggcc gccagcaaga cgatcaggaa tggaataagg
    32281 catttataag gatccaaaca ttcccccggc agcgctgaaa aatcaaacca cggcatgcat
    32341 gacgaacatg cgggaaggct atgaacgtta agcttctctc tgctctttaa tcttaatatc
    32401 gttcataaaa caattgtttc cactgccgcg gtcatgagaa aaaggtcaaa tgtaactttt
    32461 tcgaactcat aaatacttgt accacggttt tacctactgc cgccgatttt ggtattatac
    32521 ttgtcgatat ttgtaaagta ccgatcggga catggtaaat aagcgagcat tcttttttcc
    32581 cctgcaagac ctttggcagt tgacttcccg gtgggcaatg cataagtctc ccggtagggg
    32641 cgaacgggga ttgagctatg gaaagatgtt gcatgccagc aagataaagg caggcataac
    32701 gaccagttat taggtccgtg gggaaggtca cacaatggtc accacggcca tgggcatgta
    32761 tttcaagaga aaaaatgggt tgttaacggc cgtgttttca tttatatagc gttaatatga
    32821 agttcggggt gaatttcaca agatctttat aactttagag gtaaaataga aataatgaat
    32881 tcggggaggt aacagatgag cgctttcagg ggcagtgaca ttttggaaat cgccgtgcgg
    32941 atcgaagaga acggggagaa cttttaccgt tacgccaccc aattggccaa agacgaagag
    33001 gcgaagaaga tcttcttgaa tctagcggag gcggaacggc agcataagaa gaccttcggc
    33061 aggatctttt cagccatgga aaaggaaaac ccgccggaga gttatgacgg agaatatgac
    33121 gcctacctcc acgatttcgt ggacaacaag atcatcttca aaaaggaact gatcgaggcg
    33181 gagttggcca aggtgaagga caccctggcc gccatcgact ttgccatcca gcgggaactc
    33241 gattccatcc tcttttatca tgagatcaaa aatctggttc cggcccggga gcacggtgtc
    33301 atcgaagagg tcatcgaaga ggagcggaag catttcaagg gcttgtctag cttgaggact
    33361 cgcttcgtaa aatagggagt cagttttatc cagccggcgc ccccaggcgg caccggctcc
    33421 ggccaagggg atgaggacgg aaacacgaac ctcccggcgc ggtgtaaagt tgcgccccgg
    33481 acacctagac agtaataaat agggagcgac aggaaaacga tgagcagaaa accgaacgaa
    33541 gaccatgtat tttaccgcaa cctgaaccga tggtacccgg cggtgcagcg cggccggggg
    33601 atttatctct acgatacgga agggaaagag tatatcgacg gctcgggggg ggcggtggtg
    33661 gccgccctgg ggcacggcct ccgggagatt gaggccgcca tgctgaccca gttggggaac
    33721 gtggccttcg cccacggatc ccagttcacc tccccacccg ccgtgaccct ggctgaaaag
    33781 ctgacggcca tggcacccgc cggactgagc cgggtctatt tcctctccgg ggggtcggag
    33841 gcggtggaga ccgcggtgaa gatggcccgc cagtatcagg tggaccgggg caaaccaatg
    33901 aaattcaagg tcatctcccg gtgggcaagc taccacggta acaccctggg ggcactggcc
    33961 ttaagcggcc atacggggcg gcggcgctac taccaacccc tgatccagca caccccccac
    34021 atcgtccccg cctactgcta ccgctgcccc tgcggccggc agccggaaag ctgcgccctg
    34081 gaatgcgccg acgacctgga aaagacgatc atttacgaag gccccgactc ggtttccgcc
    34141 ttcatggccg aaccggtggt gggtgccacc gccggcgccc tggtccctaa agacggttat
    34201 ttccagcgga tccgggagat atgcgaccgc tacgacgtcc ttctcatcgt cgatgaagtc
    34261 atgacgggaa tcggccggac cggtcggaac tttgctgtgg aacactgggg agtgaagccc
    34321 gatatgatcg tctgcgcgaa gggactgggc gcgggctact acccggtatt ttgcgtcatc
    34381 accaccgagg cggtccaccg ggccatcaag gagggaaacg gaaacttcgt ccacggccat
    34441 acttacagcc agaaccccct cgcctgcgcc accgccctgg ccgtcctgga ctacatcgaa
    34501 aagaacgacc tggtgcggag ttcggagcgc ctgggagctt atctgctgga taaacttcag
    34561 gttctctacc gtcatgagat ggtgggcgat gtgcggggaa tcggcctctt cgccggggtg
    34621 gaattcgttc gggaccggaa aacgaaggaa ccattcccac cggcccggaa ggtgaacgcc
    34681 ctggtaagca acctggcctt cgacaacggg ttgatcgtct atcccggcgg gggcggcgcc
    34741 gacggcgtca acggcgatca tgtcctcatc gccccgccct tcatcatcac ggaaggggag
    34801 atcgagaaac tggtggccat cctggacgag gcgatcgggg agacggaacg ttccctccga
    34861 taaattctgc ggtacggcgc cggcaataaa ttcggggccg cgcccctgct tttccaagag
    34921 gcagcaacat ggacaagatc atcatcaccg ccgccattac gggcagccgg atgatgcggg
    34981 atgtggcgcc ccatattccc atcacccccg aagagatctc ccagtcggcc tacgaggcct
    35041 ggcaggccgg ggcatccatc gtccatatcc acgtccggga tccggaaacg ggcctgggca
    35101 gtcaggatat cgatcttttc cgccgggtcg tggaatacct ccgggagcgg accgatctta
    35161 tcctcagcct cacgaccagc ggcattcccg gccgtaacct gcctatcatg gagcgcctcg
    35221 cccccttggc gctgaagccg gagttggcct ccttcgatgc cggttccatc aatctgggtg
    35281 gccatgcgtt catcaatacc ccggagtttc tcgatgaggc ggccgtaatg atgcgggagg
    35341 caggggtaaa gccggagatc gagatcttcg atttggggat gatgatcacc accctgcgga
    35401 tgcgccaaga gggaaaactt gccgatcccc tccatttcca gttcgtcctc ggcacgccct
    35461 ggggcgctcc ggcaactccc aaggccttcc tccacctcca cgactacctg ccgccggagg
    35521 ccacctggtc caacatcggc atcggcgccg gccatctccc catggcgatg atggccctca
    35581 tcatgggcgg ccacatccgc gtcggcatgg aggacaacat ctacctgcgc cggggggtct
    35641 tggccaaaac caacgccgaa ctggtggagc gggtcgtgag catctgccgg gagtacggcc
    35701 gggagatcgc tactcccgcc gaggcccgga tgatcctgaa attataacgg ctgcccggcc
    35761 gccgcaagga gagagattac ggcttcagat catttccaga gcgagccaaa atttttacga
    35821 atcgtcggct attaaacaat atcaatatct tacaaactgc ctgatttcgg cggcaccggg
    35881 aggaaatcga ttccgcccgc tccgagatga tctctttgtt cagcccggtt tttgtttctt
    35941 cttccgttcc ttgatcaccc ggaggtaatc ggcaaacagc ggcgggttga tctcccgcat
    36001 ccgggcccgg atatccgcga agtccatccc tttgacgaga cgcagggccg tttcattgtc
    36061 acagattatt attccccgaa agaagagggc cgcccccaag gcatgatctt tctccttgag
    36121 aaatctcttc tccagcgcct ggaaatcatc cacgaaggtc ttgaactcat ccttaagcct
    36181 aatgattttc ggtttgaaga tcccggcgac ataggcccgg gcgggaagcc aggccaggac
    36241 cggagcccgg agatccgaac cctcttcacc cttcttttcc cgtcctgact cctccatata
    36301 gacccgcagg gagattagtt ctcgatccat cagacgttcc cagtcgatac ctccggggtc
    36361 taccagacag gcctccaggt aacagcgccg ggccacttcc cgctcgcccc ggcggaggta
    36421 ggcatcgccc agataggcat aaatgtccgc gttattcgcc gccgtgggca gacatgcctg
    36481 gagggaggca atagcccggt cccagtcccc cgcctcgccg tagaggaggc cgaggggcag
    36541 attccccgga agccgggggt gctccgtcat ccccagtcgt tccacgacgc ccacggcctt
    36601 ccggaaataa ttgcgccgca gatcgttcag caacgatgga tcggggattt tcatgccggc
    36661 ggcccgctgc gcgagttttt cccacaactc ccagtaggcc tcgggggcct ccggggcctc
    36721 gttatcgatc tcccccaacc ccgtctgaag aaatccggcg atatccccat agacctccac
    36781 cgtagcccaa tctcccgtcg attccccgta cttatggagt ttttccctcg ccgcagcgaa
    36841 acgcagctcc ttgaggtcgg cgattccgca attccaatcc tgatagttcg gatcaaaaag
    36901 atcaccttga tccatcattc ttcattcccc aaacagtgat actaatattt tttcaaaaaa
    36961 agtctctgcg ccctactgct gcattgggtt tcgttttcac ttaataatat catacagtta
    37021 aaccctatcg gattaagata atttttagtc ccctatttcc gataacagtg agcagaagga
    37081 ttgccgccaa ataaaattgc caaggaatca gtattaacaa ccaattcagc ggacaggaaa
    37141 ctttactgcc gcaagaaatg cccggggaaa gaagcctttg catggcgcat cactacatta
    37201 aaatatgtca tttttatatt agcgattgaa aaggatcgta atcgccacaa cgatacataa
    37261 gccatttata agttgatacc atattccgac tatgctacat ttttataaaa gcattgggag
    37321 gtgtcagaaa atggtaccgg aaaaccgcat tcaggatggg acctttccca tccccaaatt
    37381 gttcgttggc aaagaggaca ttgaccagtt cactgatgag ttcagaggtt ttcatgccca
    37441 gtttgcggat tgtttttctc gggaggaacc tcgagaaaac tttgttcagt atatggcggg
    37501 ccagatgagt caattggaga ggaagtccat tgaacccatt gccatgaatg tggagaacgc
    37561 taaagttcgt gcgatgcagc attttatcag taacgttgtt tgggacgagg ctcggataat
    37621 ttcccgatat cacgatatgg tcgtggaaga ccttggggat accgaaggtg tcctgatatt
    37681 tgatgaatcg gggtttgtga aaaagggtgg cgattctgca ggggttgccc ggcaatactg
    37741 tgggaatatc ggcaaagtgg agaactgcca ggttggggtt tttgccgcct atggttcccg
    37801 ccaaggatac tgtatgctgg ataatcgctt gttcattccg gagaagtggt ttggtgatga
    37861 ttacgcggag cgtaggagaa agtgcagatt tcccgatgaa ctttctttta gcaccaaacc
    37921 acaactggcg gtggagatgt tcgaggggat catccgtgaa aatgtccttc cctttcggta
    37981 tgttgtagcg gatacggttt atggaggcag tccggaattt atatctgctg tggaaaagat
    38041 taccgatgtg gtctatatgg tttctctgcc aatggacacg ctctgctggg tacagggacc
    38101 gtccgtcatg gaaaagacct accgctacaa aggagaagaa agaaagaagc gggtggttgc
    38161 ggaagccgaa gataaaccga tcactttcga gaaactggcc agggggacta acgattactt
    38221 ctggtatcgc cgtaccgtct ccgagggaac gaaagggcat ataacctatg agtttatgaa
    38281 gaagcgggtg tttctttcca aaaacggcag gcctgatcga aacgtatggt tgattgtccg
    38341 tcggcctatc ggtggggatg cggccacata cgcctatttt gtctcgaatg ccggccgatc
    38401 cactcggttg aagacctttg tctggctttc cggagtacga tgggccatcg aacagtgctt
    38461 tggagaagca aagacggacc tggggatgga ccattacgag gtcagaaaat ttccggggtg
    38521 gcaccatcat atgattacct gcatgttggc tcacttcttc ctctggcata tgaggatcag
    38581 gctgggggca aaaagcacca gctattacac tatcgcagct taagctgctc atcagtgccg
    38641 tgctacctat gaaagtcttc actgttgccg aactcattaa ccttgtggaa tggattcagt
    38701 tgaaaaacta tcgggcatat ctgtcccata gaaaacggaa actgacaata atgatgaatt
    38761 cgtaaaaagt catgatggag acgacgaagt aaaaagctcc agagg
//
LOCUS       DAOSMF010000008        38717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_1409_length_38717_cov_21.055921, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000008 DAOSMF010000000
VERSION     DAOSMF010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 38717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38717
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1409_length_38717_cov_21.055921"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1207..2985)
                     /locus_tag="PLT63_02225"
     CDS             complement(1207..2985)
                     /locus_tag="PLT63_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOD97565.1"
                     /translation="MRQYFKQMDNLGKPLKPAFKEMMTGNVAKIKEIMAEIDREVDRI
                     KNFGVSTETLHQRGEMTVWERIEYLVDPGTFTPLHTIYDPDDEESGQTNVVDGLARIN
                     GKWCVLVGFNNKWIAGAWIAGQADNNLRVTDMAKILHLPLVWLVNCSGVKLPEQEKVY
                     ANRRGQGTCFFRHAELEQEGVPVIAGIYGTNPAGGGYQGISPTILLAHKKCNIAVGGG
                     GIVSGMSPKGSFDVEGAETLIEATRHFKSVPPGSVAIHYNETGFFKAVYETETDVLDG
                     IKEWMNYLPAYNPKFFRVAEPAEPKLSAADLESIIPVNTREVYSFDEMLARLTDNSEH
                     MEFQPGFGPEMYCGLVKVDGFLMGVIGNRQGLIPKYPEYTNEYGAIGGKLYRQGLIKM
                     NEFVTLCGRDRVPMIWFQDTSGIDVGDIAEKAELLGLGQALIYSIESTELPMMLVVVR
                     KGTAAAHYVLGGPTANNNNAFTLGTPATEINVMHGETAAAASYARRLVKEKDAGRSLD
                     GTIQKMNDLVKHYEEKSRPFYCAKKGLVDEVVRYEDIRKYMIAFANAVYQNPKSICPQ
                     HHMLLPRMIRSQIVKGLERPPKEKKQ"
     gene            complement(3037..4200)
                     /locus_tag="PLT63_02230"
     CDS             complement(3037..4200)
                     /locus_tag="PLT63_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOD97566.1"
                     /translation="MNFALPEELEMLRGMVRDFAAEKIAPFADEWDEKHYFPYEEAVK
                     PMAELGFFGTVIPEEYGGNNMGWLAAMILTEEIARASSSLRVQVNMQALGCAFTIFRY
                     GTEAAKKKYIQKLVNAEYMGGFAITEFNAGSDVMSMKSMAEDKGDHWLLNGSKTWISN
                     ASVADSIIYYAYTDRSKGSKGLSAFVVELKNFNGIRTTDLDKLGSRSSPTGEIYLDGT
                     KVPKENILGNPGDGAKIVFGSLNQTRLSAAAGGVGLAQACLDSVLKYANEREQFGKPI
                     GQYQMIQDMIAQMVCEIEAARLVVYKAAWQKDQGNLNNTLEVAQAKYLAGELATKCSN
                     YAMRILGAYGYSTEYPVARYYRDAPTYSMVEGSANVCKFVVALDQLGAKKAKK"
     gene            complement(4297..4728)
                     /locus_tag="PLT63_02235"
     CDS             complement(4297..4728)
                     /locus_tag="PLT63_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HOD97567.1"
                     /translation="MAKKEMDNRFSKFGTVSFTAVTRVNDFIGYLEQGKLMGTKCKKC
                     GLKFFPPRADCYKSLDSDMEWFEVSGTGKLLSFSTLTFAPTGFTQDLPYTIAVLDYGD
                     YKVFGRVDSSIPDSELSVGMAMKAVVHPLPNDRMNYVFQKG"
     gene            complement(4730..5896)
                     /locus_tag="PLT63_02240"
     CDS             complement(4730..5896)
                     /locus_tag="PLT63_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HOD97568.1"
                     /translation="MSKVGIIGVGQSKFVRAYPGSVRELVFEGFKEAMEDAKVTTKDI
                     DATIVCSAPEYDKQRSPSGVFAEYLGLNPQPTFYVESLCSSSTTGLRLAYSLVKSGLH
                     DMVMVLGFQKMSEISAAESQERMGRGTDIQWEAPFGTMMPAYYAMFAKGHMAQYGTTP
                     EDLALVRIKASTYGILNDRAMNRKVLDMDAFKAPAPLAGILSTPLRVGDCCNNADGSS
                     CVIVANEEKAKAFSKKPVWIKGLGSATAAVNMAGRETFYSLPAAAEAARQAYGMAGMK
                     PGDINVAEVHDCFTIAEILAYEALGFAKPGEGPALIRAKETYKEGRIPVNVDGGLLSK
                     GHPVGATGGSQVRTIVLQLRGEAGDIQVKDPAIGLVHNVGGVGLYANVTILGRE"
     gene            complement(5926..6708)
                     /locus_tag="PLT63_02245"
     CDS             complement(5926..6708)
                     /locus_tag="PLT63_02245"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOD97569.1"
                     /translation="MSYSTIVFEKSVEEKLGIITLNRPEVRNAINPAMREDLSRCFLE
                     LEADTDVRAILITGGPKVFAAGADIAAMVEKTAVEQFSRASLWDLTFRMEQSRKPIIA
                     AIAGFCLGGGCELAMACDVRIAAESAKFGQAEINIGIIPGGGGTVRLTRLVGLGKAME
                     LVLTGRMISAAEALRINLVNEVVPDEQLMAEAKKMAQVISRHSPVALGLAKYAVQNSV
                     GADLATGRAIENTCFSLAFASEDKTEGMRAFLEKRKPFYKGK"
     gene            complement(6727..7608)
                     /locus_tag="PLT63_02250"
     CDS             complement(6727..7608)
                     /locus_tag="PLT63_02250"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HOD97570.1"
                     /translation="MEVKKVMVVGSGTMGAGIVQVAAQSGYDVVMKLHRNMERGMNLI
                     NKGLDFLVKKEKITAEDKQAVLGRIKLTTDWEDAKDVDFVIESIAEDFASKKETFEKL
                     DKICGPDTIFASNTSSLAITKLATCVSRPDKFIGTHFFNPVPAMRLVEIIRGLKTSLD
                     TIETAEKFIHSLKKETVRVKDVPGFLVNRINQAFRNEAYHCLSEGVASIEDIDKAAKL
                     ALNHPMGPFEVADLVGLDIGLSVLKGLYDSYRDPKWWPDMNLEQLAESGDLGRKTKKG
                     WYDYSSGEKKPRTDVEF"
     gene            complement(7714..9549)
                     /locus_tag="PLT63_02255"
     CDS             complement(7714..9549)
                     /locus_tag="PLT63_02255"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOD97571.1"
                     /translation="MEFKYDMKDLKFILKEWLPSQEVLECDRFKENFSLDDIDILLNE
                     AYKVACEIVSPINAKGDAVGAKFVDGKVTPAPGYSDAYRFIQQNGWGSSSECIELEGG
                     MPLIIYKAVHEMNTSACPALTSYVKLTSGAANLIIRFGTKEDRALFIPKMLSGDWQGT
                     MCLTEPSAGSDVGDAITRAIPTDDPMIYKIKGTKMFITAGDSGICENTIHMVLARPEG
                     GMSGSPGLGLYIVPRIWVEKDGTLGKANDVTTMGIEHKMGLKAQATALLSFGENDACY
                     GIRVGPPPDKNGASRGLAMMFHMMNESRIGTGHNANCQATAAYFYAAQYAVERIQGRP
                     FGEKNAERVPIIKHEDVRRMLLDLKAHTEGIRAMIFKGFYYLDIQANSKDVERAKMCG
                     ELAEIITPLVKCYGSETSLNLVAEAIQIFGGVGYTSEYPVEQYLRDSKILTIWEGTSF
                     MHGNDLVGRKMRMKDGKPFSLWLSTMKGFIEANLGADGFHDEMMNLKKGYDCLEEIKS
                     IYDSWYSDFETKKSLIPLYAIKALFVCAQVQVAECLMEQALIAKRRLSELPDNHYDRT
                     FYEGKIASARYYAEQVLPQAYMNTDIIKKAGKTVMECPEAALTIG"
     gene            complement(9722..11368)
                     /locus_tag="PLT63_02260"
     CDS             complement(9722..11368)
                     /locus_tag="PLT63_02260"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOD97572.1"
                     /translation="MAIESNVTKEQIEYYTQLGFWDKWTFCDYVKRWAAAFPNKEALV
                     DNRTRLTWSQYKTMMDRLALNFLELGIKRDDLVVTQIPNCVEAIITEMALARIGACVV
                     PLAMQWREHELNYVLGQTGSPAVVIAKEYGKFNYVNMMKGLQETNPGLKHLIVLGDDA
                     PEGTVSFQKLWENRLEEKYAADYLDKNFKIDPNDVLTMCFTSGTEADPKGCPRTHNHW
                     KAFERSAFLHHLTENVHDKVVMALPWINLFGQSVGVLPMIMVGGTLILLDGFNPATMA
                     QTIAKEKATLYAGVPAMHIGLLNYPELDKYDLSSLRVVASGGAPCPTTVIKQMMAKFG
                     CLVWNGFGSNEGHLNVTEVGLEPELVSTTIGLPQLYSQTKIVDENGNILGAGKVGEFC
                     QKAPFVIAGYYKRPDLNANKWDKDGFYHTGDACYLDEDGFYHFSTRLKDIIIRGGMNI
                     SAEDVEFILYKHPKIQNVAVVGMPDAKLGEKCCAFIELKANETITLEEVQAFMNQEGV
                     AKYKWPERIETITQLPRTPTGKVLKYVLRDSIAQKMKNPQ"
     gene            complement(11464..13110)
                     /locus_tag="PLT63_02265"
     CDS             complement(11464..13110)
                     /locus_tag="PLT63_02265"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOD97573.1"
                     /translation="MGKGVDYSILKELWEKNYTITTQLYRNLQKWPDKPAIIDPLKRT
                     MTFRQWDEEANQFAHALLDAGCTTFDTVMGDLYNCCEWFTLYMGAAKARCIFASQNFM
                     LPEGQVSRLMDDSEVKVFVYDAGMKNMVVNAVALCKTKPRVCVMVGAGEVPAGHVSYE
                     KFIEGKPKTAPATEKDVKMFDPVLLLYTSGTTGLPKGFTLSQACIFFDCMMNAGLHKI
                     DSQCVSLATNPLFHRGGNTTGPVCLLHCGGSVVIMKAFDDNLALDYIEKYKVSHMVSA
                     PVIYERMCQTQEKQPRDVSSLRALISMGSPLDKESCLRCMKILCPGVYNGYGTADQHW
                     VTMLKPWELPERAGTIGLAITEDLIKLVRIDLGRRGDPNNPADDCPKDGKTEGEIALQ
                     TMHGCYGYINRPEDEAKGLLYRGWQLPGDTAIWDKDGYITICGRVDDMIITGAENVHP
                     VIVEDALKDHPGVADVFVTGAPSKEWGETIVAYVALKDPNVTEKDLEEFCKNSPRLAR
                     YQRPRYYKFVDIKELPFNPAGKKLHYLIKERAKKDFVDLA"
     gene            complement(13272..14441)
                     /locus_tag="PLT63_02270"
     CDS             complement(13272..14441)
                     /locus_tag="PLT63_02270"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOD97574.1"
                     /translation="MDFEFNEEQVALRDMVRRFTEKEVAPYYTQWDREKKYPKHINKK
                     IGELGLIGMTVPPEKGGMGAPYVTEGMVCEEVSRGDCSITMSTFVVGHLDAEILAQGN
                     ETLRKDFLQPFLAGDKIPAFAITEPGSGTDAVAMKATAIKKGAKYILNGEKAGITAMM
                     DADFAVVFAKSDPAAAGAKGVSCFVVPTDYPGVTLQSYEDMGVRSVARGSIFMEDVEI
                     PDYYLIGKEGRGFAMGMEGFDISRILLALEAIAPAMVSLEETIEYAKQRNTFGRPIAT
                     FEGVSFPIVEHISILEAVRLLCYKGLWMRDQGKSSTKIAGMVKWMAPRFSTNAIRDCL
                     VLHGHPGYTNEFPFEQRLRDVLAIEIADGTSQVSKLVALRELFGREYLPYTYRNK"
     gene            complement(14487..15239)
                     /locus_tag="PLT63_02275"
     CDS             complement(14487..15239)
                     /locus_tag="PLT63_02275"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF009466.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HOD97575.1"
                     /translation="MKLKDKAAIVTGAGQGIGRAIALELAGEGAKVCVADLNPETAKK
                     TAAEIKAMGMQAIAVTADITDLSQCEKMVQATIAEFGCVDVIVNNVGWDKMEPFIKSE
                     PTTWDKVIAINLRGPINCFKAVLPVMIERKYGRIVSIASDAGRVGSSGEAVYSGAKGG
                     IIAFSKTVAREVARYGITINCVSPGPTDTPLFAAVAGDNPAIAEGLKKAIPLRRLAQP
                     EDIAGAVAFFASDKASYVTGQVLSVSGGLNMV"
     gene            complement(15287..15697)
                     /locus_tag="PLT63_02280"
     CDS             complement(15287..15697)
                     /locus_tag="PLT63_02280"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOD97576.1"
                     /translation="MIKGIDHIGIAVKSIDETLGFMKDAFGAEEVKRIDYPQLQQVSS
                     SVKIKDTTFELMQPTGPDGAIAQFMKNSPTGGFHHISVLCDDLEKLVGELEGKGLKII
                     GKMYDGPDRVAFIHPKSGKGLLIELTDTGTYGKK"
     gene            complement(15727..17268)
                     /locus_tag="PLT63_02285"
     CDS             complement(15727..17268)
                     /locus_tag="PLT63_02285"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOD97577.1"
                     /translation="MGVKELIEDLERIEKQVQAGGGQKATDDQHKKGRLTARERIDLF
                     FDKGTFVETNMYSQHQCHDFGMEKKRPYGDGVITGYGNVQGRPVFVYAMDFTVLGGSI
                     GLSNASKIAQAQKLAAKTRVPMIGLVDTSGARIQEGSGTYSLLFYEHVRSSGVVPQIS
                     CILGTCAGGGCYSPALTDFIFMVDKTSEMYITGPRVIKEVCHEDITGYELGGPKVHSQ
                     ISGVADFVSKSEQACLDDVKKLLSFLPSSCDEKPPIVETGDDPNRICEDLATIVPDAP
                     RAPFDMKRVIRSIADQGDFFEVKPRFARNMITGFLRIGGYPIGVVANQSMVMAGAIDS
                     DASDKAGRFYRFCDCFNIPILTIADVPGYLPGVKEEYKGIIRHGAKMLYGYIEAEVPK
                     LLVVVRKAYGGSWAAMGSKTMGADFSYAWPTAEMAIMGAEGAVEILYRKELQAAEDRS
                     AVKQEKIEEYRNKFNSPYLYGGKMVIDFLIRPQDTRAHLYRALKLLWNKPQEKIPRKH
                     GNVPL"
     gene            complement(17327..18424)
                     /locus_tag="PLT63_02290"
     CDS             complement(17327..18424)
                     /locus_tag="PLT63_02290"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOD97578.1"
                     /translation="MAEKKRYGMLEMDDEQKKHFDKLIVKKNFTKLDPELNAMIMGAR
                     KGMPPIIDIHTHPYTKTGWRSLGKFRVHLEKYLYGKKDITPEQISASLPSEEEWVKTF
                     QELGVKVLPCGWAAQTTMGDDPLYADNTNDEIAAMRDKWPDTVISGWGSVDPWQGYKA
                     IKEAERCCKELKLVGLKFQQVGQAFHVNDRQFYPLWDACQELGMPIQLHTGFTGLGSG
                     APGALGTKIKYTMQLIPDIDDISADFPNLKIIMLHCSDGRDEDAVLLCRHKGNIFREL
                     SGIWPNYLPENAPKTVYEANRRQKDKYMFGSEFNLFPLDGILYQHMQFGWREGVLESM
                     FWKNTVRILGPELERAGVDLKQWKVGNLEEV"
     gene            complement(18633..18848)
                     /locus_tag="PLT63_02295"
     CDS             complement(18633..18848)
                     /locus_tag="PLT63_02295"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HOD97579.1"
                     /translation="MSEEILAPMAGKIIKILVKVGDKVAEDDILLTLDAMKMENPMVA
                     PVDGVVKEIRVKENDEVEPDDVMIVLD"
     gene            complement(18930..19928)
                     /locus_tag="PLT63_02300"
     CDS             complement(18930..19928)
                     /locus_tag="PLT63_02300"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOD97580.1"
                     /translation="MADSKAILVVGETEKGKMASITRELLGGARVLADQSGGDVCLLL
                     LGEIAAALASEGISLGADKVYVATNAAYNEFSSDAYTELVSGLCAKIAPALCLMGQTD
                     LGCEVAPRVAARLGAALAMDCVEVVFDAENGRFQQTRPAFGGKALSVLASTKGLLQIN
                     TVRAKSMQAAALQDGRKGETVTIAEAPAPGVIKVKLLEAKKTEAEGIKLEDAKVIVSG
                     GGGLGGTEGFAMIKELADLLGGVVGATRVPVDENWVPLSMEIGQTGKIVSPELYIAIG
                     ISGAAQHVTGCISSKKIVTINRDPDANMFKISDLGLVADYKAVVPILIEKLKAMNT"
     gene            complement(19941..20738)
                     /locus_tag="PLT63_02305"
     CDS             complement(19941..20738)
                     /locus_tag="PLT63_02305"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOD97581.1"
                     /translation="MDIIVCIKPVLDPELPPLKFAVDGKKNQVIPPEGMPFLMSPYDA
                     LAVEAALRVKEAKGGKVTVLTLGTKYTEEVMRKAIAMGADEGFILTDPAFEDSDGFAT
                     AYILTQAIKKIGSFDMILCGRQAADWDVGMVGSAMAEYLGIPVVIRAKSIEVLDGKIK
                     VERVVMNGSEVFEVALPALVTMSNEMGQARIPAGRGIIMAAKKQFQKWGAADLGAEAS
                     KTGAAAARNNLIKLFIPSYQRTCEYVTGKDPAEAAALLAEKILAIKP"
     gene            complement(20749..21021)
                     /locus_tag="PLT63_02310"
     CDS             complement(20749..21021)
                     /locus_tag="PLT63_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97582.1"
                     /translation="MKIEEKLARNKFDIDREVHIQVDEEKCKACESKVCLYACPAECY
                     VLRAEGHVTFSYEGCLECGSCRFSCDKGTIAWVNPRGGFGVCYQYG"
     gene            complement(21018..22304)
                     /locus_tag="PLT63_02315"
     CDS             complement(21018..22304)
                     /locus_tag="PLT63_02315"
                     /inference="COORDINATES: protein motif:HMM:NF013314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOD97583.1"
                     /translation="MDIFDAIIVGAGPAGCAAAYTMAKAGLNILVLERGQYVGAKNMW
                     GGAFFGPVMNELFPGFWKEAPVERFLKRHVISFMTKEDCLSIDFKSSPGDFDEAPGFV
                     IMRAKFDRWMGSKVEEAGAIIATGLEVTDFIFEGNRITGVKVGDEDFLANVVVLADGV
                     NSLLATKAKLRGDFKAVDLKQGVKEVIRLPREVIEDRFGVSGNEGVVMEFVGSCTHGL
                     PGGGFIYTNQESLSVGVVVQLSALLENKVKASDLIDEFKEHPAVRPLWRNGELLEYSA
                     HLIPVSGINMMPKLYADGILVAGDSAAMVLGTGLILEGANFAMAAGIAAGETVIKAKE
                     KGDFTSSSLNDYEQRLKSNFVIKDLETFRRAPHFLENPRIYNAYPELACGLMGSIFHS
                     DGKPRRNTLEVVMESMKGKVSLWQIAADAMKARKAL"
     gene            complement(22342..23505)
                     /locus_tag="PLT63_02320"
     CDS             complement(22342..23505)
                     /locus_tag="PLT63_02320"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOD97584.1"
                     /translation="MDFELTTEQTMFKDMAKEFAIREVQPYVKQWDRDCRTPPEIYQK
                     MGELGFLGINAPEEYGGLALGWMTVGIVTEEMAYYDLQLAMSMFSRVSLGMLPLIQSG
                     SDEQRQKYLPGLIQGIKTHCFGTVEPNAGSDATAIETTAVVDGDDYVINGNKTWITNA
                     CHSDFAMVVCQTDKTKGTRGLVVMCVDWGTPGFTRAPIENKMAFRHHDHGQLFFRDCR
                     VPKDSQLGGFGIHYGMRAAFSNVEHTRYGIACAAAGAIKACLDLTVNYCKDRIQFKRP
                     IASFQMVQDKIADMVIAHEASQLLAYKLGFQKDKKVDVTKMSSVAKYHNMLMLKKCAS
                     DAVELHGAYGLSDEYPVERIYRDCMAPYIYGGTAHVHKLVLGRLETGISAVAK"
     gene            complement(23870..25270)
                     /locus_tag="PLT63_02325"
     CDS             complement(23870..25270)
                     /locus_tag="PLT63_02325"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOD97585.1"
                     /translation="MKEDSKPKWLSYISDTLLDAFVDNHYECPIVIDKDGIVRFMSRH
                     NVGLYGMTPEQAIGKHIVEVNKNSGMHETLITGKVEIGATYQLGGRQQIVARIPLRDE
                     NNKIIGVLGKRIFNKVDTIKDLYKKVNILEGQLKYYRKEATNQNIYAYDKLVAGKSQL
                     IQETMNNALQAAKSDATVLVTGESGTGKEACAIFIHKASKRTDGSFIKVNCAAIPAEL
                     IESELFGYEGGAFTGAKQGGKPGKFELAHKGTILLDEIGDMPLNMQAKLLRVLQDKEI
                     ERVGGTKPMKVDFRLIASTNKDLQEMIKKGTFRTDLFYRLNIFTINTPSLRSIPEDIP
                     EIASFLMNELSQKDNMPAKLITDAAMIALQRYPWPGNVRELRNVIERALIITEGPEIQ
                     LQHLPTRIKELGKKSSNGNRKYIGPLRDIVAETERKVIVDAIRSVEGNKQRAAKILGV
                     HRTVLYQKIKRYNIPL"
     gene            25453..27573
                     /locus_tag="PLT63_02330"
     CDS             25453..27573
                     /locus_tag="PLT63_02330"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOD97586.1"
                     /translation="MENGHDLQRLFYPRRVTIVGAGDNPLLINGRSLMFMLRHKSTAR
                     IYPVNPNRDTVQGIPCYRRIADLPEVPDVAVIVVGKKLVPAAIEELGQLGCSFAIINT
                     SGYAETGPEGLAEQEELLRRSRRYGMRLIGPNCLGLINLISPVILSWCATLEREQGDL
                     LPGDVALISQSGAMLGSIWDRAMGLGLGYSYLLSSGNEADLGMADFMEYFAQDKNTKV
                     ITGFVEALRNPEKFLQGVDQAHSNGKAVVLYKVGRTAEGRRAAVSHTGSLTGSDETFD
                     ALCRAHGIVRVDRLDALITSALALRALPPARGGRLGIFCCSGGAAGLAADQMRGTGLS
                     IAPVGPAFEQDITNITNFHPPHNPLDIIKGPLKSFDVITEAMKRFVREDNFDQIIILM
                     TTMYLQKIAPKLMLTGLKDGPEKPVLACWLGNKVVAKPSEEMRNGGVITYHDVESCLE
                     AARALAVIGKHRRRLAMELSYKQPPRDAKSGALKIIEQCEGRLDEASSKAILALYGFS
                     TPRGKLVRNLDDAIATALALGFPVVAKGISPDVLHKTEAKAVCLGISSEKALKKAFET
                     VATAIESRGTLRGVLIEEMLSEPVAEVIVGCSLDEPCFHKIIFGLGGIWVEAMKDISV
                     RMAPILEEDAEEMVAEIRGANILDGLRGKPSADKGALVSALVSLSALITDLREEIGEI
                     DVNPLMVYQEGAVVADALLSCRRS"
     gene            complement(27592..28170)
                     /locus_tag="PLT63_02335"
     CDS             complement(27592..28170)
                     /locus_tag="PLT63_02335"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HOD97587.1"
                     /translation="MNPCIIVIDMLEDSFSEGHRNPVTPFAKAIVPNINRLTDIARSK
                     NIPVIFSMDSFMRGDFIFKGKMREHSIRGTKGAELYHGLSHTEEDIYSYKRRFSTFFK
                     TDVDQTLRLFGVDTVVLTGINSHWCVLSSAFDALSNDFRTYILEDCSASYSEEIHRAA
                     MNMYRSNPLYPLFKIMTLEEFLKELNETQAAR"
     gene            complement(28167..28640)
                     /locus_tag="PLT63_02340"
     CDS             complement(28167..28640)
                     /locus_tag="PLT63_02340"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOD97588.1"
                     /translation="MKTLELAFEKIRDIINESPFYRHLGIKLVELSARGSEVAMEIRE
                     SLKSVYGRAHGGAIASLADSACSLSLVTVIKENETFATQSLEVYYLAQVEGGMLKAKG
                     WVVHKGRHSAVLEADIFDEAGRKVAHAHTVHVIRALRGEEGDTFIDKIRKSGGIK"
     gene            complement(28984..29808)
                     /locus_tag="PLT63_02345"
     CDS             complement(28984..29808)
                     /locus_tag="PLT63_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOD97589.1"
                     /translation="MSDKVETRNVKNNASKKGNKDLLFLKSDGIATIRLNRPGKKNSF
                     TMDMIDQWVEALEKSRKDADVKVIVVTGTGDSFCSGADLAGLRDAENCAIDHKNMLWE
                     QIHRVALIMEDIDKPIIAAVNGIAVGAGMDMALMCDLRFVADTARFSEGYIRIGVVPG
                     DGGCYFLPRLVGVAKALELFWTGDFIDAREALRIGMVNRVYPADKLMEETYDFARKIA
                     DSPELAIRTTKRATYQSSRVELRTALDLISSHVGVIRTLKDHHEAVNKMMAKLSSK"
     gene            30337..31683
                     /locus_tag="PLT63_02350"
     CDS             30337..31683
                     /locus_tag="PLT63_02350"
                     /EC_number="7.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF015910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOD97590.1"
                     /translation="MLFGLMETGFPLFYWGNALMIVIALILIYLAIAKDMEPLLLVPI
                     GFGMILVNLPLGGMMDYEMRLKAPQAGTVMEISTQEGKGFDKGSVICKLDSGEVVAPV
                     FGRVGELIVEKGQHVEKGQVVASIFTMEQTDQAVYPSRPVGLLSRIFQYGVMWQILPP
                     LMFLCLGALTDFGPMLANPKTLLLGAAAQFGVYLAFFMALFVGFTLPEAASIGIIGGA
                     EGPTTIYVSSVLAPHIIGATAVACYSYMALVPIIIPPVIRLLTTKKERGIFMKPSLRK
                     VSKVEKIVFPLITAIIVILIVPTSAALMGCFMVGNLFKECGVTERLRKTAQTTFVDLI
                     TIFLTLAIGASMPAQNFLQPKTLLILVLGVISFASAAGAGVLLAKFMNLFLKVKINPM
                     IGGAGVSAVPMSARVVQVMGQKENPKNILLMHAMGPNVAGAIGAAIAGGVFIGILG"
     gene            complement(32227..35733)
                     /locus_tag="PLT63_02355"
     CDS             complement(32227..35733)
                     /locus_tag="PLT63_02355"
                     /inference="COORDINATES: protein motif:HMM:NF024904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOD97591.1"
                     /translation="MRIMTLHLKAYGPFTDARLDFAGDLPGLHVVHGANEAGKSSALR
                     ALTDFLYGIPERTRDGFLHGNDQLRIGGTLRLADGREGSFVRRKGRKNTLLTPDEGGA
                     VDDRELAPFLSGVSREMFATMFGIDHEELVRGGNELLTQRGRVGEALLAAAIGSEKFR
                     ALLKELEEAAAELFLPRASKPRINAGLAEFKALRDRARRESLPAREWDRHWTRRRELV
                     AEVRRIDGEIGARQGERQRLLRIGRVLKGLAERRELIARLAAMGEVHILAADFPSRRA
                     AVLRDWEHAREAVARAGDRLANLRAVCAQLPPPSSLLAEAVLVDELQQDLGSHLKALR
                     DRPDLEGRLRQMRNDARRLMETIGIEPRLESVGEIIPQVERNRALVTELARRHEAVLT
                     SLKKAEKTLERRRRDQEETERELRGLPDGGDPAALREAVRVARKEGDPDGELFKARKA
                     LQEAEGKAQRTLAGLGRWRGDMEAALILSVPARESIERCEGEWRRLEERRREIERAAG
                     ENAAETLAVRKKLTGFALAGMVPTEQDLEVARRRREEGWRLIRGLLAGDARNEDGERR
                     FHADLPPPEAYEDAVTAADDIADRLRREAQRVHVLADLRAADDSLAERRRQLERETAE
                     LVAQEKKRDDDWHALWAPGGIEPLSPREMAAWLSSFHALREEILRAQAAREELLALEA
                     RHASTCASLREALRPLRPEPHRKSELFRDLREEAEHHLEVRTERNRRRGEMRRQMDRQ
                     AGQVREAAGEKETARAELRDWQERWRAAMTGLGLSLDTPPAPALSVLDQYTALFGQTK
                     QIADMDRRIWAINEEARRFTEKVAEFVRRRVPEYAACPPEEVVRRLKQEVDGARLREK
                     ERQNGAKRVREAEEELRAAATDFRVKEQRVADLLKEARCRETSEFEAAEARSVACQGC
                     RERLAAVERFLLENGDGLPVTVLEAEAAAVSPDELPAALTGVNGRLEELQSRRQELNT
                     EIGEENAELRRMDGSSAAAEAEEQAQAALASLRGDVARYTRIRAAFHLLKNAMNRYRE
                     KNQAPLLLRAGELFGRITAGAFPRLMTDIGPEDEPVLMGVRSDGARLGVEGMSSGTRD
                     QLYLALRLATAETYFQGEPLPFIVDDVLVNFDEERTAATLRVLREMGRHTQVILFTHH
                     RHLAALAEELAQAGAGVAVRKL"
     gene            complement(35730..36992)
                     /locus_tag="PLT63_02360"
     CDS             complement(35730..36992)
                     /locus_tag="PLT63_02360"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HOD97592.1"
                     /translation="MIRFIHAADVHLDSPLVGLEQYGDTPVEEIRGATRRACENLVAL
                     AIKENASFVILAGDLYDGDWKDYNTGIFLARQMARLKGAGIRVFIAAGNHDAASQITK
                     SLQLPDNVYVFSHRKAESIPLPELNVVLHGRGFAERIVSDDVTRKFPPARPGMFNIGV
                     LHTSLTGREGHDVYAPCTPDGLRGRGYQYWALGHAHRHEVVARDPWIVYPGSLQGRHI
                     RETGAKGCVVVRVEDGEVREVRHEPVDILRWSPVEVDATGTAGPREVIERAQRALAQE
                     WDRHGGLPVIARLLIAGETPAHASFRAHPEHWEAEFRAAAQEQGHGRLWLEKIRFDTR
                     PPAAAAGWEGDEAVAAILKELRLLAADPMQATGLIEEISQLHQKLPPDLLPGPGGGDP
                     GDARRLADILAEARDLLESRLLRGGGPA"
     gene            37277..38665
                     /gene="pcnB"
                     /locus_tag="PLT63_02365"
     CDS             37277..38665
                     /gene="pcnB"
                     /locus_tag="PLT63_02365"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004652 - polynucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HOD97593.1"
                     /translation="MAPRIIPRKEHNISRKQINPYALRTLYRLRDEGFIAYLVGGCVR
                     DLLLKRTPKDFDIATNATPGQIKRLFRNCRLIGRRFRLAHLHFKEEILEVSTFRADGP
                     AEDSEEEGRPGTGEGKGAVAGHPRHLKDDNGVVLRDNVFGTPEEDALRRDFTINALAY
                     NIADFTVIDYSTGLRDLRDRIIRPIGDPAVRFTEDPVRMLRAVRFAASHDFALEPAAW
                     EVIRRLAPTISRSSPARLYEEVQKLFLLGSAGRVLGLLEESSLLAALLPGVDRRLADD
                     EVHRRRLRANLDYLDQRRQSEMPPTPSFLLAALFGPSLEEAALAGHREGIPFQQALDS
                     ACGAALMELGNTVTVPARIGDHLRRILAMQQVLRRRPPRRPTALSGRQEFAEALEYLR
                     MTTAVQREGQAAREWWDAFLAGVPPEKITGTADADTPAPEADARKKRSRRRRPRRRKA
                     AVPAAASADATG"
BASE COUNT         8332 a        11350 c         9584 g         9451 t
ORIGIN      
        1 taaattcctt cagtaactcc tctttttcaa gcttcatgaa tatcttcctc ctaagatatg
       61 taaaacatta ccctttgcat taattgtccc acccgttcca ttgtttgggg tgctttatcc
      121 gggcggacaa gcgttttact taaacatccc ttccaagcag tatcgatcaa ggacctcttt
      181 tatgatcgac tctttagtgc tgcccccgta aaaggtcgca aatgagactg gacgcgacat
      241 aacggtaatc accttgtaga attgattgct tctgacgttc atatatgaaa atgcccggcg
      301 ggtccgtcat caatttcatg agattgtata tatgcgcaag ctgggcgcgc caatcagacg
      361 gcaggctttt gttccggata attttgtcaa gaagacaggg aaaacggatt tcttcatgga
      421 tttatgaatt gcctcttaag aagtctgcat agttgtgccg tccgcctctg gctcgagaga
      481 cctgctactt tcctatcggg acaattcgat gattgttttc ggcgtggcag ggcgatcgcc
      541 gccaatgcgg gtgatacagg gacaacgcac tcaccgccag ctcacggacg aaacgccacc
      601 ttctcaagca ccacgcaacc agtattttga atatagaccc gatccttcca ccgaattgtc
      661 cgttgtctca gcagcccaac attttttaag ggaggttaaa ggatcaggcc caaggagggg
      721 ttgaacccaa tcctttaacc tcttacagca tcagttagaa cggcccgtca cctaaatatg
      781 acggcatcgt aaaaagtcaa ataatgtccc atttgggacg gcatcgtaaa aagatccgca
      841 ggcgaggcgc gcgaatcccg aggaatgagg cgtacttgga cgtacgtcgc agtgacgagg
      901 gatgaagcgc tttcgaagca tccggacttt ttacgatgcc gtcaaatatc attttataaa
      961 agggctggag ttatggctcc tccagcccca gaagtagtgg aatccaatat ggtgcttctg
     1021 gtccacttaa ggagggttaa agatactgac tggaggtagt taggtcagta tctttaaccc
     1081 tcttcccaac agcggtgaac tgtatacgcc acaaaacagc gacatttata gctccggcat
     1141 cagcgacatg agcaagaaca atacccatca gatcgtgtgg cttcctgacc tgatcatagc
     1201 tattggttat tgtttcttct cctttggagg ccgttccagt cccttcacga tctgcgaccg
     1261 aatcatccgc ggcagcagca tgtgatgctg ggggcagatg gatttcggat tctggtatac
     1321 ggcgttcgca aaggcaatca tgtatttccg aatgtcttcg taacgcacca cctcgtcgac
     1381 taaacccttt ttcgcacagt agaagggccg tgatttctcc tcgtaatgct ttacgagatc
     1441 attcattttc tggatagtgc catcaaggga cctaccggca tccttttcct tcacgagccg
     1501 acgggcgtaa ctggccgctg ccgccgtctc tccgtgcata acatttattt ccgtcgccgg
     1561 cgtccccagg gtaaaggcat tgttattgtt ggccgtcggg cctcccagaa cataatgcgc
     1621 cgccgccgtc cccttccgga ccacgaccag catcataggc agttcggttg actcgataga
     1681 ataaatgagc gcctgcccaa gcccaaggag ctcggctttc tcggcaatat ctcccacgtc
     1741 aatacccgat gtatcctgga accagatcat cggcacccga tcgcggccgc agagggtcac
     1801 gaattcattc atcttgataa gtccctgccg gtaaagtttc ccgccaatgg ctccgtactc
     1861 gttcgtatac tcgggatatt tgggtatcaa tccctggcgg ttgccgatga cgcccatgag
     1921 gaatccatcg accttcacca agccacagta catctccggg ccaaatccag gctggaattc
     1981 catgtgttct gaattgtccg tcagtcgcgc cagcatctca tcaaaggaat agacttccct
     2041 ggtattcaca ggaatgatgg attccaaatc cgctgctgat aatttcggtt ccgctggctc
     2101 ggccacccgg aaaaatttcg ggttgtaggc cggcaggtag ttcatccact ctttgatacc
     2161 atcgagaaca tccgtttccg tttcatagac ggccttgaaa aaaccggtct cattataatg
     2221 gatggcgacg cttcccggag gtacggattt gaaatgccgg gttgcttcga tcaatgtttc
     2281 cgctccttca acatcaaagg aacccttggg actcataccg ctcacgatgc cgccgccacc
     2341 aaccgctatg ttacatttct tatgggccag caaaatcgtc gggctgattc cctgataacc
     2401 acctcccgcg gggttggtac cgtaaatgcc cgcaatcacg gggacacctt cctgctcaag
     2461 ttccgcatga cggaagaagc aggttccttg gccgcgacgg tttgcgtaca ccttctcctg
     2521 ctcgggaagc tttacgccgg agcagttgac cagccagacc agcggcaaat ggaggatctt
     2581 ggccatgtcg gtgacccgca gattattgtc tgcctgcccg gcgatccatg cgcctgcgat
     2641 ccatttgtta ttgaagccga cgaggacaca ccatttaccg ttaatcctgg ccagaccatc
     2701 aacgacattt gtctgtccag actcctcatc gtcagggtca tagatggtat gcaggggggt
     2761 gaaggttccc ggatcaacca gatattcgat tctttcccag acggtcatct ctccgcgctg
     2821 gtgcaacgtt tccgtggaaa cgccgaagtt ctttatccga tcaacttccc ggtcgatttc
     2881 ggccatgatc tctttaatct tggcgacatt gcctgtcatc atctctttga aggcaggctt
     2941 taggggctta cccagattat ccatctgttt aaaatattgt ctcattgatt ttctcgctcc
     3001 ttcttacggt tgaccgcgct tttgcgcgcg attgacctac tttttagcct tcttcgctcc
     3061 cagttgatcc aaagccacaa caaatttgca gacattggcg gacccttcca ccatggaata
     3121 ggtaggagca tctcggtaat agcgtgcaac gggatattcc gtcgagtaac cataggcccc
     3181 tagaatgcgc atggcataat tggagcattt ggtcgccagt tctcccgcca ggtatttggc
     3241 ctgcgccact tccagggtat tattcagatt accctggtcc ttctgccaag ccgctttata
     3301 gacgacaagc ctcgcggcct caatctcgca taccatctgg gcgatcatat cctggatcat
     3361 ctgatactgg ccgatcggct tgccgaactg ctcgcgctca ttggcatact tgagaacgct
     3421 gtcgagacag gcctgtgcca aaccgacccc acccgctgct gcggaaaggc gggtttgatt
     3481 gagcgatccg aagacgatct tggcgccgtc gccgggattc cccaggatgt tctccttcgg
     3541 aacctttgtg ccgtcgaggt atatctcgcc ggttggtgag gagcggctgc ccagcttgtc
     3601 caagtcggtc gttctgatcc cgttgaaatt cttcagctca acgacaaagg cggaaaggcc
     3661 cttgctgccc ttcgaacggt ccgtataggc ataatagatg atcgagtcgg cgacgctggc
     3721 attggaaatc caggttttgg agccgttaag cagccaatga tcccctttgt cttccgccat
     3781 cgacttcatg gacatgacgt ccgatcccgc gttgaattcc gtaatggcaa aaccgcccat
     3841 gtactcggcg ttaaccagtt tctgaatgta cttctttttc gccgcctccg tcccgtaacg
     3901 gaaaatcgtg aaggcacagc ccagagcttg catgttaacc tgcactctga gcgagctgga
     3961 tgcccgggca atctcttccg taaggatcat tgccgccagc catcccatat tatttccacc
     4021 gtattcctcc gggatgacgg tcccaaaaaa tcccagttcg gccatgggtt tgacggcttc
     4081 ctcgtaaggg aaataatgct tctcatccca ttcgtcagcg aagggggcaa tcttttcggc
     4141 cgcaaaatcc ctgaccattc cacgtaacat ttccagttct tccggtaatg caaaattcat
     4201 tgacacttct ctccttattc aattaaattg acattgttcg actactcaga tttttttaat
     4261 ctctccgtct tccaatctcc cgacttcaga tggacattag cccttctgaa aaacatagtt
     4321 catacggtca ttcggtagtg ggtgcacaac cgccttcatt gccatcccga cggaaagctc
     4381 actgtcgggt atcgaagaat ctaccctgcc gaagaccttg taatctccgt aatcgagcac
     4441 cgcgatcgta tagggaagat cctgggtgaa acctgtcggc gcgaacgtga gggtgctgaa
     4501 gctgagcagc tttcccgttc cactcacctc gaaccattcc atgtcgctat ccaacgactt
     4561 gtagcagtcg gctctgggag gaaaaaattt caaaccgcac ttcttgcact tggtccccat
     4621 caatttgccc tgctccagat agccgatgaa atcgttaaca cgggtcacgg ctgtgaagct
     4681 taccgtaccg aatttactga accgattatc catttctttc tttgccatat tattctctcc
     4741 ctaagatcgt gacattagca tagagtccga caccacccac attgtggacc agacctatgg
     4801 ctggatcctt gacctgaata tcccccgctt ctcctcttaa ttgcaggact atcgttctca
     4861 cttgagaacc tcccgtcgcg ccgacgggat gtcccttgga aagcaggccg ccatccacat
     4921 tcaccggtat tcgcccttcc ttgtacgtct ccttggcacg gatcagggcc ggcccttcac
     4981 ccggcttggc aaacccgagc gcttcatagg ccaggatttc ggcaatggtg aaacagtcgt
     5041 gcacctccgc cacgttgata tcaccgggct tcatgccggc cataccatag gcctgtctcg
     5101 ccgcctccgc cgccgcggga aggctgtaga acgtttcgcg acccgccata ttgacggcgg
     5161 ccgtagccga accgagaccc tttatccata ccggtttttt gctgaaggcc ttcgcttttt
     5221 cctcattcgc cacgatgacg caggaacttc catccgcgtt attgcagcag tctcccaccc
     5281 tcaacggggt ggaaaggatt ccggccaggg gggccggggc tttgaaggca tccatatcca
     5341 acactttgcg attcatggct ctgtcgttga gaataccgta cgtggaagcc ttgatcctca
     5401 cgagggccag atcttccggg gtggttccgt actgtgccat atggccctta gcaaacatgg
     5461 cataataagc aggcatcatc gtaccgaagg gggcctccca ctgaatatcg gtaccacgcc
     5521 ccattcgttc ctgcgattcc gcggcgctga tttcagacat cttctgaaaa ccgagaacca
     5581 ttaccatgtc atggagaccc gattttacga gggaatacgc caggcgaagc ccggttgtgc
     5641 tcgacgagca aagcgattcc acgtaaaaag tgggctgcgg gtttagtccc agatactcag
     5701 cgaagacacc cgatggcgac ctctgtttgt cgtattccgg ggcggaacag acgatggttg
     5761 cgtcgatatc ctttgtcgtg accttcgcat cctccatcgc ttccttaaaa ccttcaaaaa
     5821 ccagttcccg gactgacccc ggataggcac gaacaaattt actttgtcca acccctatga
     5881 ttccaacttt actcatttat ctcctcctat atatatttga taaacttatt tgcccttata
     5941 gaaaggtttt ctcttctcaa gaaaagccct cataccttcc gttttatcct cgctcgcaaa
     6001 ggccagtgaa aagcaggtgt tctctattgc tcggccggtg gcaagatccg caccgacgga
     6061 attctgaacg gcatacttcg ccaatcccaa ggctaccggg ctgtgacgag atatcacctg
     6121 cgccatcttc tttgcttcag ccatcagttg ctcgtcggga accacctcgt tgacgagatt
     6181 gatgcgcaag gcctccgccg cgctgatcat cctccccgta aggacaagct ccatagcctt
     6241 accgagtccg accaaccgcg taagacgaac cgttcctccg cctcccggaa tgatgccgat
     6301 attgatctcg gcctgtccga acttcgcact ttcagcggcg atacgcacat cacaggccat
     6361 tgccagttca cagcccccgc ccaggcagaa cccggcgata gcggcgataa tcggctttcg
     6421 ggactgttcc atcctgaatg tcagatccca tagcgaagct ctactgaatt gctccaccgc
     6481 cgtcttttcc accatggcgg cgatatcagc cccggcggca aagacttttg gacccccggt
     6541 gatcaggata gcccggacat ccgtatccgc ttcgagttcc agaaaacacc gcgacaaatc
     6601 ttctctcatg gccgggttga ttgcattcct gacctcgggg cgattcagcg tgataatccc
     6661 caacttttcc tcgacgctct tttcaaaaac aatagtagag taactcatcg ccttttacca
     6721 aatcggctaa aattcgacat cggtgcgggg tttcttctct cccgatgaat agtcgtacca
     6781 tcccttcttg gttttacgtc ccagatcgcc gctctcggca agctgctcca ggttcatgtc
     6841 gggccaccat ttaggatccc ggtagctgtc gtacaaaccc ttgaggacgc tgagaccgat
     6901 atcgagacca accagatcgg ccacttcaaa gggccccatg gggtgattaa gagccaactt
     6961 tgccgccttg tcgatgtcct cgatgctggc aacaccttca ctgagacaat gataggcctc
     7021 gtttctgaag gcctggttga tcctgttgac gaggaatccg ggaacatcct tcacccgcac
     7081 tgtttctttc ttcaaggagt gaataaattt ctcggccgtc tcaatagtat ccagcgaggt
     7141 cttcagacct ctgattattt ccaccagtct catggcggga acggggttaa agaaatgcgt
     7201 gccgatgaat ttgtccggac gactaacgca tgttgccagt ttcgtaatgg ccagcgaaga
     7261 ggtgttggag gcaaaaatcg tatcggggcc gcatatcttg tccaatttct cgaaggtctc
     7321 ttttttcgac gcaaaatctt ccgcaatgga ttcaataaca aaatcgacat ctttggcatc
     7381 ttcccagtcc gtggtcagct tgattcgacc cagaaccgcc tgcttgtctt ccgcggtaat
     7441 tttttccttt ttgaccaaga aatcgagacc tttattgatc aaattcatcc cccgctccat
     7501 atttctgtgg agcttcataa ccacatcgta acctgactgc gccgcaacct gaacaatgcc
     7561 ggcacccata gtgccggacc ctacaaccat gaccttcttt acttccatct ttctcctcct
     7621 acgtgattat tccaaatttg gttttgatcc tctggcaaac gggccctttc gattagatgc
     7681 acccccatct caccccgtga tgatgcgccc ggatcagcct atcgtcagtg ccgcttcggg
     7741 acattccatg accgttttac cggctttctt gatgatatcc gtattcatat aggcctgcgg
     7801 caggacctgc tcggcataat accgcgcgga agcaatcttg ccttcataaa acgttcgatc
     7861 gtagtgatta tccggaagct cagagagcct ccgtttagct atcaaggcct gctccatcag
     7921 acattcggca acctgcacct gcgcgcagac aaacaatgcc tttatggcat aaagcggaat
     7981 caaactcttt ttcgtttcaa agtcggaata ccaggaatcg taaatgcttt ttatctcttc
     8041 gaggcaatcg tatcccttct ttagattcat catctcatca tgaaagccat cggcgccgag
     8101 gttcgcctcg atgaatcctt tcatcgtcga cagccaaagg gaaaaaggct tcccgtcttt
     8161 catcctcatc ttacgcccga ccagatcgtt gccgtgcatg aacgatgtgc cttcccaaat
     8221 ggtcaaaatc ttcgaatccc gcaggtactg ctcaaccgga tattcactcg tgtatcccac
     8281 tcccccgaag atctgtatcg cctcggcgac gagatttaaa ctcgtctcgc ttccatagca
     8341 tttcaccagc ggcgtaatga tctcggccag ttcaccgcac atcttcgccc tttcgacgtc
     8401 cttcgagttg gcttggatat caaggtagta aaaacccttg aagatcatcg cccggatgcc
     8461 ttccgtgtgg gcttttaaat ccaacagcat tctccggaca tcttcatgct tgatgatcgg
     8521 tacgcgctcc gcgttcttct cgccaaaagg acgaccctga atgcgctcaa cagcatactg
     8581 agcagcgtaa aaataggctg ccgtggcctg acagttcgcg ttatggcccg ttccgattct
     8641 cgattcgttc atcatgtgaa acatcatggc cagaccgcgg gaagcgccgt tcttgtccgg
     8701 cggcggacct accctgatac cgtaacacgc gtcgttttca ccgaagctca aaagggccgt
     8761 ggcctgcgct ttaagaccca ttttgtgttc aatccccatc gtggtcacgt cattggcctt
     8821 acccagggtt ccgtccttct caacccaaat ccgggggaca atgtaaagtc ccagccccgg
     8881 cgaccctgac atcccccctt ccggacgggc caacaccata tggatcgtgt tttcgcatat
     8941 gccggaatcg ccggccgtga tgaacatctt cgtgcccttg attttatata tcatgggatc
     9001 atcagtggga atggccctgg tgatcgcatc tcccacatcc gagcctgcgc tcggctcggt
     9061 caggcacatg gtaccctgcc aatcgccgct gagcatcttg ggaataaaca gggcacgatc
     9121 ttccttcgta ccgaagcgga tgatcagatt ggccgcgccg ctcgttaatt taacgtacga
     9181 cgtcagcgcc ggacaggcgg aggtattcat ctcatgaacg gccttgtaga tgatgagcgg
     9241 catgccccct tccagttcga tgcattcgct gctcgatccc cagccgtttt gttgaataaa
     9301 acgatatgcg tcgctgtacc ccggcgcggg tgtgactttg ccgtctacga atttcgcgcc
     9361 gacggcatcc ccttttgcat taatcgggct cacaatttca caggccacct tgtaagcctc
     9421 gttcaacagg atatcgatgt catcaaggct gaagttttct ttaaagcgat cgcattccaa
     9481 aacctcttgt gaaggcagcc attccttcaa aatgaacttc agatccttca tatcgtactt
     9541 gaattccatg gtttgcttaa cgtctcctct gcgaaagaag gaacataaac taatgtttct
     9601 tcccgcttat gcgatataag ttataaatat ttataatact cgttaacgag attattttcg
     9661 gtttctgttt tgaagtgggg cgggcttttc acccgcccct tttatttaat attcaaatcc
     9721 tttattgggg atttttcatc ttttgggcga tgctgtctct caggacgtat ttgagcacct
     9781 ttcccgttgg cgttctcgga agctgcgtga tggtttcaat tcgctccggc catttgtatt
     9841 tggcaactcc ttcctggttc atgaaggctt gcacttcttc aagagttatc gtttcgttcg
     9901 ccttcagctc aatgaaagcg caacactttt cgcccagttt cgcgtccggc atccccacca
     9961 cggcaacatt ctgtattttc ggatgcttgt acagaatgaa ctccacgtcc tcggcgctga
    10021 tgttcattcc tccccgaatg atgatgtcct tgaggcgggt ggagaaatga tagaacccat
    10081 cttcatcgag ataacaggcg tcgccggtat ggtagaagcc gtctttatcc catttattgg
    10141 cattgagatc cggccgcttg taatacccgg caatgacaaa aggcgccttc tggcaaaatt
    10201 cacccacctt gccagcaccg aggatattac cgttttcgtc gacgattttt gtctgactgt
    10261 aaagctgcgg caaaccaatg gttgtggata cgagttccgg ttccaaacca acctccgtca
    10321 cattgagatg gccttcgttg gaaccgaacc cgttccagac gaggcatccg aatttcgcca
    10381 tcatctgctt gatgacggtc gtcggacaag gggcgccgcc gctggcgacc acccgcaacg
    10441 agcttagatc gtacttgtct agttcaggat aattcagcag cccgatgtgc atggccggaa
    10501 cgccggcata aagagtcgct ttttctttgg caatcgtctg ggccattgtc gcgggattaa
    10561 agccgtcgag caatatgagg gtgccaccca ccatgatcat ggggagaacg ccgacgctct
    10621 ggccaaaaag attgatccaa ggcagggcca tgacgacctt gtcatggacg ttttccgtca
    10681 ggtgatgcag aaaagctgat cgttcaaagg ctttccagtg attgtgtgtt cttgggcagc
    10741 ccttggggtc ggcttccgtt cccgaggtaa aacacatggt aagaacatcg ttaggatcaa
    10801 ttttgaaatt cttgtcaaga tagtcggcgg cgtatttttc ttcgagtctg ttctcccaca
    10861 acttctgaaa ggagaccgtg ccctccgggg cgtcatctcc gaggacgatc aggtgtttca
    10921 ggccggggtt ggtctcctgt aaacccttca tcatgttcac ataattgaac ttgccgtatt
    10981 ccttcgcaat aacgaccgcc ggcgaccccg tttggccgag gacatagttc aactcgtgct
    11041 cccgccattg catggccagg ggaacaacgc aggcgccgat ccgcgccagg gccatttccg
    11101 tgatgatagc ctcgacgcaa ttggggattt gcgtgacgac cagatcatcc cgcttgatcc
    11161 cgagttcgag aaagttcaag gccaacctgt ccatcatggt tttatattgc gaccaggtta
    11221 acctggttcg gttgtcgacc agggcctctt tgttcggaaa cgccgccgcc catcttttca
    11281 cgtaatcaca aaatgtccat ttatcccaga aacctagttg cgtgtagtat tcgatctgct
    11341 cttttgtaac attactctct attgccattt gctcttcccc tgattcattt tgatgtgttg
    11401 cgttgagcag tctcccggtt cagaaaccgg gagactgccg ctttcctttt ctctcaaccg
    11461 tgtctatgca aggtcaacga aatccttctt cgcccgctcc ttgatcagat agtgcagttt
    11521 ctttcccgcg ggattgaacg gcagttcctt gatatcaacg aacttgtaat atctcggccg
    11581 ctggtatctg gcgagtctcg ggctattttt acagaactct tcaagatcct tttccgtaac
    11641 attaggatcc ttcaaagcca cgtaggcgac aattgtttcg ccccattcct tgctgggcgc
    11701 tcctgttaca aatacatcgg cgacacccgg atggtccttc agcgcgtcct ctacaataac
    11761 cgggtggacg ttttcggcgc cggtgatgat catgtcgtct accctgccgc aaatggtaat
    11821 gtaaccatct ttatcccaga tggccgtgtc acccggaagc tgccaacccc ggtacaaaag
    11881 gcccttggct tcatcttccg gtctgttgat atagccataa cagccgtgca tggtttggag
    11941 ggcgatttcg ccttcagtct tgccgtcctt tgggcaatcg tcggctggat tgttggggtc
    12001 gccacggcgc ccgagatcga ttctgacgag tttgatcagg tcttcggtga tggcaaggcc
    12061 gatggttccc gccctttccg gtaattccca cggtttcagc atggtgaccc aatgctggtc
    12121 cgccgttccg tagccgttgt aaacacccgg acagaggatc ttcatgcacc tgaggcatga
    12181 ttccttgtcc agcggcgagc ccatggaaat gagcgcccgg aggctactga catctcgagg
    12241 ttgcttctcc tgggtctggc acattctctc atagataacg ggagcgctca ccatatgtga
    12301 gactttgtac ttttcgatgt aatcgagagc cagattgtca tcaaatgcct tcattatgac
    12361 gacggagccg ccgcagtgga gcaggcaaac ggggcccgtt gtattcccgc cccgatggaa
    12421 aagcggattc gtcgccaggc tgacgcactg agagtctatc ttatgcaatc cggcattcat
    12481 catgcagtcg aaaaagatac aggcttgact caatgtgaat cccttgggta atcccgtcgt
    12541 cccggaagtg taaagaagaa gtaccggatc aaacatcttg acatcctttt cagtcgccgg
    12601 cgccgtctta ggtttccctt cgatgaactt ctcataactc acatggcccg ccggaacttc
    12661 gcccgcaccc accatcacac agactcgggg tttggtcttg cataatgcaa cggcattcac
    12721 caccatattc ttcatgcctg cgtcatatac gaaaaccttc acctcactat catccatgag
    12781 tctggagacc tggccttcag gaagcatgaa attctgggag gcaaatatgc atcttgcctt
    12841 ggctgcgccc atgtacagag taaaccactc acagcagtta taaagatcgc ccatgacggt
    12901 atcaaaagtc gtacatcccg catcgagaag ggcatgggcg aactgatttg cctcttcatc
    12961 ccactgtctg aaagtcatgg ttcttttcaa tggatcgatg atcgccggtt tatccggcca
    13021 tttctgcaaa ttacgatata gttgggtagt gatggtataa ttcttttccc agagctcttt
    13081 taaaattgaa taatctactc ccttacccat ttcttttcct cctttttttt ttcgagaaat
    13141 gatttggtat ggattcactc cccgccatga aacaaaaaac atttgatcac cagttttgac
    13201 cttgaactac ctctaacacc tccctcctta tttatatttt gtgcgacgtc ttctcggact
    13261 cccaaacgaa actatttatt tctataagta taaggcaaat attctcttcc gaatagttcc
    13321 cgaagcgcga ccaatttcga aacctgggac gtcccatcgg caatttcgat ggccagaaca
    13381 tcccttagcc gctgctcaaa aggaaactcg tttgtgtacc ccggatgacc gtgcaggacg
    13441 aggcagtctc gaattgcgtt ggtggaaaat ctcggcgcca tccatttaac cattcccgcg
    13501 atcttcgtgc ttgattttcc ctgatcccgc atccaaagcc ccttataaca gagaagtcgc
    13561 acggcttcca ggatgctaat atgctctaca atgggaaacg aaacaccttc aaaggtagcg
    13621 atgggccggc cgaaagtatt gcgctgttta gcatactcta tcgtctcttc aagggaaacc
    13681 atggcggggg caatcgcttc cagggcaagc aggatacgac tgatatcgaa tccctccata
    13741 cccattgcaa atcccctgcc ttctttgccg atcagataat aatcaggaat ttccacatct
    13801 tccatgaaga tggagcctct ggcgaccgag cgtacgccca tatcctcata ggattgcaat
    13861 gtcacgcccg gatagtccgt cggcaccacg aagcaactga cgcccttggc accggcggcg
    13921 gccggatcag acttggcaaa gacaacggca aagtccgcgt ccatcattgc cgtaatgccg
    13981 gccttctcac cgttcaggat gtattttgcc ccctttttga tcgccgtcgc cttcatcgcc
    14041 acggcgtcgg tgccggaacc tggttccgtg atggcaaagg cgggaatctt atcgcccgcg
    14101 aggaaaggtt gcaggaaatc ctttctcagg gtctcgtttc cctgggcgag aatctccgca
    14161 tccagatggc ccaccacgaa ggttgacatg gttatactgc aatcgccccg cgatacctcc
    14221 tcgcagacca tcccttccgt aacatatggg gcgcccatac ctcccttttc cggaggaacg
    14281 gtcatgccga tgaggcccag ttcgcctatc ttcttgttga tatgctttgg atatttcttc
    14341 tcgcggtccc attgcgtata atagggtgcg acttccttct ccgtaaatct tctcaccata
    14401 tcgcgcagag caacttgttc ctcattaaat tcaaaatcca ttataactct cctttttatt
    14461 ctccattcag attttcatta cgggcattaa accatattaa gaccaccgct cacactcaga
    14521 acctgcccgg tcacatagct cgccttgtca gaagcaaaaa aggccactgc tccggcaatg
    14581 tcttcgggct gggccaagcg tcgcaggggg atagcctttt tgaggccttc ggcaatggcg
    14641 ggattgtcgc cggcaacggc agcgaatagc ggggtgtcgg tcggtccggg agaaacacag
    14701 ttgatcgtaa taccataacg cgccacctct cgcgctaccg ttttcgaaaa ggcaatgatt
    14761 cctccttttg caccggaata gacagcctca ccgcttgatc ccacacgacc ggcatcggat
    14821 gcgatgctga cgatcctgcc atacttgcgt tcgatcatta cgggaagaac cgccttgaag
    14881 cagttaatcg ggcccctcaa attgatggca atcaccttat cccaagtagt aggttcgctt
    14941 ttgatgaaag gctccatctt gtcccaaccc acattgttta caatgacatc aacgcagccg
    15001 aattcggcaa tggtagcttg caccatcttt tcacactggg acagatccgt aatatcagcc
    15061 gtaactgcaa tggcttgcat acccatcgcc ttgatctccg cggctgtttt ttttgccgtt
    15121 tcgggattca aatcggctac gcaaacctta gctccttcgc cagccagctc aagggcaata
    15181 gccctcccga tcccctgtcc cgcacctgtc acaatcgctg ccttatcttt caatttcata
    15241 tgtctatctc ctcaattata atcacttttg tcggcataga attgttttac ttctttccgt
    15301 aagtccccgt atccgtcaat tcaatcaaca ggcccttgcc gctcttgggg tgaatgaagg
    15361 ccacccggtc aggtccgtcg tacatctttc cgatgatctt gagccctttt ccttccagtt
    15421 ccccaaccag tttctccaga tcatcgcaaa gaacggagat gtgatgaaaa ccgccagtgg
    15481 gggagttctt catgaactgt gcgatagcgc catcaggtcc ggtgggctgc atcaactcga
    15541 atgtcgtatc tttgatcttt acggaggaag atacctgctg tagctgagga tagtcgatcc
    15601 gctttacttc ctcggcgccg aatgcatcct tcataaaacc cagcgtctcg tcgatgctct
    15661 tcactgcaat tccgatatgg tctattcctt taatcattgc atactctcct tttttagata
    15721 gtttctctac agagggacat ttccatgctt tctgggaatc ttttcctgtg gcttattcca
    15781 gagcaatttc agcgcacggt aaaggtgcgc cctcgtatcc tggggtctga tgagaaaatc
    15841 gataaccatc ttgccaccgt agagataggg ggaattaaat ttgttgcgat actcctcaat
    15901 tttctcctgc ttgaccgcgc tcctgtcttc cgcggcctgc agttccttcc ggtataaaat
    15961 ttcgacggcg ccttcagcgc ccataatagc catctccgca gtgggccatg cgtaggaaaa
    16021 atcggccccc atcgttttgg atcccatggc cgcccaagag ccgccataag ccttcctaac
    16081 aacaacgaga agcttaggaa cttccgcctc gatatatccg tatagcattt tcgcaccatg
    16141 ccggataatc cctttatact cttccttcac cccgggcagg tagccgggga catcggctat
    16201 tgtcaggatg gggatattaa aacaatcgca gaaacgatag aaccggcccg ccttatccga
    16261 ggcatcactg tcaatggcac cagccatgac catggattga ttcgccacca cgccgatggg
    16321 ataaccgccg atgcgcaaga atcccgtaat catattacgt gcaaaccgcg gctttacttc
    16381 aaagaaatcg ccttgatcgg caatcgagcg gatgacgcgc ttcatatcga agggcgccct
    16441 tggcgcatcg ggaacgattg tggcgagatc ttcgcagatc cggtttggat cgtcgcccgt
    16501 ctcgacgatg ggcggctttt catcgcagct tgagggcaga aaactcagaa gtttcttgac
    16561 atcatcaagg catgcctgct cgcttttcga cacaaaatcg gcgacccccg atatctgact
    16621 atgcaccttc gggccgccaa gctcatagcc cgtaatatct tcatggcaga cttccttgat
    16681 gactcgcggc ccggtgatgt acatttcact ggttttatca accatgaaaa taaaatcagt
    16741 cagggcaggc gaataacagc caccgccggc acatgttccc aagatgcagg atatctgggg
    16801 aacaacgccg gaactccgga catgctcata aaaaagaagg ctgtatgtcc cgctcccttc
    16861 ctggattctt gccccggagg tatctactaa acctatcatc ggcactctgg tctttgcagc
    16921 cagcttctgg gcttgcgcga tcttggaggc attgctcaaa ccaatgcttc cccccaatac
    16981 cgtaaaatcc atggcgtaaa caaaaaccgg tcggccctgg acattcccgt atccggtgat
    17041 gaccccgtcc ccgtaaggac gtttcttctc catcccaaag tcatggcact ggtgttgaga
    17101 atacatattg gtttcaacaa atgttccctt gtcgaagaaa agatcgattc gctcccgggc
    17161 cgtcaaccgc ccttttttat gctgatcgtc cgtcgctttt tgcccgcccc cggcctgcac
    17221 ctgtttctcg atccgctcca gatcttctat caattcctta acacccatga gtttctcact
    17281 tttcaatttt gtggggagct tatgcagctc cccacttgat tattaattat acttcttcca
    17341 ggttgccgac tttccactgt ttcaggtcga cgcccgctcg ctccagctcc ggacccagaa
    17401 ttctgacggt attcttccag aacatggatt cgaggacacc ttcacgccat ccgaactgca
    17461 tgtgctggta gaggatcccg tccaggggga agaggttgaa ctcggagccg aacatgtatt
    17521 tgtccttctg acggcggttc gcctcataga ctgtcttggg ggcgttctcc ggcaggtaat
    17581 tcggccaaat acccgaaagc tcgcggaaga tgttgccctt gtgacggcac agcagcacgg
    17641 catcttcatc ccggccgtcg gagcagtgca gcatgatgat cttcaaattc gggaaatccg
    17701 cggagatatc gtcaatatca gggatgagct gcatcgtgta tttgattttt gtccccaggg
    17761 cacccggcgc cccgcttccg agcccggtaa agccggtatg aagctggatg ggcatgccca
    17821 gttcctggca ggcatcccag agtggataga actgtctgtc gttaacgtgg aaggcctgcc
    17881 ccacctgctg gaattttaac ccgacgagtt taagttcctt gcagcagcgt tccgcctctt
    17941 tgatcgcttt ataaccctgc cagggatcaa cggagcccca tccggagatg acggtatccg
    18001 gccacttgtc gcgcatggcc gcaatctcgt catttgtatt atcggcgtag agaggatcgt
    18061 ctcccatcgt cgtctgcgcc gcccatccgc agggcaggac tttcacgccg agttcctgga
    18121 aggtctttac ccactcttct tccgatggca gagaagccga tatttgttcc ggcgtgatat
    18181 cctttttgcc gtaaaggtat ttctccagat gcacccggaa ttttcccagg cttcgccagc
    18241 cggtttttgt gtagggatgg gtgtgaatat caatgatcgg gggcatccct ttccttgcgc
    18301 ccataatcat cgcattcagt tcaggatcca acttcgtgaa attcttttta acaattagtt
    18361 tatcaaaatg tttcttctgt tcatcgtcca tctctaacat gccataacgt tttttttctg
    18421 ccatacactg ttacctcctc cttgttttat taaatcgctg ttaacgataa caccgctcta
    18481 tgacaattta aatgacccga gatcaaccgc tccacggttt gtcatcttta ttaaaacaat
    18541 cagatcggaa gtggccttcc gcgtccaaaa tcgactccgt cccctgagca gctattaagg
    18601 tggttaaggc cgctcccggg gcctcttgcc ccttaatcga ggacgatcat gacgtcgtcc
    18661 ggttcgacct cgtcattctc tttgactctg atttctttga ccaccccgtc cacgggggct
    18721 accatcggat tttccatttt cattgcatcg agcgtcagaa gaatgtcatc ttccgcaacc
    18781 ttatcaccta cttttacaag aatttttata attttgcctg ccatcggtgc taatatttct
    18841 tcactcacaa cgctacctcc tttaaaaaat aatccgttta ttaaagttgc tcggactcct
    18901 cacgaaaaac ctcagccagt ccggctttcc tatgtgttca tcgccttcaa cttttcgatc
    18961 aatatgggga cgacggcctt gtaatccgcg acgagtccga gatcggatat tttgaacatg
    19021 ttggcgtcag gatcacggtt gatggtcacg atcttcttgg agctgatgca accggtaacg
    19081 tgctgcgccg caccggagat tccgatggcg atgtatagtt ccggactcac gatctttccg
    19141 gtttgaccta tttccatact cagaggcacc cagttttcgt ctacgggaac ccgggtcgct
    19201 ccaaccacac caccgagcag gtccgccaat tccttgatca ttgcgaaacc ttccgtaccg
    19261 cccaaaccgc caccgccgga tacaatcacc ttggcgtctt cgagtttaat gccctccgct
    19321 tccgtcttct tcgcttcgag aagcttcact ttgatgacgc cgggggccgg agcttccgca
    19381 atggttaccg tttctccctt gcgtccatcc tgaagcgccg ccgcctgcat agacttcgcg
    19441 cggacggtgt tgatctgaag cagtcccttt gtcgatgcaa gcaccgaaag ggccttcccg
    19501 ccaaatgccg gccgggtctg ctggaagcgc ccgttttccg catcaaatac tacctcaacg
    19561 caatccatgg ccagggccgc cccaagcctc gccgccacgc ggggagcaac ttcgcagccc
    19621 agatccgtct gtcccatcag acaaagggcc ggcgctatct ttgcacacag gccgctcacc
    19681 agctccgtgt aggcatcgct gctgaattca ttgtatgcgg cattggtagc cacatatacc
    19741 ttatccgccc caagggaaat accttcggag gcaagcgccg cagcgatttc accgagcagt
    19801 agcaggcaaa catccccacc cgactgatcc gccaacactc tggcgccgcc gagaagttcc
    19861 cgggtgatgg aagccatctt gcccttctcc gtttctccaa caacaagtat tgctttacta
    19921 tcagccattg ttcattcctc tcaaggttta atcgcaagaa ttttttccgc cagcagagcg
    19981 gcagcttcgg ccgggtcctt acccgtaaca tattcacagg tcctctgata ggacgggata
    20041 aatagtttaa tgaggttgtt acgggcggca gcggcgcctg tttttgaagc ctccgcacct
    20101 aaatcggcgg cgccccattt ctggaactgc tttttggccg ccataatgat gccccgaccg
    20161 gcgggaatgc gggcctgacc catctcattg ctcatggtca caagagcggg aagcgccacc
    20221 tcgaagacct cggagccatt catgacgacc cgctccacct tgattttgcc gtccaggacc
    20281 tcaatggact tggcccggat cactactggt atccccaggt attcggccat cgcggatccc
    20341 accatcccca catcccaatc tgccgcctgc cggccgcaga ggatcatgtc aaacgatccg
    20401 attttcttga tcgcctgggt taagatgtat gccgtggcaa atccgtctga atcctcaaag
    20461 gcgggatccg tgagaataaa accttcatcg gctcccatgg ctatggcttt acgcattact
    20521 tcttcggtat acttcgtccc caaggtaaga accgtcacct ttcctccctt ggcttccttg
    20581 accctaaggg cagcttccac cgccagagca tcatagggac tcatcaaaaa tggcatcccc
    20641 tccgggggaa tcacctggtt cttcttaccg tctactgcaa acttgagcgg tggaagttcg
    20701 ggatcaagaa ccggcttgat acagacaatg atatccattt ttcctccttt aaccgtattg
    20761 ataacatact ccgaatcctc cgcggggatt gacccaggca atggtgccct tgtcgcagga
    20821 gaatcggcag cttccgcatt ccagacatcc ttcgtaagag aaggtcacat gaccttccgc
    20881 ccgcaggaca taacactccg caggacaggc atacaggcag accttggatt cacatgcttt
    20941 gcatttttcc tcgtccacct ggatgtgtac ctcgcggtcg atatcaaatt tattacgcgc
    21001 taatttttct tctattttca cagtgccttc ctcgctttca tggcatccgc agcaatctgc
    21061 cagagactga ctttgccctt catcgattcc atgacgacct ccaacgtatt cctcctcggc
    21121 ttgccgtcgc tgtggaagat acttcccatc aaaccgcacg caagctccgg ataggcatta
    21181 tagatccgtg gattctccag aaaatggggg gcgcggcgaa aggtttccag atccttgatg
    21241 acgaaattac tcttgagccg ttgctcgtag tcgttcaacg atgacgacgt gaaatcaccc
    21301 ttttctttgg ccttgataac ggtttctccc gcggcaatcc ccgcggccat tgcaaagttg
    21361 gcgccctcga ggataaggcc tgttcccagt accatcgccg ccgagtcacc cgcgacaagg
    21421 ataccgtcgg cgtatagctt cggcatcata ttaataccgg aaacaggaat gagatgggcc
    21481 gaatattcca gcaactcccc gttgcgccac aggggccgta cggcgggatg ttccttgaac
    21541 tcatcgatga gatcggaggc cttcaccttg ttctcgagca gggcgctcaa ttgtaccacg
    21601 acgcccaccg aaagactctc ctgattcgta taaatgaacc cgcccccggg aagcccgtgc
    21661 gtacaggagc ccacaaattc catcacgacc ccttcatttc cgctgacgcc aaaacggtct
    21721 tcgatcacct ctcgcggcag ccggatgact tccttgaccc cctgcttaag atcgacggcc
    21781 ttgaaatcac cacgcagttt ggctttcgtt gccagcagcg agttgacgcc gtccgcaagg
    21841 accaccacgt tggccagaaa gtcctcatcg ccgaccttga cgcccgtgat cctgtttccc
    21901 tcgaagataa aatcggttac ttccagaccg gtcgcgataa tcgctcccgc ttcctccacc
    21961 ttcgacccca tccaccggtc aaacttggcg cgcatgataa cgaaaccggg cgcttcgtca
    22021 aaatcgccgg gggaagactt gaagtcaatc gacagacaat cctctttcgt catgaaagaa
    22081 atgacatggc gtttgaggaa tcgttcaacg ggtgcttcct tccagaaacc cggaaatagc
    22141 tcgttcatga cgggaccgaa gaaagccccg ccccacatat ttttagctcc cacatattgg
    22201 ccgcgctcca gaaccaggat attcaggccc gccttggcca tcgtataggc ggcagcgcag
    22261 ccggcaggac cggcacccac aattatcgca tcaaatatat ccattttcaa acctgctttc
    22321 tgaagaagcc gttgcagaaa tttatttcgc taccgcactg atccccgttt cgagacgccc
    22381 gaggaccagc ttgtggacat gggccgtgcc tccgtaaatg tacggggcca tgcagtcacg
    22441 atagatcctc tctacggggt attcgtcgct gagtccgtag gcgccgtgca gctccacggc
    22501 gtcagaggcg catttcttga gcatcagcat gttgtggtat ttggcaaccg atgacatctt
    22561 cgtcacatcc actttcttgt ccttctgaaa cccaagcttg taagccagca actgggacgc
    22621 ttcatgggcg atgaccatgt cggcgatctt gtcctggacc atctggaagc tggcgatggg
    22681 gcgtttgaac tgaatgcggt ccttgcagta gttgaccgtg agatccaagc aggccttgat
    22741 cgctccggcg gcggcgcagg cgatgccgta gcgggtgtgc tccacattgg agaaggccgc
    22801 ccgcattccg taatgaattc cgaacccgcc cagttgactg tctttcggta ctcggcaatc
    22861 ccggaagaag agctgcccgt ggtcgtggtg acggaaggcc atcttgttct cgatcggggc
    22921 gcgggtgaaa cccggagtac cccaatccac acacatgact accagacccc gggttccttt
    22981 cgtcttgtcc gtctggcaga cgaccatggc gaaatcggaa tgacaggcat tggtgatcca
    23041 ggtcttgttt ccattgatga cgtaatcgtc gccgtccacg accgcggttg tctcaatggc
    23101 cgtggcgtcg gatcccgcgt tcggctccac ggtcccgaag cagtgggtct ttatcccctg
    23161 gatcaggccc ggcaggtatt tctgcctctg ttcgtccgaa ccactctgga tgagaggcag
    23221 cattcccaga gagactctcg agaacatact catagcaagc tgcagatcat aataggccat
    23281 ctcctccgtg actataccga cggtcatcca accgagagcg agaccgccat attcttcggg
    23341 cgcgttgatt cccagaaaac cgagctcccc catcttctgg tatatctcgg ggggcgtccg
    23401 gcagtctcgg tcccactgtt tcacgtaagg ctgaacctcg cggatggcaa actccttagc
    23461 catgtcttta aacatcgtct gctcagtagt taactcaaaa tccatctctt tctcctttca
    23521 atagttcaca ttaatggact caatatcgtg cagttaggaa gtgattatac cgaaccggac
    23581 cgaaatcctc gttgagaaat cccgctgcta aaactgaaaa aaggtcaaat ttagactata
    23641 ttgcagttag atatgtactc aagagggata gtgcaaaaga caagccaact tgcaattaca
    23701 gcacttgtca tcaaaatcat tgatgtattt gctttaaacg gcttgtgcta aaaaagatgg
    23761 gacgccgcta ggttttgtag ttataaagat acacaaaaga cggagaatgc ttttgtgacg
    23821 gcctttacag acgcgtcttg ttttcgcctc atgtagtgat aaccatacat tacaacggga
    23881 tgttatatct tttgattttt tgatatagaa ccgttctgtg cactccgaga atttttgctg
    23941 ctctctgttt gtttccttca acggaccgga tcgcatcaac aataactttt ctttccgttt
    24001 cagcgacgat atcccgtaaa ggaccgatgt attttctatt gccgttgctt gatttcttgc
    24061 ccaactcttt gatccgcgtg ggaaggtgct gcaactggat ttcaggccct tcggtaataa
    24121 tcaaggcccg ctcgataaca tttctcaatt cccttacgtt tcccggccaa ggatatcgct
    24181 gcagagcaat catggctgca tccgtaataa gtttggccgg catattatct ttctgactca
    24241 attcattcat cagaaacgag gcgatttcag ggatgtcttc ggggattgac cgcaagctcg
    24301 gagtattgat tgtaaatatg ttcagtcgat aaaacaaatc cgtccggaaa gtaccctttt
    24361 taatcatttc ctgcaaatct ttgttcgtcg aggcaataag acgaaaatca actttcatgg
    24421 gctttgtgcc accgactcgc tcaatttcct tgtcctgcaa tacgcgtaaa agttttgcct
    24481 gcatattcag cggcatatcg ccgatttcgt cgagcaatat ggtgcctttg tgtgcgagtt
    24541 caaatttccc gggtttacca ccctgtttag ctccggtgaa tgccccaccc tcatacccaa
    24601 aaagctcgct ttcaatcaac tcggcgggaa tggccgcgca attgaccttg ataaaagaac
    24661 cgtcggttcg cttgcttgct ttatgaataa aaatggcaca ggcttctttg ccggtcccgg
    24721 attctcccgt caccaggacg gtcgcatcgc tctttgccgc ctgtagggcg ttgttcatcg
    24781 tctcctggat aagttgcgac ttacccgcca caagtttatc ataggcataa atattctggt
    24841 tcgtagcttc tttacgataa tactttaact gcccttccag tatattcact tttttataga
    24901 ggtctttgat cgtatcgact ttgttgaata tccttttacc gaggacaccg atgattttat
    24961 tgttctcgtc ccgcaacgga atccttgcca cgatctgctg acggcctcca agctggtacg
    25021 ttgcaccgat ttctaccttc cctgttatca aggtttcatg catgccactg tttttattga
    25081 cctcgacgat gtgttttcca atcgcctgct ccggagtcat accataaagt cccacattgt
    25141 gtcggctcat gaagcgaacg atgccgtctt tatcaatcac tatcgggcat tcgtaatgat
    25201 tgtcgacaaa ggcgtcaagc aaagtatcgc taatatacga caaccatttg ggtttcgaat
    25261 cttctttcat gacaaacctt ccaacacctc tgaaggagag agcagctttc cttaggacta
    25321 tcgggaggtt agtttctaca accattatca catttgtcaa ggaataaata atggtccgca
    25381 tttttcgcgt tgcaatccgt atcgtttata tgtaacttgc tgccaccaag aaaataaagg
    25441 atgtaaatca aaatggaaaa tggacatgac ttacaaaggc tattttatcc tcggcgggta
    25501 actatcgtag gggccggtga caaccccctc ttaatcaatg gacgcagtct gatgttcatg
    25561 ctacgccaca aaagcaccgc tcgcatatac cctgttaatc ccaaccgcga caccgtacag
    25621 ggaatcccct gttaccggcg gatagccgat cttccggaag ttcccgacgt tgccgtcatc
    25681 gtcgtgggga aaaagttagt acccgccgcc attgaagaat tggggcaact gggctgttcg
    25741 tttgccatca tcaatacatc cggttatgcc gagacgggac cggaaggact tgcggaacag
    25801 gaggaactgc tgcgcagatc ccgccggtac ggcatgcgac tgatcgggcc gaattgcctc
    25861 ggtctcatca atcttatctc tcccgtaata ctgagctggt gcgcaaccct cgagagagaa
    25921 cagggagatc ttttgccggg agatgtggca ctgatctcac agagcggcgc catgttggga
    25981 tccatatggg accgcgcgat gggattggga ttgggctact cctatctttt atcatccggc
    26041 aatgaggctg atctcggaat ggctgatttt atggagtatt ttgctcagga taaaaacaca
    26101 aaagtgataa cgggattcgt tgaggcactg cgtaatccgg aaaaattcct gcaaggcgta
    26161 gatcaagccc attccaacgg taaagccgtt gtcctttata aggtgggaag aaccgcggaa
    26221 ggaaggcggg cggccgtttc tcacaccggg tcattgacgg gatcggatga aacttttgat
    26281 gccctctgtc gcgcccacgg aatcgttcgt gttgatcgtt tggatgcttt gattacatcg
    26341 gcgcttgcgt tgcgcgccct gcctccggcc cggggaggcc gcctgggaat tttctgctgc
    26401 tccgggggcg ccgccggttt ggctgcggat cagatgcgcg gaaccggact gtccattgca
    26461 ccggtgggcc cggcctttga gcaggacata accaacatca cgaattttca tcccccccat
    26521 aatcccttgg acatcatcaa agggccgcta aaatctttcg atgtcattac ggaggccatg
    26581 aaaaggttcg tccgtgagga taattttgat cagatcatta tcttgatgac caccatgtat
    26641 ctgcaaaaga tcgcaccgaa gttgatgctc acgggtttga aagacgggcc cgaaaaaccc
    26701 gtactcgcat gttggctggg gaataaagtg gtagcgaagc cgagcgagga aatgagaaat
    26761 ggcggcgtca tcacctatca cgacgttgag tcttgcctcg aagctgcacg ggcattggct
    26821 gttatcggta aacatcgccg gcgcctggcg atggaattat catataagca gcccccgcga
    26881 gatgcgaaaa gtggcgcgtt aaaaatcatt gaacaatgcg aggggcgatt ggatgaggca
    26941 tcttcaaaag ccatcctggc gctttacggc ttttccactc caagaggaaa gctcgtacga
    27001 aatcttgacg atgccatagc tacagcgctg gcactgggat ttccggtagt tgcgaaaggc
    27061 atctccccgg atgtccttca caagacggaa gcaaaagccg tctgtttagg catcagcagt
    27121 gaaaaagcgc ttaagaaggc ctttgagacg gtggcaacgg caatcgagtc gagaggaact
    27181 ctgcgcggcg tcttgatcga agagatgctt tcggaaccgg ttgccgaagt tatcgtcgga
    27241 tgttccttgg acgagccatg ttttcataaa attattttcg gtctgggggg aatctgggtg
    27301 gaagccatga aagacatcag tgtgagaatg gctccgattc tggaagaaga tgcggaagag
    27361 atggtcgcag agattcgcgg cgcaaatatc cttgacggct tgcgcggcaa gccatccgcc
    27421 gataaaggag ccctcgtcag cgccttggtc tctctctcgg cactgatcac ggacctgcga
    27481 gaagaaatcg gggagatcga tgttaatccc cttatggttt atcaagaagg cgcggttgtg
    27541 gcagatgccc tcctgtcctg ccgccggagc tgaaaccacc gcatatagag ttcaacgtgc
    27601 tgcttgcgtc tcattcagtt ccttcaggaa ttcttccagc gtcatgattt tgaagagggg
    27661 ataaagtgga ttgctccgat acatgttcat cgccgcccgg tgaatttcct cactgtaact
    27721 ggcgctgcaa tcctcgagaa tataggttcg aaagtcgttg gacagggcat caaaagcgga
    27781 gctcaacacg caccagtgcg agttgatgcc ggtaagaaca acggtatcca caccgaacag
    27841 gcgcagggtc tgatcgacgt ccgttttgaa aaatgtgctg aatcggcgct tgtagctgta
    27901 aatatcttcc tcagtatggg acagaccatg gtatagctcg gcgcctttgg ttccccgaat
    27961 ggagtgttct ctcatctttc ctttgaagat aaaatcccca cgcataaaac tatccataga
    28021 aaaaataacg ggaatgttct tgctccgggc gatatccgtc aggcgattga tattggggac
    28081 tatcgctttc gcaaaaggcg tcacgggatt acgatgccct tccgaaaaac tgtcttcgag
    28141 catgtcaata acaataatgc atggattcat tttattcctc ccgattttct tattttatca
    28201 atgaacgtgt ccccctcttc tccacgcaag gcccgtatca catgaacggt atgggcatgg
    28261 gcgacctttc tccccgcctc atcgaatata tcggcttcga gaacggccga gtggcgccct
    28321 ttatgtacga cccatccttt tgctttcaac atcccacctt ccacctgcgc aagataatac
    28381 acctcgaggc tctgggtggc gaaggtttcg ttctctttga tgaccgtcac gagcgacaga
    28441 ctgcaggccg aatcagccag ggaagcaatc gcccccccgt gcgctcgccc gtaaacgctt
    28501 ttgagactct cccgtatctc catggccacc tcactgcccc ttgcggataa ttcgaccagt
    28561 ttgattccca gatgccgata gaagggagat tcattaatga tatctcttat cttttcaaac
    28621 gccaactcaa gtgttttcaa tgtcaccccc gatcactcat accgataccg atcccatgat
    28681 ctttgattat tcctcaatgg ccgccagatc cacgaagcct cgggaatgaa gaatataggg
    28741 ctgagcagac cgggggattt tcagaagcga cttatcatcc gtatagcgac aaaacggtgc
    28801 ttaaggcatc gcccgccata cccatcgatc aacggcatcc ggtaacgaac tgaccactcc
    28861 acccggaagt gcccccttat cccgcagagt aagatcacca tcactcgctc gcaatggcgc
    28921 tttcaaccag cgctccacgt taaacagcac ccagtcgatc gtgcagttat gtctcagagc
    28981 ccgctatttg ctcgacagtt tggccatcat tttatttact gcctcgtgat gatctttcaa
    29041 cgtgcgaata acccccacat gagatgatat caggtctaag gcagtgcgaa gttcaacgcg
    29101 gctgctctga taggttgccc tcttggtggt ccggatagcg agttcgggac tgtcggctat
    29161 cttgcgggca aaatcatagg tctcttccat taatttatcc gccggataca ccctattgac
    29221 cataccgatg cgtaaagctt cccgggcgtc aatgaaatcg cccgtccaga acaattcgag
    29281 agcttttgca accccaacga gtctgggcag gaaatagcac cccccatcac cgggaacaac
    29341 tccaatgcgt atatacccct cggaaaaacg agcggtatcg gcaacaaatc gcaaatcgca
    29401 catcagggcc atgtccatgc cggctcctac ggcgatccca tttaccgcgg caatgatggg
    29461 tttatcgata tcctccataa tgagggccac tctgtggatc tgctcccaaa gcatgttctt
    29521 gtgatcgatt gcacaattct cggcatcccg cagtccggct aggtcagcgc cgctgcaaaa
    29581 agaatcaccg gttcccgtca cgacgataac cttgacgtca gcatcttttc ttgatttctc
    29641 taaggcttcg acccattgat caatcatgtc catcgtaaaa gagttctttt tcccggggcg
    29701 attcaaacga atcgtggcaa tcccatcact cttcagaaat aataaatcct tattcccttt
    29761 ttttgatgca ttatttttta cattccttgt ttccacttta tcagacatcg tatatttctc
    29821 cttgaactct attcactccc taaaaattcc gtatcgctgc cgcacacaaa agccgttcca
    29881 tgaaaagatg cttaatctct gctcatcctt ccggtaaaac atggcggcat gatatgttta
    29941 ctccctacaa cagaaaatga tcgttgtgca acaaataatt gctcggcgtt aagacattat
    30001 agatgaggat tatcccttga cttgagaaaa ttacgcctct atatatcacc ccgtcggaag
    30061 cggcttctta ttaatttgtc aaggtcggcg gtaaggttcg gcaaagcagg atcaaggccg
    30121 atcggttatt gatggttccg gcgtgcccgg atgaaagggg tttgtgttga tatgattgac
    30181 tgggacttgt cgataaaaac ggcggtgggt ggattcggct tggtgtttgg catcctgatt
    30241 ctgctcatga taacggtaga gttgacacga gtaatcaccg agaaattttt cggtggcaaa
    30301 aagtagttac tgttaaattt ttaaggagga acggacgtgc tttttggatt aatggagaca
    30361 gggttcccgc tgttttactg gggaaacgcg ctgatgattg taatagcact gatactaata
    30421 tatctggcaa tcgccaagga tatggaaccc ctgttgctgg ttcccatcgg attcgggatg
    30481 attctggtca acctgcccct cgggggaatg atggactacg aaatgaggct gaaggctcct
    30541 caggctggca cggtaatgga gatatcaaca caggagggga aaggattcga taagggtagc
    30601 gtcatatgca agcttgattc gggcgaggtc gtcgcccccg tcttcggacg ggtcggcgaa
    30661 ttgattgttg aaaaaggaca gcatgttgaa aaagggcaag tggtggcctc catctttacg
    30721 atggagcaga ccgaccaggc agtgtatcct tcacgtcctg tagggcttct ttcccgaatc
    30781 tttcagtacg gtgtgatgtg gcagattctg cctcccttga tgttcctgtg cctgggcgct
    30841 ctaaccgatt tcggccccat gcttgccaat ccaaaaacac tgctcctggg ggccgcggcg
    30901 caattcggtg tctacctggc attcttcatg gcgcttttcg ttggattcac ccttccggaa
    30961 gcggcttcca tcgggatcat tgggggagct gaaggaccta caaccatcta tgtcagctcc
    31021 gtgctcgctc cacatatcat cggggctacc gctgtcgctt gttattctta catggcactg
    31081 gttcccatca tcattcctcc cgtaatacgg ctcctgacca cgaagaagga acggggaata
    31141 ttcatgaaac cgtcactcag aaaggtttcg aaggtggaaa agatagtctt tcccttgatc
    31201 acagcgatta tagtcattct gatcgtaccc acctccgccg ccctgatggg atgtttcatg
    31261 gtaggaaacc tcttcaagga atgtggcgtt acggaacgac tgagaaaaac cgcgcaaacg
    31321 acctttgtcg atctcatcac catttttctg acccttgcca tcggtgcgtc aatgccggcg
    31381 cagaattttc tgcaacccaa gacgcttctg attctcgtcc tgggcgttat ctcatttgcc
    31441 tccgcagccg gggcaggcgt gcttctcgcc aagtttatga acctcttcct gaaggtaaaa
    31501 atcaatccga tgatcggagg ggccggtgtc tctgccgtcc ccatgtccgc tcgggtcgtt
    31561 caggtaatgg gtcagaaaga gaacccaaag aacattttgc tgatgcacgc catgggacca
    31621 aatgtcgcgg gtgctatcgg tgcggcaatc gccggtggtg tcttcatagg aatacttgga
    31681 tagaagactc acggtacaaa gatgtaaaag tattaatatt tgtcttttaa taaaccggaa
    31741 tagaaaatca gaggggagag acgttcgtgt cctcccccgt ttttctttaa acaaagagtc
    31801 ggcggcgtga gcagacccgc gtttctctac cggattttct tatatcgcta aaatcatgtt
    31861 caagacaaaa cgtcattggt ggtggatcat cgggacttgt atcgctttat cgggagtagc
    31921 gattgtaagg ataattgccc ctcaatatcc cctcggcatt actcgaatcg caattgcatt
    31981 catcggctac aatattatca ttttcggtct atattctcta acttatgcaa cgcgaagaga
    32041 cgaatgactt gaagaatatc ttattgatat tgttatttat taaccgaaaa aacctcgaaa
    32101 cccgcgcgta cccgcaatat ccgttttcgc tcaatgattt catatagtta agagctatgt
    32161 aatttacata atttgaaaac ggcctcggga tgaaagcgcc gtcattcccc cgacctcgga
    32221 ataatttcag agcttacgga cagccacccc cgcccccgcc tgggccagct cctcggccag
    32281 cgcggccaga tggcggtggt gggtgaagag gatcacctgg gtgtgccggc ccatttcccg
    32341 gaggacccgg agggtggcgg ccgtccgttc ctcgtcgaag ttcaccagga cgtcatccac
    32401 gatgaagggc agcggttccc cctggaaata ggtctccgcc gtggccaggc gcagggccag
    32461 gtagagctgg tcccgggttc cggagctcat gccttccacg cccaggcggg cgccgtcgct
    32521 ccgtaccccc atgagcaccg gctcgtcctc gggaccgatg tcggtcatca ggcgcgggaa
    32581 ggccccggcg gttatccggc cgaaaagctc cccggcccgg aggagcaacg gcgcctgatt
    32641 cttctcccgg tagcggttca tggcgttttt caggaggtgg aaagccgccc ggatccgggt
    32701 gtaccgggcc acgtcgccgc gcagggaggc cagcgcggcc tgggcctgct cctccgcttc
    32761 cgcggcggcg gaagatccgt ccatgcgccg cagctccgcg ttttcttctc cgatttcggt
    32821 attcagctcc tggcgccggg actggagttc ctcaagccgc ccgttcactc cggtcagggc
    32881 cgccggcagt tcatcgggag acacggcggc ggcctccgct tccagcacgg taaccggcag
    32941 gccgtcgccg ttttccagga gaaaacgttc caccgcggcg aggcgctccc ggcagccctg
    33001 gcaggccacc gagcgggcct ccgcggcctc gaattccgac gtctcccggc accgggcctc
    33061 cttcaacagg tccgcgaccc gttgctcctt cacccgaaag tccgtcgccg ccgcccgcag
    33121 ctcctcctcc gcctcccgaa cccgtttggc cccgttctgt ctctctttct cccggagtcg
    33181 cgccccgtcc acctcctgct tcaaacgccg gaccacctcc tcgggcgggc aggcggcgta
    33241 ttccggaacc cggcgccgga cgaattccgc cactttctcg gtgaaccgcc tggcctcttc
    33301 gttgatagcc cagatacgcc ggtccatgtc ggcaatctgt ttggtctggc cgaacagggc
    33361 ggtgtactga tccagaacgg acagcgccgg ggcgggcggg gtgtccagcg acagacccag
    33421 tccggtcatg gcggcccgcc atcgctcctg ccagtcccgc agttccgcgc gggcggtttc
    33481 cttttccccc gccgcctccc ggacctgtcc ggcctgcctg tccatctgcc gcctcatctc
    33541 cccccggcgg cggttccgct cggtccggac ctccagatgg tgttccgcct cctcccggag
    33601 gtcccggaag agttccgatt tccggtgcgg ttcgggccgc aagggccgca aggcctcccg
    33661 gagactggcg caggtggatg cgtgccgggc ctccagggca aggagttctt cccgggcggc
    33721 ctgcgcccgg aggatctctt cccggagggc gtgaaaagac gacagccagg cggccatctc
    33781 ccggggcgag agcggctcga tgccgccggg ggcccagagg gcgtgccagt cgtcgtcccg
    33841 ttttttctcc tgcgctacaa gttccgccgt ttcccgttcc agttgccgcc gccgctccgc
    33901 cagggaatcg tccgccgccc ggagatccgc caggacatgg acgcgctgcg cctcccggcg
    33961 taggcggtcg gcgatgtcgt cggcggcggt gacggcgtct tcgtaggcct ccggcggcgg
    34021 cagatcggca tggaagcggc gctccccatc ttcgttacgg gcgtcgccgg ccagaagtcc
    34081 gcggatcagc cgccaaccct cctcccggcg gcggcgggcc acctccagat cctgctccgt
    34141 gggcaccatg ccggccaggg caaaacccgt gagtttcttc cgcaccgcca gtgtttccgc
    34201 ggcgttttcc cccgccgccc gctctatttc ccggcgccgc tcttcgaggc gccgccactc
    34261 cccttcgcaa cgctcgatgc tttccctggc cggcaccgac aggatgaggg ccgcttccat
    34321 atccccccgc caccgcccga gaccggcgag ggtgcgctgc gccttcccct ccgcctcctg
    34381 gagagccttt cgcgccttga acagctcgcc gtccggatct ccctccttcc gggcgacgcg
    34441 tacggcctcc cgcagcgcgg cgggatctcc cccgtcggga aggccccgca gctcccgttc
    34501 cgtctcctcc tgatcccgcc gtcttctttc cagcgttttt tccgcctttt tcagggaggt
    34561 caggaccgcc tcatgacggc gggccagttc cgtgaccagg gcgcggttcc tttcgacctg
    34621 ggggatgatc tcccccaccg attctagccg gggttcgatg ccgatggttt ccatgaggcg
    34681 ccgggcgtcg ttccgcatct gccgcaggcg gccctccaga tccggccggt cccggagggc
    34741 cttgagatgg ctccccaggt cctgttgcag ttcatccacc aggaccgcct cggcaaggag
    34801 cgacgacggc ggcggcaact gggcgcagac cgcccgcaga ttcgccagcc ggtctccggc
    34861 cctggccacc gcctcccggg catgctccca atcccgcaag accgcggccc gccgggacgg
    34921 gaaatccgcc gccaggatgt ggacctcgcc catggccgcc agccgggcga tgagttcccg
    34981 cctttcggcc agtcccttca gcacccgccc tatccgcagc aggcgctggc gctccccctg
    35041 ccgcgccccg atctctccat cgatccgccg tacctccgcg accagttccc gacgccgcgt
    35101 ccagtgccgg tcccattccc gggccggcag ggattcccgg cgcgcccggt cccgcaaggc
    35161 cttgaattcc gccagtccgg cgttgatgcg cggtttcgag gcccggggga ggaagagttc
    35221 cgcggccgct tcctccaact ccttgaggag ggcgcggaac ttctcggacc cgatggccgc
    35281 cgccagcagc gcttcgccca cccggccccg ctgggtcagg agctcgtttc cgccccggac
    35341 gagttcctcg tgatcgatgc cgaacatggt ggcaaacatc tcccggctca ccccggacag
    35401 aaacggcgcc agttcccggt cgtccaccgc cccgccttca tcgggcgtca gcagggtgtt
    35461 ctttctgccc ttgcggcgga cgaaggaacc ctcccggccg tccgccaggc gcagtgtccc
    35521 gccgatccgc aactggtcgt tgccgtggag aaaaccgtcg cgggtccgtt cggggatgcc
    35581 gtagaggaag tccgtcaggg cgcggagggc ggagcttttc cccgcctcgt tggccccgtg
    35641 gacgacgtgg aggccgggaa gatccccggc aaagtccaag cgggcgtccg tgaaggggcc
    35701 gtaggccttc aggtggagag tcatgatcct catgccggcc ctcctccccg gaggaggcgc
    35761 gattccagta gatcccgggc ctcggcaagg atgtccgcca gccgccgcgc atcgccaggg
    35821 tcaccgcccc ccggccccgg aagcaaatcc ggcggcagct tctggtgcaa ctgactaatc
    35881 tcctcaatta aaccggtcgc ttgcattgga tctgccgcca gaaggcgcag ctccttcagg
    35941 atcgccgcca ccgcctcgtc gccttcccac cccgccgccg ccgcgggcgg ccgggtgtcg
    36001 aaccggatct tttccagcca gagccgcccg tggccttgct cttgggcggc ggcccggaac
    36061 tccgcctccc agtgctcggg gtgggcgcgg aaagaggcgt gggcgggggt ttcaccggcg
    36121 atgagcagcc tggcgatcac cggcaggccg ccgtgccggt cccactcttg cgccagggcc
    36181 cgctgggccc gttcgatcac ttcccggggg cccgccgtcc cggtggcgtc aacctccacc
    36241 ggggaccagc gcaggatgtc caccggctcg tgacgcacct cccgcacctc cccgtcctcg
    36301 acccggacga cgacacaccc cttggccccc gtctcccgga tgtggcgtcc ctgaagactg
    36361 ccgggataaa cgatccacgg atcgcgggcc acgacctcgt ggcggtgcgc atgccccagg
    36421 gcccagtact ggtagccccg gccgcgcagt ccgtcggggg tgcagggggc atagacgtca
    36481 tggccctccc ggccggtcag gctggtatgg agaaccccaa tgttgaacat tccgggccgg
    36541 gccgggggaa atttccgggt tacgtcgtcg gagacgattc gttcggcgaa tccccggccg
    36601 tggaggacca cattcaactc cggcaggggg atgctctccg cttttcgatg ggaaaagacg
    36661 taaacgttat cgggcagttg cagtgatttg gttatctggc tggccgcgtc gtggttcccg
    36721 gcggcgatga agacccggat gcccgccccc ttcaggcggg ccatctggcg ggcaagaaag
    36781 atccccgtgt tgtaatcctt ccagtccccg tcatagaggt cgccggcgag gatgacaaag
    36841 gaggcgtttt ccttgatggc cagagcgacg agattttcgc aggccctccg ggtggccccg
    36901 cggatctctt cgaccggcgt gtcgccgtat tgctccaggc ccaccagggg gctgtccaga
    36961 tgcacgtcgg cggcgtgaat gaaccggatc atgccccctc cggaggaacg ccccgacggt
    37021 tgacggcggg gaccggcaga cgacggacgg ggattggtgc caggtaagcc atgaaaatcc
    37081 tttttcgacc cctgcaaaga ccgataaggt tcacccccct ttgtgacggc ataccgtcat
    37141 gagcgtttcg gataggaaac ctcccggttc aaggagccat taactggaat ggagacccgt
    37201 tccggctaac acttttaccc taaaacatca agaggaacgt tgtcctttta gccctgattg
    37261 tgctatggag cggatcatgg cgccacggat catccccagg aaagagcaca acatctcccg
    37321 gaagcagatc aacccctatg ccctgcgcac cctctaccgg ctccgtgacg aaggcttcat
    37381 cgcctatctc gtcggggggt gcgtccgcga cctgctcttg aaacggaccc ccaaggattt
    37441 cgacatcgcc accaatgcca cccccggcca gatcaagcgg ctcttccgga actgccgcct
    37501 catcggccgc cgcttccggc tggcccacct ccacttcaag gaggagatcc tggaagtctc
    37561 caccttccgc gccgacggcc ccgccgaaga tagcgaagag gaagggaggc cggggaccgg
    37621 ggaggggaaa ggcgccgtcg ccggccaccc ccggcacctc aaggacgaca acggcgtggt
    37681 cctccgggac aacgtcttcg gcacgccgga ggaggacgcc ctccgccggg atttcaccat
    37741 caacgccctg gcctacaaca tcgccgattt caccgtcatc gattattcca ccggtctgcg
    37801 tgatctccgg gatcggatca tccggcccat cggcgatccg gcggtccggt tcacggaaga
    37861 cccggtgcgc atgctccggg cggttcgttt cgccgcctcc cacgacttcg ctctcgaacc
    37921 agccgcctgg gaggtgattc gtcgcctcgc ccccaccatt tcccggtcgt cccccgcccg
    37981 gctttatgaa gaggtccaaa agctcttcct gctgggctcg gcgggccggg tcctcggtct
    38041 gctggaggaa agcagcctcc tggcggcact attaccggga gttgaccgcc ggcttgccga
    38101 cgacgaggtt caccggcgcc ggctgcgggc caatctcgat tacctggacc agcggcggca
    38161 aagcgagatg ccccccacgc cgtcattcct cctggcagcc ctcttcggtc cctccctgga
    38221 agaagcggcc ctggcgggcc accgggaagg aatccccttc cagcaggccc tggacagcgc
    38281 ctgcggcgcc gccctgatgg aactgggcaa caccgtaacc gtcccggccc ggatcggcga
    38341 tcatctgcga cgcatcctag ccatgcagca ggtgctccgc cgccggccgc cgcggcgccc
    38401 cacggccttg tccggccggc aggaattcgc ggaggccctg gagtacctgc gtatgacgac
    38461 ggcagtccag agggaaggac aggctgcccg ggaatggtgg gatgccttcc tggccggcgt
    38521 tccaccggaa aaaatcaccg ggacggcgga tgccgacacc ccggcgccgg aagcagacgc
    38581 ccggaagaaa aggagccgga gacgccgccc ccggcgccgg aaggccgccg tacccgcagc
    38641 ggcctcggcg gatgcgaccg gctgattccc ggtagcactc ctcgccacca cgccgggttc
    38701 ctgtttccga tttttca
//
LOCUS       DAOSMF010000009        37273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_1492_length_37273_cov_25.281692, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000009 DAOSMF010000000
VERSION     DAOSMF010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 37273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37273
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1492_length_37273_cov_25.281692"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(321..614)
                     /locus_tag="PLT63_02370"
     CDS             complement(321..614)
                     /locus_tag="PLT63_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97594.1"
                     /translation="MEFTDVCVEAMDFLLNYAADTVKTNSPEQVRLLLDLSSSSGPML
                     EQLRKAYMDSDKLGSAKEKGCLLDLTVMMEKCIWLLNRLLVLMQTENEPATDN"
     gene            complement(818..1348)
                     /locus_tag="PLT63_02375"
     CDS             complement(818..1348)
                     /locus_tag="PLT63_02375"
                     /inference="COORDINATES: protein motif:HMM:NF022607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2939 domain-containing protein"
                     /protein_id="HOD97595.1"
                     /translation="MKKKAIVITSCVLLSLGAGALYSLPYVAIYRIMAAFENQDIEKL
                     AALVDFSEVRENLKRYLGAKILGDPADDPPAAARDLRDSLVARMVDRAVDDLITPEGL
                     AAYMKERLASLAAAGGLDRPNARALFMAFLRHADFAYTSPSEFTVTMPAGEEGEAVRF
                     ILRRRGLSWQLAHMEF"
     gene            complement(1354..1761)
                     /locus_tag="PLT63_02380"
     CDS             complement(1354..1761)
                     /locus_tag="PLT63_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope biogenesis protein OmpA"
                     /protein_id="HOD97596.1"
                     /translation="MKTMSYLTIGLILLLGGCAWNPVLYPNAHLNKVGQEQAERDIAD
                     CRQQADVYVKSDAAKTVAKDTAIGGAGGAVVGGAIGAARGSFGSDLGVGAAAGAAAGL
                     VTGVIKASEPSAVYKGFVNRCLGEKGYEVIGWE"
     gene            complement(1790..3145)
                     /gene="rsmB"
                     /locus_tag="PLT63_02385"
     CDS             complement(1790..3145)
                     /gene="rsmB"
                     /locus_tag="PLT63_02385"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HOD97597.1"
                     /translation="MVPQQPRRLAVMILNRIDTSGAFAEPLLDRCLSRWRDGDPRDRA
                     LVTELVYGTLRMRGFLDWVLAQFLRKDLAPEETSLRSILRTALYQLFYTERIPPHAAV
                     NEAVALARTLCPGREALVNAVLRGVLRRRDRLPYPERRVDLPGYISVLHSHPPWLVER
                     WLRQWGPEETLALCRANNTIPDTVLRVNRLQTTGKKVIEALAGEGVVAEKTRYAPEGL
                     VVRSAGKLLRDTLSYRRGDFQIQDEASQLIARLVHPAAGEAILDLCAGIGGKASHLAE
                     LTDNRGRIIAVELLTSKIKKLHGLAARLGISVISTVAADATHPLVEVPPGSCDRVLLD
                     VPCSGLGTLRRCPEIKWRLAPGRLARHAALQKRLLARAGDYVKPGGRLVYSTCSIMAE
                     ENEDVVRAFLEQRRDFHRISAEAPASELLDGDGYLRTFPHRHGTDGFFGAVLEKETGM
                     G"
     gene            complement(3187..4125)
                     /gene="fmt"
                     /locus_tag="PLT63_02390"
     CDS             complement(3187..4125)
                     /gene="fmt"
                     /locus_tag="PLT63_02390"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOD97598.1"
                     /translation="MSKPRLLFMGTPEFAVPSLRRLIEAVYPIVGVITQPDKPRGRGR
                     RLQPPPAKELAAAHELLILQPERVRDESFLQLFRGLNPDLVVVAAFGQILPPEILERP
                     PLGCINVHPSLLPKYRGAAPLNWTLIRGETTTGVTIMMMDAGVDTGDIIMRRETPVAP
                     GETYDLLHDRLARLGAELLLETVALLAAGRAVRTPQRQEEATYAPRIRKEDGCIDWRR
                     DAGAIVNLIRGLSSTPGAYTFLGGKMLKIYRAAGRPGDTGAAPGTVGTATKDGLPVAA
                     DDGWVYLQDVQLEGKNRMAIHDFLRGFRLPERSRCG"
     gene            complement(4220..6430)
                     /gene="priA"
                     /locus_tag="PLT63_02395"
     CDS             complement(4220..6430)
                     /gene="priA"
                     /locus_tag="PLT63_02395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOD97599.1"
                     /translation="MFVKVAFPISAEQVFDYAVPASQVAAVAVGKRVRAPFGGKPQTG
                     CIIAISATTDVENLKEITEIIDTRPLFGARELAFYRWIADYYLYPLGKALHEILPGGK
                     NIRSKKEKTVLRIQTAAVPALTPRQRLVLEAIPEEGASDEVSLATLRRNLGDVRPVLA
                     ALVKKGLVEISERETFRPPPRRRQVGPKADRPVPNAGQKAALREIVTSLRRGHFQTFL
                     LHGVTGSGKTEVYLRAMEETRRYGGGVIYLVPEISLTPQLLTRLEGRFGGDNIAVLHS
                     GVSRAVRYDQWRLLESGTLSIAVGARSALFAPVRNLKLIVVDEEHDASYKQDERLPYH
                     ARDMAIVRGRLEGATVLLGSATPALQTYFNTTRGKVSLLVMPQRIADRPLPAVELVDM
                     TMEKAIREEGAGAILSRRLLAAIDVTLQRRQQALLLLNRRGYHTFLVCRDCGRPLRCR
                     NCEVSLIHHAGDNRWKCHYCDYSVTGQSVCSHCKSPRIGTYGVGTERLEDEIRARFPA
                     ARVRRMDSDTMERQGAHEELLRALGAEEIDILVGTQMIAKGHDFPNVTLVGVVLADTA
                     LNLPDFRAAERTFQLLTQVSGRGGRGDHPGQVIIQTFNPGHYALQRAKDHDYEGFYRE
                     EAKLRRSLSYPPYARLIQLTLSCPHQTKGDQAAAALAALARKLLTAAEERGGPTVAGP
                     TEAPISRLRGRYRWQILLKGDDSRTVRKLARELLARFHVPGVRIKVDVDPVYFM"
     gene            complement(6491..6859)
                     /gene="dksA"
                     /locus_tag="PLT63_02400"
     CDS             complement(6491..6859)
                     /gene="dksA"
                     /locus_tag="PLT63_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HOD97600.1"
                     /translation="MPLKPEKLQFFRYMLNQKINGLLSDAEKTVSEMTTGKENYPDPN
                     DRASLESDRNFELRIRDRERKLIAKMREAIQRIDDGTYGICTACGCDISEKRLIARPV
                     TTQCIDCKTKQEKLEKMKGE"
     gene            complement(7108..8664)
                     /locus_tag="PLT63_02405"
     CDS             complement(7108..8664)
                     /locus_tag="PLT63_02405"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOD97601.1"
                     /translation="MGELILAIDNGTQSIRALIFDPRGNLRAKTRVMIEPYFSRQPGW
                     AEQDPDYFWQALCRACRQLWEETDIRPEALAGVALTTQRATVVNLDREGRPLRPAIVW
                     LDQRRTEGLEPLGGLWGMAFRLSGMRETVAYFQAEAEAVWLAHNQREIWKKTYKYLFL
                     SGYLTYRLTGEYRDSTSSQVGYIPFDYKRLRWAGPWDWKWKLLPVRKEMLPELVPPAA
                     EIGRITPRAAGETGLPAGLPVIAAAADKACEVIGAGCLEPHIGCLSYGTTATINTTHR
                     RYIEAIPLIPPYPAAVPGAYSLEIMIYRGYWMVSWFKREFAHNEEKLARERGLDPEVL
                     FDDLVRQVPPGSMGLILQPYWSPGVKIPGPEAKGAVIGFADVHDRAHLYRAILEGLAY
                     ALREGKERSEKRSGVPITELRVAGGGSQSDAALQLTADIFGIPAVRPHLYETSGLGAA
                     IDAAVGLKLHPDFTAAIAAMTRLGRVFEPHPDHREIYEQLYNRVYLQMYRRLKPLYEE
                     IRKITGADRR"
     gene            complement(8669..9145)
                     /gene="rfaE2"
                     /locus_tag="PLT63_02410"
     CDS             complement(8669..9145)
                     /gene="rfaE2"
                     /locus_tag="PLT63_02410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HOD97602.1"
                     /translation="MHKIVSKEKLKARLQKLRQEGKTIVFTNGCFDILHVGHVRYLRE
                     AKKLGDVLILALNSDTSVRAIKGEKRPLVPEDERADIMAALECIDFVVIFPETTPREL
                     IEYLEPHILVKGGDWREEAIAGADFVKGTGGRVVTIPLTEGRSTTNIVEKIRQVYG"
     assembly_gap    9446..9545
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9546..10202)
                     /locus_tag="PLT63_02415"
     CDS             complement(<9546..10202)
                     /locus_tag="PLT63_02415"
                     /inference="COORDINATES: protein motif:HMM:NF039185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOD97603.1"
                     /translation="MPRAFEVAQRLLRALADVLFPPRCQSCGRTADDLTVGLFCPACA
                     GEIRYITPPVCPACGAPGPTGQAADQLCGDCHRELKPFAVARSVAVYAGGLLRAIHDF
                     KYRRQPGLGRGLGRLITDNNYAGIDPEGFDVVIPVPLHVRRLRERGFNQSLLLARTVA
                     TACGIPVDFLSLRRVRDTPPQTLMGRKERQANIRGAFAVARRDRLRDRQVLLIDDVYT
                     T"
     gene            complement(10186..10626)
                     /gene="rsfS"
                     /locus_tag="PLT63_02420"
     CDS             complement(10186..10626)
                     /gene="rsfS"
                     /locus_tag="PLT63_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOD97604.1"
                     /translation="MLSSEKPTLPAANDAGAPPLSDSRERVLHCINTALSRKAQRLVV
                     MNVREISSFADYFLICSGSSDRQVKALCSHLQENLKKLGMRPLGVEGEKHGRWILMDY
                     GDVVVHIFLEPLREFYDLERLWVNAPRMAVPDETDRIEELAEGL"
     gene            10977..11795
                     /locus_tag="PLT63_02425"
     CDS             10977..11795
                     /locus_tag="PLT63_02425"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOD97605.1"
                     /translation="METIAELSVTRKVQAHLPFHFLYGQYLPKVLQYRLNPELSFNFH
                     TLDIFRENDYRRVAAPLREAGLRVTFHAPFMDLRPGAVDPWIRDASRRRIQQVIEVAA
                     YFQPAAIVCHPSFDDRYYVSSEDQWLENSIETWRRLLAAAAEIPVRMVLENVYERGPR
                     QLHRLLASLDTPQCGFCFDTGHFQVFAREPLTEWLETLYPYLCHVHLHDNNGDVDAHL
                     PVGEGKFPFADLFEFLEAKGCHPIFTIEAHSEQNLMRSLENLERLSFLGGDATC"
     gene            11789..12763
                     /locus_tag="PLT63_02430"
     CDS             11789..12763
                     /locus_tag="PLT63_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOD97606.1"
                     /translation="MLKYILKRLLFMIPMLIGITIICFAVMHLAPGSPTDLQTQMNPR
                     ASAELKERLRAMYDLDKPLYVQYGLWVQRLLILDLGKSFAPDRRPVAEKIGERLPVTV
                     TLNVLSLLLILAAAIPLGVLSAVHQDSWFDMAVSVFVFVGFAVPTFWLALLLMIFFGV
                     DLGWLPISGLRSLNYEYLPPGAAFWDLVKHLILPVGISAFGGLAGLSRYMRANMLEVI
                     RQDYIMTARAKGLSEFQVIYKHALRNALLPVITILGLSIPGLIGGSVIFETIFAIPGM
                     GQLFYMSVMARDYPTIMGILFIGAVLTLLGNLLADVAYAVADPRIRVG"
     gene            12773..13612
                     /locus_tag="PLT63_02435"
     CDS             12773..13612
                     /locus_tag="PLT63_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOD97607.1"
                     /translation="MGSEFQYRLAGNRMALAGGIIVILLFVVSLAAPLLAPYDPLAID
                     LTRILAPPSWQHWCGTDQLGRDVLSRMIWGAGISLKVGFVATGIAVLIGALLGALAGY
                     YGGWWDMVIMRLVDVMLCFPTFFLILAVIAFLEPSIWNIMIIIGATGWMGIARLVRAD
                     FMSLKERDFVTAARVLGASDTRIIFRHILPNAMTSILVAATLGVAGAILTESALSFLG
                     IGVQPPTPSWGNILTAGKDNIDIAWWLSLYPGLAILITVLGYNLLGEGIRDALDPRLR
                     GGR"
     gene            complement(13676..13906)
                     /locus_tag="PLT63_02440"
     CDS             complement(13676..13906)
                     /locus_tag="PLT63_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97608.1"
                     /translation="MKKLLSIVVSILFALSMSGLAFAQAAPAAKPAAEPVKAEEKAPA
                     KEKKAKKTKKAKKAKKAEKAEEKAPAAAAEKK"
     gene            complement(14110..14790)
                     /locus_tag="PLT63_02445"
     CDS             complement(14110..14790)
                     /locus_tag="PLT63_02445"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HOD97609.1"
                     /translation="MNKNTLLKILLFGLLFGGGTWLFIHYDLIQFFTNRNKAIDFINS
                     FGPLSVLVFIGLQILQVLFAPVPGEATGFIGGYIYGAVLGTIYSTIALIIGSWLAFSL
                     ARWLGLPFVEKAVRPTILQKYDYFMKHKGSWVVFILFLIPGFPKDALCYIIGLSHMPV
                     RMFLVVSTLGRLLGTAMLSISGDMLRNEQHGPLWILAAVSLLAVVAAYLYRDKLLNLL
                     KHKPPPLP"
     gene            complement(14893..15627)
                     /locus_tag="PLT63_02450"
     CDS             complement(14893..15627)
                     /locus_tag="PLT63_02450"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOD97610.1"
                     /translation="MYRVGTDRQQGLPEEFTIFAHRGASGYEPENTLRSFRRAMAMGA
                     RWLELDVYAVAGALVVIHDATLERTTNGRGYVMDQTLAYLRSLDAGKGEKIPLLEEVL
                     AMLGPGDGLNIELKGPATAVPLAALLAASPQGARTDPEQLLVSSFNYEELIALRRLRP
                     DLRIAPLVTAVPRGYARFAEEMGAYAVHGTLKAATEKFIADAHRRGLKFHAFTANTRK
                     DMDIMLARGADGVFTDYPDLRPGLCP"
     gene            complement(15756..16442)
                     /locus_tag="PLT63_02455"
     CDS             complement(15756..16442)
                     /locus_tag="PLT63_02455"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOD97611.1"
                     /translation="MAAYVLVHGATAGGWIWSPVPEMLRRQGHTVYTPTLTGLGERSH
                     LLRPDIDLDTHITDIVNVFKFEDLHDIILVGHSYGGAVIAGVAERMPERIRRLIYLDA
                     LIPEDGQAVKDLYPHDTAELLAAYIEARGEGWRYPPPKPRGDPRFVDHPARTMFQALT
                     RNNPQARAIPQAYIYCTDKSGAGPQHQGIIGVAARVRADGWPFFELATGHHPMQTAPE
                     DLVRILLSLL"
     gene            complement(16475..17281)
                     /locus_tag="PLT63_02460"
     CDS             complement(16475..17281)
                     /locus_tag="PLT63_02460"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOD97612.1"
                     /translation="MLEIKEYEDVIQIRMSREWEGKALYWVAAYLVDGLLVDTGCAHT
                     AAELVEFLAGKKLAMVVNTHFHEDHVGGNARIEKYFSVPVYAAPASISIIAGKPFLYP
                     YQEFVWGRPEPSPVLPVPAVITTPLYTFEVMATPGHADGHVALLEKNRGWCFSGDIFA
                     RQKPKFIRPEEDMGETIRSLERIMAAAPDKLVLFTSVGRIVENGRAALEENIRYLRDL
                     ARKARTLQQQGHSETEIVVELFGGEHLFAQMTDGQYTTANLIRSVLKMSH"
     gene            17665..18654
                     /locus_tag="PLT63_02465"
     CDS             17665..18654
                     /locus_tag="PLT63_02465"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HOD97613.1"
                     /translation="MLFKTAITEMFGIEYPVICGAMMWLCKPRFCAAVSNAGGMGNLT
                     AGNYETEEEFRAAIAETRRLTDKPFMVNITLLPSVRITPEHHKMYIRVCTEERVSGIE
                     FSGTPVDKVAGMAAIEDLKRAGVKLFHKVGSVRHALHAEKVGYDGIYAAGIEEGGHPL
                     DDDVTTMVLTPRIVDSVRIPVVTVGGVADGRALAAALVLGAQGVMMASRFVATRECEV
                     HENIKQELVRRQENETVIYGKSLGLQGRGLKTKVLEEVCAIEKRGGGFAELIPLLSGQ
                     RVKEAWETGDVDYAPLMVGQSIGFINDIPTCRELLERMGREAAACLDRAKKSL"
     gene            18673..18852
                     /locus_tag="PLT63_02470"
     CDS             18673..18852
                     /locus_tag="PLT63_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97614.1"
                     /translation="MAKQIYEGSMDKRILQMKIRAGELKEEDLRENLKNLPDVAANAE
                     PLTTVMERQDDGGAS"
     gene            18861..19643
                     /locus_tag="PLT63_02475"
     CDS             18861..19643
                     /locus_tag="PLT63_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOD97615.1"
                     /translation="MFIDSHAHLEMREFDRDREEVILRAAAAGVDYIVTVGTTLRECR
                     KAVEIARRHPAVYAAVGIHPHDAKEIDHNTYDVLKTLAGDEAVVAYGEIGLDFFRNLT
                     PPEIQRRRFAEQLEVAADLGLPVIIHDREAHGETVATLKAWRGRTGGVIHCFSGDTAM
                     AMTCVEMGFHISIAGPVTYAKSERLWEVVRQVPLERMLIETDAPYLTPHPRRGRRNEP
                     ACVVLTAQKVAELKGVSRDKVGRETSRNAMLLFDIPRKAKES"
     gene            19640..20530
                     /locus_tag="PLT63_02480"
     CDS             19640..20530
                     /locus_tag="PLT63_02480"
                     /inference="COORDINATES: protein motif:HMM:NF017868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HOD97616.1"
                     /translation="MTGELLYFAYGSNLNRDDLAKWCQEHRFDYPLGEKVANAYLPDA
                     RLIANYYSHFRRGGILNIKYQLGQVTPGALFRVLPGCWNVLDIKGDTENMYKHLNIVA
                     LTDDGREHVAVAYQIDASKTSPVFVPLHPDYRRIVREGMAAHGIDADVFTVVAEGRDP
                     DYPIKHIFVYGTLMSEGPRHYLLAAWADLESRLPARVHGLLYNSWKGFPCMIPDVTGR
                     SHVIGEIYTLTDLKKAFEILDIVETAKRYAATGTFFRRAIIEVTREDGSKCPAWTYFV
                     DMSVEGMPRIPSGSWRTVIA"
     regulatory      20630..20720
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            20801..21550
                     /locus_tag="PLT63_02485"
     CDS             20801..21550
                     /locus_tag="PLT63_02485"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HOD97617.1"
                     /translation="MTRDGVKRDTAALARATGNERRKGRGAMPGDGKKRIAVLAGSPI
                     GDLEWLRRELAAAAPEAVLCADGGARYARLLGVVPDLIVGDLDSLTEELQRAFRDEGV
                     AMDVYPAGKDETDTQLALERALAMGAGEILVFGALGNRLDHTLANIGLLLLGRNRGAA
                     VILRDESCDVFAVTGESVIRGEAGQTVSVFPLDGDAAGIDLAGFEYPLDGAAMTLSRP
                     YGISNRLAAPTGRIRVGEGCLLVIHYRRAVA"
     gene            21587..22399
                     /gene="thiD"
                     /locus_tag="PLT63_02490"
     CDS             21587..22399
                     /gene="thiD"
                     /locus_tag="PLT63_02490"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HOD97618.1"
                     /translation="MKGIKRILTIAGSDSGGGAGIQADLKTITVLGGFGMSVITALTA
                     QNTLGVHAVHEVPVEFIAAQFDAVVSDIGVDAAKTGMLSSAAVVEVVVEKIGQYGINK
                     LVVDPVMTAKGGATLMEPGARKAVMAKLLPLALVVTPNIPEAEILTDVIIASVSDMKA
                     AARIIHELGAQNVIVKGGHLIGAAADVLYDGHDFYEFPFVRVATKDTHGTGCTFSAAL
                     ATALAQGRSVREAMAAVEDYMAAVIGNSLRLGAGHGPVNHWVCPAPSGGTER"
     gene            22595..23896
                     /gene="thiC"
                     /locus_tag="PLT63_02495"
     CDS             22595..23896
                     /gene="thiC"
                     /locus_tag="PLT63_02495"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HOD97619.1"
                     /translation="MTQIEQARQGMITEETTISAAREGCPPEYIRAGVAAGTIVVIRN
                     KVHGAIAPLAIGKGLRTKINANIGTSKDRADLVDELEKVRICVEAGADTIMDLSTGGD
                     IQAIRRAIVAASPLVIGTVPIYQAAARVLARGKAIVEMTAEDMFEVIEENGADGVDFI
                     TVHCGVTRRSVAAVEAQGRILGIVSRGGSITAHWMECNGEENPLYAQYDRLLDIARKY
                     EIVLSLGDGLRPGCLADATDRGQIQELILLGELAKRARDRGVQVMIEGPGHVPLTDIE
                     SNILLQKRICAGAPFYVLGPLPTDIAPGYDHLTSAIGGAIAGAAGADYLCYVTPAEHL
                     RLPTLEDVREGIIAARIAAHIADIAKGLPGAAERDRHMAVCRKEFDWQGQVEASLDPE
                     RTGAFLEKSASARDEGCTMCGEFCAIKLGRMAKDRATGAKC"
     gene            23964..24164
                     /locus_tag="PLT63_02500"
     CDS             23964..24164
                     /locus_tag="PLT63_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97620.1"
                     /translation="MMQVLWTFVIVSVVLLAFFFLRPPWSLENIIIGGVPLVLLVIFA
                     CRLRQAKKERAAAPPEDDDEEG"
     gene            24175..25554
                     /locus_tag="PLT63_02505"
     CDS             24175..25554
                     /locus_tag="PLT63_02505"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HOD97621.1"
                     /translation="MVLPLTVNGAASKRDGAVSIARALRGAGHEAFFVGGCVRDLLRG
                     ETPDDYDIVTSARPEQVMAVFPRTYPVGAAFGVVLVREGEHAYEVAAYRKEADYEDGR
                     HPSRVSFVGAREDVCRRDFTVNGLLMDPETGEIVDYVGGRADLEKGLIRTIGAPEERF
                     AEDHLRMLRAVRFAANLGFRVEDAALAAIIHQADKILRVSAERVREELNRILAGPRPR
                     RGMELLASSGLLSRLLPELVALRGVEQPSRFHPEGDVWEHTLRMLALLPAENLPGEEA
                     ERLAWAILFHDAGKTATRTEDETGIHFYGHVSKGVEIAAAVLKRLKFSREDGEVVLAL
                     VANHMRFMHVRQMRVHTLKKFLRLPDFHLHLILHRLDCLGSHGLLDNEAFCRERLGEM
                     TGEDLHPSRLLTGDDLLALGFSPGPVFREILTMIEDAQLRGEIATPAEAHALVRRKFF
                     PTAGAGGRG"
     gene            complement(25508..25915)
                     /locus_tag="PLT63_02510"
     CDS             complement(25508..25915)
                     /locus_tag="PLT63_02510"
                     /inference="COORDINATES: protein motif:HMM:NF023514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3568 family protein"
                     /protein_id="HOD97622.1"
                     /translation="MKNNFIAVLSILILTVLAGCEGGLVLGGKSVAVQDGQVVQSSGY
                     VVSSYPWPFETVCRALDDTLKEMQAVVNEKTVKIGKARVLAVIQGEKVTFEVEFKDKD
                     KTDVSILVGLGGSNIASRLIHDRLRRQLEKTSS"
     gene            complement(26014..26871)
                     /locus_tag="PLT63_02515"
     CDS             complement(26014..26871)
                     /locus_tag="PLT63_02515"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOD97623.1"
                     /translation="MTAERTSGLCPNCRKLISLDEKSCPYCGLANPGSRRMRFFLSRW
                     AGGNGDLVRIIIYTNVAFYILSLLMNPRGMGMTMNPLMLLSPSEGSLFLLGATGTLPI
                     GHFHRWWTLITASFLHGGLLHIFFNMMALNQLGPFVVSEFGVHRFAVIYTLTGIAGFL
                     LSFFAGIPFTIGASASICGLIGAILYFARSRGGYFGMTMYRQAMGWVVGLVIFGLLVP
                     GINNWAHGGGLAAGVAAAFILGYEERQRETAGHRVLGKACIYGTAILLVWAVFQAFSQ
                     LLQHAAYFR"
     gene            complement(26873..28042)
                     /locus_tag="PLT63_02520"
     CDS             complement(26873..28042)
                     /locus_tag="PLT63_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HOD97624.1"
                     /translation="MDHPNIHRLGWEGKELVLVGTAHVSRESAELAEAVIAAEHPDAV
                     CVELCNSRYETIKQREKWQEMDIVKVIREKRTSVLLFQLLLASFQKKIAEKLGITPGE
                     EMIRAVNRAEALGIAVVLADREIRVTLLRAWRKMGFWTKIRVLPEMIVSLFYTEDITA
                     EEVEKLKEQDVLEVALKTFGEKLPTLKATLIDERDAYLSHTISHADGRKIVAIVGAGH
                     IPGIKRNLGRSVDMDAISAVPPPGIWGRYAGWVFSAAIVVLFIAGFFYSGGRTSMHMV
                     ASWSAITAACAGLGALIMLSHPLTILAAAFSAPIATIHPLVATGWVAGLTEATLRKPQ
                     VRDFLELKDDISSVRGFFRNKITRLLILVAFVNLTTMAGVLVAIPVIMKYFFTGG"
     gene            28514..31546
                     /locus_tag="PLT63_02525"
     CDS             28514..31546
                     /locus_tag="PLT63_02525"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOD97625.1"
                     /translation="MNKLMLWSLRNAKLVIIINVAITIVFGWFALQIKVDSSTEGMMI
                     TGDPAKDYHQDTVRKFGSDNITVVFVRDKNLFSPEKLKDVEKLYYELKDIKFEPKDPE
                     EYVRAKDPEEYLKIRYPAVYEKISAHAEEYAKLTKSREEYNKLVYEPETYLKHKFPDT
                     YKELLKGKTQPSKEELKQQFVEAVERIGKPGVGRCESLFSVTNFKGVSGALETNPLMD
                     RAPETQADADQIKADALKSPLFMNSLIDKEGQMTAINLYVDVDPTEPEFHTYFSRKVD
                     EISGKYENLFDRVFQFGNSYGRRNIGENIIKDQSRIVPLACAVLIGTIIIALRNVGAG
                     LMPMITAGTSVIWTFGFMVVAGIPLNVITVIIPSILLVIGSAEDLHMMHEYLEGIEET
                     NGNRDGSIKLMASKLGLAVLMTSITTIVGFASIMYNDISMLIQFGQVATFALTANSLS
                     TFTFGPALLHLFGPVKVRKHEEKKHFIDAFFHGIAVGTLKLVTPKKNKIIISVVMILL
                     TLGMFGSTFFVKVNNDFMGYFKADSEIRKRSDTLHKELAGAQTFFIRINSGMPETFKQ
                     SEYLSQVAALQSYMAQKGWFDKTESLADRVALIHREMNNGDMKFYAIPADSNLISQYL
                     LFFQRDEISRFTTPDFSEVSIMVRHNVTSSYEIQQILEELRQYMSKNINPNFKYEFTG
                     EYILINKAAESLVLNSVTSLALTLFIVFICIYFLFWSMKAGFISLVPNVFPIVGIFGI
                     MGLFDVPLNVGTAMVADIAIGIAVDDTIHFMSRYNQAMRELQNREAAVAVAVHDEVRP
                     VVCSSVALAGGFAICAVSQFIPVIQFGILSSVVMIIAIFSELLITPILLQSTQLITLW
                     DMIGLKLQDAVVKTSPFFEGLRPWSRRKVCLLGRMVEKNVGEKAVVQGEFGKSMYLLL
                     EGKAEAVGVDAKTGKEIVFASLHPGDIFGEIALVNPGPRSADVRAVEPVKYLEIDWEG
                     LKRIQKIYPRIGGQLFLNLSRILGEKLVHTNEMLFGKA"
     gene            31836..33446
                     /locus_tag="PLT63_02530"
     CDS             31836..33446
                     /locus_tag="PLT63_02530"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HOD97626.1"
                     /translation="MIESFLLLSSGITLFLFGMMKLGAGIQQLFTARIRDHIKYAVRR
                     PFFGLLTGVAATVLFQSSSATTVIAVGMVSAGLITFYHSLAIVLGADLGTTVIVQLVV
                     WKITDASPLFVIVGGIIWFTAQERRKSLGEAIFYFGLLFFGLSVVGTATAPLQQHPEI
                     VGFFREAKHPLLGFTLSALFTGLIHASAIPIGILAILAQQDLVSLDNALPMVIGANVG
                     TTVTALLAGMVSGVGGKRAAFSHFFFKAVGAAICMAALPPFLALLRSLSPGVAQQIAL
                     GHLLFNLIIVVLFFPLLRLVARLLVRILPGEEKTLPLWPEFLKEDALADPVSALEIVS
                     RELERQIVIVEELYRRATPLRTAYREGQRRDVLYVEMIINHLRAEIVSYLRKISCQDL
                     SPALSGRLFAYTAMADDIERMANHMINLVSLSREKQRRRIEFTRFAEEELDEIERLVE
                     MNIADAVRLIRQGDGDRSAAVTRRENEIDDAVKAARERHLVRFHKRLCQAEAGPVFVE
                     MLIHLERISDHCQNIADHVAELEAAEVS"
     gene            33539..34057
                     /locus_tag="PLT63_02535"
     CDS             33539..34057
                     /locus_tag="PLT63_02535"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOD97627.1"
                     /translation="MKDRLNALEVALNNELREHEFYLKNAERTKNPVGKAMFLQIAAE
                     ELEHYERLQQLHEKWDKEGKWPETVPLKVKETKVRDILKKVVQEAGRGAAGDADDRKA
                     LETAIDFEGQGAAYYARLRDSVKDPREKAFFELLANIEHEHYVSLKDTEEYLLDPAAW
                     YRNRERGGLDGA"
     gene            complement(34066..34899)
                     /gene="budA"
                     /locus_tag="PLT63_02540"
     CDS             complement(34066..34899)
                     /gene="budA"
                     /locus_tag="PLT63_02540"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HOD97628.1"
                     /translation="MPDQQIPAIEMEITLPADLVGELKRAAAAVGHTTTEITRAALAH
                     YLEARRRSGEEAVSAVYLSAPVTAMLKGFYEENTLLGDLKRHGDFGLGTFNNLDGEMV
                     MLDGIIYRLRADGKTYPVADEVQTPFACVTFYRPTVSEEFAVELDYAAFQALLGRLLP
                     SDNMLYAIRVDGFFQEVQVWSVSRQENYQPLNEEDHNRPALSFENIHGVLAGFYTPKF
                     IRSLNFPGFHLHFLTADRRYGGHLHACRTGRIEIGIQFIQQLKLDLPMTIDYLTAHLP
                     R"
     gene            complement(34989..>35956)
                     /locus_tag="PLT63_02545"
     CDS             complement(34989..>35956)
                     /locus_tag="PLT63_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOD97629.1"
                     /translation="LNVQMAFFSQESAENLRYDRTVWEEALDVPSHSHDQERRNLLGA
                     FLFSGDDIHKPVAVLSGGEKSRLALLKILLRKTNLLILDEPTNHLDLKTKDIFQSALL
                     AYPGTVVLVSHDRYFLDRLVNRVFELRDGACREYLGNYSDFIEKRQSSRDVSAMGKTP
                     EAGAEGRDRKGVTLSGRTSGEANASVTDAKRAISATAGAEEAAGEVRGNRFVGCTGEF
                     PGEVPGGAGRAVRTREERRREAEERNSLARQRRALRKDLQAVEADITDREQRKAAGEE
                     FLCHPFSHREPERIKQTVRDLRTLEKEIEALYDRWHELAASLEGV"
     assembly_gap    35957..36056
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<36057..>37273)
                     /locus_tag="PLT63_02550"
     CDS             complement(<36057..>37273)
                     /locus_tag="PLT63_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOD97630.1"
                     /translation="DLSWHITERSRIGLVGDNGAGKTTLLRAILGRVELDGGAIEITD
                     RRNRSLAYLPQDLEELPDVPLMDYLRDRCGIAALEKTLRVCEGQIAAAVCCLPGAAAG
                     ATGETESQEEDETTASYRRLLKDYETALALFNARDGYSFEARAGQILKGFGFREADHH
                     RNCGEFSGGWKMRILLAVILLSRPDIMLLDEPTNHLDTESMEWLESYLKDYPGTLITV
                     AHDRFFLDKMATTIAEIGRGRLIIYKGNYSWYLREKERRRAALEKERHLQHSEIKRTE
                     AFIERFRYKATKAKQVQSRLRLLEKFSPLEAGESEQAVRIKFPAGTKSGKEVLTVRDL
                     GKEYGGIPVFSGVGFTLHRGERVALVGVNGAGKSTLSRLLGGVEAPSAGEIRYGLNVQ
                     MAFFSQESAENLR"
BASE COUNT         6992 a        11205 c        11560 g         7316 t
ORIGIN      
        1 gacaaaaaga aggccagcct ttgatgacgg catcgtaaaa agtccggatg cttcgaaagc
       61 gcttcatccc tcgtcactgc gacgtacgtc caagtacgcc tcattcctca ggattcgcgc
      121 gcctcgcctg cggatctttt tacgatgccg tcccaaatgg gacatttttt gcgcttttta
      181 cgatgtcatc tttgatgggc atccataaaa aaggatcagt tcagacgaca aaagaggcaa
      241 attattcata acgataataa aggcggggga tttcaaccaa ccccgcagag ggcgtcaatc
      301 atcccgcctt gtcaaatttt ctaattatct gtggccggct cgttttccgt ttgcataagg
      361 acaagcagac gattaagcag ccaaatgcac ttttccatca tcacggtcag gtccaggagg
      421 catcctttct cctttgccga accgagtttg tcgctgtcca tataggcctt gcgcaactgt
      481 tccagcatcg ggccgctgct ggaactgagg tccaggagca gccgtacctg ctcgggggag
      541 tttgttttta ccgtatcggc ggcataattc aacaggaagt ccatcgcttc cacgcaaacg
      601 tccgtgaact ccaccagaga aggatacctt ctgggccgcc ggatcgggca gttttggtaa
      661 tcccggcagg tatggcattc cggggagaga ttttatgtag aggtcccagg ttttcgaaaa
      721 gctgccgaag aggtcgctgg taaagagcgc tttcgttttt gtgtcgtagg ttatgatcct
      781 cccgccggca tggttccggg cagcccccat atgccgccta aaattccatg tgggcaagtt
      841 gccaggaaag accgcggcgc ctcagaatga accgcaccgc ctccccttct tcccccgccg
      901 gcatggtgac ggtgaactcg gagggagagg tgtaagcgaa atcggcgtgg cgcaggaagg
      961 ccatgaacaa agcccgggcg ttgggccggt ccaggccgcc ggccgcagcc agggaggcca
     1021 ggcgctcttt catgtaggcg gccagtcctt ccggggtgat caggtcgtcc acagcccggt
     1081 ccaccatccg ggcgaccagg gaatcacgga ggtcccgggc ggccgccggc gggtcgtccg
     1141 ccgggtcgcc gaggatcttg gcgcccagat aacgcttgag attctctctg acttcggaaa
     1201 aatcgaccag agcggccagc ttttcaatat cctggttttc gaatgccgcc atgatccgat
     1261 agatcgccac atagggcagg ctgtacagcg cccccgctcc cagggacaag aggacgcagc
     1321 tggtgattac tatcgccttc ttcttcaagg tcgttactcc cagccgatga cttcgtagcc
     1381 cttttcaccc aggcagcggt tgacgaaacc tttataaacg gcacttggtt ccgaggcctt
     1441 gatcaccccg gtaaccagac cggcggcggc ccccgccgcg gcccccacgc ctaggtccga
     1501 cccgaagctg ccccgggccg cgccgatggc cccgccgacc accgccccgc cggccccgcc
     1561 gatggccgta tccttggcga ccgtcttggc ggcgtcggac ttcacgtaaa cgtccgcctg
     1621 ctgccggcag tcggcaatat cccgctccgc ctgctcctgg ccgaccttgt tcaggtgggc
     1681 gttggggtac aggaccggat tccaggcaca gccgccgagg agcagaatta agcctattgt
     1741 taaataactc attgttttca tttgtattta tcctctgttg tgttgatgct catcccatcc
     1801 cggtttcctt ttccagcacc gccccaaaaa aaccgtcagt gccgtgacgg tgggggaagg
     1861 tgcggaggta tccgtcgccg tccaggagtt ccgacgccgg ggcttccgcc gagattctgt
     1921 ggaaatcccg gcgttgttcg agaaaagccc ttaccacgtc ctcgttttcc tcggccatga
     1981 tgctgcaggt gctgtaaacg agccgcccgc ccggtttaac gtagtcaccg gcccgggcca
     2041 gcaatctttt ctgcagggcg gcgtgacgcg ccaaccggcc gggcgccaga cgccacttga
     2101 tctcggggca acgccgcagg gtccccagcc ccgagcaggg gacgtccagg aggacccggt
     2161 cgcaggaacc aggcgggacc tccacgaggg gatgggtggc gtcggcggcg acggtggaga
     2221 tgacacttat gcccaggcgg gccgccaaac catgcaactt cttgatttta ctcgtaagaa
     2281 gctccaccgc gatgatccgg ccccggttat ccgtcagttc cgccagatgg gaggccttcc
     2341 cgccgatccc ggcgcagaga tccaaaatcg cctcgccggc ggcgggatgg accagccggg
     2401 cgatgagctg agaggcctcg tcctggatct gaaagtctcc ccgccggtag gagagggtat
     2461 cccggaggag ttttcccgcg gaccggacca ccaatccctc gggggcgtaa cgggttttct
     2521 ccgcaacaac tccttccccg gccagtgctt ctataacctt tttcccggtg gtttgcaacc
     2581 ggtttaccct caggaccgta tccgggatgg tgttgttggc tcggcagagg gccagggttt
     2641 cctccgggcc ccattgccgt agccaccgtt ccacgagcca gggggggtgg gaatggagga
     2701 cggagatata ccccggtaaa tccacgcggc gctccggata gggcaggcgg tcccggcggc
     2761 gcaggacgcc tctcaggacc gcgttgacca gggcctcccg gccggggcaa agggttctgg
     2821 ccagggcgac cgcttcattg acggcggcgt ggggggggat ccgttccgta taaaagagct
     2881 ggtagagggc ggtgcggagg atgcttcgta gggacgtttc ctccggggcg aggtccttgc
     2941 ggagaaactg cgccaggacc caatccagga agccccgcat ccgcagggtt ccgtaaacca
     3001 actccgtgac cagggcccgg tcccggggat cgccgtctcg ccaccgggag aggcagcggt
     3061 cgagaagcgg ctcggcgaag gccccggatg tgtcgatgcg gttcaggatc atgacggcga
     3121 gacggcgggg ttgctgggga accatgacga tcctgcctat cctatccttt ctgtccgcgg
     3181 cgccgcttac ccgcaacggg accgttcggg aaggcggaaa ccccgcaaaa agtcgtggat
     3241 cgccatacgg ttcttgcctt ccagttgcac gtcctggaga taaacccagc catcgtccgc
     3301 ggccacgggg agcccatcct tcgtcgccgt cccgacggtt cccggcgccg ccccggtgtc
     3361 ccccggtctc ccggccgctc gatagatctt cagcatcttg ccgcccagaa aggtataggc
     3421 tcccggcgtg gaagagaggc cccggatcag attcacgatg gccccggcat cccgacgcca
     3481 gtcgatgcag ccgtcttcct tcctaatgcg gggcgcgtag gtggcctcct cttggcgctg
     3541 gggtgtccgg accgcccgtc ccgccgccag cagggccacg gtctccagga ggagctccgc
     3601 ccccagccgg gccaggcggt cgtgaaggag gtcgtaagtc tctccgggtg ccaccggcgt
     3661 ttccctccgc atgatgatgt cgcccgtatc gacgccggcg tccatcatca tgatcgtcac
     3721 ccccgtggtc gtttcccccc ggatcagggt ccagttgagg ggcgcggcgc cccggtactt
     3781 cggcagcagg gatggatgga cgttgatgca acccaggggc ggtcgttcca ggatctccgg
     3841 gggcaggatc tgaccgaagg cggcgaccac caccaggtcg ggattgagtc cccggaacaa
     3901 ctggagaaag gattcgtccc ggactcgctc cggctggagg atcagcagtt cgtgggccgc
     3961 ggccagttcc tttgccggcg gcggttgcag gcgccgcccc cgcccccggg gtttgtccgg
     4021 ctgggtgatg acccccacga tgggataaac ggcctcgatg aggcgccgca gggacggtac
     4081 ggcgaattcc ggagttccca tgaacaacaa gcgtggtttg gacatgatga gcggaccttc
     4141 cttattttta tgtcggcggc ttctccggcg gtatttatag gaaatctctg caaaggaaaa
     4201 gcaaaatctt tcggggcgct cacatgaaat ataccggatc cacatccacc ttgatccgta
     4261 ccccgggaac gtgaaaccgg gccaggagtt cccgggccag cttccgcact gtgcggctgt
     4321 cgtcgccctt gaggaggatc tgccagcgat agcgcccccg gagccgggag atgggggctt
     4381 ccgtcggccc cgccactgtt ggcccgccgc gctcttcggc cgccgtcagg agttttcggg
     4441 ccagggcggc gagggcggcg gccgcctgat cccccttggt ctggtggggg caggacaggg
     4501 ttagctggat cagccgggcg taggggggat aggagaggct tcggcgcagt ttcgcctcct
     4561 cccggtaaaa accctcgtaa tcgtgatctt ttgccctttg gagggcgtag tggccggggt
     4621 tgaaggtctg gatgatcacc tgtcccggat ggtcgccccg tccgccccgg ccggagacct
     4681 gggtcaggag ttgaaaggtc cgctcggcgg cgcgaaagtc cgggagattc agggcggtgt
     4741 cggcaaggac gacgcccacg agggtgacgt tgggaaagtc gtgccccttg gcgatcatct
     4801 gggtgcccac aaggatgtcg atctcttcgg cgccgagggc ccggagcagc tcctcgtgcg
     4861 ctccctgccg ttccatggtg tcgctgtcca tgcgccggac ccgggccgcg gggaaccggg
     4921 cccggatttc atcctccaga cgttccgtcc ccacgccgta agtcccaatc cgcgggcttt
     4981 tacagtggga gcagacggac tgtcccgtga cggaataatc acagtagtgg catttccacc
     5041 ggttgtcgcc ggcatgatga atcagggaga cctcgcagtt ccggcagcgg aggggacggc
     5101 cgcaatcacg gcagaccagg aaggtatgat agcccctccg gttgaggagg agcagcgcct
     5161 gttgtcgccg ctggagagtg acgtcgatgg cggcgagaag gcgccgggag agaatggcgc
     5221 cggccccttc ctcccgaatg gccttttcca tggtcatatc gacgagctcc acggcgggca
     5281 gcggccggtc ggcgatgcgc tgcggcatga ccagcaagga gactttgccg cgggtggtgt
     5341 tgaaataggt ctgcagagcc ggggtggccg aaccgaggag caccgtagcc ccctccagtc
     5401 gcccccggac gatcgccatg tcccgagcat ggtagggcag tcgttcatcc tgtttgtagg
     5461 aggcgtcgtg ttcctcgtcc acgacgatca acttaaggtt gcgaaccggg gcgaagaggg
     5521 cggagcgggc tcccacggcg atggatagag tgccggactc cagaagccgc cactggtcat
     5581 agcgcacggc gcgggagacc ccgctgtgga gtacggcgat gttgtccccg ccgaatctcc
     5641 cttccaggcg ggtaagcaac tggggggtga gggagatttc cgggaccaga tagatcacgc
     5701 ccccgccgta gcggcgggtt tcctccatgg cccggaggta aacctccgtc ttgccgctgc
     5761 ccgtcacgcc gtggaggagg aaggtttgga aatgtccgcg gcggaggctg gtcacgatct
     5821 cccggagggc cgccttttgt ccggcgttgg gtacgggccg gtccgccttc ggccccactt
     5881 gccggcggcg cggcggggga cggaacgtct cccgttcgct gatctccacc agaccctttt
     5941 tcaccaaggc ggccaggacg ggccggacat cccccagatt gcggcggagc gtcgcgaggg
     6001 aaacctcgtc gcttgcgccc tcctcaggga tcgcctctag gacgaggcgc tgccgggggg
     6061 tcagggccgg gacggcggcg gtttgtatcc ggagcaccgt tttttccttt ttcgaccgga
     6121 tgttcttgcc tcccgggagg atttcgtgca gggccttccc taagggatag agataatagt
     6181 ctgctatcca gcggtaaaag gcgagttccc gggcgccgaa caggggcctt gtatcgatga
     6241 tttcagtgat ttccttgaga ttttccacat ccgttgtggc ggagatggca atgatgcagc
     6301 cggtttgcgg tttgccgccg aagggcgccc ggaccctttt ccccacggca acggcggcga
     6361 cctgggaggc gggcacggca taatcgaaga cctgctcggc ggagatggga aaggctactt
     6421 tgacgaacat ggacgtcccc tcattcggat cgcggccgac gccggttcga aaacgaggga
     6481 agaagggatt ttattcaccc ttcatctttt cgagtttttc ctgtttggtc ttgcagtcga
     6541 tgcactgggt ggtcacgggc ctggcgatca gtctcttttc ggaaatgtcg caaccgcagg
     6601 cggtgcagat tccgtacgtg ccgtcgtcta tcctctggat ggcttcccgc atcttggcga
     6661 tcagttttcg ctcccgatcc cggatgcgca actcgaagtt gcggtccgat tccagggagg
     6721 cccggtcatt gggatcggga tagttctcct tgccggtcgt catttccgaa acggtctttt
     6781 ccgcgtctga aagcagccca ttgatcttct gattcagcat gtaccggaaa aattgaagtt
     6841 tttcaggctt taacggcatc acatcaccta aatcttttat tatcaacctg atacgtatag
     6901 gtgagacctc atacacgatc acgggatgtt tgtaaaggaa aaaaaccggc ggacgatgaa
     6961 cacgccgggc ggaagtcgcc ggttatgccg gttcctgatt tacccccggc cggggggagg
     7021 aataacgaac atcctgttta gagcggccgg atttcgatcc tcggttctcc gggcggcccc
     7081 gctgccgccg tgacgtggcc gggaccttca gcggcggtcg gcccccgtga tcttccggat
     7141 ctcctcgtac aggggtttga ggcgccggta catctggaga taaacccggt tatagagctg
     7201 ctcgtagatt tcgcgatggt cggggtgggg ctcgaatacc cggcccaggc gggtcatggc
     7261 ggcaatggcg gcggtgaagt cgggatggag cttgagaccc acggcggcat ctatcgcggc
     7321 ccccagcccg gaggtttcgt aaaggtgggg gcggacggcg ggaatgccga agatgtccgc
     7381 cgtaagctgg agggcggcat cgctctggga accgcccccg gcgacccgga gctccgtgat
     7441 ggggacgccg ctccgtttct ccgagcgttc cttgccctcc ctgagggcat atgccagtcc
     7501 ctccagaatg gcccggtaga gatgggcccg gtcgtggacg tcggcgaagc cgatgacggc
     7561 ccccttggct tccggtccgg gtatcttcac ccccggcgac cagtagggct ggaggatcag
     7621 ccccatggaa cccggcggga cctgccggac caggtcgtcg aagagtactt cggggtccag
     7681 gccccgctcc cgggcgagct tctcttcgtt gtgggcaaac tcccgcttga accagcttac
     7741 catccagtag ccccggtaga tcatgatctc caggctgtag gccccgggca cggcggcagg
     7801 atacgggggg atcaggggga tggcctcgat atagcgccgg tgggtggtgt tgatagtggc
     7861 cgtggttccg taactgagac agccgatatg gggctccagg cagccggccc cgatgacctc
     7921 gcaggccttg tccgccgcgg cggcgatgac gggaaggccg gcgggcagcc ccgtctcccc
     7981 ggcggcccgg ggggtgatgc ggccgatttc cgctgccggc gggacgagct cggggagcat
     8041 ctccttccgt accggcagga gcttccattt ccagtcccag ggtccggccc agcggagacg
     8101 cttatagtcg aacgggatgt accctacctg gctgctggtg gaatcgcgat attccccggt
     8161 aagacggtag gtgagatagc cggaaaggaa gagatacttg taggtttttt tccagatctc
     8221 ccgctggttg tgggccagcc acacggcctc cgcctcggcc tggaagtagg cgacggtctc
     8281 ccgcatgccg gagagacgga aggccattcc ccagagcccg cccaggggtt ccagcccctc
     8341 ggtgcgccgc tggtcgagcc agacgatggc ggggcgtagg ggtcgaccct cccggtcgag
     8401 gttgacgacg gtggcccgct gggtcgtcag ggcaacgccg gccagggctt cgggacggat
     8461 gtccgtttcc tcccagagct gccggcaggc ccggcagaga gcctgccaga agtagtccgg
     8521 gtcctgttcc gcccaacccg gctgccggga aaaatagggt tcgatcatca ccctggtttt
     8581 ggcccggaga ttcccccggg gatcgaagat cagggcccgg atgctctggg tgccgttgtc
     8641 gatggccagg atcagttcac ccatgacatc agccgtaaac ctgccggatt ttttccacga
     8701 tgttggtggt cgatctgccc tccgtgagag ggatggtcac cacccggccg ccggtcccct
     8761 tgacgaagtc cgccccggcg atggcctctt cccgccagtc gccgcccttg acgagaatgt
     8821 ggggctccag atattcgatc aattcccggg gggtcgtctc cgggaagatc acgacaaagt
     8881 cgatgcattc cagggccgcc atgatatcgg cccgctcgtc ctccgggacc aggggacgct
     8941 tctccccctt gatggcccgg acggaggtgt cactgttcag ggccaggatc agcacgtccc
     9001 cgagcttctt cgcctcccgg agatagcgga cgtgccccac atggaggata tcaaagcagc
     9061 cattcgtgaa aacaatggtt tttccctcct gtcgcagctt ttggagtcgt gctttcagtt
     9121 tttccttgga gacgatttta tgcatggctt gccctcataa gcggccgcgg tgcttgcata
     9181 actgatttcc gcggctaagg tctttttttg ccgtccggca tgtcgcgttg ccggtccgcc
     9241 aaacatggaa agcgatcccc gggcggatag accccgctcc gttcatggcg ggtcgtcggc
     9301 ttccgggacc gcccgggcca gggtcaggac gcctacccgg gcggcgccgc cgtccagaag
     9361 cgcccgggtg cactccgcca aagtgcttcc cgtggtgtaa acgtcgtcga tgagcagaac
     9421 ctgccgatcc cggagccggt cccggnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9541 nnnnncgtgg tgtaaacgtc gtcgatgagc agaacctgcc gatcccggag ccggtcccgg
     9601 cgggcgacgg cgaaggcccc ccgaatattg gcctgccgtt cttttcgacc catgagggtc
     9661 tggggcggcg tgtcccgcac gcgccggagg ctgaggaaat ccaccgggat accgcaggcc
     9721 gtggccaccg tccgggccaa gagcagggat tggttgaaac cccgttcccg gagccgccgg
     9781 acatggaggg ggaccgggat gacgacgtcg aatccctccg gatctatccc ggcgtaattg
     9841 ttgtcggtta tcagccgccc cagtccccgc cccagtcccg gttgccggcg gtatttgaag
     9901 tcatggatcg cccgcagcag gccgccggcg taaacggcca cggaccgggc cacggcgaag
     9961 ggcttgagtt cccggtggca gtccccgcag agttgatccg ccgcttgccc ggtcggtccg
    10021 ggggcgccgc aggccggaca aaccggcggg gtgatatagc ggatctcccc ggcgcaggct
    10081 gggcaaaaga gcccgacggt cagatcgtcc gccgtccgcc cgcaggattg gcaccggggc
    10141 ggaaaaagga cgtcggctaa cgcccgcagg agacgctgcg ccacctcaaa ggccctcggc
    10201 aagttcctcg atccggtcgg tttcatccgg cacggccatc cggggggcgt tcacccagag
    10261 gcgctccagg tcgtaaaatt cccgcagggg ctccagaaag atatggacga ccacatcgcc
    10321 gtagtccata agaatccagc ggccgtgttt ttccccctcc accccgaggg gccgcatacc
    10381 cagtttcttg agattctcct gtagatggga acacagcgcc ttcacctggc ggtccgagga
    10441 accgctgcag atgaggaaat aatcggcgaa agaggatatt tcccggacat tcatgacgac
    10501 cagtcgctgg gctttgcggg acaaagcggt attgatgcaa tgcaaaaccc tttcccggga
    10561 gtccgacaag ggcggggcgc cggcgtcgtt tgccgccggc agggttggtt tttcggagct
    10621 gagcaaagag cccccttccg tataaagtgt attaacctgt tgttttcagt agcagattaa
    10681 gctatccctg tcaacataaa atacatccgg cacaggtgca tgccgccgcg gggctccgga
    10741 gccaattctt gattgtcttg agcgcctttc ctatggtaaa accgcaagca tcgccaacag
    10801 accctgtgcg gcgccgtgct tcaaccttca tcactacggc gtgcgcacgc acgcaccgcg
    10861 tttgtcagga tgcgcacacc ctgcctccgg agctttttgc ttcgccgtct cggtcaagac
    10921 tttttatgga ggcagcgtaa catatcacca tgagcttcgc gtggttagga ggtgaaatgg
    10981 aaacaattgc cgaattgtcc gtgaccagga aggtgcaggc ccatctgccc tttcatttcc
    11041 tttacgggca gtatctgccc aaggtcctgc aatatcgcct caatcccgaa ctgagtttca
    11101 acttccatac cttggatatc tttcgggaaa atgattaccg ccgggtggcc gccccactcc
    11161 gggaggcggg cctgcgggtg acctttcacg cccccttcat ggacctgcgt cccggggcgg
    11221 tcgatccctg gatccgggac gcttcacgac ggcggataca gcaggtgatc gaggtggcgg
    11281 cgtacttcca gcccgccgcc atcgtctgcc acccctcctt cgatgatcgt tactatgtct
    11341 cgtcggaaga ccagtggctg gaaaacagca ttgagacctg gcggcggctt ctggcggcgg
    11401 cggcggagat ccccgtccgg atggtcctgg aaaacgtcta cgagcgggga ccgcggcagt
    11461 tgcaccgcct gctggcgtcc ctggatacgc cccagtgcgg gttctgcttc gataccggtc
    11521 acttccaggt ctttgcccgg gaacccctga cggaatggct ggaaaccctc tatccctatc
    11581 tctgccatgt ccatctccat gacaacaacg gcgatgtcga tgcccatctg cccgtagggg
    11641 aaggaaaatt tcccttcgcc gacctgtttg aatttctcga agcaaagggc tgtcatccga
    11701 tcttcaccat cgaggcccac agcgagcaga acctcatgag atccctggaa aatttggagc
    11761 gtctgtcctt tctcgggggg gatgccactt gctgaagtat atcctcaagc gcctgctctt
    11821 catgattccc atgctgatcg gcatcaccat catctgtttc gccgtcatgc atctcgcccc
    11881 cggttcaccc acggacctcc agacccagat gaatccccgg gcctcggcgg aattgaagga
    11941 gcgtctccgg gccatgtacg atctggataa gcctctatat gtccagtacg gattgtgggt
    12001 ccagcgtctc ctaattctgg atctgggaaa atccttcgcc ccggaccgcc gccccgtagc
    12061 ggaaaagatc ggtgagcgtc taccggtgac cgtgaccctc aacgtcctgt ctctgctttt
    12121 gatccttgcc gccgccattc ccctaggggt gctctcggcg gtccatcagg attcgtggtt
    12181 cgatatggcg gtgagtgtct tcgtctttgt cggttttgcc gtgcccacct tctggctggc
    12241 cctgctcctc atgatatttt tcggggtgga tctgggttgg ctgcccatct cggggctccg
    12301 gtccctgaac tacgaatatc tcccgccggg cgccgccttt tgggacctcg tgaagcacct
    12361 gatcctcccc gtgggtatct ccgccttcgg gggcctggct gggttgtccc gctacatgcg
    12421 ggccaacatg ttggaggtga tccgtcagga ttatatcatg acggctcggg ccaagggact
    12481 gagcgaattt caggtgatat acaagcatgc cctccgaaac gccctgctgc cggtgataac
    12541 catcctcggc ctttccatcc cgggcctcat cggcgggagc gtcatcttcg agacgatctt
    12601 tgccatcccc ggcatgggac agttgttcta tatgtcggta atggcgaggg attatccgac
    12661 cataatgggc atcctcttca tcggcgccgt cctcaccctg ctgggcaatc tcctggccga
    12721 tgtcgcctat gccgtggcgg atccccggat cagggtgggt taggagtagg gcatgggtag
    12781 cgaattccag taccggttgg cgggaaacag gatggccctg gccggcggga ttatcgtcat
    12841 cctcctgttt gtcgtctctt tggccgcgcc gttgctcgcc ccctatgatc ctctggccat
    12901 agatctgaca cggatcttgg ccccgccctc atggcagcac tggtgcggta cggatcaact
    12961 gggccgggac gtcctgagcc ggatgatttg gggggcgggc atctcgttga aggtgggttt
    13021 cgtggccacc ggcatcgccg tgttgatcgg cgccctcctt ggtgccctgg cgggatacta
    13081 cggcggctgg tgggatatgg tcatcatgcg cctggtggat gttatgctct gcttccccac
    13141 ctttttcctc atcctcgcag tcattgcctt tctggagccc tcgatatgga acatcatgat
    13201 catcatcggg gcgacgggtt ggatgggcat tgcccgcctg gtccgggccg atttcatgtc
    13261 cctcaaggag cgggatttcg tcacggcggc ccgggtcctc ggggccagcg acacccggat
    13321 catcttccgt cacatcctcc ctaatgccat gacttccatt ctagtggcgg caacgctggg
    13381 ggtggccggg gcgatcctca cggagtcggc cctgagtttt ctcggcatcg gcgtccagcc
    13441 gccgacgcca agctggggca atatccttac cgcggggaag gacaacatcg atatcgcctg
    13501 gtggctttcc ctctatcccg gcctggcgat cctgatcacg gtcctgggat acaacctgct
    13561 cggggagggg atccgtgacg cccttgatcc ccggctccgc ggcggtcggt agagaataaa
    13621 aaaggctcct gataaacagg aacctctttt atttaaagct aaaaaggtgc gcttattact
    13681 tcttctcagc ggcggcggcc ggtgctttct cctcggcctt ctcagccttc ttggccttct
    13741 tggccttctt ggtcttcttg gccttcttct ccttcgcggg ggccttctct tccgccttta
    13801 cgggctcagc ggcgggcttt gcagccgggg ccgcctgtgc gaacgccaga ccggacatag
    13861 aaagggcgaa aaggatcgaa acgacgatcg agagcagttt cttcatttag ggtgtcacct
    13921 cctttcattt tttatcctac ctaaacaaga agcgtgccat aagcggcgat tgatgaatat
    13981 cctgctatat caagatattg tgattgtggc gggggatttt ttcggcccac cggcggcatg
    14041 atgatgggga aatattcccc cttttgtgag gaaatattcc cgccgcgccg cccggcgcct
    14101 ggtggggcgt catggcagag gcggtggttt atgcttgagc agattgagca gcttgtcccg
    14161 gtaaagatag gcggcgacga cggcgaggag actgacggcg gccaggatcc acaggggtcc
    14221 gtgctgttcg tttctcaaca tgtcgccgct gatggacagc atggcggttc ccaggagccg
    14281 ccccagggtg gagacgacga gaaacatccg caccggcatg tggctgagac cgattatgta
    14341 gcacagggcg tccttgggaa agccggggat gagaaacagg atgaagacca cccaactccc
    14401 cttgtgcttc atgaagtagt cgtatttctg aaggatcgtg gggcggacgg ccttttccac
    14461 aaagggcagg cccagccagc gggccaggga aaaggcgagc caggagccga tgatgagggc
    14521 gatggtggaa tagatcgtgc cgaggacggc gccgtaaatg tagccgccga tgaagccggt
    14581 cgcttctccg ggaaccggtg cgaacaggac ctggaggatc tggaggccga tgaacaccag
    14641 caccgacagg ggaccgaatg aattgataaa atcgatggcc ttgtttcggt tcgtgaagaa
    14701 ctggataaga tcataatgaa tgaaaagcca ggtcccgccg ccgaaaagca acccgaataa
    14761 aaggatcttc agaagcgtgt tcttgttcat cccgcggctt tggcctttct tccccatggc
    14821 ggcccgagac gaccggccac ggtacggaga tcgttttggt ttacgtttcc ttattgaacc
    14881 tgtcgcctgc cgtcaaggac aaagtcccgg ccggagatcg ggataatccg taaagacgcc
    14941 gtcggcgccc cgggcgagca taatatccat atcctttcgg gtgttggcgg tgaaggcatg
    15001 gaatttcaag ccccggcgat gggcgtcggc gatgaatttc tccgtggcgg ctttcagggt
    15061 gccatggacg gcataggccc ccatctcttc ggcgaaccgg gcatagcccc gggggacggc
    15121 ggtcacgagg ggggcgatgc gcaggtctgg tcgcaatcgc cgcaaggcga tcaattcctc
    15181 gtagttgaag gatgatacaa ggagttgttc cgggtccgtc cgggctccct gcggggaggc
    15241 ggcgaggagg gcggccaggg ggacggcggt ggccgggccc ttgagctcga tgttgaggcc
    15301 gtcacccgga ccgagcatgg ccagcacctc ttcaaggagt gggatcttct cccctttccc
    15361 ggcatcgagg gagcggagat aggccagggt ctggtccatg acgtaccccc ggccattggt
    15421 tgtcctctcc agggttgcat cgtgaatgac cacaagtgcg ccggctacgg catagacgtc
    15481 caactccagc caccgggccc ccatggccat ggcccgccgg aaggaacgga gggtgttttc
    15541 cggctcgtag ccgctggccc cgcgatgggc gaagatggtg aactcctcgg ggagaccttg
    15601 ttgccggtct gtgccgacgc ggtacattaa ctgtcaccaa gaattttaca atcagggcga
    15661 gagcttgaca aaactgccgg cgcgcaacgc aaaaaccttt tgctcttttt cgtcttcatt
    15721 cctgttacta tcaataatcc caaacagttt ccccatcaga gcaatgacag caggatccgg
    15781 acaagatcct ccggggccgt ctgcatgggg tggtgtcccg tggcgagctc aaagaacggc
    15841 caaccgtccg cccgtacccg ggcggcgacg ccgatgattc cctggtgctg gggaccggcg
    15901 ccgcttttat ccgtgcagta gatatatgcc tgaggaatag cccgggcctg gggattgttc
    15961 cgggtcagcg cctggaacat cgtgcgggcg gggtgatcga caaaacgggg gtcgccccgg
    16021 ggtttcgggg gcggatagcg ccagccctct ccccgggcct cgatgtaggc ggcgagaagc
    16081 tcggctgtat catggggata caggtctttt acggcctggc cgtcctccgg gatcagggca
    16141 tcaagataga tcagcctccg gatacgctcg ggcatccttt ccgccacgcc ggcaataacg
    16201 gcgccgccgt aactgtgacc gaccagaatg atatcgtgga ggtcttcgaa tttgaacacg
    16261 ttgacgatat cggttatgtg ggtgtccaga tcgatgtccg gccgtaacag gtgggacctc
    16321 tcccccagcc ccgtcagggt gggggtatat actgtatgtc cctgccggcg cagcatctcc
    16381 ggcaccggcg accagatcca gcccccggcg gttgccccgt ggacaagaac ataagcagcc
    16441 ataagcgatc aaccctctct ctctttaccg gccgtcaatg agacatcttc aggaccgagc
    16501 ggatcaggtt ggccgtggtg tattggccgt cggtcatctg tgcgaacagg tgctcgccgc
    16561 cgaacagctc caccacgatt tccgtttcgc tgtgaccctg ctgctgaagg gtccgggcct
    16621 tacgggccag atcccggagg taacggatgt tctcttccag ggcggcacgg ccgttctcca
    16681 cgatccgccc cacggaggtg aagagcacca acttatccgg tgccgccgcc attatcctct
    16741 ccagggagcg gatcgtttcg cccatgtcct cttccggccg gatgaacttc ggcttctgac
    16801 gggcaaagat atcaccggaa aaacaccagc cccggttctt ttccagaagc gcaacgtggc
    16861 cgtcggcatg tccgggcgtt gccatgacct cgaaggtgta aaggggagtg gtaatgactg
    16921 ccggtacggg cagtaccggc gaaggctccg ggcgacccca gacaaattcc tgatacgggt
    16981 acaggaacgg tttgccggcg atgatggaaa tggaggccgg cgcggcatac accgggacgg
    17041 aaaaatattt ttcaatcctg gcgtttccgc cgacatgatc ttcatgaaag tgggtgttga
    17101 ccaccatggc cagtttttta cctgcgagaa attccacaag ttccgcggcg gtgtgagcgc
    17161 atcccgtatc gacgaggagt ccgtccacga ggtaggcggc gacccagtac agggccttgc
    17221 cttcccattc gcgactcatt cttatctgaa ttacgtcttc atattccttt atttccagca
    17281 tgttgtatcc ttttggggat aggttctcca cctccgcacc tgaccgcggc gcctcactgg
    17341 ccgggaagga ccatgcgacg gggcgggtaa ggacgaccat tcgtttcccg ccgtcgcgga
    17401 actccgaaaa attagccgat tacccgccgg gtgtcaaggg tcgatagggc ggcaagggcg
    17461 gaaaagtatt gacatggagg cgagttttca ttaaaaccaa cttcccgttg ggccttgctt
    17521 gagagaagct ttggcgatag aacaagacac ccgccgcggg gatgaaggaa aggtcgaagc
    17581 ggagcggggc gacactccag atgggcgggc gccggccgcc ccggccgtga gccgggacgg
    17641 gaccaataat ccaaggagaa accaatgctt tttaaaaccg ccattaccga gatgttcggc
    17701 atcgaatatc ccgtcatctg cggggccatg atgtggctct gcaagccccg gttctgcgcc
    17761 gccgtctcga acgccggcgg aatgggcaac ctgacggcgg gcaattacga aaccgaggaa
    17821 gaattccgcg ccgccatcgc cgagacgagg cgccttaccg acaagccctt tatggtcaac
    17881 attaccctcc tgccgtccgt gcggatcacc ccggagcatc acaagatgta catccgggtc
    17941 tgcaccgagg aacgggtcag cggcatcgag ttttccggga cgccggtgga caaggtggcc
    18001 ggcatggccg ccatcgagga tctcaagcgg gcgggggtga agctctttca caaggtcggt
    18061 tcggtccgcc acgccctcca tgccgaaaag gtgggctatg acgggatcta cgccgcggga
    18121 atcgaagagg gcggccatcc cctggatgac gatgtgacca ccatggtcct cacgccccgg
    18181 attgtcgatt cggtccgaat cccggtggta accgtgggcg gtgtcgccga cggccgggcc
    18241 ctggcagccg ccctggtgct gggagcccag ggggtcatga tggccagccg gttcgtggcc
    18301 acccgggaat gcgaggttca cgagaatatc aaacaggaac tggtccgtcg gcaggaaaac
    18361 gagacggtca tttatggaaa gtctctgggc ctccagggcc ggggactgaa aaccaaggtc
    18421 ctggaagagg tgtgcgccat cgagaagcgg ggtgggggtt ttgccgaact gattcccctc
    18481 ctttccggcc agagggtcaa ggaggcctgg gaaaccggag atgtggacta tgcgccgctc
    18541 atggtcggcc agtccatcgg cttcatcaat gatatcccca cctgccggga acttctcgaa
    18601 cgcatggggc gcgaggcggc ggcatgcctg gaccgggcga agaaatcgct ctaggagagt
    18661 tgagataaaa aaatggcgaa acagatttac gaaggcagca tggacaagcg gatcctgcag
    18721 atgaagatca gggctgggga gttgaaggag gaggacctgc gggagaattt gaagaacctt
    18781 cccgatgtag ccgccaatgc ggaaccattg accacggtga tggagaggca ggacgacggc
    18841 ggtgcttcct gatcaagagc atgttcatcg actcccacgc ccatctggaa atgagagagt
    18901 tcgaccggga tcgggaggag gttatcctgc gggctgcggc ggccggggtc gattacatcg
    18961 ttacggtggg tacaacgctc cgggagtgcc gcaaggcggt ggagatcgcc cgaagacatc
    19021 ccgccgtcta tgcggcggtc ggcatccatc cccatgacgc caaagagatc gaccataata
    19081 cctacgacgt attgaaaaca ctggccggtg atgaagcggt ggtggcatat ggggaaatcg
    19141 gcctggattt tttccgcaat ctgaccccgc cggagatcca gcggcggcgc ttcgcggagc
    19201 aactggaggt ggcggcggac ctgggcctgc ccgtaatcat ccacgaccgg gaagcccacg
    19261 gggagaccgt cgcgaccctg aaggcatggc gagggcggac ggggggcgtg atccactgtt
    19321 tctccggaga tacagccatg gccatgacct gtgtggagat gggtttccat atctccattg
    19381 ccggccccgt gacctatgcc aagtcggagc ggttgtggga ggtggtacgg caggtgccgc
    19441 tggagcgaat gttgatcgaa acggacgccc cctaccttac cccccatccg cggcgcggcc
    19501 ggcgcaacga accggcctgt gtagtcttga cggcccaaaa ggtggcggaa ctcaaaggtg
    19561 tttcccgcga taaggtgggc cgggagacgt cgcgcaacgc catgctgctc ttcgatattc
    19621 ccaggaaggc aaaggaatca tgaccggcga actcctctat tttgcctacg gttccaattt
    19681 gaatcgggac gatctggcca aatggtgcca ggagcacagg ttcgactatc ccctggggga
    19741 gaaggtggcc aacgcctatc ttcccgatgc ccgtctgatc gccaattact attcccactt
    19801 tcgccggggc ggcatcctga acatcaaata ccagctcggg caggtgacgc cgggggcgct
    19861 tttccgggtc cttcccggct gctggaacgt tctggacatc aagggcgaca cggagaatat
    19921 gtacaagcac cttaatatcg tcgcccttac cgacgacggc cgggagcatg tcgccgtcgc
    19981 ctatcagatc gatgcctcga aaacctcccc ggtcttcgtc cccctccatc ccgattatcg
    20041 ccgcatcgtc cgggaaggga tggccgccca tgggatcgat gcggacgtct tcaccgtagt
    20101 ggcggaagga cgagacccgg actatccgat caaacacatc ttcgtttacg gcaccctgat
    20161 gagcgaagga ccccggcatt atctcctggc ggcatgggcc gatctcgaat cccgcttgcc
    20221 cgcccgggtc cacgggctcc tttacaacag ttggaagggg tttccctgta tgatccccga
    20281 tgtaacggga cggtcccacg tgatcggcga gatctacacc ctgacggatc tgaaaaaggc
    20341 cttcgaaata ctcgacatcg tggaaacggc caaacgctat gccgccaccg ggaccttctt
    20401 ccgccgggcg atcatcgagg tgacccggga ggacggctcg aaatgccccg cctggacata
    20461 tttcgtggac atgagtgtcg aagggatgcc ccgcatcccc tcgggaagtt ggcggaccgt
    20521 catcgcctaa ataatccttg catttcccgg catgaatttg taaagttccc gtagaccccg
    20581 tgaaacggcg gcggatgcag cgcccgcctc cttccggggt cgtcagatac atattcgaca
    20641 ggggcgccgg tacggctgag aagctaccct ttgaacctga cctgggtaat gccagcgtag
    20701 ggagtggcag cagaacgaac gaatttttgt ggggccgtct cccatcccgg ggcggcccca
    20761 ttgttttgcc gccggcgtcg gcaggcacgg ggagcgtccg atgacccgcg acggggtgaa
    20821 aagagatacg gccgcccttg cccgggcgac cgggaacgaa cgtcggaaag gaagaggcgc
    20881 gatgcccggt gacgggaaaa aaaggattgc cgtcctggcg ggttccccga tcggagatct
    20941 ggaatggctt cgacgggaat tggctgccgc cgctccggaa gccgttctgt gcgccgacgg
    21001 cggcgcccgt tatgcccggc tccttggggt ggttcccgat ctgatcgtcg gtgatctgga
    21061 ttcccttacg gaagagctgc agcgcgcctt tcgggatgag ggggtcgcca tggacgtcta
    21121 tcccgccggg aaggatgaga cggacaccca gttggccctg gagcgggccc tggcgatggg
    21181 ggccggggag attctggttt tcggcgccct gggaaaccgc ctggaccaca ccctggccaa
    21241 tatcggcctg cttctcctgg ggaggaatag gggagcggcg gttatcctcc gggatgagtc
    21301 ctgcgatgtc ttcgcagtga ccggagagtc cgtgatccgg ggtgaggcgg ggcagaccgt
    21361 ttccgtcttc cccttggacg gtgatgccgc ggggatcgac cttgccggtt tcgaataccc
    21421 cctggacggc gccgccatga ccctatcccg cccctacggg atcagcaacc gcctggccgc
    21481 gccgacgggg cgcatcaggg tcggggaagg ctgtcttttg gttatccatt atcggcgcgc
    21541 cgtggcttag tccggtgcgg ggccggacag aacaaaggag atcgttttga aggggattaa
    21601 gaggatattg accatcgccg gttccgattc cggcggcggc gccggcatcc aggcggatct
    21661 gaagaccatc accgttttgg ggggcttcgg gatgagcgtc attacggccc tgacggccca
    21721 gaacaccctg ggggtccatg ccgttcacga ggtgccggtg gaattcatcg ccgcccagtt
    21781 cgacgccgtg gtctcggata tcggtgtgga cgccgccaaa accgggatgc tttcctcggc
    21841 ggccgtcgtg gaggtggtgg tggaaaaaat cggacaatac gggatcaata agctcgtcgt
    21901 cgatcccgtc atgacggcca agggcggggc gacgctcatg gaacccggcg cccggaaggc
    21961 ggtgatggcg aaactgctgc ccctggccct ggtggtgacg ccgaatatcc ccgaggcgga
    22021 gatcctcacc gacgtcatca tcgcttccgt ctcggacatg aaggccgcgg cccgaatcat
    22081 ccacgagctg ggggcgcaaa acgtgatcgt caaaggcggc catctcatag gcgccgcggc
    22141 ggatgtcctg tatgacggcc atgattttta cgagtttccc tttgtacggg tcgcgacgaa
    22201 ggacacccac gggactggct gcaccttttc cgcggccctg gcgacggccc tggcccaggg
    22261 ccggtccgtc cgggaggcca tggccgccgt cgaggattat atggccgccg tcatcggaaa
    22321 ctccctgcgc ctcggggcgg gtcacggacc tgtgaaccat tgggtgtgtc cggcgccgtc
    22381 cggcgggacg gaaagatgaa cgagtaaaaa tgacgacata gtaaaaaacc ctcatgcccc
    22441 gtggagcacc acgaagcatg aaaatggcat tttcataccc aaccctcccc ttgaagggga
    22501 gggccatttg cgactttttt acgatgtcgt caaaaataac acgcggccgg aaggaaaata
    22561 gaaaagtctg ccccggccgc cgggagcggg agacatgacc cagatagaac aggcccgaca
    22621 gggcatgatt acggaagaga cgaccattag cgcggcgcgg gagggctgcc ctcccgagta
    22681 catccgcgcc ggcgtggcgg cggggaccat cgtcgtcatc aggaacaagg tccacggcgc
    22741 cattgcgccc ctggccatcg gcaagggcct gcggaccaag atcaacgcca atatcggtac
    22801 ctccaaggac cgggccgatc tggtggacga actggaaaag gtgcggatct gcgtcgaagc
    22861 cggcgcggac accatcatgg acctctccac ggggggcgat atccaggcga tccgccgggc
    22921 catcgtggcg gcttcccccc tggtgatcgg taccgtcccc atctatcagg cagcggcccg
    22981 ggtgctggcc cggggcaagg ccatcgtgga gatgaccgcc gaagacatgt ttgaggtcat
    23041 tgaggaaaac ggcgcggacg gggtggattt catcaccgtc cattgcgggg tgacccggcg
    23101 gagcgtggcg gcggtggagg cccagggccg cattctgggc attgtcagcc ggggggggtc
    23161 cattacggcc cactggatgg aatgcaacgg cgaggaaaac cccctctatg cccagtacga
    23221 ccgactcctg gatattgccc ggaaatatga aatcgttctg agcctcggcg acgggctgcg
    23281 gccggggtgc ctggccgatg ccaccgaccg ggggcagatc caggagttga tcctcctggg
    23341 agagctggcc aaaagggccc gggaccgggg cgtccaggtg atgatcgaag ggcccgggca
    23401 cgttcccctc accgatatcg agagcaacat cctcctccag aaacgaatct gcgccggtgc
    23461 ccctttctat gtcctcgggc cgttgcccac ggacatcgcc ccggggtacg atcacctcac
    23521 gtccgccatc ggcggcgcca tcgccggggc ggccggggcc gattatctgt gctatgtcac
    23581 cccggcggag catctccgcc tcccgaccct ggaggatgtc cgggaaggga tcattgccgc
    23641 caggatcgcc gcccatatcg ccgacatcgc caagggattg ccgggggcgg cggaacggga
    23701 caggcacatg gcggtgtgtc ggaaggaatt cgactggcaa ggacaggtcg aagcctccct
    23761 ggacccggaa cggaccggcg ccttcctgga aaagagcgcc agcgcccggg atgaaggatg
    23821 taccatgtgc ggcgaattct gtgccatcaa gctgggccgg atggccaaag acagggcgac
    23881 cggggcgaaa tgctgaagga tgcctttgca cggcccgcga agatcgccgc cagcggtcgg
    23941 gtcatggaag cgaggaggag aagatgatgc aggtgctgtg gacattcgta atcgtttcgg
    24001 tggttttatt agcttttttc ttcctccgcc cgccctggag cctggagaat atcatcatcg
    24061 gcggcgtccc cctagtgctg ctggtgatct tcgcctgccg cttacggcag gcaaaaaagg
    24121 agcgggccgc cgctcccccg gaagacgacg acgaagaagg atgaaggccg ccggatggtt
    24181 ttgcccttga cggtgaacgg agcggcgtcg aaacgggacg gggcggtaag catcgcacgc
    24241 gccctgcgcg gggcgggtca cgaggccttt ttcgtcggcg gctgtgtccg tgacctcctt
    24301 cggggggaaa ccccggatga ttacgatatc gttacctccg cccgtccgga acaggtcatg
    24361 gccgtgttcc cccgcactta ccccgtcggg gccgctttcg gggtggtcct ggtccgggag
    24421 ggggaacatg cctacgaggt ggccgcctac cggaaagaag cggattacga ggacgggcgc
    24481 cacccttccc gggtgtcctt cgtcggcgcc cgggaggatg tctgtcgccg ggatttcacc
    24541 gtcaacggcc tcctcatgga tcccgagacc ggggagatcg tcgattacgt gggcggccgg
    24601 gcggatctgg aaaagggtct catcaggacc atcggcgccc cggaggagag gtttgccgag
    24661 gatcatctcc gtatgctccg ggcggtccga tttgccgcca atctcggctt ccgggtcgag
    24721 gacgccgcgc tggcggccat cattcaccag gcggacaaga tcctccgggt cagcgccgaa
    24781 cgggtccggg aggaattgaa ccggatcctg gccggaccca gaccccgtcg ggggatggaa
    24841 ctgctcgctt catcggggct cctgtcccgg ctcctgccgg agctcgtcgc cctccggggc
    24901 gtggagcagc cgtcccgctt ccatcccgag ggcgatgtgt gggagcatac cttacgcatg
    24961 ctggccctgc tgcctgcgga aaatcttccc ggagaggaag ccgaacggct ggcctgggcg
    25021 atcctctttc acgacgcggg caaaaccgcc acccggacgg aagatgaaac cggaattcat
    25081 ttctatggcc atgtgtccaa aggggtggaa atcgccgcgg cggttctgaa gcggctcaag
    25141 ttttcccggg aggacgggga ggtcgtcctg gccctcgtgg cgaaccacat gcggttcatg
    25201 cacgtccgcc agatgcgggt ccacaccttg aagaagtttc tgcgcctgcc cgacttccac
    25261 ctgcacctga tcctccatcg cctcgactgc ctggggagcc acgggctcct ggacaatgaa
    25321 gccttctgcc gggaacggct cggggagatg accggggagg atctgcatcc ctcccggctc
    25381 ctgacgggcg acgatttgct ggccctgggg ttttctcccg gtccggtgtt tcgggagatc
    25441 ctcaccatga tcgaagacgc ccagctccgg ggcgagatcg ccacgcccgc ggaagcccac
    25501 gccctcgtca ggaggaagtt ttttccaact gccggcgcag gcggtcgtgg atgaggcggg
    25561 aagcgatatt gctccccccc aggccgacga ggatcgacac gtcggtcttg tccttgtcct
    25621 tgaactccac ctcgaaggtc accttttccc cctggatgac cgccagcacc cgggccttgc
    25681 cgatcttgac ggtcttttcg ttgactaccg cctgcatctc cttgagcgtg tcgtcgaggg
    25741 cacggcaaac cgtttcgaac ggccagggat aggaggaaac gacgtaaccg ctggactgga
    25801 ctacctgtcc gtcctgtacg gccacggatt ttcccccgag gaccagcccg ccctcgcagc
    25861 cggccagcac ggtcaggata aggatcgata atacagcaat aaagttattc ttcatcggct
    25921 ttcctcctgc aagttttata taaccttcga caagatcgat ttcccatgcc ggtcttgatc
    25981 cggcatccag tgtctttagg ggtggaggta agatcagcgg aaataggcgg catgctgcag
    26041 aagctgggaa aacgcctgaa agaccgccca gaccaggagg atcgcggtgc cgtagatgca
    26101 ggccttgccc aggacgcgat gcccagccgt ttcccgctgc cgctcctcat agcccaggat
    26161 gaaggcggcg gcgacgccgg ccgccaggcc tcccccatgg gcccagttgt tgatccccgg
    26221 tacgagaagc ccgaagataa ccaggcccac cacccagccc atggcctggc ggtacatggt
    26281 cattccgaag taaccgcccc ggctacgggc gaagtagaga atggccccga tgaggccgca
    26341 gatgctcgcc gaggcgccga tagtgaaggg gatgccggcg aaaaaggaca ggagaaaacc
    26401 ggcgattccc gtcagggtat agatgacggc gaagcggtgc acgccgaatt ccgacaccac
    26461 gaagggcccc agttgattaa gggccatcat gttgaagaag atatggagca gcccgccgtg
    26521 taggaaagag gcggtgatca gggtccacca gcggtgaaag tggccgatgg gcagggtccc
    26581 cgtggccccg aggagaaaga ggctcccttc cgaaggagaa aggagcatca gggggttcat
    26641 cgtcatcccc atcccccggg gattcataag cagggagagg atatagaagg cgacattggt
    26701 gtaaataatg atcctgacga ggtccccgtt gcctcccgcc cagcgggaca aaaaaaagcg
    26761 catccggcgc gaaccagggt tggcgaggcc gcaataggga caggacttct catcgagact
    26821 gatcagcttg cggcagttgg ggcagagtcc ggatgttctt tccgccgtca tctcagcctc
    26881 cggtgaagaa gtatttcatg atgaccggga tcgccaccag gacccccgcc atcgtggtca
    26941 ggttcacgaa cgccaccagg atcagcagcc tggtgatctt gttgcggaaa aacccccgca
    27001 ccgagctgat gtcgtccttc agttccagga agtcccgaac ctggggtttc cgcagtgtcg
    27061 cctcggtcag cccggcgacc cagccggtgg cgaccagggg gtggatggtg gcgatgggcg
    27121 cggaaaaggc ggcggcaagg atcgtcaggg ggtgagagag catgatgagg gcccccaggc
    27181 cggcgcaggc cgcggtgatg gcggaccagg aggcgaccat gtgcatgctg gtccggccgc
    27241 cggaatagaa aaacccggcg atgaacagga ccacgatcgc cgcggaaaaa acccatccgg
    27301 cgtaccggcc ccagattccc ggcggcggga cggcgctgat ggcgtccata tcgacgctcc
    27361 ggcccaggtt ccttttgatc cctgggatgt gtcccgcgcc gacgatggcg acgattttcc
    27421 gaccgtccgc atggctgatg gtatgggaga ggtatgcatc ccgttcgtcg atcagggtgg
    27481 ccttcagggt cggcagcttt tccccgaagg tcttaagggc cacttcgagg acgtcctgtt
    27541 ccttgagctt ttccacttct tccgcggtga tgtcttcggt atagaacaac gagacgatca
    27601 tctcgggcag gacccggatt ttggtccaga agcccatctt ccgccaggcc cgcaggaggg
    27661 tgacacggat ctcccggtcg gcgaggacga cggcgatccc cagcgcttcc gcccggttga
    27721 cggcgcggat catctcctcg ccgggggtga tgccgagctt ctcggcgatc tttttctgga
    27781 aggaggccaa tagcaactgg aagaggagga ccgaggtccg tttctcacgg atgaccttga
    27841 cgatatccat ttcctgccat ttttcgcgct gtttgatggt ctcgtaacgg gaattgcaca
    27901 gctccacgca tactgcgtcg gggtgctcgg cggcgatgac ggcctcggcc agctcggcgc
    27961 tttcccggga gacatgggcc gtgcccacga gcaccagttc tttaccttcc cagcccaggc
    28021 ggtggatgtt gggatgatcc aaaaatttat cgctccataa acagggtttc gcaagcgggt
    28081 cattagcagt aaaaatatgg attgtaaagg gaaagaaaaa aacgtgaaat tgaaaatgtt
    28141 tgttgatttt ccccggattt aaggtaaagt gcatcattga aaaatgtaag tcgatcgact
    28201 taatcaggaa aagtacctag gtagtaattg gatatagttt aatataatca tatggttact
    28261 gcccaccaca agcggaccgg caggaaattc cgttacctct cccttatgtc ggcatccaac
    28321 cggcggcccc gcttccgttc cgcctttgac tatgccccgc ggagatttct gatcctgggc
    28381 gatccggaat cgcttcgata tattttactc cataccaacc gtgattcccc ggctagagct
    28441 ttgtgttaac accccggtgt cattaatttg cctattacat caatgataaa agctcatctt
    28501 cggagggttc gatatgaata agttaatgct ctggagcctg cggaacgcaa agctcgttat
    28561 tatcatcaac gtggcgatca cgatcgtttt cggctggttc gccctgcaga tcaaggtgga
    28621 ttcctccacg gaagggatga tgatcactgg cgacccggcg aaggattacc accaggacac
    28681 ggtgcggaag ttcggcagcg acaacatcac cgtcgtcttc gtccgggaca agaacctctt
    28741 ctctccggaa aaactgaaag atgtggaaaa gctttactat gagctcaagg acatcaaatt
    28801 tgagccgaag gatcccgagg aatacgtccg ggccaaggac cccgaggaat acctgaagat
    28861 caggtatccc gcggtatatg aaaaaatatc cgcccatgcg gaagaatacg ccaagctcac
    28921 caagagtcgc gaggaataca acaagctcgt ctatgaaccg gagacctatc tgaagcacaa
    28981 gttccccgac acctacaagg agctcctcaa aggcaagacc cagccgtcca aagaggaatt
    29041 gaagcagcag ttcgtcgagg ccgtggagcg gatcggcaag cccggcgtgg ggcgctgcga
    29101 gagtctcttc tccgtgacga atttcaaggg cgtaagcggg gcgctggaga ccaatcccct
    29161 catggaccgc gcccccgaaa cccaggccga cgccgatcag atcaaggctg acgccctgaa
    29221 gagccccctg ttcatgaaca gcctgatcga caaggagggg cagatgacgg ccatcaacct
    29281 ctatgtcgat gtcgatccca cggaaccgga gtttcacacc tatttctccc ggaaggttga
    29341 cgagatctcc ggcaagtacg agaatctctt cgaccgggtc ttccagttcg gcaactccta
    29401 cggccgccgc aacatcggcg agaatatcat caaggatcaa agccggatcg ttcccctggc
    29461 ctgcgcggtc ctcattggga cgatcattat cgccctccgc aatgtcggcg ccggtctcat
    29521 gcccatgatc accgccggca cgagcgtcat ctggaccttc ggcttcatgg tcgtggcggg
    29581 catccccctg aatgtcatca cggtgatcat tccctcgatc ctcctggtca tcggatccgc
    29641 tgaagacctc cacatgatgc acgagtacct ggaggggatc gaggagacga acggcaaccg
    29701 ggacggttcc atcaagctca tggcaagcaa gctgggcctg gcggtcctga tgacctcgat
    29761 cacgacgatt gtcggtttcg cctccatcat gtacaacgac atctccatgc tgatccagtt
    29821 cggccaggtg gccaccttcg ccctcaccgc caactcgctg agcaccttca ccttcggacc
    29881 cgccctcctc catctcttcg gccccgtgaa ggtccggaag cacgaggaaa aaaagcactt
    29941 catcgatgcc tttttccacg gcatcgccgt ggggaccctg aagctcgtca cgcctaagaa
    30001 gaataagatc atcatttccg tggtcatgat tctcctgacc ctgggcatgt tcggcagcac
    30061 cttcttcgtc aaggtgaaca acgacttcat gggctatttc aaagcggatt ctgagatccg
    30121 gaagcggagc gacacccttc ataaagaact ggcgggggcc cagacctttt tcatccgcat
    30181 caacagcggc atgcccgaaa ccttcaagca gtccgaatac ctctcccagg tggcggccct
    30241 ccagtcttat atggcccaga agggctggtt cgacaagacg gagtccctgg ccgaccgggt
    30301 ggctttgatc caccgggaga tgaacaacgg cgacatgaag ttctatgcga ttccggcgga
    30361 ttccaatctg atctcccagt atctcctctt cttccagcgc gacgagatct cccgctttac
    30421 cacgccggac ttcagcgagg tgagcatcat ggtccgtcac aatgtaacgt caagctatga
    30481 aatccagcag atcctcgagg aattgcggca gtatatgagc aagaacatca acccgaattt
    30541 caagtatgag ttcaccgggg agtacatcct catcaacaag gcggcggaaa gcctggtgct
    30601 gaattccgtc acctccctgg ccttgaccct cttcatcgtc ttcatctgta tctatttcct
    30661 cttttggtcc atgaaggcag ggtttatctc cctggtcccg aatgtcttcc ccatcgtcgg
    30721 catcttcgga atcatgggcc tcttcgatgt ccccctgaac gtcggtaccg ccatggtggc
    30781 cgacatcgcc atcggtatcg ccgtcgatga caccatccac ttcatgagcc gctacaacca
    30841 ggccatgcgg gagcttcaga accgggaggc cgccgtggcg gtggcggtcc atgacgaggt
    30901 tcggcccgtg gtctgcagtt ccgtcgccct cgccggtggc tttgccatct gcgccgtttc
    30961 ccagttcatt cccgtcatac agttcggcat cctctcctcg gtggtcatga tcatcgccat
    31021 cttcagcgag ctcttgatca cccctattct cctccaatcg acacagctca tcaccctgtg
    31081 ggatatgatc ggcttgaagc tccaggacgc ggtggtgaag acctcgccct tcttcgaagg
    31141 cctccgtccc tggtcgcggc gcaaggtctg cctcctgggc cggatggtgg agaagaacgt
    31201 cggtgaaaaa gctgtcgtcc agggcgagtt tggtaagagc atgtacctcc tcctggaggg
    31261 caaggccgag gcggtaggcg tggatgccaa aacgggcaag gagatagttt ttgcctccct
    31321 ccatccgggg gatatcttcg gcgagatcgc cctggtcaac cccggtcccc gctccgccga
    31381 cgtccgggcg gtggagcccg tcaagtacct ggagatcgac tgggaaggcc tcaagcgcat
    31441 ccagaagatc tacccccgga tcggcggcca gttgttcctc aacctctccc ggatcctggg
    31501 cgaaaaactc gtccatacca acgagatgct cttcgggaag gcgtaagggg cctgtcggac
    31561 caaggcgcat cgatttgccg catctcccgg ggggacggat tcccagttcg gtcccccggg
    31621 gtttttttgc cggcggcgta acggcggggg acggcctccg gtccggtaac gcaccggatc
    31681 ttgccatcgg cgggtcggct aaccggcaaa cccgccgatg gcaagggacg acccggcggt
    31741 tcatccgcgg cgtcccgact ggtcccgtcc ttgtgtaaag atgccatagc tgatatgatc
    31801 gctcccaaat cacttgggaa tacagccgcg cctttatgat cgaatccttt cttctcctca
    31861 gttccgggat caccctattc ctcttcggca tgatgaagct tggggcgggg atccagcaac
    31921 tcttcaccgc ccgaatccgg gaccacatca aatacgccgt ccggcgcccc tttttcggtc
    31981 tcctgaccgg tgtagcggcg acggtcctgt ttcagagcag ttccgccacc accgtcatcg
    32041 ccgtggggat ggtcagcgcc ggattgatca ccttttatca ctccctggcc attgtcctgg
    32101 gggccgatct cgggacgacg gtcatcgtcc agttggtggt ctggaagatc accgacgcct
    32161 ccccgctgtt tgtcatcgtc ggcggcatca tctggtttac cgcccaggag aggcgcaaaa
    32221 gtctgggtga ggcgatcttc tacttcggcc tcctgttttt cggcctgagc gtcgtgggga
    32281 cggcgacggc ccccctgcag cagcacccgg agatcgtcgg ctttttccgg gaggccaagc
    32341 atcccctgct gggattcacc ctctcggcgc tgttcacggg cctgatccac gcctcggcga
    32401 tccccatcgg catcctcgcc attctcgccc agcaggatct ggtcagcctg gacaacgccc
    32461 tgcccatggt catcggggcc aatgtgggca ccaccgtgac cgcccttttg gccggtatgg
    32521 tctccggagt cggcggcaag cgggccgcct tctcccactt cttcttcaag gcggtggggg
    32581 cggcgatctg catggccgcc ctgccgccgt ttctcgcgtt gctgcgaagc ctgtcgcccg
    32641 gcgtcgccca gcagatcgcc ttgggacatc tcctcttcaa cctgatcatc gtcgtccttt
    32701 tctttccctt gctccgtttg gtggcccgcc tcctggtccg tatcctcccc ggggaggaaa
    32761 agaccctccc cctgtggccg gagtttctca aggaagacgc cctggcggat cctgtctcgg
    32821 ccctggaaat cgtctcccgg gagctggagc gccagatcgt catcgtggag gagctgtacc
    32881 gccgggcgac accgctgcgg accgcctatc gggaggggca gcggcgggac gtcctgtatg
    32941 tggagatgat aatcaatcac ctgcgcgccg agatcgtttc ctatctgcgg aagatctcat
    33001 gccaggatct ttccccagca ctgtcggggc ggcttttcgc ctatacggcc atggccgacg
    33061 atattgagcg catggccaac cacatgatca atctcgtttc cctgtcccgg gaaaagcagc
    33121 ggcggcggat cgagttcacc cggtttgccg aagaggaact ggatgagatc gagaggctgg
    33181 tggagatgaa tatcgccgac gcggtgcggc tgatccgcca aggtgacggt gaccggagcg
    33241 ccgccgtcac ccggcgggaa aacgagatcg acgacgccgt aaaagcggcc cgggagcgcc
    33301 atctggttcg ctttcacaaa cggctctgcc aggccgaggc cgggccggta ttcgtcgaga
    33361 tgctcatcca tctggagcgt atctccgacc attgccagaa tatcgccgat catgtcgccg
    33421 agctggaagc agcggaagtg tcttgaccgg tcggggatga aaatgatacc ttcccggata
    33481 atcaatatgt gaagtcgatt cggtagggac cacggatgag caaggaaagg agatcgttat
    33541 gaaggatcgt ttgaacgccc tggaagtagc cctgaacaac gaactgcggg aacacgagtt
    33601 ctatctgaaa aacgccgagc ggaccaagaa ccccgtcggc aaggcgatgt tcctgcagat
    33661 cgccgcggag gaactggagc attacgagcg cctccagcag ctccatgaga aatgggacaa
    33721 agagggcaag tggcccgaga cggtgccgct gaaggtcaag gagacgaagg tccgagacat
    33781 cctcaagaag gtggtccagg aggcgggccg gggggccgcc ggcgatgccg atgaccgcaa
    33841 ggccctggag acggccatcg atttcgaggg ccagggggcg gcctattacg cccgcttaag
    33901 agacagcgtt aaggatcccc gggaaaaggc cttcttcgaa cttctggcca acatcgagca
    33961 cgagcattac gtatccctga aggatacgga ggaatatctg ttggatccgg cggcctggta
    34021 ccggaatcgg gagcgcggag gcctcgacgg cgcgtaagac cgtcttcacc gcggcaggtg
    34081 cgcggtgaga taatcgatgg tcatgggcag atccagtttg agctgctgga tgaactggat
    34141 gccgatctcg atccttcccg tccggcaggc gtggagatgt cctccatagc ggcggtccgc
    34201 ggtgagaaaa tggaggtgaa agccggggaa gttcagggag cggatgaact tgggcgtata
    34261 aaagcccgcc aggacgccgt ggatgttttc aaagctcagg gcgggccggt tgtgatcttc
    34321 ttcgttcagg ggctgatagt tctcctgccg ggagacggac cacacctgca cctcctggaa
    34381 aaaaccgtcc acgcggatgg cgtagagcat gttgtccgag gggaggagac gccccaggag
    34441 cgcctggaag gccgcgtagt ccaactcgac cgcgaactct tcgctgaccg tgggccggta
    34501 gaaggtcacg caggcaaaag gggtctgcac ctcatccgcc acgggatagg tcttgccgtc
    34561 ggcccggagc cggtagatga tcccgtccag catcaccatc tcgccgtcga ggttgttgaa
    34621 ggtgcccaga ccgaaatcgc cgtggcgctt caagtccccc agcagggtgt tttcctcgta
    34681 aaagcccttg agcatggcgg tcaccggcgc ggagagatag acggcgctca ccgcttcttc
    34741 acctgaccgc cgccgtgcct ccagataatg cgccagggcc gcccgggtta tttccgtggt
    34801 ggtgtggccc acggccgcgg ccgcccgctt caattccccg accaggtccg cgggcagggt
    34861 gatctccatc tcaatggcgg gaatctgctg atccggcata acgactcctc ctgtgttttc
    34921 tttgaggttt tccgaacccc gcggtccggt gtttgtaggg catatcctct acattgaaag
    34981 aataactttc agacgccttc cagggacgcg gccagctcgt gccaccggtc gtagagggcc
    35041 tcgatttcct tttccaatgt cctcaggtcc cggaccgtct gtttgatccg ttccggttcc
    35101 cggtggctaa aaggatggca gagaaactct tcgccggcgg ctttgcgctg ctcccggtcg
    35161 gtgatgtccg cttcgacggc ctggaggtcc ttccgcagcg cccggcgctg tctggccagg
    35221 ctgttccgtt cctccgcctc ccggcgccgt tcctcccggg tccggaccgc cctgccggca
    35281 cctccgggga cctcaccggg gaactcgccg gtgcagccga cgaacctgtt tccccggact
    35341 tctccggccg cctcctccgc gccggcagtg gcggagatgg cacgcttcgc gtccgtcacc
    35401 gaggcgttgg cctctcccga cgtccgccca gagagcgtga cccccttccg atctcttccc
    35461 tccgcccctg cctccggggt ctttcccatc gccgacacat ctcgcgacga ttgccttttc
    35521 tcgatgaaat ccgagtagtt gcccagatat tcgcggcacg cgccgtcccg gagctcgaag
    35581 acgcgattca ccaggcggtc gaggaaataa cggtcgtggg agactagaac cacggtcccc
    35641 ggataggcca gcaaggcact ctggaagatg tccttggtct tgagatccag gtggttggtc
    35701 ggctcgtcga ggatgaggag attggtcttc cgcaggagga tcttcaacag cgccagccgt
    35761 gatttctccc cgccggagag gacggcgaca ggcttgtgga tgtcgtctcc ggaaaagaga
    35821 aaggccccca ggagattccg ccgctcctga tcgtggctgt gggaggggac gtccagggcc
    35881 tcctcccaga ccgtccggtc atagcggagg ttctcggcgc tttcctggga gaagaaggcc
    35941 atctgcacgt tgagccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    36001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntagc
    36061 ggaggttctc ggcgctttcc tgggagaaga aggccatctg cacgttgagc ccgtagcgga
    36121 tctccccggc ggaaggggct tccaccccgc ccaggaggcg cgacagggtc gatttcccgg
    36181 cgccgttcac gcccaccagg gccacccgct cgccccggtg cagggtgaac ccgactccgc
    36241 tgaagacggg gatgccgccg tattcctttc ccaggtcgcg gacggtgagc acctctttgc
    36301 cgcttttcgt ccccgccgga aatttgatcc ggacggcctg ctccgattct cccgcctcca
    36361 gggggctgaa tttctccagg agccgcagcc ggctctggac ctgtttggcc ttggtggcct
    36421 tgtaacgaaa tctctcgatg aacgcctcgg tccttttgat ctcactgtgc tgaaggtggc
    36481 gttccttctc cagggccgcc cggcgccgct ccttctcccg gagataccag gaatagtttc
    36541 ctttgtagat gatcaggcgg ccccggccga tttcggcgat cgtggtcgcc atcttgtcca
    36601 ggaagaagcg gtcgtgggcg acggtgatca gggtccccgg ataatccttg agatagcttt
    36661 ccagccactc catgctctcc gtatcgagat gattggtcgg ttcgtcgaga agcatgatgt
    36721 ccggccggga aaggaggatc accgccagga ggatccgcat cttccacccc ccggagaact
    36781 ccccgcagtt tcggtgatga tccgcttccc gaaacccgaa ccccttgagg atctggccgg
    36841 cccgggcctc gaagctgtag ccgtcccggg cgttgaaaag ggccagggcc gtctcgtaat
    36901 ccttcaacaa ccgccggtag gaagcggtcg tctcgtcttc ttcctggctt tccgtttccc
    36961 ccgtcgctcc ggcggcggcg ccggggaggc agcataccgc cgcggctatc tgcccttcgc
    37021 aaacccggag ggttttttcc agggcggcga tgccgcaccg gtcccggaga taatccatca
    37081 atgggacgtc gggcagctcc tcgagatcct gggggagata ggccagggac ctgttccggc
    37141 ggtcggtgat ctcgatggcc ccgccgtcca gctccacccg gcccaggatg gcccggagca
    37201 gggtggtttt tccggcgccg ttgtcgccca ccaggccgat ccggctccgt tccgtgatat
    37261 gccaggaaag gtc
//
LOCUS       DAOSMF010000010        37055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_1510_length_37055_cov_22.775405, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000010 DAOSMF010000000
VERSION     DAOSMF010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 37055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37055
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1510_length_37055_cov_22.775405"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(240..479)
                     /locus_tag="PLT63_02555"
     CDS             complement(240..479)
                     /locus_tag="PLT63_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97631.1"
                     /translation="MEYTDRTGKDEFPEQARNFLDRGLYQLARDLAETRLAEMPGDVE
                     GRVILCEALWGLGYDDEAQEIIREIEEIIRGYSEI"
     gene            533..787
                     /locus_tag="PLT63_02560"
     CDS             533..787
                     /locus_tag="PLT63_02560"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOD97632.1"
                     /translation="MPRSGKVRRHHVGDSAIQEMIKKAIKMAAIPKHGSVHTLRHSFA
                     THLLMNGVNIREVQELLGHKNVETTMIYTHVMRSILCKIA"
     gene            complement(821..1852)
                     /gene="rlmN"
                     /locus_tag="PLT63_02565"
     CDS             complement(821..1852)
                     /gene="rlmN"
                     /locus_tag="PLT63_02565"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOD97633.1"
                     /translation="MDKINIRDLSPGEIEAFIAHLGKEKYRARQLMKWLYHFNASSFG
                     EMTTLSRELRGHLEETASLDGPEIAKIRTAADGTKKVLFRLRDGLHIESVLIPGRNHW
                     TACLSSQAGCAMGCRFCFTRSLGFQRQLTPGEITGQLTALKFRTPEGPNVKNVVLMGM
                     GEPLANYEHTVTAVRIMTDDAGMGWSNRKVTISTCGLAPQIRRLGRDDICVNLAISLN
                     APDNERRDYLMPVNRRYPIETLLAACRDYPMPGRRMLTFEYILMAGVNDSPADAEKVA
                     RLLKNLHCKLNLIVFNEFPGAEFRASSPEAVAAFQQVLLRHHYTSIIRASRGSDILAA
                     CGQLTGNAA"
     gene            complement(1852..3078)
                     /locus_tag="PLT63_02570"
     CDS             complement(1852..3078)
                     /locus_tag="PLT63_02570"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HOD97634.1"
                     /translation="MNKKQKIIVKTPLVEMDGDEMTRIMWQFVKELLILPYLDIELDY
                     FDLHIKHRDDTNDQVTVDAARAIMKYGVGVKCATITPTADRVREYNLKEQWKSPNGTI
                     RALLDGTVFRTPIFAKNIHPFVTTWKRPIIIGRHAYGDVYSNAELRIPGAGRAEIVFT
                     PDGGGAVLSNTIHDFTGPGIIQGIHNTDASIAGFATACFTYALDRKVDLWFSTKDTIS
                     KTYDARFRAIFDAEFAARWREKFAAAGIEYFFTLIDDAVARIVRSEGGILWALKNYDG
                     DVMSDMIAAANGSLAMMTSVLVSPRGHFEYEAAHGTVQKHYYQHLAGKETSTNSMALI
                     FAWTGALRKRGELDGSPEVSSFADQVEAAALETVEAGIMTGDLLTVAEPAPHNRKVET
                     RAFIEAVAGRLEEKLH"
     gene            complement(3148..5079)
                     /locus_tag="PLT63_02575"
     CDS             complement(3148..5079)
                     /locus_tag="PLT63_02575"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HOD97635.1"
                     /translation="MGKTIIEKIIETHRETAPESPAGEIGIRIDQTLTQDATGTMAYL
                     QFEAMDLPEIRTELSVSYIDHNTIQIGYENADDHRYLQSVAGRFGIHLSRAGNGICHQ
                     VHLERFGIPGKTLIGSDSHTPTAGALAMLAVGAGGLDVALAMAGRPFYLPAPRIFRIE
                     LQGKLPPWVAAKDVILKILSIFTTKGNVGRIFEYSGSGLAFLTVPERATIANMGAECG
                     VTTSIFPSDDMTRRFLAAQGRAKDWIPLHADADAVYEGEAVIDLSRLEPLAARPHSPD
                     RISPVREIGPLAIRQVCIGSCTNSSYKDLATVAEILKGRTVHPEVSLIVAPGSRQVLE
                     NITREGYLAHILGAGARLMENACGFCIGNSQSPPSGAVSLRTSNRNFYGRSGTRDADI
                     YLVSPETAAVSALKGRLTDPRQLGIALPFIPQPRKFHLDDNMIIRPLPPEDRAGVEII
                     RGPNIGAPPDNNPLPSHIDGEVAIKVGDKITTDHIIPAGARMKYRSNIPKYAHYVFEI
                     LDRDFSARALRIRNEGRQNIIVAGHSYGQGSSREHAALCPMYLGVKVVLAKSFERIHA
                     ANLINFGILPLIFENEGDYELLQAGDRLRVTDIRKVLTERRPLRIDNLTRGGYLPAAY
                     ELTDRQLALILGGGALRAR"
     gene            complement(5177..5929)
                     /locus_tag="PLT63_02580"
     CDS             complement(5177..5929)
                     /locus_tag="PLT63_02580"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOD97636.1"
                     /translation="MSRTLYLALIALLASAFMSGCVAKSDYLIKVAEAEARTQELKTC
                     NLEKEKLLAQRTELEKVLKTEENAARQKVADLQHENAKLKESVAALQKKEEIIQKESD
                     TYRDLLREMKGEIAKGQVTITELKGKLTVDVVDKILFASGDAKVKKEGLDVLKRVVDI
                     LKNLKDKAIIVEGHTDNVQIRGSLAKVYPTNWELSAARAINVARYLQQQGINPELLKA
                     VACGEYRPIADNGTPEGRQKNRRIAIVLSAKD"
     gene            complement(5951..6934)
                     /locus_tag="PLT63_02585"
     CDS             complement(5951..6934)
                     /locus_tag="PLT63_02585"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HOD97637.1"
                     /translation="MMERRIFLWMGLIVLFFSLLFGTDGFADQQYRVKRGDSLARIAK
                     KYGVTSQSIREANGIEGNRIKAGKLIVIPTNSSQKLAKSSKTKPQRTDRLYVVKKGDT
                     LARIARKSGLTVAQLKHLNHLKSNKLRNGQKLALAKPAAEGPGPRVSKAEPTRNDPAE
                     AEDDLLSDEIAPAEETAAPENEIRYSESTLGKWKDPEERSLLVKVAKGFLGAPYRLGG
                     SSVKGLDCSGFVKKMYEFFDVNLPRTAREQAHVGMKVAKGELAEGDLVFFNTRRAFGH
                     VGIYIGNNQFVHASSGKKDRHVRIDSLDKPYYNKRFVKAVRLKSLEEDNNI"
     gene            7219..8106
                     /gene="mtgA"
                     /locus_tag="PLT63_02590"
     CDS             7219..8106
                     /gene="mtgA"
                     /locus_tag="PLT63_02590"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HOD97638.1"
                     /translation="MGKKLFILAAVIFFSGMFINIGSYFLFPDVAGLAKKNPTKTAFM
                     EYREREWQREGKKKKIQQRWVSLIRISPYVIKAVIIAEDDKFWGHEGFDFEAMQKALE
                     KDIQKGKFKAGGSTISQQLAKNLYLSPSKNPVRKLKEAILTWRIEKRLSKKRIIELYL
                     NVAEWGDGIFGIEMAARAHFGKTAADLSAQEAARLAAVLPNPRKFNPAGASRYVVSRS
                     ERIYRIMVKRGIVIPEYEDVMKEQSDLGSLLESREPEAIKKLEQKVEEWKKEGADAGG
                     GTQTPTTAPGDRNGGVDRD"
     gene            8166..9365
                     /locus_tag="PLT63_02595"
     CDS             8166..9365
                     /locus_tag="PLT63_02595"
                     /inference="COORDINATES: protein motif:HMM:NF012481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HOD97639.1"
                     /translation="MADIYKAVKLTDKVYWVGAIDWTIRDFHGYTTHRGSTYNAYLIL
                     ADKITLVDTVKAPFREELISRIASVVDPGDIEIIVSNHSEMDHTGCLYELIDLIQPEQ
                     VVASTMGTKTLGDIYHRERTITPVKDGGELSLGNMRLRFMETRMLHWPDSMFSYLPEE
                     EILFSQDAFGMHLATAERFDDEIDPAILDYEGATYFANIILPYAPLVTKLVNRVASSG
                     MTFKFIAPDHGPIWRRDIPGVIANYSRWAAQKPTMKSVVVYGTMWHSTERMARAICEG
                     LAAAGAAAKCMSMEVFHRSDVVYEVLDAGALIVGSSTLNNNMLPQVADVLTYLKGLRP
                     ANLLGASFGSYGWSGEAAVHIADILKEMKVSPVAEPLKAQHVPTREDLRKCYALGEEI
                     ARKLGEK"
     gene            9375..10106
                     /locus_tag="PLT63_02600"
     CDS             9375..10106
                     /locus_tag="PLT63_02600"
                     /inference="COORDINATES: protein motif:HMM:NF012275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemopexin repeat-containing protein"
                     /protein_id="HOD97640.1"
                     /translation="MKTSKGATIVIGALFLLFVGAFAAHGETYIPQKAIGAGANWGNG
                     KVYFFKGDHFVRYSARPDLEEDLMGYRADPGYPKAIDKTTWPGMIWKDIDAVINMGNG
                     KACFFKGGQFIRYDIKADRADPGFPQAINEQTWPGLPWTDGIDAAVYWGNGKIFFFKG
                     GQYVRYDVEKNRTDSGYPKSINKRNWPGMIWTSGIDDVIFWGNGKAYFFKDYEYIRYD
                     VNEDRADPGYPKQVNRQTWPGLVNW"
     gene            10227..10385
                     /locus_tag="PLT63_02605"
     CDS             10227..10385
                     /locus_tag="PLT63_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOD97641.1"
                     /translation="MMKYECGICGYVYDPALGDEEGGIAPGTAFEDLPEDWTCPVCGA
                     AKDEFSPA"
     gene            10688..11182
                     /locus_tag="PLT63_02610"
     CDS             10688..11182
                     /locus_tag="PLT63_02610"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOD97642.1"
                     /translation="MSELNKALYEAYVGEAKAALRLKVYADKAEKEGYPQIAKLFRVI
                     SFSEEIHGTRALKLLKEVKSTEENLAASFESEQKVAGVAYDEFIKLAEKAGNKAAVLH
                     FSQSRDVEEVHAKLYKEAMTHFLEARETTYYVCKVCGYVSDGVLPEECPFCGAKKEMF
                     DRFG"
     gene            11279..11629
                     /locus_tag="PLT63_02615"
     CDS             11279..11629
                     /locus_tag="PLT63_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HOD97643.1"
                     /translation="MKDLIVRWLILTVAILAAAYFIEGIHVRGFSSALFAAAFLGVLN
                     ALFRPILIILTLPVNILTLGLFTFVINALLLKMTAGVIPGFDLQGFWPAVFGALIISI
                     VNWLLHLLISDSGK"
     gene            11626..14298
                     /gene="mutS"
                     /locus_tag="PLT63_02620"
     CDS             11626..14298
                     /gene="mutS"
                     /locus_tag="PLT63_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOD97644.1"
                     /translation="MKGGNSERVRTADPVKDLTPAMKQYWELKARYPDCILFFRMGDF
                     YEMFFEDAVVAARLLEITLTSRSKTKEGSIPLCGVPYHAASAYIARLLEKGYKVAVCE
                     QLEDPKQAKGIVKRDVVRVVTPGLRFDPDHLEARENNFLAGLCRLEGVFGLACMDIST
                     GEFRVMEIQDREILQAELAGLSLRELIVPEDFEERQSLTILDKSEAPAAVNTFSGDYF
                     DPEAARSLLESYFPEAELTALEMEKRPAVAGAAGAVLRYGEETQRERLAHVNRIQEMP
                     WSQSLFLDETAKNTLELFQTIQERKKKGSLFSVLDETVTSLGARRLRWWLNHPLRGPE
                     KIKRRLAVVAEIKDNHLLREELRRLLGGVYDLERLGGRIAMDVAHARDLVALKKSLQA
                     IPGIKELLASLTSPLARSLRERLNPLPAVADLIETAIVDDPPLTIREGGMIKDGFDAE
                     LDELLSLSRHGKRNIAALEDRERRKTGINSLKVGFNQVFGYYIEVTKANTHLVPPEYI
                     RKQTLVNAERYINEELKEYEQAVLHAEENSRRREYELFLMIRGDIARSIRPIQDTAAA
                     LAALDALASLASVAESYGYCCPVVDDSDAIEITDGRHPVVERMALSEGFVPNDTCLDL
                     RENRFLVLTGPNMAGKSTYIRQVALIVIMAQMGGFVPAQQARIGVVDRIFTRIGAADS
                     LARGQSTFMVEMREMANILNHATRRSLVILDEVGRGTSTFDGLSIAWAMAEYLHDERH
                     IGARTLFATHYHQLTELALIHEGVKNYNVAVREWGDRVIFLRKIMAGGTSRSYGIQVA
                     RLAGVPEEVVRRSGEILKNLERGELDAAGSPRIGGGKKGGDKSRAAQQLSLFPDEREE
                     IITGLKELDTTRLTPLAALNLLQEWKDRLIRGSV"
     gene            14313..15239
                     /locus_tag="PLT63_02625"
     CDS             14313..15239
                     /locus_tag="PLT63_02625"
                     /inference="COORDINATES: protein motif:HMM:NF024798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HOD97645.1"
                     /translation="MKRNKWVVFSRTALVLAMSLLFPFSAAAGEWDSLEKKLSADGFD
                     GKEMAALFQRADLRFDPEPMTTKITELMVRDNRQDSGQKKSNRTAVYGGYLRPETLRS
                     AHAYLDRHRVLLGRISRKYCVPREVVVAIALVETNLGANTGRRRAFDVLASMALADDL
                     EKVRPFLPPGVVHGGNEEQARRRCREKAAWAYRELKYLLKYSRATGVDPLAVPGSIYG
                     AIGLCQFMPSNVSLYGIDGDGSGSIDLFTTPDALHSIGNYLHRNGWTCRMAGPQRWRV
                     VMTYNHSQVYANTVLAVADRLRAMDHKKKRKN"
     gene            complement(15257..17560)
                     /locus_tag="PLT63_02630"
     CDS             complement(15257..17560)
                     /locus_tag="PLT63_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HOD97646.1"
                     /translation="MADLATKQINELEEQLQRRKKLQDITNRIHSAQNIKQILVDLKD
                     GILNLFDAHSITIYIVDRIRNEIFSMFLSGSKLSEIRVPINNRSIAGYVANNGKIVNI
                     ADAYDEKELRAIDKELSFDSSWDKKSGFRTRQILTAPIFHNGKLMGAVQILNKKSTVN
                     GGRFSEDEKGFLNEITEVLGVAFFNQERFARRRKTRFDYLISRDLLKEEDLENAWEES
                     REQKETMENFLMKKYKISKENIGKSLAEFYRCKFVQFNDKIPIPGDLIKNLKKDYLRR
                     ELWVPINRLEDGNINILVDDPNNILKRDMIENLMKTKTIRYDVALSDDIVKFINYFYQ
                     SPEDETSISDILGKLDADAGDAEPEEEGEVVTESDSAIMQLVNKIINDAYSRRTSDIH
                     IEPNVGKKNVEVRFRVDGDCGLYQTVPYSYRAALVSRIKIMSNLDITIKRIPQDGKIK
                     FKRPGGDEIELRVATIPTQGGVEDVVMRILAKGETLPLEAMGMLPRNYKGLIDICEKP
                     YGMILVVGPTGSGKTTTLHAALHHINTPDRKIWTAEDPVEITQYGLRQVQVQPKIGFD
                     FAAAMRAFLRADPDVIMVGEMRDFETAKTGVEASLTGHLVFSTLHTNSAPETITRLLD
                     MGIDPLNFADALLGILAQRLVRTLCKNCREAYHPSPKEFEELIESYGEEDFERLNVKY
                     TDDFTLYRPKGCDICDKSGYKGRMGIHELLVASDNIKRMIQKHEPVEVLREMSMKEGM
                     TTLLQDGIQKALQGVTDFKQVRRVCIK"
     gene            complement(17573..19645)
                     /gene="glyS"
                     /locus_tag="PLT63_02635"
     CDS             complement(17573..19645)
                     /gene="glyS"
                     /locus_tag="PLT63_02635"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HOD97647.1"
                     /translation="MGKELLLEIGTEEIPAAFLPRAMADMDELIRKALNGERVKFGPV
                     TTMATPRRLLLHVADVAVTQEDQVIEKIGPTRKAAFDEGGNPTKAAVGFARGQGIDLG
                     EIEIIATDKGEYLCARKRIAGGDVAAMLPGLLAKFIMTIPFKKAMRWSNLELRFARPI
                     HWLLALYGGEVIPVRIENIEAGNASRGHRFMSPGPFAVSDFRDYLDKAREAWVIIDPE
                     ERKKIIREEAQRVAAAVNGQPLITDDLLETVAFLVEYPTAVCGVFDPDYLKLPREVLT
                     TTMMSHQKYFPVVDAAGRLLPHFITINNTPARDPSVVTRGNEKVIRARLSDARFFFEE
                     DQKISLDRRFEELRKVVYHSLLGTSYEKVLRFRKLAALITAKIDPALAAPVDRAAVLA
                     KADLDTQMVGEFSELQGVMGREYALLAGEDPLIAKAIYEHYLPLAAGGELPESDIGAI
                     VGIADKMDTIIGFFGVNLIPSGTADPYALRRQALGIINIILAKGYPLRLAELVAAGIN
                     ILQDKLKRPAEEIARDVLDFFRTRFENQLIAQGHAYDVVDAVLAIDASDLAEALKKIE
                     AMEAFKNHADFQPLAVACKRVANILKDFRNGEVRPELFREDAESELYRVFQEVRKRAA
                     DHLDACRYEGALTEMARLRAPVDAFFDAVMVMAKEEDIRFNRLSLLEEIAALFRRVAD
                     FSKLVTDF"
     gene            complement(19677..20543)
                     /gene="glyQ"
                     /locus_tag="PLT63_02640"
     CDS             complement(19677..20543)
                     /gene="glyQ"
                     /locus_tag="PLT63_02640"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HOD97648.1"
                     /translation="MTFQELIFSLERYWADQGCVIQQPYDLEVGAGTFNPATFLRALG
                     PEPWNVAYVEPSRRPTDGRYGENPNRLQHYYQYQVIMKPSPLNIQELYLDSLKSFGID
                     PREHDIRFVEDDWESPTVGAWGLGWEVWLDGMEITQFTYFQQVGGMDLRPVCAEITYG
                     IERIAMYIQGIDNVYDLKWNDRVTYGDVHHQGEVEWSVYNFEKADVDTLRKLFDMYEA
                     EGIRVAEMDLVLPTYDCCLKCSHTFNMLNARGAISVAERTSYIGRVRNLARLSAELFI
                     KQREKMGYPLIK"
     gene            complement(20608..21402)
                     /gene="recO"
                     /locus_tag="PLT63_02645"
     CDS             complement(20608..21402)
                     /gene="recO"
                     /locus_tag="PLT63_02645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOD97649.1"
                     /translation="MNARTSHKTRAIVLHTLDYGESDRIATFYTREYGKLKGIAKGAR
                     RSRRRFANALEPFSFTQVIFSRRGSGDTLAMVDQCEIIDHFPVIRSDLEKCVAASYLV
                     ELTGAFTVEGKKNEAVFQLLVDFLALMENGPFRTSLLSFFEIRLLGLAGYEPVLDRCT
                     RCHASLEAGRAFIFSNTRGGLQCSRCEPVPGAVPALSLGTIRSLLHGRTLPVRNLSRL
                     LLSPQADDESRRLLVSFIQHLLGRELKSWQVLRQLRQLGAMNINTC"
     gene            complement(21380..22714)
                     /locus_tag="PLT63_02650"
     CDS             complement(21380..22714)
                     /locus_tag="PLT63_02650"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOD97650.1"
                     /translation="MKSAVAGQNWLLRGARVVDPGRGLDDRLDIIVEGGRIAGIGRDL
                     GKKRGKGLRIMELGGLVITPGLVDMHVHFREPGYEYKETIATGSAAAAAGGFTAVACM
                     PNTNPVNDNRSVTEFILRKAGEADLVHVYPIAAITLGSQGNTLAEFWDLKEAGAVALS
                     DDGKPVMDGAIMRRALEYAHSLGMPVVSHCEDLSLSAGGAMHEGLAATELGLPGIPGI
                     AEEVMVARDLLLAEYTGAVLHICHVSSAGSVRLIRDAKSRGVKVTAETAPHYFTLTAD
                     CLRGYDVNAKVNPPLREQRDIEAVREGLRDGTIDVIASDHAPHAVTDKEVEFEYAASG
                     MIGLETSLALSLRLVTDGFLSLPELVTRMSARPVAILGVPGGSLAPGAPADLTVIDLE
                     RRWTVSPEGIRSRSRNTPFLGWHLTGKAVLTMKGGAVTHSDLLRDHERPDQP"
     gene            complement(22847..23770)
                     /locus_tag="PLT63_02655"
     CDS             complement(22847..23770)
                     /locus_tag="PLT63_02655"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041585001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HOD97651.1"
                     /translation="MKWERKDMLGIRDLSREEIILVLDTADSFIEVSTREIKKVPTLR
                     GKTIVNLFYEASTRTRTSFEIAGKRLSADTINISSTTSSVVKGETLIDTARNLEAMNP
                     DVIVIRHSAAGAPHLLAGLVKQSVINAGDGAHEHPTQALLDMMTIRAKKGRIENLRIA
                     IVGDIAHSRVARSNIYGLKTMGAQVAVAGPATMLPRDMEHMGVEVHSSLEKALAGADV
                     VMMLRIQIERQHQNIFPSLREYARHFCLNRQNIALAKQDVLVMHPGPINRGVEIAPEI
                     ADGPFSVILNQVTNGVAVRMALLYLLTGGKG"
     gene            complement(23774..24322)
                     /gene="pyrR"
                     /locus_tag="PLT63_02660"
     CDS             complement(23774..24322)
                     /gene="pyrR"
                     /locus_tag="PLT63_02660"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HOD97652.1"
                     /translation="MKNKKRIVMDAEGIDRSLTRIAYEILEKNKGLNDLVLIGIRTGG
                     VYLAYRLRKKIAAIEGIEVPLGILDITLYRDDLSRINKKPRIGKTEIAFSLDNMKVVL
                     VDDVLFTGRTIRAAMDALIDFGRPRLIQLAVLIDRGHRELPIRADFVGKNLPSSLWED
                     VSVNLTEKNGADEVVIEPETEE"
     gene            24680..25948
                     /locus_tag="PLT63_02665"
     CDS             24680..25948
                     /locus_tag="PLT63_02665"
                     /inference="COORDINATES: protein motif:HMM:NF015413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOD97653.1"
                     /translation="MAVNAEPQVFLFLSEILGQPLIGPEGRPVGKIVDLVATVTEPYP
                     VVTEAMIDREEQRGELVLCRFPEVEEVRLPIHADRTTADDYRPPELRENELLLKDSLL
                     DKQIVDTHGAKVLRVNDLQFLMARRCLHLVHVDVGFRGLMRRSGLEKLVDLFLHTFFD
                     YNLPNQYISWKYVQPISTPDLLRLKITQDRLARIHPADLADIIEDLDVNQRSAVFRSL
                     DVETAAEILEETDPKIQVSLIKDMNAAVASDIIEEMSLSEAADLLGDLPRDKAEGILK
                     EMEQDIAEDVKELLAHPEEEAGGLMTSAVLHFLPETTVEKVLAAIRGEAEDMDFIYYV
                     YVVNEADRLLGMVSLRELLAAPMAARLADLMDERVISVHLDEDKDEIAELFAKYGLMA
                     IPVVDDLGRIKGVIPFKNLLEVVAPHLGKK"
     gene            25974..27260
                     /locus_tag="PLT63_02670"
     CDS             25974..27260
                     /locus_tag="PLT63_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HOD97654.1"
                     /translation="MSERNEEGKKVNIFRRLFQKRFWRSLGLLFALIGPGIITSNVDN
                     DAGGITTYSLAGAQYGLSLLWTLIPITVALIIIQEMCARMGVVSGKGLSDLIRERFGA
                     KITVYLMMVLLLANLGNTISEFAGIAASLEIFGVSKYLSVPVGAFFVWWLVVKGSYKS
                     VEKVFLVACLFYVSYIISGFMGKPDWGEIGIALVTPTLSADPAFLTMAVGVVGTTIAP
                     WMQFYLQSSVVDKGLKAEDYAYTRVDIIVGSLTVNIVAFFIIMLCAVTLFQQGIRIET
                     AKDAAQALAPLAGAYCTYLFAFGLLNASLFAASILPLSTAYTICEAFGWESSLNHKFT
                     EAPQFYGLYSLMIVLGAAIILLPNMPLIGIMYYSQVINGLLLPFVLIFMLLLVNDRRI
                     MGEYVNGRTMNLITWLTVGILILLSLAIVITSLLPA"
     gene            27489..29354
                     /locus_tag="PLT63_02675"
     CDS             27489..29354
                     /locus_tag="PLT63_02675"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOD97655.1"
                     /translation="MGNGLVSLRDQQFVLFEQIGIENLFTKDLYADYSRDVVLMMQGE
                     AEKMAVNVILPTYAEADREGCHIDKNGQVRVPKCFLEVFKKYCAGGWLAPMKSPEVGG
                     QNMPVAVSTCFTEMFASANVAFLLYAGLTLGAGGLVENFGTEEQKKKYMYKLYAGEWA
                     GTMCLTEPGAGSDVGALKTTAKRLPDGKFLITGTKCFISSGDHDLTPNIIHPVLARIE
                     GDPPGTGGISIFIVPKIRVNDDGSLGEPNDVKTGNVEHKMGIKGSATCTLNFGDEGKC
                     IGELLGQERQGMRIMFHMMNEARLEVGIQGLGHASAALEHAVQYAKERIQSTPVWEMK
                     NPDAKAVPIIMHPDVRRDLLWMKAYVEGIRALNLFTAYCIDMSRVGETPEERAKYQGF
                     VELLTPVCKAYSSDMACLVTSKAIDVYGGYGYCQEYPVEQYLRDCKIACLYEGTNGIQ
                     SLDLVGRKLGMNKGMNVMNMMVEIDATIKKAKGSEELAKYAAKLEEAYNALVDLTMTF
                     AALGKSSSFLIPILYASPYLDLFGDVLLGHFLFQGASIAREKLDGIFAAKGADSIGKK
                     RALVHTDPDVAFYEGKIASAKFFALEVLASVKAKCEAIKAGDKTAIEMAIESFPS"
     gene            complement(29507..30121)
                     /gene="lepB"
                     /locus_tag="PLT63_02680"
     CDS             complement(29507..30121)
                     /gene="lepB"
                     /locus_tag="PLT63_02680"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOD97656.1"
                     /translation="MTKKPKSKVQEYAEAIIIAILIAFFIRTFVVQAFKIPSGSMKPT
                     LLIGDHILVSKFIYGVKIPYFRKTLIPVRDPKRGEIIVFIYPEDRSKDFIKRVIGVGG
                     DTIEIRNKKIFLNGMPYEDKYGVYVDGFVIPGAIQPRDNFGPVTVPKGSVFTMGDNRD
                     QSYDSRFWGFVDLKDVMGKAYVIYWSWDKEERSLRWNRLGSLIR"
     gene            complement(30141..30653)
                     /locus_tag="PLT63_02685"
     CDS             complement(30141..30653)
                     /locus_tag="PLT63_02685"
                     /inference="COORDINATES: protein motif:HMM:NF016213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HOD97657.1"
                     /translation="MPTLEIIGLSLFVIFLLLGLFSIIFGLPGAVIIAVVVLAYAAVT
                     GFTVIGWKLLLVIVALAVLAETLEFIIGMHVASQFGLSMKGFWASLIGSMIGAAALTP
                     FFLGFGAIAGIFLGGMAGLLMMEMVHRKRLKMAFRASLGMVLGRVAGICVKGCLAFVM
                     VILALNAVYS"
     gene            complement(30655..31575)
                     /gene="secF"
                     /locus_tag="PLT63_02690"
     CDS             complement(30655..31575)
                     /gene="secF"
                     /locus_tag="PLT63_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HOD97658.1"
                     /translation="MAFVEFIKPDINIDFVGKMKLAFSISMAMIIITFASIAWHGGLN
                     YGIDFAGGTLIQVKFNVPFTIDKIREAFQPLGIENSIIQQFGPQEIVVRVGEGTTDLK
                     SLSGKVEEKLNNTFGQNAVEMRRVEMVGPKVGKDLTKKALLAIFFSWIGILIYVGFRF
                     EFRYAAGGILALIHDTIITIGAFSLLNKEFDLTIVAALLTIIGYSINDTVVIFDRIRE
                     NARKNIKKPLAETINDSINQTLSRTILTAFTVFLVLLSLFFFGGPVIHDFAFALIVGV
                     IVGAYSTVFIASPFVLVWDKWMPSKFRRKK"
     gene            complement(31587..33191)
                     /gene="secD"
                     /locus_tag="PLT63_02695"
     CDS             complement(31587..33191)
                     /gene="secD"
                     /locus_tag="PLT63_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOD97659.1"
                     /translation="MIKSIGIRALIVLAVCLVALFYLTPSVMTDRLPPFWKNNLPADK
                     IHLGLDLQGGMHLVLEVQTDKALEGTIERMANDIKETMMEKKIRFRGIARTQKDSITV
                     EFPDHPAREAFEKILKDNYPDFEPASASQRDGKEIVQIKLKAKRAQDIKKLAMEQGLE
                     TIRNRVDQFGITEPEIIPQGEDRIIVQLPGIRDPGRAKNLIGKTALLEFKLLDEEHSL
                     EEAMRGNIPEGSIIAYGTHLDRESGRRTQVPYLVKNKTLMTGASLETAKVSIADRFGE
                     PYVSLKFNAQGAQDFDRITAENVKKRLAIVLDGVIHSAPVIQERISGGNAQITGTFTM
                     DEAKDLAIVLRAGALPAPVTILEERTVGPSLGQDSIDKGIWSTIAGLMLVILFMMIYY
                     KISGLVANLALILNIIIIFGTLAAFRATLTLPGIAGIVLGIGMAVDANILILERIREE
                     IRGGKSPRASVEAGYTKAFLAIFDSNLTTLIAALFLFGFGTGPVKGFAVTLTIGLIAN
                     MFTAVFVTRIIYDYFIWNRKIQTISV"
     gene            complement(33211..33537)
                     /gene="yajC"
                     /locus_tag="PLT63_02700"
     CDS             complement(33211..33537)
                     /gene="yajC"
                     /locus_tag="PLT63_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOD97660.1"
                     /translation="MIATAYAMGSAGGGKMGGGGDWSFIVVMVILFAIFYFLLIRPQQ
                     QKQKELKTMIDNLAHGDTVLTSGGIHGKVVGLTDAIVTLEIADKVKIKVSRSFISAVL
                     QKAGKE"
     assembly_gap    33703..33802
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(33807..34886)
                     /gene="tgt"
                     /locus_tag="PLT63_02705"
     CDS             complement(33807..34886)
                     /gene="tgt"
                     /locus_tag="PLT63_02705"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOD97661.1"
                     /translation="MNFDFELIKKDEHSGARLARMRTAHGVIYTPAFMPVGTQGAVKA
                     LTPDMVRELGAQIILSNTYHLYLRPGHGIVAAAGGLHKFMNWSGPILTDSGGYQVFSL
                     GALRKITEEGVLFQSHIDGSRHLLTPEAAVDIQLALGADIMMCLDECTPYPATFREAE
                     LSLDRTVRWARRCHHRRGDAAALFGIVQGGMHMELRRRSLEALLEMPFEGLALGGLSV
                     GEPKEEMMAVVGETTPQLPEERPRYIMGVGTPEDIVACVARGADMFDCVMPTRSARHG
                     LLFTSEGKIVIKHARYREDQAPIDELCDCYTCRNYSRAYLRHLFVSGEILGLILNTIH
                     NLRHYLRLMERIREAIAAGRFPGNI"
     gene            complement(34901..35938)
                     /gene="queA"
                     /locus_tag="PLT63_02710"
     CDS             complement(34901..35938)
                     /gene="queA"
                     /locus_tag="PLT63_02710"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF014592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOD97662.1"
                     /translation="MKLEEFSYHLPPSFIAQAPLPHRDRSRMLVLEAATGQVRHRIFR
                     DIPEYLREGDVLVVNDSRVIPARLVGRKESGAVIEILLLSKIPATGEGELWEAMLKPA
                     KRVRVGTVIQLPGAGRAEVKERRSEKIWRLRFFSPEPFAAYLNRHGRPPLPPYIKRTP
                     EDDRSAGDRDRYQTIYARAPGSVAAPTAGFHFSAAVLESIRKRGIGIAAVTLHVGYGT
                     FLPITATDIEDHRMEAEYFEIGAEAAAAINGAARVVAVGTTATRALETVADEGGTVHP
                     TAGWTSLYIYPGYRFKRVRALLTNFHLPRSSLYLLTCAFGGKQAVAAAYAEAIGRDYR
                     FYSYGDCMLII"
     gene            complement(35935..36123)
                     /locus_tag="PLT63_02715"
     CDS             complement(35935..36123)
                     /locus_tag="PLT63_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HOD97663.1"
                     /translation="MKYFLCVLGMVFIIEGLPYFAFPEKLKIYLAKMSEVPDTTLRLM
                     GLAAIIAGLALVYFGTAA"
     gene            36412..>37055
                     /locus_tag="PLT63_02720"
     CDS             36412..>37055
                     /locus_tag="PLT63_02720"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOD97664.1"
                     /translation="MAEEEKKPRLTRAERLEREREKNLQHWLAQDEILFKHREEAMNI
                     GGDAQVERLAKQNKKPMRQLISQLIDPGTEFFEVGLDAGYDIGKKRLYGGEIYESEKR
                     GHIPGGGMVTGVGVVHGKDCMIFANENRYAAGTYFPITLKKHMRAQAIAEASRLPCIY
                     IADSGGINLPLQVGCFADDGHFGSMFYNMCRMSAMGIKQFTLSTGGNTAGGAYIV"
BASE COUNT         7488 a        10410 c        11099 g         7958 t
ORIGIN      
        1 attcccgcca gtggcacgaa gccgttctcg actggctgca ccgttggctt cagggtcagg
       61 acgataaaaa gggccgcgaa gggtgagggc ggagtccata accgttgaga ggtctcaagg
      121 aacagatatt tatggaatac attttcattt tcagcatttt gatcgcttag tttttcagca
      181 atccttttaa cttgaggttc agttcaactg gtttttgcac ggtctttccg gagggacggt
      241 tatatctcgg aatagccgcg aattatctcc tctatttctc gaattatctc ctgggcttca
      301 tcatcatacc ccaatcccca gagggcttca cagaggatta cccggccctc cacatccccg
      361 ggcatttccg caagtcgtgt ttccgccaga tcccgggcga gctgataaag accgcgatcc
      421 aggaaattgc gggcctgctc gggaaactca tctttgcctg ttcgatccgt gtattccata
      481 aatctggctc ttcgggaaaa ggtatccgcc tcaacgcaga accaggcgtt caattcctcg
      541 ttccggaaaa gtccggcgtc accatgtcgg cgattcggcc attcaggaga tgattaaaaa
      601 agccatcaag atggcagcca tcccgaaaca cggatcagta catactctgc gccatagttt
      661 tgcaacgcat ttgctgatga acggcgtcaa tatccgcgag gtacaggaac ttttaggcca
      721 caagaacgtg gaaacaacta tgatttatac ccatgtgatg cgctctattc tgtgcaaaat
      781 cgcatgaaat cgtcaattat tctcgatatc gagttttgat tcaggcggcg tttcccgtca
      841 actggccgca ggcggcaagg atgtcgctgc cgcggctggc gcggataatg ctcgtgtaat
      901 ggtgccgcag cagcacctgt tgaaaggccg ccacggcctc cggggaagag gcacggaatt
      961 ccgcaccggg gaattcgtta aagacgatga ggttcagttt gcaatgtagg ttcttcagga
     1021 gacgggccac cttttccgca tccgccgggc tgtcgttgac gccggccatg aggatatatt
     1081 cgaaggtgag catccgccgc cccggcatgg gatagtcccg gcaggccgca aggagcgtct
     1141 ctatggggta gcgacggttc accggcatga gatagtcccg ccgctcgttg tccggggcgt
     1201 tgagggaaat cgccaggttc acgcagatgt cgtcccggcc gaggcggcgg atctgcgggg
     1261 ccagaccgca ggtggagatc gtcaccttcc ggtttgacca ccccattccc gcatcgtcgg
     1321 tcatgatccg caccgctgtg acggtatgtt cgtagttggc caggggctcc cccatcccca
     1381 tgaggacgac gtttttgacg ttggggcctt cgggggtccg gaacttcagg gccgtcaact
     1441 ggccggtgat ctctcccggg gtgagctggc gctggaaacc cagggacctg gtgaagcaga
     1501 agcggcaccc catggcgcag cccgcctggg aggagaggca ggccgtccag tgattccgac
     1561 cggggatgag gacgctttcg atgtggaggc cgtcccggag gcggaagagg accttctttg
     1621 tgccgtccgc tgccgtccgg atcttcgcga tttccggtcc gtcgagggag gcggtctcct
     1681 ccagatgccc ccgaagctcc cgggaaaggg tggtcatttc cccgaaagaa gaggcgttga
     1741 aatggtaaag ccacttcatg agctgccggg cccggtattt ttcctttccc agatgggcga
     1801 tgaatgcttc gatttccccc ggcgacagat ctctaatgtt gatcttgtcc attaatggag
     1861 tttctcttcc aggcgcccgg cgacggcctc gataaaggcc cgggtttcga ccttgcggtt
     1921 gtggggggcg ggctccgcta ccgtcaggag atctccggtc atgatccccg cctccaccgt
     1981 ttccagggcc gcggcctcca cctgatcggc aaaggacgac acctccgggc tcccatccag
     2041 ttcgccccgc ttccgcagtg cccccgtcca ggcgaagatc agggccatgg agttcgtcga
     2101 ggtctccttg cccgcgaggt gctggtagta gtgcttctgc accgtcccat gggcggcttc
     2161 gtattcgaag tgaccccggg gcgaaaccag gacggaggtc atcatggcga ggctgccgtt
     2221 ggcggcggcg atcatgtccg acatgacgtc gccgtcgtag ttcttcagcg cccagaggat
     2281 gcccccttcg gatcggacga tgcgggccac ggcatcgtca atgagggtga aaaaatattc
     2341 tattcccgcc gcggcgaact tttcccgcca gcgggcggcg aattccgcgt cgaagatcgc
     2401 ccggaaccgg gcgtcgtagg tcttggagat ggtgtccttg gtgctgaacc agaggtccac
     2461 tttccggtcc agggcgtagg tgaagcaggc cgtagcaaag ccggcgatgg aggcgtcggt
     2521 gttgtgaatg ccttggatga tgccgggacc ggtgaaatcg tggatggtgt tgctcaggac
     2581 cgcgccgccg ccgtcggggg taaagacgat ctccgcccgg cccgcaccgg ggatgcggag
     2641 ttcggcgttg ctgtaaacgt cgccgtaggc gtgcctgccg atgatgatgg gccgcttcca
     2701 ggtggtgacg aagggatgga tgtttttcgc gaagatgggg gtacggaaga cggtcccgtc
     2761 gagaagggcg cggatggtcc cattcgggct cttccactgt tccttcagat tgtattcccg
     2821 gacccggtcc gccgtggggg tgatggtggc gcacttgacc cctacgccgt atttcatgat
     2881 cgcccgggcg gcatcgacgg ttacttggtc attggtgtcg tcgcggtgtt tgatgtggag
     2941 atcgaaataa tccaactcaa tgtccagata gggcaggatg aggagctcct tgacgaattg
     3001 ccacatgatc cgggtcatct cgtcgccgtc catttccacc agcggcgtct tgacgatgat
     3061 tttctgtttt ttgttcatga ggtgcagctc ccagtgtttt cttttttcct gatctccggt
     3121 cggcggatgg gttggcagca gggccggtta ccgggcgcgc agggcgccgc cacccaggat
     3181 gagcgcaagt tgccggtccg tcagctcgta agcggccggg agatagcctc cccgggtgag
     3241 gttgtcgatc cttaacggtc ggcgttccgt cagaaccttc ctgatatccg tgacccggag
     3301 gcggtcgccg gcctggagaa gctcataatc gccttcgttt tcaaaaatca gggggagaat
     3361 gccgaagttg atgaggttgg ccgcatgaat ccgttcgaat gattttgcca gcacaacctt
     3421 gacgcccagg tacatgggac agagggcggc gtgttcccgg gatgagccct ggccgtaaga
     3481 gtggccggcg acgatgatat tctgccggcc ttcgttacgg atccgcaggg cccgagcgga
     3541 aaaatcgcgg tccaagatct cgaagacgta atgggcgtat ttggggatgt tggagcggta
     3601 cttcatccgg gccccggcgg ggatgatgtg atccgtggtg atcttgtcgc ccacctttat
     3661 cgccacctcg ccgtcgatgt ggctgggcag ggggttattg tcgggagggg cgccgatatt
     3721 ggggccccgg atgatttcca ccccggcccg gtcctccggg ggcagggggc ggatgatcat
     3781 gttgtcatcc agatgaaatt tccggggctg ggggataaag ggaagggcaa tccccagttg
     3841 acggggatcc gtgagccgtc ccttgagggc ggagaccgcg gcggtttccg gactgaccag
     3901 atagatgtcc gcatccctgg ttccggagcg gccgtagaaa ttgcggttgg acgtgcgcag
     3961 cgaaacggct ccggagggtg ggctctggct gttgccgata cagaagccgc aggcgttttc
     4021 catgagccgg gcgccggcgc cgaggatatg ggcgagatag ccctcccggg tgatattttc
     4081 cagaacctgg cgggagccgg gggcgacgat gaggctgact tcgggatgga cggttctacc
     4141 cttcagaatc tccgccacgg tggccaggtc tttgtacgac gaattggtgc agctcccgat
     4201 gcatacctgc cggatggcga gcggacctat ttcccggacc gggctgatgc ggtcggggct
     4261 gtggggccgg gcggccaaag gctccaggcg ggatagatcg attacggcct ccccctcata
     4321 gacggcatcg gcatcggcgt ggaggggaat ccagtccttg gcgcgtccct gggccgccag
     4381 gaaacgccgg gtcatgtcgt cgctggggaa tatcgaggtg gtcacgccgc attccgcgcc
     4441 catgttggcg atggtggccc gttccgggac cgtcaggaac gccagcccgc tgccgctgta
     4501 ttcgaagatc cgccccacgt tgcctttggt cgtgaagatg ctgaggatct tgaggatgac
     4561 gtccttggct gcaacccagg ggggcagctt gccttggagt tcaatccgga atattctggg
     4621 ggcgggcaga taaaagggcc tgccggccat ggccagggct acgtccaggc cgccggcgcc
     4681 cacggcgagc atggccaggg ccccggccgt cggcgtatga ctgtcggaac cgatcagggt
     4741 ctttcccggg atgccgaagc gttccagatg gacttgatgg cagatcccgt tgcccgcccg
     4801 ggagaggtga attccgaacc gtccggcgac gctctgaaga tagcggtggt cgtcggcatt
     4861 ttcatagccg atctgaatcg tgttgtgatc gatgtaactt accgacaact cggtacggat
     4921 ttccgggaga tccatggcct cgaactgcag ataggccatg gtccccgtgg catcctgggt
     4981 gagggtctga tcgatgcgga tgccgatctc cccggcgggg ctttcaggag cggtttcccg
     5041 atgagtttcg ataatctttt caatgatggt ttttcccata attatcaatt gttggccttc
     5101 ccacggccac taatgaaaag ggcacggcgt tgaacggccg tgcccgcatt tgttatggat
     5161 agttaaattt ccttcgttaa tccttcgcgg aaaggacgat ggcgattctc cggttcttct
     5221 gtctcccttc cggggtgccg ttgtccgcga taggtctgta ttccccgcag gctaccgcct
     5281 tcagcagttc ggggttgatg ccctgctgct ggaggtatct ggcgacattg atggctcggg
     5341 cggcggagag ctcccagttc gtgggataga ctttcgccag ggatccgcgg atctggacgt
     5401 tgtcggtatg tccctccacg atgatcgctt tatccttgag attcttgagg atgtccacca
     5461 cccgcttcaa cacgtccagg ccttccttct tcactttggc gtcgccagag gcaaagagga
     5521 tcttgtccac cacatccaca gttaattttc ccttcagttc cgtaatggtg acctgacctt
     5581 tggcgatttc tcccttcatc tcccggagaa gatcacggta ggtgtcgctc tctttctgga
     5641 tgatctcctc tttcttctgc agggcggcca ccgattcctt cagcttggca ttttcgtgct
     5701 gcagatccgc gactttctgc cgggcagcgt tttcctccgt tttcagcacc ttctccagtt
     5761 ccgtgcgctg ggcgaggagt ttttcctttt ccaggttgca ggtcttcaat tcctgcgtcc
     5821 gggcctcggc ctcggccacc ttaatcaggt aatcgctctt cgccacgcaa ccgctcatga
     5881 acgcggaggc cagcagggca ataagggcca ggtagagagt tctactcata gattccttct
     5941 tcgtaaccga tcaaatattg ttgtcttctt ccagggattt gagccgtact gcctttacga
     6001 agcgcttgtt gtaatagggc ttgtccagac tgtcgatccg gacatggcgg tcctttttcc
     6061 ccgacgaagc gtgtacgaac tggttgttac cgatatagat gcctacatgt ccgaaggccc
     6121 ggcgggtatt gaagaatacc aggtcacctt cagcgagctc gcccttggcg accttcatcc
     6181 ctacatgggc ctgctccctt gccgttcgcg gcagattgac atcgaagaat tcatacatct
     6241 tcttcacaaa tccggaacag tccaggcctt tcacgctgga gccgcccagc cggtaggggg
     6301 cgcccagaaa ccccttggcg accttgacca ggaggctgcg ttcctccggg tctttccact
     6361 tccccaacgt gctttccgaa tagcggattt cgttttccgg cgccgctgtt tcttccgccg
     6421 gggcaatttc atcgctgagc agatcatctt ccgcttccgc tgggtcgttc cgggtcggct
     6481 ctgccttact gacccttggt cccggaccct cggcggctgg tttggccagt gccagcttct
     6541 ggccgttccg cagcttgttg ctcttgagat gattcagatg cttgagttgg gcaacggtta
     6601 ggcccgattt tctggcgatc ctggccaggg tgtcgccctt tttcacgacg taaaggcgat
     6661 cggttctttg cggcttcgtt tttgaactct tggcgagctt ctgggagcta ttggttggga
     6721 tgacgatcag ttttccggcc ttgatgcggt tgccctcgat gccgttagct tctctgatgc
     6781 tctggctcgt gactccgtac ttcttggcga tccgggcgag gctgtcgccc cgttttaccc
     6841 ggtattgctg atcggcaaac ccatcggttc cgaaaagcaa tgaaaagaac aggacaatca
     6901 gtcccatcca caggaaaatc cgccgttcca tcatgagtac ctcctcattg ttattcgggg
     6961 gtcgccccat ttattaacgg tgcacagatt ttgacgatgg atgacaaact gtacacaaga
     7021 aacgattcgc tctcttacaa aaaaaaaccc gtcaagtcaa aaaaaatttt cgctctgcaa
     7081 gcagtcgaac tcgcaaagag cggtcaaaca gcctttgcga aaattaggtc ttaataatta
     7141 ccgtaacata cgcaactgaa ttttaaagaa tatgtccaat atcttgaaaa atctcatgtg
     7201 gtgatttatg aagggggcat gggaaaaaaa ttgtttatcc tggccgcggt cattttcttc
     7261 agtgggatgt ttataaatat cggatcctac ttcctgtttc ccgatgtggc cggcctggcc
     7321 aaaaagaatc ccaccaagac cgccttcatg gaatatcggg agcgggaatg gcagcgggaa
     7381 gggaagaaga aaaagatcca gcagcgctgg gtttccctga tccggatatc gccctatgtg
     7441 atcaaggcgg tgatcatcgc cgaagacgac aagttctggg gccatgaagg cttcgatttc
     7501 gaggccatgc agaaagccct ggaaaaggac atccaaaagg gtaagttcaa ggccggtgga
     7561 agcaccatca gccagcagct tgccaagaat ctgtacctat ccccatcgaa gaatcccgtc
     7621 cggaagctca aggaggcgat cctgacctgg cggatcgaga aacggctgtc caaaaagcgt
     7681 atcattgaat tgtatctgaa tgtcgccgag tggggtgacg ggatcttcgg catcgaaatg
     7741 gccgcccgcg cccacttcgg aaaaaccgcc gcggatctca gcgcccagga ggccgcccga
     7801 ctcgcggccg tgctccccaa cccgcggaag ttcaatcccg cgggcgcctc ccgttatgtc
     7861 gtgtcgcggt ccgagcggat ctacaggatt atggtcaaaa gaggcatcgt catcccggaa
     7921 tacgaagacg tcatgaagga acagtcggat ttgggatcgc tgctggaaag ccgggagccg
     7981 gaggccataa aaaaactgga gcaaaaggtg gaagagtgga aaaaggaggg cgccgatgcc
     8041 ggcggcggga cccaaacgcc cacaacggcg ccgggggaca gaaacggggg ggttgatagg
     8101 gattgacacg ataatggtct aatgttacaa acaccggtaa tgatgcgatc ggcaaaggag
     8161 gacacatggc agacatctac aaggcagtaa aactgacgga caaggtttac tgggtagggg
     8221 ccattgattg gactatccgg gattttcacg gttataccac ccatcggggc agtacctaca
     8281 atgcctatct gattcttgcc gacaagatca cgctggtgga caccgtcaag gccccattcc
     8341 gggaggaatt gatcagccgc atcgcctccg tggtagatcc cggggacatc gagataatcg
     8401 tttccaacca ctcggagatg gaccataccg gctgtcttta cgagctgata gatctcatcc
     8461 aacccgagca ggtggtggct tcgaccatgg ggaccaagac cctgggcgat atctaccacc
     8521 gggaacggac cataactccg gtcaaggacg gcggggaatt gagcctgggt aacatgcgcc
     8581 tccgctttat ggagacccgg atgctccatt ggcccgacag catgttcagc tatctgcctg
     8641 aagaggagat ccttttttcc caggacgcct tcgggatgca cctggcgacg gccgagcgct
     8701 tcgacgacga gatcgatccc gccatcctcg attacgaggg cgccacctat tttgccaaca
     8761 tcatccttcc ctatgccccg ctggtcacga agttggtgaa ccgggtggca tcttccggga
     8821 tgaccttcaa gttcattgcc ccggatcatg ggccgatctg gcgccgggac attccggggg
     8881 tcatcgccaa ttatagccgg tgggcggctc aaaaacccac catgaaaagc gtggtggttt
     8941 acggcaccat gtggcacagt acggaaagaa tggcccgggc catctgtgaa ggcctcgccg
     9001 ccgccggcgc ggcggccaaa tgcatgtcca tggaagtatt tcatcgcagt gacgtcgtct
     9061 acgaggtcct ggatgccggc gccctgatcg tgggctcctc gaccctcaac aacaacatgc
     9121 tgccccaggt cgccgatgtc ctgacctatc tgaagggact ccgcccggcc aaccttctcg
     9181 gggcgtcctt tggatcgtac ggatggagcg gcgaggcggc ggtgcacatt gcggacattc
     9241 tgaaggagat gaaggtctcg cccgtggcgg agcccctgaa ggctcaacac gtgcccacga
     9301 gggaggatct gcggaagtgt tacgccctgg gagaggagat cgcacgaaag ttgggagaga
     9361 aataaggagg ttgcatgaaa acgtccaagg gagcgaccat tgtcataggg gcattgttct
     9421 tgttgtttgt cggggccttt gccgcccacg gggagacgta tattccccaa aaggcgatcg
     9481 gtgccggcgc gaattggggt aacggcaagg tctatttttt caagggagat catttcgtgc
     9541 ggtattccgc ccgcccggac ctcgaggagg acctcatggg ctaccgcgcc gaccccggtt
     9601 atcctaaagc catcgataag accacctggc cgggtatgat ctggaaagat atcgatgccg
     9661 tgatcaacat gggcaacggg aaggcctgtt ttttcaaggg cgggcaattc atccgctacg
     9721 acatcaaggc cgaccgggcc gatccgggct ttccccaggc gatcaacgag cagacctggc
     9781 cgggactgcc atggacggac ggtatcgatg cggcggtgta ctggggtaac ggcaagatat
     9841 tctttttcaa gggcgggcag tatgtgcgct atgacgtgga aaaaaaccgc accgattcag
     9901 gataccccaa atccatcaac aagaggaatt ggccgggaat gatctggacc agcggcatcg
     9961 acgatgtcat cttctggggc aatggtaaag cctatttctt caaggattac gagtatattc
    10021 gctatgatgt caatgaggac cgggccgatc ccggttatcc gaaacaggtg aacaggcaga
    10081 cctggccggg tttggtgaat tggtgacggc ggtaagatcg ggagaatgcc catcgcaggc
    10141 atcgcgcctc cacgtctgga acgggcctgg cgagatttgc ctttcggcat ctaccggcac
    10201 gacaaatttg ataaaggagt gaggagatca tgaaatacga atgcggcatc tgtggttatg
    10261 tctatgaccc ggcgctggga gatgaagagg ggggaatagc tccgggaacg gcattcgaag
    10321 accttcccga ggactggacc tgcccggtct gcggggcggc caaggatgaa ttttctccgg
    10381 cgtagcatcc caaggggaat tttgatgcgt ccgtataaag tcgcaaatca ccttcccctt
    10441 caaggggagg accggggtgg ggatggtttt tattaagcaa ttataaaatg attaccccat
    10501 ccccctcctg atctccccct tgaagggggg agggataaat gacttacttc gaaaatagag
    10561 cgaaatgcta aataaaatga aaggtaacat atttttcatg ctttgtgatg ctccacgggg
    10621 catgagggta ttttgcgtgg tcgtcaattt aacgcatgtt taaccatgga gatgaggagc
    10681 tgccaagatg agcgaactga acaaggccct ctatgaggct tatgtggggg aagcgaaagc
    10741 ggccctgcgc ttgaaggtat atgcggacaa ggcggaaaag gaaggatacc cccagatcgc
    10801 caaattgttt cgtgtgatct ctttttccga ggagatccac ggcacccggg cccttaagct
    10861 gctcaaggaa gtgaaatcga cggaggaaaa tcttgcggcc agtttcgagt cggaacagaa
    10921 ggtggccggc gtcgcctatg acgagtttat caagctggcg gaaaaggcgg gcaacaaagc
    10981 ggcggtgctg catttcagcc agagccggga tgtggaggag gtccatgcca agctctacaa
    11041 agaggcgatg acccattttt tggaggcccg tgagacgacc tattatgtct gtaaggtctg
    11101 tggatatgta tccgacggtg tccttcccga agaatgcccg ttctgcggcg ccaaaaagga
    11161 aatgttcgat cgtttcggtt agcgggtttt aatcagtctc aacagtggac aaccccccgg
    11221 atcgcggtcg ggccggcggt gggatcggca tctccagtcc atgaggtaat ctattcaaat
    11281 gaaggatctc atcgttcggt ggttgatatt gaccgttgcc atcctggccg ccgcctattt
    11341 tatcgagggc atccacgtcc gtggtttctc cagtgccctt ttcgcggcgg cgtttctcgg
    11401 ggtactcaac gccctgttcc ggccgatact catcatcctt accctgcccg tcaacattct
    11461 caccctgggg ctgttcacct ttgtcatcaa cgccctgctc ctgaagatga cggccggcgt
    11521 aattcctggc ttcgatctgc agggattctg gcccgccgtg ttcggggcgc tgatcatcag
    11581 catcgtcaac tggcttctcc atctcctgat ttccgattcg ggtaaatgaa gggcgggaat
    11641 tccgagcggg tccggacggc ggatccggtg aaagacctta cgccggcgat gaagcagtat
    11701 tgggagttga aggcccgcta tcccgactgc atcctcttct tccggatggg ggatttctat
    11761 gagatgttct tcgaagacgc cgtcgttgcc gccaggctcc tggagataac cctcacttcc
    11821 cgcagcaaga ccaaagaggg aagcatcccc ctctgcgggg ttccctatca cgccgcctcc
    11881 gcctatattg cccggctgct cgaaaaggga tacaaggtag ccgtctgcga gcagctcgaa
    11941 gatccgaaac aggccaaggg gattgtaaaa agagacgtgg ttcgggtggt gacgccgggg
    12001 ctgaggttcg atcctgatca tctcgaggcc cgggaaaata attttctcgc gggcctctgc
    12061 cggctggaag gggtcttcgg actggcctgc atggatattt ccaccggcga attccgggtg
    12121 atggagatac aggaccggga aatcctgcag gcggaattgg cggggctgag tctgcgggaa
    12181 ctcattgtcc ccgaagattt cgaggagcga cagtccctaa caattttgga taaaagcgag
    12241 gctccggcgg cggtcaatac cttttccgga gactacttcg atcccgaagc cgcccggagc
    12301 ctcctggaga gctattttcc cgaagcggag cttaccgccc tggaaatgga aaagcggccc
    12361 gccgtcgccg gcgccgccgg cgcggtcctg cgttacggag aggaaaccca gcgggaacgt
    12421 ctggcccatg tgaaccgtat tcaggagatg ccctggtcgc agtccctctt cctcgacgaa
    12481 acggcaaaga ataccctgga gctgttccag accattcagg agcggaagaa gaagggatcc
    12541 ctgttttccg tactcgacga gaccgttacc tccctggggg ccagacgcct gcgctggtgg
    12601 ctcaaccacc ccttacgcgg cccggagaag atcaagcgcc ggctcgctgt ggtggcggaa
    12661 atcaaggaca accacctgct gcgggaagaa ctccgccggc ttctgggggg tgtttatgat
    12721 ctggagcggc tgggcggacg cattgccatg gatgtggccc atgcccggga tctcgttgcc
    12781 ctgaagaagt ctcttcaggc cattccgggg atcaaggaac tgctggcatc gctgacttca
    12841 ccgctggcaa gatcgttgcg ggagcggctg aatcccctgc cggcggtggc ggacctcatc
    12901 gaaacggcca tcgtagatga cccacccctg acaatccgcg agggagggat gatcaaggac
    12961 ggctttgacg ccgaactcga cgaactgctg tccctttccc gccacggcaa acggaacatc
    13021 gccgccctgg aagaccggga acgacggaag acggggataa attccctcaa ggtgggcttc
    13081 aatcaggtat tcggttatta cattgaggtc accaaggcca acacccacct cgttcccccc
    13141 gaatatatcc gcaagcagac cctggtcaac gccgagcggt atatcaacga agaactgaag
    13201 gaatatgaac aggcggtcct ccatgcggaa gaaaacagcc ggcgccgtga atatgagctg
    13261 ttcctgatga ttcggggcga catcgcccgg tccatccggc cgatccagga tacggcggcg
    13321 gcgctggccg ccctggatgc cctggcctcc ctggcctccg ttgcggagag ctacggctac
    13381 tgctgtcccg tcgtcgatga cagcgacgcc atcgaaatca ccgacggccg ccatccggtg
    13441 gtggaacgca tggccctctc cgagggattc gtgcccaacg acacctgcct cgacctacgg
    13501 gaaaaccgtt tcctggtcct gaccggaccc aatatggctg gcaaatccac ctacatccgc
    13561 caggtcgccc tgatcgtcat catggctcag atgggaggct tcgtcccggc gcagcaggcc
    13621 cggatcgggg tcgtggaccg gatcttcacc cgcatcggcg ccgccgacag cctggcgcgt
    13681 gggcagagca ccttcatggt ggagatgcgg gaaatggcga acatcctgaa ccatgccacc
    13741 cggcggagcc tcgtcatcct cgacgaggtg ggacggggca ccagcacctt cgacggtctc
    13801 tccatcgcct gggccatggc ggaatatctg catgacgagc ggcatatcgg cgcccggacc
    13861 ctctttgcca cccattacca tcagttgacg gaactggcgc tgatccatga gggggtcaag
    13921 aattacaacg tcgccgtccg ggagtggggg gaccgggtaa tctttctccg caagatcatg
    13981 gcggggggaa caagccgcag ctacggcatt caggtggcca gactggccgg cgttcccgag
    14041 gaggttgtaa gacgttccgg agagatcctg aaaaacctgg aaaggggaga actggatgcc
    14101 gcggggtcgc ctcgaatcgg cgggggaaag aaaggcggcg acaaaagcag ggcggcccaa
    14161 cagttgagcc tttttcccga tgagcgggag gagatcatca ccggccttaa ggagttggac
    14221 acgacgcgac tgacgcctct ggcggccctg aacctgcttc aggaatggaa agaccggctg
    14281 ataagggggt ccgtttgaag ggacgggagc gcatgaaacg gaataaatgg gtggtctttt
    14341 cccggaccgc cctggtcttg gccatgtccc tgctgtttcc tttctccgcc gccgccgggg
    14401 agtgggattc cctggagaag aaattgagtg cggacggttt tgacgggaaa gagatggcgg
    14461 cccttttcca acgggcggac ctccggttcg atccggagcc catgaccaca aaaattaccg
    14521 aactcatggt ccgggacaac cggcaggatt ccggacaaaa gaagtcaaac cggacggcgg
    14581 tttacggagg gtatctccgt ccggaaaccc tgcggtcggc ccacgcctat ctggatcggc
    14641 accgggtgct cctggggcgg atcagccgga aatactgcgt gcccagggag gtcgtggtcg
    14701 ccatcgccct cgtggaaacc aatctggggg ccaataccgg cagaaggagg gccttcgatg
    14761 tcctggccag catggctctg gctgacgatc tcgaaaaagt gaggcccttt ctgccgccgg
    14821 gggtggtcca tgggggcaac gaggagcagg cccggcggcg gtgccgggaa aaggcggctt
    14881 gggcgtatcg ggaattgaaa tatctgttga aatattcccg ggccaccggg gtggatcccc
    14941 tggccgtccc gggatcgatt tacggggcca tcggtctgtg ccagttcatg ccttccaatg
    15001 tctccctcta cggcatcgac ggggacggca gcggcagcat cgatctcttt accaccccgg
    15061 acgccctgca cagcatcggc aactacctcc accgcaacgg ctggacctgc cgcatggccg
    15121 gaccgcagcg gtggcgcgtc gtcatgacct ataaccacag ccaagtttat gccaatacgg
    15181 tgctggcggt tgcggaccgc ctgcgggcca tggaccacaa gaaaaaaaga aaaaactgac
    15241 tctggtaggg agaatgctac ttgatacaga cccggcggac ctgcttgaaa tccgttacgc
    15301 cctgcaaggc tttctggatg ccgtcctgaa ggagggtggt catcccttct ttcattgaca
    15361 tctcccggag cacctcaaca ggctcgtgct tctggatcat ccgcttgatg ttgtcggagg
    15421 ccacgagaag ttcgtggatg cccatccggc ccttgtatcc cgatttgtca cagatatcgc
    15481 aacctttggg gcggtagagg gtgaagtcgt ccgtatattt cacattgagc ctctcgaagt
    15541 cctcctcgcc gtaactctcg atgagctcct cgaattcctt gggagaggga tgataggctt
    15601 ccctgcagtt cttgcagagg gtccgaacga gacgctgggc gagaatgccc aggagggcgt
    15661 cggcgaaatt cagggggtcg atgcccatgt cgaggagccg ggtgatggtt tccggggcac
    15721 tgttggtgtg gagggtgctg aagacgagat gcccggtcag ggacgcctcg acccccgtct
    15781 tggctgtttc gaaatcccgc atttccccca ccatgatgac gtccggatcg gcccggagga
    15841 aggcccgcat ggcggcggcg aaatcgaaac cgatcttggg ctggacctgc acctggcgaa
    15901 ggccgtactg ggtgatttcc actggatcct cggcggtcca gatctttcgg tccggggtgt
    15961 tgatgtggtg cagggccgca tggagggtgg tggtctttcc cgaacccgtg ggccccacca
    16021 cgaggatcat gccgtagggc ttttcgcaga tgtcgatgag ccccttgtag ttccgtggca
    16081 gcatccccat cgcttccaga ggcagagttt ctcccttggc caggatgcgc atcacgacgt
    16141 cctcaacgcc gccctgcgtg ggaatcgtgg ccacccgcaa ctcgatctcg tcccccccgg
    16201 ggcgtttgaa cttgatcttg ccgtcctggg ggatgcgctt gatggtgatg tccagattgg
    16261 acatgatctt gattctcgaa acgagggcgg cccggtagct gtaaggaacg gtctgataga
    16321 gcccgcagtc cccatcgacc cggaagcgca cctccacgtt cttcttgccg acgttgggtt
    16381 cgatgtgaat gtccgaggtg cggcggctat aggcgtcgtt gataatcttg ttgacgagct
    16441 gcatgatggc gctgtcggat tcggtgacca cttcgccttc ttcttccggc tcggcgtcgc
    16501 cggcatcggc gtccagcttg cctaggatgt cggatatgga ggtttcgtcc tcgggtgatt
    16561 gatagaagta gttaatgaat ttgacgatat cgtcgctcaa ggccacgtcg taacgaatgg
    16621 tcttggtctt catgaggttt tcgatcatat cccgcttcag gatgttgttc ggatcatcca
    16681 cgaggatatt gatgttgccg tcttcaagcc tgttgatggg gacccacagc tcccggcgca
    16741 ggtagtcctt cttcaggttt ttgatgaggt ctccggggat ggggatctta tcgttgaact
    16801 ggacaaactt gcaccggtag aactcggcga gggatttacc aatgttttcc ttggagatct
    16861 tgtatttctt catcaggaag ttttccatcg tctccttttg ttcccgcgat tcctcccagg
    16921 cgttttccag atcctcttcc ttgaggagat cccggctgat gagataatcg aaacgggtct
    16981 tgcgccgccg ggcgaagcgt tcctggttga agaaggctac gcccaatacc tccgttattt
    17041 cgttgagaaa acccttttcg tcttcggaga accgtccgcc gttgacggtg gacttcttgt
    17101 tgaggatctg gaccgccccc atgagctttc cgttgtggaa tatcggtgcg gtaaggatct
    17161 gccgggtgcg gaaacccgat ttcttgtccc aactggagtc gaaggaaagc tccttgtcga
    17221 tggctcggag ttccttttcg tcgtaagcgt cggcgatatt gacgatcttg ccgttattgg
    17281 ccacatagcc ggcgatgctc cggttgttga tgggcacccg tatctcgctc agcttcgacc
    17341 cggaaaggaa catggagaag atctcgttcc gtatgcggtc aacgatgtag atggtgatcg
    17401 agtgggcgtc gaagagattg aggatgccat ccttgagatc caccagaatc tgcttgatat
    17461 tctgggcgga gtggattcta ttggtgatat cctgaagctt cttccgacgt tggagttgtt
    17521 cttccaactc gtttatttgc ttggtagcga gatccgccat ttccatccaa cctcaaaaat
    17581 ccgtgaccag cttggaaaaa tcggcgactc tccggaacag tgccgcgatc tcttcaagca
    17641 gcgacaaacg attgaacctg atatcctctt ccttggccat gaccatgacg gcgtcgaaga
    17701 aggcatccac cggggcccga agccgggcca tttccgtcag ggccccttcg tagcggcatg
    17761 catcgagatg gtccgcggcc cgcttccgca cttcctggaa gacccggtag agttcacttt
    17821 cggcgtcctc ccggaagagt tccggccgca cctcgccgtt tcgaaaatcc ttgaggatgt
    17881 tcgccacgcg cttgcaggcc accgccaggg gctggaagtc ggcgtgattc ttgaacgcct
    17941 ccatggcttc gatctttttc agagcctccg caagatcgga ggcgtcgata gccagcacgg
    18001 catcgacgac gtcgtaggca tgaccctggg cgataagttg gttttcgaac cgcgtccgga
    18061 aaaagtccag gacgtcacgg gcaatctctt cggcgggacg cttcagtttg tcctggagga
    18121 tgttgatgcc cgccgccacc agttccgcca ggcgcagcgg ataacccttg gcgaggatga
    18181 tgttgatgat ccccagggcc tggcgccgca gggcgtatgg atcggcggtt cccgaaggaa
    18241 tgagatttac gccgaagaag ccgatgatgg tgtccatctt gtcggcgatg ccaacgatgg
    18301 cgccgatgtc cgactccggc agttcccccc ccgcggcgag gggcagataa tgctcataga
    18361 tcgccttggc gatcaggggg tcttcaccgg ctagcagtgc atattcacgc cccatgaccc
    18421 cctggagttc cgaaaattcc cccaccatct gggtatccag atccgccttg gccaggacgg
    18481 cggcgcggtc caccggcgcg gcgagggcgg gatcgatctt ggcggtgatg agggcggcga
    18541 gtttgcggaa ccggaggacc ttttcataag atgtacccag gagggaatga tagacgactt
    18601 tacggagctc ctcgaaacgg cggtccaggg agatcttctg gtcttcctcg aagaagaacc
    18661 gggcatcgga aagcctggcc cggatgacct tttcgttgcc cctggtcacc acggacggat
    18721 cccgggccgg ggtgttgttg atggtgatga aatgaggcag cagcctgccc gcggcgtcca
    18781 ccacgggaaa gtacttctga tgggacatca tggtggtggt caaaacctcc cgggggagct
    18841 tgaggtagtc gggatcgaaa acgccgcaga ccgccgtcgg atattcgacg agaaaggcga
    18901 cggtttccag gagatcgtcg gtgatgagag gctggccgtt cacggcggcg gcgacccgct
    18961 gcgcctcttc ccgaatgatt tttttccgtt cttccggatc gatgatcacc caggcttccc
    19021 gggccttgtc cagataatct cgaaagtccg agacggcaaa gggaccgggg ctcatgaagc
    19081 ggtgtccccg agaggcgttg ccggcctcaa tgttttcgat ccggacgggg ataacctcgc
    19141 caccgtaaag ggccaggagc caatgaatcg gccgggcgaa acggagctcc agattggacc
    19201 agcgcatggc ctttttgaag gggatggtca tgatgaattt ggcgagcagg ccgggcagca
    19261 tggcggcgac atcgccgccg gcgatccttt tccgggcgca gaggtactcg cccttatccg
    19321 tggcgatgat ttcgatttct cccaagtcga tgccctgtcc ccgggcgaaa cccaccgccg
    19381 ccttggtagg atttcctccc tcgtcgaagg cggccttgcg ggtcgggccg atcttttcga
    19441 ttacctgatc ttcctgagta accgccacat cggcgacatg gagcaagagg cgccgcggcg
    19501 tcgccatggt ggtgaccggt ccgaatttaa cccgttcccc gttcaatgcc ttgcggatca
    19561 actcgtccat gtcggccatg gcccggggca aaaaggcggc cggaatctct tccgtgccga
    19621 tttcaagcag caattctttt cccattccct gatactcctg taaacccctt tccggattat
    19681 tttatgaggg gataacccat cttttctctt tgtttgatga acagctcggc gctgagccgg
    19741 gccaggttcc ggacccgccc gatataactg gtgcgttcgg cgacgctgat ggccccccgg
    19801 gcgttgagca tgttgaaggt gtgcgagcac ttgaggcagc agtcataggt gggcaggacg
    19861 aggtccattt ccgccacccg aatcccttct gcctcgtaca tgtcgaatag ctttcgcagc
    19921 gtgtccacgt cggccttttc aaagttgtac acggaccatt ccacctcgcc ctgatggtgg
    19981 acgtccccat aggtgacccg gtcgttccac ttcagatcgt aaacgttgtc gattccctgg
    20041 atgtacatgg caatccgttc gataccatag gtgatctccg cgcagacggg acgcaggtcc
    20101 atgccgccca cctgctgaaa ataggtgaac tgggtgatct ccatgccgtc cagccatacc
    20161 tcccagccca ggccccaggc ccccaccgtg ggtgactccc agtcgtcctc gacgaaacgg
    20221 atgtcgtgct cccggggatc gatgccgaag ctcttcagcg aatcgaggta gagttcctga
    20281 atgttcagcg gcgagggctt catgatcacc tggtactggt aatagtgctg caggcggttg
    20341 gggttttcac cgtaacgacc gtcggtcggt cgacgcgacg gttccacata ggctacattc
    20401 cagggctccg gccccagggc ccggaggaag gtggctggat tgaaggttcc cgcccccacc
    20461 tcgaggtcgt aaggctgctg gatgacgcag ccctggtcgg cccagtatct ttccaaagaa
    20521 aaaatcaact cctgaaaggt cacaataacc tccttgttcc ttacgaatta tgtaaggcca
    20581 aatggataac atggcaaaca atcagagtca acaggtattg atgttcatgg cgcccaactg
    20641 ccggagttgc cggaggacct gccacgattt cagctctcgg cccagaagat gttggatgaa
    20701 tgagaccagg aggcgccggg actcgtcgtc ggcctgtggg gagaggagca ggcgggacag
    20761 atttcgaacc ggcagtgtcc tgccgtggag gaggctacgg atcgttccca gagacaacgc
    20821 cggcacagcg ccgggaacgg gttcacagcg gctgcactgc aaaccgcccc gggtgttgct
    20881 gaagatgaag gcccggcccg cctccaggga ggcatgacac cgggtgcagc gatccaggac
    20941 gggctcgtag ccggccaaac cgagcaaccg gatctcgaaa aaggaaagga gactggttcg
    21001 gaaggggccg ttttccatca gggccagaaa atccacgagc aattggaaga ccgcctcgtt
    21061 ttttttcccc tccacggtga aggcgccggt gagttccacc aggtaggagg ccgccacgca
    21121 cttttccaga tcgctacgaa tcacggggaa atgatctatg atttcacatt gatccaccat
    21181 ggcgagggtg tcgccgctcc cccggcggga gaagatgacc tgagtgaagg aaaaaggctc
    21241 cagggcgttg gcgaaccgcc gccgggaccg tcgcgccccc ttggcgattc ccttcagctt
    21301 cccgtattcc cgggtgtaaa aggtggcgat ccggtccgat tccccgtagt cgagggtgtg
    21361 aaggacgatc gccctggtct tatggctggt ccgggcgttc atggtctcga agaaggtcgg
    21421 agtgggttac cgcgccgccc ttcatggtca ggacagcctt tccggtcaga tgccagccca
    21481 ggaagggggt gttcctgctc cgggagcgaa tgccttcggg gcttaccgtc cagcggcgtt
    21541 ccaggtcgat cacggtcaaa tcggcgggtg cgccgggggc aaggctgccg ccggggacgc
    21601 cgaggatcgc caccggtcgg gcactcatcc tggtcacgag ttccggcagg gaaaggaatc
    21661 cgtcggtgac gagccggagg ctcagggcca gggaggtctc cagaccgatc atgccgctgg
    21721 ctgcgtactc gaactccacc tccttgtccg tcacggcatg gggggcgtga tcgctggcaa
    21781 tgacatcgat ggttccgtcc cggagccctt cccggacggc ctcgatgtcc cgttgttccc
    21841 ggagcggcgg atttaccttg gcgttgacgt cgtaaccgcg caggcagtcc gcggtaaggg
    21901 tgaaataatg gggtgccgtt tcggcggtta cctttacgcc ccgggacttg gcatccctga
    21961 tgaggcggac ggagccggcg gaggaaacat gacagatgtg cagcaccgcc ccagtgtatt
    22021 ccgccagcag gaggtcccgg gcgaccatga cctcctcggc gattccgggt atgcccggca
    22081 ggcccagttc cgtggcggcc agcccttcgt gcatggcccc gccggcggag agggagagat
    22141 cctcgcagtg ggacaccacg ggcattccga gggaatgggc gtattccagc gcccggcgca
    22201 tgatggcgcc gtccatgacg ggtttgccgt catcggaaag ggcgacggcc cccgcctcct
    22261 tgagatccca gaactccgcc agggtattcc cctgggaacc aagcgtaatg gcggcgatgg
    22321 gatagacatg gacgaggtcc gcctccccgg ccttgcggag gatgaattcc gttacggagc
    22381 gattgtcgtt gacgggattg gtattgggca tacaggccac ggcggtgaag cccccggcgg
    22441 cggcggccgc gcttcccgtg gcgatggttt ccttgtattc atagcccggt tcccggaaat
    22501 ggacatgcat atcgacgaga ccgggggtga tgacgaggcc ccccagctcc atgatccgga
    22561 ggcctttccc tcgcttcttg cccagatcgc gcccgatccc ggcaatgcgg ccgccctcga
    22621 cgatgatatc cagccggtcg tccaggcccc ggccgggatc gaccaccctt gccccgcgga
    22681 gcaaccagtt ttgtcccgct actgctgatt tcatctatgg actctcacct gacgattata
    22741 tttcccttct actttactca taacggtcta aaacaacgat tatcgtcccg ccgcggccgt
    22801 cggtctcgcc gccggtcggc acaacctctt gctcggcttc atccgtttac cccttgcctc
    22861 ccgtgagcag atacagcagg gccatccgta ccgccacccc gttggtcacc tggttgagga
    22921 taacggagaa cgggccatca gcgatttccg gggcgatttc caccccccgg ttgatcggtc
    22981 ccggatgcat gaccaggacg tcctgtttgg ccagggcgat gttctgtctg ttcaggcaga
    23041 aatgccgggc gtactcccgg agcgagggga agatgttctg atgctgccgt tcgatctgga
    23101 tccggagcat catgacgaca tccgccccgg ccagggcctt ttccagcgaa gaatgcacct
    23161 cgacccccat atgttccatg tctctgggca gcatcgtggc cggaccggca acggcgacct
    23221 gggcccccat ggtcttcaat ccgtagatgt tggaccgggc cacgcggctg tgggcgatgt
    23281 cgccgacgat ggctatccgg aggttttcga tgcgcccctt tttggcccgg atcgtcatca
    23341 tgtccaggag ggcctgagtg ggatgttcat gggcgccgtc gccggcgttg atgaccgact
    23401 gtttaaccag ccccgccagc aggtgggggg ccccggcggc gctgtggcgg atgacgatga
    23461 catcgggatt catggcctcg aggtttcttg ccgtatcgat cagggtttcc cccttgacga
    23521 cgctgctggt cgtggaagag atattgatcg tatcggcgct gagccgtttc cccgcgatct
    23581 cgaaagaggt tctcgtccgg gtgctcgcct cgtagaagag gttgacgatg gtcttgccgc
    23641 ggagggtggg aacctttttg atctcccgtg tggacacctc gatgaaggaa tcggccgtat
    23701 cgaggaccag gatgatctcc tcccgggaga gatccctgat gcccagcatg tcttttcttt
    23761 cccacttcat ctgctattct tccgtctccg gctcgatcac cacttcgtcg gcgccgttct
    23821 tttccgtgag attgacactg acgtcctccc agagggaaga ggggaggttc ttgccgacga
    23881 aatcggcgcg gatcggcaat tcccgatgcc cccggtcgat gaggacggcg agttgaatca
    23941 ggcgcgggcg gccgaaatcg atgagggcgt ccatagcggc ccggatggtc cggccggtga
    24001 agagcacgtc atccaccaag acgactttca tgttgtccag ggagaaggcg atctccgtct
    24061 tgccgatccg gggcttcttg ttgatccgcg acagatcgtc ccggtacagg gtgatgtcga
    24121 ggattcccaa ggggacttcg atgccctcga tggcggcgat ctttttccgc agccggtagg
    24181 ccagatagac cccgccggta cggatgccga tgagcaccag atcgttcaga cccttgttct
    24241 tctccaggat ctcgtaggcg atgcgggtca gggagcggtc gatgccttcg gcgtccataa
    24301 cgatgcgttt tttgtttttc atggtctcct cagtcaaaaa acaaaaaggc ctccccgtaa
    24361 tacctgggga ggcccgttca tgactattct cctcgtcgcc cttttcacac tttccctttt
    24421 tcaatctctc cggattaatt taaaaggcca tatattctca ggcgaagcta gctataaaag
    24481 atcgcccttt gtttgtcaag cccaaagaat cagcgggcag ctccagagaa tcatgaggca
    24541 tgagaactag ttgtggaaat gacatgtgtt aaaggatgga tagaaacacc acatggggac
    24601 tttttgccat gtcgataaaa atgcctttcc gatcgaggtg ggactgtgtt atatactgcc
    24661 ccgcctggag gtgggagtta tggcggtcaa tgctgagcct caggtttttt tgtttttgag
    24721 cgaaatcctg gggcagccat taatcgggcc ggaaggccgt ccggtgggta aaatagtcga
    24781 tctggttgcg actgtgaccg aaccttatcc cgtcgtcacc gaggccatga tcgaccggga
    24841 ggaacaacgg ggagagctgg ttttgtgccg gtttcccgaa gtcgaggagg tgcggctacc
    24901 cattcatgcc gaccggacga ccgccgacga ttaccggccg ccggagttgc gggaaaacga
    24961 actgctcctc aaggactccc tgctggacaa gcagattgtc gacacccacg gcgccaaggt
    25021 gctccgggtc aatgatctcc agtttctcat ggcccgccgg tgtctccatc tggtccatgt
    25081 ggatgtgggc ttccggggcc tcatgcggcg ttccggactg gaaaagttgg tggatctctt
    25141 tctccacacc tttttcgatt ataacctgcc caaccagtac atctcgtgga agtacgtaca
    25201 gcccatttca actcccgatc tcctgcgcct gaagataacc caggatcgcc tggcccggat
    25261 tcacccagcc gatcttgcgg atatcattga agatctcgat gtgaaccagc gcagcgccgt
    25321 attccggtcc ctcgatgtgg aaacggcggc ggagatcctg gaagaaacgg atccgaagat
    25381 ccaggtcagt cttatcaaag atatgaatgc cgctgtggcg tcggatatca tcgaagagat
    25441 gtcgttgagc gaagcggcag acctcctggg cgacctgccc agggataagg ccgaggggat
    25501 cctgaaggaa atggagcagg atatcgccga agacgtcaag gaactcctgg ctcacccgga
    25561 ggaggaggcg ggggggttga tgacctcggc tgtcctccac tttctgccgg agacgacggt
    25621 ggagaaggtg ctggcggcga tccgcggcga agcggaggat atggatttca tctattatgt
    25681 atatgtggtg aatgaggcgg atcgcctcct ggggatggtc agccttcggg aactgttggc
    25741 ggcccctatg gcggcccgtc tggcggatct catggatgaa cgggtgattt ccgttcatct
    25801 cgacgaagac aaagacgaga ttgccgagct gttcgccaaa tacgggctca tggcgatccc
    25861 ggtggtcgat gacttgggta ggatcaaggg cgtgattccc ttcaagaatc tccttgaggt
    25921 cgtggccccg catttgggaa agaaatgatc atggacgaag gcgaaggtca tagatgtcgg
    25981 aacggaatga ggaagggaaa aaagtcaata tctttcgccg tctgttccag aagagattct
    26041 ggcgctccct gggattgctg ttcgccctta tcgggccggg aatcatcacc tccaacgtcg
    26101 acaacgatgc cggcggcata accacctatt ccctggccgg cgctcagtac ggcctctccc
    26161 tcctctggac cctcatcccc atcaccgtgg ccctgatcat cattcaggag atgtgcgccc
    26221 ggatgggggt ggtttccggc aagggactat ccgatctcat ccgggaacgg ttcggagcca
    26281 agatcaccgt ttacctcatg atggtcctct tgctggccaa tctgggcaac accatctccg
    26341 aatttgcggg catcgccgcc agcctggaga tcttcggtgt cagcaagtac ctttccgttc
    26401 ccgttggggc attcttcgtc tggtggctcg tcgtcaaggg gagctacaaa tcggtggaaa
    26461 aggtctttct cgtggcctgc ctgttttatg tttcctatat catttcagga tttatgggaa
    26521 aaccggactg gggagagatc gggatcgctt tggtcacccc gacgctcagc gcagaccccg
    26581 cctttctcac catggccgtc ggcgtggtcg gtacgactat cgccccgtgg atgcagttct
    26641 atctccagtc ttccgtggtg gacaagggcc tgaaggcgga ggactatgcc tatacccggg
    26701 tcgatatcat cgtcggttcc ctgacggtca atatcgtggc ttttttcatc atcatgctct
    26761 gcgccgtcac gctctttcaa caggggatcc ggatcgagac cgccaaggat gcggcccagg
    26821 ccctggcccc cctggccggc gcctactgta cttatctttt tgccttcggc ctcctgaacg
    26881 cctccctgtt tgccgcctcg atcctgccgc tgtccacggc ctacacgatc tgcgaggcct
    26941 tcggatggga gtccagtctt aatcacaagt ttaccgaggc cccgcagttc tatggcctct
    27001 attccctcat gatcgtcctc ggcgccgcca tcatccttct ccccaacatg cccctgatcg
    27061 ggatcatgta ctattcccag gtcatcaacg gcctgctgtt gccttttgtc ctgatcttca
    27121 tgctgctcct cgtcaacgac cgccggatca tgggtgagta tgtaaacggc cggacgatga
    27181 atttaatcac ctggctcacc gtgggcatct tgatccttct ctccctggcc atcgtcatca
    27241 cctccctgct gccggcctga accatcgagg gagggcgaag caaataattc ttgacagcat
    27301 gggcaaactt ctatatcttt tggtgaccga cggtcagtat gtgacttacg ttcagagtgt
    27361 tgcggggttt tcattacaca ataaacgaca ccggagaagc atgcctttcc ccccggcctt
    27421 ttctgaagtg cttccgccgg taacctgatt ggaccagatt cggaaaaggc gaaaaaaagg
    27481 aggcaacgat gggtaacgga ttggtgagtt tgcgggatca acagtttgtc cttttcgaac
    27541 aaatcgggat cgagaatctc tttacgaaag atctgtatgc cgactattcc cgggatgtag
    27601 tgctgatgat gcagggcgag gcggagaaaa tggcggtgaa cgtcatcctg cccacttacg
    27661 ccgaagcaga tcgggagggg tgccatattg acaagaatgg ccaggtgcgg gtcccgaagt
    27721 gctttcttga agtcttcaag aagtattgcg caggcgggtg gctggccccg atgaagtcgc
    27781 cggaggtggg cggtcagaac atgcccgttg cggtctccac gtgcttcacg gagatgtttg
    27841 cttccgccaa cgtcgccttc ctgctctatg ccgggcttac cctgggggcc ggcggcctcg
    27901 tggagaactt cggcacggag gaacagaaga aaaaatatat gtacaaactc tacgccgggg
    27961 agtgggccgg gacgatgtgc cttaccgagc cgggcgcggg gagcgacgta ggcgccctga
    28021 agaccacggc gaagcgcctg cccgacggca agttcctcat cacggggacg aagtgcttca
    28081 tctcctccgg tgatcatgat ctgaccccga acatcatcca tcccgtcctg gcacggatcg
    28141 aaggcgatcc tccggggacg ggcggcatct ccatcttcat cgtccccaag atccgggtca
    28201 acgacgacgg cagcctcggc gagcccaacg acgtgaagac cgggaacgtg gagcacaaaa
    28261 tggggatcaa gggttccgcc acctgcaccc tgaacttcgg cgacgagggc aagtgcatcg
    28321 gcgaacttct gggtcaggaa cgccaaggca tgcggatcat gttccacatg atgaacgagg
    28381 cccgcctgga agtgggcatc cagggcttgg gccacgcctc cgcggccctg gagcacgccg
    28441 tgcagtacgc caaggagcgg atccagagca ccccggtctg ggagatgaag aatcccgacg
    28501 ccaaggcggt gcccatcatc atgcaccccg acgtacgccg ggacctcctg tggatgaagg
    28561 cctatgtgga agggatccgg gccttgaacc tgtttacggc ttattgtatc gacatgtcca
    28621 gggtggggga gacccccgaa gagcgggcca agtatcaggg cttcgtagaa ctcctcaccc
    28681 cggtatgcaa ggcctactca tccgacatgg cctgcctggt cacctccaag gccatcgacg
    28741 tgtacggcgg ctacggctac tgccaggaat atccggtgga gcagtacctg cgggactgca
    28801 agatcgcctg cctgtacgag gggaccaacg gcatccagtc cctggacctc gtgggccgga
    28861 agcttgggat gaacaagggc atgaacgtca tgaacatgat ggtggagatc gacgccacga
    28921 tcaaaaaggc aaagggtagc gaagaattag ccaagtacgc cgccaagctc gaagaggcct
    28981 acaacgccct tgtggatctc accatgacct tcgcggccct ggggaagagc tccagcttcc
    29041 ttatccccat cctctacgcc tcgccgtatc tcgatctttt cggcgatgtc ctccttggcc
    29101 attttctctt ccagggcgcg tccatcgccc gggaaaagct cgacgggatc ttcgcggcca
    29161 agggcgccga cagcatcggg aagaagcggg ccctggtcca taccgatccg gatgtggcct
    29221 tctacgaggg caagatcgcc tcggccaagt tcttcgccct ggaagttctg gcctcggtca
    29281 aggccaaatg tgaagcgatc aaggcggggg acaagacagc catagaaatg gccatcgagt
    29341 ccttcccatc ctgaaagacg tcataggccg aaataagttt ccgggcggca gtttatagac
    29401 gagcactcct taaaggccct ctccggtaat ggttggggag ggccttccct ccccggaggg
    29461 gccgtaccag cctttaaacg ccataaacca ggaagggtct ggtcggctaa cggatcagac
    29521 tgcccaggcg gttccagcgc aacgatcttt cctctttgtc ccaggaccag tagatgacat
    29581 aggccttccc catgacgtcc ttgagatcga cgaagcccca gaaacggctg tcatagctct
    29641 ggtcgcggtt gtcacccatg gtaaagaccg accccttggg aacggtgacg gggccgaaat
    29701 tgtcccgggg ctggatggcg cccggaatga cgaacccgtc cacatagacg ccgtacttgt
    29761 cctcgtaggg catgccgttg agaaagatct ttttgtttct gatctcgatt gtgtcgccac
    29821 cgacgccgat cacccgtttg atgaaatcct tggagcggtc ctcgggatag atgaacacga
    29881 tgatctcgcc ccgtttggga tctcggacgg ggatcagggt cttgcggaaa taggggatct
    29941 tcaccccata gatgaattta ctcaccagga tatgatcacc gatcaacagt gtgggcttca
    30001 tggatccgga ggggatcttg aaggcctgga ccacgaaggt gcggataaaa aaggcgatga
    30061 gaatggcgat gatgatcgcc tcggcgtact cctgaacttt tgatttcggt tttttcgtca
    30121 tgtaggctac aggtccttta tcaagaatat acggcattca gggccaggat caccatgaca
    30181 aaggcgagac agccctttac acagatacca gcgacacgcc ccagcaccat gccgaggctg
    30241 gcccgaaagg ccattttcag gcgcttcctg tgcaccatct ccatcatcaa cagtccggcc
    30301 atgccaccga ggaaaatccc ggcaatcgcc ccgaagccga ggaaaaaagg ggtcagggcc
    30361 gcggccccga tcatgcttcc gatcagtgaa gcccagaatc ccttcatgga aagtccgaac
    30421 tgtgatgcca catgcatgcc gatgatgaat tccagtgttt ccgccaggac cgcgagggca
    30481 acgatgacca ggagcagttt ccagccgatg acggtaaacc ccgtgacggc cgcgtaagca
    30541 agtacgacca ccgcgatgat cacggctccc ggtaggccga agataatgga gaagagcccc
    30601 agcagtaaaa agatcacaaa gagggacaga ccgataattt ccagagtcgg caagttattt
    30661 cttccttctg aacttggagg gcatccattt atcccagacc agaacaaagg gactggcgat
    30721 aaaaacggtg gaataggcac caacgattac cccgacaatc agggcaaagg caaagtcatg
    30781 gattaccggg ccgccgaaga aaaagaggga caggaggact aagaagacgg taaaggcggt
    30841 cagaatcgtc cggctcaggg tttggttgat gctgtcgttg attgtttccg ccaggggttt
    30901 cttgatattc ttccgggcat tttcccgaat ccggtcgaag atgacgacgg tgtcgttgat
    30961 ggaataaccg atgatggtca ggagggcggc aacgatggtt agatcgaatt ccttgttcag
    31021 taaggaaaag gcgccgatgg tgatgatcgt gtcatggatc agggccagga tccccccggc
    31081 ggcataacgg aactcgaaac ggaaaccgac atagatgagg atgccgatcc aggagaaaaa
    31141 gatggccagg agggcctttt tcgtcagatc cttgcccacc ttgggcccca ccatttccac
    31201 ccgccgcatc tcgacggcgt tctgtccgaa ggtattattc agcttttctt ccacttttcc
    31261 cgacaggctc ttgagatccg tagtgccttc ccctacgcgg acgacgatct cctggggacc
    31321 gaactgctgg atgatgctgt tttctatgcc gaggggctgg aaggcctctc ttatcttgtc
    31381 gatggtgaag gggacgttga atttgacctg gatgagggtg cccccggcga agtcgatgcc
    31441 gtaattgagt cccccatgcc aggcaataga ggcaaaggtg atgatgatca tggccatgga
    31501 gatgctgaag gccagtttca ttttaccgac aaagtcgatg ttgatgtccg gtttgataaa
    31561 ttccacgaat gccatgaatg aactccttac acgctgatcg tctggatctt gcgattccag
    31621 atgaaatagt cataaataat ccgggtcacg aagacggcag tgaacatatt ggcgatcagg
    31681 ccgatggtca gggtgacggc aaaccccttc acgggtccgg tgccgaatcc gaagagaaag
    31741 agagcagcga tgagggtggt tagattggag tcaaaaatgg cgagaaaggc cttggtgtag
    31801 ccggcttcaa ccgatgcccg tggcgattta ccgcccctta tctcttcacg aatcctctct
    31861 aaaatcagga tgttggcatc gacggccata cctatgccca ggacgatgcc ggcgatgccg
    31921 ggcagggtca gggtggcccg gaaggccgcc agggtgccga agattatgat gatgttgagg
    31981 atcagggcta aattggccac cagcccggaa atcttgtagt agatcatcat gaacaggatg
    32041 acgagcatca ggccggcgat ggttgaccag atccccttgt caatggagtc ctgtcccagg
    32101 gacggtccga cggttctttc ctccaggatg gtgaccgggg cgggcagggc gccggcccgg
    32161 aggacgatgg cgagatcctt ggcctcgtcc atggtgaagg tgccggtgat ctgggcgttg
    32221 ccgccggaga tccgttcctg gatcaccggc gcggaatgga tgaccccgtc gaggacgatg
    32281 gccaggcgct tcttgacgtt ttccgccgtg atccggtcaa aatcctgggc cccctgggcg
    32341 ttgaacttca gggagacgta gggttccccg aaccggtcgg cgatggacac cttggccgtt
    32401 tccagggagg cgccggtcat gagggtcttg ttcttgacga gataggggac ctgggtccgc
    32461 cgtcccgatt cccggtctaa atgggttccg tacgcgatga tgctcccttc ggggatgttt
    32521 cccctcatag cctcttccag gctgtgttcc tcatctaaga gcttgaactc caggagggcc
    32581 gtcttgccga tgaggttctt ggcccggccc ggatccctga tgccggggag ttggacgatg
    32641 atccggtcct cgccctgggg gatgatctcc ggctcggtga tgccgaattg gtccacgcgg
    32701 ttccggatgg tttccaggcc ctgttccatg gccagcttct tgatgtcctg ggcccgtttc
    32761 gccttgagct ttatctggac gatttccttg ccgtcccgct ggctcgctga cgccggctcg
    32821 aaatccggat agttgtcctt gaggatcttt tcgaaggcct cccgcgccgg atgatcggga
    32881 aattcaacgg ttatcgagtc cttttgcgtc ctggcgatgc cgcggaagcg gatcttcttt
    32941 tccatcatcg tctctttgat gtcgttggcc atccgttcga tggttccctc cagggccttg
    33001 tccgtctgca cctccaggac gagatgcatg ccgccctgaa gatccaggcc gagatgaatc
    33061 ttgtccgccg gcaggttgtt tttccagaat ggcggcaacc ggtccgtcat gacggaggga
    33121 gtgagataaa aaagggcgac gagacaaacc gccaaaacga tgagcgccct tatgccgata
    33181 cttttaatca tgccttacct cgcttggttg ttattctttc cctgcttttt ggagcacggc
    33241 gctgatgaag cttcgcgata ccttgatctt gaccttgtcg gcgatttcga gggtgacgat
    33301 ggcgtcggta agcccgacga ccttgccgtg gattcctccg gaggtaagca ccgtgtcacc
    33361 atgggccagg ttgtcgatca tggtcttcaa ttccttctgt ttttgctgct gcggcctgat
    33421 gaggagaaaa tagaatatgg cgaagaggat gaccatcacc acgatgaaac tccagtctcc
    33481 ccctcctccc atctttcctc ctccggcgct tcccattgcg taggcagtag cgatcacttc
    33541 tcaaaacctc cttttatatc gacgttatca ggttcccaat gattctttta tgaacaataa
    33601 gcttcgcttt gtgaacatta agcttcgctt tccgtaaagt tcccaagaat ctcatatatt
    33661 tcccgggaag cgaccggcgg cgatcgcctc ccggatcctc tcnnnnnnnn nnnnnnnnnn
    33721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    33781 nnnnnnnnnn nnnnnnnnnn nnaatctcat atatttcccg ggaagcgacc ggcggcgatc
    33841 gcctcccgga tcctctccat gagccgcaga taatggcgga ggttgtggat cgtgttcagg
    33901 atcagcccta aaatctcccc gcttacaaac aggtgcctca agtaggcccg ggaatagttc
    33961 ctgcaagtat aacagtcaca tagctcatca atgggcgctt ggtcctcccg gtagcgggcg
    34021 tgctttataa caatctttcc ctcgcttgta aataacaatc cgtggcgggc gctccgggtc
    34081 ggcattacgc agtcgaacat gtcggcgccg cgggcgacgc aggcgacgat atcctctggt
    34141 gtgccgacgc ccatgatata acggggccgt tcttccggaa gttgcggggt ggtttccccc
    34201 accacggcca tcatctcctc cttaggttcc ccgacgctga gcccccccag ggccaagccc
    34261 tcgaagggca tttccagcag tgcttccaga gatcgccggc ggagttccat gtgcatcccg
    34321 ccctggacga tgccgaagag ggcggcggcg tccccccggc gatgatggca gcgccgggcc
    34381 caccggacgg ttcggtccag ggaaagttcc gcctcccgaa atgtggccgg atagggggtg
    34441 cattcgtcga ggcacatcat gatgtcggcg cccagggcaa gctggatgtc cacggccgcc
    34501 tccggggtga ggagatgccg ggagccgtcg atgtgggact gaaacaggac gccctcttcg
    34561 gtgatcttcc gcagcgcccc gaggctgaag acctgatagc cgccgctgtc cgtcagtatc
    34621 ggcccggacc agttcatgaa cttgtggagc cctcccgccg ccgcgacgat tccgtgcccc
    34681 ggccggaggt acaggtgata ggtgttgctc aggattatct gcgcccccag ttcccggacc
    34741 atgtccggcg tcagggcctt caccgctccc tgggtcccca cgggcatgaa ggccggggta
    34801 taaatgactc cgtgcgccgt acgcatcctt gccagacggg cgccgctgtg ttcgtccttt
    34861 tttatcagtt caaagtcgaa attcatttcc ctccatcccg tcagattatc aacatgcagt
    34921 ctccataact gtaaaagcgg tagtcccggc cgatggcctc cgcataggcg gcggccacgg
    34981 cctgcttgcc cccgaaggcg caggtcaaaa gatagaggga cgatcggggg aggtgaaaat
    35041 tcgtcagcag ggcccggacc ctcttgaaac gataaccggg gtagatgtac agagacgtcc
    35101 acccggccgt gggatgcacc gtcccgcctt cgtcggcgac cgtttccaag gcccgggtgg
    35161 ccgtcgtccc cacggcaacg acccgggcgg caccgttgat ggcggcggcg gcttccgcac
    35221 cgatttcgaa gtattccgct tccatccggt gatcctcgat gtccgtcgcg gtgatgggca
    35281 ggaaggtccc gtagccgacg tggagggtaa cggcggcgat cccgatcccc cgtttacgga
    35341 tggactccag cacggcggcg gaaaaatgaa aacctgccgt gggggcggcg acggaaccgg
    35401 gcgcgcgggc gtaaatcgtc tgatagcgat cccggtcccc cgcggaacga tcgtcctccg
    35461 gcgtcctctt gatatacggg ggcagggggg gccggccgtg gcggttcaga tacgcggcga
    35521 agggctcggg ggaaaagaag cggagccgcc atattttctc cgaacgacgc tccttcacct
    35581 cggcccggcc cgcgccgggt aactggataa cggtgccgac ccggaccctt ttggccggtt
    35641 tcagcatggc ttcccagagc tctccttcgc ctgtcgccgg gattttcgac agcaggagga
    35701 tctcgataac ggcgccgctt tccttccgcc cgacgagccg ggccggaatg acccgggaat
    35761 cgttgaccac caaaacgtct ccttcccgca gatattcggg gatgtccctg aagatgcggt
    35821 ggcggacctg ccccgtagcc gcttccagga ccagcatccg ggagcgatcg cggtggggca
    35881 ggggggcctg ggcgatgaac gatggcggca ggtggtaaga gaattcctcc agcttcatgc
    35941 cgcggttccg aaatagacga gtgccagccc cgcgatgatg gcggccagac ccatgaggcg
    36001 cagggtggtg tccggaacct cgctcatctt ggcgaggtag atcttgagtt tttccgggaa
    36061 cgcgaaatag ggcagaccct cgatgatgaa aaccatgccc aggacgcaga ggaaatattt
    36121 cattttcatc tccttttggc gactccctaa cacatcctca tatccttgac aatgaggcgg
    36181 cgccgatttt tacaaaatgc ttgacactac cagccgtttc atatacgtaa atccggattt
    36241 tacaggtcgt caatttacca catcaataag caactacagg aagcaggctg attccttccc
    36301 cataaggatc aacgcaatgg tggtgatttc tgtaaatagg cttggatgaa gtgggagagg
    36361 tgtaatatca tgaacatgga tgcgtaatcc aaaaaatagg aggaggtagg tatggcagaa
    36421 gaagagaaaa aaccaaggtt gacaagggcc gaaagactgg agagagaacg ggagaagaac
    36481 cttcagcact ggctggcgca ggatgagatc ctcttcaagc accgggaaga ggcgatgaac
    36541 atcggcggtg atgctcaggt ggagcgtctg gccaaacaga acaagaaacc aatgcgtcag
    36601 ctcatttccc aactcatcga tccgggtacg gagttcttcg aggtaggtct ggatgccggt
    36661 tacgacatcg gcaagaaacg cctgtacggc ggcgagatct acgaatcgga aaaacgcggt
    36721 cacatccccg gcggcggcat ggttaccggc gtaggcgttg tccacggcaa ggactgcatg
    36781 atctttgcca acgagaaccg ctatgcggcg gggacctatt tccccatcac cctgaagaag
    36841 cacatgcggg cccaggctat tgccgaggcc tcccggctcc cctgcattta catcgccgac
    36901 tccggcggca taaacctgcc cctacaggtc ggctgcttcg ccgacgacgg ccacttcggc
    36961 agcatgttct acaacatgtg ccgcatgtcc gccatgggca tcaagcagtt caccctgtcc
    37021 acgggcggca acacggcggg cggcgcctac attgt
//
LOCUS       DAOSMF010000011        36090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_1562_length_36090_cov_24.035271, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000011 DAOSMF010000000
VERSION     DAOSMF010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 36090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36090
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1562_length_36090_cov_24.035271"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="PLT63_02725"
     CDS             <1..215
                     /locus_tag="PLT63_02725"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOD97665.1"
                     /translation="VIRAACKPIPSLGREQTTIDRDGNPVLLAVRGRHDVAVIPRILP
                     VCEAMVCLVLADHLLRQEALGITRRG"
     gene            232..1044
                     /locus_tag="PLT63_02730"
     CDS             232..1044
                     /locus_tag="PLT63_02730"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HOD97666.1"
                     /translation="MKTGIFPLGIIGGTGGMGKWFADFFRRNGYPVVATGRTRGPDFA
                     SLAATCPVVIVSVPIAITEAVIEKIGPCLPPESLLMDLTSLKEGPVRAMLAATAAEVI
                     GLHPLFGPGVTSLSGQNIAVCPARGNSWLPWLRDILEKNGAHLVETTPARHDELMAVI
                     QALNHFLTAALGLSVAAAGIEPEELRRFSTPVFRERLDHLHRLLSHPDLYAHLIAGNS
                     YGKKSIISTINHLEALLAPVRGGDTAALAAQLRAISASSPSPRSDTHESAEE"
     gene            1085..1294
                     /locus_tag="PLT63_02735"
     CDS             1085..1294
                     /locus_tag="PLT63_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97667.1"
                     /translation="MDKWHTPDDLVVSSAILAKEYARLSSTTLRRDLTELDRLDLIVR
                     DKDKYKGNIEIMRGYMPQRKARPVR"
     gene            1585..1848
                     /locus_tag="PLT63_02740"
     CDS             1585..1848
                     /locus_tag="PLT63_02740"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HOD97668.1"
                     /translation="MKWRLVFTEQAQKDAKKIAPSGLRPQVSKLLEILQNDPYQKPPP
                     YEKLVGDLTGAFSRRTNIQHRLIYQVLVDIKTVKIIRMWTHYE"
     gene            2257..2871
                     /locus_tag="PLT63_02745"
     CDS             2257..2871
                     /locus_tag="PLT63_02745"
                     /inference="COORDINATES: protein motif:HMM:NF020996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HOD97669.1"
                     /translation="MNPDAIREKFGDDYIADERTFMMGIDQRFTAHMAERFRGRRVLE
                     TCTGAGFTTIALARAASQVITVEIDPYHQTQAKKNLQRAGLLSSVEFVSGSILDPSLL
                     AGLPPFDAAFLDPDWAITGPDHVYRFVHSNTQPPADILLKTILERTRNVAIVLPPLLD
                     TRELAGLPRNECQKLYLGESHELYCLYFGDLAAGFGDTAFRVSV"
     gene            2944..3249
                     /locus_tag="PLT63_02750"
     CDS             2944..3249
                     /locus_tag="PLT63_02750"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOD97670.1"
                     /translation="MEYSIRPISNEDRESIMDIFNHYVENSFAPYPENKLPYQAFDMF
                     RQMSHGFPTGSIKDQNGKIVGFGMLRAHNPMPTFSQTAETTYFIHPEHTGKGRNKDE"
     gene            3242..3988
                     /locus_tag="PLT63_02755"
     CDS             3242..3988
                     /locus_tag="PLT63_02755"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOD97671.1"
                     /translation="MNERHSKWQTKEIAETFVEGVRGAIPGAKLQLEVISKIITAWFP
                     QPSRILDLGCGDGIIGRLLLEEYPEARVIFADFSEPMLEKLRIKIGINQRAKVINADF
                     ATSDWVKGSELEKPFDIVVSGFAIHHQPDERKVELYTEIFCLLRSGGIFLNLDQVKSK
                     NPSTSEIFDGFFLDHIRRALSISDQNEMMNQIEKAYYEDKKENIPAPVEKQCQWLSDI
                     GFEDVDCFFKTFELALFGGKKTFTRVAGGS"
     gene            4065..4208
                     /locus_tag="PLT63_02760"
     CDS             4065..4208
                     /locus_tag="PLT63_02760"
                     /inference="COORDINATES: protein motif:HMM:NF025189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HOD97672.1"
                     /translation="MSLIKSCKDCNVNPWEYFDDLLQNIMSHPSNRLRELLPDQWKPQ
                     TKE"
     gene            complement(5136..8516)
                     /locus_tag="PLT63_02765"
     CDS             complement(5136..8516)
                     /locus_tag="PLT63_02765"
                     /inference="COORDINATES: protein motif:HMM:NF016358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Swt1 family HEPN domain-containing protein"
                     /protein_id="HOD97673.1"
                     /translation="MAITNHERVGKALSLLKEGLRPFVERELKSQYQQSWFDELKASL
                     PPQQLSFAGTEAEPFGDIAVVLAALWNQWNSVFKKTLGHTERSLVSELRDHRNKWAHQ
                     EPFSGDDADRALDSAERLLAAVSAPQAEDVRKLKMELRRTIYDEQVRSEKRKSAGSAI
                     ESAAAGNLKPWREVVTPHKDVASGRYQQAEFAADLWQVRLGEGTPEYRDPIEFFRRTY
                     LTESLKRMLVGAVRRLSGQSADPVVQLQTNFGGGKTHAMLALYHLLSGIAPTELAGID
                     AVMQEAGTATLPSVRRVVLVGNRISPGNPVRKQDGTVVRTLWGELAWQLGYAAGGESE
                     AGKAFDQLAADDERATSPGDVLRELFVRYGPCLILIDEWVAYARQLHDQSDLPGGSFE
                     TQFTFAQALTESAKLAGNCLLVISLPASDTSGSPHAQTDDAEVGGQRGREALDRLRNV
                     VGRLESSWRPASAEEGFEIVRRRLFEPLADPAQFKDRDVVARAFADFYRTQQAEFPPE
                     CRDADYEKRIRAAYPIHPEIFDRLYTDWSTLVKFQRTRGVLRLMASVIHSLWEKGDRN
                     PLIMPANISIDDSRVQSELTRYLSDNWPPIIERDVDGPNSLPLKIDGEVPNLGKFAAC
                     RRVARAIYLGSAPLTKAAHRGLEDRQVKLGCVMPGESPNVFGDALRRLAAAATYLYQD
                     GPRYWYSTQPTVTKLAEDRAEQLKRDPDKVMQELDGRLRKDLERKGDFSRIHPLPQSG
                     QDVSDDLDARLVVLGINHPYSKEPGSAAEAAAKAILESRGSSPRHYRNTLVFLAADKT
                     RLQDLDEAARKFLAWESILTEKGEKGLNLDPQQVKQAETQRTAADSAVTARLPETYQW
                     LLVPVQSTPQSAVEWQALRLSGQDALAVRASKKLKNDELLITTFAATRLRMELDRIPL
                     WRGNHVAIKQLAEDFASFSYLPRLKDSSVLVGAIRTGLPLMLWMQDSFAFADSYDESA
                     NRYRGLRCGVEVYIAGGETQGLLVKPDVACRQMDEEKAKTTVGGVTVGPTAGGEGTTT
                     IIPVGGPMGTPDTPAVAPKLKRFHGTVILDAARVGRDASRIADEVIAHLSGLVGATVT
                     VTLEVEADIPSGAPDHVVRTVTENSRTLKFASQEFEKE"
     gene            complement(8521..9111)
                     /locus_tag="PLT63_02770"
     CDS             complement(8521..9111)
                     /locus_tag="PLT63_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4276 family protein"
                     /protein_id="HOD97674.1"
                     /translation="MKKIVFLLEEPSMKAFIEEFLPRLIPDLEFLCITHEGKQDLEKS
                     IPRKLKAFRRELFVIIRDNDGADCRAIKARLIDVCKNEGKSDVLIRIACQELESWYLG
                     AMDILAAVYARPKLTGLNLKSKYRNPDRLGNPSSEVIKLIPEFRKIEGARRMGMAMPL
                     EKSDNYSRSFQVFIEGIQRMLKSTTGKKVRLRRRQE"
     gene            complement(9114..10307)
                     /locus_tag="PLT63_02775"
     CDS             complement(9114..10307)
                     /locus_tag="PLT63_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOD97675.1"
                     /translation="MDRMEIQSMQIESIDILNYRLFKEASLMHLKRLAVLVGENGAGK
                     STFFDVFSFLKDALAQNVAKAVAKRGGFKELVSRGTEGPISIVLKFRETGKRLATYEL
                     SVAFEGGKVVVDREVLKFRRGQRGQPWHFVDFSRGKGTAITNEAVYGEPGAKKERKEY
                     ILDEPTVLAIKGLGQFKEFRVVSEFRNLIENWHITDFHIADARPSAEEGYAEHLSTRG
                     DNVAQVAQFLFDHHQDRFNDILDAMKRRIPGVEKVEAKPTEDGRLVLRFQDGAFKDPF
                     IARYVSDGTIKMFAYLVLLHDPNPFPLLAVEEPENQLYPELLQELAEEFRDYARRGGQ
                     VFISTHSPEFMNGLNLDEIYWLTKKEGYATIHRGADSEILKSLVAEGDYPGTLWRQGV
                     FKGAW"
     gene            complement(10339..13242)
                     /locus_tag="PLT63_02780"
     CDS             complement(10339..13242)
                     /locus_tag="PLT63_02780"
                     /inference="COORDINATES: protein motif:HMM:NF018357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1156 domain-containing protein"
                     /protein_id="HOD97676.1"
                     /translation="MNYKKKLIEVALPLDAINKASAREKSIRHGHPSTLHLWWARRPL
                     AAARAVIFSQMVDDPSAHPDLFPTEKAQEKERKRLFKIIEDLVLWENTTNEKVLQQAR
                     DEIWQSWRYTCAENAGHPRAKELFDRHKLPAFHDPFAGGGALPLEAQRLGLEAYASDL
                     NPVAVLINKAMIEIPPKFAGKPPVNPEWQQLPDGQKALRTWKGAQGLAEDVRYYGKWM
                     RDEAEKRIGHLYPKVEITTEMVKERSDLKPYLGKKLTVIAWLWARTVKSPNPAFANVD
                     VPLASTFMLSAKAGKEAYVEPVIENGGYRFTVKVGKPKDAEAAKNGTKLSRGAHFQCI
                     MSGTPIAADYIKAEGKAGRMGVQMMAIVAAGDRGRIYLPPTAAMESVARQTNPAWQPE
                     GTLVEDARAFTPTLYGLAKWYDLFTARQLVTLTVLSNLVQEACAMIKRDALAAGLLDD
                     SKFLDPGIGIISYADAVGVYLSFALSKQADLGNSLCRWEPIAQCPRQLFGRQAIPMVW
                     DFAEGNPLGDSSGAWIVLIDGITKAFAKAFEFVSSKVIGSALQADASRQDASKAKVVS
                     TDPPYYDNVPYADLSDFFYVWLRRSLQPVFPDLFATLAVPKTEELVAFGYRHGGKVDA
                     EAFFLNGMKQAMQRLAEQSHPAFPVTIYYAFKQVENDGADGNASTGWETFLEAVICAG
                     FSTTGTWPMRTENDSRLRGQASNALASSIVLVCRKRVADAPISTRREFINVLKAELPV
                     ALAHLQRSNIAPVDLAQAAIGPGMAIFTRYAKVLDAEGKPLTVREALALINQTLDEVL
                     AEQEGDFDADSRWALAWFEQAGFDEGEYGVAEVLSKAKATSIDGMKRDGILASTGGRV
                     RLLRPDELSAAWDPEKDKRIPAWEMVHQLVRALETGGEGAAAVLVTKLGSKAEVAREL
                     AYRLYTLCERKKRAAEALSYNSLVQSWPEIMRLATGSEKSRGVQADIFDNDEI"
     gene            complement(13389..13622)
                     /locus_tag="PLT63_02785"
     CDS             complement(13389..13622)
                     /locus_tag="PLT63_02785"
                     /inference="COORDINATES: protein motif:HMM:NF027067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiT-type zinc finger protein"
                     /protein_id="HOD97677.1"
                     /translation="MKTKNCPICGAGKLKKKVETESFEYKGQTKVIPNYITYACSECG
                     EAIVDSTTLKESGKMLEDFKLKMEVAFDDEIFR"
     gene            complement(13629..14792)
                     /locus_tag="PLT63_02790"
     CDS             complement(13629..14792)
                     /locus_tag="PLT63_02790"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD97678.1"
                     /translation="MPAVVVTGARQTGKSTLVKVLTLEKRSYFSLDDMDVFELARQNP
                     EALVGGAAKVTLDEVQREPDLLLAVKRAIDSDRQPGRFLLTGSANLLLMQGVSESLAG
                     RASYLTLWPMTRREQLGLGRCGLWEELVAANDAEWPDLLSVDTSGPEDWLAFARRGGF
                     PTPAVHMTRAEDRMVWFDGYVRTYLERDLQVLSSIAALPDFRRLMRAACFRLGQLVNQ
                     TEMARDLSLKQPTIHRYLNLLETSYLLIRLPAYSVNRTKRLLKSPKLYWGDVGLAMHL
                     SGMAEPTGAYLENIVLLDLLVWQCGRLQNTEILYWRTTTGEEVDLVIETDGKVLPIEI
                     KSTNRPRIKDAMNLLAFQNEYGADARAGLLLHTGTVIEWLTPRVLAVPWWKVI"
     gene            complement(14892..18473)
                     /locus_tag="PLT63_02795"
     CDS             complement(14892..18473)
                     /locus_tag="PLT63_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOD97679.1"
                     /translation="MIRLEDLQPNAAVCGLLPDCLITVVSVQWFGSEALELTYKTPTG
                     KVANELLYRYDEPRLDLVEQGRPWSFDGDGALFRLVSEAHRIRLAHLFDPVLAVHTSI
                     VDPLPHQITAVYEAMLPRQPLRFLLADDPGAGKTIMAGLLIKELVARGDLQRCLIVCP
                     GSLAEQWQDELYHRFHLPFEILTNDKLEAARTGNWFLETNLVIARLDKLSRNEDVQQK
                     LQTPDCRWDLVVCDEAHKMSATIFGTETKYTKRYRLGQLLSTLTRHFLLMTATPHNGK
                     EADFQLFMALLDGDRFEGRFRDGVHVADVSDLMRRMVKESLLKFDGTPLFPERIAYTV
                     PYKLSEAEAKLYKAVTEYVREEFNRAEALQNDKRTGTVGFALTILQRRLASSPEAIYQ
                     SLRRRRERLESRLREMEVLQRGGQAAPILAADLLALDAEDVEDLEDAPDNEVKAAEEE
                     ILDQATAARTITELKAEIETLKGLESLALGVRRSGTDTKWRELSTLLGEIFTTAAISN
                     RLAEPITPFGAGAIPRHTPSLQQKLVLFTEHRDTLNYLENRITTLLGRKEAVVMIHGG
                     MGREERMKEQESFKHDPKVQVLLATDAAGEGINLQRAHLMVNYDLPWNPNRIEQRFGR
                     IHRIGQTEVCHLWNLVAEETREGDVYRKLLDKLEQARQSLGGQVFDVLGKLQFEGKLL
                     RDLLIEAVRYGERPEVKAYLTTVIDHALDRDQLRDLLEDHALAHDAMDASRIQHIRED
                     MERAEARRLQPHYIESFFLEAFRYLGGSARQREPRRYEVTHVPAPVRNRDRLIGIGEP
                     VLPRYERIAFEKPLVAPQGLPLAAFVCPGHPLLDSVIDLTLERHRDLLKRGAVLVDER
                     DTGIQPHVMFYLEHTIQDASLTRSGERRVVSRRMLYIELDADGATHHVNYAPYLDYRP
                     LAKGEPTVEAILNRPECTWITRELEKKAQGHAIVNVVPEHLAEVRGPKLKLIAKTEAA
                     VKERLTKEITYWDHRAEQLKLLEQAGRTGNRLNSGEARKRADLLQGRLQKRLEDLKLE
                     AQISPLPPVVLGGMLIVPKGLLTAMTGQGAAISTAPADTQASAARARAIIMEVERNLG
                     FEPTDRELEKLGYDIESCIPGTGKLRFIEVKGRVSGAPTVTVTRNEILYSLNKPEDFI
                     LAIVEFLDGDSYRVHYLRQPFQREPDFGVTSVNYDFAELLAKAKEPS"
     gene            complement(19284..19886)
                     /locus_tag="PLT63_02800"
     CDS             complement(19284..19886)
                     /locus_tag="PLT63_02800"
                     /inference="COORDINATES: protein motif:HMM:NF015823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqiC family protein"
                     /protein_id="HOD97680.1"
                     /translation="MKKGWPLLLLVALALSGCGVSQSPRFYTLNTLAAPPGPTAAAAT
                     KDAVLIGLGPVAIPDRLNRPQITSLSGENEITVAEFDRWSGSLQEEISRTVTENLTIL
                     LPSYRVVSLPWGRRLNVDRQIAIDILRMDGKLGGQIILKANWAVTVTSDSGTKTILVR
                     HTEIAEGMSGSDYTAYVAAQSRALAKLSREITAAVTSPPK"
     gene            complement(19883..20899)
                     /locus_tag="PLT63_02805"
     CDS             complement(19883..20899)
                     /locus_tag="PLT63_02805"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOD97681.1"
                     /translation="MSKQASMKMIGLFVVGAVVLFTAAVVIFSSGKFFQERKLFALYF
                     DESIRGLNTGSPVYFKGVKVGAVADIRLFYHPEKELLLSAVIIELDQDRVVNLRTREK
                     VETEAKAVQYLVETQGLKAQLDIQSLVTGQLIITLDFYPERPAVFHKYLTEFAEIPTI
                     PTQYEELTRALRDLPIKEMAKKLDKIIEGIEGIVNSPETREGVKSLAQAGKEAQQMIK
                     KMNEQIGPVLADLKATSGAARGAFQQAEKTLAMSEGVSGELAAEAKATLAQTKATLKK
                     VDDNLAAMKRFTQGNTEVAYQLEETLREFSGIARSLRTLTDYLEMHPEAVFTGKKKTG
                     GEKR"
     gene            complement(21036..21842)
                     /locus_tag="PLT63_02810"
     CDS             complement(21036..21842)
                     /locus_tag="PLT63_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOD97682.1"
                     /translation="MTDYFDSKAHIVVEELTMAYGDFVLQRDLTFTIRRGEIFIIMGG
                     SGCGKSTLLKHLIGLKKPARGGIYFHGRNYWQMEENDREKVTRTFGVLYQGGALWSSL
                     TLAENIALPLEEYTELSARQIRKIAALKLALVGLKGFEDYYPAQISGGMVKRAGLARA
                     MALDPEILFFDEPSAGLDPISSRLLDDLILQLRDSLDATIVVVTHELASIFAIADNSV
                     FLDAAAGTMIAAGNPKTLLAEAKDPVVRNFLTRGGSMPVSNPGAIGEGCR"
     gene            complement(21844..22962)
                     /locus_tag="PLT63_02815"
     CDS             complement(21844..22962)
                     /locus_tag="PLT63_02815"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOD97683.1"
                     /translation="MIEFRRPEGGRLVISLQGNWREGGNYPPFAAVQNQLEQSPAPLH
                     VAFDCSGLTGWNSVLITFLIDIIDYAQRHGIEVERESLPPGIAGLLKLAYAVPERAGA
                     RQEEARPPLVERLGQAVYGLWISTAEALSFIGEATMACGRLLRGRARFRCRELLLIIQ
                     ECGIRALPIVSLISVLVGLILAFVGSIQLSMFGAQIYIANLVGIGIFRQMGAIMTGVI
                     MAGRTGAAFAAQLGTMQTTEEIDALKTLGIPPMDYLVLPRMIALFLMMPLLCLYADFM
                     GVLGGFIVGVTMLDLNFLEYYHQTIGAIRLNDLWVGLFMSAVFGVLIAAIGCLRGLQC
                     ERSAAAVGKATTSAVVTAIVAIVIATATITVLCNILGI"
     gene            complement(23093..25669)
                     /locus_tag="PLT63_02820"
     CDS             complement(23093..25669)
                     /locus_tag="PLT63_02820"
                     /inference="COORDINATES: protein motif:HMM:NF022783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3141 domain-containing protein"
                     /protein_id="HOD97684.1"
                     /translation="MARKAAPKKEKAIKIKEAAESVFSPANTKDPTKPVDETAAMGET
                     AKSASRPETKREAPAPTTAAGTAGTGKNEEHREKPQSLLPGFDPAVFFRGFENLWKDN
                     VLLKDLFKSSVAMWQGAIKAPLTPAEVPWGKCLGAYDPAALFQSLEEAWQGNVAAREA
                     WEYLVDTCQRSILFWDVMRRRGDNFLAHVKEGEPPVLQFQYETIIDGRTLDNHVNYAL
                     LRIIPEEGLKIDPLKRPFVIVDPRAGHGPGIGGSKHESQVGIAMRAGHPVYFVMFFPK
                     PEPGQTLVDVGQAEALFLQEVIRRHPEAPRPCVMGNCQAGWAVASLAAMAPDLMGTII
                     LNGAPMSYWAGNKNKNPMRYTGGLLGGKWLTSLTCDLGNGKFDGSYLVTNFENLNPAN
                     TYFRKEYNLYANVDQEAERYLNFEKWWSGYFLMNADEIDGIVTNLFIGNRLAKGGVVL
                     PDGQRVDLRNIKCPVVVFASQGDNITPPQQALNWIVDVYDSDQAILTEGQTIVYTLHP
                     DVGHLGIFVGTSIAKKQHQEFVNTLEMIDALPPGLYEMIIEKKSLFMEHAELIPGEYV
                     ARFEMRKIADITAMSDEIRDEDYFSSVAEVSDYNYELYRMFLGPLVRLMTTEQSAEIL
                     RWLNPSRMKYYLFSERFNPGMVSFRIGADWVRKNRRPIRAGNYFAMLERDMAQTIEDF
                     LDSYRELRDDAYRNLFKTMYGPLGLAPLFPGRQAQVLERENAEREEAIHRKVEELKNR
                     MDQGGFNEALIRLVMAAMIHLGMIDYRSSLIANRLEAKYPKDKISKEQWREMIRDQYF
                     MLLIDEDQAIASLAKLLPTEEEREKCVAMTMEIMMISDARIDPESRLAVKVAKALDLK
                     IT"
     gene            complement(25920..27416)
                     /locus_tag="PLT63_02825"
     CDS             complement(25920..27416)
                     /locus_tag="PLT63_02825"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HOD97685.1"
                     /translation="MVVKPKLTAGPANRYFAFTKRLFVVLVTALLLSGCMLGKDYSRP
                     QVDTPDNWRFEEKDALALANTSWWKQFQDPVLNDLIRTSLQENKDVRIATARVEEYMG
                     KLGVTRADLFPQVGLGASAGRQRLTEVGPTGWNPTTQPTSYTFQGNLNANWELDLWGK
                     LRRATEAARADLLSTDEARRAVIMTLVASVANSYINLLNYDQQLVIARETLKSRKDSL
                     EIFQKRYAGGVISELELNQSRSEYEDARAVIPQLEKNIAQQENALNLLLGRNPGPIPR
                     GRTLDELVLPAVPTGIPSDLLDRRPDVRQAEQNLIAANARIAVAKALYFPSISLTGAF
                     GFASAELNDLMTGPAKTWNYAASLTAPIFTAGKIKGQVKQAEALQEQALVKYLQTVQT
                     AFKEVDDSLVDQRKYRERLAVQGEQVMALRNTRRLAGLRYDNGYSSYLEVLDAERSLF
                     NGQLSQVQTKGSLFQSLVNVYKTMGGGWITEADKMAAGYTETKAAPSP"
     gene            complement(27421..30594)
                     /locus_tag="PLT63_02830"
     CDS             complement(27421..30594)
                     /locus_tag="PLT63_02830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HOD97686.1"
                     /translation="MISKFFIDRPIFAAVISIVIVIAGGVSMFNLPIAQYPEISPPQV
                     QVSATYPGASAEVIAENVASPLELQVNGADNMIYMSSVSTGMGTMSLTVFFDIGTNPD
                     MAQVDVQNRVNIAMPQLPQSVQKQGVTVQKKSSSFLMVIGVYSPDDRYDQGYVANYAN
                     LYVLDALKRINGANRTEILGSPDYAMRIWLKPDRMAQLGITASDVSNAISEQNQQFAV
                     GKIGQSPTDGPVEQTFSVTTKGRLTTPQEFENIILRASKDGSAIVRLKDVGRAILGSK
                     DYSVRTKMNGKTATLLAVYQQPGANAVHVSDEVKKTLEQLKKNFPDGIDYKIAMDSTE
                     FVRASIKDVIITFFEACLLVILVVYIFLQSARATIIPILAVPISIIGAAIGMILFDFS
                     INMLTLFGMVLAIGIVVDDAIVVIENVERNMEEFHMTPNAAAKKAMEEVTGPVIAIVL
                     VLCAVFVPVAFLGGMTGQLYKQFAITIAISVVFSGIVALTLSPALAALLLQHKTGEKK
                     GFFKWFETFFQKITTGYTGGVAKMLKHTKTAMIVFVIMASLTGFMFYITPTSFVPSED
                     QGYLFGVTFLPDTASLDRTEAVGAKAAQFFMKHPAVQDVSEFIGYSLVDSALKANAGV
                     IFVSLKDFEQRQKEELKAPALLHAAFRNFSQIKEGFVMPVNPPSIPGLGNMGGFQFWI
                     LNRGEGDAAKLQNVAETFIATCHKQPELQGVNTTMNAYSQQLRVDVDREKAETLGVPV
                     QDVYEALQILFGSAYVSQFNKFSRLWQVIVQAEPQYRSRPNDLTQVYVRSTSGNMIPL
                     QSVVTMKYESGPDIVNRFNNFPAANIIGGAAPGYSSGQAIVAMERVAKEVLPEDFSYA
                     WSGEAFYAKQAGGTSGLVFVFGLIMVFLILAAQYEKWSLPFGVLMAVPFALFGAVLSI
                     LMRGIENDIYFQIGLLTLIALAAKNGILITEFAVMKRAEGLSIYDAALEAARLRLRPI
                     VMTSIAFILGCVPLAIATGASANSRHSIGTGVIGGMLGATLIAIFFIPLFFQFFETLS
                     ARLEKKTGALAGPVAKKGESTPEKEEK"
     gene            complement(30604..31932)
                     /locus_tag="PLT63_02835"
     CDS             complement(30604..31932)
                     /locus_tag="PLT63_02835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOD97687.1"
                     /translation="MNSANCRNFIPERDFSSKIVILLFCLLLFSGIPWGCGKDQKGGV
                     QRPPAEVNVVTIAPKDTPVVWEVVGQTESSHLVEIRSRVEGFLDKRVYEEGSLVKAGQ
                     VLFRIDPRNFQATLQQAKGELAQQEARWATAKANLARIKPLVEQNAVSKKDLDDATGQ
                     EQATAASVLAAQGKVREAEINLSYTVITSPVTGLSSQAKKQDGTYISASDSLLTYVAK
                     MNPMWVTFSLSENQYLNSLEEIKKDTLRFPPGEKFIVELVLADGSIYPQKGHLIFADP
                     SFSKETGTFLIKATMDNPQGILKPGQFVRVRLKGAVRPKAIVAPRRAVFEGAKGQFVW
                     CVDKEGKAEVRHVIVGEWHGEDIFITSGLKAGDRVIVDGVMMLAEGMPVKIVEKKAPD
                     AKSEGQPVAPDGAGNADPSGGKTATAPPEHKAKDAATAPAAGKNSPPEKK"
     gene            complement(32063..33151)
                     /gene="ydiK"
                     /locus_tag="PLT63_02840"
     CDS             complement(32063..33151)
                     /gene="ydiK"
                     /locus_tag="PLT63_02840"
                     /inference="COORDINATES: protein motif:HMM:NF008216.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HOD97688.1"
                     /translation="MVQSKMKESKRKDVTHITLTVLLIAVLIIAVYLILHPFLPPFIW
                     ATTIVITTWPLMLRAQETLWGKRGLAVLVMTLGLLLVFVVPLWVSLATIADNYRRIDE
                     VAASFCAAAIPPPPSWIQKLPLIGPNLAATWQDLATAGPEGIIARIKPYSGQAAKWFI
                     DQAGNLGKILVQFLLTVIISTILYARGEVATTGIRRFAERLGGKRGEEIVSLVEKSIR
                     GVAMGVVVTALAQSLLGGIGLAAAGVPAAVALTAIMFVLCLAQLGPGLVLLPAVVWLF
                     WSSNAIAGSLLLVWTAGIVSLDNILRPMLIKRGADLPLLLVFAGVIGGLIAFGIVGIF
                     IGPVVLAVAFTLLKSWVEDNRVSQPLDQ"
     gene            33348..34016
                     /locus_tag="PLT63_02845"
     CDS             33348..34016
                     /locus_tag="PLT63_02845"
                     /inference="COORDINATES: protein motif:HMM:NF016301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HOD97689.1"
                     /translation="MPLNPVACPDCDLLQNIPHLPPQAKARCRRCGKVLAINRPRANE
                     RTLALAIGAAIVFTLANGFPLMGLSVAGREVDTTIMGGAWMMWQEGQQMTAALVAICA
                     VIAPAIYISFTIVVLITSRWYRPAPVWVGELLLWAELAHEWAMAEVMMLGILVALVKI
                     ADLATVIVGVGMFATGALIVLITMIAVNFDPEKIWAQVQWVGSVTSEPVGGLRTGGDR
                     EAEK"
     gene            34013..34654
                     /locus_tag="PLT63_02850"
     CDS             34013..34654
                     /locus_tag="PLT63_02850"
                     /inference="COORDINATES: protein motif:HMM:NF016301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HOD97690.1"
                     /translation="MIAGETSAMRAGLALCRHCRLLSRIDTTGSVNYCPRCGHELAYR
                     RHDAIQRTWALIIAAAICYLPANLLPVMKSTTLVGASEDTIIGGVILLYLTGSWHLAL
                     IVLIASVMIPLAKLMSLAYLLITVQRRSTKSNQERVRLYRLVEFIGRWSMLDVFVIAF
                     VVALVQIKPLMAAQPRAGILFFAAVVILTIFAAQTFDPRLIWDFAGNMEKRNE"
     gene            34647..>36090
                     /locus_tag="PLT63_02855"
     CDS             34647..>36090
                     /locus_tag="PLT63_02855"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOD97691.1"
                     /translation="MSETSPAADIPEATTAPRKRARFSVVWIIPIIAALVGIGIAVER
                     FLNEGPTIKISFKVAEGVEAGKTFVKYKDVNIGQVTAVSLSEDYSHVMVTAKIAKSAK
                     NLLVKDTKFWIMHPRITLSGVSGIGTLLSGNYIGIEAGKSKEKEKVFVGLDTPPIITG
                     ADPGRQFVLKAEDLGSLGYGSPVYYRRLNVGQVIAYDLAKDGKTMEIKIFVNSPYDKY
                     VTTATRFWEASGIDVSMGVKGLLVRTQSLASVLAGGIAFETPPYSQAGEQAAENAVFT
                     LFESQEAATTKHEMKDDPFILVFDESIRGLSVGAPLTYMGLEIGEVKKIGIHYDRDSY
                     TFKPYALVSYYEERFFSQVDEKDKDAAVRDHPAKAWQEDQGTQKLIDKGMRAQLRLGN
                     IVTGQLYIALDYFPRAPKVQVDWTKSPPRLPVMPSGMAEFQMKVTGILDKIDKIPFDD
                     IGKETKTALNGLDQTLKDARKLLGRIDAEVV"
BASE COUNT         7610 a        10289 c        10108 g         8083 t
ORIGIN      
        1 tggtgatccg ggcggcctgc aagcccatcc cgtccctggg ccgggaacag acgaccatcg
       61 atcgggacgg caaccccgtg ctgctggccg tccggggccg ccacgacgtc gccgtcatcc
      121 cccggatcct cccggtctgc gaggccatgg tctgcctggt cctggcggat catctcctcc
      181 gccaggaggc gctggggatc acgcggcggg gatgaattca gaggaggagg catgaagacg
      241 gggatatttc ccctcgggat catcggcggc accgggggca tgggcaagtg gttcgcagac
      301 ttcttccgga ggaacggcta tccggtggtg gcaaccggcc ggacccgggg ccccgatttt
      361 gcgtccctgg cggcgacctg tccggtggtt atcgtcagcg tccctatcgc catcacggaa
      421 gcggtcatcg agaagatcgg cccctgcctg cccccggagt ccctgctcat ggacctgacc
      481 tcgctcaagg aagggccggt gcgggccatg ctggccgcca ccgccgcgga ggtcatcggc
      541 ctccatcccc tcttcggccc cggcgtcacc tccctgtccg gccagaacat cgccgtctgc
      601 ccagcacggg gaaactcctg gctgccctgg cttagggata tcctggagaa aaacggggcg
      661 cacctggtgg aaaccacccc ggcccggcat gacgaactga tggccgtcat ccaggccctc
      721 aaccatttcc tcaccgccgc cctgggcctc tccgtcgccg ccgccggcat cgagccggag
      781 gaactgcggc gtttttccac gccggttttt cgggaacgac tggatcatct ccaccgcctc
      841 ctcagccatc ccgatctgta cgcccacctc atcgccggca attcctatgg aaaaaagtca
      901 ataatatcaa cgatcaatca tcttgaagcg ttgttggcac ccgtccgggg gggagatacc
      961 gccgccctgg ccgcacagct ccgggcaatc agcgcaagtt cgccatcccc ccggtcggat
     1021 acgcacgaat cggcagagga atgacttcgt caagcggcgc cgaaccctcg ccctgcactt
     1081 cccggtggac aaatggcata cgcccgacga tctcgtcgtc agttccgcca tcctggccaa
     1141 ggaatacgcc cggctgtcct ccacaaccct ccggcgggat ctgacggagc tggataggct
     1201 tgatttgatc gtcagggaca aggacaaata caaggggaac atcgagatca tgcgcggata
     1261 catgccgcag cggaaggcaa gaccggtccg ttaaataacc agcgacacac caaaagagta
     1321 aaaatcacta cttgacaaca tcgtaacaag aagtccatat gcgtctgaag caagaaagcg
     1381 gctatacaat ttggtcgacg aggttaaaga atcacatacc tccatacaag tagtaggcaa
     1441 gagaggttct gccgttctta tatcggaaga agactggaga gctattgaag aaacacttaa
     1501 tcttacatct attccgggca tgagggagtc catcaagaaa gggctcaaga ccccggtgga
     1561 aaaatgttct gaggaacccg gctggtgaaa tggcgattgg tttttacgga acaggcccaa
     1621 aaggacgcaa agaagatcgc cccatccggt cttaggccgc aagtatcaaa gcttttagag
     1681 attcttcaga atgatcctta ccaaaaaccg cccccctacg agaagctggt gggtgatctc
     1741 accggcgcct tctcacgcag gacaaacatc caacaccgtc ttatctatca agtcctcgtt
     1801 gatataaaga ctgtgaagat aatacgcatg tggacgcatt atgagtgaat gaaaaacggc
     1861 gcgcattgtc gtcaattcgc tattaatatc atacatttaa cctctgccgg tgctccggca
     1921 aagattttta tccgggggaa aacgagccga aaactccggt tctgaaattg gatgacatcg
     1981 taaaaagcgc aaaaaatgtt ccatttggga cggcatctta aaaagatccg caggcgaggc
     2041 gcgcgaatca tgaggaatga ggcgtactgg gacgtacgtc gcagtgacga gggatgaagc
     2101 gctttcgaag catccggact ttttacgatg ccgtcaaatt gaatgattga gcaggattta
     2161 atatcgcaat aactgggaaa atattccatc tatatgcgct gatatgatgg aatattttcc
     2221 gtctattaca ccgttcggta tgtgaaggtg atcgagatga atccagatgc aatccgggag
     2281 aagtttgggg acgactacat agcagatgaa cgtacgttca tgatgggcat tgatcagcga
     2341 ttcaccgcac atatggcaga gcggtttcgt gggcgcagag tccttgagac atgcacgggt
     2401 gctgggttca cgaccatcgc tttggctcga gcagcatccc aggtaataac agtggagatt
     2461 gatccctacc atcaaaccca ggcaaagaag aacttacaaa gagcaggtct tttgagctcc
     2521 gtggaatttg tttccggcag tatcctcgac ccaagcctct tggcgggact tccgcccttc
     2581 gatgcagcat ttctggatcc ggactgggcc ataactggcc ctgaccacgt ttatcgtttc
     2641 gttcactcca acactcaacc accagcagat atactgctca agacaatcct tgagcggact
     2701 cgaaatgtag ccattgtgct gccaccactt ctggatacac gggagctggc ggggctgcct
     2761 cgaaatgaat gccagaagct ttatctgggg gagagccacg agttgtattg cctttatttt
     2821 ggggacttgg ctgctggctt tggtgacacg gcattccgtg tatcggtgtg acaccggcat
     2881 tgccgaacaa ggtcatgcct ctgtgcttcg cgcccacaac gttagcgcaa ggagaaaaac
     2941 ttcatggaat attcaatcag gccgatatca aatgaagatc gtgaatcaat aatggacatc
     3001 tttaaccatt acgtagaaaa ctcctttgcg ccatacccgg aaaataaact tccatatcag
     3061 gcctttgata tgtttcggca aatgtcacat ggcttcccga ccggttctat aaaggatcag
     3121 aatgggaaaa ttgtcggatt tggaatgcta cgcgcacata atccaatgcc gactttttca
     3181 caaactgccg agacgacata ttttatccat ccagaacata caggaaaagg gaggaacaaa
     3241 gatgaatgag cggcacagca agtggcaaac caaagaaata gcagagacct ttgtggaagg
     3301 tgttcgcgga gctatccctg gtgccaaact ccagttggaa gtgatcagta agattataac
     3361 tgcctggttt cctcagccat ccaggattct ggacctgggt tgtggcgatg gaattatcgg
     3421 tcgcctgctc cttgaagagt atcctgaagc aagggtgata tttgccgact tttccgaacc
     3481 tatgctggaa aaattacgga taaaaatcgg cattaatcag cgtgcaaaag tcatcaatgc
     3541 ggatttcgca acgtcggact gggttaaggg tagcgagtta gaaaaacctt ttgatatcgt
     3601 cgtctcgggg tttgccattc atcatcagcc ggatgagaga aaggttgagt tatacaccga
     3661 gatcttttgt cttctcagaa gtggcgggat tttcctcaat ctcgatcagg taaaatcaaa
     3721 aaacccctcc acaagtgaaa tatttgatgg tttttttctt gatcatattc gacgcgctct
     3781 ctcaatatcc gatcagaatg aaatgatgaa tcaaattgag aaggcatact atgaagacaa
     3841 aaaagagaat atccccgccc cggttgaaaa acaatgccaa tggctcagtg acataggctt
     3901 tgaagatgta gattgcttct tcaagacatt tgaacttgct ctatttggtg gcaagaaaac
     3961 attcactcgg gtagccgggg gtagctagcc cccagccccc caccacccgg catgcgggtc
     4021 cgcaccgggc ggcgaaccga gattaccggg ccgcggccct attcatgagc ctcatcaaat
     4081 cctgtaagga ttgtaacgtc aatccctggg aatacttcga cgacctcctc cagaacatca
     4141 tgagccaccc gtccaacagg ctccgggaac tgctgcccga ccagtggaaa cctcagacga
     4201 aagagtaacg cggcttgatc ctgaagcccc gccgatctgc aaccgccggt ccgcgcttcc
     4261 cgatcgccgg ccgtctgctc cagcccggtg actgcgacca tccgccatcc attttcaacc
     4321 cagattttcc attaggagat tttagccata ttgatgaggc ggcaatgata gaggttacaa
     4381 agattaccgg gacatgttct aatggaaacc attagagata ccttttcaat tacggcatgt
     4441 tacgggaggg cagagtgacg cgacagcttc aagggggtgg ttttacggtc ggattttcgg
     4501 acagggaggc tacggcatgg ggcggtttgg cgttgatgaa gcagatgctt gatcgtcttc
     4561 agttccggga agcgtttccc cagtttggtg taccggaggc ttcttcaaac cgtggttctg
     4621 taccatgcca gcttattgaa cagttcattg tatcgatctg gtgcggggcc tgccggtttg
     4681 cacatgcgga ggtggtgcgt ttggataatg tgctatgccg tcttttcggg tggtcatgcg
     4741 ctgccgggca tggttcagcg gcctgtgggc caacgcctcg aacctatcat gttgtaaaac
     4801 ctaaactaca aattatctaa tggaaaatct ggatttaatg aacatcaaac ttcgctttca
     4861 ccccccgttg tggccctgcg ggaattttga cgcggccttt gtcggagggc aaaacttttt
     4921 ccgcgtcgtc taaaactcaa taatatcaaa tagattatat atgtcggagc tccgacatac
     4981 cccctgaaaa agtcccttca ccgccggtgt aaaaggacaa atatccgaga tgctgaggat
     5041 atattctcgt atgccgtcat gaccgtttca ccttaattaa ttgataagac gcctgtccaa
     5101 aatacctgga gccaaaatac tgcgccagac cccttctatt ccttctcaaa ctcctggctc
     5161 gcgaatttca gtgtccggct gttttcggtg acggtgcgga ccacgtgatc cggagcaccg
     5221 gagggaatat ccgcctcgac ctccagtgtg acagttacag tggcaccaac cagaccggag
     5281 agatgggcaa tcacctcatc ggcaatacgg ctggcgtcgc gtccgacgcg cgccgcatcc
     5341 agaattaccg tcccgtggaa gcgcttcagt ttcggcgcta ccgcaggtgt gtctggcgtt
     5401 cccatgggac cacctacagg tataatagtt gtcgtccctt ctccacccgc agttggtccg
     5461 acagtcacac cacccacagt cgttttcgct ttctcttcgt ccatctgcct acaggccaca
     5521 tccggtttca ccagcaaccc ctgcgtctct cccccggcaa tgtaaacctc cacaccgcag
     5581 cgaagccccc tgtatcggtt ggcgctttca tcatagctgt cggcaaaggc aaaggagtcc
     5641 tgcatccaca gcatcaaggg caacccggtg cggatggcgc cgactaagac cgatgaatcc
     5701 ttgagtctgg gcagatagct gaagctcgca aagtcctcgg caagctgctt gatggcaaca
     5761 tggttgccac gccagagggg aatcctgtcc agctccattc gcagccgtgt tgctgcaaag
     5821 gtcgtaatca gcaattcatc gttcttgagc ttcttacttg cccgtacggc cagtgcatcc
     5881 tgcccggaaa ggcgcagcgc ttgccattcg acggccgatt gcggcgtcga ttgcaccggc
     5941 accagcagcc actggtatgt ttccggcagg cgggccgtca cggcgctgtc ggccgcagtt
     6001 ctctgtgttt cggcttgctt tacctgttgc ggatcgaggt ttagtccttt ctcccctttt
     6061 tcagtcagga ttgattccca ggcgagaaat ttccgggccg cctcgtcaag gtcctggaga
     6121 cgtgtcttgt cggcggcaag aaaaacgagg gtgttgcgat agtggcgcgg ggagctgccc
     6181 cgcgattcca gaatcgcctt tgcggctgcc tctgcggcgc ttcccggctc cttgctgtag
     6241 ggatgattga tccccaagac aaccagacgt gcgtcaaggt cgtcggaaac atcttgcccc
     6301 gattgtggca ggggatggat acggctaaag tcacccttgc gttccagatc cttgcgcagc
     6361 cgtccgtcca gttcctgcat caccttgtcg ggatcgcgct tgagttgttc agcgcggtcc
     6421 tcagccaact tggtgaccgt gggctgggtc gaataccagt atcgcggacc atcctggtag
     6481 agataggtgg cagcggcagc caatctccgc agggcgtctc cgaaaacgtt tggcgattct
     6541 cccggcatga cgcatccgag cttcacctgc cggtcctcaa ggccgcgatg ggcggctttg
     6601 gtcaggggcg ctgaccccag atagatggca cgggccacac gtcggcaggc cgcaaatttg
     6661 cccaggttgg gcacttcacc atcgatttta agcggcagcg agttcggacc atccacgtct
     6721 ctttcgatga tcggcggcca gttgtccgaa agatagcggg tcagttcaga ctggacgcgc
     6781 gaatcatcga tggagatatt cgccggcatg atgaggggat tgcggtcgcc cttctcccag
     6841 aggctgtgga tgacggacgc catcaatcgg aggacgcccc gtgtgcgctg gaacttcacc
     6901 agggtggacc agtcggtgta gaggcggtcg aagatctccg gatggatggg gtaggctgcc
     6961 ctgatacgtt tttcgtaatc ggcgtcgcga cattccgggg ggaattccgc ctgctgggtg
     7021 cggtagaagt cggcaaatgc ccgtgccacg acgtcgcggt ccttgaactg ggccggatcg
     7081 gccagtggtt cgaagagccg ccgcctcacg atttcgaaac cttcctcggc actggccggc
     7141 cgccaggaag attccagccg ccccacaaca tttctcaacc tgtcgagggc ctcccgtccc
     7201 cgctgaccgc ccacctctgc gtcatccgtc tgtgcgtggg gcgagcccga cgtatccgaa
     7261 gcgggaaggc tgatcaccag cagacagttc cccgccagtt tcgctgattc ggtcaatgcc
     7321 tgggcaaagg tgaattgcgt ctcgaaactg ccgccgggaa gatcgctctg atcatgaagc
     7381 tgacgcgcat aggcgaccca ttcgtcgata aggatcaggc agggaccata cctgacgaat
     7441 aactcacgca gcacatcgcc ggggctggtg gcccgctcgt catcggcggc aagctgatca
     7501 aatgccttcc ctgcctcgct ctcccctccg gcagcgtagc ccaactgcca ggccagctcg
     7561 ccccataggg tccgcaccac cgtgccgtcc tgtttcctga ccggattgcc gggggatatc
     7621 cgattgccca ccagaacaac gcggcggacg gacggcagtg tcgccgttcc cgcctcctgc
     7681 atgacggcat cgatgccggc aagctcggtt ggggcaatcc cggataacag gtgataaagc
     7741 gccagcatcg cgtgggtctt cccccctccg aagtttgtct ggagctgcac cacgggatct
     7801 gcgctctgcc cggaaagccg ccggaccgca ccgacgagca ttcttttcag gctttcggtc
     7861 agataggtgc ggcggaaaaa ttctatggga tctcgatact caggggtgcc ttcgcccaga
     7921 cgaacctgcc agagatcagc ggcaaactcc gcctgctgat agcgaccgct ggccacatct
     7981 ttgtgtggcg tcaccacttc gcgccagggt ttcagatttc ccgccgcagc gctttctatg
     8041 gccgatcccg cgctttttcg cttttcgctc cgcacctgtt cgtcgtatat ggtgcgccgt
     8101 aattccatct tgagttttcg gacgtcttcg gcctgcggcg ccgataccgc agccagtaac
     8161 cgctcggcgg aatcaagcgc ccggtcggcg tcgtcgccgg aaaatggttc ctggtgagcc
     8221 catttgttgc gatgatcgcg cagttcgctc accaggctcc gttcggtgtg cccaagcgtc
     8281 tttttgaaga cgctattcca ttggttccaa agtgcagcca gcacgacggc gatatcgccg
     8341 aagggttcgg cctccgtacc ggcaaaactt aattgctggg gaggaagaga cgctttcagt
     8401 tcgtcaaacc agctttgttg atactgggac tttagctccc tctccacgaa gggacgcaac
     8461 ccctccttta acaggctaag cgccttaccg acgcgctcat gatttgtaat tgccataatc
     8521 tcattcctgc cttcttctca agcgaacctt cttgcctgta gtgctcttga gcattcgttg
     8581 tatcccttct ataaatacct ggaaactacg tgaatagtta tctgatttct caagcggcat
     8641 ggccatgccc atccggcggg caccctcaat ttttctgaat tctggtatca gtttaatcac
     8701 ttcggaagat gggttgccga gtcgatccgg atttcgatac ttagacttta aattcaaacc
     8761 ggttaacttt ggtcgggcat agaccgccgc cagtatatcc attgcgccta aataccatga
     8821 ctccaattcc tggcaggcaa tgcgaatcag aacatctgat ttcccttcgt tcttgcagac
     8881 atcaattaat ctcgctttga tggcacggca atctgccccg tcattatcac ggataatgac
     8941 aaacagctcc cggcgaaatg ccttaagctt gcgagggatg cttttctcca gatcctgctt
     9001 gccctcatgg gtgatgcaga ggaattcaag atcggggata aggcggggca agaactcttc
     9061 gataaacgcc ttcatcgaag gttcctccag aaggaatacg atcttcttca ttattaccat
     9121 gcccccttga aaacgccttg acgccaaaga gtcccgggat aatcgccttc ggcaaccaga
     9181 cttttcagga tctcactatc ggcgccgcga tgaatagtcg cgtaaccttc cttcttggtc
     9241 aaccagtata tctcgtccag attcagacca ttcataaact ccggggaatg ggtcgatatg
     9301 aaaacctggc cgcccctccg ggcgtaatcg cgaaattctt ccgcaagctc ctgcaatagt
     9361 tccgggtaaa gttgattttc cggttcttcc accgccagca gcggaaacgg attgggatca
     9421 tgaaggagga ctagataggc aaacatcttg atggttccgt ctgatacgta gcgagcaata
     9481 aagggatcct taaaagcccc atcctggaaa cggagcacca gccgtccgtc ctctgtaggc
     9541 ttggcttcaa ccttttccac accgggaatg cgcctcttca tggcatcgag aatatcatta
     9601 aaccggtctt ggtggtgatc gaagaggaac tgtgccacct gcgcaacatt atcaccccgg
     9661 gtggaaagat gttcggcata cccttcctcg gcgctgggac gggcgtcggc aatgtggaaa
     9721 tcggtgatat gccagttctc aatgagattg cggaactcgg agacaacgcg aaactccttg
     9781 aactgcccca atcccttgat cgccaggact gtcggttcat caaggatata ctccttacgt
     9841 tccttcttgg cccccggttc accatacacg gcttcattgg taatggctgt ccccttccca
     9901 cgggaaaaat cgacaaaatg ccagggttga ccgcgctgcc ccctgcggaa cttaagcact
     9961 tcccggtcca caacaacctt gccgccttca aatgctacgg atagttcata agttgccaat
    10021 cgcttgcctg tttcccgaaa tttaagcaca atggatatcg gaccttctgt accccggcta
    10081 accagttctt taaacccgcc acgtttggca acagcttttg ctacgttctg tgcaagcgca
    10141 tccttcagga aggaaaaaac atcgaaaaaa gtggatttcc ccgctccatt ctcaccgaca
    10201 agcacggcaa gtctttttag gtgcatcaat gaggcctctt tgaacagccg gtaatttaaa
    10261 atgtcgatgc tctcgatctg catgctctgt atttccatcc tatccatggt tctttctccc
    10321 catcatctac aaaataacct atatttcgtc attgtcgaag atatcggctt gcacgcctcg
    10381 cgatttttca cttcccgttg ccaaccgcat gatttctggc cagctttgca ccaggctgtt
    10441 gtatgaaagc gcctcggcgg cgcgtttctt ccgttcgcac aatgtataga gtcgatacgc
    10501 caattcacgg gcaacttccg ctttcgatcc cagcttggtg acaagaaccg ccgccgcgcc
    10561 ttcaccgcct gtttcaagcg cacggacaag ctgatggacc atctcccacg ccggaatccg
    10621 tttgtctttc tccggatccc aagcagcgga tagttcgtcc ggacggagca gtcgtaccct
    10681 gccgccagta gatgcgagta ttccgtctcg cttcatcccg tcaatgctgg tggccttggc
    10741 ctttgagagg acctccgcca caccgtactc gccttcgtca aagcctgctt gctcgaacca
    10801 cgccaatgcc cagcggctgt cggcatcaaa gtcgccttcc tgctcggcca agacctcatc
    10861 gagggtttga ttgatcagcg ccagggcctc ccgcaccgta agcggcttgc cctcggcatc
    10921 gaggactttg gcgtagcgag tgaagattgc catccccggg ccgatggccg cctgcgccaa
    10981 gtctaccgga gcgatattgc tacgctgcaa atgggcgagt gccacgggta gctccgcttt
    11041 aagcacgttg atgaattcgc gacgtgtgga tattggcgcg tctgctacgc gcttacggca
    11101 taccagaaca atgctggagg caagggcgtt tgatgcctgc cctctcaagc gactgtcgtt
    11161 ttctgttcgc attggccatg tcccggtggt agagaaaccc gcacaaatca ccgcctctag
    11221 gaaagtctcc caacctgtgc tcgcgtttcc atcagcgcca tcgttttcga cttgtttgaa
    11281 agcataatag atggtcaccg gaaatgccgg atgcgactgc tcggcaaggc gctgcatcgc
    11341 ttgcttcata ccattgagga aaaacgcctc tgcatccacc ttaccaccgt gacgataacc
    11401 gaaggcaact aattcttcag ttttaggcac agcaagcgta gcaaagagat ctgggaagac
    11461 aggctgtaac gatctgcgca accagacgta gaagaagtca gagagatcgg catagggtac
    11521 gttgtcgtag tacggcggat cggtggaaac gacttttgct ttagacgcat cctgccgact
    11581 agcatccgcc tgtagggcag accctatcac tttcgacgaa acaaactcga aggctttagc
    11641 gaacgccttc gtaatcccat caatcaatac gatccaagca ccagagctgt cgccgagcgg
    11701 attcccttcc gcaaaatccc agaccattgg gatggcttgc cgtccaaata gctgacgagg
    11761 acactgtgcg atgggttccc acctgcagag actatttcca aggtcggcct gtttgctcag
    11821 ggcgaaagat agataaacac ctacggcatc agcgtaggat atgataccaa taccaggatc
    11881 gaggaatttg ctatcatcca acaggcccgc ggcaagtgcg tcgcgcttta tcatcgcgca
    11941 cgcctcctgt accaagttgg ataagaccgt cagcgtcacg agttgccggg cagtgaaaag
    12001 gtcgtaccat ttcgccagcc catacagggt aggcgtaaaa gcccgtgcgt cttcgaccaa
    12061 agtgccttct ggctgccacg ccggattcgt ctgacgcgct acagactcca ttgccgcggt
    12121 tggcgggagg taaatccgcc cgcgatcacc cgcagcaacg attgccatca tctgcacacc
    12181 catgcgcccc gccttgccct cagcttttat gtaatcagcg gcaatcggag tcccagacat
    12241 tatgcattgg aaatgcgcgc cgcgtgaaag ctttgtacca ttcttcgcag cctcggcatc
    12301 cttcggcttg cccaccttca ccgtgaagcg gtaaccacca ttctcgatca ccggttcgac
    12361 ataggcttcc ttgccagcct tggctgaaag catgaaagtg gaggcgagcg gcacatccac
    12421 attggcaaag gctgggttcg ggctctttac cgttcgtgcc catagccacg caatgacggt
    12481 gagcttcttt cccaaatagg gtttgagatc cgagcgttct ttcaccatct ctgtcgtaat
    12541 ctcgaccttg gggtagagat ggccgatgcg cttttcagcc tcgtcgcgca tccattttcc
    12601 gtaataacgc acgtcctcgg caaggccctg tgcgcctttc catgtcctca gggctttttg
    12661 cccatccggc aactgctgcc attccggatt gaccggcggc ttgccggcaa acttgggcgg
    12721 aatttcgatc atcgccttgt tgatcaggac cgccacagga tttaggtcac tggcatacgc
    12781 ttccaaaccg agccgttgcg cctccagcgg cagtgcacca ccaccggcaa aaggatcatg
    12841 aaaagcaggt agcttgtggc ggtcaaaaag ttccttggcg cgcgggtgac cggcattttc
    12901 agcacaagta tagcgccagc tttgccagat ttcgtctctg gcttgctgga gaactttctc
    12961 attggtcgtg ttttcccaga gcaccaggtc ctcgataatc ttgaagagac gttttctctc
    13021 tttttcctgt gccttttctg tggggaaaag atcaggatgc gccgaagggt cgtccaccat
    13081 ctgcgaaaag atcaccgccc gcgccgccgc caagggccgc cgcgcccacc agaggtgcag
    13141 ggtgctcgga tggccgtggc gaatggactt ctcccgggcc gacgccttgt tgatggcatc
    13201 cagcggcagg gcaacttcga tgagtttctt cttgtaattc atgatctttc cttatgactc
    13261 cggtcgaaaa tctgacgatt agcgtcattc atcggacgat tatccgacga ttaaggcaga
    13321 atatcggtcg attccgagga aactcggaag gcggtgaaat cggaagatta cggcaaaaca
    13381 tcgaaagatt atcggaagat ttcatcatca aaagccactt ccattttgag cttaaaatct
    13441 tccagcattt tgccggattc tttcaaggtc gtactgtcaa cgatagcctc tccgcattcg
    13501 gaacatgcat aggtaatgta attcggaatt acctttgttt gccctttata ttcaaaggac
    13561 tcggtttcaa ctttcttttt cagcttgccg gctccgcaga taggacaatt ttttgttttc
    13621 attttttttc agatcacctt ccaccagggc accgccaaaa ccctgggcgt caaccactca
    13681 attaccgtgc cggtgtgcag cagtaacccg gcacgtgcgt cagcaccata ctcattttga
    13741 aaggcaagca ggttcatggc atccttgatc cgcgggcggt ttgtggattt gatctcgatg
    13801 ggaagcactt tcccgtccgt ctcgatcacc agatccactt cttcacctgt cgtcgtccgc
    13861 caatagagaa tctccgtatt ctggagcctt ccacactgcc agacaagaag gtcaagcaac
    13921 acaatgttct cgagataggc gcccgttggt tcggccattc cagagaggtg catcgccagt
    13981 ccgacgtcgc cccagtacag cttgggggat ttgaggagac gtttggtacg gttcaccgaa
    14041 taggccggca agcgaatcaa aaggtacgaa gtttcaagca ggttcaggta gcggtgtatc
    14101 gtgggctgct ttaatgatag atcgcgggcc atttccgtct ggtttacgag ctgcccgaga
    14161 cgaaaacagg cggcgcgcat cagccggcga aaatcaggca gggcggctat ggaggaaagc
    14221 acctggaggt cccgctccaa gtaggtccgg acatagccgt cgaaccagac catccggtcc
    14281 tccgcacggg tcatgtggac agcgggcgtt ggaaatccgc cgcgacgggc aaatgccagc
    14341 cagtcttccg gacccgatgt gtccacactg agtagatcgg gccattcggc gtcattcgct
    14401 gcgaccagtt cttcccaaag tccgcaacgg ccgaggccca attgctcccg ccgggtcatc
    14461 ggccaaaggg tgaggtagct ggcgcgaccg gcgagagatt cggatactcc ctgcatcagg
    14521 agcaggttgg ccgatccggt cagaagaaat cggcccggtt ggcggtctga atcgatggcc
    14581 cgtttaacag cgaggagcaa atcgggttcc cgttgcacct catcgagagt caccttcgcc
    14641 gcacccccca ccagggcttc aggattttgc ctggcgagtt cgaaaacatc catgtcatcg
    14701 agggaaaagt aggaacgctt ttccagggta agcaccttta ccagagtgct cttgcccgtc
    14761 tgacgggcgc cggtaaccac aacagccggc atcacacgta accgttggcc caatgcggcc
    14821 atcatgtgtc ggggaaatgt attttcattc atggcgtgaa ttatatccat tcatggacga
    14881 ttttgcaagg attatgaggg ttctttcgct ttggccagca actcggcaaa gtcgtagttg
    14941 acgcttgtca cgccgaaatc cggctcgcgt tgaaagggct gtctcaggta gtgtacccgg
    15001 taagagtcgc catcgagaaa ttccacgatg gcaaggataa aatcctcggg cttgttgagg
    15061 gagtagagga tttcattgcg ggtcaccgtg accgtcggtg caccggatac tcgtcctttc
    15121 acctcgatga aacgcaattt tcccgtgccc gggatacagc tttcgatatc gtaacccaat
    15181 ttctcaagtt cgcggtcggt cggctcaaac ccgaggttgc gttcgacctc cataatgatg
    15241 gcgcgggctc gggcggcact ggcttgtgta tcagcaggcg cggtcgatat tgccgccccc
    15301 tgacctgtca tggccgtcag taaacctttg ggcacgatca gcattccacc caacacgacc
    15361 ggtggtagcg gcgagatctg agcttcgagt ttcaggtctt cgagtcgctt ttgcagccgc
    15421 ccctgaagca gatcggcccg cttacgggcc tcacccgaat tgagccgatt acctgttctg
    15481 cctgcctgtt ccaagagttt gagctgctcg gcgcgatgat cccaataggt aatctccttg
    15541 gtgagccgct ccttcaccgc cgcttctgtc ttggcaatca gtttgagctt ggggcctcgc
    15601 acctccgcca agtgttccgg aacgacgttc actatcgcgt gaccctgtgc cttcttctcc
    15661 agctcgcggg taatccaggt gcactcgggc ctgttgagga ttgcttcaac agtcggttcg
    15721 cctttggcca gcggccggta atcgagatag ggtgcataat tcacatggtg tgtggcgcca
    15781 tcggcgtcaa gctctatata tagcatccgc ctggagacga cgcggcgctc tcccgaccgt
    15841 gtcaggcttg cgtcctggat agtatgttcg agatagaaca tcacgtgcgg ctggataccc
    15901 gtgtcgcgtt catctaccag cacggcgccg cgtttcaaga gatctcggtg gcgctcaagg
    15961 gtaaggtcga taaccgaatc aagcaacgga tgcccaggac aaacaaaggc agccagtggc
    16021 agaccttgag gcgctaccag cggcttctca aaggcaatgc gttcatatcg gggcagcacc
    16081 ggttcgccga taccgatcaa acggtcacgg ttccgcaccg gggccgggac atgggtaacc
    16141 tcgtagcggc gcggctcacg ctgtctggcc gatcctccca gatagcggaa ggcttcgagg
    16201 aaaaaagact cgatatagtg cggctgaagg cgtctggctt ctgcccgctc catatcctcc
    16261 cggatatgtt ggatgcgact cgcgtccatt gcatcgtggg caagagcatg gtcttcaaga
    16321 aggtcccgga gctggtcgcg atccaaagca tggtcaataa cggtggtcag gtaggcttta
    16381 acctctgggc gctcgccata acggacagcc tcaatcagca gatcccgcaa cagtttaccc
    16441 tcgaattgga gcttgccgag cacgtcgaat acctggccgc cgagggattg acgggcctgt
    16501 tccagtttgt ccagaagctt gcgataaacg tctccctcac gggtttcttc ggccaccagg
    16561 ttccagagat ggcatacctc ggtctggccg atgcggtgga tacggccaaa acgctgctcg
    16621 atgcggttgg ggttccacgg caggtcatag ttcaccatca aatgtgcacg ctggaggttg
    16681 atgccctcgc cggcggcatc cgtggccaac agaacctgca ctttggggtc atgcttgaaa
    16741 gactcctgct ccttcatgcg ttcctcgcgg cccatgccgc cgtggatcat aaccaccgcc
    16801 tccttacgtc cgagcagcgt ggtgatacgg ttctcaagat aattcagggt gtcgcggtgc
    16861 tcggtgaaga gtacaagctt ctgttgtaag gatggtgtgt gacggggaat cgctccggcg
    16921 ccgaaaggag taatcggttc ggctaatctg ttcgaaattg cggcggtggt gaaaatctca
    16981 cccagaaggg ttgaaagctc gcgccatttg gtatccgtac cgctgcgacg gacaccgagc
    17041 gcgagcgact ccagaccttt gagggtctca atctccgctt ttaactcggt gatggttcgc
    17101 gccgccgttg cctgatcgag gatttcctcc tcggcggcct tgacctcatt gtccggtgca
    17161 tcttcgagat cctcgacgtc ctcggcatcc agagccagca ggtcggcggc aaggatcggc
    17221 gcggcttggc ctccgcgttg gagcacttcc atctcacgca gtctgctttc taagcgttct
    17281 cgtcgtctac gcaatgattg atagatcgct tcgggcgagg aagcaagccg tcgttgcaga
    17341 atagtgaggg cgaagcccac agttccggta cgtttatcat tctgtagcgc ctcggcacgg
    17401 ttgaattcct cgcgaacata ttcggtgacc gctttgtaga gcttcgcttc ggcctccgag
    17461 agtttgtagg gaacggtgta agcgatgcgc tcagggaata gcggcgtacc gtcgaacttg
    17521 agcaggctct ccttcaccat ccggcgcatc aggtccgaga cgtcggctac atgaaccccg
    17581 tcgcggaagc ggccttcgaa acggtcgccg tcgaggaggg ccataaagag ctgaaagtca
    17641 gcctccttac cgttgtgcgg cgtcgccgtc atcaagagga agtgccgtgt gagggtcgaa
    17701 agaagctggc cgagccggta tcgcttggtg tatttagtct ccgtgccgaa aatggtggca
    17761 gacatcttgt gcgcctcgtc acagaccaca agatcccagc gacaatccgg cgtctggagt
    17821 ttttgctgta cgtcctcatt gcgcgagagc ttgtcaagcc gggcaatgac aaggttggtc
    17881 tcaaggaacc agttgccggt gcgtgccgct tcgagcttgt cgttggtgag aatctcaaag
    17941 ggaagatgaa atcggtggta gagctcgtcc tgccactgct cggcgaggct gcccgggcag
    18001 acgattagac agcgctgtaa atcgccgcgg gcgaccagct ctttgatgag caggcctgcc
    18061 atgatcgtct tgccggcgcc gggatcgtcg gcaaggagaa agcgcagcgg ttgacgcggc
    18121 agcatggcct cgtaaacggc ggtaatctgg tgcggcagtg ggtcaacaat agaggtgtgg
    18181 acggcgagca ccgggtcgaa gaggtgggca aggcggatgc ggtgcgcttc ggaaaccaga
    18241 cggaacagcg ctccgtcgcc gtcgaaactc caggggcggc cttgctccac gagatcgaga
    18301 cgtggctcgt cgtatcgata gagaagttcg tttgccacct tgcccgtggg cgtcttgtag
    18361 gttaattcga gcgcctcgga gccaaaccac tgcacgctga caaccgtgat aaggcagtcc
    18421 ggaagaaggc cacatactgc ggcgtttggt tgaaggtctt cgagacgaat cattcggatt
    18481 tttatttagc agcgatgttc cggcgtgagt tcctgatgtc gttcttactt gcctgatgaa
    18541 agtattcttc aaaacataaa accccgctct cttctggaga cacggggttt cagtaatcag
    18601 tccatagcat cctcacgctg tgttgaccag tttaggattc ttaatcttta tagtcacata
    18661 tgcgacaccg attatcataa tataattaag attgctacag aaaaacgaga aataagacga
    18721 aggactcttc aagaggcaaa gaccgggttc tttatgaaaa taatagtagt cagacatgtg
    18781 cgtctttcga accgcattca atatttttat caaaatggcc aagcattgac ttgtatttgc
    18841 tttcagtaag gctgattttc aattctgtat cgtatgccat gcctcccgtt catctacgag
    18901 tcaataaaac tacgactcct gaaaagcaat gaatacgctt attcgaaatg gagcaatttt
    18961 tttatcacgg gaggtgggta aagatctgta cccaatctta tttgacgaca tcgtaaaaag
    19021 cgccctagcc cgccgactgc cgggcctgta taaaagaagt aagtcgagtc tgtgccatga
    19081 aaccggccca aaaccgtcaa acacgtatct aaagaatcca attagttacg tgcgacttac
    19141 ttagaagtcc atatgcggcg ttgcgcttca tccttcgtca ctgcgacgta cgcacaagta
    19201 cgcctcattc ctcaggattc gcgcgccttg cctctggagc tttttacttc gtcgtctcca
    19261 tcatgacttt ttacgaattc atcttatttg ggtggggagg ttaccgcggc ggtgatctcc
    19321 cggctcaact ttgccaatgc ccggctctga gccgccacat aggcggtata atccgacccg
    19381 ctcatgcctt cggcgatctc cgtgtgccgg accaggatgg ttttcgttcc gctgtcgctc
    19441 gtgacggtga ccgcccagtt ggccttcagg atgatctgcc cgccaagttt gccgtccatc
    19501 cggaggatat cgatggcgat ctgcctgtcg acgttcagcc tccgtcccca gggtagggaa
    19561 accacccgat acgagggcaa gaggatggtg agattctccg tcaccgtccg ggatatttcc
    19621 tcctgaaggg atccggacca gcggtcgaac tccgcgacgg tgatttcgtt ttctccggac
    19681 agactggtta tttgcgggcg gttcagccga tccggaatgg cgacaggtcc gagaccgata
    19741 aggacggcgt ctttggttgc cgcggcggct gtcggtcctg gcggcgccgc cagcgtattc
    19801 aaagtataga agcgcggtga ctgcgaaact ccgcagccgg agagtgccag cgccaccagc
    19861 aggagcagcg gccatccttt tttcatcgct tttcacctcc tgtcttcttc ttgccggtaa
    19921 agaccgcctc gggatgcatt tccagatagt ccgtcagggt gcgcagggag cgggcgatac
    19981 cggaaaattc ccgcagggtc tcctccagtt gataggcgac ctccgtattt ccctgggtga
    20041 atctcttcat ggcggcgagg ttgtcgtcca ccttcttcag ggtggccttg gtctgggcca
    20101 aggttgcttt ggcctcagcg gccagttctc cagagacccc ttccgacatg gccagcgtct
    20161 tctccgcctg ttggaaagcc ccgcgggccg ccccggaggt agccttgaga tccgcgagga
    20221 ccggacctat ttgctcattc atctttttta tcatctgctg ggcctctttg ccggcctggg
    20281 ccagtgattt gacgccttcc ctggtctccg gggaattgac gatgccttca atcccttcga
    20341 taattttgtc gagtttcttg gccatctcct tgatcggcag atcccggagc gcccgggtca
    20401 attcctcata ttgggtggga attgttggta tctctgcgaa ttcagtgaga tatttatgaa
    20461 aaaccgcggg tcgctcggga taaaaatcca gggtgatgat gagctgcccc gtcaccaagc
    20521 tctggatgtc caactgggcc ttcaatccct gtgtttctac tagatactga acggctttgg
    20581 cctcggtttc caccttttcc cgggttcgca gattcacaac gcggtcctga tcgagttcga
    20641 taataacggc gctcaacagc aattcttttt ccggatgata aaagagccgg atgtcggcga
    20701 cggcaccgac cttgacgcct ttgaaataga ccggagatcc cgtgttcagg ccccggatag
    20761 attcgtcgaa gtacagggca aataatttcc gctcctggaa aaatttaccc gaagagaaga
    20821 taacgacggc ggccgtaaat aagaccaccg ccccgaccac gaaaaggccg atcattttca
    20881 tgctggcctg tttgctcata tcccggctcc ataatatcgt aacatattgt tttttatgcc
    20941 ttctggcttc cgtaccggaa gacgggggcg ccgccgatag ctcccggaac cggtccggtg
    21001 gacggtgcgg agaagtcctc tcgggaagtg atgtttcacc tgcatccctc cccgatcgcg
    21061 ccagggttgc tgacgggcat tgatccgccc ctggtcagaa aattccgcac caccgggtcc
    21121 ttcgcttccg ccagcagggt cttcgggttg ccggcggcga tcatcgttcc agcggcggcg
    21181 tccagaaaga cggaattgtc ggcgatggcg aagatgctgg ccaactcatg ggtcaccacg
    21241 acgatcgtgg catccagact gtcccggagc tggaggatca ggtcgtccag gagacgggaa
    21301 ctgatagggt ccaggccggc ggatggttcg tcgaaaaaaa ggatctccgg atcgagggcc
    21361 atggcccgag ccagccccgc ccgcttcacc atgccgccgc tgatctgggc cggataataa
    21421 tcctcgaatc ccttcagccc caccagggcc agcttcaatg cggcaatctt cctgatctga
    21481 cgggccgaaa gttccgtgta ttcctccagg ggcagggcga tgttttccgc cagggtcagg
    21541 gaactccaca gggcgccgcc ctggtaaagg acgccgaagg tgcgggtaac cttttcccgg
    21601 tcgttttctt ccatctgcca gtagttgcgg ccgtggaaat atatcccccc ccgggccggc
    21661 ttcttgagac cgatgagatg cttgaggagc gtgctttttc cgcagccgct cccccccatg
    21721 atgatgaaga tctcaccccg gcggatggtg aaggtcaggt cccgctggag gacgaagtct
    21781 ccgtaagcca tcgtcagttc ttcaacgaca atatgcgctt tgctgtcgaa gtaatcggtc
    21841 actctatatc cccaggatgt tgcacaacac cgtaatcgtc gccgtggcga tgacgatggc
    21901 gacgatggcc gttacaaccg ccgaggtggt cgccttgcct accgccgcgg cactccgctc
    21961 acattgcaga ccccggaggc agccgatggc ggcgatcagc accccgaaaa cggcgctcat
    22021 gaacagaccg acccacagat cgttgaggcg gatggcgccg attgtctgat ggtaatattc
    22081 cagaaaattg agatccagca tggttacccc gacgatgaaa cctcccagga cgcccatgaa
    22141 atcggcatag agacacagaa gcggcatcat caaaaaaagg gcgatcatcc gcggcaagac
    22201 gaggtagtcc atgggtggga tccccagggt tttgagggcg tcgatctcct cggtggtctg
    22261 catggttccc agttgggcgg cgaaggcggc cccagtcctt cccgccatga tcactccggt
    22321 catgatcgcc cccatctgtc ggaagatgcc gatgcccaca agattggcga tatagatctg
    22381 ggccccgaac atgctcaact ggatggagcc gacgaaggcg aggatgagac ccacgaggac
    22441 gctgatcaaa gagacgatgg gcagggctcg gatcccgcat tcctggatga tgagcagcag
    22501 ttctcggcaa cggaagcggg cccgaccccg gagcagccgt ccgcaagcca tcgtcgcctc
    22561 gccgatgaag gagagggcct ccgcggtgct tatccagaga ccgtaaacgg cttgccccaa
    22621 gcgctccacc aggggcggtc gggcctcttc ctgcctggcc ccggcccgtt ccggcaccgc
    22681 ataggccagc ttcagcaggc ccgctatgcc cggcggcagg ctttcccgct ccacctcgat
    22741 cccatgccgc tgtgcgtaat cgatgatgtc gatgaggaag gtgatcagga cactgttcca
    22801 tccggtcagt ccggaacaat cgaaggcgac gtggaggggc gccggggatt gttccagttg
    22861 attttggacc gcggcaaagg ggggataatt ccccccttcc cgccagttcc cctgtaagga
    22921 aatgaccagc cttcccccct ccggccgtct gaactctatc atttcatcta aaatgcgttc
    22981 ctatgtggtt gacttccttc accaattaat ttcctcccct tcaaggggag gatcaaggtg
    23041 gggatgggtc tatttttcat cccctttgtg acggcatacc gtcatgggtg tttcatgtta
    23101 ttttcaaatc aagggccttc gctaccttga cagccagccg gctttcggga tcgatccggg
    23161 cgtcgctgat catcattatt tccatggtca tggcaacgca tttctcccgt tcttcctcgg
    23221 tcggcaggag cttggccagg gaggcgatgg cctggtcctc gtcgatgagg agcatgaagt
    23281 actggtcccg gatcatttcc cgccattgtt ccttgctgat cttgtcttta gggtatttag
    23341 cctcgagcct gttcgcgatg agactagagc ggtagtcgat catgccgagg tggatcatcg
    23401 cggccatcac gaggcggata agggcttcat tgaaaccgcc ctggtccatt ctgtttttca
    23461 gctcttcgac tttgcggtgg atcgcttctt cccgttccgc gttttcccgt tccaggacct
    23521 gggcctgccg gcccggaaag aggggggcca aacccagcgg tccgtacatc gtcttgaaca
    23581 agttccggta ggcatcgtcc ctcagctccc ggtaggaatc aagaaaatct tcgatggtct
    23641 gggccatgtc ccgttccagc atggcgaaat agttgcccgc gcggatcggc cggcggtttt
    23701 tccgcaccca gtccgcaccg atccggaaag agaccatgcc gggattgaaa cgttccgaaa
    23761 agaggtagta tttcatccgg ctggggttga gccagcgaag tatctccgcc gattgctccg
    23821 tggtcatgag gcgcaccagg ggaccgagaa acatccggta tagttcgtaa ttgtaatcgg
    23881 atacttccgc cacggaggag aaataatcct cgtcccggat ctcatcgctc atggccgtga
    23941 tgtcggcgat ttttcgcatc tcgaagcggg ccacatattc gccgggtatt agttccgcat
    24001 gttccatgaa gaggcttttc ttttcgatga tcatttcgta gagccccggc ggcagcgcgt
    24061 cgatcatctc cagggtattg acgaattcct ggtgctgttt ttttgcgatg gacgtcccca
    24121 cgaagatccc cagatgacct acgtcgggat ggagggtata gacgatggtc tgtccttcgg
    24181 tgagaatcgc ctggtcggag tcgtaaacat cgacgatcca gttcagggcc tgctggggcg
    24241 gggtgatgtt gtcaccctgg gaggcgaaga ccaccacggg acacttgatg ttccgaagat
    24301 cgacccgctg gccgtccggc aggaccacgc cgcccttggc gagccggttg ccgatgaaca
    24361 gattggtcac gatgccgtcg atctcgtcgg cgttcatgag gaaatagccg ctccaccact
    24421 tctcgaagtt gaggtagcgt tccgcctcct ggtcgacgtt ggcgtagagg ttgtattcct
    24481 tccggaaata ggtgttggcg ggattgaggt tctcgaagtt cgtcaccagg taggagccgt
    24541 cgaatttgcc gttccccagg tcgcaggtca acgaggtgag ccacttgcct cccaaaaggc
    24601 cgccggtgta acgcatggga ttcttgttct tgtttccggc ccagtaggac ataggggcgc
    24661 cgttgaggat gatggtgccc atgagatccg gcgccatggc ggccagagag gccacggccc
    24721 agccggcctg gcagtttccc atgacgcagg gccggggcgc ttccgggtgg cggcggatga
    24781 cttcctggag gaagagggcc tccgcctggc ctacatcgac cagcgtctgc cccggttccg
    24841 gcttggggaa aaacatgaca aaataaaccg gatggcccgc tctcatggcg atgcccacct
    24901 gggattcatg cttggaaccc ccgattcccg gcccgtgtcc cgcccgggga tcgacgatga
    24961 cgaagggacg ctttagggga tctatcttca ggccctcctc aggaattatc ctcaggaggg
    25021 cgtaattgac gtgattatcc agggttcgtc cgtcgataat tgtttcgtat tggaattgca
    25081 gcaccggcgg ctcgccttcc tttacgtgcg ccaagaaatt gtccccccgc cggcgcatga
    25141 cgtcccagaa gaggatggat cgctggcatg tatccaccag gtattcccag gcctcgcgcg
    25201 ccgcgacgtt cccctgccag gcctcctcca ggctctggaa gagcgcggcg ggatcgtagg
    25261 cgcccaggca tttcccccag gggacctccg ccggcgtcag gggggccttt atagctccct
    25321 gccacatggc cacgctggat ttaaacagat ctttcaggag gacattgtcc ttccagaggt
    25381 tttcgaatcc cctgaagaac acggcgggat cgaaaccggg aaggagagat tgcggctttt
    25441 cccggtgttc ctcgtttttc cccgttcccg ctgtcccagc cgcagtggtc ggcgccggcg
    25501 cctccctctt cgtttccggc cgtgaggccg acttggcggt ctctcccatc gccgcggttt
    25561 catcaacagg tttggtcgga tcttttgtgt ttgccggcga gaatactgac tccgccgcct
    25621 ctttgatctt tatcgccttt tccttcttcg gcgctgcttt tctggccata gccgcttcct
    25681 ccttggcagg taagtttgac ggcatcttaa aaaaccggtt gcttcgttgc ccttcatcct
    25741 tcgtcacggc gacgtacgtc caggtaattc tcattcctca tgattcgtac gccttgcctg
    25801 cggatccttt tacgatgccg tcccaaatgg gacatttttt gacttactga cgattcagct
    25861 cacaacggcg gaaacgacca ttatcccgtg gtttctactg ttgccgggag ataaatcctt
    25921 tacggggagg gagcggcctt cgtttcggta taaccggcgg ccattttatc cgcctcggtg
    25981 atccagccac cccccatggt cttatagaca ttcacgaggg actggaacag gcttcccttg
    26041 gtctgaacct gggaaagctg gccgttgaac aagctgcgct cggcgtccag aacctctaaa
    26101 taactgctgt atccattgtc gtatcgcagg ccggcaagcc ggcgggtatt cctgagggcc
    26161 atcacctgct cgccttggac ggccagcctt tcccggtatt tccgctggtc caccagggag
    26221 tcgtccacct ctttgaacgc cgtctgcacc gtctgcagat atttaaccag tgcctgctcc
    26281 tggagggcct ccgcctgttt gacctgccct tttatctttc cggcggtgaa gatgggcgcc
    26341 gtcagcgagg cggcgtaatt ccaggtcttg gcgggccccg tcatgagatc gttcagctcg
    26401 gcgctggcga agccgaaggc gccggtgagg gatatggagg ggaagtaaag cgccttggcc
    26461 acggcgatcc gggcgttggc ggcgatcagg ttctgttccg cctggcggac gtcgggacgg
    26521 cgatccagga gatcggaagg gatgccggtc ggcaccgccg gcaataccag ctcatccagg
    26581 gtcctgcctc ggggaatggg accgggattg cgtccgagga gaagattgag agcgttttcc
    26641 tgttgggcga tattcttttc cagttgagga atgaccgccc gggcgtcttc gtattccgac
    26701 cgggactggt tcagctccag ctcggaaatc acgccgcccg cataacgctt ctggaatatc
    26761 tccagggagt ctttgcggct tttcagggtt tccctggcga tgaccaactg ctggtcgtaa
    26821 ttgagcagat tgatgtagct gttggccacc gaggccacca gggtcatgat cacggcccgg
    26881 cgggcctcgt cggtgctgag gagatcggcc cgggccgctt ccgtggcccg tcggagcttg
    26941 ccccagaggt cgagttccca gttggcgttg aggttgccct ggaaggtgta ggaagtaggc
    27001 tgcgtcgtcg ggttccatcc cgttggaccc acttccgtca gacgctgccg gccggcactg
    27061 gctcccagac cgacctgggg gaagagatcc gcccgggtga cgccaagttt tcccatgtat
    27121 tcttccaccc gcgccgtagc gatgcgcaca tccttgtttt cttgcaagga cgtccggatc
    27181 aggtcgttga ggaccgggtc ctgaaattgc ttccaccaag acgtgttggc cagggccagg
    27241 gcgtcttttt cctcgaaacg ccaattatcg ggcgtgtcca cctgcgggcg gctgtaatcc
    27301 ttgcccagca tacagccgct gaggaggagc gccgtgacca ggacgacaaa gagtctcttg
    27361 gtgaaagcga aatatcgatt ggcgggaccg gcagtgagtt tcggcttgac aaccatgttc
    27421 ttatttctcc tccttttccg gcgtcgattc gcctttcttt gccacaggac ccgccagggc
    27481 gccggttttc ttttccagcc gggcgctcaa ggtttcaaaa aactgaaaga acagggggat
    27541 gaagaaaatg gcgataaggg ttgccccgag catcccgccg ataaccccgg tgccgatgga
    27601 gtgacggctg ttggccgagg cccccgtggc gatggccagg gggacgcagc caaggatgaa
    27661 ggcgatggaa gtcatgacga tggggcggag tcggagtcgc gccgcttcca gggccgcatc
    27721 gtagatggac agtccctccg cccgcttcat gacggcaaac tccgtgatga ggatgccgtt
    27781 cttggcggcc agggcaatga gggtcaaaag tccgatctgg aaatagatgt cgttctcgat
    27841 cccccgcatc aggatggaaa ggaccgctcc gaagagggcg aaaggaacgg ccatcaggac
    27901 gccgaagggc agggaccact tctcgtattg ggccgcgagg atcaggaaga ccatgatcag
    27961 accgaagacg aacaccagcc cggaagttcc ccccgcctgt ttggcataaa aggcctcgcc
    28021 gctccaggcg tagctgaaat cctccggcag gacctccttg gccactcgct ccatggcgac
    28081 gatggcctga cccgagctgt agccgggggc ggcgccgccg atgatgttcg ccgccgggaa
    28141 gttgttgaac cggttgacga tatcgggacc ggattcatat ttcatggtga cgaccgactg
    28201 aagggggatc atgttgccgc tggtggaacg tacatagacc tgggtcaggt cgttggggcg
    28261 ggaacgatac tggggttccg cctggacgat aacctgccag aggcggctga acttgttgaa
    28321 ttgacttacg taggccgagc cgaagaggat ctggagggcc tcatagacat cctggacggg
    28381 cacccccagg gtctcggcct tttcccggtc cacatccacc cgcaactgct gggagtaagc
    28441 gttcatggtt gtattgaccc cctgcagttc cggctgtttg tggcaggtgg cgataaacgt
    28501 ctccgccacg ttctggagct ttgccgcatc cccttccccc cggttgagga tccagaactg
    28561 gaaccccccc atattgccca ggccgggaat tgacggggga ttgacgggca tgacaaaccc
    28621 ttccttgatc tgggagaagt tccggaaggc cgcatggagg agcgccgggg ccttgagctc
    28681 ctccttttgt ctttgctcga aatccttgag ggagacgaag atgacgccgg cattcgcctt
    28741 cagggcgcta tcgaccaggc tgtacccgat aaactcgctg acgtcctgaa cggccggatg
    28801 cttcatgaag aactgcgccg cctttgcccc caccgcttcc gtccggtcca ggctggccgt
    28861 atccggcagg aaggtgaccc cgaagaggta gccctggtct tcggagggca caaaactggt
    28921 gggagtgata tagaacatga agccggtgag actagccatg atgacaaaga caatcatcgc
    28981 cgtcttcgtg tgcttgagca tcttggcgac accgccggta tagccggtgg ttatcttctg
    29041 gaagaacgtt tcgaaccatt tgaagaagcc ctttttctct cccgtcttgt gctggaggag
    29101 aagggcggct agggctgggg aaagggtcaa ggcgacgatc ccggagaaaa cgacggagat
    29161 ggcgatggtg atggcaaact gtttgtagag ctgtcccgtc atcccgccga ggaaggcgac
    29221 ggggacgaag acggcgcaga ggaccagaac gatggcgatg accggtcccg tcacttcctc
    29281 catggccttc ttggcggccg cgttcggcgt catatgaaac tcctccatat ttcgctccac
    29341 gttctcgatg acgacgatgg cgtcatcgac aacgatgccg atggcaagga ccatcccgaa
    29401 caacgtcagc atgttgatgg aaaaatcgaa gaggatcatc cctatggcgg cgccgataat
    29461 ggagatggga acggccagga tgggaatgat ggtggcccgg gcgctctgga ggaagatata
    29521 cacgacgagg atgacgagga gacaggcctc aaagaaggta attatgacgt ccttgatgga
    29581 tgcccggacg aactccgtgg aatccatggc gatcttgtag tcgatcccgt cggggaaatt
    29641 tttcttcaac tgttccaggg tcttcttgac ctcgtccgag acgtggaccg cattggcccc
    29701 gggttgctgg taaacggcga gcagcgtcgc cgtcttgccg ttcatcttcg tgcggacgga
    29761 atagtccttg gaacccagga tcgcccgccc cacgtctttc aggcggacga tggccgagcc
    29821 gtccttgctg gcccgcagga tgatgttctc gaactcctgg ggggtggtca ggcgcccctt
    29881 cgtggttacg gaaaaggtct gttccaccgg accgtccgtg ggggactggc cgatcttgcc
    29941 cacggcgaac tgctggttct gctcggagat ggcgttggag acgtcggagg cggtgatgcc
    30001 gagctgggcc atccggtccg gcttcagcca gatccgcatg gcataatcgg gggaccccag
    30061 gatctccgtc cggttggccc cgttgatgcg cttcagggcg tcgaggacat agaggtttgc
    30121 ataattggcc acatagccct ggtcatagcg gtcgtcgggg gaatagacgc cgatgaccat
    30181 gaggaaggag ctcgatttct tctggaccgt caccccctgt ttctggaccg actggggcag
    30241 ttggggcatg gcgatgttca cccggttctg gacatccacc tgggccatgt cggggttcgt
    30301 cccgatgtcg aaaaagacgg tcagggacat cgtccccatt cccgtggaga cggagctcat
    30361 gtagatcatg ttgtcggcgc cgttcacctg gagctcgagg ggggaggcga cattttcggc
    30421 gatgacttcg gcgctggccc cgggataggt ggccgaaacc tggacctggg gcggcgagat
    30481 ctccgggtac tgggcaatgg ggaggttgaa catcgacacg ccgccggcta tgacgatgac
    30541 gatggagatg acggcggcaa agatgggccg atcgatgaag aatttcgaga tcattaggcc
    30601 cccctatttc ttttccggag gggagttctt gcccgccgcc ggcgccgtgg ctgcatcctt
    30661 tgccttatgc tcggggggcg ccgtcgcggt tttcccgccg gaagggtcgg cattacccgc
    30721 tccgtccggc gccaccggct ggccttccga ctttgcgtca ggagctttct tttcaacgat
    30781 cttaaccggc attccttccg ccagcatcat caccccgtcc acgataaccc ggtcgccggc
    30841 tttcaagcca ctggtaatga atatgtcttc gccatgccat tcgccgacga ttacatggcg
    30901 gacctcggcc ttgccttcct tgtccacgca ccagacgaac tgacccttgg ccccttcgaa
    30961 gaccgcccgt cggggggcga cgatggcctt ggggcggacg gcgcccttga ggcgcacccg
    31021 aacaaactgt cccggcttta gaataccttg gggattgtcc atggtggcct tgataagaaa
    31081 ggttcccgtt tccttgctaa aggaaggatc ggcaaagatg agatgaccct tttgggggta
    31141 gatggaccca tcggccagga ccagctccac gatgaatttc tcccccggcg gaaagcggag
    31201 ggtgtctttt ttgatctctt ccagggagtt gagatactga ttctccgaca ggctgaaggt
    31261 aacccacatg ggattcatct tggccacata ggtcaggagg ctgtccgaag cggatatata
    31321 cgttccatcc tgttttttgg cctgactgga gaggccggtc accggggagg tgatgacggt
    31381 atagctgaga ttgatctccg cttcccggac cttgccctgg gcggccagga ctgaggcggc
    31441 ggtggcctgc tcctggcccg tggcgtcgtc caggtccttc ttgctcacgg cgttttgttc
    31501 caccaagggc ttgatccggg ccaggttggc cttggcggtg gcccagcggg cctcctgctg
    31561 ggccagttct cccttggcct gctgcagggt ggcctggaag tttcgggggt cgatgcggaa
    31621 caggacctgc ccggctttca ctagggaacc ttcctcatac acccgcttgt ctaggaatcc
    31681 ttccacgcgg gagcggattt ccaccagatg ggaactctcg gtctgtccca ctacctccca
    31741 cactacggga gtgtccttcg gggcgatcgt aaccacgttc acctcggccg gcggtcgctg
    31801 gacgccccct ttctgatcct ttccacatcc ccacgggatt ccggaaaaca gaagcagaca
    31861 aaataaaaga atcacgattt tagatgaaaa atcacgctcc gggatgaaat tgcgacagtt
    31921 tgccgagttc ataagatacc ccccctatga aaataacgaa agtttaactc attttcaata
    31981 tcctgtcaaa aaaaaacagc cgatagattt tccaaaaagg tccgtgaagg cgaggttttg
    32041 cggcgtcgga tataattata attcattgat ccaggggttg agagaccctg ttgtcttcca
    32101 cccaggattt cagtagggtg aaggctacag ccagcaccac cgggccgatg aatatgccga
    32161 cgatgccgaa ggcgatgagg ccgccgatca ccccggcaaa gacgaggagc agggggagat
    32221 ccgcacctct tttgataagc atggggcgga ggatattgtc gaggctgacg atccctgccg
    32281 tccagacaag gagcagcgaa cccgcgatgg cgttactgct ccagaacagc catacgacgg
    32341 cgggaagcaa gaccaacccc ggtccgagct gggccaggca aagtacgaac attatcgccg
    32401 tgagtgccac ggccgccggg acaccggccg ccgccaggcc gatgccgccc aggagggact
    32461 gtgccagggc cgtaacaacc actcccatgg cgacgccccg gatcgatttt tcaaccagag
    32521 aaacgatctc ttcgccccgc tttcccccca ggcgctcggc gaaacggcgg atgccggtcg
    32581 tcgcgacctc ccccctcgca taaaggatcg tcgagatgat tacggtcagc aggaactgca
    32641 ccagtatctt ccccaagttg ccggcttgat cgatgaacca tttggctgcc tgtcctgaat
    32701 acggtttgat ccgtgcgata attccttccg gtccggccgt cgccagatcc tgccaggtcg
    32761 ccgcaaggtt ggggccaatc aacggtaatt tctgaatcca agacggaggc gggggaatgg
    32821 ccgccgcaca gaaagacgcc gccacttcgt cgatccgacg atagttgtcg gcaatggtag
    32881 ccaaagacac ccataacggc actacgaaga ccagcagcag acccagggtc atcaccagga
    32941 cggcaagacc tcgtttgccc cacagtgttt cctgggcgcg caacatcagc ggccaggtgg
    33001 tgatcacgat tgtggtcgcc cagatgaacg gcggcaggaa cgggtggagg attaagtaaa
    33061 cggcgatgat caagacggcg atcaacagca ccgtaagggt tatatgggtt acgtctttgc
    33121 gcttcgattc tttcattttg ctctggacca tcagaatgga ttgtctctcc gacatatctt
    33181 ttccattttg tcaacaggaa agtcttgtca atttcctatt ttcattgtag attaccatcc
    33241 atagaatcaa tagatggcaa gatatgaaat aaaaatcggt atcagcactg aataccgaaa
    33301 gaatcaaccg ccgctttcga aaaataatgc tggtaaaggt ttgttggatg cccctaaacc
    33361 ccgttgcctg tccggattgc gatctactcc agaacattcc ccatctgccg ccgcaggcca
    33421 aggcccggtg ccgccggtgc ggcaaggttc tggcaatcaa cagaccgagg gccaatgaac
    33481 gcactttggc cctggcgatc ggagcagcga tcgttttcac cctcgccaat ggctttccac
    33541 tcatgggttt gtcggtggcg ggccgggagg tcgatacgac cattatgggc ggcgcctgga
    33601 tgatgtggca ggagggacag cagatgacgg cggcgctcgt cgccatctgt gccgtcattg
    33661 ccccggcgat atatatcagt ttcacgatcg tcgtcctgat cacctcccga tggtatcgtc
    33721 ccgcgccggt atgggtggga gaactgctgc tgtgggcgga actggcccat gaatgggcca
    33781 tggccgaggt gatgatgctc ggtatcctgg ttgccctggt caagatcgct gatctggcca
    33841 ccgtaatcgt gggggtggga atgttcgcca ctggggcact catcgttctc attaccatga
    33901 ttgccgtgaa tttcgatccg gaaaagatct gggcacaggt gcaatgggtc ggttccgtaa
    33961 cgtcggagcc ggtcggcggc ttgcggacag gcggcgatcg ggaggccgaa aaatgatcgc
    34021 cggagagaca tcagccatga gggcgggcct ggcgctgtgc aggcactgcc ggttgttgtc
    34081 caggatcgat acgaccggga gtgttaatta ctgcccccgc tgcggtcacg aactcgcgta
    34141 ccggcgtcat gacgcgatcc agcgtacctg ggccctcatc atcgccgccg ccatctgcta
    34201 tcttcccgcc aacctgctcc ccgtcatgaa atccacgacc ctggtcggag cttccgaaga
    34261 cacgattatc ggcggggtca tcctcctgta tctgacggga tcgtggcacc tggccctgat
    34321 cgttctgatc gccagcgtga tgattcccct ggccaagctc atgtcccttg cctatctgct
    34381 cattaccgtg cagcgccgct cgacgaagag caatcaggaa cgggtccggc tttatcggct
    34441 cgtcgaattc atcggccgct ggtccatgct cgatgtcttc gtcatcgcct tcgtcgtcgc
    34501 cctggtgcag atcaagcccc taatggcagc gcagccccgg gccggtatat tattctttgc
    34561 cgccgtagtg atattgacca tatttgcggc ccaaaccttt gatccccggc tcatctggga
    34621 tttcgccggg aatatggaga agcgaaatga gtgaaaccag tccagcagcc gacatcccgg
    34681 aggcgacgac cgctccacgc aaacgggcgc gtttttctgt cgtctggatc attcccatca
    34741 ttgccgccct ggtgggcatc ggcatcgccg tggagcgatt cctgaacgaa ggtccgacca
    34801 tcaagatctc cttcaaggtg gccgaaggtg tcgaggccgg caagacgttc gtcaagtaca
    34861 aggacgtcaa tatcggccag gtcacggcgg tcagcctttc cgaagattac tcccacgtca
    34921 tggtgacggc caagatcgcc aagagcgcca agaacctcct cgtcaaggac accaagttct
    34981 ggatcatgca tccccggatt accctgagcg gcgtttccgg tattggcacc ctgctttcgg
    35041 gaaattacat cggcattgaa gcgggaaaat ccaaagagaa ggaaaaggtc ttcgtcggcc
    35101 tcgatacccc gcccatcatt accggcgcgg accccggccg gcagtttgtc ctgaaggccg
    35161 aagatctcgg ttccctgggc tacggctccc ctgtatatta ccgccgtctg aacgtgggcc
    35221 aggtcattgc ctatgatctg gcaaaagatg gaaaaaccat ggagatcaaa atcttcgtga
    35281 attctcccta tgacaaatat gtcaccaccg ccacgagatt ctgggaggcc agcggcatcg
    35341 acgtctccat gggagtcaag ggactcctcg tccgcaccca gtccctggct tcggttctcg
    35401 ccggaggcat cgcctttgaa acccctccct actcccaggc cggcgagcag gcggcggaaa
    35461 acgccgtctt taccctcttc gagagccagg aggcggcaac gacgaagcat gaaatgaaag
    35521 acgatccgtt catcctcgtt ttcgatgaat ccatccgggg cctgtccgtc ggcgccccgc
    35581 tcacctacat ggggctcgag atcggggaag tgaagaaaat cggcattcat tacgatcggg
    35641 attcctatac gttcaaaccc tatgccctgg tatcctatta tgaggagcgt ttcttttcgc
    35701 aagtcgatga gaaagacaag gatgccgccg tccgcgatca tcccgccaag gcctggcaag
    35761 aagatcaagg cacccagaag ctcatcgaca aaggaatgcg ggcgcaattg cgcctgggga
    35821 acatcgttac gggacagctt tacatcgccc tggattattt cccccgggcc cccaaggttc
    35881 aggtggactg gacgaaaagc cccccgcgtc tgccggtgat gcccagcggc atggcggaat
    35941 ttcagatgaa ggtcacgggt atcctggata agatcgacaa gatacccttc gacgatatcg
    36001 gcaaggaaac caagacggcc ctgaacggcc tcgatcaaac gctcaaggac gcccgtaagt
    36061 tgctgggacg catcgatgcg gaagtggtgc
//
LOCUS       DAOSMF010000012        34577 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_1646_length_34577_cov_25.950959, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000012 DAOSMF010000000
VERSION     DAOSMF010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 34577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34577
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1646_length_34577_cov_25.950959"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            450..1481
                     /locus_tag="PLT63_02860"
     CDS             450..1481
                     /locus_tag="PLT63_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HOD97692.1"
                     /translation="MKALYLSRICDLKTDRRPLELVETEPPQAGSDEIVIEVGACGVC
                     HTELDEIEGRTAPPFFPVIPGHQVVGRVVEKGDAVTEWRLGDRVGVGWIYSACGECPW
                     CRSDRENLCPEFRATGRDAHGGYGEFMKVRGRFAVAVPETFTDGEAAPLLCAGAIGYR
                     SLRLTGLRDGQLLGLTGFGASAHLVLKLVRYIYPRAAVFVFARSERERSFARDLGADW
                     AGDTEAAPPEPLDAIIDTTPAWRPVVAALENLKCGGRLVINAIRKEGTDRNILMDLDY
                     PRHLWMEKEIKSVANVTRRDISELLALAAAVPLKPEIQEYSLDEANAALLELKERRIR
                     GAKVLRIKI"
     gene            2214..2942
                     /gene="rpiA"
                     /locus_tag="PLT63_02865"
     CDS             2214..2942
                     /gene="rpiA"
                     /locus_tag="PLT63_02865"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF001924.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HOD97693.1"
                     /translation="MQTDITELKKKAAAHAVGLIASGMVVGLGHGSTAIHAVHFLAER
                     LKSGEIKNVLGIPCSLQVEEEALQCGMPLTTFEKHPLMDITIDGADEVDIELNLIKGG
                     GGALLREKIVAQASRRVVIVLDESKCSPFLGKQRHVPVEIVSFGWNTHVFFLESLGAK
                     VRIRRNADKSLYHSDQGNLIADCNFNEILNPRELAAIIKTHAGVVEHGLFLDLATDVV
                     IAGAGGVRHLKRDRPAPAGKGDIE"
     gene            complement(3331..4560)
                     /locus_tag="PLT63_02870"
     CDS             complement(3331..4560)
                     /locus_tag="PLT63_02870"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOD97694.1"
                     /translation="MKAGNPQALFQHLIVLCKEVSEGEYRDMGELFELTKAQLYPPLV
                     AELAESFGMMTVRLEAREYLLEEVITDLKKIQQELAAAKEALARENVSLKHSLKRSLQ
                     PTQILGASAAILYLLKKIDRIAGTPVNVLITGETGTGKELVARTIHDKSARRGRPFIA
                     LNCTAIPESIFESEIFGIEKGVATGVEKRIGKLEQAQGGTIFFDEVGDMPLAAQGKIL
                     RVIEDRQVERIGGRTSIPADVRILAATNRNLKERIAKGIFREDLYYRLNVVNLHIPPL
                     RERPEDISLLLDFFSERGARMMGRTPISFSPEARERLGAYSWPGNVRELENEVERAIA
                     LASGREIGGGDLSENVLGSSPEENLDSLLPPVKRAERLLLEKTLRSVKGNKSEAARLL
                     GLSREGLRRKLLRYELK"
     gene            complement(4557..5273)
                     /locus_tag="PLT63_02875"
     CDS             complement(4557..5273)
                     /locus_tag="PLT63_02875"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HOD97695.1"
                     /translation="MGKRVIVFLIGLVCCGTASVAGTEESLDASLKKNFPQLHYESLQ
                     PSNIKGLYEVETARGIVYYAPDAACLIIGNIFSMEGRNITRQRHNEKILARAKNLPLD
                     KALKVGSGRHTIIEITDPDCPYCRKAAQALSGKDDLTRYIFFYPLSKSSADKVRHILC
                     APDPQEAYKESYRGLLDKKTLALCEKPAVQELMKTHQEQGRRLGVEGTPYFIINGHHI
                     VPGADIPVIEKLLAAPIEKK"
     gene            complement(5320..5760)
                     /locus_tag="PLT63_02880"
     CDS             complement(5320..5760)
                     /locus_tag="PLT63_02880"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD97696.1"
                     /translation="MDTSTGIVVPAQLVYLQKMLDFVTSFTKKAGFDLKRIHEIELCV
                     EEVLVNIFNYAYPATTGDVALVFQPGPEDTLEIRIQDTGIPFNILSVNDPDINAGIDE
                     RQIGGLGIFFVRQLMDRVEYRRDHDRNILSLFVKMPPQRELSGG"
     gene            complement(5776..6108)
                     /locus_tag="PLT63_02885"
     CDS             complement(5776..6108)
                     /locus_tag="PLT63_02885"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOD97697.1"
                     /translation="MEINKKKEKDVLIVSVSGRIDAVTAPDFEKNLMESIGAGEKTFL
                     LAMDHLEYISSAGLRSILAIAKVLKTKEGKLVFAGLQGPVKDVFKISGFGSIFTIFDT
                     EAEALKQL"
     gene            6339..7220
                     /gene="pdxS"
                     /locus_tag="PLT63_02890"
     CDS             6339..7220
                     /gene="pdxS"
                     /locus_tag="PLT63_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HOD97698.1"
                     /translation="MDKKRYELNVQLAQMLKGGVIMDVTNVEQAKIAEDAGAVAVMAL
                     ERVPADIRAQGGVARMSDPAMIVEIKKAVSIPVMAKARIGHFVEAQILEALKIDYIDE
                     SEVLTPADEECHIDKTAFPIPFVCGARNLGEALRRIAEGAAMIRTKGEAGTGNVVEAV
                     RHMRTMNREIRQLARLPREEVTGFAKANGIPYELALQVRELGRLPVVNFAAGGIATPA
                     DAALMMQLGCDGIFVGSGIFKSADPARRARAIVKATAYFQDPQKILEASMDLGEAMPG
                     LEIGSIPENQLLAGRGW"
     gene            7236..7889
                     /gene="pdxT"
                     /locus_tag="PLT63_02895"
     CDS             7236..7889
                     /gene="pdxT"
                     /locus_tag="PLT63_02895"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HOD97699.1"
                     /translation="MIGVLDLQGDVVEHLDHLDRLGVSARRVKDRSDLANLQGLIIPG
                     GESTCLARLLVIFGLDAAIRSAYRRGMKIWGTCAGAILLASRVMGGKAHLGLIDMEIE
                     RNGFGSQLDSFTGRAVIPKVSKVPVPLIFIRAPKIRSVGPGVEILWQAEDYIAAVETR
                     EGLATVFHPELTNNLAFHSYFARKCGLDIDTRPASPSEEDSSWTITSWTRHMRIAPG"
     gene            complement(8001..8522)
                     /locus_tag="PLT63_02900"
     CDS             complement(8001..8522)
                     /locus_tag="PLT63_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97700.1"
                     /translation="MNYNNIKKIALCFIFTALITGCAGSGNVPDWTYAAFNMLEDFKQ
                     AALEGRTTIAELHFQRAVEEIKKSGDLVLLGRAYLNRYAVQTALLESFDDGKFQKIQS
                     VHPDRENAAFLSFLKGNFDRTDSKLLPSPYAAAQNDLLEGNAKHDVHESGSSPCPCRS
                     VHVWIPALQGMRW"
     gene            complement(8681..9322)
                     /locus_tag="PLT63_02905"
     CDS             complement(8681..9322)
                     /locus_tag="PLT63_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97701.1"
                     /translation="MAYRHPVGLVSREMPLISNLSVLENIALIRQYHSRLSRRRAEEM
                     AREYLRRPQLEDLVGRLNPTLREEQRFCVMVLRAVMLEDSILVLDRPFEMMPALGDRK
                     SVQAIHASLRNTRQITDQVNILLKKTDEDIYGKDGILPLVTKALRELANNLAKIGATL
                     DNITKITSDAAGSTKDLKLLRSDIDATVNSLNRLLDELNRKMPFKSEPKIKLP"
     gene            complement(9376..9636)
                     /locus_tag="PLT63_02910"
     CDS             complement(9376..9636)
                     /locus_tag="PLT63_02910"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOD97702.1"
                     /translation="MSARDYIAALLESANFFDLIAMVVKSAVFGFFIVMIPIRHGIRA
                     TNELTSISAAVLIGMVNVFIAIVIVIIEVLSLVITPMITKLI"
     gene            complement(10010..10180)
                     /locus_tag="PLT63_02915"
     CDS             complement(10010..10180)
                     /locus_tag="PLT63_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97703.1"
                     /translation="MLNVLDSIGEKGLQAARSLRDAMSFSYLIFARLFDRRAYNSAVS
                     MVLVNQIYFKAV"
     gene            complement(10222..10833)
                     /locus_tag="PLT63_02920"
     CDS             complement(10222..10833)
                     /locus_tag="PLT63_02920"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOD97704.1"
                     /translation="MGPAAADYFSNLAGRLEREVNTGATPIQILDVGAGAGTMSLLLA
                     RTLPSPAITGIDYSPGQVRAAQEMKKKAGLDNCRFQLGNAMDIPFAAESFDLVISVNS
                     IKHWPDPVRGLTEIRRVLRPRSRAFIAEVDRNTSPAGLREFADKYCAWYVWPPVMHWC
                     LRNVVFGNSYTAGEATNFACRAGFSAVSLQEVPGPFFLIVLTK"
     gene            11198..12016
                     /locus_tag="PLT63_02925"
     CDS             11198..12016
                     /locus_tag="PLT63_02925"
                     /inference="COORDINATES: protein motif:HMM:NF013706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOD97705.1"
                     /translation="MKIYHCLADDGRRYYGQLHKEDQGLLEVWEGNLFAPWTPTETVV
                     KINKLLPPLLLPPNIFALGLSYRRHADETGIEVIPEPLVFMKASTSVIGPEDTILLPD
                     AGPAKVDYEGELAVVIGRRGKNIPPPEAMDYVLGYTCANDVSARDWQFERQQGQWVRG
                     KSFDSFCPLGPCLVTRDELPDPNKLAVRTVLNGQIVQDASTNGMIYDIATIVSNLSKS
                     LTLLPGTVILTGTPEGVGFTRVPPLFLRDGDRISVTIEGIGTLANPVRAERIDA"
     gene            12209..13681
                     /locus_tag="PLT63_02930"
     CDS             12209..13681
                     /locus_tag="PLT63_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HOD97706.1"
                     /translation="MESKNRKTFFMFLLACLIGFFLVSVVEVLRSSFLVPTGTDVMPA
                     SAITPGDGSRAPGSFADLAEKVKPAVVNISTTKVMKGGGYRSPFGGTPFDRYFGDDFF
                     ERFFGDSPRREFKQQSLGSGFIISSDGYIFTNNHVVEQADKILVKLSDGREYEAKVVG
                     KDAKTDIALIKIKAGDSLPVAEIGDSEKLRVGDWVIAIGNPFGLEQTVTAGIVSAKGR
                     VIGAGPYDNFIQTDASINPGNSGGPLFNMEGKVIGINTAIVAQGQGIGFAIPIAMANN
                     VLPDLKAKGKVTRGWMGVTVQDITEDMARSLQLKDRRGAIITEVFKGDPADLAGLRPG
                     DIVTAINGKKIKDTHELLLLISSFHVGDKVSISVLRDGRAMNFQVTVAERRDRPETAR
                     SSRGGYEHYGLAVGDITPEIARYLGIPKKTGVIVMRVQPGSPADEVGIQPQDVILQVN
                     KVKISSVKDYLREVNKKAASGSVMLLIKRGQATFFVALRK"
     gene            13871..14593
                     /locus_tag="PLT63_02935"
     CDS             13871..14593
                     /locus_tag="PLT63_02935"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HOD97707.1"
                     /translation="MDKARWLDDTYRILDDHFGNLHWWPGDSPLEVIVGAILTQNTAW
                     KNVEKAIAALRRHALLSVAALVEIPECELAGLIRPSGYYNVKARRLKAFFIFLRDEFR
                     GSLEVMFSEDVPMLRQKLLGIKGIGAETADSILLYAGGKPVFVVDAYTRRILSRHGIV
                     ATRSSYGEIQRLFMDRLPADAALFNQYHALIVYTGKNYCRKQPRCQECPLYPFSVPQH
                     IDMDMNGRHKKPRTATALGEVV"
     gene            14597..16054
                     /locus_tag="PLT63_02940"
     CDS             14597..16054
                     /locus_tag="PLT63_02940"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HOD97708.1"
                     /translation="MKTTLGVMEMLKKIDDCRWEVPQSGGMRVPGIIYSSERLLRDMG
                     SDESQKQVANVAHLPGIVNYSLAMPDVHWGYGFPIGGVAAFDVENGIISPGGVGYDIN
                     CGCRLMTTRLAYEEIRGSLRELVTALFQNIPSGVGSKGDLKLSVKEEKKVLEEGAAWA
                     VRQGYGTPADLETTEDGGRMAGADPEQVSDRALERGREQLGTLGSGNHFLEIEVVEEI
                     FDPQAAAAFGLELHQVVVMIHSGSRGLGYQVCDDYLSRMVKHVNELGFSLPDRQLACS
                     FLRDRRGQDYYAAMAAAANYAWANRQLLMHWTRETFEKTLKKSPREVGMRLLYDVCHN
                     IAKLETFSIGGRERQLCVHRKGATRALPPGHPALPPAYSGVGQPVLIPGNMGAGSYVL
                     AGMQRAFEETFASACHGAGRVMSRSQAIKASKGRAIVREMAELGVTVMASGRGTLSEE
                     IPGAYKNLEEVVAVVHRAGLARNVARLRAVGCIKG"
     gene            16085..16252
                     /locus_tag="PLT63_02945"
     CDS             16085..16252
                     /locus_tag="PLT63_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97709.1"
                     /translation="MNFGTASWKADQCDIHIPSQALEIGLINRVVADDDYQEGLRAFF
                     DKRGEVHREMM"
     gene            complement(16361..16600)
                     /locus_tag="PLT63_02950"
     CDS             complement(16361..16600)
                     /locus_tag="PLT63_02950"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HOD97710.1"
                     /translation="MAARQETRKALYHKYNPVMIVSAYGFVIAIASFLFLYLGFKIDQ
                     YFGTAPNFMFGMFFLALFLSIGRLYQEAWLKRKEV"
     gene            complement(16639..16914)
                     /locus_tag="PLT63_02955"
     CDS             complement(16639..16914)
                     /locus_tag="PLT63_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97711.1"
                     /translation="MKKTLGTLVAAIVVVLCTGTFALAEYAAAGVANFPYFQLGCLIV
                     GGMVIISLKHKYEKMYTGEVAGVFALYTVLIALFTNPVIEGIKNLVG"
     gene            complement(17205..18200)
                     /locus_tag="PLT63_02960"
     CDS             complement(17205..18200)
                     /locus_tag="PLT63_02960"
                     /inference="COORDINATES: protein motif:HMM:NF021230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97712.1"
                     /translation="MSTKIQTAAAILLSALILFSFSFTVRAEEISRSGKYMSTSLETT
                     GAPVSRLSAATPPVATSAAADEEERPTGDFTVAAISQYIWRGYEMSRHSIVVQPATTI
                     GYKGFSAGLWGNLDMRPYFAGATDTNYASSWNETDFTISYSKKLGIFNLGAGYIYYSL
                     GALNKDAPDRADAQEIFASVSLNTLLAPTMTAYKEMDHYRNWYFLLGISHTHDFTKRV
                     SLKIAATASYLLSTYADAALFNAGEGYGGYPRFNSDAAAMDDKFNNFHDGTVTMSLPV
                     KATGQITVTPTISYVFPLSDDARYEMKGTGLKGTASAVDRESSYLYGGLSVCFSF"
     gene            complement(18393..18734)
                     /locus_tag="PLT63_02965"
     CDS             complement(18393..18734)
                     /locus_tag="PLT63_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOD97713.1"
                     /translation="MKYIIAIIQPHKLDEVMAALEKVEIPLVTVSNVLGRGRQKGVTE
                     VYRGAKEAGSLLKKVKLEIAVNEDYVAPALAAITDSAHTGEVGDGKIFILDLYEVLRI
                     RTGERGGVAIG"
     gene            complement(18808..20040)
                     /locus_tag="PLT63_02970"
     CDS             complement(18808..20040)
                     /locus_tag="PLT63_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HOD97714.1"
                     /translation="MNTGDTAWVMLSTILVFAMTIPGLAFFYGGLVRRKNVLSVLMQC
                     FIIMCVISLQWVLFGYSLSFGADTGQGIIGGLEWAGLNHVGGQPNGDYAATIPHGVFM
                     IFQCMFAVITPALMIGAFAERMKFSAFLVFTILWTTLVYDLLAHWVWGTGGWMRNLGG
                     LDFAGGIVVHASSGVSALVLCILLGKRLGYQKTPFRPHNLPFTVLGGALLWFGWFGFN
                     AGSALGANELAANAFVVTHIGTAAAGLTWALIEWQRHGKPTILGTVTGAVAGLVAITP
                     ACGFVNPMNAIFIGIVVAFVCYIAVTVIKTRLGYDDALDAFGVHGVGGIWGTIAVGLF
                     AEKAVNASGADGLFFGGAQLWPQVVMAVAAPLYAVIMTIIIYKIVDALMCVRVKEREE
                     NIGLDLTQQSEAAYTVIE"
     gene            complement(20366..20881)
                     /locus_tag="PLT63_02975"
     CDS             complement(20366..20881)
                     /locus_tag="PLT63_02975"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOD97715.1"
                     /translation="MDRRHLRRGRGIGAGGGTIRRIAIPDFKKYLDNIRAGIKGLNPF
                     MDFIGIEPLEIKEGFARFRLPVRPEYLQGAGRLQGGLMVALADEAIAHAMVTTLDDRE
                     GLTTIELKSNFLAAVSSGAVTATATVFKKGQSLIIGDCLLTDDRGRNVCRVSATFLLL
                     QERQRNNNKLD"
     gene            complement(20975..21385)
                     /gene="rnk"
                     /locus_tag="PLT63_02980"
     CDS             complement(20975..21385)
                     /gene="rnk"
                     /locus_tag="PLT63_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HOD97716.1"
                     /translation="MTTEKRNIFITEKDMERLEPMVAGAGNHPNIRRLREELERAVIV
                     PSAEIPADVVTMNSRVVFRELETGEETEATLVYPSQADVNQRRISILAPVGAALLGLS
                     AGDEIQWPLPSGKKRTYKIISILYQPEAAGNYDL"
     gene            21702..22859
                     /locus_tag="PLT63_02985"
     CDS             21702..22859
                     /locus_tag="PLT63_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97717.1"
                     /translation="MAMAEGEMKYGALVRTGMLMGMRKGWSGFVWMMKIVLPISLLTA
                     LLEWSGWLRQLDFLLTPAMSLLHLPAAAALPLIIGMLANIYGGIAAMVLLPLTEGQMT
                     LIGVFLLMAHNLIQEGVIQGQSGIHPVKATLFRIVTAALTVMIIGPWVAGVPAVTPPP
                     TLRAANTVAAPAGDQGARCEPMVVPTSPPPAASVPAGATNTKIPATVPPVPVSNAPGS
                     PATPSTGIAAADTSPALPLTDMLRQWFWTTAVLCGKIFLIIMGIMTLLETMKFLDWIK
                     YIVRLFSPLLRVMGLDAQMGLLWMTAIVFGLAYGGAVIVEEAKAEHLSKDELEILHLS
                     IGINHSMVEDPPLFISLGLSPFWLYIPRLIVAVVTVHLIRLWRRVRHPQAR"
     gene            23257..24552
                     /locus_tag="PLT63_02990"
     CDS             23257..24552
                     /locus_tag="PLT63_02990"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOD97718.1"
                     /translation="MRFEEFALNAKLLAEVQSMGYTMPTPIQAKSIPPIMAGRDVMGL
                     AQTGTGKTAAFVLPILQRLMSGPHGPVRALIIAPTRELSEQTHKVIGLLGRKTRLKSA
                     SIYGGVNINGQIKLLRDRIDIISGCPGRLLDHIDRGTLDLSKIEVLVLDEADHMFDMG
                     FLPDIRRIVKRLPGRRQTLLFSATMPADIQKLADDLLQNPLHIKIGHNAPVESVSHHF
                     YPVPMDVKTALLREILDRTHRDSVLVFTRTKSRAKSVAGQLGKSGYKAISLHGNLTQQ
                     KRQQAIDGFRAGRYDIMVATDIAARGIDVAGISHVINYDMPATLDAYTHRIGRTGRAS
                     GTGEAFTFVTGQDMELAQALKRSLGDKLTYHRVDGLTTMVMPDKRDAADIRPDRTGAG
                     NPPDNRKGGRSFSPRGHRSRCGKSQPRTLPPAGQRSWGH"
     gene            complement(24621..24887)
                     /locus_tag="PLT63_02995"
     CDS             complement(24621..24887)
                     /locus_tag="PLT63_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97719.1"
                     /translation="MPTASFGDIRDDLAIRTLTDHECRLEVAVALEMSDGGKELLVPG
                     AQHDPAAAPEMVHPSFFILDPYPERQAVKPNKMVEKRMKPSGSK"
     gene            24825..25277
                     /locus_tag="PLT63_03000"
     CDS             24825..25277
                     /locus_tag="PLT63_03000"
                     /inference="COORDINATES: protein motif:HMM:NF015961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOD97720.1"
                     /translation="MISKSSDGEIIADVAERSGWHAVRGSSSRGGKEALRKMIDHLSL
                     TGLAGHILDGPRGPAGIVKAGVIELAHAADAVITPFYTSADRAWYFNSWDNFILPKPF
                     AKVLISFGNMIKLLPVEDAIAFECQRLELEKTMIPELRVKPAGKSAHM"
     gene            25454..26761
                     /locus_tag="PLT63_03005"
     CDS             25454..26761
                     /locus_tag="PLT63_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOD97721.1"
                     /translation="MIYNEEFETLPREALEALQLKRLQQVVQRVYHTVGFYKRTFDAA
                     GATPDDIRTISDLQRLPFTTKQDLRDNYPFGLFAVPMSSVVRLHASSGTTGRSTVVGY
                     THRDIETWSELVARCFVAAGLTKNDIIHNAYGYGLFTGGLGAHYGAEKLGASVIPMSG
                     GNTKRQLMILQDFGPTAICCTPSYALNLAEQGGAMGVDMRALKLRVGIFGAEPWSDKM
                     RDEIENVMDITALNIYGLSEIMGPGVAMECQEGREGMHVFEDHFLVETIDPATGKVLP
                     PGETGELVFTTLTKEAFPLIRYRTRDISRLITAPCRCGRTHFRMDRVEGRSDDMLIVR
                     GVNVFPSQIEAVLVGIQGVEPHYQLIVDREGTLDTLEVQVEVSDALFSNADEVRVLQK
                     IERRISKDLKDYLGITAKIKLVEPKSIQRSEGKASRVIDKRQI"
     gene            26778..27209
                     /locus_tag="PLT63_03010"
     CDS             26778..27209
                     /locus_tag="PLT63_03010"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOD97722.1"
                     /translation="MKVEQVSVFLENKPGSLEEVTRILKEANINIRTLSLADTTDFGI
                     LRLIVNNVDTTSRVLRDNGFRVSRTTVVAVEVPDRPGGLHSILAVLKNDNILVEYLYA
                     FVEKSGENAVIIFRFDDSDAAIEVLRKNNFNVISGEKLYSF"
     gene            27266..27928
                     /locus_tag="PLT63_03015"
     CDS             27266..27928
                     /locus_tag="PLT63_03015"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HOD97723.1"
                     /translation="MRSAESLVEEQLGKWRLAGTERKKEKAKVGPVITVSRESGCGGT
                     KIVQKLAKELGFDLMGGKIIQMVAESVEMSEKVICSLDEKEVTKRDDWLNSLFETRHL
                     WPDQYLHHLTKVIGTIGRHGNAVILGRGANYILPQESTFRVRIIGPFDLRIKNATRTT
                     EDEARRYLIKVDADREAFIRKYFNADVKDPAHYDLVINMRVVSIDGAVKAVIAAFNSW
                     KR"
     gene            27958..29286
                     /locus_tag="PLT63_03020"
     CDS             27958..29286
                     /locus_tag="PLT63_03020"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOD97724.1"
                     /translation="MILNIRRKLFLGYLAMALLTIVASTYAVGHLRSLNNLAEKMIHQ
                     DARLLDLEKKMMDALLAWEGVEKRYLILKDPSLADIFWTRSEDFQSQIEKLKAEALPE
                     QVQTITALATLAKQYAEIFVRETVLVDEDRLDEAAALSSHEGRRVMEKMGLALRALQV
                     QTEKNIDSRMKAINIKALEASHMTIALAVASLLIGMILAAAITFNISRRLRKLEVATG
                     DVAEGNFDREIDIRGEDEICSLAAAFNTMTKRLKVLEAMNIDASPLTGLPGNLAIEQE
                     IRRRLELKKPFSLCHLDLDNFKPFADHYGYAWGSEVIKEVAVILDEGKRIRGEAGDFI
                     GHIGGDDFILITEPARAEGLCGFIVAEFDERVQRFYSEEDRRRGFILAKDRQGRLQEF
                     PLITITISIVSDDGSQYHNPLEMAKMAAEVKNYGKSLAGSKYVHREDMPG"
     gene            29315..29878
                     /locus_tag="PLT63_03025"
     CDS             29315..29878
                     /locus_tag="PLT63_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97725.1"
                     /translation="MKIIDITEQSLYRRLMPLLLAAVVTVLAACAAKGPPPPPGSLAA
                     RFQSDTALFQEGYERLRGVDRQKDEAIARQAFVLLMEQYPKSKWRGYAELYLRLLNEE
                     QRLQKELQDLHNRVQADRQGAAVREAGLEKERQELRSLLEQERADAARLQRENQSLRH
                     ENDRLQNNIDSLKRLEIELDRRDKLYR"
     gene            complement(29869..30369)
                     /locus_tag="PLT63_03030"
     CDS             complement(29869..30369)
                     /locus_tag="PLT63_03030"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOD97726.1"
                     /translation="MPVVYTCRIFKVLEEEVTLPNGHRSRLSWIDHNPCIAVVPRDET
                     GNFLLIRQYRHATGGYLLEIPAGNIDRGETPELCVQRELREETGYRASRITKLFEGYL
                     LPGYGNEYMHFYLAEDLEAAPLPPDADEDIAIVRVSSAEARELLVGGSIRDVKTALGL
                     SLVLQR"
     gene            complement(30390..30956)
                     /gene="trxA"
                     /locus_tag="PLT63_03035"
     CDS             complement(30390..30956)
                     /gene="trxA"
                     /locus_tag="PLT63_03035"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOD97727.1"
                     /translation="MPGSDEQIIVRCLNCGTKNRIPRERMQDRPTCGKCRAPLDDMII
                     RCLSCGTKNRMPETRLNERPLCGRCGVPLVVGGDTGQPFDITDAAFTREVLTAPGTVI
                     VDCWAPWCAPCRTVAPILEELAAKYAGGVRIAKLNVDENPVTAAQFDIRNIPTLLFFR
                     DGKLLSRAVGALPKEEIERRLLAVIKPN"
     gene            complement(30965..31453)
                     /locus_tag="PLT63_03040"
     CDS             complement(30965..31453)
                     /locus_tag="PLT63_03040"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HOD97728.1"
                     /translation="MSKEKFPMTPAMRELKSHEAVFRLLAYKYVDRGGTRISARELGV
                     DEHKVIKTLVMEDENGRPLIVLMHGDRDVSTKSLARILGVKAVSPCDPKVAERHTGYK
                     VGGTSPFGTRKRLPIYIEETILSLPLILINAGHRGILAEMNPAELNRILHPIPVQAAI
                     ER"
     gene            31611..32969
                     /gene="trkA"
                     /locus_tag="PLT63_03045"
     CDS             31611..32969
                     /gene="trkA"
                     /locus_tag="PLT63_03045"
                     /inference="COORDINATES: protein motif:HMM:NF007031.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HOD97729.1"
                     /translation="MRVIIIGAGEVGYHIAKKLSEENQEVVLIDQDPAKIRRINENLD
                     VQSFLGSGTSPQALRAVGIEEAELLVAATDSDEVNLIACLLARNLNRHMVKVARVRNR
                     EYLQEQSLFSKDLLGVDLLINTRDMMVETILGLMEVPGASEVIDFVGGKVKMVSFLIS
                     GDSPLAGKKLSDLKAYEGNLLVGAIVRGHKIVIPSGRDTIQPHDLVYFVMKKTELEGS
                     LAQFDMANRPMKNIIIVGAGETGMAIAKALDERRLNVKIIDQDGERCKKLAGILKRVI
                     VIRGDGADRDLLREENAGEMDLIIAVTGDEESNVLISLLAKELGGRRTITRVSKLSYM
                     SIVSAVGLDTVINPRMAAVRAILQYIRRGRILSVAPLREEQAEAIEAEAMETSDIVNT
                     PLCRVNFPPGAILGAIVRGEEIIIPQGDTIVLPHDRIIIFALQQVLPKLEELLTVKLE
                     YF"
     gene            32969..34420
                     /locus_tag="PLT63_03050"
     CDS             32969..34420
                     /locus_tag="PLT63_03050"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015379 - potassium:chloride symporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HOD97730.1"
                     /translation="MNFRLIAYLVAVLLFCIGLSMTAPLGVALYYGDGSAPALLGSLL
                     LTCGAGGGVFFRTRGQEDFYLSHRDGVAIVTLGWLAAGLAATLPYLLSGAIPDFTNAY
                     FESISGLTTTGSSIFKDVEVLPQGILFWRAQTQWLGGMGIIVLSIAILPFLGIGGMQL
                     YKAETPSPVVDKLTPRISDTAQALWKIYIVLTIIQIFLLMSGGMDWFDSICHTFATMP
                     TGGFSPKNASVGHYQSVYIDFVIVLFMFVAGMNFSLHYKLVKGNVRQFFRDPEFRSYL
                     FITAAIILVITIDLYGAAIYKGIFDACRYAAFQVVSIMTTTGFVTADFEQWPVLSQQL
                     LLLCMFLGSMAGSTGGGIKIMRIVLLLKHCYLEIFRIIHPHATTVVKLGEIPVPQTIM
                     RSIWGFFLLYVGIYIVATVAMAALGLDMISSISAVATCLGNVGPGLGSVGPMDNFSGV
                     PVLGKWLLIFCMLLGRLEIYTIIVLLMPGFWRK"
BASE COUNT         7604 a         9509 c         9993 g         7471 t
ORIGIN      
        1 cgatgctgaa ccactgctgg aggaggaggc ctttcataag gaaatatggc acataataaa
       61 ttaaatgaca agcaattaca atatgttaac tttgaacaac cactttttcg gcgagaatat
      121 tgaggaacaa cgaataaaaa aattgccgag ttgtgcaaag cgtctcgaat tgtcatgttt
      181 gtccaacacc cacgaaagat ccaaaaagtt tatagggaga tcgtgcagat gtattttctg
      241 attaagaaga tcgttaccgc tcgatgtatt ggcgaacgat agctgcagat gcactgcggc
      301 aactgccggc atgatagctt ggcggccgga ggacgtcttt cccgtatagc gatccacgtc
      361 cctcgatgcg cattatcatg cagtaacttg agtgaatata gtcagatgat caggaacggt
      421 aaaaacgcgt gcaaggaagg ggaaaaggta tgaaggccct ttatttgtca aggatctgcg
      481 atctgaaaac ggaccggcgg cctctggaac tggtggaaac ggagccgccg caagcggggt
      541 ctgatgaaat tgtcattgaa gtcggcgcct gcggggtttg ccataccgaa ctggatgaaa
      601 tcgaaggacg gacggcaccg ccctttttcc ccgtgattcc cggtcatcag gttgtgggcc
      661 gggtggtgga gaaaggcgac gccgtgacgg aatggcggct tggagaccgc gttggggtgg
      721 gctggatcta ttcggcgtgc ggagaatgcc cctggtgccg ctcggatcgg gagaacctct
      781 gtccggagtt ccgggccacc ggccgggacg cccacggagg ctacggggag ttcatgaagg
      841 tccgggggcg gtttgcggtg gccgttccgg agacctttac cgacggagag gccgcgcccc
      901 tcctgtgtgc cggggccatc ggctaccgtt ccctgcgcct gacgggcctg cgggacggtc
      961 aattacttgg tctgactggt ttcggggcct ccgcccacct ggttttgaag ctggtgcgct
     1021 atatataccc gcgagcggcg gtttttgtct tcgcccggag cgagagagaa cgcagctttg
     1081 cccgggactt gggggcggac tgggcgggtg acacggaagc ggcgcctccg gaaccgctcg
     1141 acgccatcat cgataccacg ccggcctggc gccctgtcgt cgccgcgttg gaaaatctca
     1201 aatgcggggg gcgcctggtc atcaatgcca tcagaaaaga gggaacggac cggaacatcc
     1261 tcatggatct ggactatccc cgccatctct ggatggaaaa ggaaatcaag agcgtggcca
     1321 acgtaacgcg acgggatata agcgaattac tcgcactggc cgccgccgtg cctctcaagc
     1381 ccgaaattca ggaatactcc cttgacgagg ccaacgcggc cctgctggaa ctcaaggaac
     1441 ggcggatccg gggcgccaag gttttgagaa tcaagatatg aggtaacatg caggccttat
     1501 ttaccggcgg atgcggtgct tccgggatga aatgggcaaa tacatgttta cgcaagaggc
     1561 acagggtagg caataatcag cacccgtttg ccctctgact gcctcccgcc aacaggaaga
     1621 ttctctttgt aggcatgtaa cgctggcagc tttatccgag ccggacttga accggctccg
     1681 taatcgccgt aagggaaaaa gaactgactc cggttaggaa tcggcgcggc gaactccctg
     1741 ccctgtggac gatgaaaccc atcccttccg tcagtttcac gactcccttc gggagggatg
     1801 ggcattacat cggcagaaat aattcggcgt gaaagtatgt tgacaaggaa aggcaattaa
     1861 aagtaaaatt ccacgcctag ggccgatagc tcccagatga tcaatccttc tcgtcaaaca
     1921 atgtccctcg aagatgttaa catgctccaa gtgatatata tttaacgata atcaggaaat
     1981 gtttcatgtg aaacagcgag ccttcaatct cttgacctta gcgggcgctg cggtgaatga
     2041 aaaatgccct gaaatcagcg aagctgatta ctgttcacaa atcagcgaag ctgattaatg
     2101 ttcacaaatc agcgaagctg attaatgttc atactggccc tcttcccaca agaagaccca
     2161 tggtgaaaga agaaatttca acataacatc tcataatcga aggagggatt catatgcaaa
     2221 cggacataac ggagctgaaa aagaaggccg ccgcccatgc tgtgggactg atcgcctcgg
     2281 gcatggtggt tggacttggg cacggcagca cggccattca tgccgtccat ttcctggccg
     2341 aacggttgaa atcgggagag ataaagaatg tccttggcat cccctgctcc ctgcaagtgg
     2401 aggaggaggc cctgcaatgc gggatgccct tgaccacctt tgaaaaacac ccgttgatgg
     2461 acattaccat cgatggcgcc gatgaggtgg atatcgagtt gaatctcatc aaaggcggcg
     2521 gcggggcgct actccgggaa aagatcgtcg cccaggccag tcgccgcgtg gtcattgtcc
     2581 tcgatgagag caagtgttct cctttcctgg gcaaacaacg ccatgtgccc gtggagatcg
     2641 tctcttttgg ctggaacacg catgtgttct ttctggaatc cctcggcgcc aaagtacgga
     2701 tccgacgcaa tgccgacaag agcctctacc atagcgatca ggggaatctc attgccgact
     2761 gcaatttcaa cgaaattctc aatccccgtg aactggcggc aataatcaag acccatgccg
     2821 gggtggtgga acacggactt tttctcgatc tggcaacgga tgtcgtcatt gccggggctg
     2881 ggggcgtccg tcacctgaag agggatcgac cggcaccggc gggcaagggt gatattgaat
     2941 aaaagcaccg gatctttcgc cctggcgtaa agataatctt attagtatga aaatgcatcc
     3001 gtgtgatgtc tcctaatcat ttttcatcgt tcgtgggagt tgaataaaca tgacaattca
     3061 ttccattagc agcggtatag gtatttgtcc gatccgtggc aatgacctcc ggcggcagtg
     3121 gcaatcggga gagattggta ggacggagcc gaagttgtct tgagggggaa ttaggaatat
     3181 ctgttgtcgg tgtctatact ccgctgattc cgatggtcgg aatgcgggat gcgtgttgtc
     3241 agccgccttc ctaaggctat ggctggctgg agggctcatg ttagctaacg cttgaagccc
     3301 agagatatcc ctggctcccg ccagtgggtg tcatttcagt tcgtagcgga gaagcttgcg
     3361 gcgcagtccc tctcggctga gacccaggag gcgtgcggcc tcggacttgt tgcccttgac
     3421 gctccgcaac gttttttcaa ggagaaggcg ttccgccctt ttcaccggcg gcaggagact
     3481 gtcgagattc tcttccgggc tggatccgag gacgttttcc gaaaggtcgc cgccccctat
     3541 ttcgcggccg gaggcgaggg caatggcccg ttccacctcg ttttccagct cgcggacatt
     3601 gcccggccag gaataggcgc caagtcgctc cctggcttcc ggggaaaaag atatcggtgt
     3661 ccggcccatc attcttgccc cccgctccga gaagaagtcg aggaggaggg aaatgtcttc
     3721 cggtcgttcc cgcaggggcg ggatatggag gttgacgaca ttcagccggt aataaagatc
     3781 ttcccggaaa atacctttgg cgatccgctc cttgagattc ctgttggtgg ccgccaggat
     3841 gcggacatcc gcggggatgg aggtcctgcc gccgatgcgc tccacctgcc ggtcttcgat
     3901 gacccggagg atctttccct gggccgccag gggcatgtcg cccacctcgt cgaaaaagat
     3961 ggtcccgccc tgggcctgtt ccaatttccc gattcgtttc tccaccccgg tggcgacacc
     4021 cttttcaatg ccgaagatct cgctctcgaa gatcgactcc gggatggccg tgcagttcag
     4081 ggcgataaag ggccggccgc gccgggcgct tttatcgtgg atggttcggg caacgagttc
     4141 cttacccgtt cccgtttcgc cggtgatgag gacattcacc ggcgtgccgg cgatccgatc
     4201 aatcttcttc agaagataca ggatggcggc actggcgccg agaatctgcg tgggttgaag
     4261 ggaccgcttg agggaatgct tcaaactgac attttccctg gccagagcct ctttggctgc
     4321 cgccagttcc tgctggatct ttttcaggtc ggtaataacc tcttccagaa gatactccct
     4381 cgcctccagc cttacggtca tcatcccgaa agattctgcg agttcagcaa ccaggggcgg
     4441 atacagttgc gctttggtaa gttcgaatag ttctcccata tcccggtatt ccccttccga
     4501 cacctctttg caaaggacta tcaggtgctg gaagagtgcc tgcgggttgc cggctttcat
     4561 ttcttttcga tcggggcggc aagaagtttt tcgatgacgg ggatatcggc gcctgggacg
     4621 atatgatgac cattgatgat aaaatagggt gtgccttcga caccgagccg gcggccctgt
     4681 tcctggtgcg tcttcatcaa ttcctgtacc gccggctttt cgcagagggc cagcgttttt
     4741 ttatcaagga gaccccgata gctttcctta taagcctcct ggggatccgg agcgcagagg
     4801 atatgacgga ccttatccgc cgaggatttg gagaggggat aaaaaaagat gtagcgtgtt
     4861 aaatcgtctt tcccggacag tgcctgggcc gctttccggc agtagggaca gtccgggtcc
     4921 gtaatctcta ttatggtatg gcggccggag ccgaccttga gggccttgtc cagaggcagg
     4981 ttcttcgccc gcgccagaat cttctcgttg tggcgctgcc gggtaatgtt tctcccttcc
     5041 atggaaaaga tgtttccgat gatcaggcac gcggcatccg gggcgtaata gacgatgccg
     5101 cgggctgtct ccacttcata caatcccttg atgttcgaag gttgtaaaga ctcgtaatgc
     5161 aattggggga agtttttctt aagcgatgcg tccaaagatt cttccgtgcc ggccaccgag
     5221 gccgtgccac agcaaaccaa cccgatcagg aagacgataa ctcttttccc catgagacaa
     5281 atcctcttac ggtatagatt tttgatggtc atagtttctt catccaccgg agagttcacg
     5341 ttgaggtggc atcttgacga acagcgaaag gatgttccgg tcatgatccc gccgatattc
     5401 tacccggtcc atgagctgcc ggacaaaaaa tattcccagt cccccaatct ggcgctcgtc
     5461 gatgcccgca tttatatcgg gatcattgac ggagaggatg ttgaaaggta tccccgtgtc
     5521 ctggatccgg atctccagag tgtcctccgg accgggctgg aaaaccaaag ccacatctcc
     5581 ggttgtggcg gggtaggcgt aattgaaaat attcaccagc acttcttcca cgcaaagttc
     5641 gatttcatgg attcttttga gatcgaaccc tgcctttttt gtgaacgagg tgacaaaatc
     5701 gagcattttt tgcagataga caagctgggc gggaacaacg atgccggtcg aggtatccat
     5761 aaataccaaa gaggcttaaa gctgcttcaa agcctctgcc tcggtatcga agatagtgaa
     5821 gatcgatccg aatccggata ttttgaacac atctttgacc gggccctgaa gaccggcaaa
     5881 gaccagcttc ccttccttgg tcttcaaaac cttggcaata gcgagaatac tgcgcaggcc
     5941 ggcactgctg atatattcca aatgatccat cgccagtaga aaggtttttt ccccggcgcc
     6001 gatggattcc atgagattct tttcgaaatc aggcgccgta acggcatcga ttctgccgct
     6061 gacggaaacg atcaaaacat ccttttcctt ctttttattg atttccattg gccctcctcg
     6121 ctatcttttt gactgctttg cacgggtaag acaagaccgg ctccagaaca tcccggtgtt
     6181 tgcccgtatc tttgtacaaa atgataattg ccggaaattc ctgtcacaaa tttccgccca
     6241 tgtcaaaggt aatttcatgt cgctaattgc tcttgacgag acccttggtt tgtcatacat
     6301 tttgccaata aagaaacgaa cagggaggct tggataaggt ggacaagaaa aggtatgaat
     6361 tgaacgtcca gttggcgcag atgctcaagg gtggcgtcat catggacgtc accaacgtcg
     6421 aacaggcaaa gatcgccgaa gatgcaggcg ccgttgccgt tatggccctg gagcgggtgc
     6481 cggcggacat tcgggcacag gggggagtgg cgcgcatgtc cgatcccgcc atgattgtgg
     6541 aaatcaaaaa ggccgtctcc attcccgtca tggccaaggc caggatagga catttcgtgg
     6601 aggcgcagat cctggaagcc ctgaaaatcg actacatcga cgagagcgag gtcctgaccc
     6661 ccgccgatga agaatgccac atcgacaaga ccgcttttcc cattccgttt gtttgcggcg
     6721 cccgcaatct gggtgaggcc ttgcgccgca ttgccgaagg ggcggccatg atccgcacga
     6781 aaggggaagc gggtacgggc aatgtcgtgg aagcggtgcg ccatatgcgg accatgaatc
     6841 gggagatccg ccagttggcg cgcctgcccc gggaagaggt gacgggcttt gcgaaggcga
     6901 atgggattcc ttacgaactg gccctgcagg tacgggaact gggccggttg ccggtagtaa
     6961 actttgccgc cggcggcatt gccacgcccg ccgacgccgc cctgatgatg cagcttgggt
     7021 gtgacggcat ttttgtcggc tccggaattt tcaagtccgc ggatcccgcc cggagggccc
     7081 gggccattgt caaggcgacg gcctattttc aggacccgca gaagatcctg gaggcgtcca
     7141 tggatctcgg cgaggccatg ccgggattgg aaatcggctc cattccggaa aatcaactcc
     7201 tggccggaag aggctggtaa aaggcattgc cggtgatgat cggggttctc gacttgcagg
     7261 gagacgtggt ggaacatctc gatcacctgg accggctggg ggtgtccgcc cggcgggtta
     7321 aagatcgatc cgatcttgcc aatctccagg ggctgatcat tcccggcggc gaaagcacct
     7381 gtctggcaag acttctggta attttcggcc tcgatgcggc catcaggtcc gcttaccggc
     7441 gggggatgaa gatctggggc acctgtgcgg gcgccattct gcttgcgtca cgggtcatgg
     7501 ggggaaaggc gcatctgggt ctcatcgaca tggaaatcga gcggaacgga ttcggaagcc
     7561 agttggacag cttcaccggc agagctgtca tcccgaaggt ctccaaggta cccgttcccc
     7621 tgatcttcat cagggcgccg aagataagaa gcgtcggtcc cggggtggaa atcctttggc
     7681 aggcggagga ttatatcgcg gcggtggaga ctcgggaagg gctggccacg gtattccatc
     7741 cggaattgac caacaacctt gcctttcaca gctattttgc ccgtaagtgc ggcctcgata
     7801 tcgatacccg gcccgcatcg ccttcggaag aagattcctc ttggacaatc accagttgga
     7861 cccgccacat gcgcattgca ccgggatgac aaggtcgccg ttaccgcccg tccaccaaag
     7921 cactctcatg cccatagcgg gcacaacgaa taattaaaaa accggctact tggggacatt
     7981 gccgtgtaag ctaattcccg ttaccacctc atcccttgaa gggcggggat ccaaacatga
     8041 acagatcgac atgggcatgg tgaggaacca ctttcgtgga catcatgctt tgcattccct
     8101 tccaggaggt cgttttgggc ggcggcataa ggtgaaggca gcagcttgct gtcggtccgg
     8161 tcaaaatttc cctttagaaa cgataaaaaa gctgcgtttt cccgatccgg atgcacggat
     8221 tgaatcttct ggaacttccc gtcgtcaaat gattccagca gagcggtctg gaccgcatag
     8281 cggttgagat aggctctgcc cagcaacacc aggtccccgc ttttcttgat ttcctccacc
     8341 gcgcgctgaa aatgaagttc tgcgatggtt gtccttccct ccagagccgc ctgcttgaag
     8401 tcctccagca tgttgaatgc ggcataggtc caatcgggga cgtttccgga accggcacaa
     8461 cccgtgatca gggccgtgaa gataaagcat aaggcgatct tcttaatatt attataattc
     8521 atagggcaac tcccaaataa gcattcgatc tgaaatcttg acctgttaag cagtttttcc
     8581 ttgccaagaa actggtcaag gccttcattt atccctgcat acacttcacc ccgcttaaca
     8641 acggccgtct tttcccggag ccggaaacct gcttgtttcc tcagggcagt tttatcttcg
     8701 gttccgattt gaacggcatt ttccggttca gttcatcgag gagacggttc agggaattta
     8761 ccgtggcatc gatatcgcta cgcaacagct tgagatcctt ggtggagccc gcggcatcgg
     8821 aggttatttt ggtgatgttg tccagggtgg caccgatctt ggcgagattg ttcgccagtt
     8881 ctctcagggc cttggtgacc agaggcagga tgccgtcctt tccatatatg tcctcatcgg
     8941 tcttcttcag gagaatattt acctgatcgg ttatttgccg ggtgttccgc agggaggcgt
     9001 gaatagcctg aacgctttta cgatccccca gggccggcat catttcaaaa ggccgatcca
     9061 gaaccaggat ggaatcctcc agcatcaccg cccgcaacac catcacacaa aaacgctgtt
     9121 cctcccgtaa cgttggattt agcctgccca ccagatcttc caattgcggt ctccgtaaat
     9181 attcccgggc catctcctcg gcccggcggc gggataggcg actgtgatat tgcctgataa
     9241 gggcgatatt ctccaggacg ctgagattgg agatcaaggg catctcccgg gacaccaaac
     9301 ctaccggatg acggtatgcc ataaaggcgt gcagttcctc cttcagccgc ttttcgctgt
     9361 cgaagaacct cagattcaga tcaattttgt gatcatgggc gtgatgacta atgatagcac
     9421 ctcgatgata acgataacga tggcgataaa aacatttacc atcccgatca agaccgcggc
     9481 ggagatgctg gttaattcgt ttgtagcccg gattccatga cggatgggga tcatgacgat
     9541 gaagaagccg aagacggcac ttttgacgac catggcgatt aaatcgaaaa aattggccga
     9601 ttccaataat gcggcgatat aatcgcgggc ggacatattg aaaaaaagcc ggagtaaatc
     9661 aagccacagg taaggacaat gagggcaaac gggcaggtaa gcaggacaac ggaagcgatg
     9721 ccgctgatta cccgtggcgg gaagagataa ttgatgatgt cgattcggaa aaccgccagg
     9781 gtattgagtt cgttattgac cttcataacg gcgatttcgg cgttgatggc tgaactggaa
     9841 cgaagggcga tgaggagcac cgttacgaga ggggcaatct ccgcgaccat gaagcccatg
     9901 agcaagcgac caaaaaagga aaacaacccg atgcgcagca gatattctcc aatgatgccg
     9961 acaaaggcga tactcaggca gaccgccgca gtaaaaaaag cggcaggatc tacaccgcct
    10021 tgaagtaaat ctgattgacc agtaccatgg atacggcgct gttgtatgcc cggcgatcga
    10081 aaaggcgggc aaagatcagg taggaaaagg acatggcgtc ccgtagggaa cgggcggctt
    10141 gaagaccttt ttcgccgata ctatccaaga cgttaagcat gcccgaatat gtcattcttt
    10201 ccggctgggc ctcaaggcgg attatttggt cagcacgatg aggaaaaacg gaccgggaac
    10261 ctcctggagg gatacggcgg aaaaacccgc ccggcaggca aaattcgtcg cctctccagc
    10321 cgtgtagctg ttgccgaaga cgacgttcct aaggcaccag tgcattacag gcggccagac
    10381 gtaccaggcg cagtatttat ccgcaaactc cctcagcccc gcgggggagg tgttgcggtc
    10441 cacttcggcg ataaaggccc gggatcgagg ccgcaggacg cgcctgattt ccgtgagccc
    10501 tcgcacgggg tcgggccagt gtttgatgga gttgacactg atgaccaggt cgaaactctc
    10561 ggcggcaaag gggatatcca tggcattgcc gagctgaaag cggcaattgt caagcccagc
    10621 cttttttttc atctcttggg cggcccggac ctgtccggga gagtaatcga tgccggtaat
    10681 cgccggcgac gggagggttc gggccaacag caaactcatg gttcccgcgc ccgcgcctac
    10741 atcgaggatc tgtatggggg tcgcgcccgt attaacttct ctttccaacc gaccggcaag
    10801 gttagagaaa tagtccgctg ccgccgggcc gatcaggcga ttgtaaatcg gaccctggaa
    10861 gaggtcccag gcccaggcgg aatcgtggat gcgcttgaga agaccctttt ctccggcgtc
    10921 gatacgtttt cctcctcttt atattttcta cttttacttc gatatgacca ataaaaaaag
    10981 gtcgtcattg ctatttcccg ctctttcagg gcgaatgcta tggatcttaa ctactttttt
    11041 gattttgatc tgtcaagaaa cgattcgaca gggaaatccg gccggagatc ttgacggccg
    11101 gggaaaatgc ccctatactt cccggtaggg gagtcctcgc tccgggcttg gggtttcgga
    11161 gcccttgacg acaacatgaa aaaaggagag cagaaagatg aaaatttacc actgtcttgc
    11221 cgatgacgga cggcgctatt atggacagct ccataaagag gatcaagggt tgctcgaggt
    11281 gtgggaagga aacctttttg ccccctggac gcccacggag accgtggtga aaatcaacaa
    11341 actgttgccg cctctgctgc tgccgcccaa catcttcgcc ctcggcttga gctaccgaag
    11401 gcacgccgat gaaacgggaa tcgaggttat tcccgaaccg ctcgtattta tgaaggcctc
    11461 caccagcgtt atcggacctg aggatacgat cctccttccg gacgccggac cggcgaaggt
    11521 ggactacgag ggcgaacttg cggtggtaat cggcaggcgg ggcaagaata tcccgccgcc
    11581 ggaggctatg gattatgtcc tgggctacac ctgcgccaat gatgtaagcg ccagggattg
    11641 gcagttcgaa aggcagcagg ggcaatgggt ccgcggcaag agtttcgaca gcttctgtcc
    11701 cttgggaccc tgtctggtga cccgggatga acttcccgat cccaacaagc ttgccgtccg
    11761 taccgtcctt aacggccaga tcgttcagga cgcctccacc aacggcatga tctacgatat
    11821 tgccaccata gtcagcaacc ttagtaaatc cctaacctta ctgccgggaa cggttattct
    11881 cacggggacg ccggaaggag tgggattcac cagggtgccc cccctgtttc taagggatgg
    11941 agaccgcatt tccgtaacaa tcgaaggcat tggcaccctt gccaaccccg tgcgggccga
    12001 acgtatcgat gcttaggaat tcccgttgcc accatgcctt tcgccctccc gtgggcagcg
    12061 gttccgtcaa cccttgcgga ggggaaaatt tgccggccgg atcccgatgt gaatagagat
    12121 aatcacattg aattttatgg aaagttatgg tagggcgcga caaaatcaaa ttcattgacc
    12181 gggtcctgag ggaggaataa ccttttttat ggaaagcaag aatcgcaaga cttttttcat
    12241 gtttttactg gcctgcctta tcggcttctt tctcgtgtcc gtggtcgagg tgctgcgttc
    12301 ttccttcctc gtgcccacgg ggacggatgt aatgccggct tccgccataa caccggggga
    12361 tgggagtcgg gcgcccggct cttttgccga tcttgcggaa aaagtaaaac cggccgtggt
    12421 caatatcagt acgaccaagg tcatgaaagg cggcgggtat cgttccccat ttggcggcac
    12481 gcctttcgac cgctacttcg gggacgattt cttcgagcgt ttctttgggg attcgccacg
    12541 ccgggaattc aagcagcaga gtcttggttc aggttttatc atcagttccg acggatacat
    12601 ctttaccaac aaccatgtag tcgaacaggc ggataaaatc ctggtcaagc tgtccgatgg
    12661 tcgtgaatat gaggccaagg tggtgggtaa ggatgccaag accgatatcg ccctcattaa
    12721 aatcaaagct ggcgacagtc tgccggttgc ggaaatcggc gattccgaaa agctgcgggt
    12781 cggcgactgg gtcattgcca tcggtaatcc cttcggtttg gaacagaccg ttaccgccgg
    12841 catcgtcagc gccaaagggc gggttatcgg ggccggacct tacgacaatt tcatccagac
    12901 ggacgcctcc attaatcccg gcaacagcgg cggccccctc ttcaatatgg agggtaaggt
    12961 gatcggcatc aataccgcta tcgtggccca gggccaggga ataggctttg ccattcccat
    13021 cgccatggcg aataatgtcc ttcccgacct caaggccaag ggtaaggtaa cccgcggatg
    13081 gatgggagtt acggtccagg atattaccga ggacatggcc cgctccctcc agcttaaaga
    13141 ccgtcggggc gccatcatta ccgaggtctt caagggcgat ccggcggatc tggcgggatt
    13201 gcggccgggg gacatcgtta cggccatcaa cggcaagaag atcaaagaca cgcacgaact
    13261 gcttttgctg atctcctcct tccacgtggg cgacaaggtc agcatttcgg tcctccggga
    13321 cgggcgggcg atgaattttc aggttaccgt ggcggaacgt cgggatcggc cggagactgc
    13381 ccgatccagt cggggcggat acgagcatta tggactggcc gttggtgaca tcaccccgga
    13441 aatcgcccgt tatctgggaa tcccgaaaaa gacgggcgtg atcgtcatgc gcgtccagcc
    13501 gggcagtcca gccgatgaag tgggtattca accccaggat gttattctgc aggtgaacaa
    13561 ggtcaagatc tcctcggtaa aggattatct acgggaggtg aacaagaagg cggccagtgg
    13621 aagcgtgatg ctcctcatca agaggggaca ggccaccttc ttcgtcgcgc tgcgcaaata
    13681 aaggggaggc ttacaagcct ttgtcgaagc gagttttgtc tgttctccca tagttgtgtg
    13741 atagcgggtc ccggtctgct gaagatcgct ttatgccggc acggcggtag ggtcgggccg
    13801 gggtggaaac cgtattctgc cgccgccatg ggtggacgga taaacacaag caccgggaag
    13861 gtatcctgtc gtggataagg cgcgatggct tgacgacact tatcgaatcc tggacgatca
    13921 cttcggaaac cttcactggt ggcccggcga ttctcctctg gaagtcattg tcggggcaat
    13981 cctgacccag aatacggcct ggaaaaatgt cgagaaggcc attgccgccc tcaggcggca
    14041 tgcccttctt tcggtggcgg cgttggtgga aattccggag tgtgagcttg ccgggttgat
    14101 ccgtccgtcc gggtattaca atgtcaaagc ccggcgcctc aaggcctttt ttatctttct
    14161 tcgcgatgag ttccgtgggt ccttggaggt catgttttcc gaggatgtcc cgatgctgcg
    14221 gcaaaaattg ctggggatca aaggcatcgg cgccgaaacg gcggacagca tcctcctgta
    14281 tgccggtggt aaaccggtct ttgtcgtgga cgcgtacacc cgtcgtatcc tgagccgtca
    14341 tggcattgtc gccacccggt cttcctacgg ggaaatccag cgcctcttta tggatcgcct
    14401 tcccgcggat gccgctttgt tcaatcaata ccatgccctg atcgtttata ccggtaaaaa
    14461 ctattgtcga aagcagcccc ggtgccagga atgtccgctt tatcccttct ccgtaccgca
    14521 acatattgac atggacatga acggcaggca caagaaaccc cggaccgcaa ctgctctagg
    14581 ggaagttgtt tagcgggtga aaacaacctt gggggtgatg gagatgctga agaagatcga
    14641 tgattgccgt tgggaggtgc cccagtccgg ggggatgcgg gtccctggga tcatttacag
    14701 cagcgagcgc ctcctcaggg acatggggag cgacgaaagt caaaaacagg tggccaacgt
    14761 ggcccatctg ccgggaatcg tcaattattc cctggccatg ccggacgtcc actggggata
    14821 tggcttcccc attggcgggg tggcggcctt cgatgtggag aacgggataa tttcccccgg
    14881 tggggtgggt tacgacatca actgcggctg ccgcctcatg acgacccgcc ttgcctatga
    14941 agaaatacga ggaagcttgc gggaactcgt gacagccctc tttcaaaata tcccttccgg
    15001 cgtcggttcg aagggtgatt tgaaactgtc cgttaaagaa gagaaaaagg tcctcgaaga
    15061 aggggcggcc tgggcggtgc gccagggata cgggaccccc gcggacctgg aaaccactga
    15121 ggacggcggc cgcatggccg gggccgaccc ggaacaggta agcgaccggg ccctggaacg
    15181 gggccgggag cagttgggca ccctggggtc ggggaatcat tttctggaga tcgaggtggt
    15241 ggaggagatc ttcgatccgc aagcggccgc ggccttcggc ctggaactcc atcaggtcgt
    15301 ggtgatgatc cacagtggtt cccggggcct gggataccag gtctgcgacg actacctgtc
    15361 caggatggtc aagcatgtga atgagttagg cttttcactt ccggatcgac agttggcctg
    15421 ctccttcctg cgggatcgcc gcggccaaga ttactacgcc gccatggcgg cggcggccaa
    15481 ctatgcctgg gccaaccgtc aactcctcat gcactggacc agggaaacct ttgaaaagac
    15541 attgaaaaag agtccccggg aggtggggat gcgactcctg tatgatgtct gccacaatat
    15601 cgccaaactc gaaaccttct ccataggcgg ccgggaaagg caactctgcg tccaccgcaa
    15661 aggggctacc cgtgccctgc cgcccggcca tccggcgctt cccccggcat atagcggggt
    15721 tggccaaccc gtcctcatcc cgggtaacat gggcgccggt tcctatgtcc tggcggggat
    15781 gcagcgggcc ttcgaggaga cctttgccag cgcctgccat ggcgccggtc gggtcatgag
    15841 ccggagccag gccataaagg ccagcaaggg acgggcgata gtccgggaaa tggcggaact
    15901 gggggtaacg gtcatggcca gcggtcgtgg caccctgagt gaggagatcc cgggggccta
    15961 caagaacctg gaggaggtgg tcgcagtggt ccaccgggcg ggactcgccc ggaatgtggc
    16021 ccgccttcgg gccgtgggct gcatcaaggg ctgatggcgt cgagacgacg ccggaggaaa
    16081 aatcatgaac ttcggaacag cgtcatggaa agccgaccaa tgcgatattc atatcccttc
    16141 ccaagccctg gagatcggtc ttatcaatcg ggtcgtcgct gatgatgatt accaggaggg
    16201 actccgggct tttttcgaca agagaggcga agttcatagg gagatgatgt aggggactcc
    16261 gaaagcaaaa accccctgaa gaccggttca tcagggggtt ttcttatggg gacacagatc
    16321 tttaaaagac gtgtctttta tgaaacaggt cggtttgttt tcacacctct ttccgtttca
    16381 gccaggcctc ctggtagagc cggccgatgg agaggaagag ggccaggaag aacatcccga
    16441 acatgaagtt gggggccgtg ccgaagtact ggtcgatctt gaagcccagg tagaggaaca
    16501 ggaaggaggc gatggcgatg acgaagccgt aggcgctcac gatcatcacg gggttgtact
    16561 tgtggtaaag agcctttctg gtttcttgcc gcgctgccat ttctccttcc tcctttcaaa
    16621 gattgctgaa gatgtttgtt agccgaccag gtttttgatg ccttcgatca ccggattggt
    16681 gaagagggcg atgaggaccg tgtagagggc gaagaccccg gccacttcgc cggtgtacat
    16741 cttctcgtac ttgtgcttca ggctgatgat caccatgccg cccacgatca ggcagccaag
    16801 ctggaagtag gggaagttgg ccacgcccgc cgccgcatac tcggccaggg cgaaagtccc
    16861 ggtgcaaaga accaccacta ttgccgctac caaagttcct aatgtctttt tcatcttctg
    16921 tgtcctccgt ttattttaat tttttttgtt gtcttccgtt tgactttagg taatgcgagg
    16981 agcatgccaa aatcgacaga caacctttac acaaaggata tgtgtatgta tttgctcata
    17041 tattgtctgc catagccggg agaaaaaacc aaatttccgt cggattaata ggaccatttt
    17101 taaaaaatct ttcaacccgt ggaacgaaac gattgaaaaa gacgtgatta cgactttttt
    17161 ctcatggaaa aggaaagcag gacggcgtga agcggcgcca cccatcaaaa cgagaagcag
    17221 acggacaagc ctccgtagag ataggagctt tcccggtcca ctgccgatgc tgttcctttc
    17281 aggcccgttc ctttcatctc gtacctcgca tcgtcggaaa ggggaaacac ataagagatc
    17341 gtcggggtga ccgttatctg tccggtagcc ttgacgggga ggctcatggt caccgtgccg
    17401 tcatgaaaat tgttgaactt gtcgtccatc gccgccgcgt cgctattgaa tctgggatag
    17461 ccgccgtagc cctccccggc attgaaaagg gcggcatcgg cgtaggtgct caggagatag
    17521 cttgccgtcg ccgcaatctt cagggagacc ctcttggtaa agtcatgggt gtgggaaatc
    17581 cccaaaagaa aataccaatt acggtagtgg tccatttcct tgtaggccgt catggtcggt
    17641 gcaagaaggg tattgagact taccgaggcg aatatctcct gggcatcggc acgatccggg
    17701 gcatccttgt tgagggcgcc cagactgtaa tagatgtagc cggccccgag gttgaatatc
    17761 cccagcttct tggagtagga tatcgtaaaa tccgtttcgt tccaagaact tgcatagttg
    17821 gtatcggtgg cgcccgcaaa ataggggcgc atatccaggt tgccccagag accggcggag
    17881 aagcctttgt aaccgatcgt cgtcgccggc tggacgacga tactgtgccg gctcatttca
    17941 taaccccgcc agatgtattg gcttatggcc gccacggtga aatcgccggt gggtcgttct
    18001 tcctcgtcgg ccgcggcgga ggttgccacg ggcggtgttg ccgccgacaa tcgtgacacc
    18061 ggcgccccgg ttgtttccaa tgacgtggac atgtattttc ccgagcgtga gatttcttcc
    18121 gcccggacgg taaaactaaa actgaaaaga atcagggcgg acagcaggat agccgccgcc
    18181 gtctgtattt tagttgacat ggatgcttcc tccttcacca agtagtaaat aaaatttgaa
    18241 tacttcctat tcagcctgtt ttttagctta ttatggtttc atcgtatctg atgaagaaaa
    18301 ataatgcttt tcttcatctt cccgcaggga tgtgatcaaa tgggtgatgc attaacggtt
    18361 ctttccggga acaaagggtc cgggatgatc cttcagccga tggccacccc gccccgctct
    18421 ccggttctga tccgcagcac ctcgtacaga tccaggatga agatcttgcc gtcgcctacc
    18481 tcgccggtgt gggcgctgtc ggtaatggcc gccagggccg gggccacata atcctcgttg
    18541 acggcgatct ccagtttaac ttttttcagg aggctgcccg cttccttggc gccccggtaa
    18601 acctccgtga cgcctttctg ccgaccccgg cccaggacgt tggacaccgt caccaggggt
    18661 atttccacct tttctagggc ggccataact tcgtctaact tatggggctg aataatggcg
    18721 atgatgtatt tcatggcact gatccttttt actcaataat atcgtacatg ttgcctgtaa
    18781 tggattcgcg gtggcgggca tcggatttca ttcgatgacc gtgtaggcgg cttcgctctg
    18841 ctgggtcagg tcgaggccga tgttctcttc cctttctttg acccggacac acatcagggc
    18901 gtccacgatt ttgtagatga taatggtcat tatcacggcg tagaggggcg ccgcgaccgc
    18961 catgaccacc tggggccaga gttgcgcccc gccgaaaaag agcccgtccg ctccagaggc
    19021 gttgaccgcc ttttccgcga agaggcccac cgcgatggta ccccagatgc cgccgacccc
    19081 gtggactccg aaggcgtcca gggcatcgtc gtagccgagg cgggtcttga tcacggtaac
    19141 ggcgatatag cagacgaaag caacgacaat gccgatgaaa atggcattca tggggttcac
    19201 gaacccgcag gccggcgtga tcgccaccag acccgccacc gcccccgtga cggttcccag
    19261 gatcgtcggt ttgccatgcc gctgccattc gataagtgcc caggtcagtc ccgccgccgc
    19321 cgtgccgata tgggtgacca cgaaggcatt ggccgccagt tcattggccc ccagggcgga
    19381 gccggcattg aaaccgaacc agccgaacca gagcaacgcc ccgcccagga ccgtaaaggg
    19441 gagattgtgc ggtcggaaag gcgtcttttg gtagcccagc cgtttcccca agaggataca
    19501 aaggaccagg gccgacaccc cagaactggc gtggacgacg atgccgccgg caaaatctag
    19561 gccgccgagg ttcctcatcc agcccccggt cccccagacc cagtgggcca ggaggtcata
    19621 gacgagcgtc gtccagagga tcgtaaagac caggaaggcg gaaaacttca tccgttccgc
    19681 aaaggcgccg atcatcaggg ccggagtgat cacggcgaac atacattgaa agatcatgaa
    19741 gacgccgtga gggatcgtgg cggcgtaatc gccattgggc tgccccccca catgattgag
    19801 acccgcccac tccagaccgc cgataatccc ctgccccgta tcggcaccga aggacaggga
    19861 ataaccgaac aggacccatt ggagggagat tacgcacatg atgatgaagc attgcatgag
    19921 gacggaaagg acatttttcc ggcggaccag ccctccgtaa aagaaggcca gtcccgggat
    19981 ggtcatggca aagacgagga tcgtggataa cattacccac gccgtatcgc cggtgttcat
    20041 aaggcacctc ctgacaagaa atttccggtt agggataatc cgggaacatc aaggagtatg
    20101 ccaggggaat ttattggtgg ttaaggcgat gattttattt gtataatttc caagatagcc
    20161 tgaaaggtgc ggcgccggat ctggaacatg attgtcttat tggtgaagat acgaacaaaa
    20221 ttgtatcgag aattaaaaga taaaccaccc cttcgggtta gacgcccggc cgtgattttt
    20281 tctggccggc ttgcttacgg ccaccattgg tttcccaatg ctccgtatgt cggtgatccg
    20341 gtggaaatta ccggcaaaag acgattcaat ccaatttgtt attgttgcgc tgtctctcct
    20401 ggagcaggag aaaagtcgcg gagacccgac atacattccg gcctctgtcg tccgtgagga
    20461 ggcagtcgcc gatgatgagg ctttgaccct tcttgaacac cgtggccgta gcggtgaccg
    20521 cgccggaact taccgccgcc aggaagttgc ttttcaactc aatggtggtc aaaccttccc
    20581 ggtcgtccag ggtggtgacc atggcatggg cgatagcttc gtccgccagg gctaccatca
    20641 gaccgccctg cagcctgcct gcgccttgca ggtattccgg gcggaccggc agacgaaatc
    20701 tggcaaaacc ttctttgatt tccaagggtt cgatgccgat aaaatccata aagggattca
    20761 atccctttat acccgcccgt atattatcca gatatttctt gaagtcggga atggcaatcc
    20821 tccttatcgt cccaccgccg gcaccgatgc cgcggccccg ccgcaggtgg cgccggtcca
    20881 aggcggtagg gagttagcgg gcggcaggcg gaaaaagatc cgcccgccgt gctgcgattg
    20941 cctccaaagg tccgcctcag gcctccggac gtttctacag atcatagttg cccgccgcct
    21001 cgggctggta caggatgctg atgattttgt atgtccgctt cttccccgag ggaaggggcc
    21061 attgaatttc gtcgccggcg ctcagaccca aaagggcggc acccaccggc gccaggatgg
    21121 agatccgcct ctggttcacg tcggcctggg agggatatac caaggtggcc tcagtttctt
    21181 ctcctgtttc caactcccgg aagacgaccc gggaattcat ggtcaccaca tcggcaggaa
    21241 tctccgcgga tgggactatc acggcgcgtt ccagttcctc ccgtaatctt ctgatgttgg
    21301 gatggttccc tgctcccgcc accattggtt ccagacgctc catgtctttt tccgtgatga
    21361 atatattcct cttctcagtg gtcatggcga ttatcctcct ctggtcggag tgaaaggatc
    21421 attctttcct ccgggcgttt tgtgccggtg catgtcgatc gggagggaac ttcacctcat
    21481 gacccgattg gtatagtgca acatgtatct atccggcacg gcgcggatat tggcattaaa
    21541 aaacagcgtc tgataaggct attatgaaaa attgcttctg tcaacgcgaa aaggagttga
    21601 ttgcagggaa ggcaataaat ctggtaaaca gcccggacat gccgccggtg aattaccacc
    21661 ctaccggagg tgcgggaata tgtgtggggg agagaggttg tttggcgatg gccgaagggg
    21721 agatgaaata cggcgccctg gtccggacag ggatgctcat ggggatgcgt aagggatgga
    21781 gcgggttcgt ctggatgatg aagatcgtcc tgcccatttc tttgctcacg gcgctcctgg
    21841 aatggagcgg ctggctccgg cagctcgact tcctcctcac gccggcaatg agcctcctcc
    21901 atcttcccgc cgccgccgcc ctccccctga tcatcgggat gctggccaac atctacggcg
    21961 ggatcgccgc catggtcctt ttaccactaa cggaagggca gatgaccctc atcggcgtct
    22021 tcctccttat ggcccacaac ctcattcagg agggggtcat ccagggccag tcgggcatcc
    22081 atcccgtgaa ggccacgctg ttccgcattg taacggccgc ccttaccgtc atgatcatcg
    22141 gtccctgggt ggccggcgtt cccgccgtca ccccgccgcc gaccctgcgg gcagccaata
    22201 cagtcgccgc tccggcgggg gatcaagggg cgcggtgtga gcccatggtc gttccgactt
    22261 cgccgccgcc ggccgccagt gtgcctgccg gcgccacgaa cacgaaaata ccggcaaccg
    22321 tgcctcccgt ccccgtaagc aacgctcccg gctcaccggc cacaccatcg acgggaattg
    22381 ccgccgcgga tacttccccc gccctccccc ttacggacat gctccgccag tggttctgga
    22441 ccacggcggt cctctgcggc aagatcttcc tcatcatcat ggggatcatg accctgctgg
    22501 agacgatgaa gtttcttgac tggatcaagt acatcgtccg gctgttttcc ccgctcctcc
    22561 gggtgatggg cctggatgcc cagatgggtc tcctctggat gacggccatc gtcttcgggc
    22621 tggcctacgg cggtgcggta attgtcgagg aggcgaaggc ggagcacctc tccaaggacg
    22681 aactggagat cctccacctg tccatcggca tcaaccattc catggtggaa gacccgcccc
    22741 tgttcatctc cctgggtctt agccccttct ggctctacat ccctcgcctg atcgtcgccg
    22801 tggttacggt gcacctgatc cgtctgtggc gccgggttcg ccacccccag gcccggtgat
    22861 gataccggct tctggccgcc acctctctcg aagcccagca acgccgccgc cccggattca
    22921 aggccgcatg gtcctatggc aaaccaaaaa agccggaatt gggagccaca gcgaatctct
    22981 tggggaggaa acacgaaaat attccttgct atttccggga taggcctgta aacggtatcc
    23041 cgtgatcgcg atagttattt accttccctc ttttcttttt ccagaaactt cggcaggaaa
    23101 tccggctcat cgtaaaagaa tccatccata gacgcaggtt ttatcccgat ttttcggctt
    23161 cctatgagcc ctaacacggg atcgggagta cggccgtgcc gttcaactat ggattcatgc
    23221 gtcactaata cttgaaaaca gaaggaaaaa gaaaaaatga gatttgaaga gtttgcttta
    23281 aacgcgaaat tgctggccga agtgcagtct atgggctaca cgatgccgac gcctatacag
    23341 gcaaaatcca tcccgccgat tatggccggc cgtgatgtca tgggtcttgc ccagaccggt
    23401 acggggaaaa cggccgcctt tgtcctgccc attttacagc gactgatgtc gggacctcac
    23461 ggtccggtcc gcgccctgat catcgccccg acccgggagc tcagtgagca gacccataag
    23521 gtcattggtt tactgggccg gaagacccgt ctcaagagcg cctccattta cggcggcgtc
    23581 aatatcaatg gacagataaa gctcctccgt gatcggatcg acatcatctc cggatgcccg
    23641 gggcggctct tggatcacat tgaccgcggt accttggatc tgtccaagat tgaggtcctg
    23701 gtccttgacg aagcggatca catgttcgac atgggcttcc tgccggatat ccgccggatc
    23761 gtaaagcgcc ttccgggccg gcgtcagacg ctgcttttct ccgccaccat gcccgcggac
    23821 atccaaaaac tggccgatga tctcctgcaa aacccgctgc acataaaaat cgggcacaac
    23881 gcgcccgtag agagcgtgtc acaccacttc tatcccgtac ccatggacgt gaaaacggcg
    23941 ctgctcagag aaatactgga tcgtacgcac agggactccg ttctggtctt cacgcgcacg
    24001 aaatcgcggg caaagagtgt ggcgggccag cttggcaaat cgggatacaa ggccatttct
    24061 ctccacggca acctgaccca gcagaaacgg caacaggcca tcgacggctt ccgcgccggc
    24121 cgttacgaca tcatggtggc caccgatatc gccgcccggg gcatcgatgt ggccggcatc
    24181 tcgcatgtta tcaattatga tatgccggcg acgctggatg cctataccca ccgcatcggc
    24241 cggacgggcc gcgcctctgg aaccggcgaa gcctttacgt ttgtcaccgg tcaggacatg
    24301 gaactggcgc aggcgctaaa aagatccctg ggagacaaat tgacctatca ccgcgtcgac
    24361 ggccttacca ccatggtcat gccggataag cgggatgccg cggacatccg cccagaccgg
    24421 accggggccg gaaatcctcc cgacaaccgc aaggggggaa gatcattctc tccccggggc
    24481 caccggtccc ggtgcggaaa atcacagccg cggacactgc cgcccgcggg acaacgctcc
    24541 tggggtcatt gaggtccgtt acgaacggtg acgaccaaag cagagagggg attgcgccca
    24601 tgccttcaaa acttaggata ctatttagaa ccagatggtt tcattctttt ctctaccatt
    24661 ttattcggct ttacagcttg acgctcagga tacgggtcga gaatgaaaaa ggatggatgg
    24721 accatctccg gagcggcggc cgggtcgtgt tgtgcacctg gcaccagcaa ttctttgccg
    24781 ccatcggaca tttccagagc taccgcgact tcaagccggc actcatgatc agtaagagtt
    24841 ctgatggcga gatcatcgcg gatgtcgccg aaagaagcgg ttggcatgcg gtgcgggggt
    24901 cttcttcacg gggagggaag gaagccttga gaaaaatgat cgaccatttg agcctcaccg
    24961 ggctggccgg ccatattctg gatgggcccc gggggccggc ggggattgtg aaggcaggtg
    25021 tgatcgagct tgcccatgcc gccgatgcgg tcatcacccc tttttacacc tctgccgacc
    25081 gggcctggta cttcaacagt tgggataatt ttattttacc caaaccattt gccaaggtat
    25141 taatatcctt tggaaatatg atcaagctgt tgcccgtgga ggatgcgatc gcctttgagt
    25201 gtcagcggct tgaactggaa aaaaccatga ttcctgagtt gagggtaaaa cctgccggga
    25261 aatctgccca tatgtgatca tgccgccact agcttgatca agctttcaac gagacatttc
    25321 cattatccga tctggacgca aatttgattc cgattttttc gtgatcatcc tggggccgaa
    25381 atgattcccc cttgcccggg ggggcgtctt ttgctatata atcataaaca atgaaacagg
    25441 agaggatagt cccatgatct ataatgagga atttgaaacc ctgcccaggg aagccctgga
    25501 agccctgcaa ctcaagcggc ttcagcaagt ggtgcagcgg gtgtatcaca ccgtgggctt
    25561 ctacaagcgt actttcgatg ccgccggggc gacgccggac gacattcgga cgatcagtga
    25621 tctgcaacgc cttcccttca ccaccaaaca ggatcttcgg gacaattacc ccttcggtct
    25681 tttcgccgtt cccatgagca gcgtcgtgcg gctccacgcc tcttcgggaa ccacgggacg
    25741 gtccacagtg gtgggctata cccaccggga catcgaaacg tggtcggagc tcgtagcgcg
    25801 gtgctttgtg gcggcggggc tgaccaaaaa cgacatcatc cacaatgctt atggctacgg
    25861 cctcttcacc ggcggtctgg gcgcccatta cggtgcggag aaactggggg ccagcgtcat
    25921 tcccatgtcc gggggcaata caaagcggca gctcatgatc ctccaggatt tcgggcccac
    25981 ggccatctgc tgcaccccct cctatgccct gaatctggcc gagcagggag gggccatggg
    26041 ggtggatatg cgggccctga aactccgggt cgggatcttt ggcgccgagc cctggagtga
    26101 caagatgcgt gacgagatag aaaacgttat ggatatcacg gccctgaaca tctacggact
    26161 ttcggagatc atgggccccg gggtggccat ggagtgccag gaaggccggg agggaatgca
    26221 tgtcttcgaa gaccactttc tggtggaaac cattgaccct gcgacgggca aagtcctgcc
    26281 gccgggagaa acgggcgaac tcgtttttac caccctgacc aaggaggcct ttcccctgat
    26341 ccgttaccgg acccgggaca tctcccggct gatcaccgcc ccatgccggt gcgggcggac
    26401 ccatttccgt atggaccggg tcgagggaag aagcgacgac atgctgattg tcaggggggt
    26461 gaacgtgttc ccctcccaga tcgaggcggt tctcgttggc atccagggcg tcgagcccca
    26521 ttaccagctt atcgttgatc gcgagggcac cctggatacc ctggaggtgc aggtggaggt
    26581 gagcgacgcc ctcttttcca atgccgacga agtccgggtc ctccagaaga tcgaaagacg
    26641 catcagtaag gatttgaaag actatctcgg catcacggcg aagatcaagc tggtggagcc
    26701 caagtctatc cagcgttccg agggcaaggc cagccgggtg atcgataaac gacagatttg
    26761 acggggaggt aaaacccatg aaagtcgaac aggtatcagt gtttttggaa aacaagccgg
    26821 gctctctgga agaggtgacg aggattctca aagaggcgaa catcaacatt cgtaccctgt
    26881 ccctggcgga taccacggat tttggcatcc tccgcctcat tgtcaacaat gttgatacca
    26941 ccagccgggt cctccgggac aatggattcc gggtcagccg gaccaccgtc gtcgccgtcg
    27001 aggtgccgga tcgtcccggc ggtctccaca gcatcctggc ggtgcttaaa aacgacaata
    27061 tcttggtcga gtatctctac gccttcgtgg agaaaagcgg ggaaaacgcc gtgatcatct
    27121 tccgtttcga tgattccgac gccgccatcg aagtcctccg gaagaataat ttcaatgtga
    27181 tttcggggga gaaactgtac agcttctgaa gggacaaaag ataagcaatc aaccaaagga
    27241 gggtcgtgcg atgagaggaa aatcgatgcg ttcggcagaa tcgctggtgg aggaacaact
    27301 gggaaagtgg cgcctggccg ggaccgagag aaaaaaggaa aaggccaagg tgggtccggt
    27361 gataaccgtt tcccgggaat ccggctgcgg aggcaccaag atcgtccaga aactggccaa
    27421 agagctgggc ttcgatctca tgggcggcaa gatcattcag atggtggcgg aaagcgtcga
    27481 aatgagcgag aaggtcatct gctccctcga tgaaaaggag gttaccaaac gggacgactg
    27541 gctgaactcc ctttttgaaa cccgacacct ctggcccgac cagtacctac accacctaac
    27601 caaggttatt ggtaccatcg gccgtcatgg gaatgccgtg atcctgggcc ggggggccaa
    27661 ctatatcctg ccccaggaaa gcaccttccg ggtgcggatc atcggcccct tcgacctgcg
    27721 gatcaaaaac gcgacccgta ctacggaaga tgaggcccga cgctatctca tcaaagttga
    27781 tgcagaccgg gaggccttca tacgaaaata cttcaatgcc gacgtcaagg atcccgccca
    27841 ctatgacctg gtcatcaata tgcgggtcgt ttccatcgac ggagcagtga aagccgtcat
    27901 cgccgccttc aattcctgga aacgctgagt gtgcaggcaa tgatgcttga gacctaaatg
    27961 attctcaata tccgccgcaa acttttcctc ggttatctcg cgatggctct tctcaccatt
    28021 gtggcgagta catatgccgt gggacatctg cgctccctca acaacctggc cgaaaagatg
    28081 atccatcagg atgcccgcct cctcgatctg gaaaagaaga tgatggacgc cctgttagcc
    28141 tgggaagggg tggaaaaaag atatctgatc ctcaaagatc cctctctggc ggacatcttt
    28201 tggacccgga gcgaggattt tcaatcccag atagaaaaac tcaaagcaga ggccctgccg
    28261 gagcaggttc agaccatcac cgccctggcc acgctggcca agcaatacgc ggagatcttc
    28321 gtgcgtgaga ccgtcctggt ggatgaggat cgcctcgatg aagcggcggc cctgtcgtct
    28381 cacgaggggc gccgggtaat ggaaaagatg gggctggcat tgagggccct ccaggtacag
    28441 acggagaaga acatcgacag ccggatgaag gccataaaca tcaaggctct ggaagcgagc
    28501 cacatgacca tcgccctggc ggtggctagc cttcttatcg gcatgatttt ggccgctgcc
    28561 attaccttta atatatccag gcggttacga aaactcgaag tggcgacggg agatgtcgcg
    28621 gaaggtaatt tcgaccggga gatcgatatc cgaggggaag acgaaatctg tagccttgcc
    28681 gcggcattca ataccatgac caaacgcctc aaggttctgg aggccatgaa catcgatgcc
    28741 agccccctta cgggattacc gggcaatctg gccattgagc aggaaatccg tcgccgcctg
    28801 gaattgaaga aaccgttttc tctatgtcat cttgatctgg acaattttaa gccctttgcc
    28861 gatcattatg gatacgcctg ggggagcgag gtcatcaagg aggtggcggt gattctcgac
    28921 gagggcaagc ggattcgggg cgaggccggg gatttcatcg gtcatatcgg cggggatgac
    28981 ttcatcctta tcacggaacc ggcacgggcg gaagggctct gcggctttat tgtggccgag
    29041 tttgacgaac gagtccagag gttttacagc gaagaagacc gccggcgggg cttcatcctt
    29101 gccaaggatc gccaaggacg gcttcaggaa ttccccctga tcaccattac gatctccatc
    29161 gtcagcgatg acggttcgca gtatcataac cccctggaga tggccaagat ggcggcggag
    29221 gtgaaaaatt acggcaaatc cctggccgga agcaaatatg tccatagaga agacatgccg
    29281 ggctgaccgg gaaaagaaaa caacggcgaa agccatgaaa ataattgata ttacagagca
    29341 atctctttat cgccgactga tgcctctgct gctggccgcg gtcgtgaccg tcctggcggc
    29401 ctgtgcggcg aaaggtccgc cgcctccccc gggaagcctg gcggcccgat tccagtccga
    29461 taccgccctg ttccaggagg gatatgaacg actccgggga gtggatagac aaaaggatga
    29521 agcgatagcc cggcaggcat tcgtcctact aatggaacaa tatcccaaaa gcaaatggcg
    29581 tggttacgcc gagctgtatc tccgcctcct gaacgaagag caacggctcc aaaaagaatt
    29641 gcaggacctc cataaccggg tacaggcgga ccggcagggc gcggccgtcc gggaagcggg
    29701 gttggaaaag gagcggcagg aactgcgatc ccttctcgag caggagcgtg cagacgcggc
    29761 ccggctgcaa cgggagaacc agtccctgcg tcatgagaac gaccggcttc agaacaatat
    29821 cgactccctg aaacgactgg agatcgagtt ggatcggcgg gacaagctct accgctgaag
    29881 gaccagggag aggcccaggg ccgttttcac atcccgaata gaaccgccga ccagtaactc
    29941 gcgggcctcc gccgacgata ccctgacgat ggcgatgtct tcgtcggcgt cgggaggaag
    30001 gggggccgcc tccaggtcct cggccagata gaaatgcata tactcgttgc cgtagccggg
    30061 gaggagataa ccttcgaaaa gtttggtgat ccggctggcg cgataacccg tttcctcccg
    30121 taattccctt tggacgcaaa gctccggcgt ctcccctcgg tcgatgtttc ccgcgggtat
    30181 ttccagaaga taaccgcccg tggcatggcg gtactggcgg atcaaaagaa agttgccggt
    30241 ttcgtcccgg ggcaccacgg cgatgcaggg gttatggtcg atccaactca ggcgggagcg
    30301 gtggccgttg ggcagggtga cttcttcttc cagaaccttg aagatgcggc aggtatagac
    30361 aaccggcatg agcctttcct ttcgggtgat cagttcggtt ttatgaccgc caggaggcgg
    30421 cgttcgatct cttccttggg aagggcgccg acggcgcggc tgaggagctt gccatcccgg
    30481 aagaaaagca gggtggggat gttgcggatg tcgaactggg cggcggtgac gggattttca
    30541 tccacattta gtttggcgat ccggactcct ccggcgtatt tcgccgccag ttcctcgagg
    30601 atgggcgcca ctgtccgaca gggggcgcac cagggggccc aacaatcaac gatcacggtt
    30661 ccgggcgccg tcaatacctc gcgggtgaag gcggcgtcgg taatgtcgaa aggttggccg
    30721 gtgtcaccac cgacgaccag cgggacgccg caccggccgc agagcggtcg ttcattgagt
    30781 cttgtctccg gcatgcggtt tttcgtcccg cagctcagac agcggatgat catgtcatcc
    30841 aggggcgccc gacatttgcc gcaggtcggg cggtcctgca tccgttccct ggggatgcgg
    30901 tttttcgtcc cgcaattcaa acagcggacg atgatctgtt catctgatcc gggcatcgac
    30961 ggtctcacct ttctatcgcc gcctgcaccg ggatagggtg caggatccgg ttgagttccg
    31021 cagggttcat ttccgccaga attccccggt ggccggcatt gatgaggatg aggggcaaag
    31081 agagaatcgt ctcctcgata tagatgggca atcgctttct ggttccgaag ggagaggtgc
    31141 ccccgacctt gtagccggta tgacgctccg caactttggg atcgcaaggg ctgaccgcct
    31201 tgacaccgag aatccgcgcc agggatttcg tcgaaacgtc ccgatcgccg tgcatgagga
    31261 cgatcagggg gcggccgttt tcatcttcca tgaccagagt cttgatgacc ttgtgctcgt
    31321 ccacgcccag ttcccgggcg gagatccggg ttccgccccg gtccacgtat ttataagcca
    31381 agagccggaa aaccgcctca tgacttttca attcccgcat ggccggcgtc atggggaatt
    31441 tctctttgga catgagaacc tctgctgcgc tgtaaaatat tcattccgta cgggagagtc
    31501 aatattacgc tgactcacca tacccgaaaa tgcccgttat gtaaagatca ggactttttc
    31561 gctgttttgt tgtatatacg tcggcattta agggattgca ggagggcggc atgcgcgtca
    31621 tcatcatcgg cgccggcgag gtcggctatc acattgcaaa aaaactgtcc gaggagaacc
    31681 aggaagtggt cctcatcgac caggatcccg ccaagatccg gaggatcaac gagaatctcg
    31741 acgtgcaatc ctttctaggg tccggaacga gtccccaggc cctccgtgcc gtgggcatcg
    31801 aggaggcgga attgctggtg gccgccacgg acagcgacga ggtgaacctg attgcctgcc
    31861 tgctggcaag gaatctcaac cgtcacatgg tcaaggtggc ccgggtacgt aaccgggagt
    31921 atttgcagga gcagtcgctg ttcagcaagg acctcctcgg ggtggatctc ctcatcaaca
    31981 cccgggacat gatggtggag acgatcctgg gactcatgga agtccccggg gcgtcagagg
    32041 tcatcgattt tgtgggcggc aaggtgaaga tggtgagctt cctcatctcc ggcgactcgc
    32101 ccctggccgg caaaaagctc agtgatttga aggcctatga gggtaatctc ctggtcgggg
    32161 ccatcgtccg gggccacaag atcgtcatcc ccagcggccg ggataccatc cagccccacg
    32221 atctggtcta tttcgtcatg aagaagaccg aactggaagg ctccctggcc cagttcgaca
    32281 tggcgaaccg gcccatgaaa aacatcatca tcgtcggggc gggggagacg ggaatggcca
    32341 tcgccaaagc tctggacgag cggcgcctga acgtcaagat catcgatcag gacggcgaaa
    32401 gatgtaaaaa gctggccggc atcctgaagc gggtgatcgt gatccggggt gacggagcag
    32461 accgggacct cctccgggag gaaaatgccg gagagatgga cctgatcatc gccgtgacgg
    32521 gggacgagga gagcaatgtc ctgatctccc tgctggccaa ggaactgggc ggccggcgca
    32581 ctatcacccg ggtgagcaag ctgagttata tgtccatcgt ctccgccgtg gggctggata
    32641 cggtcatcaa cccccgcatg gcggcggtgc gggcgatcct ccagtacatc cgtcggggcc
    32701 ggatcctgtc cgtggcgccc ctgcgggagg aacaggccga ggccatcgag gcggaggcca
    32761 tggaaacctc ggatatcgtc aacacccccc tgtgccgggt caacttccct cccggcgcca
    32821 tcctgggcgc catcgtccgg ggcgaggaga tcattattcc ccagggtgac accatcgtcc
    32881 ttccccatga ccggatcatc atcttcgccc tccaacaggt ccttcccaaa cttgaagaac
    32941 tgctgactgt aaagctggag tatttttgat gaattttcgc ctcatcgcct atctggtggc
    33001 cgttctgttg ttctgcatcg gactgtccat gacggcgccc ctgggtgttg ccctctatta
    33061 cggcgacggc agcgccccgg ccctgctggg ttctctttta ctgacctgcg gcgccggggg
    33121 cggggtattt ttccgaaccc ggggacagga ggacttctat ctgagccatc gggacggggt
    33181 ggccatcgtc accctgggct ggctggccgc gggcctggcg gcgacgctgc cctatctgct
    33241 ttccggggcg attcccgact ttacgaatgc ttacttcgag agcatctccg gattgacgac
    33301 cacgggatcg agcatcttta aggacgtcga agtgctgccc cagggcatcc tcttctggcg
    33361 cgcccagacc cagtggctgg gggggatggg gatcatcgtc ctttccattg ccatcctccc
    33421 ctttctcggc atcgggggca tgcagctcta caaggccgaa acccccagtc ccgtggtgga
    33481 caaactgact ccccggatct ccgataccgc tcaggccctc tggaagatct acattgtcct
    33541 cactataatt cagatcttcc tcctcatgtc cggggggatg gactggttcg attccatctg
    33601 ccataccttc gccaccatgc ccaccggcgg cttttctcca aaaaacgcca gcgtcggcca
    33661 ctaccaaagc gtctatatcg atttcgtcat cgtcctgttc atgttcgtgg cggggatgaa
    33721 tttctccctg cattacaagc tggtgaaagg gaatgtccgg cagttcttcc gggatccgga
    33781 atttcgctcc tatctgttca tcaccgcggc gatcatcctg gtgatcacca tcgatcttta
    33841 cggagccgcg atctacaagg ggatcttcga tgcctgccgc tatgccgcct tccaagtggt
    33901 gtccatcatg accaccaccg gtttcgtcac cgcggatttc gagcagtggc cggtattgtc
    33961 ccagcaactc ctgctgctct gcatgtttct ggggagcatg gccggatcaa cggggggcgg
    34021 catcaagatc atgcgcatcg tcctgctgct caaacactgc tatctggaga tcttccgcat
    34081 catccatccc catgccacca ccgtggtgaa gctgggggag atacccgttc cccagaccat
    34141 catgcggagc atctggggtt tttttctcct ctacgtgggg atttacatcg tcgccacggt
    34201 ggcgatggca gctttggggc tggacatgat ctcctcgatc tccgctgtgg ccacctgcct
    34261 ggggaacgtg ggtcccggtc tgggcagcgt gggccccatg gacaacttct ccggggtgcc
    34321 cgtcctgggc aagtggctcc tcatcttctg catgctcctg gggcgcctgg agatctacac
    34381 gatcatcgtc ctgctcatgc cgggcttctg gaggaagtaa gactgaatcc ggcggttcgc
    34441 gatcaacaga tcttggaaga acggcatcac aaggaaaaac tacttttccc tcgtcggcta
    34501 ttacggcttt cacggtgaag agatcctgac ggaaaacgat ccgccgggga cggatttcct
    34561 ggcgtccctg gccgccg
//
LOCUS       DAOSMF010000013        33434 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_1727_length_33434_cov_21.266904, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000013 DAOSMF010000000
VERSION     DAOSMF010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 33434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33434
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1727_length_33434_cov_21.266904"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(183..1481)
                     /locus_tag="PLT63_03055"
     CDS             complement(183..1481)
                     /locus_tag="PLT63_03055"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOD97731.1"
                     /translation="MDLGPVSATILGLLIVFMGGSIWIGISLFIVGIGGFFFFSDVSF
                     GSILSNIAWNNTSSSTMMALPFFIWMGEILFRSKISENLFKGLSPWMDSIPGRLIHVN
                     ILACTFFAAVSGSSAATTATVGKITVPELKQRGYDYALSIGSLAGAGTLGFMIPPSMV
                     MLVYGIIGDVSIGKLFIAGFIPGFMIAGLFSGYIFTRCLINPALAPAGSEHHTWRDRR
                     EAIPAIAPVIILIFAVLGSIYLGWATPTEAGAIGVVGAFFFAALTRSLTWSVFKESLI
                     NSVKTSCMIMLIVMGAAYLSNVFGFLGITRALTTFVTEMGLSPYILIVILTVLYLILG
                     CLIDGFSMIVMTAPLVLPLIEAAQFDTVWFGIYLVVMIELAQITPPVGFNLFVISGLN
                     NDSLFRIAKAALPFFWLMLLATVLLTVFPQIALFLPGMMK"
     gene            complement(1561..2097)
                     /locus_tag="PLT63_03060"
     CDS             complement(1561..2097)
                     /locus_tag="PLT63_03060"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOD97732.1"
                     /translation="MKRLIKIIDALSGANGWLAGIMTVLALILVITEIVWRTGFSKTI
                     YISDEYAGYLMTMLTFCGLAYTLRERGHIRMMMLPHFIKGRARIIFDMVCFVVGFIFC
                     VAFTWFTFVFFWDSYVNGSRSMHVSETYLAVPQFFMPLGAALMTLQFFAEFLKAIARL
                     RNDTEGLRILEESDELGR"
     gene            complement(2227..3219)
                     /locus_tag="PLT63_03065"
     CDS             complement(2227..3219)
                     /locus_tag="PLT63_03065"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HOD97733.1"
                     /translation="MKKLLSVFLVSVVFIFFIVAPAVAKTTWNANSVWPPKNNHSIGL
                     EAFAKKVKTATNGELELVVHSGGALGYKGPELLKVVRDGLVPVSDMLISGVAGDEKLL
                     QIVTLPFLVRNFDELHTLINIARPYFDKSAEKWGQKILYTAPWPGAGLWTKKEVKTLA
                     DLKGLKTRTYDKNGALVMEAVGATPLALPFSEVYTSLQTGVIDSVMTSSPTAVDAKFW
                     EVLKYFEPLNITIATDMVTVNKKAFDKLPKAQQDALVKAGQEMEKIMWAGVKKWDGSM
                     VGISKKNGIKVVAPSKKLVADLEKITENIRAEWFKTAPPEAQKIYAEFRKKVKR"
     gene            complement(3401..3676)
                     /locus_tag="PLT63_03070"
     CDS             complement(3401..3676)
                     /locus_tag="PLT63_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOD97734.1"
                     /translation="MRHVHVTAAGRVQGVAFRAWTRKTALSLQLTGWVRNLPNGSVEA
                     VFEGRDEQVELMVRRCAAGPPLADVTVITTREEAYRGEFTGFVIRYD"
     gene            complement(3673..4626)
                     /locus_tag="PLT63_03075"
     CDS             complement(3673..4626)
                     /locus_tag="PLT63_03075"
                     /inference="COORDINATES: protein motif:HMM:NF014663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HOD97735.1"
                     /translation="MDAAVTIRNPGLMTMIVDLGRNGYSDMGVPSSAALDGYACRALA
                     ALLDSPDTPVLEVTGPRFALRFDAEVTCAITGARVKAFLDDVPLLPWASFRAGRGSIL
                     RVREITEGFRYYIGFAGGLKIDRVMGSYTTNLECRFGGYEGRTLMGGDVLSLAAPGQP
                     DIRILPDHWIPPMASPHRLRVISGPESDYFTSDSLATFWDKDPQELFSTSRHLNRTGI
                     RMKGKPLLFRPEVPKSIISEGILPGTVQIPGDGLPIVMLYERTIGGYARVATVIRADR
                     DRLAHLRPGDMVYFERITIEEAEASWKEKKDRMEAFPLSGK"
     gene            complement(4630..5274)
                     /gene="pxpB"
                     /locus_tag="PLT63_03080"
     CDS             complement(4630..5274)
                     /gene="pxpB"
                     /locus_tag="PLT63_03080"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HOD97736.1"
                     /translation="MIVQRYTENALRIIFGSVIDLEIHEQVRRAFFICRSLDRREILE
                     IVPSFRACVIIYDEERISFPELAALLQERLADLSSVEMPPPRIHKIPVRYGGEYGPDL
                     SFVAGHCHLRPEEVIARHTGELYTVYTVGFTPGFPYLGTLDAAIAAPRLETPRTRIPA
                     GSVGIAQRQTGIYPFESPAGWQIIGRTDASLFDTKHPPYSLMQIGDKVRFYEVP"
     gene            complement(5271..6053)
                     /locus_tag="PLT63_03085"
     CDS             complement(5271..6053)
                     /locus_tag="PLT63_03085"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003814.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HOD97737.1"
                     /translation="MNTAIDLNCDMGESYGAYTIGGDAGVVAYITSANIACGFHASDP
                     MVMDRTVRLCRDNNVAPGAHPGFPDRVGFGRRFMDLTAEELINDVIYQVGALRGFLAR
                     YELPLQHVKLHGALYNYVATREELILRIAGALQKPFDNPVFLTLGTTRTTKLLEKCRR
                     EGIRLALEAFPDRRYTDAGELQSRREPGAVLKDPREIARRAVAMATRKGVESVSGRWL
                     EMDIHTLCLHGDNKESMEAAALIYRSLLDEQIQIHPLGVILP"
     gene            complement(6050..8092)
                     /gene="ligA"
                     /locus_tag="PLT63_03090"
     CDS             complement(6050..8092)
                     /gene="ligA"
                     /locus_tag="PLT63_03090"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOD97738.1"
                     /translation="MMSAGNDPDIAAAAARIEELRELIAYHNRRYYRLDDPEISDAAY
                     DRLLGELRDWEERYPELVTAASPTQRVGAAPLEKFTAFSHPSPMLSLANAITEADIRV
                     FHNRLLAGGNTISYVLEPKLDGVAVNLVYEGGILVAGATRGDGAVGEDVTHNIRTIAG
                     VPLTLADGATTEIPARLEVRGEVVIATAAFRRLNREREEAGEASFANPRNAAAGSLRQ
                     LDSRITARRPLDFFAYAVGIKEGGAELKTQWEVLAALKAWGFQVHPFARLAESIESCL
                     EGYRSLLAQREELNHEIDGMVLKVNELSLQAELGSIFRRPRWALACKFPARQETTVIE
                     RIDVQVGRTGALTPVAIMRPVRIGGVTVSRASLHNQDEIDRKDIRLGDTVVVQRAGDV
                     IPEVVKVVPGRRTGREIPFLLPASCPVCGTAVVRLPGEAAHRCINIACPAQVKERIVH
                     FAARGGMDIEGLGGKMVSRLVDAALIKDPADIYSLTAVKLLALERTGEKSVGNLLLAI
                     AASKNPPLEKLIFALGIRQVGESMAKTLARHYLGLKELADADADELTAIRDLGPEAAG
                     SITRFFREPANKRFLAKLEAAGIAPVNSLYSPSSPTVGSLAGKIFVITGTLTGMTREE
                     AKHRIESRGGKTTESVSKNTHYVVAGASPGSKLEKAQALGISILDEPTFFALLETP"
     gene            complement(8089..8385)
                     /locus_tag="PLT63_03095"
     CDS             complement(8089..8385)
                     /locus_tag="PLT63_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97739.1"
                     /translation="MLGKTLERIAEKILGLDEASLASLWKKYKERMEHFDASRDWERA
                     VIVFFIINAVRAKNQIFNEEVRKQQPAPTPLPPHKSGAVGRKKPALKLIKPDKT"
     gene            8508..8993
                     /locus_tag="PLT63_03100"
     CDS             8508..8993
                     /locus_tag="PLT63_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HOD97740.1"
                     /translation="MKAIGAPWRMTYIRGEKVKECVFCVDGATCPGGDLRLLEGKRAF
                     VMMNRYPYTSGHLMVIPRRHVSSLEELTAAERAEIFDLVALSVQALKESVRPDGFNIG
                     MNLGKAAGAGIDDHLHVHVVPRWNGDTNFVSVVGDVRVIPEDVAQTWEHLRPYFKKSA
                     R"
     gene            9032..9340
                     /locus_tag="PLT63_03105"
     CDS             9032..9340
                     /locus_tag="PLT63_03105"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HOD97741.1"
                     /translation="MKIVYTVIVVVLIMFIVTFSLANTFPVQLRYLDVINVTLPAYML
                     IFVCFLVGVVFTGFMGIVERFRLNRTISQLNKTIRELRREIRANEPPPIIEEQGPPAP
                     "
     gene            9403..12012
                     /locus_tag="PLT63_03110"
     CDS             9403..12012
                     /locus_tag="PLT63_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97742.1"
                     /translation="MKKKIIIIAIMLVVAGPIVLAAVLLTLMQSPRAVNALAAALEPI
                     TGIHLHVHDLAFDHHLRGHVQGLQIRAGHERGFAVSLARAEIDGNLDLGFKLRAERIV
                     LEGPKFTFHLKDEESESDPFAVLGKIPPVKQLVVKDGHLELKAPGAVYSIPGLEMTIN
                     DFDPTGHGKLSGRSSFHIQAGDMDTRGVGEAALEFHRFSPRPSAFGSLRFSLADGTYG
                     NMKLTGAVLASAIRLDDGLLTLNNVHAAVEELARREGPSPAIIRNLQADLNASYDQRT
                     SRFAITNGKVYGASLGALNGRISGTIKPLSWHASLQAPVLDLAGVFTLARPLLPAEYR
                     GWNCKGRGGLEVESRGKTQDGTTVWEADVAVDFREGGFASADGTKAGERISGRVELKL
                     EAPEKGRPGNFRFSMESGGGELLWASYYNDFRGEKVKIASRGDFAPNPFYLAADGTCD
                     IFQSGDYSFSARFSPEQNILSLRARGISLPRLFSTLARNFLQQNYPELQDLRVEGEAD
                     LELTVVAAPEQKTIAGEFDLHGGSLQSPFHRLVLAGIEISLPYDLALAGKAVTPPPAD
                     LRRGSMAFSGFEKGDIRIKGFATPVILSGNRFTLPEPVTVPIFGGVVRLAGLQAEDLL
                     RPEMRLKTFLEIHGIDLEELTGPALPIPLSGVINGGFSPLVFQKDLWTASGVIVVETF
                     GGRIAVDNLFGGRLLSKARFFGGDAGFESIDLEALTASIEIGRMTGLIRGGLNNFRME
                     YGQPSRFELVILSDESRNMPRRISVDAIENLSIISTGSGAVSSLMSSGLNMFFKDYPY
                     SRIGIRCALADDIFSLQGLIHEGGTEYLVRRAWLRGIDIINQNPDNSISFKDMAERVG
                     RIRQPRQEEKGVS"
     gene            12063..12635
                     /locus_tag="PLT63_03115"
     CDS             12063..12635
                     /locus_tag="PLT63_03115"
                     /inference="COORDINATES: protein motif:HMM:NF018701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HOD97743.1"
                     /translation="MRAICKVCGCLAILFVAACVTVNVYFPAAEVQKAADKIVDDVRK
                     KTGETPAAPSSGVEIWKRFGFGVQPAYAQMNIDISTPAIRGLKDAMKNRFPQLKPLYD
                     QGAVGENNNGLIELRDAAALSLAQRSQANRLIDQENSDRNALYGEIAQANKLGPESVA
                     QIRKIFANSWREKSLAGWWVQNDRGVWEKK"
     gene            complement(12814..13302)
                     /locus_tag="PLT63_03120"
     CDS             complement(12814..13302)
                     /locus_tag="PLT63_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HOD97744.1"
                     /translation="MIRAAIVTVSDKGSQGQREDLSGPEAARILATAEIEVLSIQIVP
                     DEVEEIKEILIELADRQEADIIVTTGGTGVSPRDVTPEATRAVLEREIPGMGEAMRRA
                     GLEVTPHAMLSRALAGIRGRTLIVNLPGSPRGVRENLAVILPALSHAVAKIQGSDADC
                     AI"
     gene            complement(13304..15391)
                     /gene="fusA"
                     /locus_tag="PLT63_03125"
     CDS             complement(13304..15391)
                     /gene="fusA"
                     /locus_tag="PLT63_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOD97745.1"
                     /translation="MAKYDSKSLRNIVIVGHGSTGKTSLGESCLYVSGKTDRLGRVDE
                     GTSSLDYEPEEQKKRISISAATTSVEWQKCKINIIDTPGDSNFSFDTQSCLRIGDAAI
                     VVIDAVGGVEFQTQKVWEYTDLYHLSRMVFINRMDRERADFQTAVASIKEKMDVKITP
                     ICLPIGAHDSFRGIVDLMTMQALIYDDLKGTFKTTDIPADMTEEAQKWRETMVEDIAE
                     SDEAMMDKYLDAGELTDEELRDGLKSGVHSGAIIPVACGSALKNIGTGLLMDMIVRYL
                     PSPVDRGPVKGKKPDGGEEETRLPEESAPFSALVFKTIADPYAGRLTLFRVYSGVLTP
                     EAGMYNASRKLSERFGNIFFLEGKTQKPTDMVIPGDIAAFAKLKETGTGDTICAEKNP
                     IIYEKVTPPPTIMSFAIEPKSRGDEDKIVSSINRLIEEDPTMHFHRDDQTKEMIISGM
                     GQIHIEVAVEKMRRKFGVEVNLKTPKVPYKETIKGKTNVQGKYKKQSGGRGQYGDCWL
                     DIEPLPRGTGFEFQDKIVGGVIPQQYRPAVEKGIVKAMEEGVVAGYPVVDFKVSLVDG
                     TYHTVDSSEMAFMIAGSMGFKKGVLACQPVLLEPIVNIFIEIPDEYMGDVIGDLNGRR
                     GRVQGMDTKGSIQIIKGQVPLAEILKYAPDLRSMTSGRGTFTYEHSHYEEVPAHIAEK
                     IIAESKKKEEAEK"
     gene            15575..17035
                     /locus_tag="PLT63_03130"
     CDS             15575..17035
                     /locus_tag="PLT63_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HOD97746.1"
                     /translation="MKKRNRKISKVLIANRGEIALRIMRTVQEMSLDVVVVYEHADAE
                     AYYVRQANEAVMIGEGPRKDYLDIDKIIWAARKTGADAIHPGYGFLAENPEFSAACEK
                     AGIIFIGPPPQVIRDLGNKVVARNIMERAGIPFIPGTADILKGGDGVNEAFSFARACG
                     YPVMLKASCGGGGRGIRKVKDEADLMVQLPLARSESLSAFNDDRIYMEKCIECPRHVE
                     IQILADKHGNIIHLGSRDCSIQRRHQKLLEIAPADLPEDVLEAMYECAVRAAREAGYI
                     NAGTVEFLVDARTNEFWFMEMNTRLQVEHTVTEELTGIDIVREQIRIAEGAVLNIPKE
                     RIHLHGKAVQVRINAEDPKNNFMPEGGKRVEVYQSPGGPGVRLDGAVYQGYRIPTDYD
                     SLLVKMTIRGYNWEQTLQRLKRSLRGFIIVGPKTTIPFYLAICDDKDFRAGKFDTGYI
                     DTHPEIFEYPEAEREIAKLGKLIAEIHARKINRHAY"
     gene            17061..19019
                     /locus_tag="PLT63_03135"
     CDS             17061..19019
                     /locus_tag="PLT63_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HOD97747.1"
                     /translation="MKKIRKKELYREGEPRITPREMRDKGVKYVLDKIKRQPGYYVTN
                     TERDLSQSDFKNRIMPHTQLLVAEERNQAGFFSLEITGGASVHVDMLRKQINPLEKLQ
                     VLGERMPDTMFQTLCRGINLFGYRPYPDNVIRMTVRTFARYVHVWRVFDFLNHIPNMV
                     PVFEEVKAAGCILEPSICFSTGPEHTDAYYVRKVGEILDVTGEDILLCIKNHGGLGTS
                     KRIGDLVRAIHDRYPDLVIHYHGHNTDGEDIGRIVAAVLNGAKIVDASDHAFTGFYGP
                     PPLLSVVDILADYGYQAAGLDRQAVIETSNKLRPEREYYRDFESQFLGFDPTVQIHKL
                     PGGATGSSFEQAVKGGFLQKMPEILQKELPRVQVELGNWWSVTPGSQILWTTAVNNVL
                     KGERYKDANDDLKNLMLGRYGDLPFYMPSDEVYRTVFGPDWKQIVECDTCYQKIEDVD
                     LEVERKVLQHRLGRTATDDELTLYLQHPNDAVDFFKFEATYGKTWVLSPRIWFRKGGF
                     SLGEKFDIPDAEGKLHSVEIGPQRKTKSGDTATYLIIDHHPEPIVTEQEEEEGSKKQK
                     KTIMSPKEIDAAWKAGDIRSHLTGTVNDISVEQGEEVAQGQVLIVLEAMKMLNNITAE
                     ISGKVSEINVSPGDKVTIGDKLLFVAKD"
     gene            19145..21661
                     /gene="secA"
                     /locus_tag="PLT63_03140"
     CDS             19145..21661
                     /gene="secA"
                     /locus_tag="PLT63_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOD97748.1"
                     /translation="MIGALLKKIIGTKNDRIIKEISVVLDEINRLEPAMASRTDAELR
                     AKTPEFKERLLKGSSLDDLLPEAFAVVREAARRTVQMRPFDVQIIGGIVLHQGKIAEM
                     KTGEGKTLAATLPLYLNALTGKGCHLVTVNDYLARRDAGWMGPIYNFLGLSVGVIVHG
                     MEDEERRQAYAADITYGTNNEFGFDYLRDNMKFSLEDYVQRDFNYAIVDEVDSILIDE
                     ARTPLIISGPSEESTDKYYRINQVIPRLRKDTDYAIEEKTRTVVLTEEGIARVEKYLK
                     VQNLYEPKNIELLHHVNQALKAHTIFKRDVDYVVKDGQVIIVDEFTGRIMPGRRYSDG
                     LHQALEAKEHVKIERENQTLASVTFQNYFRMYDKLAGMTGTADTEAEEFKKIYKLDVV
                     VIPTNMPMIRTDHNDVIYKTEKEKFHAVIEEIKEMHRQGRPVLVGTISIVKSELLSAM
                     LTRTGIKHHVLNAKHHEREAEIVAQAGQRSAVTISTNMAGRGTDIKLGEGVADLGGLH
                     ILGTERHESRRIDNQLRGRSGRQGDMGSSRFYLSLEDDLLRIFGAEKISAIMDRIGIE
                     ENEPIENRLVSKAIENAQKRVEGQNFDIRKHLLEFDDVMNKQRQVIYEQRRKVLSGQD
                     LWTDIEGMVEETVADIVPDYVDEKGHPEDWDLMSLDDRIYQRFSLRLNLHEKKGEMSA
                     PEIEGAITAAVNSHLRRKEENFGVPLMQYIMTMIMLQSIDAQWKDHLLGMDHLKEGIG
                     LRGYGQKDPVREYQKEGYDMFMAMIQRIKEDTLEKLCLVQIRREEDIEEIQRQAEDDY
                     ILSRGEDVSATPVKREKEKVGRNDPCPCGSGKKYKKCCGR"
     gene            21681..22874
                     /gene="argJ"
                     /locus_tag="PLT63_03145"
     CDS             21681..22874
                     /gene="argJ"
                     /locus_tag="PLT63_03145"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF003802.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HOD97749.1"
                     /translation="MKEHAMAVPGFLASGVSAGIKETGAKDLSLIYSETPARAAGVFT
                     TNAFPAAPVVLSRQRIRCGYAQAILTNSGNANAATGEDGYRDALAMGGALARRLKIDK
                     DFVLVSSTGVIGHRLPLDKIRKGIPKLVAGLHPEGIKNAEEAIMTTDRFPKLSFRKRM
                     IGGREISVCGMAKGAGMIQPHMATMLAYVMTDADIDAKALDRAFRYGVERSFNAISVD
                     GCMSTNDTALVLANGVAGNRRIEERGRGFIAFRTMLAEVMTELARDMVRDGEGATKLL
                     EITVEGARTAGEARKMAYAIANSNLVKTAFYGGDPNWGRIIAAAGAMGVPLPLERVEL
                     LFDGVSLFSRGLKTAGNEELLKEVMSRAEIKVIFRAGMGNKSFRVFASDLGFEYIKIN
                     AHYHT"
     gene            23068..23889
                     /gene="rpsB"
                     /locus_tag="PLT63_03150"
     CDS             23068..23889
                     /gene="rpsB"
                     /locus_tag="PLT63_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOD97750.1"
                     /translation="MAHISMKLLLEAGVHFGHQTNKWNPKMKPYIFGARNGIYIIDLQ
                     QTVVLFHKAYEFVVDMVANGDELLFVGTKKQSQESIREEAGRCSMPYVNQRWLGGMLT
                     NFITIKKSIDRLNTLDKMIEDDSIRAFPKKEILSLQKEKAKLEKILGGIRKIKGRPGG
                     LFIVDPKRESITVKEARKLGIPIVAMVDTNCNPDDIDYIIPGNDDAIRAIKLFSAKIA
                     DAVLEGKKKFEERIQAESDKEGPVREVAIDNEEENDVPESIETKDSVSVAAHAEE"
     gene            23935..24540
                     /gene="tsf"
                     /locus_tag="PLT63_03155"
     CDS             23935..24540
                     /gene="tsf"
                     /locus_tag="PLT63_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOD97751.1"
                     /translation="MNITVEMVKTLRNKTNAGMMECKEALKAADGDFEKAVDYLRKKG
                     MSAATKRSSKAAKDGTIAHYIHMGGKIGVLVELNCETDFVAKTEAFQTMARDLAMHVA
                     ASNPLYVRPQEISADALEREKEIYKSQLLTEKKPEKIWDKIIEGKLRKYYEEVCLLQQ
                     KFIKNTDITVETLVNEMIAKTGENIIVRRFARFQLGEEIKS"
     gene            24540..25259
                     /gene="pyrH"
                     /locus_tag="PLT63_03160"
     CDS             24540..25259
                     /gene="pyrH"
                     /locus_tag="PLT63_03160"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005328724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOD97752.1"
                     /translation="MVKKAVYRRVLLKLSGEALEGERSFGIDFDVVNEIAQEIRAVVE
                     LGVQLGVVIGGGNIWRGGEAAKRGMERSAADYTGMLATLINCLAMQHALEHLGVATRL
                     QSALEVREIAEPYIHRRAIRHLEKGRVVIFAGGTGNPFFTTDTAASLRAVEIRAEVIM
                     KATKVDGVYDRDPVGNPDAVMYKIITYNEVLAKNLKVMDATAISLCRENGLPIIVFNL
                     RKKGNIAAAICGGKLGTLVEG"
     gene            25264..25821
                     /gene="frr"
                     /locus_tag="PLT63_03165"
     CDS             25264..25821
                     /gene="frr"
                     /locus_tag="PLT63_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOD97753.1"
                     /translation="MKEVVYEELKDNMEKILGSLEKGFSKVRTGRASLALLDGIKVDY
                     YGVPTPLNQVASLSVPESRLIVISPWDTGVIGAIEKAIQKSELGLMPSNDGKIIRLPI
                     PPLTEERRKDLVKIVKKLAEEGKVKMRNARRDANEQFKSLKKENKISEDEMFTAQDEV
                     QKRMDKYIEKLEAVLAVKESEIMEI"
     gene            26067..26813
                     /locus_tag="PLT63_03170"
     CDS             26067..26813
                     /locus_tag="PLT63_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HOD97754.1"
                     /translation="MADIDLQKLPRHIAIIMDGNGRWAKQHALGRIMGHQKGVEAVRK
                     TVRLCREIGIQYLTLYAFSVENWLRPDQEVAALMQILRQYLRSELAEMMENDIRLHAI
                     GDIDGLQAPVRKLLAETMARTAGNRGMVLNLALSYGGQDEIIRAVRRIAEDVREGTLA
                     AEDITRESFAAYLYTAGIPDPDLLIRTSGEFRISNFLLWQSAYTEFYFTEVLWPDFDK
                     KDLFAAIADYQRRERRYGLTSEQLQKGASS"
     gene            26810..27616
                     /locus_tag="PLT63_03175"
     CDS             26810..27616
                     /locus_tag="PLT63_03175"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOD97755.1"
                     /translation="MNSHIKRWLTAIVVVPVLFAVIAWGGETGFFALIVAVILLGIHE
                     YSNMVLDASQVNEKIQALVISALIPVVFFLGSPEMLTGLLCAAMLTVFILYLARIKKE
                     NFDLTPVMKIIFGVFYVSFLLSHLIWLRRGESGVLWIFFVIVLAFSGDVAAYYVGRTW
                     GRRKLMPLVSAGKTVEGVLGLVAGSALGCLIFSWFFLPQLFPWHAIVLGVFASVLGQL
                     GDLCESVLKRAAGVKDSGSLLPGHGGILDRLDCLLFIVPCVHYYRYYFIS"
     gene            27613..28785
                     /locus_tag="PLT63_03180"
     CDS             27613..28785
                     /locus_tag="PLT63_03180"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOD97756.1"
                     /translation="MKNIAILGSTGSIGTQALAVIDCNRDRFRVTALAAGRNVVLLRE
                     QIAKFRPRIVAVFDEECARRLGSEPGLPRELKILSGPDGYNEIAALPENDLVLSAMVG
                     AAGLVPTVTAVEAGKDVALANKETLVMAGALVMEKAAAKGVHILPVDSEHSAIFQCLQ
                     GGRAGEVRRIILTASGGPFRTWIREELRRVRPEQALRHPNWTMGRKITIDSATMMNKG
                     LEIIEARWLFAVKVEQIDVVVHPQSVVHSLVEFCDGSVLAQLGQPDMRLPIAYAFSWP
                     ERLAGTGAFLDFRSIPALEFHPPDADRFPCLSLAGDALRIGGTMPAVLNGANEIAVQA
                     FLEERILFTDIPEVIAGVMKSHRVVFAPTLRDIMAADSEARKRAVKMIEEKKDWLA"
     gene            28773..29879
                     /gene="rseP"
                     /locus_tag="PLT63_03185"
     CDS             28773..29879
                     /gene="rseP"
                     /locus_tag="PLT63_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOD97757.1"
                     /translation="MVGISILSVIVLLGILIFVHELGHFLMAKYFDVGVQKFSLGFGR
                     KLIGKKIGETEYLLSLVPLGGYVKLLGEGEGEELSAEEERRSFSRQSVWKRITIVFAG
                     PLFNFMLAILIFTGVYMLGVPVLTTTVGGVQEGSAAYEAGMKPGDVVQAIDGDAVGRW
                     DRMAVKITESAGKEMRFTVLRQGVPLELQIVPREMKGKNIFGEETTSFKIGVSPASLT
                     VIERQNPALAMWTALKQTWFITKLTLISIVKILEGVISPRTLGGPILIAQMAGAQVKE
                     GLIPFVLFMALLSINLGVLNLLPIPVLDGGHLLFYFVEAVRGKPVGMKTRERAQQIGF
                     VLLVMLMIFVIMIDIDRLDIKIINDFTKIFTNEP"
     gene            29876..30541
                     /gene="tsaB"
                     /locus_tag="PLT63_03190"
     CDS             29876..30541
                     /gene="tsaB"
                     /locus_tag="PLT63_03190"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOD97758.1"
                     /translation="MKLLALDTSGKTVSVAFVSDDVTVGEIFFDVERHHSEILLPAID
                     HLLKGAGVRIGDLDLFACTVGPGSFTGVRIGVSTVKGLALAYDRPIAGVSTLEALAVN
                     AAGFPTLVCPLIDAKRGQVYAGFYRSDTAGSLTCLSPDCLLAAEEIPNRIEGDAVFVG
                     SGAGVYKELISARSEGRSLFAPLHFHRISAAAVGIVARRKAEAGDLHSAVQLAPVYLR
                     REM"
     gene            30753..31394
                     /locus_tag="PLT63_03195"
     CDS             30753..31394
                     /locus_tag="PLT63_03195"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HOD97759.1"
                     /translation="MTHDSPIAPEGVPFIISAVLITSVCLYFQWTWAAVVFGLTTLWV
                     VWFFRNPRRTPPADPRAIISPADGKVIRIETVDGHELLPGPLNKVSIFMNVFSVHVNR
                     IPFGGVIDEIRYYPGKFLTANLDKASEDNERNALRIDIGGGEKILVIQIAGLVARRIV
                     CWTAKGMKVNKGERFGLIRFGSRLEVFMPPRAQLRVQVGEKVRAGETPIGELP"
     gene            31391..32185
                     /gene="pssA"
                     /locus_tag="PLT63_03200"
     CDS             31391..32185
                     /gene="pssA"
                     /locus_tag="PLT63_03200"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HOD97760.1"
                     /translation="MKEHSRFQKEKMKKGIYVLPNLFTTASLFAGFYSMIASMKGLFE
                     VAAVAILVAAVLDALDGRIARMTNTTSKFGAEYDSLADLVSFGVAPALLAYTWSLQDF
                     GRWGWVVSFLFVTCGALRLARFNIQIGIIDSRVFNGLPIPGAAAVVATGVLIYYKLGG
                     VGHFHHPLVLISVFILALFMVSNIKYYSFKDLNFFAKKPFMSFVVIVCILVVVIAEPQ
                     IMIFTFVAGYSLSGPIWLIVKLYRKGTSAATEKKSAAVKTGPQSSH"
     gene            32211..33227
                     /locus_tag="PLT63_03205"
     CDS             32211..33227
                     /locus_tag="PLT63_03205"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOD97761.1"
                     /translation="MKAFNVAVVGATGAVGNEMVKILEERNFPVAKLLLLASERSLGK
                     SLEYKGQSLPVEVLKEDSFTDVRIGLFSAGGSVSEKYAPLAARAGCVVVDNTSAFRMA
                     PDIPLVVPEVNPEAIAQYKQRGIIANPNCSTVQMVVALKPIHDAVRIKRIVVSTYQAV
                     SGTGKKAIEELSNQTRALLGMREPAVKVYPHRIAFNCLPQIDVFLDNGYTKEEMKMVN
                     ETKKIMKDDSIAVTATTVRVPVFYAHSESINIETEKKITAAEVRELLEKAPGVKIVDD
                     PRNLVYPLAIDAVGRDETFVGRIREDESITNGINMWVVSDNLRKGAALNAVQIAEILV
                     RDYL"
BASE COUNT         7140 a         9261 c        10117 g         6916 t
ORIGIN      
        1 agatcgaggt gcgctgctgg gagagccttg acgcccggat cgccgcgcct acccagctcc
       61 cctgggaaac cctggaaaag atggccgggc agattataag caaacttcca gaagtggtca
      121 gcgtgaccta caacatagcg acgaaaccgc catccaccat cgaggcggtt tagtcgcgcg
      181 ctttatttca tcataccggg gaggaaaagg gcaatctggg ggaacacggt gaggagtacc
      241 gtggctaaga gcatgagcca gaagaaaggc aaggctgcct tggcgatccg gaagaggctg
      301 tcgttgttca ggccgctgat aacgaagagg ttgaaaccca ccgggggggt gatctgagcg
      361 agttcgatca tcacgaccag gtagatcccg aaccagacgg tgtcaaactg ggccgcctcg
      421 ataaggggca ggacaagggg tgccgtcatg acgatcatgg agaagccgtc gatgagacac
      481 ccgagaatca gatacagaac cgtcaggatg acgatgagta tgtagggtga aagtcccatc
      541 tccgtgacga aggtggtcag cgcccgggta atccccagaa agccgaagac gttggaaaga
      601 taggcggccc ccatgacgat gagcatgatc atgcagctcg tcttgacgga gttgatgagg
      661 ctctctttga agaccgacca ggtaaggctc cttgtcaggg cggcgaaaaa gaaggccccg
      721 acgacgccga tggcgccggc ctcggtgggt gtcgcccagc cgagatagat acttcccaaa
      781 accgcaaaga tgagaatgat caccggggcg atggccggga tcgcttcacg gcggtcccgc
      841 caggtatgat gctcgctccc cgccggggcg agggccggat ttatcagaca gcgggtgaag
      901 atgtagccgc tgaagaggcc ggcgatcatg aagccgggga tgaagccggc gatgaaaagt
      961 ttgccgatgc tcacgtcgcc gatgatcccg taaacgagca tgaccatgct gggggggatc
     1021 atgaagccca gggtcccggc gccggctagg gagccgatgg agagggcgta atcgtatccc
     1081 cgctgtttga gttccgggac ggtgattttt cccaccgtgg ccgtggtggc ggcggaagag
     1141 ccgctgacgg cggcgaagaa ggtgcaggcc aggatattga catggatgag tcgtccggga
     1201 atgctgtcca tccagggcga cagacccttg aagagatttt ccgagatctt gctccggaag
     1261 aggatctccc ccatccagat gaaaaagggc agggccatca tggtcgaact gctggtgttg
     1321 ttccaggcga tgttcgagag gatggagccg aaggagacgt cgctgaagaa gaaaaagccc
     1381 ccgatgccca cgatgaacag ggagatgccg atccagatcg atccccccat gaagactatg
     1441 aggagaccca ggatggtcgc ggataccggt cctagatcca tggcgttttt ttcctctccg
     1501 gtgtttccgg cgtggcgaat tccgacggat gccgtttatc ccctgagttt gccccttctt
     1561 tcatcttccc agctcgtcgc tttcttcgag gatcctcaac ccctccgtat cgtttctgag
     1621 ccgggcgatc gccttgagga actcggcgaa aaactggagg gtcatgaggg cggcgcccaa
     1681 gggcatgaaa aactggggaa cggccagata ggtctccgag acgtgcatgg aacgggaccc
     1741 gttgacgtag gaatcccaga aaaagacgaa ggtgaaccag gtgaaggcaa cgcagaaaat
     1801 gaagccgaca acgaagcaga ccatgtcaaa gattatgcgc gcccgtccct tgatgaaatg
     1861 ggggagcatc atcattcgga tatgaccccg ctcccggagg gtgtaggcga ggccgcagaa
     1921 ggtgagcatg gtcatcagat aaccggcata ctcatcgctg atatagatgg tcttcgaaaa
     1981 acccgtccgc cagacgatct cggtgatgac gaggattagg gccagcacgg tcatgatccc
     2041 ggccagccag ccgttggccc ccgagagggc gtcgattatc tttatcaatc ttttcatgat
     2101 catcaatgaa aagggacccc gaagacctca ctccggggtc cctgcgcatg atctaaaatc
     2161 accggtggtg attcatgctt gcaaaaaagc tggaatagac ttatccgctt tatccgcagt
     2221 tgcgtcttag cgtttgacct tctttctgaa ctccgcgtag atcttctggg cctcgggcgg
     2281 ggctgtcttg aaccattcgg cgcgaatatt ctccgtgatc ttttccaggt cggcgacaag
     2341 tttcttggaa ggggccacca ccttgatgcc gtttttcttg ctgatgccga ccatgctgcc
     2401 gtcccacttc ttcacgccgg cccacatgat cttctccatc tcctgcccgg ccttgaccag
     2461 ggcatcctgc tgggccttgg ggagcttgtc gaatgccttc ttgttcacgg tgaccatatc
     2521 cgtggcgatg gtgatattca gcggttcgaa atacttgagg acctcccaga acttggcatc
     2581 cacggccgtg ggggaggagg tcatgacgga gtcgatgacg ccggtctgca gggaggtgta
     2641 aacctcgctg aaaggcaggg ccaggggcgt ggcgccgaca gcttccatga ccagggcgcc
     2701 gtttttgtca taagtgcggg tcttgagccc cttcaggtcg gccagggtct tcacttcttt
     2761 cttggtccag aggccggcgc cgggccaagg ggcggtatag aggatctttt gaccccattt
     2821 ttcggctgat ttgtcgaaat agggacgggc aatgttgatc aaagtgtgga gttcgtcgaa
     2881 attacggacc aggaagggca gggtgacgat ctgaaggagt ttctcatccc cggccacccc
     2941 gctgatgagc atgtcggaaa cgggcaccag gccgtcccgg accaccttga ggagttcggg
     3001 tcccttgtag cccagggcgc cgccggaatg gaccaccagt tccaactcgc cgttggttgc
     3061 ggtcttcacc tttttggcga atgcttccag gccgatgctg tggttatttt tcgggggcca
     3121 gacggaattg gcgttccatg tcgtcttggc gacggcgggc gcgacgatga aaaagatgaa
     3181 aaccactgat accagaaaaa ctgacaataa tttcttcata gaactcctcc ttttcttcat
     3241 gtttgtgcgt ccttgacgcg gtttacagac taaaaaactt gcgaactata cgaggatgaa
     3301 aacggtaagt caaggtaaaa aagagccatg ccggaccccg aaatcacaac tcgcctaccg
     3361 ccatcaactc catccggcag cacggaggcg gcgaaccgcc tcagtcataa cggatgacga
     3421 accctgtgaa ctccccccga tacgcttcct cccgggtggt gatgaccgtt acatccgcca
     3481 aggggggacc ggcggcgcat cggcggacca tgagttccac ctgctcgtcc cgcccctcga
     3541 agaccgcctc cacgctgccg ttggggagat tgcgcaccca acccgtcagt tgcagcgaca
     3601 aggccgtctt tctcgtccaa gcgcggaagg cgaccccctg gacgcggccg gccgcggtaa
     3661 catgcacgtg cctcatttac ccgacagtgg gaaggcttcc atgcggtcct tcttttcctt
     3721 ccatgacgcc tccgcttcct cgatggttat ccgttcgaaa taaaccatgt ctccaggtcg
     3781 caggtgcgcg aggcggtccc gatcggcccg gatcactgtg gccactcggg catagccgcc
     3841 gatggtcctt tcgtacagca tgacgatggg caggccgtcg cccgggatct gcaccgtgcc
     3901 cgggagaatc ccttccgaga tgatgctttt cgggacctca ggccggaaca acaggggttt
     3961 gcctttcatg cggatgcccg tccggttcag atgtctggat gtcgaaaata gttcctgggg
     4021 gtccttgtcc caaaaagtag ccaaagaatc agaggtgaaa tagtcgctct ccggcccgga
     4081 gatgacccgg agccggtgag gagaggccat gggggggatc cagtggtcgg gtaagatccg
     4141 gatatccggt tgccccggcg ccgccagcga caggacgtcg ccgcccatca acgtccgtcc
     4201 ctcatagccg ccgaatcggc attccaaatt ggtcgtgtag ctgcccatga cccggtcgat
     4261 cttgaggcca ccggcaaagc cgatgtagta gcggaagcct tccgtgatct cacggacgcg
     4321 gagaatgctt ccccgtccag cgcggaaaga agcccagggg agcaagggga cgtcgtccag
     4381 aaaggccttg acccgagcgc ctgtgatggc gcacgtcacc tcggcgtcga accgtagggc
     4441 aaaccgcggc ccagtgactt ccaacaccgg ggtgtccggg gagtccaaga gggcggcgag
     4501 ggcccggcag gcataaccgt ccaaggcggc cgaagaaggg acccccatat cgctgtaacc
     4561 gttgcgcccc agatcgacga tcatggtcat gagaccggga ttgcggatgg tgacagcggc
     4621 gtccatagtt caggggacct cgtaaaagcg caccttgtcg ccgatttgca tgaggctgta
     4681 tggcgggtgc ttggtatcga agagactggc atccgtgcgg ccgatgattt gccagcccgc
     4741 gggggattca aaagggtaga tccccgtctg gcgctgcgcg atgcccacgg agcccgcggg
     4801 gatccttgtc cggggggttt ccagacgtgg ggcggcgatg gccgcgtcga gggtgcccag
     4861 atatgggaag ccgggtgtga agccgacggt ataaacggta taaagttccc cggtatggcg
     4921 ggcgatgact tcttcggggc ggaggtggca atggccggcc acaaaggaga gatcaggtcc
     4981 gtactcgccg ccgtagcgga cgggtatttt gtgaatccgg ggcggtggca tttccacgct
     5041 ggaaagatcc gccagccgct cctggagcag ggcggcgagt tccgggaagg aaattcgttc
     5101 ctcgtcatag atgattacac aggcgcggaa agagggaacg atctccagga tttcccggcg
     5161 atccagggag cggcagatga aaaaggcccg ccggacctgt tcgtggatct ccagatcgat
     5221 aacggagccg aagatgatcc gcagagcgtt ttcggtgtag cgttggacga tcatggcagt
     5281 atcacgccca gggggtgtat ttggatctgt tcatcgagaa gggagcggta gatcagcgcc
     5341 gccgcctcca tgctctcctt gttgtccccg tggaggcata gggtatggat atccatttcc
     5401 agccaacggc cgctgacgct ttccacgccc ttccgggtcg ccatggccac cgcccgtcgg
     5461 gcaatctctc gggggtcctt gagaaccgcc ccgggttccc ggcgggattg cagctctccg
     5521 gcgtcggtgt agcgccggtc cgggaaggcc tccagggcca ggcggatgcc ttctcgacgg
     5581 catttttcca gcagtttcgt ggtcctggtt gtcccgaggg tcaaaaatac tggattgtcg
     5641 aagggttttt gtaacgcccc ggcgatcctc aagataagtt cctcccgagt cgccacatag
     5701 ttgtacaggg cgccgtggag ctttacatgc tggagcggca gttcgtagcg ggcgaggaaa
     5761 ccacggaggg cccccacctg atagattacg tcgttgatga gttcttcggc ggtcagatcc
     5821 atgaaccgcc gtccgaaacc tacccgatcg gggaagccgg gatgggcgcc cggggcgaca
     5881 ttgttatccc ggcagaggcg gacggtgcgg tccatcacca tggggtcgga ggcatgaaac
     5941 ccgcaggcga tgttagcaga ggttatgtag gctaccaccc cggcatcacc gccgatggtg
     6001 taggccccgt aggattcccc catatcgcag ttgagatcga tggctgtatt cacggcgtct
     6061 ccaataacgc aaaaaaggtg ggttcgtcaa ggatggaaat tcccagggct tgcgcctttt
     6121 ccagcttcga tcccggagaa gcgccagcga cgacgtaatg ggtatttttc gaaaccgatt
     6181 ccgttgtttt gccaccccgt gactcgatgc ggtgtttcgc ctcttccctg gtcatcccgg
     6241 tgagggtgcc ggtgatcacg aagatcttgc cggccaggct ccctaccgtc ggggaagaag
     6301 gggaatagag gctgtttacg ggggcgatgc cggcggcttc cagtttggcg agaaaacgtt
     6361 tgtttgccgg ttcccggaag aagcgggtga tgctcccggc ggcttccgga ccgagatcgc
     6421 ggatggccgt caattcatcg gcgtcggcgt cggctagctc cttcaatccg agatagtggc
     6481 gggccagggt cttggccatg ctttccccca cctggcggat gcccagggcg aagataagct
     6541 tttccagggg cggatttttc gaggcggcga tggccaggag gagatttccc accgattttt
     6601 cccccgtacg ctccagggcc agcagtttga cggcggtaag ggagtagatg tccgccgggt
     6661 ccttgatcag tgccgcatcc accagccggg agaccatttt cccccccagt ccttcgatgt
     6721 ccatgcctcc ccgggcggcg aaatggacga tccgctcctt gacctgggcg gggcaggcaa
     6781 tgttgatgca gcgatgggcc gcttctcccg ggaggcggac taccgcggtt ccgcataccg
     6841 ggcatgaggc gggcagtaaa aacgggatct cacgaccggt gcggcggccg ggaacgacct
     6901 tgacgacctc ggggatgaca tcgccagctc gctggacgac cacggtgtcc ccgaggcgga
     6961 tatctttccg gtcgatttcg tcttggttgt ggagactggc ccggctgacg gtgacgccgc
     7021 cgatccggac cggccgcatg atcgccacgg gggtgagggc ccccgttcgg cctacctgga
     7081 cgtcgatccg ttcgatgacc gtggtttcct gacgggcggg gaacttgcag gccagcgccc
     7141 agcggggtct ccgaaagatg gaccccaact ccgcctggag ggacagttcg ttgaccttga
     7201 gcaccatccc gtcgatttca tgattcagct cttcccgctg cgcgaggagg gagcgatatc
     7261 cctccagaca ggattcgatg ctttcggcta gtcgggcgaa ggggtgcacc tggaagcccc
     7321 aggctttcag ggcggcgagg acctcccact gggtcttcag ttccgccccc ccctccttga
     7381 tcccgacggc ataggcaaag aaatccaggg gacgccgggc ggtgatgcgg gaatccaatt
     7441 ggcgcaggga accggccgcg gcgttgcggg gattggcaaa cgacgcttcg cccgcctctt
     7501 ccctttcccg gttgaggcgc cggaaggccg cggtggcgat taccacctcc ccccggacct
     7561 cgaggcgggc ggggatttcg gtggtggcgc cgtcggcgag ggtcaaaggg acgccggcga
     7621 tggtgcggat attgtgggtg acatcttcgc cgacggcgcc gtccccccgg gtggccccgg
     7681 cgacgaggat gccgccttca taaactaggt tgaccgccac cccgtccagc ttgggttcga
     7741 ggacgtagct gatggtgttg ccgccggcga ggagacggtt atgaaaaacg cggatgtctg
     7801 cctcggtgat ggcgttggcc aggctgagca tgggcgaggg gtgggaaaag gccgtaaatt
     7861 tttccagggg cgccgctccc acgcgctggg tgggggaagc ggcggtgacc agttcgggat
     7921 aacgctcttc ccagtcccgg agttcgccga gaaggcggtc gtaagcagcg tcggagattt
     7981 ccggatcgtc gaggcggtaa tagcggcggt tgtggtaggc gatgagctcc cgcagttctt
     8041 cgatacgggc ggccgcggcg gcgatatccg gatcgtttcc cgccgacatc atgttttgtc
     8101 cggtttgatc agtttcaggg ccggtttttt ccgaccgacc gctcctgact tatggggcgg
     8161 gagaggcgtg ggggcaggtt gctgtttgcg gacctcttcg ttgaatatct ggtttttcgc
     8221 ccggaccgca ttgatgatga aaaagactat gacggcccgc tcccagtccc gggaggcatc
     8281 gaaatgctcc atccgttctt tgtacttctt ccacagggag gccagggagg cctcgtctaa
     8341 gccgagaatc ttttcggcaa tcctttccag ggtttttccc agcatgaccg tcctcttggt
     8401 gggttgaatt tgtcgttatc ctaaacgatc gtcggggaaa atgtcaacaa ccatatccaa
     8461 ataacacttg tatttttcgc ggtggtcatg ataatttccc acgtgttatg aaagccatcg
     8521 gtgccccctg gagaatgacc tatatccgcg gtgagaaggt aaaagaatgc gtcttctgtg
     8581 tcgatggggc aacctgcccc ggcggcgatc tacgcctcct ggaggggaag cgcgcatttg
     8641 tcatgatgaa ccgttaccct tacaccagcg gccatctcat ggtcattccc cgccgccatg
     8701 tcagcagcct ggaggagctc acggcggcgg agcgggcgga gatcttcgac ctggtcgcct
     8761 tgtcggtcca ggccctcaag gagagcgtcc gtcccgacgg tttcaacatc gggatgaacc
     8821 taggaaaggc ggcgggggcc ggcatcgatg atcatctcca tgtccatgtg gttccccggt
     8881 ggaacgggga cacgaatttt gtcagtgtcg tcggtgatgt ccgggtcatt cccgaggatg
     8941 tagcccagac ttgggagcac ttgcggccct atttcaagaa atcggcccgt tgagctgatt
     9001 taagtcgtaa atatcgccag gaggcttgat tatgaagatc gtctataccg tgattgtggt
     9061 ggtcctcatc atgtttatcg tcaccttctc cctcgccaac acatttccgg tccaattgcg
     9121 gtatctggat gtcatcaacg tcacccttcc cgcctatatg ttgatatttg tctgctttct
     9181 ggtcggggtc gtctttaccg gcttcatggg tatcgtcgag cgttttcgcc tcaaccggac
     9241 catctcccaa ctcaacaaga cgattcggga gctgcggcgg gaaataaggg ccaacgagcc
     9301 ccccccgatc atcgaagagc aaggcccccc cgcgccctga cggtcggtgc accgacggcg
     9361 gaatcatcga cataccgttt cttccccagg attggcccgg cagtgaagaa aaagatcatt
     9421 atcatcgcga tcatgctcgt cgtcgccgga cccatagtcc tggcggcggt cctgctcacc
     9481 ctcatgcagt ctccccgggc cgtaaacgcc ctggcggcgg ccctggagcc gatcaccggg
     9541 atacatctgc acgtccatga ccttgccttc gatcatcatc tccgaggtca tgtccagggc
     9601 ctccagatcc gcgccggtca cgagcggggc tttgcggttt ccctggccag ggcggagatc
     9661 gacggcaatc ttgaccttgg cttcaagctc cgggcggaga ggatcgttct ggaaggtcca
     9721 aaattcacct ttcacctgaa ggacgaggaa tcggaatccg accccttcgc cgtgctcggc
     9781 aagatccccc cggtcaagca gttggtcgtc aaggacgggc acctggaatt gaaggccccc
     9841 ggagcagtct attccatccc gggcctggaa atgacgatta acgactttga tccgacggga
     9901 cacggtaagc tttcgggccg gagcagcttc catatccaag ccggagatat ggatacccgg
     9961 ggtgtgggcg aggccgccct ggaatttcac cgtttttctc ctcgtccgtc cgctttcggt
    10021 tctctgcggt tttcccttgc cgacggcact tacggcaaca tgaagcttac cggggccgta
    10081 ctggcgtcgg cgatcaggct ggatgacgga ctcctcaccc tgaataacgt tcatgccgcg
    10141 gtggaagaac tggcccggag ggaaggtccg tcgccagcta tcatcaggaa tctgcaagcg
    10201 gatctcaatg cttcttacga tcagcgcacc tcccggtttg cgatcaccaa cggcaaggtg
    10261 tacggagcga gtctgggcgc tctgaacgga cgtatctcag gaacaatcaa gccgctttcc
    10321 tggcatgcct ccctccaagc cccggtgctg gatctggccg gggtcttcac cctggccaga
    10381 cctctgctcc ccgcggaata ccgcggctgg aactgtaaag gccgcggcgg actagaggtg
    10441 gaaagccggg gtaaaaccca ggacgggacg acggtttggg aggccgacgt tgctgtggat
    10501 ttccgggaag ggggatttgc ctcggcggac gggacgaaag ctggggaacg gatttccggg
    10561 cgggtcgaac ttaagctgga agcgccggaa aaaggacgcc ccggaaattt tcgcttctcc
    10621 atggaaagcg gtggcggcga actgctctgg gcgtcatact acaatgactt ccgtggggaa
    10681 aaggtgaaga tcgcctcccg gggagatttt gccccaaatc ccttttacct tgccgctgac
    10741 ggaacctgcg atatcttcca gagcggtgac tatagttttt ccgcccggtt ttcccccgag
    10801 caaaacatcc tttcccttcg ggcccggggt atatcgcttc cccgcctttt ttccacgttg
    10861 gcgcggaatt tcctccagca gaactatccg gagcttcagg acctgcgagt ggaaggagag
    10921 gcggatctgg aattgacggt tgtggcggca ccggaacaaa aaaccatcgc cggtgaattc
    10981 gacctccacg gcggatcgct ccaatcgcct ttccaccggc ttgtcctggc cgggatcgag
    11041 atatccctgc cctacgatct cgcgctggct ggaaaggcgg tcactccgcc cccggcggat
    11101 ctccggcggg gatcgatggc tttttccgga ttcgagaagg gagatatccg aataaagggt
    11161 ttcgccacgc cggtcatcct ttccggcaac cggtttaccc tgccggaacc ggttacagtg
    11221 ccgattttcg gtggggtggt ccgcctggcg ggtttgcagg ccgaggatct tttgcggccg
    11281 gaaatgcggt taaagacgtt tttggagatc catggaatcg accttgagga attgacgggc
    11341 ccggcgctgc ccattcctct ttccggggtc atcaatggag gcttttcccc cctggtcttt
    11401 cagaaagatc tctggaccgc cagcggggtg attgtcgtgg agacgttcgg cgggcggatt
    11461 gccgtggata atctttttgg cggccgtctc ctctctaaag cccgtttttt cggtggagac
    11521 gccggattcg aaagcatcga cctggaggcg cttacggcca gcatcgagat cggacggatg
    11581 accggactca tccggggagg gctgaacaac ttccgaatgg aatacggaca gccctcccgt
    11641 ttcgaactgg tcatcttgtc cgatgagagc cgtaatatgc cccggcgaat aagcgtggac
    11701 gccatcgaga acctctccat catcagcacc ggttccgggg cggtttcctc gctgatgagc
    11761 agcggcctga acatgttttt caaagactac ccctatagtc ggatcggcat ccgctgtgcc
    11821 ctggcggacg atatattttc cctccagggg ctcattcacg aaggggggac ggaatatctc
    11881 gtgcgccggg catggctccg gggtattgac atcatcaacc agaatccgga taattccatc
    11941 agcttcaagg acatggcgga acgggtggga cgcatccgcc agccccggca ggaggagaaa
    12001 ggggtgtcgt aggcgcccgg atttgttatg agtgcggatc aattttagca gaggaggacc
    12061 atatgagagc gatatgtaaa gtttgcggct gtctggcgat tttgttcgtt gcggcctgtg
    12121 tgacggtcaa tgtctatttc cccgccgcgg aggtgcagaa ggccgctgac aagatcgtgg
    12181 acgacgtccg taagaagacc ggcgaaaccc cggcggcgcc gtcgagcggc gtggagatat
    12241 ggaaacgttt cgggtttggc gtccagccgg cctacgcgca gatgaatatc gacatttcca
    12301 cccccgccat ccggggcctc aaagacgcca tgaaaaacag atttccccag ttgaagcctc
    12361 tttatgatca gggcgccgtg ggggagaaca acaacgggtt gatcgaactc cgggatgccg
    12421 ccgctttgag tctggcacag cgcagtcagg ccaaccggct tatcgatcag gaaaacagtg
    12481 accggaacgc cctgtacggg gaaatcgccc aggcaaacaa actcggtccg gagtcggtgg
    12541 cgcagatcag gaagatcttt gccaacagtt ggcgggaaaa gtcactggcc ggctggtggg
    12601 tgcaaaacga ccgcggcgtc tgggagaaga agtagcggcg cgcgggtggc taagatcgct
    12661 ccgggccctt tattgacgac atcgtaaaaa tccatatgcg gcgttccgct tcagcattcg
    12721 tcactgcgac gtatgcccaa gtacttctca ttcctcagga ttcgtgcgcc ttacctctgg
    12781 atttttttac ttcgtcttac gagttcatct tgattaaatg gcgcagtccg cgtcgctgcc
    12841 ctgaatcttg gccacggcgt gggaaagggc ggggaggatt acggccagat tttcccggac
    12901 cccccgggga cttccgggca gattaacgat taaggtccgg ccgcggatcc ccgccagcgc
    12961 ccgggagagc atggcgtgag gggtaacttc caggccggcc cggcgcatcg cctcgcccat
    13021 gccgggtatt tcccgttcca ggacggcgcg ggtggcctcc ggggtgacgt cccgggggga
    13081 gacgccggtg ccgccggtgg tgacgataat atccgcctcc tggcggtccg ccagctcgat
    13141 gaggatttcc tttatctctt ccacctcgtc gggaacgatc tggatggaga gcacctcgat
    13201 ctcagcggtg gcgaggatcc gggcagcttc cggcccgctc agatcctccc tttgcccctg
    13261 cgatcccttg tcgcttaccg tcacaatcgc tgctctgatc aatctatttc tccgcctctt
    13321 ccttcttttt cgactcggcg atgatcttct cggcgatatg ggccggtacc tcctcatagt
    13381 gggaatgctc gtaggtgaag gtgccccggc cgctggtcat ggaacggaga tcgggtgcat
    13441 atttgaggat ctccgccagg ggcacctgac ccttgatgat ctggatggaa cccttggtgt
    13501 ccatgccctg cacgcggccc cggcggccgt tgagatcgcc gatgacgtcg cccatatact
    13561 catcggggat ctctatgaag atgttgacga tcggttccag cagcacgggc tggcaggcca
    13621 gcacgccctt cttgaagccc atggagccgg cgatcatgaa ggccatttct gaagagtcga
    13681 cggtgtggta ggtgccgtcc accagggata ccttgaaatc caccacgggg tagccggcca
    13741 cgacgccttc ctccatcgcc ttgacgatac ccttttccac tgccgggcgg tactgctggg
    13801 gaatgacgcc gcccacgatc ttgtcctgga attcaaaacc cgtgccccgg gggaggggct
    13861 cgatatcgag ccagcagtcc ccgtactggc cgcggccgcc ggactgcttc ttgtacttgc
    13921 cctggacgtt cgtcttgccc ttgatggtct ccttgtaagg aaccttgggg gttttcaggt
    13981 tgacctcaac gccgaacttc cgccgcatct tttccacggc gacctcgatg tggatctgtc
    14041 ccatcccgga tatgatcatc tccttggtct ggtcgtcccg gtggaaatgc atggtcgggt
    14101 cttcttcgat gaggcggttg atggaggaga cgatcttgtc ttcgtcgccc cggctcttgg
    14161 gctcgatggc aaaggacatg atcgtgggcg gcggcgtcac cttttcatag atgatcggat
    14221 tcttttcagc gcagatggtg tcgccggtgc cggtctcctt gagtttggcg aaggcggcga
    14281 tgtccccggg aatcaccatg tccgtgggct tctgggtctt gccttcgaga aagaagatat
    14341 tgccgaagcg ttcggagagc ttccgggagg cgttgtacat ccccgcctct ggggtgagaa
    14401 cgccggaata gacccggaag agggtaagcc ggccggcata gggatcggcg atggtcttga
    14461 acaccaaagc ggagaagggg gcgctttctt cggggaggcg tgtttcctcc tctccgccgt
    14521 cgggcttctt tccctttacc ggcccgcggt ccacgggcga gggcagatac cggacaatca
    14581 tgtccatcag cagacccgtg ccaatattct tcagggcgga gccgcaggcc acggggatga
    14641 tcgccccgct gtgcaccccg gatttgaggc cgtcacgcag ttcttcatcg gtcaactcgc
    14701 cggcgtcgag atatttgtcc atcatcgcct cgtcggattc ggcgatgtct tccaccatgg
    14761 tttcccgcca tttctgcgcc tcttccgtca tatcggcggg aatgtcggtg gtcttgaagg
    14821 tccccttcaa gtcgtcatag atcagcgcct gcatggtcat cagatcgact attccccgaa
    14881 aggaatcatg ggccccgatg ggaaggcaga tgggcgttat cttgacgtcc atcttttcct
    14941 tgatgctggc cacggcggtc tggaaatcag cccgttcccg atccatgcgg ttgatgaaga
    15001 ccatgcggga aagatggtat aaatcggtat actcccacac cttctgggtc tgaaattcta
    15061 ccccccccac ggcgtcgatc actacgatgg ccgcgtcgcc gatccggaga cagctctggg
    15121 tatcaaagga gaagttggaa tccccggggg tgtcgatgat gttgatcttg catttctgcc
    15181 attctacgga agtggtagcg gcgcttatgg aaatccgctt tttttgttct tccggttcat
    15241 aatccaaact ggaggttccc tcgtcaaccc gtccgaggcg atccgttttc ccggatacat
    15301 ataggcagga ttcacccagg gatgtctttc ccgtggatcc gtgtccgacg attacgatgt
    15361 ttcttaatga cttggagtca tatttggcca tgacttctcc tttctatggc gcaatggtag
    15421 gattggcact gtgatcggcc tgtttagctt aacaattccc gcgatgtcaa gacaattttc
    15481 gtttacaata atgatgttga cagaaattcc ggcaataagt ataaaggccg acttatatcg
    15541 ataaatgtaa ctcctttcaa gggaaggtaa tccgatgaag aagagaaaca ggaagatcag
    15601 taaagtgctc attgccaatc gcggcgagat tgccctgcgg atcatgcgca cggtccagga
    15661 gatgtccctg gatgtcgtgg tcgtttacga acatgccgat gcggaagcct attatgtccg
    15721 ccaggccaac gaagctgtca tgatcggcga gggtccccgc aaggattacc ttgacatcga
    15781 caagatcatc tgggcggccc ggaagaccgg cgccgacgcc attcaccccg gctatggatt
    15841 tctggccgaa aacccggaat tttccgccgc ctgcgaaaag gcggggatta ttttcatcgg
    15901 tccgccgccc caagtcatcc gcgatctggg caacaaggtc gtggcccgga acatcatgga
    15961 gcgcgcgggg atacccttca ttcccggcac cgccgacatc ctcaagggag gcgacggggt
    16021 gaacgaggcc ttttccttcg cccgcgcctg tggctatccc gttatgctca aggcctcctg
    16081 tggtggcggc ggtcggggga tccgcaaggt caaggatgag gcggatctca tggtgcagct
    16141 tcccctggcc cgttccgaat ccctgtcggc cttcaacgac gaccgcatct acatggaaaa
    16201 atgcatcgag tgcccccggc atgtggagat ccagatcctc gcggacaagc acggcaatat
    16261 cattcatctg ggctcccgag actgttcgat tcagcggcgg caccagaagc tcctggaaat
    16321 cgccccggcg gaccttcccg aggatgtcct ggaggcaatg tacgaatgcg ccgttagggc
    16381 ggcccgggaa gcgggttaca taaacgccgg taccgtggaa tttctggtgg atgctcggac
    16441 caacgagttc tggttcatgg agatgaacac ccgcctccag gtagagcata ccgttaccga
    16501 agagttgacg gggatcgaca tcgtccggga gcagatccgc atcgccgaag gcgcggtcct
    16561 gaatatccct aaggaacgta ttcacctcca cggcaaggca gtgcaggtcc gcatcaatgc
    16621 ggaagatccc aagaacaact tcatgcccga gggagggaaa cgggtggagg tctatcagtc
    16681 cccgggaggt ccgggggtcc ggctggacgg ggccgtatat cagggatacc gtatccccac
    16741 ggattacgac tccctcctcg taaagatgac cataaggggg tacaactggg agcagacgct
    16801 ccagcgcttg aagcgttccc tccgcggatt tatcatcgtc ggtcccaaga cgaccattcc
    16861 cttctatctg gccatctgcg acgataagga tttccgggcc ggcaaattcg acacgggtta
    16921 tatcgacacc catccggaga tattcgaata tccggaggcg gaacgggaaa tcgccaaact
    16981 gggcaagctc atcgccgaga ttcacgcccg taagatcaac cggcatgcct actaatatag
    17041 atattcaaga gaacgagaac atgaaaaaga taaggaaaaa agaactatac cgggaaggcg
    17101 aaccgcgcat caccccccgg gaaatgagag ataagggcgt gaaatacgtc ctggacaaga
    17161 taaaaaggca gccgggctat tatgtcacca acacggagcg ggacctttcc cagtctgatt
    17221 tcaagaaccg gatcatgccc cacacccaac tgctcgttgc cgaggagcgg aaccaggccg
    17281 gcttcttctc cttggagatt accgggggcg cctccgtcca tgtggacatg ctgcgcaagc
    17341 agatcaaccc cctggagaaa ctacaggttt taggagagcg gatgccggac acgatgttcc
    17401 aaaccctctg ccgggggatc aatctcttcg gttaccgacc ctatcccgac aatgtgatcc
    17461 ggatgacggt gcgcaccttc gcccgttatg tccacgtctg gagagtattc gattttctca
    17521 accatatacc caacatggtg ccggtgttcg aggaggtgaa ggcggcgggc tgcattctgg
    17581 aaccttcgat ctgcttctcc acgggtccgg agcacacgga cgcgtactat gtccgcaagg
    17641 tgggagagat cctggacgtt acgggggaag acatcctgct gtgcatcaag aatcatggcg
    17701 gcctggggac ctcgaaacgg atcggtgatc tggtccgggc catccatgac cggtatcccg
    17761 acctggttat ccattatcac gggcacaaca ccgacggcga agacatcggt cggatagtcg
    17821 cggcggtgct caacggtgcg aagatcgtgg acgccagcga tcacgccttt acgggcttct
    17881 atgggcctcc cccgcttttg tccgtggtgg acatcctggc ggattatggc tatcaggccg
    17941 cggggctgga ccgtcaggcc gtcatcgaaa cctccaacaa gctccgtccg gagcgggaat
    18001 attaccggga tttcgaatcc cagttcctcg gcttcgatcc caccgtccag atccacaagc
    18061 tccccggggg cgccaccggt tccagcttcg agcaggccgt caagggcggt ttcctccaaa
    18121 agatgcccga gatcctccag aaggagcttc cccgggtgca ggtggaactg ggcaactggt
    18181 ggagcgtgac gccggggtca cagatcctct ggacgacggc ggtgaacaac gtcctgaagg
    18241 gggaacgcta caaggatgcc aatgacgatc tgaagaacct catgctgggc cgctacggcg
    18301 atcttccctt ttacatgccc tccgacgagg tctaccgcac cgtcttcggt ccggactgga
    18361 agcagatcgt ggaatgcgac acatgctacc agaagatcga ggacgtggac ctggaggtgg
    18421 agaggaaggt cctgcagcac cgcctgggaa gaaccgcgac ggacgacgag ttgacgctct
    18481 atctccagca tcccaacgac gcggtggact tcttcaagtt cgaggcgact tacggcaaga
    18541 cctgggtgct ttctccgcgg atctggtttc gcaagggcgg tttcagcctg ggggagaagt
    18601 ttgacatccc cgatgcggag ggcaagcttc acagcgtaga aatcggtccc caacgcaaga
    18661 cgaaatcagg ggatacggcg acctatctga tcattgatca tcaccccgaa cccatcgtta
    18721 cggaacagga ggaggaagag gggagcaaaa agcagaaaaa gaccatcatg tcccccaagg
    18781 agatcgacgc ggcctggaag gccggggaca tccgttccca tctcactggg acggtgaacg
    18841 acatctccgt ggagcagggt gaagaggtgg cccagggaca ggtcctcatc gtcctggagg
    18901 cgatgaagat gctcaacaac atcaccgcgg agataagtgg caaggtatcg gagatcaatg
    18961 tttccccggg agacaaggtg acgatcggtg acaagctgct gtttgtggcc aaagactgaa
    19021 acggaacaaa tgaatattga tcaggcgggt tggcggaacg gtgaaaacgt tgcgttccgg
    19081 tgctcgccgt gagtgaaaat tccggcggga tagggccgga atttttcttt taaaggtaaa
    19141 aaacatgatt ggggctttac tgaaaaagat catcgggacc aaaaacgatc ggatcataaa
    19201 ggaaatatcc gttgtcctcg atgagataaa ccgtctcgag cccgccatgg cgtcccggac
    19261 cgacgccgaa ctccgggcca agaccccgga gtttaaagag cggttgctga agggttcttc
    19321 tctggatgat ttactccccg aggccttcgc cgtggttcgg gaggccgccc ggcgaaccgt
    19381 gcagatgcga cccttcgacg tccagatcat cggcggaatc gtcctccatc agggcaagat
    19441 cgccgagatg aaaaccggtg agggcaagac cctggcggcg acactgccgc tttatctcaa
    19501 cgccctgacg ggcaaaggct gtcatctggt cacggtcaac gactacctgg cccgccggga
    19561 cgccggctgg atgggtccga tatataattt tctcggtcta tccgtcgggg tgatcgttca
    19621 cggcatggaa gacgaagaac ggcgccaggc ttatgccgcc gacatcacct acgggaccaa
    19681 caacgagttc ggcttcgact atctacggga caacatgaaa ttctccctgg aggattatgt
    19741 acagcgtgat tttaactatg ccatcgtgga cgaagtggac agcatcctca tcgacgaggc
    19801 ccggaccccg ctcatcattt ccggcccctc cgaggaatcc accgataaat actaccgaat
    19861 caaccaggtc attcccaggc ttcgcaagga cacggactat gccatcgaag agaaaaccag
    19921 gacggtggtc ctaaccgaag aggggatcgc ccgggtggag aagtacttga aggtgcaaaa
    19981 cctttacgag cccaaaaaca tcgagcttct ccatcatgtt aatcaggccc tgaaggccca
    20041 tacgatcttc aaacgggatg tggattatgt cgtcaaggac ggccaggtca ttattgtcga
    20101 cgagttcacg gggcggatca tgcccggccg gcgctatagc gacggtctcc accaggccct
    20161 ggaggccaag gagcacgtga agatcgagcg ggaaaaccag accctcgcct cggtcacctt
    20221 ccagaattat ttccggatgt acgataaact ggctgggatg accggtacgg cggataccga
    20281 ggcggaggag ttcaaaaaga tctacaaact ggatgtcgtc gttatcccca ccaacatgcc
    20341 catgatccgt accgatcaca acgacgtgat ctacaagaca gaaaaggaga aattccacgc
    20401 cgtcatcgag gagatcaagg agatgcaccg tcagggcagg ccggtgcttg tgggaacgat
    20461 ttccatagtc aaatcggagc tcttgagcgc tatgctcacc aggacgggga tcaaacacca
    20521 tgtcctcaat gccaaacatc atgagcggga ggcagagata gttgcccagg ccggacaacg
    20581 gagcgcggtg accatctcca cgaacatggc gggacgcggt acggatatca agcttggaga
    20641 aggcgtcgcc gatctggggg gacttcatat ccttggcacg gagcgtcacg agagccgccg
    20701 aatcgacaat cagctccggg gccggtcggg gcgccagggg gatatgggtt catcccgttt
    20761 ttatctgtct ctggaggacg acctcctccg gatcttcggg gcggaaaaga tctccgccat
    20821 catggaccgc atcggcatcg aggagaacga gcccattgaa aaccgcttgg tttccaaggc
    20881 catcgagaac gcccagaaac gcgtggaagg ccagaacttc gacatcagga agcatcttct
    20941 ggaattcgac gacgtgatga acaagcagcg ccaggttatc tatgagcaac gccgtaaggt
    21001 cttgagcggt caggatctct ggacggacat cgaggggatg gtcgaggaga cggtcgccga
    21061 catcgtgccg gattatgtag acgaaaaggg ccatcccgag gattgggacc tcatgtctct
    21121 ggatgaccgc atataccagc ggttctccct gcgcctcaat ctccacgaga agaaggggga
    21181 gatgagcgcc ccggagatcg aaggggcgat caccgccgcc gtcaactccc atctccgccg
    21241 caaggaggaa aacttcggcg ttccgttgat gcagtacatc atgaccatga tcatgctgca
    21301 gagcattgac gcccagtgga aggatcacct cctcggcatg gatcatctta aagagggcat
    21361 cggccttcgg gggtacgggc agaaggatcc tgtccgggag taccagaaag agggctatga
    21421 tatgtttatg gccatgatcc agcggatcaa ggaggatacc ctggaaaagc tctgtctggt
    21481 ccagatccgc cgggaggaag atatcgaaga gatacagcga caggcggaag atgattacat
    21541 cctcagccgg ggcgaagacg tgagtgcgac gccggtgaaa agggagaagg agaaggtggg
    21601 ccgcaatgat ccctgcccct gcggcagcgg caagaaatac aagaagtgtt gcggcagata
    21661 gacggtgcaa ggataagatc atgaaggaac atgcaatggc agtgcccggc tttctcgcca
    21721 gtggcgtcag cgccgggatc aaggaaacgg gggcgaagga cctgtcgctg atctattcgg
    21781 aaacgcccgc ccgggccgcc ggggttttta ccaccaacgc ctttccggcg gcgccggtgg
    21841 tcttatcgcg gcagcggatc cgctgcggat acgcccaggc catcctcacc aacagcggca
    21901 atgccaatgc cgccacggga gaggatgggt atcgcgatgc cctggccatg ggcggcgctc
    21961 ttgcacggcg cttgaaaatc gataaagatt tcgttctggt atcctccacc ggggtgatcg
    22021 gccaccgcct gcccctggac aagatccgaa aaggcatacc gaagcttgtc gcgggattgc
    22081 atcccgaggg gataaagaat gccgaggagg ccatcatgac cacggaccgt tttcccaagc
    22141 tgtccttccg gaaacggatg atcggcggcc gggaaatttc cgtctgcggc atggccaagg
    22201 gagccgggat gattcagccc cacatggcca ccatgctcgc ctatgtcatg actgatgccg
    22261 atattgacgc caaggccctg gaccgggcgt ttcgatacgg tgtggagcgc agtttcaatg
    22321 ccatctccgt ggacggctgc atgagcacca acgatacggc gctggttttg gccaacggcg
    22381 tggcgggaaa ccggcggata gaagagcggg gccgtggctt cattgccttc cgtacgatgc
    22441 tggccgaggt catgactgaa cttgccaggg atatggttcg ggatggcgag ggggctacca
    22501 agctgctgga aatcactgtg gaaggggcgc gaacggcggg ggaggccagg aagatggctt
    22561 atgccatcgc caattccaat ctggtcaaga cggcctttta cggcggcgat cccaactggg
    22621 ggcggatcat cgccgcggcc ggggcgatgg gcgtgccgct gcccctggaa cgcgtggagc
    22681 tgcttttcga cggcgtttcc ctgtttagcc gggggctgaa aacagccgga aacgaagagc
    22741 tgttaaaaga agtaatgagc cgggcggaga taaaggttat tttccgggcg ggaatgggga
    22801 acaagtcctt ccgggttttc gcttccgatc tgggcttcga gtacatcaag atcaacgccc
    22861 attaccatac ctgagcgtcc tcatcgcagg tcaagataaa tatattgaat gggtgggaaa
    22921 atgggcttgc gccttaaccg gtaatatgat aatgctaccc gaatttcaca cattcccgga
    22981 tgggcttttt gttgcttcgt catccaagga gaagtcctaa gcccgcggga agggaatggc
    23041 ggaaaaaaac aacgaaggag tatttgtatg gcacacattt ccatgaagct gctgctggag
    23101 gcgggggtcc attttggtca tcagaccaac aaatggaatc ccaaaatgaa gccctatatc
    23161 tttggggcaa gaaacggtat ttacatcatc gatctgcaac agaccgtcgt cctcttccat
    23221 aaggcctacg aattcgtggt ggacatggtg gcgaacggtg atgagctgct gttcgtgggt
    23281 acgaaaaagc agtcccagga gtccatccgg gaggaggcgg gccgttgcag catgccctat
    23341 gtgaaccagc gctggctggg aggaatgctc accaatttca ttaccatcaa aaaaagcatc
    23401 gaccgactga acaccctgga taaaatgatc gaagacgact cgatccgggc ctttccgaaa
    23461 aaagagatcc tctccttgca gaaggaaaag gcaaaactcg aaaagatcct cggcggtatc
    23521 cgcaagatca aagggcgtcc cggcggcctt ttcatcgtgg accccaagcg ggagagcatc
    23581 acggtcaagg aggcgaggaa actagggatc cccatcgtgg ccatggtgga caccaactgc
    23641 aatcccgatg atatcgatta tataatcccg ggtaacgacg acgccatccg ggcgatcaag
    23701 ctcttttcag ccaagatcgc cgatgccgtg ctggaaggga agaagaagtt cgaggaacgg
    23761 atccaggccg aaagcgacaa ggagggaccg gtccgggagg tggctatcga caatgaggaa
    23821 gaaaacgatg tccccgaaag catcgagacc aaggatagtg tctccgtagc cgctcatgct
    23881 gaggaatgat gacggacgtg cgggatttcc tgaagaataa cgaggaggta aaaagtgaat
    23941 attactgtgg aaatggttaa aaccttgaga aataaaacca acgccgggat gatggagtgt
    24001 aaagaggcgc tgaaggcggc ggacggcgat tttgaaaagg ctgtcgatta tctgcgaaaa
    24061 aaggggatgt cggcggcgac caagcggtct tccaaggccg ccaaggacgg cacaatcgcc
    24121 cattacatcc acatgggcgg aaagatcggc gtcctggtgg agttgaactg cgagaccgac
    24181 tttgtcgcta agacagaggc ctttcaaact atggcccggg atctggccat gcatgtggcc
    24241 gcctcgaatc ccctctacgt ccggccgcag gaaatttccg ccgacgccct ggagcgggag
    24301 aaggagatct acaaaagcca gcttctgacg gagaaaaagc cggaaaagat ctgggacaag
    24361 atcatcgagg gcaaactgag aaaatactac gaggaggttt gtctcctcca gcaaaaattc
    24421 atcaaaaata ccgatattac tgtcgaaacc ctggtcaacg aaatgattgc caagaccggc
    24481 gaaaacatca tcgtgcggcg ttttgcccgg ttccagctcg gggaagaaat caaatcctga
    24541 tggtgaaaaa ggccgtttat cgacgggtgt tgctgaaact cagtggcgag gccctggagg
    24601 gggagcgatc cttcggcatc gatttcgatg tcgtcaacga gatagcccaa gagatccggg
    24661 cggtggtgga actcggcgtt cagttggggg ttgtcatcgg cggcggtaac atctggcggg
    24721 gcggagaggc cgcaaagcgg gggatggaga ggtcggcggc agactacacg gggatgttgg
    24781 ccaccttgat caactgcttg gccatgcagc atgccctcga acatcttggc gtcgccacac
    24841 gcctccaatc cgccctggag gttcgcgaga tagccgaacc ctatatccac cgccgggcga
    24901 ttcgccatct ggaaaaaggc cgggtggtca tcttcgccgg tgggacgggg aatccgttct
    24961 ttaccaccga tacggcggcg tcgctccggg ccgtggagat ccgggcggaa gtgatcatga
    25021 aggccaccaa ggtggatggc gtctatgacc gcgatccggt gggtaatccc gatgcggtaa
    25081 tgtataaaat aataacatat aacgaagtct tggcaaaaaa ccttaaagta atggatgcaa
    25141 ctgcgatttc cctgtgtcgg gaaaacggcc tgcccattat cgtctttaac ctgagaaaaa
    25201 aggggaacat cgccgccgca atctgcggag gaaagcttgg cacgctcgtg gagggttaga
    25261 ttcatgaaag aagtcgtata cgaggaactg aaagataata tggagaagat cctggggtcc
    25321 ctggagaagg gcttcagtaa ggtgcgaacg ggaagggcgt cactggcgct tctcgacggc
    25381 atcaaagttg attattacgg cgtccccacg ccgctgaatc aggtggcgag cctctcggtt
    25441 cccgaaagcc gcctgatcgt catttcgccc tgggataccg gggtcatcgg ggcgatcgag
    25501 aaggccatcc agaagtcgga actggggctt atgccctcca acgacggcaa gatcatccgc
    25561 ctgcccattc ctcccctgac ggaggaacgg cgcaaggatc tggtcaagat cgtcaagaag
    25621 ctggcggaag agggcaaggt gaagatgcgg aatgcccggc gggatgccaa cgaacagttc
    25681 aagtccctga agaaggaaaa caagatttcg gaagacgaga tgtttaccgc ccaggatgaa
    25741 gtccaaaaac gaatggacaa gtatatcgag aagctcgaag ccgtactggc ggtcaaggaa
    25801 agcgaaatta tggagatttg atgaatcgct gacctgacgg ctgatgggaa gatcttttgg
    25861 ggagtcggca aactccccat ttttttaaag tctacttgcc aagagcccgg cgatatgtta
    25921 cgcgaaaaca tgtccatcac gtccatttca caccatcagc gcgccgcatt aactaccggc
    25981 gccggcgtga atctccgccg ccgatcccga ttcatcgtaa gtcccaactc cggcgtgtct
    26041 ccggcgaaga tgccagggga gcggtaatgg cggatatcga cttgcagaaa cttccccggc
    26101 acattgccat catcatggat ggaaacggcc gctgggcgaa acaacatgcc ctgggaagga
    26161 tcatgggcca tcagaagggg gtggaggccg tccggaaaac cgtccggtta tgcagggaaa
    26221 taggcattca atatctgacc ctgtatgcat tttccgtgga aaactggttg cgtccggatc
    26281 aggaagtggc ggcgctgatg cagatcctca ggcagtatct tcgttccgaa ttggcggaga
    26341 tgatggagaa tgacatccgc ctgcacgcca tcggcgacat cgacggtctc caggcgccgg
    26401 tacgtaaact gctggccgaa accatggccc ggacggcggg gaaccggggc atggtcctga
    26461 acctggctct cagttatggc ggacaggatg aaattatccg ggcggtccgg cggatcgccg
    26521 aagacgttcg ggagggaacg ctggccgccg aagacatcac ccgggaatcc tttgctgcct
    26581 atctatatac cgccggcatc cccgatcccg atctgcttat ccggaccagc ggcgagtttc
    26641 ggatctccaa ttttctcctc tggcagtcgg cttatacgga gttctatttt accgaagtcc
    26701 tctggccgga tttcgacaag aaggatctct tcgcggccat tgcggattat cagcgccggg
    26761 agaggagata cggcctgacc agcgaacagc tccagaaggg agcttcctcg tgaattccca
    26821 tataaaacgg tggcttaccg ccatagtcgt ggtccccgtc ctgtttgcgg taatcgcctg
    26881 gggtggtgaa acgggttttt ttgccctcat cgtggcggta atccttctgg gcattcatga
    26941 atacagcaac atggtcctgg atgcgtccca ggtaaacgaa aagattcaag ccctggttat
    27001 ctcggcgctg attcccgttg tattcttcct gggctcgccg gagatgctga caggtcttct
    27061 atgcgcggcc atgctcactg tattcatcct gtatcttgcc cggatcaaaa aggaaaactt
    27121 cgacctgacg ccggtgatga agataatctt cggcgtcttt tatgtttcct tcctcctttc
    27181 tcatctgatc tggctgcgcc ggggggagtc aggggtgctg tggatcttct tcgtcatcgt
    27241 cctggccttt tccggggatg tggccgccta ttacgtggga cgcacctggg ggcgacggaa
    27301 actgatgccc ctggtcagcg ccggaaagac cgtcgagggg gtgttgggtc ttgtcgccgg
    27361 aagcgcgctg ggatgcctga tcttcagttg gttcttcctg ccccagctct ttccctggca
    27421 tgccatcgtc ctgggggtct tcgcgagcgt cctggggcag cttggcgacc tgtgcgaatc
    27481 ggtgctgaaa cgcgccgcgg gggtcaagga ttcggggagc ctgctgcccg gtcacggcgg
    27541 tatcctggac cgcctcgatt gcctcctttt tatcgtcccc tgcgtgcatt actatcgcta
    27601 ttattttatc tcatgaagaa tatcgccatt ttaggctcca ccggttctat aggcacgcag
    27661 gcgctggcgg tcatcgactg caatcgcgac cgcttccgtg taaccgccct ggcggcaggg
    27721 aggaatgtcg tcctgctacg ggagcagatc gctaagttcc ggccccggat cgtcgccgtt
    27781 ttcgacgagg aatgcgcccg gcggctcgga agcgagccgg gactaccccg ggaattgaag
    27841 attctgtccg gacccgatgg atataacgaa attgccgccc tgccggaaaa cgatctggtg
    27901 ctctcggcca tggtgggggc ggccggactt gtccctaccg tgaccgccgt ggaggcgggc
    27961 aaggatgtag cattggccaa taaggagaca ctggttatgg ccggggcgct ggtcatggaa
    28021 aaagcggcgg cgaaaggggt gcatatcctg cccgtggaca gcgaacacag tgcgattttc
    28081 cagtgcctcc agggaggccg cgccggggag gtccgaagga ttatcctcac cgcttccgga
    28141 ggcccctttc gtacctggat cagggaggaa ttacgacgcg tgcggccaga acaggccctc
    28201 cggcacccca actggacgat gggcaggaag atcaccatcg attcggccac catgatgaac
    28261 aaagggctgg aaataattga ggcccggtgg ctcttcgcag tgaaagtgga acaaatcgat
    28321 gtggtcgtcc acccccagag cgttgttcac tccctggtgg aattttgcga cggatcggtg
    28381 cttgcccagt tggggcagcc ggatatgcgc ctgcccatcg cctatgcctt ttcctggccc
    28441 gagagactgg ccggtaccgg cgcgtttctc gatttccgga gcattccggc cctggagttc
    28501 cacccccctg acgccgaccg gtttccttgc ctgtcattgg ccggcgacgc cctccggatc
    28561 ggcggaacca tgccggccgt gctcaatggg gccaatgaaa tcgcggttca ggccttcctg
    28621 gaagaaagga tcctctttac cgatatcccc gaagttatcg ccggtgtcat gaaatcgcac
    28681 cgagtcgtct tcgcgcccac gcttcgggac atcatggccg ccgacagcga ggctaggaaa
    28741 cgggccgtca aaatgatcga ggagaaaaaa gattggttgg cataagtatc ttatcggtta
    28801 tcgtgctttt ggggatattg atctttgtcc atgaactggg acatttcctc atggcgaaat
    28861 atttcgacgt cggggtccag aaattttccc tgggcttcgg ccgaaagctc attggcaaaa
    28921 agatcgggga aacggaatac ctcctgtccc tggtacctct gggcggttat gtgaagctcc
    28981 ttggggaagg ggagggggaa gagctgtcgg cggaggagga acggcgctct ttcagtcgtc
    29041 aatcggtgtg gaagcggatc accattgtct tcgccggtcc gctattcaat ttcatgctgg
    29101 cgattctcat ctttaccggg gtttacatgc tcggggtgcc cgttctgacc accaccgtcg
    29161 gcggcgtcca ggagggatcg gcggcttatg aggcggggat gaagcccggc gatgtcgttc
    29221 aggccattga tggggatgcg gtcgggcgct gggacaggat ggcggtgaag ataaccgaga
    29281 gcgccggaaa ggagatgagg ttcaccgtcc tccggcaggg agtgccactg gagttgcaga
    29341 tcgttcctcg tgaaatgaaa gggaagaaca ttttcgggga ggaaacgacc tctttcaaga
    29401 tcggggtatc cccggccagc ctgacggtca tcgagcgcca aaatccagct ctggcaatgt
    29461 ggaccgccct gaagcagacc tggttcatta ccaagctcac cttgatcagc atcgtcaaga
    29521 tcctggaggg ggtaatctcc ccccggaccc tgggcggtcc gatcctcatc gcccagatgg
    29581 ccggcgccca ggtcaaggaa ggcctcatac ctttcgtcct gttcatggcc ctcctgagca
    29641 tcaatctcgg ggtgctcaat ctcctcccca ttcccgttct cgacgggggc catttgcttt
    29701 tttacttcgt cgaggcggtc aggggcaagc cggtcggcat gaagacccgg gaacgggcgc
    29761 aacagatcgg tttcgttctc cttgtaatgc tgatgatctt tgtcattatg attgatatcg
    29821 accggttgga tatcaagatt ataaacgatt tcacgaagat atttactaac gaaccatgaa
    29881 attgctcgcc ctggacactt cgggaaagac ggtctccgtt gccttcgtga gcgatgatgt
    29941 gacggtgggg gagatatttt ttgatgtgga acgtcatcat tccgaaatcc tccttcccgc
    30001 catcgatcat ctcctgaagg gggctggcgt ccggatcggt gatctggatc tcttcgcctg
    30061 cactgtcggt cccggttcct tcaccggcgt ccggatcggg gtaagcacgg tgaaaggcct
    30121 ggccctcgcc tacgaccggc ccatcgccgg cgtatccacc ctggaggccc tggcggtcaa
    30181 cgcggcgggc tttccgactc ttgtctgtcc cttgatcgat gcgaaacgtg gacaggtgta
    30241 tgccggtttt taccggagcg acaccgcagg ttctctaaca tgcctcagcc ccgactgcct
    30301 gcttgccgcc gaggaaatac cgaaccggat cgaaggggac gcggtcttcg tcggcagcgg
    30361 cgccggtgtt tataaagagc ttattagcgc ccggtccgaa ggccggagtc tgttcgcccc
    30421 cctacatttt caccggatca gcgccgctgc cgtagggatt gtcgcccgcc gcaaggctga
    30481 ggccggtgat ctccattccg ccgttcaact ggcgccggtg tatctccggc gggaaatgtg
    30541 aacggtcccg gaacaccggc gcaacgccga cggagtccgc atgggacggg atgtggagat
    30601 cgggtcccag gagtcccacc gtggtccccg ggaagcaatt tttaatgaaa cgcaccccgt
    30661 tatcggaaat agatgaccgc cgatgttgac agagaggttg aattccctta aaattgaaga
    30721 gtgatcaatt aaactgaaga gagaaagaca ccatgactca tgacagcccc atcgccccgg
    30781 agggcgtgcc cttcatcatc tcagcggtcc tcatcacttc ggtctgcctt tatttccagt
    30841 ggacttgggc ggcggtcgtt ttcggcctga ccaccctctg ggtggtatgg ttcttccgca
    30901 atccccggcg tacccctccc gctgatcccc gggccatcat ctccccggcg gacggcaagg
    30961 tcatccgcat cgaaacggtg gacggccatg agctgctgcc ggggccactg aacaaagtca
    31021 gcatcttcat gaacgtcttc agcgtccatg tcaatcgcat tcccttcggt ggcgtaatcg
    31081 atgagattcg ctattatccg ggaaaatttc ttacggcgaa tctggacaaa gcttcggagg
    31141 ataacgagcg caatgcccta cgcatcgata tcgggggagg ggagaagatc ctcgtcattc
    31201 agatcgccgg tctggtggcc cggagaatcg tctgctggac ggccaagggt atgaaggtga
    31261 acaaggggga gaggttcggc ctgattcgat tcggatcccg gctcgaggtc ttcatgcccc
    31321 cccgtgccca actccgtgtc caggtaggag aaaaggtgcg ggccggggaa acgcccatag
    31381 gagaattgcc atgaaagaac attcccgttt tcagaaagaa aagatgaaga aggggattta
    31441 tgtgctcccg aatctcttca ccacggccag tctctttgcc ggtttctact ccatgatcgc
    31501 ttctatgaag gggctgttcg aggttgccgc cgtcgccatc ctggttgccg ccgttctcga
    31561 cgccctggac ggaaggattg ccaggatgac caacacgacg agcaagttcg gcgccgaata
    31621 cgattccctg gcggatctcg tctccttcgg cgttgccccg gcccttctgg cctatacctg
    31681 gtcgttgcag gatttcggac gctggggttg ggtcgtatca tttctttttg ttacctgcgg
    31741 tgccttgcgt ctggcccgct tcaacatcca aatcggcatt attgacagcc gggtcttcaa
    31801 tggcctgccc atacccggcg ccgctgcggt agttgccacg ggcgtgctga tctactacaa
    31861 attgggaggg gttgggcatt tccatcatcc cctagtactg atctcggttt ttatcctggc
    31921 cctcttcatg gtgagcaaca taaagtatta cagtttcaag gacttgaatt tcttcgctaa
    31981 aaagccattc atgtcctttg tcgtcattgt ctgcatcctg gtagtggtaa tcgccgagcc
    32041 ccagatcatg attttcacct ttgtagcggg atacagcctc tcgggaccca tctggctgat
    32101 agtcaagcta taccggaaag gaacgtcggc ggcgacagag aaaaagagtg cggcggtgaa
    32161 aacggggccg caaagtagcc attaaacttc cgtaaggaga agacacagat atgaaagcct
    32221 ttaatgttgc agtagtcggc gccacaggcg ccgtgggtaa cgaaatggtc aagattctcg
    32281 aggaaagaaa ttttcccgtc gccaagctgc tcctgctcgc ttccgaacgc tccctgggca
    32341 aatccctgga atacaagggg cagagtcttc ccgtggaggt cctcaaggag gattctttca
    32401 cggatgtccg gatcggcctt ttctcagccg gaggcagcgt ttccgagaag tatgcccctc
    32461 tggccgcccg ggcgggatgc gtggtggtgg ataacaccag cgccttccgg atggcgcctg
    32521 atatccccct ggtggttccc gaggtgaatc ccgaagcgat cgcccagtac aagcagaggg
    32581 gcatcatcgc caaccccaat tgctccaccg tccagatggt ggtggccctg aaacctatcc
    32641 atgatgccgt ccgtattaag cggatcgtcg tttccactta tcaggccgtc tccgggaccg
    32701 gaaagaaggc catcgaggaa ttgagcaacc agacccgcgc cctgctgggg atgcgggaac
    32761 cggccgttaa ggtatatccc catcgcatcg ccttcaactg cctgccccag atcgatgttt
    32821 ttctggacaa cggctacacc aaggaagaga tgaagatggt aaacgaaacc aagaagatca
    32881 tgaaagacga ttcgatcgcc gtaaccgcga ctacggtcag ggtgcccgtt ttctatgccc
    32941 attcggaatc cataaacatc gagaccgaaa aaaagatcac cgccgccgag gtccgggaac
    33001 tgctggagaa ggccccgggc gtgaagatcg tcgatgaccc gagaaacctg gtgtatcctc
    33061 tggcgattga cgctgtcggt cgtgatgaaa cctttgtggg ccggatccgg gaggacgaat
    33121 cgatcaccaa cggcatcaac atgtgggtgg tttccgacaa cctccggaaa ggcgccgccc
    33181 tcaatgccgt ccagattgcg gaaatcctgg ttcgagacta cctgtgagag tgtcttcgag
    33241 catttggatg caactgcggg acgtttttta aacggagatt caagatatga gaataactgg
    33301 gggcgaaatg cggggaagac gaattccgcc ccccaagacc ggtggcatcc gtcccacgcc
    33361 ggacgccgtc cgggaatccc ttttcaatct ccttcctcct ctcaacggct gcaatttcct
    33421 cgatctttac gccg
//
LOCUS       DAOSMF010000014        30397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_1953_length_30397_cov_23.548645, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000014 DAOSMF010000000
VERSION     DAOSMF010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 30397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30397
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1953_length_30397_cov_23.548645"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /gene="dapF"
                     /locus_tag="PLT63_03210"
     CDS             <1..407
                     /gene="dapF"
                     /locus_tag="PLT63_03210"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOD97762.1"
                     /translation="INTGVPHVVHYVDDLDACDVFSLGREIRYHELYRPRGTNANFVK
                     ILASGTIAVRTYERGVEDETLACGTGSVAAAIIAAAKGLASSPVDVRVKSGEILRIHF
                     EKTGAGFRNVYLEGKTAVICSGRLWPEAYARG"
     gene            797..1966
                     /locus_tag="PLT63_03215"
     CDS             797..1966
                     /locus_tag="PLT63_03215"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HOD97763.1"
                     /translation="MEIAERLTKIPPYLFMELRKKIGAARAAGVDVISLAIGDPVEAT
                     PKSVIDELCRTAYDPENHRYPTDEEKGMLAFRQEAARWYDRRYGVALDPATEILGLIG
                     SKEGCHHFILARINPGDVVLMTDPGYPAYRASILMGGGEPWNVPILAKNDYLPDFADI
                     PTDIAKKARGMFLNYPNNPTGACATKKFLNDLVVFAKDYDIAICYDNPYSEILFAGQE
                     RISFLMAPGAKDVGVELNSLSKPFNMCGWRLGMACGNPDLIAAISKVKENTDSGIFNP
                     IQFAGIQALKNEEAFIEGMTALYGRRRELVLATLKKIGIDFAPPRGTFYLWVPVPKGM
                     TSLEFTNRLFDRTAIVVAAGTAYGKYGEGYIRISLTLPDDRLAEAMARIEKEFAP"
     gene            complement(2137..5637)
                     /locus_tag="PLT63_03220"
     CDS             complement(2137..5637)
                     /locus_tag="PLT63_03220"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOD97764.1"
                     /translation="MFFTVILWGIYGYLEMPKRKDPNIPGRVAVVLCPWPGAGAEKVE
                     QLLTRRIEEKIAENPKIKRIYTVARTGMAVIHLELLDNFKEAAKEFDDIKLRLDGIRD
                     LPENAGPIYFIKDYSDTAALMLTVASPRADEAELAIRTDAIRKAIAAARASAPPSGSG
                     PPAPRATLSVVFPFAIDPETLLPSFRILIRAIEKDGIMKKARIVKTAGYLGLDGETAL
                     DDAALLAYVDKYLKEHMQLAEFHPDIWEVGVIRKVEDVAAVLRKGAGDKYSFRQLDDM
                     TDLIVRTLKTVPDVAKVDRVGVQQEHIYLEYSQERLAAYGVNPMNIRDILGARNITGQ
                     GGVVEISGRNIAIDPSGEFKNEGEIGNVLLTAANSGAPMYLRDLLTITRSYENPVRFL
                     NFYTSKEADGKWRRSRAVTLAVQMREGHQIADFGRHVDEALAGIAGGLPADLVLARTS
                     DQPLQVKESIDLFMKSLYEAIVLVVLMAFIGFWEWRSAVLLSLSIPLTMAMTFGMMHL
                     LGIDMQQVSIASLIIALGLLVDDPVVAGDAIKRDLNAGHSPAVSAWLGPTKLATVIMY
                     ATITNIVAYLPFLLLKGDSGTFIYSLPVVLTCSLVASRIVSMTFIPFLAYYLLRPDKQ
                     APPTPEERRTRGFSGFYFRVGAAAIRHRWRVLLASLVFLVSGVFFMASLKMQYFPNDL
                     SYLSYVDIWLPEDASLGATFEAAGRTEEIIRQVAERYGKEHPDEDGKSREILASLTTF
                     IGGGGPRFWFSISPEMEQLNYAQIVILAKDKHDTSPLLGPLQTALVGAVPGARIDVRP
                     LETGAVVGIPVQIRISGNDIATLRRHADEIRDVLLKLPEASGVRDDWGTESLKVKYRI
                     DSDRANLARVSNADIAFSSATAVSGFPVAVLREGHRQIPVVMRLRMDERGQLGDLANL
                     YVSSSQEKFKVPLRQVAAIGYGMESEKIRRRDQFRTITVSCFPAEGYLASEVMDKARP
                     DIARIAKGLPSGYKLEIGGEEEEQEKGFKELTIVLATCVFSIFLALVFQFKNAVKPLL
                     VFAAIPFGMVGAYAGLYFMGLPFGFMAFLGIISLVGVIVSHIIVLFDFIEEMREKGEP
                     LEEALLDAGIVRLRPVMVTVGATVFGLVPLALHGGPLWEPLCYAQIGGLSLATFITLI
                     LVPVIYAISVLDLKIVKWEA"
     gene            5650..5928
                     /locus_tag="PLT63_03225"
     CDS             5650..5928
                     /locus_tag="PLT63_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97765.1"
                     /translation="MAVLDEKPGGIFGFFDMVFIRAVLHEPPVHKPGPAKQEKILQYG
                     GKIFMVTDRFNFSASIRRRWFYPNAHPEGSAGFQLRSRQNAAVIYLNA"
     gene            complement(5913..7106)
                     /locus_tag="PLT63_03230"
     CDS             complement(5913..7106)
                     /locus_tag="PLT63_03230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOD97766.1"
                     /translation="MSKENAQMHIPLPNESGPASRRDDRRVTVNGWMIVCVAVIAMQI
                     VWGCGDDQAKKKPLPKAVQVVTVQEEALAGGLRYSANIKPNRQVDLAFKNGGYVASLL
                     KVRGRDVQEGDRISRGQILATLRDRDYALKEAQTRAALTEAEAAWEQARQDYGRAENL
                     FASGSLTKPDFEAARARRDMLAAKTSAARAMHDEALLAVQDVSLRSPFSGVVLKKNIE
                     QGSLVGPGAAAFVVADTSVVKAEFGVPDVLLKHIRIGKTYTLISEAFPGEEFTGRIFR
                     IAAAADAGSRVFAVEVAIPNPRDQLKTGMVMSLVIDDAKLPKPAAVIPLNAVVRPRNN
                     PEAFAVFTIVPQGGRSIAKAKIVKLGEVVGNRMTVLEGLKAGEQIVGNGAALLQDGDE
                     IQAFK"
     gene            complement(7153..8508)
                     /locus_tag="PLT63_03235"
     CDS             complement(7153..8508)
                     /locus_tag="PLT63_03235"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOD97767.1"
                     /translation="MAWKRRILWLFTAGLVALGGQIAFVPAIAATPEILTLEDAVALA
                     LQNNRNVQNAAAEAEKTDAALAALRTRRYPNLNLRYTGAQQLNALDFEFKRGSLGNLS
                     SGEAIPTTDTIIPTPRQWENQGQAVMAQPLAQLYRLSLEIDQVGLAKTAAEEQYRQSR
                     TAVVNDVRKTYFGILESQSALRAVREAMGFYTELERLMTDHLRQQTILRADLLEVRSK
                     LARARYDATRTENALTAQKEQLNHLLGRDLATPFSVSAAVNPAPPEMDLAAAVARARE
                     RRPEIREAKLKARQAEYGIRIKRSEYIPDVDLIVSYTRLPEMEIMPKDYAYAGIRVSW
                     EPFDWGRRSQELSERKKTLAQANTAAREAESLVIMDVSQRWRALRESHQYLALTRLNA
                     EAARERLRVVMDRYRQQIALAKDLLQTQAQAAEADHQVHQALLACLAAHADFQKALGG
                     E"
     gene            complement(8696..9868)
                     /locus_tag="PLT63_03240"
     CDS             complement(8696..9868)
                     /locus_tag="PLT63_03240"
                     /inference="COORDINATES: protein motif:HMM:NF015400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HOD97768.1"
                     /translation="MNVLCLGAGGMGALAAETLAGFEEVGRMTVADLNPEAAQRVAAA
                     CRGKATARAVSVEDAAALTELMRGHDVVANCVGPFFRFAVPILSCAIAAGVNYFDICD
                     DPEPTLAMLKMGKEAEAAGITAVVGVGATPGITNMLAARIYPMFQKVEELHAAWNIEE
                     QDADKPEALTYSAAIVHWMQQCSGMILEHRNGRLQEVKPLEGVELYYPGRGTRTVWTV
                     GHPEPVAFSWSYPEIERSSCFMVMPSLTADYFRKLSKQIDAGRLTLEAAGHQLVDEAK
                     NVSFLDHILAVISGIFDHPRLPLFFVVAKGVIDGRKATVAATVKANPPGMARSTGIPL
                     AIGVHQFVRGMVKVKGVAAPEKALDAQAFFQELAPYCTFPKPAPDGDILDIVREWK"
     gene            complement(9897..10400)
                     /locus_tag="PLT63_03245"
     CDS             complement(9897..10400)
                     /locus_tag="PLT63_03245"
                     /inference="COORDINATES: protein motif:HMM:NF033684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter suffix domain-containing protein"
                     /protein_id="HOD97769.1"
                     /translation="MHPEKNWKYYLGLSLFGYSFVPILTVELLFFLPISHAEAASLAV
                     IYVGSGEVAFFAAIALLGRPFVDTLKTKLKSFFIRNKPARPPRPIGKTRHTIGVILFF
                     ASFLPYPFVVAALMFLHPTGRDIHYLVAALLAGDGIFITSLFVLGGEFWERLKRLFEW
                     PGKQQAA"
     gene            complement(10648..11091)
                     /locus_tag="PLT63_03250"
     CDS             complement(10648..11091)
                     /locus_tag="PLT63_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97770.1"
                     /translation="MMKRVVMYGAVILAAIFLLAGPAFCQDKPDATIKLSQGQVAVGI
                     GYSWGGGVLTYQGKEYPFKIRGLSVIDVGISRADAKGNVFKLKKLEDFNGTYTTLSAE
                     ATVAGGVGGVTMQNQNGVIIKLVAATQGVNFKLSLEGVKMTLTKK"
     gene            11337..12356
                     /locus_tag="PLT63_03255"
     CDS             11337..12356
                     /locus_tag="PLT63_03255"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOD97771.1"
                     /translation="MAQAKKKTTSNPAAHHPVTLLRQQLGEGEGQWVSGETLSQSLRM
                     SRTAVWKHVCALRTAGYRIDSSPRKGYLLREIPDLLLPQELQEGLQTALIGQGDIRFF
                     PRTDSTNRQAKALAAAGAPEGTVVIAEEQSAGRGRRGRAWFSPPRTGIYMSLILRPDI
                     PPQEAPRFALLAAAAVAAAVRETTSLEVMIKWPNDILAGGRKMGGILTEASMEMDRVE
                     YLVIGVGLNVNLNRREFPPELQSIATSIREETGRPAGRILLTKRLLESLESTYHEYRR
                     EGFAPILKRWQDFTEMLGREVAVEVRGRMLTGEVAAFDADGALVLRTPGGEELRLFFG
                     DVHFV"
     gene            12367..12903
                     /locus_tag="PLT63_03260"
     CDS             12367..12903
                     /locus_tag="PLT63_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HOD97772.1"
                     /translation="MKQNPPSLRGMAYASMFGALTAAGAFMMIPLPPVPITMQDFFVS
                     LAGALLGGYLGAMSQVVYILLGVIGLPVFAGGKAGFGVLLGPTGGYLIGFVVGAYVIG
                     RMTAGKDRPGFWRLIAAMTVGHLFLYALGIVQLMVVAKLTLAKALAVGLLPIIPGGIL
                     KIVMAAMICLKVREHIKL"
     gene            12914..13762
                     /locus_tag="PLT63_03265"
     CDS             12914..13762
                     /locus_tag="PLT63_03265"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOD97773.1"
                     /translation="MIEFKEVSYQYGERRPPVLERLSLRIPDGQYVVIIGPNGCGKTT
                     LSKHLNALLIPSRGEVSVDGLNTRDPSGHRELRRRVGMVFQNPDHQIVGMTVAEDIAF
                     GLENLAVPPGEIAIRVRDALARVEMAGCEQRAPFTLSGGEKRLVSLAGVLVMNPRWLV
                     LDEPTAYLDAAGRRLVLAMIASLHREGMGIVHITHDLGEAADAERILVLERGRVIMDG
                     PPGDVCAFLMSETQWEIEAPAMAALMAKLRRSGWDVPVNVISLEAAHREIHDYLVKIA
                     GRGASS"
     gene            13759..14553
                     /locus_tag="PLT63_03270"
     CDS             13759..14553
                     /locus_tag="PLT63_03270"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOD97774.1"
                     /translation="MSNMLSPVSAPGPSPLRNVDPRVKIVATVVLSILIFQAGPGQIV
                     LLSLFLALLIVFSGGGIRELGRILKPIAFFSVLLFFLHALFTPGREVFSLPILPAVTY
                     EGLVQGGTVAWQFAALVVTGGLLTLTTLPSELVAALDKILRPLQVFRVPTQDLTVMVA
                     MALRFAPTFLEEYQRMKTARLARGGGIPGERLSRKLQTSGRLAIALILSALRRAEDLA
                     AAMEARGYARGPRTSMRELCLNRGDYAAVAILASFTGLFFACRLFM"
     gene            14627..15355
                     /gene="fabG"
                     /locus_tag="PLT63_03275"
     CDS             14627..15355
                     /gene="fabG"
                     /locus_tag="PLT63_03275"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HOD97775.1"
                     /translation="MREENIVLVTGGGRGIGRAIVREFAAGGAYVVINYRQRQETAEA
                     TLGEIETEGGRGEICCFDVTDRALTERQIGGIISRLGRINVLVNNAGVTADNLFLMMP
                     ARDWDLVIDTALGGFFNVTKPVLRHMARKKSGAIVSIASVAGLVANRGQTNYSAAKAG
                     IIAASRSLATEVARFGIRVNVVAPGLVETDMISHAPVETIKEFIPMNRIGRPEEVARV
                     VRFLCSPDASYITGQVISVNGGMV"
     gene            15456..16403
                     /locus_tag="PLT63_03280"
     CDS             15456..16403
                     /locus_tag="PLT63_03280"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:NF012536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HOD97776.1"
                     /translation="MGEGISDYTKFCVDLRRHMWMAGVETELRRLIWQIAVTGKYISA
                     KIHESNRKLAGFKNMYGEEQLALDKSSDAILKNQLQFSGFVREYASEEQDQIIQIGKG
                     QEKYFVTADPLDGSSLVDTNLAIGTIIGIHNGTMYGKGRETMVAALYITYGPLITMVY
                     SAGKGTHEFVLNREGEYVLSQENIRLREKGDIYSLGGLRKDWTPEHLRFVADLEAAGY
                     KLRYSGGFVPDINQVLIKNGGVFTYPALRKSPQGKLRLLFELLPMAFLIEQAGGEATD
                     GKRRILDIPVEEIGQRSPIYIGSRYEVEKARKYLEEVEK"
     gene            16400..17794
                     /locus_tag="PLT63_03285"
     CDS             16400..17794
                     /locus_tag="PLT63_03285"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HOD97777.1"
                     /translation="MIYQQVGEVQKGMAGILKIAGEQVEINDGTGLRERAVDDLIHTA
                     VFSDDEAMRTAARWLIRRAGAAAGVLSASIQPLYEAMGRGEAKGFTVPAINIRALTYH
                     TAQAVLRAAMKLDVGPVIFEIARSEIDYTRQRPTEYIAAVTAAALKVGYRGPLFLQGD
                     HFQINAGKYAQNSEREVKAVKDLIWEAIEAGFYNIDVDTSTLVDLTQKTVKDQQRINY
                     TLAAELTAMIRDLEPAGITVSVGGEIGEVGGKNSTVEELAAFMAGYREELKKIDPAMK
                     GISKISIQTGTTHGGVPMPDGTVAKVKLDFATLEQLSREARATYGLAGAVQHGASTLP
                     PEAFDRFPAVGTAEVHLATGFQNIVYESPHLPADLKSRVYDHLRQTYRGEKKEGETEE
                     QFLYKTRKKGFGPFKKDFWDLPPATLAALGKELENQFSFLFTKLNMAGTKVLLDKYVP
                     PVDVPLQAPRDLVA"
     gene            complement(17957..19858)
                     /gene="metG"
                     /locus_tag="PLT63_03290"
     CDS             complement(17957..19858)
                     /gene="metG"
                     /locus_tag="PLT63_03290"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOD97778.1"
                     /translation="MDKTYYVTTPIYYVNASPHIGHAYTTIVADVLARYHKLSGYRTF
                     FMTGTDEHGDKIAEAAQKAGVDPKTYADGISAQFRDIWPELNVDHDYFIRTTDENHVR
                     VVRMILQQVYDAGDIYFGHYEGFYCVGCERFYMEKELVDGLCPDHQCEPEHRKESNYF
                     FRMSKYQEWLIKYIEEHPDFIRPERYRNEALAFLREPLEDLCISRPKSRLTWGITLPF
                     DENYVTYVWFDALINYITGVGYPDGENFRTFWPAVEHLIAKDILKPHGIYWPTMLKAA
                     GIEPYRHLNVHGYWNVDQSKMSKSLGNVIKPLDLKDKYGLDAFRYFLLRDMVFGLDSC
                     FSEENFIQRVNSDLANDLGNLISRVMAMAIKYFDGRVPAPAAPNDQDREFSTSARRTL
                     REVELCFQELTFHKALIAIWEFVNGANKYIVTSEPWNLAKEPGLKDRLATVIYNLLEA
                     LRCIAVFITPFMPGTAEKVLSHLGITDMAREDLSTLEEWGGLPVGNVLQKIAALFPRI
                     EYKKEEKMEAQTKAAPVFKPEISYEDFDKIDLRIARVLEAEPVPKSSKLLKLKIDLGE
                     ERVIVAGMGKDYKPEEIVGKMIVVVANLKPTKLMGVESQGMLLATDTAEGLALISADR
                     APVVGAKVR"
     gene            complement(19890..20684)
                     /locus_tag="PLT63_03295"
     CDS             complement(19890..20684)
                     /locus_tag="PLT63_03295"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HOD97779.1"
                     /translation="MSEEYLVGVKFKREGKVYYFHDAGIPLQKDDHVIVETDNGTVFG
                     TIVAERELASARGRTGAHKPILRKATDEDLQIQAENQVMEREARRFCVEKVTQRDLPM
                     KIVDVEYLFDRGKVVFYFTAENRVDFRELIKDLVQRFKTRIELRQIGARQETRMIKGM
                     GICGREVCCATMLHNLDRVSVKMAKEQSMSLNPEKISGLCGRLMCCLAFEYETYLGMK
                     KDMPKCGKFIQMDNTRAKVVRHSVLKGEITVAMENGEERTVKVADL"
     gene            complement(20779..21750)
                     /gene="holB"
                     /locus_tag="PLT63_03300"
     CDS             complement(20779..21750)
                     /gene="holB"
                     /locus_tag="PLT63_03300"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HOD97780.1"
                     /translation="MSGRGVYGHERQKEQLGAAIASGRLAHAYLFHGRDGIGKKEVAK
                     AFATAVLCGKGRPEACNACRSCKKVKSGNHPDLITVKPDGAFIKMQAVRELIGIMAFR
                     PMEGGRRLFLIDDADRLHPAAANALLKTLEEPAAANIIILITAKPYLLPRTIISRCQQ
                     LRFRPLRRETVARFLEEQAGLEATVTRALAASAEGSIARALQLQQEDYLADREGILTR
                     LASGHAGAPLPRLALFRYLGKSKKEIHDKLTLLQSCFRDALVWKELRDQGMLVHGDHL
                     DQVHGIARLTTDDLLFNLDAIAASLRALEANANKLLTLEAMTFTLRL"
     gene            complement(21773..22429)
                     /gene="tmk"
                     /locus_tag="PLT63_03305"
     CDS             complement(21773..22429)
                     /gene="tmk"
                     /locus_tag="PLT63_03305"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOD97781.1"
                     /translation="MGLFVTFEGIEGSGKTTQIELAGEYLGQRGIPCLRTEEPGGTSL
                     GRRLRELLLNRGPFAICPEAELLLFAAARAQHVREAILPALAAGKVVLCDRFADATAV
                     YQGYARGLDMETVNFLNAFSIQGRHPDLTLLFDLPVETGLTRARTRIELMTPGQKEDR
                     FEAEELSFHRKVREGYLLAAHREVERFRVIDAAGTIAELRETVCFHLDRMLMERGLNT
                     "
     gene            complement(22477..22656)
                     /locus_tag="PLT63_03310"
     CDS             complement(22477..22656)
                     /locus_tag="PLT63_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOD97782.1"
                     /translation="MAYIITDECIACGSCEDECPVEAISEGEDKYVIDPKVCTDCGAC
                     ADQCPVEAIIPGDDK"
     gene            complement(22698..22919)
                     /locus_tag="PLT63_03315"
     CDS             complement(22698..22919)
                     /locus_tag="PLT63_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOD97783.1"
                     /translation="MKAKVQRAIDRIRPNLQADGGDVQLLEVTDDGLVKVRLLGACHG
                     CPMAQMTLKNGIERYLKKEVPEVREVVSA"
     gene            complement(22943..23539)
                     /locus_tag="PLT63_03320"
     CDS             complement(22943..23539)
                     /locus_tag="PLT63_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97784.1"
                     /translation="MFTKSWWCFLILGWVIMIILACGGSLRYVERDPDLPDFHPRSIG
                     VLPVDAGGYDEARGIVEELVSAALTNKDWFAAVLPAATLKARLQGDQVLQQAVNDYLA
                     KLKTVNYSDPALSMKIGVALNMDAFLVVTVDSWSYAVENKEKVARAGLSMRLIDAQTG
                     KTAWRAGHDVVKEYTFLKPELKDLARNVVETMIGAMPH"
     gene            complement(23563..25311)
                     /gene="ispH"
                     /locus_tag="PLT63_03325"
     CDS             complement(23563..25311)
                     /gene="ispH"
                     /locus_tag="PLT63_03325"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOD97785.1"
                     /translation="MSVKLAETAGFCMGVKRAVDMVLELAQHKGQETIYTYGPLIHNP
                     QTVELLRKRGIVPIAAIEEIPAGTKAAGIIIRAHGISPQDRKLLREKNLKIIDATCPK
                     VGRVQAIIKKHAVRNYTILIIGDAGHPEVNGLLGYTFGKGLLLGAVEDVHRLPPLDKV
                     CVVAQTTQSVDEYRRIVTAIQARFPDAVIFDTICDSTERRQSEVKSLAEEMDAMIIVG
                     GRNSANTQRLADLAALPGKPTFHVETSEELPVEQLAAYEKIGISAGASTPNWIIDRVV
                     DTVTTRQADKSRQINTIFRFWVWSVRTDLYSALGAGCLAATAMLFQELTLRFLYIAVA
                     SLYVYAMHTLNRFINRKTGSIIGSFREESYRRHESKYLAAAILALVFALGAGLLSGRT
                     AFLFLLAMSTLGILYNAQLAPSGWPFRSIRDLPGSKNITVALAWAAITAVLPPVAEKL
                     PLTETTAVSFAFIFGIVFVRSVLSDLLGVQSDKLVGKETIPVLIGNDHTQQLLNMISL
                     ALVMMLAAAPTLVRTVSLSYFLIIPVIYIWICFRLYDKKPGLIGVVKEGVLETAYIIA
                     GLSSWVWYLIFGGKGL"
     gene            complement(25308..26045)
                     /locus_tag="PLT63_03330"
     CDS             complement(25308..26045)
                     /locus_tag="PLT63_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HOD97786.1"
                     /translation="MGKVRLDVLLVERQLVPSREKARAMIMTGAVLADEKRVDKAGTL
                     IDPRVELRIQREAEPYVSRGGRKLKGALDALAISVRDRVIIDVGASTGGFTDCLLQEG
                     AKKVYALDVGYGQLAWRLRQDPRVVPIERTNIRYYDGRDLLDIVDMATVDASFISLKL
                     VLPAVCPLIEGEGAILALIKPQFEVGKGMVGKNGVVSDPDLHRQVVGDLQTFVRTLGM
                     AVLGTCPSPLLGPAGNREFFLYAAKRS"
     gene            complement(26189..28099)
                     /gene="dxs"
                     /locus_tag="PLT63_03335"
     CDS             complement(26189..28099)
                     /gene="dxs"
                     /locus_tag="PLT63_03335"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOD97787.1"
                     /translation="MGNILAGINEPKDIRQLDIPSLSLLAQDIRELIITTVARTGGHL
                     ASSLGAVELTLALHYVFDTPRDKIVWDVGHQAYAHKIITGRREAFSTLRQQGGISGFP
                     KREESIYDVFNVGHSSTSISAATGMAEARRLKGENHRIVAVIGDGAMSAGMALEGLNW
                     AGGRKEDLIIILNDNEMSISPNVGALSSYLNRIMTGETVTRLRADLKTFLSSIPSIGG
                     HMVKFSRQFEEALKSFLLPGALFEDLGFIYVGPLQGHRLDRLIKNLHNIRQMKGPILV
                     HVLTKKGKGYQHAEEKPLRFHGTSSFCIETGRPTGGGNGSAPITYTGMFGETLLRLGR
                     ENSRIVAITAAMCNGTGLDEFSRAYPDRFFDVGIAEQHSVTFAAGLAVEGLIPVVAIY
                     STFLQRAYDQIIHDVCLQNLHVVFCLDRGGFVGDDGATHHGVFDLSYLRSIPNMMVMA
                     PKDENELQHMLKTAVDAGGPVAVRYPRGNGQGVPLDCEPHPLAIGRGEILCDGNELAV
                     IAIGATVYPALAAAEELRKEGIAIRVINARFVKPLDRELIRETAGKFKKLITVEENVR
                     MGGFGSAVMELLAVEEMSDVRIRILGIPDVFVEHATQQELRRQAGIDRNHILAAAREL
                     ISESGQRATVAC"
     gene            complement(28100..29011)
                     /locus_tag="PLT63_03340"
     CDS             complement(28100..29011)
                     /locus_tag="PLT63_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOD97788.1"
                     /translation="MAAETAYGDLDVYLARRKALVEEALDRFLSPETGVPERIMDAAR
                     YSLFAGGKRLRPILCLAAAEVVSGAWSDALPCACALEMIHTYSLIHDDLPAMDDDDYR
                     RGRPTNHIVFGEGLAILAGDALLTEAFHLLARRDLMPGVVPEDRLEVIRLVARAAGMA
                     GMVGGQVMDLQGEGQDLELPTLTDMHRRKTGALLVVSVVAGAVIAGASDGQKEILAGY
                     GEKIGLAFQIADDILNVTGDRVMMGKSTGSDAVRGKQTFPALLGLEASRTKLADLVDG
                     AAADARRLGERCEPLVQIAKYIMERNT"
     gene            complement(29013..29240)
                     /locus_tag="PLT63_03345"
     CDS             complement(29013..29240)
                     /locus_tag="PLT63_03345"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOD97789.1"
                     /translation="MAKEKFEEALKKLEELVKTMEAGDLTLDESLKAFEEGIRLIRFC
                     TRKLDEAERKIEILLRDDDDRLAAVPFPTEG"
     gene            complement(29410..>30397)
                     /gene="xseA"
                     /locus_tag="PLT63_03350"
     CDS             complement(29410..>30397)
                     /gene="xseA"
                     /locus_tag="PLT63_03350"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOD97790.1"
                     /translation="PFLPRGIGVITSPTGAVIRDIMQITGRRFPSVPILIAPVRVQGP
                     EAAPEIVRALALLNGESGEPGAYPDLEVIIIARGGGSLEDLYPFNTEEVARAVFRSRL
                     PVVSAVGHETDYTITDFVADLRAPTPSAAAEMVVPDREHLRRQVGDAYGSLLQARQRW
                     VDRWRERIEAAAGQLKSPDRILADYRLLLDDHRERLFLALNRHIISRRHCLQYLRTRL
                     EHVCPTGTIDRIRVVLAHRREQFATGIGAFFRQQRAVMDRQGVSLQALNPLQVLRRGY
                     SITRRFPDGLIVKRAADLATGDRLSVRLAAGEIRARVEVVAPEEMKESGSLF"
BASE COUNT         6155 a         9226 c         8804 g         6212 t
ORIGIN      
        1 gcatcaacac cggggttccc catgtggtcc attatgtgga cgacctggat gcctgtgacg
       61 tcttttccct cggccgggaa atccggtatc atgagctcta ccggccccgg gggacgaacg
      121 ccaatttcgt aaagatactc gcttcgggaa cgatcgccgt ccgcacttac gagcggggcg
      181 tggaggatga aaccctggcc tgcggcaccg gctccgtggc ggccgcgatc atcgccgccg
      241 ccaagggact ggcgtcgtct cccgtggatg tccgggtaaa aagcggcgag atccttcgga
      301 tccacttcga aaagaccggc gccggattca ggaatgtcta tctggagggg aaaacggcgg
      361 taatctgttc cggtcgcctc tggccggagg cgtatgcccg aggttgattt caccggaaca
      421 aggaaatccg ccgaactcac cggggccgcc ccggggacat ccgacgacgc caaacccggg
      481 gcggaaagac cgcgcccggg cccgccgtca ccatgccggc ttccgcggca attcgatcga
      541 gtgtcgtttc aacaatgaaa agactcatgc aaataaatat ttactaatag ttacaatatg
      601 ttaccgctcc gaaagggtgc tcggcaaggt tgtcgcgaca cttgcctgac ggtccggcgg
      661 tgggaccgca aggacgggat tgagtgatcg gccagtattt gaggttctca ggggaggttt
      721 ctattgacga ttcccgccct tttcctatat agacaccccc agaaaaatcc gattgtgacc
      781 gaggaggata aacaccgtgg agattgccga acgtttgacc aagatccccc cttacctgtt
      841 catggagctg cgaaagaaga tcggcgccgc ccgggccgcc ggcgtggacg tcatcagcct
      901 ggccatcggc gaccccgtgg aggccacccc gaagtccgtc atcgatgagc tctgccggac
      961 ggcctacgac ccggagaatc accgctaccc cacggacgag gaaaagggga tgctggcctt
     1021 ccgccaggag gcggcccggt ggtacgatcg ccgttacggc gtcgccctcg atcccgccac
     1081 ggagatcctg gggctcatcg gttccaagga ggggtgtcat catttcatcc tggcccggat
     1141 aaatcccggc gacgtggtcc tcatgaccga tcccggctac cccgcctacc gggcaagcat
     1201 cctcatgggc ggcggcgagc cctggaacgt cccgattctg gccaaaaatg actatctccc
     1261 cgactttgcc gacattccca cggatatcgc caaaaaggcc cgggggatgt ttctcaacta
     1321 ccccaacaat cctaccgggg cctgcgccac aaagaaattt ctgaacgatc tcgtggtgtt
     1381 tgccaaagat tacgatatcg ccatctgcta tgacaacccc tacagcgaga tcctttttgc
     1441 cggccaggag cggatcagct ttctcatggc cccgggggcg aaggacgtgg gggtggagtt
     1501 gaactccctc tccaagcctt tcaacatgtg cggctggcgc ctgggcatgg cctgcggcaa
     1561 tccggacctc atcgccgcca tcagcaaggt gaaggaaaat accgattcgg gcatcttcaa
     1621 ccccatccag ttcgccggga ttcaggccct gaaaaacgag gaggccttca tcgagggcat
     1681 gacggcgctc tacggtcggc gccgggagct ggtcctggcc accctgaaga agatcggcat
     1741 cgactttgcg ccgccccggg gcaccttcta cctgtgggtg ccggtcccga aggggatgac
     1801 ctcgctggaa ttcaccaacc gcctcttcga ccggacggcc atcgtcgtgg ccgccggcac
     1861 cgcctacggc aaatacggcg aaggctacat ccggatatcc ctcacgctcc ctgacgaccg
     1921 ccttgccgaa gccatggctc gcatcgagaa agagttcgca ccctgacacc cctgaggccg
     1981 ggcgccggcg gcggactgtc gtcatggagc gcattggcaa agggttgacg gcgcctcaag
     2041 gcgccggaac cttcacttct cgttgatact tattaatgtc tttaccctga cgacaatagc
     2101 caatgtcccg agacgcgcca aaagaaaaac gggggatcat gcctcccact tgacgatctt
     2161 caggtcgagg acggaaatgg cgtagatgac cggcacgagg atgagggtga taaaggtcgc
     2221 caggctcaat cccccgatct gggcatagca gaggggctcc cacagcgggc cgccgtgaag
     2281 ggccaggggc accagaccga agaccgtggc gccgacggtg accattacgg gccgcagccg
     2341 cacgatcccg gcatccagca gggcctcctc aaggggctcc cccttctccc gcatctcctc
     2401 gataaaatcg aacaggacga taatgtggct gacgattacg cccacgaggc tgatgatccc
     2461 cagaaaggcc atgaaaccga agggcaggcc catgaaatag aggccggcat aggccccaac
     2521 cattccgaag ggaatggcgg caaagacgag cagcggcttg acggcgttct tgaactggaa
     2581 gaccagggcc aggaagatgg agaagacgca ggtggccagc acgatggtca actccttgaa
     2641 gcccttttcc tgctcctcct cttcgccgcc gatctccagt ttgtatcccg acggcagccc
     2701 tttggcgatc cgggcgatat ccggccgcgc cttatccatc acctccgaag cgagataacc
     2761 ttccgcgggg aagcaggaaa cggttatggt ccggaactga tcccgccttc tgatcttttc
     2821 cgattccatg ccatacccta tggccgccac ctgccgcagg ggcaccttga acttttcctg
     2881 ggatgaggag acatagagat tggccaggtc accaagctgt cccctttcat ccatgcggag
     2941 tctcataacg acggggatct gacggtgtcc ttcccggagg acagcgacgg ggaaaccgct
     3001 gaccgccgta gcggaggaaa aggcgatatc ggcgttggat acccgggcca gattggcccg
     3061 gtcggagtcg atgcggtact tgaccttgag actctccgta ccccagtcat cccggactcc
     3121 cgaggcctcg ggaagcttca gtaagacatc ccggatttcg tcggcatgcc tgcgcaaggt
     3181 ggcgatatcg ttcccggaaa tcctgatctg gacaggtatt cccaccacgg cccccgtttc
     3241 caggggccgc acgtcgatcc gcgcccccgg aacagcacca accagcgccg tctgcagcgg
     3301 tcccaacagc ggggatgtgt catgtttgtc ctttgccagg atgacgatct gggcgtaatt
     3361 gagctgttcc atctcaggac tgatggaaaa ccagaaccgg gggcctcctc cacctataaa
     3421 ggtagtcagg gaagccagga tctcccgtga ttttccgtct tcatcgggat gttccttgcc
     3481 gtaccgctcc gccacctggc ggatgatctc ctccgtacgc cccgccgctt caaaggttgc
     3541 gccgagggag gcgtcttccg gcagccagat atccacatag gacaagtaag acagatcatt
     3601 gggaaagtac tgcatcttca ggctggccat gaagaagaca ccggagacga gaaagaccag
     3661 ggaggccagg aggacccgcc agcggtgccg gatcgccgcg gcgccgacgc ggaaatagaa
     3721 tcccgaaaat ccccgggtgc gccgttcttc cggagtcggg ggcgcctgtt tgtccggacg
     3781 gaggagatag taggccagaa acgggatgaa ggtcatggaa acgatccggg aggccacgag
     3841 cgaacaggtg agaaccacgg gaaggctgta gatgaaggtt cccgaatccc ccttgaggag
     3901 gaggaagggc aggtaggcca cgatattggt gatcgtcgca tacatgatga ccgtggcgag
     3961 cttggtgggc cccagccagg cggagaccgc cggggaatgg ccggcgttga ggtcccgctt
     4021 gatggcgtcc cccgccacca ccgggtcatc tacaaggagt cccagggcga tgatgaggga
     4081 ggcgatggat acctgctgca tgtcgatgcc cagcagatgc atcatcccga aggtcatggc
     4141 catggtgagg ggaatggaca gggagaggag gaccgcggag cgccattccc aaaaaccgat
     4201 gaaggccatg aggaccacca ggacgatggc ctcatagaga ctcttcatga agaggtcgat
     4261 gctttccttc acctggaggg gctgatcgga ggtccgggcc aaaaccagat ccgccggcag
     4321 ccccccggcg atccccgcca gggcctcgtc cacatgacgg ccaaaatcag cgatctgatg
     4381 cccttcccgc atctgcacgg caagggtgac ggcccggctc cgccgccatt tgccgtccgc
     4441 ctccttgctg gtatagaagt tgaggaagcg gacgggattc tcatagctcc gggtaatagt
     4501 cagcagatcc cggagataca tgggggcccc ggaattggcg gcggtgagga ggacgttgcc
     4561 gatctctcct tcgttcttga actctccgga gggatcgatg gcgatgttcc ggccggagat
     4621 ctccaccacg ccgccctgac cggtgatgtt tctcgctccc agaatgtccc ggatattcat
     4681 cggatttacc ccgtaagccg caagtcgctc ctgagagtat tccagataaa tatgttcctg
     4741 ctgcaccccc acccggtcca ccttggccac atcagggacc gtcttcaggg tccggacaat
     4801 gagatccgtc atgtcgtcga gctggcggaa gctgtacttg tctccggcgc ccttccggag
     4861 caccgccgcc acgtcttcga ccttccggat tacgcccact tcccaaatgt cggggtgaaa
     4921 ctccgccaac tgcatatgtt ccttaagata tttgtctaca taggcgagca gcgccgcgtc
     4981 gtccaacgcc gtttcgccat ccaggccgag gtatcccgcc gtcttgacga tccttgcctt
     5041 cttcatgatc ccgtctttct cgatggcccg gatgaggatc cggaaagagg ggaggagtgt
     5101 ttcgggatca atggcgaagg ggaaaactac ggacagcgtc gcccggggcg ccggcggtcc
     5161 gcttccggaa ggcggagcgg aggcccgggc ggcggcaatg gccttgcgga tcgcgtccgt
     5221 ccggatagcc agctcggctt cgtcggccct ggggctggcc acggtcagca tcagggcggc
     5281 ggtatcggaa taatccttga tgaaatatat cggaccggcg ttctccggaa gatcccggat
     5341 accgtccaga cgcagcttga tatcgtcgaa ttccttggcc gcctccttga aattgtccag
     5401 caattccaga tggatcaccg ccatccccgt ccgggcgacg gtgtagatcc gtttgatctt
     5461 gggattttcc gcgatctttt cttcgatccg ccgggtcagg agctgttcga ccttttccgc
     5521 ccccgccccc ggccaggggc agaggaccac ggccacgcgg ccgggaatgt tggggtcctt
     5581 gcgcttgggc atctccagat agccatagat gccccagagg ataacggtaa aaaacaatat
     5641 ccaggagata tggcggtgct cgacgaaaaa cctggcggta tttttggttt tttcgatatg
     5701 gtcttcatcc gagcggtgct tcatgaaccc cccgtgcaca agcccggccc agccaaacag
     5761 gaaaaaattc tacagtatgg cggaaaaatt ttcatggtaa cagaccgttt taatttttcg
     5821 gcatcgatac gaagaagatg gttttacccc aatgctcatc ctgaaggcag cgcgggcttt
     5881 cagctccgct cacggcaaaa cgccgccgtc atctatttga acgcctgaat ctcgtccccg
     5941 tcctggagaa gagcggcgcc gttgcccact atttgctccc ccgccttcaa accttccagg
     6001 accgtcatcc ggtttcccac cacctcgccc agcttcacga tcttcgcctt ggcaatggat
     6061 ctgcctccct ggggaacgat cgtgaagacc gcaaaggctt ccgggttgtt ccgggggcgg
     6121 acgacggcat tgaggggaat gaccgcggcc ggtttgggga gtttggcgtc atcgatcacc
     6181 aaggacatga ccatgcccgt tttgagctgg tcccggggat tggggatggc gacctccacg
     6241 gcaaagaccc ggctgccggc atcggcggcg gcggcgatgc ggaatatccg gccggtgaac
     6301 tcctcaccgg ggaaggcctc gctgatcagc gtatatgtct tgccgatccg gatatgcttg
     6361 aggagcacgt cgggcacccc gaactccgcc ttcaccacgg aggtgtcggc caccacgaag
     6421 gcagcggcgc cgggcccgac cagactcccc tgttcgatat tctttttcag gaccactccg
     6481 gagaaggggg aacggaggga gacatcctgt acggccagaa gggcctcgtc atgcatcgcc
     6541 cgggctgccg acgtttttgc cgccagcatg tcccgacggg cccgggcggc ctcgaaatct
     6601 ggcttcgtaa ggctgccgga ggcaaagaga ttctcggccc gtccgtaatc ctggcgcgcc
     6661 tgctcccagg cggcttcggc ctcggtaagg gccgcccggg tctgggcctc tttgagggcg
     6721 tagtcccggt cccggagggt tgccaggatc tggccccggg agatccggtc cccttcctga
     6781 acatcccggc cccgcacctt aaggagagag gctacatagc ccccgttctt gaaagcgaga
     6841 tccacctggc gattcggttt gatgttggcg gaatagcgga gtccccccgc cagggcctct
     6901 tcctggacgg tgacgacctg cacggccttg ggaagaggct tttttttcgc ctggtcatcg
     6961 ccgcaacccc agacaatctg catcgctatc acagcgacac agacaatcat ccacccgttg
     7021 accgtcaccc tccggtcatc ccgccgggag gccggaccgc tttcgttcgg aagcggtata
     7081 tgcatctggg cattttcctt gctcatctat cctttcctgt ctccatcgct tttccgccgc
     7141 cggagaaagc cctcattccc cccccagggc cttctggaaa tcggcatggg cggccaggca
     7201 ggccaaaagg gcctgatgga cctgatgatc cgcttcggcc gcctgggcct gggtctgaag
     7261 gagatccttg gcaagggcga tctgctgccg atagcggtcc ataaccaccc ggagccgctc
     7321 ccgggccgcc tctgcattca gcctcgtcag cgcgagatat tgatggctct cccgcaacgc
     7381 ccgccaccgc tgggaaacat ccattatcac cagcgattcc gcctcccggg cggcggtatt
     7441 ggcctgggcc agggtcttct tgcgttccga aagctcctga gacctccgtc cccaatcgaa
     7501 gggctcccaa gagacccgga tgccggcata ggcgtaatcc ttgggcatga tctccatctc
     7561 cggcaaacgg gtgtaagaaa caatcagatc gacatcgggg atatattccg agcgcttgat
     7621 cctgatcccg tactccgcct ggcgggcctt gagtttcgcc tccctgatct ccggccgtcg
     7681 ctccctggcc ctcgccaccg ccgccgccag gtccatctcc ggcggcgccg gattcacggc
     7741 cgcgcttacg gaaaagggcg tggcgaggtc cctccccagg aggtggttga gttgttcctt
     7801 ctgcgccgtc agggcatttt ctgtacgggt ggcatcgtaa cgggcccggg ccagcttcga
     7861 gcggacctcc agcagatcgg cccggaggat ggtctgctgc cggagatggt cggtcatcaa
     7921 ccgttccagt tccgtataga agcccatggc ctctcgaacg gcccggaggg cgctctggct
     7981 ttccaggatg ccgaaatagg tcttccggac atcgttgacc accgccgtcc ggctttggcg
     8041 atactgttct tcggcggcgg tttttgccag cccgacctga tcgatctcca gggacaggcg
     8101 gtacaactgg gcaagaggtt gggccatcac tgcctgtccc tggttttccc actgcctggg
     8161 agtggggatg atcgtatccg tggtgggaat ggcctccccg gaagacagat tgcccagtga
     8221 tcctcgcttg aactcgaaat ccagggcatt caactgctgg gccccggtgt agcgcagatt
     8281 aagatttgga tagcgacggg tgcggagggc ggccagggcg gcgtcggttt tctccgcctc
     8341 cgccgcggca ttctggacat tccggttgtt ttgcagggcc agggccacgg cgtcttccag
     8401 agtcagtatc tcgggggttg cggcaatggc cgggacaaag gcaatctgcc cccccagggc
     8461 taccagtccc gctgtgaaca accacaagat ccgtcttttc caggccattt gccgttttcc
     8521 catccccggt ggaacgccgg ggtttaggcc gacgtcgcgt caattatgac ttgatccagc
     8581 ccgcagataa tccgtctccc cgcccggcta acaagggcgg tttttggccg ggaagatcat
     8641 gatacgccga ttttcatcac tcgccggcat caagatctat cggcccaacc ctggtttact
     8701 tccactcccg gacaatgtcc aggatgtcgc catccggcgc cggtttcggg aaggtgcaat
     8761 aaggggccag ttcctgaaaa aaggcctggg catccagggc cttttccggc gccgccactc
     8821 ctttgacctt gaccatcccg cggacaaact ggtgtacacc gatggccaga gggatgccag
     8881 ttgacctggc catgccgggg gggtttgcct ttacggtcgc ggctacagtg gccttacggc
     8941 catcgatgac gcctttggcc acgacgaaga acaggggcag gcggggatga tcgaagatac
     9001 ccgagatcac cgcgaggata tgatccagaa aggagacgtt tttggcttcg tccaccagtt
     9061 gatgccccgc cgcttccagg gtcaggcggc cggcgtcgat ctgtttgctc aatttccgga
     9121 aataatcggc ggtcagggaa ggcatgacca tgaaacagga ggaccgttct atctccggat
     9181 atgaccagga gaaggccacc ggttccggat gacccaccgt ccacaccgtc cgcgtgcccc
     9241 gtccgggata gtacagctcc accccttcca gcggtttcac ctcctgcagt cggccattcc
     9301 ggtgttccag gatcatcccc gagcactgct gcatccagtg tacgatagcg gcggaatagg
     9361 tcagcgcctc gggcttgtcg gcatcctgtt cctcgatgtt ccaggcggcg tgcagttcct
     9421 ccaccttttg aaacattgga tatatcctcg ccgcgagcat gttcgttatc cccggggtgg
     9481 ccccgacgcc gaccaccgcc gtgatccccg ccgcttccgc ctcctttccc atcttcagca
     9541 tcgccagcgt cggctccgga tcgtcacaga tgtcgaaata gttcactccc gccgcgatgg
     9601 cgcaagaaag gatgggaacg gcaaaacgga agaagggccc cacgcagttg gccacaacat
     9661 catggccccg catcaattcg gtcagggccg cggcgtcttc cacgctgacg gcacgggccg
     9721 tcgccttgcc ccgacaggcg gccgccaccc gctgcgccgc ctccggattg agatcggcaa
     9781 cggtcatcct gccgacttct tcaaaacccg ccagcgtctc cgccgccagc gcccccatgc
     9841 cgcctgcccc cagacacaag acgttcataa tccccctcct tgattgttcc tttaaattat
     9901 gccgcctgct gcttccccgg ccattcgaaa agccttttca accgctccca gaattccccc
     9961 cccaggacaa acagactcgt gataaatatc ccgtctccgg ccaacagggc ggcgacgaga
    10021 taatgaatat cccgccccgt ggggtgcaaa aacatgaggg ccgccaccac aaacggatag
    10081 gggagaaaac tcgcaaagaa aaggattacc ccgatggtat gtcgggtttt gccgatggga
    10141 cggggaggtc gggcgggttt attgcggatg aaaaaggact tcaacttggt cttgagggtg
    10201 tccacaaacg gccggcccaa gagggctatg gcggcaaaaa aggccacctc tccagaaccg
    10261 acatagatca ccgccagcga agccgcctcg gcatgggaaa tgggaaggaa gaagagcagt
    10321 tcgaccgtca agatgggaac aaacgaatat ccgaaaaggg acaaccccag gtaatatttc
    10381 cagttttttt caggatgcat tacatgagca cacgatcata tcggttgttc ggtttagctt
    10441 tctgaaaagc cggccatcat tggaaagctg attgtggcca gaaaatatac aaaccccttt
    10501 tgcttgtcaa tgagaaacat gtaaatgtca actttaccgt tcagggcggc gctgaggatt
    10561 gtatatcaat ggccatgaaa aaaaggccgg tggaaagcat tgctggcgct cttcaccggc
    10621 ctgaaaggtc gttttctcta tatggatcta tttcttggtc agggtcatct tgacaccttc
    10681 cagggagagt ttgaaattga cgccctgggt cgccgccact aacttgataa taaccccatt
    10741 ctgattctgc atggtaacgc cacccacgcc tccggccacc gtggcctcgg cggaaagggt
    10801 ggtgtaggtc ccgttaaaat cctccagctt cttgagtttg aagacatttc ctttggcatc
    10861 cgcccggctg atgcccacat cgataacgga gagccccctg attttaaagg gatattcttt
    10921 cccctgatag gtcaggacgc cgccgcccca gctatagccg atacccactg ctacctgccc
    10981 ctgagagagc ttgatggtgg cgtcgggctt gtcctggcaa aaagccggac ccgcaagcaa
    11041 aaaaattgcc gccagaataa cagcaccata cataacaacc cgtttcatca tttatcctcc
    11101 tttttcactt tttttgggaa ttgccttccc atttgaattt tcaggtctct cgtgtatttt
    11161 ctaattacac catttttcca ttttttcata caaaattatg tcgttgttcc cggcaagatt
    11221 atatcgccga tcatcacctt ccgggcatta acaaccattt accatttatt ggttgacaat
    11281 caaccgcata tccattatga accttgcggt ttcagggaag actctatttt aaagttatgg
    11341 cacaagcaaa gaagaaaact acctccaatc ccgcggccca ccatcccgtc acgttgctcc
    11401 ggcagcaatt aggggagggg gagggccagt gggtgtccgg tgaaaccctg agccaatcct
    11461 tgcggatgag ccgtaccgca gtgtggaagc atgtctgtgc cctgagaacg gccggatatc
    11521 gcatcgactc ctcgccgcga aagggctatc ttctccggga gatacccgac ctgcttctgc
    11581 cccaagaact tcaggaggga ttgcaaaccg ccctgattgg ccagggggat atccggtttt
    11641 tcccccggac ggattcgacg aaccggcagg ctaaagccct ggccgccgcc ggggcgccgg
    11701 aaggaacggt ggtgatagcg gaagaacaga gcgctggccg gggccggcgg gggagggcct
    11761 ggttttcgcc tccccggacg ggcatctata tgtccctgat cctccggccg gacataccgc
    11821 ctcaggaagc gccccggttt gccctcctgg ccgcagcggc ggtggcggcg gcggtccggg
    11881 aaacgaccag cctggaggtt atgataaaat ggccaaacga tatcctggcc ggcgggcgga
    11941 agatgggagg catcctcacc gaggccagca tggaaatgga ccgggtggag tacctggtca
    12001 tcggcgtggg attgaacgtg aatttgaacc ggcgggagtt tcccccagaa cttcagtcca
    12061 tcgccacaag catccgggag gagaccggcc gaccggctgg caggatactc ctgacaaaaa
    12121 ggcttctgga aagcctggaa tcgacatacc acgagtaccg gcgggaaggt ttcgcgccaa
    12181 ttttgaagcg ctggcaggat tttaccgaga tgttgggccg tgaggtggcg gtggaggtca
    12241 ggggacggat gctcaccggc gaggttgcgg ctttcgacgc tgacggcgca ctggtcctcc
    12301 ggaccccggg cggggaggag ttgcggctct ttttcggaga cgtgcatttt gtttaggggg
    12361 ccggcgatga aacagaatcc accgtcgctc aggggcatgg cctatgcatc catgttcggc
    12421 gccctgaccg ccgccggggc cttcatgatg atccccctgc ccccggtgcc catcaccatg
    12481 caggactttt tcgtttccct ggccggcgcc ctcctggggg gatacctcgg ggccatgagc
    12541 caggtggtgt atatcctcct cggggtgatc ggcctgcccg ttttcgccgg cggcaaggcc
    12601 gggttcgggg tcttgctggg gcccaccggg ggttatctca tcggctttgt ggtcggggcc
    12661 tatgtcatcg gccggatgac cgcagggaag gatcgtcccg gtttctggcg gctgatcgca
    12721 gccatgacgg tgggccatct gttcctctac gccctgggaa tcgttcagtt gatggtcgtg
    12781 gcgaaattga ccctggccaa agccctggcc gtcggcctgc tccccatcat tcccggaggt
    12841 atactgaaaa tcgtcatggc ggccatgatc tgtctcaaag tgcgggagca catcaaactg
    12901 taagttatcg gcaatgatcg aattcaaaga agtttcctac caatatggtg aaaggcggcc
    12961 gcccgttctg gagcggttgt cgttgcggat tcccgacggt cagtatgtgg tgatcatcgg
    13021 ccccaacggc tgcgggaaga ccaccctgag caagcacctg aacgcccttt tgatcccctc
    13081 ccggggtgag gtcagcgtcg atggcctgaa cacccgggac ccctcggggc atcgggaact
    13141 ccgccgccgg gtgggaatgg tttttcaaaa tcccgatcat cagatcgtgg gcatgacggt
    13201 ggcggaggat atcgccttcg ggctggagaa tctggccgta ccccccggag aaatcgccat
    13261 ccgtgtccgg gacgccctgg cccgggtcga gatggccggc tgcgagcagc gggcaccctt
    13321 caccctttcc ggcggggaaa agaggctggt ctccctggcg ggggtcctgg tgatgaatcc
    13381 ccgttggctg gtcctggacg agccaacggc ctacctcgat gcggcggggc ggcgcctggt
    13441 gctcgccatg atcgcctccc tccaccggga gggaatggga atcgtccata tcacccacga
    13501 cctcggcgag gccgccgatg ccgagcggat cctggtcctg gaaaggggcc gggtaatcat
    13561 ggacggcccc cctggtgatg tttgcgcctt cctgatgtcg gaaacacaat gggaaatcga
    13621 ggccccggcc atggccgccc tgatggcaaa gctccggcgg tcgggatggg acgtgccggt
    13681 gaatgtcatc tccctggagg cggcgcaccg ggagattcat gattatctgg taaaaattgc
    13741 cggaagggga gcgtcctcat gagcaatatg ctctccccgg tctccgcgcc cggaccgtcg
    13801 ccactccgca acgtcgaccc ccgcgtaaag atcgtcgcta cggtggttct gagcatcctt
    13861 atctttcagg ccggtcccgg tcaaatcgtt ctcctcagcc tctttctggc cctcctgatc
    13921 gttttttccg gggggggcat ccgcgaactg ggaaggatcc tcaaacccat cgcctttttt
    13981 tccgtcctgc tctttttcct ccacgccttg tttaccccgg gccgggaggt cttcagcttg
    14041 cccatattac cggcggtgac ctacgaaggt ctggtgcagg gggggaccgt ggcctggcag
    14101 tttgccgccc tggtcgttac cggcggtctg ctcaccctta ccaccctccc ctccgaactg
    14161 gtcgcagccc tagacaagat cctccgcccc ctgcaggtct tccgtgtacc tacccaggat
    14221 ctgaccgtta tggtggccat ggccctgcgc ttcgccccca cctttctgga agagtaccag
    14281 cgtatgaaaa cggcccggct ggcccgtggc ggagggatcc ccggagagcg tctgtccaga
    14341 aaattgcaaa cttccgggcg cctggcgata gcactgatcc tcagtgccct ccgccgggcc
    14401 gaagacttgg ccgcggccat ggaggcccgg ggttatgccc ggggtccccg gacctccatg
    14461 cgggaactgt gcctcaacag gggggattac gccgccgtcg ccatccttgc ttctttcacc
    14521 ggcctgttct tcgcttgccg gcttttcatg taaatcccgt ccggagcgag gtttttcttg
    14581 acttgtccgg aaaatatgct tctatgcatt gagcggaggt aaagacgtgc gcgaagaaaa
    14641 tatcgtcctc gtaaccgggg ggggccgggg catcggccgg gctatcgtcc gggagtttgc
    14701 cgccggtggc gcctatgtgg tcataaatta ccgccaaagg caggaaactg cggaagctac
    14761 cctgggcgag atcgagacag agggaggccg gggggaaatc tgctgcttcg atgtcacgga
    14821 ccgggcctta acggagcggc agataggtgg aattatctcc cgcctcggcc gcataaatgt
    14881 cttggtcaac aatgccggcg tcaccgccga caaccttttt ctcatgatgc ccgcccggga
    14941 ctgggacctg gttatcgaca ccgccctcgg gggatttttc aacgtaacca agcccgttct
    15001 ccgccatatg gcccggaaaa aatccggggc tatcgtctcc attgcctccg tggccggtct
    15061 tgtcgccaat cgcggtcaga ccaactattc ggcggccaag gccggcatca ttgccgccag
    15121 ccgttccctg gccacggaag tggcccggtt cggcatccgg gtaaacgtcg tggctcccgg
    15181 actggtggaa acggacatga tcagccatgc cccggtggaa acgatcaagg agttcattcc
    15241 catgaaccgc atcggccggc cggaggaagt ggcccgggta gtccggttcc tctgctcccc
    15301 tgatgcctcc tatatcaccg gccaggtgat ctccgtcaac ggcgggatgg tgtaagccgt
    15361 tcattttcat cttgtcaaaa acctcattaa ttgctaatta cgggcgaaat catgaacatt
    15421 aatcagcgga agctgatttg cgaggttgat agcttatggg cgagggaatt tccgactata
    15481 caaaattttg cgtagatctt cgcaggcaca tgtggatggc gggcgtggag acggagctgc
    15541 ggcgtctcat ctggcagatc gctgtcacag gaaaatacat ctccgccaag atccatgagt
    15601 cgaaccgcaa gctggcggga ttcaagaaca tgtacggcga ggagcaactg gccctggaca
    15661 agagttccga cgccatcctg aaaaaccagc tccaattttc cggttttgtc cgggaatatg
    15721 cctcggaaga gcaggatcag atcatccaga tcggtaaagg ccaagaaaaa tacttcgtta
    15781 ccgccgaccc cctggacgga tcgtccctgg tggacaccaa cctggccatc ggcaccatca
    15841 tcggcattca caacggcacg atgtatggaa aaggtcggga aaccatggtg gcggccctct
    15901 atatcactta cggccctctg atcaccatgg tttattccgc tggcaaggga acccatgaat
    15961 tcgtcctgaa ccgggaagga gaatatgtcc tctcccagga gaacatccgt ctccgggaaa
    16021 agggggacat ttacagtctg ggaggcctcc ggaaggactg gaccccggag catctacgtt
    16081 tcgtcgcgga tctcgaggcc gcgggataca agctaaggta cagcggcggt ttcgtcccgg
    16141 acatcaacca ggtgctgatc aaaaacggcg gtgttttcac ctaccccgcc ctgcggaaga
    16201 gcccccaggg aaaactgcgg ctcctgtttg aactgcttcc catggccttc ctcatcgaac
    16261 aggcgggggg ggaggcgaca gacggcaaac ggcgcatcct ggacatcccc gtggaggaga
    16321 tcggccagcg aagtcccatc tacatcggca gccgctatga agtggaaaag gcccggaagt
    16381 atttggagga ggtggaaaag tgatttacca gcaggttggg gaagtgcaaa aaggcatggc
    16441 cggcatcctc aagatcgccg gtgaacaggt ggagatcaac gacgggaccg gattgcggga
    16501 acgggcagtg gacgatttga tccataccgc cgtcttcagc gacgacgaag cgatgcgcac
    16561 cgcggcccgg tggctgatcc gccgggccgg cgcggcggcg ggtgtgctct cggcgtcgat
    16621 ccagcctctg tatgaggcca tgggacgcgg ggaggccaaa ggctttaccg tacccgccat
    16681 caatatccgc gccctcacct accacaccgc ccaggcggtg ctccgggccg ccatgaagct
    16741 cgacgtcggc cccgtgattt tcgagatcgc ccggtcggaa atcgattaca cccggcagcg
    16801 accaacggaa tatatcgccg ccgtcaccgc cgcggccctc aaggttggtt atcgggggcc
    16861 gctgtttctt caaggggatc atttccagat caatgcgggg aaatacgccc aaaactccga
    16921 gcgggaggtc aaggcggtga aagacctgat ctgggaggcc atcgaggcgg gattctacaa
    16981 catcgacgtg gacacctcca ccctggtgga tctcacccaa aaaacggtca aggatcagca
    17041 acggatcaac tatacccttg cggcggagct gacggccatg atccgggatc tggagccggc
    17101 gggaatcacc gtttccgttg gcggcgagat cggggaggtg ggcgggaaga acagcacggt
    17161 ggaggaactg gccgccttca tggccggtta tagggaagaa cttaagaaga tcgaccccgc
    17221 catgaagggg atcagcaaga tcagtatcca gacggggacc acccacggcg gtgtccccat
    17281 gccggacggc accgtagcca aggtgaaact tgatttcgcc accctcgagc aactctcccg
    17341 ggaagcgcgg gccacctacg gccttgccgg agcggtacaa cacggggcat cgaccctgcc
    17401 gcccgaggcc ttcgaccgat ttcccgccgt gggaaccgcg gaagtgcatc tggccacggg
    17461 gttccagaac atcgtttacg agagccccca tcttcccgcc gatctgaagt cccgggttta
    17521 tgaccatctg cgtcagacat accggggcga aaagaaggaa ggtgaaacag aggaacagtt
    17581 cctctataaa acccggaaga agggattcgg accattcaag aaggatttct gggatttgcc
    17641 cccggctacc ctggccgccc tcgggaagga gttggaaaac cagttttctt ttcttttcac
    17701 aaaattgaat atggccggga caaaggtact tctggataaa tatgttcctc cggtggatgt
    17761 ccccctgcaa gccccacggg atctggtggc atgaatccgg aaagaccggc ggcaactccg
    17821 tttggcagtg agacaccttc ccgagtaaag acaccatcac ggtgaaacgg gagtgaattt
    17881 tgttaccact ccggcgctcg gtcacccgct gccgaggctg gcgccggcaa ggcgacaact
    17941 cacaagcggc ggcggttcag cgcacctttg cccccaccac cggcgcgcga tcggcggaga
    18001 tgagagcaag accttcggcg gtatccgtgg cgagaagcat cccctgggat tcgacgccca
    18061 tgagtttcgt cggcttgaga ttggctacga caacgatcat tttaccaact atctcttccg
    18121 gcttgtaatc ctttcccatt cccgcgacga tcactcgctc ctccccaaga tcaatcttca
    18181 atttcaagag tttgcttgat ttcggcaccg gttccgcctc cagaacccgg gcgatgcgga
    18241 gatcgatctt atcaaagtct tcgtaggata tttccggttt gaataccggc gcagcttttg
    18301 tttgtgcttc cattttttct tcctttttgt attcgatacg cgggaaaagg gccgcaattt
    18361 tttgcaggac attgcccact ggcaaccctc cccattcctc cagggtggat aaatcctcac
    18421 gcgccatgtc cgttatgccc agatgggaca gcaccttttc cgccgtcccc ggcatgaagg
    18481 gggtaatgaa gacggcgatg cacctgaggg cttcaaggag attgtagatc accgtagcca
    18541 gccggtcctt caaaccgggc tccttggcca ggttccaagg ttcgctcgtc acgatatact
    18601 tgttggcccc attgacaaac tcccagatcg cgatcagggc cttgtggaat gtcaattcct
    18661 ggaagcacag ctccacctcg cgcagggtgc gccgggcgga ggtgctgaat tcccgatcct
    18721 ggtcgttcgg ggccgcgggc gccggcaccc ggccatcgaa atattttatc gccatggcca
    18781 tcacccggct gatgaggttg cccagatcat tggctaggtc ggaattgacg cgttggatga
    18841 agttttcctc actgaagcag gaatctaggc cgaagaccat gtctctcaag agaaagtaac
    18901 ggaaggcatc aagaccatat ttgtctttta aatcaagtgg tttgatcacg ttgcccaagc
    18961 tcttggacat cttactttga tcgacattcc agtagccgtg gacgtttaag tggcggtatg
    19021 gttcgattcc cgccgccttg agcatagtgg gccagtaaat gccgtggggt ttgaggatat
    19081 ccttggcgat gagatgctcc acagcgggcc agaaggtccg gaagttctcc ccgtcgggat
    19141 atccgacgcc ggtgatatag ttgatcaggg cgtcgaacca gacataggtg acataatttt
    19201 cgtcgaaggg cagggtgatg ccccaggtaa ggcggctctt cgggcgggag atgcatagat
    19261 cctccagggg ctcccgaagg aatgccaggg cttcgttgcg gtaacgctcc ggacggatga
    19321 aatccggatg ctcttctatg tatttgatca gccactcctg atatttgctc atccggaaga
    19381 aatagttact ctccttccgg tgctccggtt cgcactggtg atcgggacac aagccgtcca
    19441 caagttcctt ttccatataa aagcgttcac agcctacaca gtagaatccc tcgtagtggc
    19501 cgaaatagat atcgccggcg tcatagacct gctggaggat catccttacg acccggacat
    19561 gattttcgtc ggtggtgcgg atgaagtagt catggtcgac attcaattcc ggccagatgt
    19621 cccggaactg ggcactgatg ccgtcggcat aggtcttggg atcgacgccg gccttctggg
    19681 cggcctcggc gatcttgtcg ccgtgttcgt ccgtaccggt catgaaaaaa gtccggtagc
    19741 cggacagctt atgataacgc gccaggacat cggcgacgat ggtcgtatag gcatggccga
    19801 tgtgcggcga ggcattgaca tagtaaatcg gggtcgtgac ataataggtc ttgtccatgg
    19861 catacttacc tcgcgggggt tgaattcggt tataaatcgg cgacttttac cgtccgttcc
    19921 tcgccgtttt ccatggccac ggtgatttcg cctttcagga cgctatggcg aacgaccttg
    19981 gcccgcgtgt tgtccatttg aatgaacttg ccgcatttgg gcatgtcttt tttcatgccc
    20041 agataggttt catattcgaa ggcaaggcag cacatgagcc ggccgcagag ccccgaaatc
    20101 ttctccgggt tgagagacat actctgttcc ttggccatct tcacggacac ccggtccagg
    20161 ttgtgcagca tggtggcgca acatacctcc cggccgcaaa tccccatccc cttgatcatg
    20221 cgggtctcct gccgggcacc gatctgccgc agctcgatcc tagttttgaa ccgctgcacc
    20281 agatccttga tcagttcccg aaaatccacc cggttttcag cagtgaaata aaagaccacc
    20341 ttgccgcgat cgaaaagata ttccacatcg acgatcttca tgggaaggtc ccgctgcgtc
    20401 actttctcca cgcaaaaacg ccgggcctcc ctttccataa cctgattctc cgcctggatc
    20461 tgcaggtcct cgtcggtggc tttccggaga atgggcttgt gagcgccggt ccggcctctt
    20521 gcggacgcca attcccgctc cgcaactata gtgccgaaga ccgtaccgtt atccgtctcc
    20581 acgattacat gatcgtcctt ttgcaggggg atgccggcat catggaaata atatacctta
    20641 ccctcccgct tgaacttcac gcccaccaaa tactcttcag acatacccac cctaaaaatg
    20701 catcagtagt ttcccaatgt tattgagtaa cttcctctga aatccataga ttgcaatggc
    20761 ttccatagtt gttttgattt aaaggcggag tgtaaacgtc atggcctcta atgtcaataa
    20821 tttattggcg ttggcttcta gagcccttag ggaagcggcg atggcatcca gattgaagag
    20881 caggtcatcc gtggtcagcc tggcgatgcc gtggacctga tccaggtgat cgccgtggac
    20941 gagcattccc tggtcccgga gttccttcca gaccaaagca tcccgaaaac aggattgcag
    21001 aagggtaagc ttgtcgtgga tttccttctt gctttttccc agatacctga acagcgccag
    21061 tctcggcagg ggggcaccgg catgcccgga ggcgaggcgc gtcaagatcc cttcccggtc
    21121 cgccagataa tcctcctgct gaagctggag cgctcgggcg atgcttccct cggcactggc
    21181 ggcaagggcg cgggtgacgg tggcttcgag accggcttgt tcttccagga aacgggcgac
    21241 ggtttcccgg cgcaggggcc ggaagcggag ctgctggcag cgggagatga tggtccgggg
    21301 caaaaggtaa ggcttggcgg tgatcagaat gataatattc gccgccgccg gttcttccag
    21361 ggttttcagc agggcgttgg ccgccgccgg gtgcaaccgg tcggcatcgt cgataagaaa
    21421 caaacgccgc cctccttcca tgggtcggaa ggccatgatg ccgatcagtt cccggacggc
    21481 ctgcattttg atgaacgccc cgtcgggctt gacggtgatc agatcggggt gattgccgct
    21541 tttcaccttc ttgcaggacc ggcaggcatt gcaggcctcc ggtcgcccct tcccgcaaag
    21601 gacggccgtg gcaaaggctt tcgccacctc cttcttgccg attccgtcgc ggccgtgaaa
    21661 gagataggca tgggccaggc gcccgctggc aatggccgct cccagttgtt ccttttgccg
    21721 ttcatgtccg tagacacccc ttccggacat ccttctcaac cccattctgt cgctatgtat
    21781 ttaatccccg ctccatcagc atcctgtcca ggtggaagca aactgtttcc cgcagctcgg
    21841 caatggtgcc ggcggcgtcg ataacccgga accgctccac ctcgcggtgg gccgcaagca
    21901 ggtagccttc ccggaccttc ctgtggaaag acaattcttc cgcttcgaaa cggtcttcct
    21961 tttgcccggg ggtcatgagt tcaatgcggg tccgggcccg tgtcagcccc gtctcgactg
    22021 gcaaatcgaa aagcagggtg agatccggat gtcgcccctg gatggaaaag gcattgagga
    22081 aattgacggt ttccatatcc agcccccggg cgtaaccctg atacaccgcg gtggcatcgg
    22141 caaaccgatc gcagagcacc accttgccgg ccgccagggc cggcaggatc gcctcgcgga
    22201 catgctgcgc ccgggcggcg gcaaacaaca gcagttcggc ttcaggacaa atagcgaatg
    22261 gcccccgatt gaggagcagt tcccggagcc gcctgcccaa agaagtgccg cccggctctt
    22321 ccgtccggag gcacggtatc ccccgctgac cgagatactc cccggccagt tcgatttgtg
    22381 tggttttacc actgccctct atgccttcga aggtaacgaa tagtcccatg acgtattaaa
    22441 tactgggagg ggtaaagaag gggtgaccct ggccgcttac ttgtcgtccc cggggatgat
    22501 cgcttccacc ggacattgat ccgcacacgc cccgcagtcc gtacaaacct tgggatcgat
    22561 tacatattta tcctcgccct cgctgatggc ctctaccggg cactcgtctt cacaggaacc
    22621 gcaagcaatg cattcgtctg taatgatata cgccattttt ttcccaatct ccttttaatt
    22681 tcatttcatt tgttgactta tgccgacact acctcccgga cctccgggac ctcctttttc
    22741 aggtagcgtt cgataccgtt tttcagggtc atctgcgcca tcgggcagcc atgacaggcc
    22801 cccaacagcc tgaccttcac caggccatca tccgtcacct cgagcaactg aacgtcgccg
    22861 ccatccgcct gcagattggg acgaatcctg tcgatggccc gctgaacctt tgctttcata
    22921 attacctccc cggtggttgc catcaatgag gcatggcgcc gatcattgtc tcgaccacgt
    22981 ttcttgccag atctttcaat tccggtttca aaaaagtata ttccttcacg acatcatggc
    23041 cggcccgcca ggcggtcttg ccggtctggg catcgatcag cctcatgctc aatccggccc
    23101 gggccacctt ttccttgttt tccacggcat aactccagga atcgacggtg acgacgagaa
    23161 atgcgtccat gttcaaggcg acgccaatct tcatactcag ggccggatcg gagtagttca
    23221 ccgttttcag cttcgccaga taatcgttaa ccgcctgctg caatacctgg tcgccctgaa
    23281 gcctcgcctt cagggtggcg gcgggcagga cggcggcaaa ccagtctttg tttgtgagcg
    23341 cggcgctgac cagctcttcg actatccccc tggcctcatc ataacccccg gcatcgacgg
    23401 gcaggacacc aatggaccgg gggtgaaaat ccggcaggtc cggatcccgc tccacatatc
    23461 ggagggaacc gccgcaggca aggataatca tgatcaccca tcccaggatc aaaaaacacc
    23521 accatgattt tgtgaacatc aatcgcctgc gacgatttgt catcataagc ccttcccccc
    23581 gaagatcaaa taccacaccc aggagctcaa accggctatt atataagcgg tttccagtac
    23641 accctccttc accaccccga taagacccgg ttttttatca taaagccgga agcaaatcca
    23701 tatataaatt acggggatga tcaggaaata acttagcgat acggtccgga ccagggtggg
    23761 agcggcggcc agcatcatga caagcgccag ggaaatcata ttcagcagtt gctgggtgtg
    23821 gtcattgccg atcaagacgg ggatagtttc cttgccgacc agcttgtcgc tctggacgcc
    23881 caggaggtcg gacagcaccg agcggacaaa gacaataccg aagataaagg caaaggaaac
    23941 cgccgtggtc tcggtaaggg gcaatttttc ggccaccggc ggcagaacgg cggtgatagc
    24001 cgcccaggcc aaggcaacgg ttatgttttt cgatccgggt agatccctga tgctgcggaa
    24061 aggccagccg gacggcgcca gttgggcatt gtagaggata ccaagggtgg acattgccag
    24121 taggaataga aaggccgtcc ttccggacaa taaaccggcc cccagggcaa acaccagggc
    24181 caggatggca gcggcgagat acttactttc gtgacggcgg taggattcct cgcggaaaga
    24241 gccgataatg cttcccgttt tccggttgat gaaccggttg agggtatgca tggcataaac
    24301 atacagcgag gctacagcaa tgtagagaaa ccgtagcgtc aactcctgga agagcatggc
    24361 ggttgcggcc agacaccccg ctcctagggc ggaataaagg tccgtccgga cgctccagac
    24421 ccaaaatcta aagatggtgt tgatttgccg ggatttatcg gcctgcctgg tggtaacggt
    24481 gtcgacgacc cggtcaataa tccaattcgg cgtggaggcc ccggcggata tcccgatctt
    24541 ttcatatgcc gccaattgtt cgaccgggag ttcctcggag gtttccacat gaaaggtcgg
    24601 cttccccggc aacgccgcca ggtcggccag acgttgagtg ttggcgctgt tccgaccgcc
    24661 cacgataatc atggcatcca tttcctccgc cagggatttg acctcgctct gccgtctctc
    24721 cgtggagtcg cagatcgtat cgaagatgac ggcgtcggga aagcgcgcct ggatcgccgt
    24781 gacgatgcgg cggtattcat ccacgctctg ggtggtctgg gcgacgacgc acaccttgtc
    24841 caggggcggc aaccggtgga catcttccac ggcgcccagc agaagtccct tcccgaaggt
    24901 atatcccagc agtccgttca cctcgggatg gccggcatcg ccaatgatga ggatggtgta
    24961 attccggacg gcatgctttt tgataatggc ctggacacgc ccgaccttgg gacaggtggc
    25021 atcgataatc ttgaggtttt tttcccggag cagctttcta tcctgggggg aaatgccgtg
    25081 ggcgcgaatg atgatgccgg ccgctttcgt gccggcgggg atttcctcga tggcggcaat
    25141 gggaacgatc ccccgttttc tcagcaattc cacggtctgg gggttgtgga tcaggggccc
    25201 gtaggtatag atggtctcct ggcccttgtg ctgggccaac tccaggacca tgtccacggc
    25261 ccgcttgacc cccatgcaga agcccgccgt ttccgcgagt ttgacgctca tgaccttttc
    25321 gccgcgtaaa gaaagaactc gcggtttccc gccggtccca ggagaggaga gggacaggtt
    25381 cccagcaccg ccatacccag ggtccgcaca aaggtctgta gatccccgac tacttgccgg
    25441 tgtagatctg gatccgacac cacgccgttt ttgcccacca tgccctttcc gacctcgaac
    25501 tggggtttga tcagggccag gatcgcgcct tccccctcga tcaggggaca aaccgcgggc
    25561 aggaccaact tcagggaaat gaacgaggcg tccacggtgg ccatgtcaac tatgtccagt
    25621 aaatcccggc cgtcatagta acggatgttg gtgcgctcga tgggcacgac ccggggatcc
    25681 tggcgcaacc gccaggccaa ctgcccgtag cccacatcca gggcgtaaac cttcttcgcc
    25741 ccctcttgga ggagacaatc ggtaaaccct cccgtggagg ccccgacatc gataataacc
    25801 cgatcccgta ccgatatggc aagggcatcc agggccccct tgagcttccg tcctccccga
    25861 cttacatatg gttccgcctc tctctggatg cgcagttcca cccggggatc gatcagggtt
    25921 ccggctttat ccactcgctt ttcatccgcc agaacggccc cggtcatgat catcgcccgg
    25981 gccttctccc ggctgggtac caattgacgt tccaccaaga ggacgtccag gcgtactttt
    26041 cccatggcaa caccctgacg atcaaaccgc ggctgaagat gtagatgaca attcatccgc
    26101 ccgtgccgct tccataccac ctcactcctg ccggccgctg ttgcctgctt tttgcggatc
    26161 cggaacaaac ggtccgccgg gggtaacttc agcatgccac cgtcgcgcgt tgcccgcttt
    26221 ccgagatcag ttcccgggcg gcggcaagga tatggttgcg gtcgatgccc gcctggcgtc
    26281 gcagctcctg ctgggtggca tgttccacaa agacatcggg gatacccaat atcctgatcc
    26341 gcacatcact catttcttcc acagccagca gttccatgac cgcgctgccg aatcctccca
    26401 tgcgaacgtt ttcctctacg gtaatcaact tcttgaattt tccggccgtt tcacgaatca
    26461 actctcggtc caggggtttg acaaagcggg cattgattac ccggatggcg attccctcct
    26521 tacggagctc ctccgcggcc gccagcgccg gatagacggt agcgccgatg gcaatgacgg
    26581 ccaactcatt gccgtcacaa agtatttctc cacggccaat ggccaggggg tgtggctcgc
    26641 aatcaagcgg caccccctgg ccattgcccc ggggatatct gaccgccacg gggccgccgg
    26701 catccaccgc tgttttgagc atatgctgga gttcgttttc atccttgggc gccatgacca
    26761 tcatgttggg gatggacctt agatagctga ggtcgaagac accgtgatga gtggccccgt
    26821 cgtcgcccac aaatccgccc cggtcaaggc agaagaccac atggagattc tgcaggcaca
    26881 catcatggat gatctgatcg taggcccgct gtaagaaggt ggagtagatg gccaccacgg
    26941 ggatcagccc ttccaccgcc agaccggcgg cgaaggtgac gctgtgctgc tccgcgatgc
    27001 cgacatcgaa aaagcggtcg ggataggccc gggagaattc atccaggccg gtgccgttgc
    27061 acatggcggc cgtgatggcc acgatgcggg agttttcccg gccgagccgg agcagggtct
    27121 cgccgaacat cccggtgtag gtaatcgggg cggatccgtt ccccccgccg gtgggccgcc
    27181 ccgtctcgat acagaacgag gaggtgccat gaaatcgcag aggtttttcc tcggcatgct
    27241 gatacccctt gccctttttc gtcaggacat gcaccagtat tgggcccttc atttgcctga
    27301 tattgtggag atttttaatt aaacggtcca aacggtgccc ctgcaggggc cctacataga
    27361 tgaaacccag atcctcgaaa agggcgccgg gcagaaggaa ggacttaagg gcctcttcga
    27421 actgtcggga gaatttcacc atgtggccgc cgatggaggg aatgctgctg agaaatgtct
    27481 tgagatcggc ccggaggcgg gtcaccgtct cccccgtcat gatccggttc agatacgaag
    27541 acagggcgcc cacattgggt gagatcgaca tctcgttgtc gttgaggatg atgatcaggt
    27601 cttccttgcg acccccggcc cagttcaggc cttccagggc catccccgcc gacatggcgc
    27661 cgtcgccgat aaccgcgacg atcctgtggt tttccccctt gaggcgccgg gcttccgcca
    27721 tccccgtcgc cgcggaaatg gaggtgctgc tgtgacccac attgaagaca tcgtaaatgc
    27781 tttcctcacg cttcggaaac ccgctgatgc ctccctgctg ccgcagggtg gaaaaggcct
    27841 cccggcggcc ggttatgatc ttgtgggcat aggcctgatg tcccacatcc cagactatct
    27901 tgtcccgagg ggtgtcgaag acgtaatgaa gcgccagggt cagctccacg gctcccagcg
    27961 aggaggcgag gtgaccgccg gtccgggcca ccgtcgttat gatcaactcc cggatatcct
    28021 gagcaagcaa gctcagggag gggatatcga gttgccggat atccttaggt tcgttgatgc
    28081 cagcaagtat gtttcccact taagtatttc tttccataat gtatttggca atctgtacca
    28141 ggggttcgca ccgttccccc agccgccggg cgtccgccgc cgccccgtca accaggtcag
    28201 ccagctttgt ccgggaggcc tccagcccca gcagggccgg aaaagtctgt tttccccgga
    28261 cggcgtcgct gcccgtactt tttcccatca tgacgcggtc gccggtgaca tttaaaatat
    28321 catcggcaat ctgaaatgca agtccgatct tttcaccgta accggcaagg atttccttct
    28381 gtccatccga agcgccagcg atgacggccc cggcaacgac ggaaacgacg aggagcgccc
    28441 cggtcttgcg ccggtgcata tccgtgaggg ttggcagctc caggtcctgg ccctcaccct
    28501 gcagatccat cacctgacca cccaccatgc ccgccatgcc cgctgcccgg gctaccagcc
    28561 ggatcacctc caggcggtct tccgggacca caccgggcat taaatcccgg cgggccagga
    28621 ggtgaaaggc ctcggtcagc agggcgtccc ccgccaggat cgccagcccc tcgccgaaaa
    28681 cgatatgatt cgtcggccgg cctcgccggt aatcgtcgtc gtccatagcc ggcaggtcat
    28741 cgtgaatcag ggagtaggta tggatcatct ccaaggcaca ggcgcaggga agggcatccg
    28801 accaggcgcc gcttaccacc tcggcggcgg ccaggcagag gatggggcgg agccgcttgc
    28861 cgcccgcaaa gaggctgtag cgggcggcgt ccattatccg ttccggcacg ccggtctccg
    28921 gggagagaaa gcggtccagg gcctcctcca ctaaggcctt gcgccgggcc agatagacat
    28981 ccaaatcacc gtatgccgtt tccgccgcca tgtcagccct ccgtagggaa ggggacggcg
    29041 gcaaggcggt cgtcgtcgtc ccggagcagg atctcaatct ttctttccgc ctcgtcgagt
    29101 ttccgggtgc aaaaacggat cagccggatc ccttcctcga aggccttgag ggattcatcc
    29161 agggtaagat cgccggcttc catggttttt accagctctt ctagcttctt taaggcctct
    29221 tcaaactttt cttttgccac gttcacctct cccgccgctc accgcgcaac cgcggcccgg
    29281 tcttttccat ctgttttccc atgcgcagtc ctgaaaacat tatttcgaaa aacataattc
    29341 ttatcatctc ccctcccggg tatgtccctt ccccgccggc gagggaggaa gttgtatcgg
    29401 cagcagggat cagaaaagcg atccgctctc cttcatctcc tcgggagcca ccacctccac
    29461 gcgggcgcgg atttcccctg ccgccaaccg aacggaaagc cgatctccgg tggcaaggtc
    29521 ggcggccctt ttcacgatca ggccgtcggg gaaccggcgg gtgatgctgt aaccccgccg
    29581 caggacttgt agggggttta gcgcctgtag gctgacgccc tgccggtcca tgacggcccg
    29641 ctgctgccgg aaaaaggcgc cgatccccgt ggcaaactgc tcccgccgat gggcgagaac
    29701 cacccggata cggtcgatgg ttccggtcgg acagacatgc tccaagcggg tccgcagata
    29761 ctgcaggcaa tggcgccggg agataatgtg ccggttcagg gccaggaaaa gccgctcccg
    29821 atgatcgtcg aggaggagcc gataatcggc aaggatgcga tccggcgact tgagctgtcc
    29881 cgccgccgct tcgatccgtt cccgccagcg atccacccag cgctgccggg cttggagcag
    29941 acttccatat gcatcaccga cctgccggcg caagtgctca cggtcgggaa ctaccatttc
    30001 cgccgccgcc gagggggtgg gtgcccgtag gtcggcgacg aaatccgtga tggtataatc
    30061 tgtctcatgg cccacggcgg aaacgacggg gagacgcgac cggaacaccg cccgcgccac
    30121 ctcttccgta ttgaagggat ataggtcctc cagggaacca ccgccccgcg cgatgatgat
    30181 gacctcgaga tccggataag caccgggctc cccgctttcc ccgttgagca gggcgagggc
    30241 acggacgatc tccggcgccg cctcgggtcc ctgaacccgc accggagcga tcagaatggg
    30301 gacggatggg aaccgccgcc ccgtaatttg catgatgtcc cggatcaccg ccccggtggg
    30361 cgaggtaatg acgccgatgc cccggggcag aaagggg
//
LOCUS       DAOSMF010000015        28077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_2183_length_28077_cov_22.297730, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000015 DAOSMF010000000
VERSION     DAOSMF010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 28077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28077
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2183_length_28077_cov_22.297730"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1182
                     /locus_tag="PLT63_03355"
     CDS             <1..1182
                     /locus_tag="PLT63_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase"
                     /protein_id="HOD97791.1"
                     /translation="AIAAFHDDRLYVEKYIEKPRHIEVQILADQYGHVIHLGTRDCSI
                     QRRHQKLLEIAPADLPAPVLEAVHAAAVRAARAAGYVSAGTVEFLVDSRTNEFWFMEV
                     NTRLQVEHTVTEMITGEDLVQEQIRIAEGRPLQILQEDVRLHGIAVEVRINAEDPKNN
                     FLPEGGKLIEIYHPPGGPGIRIDGNAYKGYLVPTVYDSLLAKLIVWGYEWEQTVSRLQ
                     RALKDFNIVWPKTTIPLYMAICDEDDFRHRRFHTGYLDEHPELFKYPDMEHFVLDDAL
                     YKLMLAPHEGMEGRSGEERKKDRQPGWWMNKRILTAQEIEELKKEGIVKSSIKGKVTD
                     IPVEVGDEVMVGDVLVDLEAMKMHHHVICEVDGKVADILVEPGDAVDVGDTLVIVNVR
                     G"
     gene            complement(1385..2887)
                     /locus_tag="PLT63_03360"
     CDS             complement(1385..2887)
                     /locus_tag="PLT63_03360"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOD97792.1"
                     /translation="MEDTENLTKTIPSSPVPEAEGSDAGGGADSLTSERRQSTRRRRP
                     SRKGKKRPEAIDAGAVPEPAPVTDDSWDPRQFKVPAAPGKTRFQDFDLPAPLLHTISD
                     LGFQYCTPIQAEILPSALSGKDASGRAQTGTGKTAAFLIAIITRLINHPHTEERPFGT
                     PRALILAPTRELALQISAEARQLAKYCGIRIVSVFGGMDYEKQRHQLAAGPADIIVAT
                     PGRLLDFQRRGDVVLKKIEVLVIDEADRMLDMGFIPDVRMIIHSTPPKEKRQTLMFSA
                     TLTGTIIRLASSWTVDPVMVEIEPEQVAVDTVEQIVYIVTEEQKFDLLHNIIVRQDLE
                     RVLVFCNRRDEVRRLAERLTRYGVNCSELSGDIPQKSRIRRLEDFKSGKIRVLVATDV
                     AGRGIHIESMDHVINFTLPHDPEDYVHRIGRTGRAGQSGTAVSFADEGDAFYIAPIEE
                     FMGRRLPCIEPDEEMLVKPEALYPEKKVRPRSTRRPGGGKSRPRPRRPHF"
     gene            3133..4386
                     /locus_tag="PLT63_03365"
     CDS             3133..4386
                     /locus_tag="PLT63_03365"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOD97793.1"
                     /translation="MKIEICAASGCARAIIMITFVVGCLLSGCAAGPPRLQPSGLNTV
                     FAGKDGMPFADYIKQTRAMMLRARVDINDGNREMVLNANLPFELAPDTNLFPLGKDGK
                     HRRGALLVHGLSDSPYMLQPLARHLRNRGFLVRTILLPGHGTAPGDLLEVHWQEWRRA
                     VQYGIDGMKGEVEELYLGGFSTGAALGVLSVLSGEDIKGLILISPALGVKDRRVALAG
                     ALRSVRDWVGDIRDDVDYAKYETFAVNAAYQIYLLTGEIDRLLAAGKRIEVPVFAALS
                     AEDVTVDAGRAQEVFRRYTTSEHNVVILYARDPRPEICAGSGTIYCEGSYLPELRILD
                     FSHVALPMPCDDPHYGLRGGYKSCLHYGADREKRGICRNDDTIWQGEISDANLQSFTL
                     RRLTCNPKFPGMRERLDQFIRNVAR"
     gene            complement(4367..6646)
                     /locus_tag="PLT63_03370"
     CDS             complement(4367..6646)
                     /locus_tag="PLT63_03370"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOD97794.1"
                     /translation="MTGAEEPRPLRIGLVLSGGGARGIAHIGVLKVLEEMRVPIHCIA
                     GTSMGAIVGGLYAAGSSPEELEKLVTGIPWHEAFTDKQTVDKLSFRRKQDSMDYKIDF
                     NLGVRNGRLATSRGLVQGQNLNILLKKLLLHTADIKDFDHLKIPFRAVAADIETGDTV
                     VLGKGDLAEALRASMSIPGIFVPMEINGRMLVDGGIANNLPVSVARAMGADIIIVVDI
                     GTPLRTREGLNSTASITAQVMTILIQRNVQAQLRTLKPDDILIQPDLGPLGTTDFSKT
                     ETAMKIGAAAVGKMKERLAGLSIAPEKFQVYLAHQRRQPSEPPVIDYVKVEFKSASPA
                     QASPLPSVKTGSEGKEIDLGSKLAHLPGVKTRAENRTKISPRVLEAQIETKAGEKLNI
                     ETLEKDITRLYGLDTFERVDFHLERRRNKTGLVFNPIEKSWGPTYMRFNISLADNFKG
                     SSSYTVGTRITQTEINTLGAEWRNELQIGETTRLFSEFYQPIDYSTHYFFAPRLEYRE
                     WNAYIYSPEYTADIMAKYRLKEFSGGLDLGRQFGNWGELRLGIRRGYGAYRVNVGAAD
                     LPTDSFNIGGFFSSLTYDTMDNFNFPQRGLSANVTSQFNLPEMGSDYKVAGINANIQG
                     AKTWGRLTFIPGIIYGGTLDGDSKIQDSYSIGGFLNLSGYLTDELSGQEVGLARIVCF
                     RNMGAAGLGDFRTTLYLGFSVEAGKAWQKRSDITLGSLIYAGSAFIGADTFIGPAYIA
                     YGFAEGGHQTLGLFIGQRF"
     gene            complement(6835..7206)
                     /locus_tag="PLT63_03375"
     CDS             complement(6835..7206)
                     /locus_tag="PLT63_03375"
                     /inference="COORDINATES: protein motif:HMM:NF012305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97795.1"
                     /translation="MVKKLYIGNLAEGVTEDDLMANFSEIGKVVSLAIIRDKITGLSR
                     GFGFVEMATEAQAQEAIQTFNGGELLGRTIRVDEAKPKGDQDERPRGTGSRHGGFSSQ
                     APRRGGGPRRSGGGGREGRQF"
     gene            7587..9818
                     /locus_tag="PLT63_03380"
     CDS             7587..9818
                     /locus_tag="PLT63_03380"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HOD97796.1"
                     /translation="MKKKTSPSAIPCLTKNAITVLERRYLRRHTDGRVLETPAHMFRR
                     VAESIAAAERIFSPQADTGKLVEKFYRIMADLEFLPNSPTLMNAGRELGQLSACFVLP
                     VGDSMEEIFDAVKYTALIHKSGGGTGFSFSRLRPANDVVMTTTGISSGPISFMRVFDI
                     ATETIKQGGTRRGANMGILRVDHPDIMDFIMCKADKNQLNNFNISVGLTEVFMAAVEA
                     DENYDLINPRNGQVTGTLNARKVFNRIVVQAWENGEPGIVFLDRLNRDNPTPDVGAIE
                     STNPCGEQPLLPYESCNLGSINLGKMVTGGAVDWKRLREVVHLAVHFLDNVVEVNRYP
                     LPVIAEMTFGNRKIGLGVMGWADMLIMLGIPYDSEEAVTLAGQVMKFIKEEGHNASRH
                     LAKQRGPFPNFSASLYAKKGEPPLRNATITTIAPTGTISIIANASSGVEPIFAVSYIR
                     QVMDDDILVEVHPLFEKTARQRGFYTPELMKRIAEQGTLHDLEELPADIRRLFVTAHD
                     ISPEVHIGMQAAFQKYTDNAVSKTVNFPRDASVEEVAKVYRLAYQLDCKGVTIYRDGS
                     RDKQVLSRGKGEEKTARIGGDEERKVVKRERPKVLKGWTYQMQTGCGPLYVTVNQDST
                     GLFELFTTMGKAGGCAASQNEAIGRMVSLAWRSGIQARQVIKQLQGISCHSPSGFGEN
                     KILSCADAVAKAIQSHMAANGYETVREKVALVKGACPECGGIVSHEGGCAVCRVCGYS
                     ECA"
     gene            complement(9868..10890)
                     /locus_tag="PLT63_03385"
     CDS             complement(9868..10890)
                     /locus_tag="PLT63_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97797.1"
                     /translation="MSSPSSREPSVPIGKNRANFTREFLFADAGIVRDHDGVHTAMTM
                     DERLEGWRRIPHGGIAMGAVMELAALLREEWTEKDRGPEYPLTVDFRLGGAGVRIGDK
                     VEFTVLAAPEGIRGTIFTPGNNDPYLSAVIGYGIDDPQRRETFQSYLPATISNPAEGG
                     IPLPYYRNCFVCGVARRQPGLERRFLLGGGDGGTPRLAYAAAGFDVTDENTFYRFQSG
                     GRLSLLPSLALLDETMGWAGFMMAASGAVTVRIGYTFYRDIRPGERLVFIGRSERVRG
                     NAGARIFFWTSGGAVSLDNAGRCEPVIAASAQYLGVAELTEQMRRELIPAELTAQAFR
                     LAGLTH"
     gene            11195..13891
                     /gene="fdhF"
                     /locus_tag="PLT63_03390"
     CDS             11195..13891
                     /gene="fdhF"
                     /locus_tag="PLT63_03390"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015903581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:12251..12253,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HOD97798.1"
                     /translation="MKDAVFSLNGKETVYQPGQSVLEAARAAGVNIPTLCYLKDTTPT
                     GACRICLVEIAGARTLMAACTTPVAAGMEVFTETERVHKTRKLNVELLLSSGRHDCLV
                     CEKNGDCRLQDLAYYYGIKEMRFPQDQDFYPTEEENPFIVRDFTRCIRCGRCIQACNE
                     LVVNRAISYGYRGKDSKVVTGGDGPYLGSECVFCGQCVEVCPVGALTEKKARGKARAW
                     ETKKIRTTCAYCGVGCQQWLHVKDGRITKVTAVEDGEPNMGRLCVKGRFGYDFIYSED
                     RLKTPLIRENGNFREASWDEALDLVAGKFKEIIAKDGPDAIAGVSCARSINEDSYQMQ
                     KLFRAVLGTNNIDHCARTUHAPTVAGLATSFGSGAMTNSFSDFAKAKMILVIGSNMTE
                     AHPVAASLLKMAVIGGAEMIVVDPRRTPLVDLANLHVQIKVGSDVAFLNGVMNVLITE
                     DLYDKNFVESCTMGFEELKQKVLEYPPERAAAISGVGADVIRTVARRLAANRPAMLMY
                     TLGITEHICGVNNVMSCANLQMLLGNVGFECGGVNPLRGQNNVQGACDMGALPNVFPG
                     YQRVDNPQAREKFAAAWKVEKLPEKPGLMIPQMIDGLLDGKVKAFYVFGENLANTEPD
                     IRHVENCLATAEFLVCQDIFPTETTRFAHVVLPAAAWSENDGTFTSSERRVNRVRTAS
                     VPPGEAKPNWWIFKEIARRMGYVWESDSAQELWDNEVSQLAPSMAGIKYFRLEGNGLQ
                     WPAPSLESEGTSYLHKDGCFTCGLGVFKAIDWIPPAEIPDGEYPLVLSTGRRLYHYHT
                     RTQTGRSDGLNVLLSEETADISEADADKYGIADGEMIAVKSRRGRVAVKAKITPQVPP
                     GMVWMAFHFREGCANWLTNPVYDPITQTAEYKACAVAVEKIT"
     gene            complement(13945..14313)
                     /locus_tag="PLT63_03395"
     CDS             complement(13945..14313)
                     /locus_tag="PLT63_03395"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOD97799.1"
                     /translation="MEIKYKIWLEKDGKVIFGHGREELLQAVAECHSLNAAAKKLNMS
                     YRAAWGRLRASEDRLGIKLIESDAAKKGMTLTPAAMTLLEKFRRLEKDADSYLKKTSR
                     ELTTLLDGEKRPDQENKKGK"
     gene            complement(14391..14957)
                     /locus_tag="PLT63_03400"
     CDS             complement(14391..14957)
                     /locus_tag="PLT63_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit E
                     family protein"
                     /protein_id="HOD97800.1"
                     /translation="MKFGSYSYDEYIHLVKSFHGNLAPGLIIGGFIVETALKNLPEGE
                     FFDAVCETSVCLPDAVQLLTPCTIGNGWLKVFDLGRFAVTLYEKESGKGIRVYLDTEK
                     LKSWPEFHAWFFKLKPKREQDLETLLNQIKEAGASVLSMQEVRVKPEITVKRRLGSTA
                     VCPQCGEAYPLRDGASCLACQGKSPYVS"
     gene            15397..16287
                     /locus_tag="PLT63_03405"
     CDS             15397..16287
                     /locus_tag="PLT63_03405"
                     /inference="COORDINATES: protein motif:HMM:NF016131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HOD97801.1"
                     /translation="MRDAEKTEENLREIMAREIAGNPHAEAIIGAFQPLLLARRRLLA
                     AEKWPAVTLTGFDPARFGAGIPLLRQVQFFSAADPWGEMTDMMAAAIGAGFPVLNRDM
                     ERIAGAARNKDIDLFSYFHKLVDAGRDENLVFSWAEKLSVSAPVLEFLLRQLSRVVLE
                     KRLTEAAALIGDIEWRHGYCPVCGEFPSIALIEEKITRRWLHCSGCGHEWIFSRVICP
                     YCEHEAQQGMDFFYVEDRPRETAFSCDQCRRYLVTLKGVSELADRDLDVTAMSLTHLD
                     VIMQGKEFSPMVNTPWNALR"
     gene            16750..19815
                     /gene="fdnG"
                     /locus_tag="PLT63_03410"
     CDS             16750..19815
                     /gene="fdnG"
                     /locus_tag="PLT63_03410"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:17335..17337,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HOD97802.1"
                     /translation="MEITRRGFLKVSGAVGSGIALSGLGIDLRPVHAYADELDKMNRV
                     KMAKQVSTICAYCSCGCGLICSVDKLTGKIFNIEGDADHPINEGTLCAKGAGFFDLTE
                     ANKHRLKKVLYRAPYATEWEEKDWDFAIPRIARLIKDTRDKEFITKNAKGELVNRCET
                     IGHYGSSNVDNEECWLMSVKARALGMVYIDHQARVUHSPSVAALGESFGRGSMTNHYV
                     DMQNSDCILIMGSNAAEHHPMAFRWMLKAKERGAKLIHVDPRYTRTSARCDYHVPLRS
                     GTDIAFLGGMIHYILENDKWFKEYVLQYTNGSFIVGPNFKFEDGLFSGYDAEKRKYSK
                     DTWVLEKEGSGIPKRDMSLSDPRCVFQQLKTHYSRYTLDKVSSITGVSKENLLKVYAE
                     FSATGVPDKAGTECYALGWTHHTTGSQIIRTMAIIQLLLGNMGIAGGGINAMRGEPNV
                     QGSTDQCILYGNLPGYLKMPSASLESLDHYLEKYTPVSKDPRSANYYSNYPKFFVSFL
                     MSLFDNKATKENGFGYNWLPKMDDGKHYSTMHMFDAMYAGKVKGYFCVGADTAVSTPN
                     SKKVRKAMENLDWLVGENIFNNETYEFWRGPGVDPKKIKTECFLLPAAATMEKEGSQS
                     NSGRMIQWKYQAAPAPGDGLPVGEITIKIMKAVKELYAKEGGPNAEPILNLYWDYLDG
                     AGHFDPLKVAKRLNGVFLKDTVIEDKAKGTKVEFKKGQCVPGFGNLQLDGSTACGNWI
                     HSGSFTADGKNNMARRGKDDPTGLGIFPNWAFVWPVNRRVMYNRASCDINGKPWDPKR
                     KVLEWKDGKWVGDVPDGPWPPLGLEGGKYPFIMQKDGMGAIFGPGMVEGPFPEHYEPL
                     EGPLAKNPMSSQLINPAIEIFKSDLDTIARASEKYPYVCTTYSCTEHWCTGALTRWQA
                     WLLEAMPEAYAEIGETLAKEKGIRNGERIKISSIRGDVKCVAMVTKRFKPFQVEGKTI
                     HQIGLPFNYGWLFPKDTKDDSTNFLTPTVGDANTFCPEYKAFMVNVEKL"
     gene            19829..20626
                     /locus_tag="PLT63_03415"
     CDS             19829..20626
                     /locus_tag="PLT63_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOD97803.1"
                     /translation="MNNKREVVKLIDTTLCTACRGCQVACKQWNEMPGLPTKQWGSYQ
                     NPPDLEWNTWTLIRFQEHVNEKKEFKWLFRKDGCMHCTDAACVKVCPSGALHHTDFGT
                     VGLNSSLCIGCKECVSACPFDVPRYNRETDRIYKCDLCYSRLRGGESPACVKSCLTGA
                     LDIGPKDKMLKKAYAKVKQLGGDANVYGDKFVGGTHVIYVLDEKPKVYEKLPRNPSVP
                     FTVTIWKDILKPLSLLAAGGVIGGSFVHYILHGPKLPDEPGQEKGGE"
     gene            20630..21298
                     /locus_tag="PLT63_03420"
     CDS             20630..21298
                     /locus_tag="PLT63_03420"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HOD97804.1"
                     /translation="MRKGLIQATDGFERIVHWSLALSCLALCVTGLGMMFHSLNFIGE
                     LLGGLKSLKYVHNFTGIFFGVALYFTIRMWWKEAGVFSMPEDLEWIKCAGGYLWHVDN
                     PPEVGKYNPGQKMFFLAVAGFGVIMFLTGLVMWFPLSFPPTFVRLMYVLHALGFVVIF
                     AFFFVHLYLGTVGSPGSLPAMLTGWVTREWLKKQHPKWLKEMEHEGKLVVYGEEKPSG
                     GHGH"
     gene            21889..23640
                     /locus_tag="PLT63_03425"
     CDS             21889..23640
                     /locus_tag="PLT63_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOD97805.1"
                     /translation="MSLVTVTIDNRQVQVEAGTTILEAAKRLGIKIPTLCAWAEIGHA
                     PGACRVCLTEVEGQRSLTAACSFPVTEGMVVRTNTERVRVARRLVVELLLSNHPQECN
                     FCVRNGNCELQRVAEAAGIKRLRFEPPEFSREGMIDYSSPSLVRDSRKCIHCHRCVTV
                     CEQIQTVGVLTPSFRGSDVRVAPAFGLPLDESNCIACGQCVMACPVGALYEKDDVDAV
                     WQAINDPSKHVVVQEAPAIRSALGEEFGMPPGSLVTGKMVTALRRLGFDKVFDTNFTA
                     DLTIIEEGHELLKRVKEGGVLPMITSCSPGWIKFCEHFYPDLLPHLSTCKSPQQMFGA
                     LAKTYYAAEAGINPQDIVSVSIMPCTAKKYEAGRPEMTSSGYRDVDYVLTTRELARMI
                     KEAGIDFVNLPDGEHDAPMGEYTGAGTIFGATGGVMEAALRTVYAVVTGQNLPDLNIT
                     TVRGLTGVKEASVEVGPLGKVKIAVTHGLGNARRLLELIQAGKSDYAFIEIMCCPGGC
                     IAGGGEPIPTTDEIRTLRAEALYRDDGTVQKKRQSHENESVQNLYRKFLHEPLGHKSH
                     ELLHTHYVRRGENVPHK"
     gene            23701..24978
                     /gene="hydF"
                     /locus_tag="PLT63_03430"
     CDS             23701..24978
                     /gene="hydF"
                     /locus_tag="PLT63_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HOD97806.1"
                     /translation="MNETPKGNRTHIALVGRTNVGKSSLLNLISGQDVAITSNVPGTT
                     TDVVEKTMELLPLGPVVFLDTAGLDDKTDLAELRRKKTSQALKRADAVILVAEAGVWT
                     EYEERIVQETEMAMIPLVIAVNKIDLGSPDPNYLAIIGEKTPRLLSVAATNDKGREAC
                     LRELKRHLAASMSGEAADSPSLLGDLLPPGGLAVLVTPIDLQAPKGRLILPQVQTIRA
                     ALDIDAAALIIKERELSHLLGQLKESPRLVVCDSQAVLKVTAGVPREIPCTTFSILFA
                     RQKGDLNVAAAGAAAINSLRPGDQVLIAEACSHHPLADDIGRVKIPRWLRQYVGGEIQ
                     TEITAGKDYPRDLRPYRLIVHCGACMFTRREMLRRLKEAREQQVPITNYGLAISVTQG
                     VIERTLQPFPAALAAYERELAAPASNINGMRAQ"
     gene            24987..>26119
                     /gene="hydG"
                     /locus_tag="PLT63_03435"
     CDS             24987..>26119
                     /gene="hydG"
                     /locus_tag="PLT63_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HOD97807.1"
                     /translation="MKDFIDDELIEQLLEATRRPTAARVREVIGKARELKGLSPQETA
                     ILLQAEDRDLVSEILQAAREIKRMIYGNRLVLFAPLYIANYCANNCLYCGFRRGNKEL
                     NRVALTMGQIRGEVEVLAREGHKRLLMLCGEHPDRSGLDYFLEAIETAYGVKTEKGGE
                     IRRINVEIAPQEVEGYRRLKKAGIGTYVLFQETYHHETYRKMHRDGPKADYLWRLTAM
                     HRAQEGGINDVGIGALFGLYDYKFEVLGLLMHARQLERDCGVGPHTISIPRLEPALNA
                     PAAMTPPCPVSDEDFQQLVAVIRLAVPYTGMILSTRETPAMRREIFDHGVSQISAGSR
                     TNPGGYREGTSDGFRAAQFNLGDTRTLDEVIHDISTHGHIPSF"
     assembly_gap    26120..26219
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <26220..26536
                     /locus_tag="PLT63_03440"
     CDS             <26220..26536
                     /locus_tag="PLT63_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416985.1"
                     /note="in Escherichia coli this enzyme functions in
                     thiamine biosynthesis along with thiFSGI and IscS; with
                     ThiFSG catalyzes the formation of thiazole phosphate from
                     tyrosine, cysteine and 1-deoxy-D-xylulose-5-phosphate;
                     forms a complex with ThiG; contains an iron-sulfur center;
                     in Thermotoga this enzyme has an extra C-terminal domain;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HOD97808.1"
                     /translation="LDEVIHDISTHGHIPSFCTACYRLGRTGQDFMDLAKPGLIQRFC
                     RSNAIFTFMEYLEDYAAEETKTAGKKLIARLVAETDPAGVRETLTGRLARIEHGERDL
                     YI"
     gene            26511..27599
                     /gene="hydE"
                     /locus_tag="PLT63_03445"
     CDS             26511..27599
                     /gene="hydE"
                     /locus_tag="PLT63_03445"
                     /inference="COORDINATES: protein motif:HMM:TIGR03956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HOD97809.1"
                     /translation="MESATSTSDGAGMETGAAIADAVRNGELTREGIVRLLEIADPEE
                     LQKLFIAADAIRQRFVGSEVFLRGIVEFSNFCRRDCLYCGLRLSNRDLPRYRMEEGEI
                     LAAAHRIKAAGISTIVLQSGEDPCQSTVRICRLIRRIKDETSLVVTLSVGERPLGDYL
                     AFREAGANRYLLKQETSSPELYARFRPGCRHEERQQCLRHLKELGYEIGTGNIVGLPG
                     QNTADLAADLLFIREIDADMVGIGPFIAHPQTPLAPCPNGSPAMTLKVLAVARLLTRS
                     TNIPATTALATIAPQGWRDALQSGANVIMPNFTPVVYRGLYEIYPGKSSASTNVPDFP
                     EMLEQELRDSGRIIGTGSGFRKRQEISG"
     gene            complement(27619..28065)
                     /locus_tag="PLT63_03450"
     CDS             complement(27619..28065)
                     /locus_tag="PLT63_03450"
                     /inference="COORDINATES: protein motif:HMM:NF024138.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOD97810.1"
                     /translation="MIVQKGNPKGIKRIMDLARNDISFVNRQAGSGTRILLDYNLVRS
                     GLSPEAVKGYGHEEFTHMSVAVDVLSGAADCGMAIYAAARALDLDFIPLDREQYDLVI
                     PTAYLQDPNIQAVLETIRSRKFRDRAAALGGYDPSRSGELVMELEP"
BASE COUNT         5932 a         8033 c         8299 g         5713 t
ORIGIN      
        1 gcgatcgccg ccttccatga cgaccggctg tacgtggaaa aatatatcga aaaaccgcgc
       61 cacatagagg tccagatcct ggctgaccag tacggacatg ttatccactt gggaacccgg
      121 gattgttcca tccaacgacg gcaccagaaa ttgctggaga tcgccccggc ggatctgccc
      181 gcgccggttc tcgaagccgt ccacgcggcg gcggtgcggg cggcccgggc ggctggttac
      241 gtgagcgccg gcaccgtgga gttcctcgtc gattcccgga ccaatgaatt ctggttcatg
      301 gaggtgaata cccgcctcca ggtggagcat acggtcaccg agatgatcac cggcgaggat
      361 ctggtgcagg aacagatccg gatcgccgaa gggagaccac tgcagatcct tcaggaggac
      421 gtgcgcctac acggcatagc cgtggaggtg cgcatcaacg ccgaggatcc gaaaaacaac
      481 tttcttcccg aaggcgggaa acttatcgag atctatcatc cgcccggagg accgggcatt
      541 cgcattgacg gcaacgctta caagggctac ctcgtcccca ccgtttacga ttccctcctg
      601 gccaaactca tcgtctgggg ctacgaatgg gaacaaacgg taagtcgcct ccagcgggcc
      661 ctgaaggatt tcaacatcgt ctggccgaaa accacgattc ccctgtatat ggccatctgc
      721 gacgaagacg atttccgcca ccgccgcttc cataccggtt acctggatga gcaccccgag
      781 ttgttcaaat atcccgatat ggaacatttc gttttagacg atgcacttta caaactgatg
      841 ctggcccccc atgaaggaat ggaaggacgg tcgggggaag agcgcaagaa agaccgccag
      901 cccggctggt ggatgaacaa acgcatcctg accgcccagg agattgaaga actcaagaaa
      961 gaggggattg tcaagtcttc catcaaggga aaggtgacgg atatccctgt cgaggtaggc
     1021 gacgaggtga tggtgggaga cgtcctggtg gatctggagg cgatgaagat gcaccaccac
     1081 gtcatctgtg aagtggacgg gaaagtggcc gacatccttg tcgaacccgg cgatgccgtg
     1141 gatgtcggcg acaccctggt catcgtgaat gtccgcggtt agcgcggccc gaatcatcgg
     1201 gacgacgact gccgaaaaca taaaaaaccc cgccggcggt gcaaaccggc gggggttcca
     1261 attattgtcc gcggcttttc cgtttgtgtt caaagaactt caccgtatgc gaggcaattt
     1321 gcggccatag cttgcggata tttacccaaa tacaagtttt cccttcatct catccgaacc
     1381 ggcatcagaa gtgaggacgc cgggggcggg gccgggactt tccgccgccg gggcggcggg
     1441 tgcttcgggg acggactttc ttttccggat atagggcttc cggtttgacc agcatctcct
     1501 cgtccggctc aatgcaagga agcctccggc ccatgaactc ctcgatgggg gcgatgtaaa
     1561 aggcgtctcc ctcgtcggcg aagctcaccg ccgtcccgct ctgtcccgcc cggccggtgc
     1621 gcccgatacg gtggacatag tcttccggat cgtggggcag ggtgaaattt atgacatgat
     1681 ccatgctctc gatatggatg cctcggccag ccacgtccgt ggccaccagg accctgatct
     1741 tgccgctctt gaaatcttcg agacggcgga tccggctttt ctgcgggatg tcgccggaaa
     1801 gttcggaaca gttgacaccg tagcgggtca gacgttcggc gaggcgcctt acctcatcgc
     1861 ggcggttgca gaagaccagg actctttcca gatcctgccg gacgattata ttatgcagga
     1921 gatcgaattt ctgctcctcc gtaacgatat agacgatctg ctccactgta tccacggcca
     1981 cctgctccgg ctctatctcc accatgacgg gatccacagt ccaggaggag gcaaggcgga
     2041 tgatcgtccc cgtgagggtg gcgctgaaca tgagggtctg gcgcttttcc ttgggagggg
     2101 tgctatggat gatcatccgg acgtcgggga tgaaccccat atcgagcatt cggtccgctt
     2161 cgtcgatgac gaggacctcg atcttcttga gcaccacgtc accgcggcgc tggaagtcca
     2221 agagccggcc cggcgtggcc acgatgatat cggccggccc ggcggcaagc tggtggcgct
     2281 gcttttcata atccatgccg ccgaaaacag aaacgattct tattccgcaa tacttggcaa
     2341 gctggcgggc ctcggcggag atctggaggg ccaactcccg ggtcggagca agaatcaggg
     2401 cccggggcgt gccgaacgga cgctcttccg tgtggggatg gttgatcaac cgggtaatga
     2461 tggcgatgag aaaggcggcg gtcttgccgg tgccggtttg cgcccggccg ctggcatcct
     2521 tgccggagag ggcgctgggg aggatttccg cttggatggg ggtgcagtac tggaacccaa
     2581 gatcgctgat ggtgtggaga agcggtgccg gcagatcgaa atcttgaaac ctggttttgc
     2641 cgggagcggc aggaaccttg aactgtctgg gatcccagga atcgtcggta accggtgcgg
     2701 gttcgggaac agcgccggcg tcgatagctt cgggccgttt tttccccttc ctagagggac
     2761 ggcggcgacg ggtgctttgt cgcctttccg aagtcagcga atccgctccg ccgccggcat
     2821 cgcttccctc cgcctcgggt accggtgacg aaggtatggt ttttgtaagg ttttctgtgt
     2881 cctccatagg gctctcttct ctctgtcggc ggtctcttag tgccgcggaa cgtttatgag
     2941 ctaattttgt gcgaatcata atctcatccg ataccggatg gcaaggatta ttatgcccgg
     3001 ggattggaga gaccggggcg acgcccttga ccggatggcg gaactgggct ataaataaat
     3061 caggtgcgac attggggagc taggagtgat cgcggtcgta atcagaatga tttcaaggag
     3121 gcaatacctg agatgaagat agagatatgc gctgcgtctg gttgtgcccg ggcgataatc
     3181 atgatcacct ttgtcgtggg atgcttgttg tccggctgtg ccgccgggcc gccacgcctc
     3241 cagccctcgg gactcaatac cgtcttcgcc ggaaaagacg gcatgccttt cgctgactat
     3301 atcaaacaga cccgggcaat gatgctccga gcccgggtgg atattaatga cggcaaccgg
     3361 gaaatggtcc tcaacgccaa cctgcccttt gaactcgcgc cggacaccaa ccttttcccg
     3421 ctagggaagg acgggaaaca ccgccggggc gcccttctcg tccacggact ctccgactcg
     3481 ccgtatatgc tgcaacccct ggcccgccac ctccggaacc gcggttttct cgtccggacg
     3541 atcctcctcc ccgggcatgg gacggctccc ggagacctgc tggaggtgca ctggcaggaa
     3601 tggagacggg ccgtgcaata tgggatcgac ggcatgaagg gagaggttga ggaactctac
     3661 ctaggcggct tttccaccgg cgccgccctg ggcgtgctgt ccgtcttgag cggtgaagac
     3721 ataaagggat tgattctcat ttctccggcc ctgggggtga aagaccggcg tgtggcgttg
     3781 gccggggcgc tccggagtgt gagggactgg gtcggcgaca tccgggacga tgtggattac
     3841 gcgaagtatg aaacctttgc ggtgaatgcc gcctatcaga tctaccttct taccggggag
     3901 atagacaggc ttctcgctgc gggtaaacgt attgaagtcc ccgtattcgc tgccttatcc
     3961 gcggaagatg tcaccgtcga cgccgggcgg gcccaagagg tttttcggcg ttacacaacc
     4021 tcggaacata acgtcgtgat cctgtatgcg cgagatccgc ggccggagat atgtgccgga
     4081 tcaggaacga tttattgcga ggggagttat ctgccggagc tgcggatact ggatttctcc
     4141 catgtcgccc tgccgatgcc ttgcgatgat ccccattacg gtctgcgggg aggatacaaa
     4201 agctgcctcc attacggagc cgatcgagaa aaaagaggga tttgccggaa tgacgacacg
     4261 atttggcagg gagagatatc cgacgccaat ctccagtcct tcaccctgcg tcgtctgacc
     4321 tgtaatccca aattccctgg tatgcgggag cggctcgatc aattcatcag aaacgttgcc
     4381 cgataaacag acccagggtt tggtgtcctc cctcggcgaa cccgtaagca atataggccg
     4441 gcccgatgaa ggtgtccgcc ccgatgaagg cgctgccggc gtaaataaga gaacccagag
     4501 tgatgtcgct ccgtttctgc caggccttgc ctgcttccac ggagaagccc agataaaggg
     4561 tggtgcgaaa atcgccgagg ccggcggccc ccatattccg gaagcagacg atccgcgcca
     4621 ggcccacctc ctgacccgac agctcatcgg tcaggtaccc ggagagattc aagaacccgc
     4681 caatactgta cgaatcctga atcttgctgt cgccgtccag ggtgccaccg tagatgattc
     4741 cagggatgaa ggtcaggcgt ccccaggtct tggcgccctg gatgttggca ttgatcccgg
     4801 cgaccttgta gtcggaaccc atttccggga gattgaactg gctggtcacg ttggcgctta
     4861 agccccgctg gggaaaattg aagttgtcca tggtgtcgta ggtcaaagat gaaaagaaac
     4921 cgccgatatt gaaactgtcc gtgggcaggt cggcggcccc gacattgacc cggtaggcgc
     4981 cgtagccgcg gcggatgccc aggcgcaact ccccccagtt gccaaactgc cgaccaagat
     5041 ccagaccccc tgaaaattcc ttgagccgat atttagccat gatatccgct gtatattccg
     5101 ggctgtagat gtaggcattc cattcccggt attcgagccg gggggcgaaa aaataatgcg
     5161 tggagtaatc aatgggctgg taaaattccg aaaacaagcg tgtcgtttcg ccgatctgca
     5221 attcattccg ccattcggcg cccaaggtgt tgatctcggt ctgggtaatc cgcgtgccga
     5281 cggtatagga actggagcct ttgaaattgt ccgccagact gatgttgaag cgcatgtaag
     5341 tgggacccca cgatttttca atgggattga aaaccaggcc ggttttgttc cttctccttt
     5401 cgagatgaaa atcgacccgt tcaaaggtgt ccagaccata aaggcgggtg atatcctttt
     5461 cgagggtttc tatgttcagt ttttcccccg ccttggtttc gatctgggcc tcgagtaccc
     5521 ggggagaaat cttggtacgg ttttccgccc tcgtcttgac acccggcaag tgtgccagtt
     5581 tgcttcccag gtcgatctcc tttccttcgc tgccggtttt aaccgacgga aggggagatg
     5641 cctgggcggg ggacgctgat ttgaattcca ccttcacgta atcaatgacc ggcggctcgg
     5701 agggctgccg ccgctgatgg gccagataaa cctggaattt ttccggggca atggaaagac
     5761 cggcaaggcg ttccttcatt ttgcccaccg ccgccgcgcc gatcttcata gccgtttcgg
     5821 ttttagaaaa atccgtggtt cccagcgggc cgagatcggg ctggatgagg atgtcatccg
     5881 gcttcagggt ccttagttgg gcctggacat ttctctggat gaggatggtc atgacctggg
     5941 cggtgatact ggcagtggaa ttgagcccct ccctggtcct gaggggggtg ccgatatcga
     6001 cgacgatgat gatgtcggcc cccatggcgc gagccacgct taccggaagg ttgttggcga
     6061 ttccgccgtc cacgagcatc cgtccgttga tctccatggg tacgaagatc cccggaatgg
     6121 acatgctcgc ccggagtgcc tccgccagat ctcccttacc cagaacaacg gtgtcaccgg
     6181 tttcgatgtc cgccgccaca gcccgaaagg gaatcttgag gtgatcgaaa tccttgatgt
     6241 ccgcggtgtg cagcaggagt ttcttcagga ggatattcag attctgtccc tggaccaggc
     6301 ctcgggaggt ggccaggcgg ccgtttcgga ccccgaggtt aaagtcgatt ttataatcca
     6361 tggagtcctg cttgcggcgg aaggagagtt tatccaccgt ctgcttatcc gtgaaggcct
     6421 catgccaagg gatgccggtt acaagttttt ccaactcctc cggggaggaa cccgccgcgt
     6481 agagtccgcc gactatggcc cccatgctgg tccccgcgat acaatggatg ggcacccgca
     6541 tttcctccag gaccttcagc accccgatat gggcaatccc ccgggcaccg ccgccgctca
     6601 ataccagacc gatccgaagc ggccgcggct cttcggcgcc ggtcatggaa ggtgcaatga
     6661 tgtttaaaca aaggaaaaga aagatgaaga tcgcccggaa gggcaaagaa aacggccggc
     6721 gttttcgcgg aggcatcata ggggtcggaa tattccttct tttctctttc ccggccacga
     6781 ttagctgacc tgtgacgttt tgtcaatccg gacgtttacg ccgcggaagc aggatcagaa
     6841 ctggcgtcct tcccgccccc caccgccact ccgccggggg ccgccgccgc gtcgaggcgc
     6901 ttggctgctg aaaccgccgt gcctgctgcc cgttccccgg ggccgttcgt cctgatctcc
     6961 cttcggtttg gcctcatcga cccggattgt cctgccgagc agttcgccgc cgttgaatgt
     7021 ttggatggct tcctgggcct gcgcttccgt tgccatctcc acgaatccaa atccccggct
     7081 cagaccggtg atcttgtccc gaattatcgc cagagaaacc accttgccga tctcactgaa
     7141 attggccatt aaatcatctt cagtaacacc ctccgccagg ttgccgatgt aaagcttttt
     7201 taccatgctt tcttcctcct tgcttcccct tttgacccgt cgccatgccc ggcagagccg
     7261 ccatccatcg gcattgatgg catataaaaa accaccggac tctgcggagc acggtggcct
     7321 tccttgttcc acaccttgca gacaggatgc tctgatcttc tcttgaccca aatcagcagg
     7381 aaaataatat agatggttct ctctaatctc cttgaccggg aatgtcaaac gatattttgc
     7441 ggaacggaca aaagctgcta ttcttacttc aacggtaaat tatcttgttg acatcatcaa
     7501 tatgttgtgg caagtagggc acctcatact agatattgta tttaaaaaat cattttcatt
     7561 atttatgaag cgaggagaca aatgaaatga aaaagaagac ttccccgtcc gccatcccct
     7621 gcctgaccaa aaatgccatt accgtcctgg aaagacgcta cctaaggagg cacacggacg
     7681 gccgcgtcct cgaaacgccg gcccacatgt tcaggagagt ggcggagagc attgccgcgg
     7741 cggaacggat attcagcccc caggctgata cagggaagct ggtggagaag ttctatcgca
     7801 tcatggccga cctggaattc cttcccaatt ctcccaccct gatgaatgcc ggccgggagc
     7861 tggggcagtt gtcggcctgt ttcgtcctcc cggtggggga ctccatggaa gagatcttcg
     7921 atgccgtaaa atataccgcc ctgatccaca agtccggcgg cggcaccggt ttttcctttt
     7981 cccgcctccg gcccgccaac gatgtggtga tgaccaccac gggtatctcc agcggtccca
     8041 tttccttcat gcgggtcttc gacatcgcca cggaaaccat aaagcagggg ggcacccgcc
     8101 gcggggccaa catgggcatc ctgcgcgtgg atcatcccga tatcatggat ttcatcatgt
     8161 gcaaggccga caagaaccag ttgaacaact tcaacatctc cgtcggtctg acggaagtat
     8221 tcatggccgc ggtggaggcg gacgagaact acgacctgat caatccccgt aacggccagg
     8281 tcaccgggac actgaacgcc cggaaggtat tcaaccgcat cgtcgtccag gcctgggaga
     8341 acggggagcc ggggatcgtc tttctagacc gtctcaaccg cgacaatccc acgcccgacg
     8401 tcggggccat cgaatcgacg aacccctgcg gcgaacagcc gctcctgccc tatgaatcct
     8461 gcaacctggg atcgatcaac ttaggcaaga tggtgacggg cggcgccgtg gactggaaac
     8521 gcctccggga ggtggtccat ctggccgtcc attttctcga caacgtcgtg gaggtcaaca
     8581 gataccccct gcccgtcatt gccgagatga cctttggcaa ccgtaagatc ggcctgggcg
     8641 taatgggctg ggcggacatg ttgatcatgc tggggatccc ctacgattcc gaggaggcgg
     8701 taacgctggc cgggcaggtg atgaagttca tcaaggagga gggtcacaac gcctcccggc
     8761 atctggcgaa acagcggggc cccttcccga atttcagcgc ttccctctat gccaaaaaag
     8821 gcgagccccc cctccgcaac gctacgatca ccaccatcgc cccgacgggg acgatttcca
     8881 tcatcgccaa cgcctcttcg ggggtggaac cgatcttcgc cgtgtcttac atccgccagg
     8941 tcatggacga cgacatcctc gtggaggtcc atcccctgtt tgaaaagacg gcccgacagc
     9001 gggggtttta cactccggag ctaatgaaga ggatcgccga gcaggggacg ctccacgacc
     9061 tggaggagct gcctgcggac atccgccgcc tgttcgtcac cgcccacgac atctcgccgg
     9121 aggtccatat cgggatgcag gccgccttcc agaagtacac cgacaatgca gtgagcaaga
     9181 cggtcaactt cccccgggac gcctcggtgg aggaggtggc caaggtgtac cgcctcgcct
     9241 atcaactgga ttgcaagggg gttaccatct accgcgacgg ctcccgggac aagcaggtcc
     9301 tgtctcgagg caagggggaa gaaaagacgg cccgcatcgg cggcgacgaa gaacgcaagg
     9361 tagtgaaaag ggaacggccc aaggtcttga agggctggac ctatcagatg cagaccggtt
     9421 gcggccccct ctatgtaacg gtcaatcagg acagtaccgg cctttttgaa ctcttcacca
     9481 ccatgggaaa ggccgggggg tgtgccgcct cccagaacga ggccatcggc cgcatggtgt
     9541 ctctggcctg gcggagcggc attcaggccc gacaggtcat caagcagctc cagggcatct
     9601 cctgtcactc cccttccggc ttcggggaaa acaagatcct gtcctgcgcc gatgccgtgg
     9661 ccaaggcgat ccagtcccat atggcggcca acggttacga aacggtgcgg gagaaggtgg
     9721 ccctggtcaa gggggcctgt ccggaatgcg gcgggatcgt ctctcacgag gggggctgtg
     9781 ccgtctgccg agtctgcggc tacagcgaat gcgcctgaga cgtggccgga aagaatttgt
     9841 caaggcgatt atgtgctcga atgtccttca gtgggtgagg ccggccaagc ggaaggcctg
     9901 ggcggtcaac tccgccggga tcaactctcg ccgcatctgt tccgtaagtt ccgccactcc
     9961 cagatactgg gctgaggcgg cgatgaccgg ttcgcatcgt ccggcattgt ccagagacac
    10021 ggcgccgccc gaggtccaga agaaaatcct ggcaccggcg ttgccccgaa ccctttcact
    10081 tcggccgatg aagaccagcc gttcccccgg tcggatgtcc cggtagaagg tgtagccgat
    10141 tcgcaccgtt accgccccgc tggccgccat catgaaaccc gcccagccca tggtttcatc
    10201 caaaagtgcc agcgagggca ggagcgagag gcggccgcca ctctgaaacc gataaaatgt
    10261 gttttcatcc gtgacatcga aaccagccgc ggcataggcc agacggggcg tgccgccgtc
    10321 gccgcctccg agaaggaaac gtcgctccag gccaggttgg cgacgggcga cgccacagac
    10381 gaaacaattg cggtagtagg gcaggggaat ccccccctcc gccggattcg agatcgtggc
    10441 ggggagataa gactggaaag tttcgcgccg ctgcgggtcg tctatgccgt agccgatgac
    10501 ggccgaaagg tacgggtcgt tattccccgg cgtgaagatg gttccccgga tcccttccgg
    10561 cgcggccagg accgtgaact ccactttatc accgatacgg actccggcgc cgcccaagcg
    10621 gaaatccact gtaaggggat attcaggacc tcggtctttt tccgtccatt cttcccggag
    10681 cagcgcggcc agttccatga cggcccccat ggcgataccg ccgtggggta tcctccgcca
    10741 gccttccagg cgttcgtcca tggtcatggc ggtatgcacg ccgtcgtggt cccggactat
    10801 cccggcatcg gcgaacagaa attccctcgt aaaattcgcc cggttctttc caatggggac
    10861 ggaaggctcc cggcttgacg gtgaagacat aactacccct gacaattcat gattaggcgt
    10921 cggactccgg aagtgattcc ggaagcggga agacgaaact ccgcttccga ccatgccggc
    10981 tcccggaggt catcacaccc gatgggggaa agcagtttca cgccgccgcc gctgatcatt
    11041 aatactttcc atacctaatg tcaatcccac ccttttccat tatgttaata taaatataat
    11101 cagcaattat cttgacttag cgcggccaat tattgtatcg agattctgtc ataactgaca
    11161 taaaaagact ttttacccaa ttaggaggcc tgtaatgaag gatgcagtat tttccctgaa
    11221 cgggaaagaa accgtttacc agcccggtca gtcggttctg gaagcggcaa gggcagccgg
    11281 ggtgaacatt cccaccctct gttacctcaa ggatacgacc cccaccggcg cctgccggat
    11341 ctgcctggtg gagatcgccg gcgcccggac tttgatggcc gcctgtacca ccccggtggc
    11401 ggcgggcatg gaggtgttta cggaaacgga acgggtccac aagacccgga aactaaatgt
    11461 ggaactgctt ttgtccagcg gccgccatga ttgcctggtc tgcgagaaga acggggattg
    11521 ccgccttcag gatctggcgt attattacgg aatcaaggag atgcgctttc ctcaggatca
    11581 ggatttctac cccactgaag aggaaaaccc attcatagta cgcgatttca ctcgttgtat
    11641 ccgctgcggg cgctgcattc aggcctgcaa cgaacttgtg gtcaaccggg ccatcagcta
    11701 cggttatcgc ggcaaggaca gcaaggtcgt caccggcggc gacggcccgt acctgggcag
    11761 cgagtgcgtc ttctgcggcc agtgcgtgga ggtctgcccc gtcggggccc ttacggaaaa
    11821 gaaggcccgg ggcaaggcgc gggcctggga gacaaagaag atccgcacca cctgcgccta
    11881 ttgcggtgtc ggctgccagc agtggctcca cgtaaaggac ggccggatca ccaaggttac
    11941 ggcggtggag gatggagagc cgaatatggg gcggctgtgc gtcaagggcc gtttcggtta
    12001 tgacttcatc tattcggagg accgcctcaa gacgcctctc atccgtgaaa acgggaactt
    12061 ccgggaggct tcctgggacg aggccctgga tctggtggcg ggaaaattca aggaaatcat
    12121 agccaaggat ggtcccgacg ccattgccgg cgtcagttgc gcccgaagta tcaacgaaga
    12181 ttcctatcaa atgcagaaac tgtttcgcgc ggtgctcggt acgaacaaca tcgatcactg
    12241 cgcccgtacc tgacacgccc cgacggtcgc cggtctggcg acctctttcg gttccggggc
    12301 gatgacgaat tccttcagcg atttcgccaa ggccaagatg atcctggtca tcggttccaa
    12361 catgacggag gcccatccgg tggcggcgtc actgctgaag atggcggtga tcggcggcgc
    12421 cgagatgatc gtggtggatc cccggcgaac gcccctcgtg gatctggcca acctgcatgt
    12481 acagataaag gtaggctccg acgtggcctt cctgaacggg gtgatgaacg tccttatcac
    12541 cgaggatctc tacgataaga attttgttga atcctgcacc atgggtttcg aagaactgaa
    12601 gcagaaggtg ctggaatatc ctccggagcg ggccgcggcg atcagcgggg tcggggccga
    12661 cgtgatccgg accgtggcgc gccgtctcgc ggccaaccgt cccgccatgc tcatgtacac
    12721 cctggggatc accgaacata tctgtggcgt caacaacgtc atgtcctgcg cgaacctcca
    12781 gatgctcctg ggcaatgtcg gcttcgaatg cgggggcgtc aaccccctgc gggggcagaa
    12841 caacgtccag ggggcctgcg acatgggagc cctgcccaat gtcttcccgg gctaccagcg
    12901 ggtggacaac ccccaggcgc gggaaaaatt tgccgccgcc tggaaggtgg aaaaacttcc
    12961 ggaaaaaccg ggactgatga ttccccagat gatcgacggc ctcctggacg gcaaggtgaa
    13021 ggccttctac gtcttcgggg aaaatctcgc caacacggag ccggacatcc ggcacgtgga
    13081 gaactgcctc gccaccgcgg agttcctggt ttgccaggac atcttcccca cagagacgac
    13141 tcggttcgcc catgtggtgc tgccggcggc ggcatggagt gagaacgacg ggacgttcac
    13201 cagcagcgaa cgtcgggtca accgggtccg caccgccagt gttccccccg gggaggccaa
    13261 acccaactgg tggatcttca aggaaattgc ccggcggatg gggtatgtct gggaatcaga
    13321 cagcgctcag gagctttggg acaacgaggt ctctcaactg gcgccctcca tggcggggat
    13381 caagtatttc cgcctggaag gcaacggtct ccaatggccg gccccgagct tggaatccga
    13441 ggggacatca tatctccata aggacggctg ctttacctgc ggtttggggg ttttcaaggc
    13501 gatcgactgg ataccgccgg cagagatccc cgacggggaa tatcccctgg tgctgagtac
    13561 gggtcgccgc ctctaccact accacacccg gacacaaacc gggcgttccg acgggctcaa
    13621 cgtactgctc agcgaggaaa ccgccgatat ctccgaggcg gacgcggata aatacgggat
    13681 tgcggacgga gaaatgatcg cggtgaagtc ccggcgtggc cgggtggccg tgaaggccaa
    13741 gataaccccc caggtgccgc cggggatggt ctggatggcc ttccatttcc gggagggatg
    13801 cgccaactgg ctcacgaatc ccgtttacga tcccattacc cagacggcgg agtacaaggc
    13861 ctgcgccgtg gcggtggaaa agatcacctg acgggcgacg gccggggaag tttcggggga
    13921 agtgaagcgc cgcttgggcg ggcttcactt ccccttttta ttttcctgat ccggcctctt
    13981 ctctccgtcc agaagggtgg tgagttcgcg gctggtcttt ttcagatagg aatcggcgtc
    14041 tttttccagg cgccggaatt tttccaggag cgtcatggcc gcgggggtta gggtcatccc
    14101 ttttttcgcc gcgtcgcttt cgatgagttt gatgcccaga cgatcctccg atgcccggag
    14161 gcggccccag gcggcccgat aggacatgtt gagctttttc gccgcggcgt tgaggctgtg
    14221 acattcggcg acggcctgga ggagttcctc gcggccgtgc ccgaagatca ccttgccgtc
    14281 cttttccagc cagatcttgt atttgatctc catccgcgct tacctcctcc ccctacggag
    14341 aaaatgtaat gaaaggcccg tgaatagtca agttttttga cctggcgagc tcagcttaca
    14401 tagggagatt tcccctggca ggccaggcag gaggcgccgt cgcggagggg ataggcctcg
    14461 ccgcattgcg ggcacacggc ggtggacccc aggcgcctct tgacggttat ttccggcttt
    14521 acccggactt cctgcatgct gaggaccgag gcgccggcct ccttgatctg attcagaagc
    14581 gtttccaaat cctgttcccg tttcggtttc agcttgaaga accaggcatg gaattccggc
    14641 caggacttca gcttctccgt gtcgagatag acccgaatgc ctttacccga ctccttttcg
    14701 taaagtgtca cggcaaagcg ccccaggtcg aagaccttca gccagccgtt gccgatggtg
    14761 cagggggtga ggagttgtac ggcgtcgggg aggcagaccg aggtctcaca gacggcgtcg
    14821 aagaattcac cctccgggag atttttcaag gccgtctcga cgatgaagcc gccgatgatc
    14881 aaccccgggg ccagattgcc gtgaaacgat tttacgagat ggatatattc atcatatgaa
    14941 taagagccga atttcattaa ctattgtact cctttccaat tggtgtcgcc gcaaccttcc
    15001 caacctgttc ccacccttcc cgacgtaagc tccggcgggg gatggatggt gacggcgaag
    15061 ggtcggtagg caagggacag gcgcgggtcc cgggccaggg cctggcagat ggtgcaccgc
    15121 tggcgctcat cgccggaaat ggcattcggt ttgcgccgtt gcagatgatc gattccgaag
    15181 cgtgcccagt aatcgtttca ataacttata cctcctttaa catatgtttc atttggcctg
    15241 atacagaaat gcagcaaaaa agtcaaccca agatgtgtca tggatagcat atatcccttg
    15301 acaccgccgg ggtcgattgt atagatgggg gcgctgtgcg gaggagccct tacgggcgca
    15361 ggcatttacc ggggttggat aaaaggagcc aaaaagatga gagatgcgga aaaaacggag
    15421 gagaatctgc gggagatcat ggcgcgggaa atcgccggaa acccccacgc cgaggcaatt
    15481 atcggcgcct ttcagcctct gcttctggcg aggaggagat tgctggcggc tgaaaaatgg
    15541 ccggccgtga ccttgacggg attcgatccc gcccgtttcg gcgctggcat tccgttgctc
    15601 cgccaggtgc aatttttttc cgccgccgat ccctgggggg agatgacaga catgatggcg
    15661 gccgccatcg gggcgggatt cccggtcctc aaccgcgaca tggagcgtat cgccggggct
    15721 gcccgcaaca aggacataga tttgttttca tacttccata agctggtcga tgccggcagg
    15781 gacgagaacc tcgtattttc ctgggcggag aaattgtcgg tttcggcgcc ggttctcgag
    15841 ttcctgctcc ggcagttgtc gcgggtcgtc ctggaaaagc gccttacgga agccgcggcg
    15901 ctgatcggcg acatagaatg gcggcacggc tattgcccgg tctgcggtga attccccagc
    15961 atcgccctca tcgaggagaa gatcacccgc cgctggctcc actgctccgg gtgcggccac
    16021 gagtggatct tcagccgggt gatctgtccc tactgcgaac acgaagccca gcagggcatg
    16081 gacttcttct acgtggaaga caggccccgg gagaccgcct tcagttgtga tcagtgccgg
    16141 cggtatctgg tgaccctcaa gggggtcagc gagctggccg atcgcgatct cgacgtcacg
    16201 gcgatgagct tgacgcacct ggacgtaatc atgcagggga aggaattctc tcccatggtg
    16261 aataccccat ggaatgcgtt acggtgagat gtctggacat atccggtctt tgccgctgtt
    16321 cttcgcctga tacatctgtt ggtccacccg ctgaacgaag tccttcatat cctcctgggg
    16381 ccgtcaaatc ggcccaaccc gccggctccg gaacgccgtg acaaccgcgg ccccttcccc
    16441 atgactttcc cggccggaag gacgccggct tcttttcacc gcctaccata acactagatg
    16501 gccggaaata gtcaaagaat cgttggcttt gccgcctttt cggccgagcc cgacccttgc
    16561 cgacatcgcc ccccccgaca gccctccacg caattatgtt atatttacca taaggagtta
    16621 atttgtttga gttatgttta cttttcgctt gacgctcggc ttaaaatggc gtagatatgc
    16681 cgcgcttgac atagatcaat tttgacataa gatttattcg ctttaattcc aaggaacaag
    16741 gaggcttcta tggagattac cagaaggggt ttcttgaagg tgtccggggc cgtgggatcg
    16801 gggatcgccc tttccggcct gggcatcgac ttgcgccccg tacacgctta tgcggacgag
    16861 ctcgacaaga tgaaccgcgt aaagatggcg aaacaggtga gcacaatttg cgcttactgt
    16921 tcctgcggct gcggtctcat ctgcagcgtg gacaagctga ccgggaaaat attcaacatc
    16981 gaaggcgacg ccgaccaccc catcaacgag ggaacgcttt gtgccaaggg ggccggtttc
    17041 ttcgatctga cggaggccaa caagcaccgg ctgaagaagg ttctttaccg cgccccgtac
    17101 gcaacggaat gggaagagaa agactgggac tttgctattc ccaggattgc ccgactcatc
    17161 aaagacacgc gggacaagga atttatcacg aaaaacgcca agggtgagct ggtcaaccgt
    17221 tgtgaaacga tcggccacta cggcagttcc aatgtcgata atgaggaatg ctggctcatg
    17281 tccgttaagg ccagggccct cggcatggtc tatatcgatc atcaggcccg ggtctgacac
    17341 agcccatctg ttgcggctct gggagagtcg ttcggacgcg gttccatgac caaccattac
    17401 gtagatatgc aaaacagcga ctgcatcctc attatgggga gcaacgccgc ggagcaccac
    17461 cccatggcgt tcaggtggat gctgaaggca aaagagcgag gcgcaaaact tatccatgtc
    17521 gatccgcgct atacccggac ctcggcgcgg tgcgattacc acgtccccct gcgttccggg
    17581 acggacatcg cctttttggg cgggatgatc cattacatct tggaaaacga caagtggttc
    17641 aaggagtacg tcctccagta caccaacggc tccttcatcg tggggcccaa tttcaagttc
    17701 gaggacggtc tcttttccgg gtatgacgcc gaaaagagga aatacagcaa ggacacctgg
    17761 gtcctggaaa aagaaggcag cggcatcccg aaacgggaca tgtccctctc cgatccccgt
    17821 tgcgtcttcc agcagttgaa gacccactat tcccgttaca ccctggacaa ggtttcgagc
    17881 attaccggtg tctccaagga aaacctcctg aaggtttacg cggagttcag cgccaccggt
    17941 gttccggaca aggcgggcac ggaatgctac gccctgggct ggacccacca tacaaccggc
    18001 agccagatta tccggaccat ggccatcatc cagctcctac tggggaatat gggcatcgcc
    18061 ggcggcggca tcaacgccat gcgcggcgag ccgaatgtcc agggttcaac tgatcaatgc
    18121 attttgtacg gcaatctgcc cggctatctg aagatgccct cggcttcgct tgaatctcta
    18181 gaccattatc tggaaaagta cacgcccgta tcgaaagatc cccgctcggc caattactac
    18241 tcgaattatc cgaagttctt cgtcagtttc ctcatgtccc tcttcgataa caaggccacc
    18301 aaggaaaacg gtttcggcta caactggctc cccaaaatgg acgatggaaa acactattcc
    18361 acgatgcaca tgttcgatgc catgtatgcc ggtaaggtga agggatactt ctgcgtcggc
    18421 gcggatacgg ccgtcagcac cccgaattcc aaaaaagtcc gcaaggcgat ggaaaacctg
    18481 gactggctgg tgggtgaaaa catcttcaac aacgaaacct acgaattctg gcgaggcccg
    18541 ggcgtcgatc ccaaaaagat caagacggaa tgcttcctcc tgcccgccgc cgccacgatg
    18601 gagaaggaag gctcgcagag caattccgga cggatgatcc agtggaagta tcaggccgcc
    18661 ccggcccccg gcgacggtct gcccgtgggc gagattacga tcaagatcat gaaggcggtc
    18721 aaggagttgt acgccaagga aggcggaccc aatgccgaac ccattctcaa tctctactgg
    18781 gattatctgg acggcgcggg gcacttcgac cccctgaagg tcgccaaacg actcaacggc
    18841 gtcttcctga aggatacggt catcgaggac aaggccaagg gaacgaaggt cgaattcaaa
    18901 aagggccagt gcgtgccggg cttcggcaac ctccagctcg acggctcgac ggcgtgcggc
    18961 aactggattc atagcggcag ctttaccgcc gacggcaaga ataacatggc ccggcgcggg
    19021 aaggatgatc cgaccggatt ggggatcttc cccaactggg cctttgtctg gcccgtcaac
    19081 cgccgggtca tgtataaccg ggcttcctgt gatatcaacg gcaagccttg ggatcccaag
    19141 cggaaggtcc tggaatggaa ggacggcaaa tgggtggggg atgtgcctga cggcccctgg
    19201 ccgcccctgg ggttggaggg gggaaaatat cccttcatca tgcaaaaaga cggcatgggc
    19261 gccatctttg gccccggcat ggtcgaagga ccgttcccgg agcattacga accgctggag
    19321 ggaccgctgg cgaagaatcc tatgtcctcg cagctcatca atccggcgat cgaaatcttt
    19381 aagagcgatc tggacacgat cgccagagcg agcgaaaaat acccctatgt ctgcacaacc
    19441 tactcctgta cggagcattg gtgcacgggg gcgctgacgc gctggcaggc ctggcttttg
    19501 gaggccatgc cggaagccta tgccgaaata ggcgagacat tggccaagga aaaaggcatt
    19561 cgcaacggtg agcgcataaa gatatcctcc attcgcggcg acgtgaaatg cgtggcgatg
    19621 gtgacgaagc gcttcaagcc cttccaggtc gagggcaaga cgattcatca gattggcctt
    19681 cccttcaatt acggctggct ctttccgaag gatacgaaag acgacagtac gaacttttta
    19741 accccaaccg ttggcgacgc gaacaccttc tgtcccgaat acaaggcgtt catggtcaac
    19801 gtagaaaagt tgtaggaggg aagggaagat gaataataaa cgtgaagtcg taaaattgat
    19861 cgatactacg ctgtgcaccg cctgccgtgg ttgtcaggtg gcctgcaagc agtggaacga
    19921 gatgccgggg ctcccgacga agcagtgggg cagttaccag aatcccccgg acctggagtg
    19981 gaacacctgg acgctgatcc gcttccagga acacgtcaac gagaagaagg aattcaagtg
    20041 gctcttccga aaggacggct gcatgcactg caccgatgcg gcctgcgtga aggtctgccc
    20101 cagcggcgcc cttcaccata cggacttcgg gaccgtggga ctgaattcct ctctctgcat
    20161 cggctgcaag gaatgcgttt ccgcctgtcc cttcgacgtc ccccggtaca accgggagac
    20221 ggaccggatc tacaagtgcg acctctgcta cagtcgtctc cggggcggcg aatcgccggc
    20281 gtgtgtcaaa tcctgtctga cgggggcgct ggacatcggc ccgaaagata agatgctgaa
    20341 gaaggcctac gccaaggtga aacagctggg cggcgacgcc aacgtctatg gcgacaagtt
    20401 cgtggggggg acccacgtca tctacgtcct ggatgagaag ccgaaggtat atgagaagct
    20461 tcccaggaac cccagtgtgc cgtttacggt gacgatctgg aaggacattt tgaagccgtt
    20521 gagtctgctg gcggcggggg gtgtgatagg cggctcgttt gtccactaca tccttcatgg
    20581 gccgaagctg cccgatgagc ccggtcagga aaagggaggt gaatgagcga tgagaaaggg
    20641 attgatacaa gcgacggatg gatttgagcg gatcgttcac tggagtctgg ccttgtcgtg
    20701 cctggcgttg tgcgtcacgg gtttggggat gatgtttcat tccctgaact ttatcgggga
    20761 gttgctgggc ggtctgaagt cattgaagta tgtgcataat tttacgggga tcttttttgg
    20821 tgtggccctg tatttcacca ttcggatgtg gtggaaggag gcgggggtgt tttccatgcc
    20881 ggaggatctg gagtggatca agtgtgcggg tggttacctt tggcatgtgg acaatcctcc
    20941 ggaggtgggc aagtacaatc ctggtcagaa gatgtttttt ctggcggtgg cgggattcgg
    21001 cgtgattatg ttccttacgg gtctggtgat gtggttcccg ttgagttttc ctccgacgtt
    21061 tgtgcggttg atgtacgtgc tgcacgcctt gggatttgtg gtgatctttg cctttttctt
    21121 tgtccatttg tatctgggga cggtgggttc tccgggatcg ttgccggcga tgctgacggg
    21181 ctgggtgacc cgggagtggt tgaagaagca gcaccccaag tggttgaagg agatggagca
    21241 cgaagggaag ctggtggtct atggcgagga gaagccttcc ggcggccacg gccactgaga
    21301 atcgagattc ccccggtggg acggcggtga ttggcgactg agcccgcatc gccgccggga
    21361 gaatataaaa atgtcacgac aaggaaagag atctgctcac catgggcagg tctctttttt
    21421 tccccgattt cctctccctt cagtacaacc atcccagcaa atcggacggc gaggcggaaa
    21481 atcttaagaa aacggggagc tgtgtcgtat aatcattaac aataacaata gtatatctta
    21541 tgccggcgca ccggcgaagt ttttgatccg ccccggaaat ggttgtaaaa aacaggatca
    21601 gccgtccccg ctctgcatct ttctcatcgg ccgggacaga cgataaaaac gatcatctga
    21661 accctctcga aaatctgtag tacctaaacg accgacctgt tgatcgtaaa accttgatct
    21721 atctgtaaaa ttaatacttt gcgtcattag gcgcacggta acgggcgtaa gttggcggcg
    21781 atggttgcgg tccgttatcg taggacttga catttctaga agaaaggaat atgtcctgcg
    21841 tgacatatgt taaaaaaagc atgcaaatat taaacaggag gaacggaaat gtcattagtg
    21901 acggtaacca tcgataatcg gcaggtgcag gtggaagcgg gtacgaccat cctggaggcg
    21961 gccaagcggc tgggcatcaa gatcccgacc ctgtgtgcct gggcggagat cggacacgct
    22021 ccgggggcct gccgggtgtg cctaacggag gtggaggggc aacgaagcct gactgccgcc
    22081 tgttcctttc cggtgacgga gggaatggtc gtccggacga atacggaacg ggttcgggtc
    22141 gcccgccggc tggtcgtgga gcttcttctc agcaaccatc ctcaggagtg caacttctgc
    22201 gtccgcaacg gcaactgcga actccagcgg gtcgccgagg ccgcggggat caagcggctc
    22261 cgtttcgaac cccccgaatt ttcccgcgag ggcatgattg attactccag cccctccctg
    22321 gtccgggaca gccgtaagtg catccattgc caccgctgcg tgactgtatg cgaacagata
    22381 cagaccgtcg gggtcctgac gccttccttc cggggcagcg atgtccgggt agccccggcc
    22441 ttcggccttc ctctggacga gtccaactgt atcgcctgcg gacagtgcgt catggcctgt
    22501 ccggtgggcg ccctttatga gaaggatgat gtagatgccg tctggcaagc catcaacgat
    22561 ccgtccaagc acgttgtcgt ccaggaggcc ccggccatcc gctccgccct gggagaggaa
    22621 ttcggtatgc cccccggctc cctcgttacg gggaagatgg tcacggccct tcggaggctc
    22681 ggtttcgaca aggtcttcga caccaatttc accgctgacc tcacgatcat cgaggaagga
    22741 cacgagcttc tcaaacgggt caaggaagga ggcgtcctgc ccatgatcac ctcatgcagc
    22801 cctggctgga tcaagttctg cgaacatttc tatcccgacc tcctccccca cctctccacc
    22861 tgtaagtcgc cccagcagat gttcggtgcc ctggccaaga cgtattacgc cgcggaggcc
    22921 gggatcaatc cccaggatat cgtctcggta tccattatgc cctgcaccgc caagaaatat
    22981 gaggccgggc gtcccgagat gaccagcagc ggctaccgtg atgtcgatta tgtcctgacc
    23041 acccgggaat tggcccggat gatcaaggag gcggggatcg acttcgtcaa tcttcccgat
    23101 ggcgagcacg atgcccccat gggagaatat acgggggcgg ggacgatctt tggggccacg
    23161 ggtggtgtca tggaggcggc cctgcgcacc gtctatgccg tggtgacggg gcagaacctg
    23221 cccgatctga acattaccac cgtccgcggc ctcaccggag tcaaggaggc ctccgtcgag
    23281 gtcggtccct tggggaaggt gaagattgcc gtgactcatg gcctgggcaa tgcccgccgg
    23341 ctcctggaac ttattcaggc agggaagtcc gactacgcct tcatcgagat tatgtgctgt
    23401 cccgggggct gcattgccgg gggcggggag cccatcccca cgacggacga aatccggacc
    23461 ctccgggccg aggccctcta ccgggatgac ggcacggtcc agaagaagcg ccagtcccat
    23521 gaaaacgaat ccgttcagaa cctttaccgg aagttcctcc atgaacccct gggtcataaa
    23581 tcccatgaac tgctccatac gcactacgtg cggcgggggg agaatgtccc ccataaatga
    23641 ttccccccgc gccgtgaaga agatgcccgg caaccttatg gcccggggat ttaaaagacg
    23701 atgaacgaaa ccccgaaggg caatagaacc cacatcgctt tggtcggccg taccaatgtc
    23761 ggcaagtcga gccttctgaa cctcatatcc ggtcaggatg tggcaattac ctccaacgtt
    23821 ccgggaacga caacggacgt tgtggaaaag accatggagc ttctccccct tggaccggtc
    23881 gtttttctcg acaccgccgg tttggacgac aagaccgatc ttgccgagct gcgaaggaaa
    23941 aagacctcac aggccctgaa acgcgccgat gcggtcattt tggtagccga ggcgggcgtc
    24001 tggacggaat atgaggaaag gatcgtgcag gaaacggaaa tggccatgat tccccttgtc
    24061 attgccgtca ataagattga cctggggagt cctgacccga actatctcgc cattatcgga
    24121 gaaaaaacgc cccgactgct ctccgtcgcg gccacgaacg ataagggacg ggaggcctgc
    24181 ctgagagagt taaagcgcca tctagcggcc tccatgtccg gcgaagcggc tgactccccc
    24241 tctctcttgg gtgatctcct acctccggga gggctagccg tcctcgtgac gcccattgat
    24301 cttcaagccc cgaaggggcg cttgattctg ccccaggtgc agaccatccg ggctgccctg
    24361 gacatcgatg cggcggccct gatcatcaag gagcgagaac tttctcacct cctgggacaa
    24421 ctgaaggagt cgccacgact cgtggtctgt gattcccagg ctgttctgaa agtaaccgcc
    24481 ggtgtccccc gggaaatacc ctgcaccact ttttccatcc tttttgcccg tcaaaagggg
    24541 gacctcaacg tcgccgccgc cggtgcggcg gccatcaata gcctgcggcc gggcgaccaa
    24601 gtccttattg ccgaggcctg ctcccatcat cctctggcgg acgatatcgg gagggtgaag
    24661 atccccaggt ggctgcgcca gtatgtgggt ggggagatcc aaacggaaat caccgccgga
    24721 aaagactatc cgcgggatct ccggccgtat cgcttgatcg tccactgtgg ggcctgcatg
    24781 ttcacccggc gggaaatgct ccgccgcctt aaggaggccc gagagcaaca ggtgccgatc
    24841 accaattacg gtctggccat ttccgttacc cagggggtga tcgaacgaac cctgcagcct
    24901 ttcccggcgg cgctggcagc ctatgaacgg gagctggccg ccccggcaag caatatcaac
    24961 ggcatgcggg cgcaatagga ggaatgatga aggatttcat cgatgatgaa cttatagagc
    25021 aattattgga agcgacccgt cgtcccaccg ccgcccgggt acgggaggtg atcggcaagg
    25081 cccgggaact gaaggggctt tccccacagg aaacggccat tctccttcag gctgaggacc
    25141 gggatctcgt cagcgagatt ctccaagcgg cccgagagat caaacggatg atctacggca
    25201 accgcctggt cctcttcgcc ccactctaca tcgccaatta ttgtgcgaat aactgtctgt
    25261 actgtggatt tcgccggggt aacaaggaat tgaaccgggt ggccctgact atgggccaga
    25321 tccgcgggga ggttgaggtc ctcgcgaggg aaggacataa acgcttgcta atgctctgcg
    25381 gtgaacatcc cgatcgttcc gggctcgatt atttcctgga agccatcgag acagcctacg
    25441 gggtcaagac ggaaaagggc ggtgagatca gacggatcaa cgtcgagatc gctccccagg
    25501 aggtggaggg ctaccggcgc ctgaaaaagg ccggcatcgg gacctacgtg ctgtttcagg
    25561 agacctatca tcatgagacc taccggaaga tgcaccgcga cggtcccaag gccgactacc
    25621 tatggcggct gacggcgatg caccgcgccc aggagggagg gatcaacgat gtgggcatcg
    25681 gcgccctctt cggactctac gactataagt ttgaggtcct gggactgctc atgcatgccc
    25741 ggcaactgga gagggattgc ggcgtgggtc cccacaccat ctccattccc cgcctggagc
    25801 cggccctcaa tgccccggcc gcgatgacgc cgccctgccc cgtgagcgac gaggacttcc
    25861 agcaactcgt ggccgtcatc cgtctggctg tgccctatac ggggatgatc ctcagcaccc
    25921 gggagacgcc ggccatgaga agagagatct tcgaccatgg cgtatcccag atcagcgccg
    25981 gctcccggac caatcccggc ggctaccggg aagggacgag cgacggcttc cgggctgcgc
    26041 agttcaatct cggtgacacc cgcacccttg acgaggtaat ccacgatatc agcacccatg
    26101 gacatatccc cagtttttgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
    26221 ccttgacgag gtaatccacg atatcagcac ccatggacat atccccagtt tttgcacggc
    26281 ctgctatcgt ctcgggagga cgggtcagga tttcatggac ctggccaagc ccggcctgat
    26341 ccaaaggttc tgccggagca acgcgatctt cactttcatg gaatacctgg aagattatgc
    26401 cgccgaggag acaaagaccg cgggaaaaaa gttgatcgcc cgcctcgttg ccgagaccga
    26461 tccagcgggc gtccgggaaa ccctgaccgg acgtctggcg aggattgaac atggagagcg
    26521 cgacctctac atctgacggc gccgggatgg aaacaggggc cgccattgcc gatgctgtcc
    26581 gaaacgggga actgaccaga gaagggattg ttcggctcct ggagattgcc gatcccgaag
    26641 agcttcagaa gctttttata gccgccgatg ccattcggca acgctttgtc ggttccgagg
    26701 tgttcctgcg ggggattgtc gagttctcca atttctgccg tcgtgactgt ctctattgtg
    26761 gactgaggtt aagcaaccgg gatttgcccc gttaccgaat ggaggaagga gagatcctgg
    26821 ctgccgccca ccggataaag gccgccggta tctctactat cgtcctccag tcgggagaag
    26881 acccctgcca aagcaccgtc cgtatctgcc gcctgatccg gaggatcaag gatgaaactt
    26941 ccctggtggt taccctttcc gtaggcgagc ggccccttgg ggattatctc gcctttcggg
    27001 aagccggggc gaaccgctac ctcctgaagc aagaaacatc ctctcccgag ctatatgccc
    27061 ggttccgtcc cggatgtcgt cacgaggagc ggcagcagtg tctccgccat ctgaaagaac
    27121 tgggctacga gattggcacg gggaacatcg tcggacttcc cggacagaac accgccgacc
    27181 ttgccgccga cctcctcttt atccgggaga tcgacgccga tatggtgggg atcggtccct
    27241 ttatcgctca tccccaaacg ccactggctc cctgcccgaa cggcagtccg gcgatgacgc
    27301 tgaaggtgct ggcggtggcc cgcctgctca ccagatcgac gaacatcccg gcaactacgg
    27361 ccctggcaac aatcgcaccc caggggtggc gcgatgcctt gcaatccggt gccaacgtca
    27421 tcatgcccaa tttcaccccg gtcgtttata ggggactcta cgagatttat cccggtaaaa
    27481 gctcagcatc cacaaatgtt cccgactttc cggaaatgct ggagcaggag ttgagggaca
    27541 gcggccggat catcggcacc ggcagtggct ttcggaagcg gcaggaaatc tcgggataac
    27601 ggatcatctc cgcagcgatc agggttccaa ctccattacc agttccccgc ttcgcgacgg
    27661 gtcatacccc ccgagggccg cggccctgtc gcgaaacttc cgagagcgga tcgtttccag
    27721 gacggcctgg atattgggat cttggagata agcggtgggg ataacgaggt cgtattgttc
    27781 tctgtccaaa ggaatgaagt ccaggtcaag ggcccgggcg gcggcgtaaa tggccatgcc
    27841 gcaatcggcg gcgccgctca atacatccac ggccacggac atatgggtaa attcttcatg
    27901 accgtaccct ttaacggctt ccgggctgag gccggaacgg accagattgt aatccagcaa
    27961 tatgcgtgtt cccgagcctg cctgccgatt gacgaaggag atgtcgttcc gggcgaggtc
    28021 catgattctt ttgatgccct ttggattgcc cttttgaaca atcaacccct gctctca
//
LOCUS       DAOSMF010000016        27778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_2211_length_27778_cov_16.751542, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000016 DAOSMF010000000
VERSION     DAOSMF010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 27778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27778
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2211_length_27778_cov_16.751542"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="PLT63_03455"
     CDS             <1..456
                     /locus_tag="PLT63_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97811.1"
                     /translation="AKVAVANGTGQSCQLYYAEYNTVAGGSFAITASTPTTLTLAGSP
                     TDGTCTFWLHKTIVRDITDGMTSGHKILTLRDSDNDLNAFALPATADGTGAAAYGNDG
                     YYFDKVAARAALRGGYFNITAIAGVFFLDLSRAPSNATIAIGFRAAKAL"
     gene            464..775
                     /locus_tag="PLT63_03460"
     CDS             464..775
                     /locus_tag="PLT63_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97812.1"
                     /translation="MSWLSDIFGIFRKRGIMGVCRHDATYCMMVYGEHYPARVAVGWR
                     GGQYHAQAQAKIDGVWTWLTSSLGTVYPTKEDPTFRVDLFATPDAWWAGNKNLLVKEE
                     I"
     gene            1118..1468
                     /locus_tag="PLT63_03465"
     CDS             1118..1468
                     /locus_tag="PLT63_03465"
                     /inference="COORDINATES: protein motif:HMM:NF024929.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HOD97813.1"
                     /translation="MGLRERQSIFARNAARLIEWCFRNGYEVTLGEAWRPQFAADEYA
                     KQGKGSKNSLHTERMAIDLNLFKDGKYLTNSSDYSRAGDAWKALHDMNRWGGDFKKLK
                     DGNHFSMSVGDGRA"
     gene            1542..2105
                     /locus_tag="PLT63_03470"
     CDS             1542..2105
                     /locus_tag="PLT63_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97814.1"
                     /translation="MKRLLWIMVLVLIGCGPTANQLAGEKAFYEAKIALSKAASSQPV
                     FEMVSGDPTKPIILNNVSAIRVFQIPVSANGDGLQQYQMRDYTEPWLRVIQTAVGTAA
                     PWIGAWGIVHEVGKAAGATTSYSTNVSGTGNSATTTAIGSMGNITGNQNAVATQAADS
                     LNTPTTTTVTTTNTDDHTVTTTVPEAP"
     gene            2102..2314
                     /locus_tag="PLT63_03475"
     CDS             2102..2314
                     /locus_tag="PLT63_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97815.1"
                     /translation="MKELILNRLREASTWKAIFSILSGLGLFTLTDAQADAAAAMMIA
                     VYTFLSLILPDKFGKKDEKPDNGPKD"
     gene            2413..2646
                     /locus_tag="PLT63_03480"
     CDS             2413..2646
                     /locus_tag="PLT63_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97816.1"
                     /translation="MKILLLIVQLIPALISLIKAIEDALPAAGLGAEKLAAVRKIIEA
                     TFEGASEIWPVVEKVIGVLVSLFNSTGVFTTDK"
     gene            2655..3632
                     /locus_tag="PLT63_03485"
     CDS             2655..3632
                     /locus_tag="PLT63_03485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HOD97817.1"
                     /translation="MQAVIKTAPTIEPISLADLKMHLRLDSETLDGNLALTQSLAYGS
                     HGISAGGLYTHTGTGVDVLGKQAEVLVHHGTNGATGTVDTKIQEADALAGPYTDWTGG
                     AFTQVTTDGAGATEPDNADYKKQYTGSKQYIRTASKVLLAACEFGTSILVNAATVAED
                     DLLTAIIQASREYVENYTRRALLTQTWYLYLDAFPDCDYIKLPWGNLQSVTSVKYKDY
                     EGTETTMTVTDEYLVETNGEGVGRIVLPYGVTWPTFTAYPSNPITIEYVCGWTAAALV
                     PEAIRAACKLVCADLWTNREGQSVGSAQIYFENPVVQRLLYSHRLWDEF"
     gene            3695..4015
                     /locus_tag="PLT63_03490"
     CDS             3695..4015
                     /locus_tag="PLT63_03490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HOD97818.1"
                     /translation="MSAGILNKRIILEAPVKTSDGMGGFSESYSTVAEVYAAIWPVSA
                     TDILRNNAPVMEVTHRIRIRYRSVMKSAWRISWAGRYFSIVSIIDPNMRHEFLDLLCK
                     EATG"
     gene            4012..4422
                     /locus_tag="PLT63_03495"
     CDS             4012..4422
                     /locus_tag="PLT63_03495"
                     /inference="COORDINATES: protein motif:HMM:NF022810.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HOD97819.1"
                     /translation="MKNLSTAIYTQFGTSTLSTYIGGRLYKGMAPDGVEFPYAVYSLV
                     SDVPDNVFAKQGEEAIIQFDLFSILSSSTQVETMYDYLKTAYDDCSLTITSTTLIWFR
                     RDNAMLMVEDYTTPAGTQKVWHYAVDYTVILVAS"
     gene            4479..5162
                     /locus_tag="PLT63_03500"
     CDS             4479..5162
                     /locus_tag="PLT63_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97820.1"
                     /translation="MKNKNIIVVQSLIRELGEESLRNPDVVADLIRAFGIVQWGPDVF
                     GADEVFKNPAESMAGIYQTPCQLARALVFLSELKIGSYLEVGVFQGGCFLFVSEYLRR
                     FNPKIVCLGIDPTNYLNPEVREIIDVADWMKHASITSDHIAGRKHDLVFIDGDHGNGW
                     PARDWNNVGKHAKVCMLHDIQGPSCPDVVALWEELKKDKKRSWVEFLEHNAPAPLQGI
                     GIIHERGKA"
     gene            5258..6511
                     /locus_tag="PLT63_03505"
     CDS             5258..6511
                     /locus_tag="PLT63_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97821.1"
                     /translation="MFADETIEFGEPWIRPYVIDEPSKLCTVLMPGTYKRNRWIEAHE
                     LEPEDYYLTVDDDDFYEPGAFEEVRKEDADIVIISMKRGVQVPEVASPERAYPTSTLV
                     ASPENMRIGRISAQQMFVKGKLFKTHPHNEESHCWDGELAEHYQASHKNTVFLSQVYA
                     LFNYFEPGRWTPGLKVSFGVMTNLPSRLSMSLQQSEIVGDLHYVSNPKSATKGLNKLL
                     DRIAVDGSDVAILTHHDMSFRKPWLPKIKNELAALPPDWVVAGIIGKDMEGRICGKMS
                     DSRIPVLFNTSDIHEFPVKACCFDECLIFVNMKSGFRFDEALDGFDLYGTLCVLQTWE
                     MGGSAWIIDSGAYSIMADTEHGNMQVDVALATHHCTRPFTWFPDNKFQKNFKWLYDRF
                     TTAERLDTTVLGLPPEDKRFETSAA"
     gene            6536..6964
                     /locus_tag="PLT63_03510"
     CDS             6536..6964
                     /locus_tag="PLT63_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97822.1"
                     /translation="MAKVCGKGGKVMYGSVVVANIKEWSMSGFSVETVKKDPAFGDTV
                     IEYCTTGLSEPGTISFTGNYDPADTNGQIALAAVAAAGTALTNLYLYANTSTFWRVGS
                     GGSILVTKANAISLGRTGIGTISFEGLVQGANMEQVGTGS"
     gene            6989..7444
                     /locus_tag="PLT63_03515"
     CDS             6989..7444
                     /locus_tag="PLT63_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97823.1"
                     /translation="MLIDLSRDQGEWFPFQQSHIDPITGEPIFDPPASEAAVQVRSLA
                     PFYEARIAKRERKVEHVYNPKTRAMERISYIEELSPAAARAEAEDAFDYAITGLKGFE
                     DSRTGEELKCDRATKIALMQVPVFDRFIARCFKLLAASGIKEREDTEKN"
     gene            7858..8283
                     /locus_tag="PLT63_03520"
     CDS             7858..8283
                     /locus_tag="PLT63_03520"
                     /inference="COORDINATES: protein motif:HMM:NF016754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HOD97824.1"
                     /translation="MSCKITYDFKDLGVIEERIEKNLMDRLEAAGELIATRARQLCPV
                     GTTRRPPYKNGQPWTAREPGTLRGSIRVTRLKGDPNLDVRVYAGARGKTNEGYYAHMV
                     EYGSIHNEKKSFLRPAAKEIGKNIETIMTSGGDVTGGME"
     gene            8287..11937
                     /locus_tag="PLT63_03525"
     CDS             8287..11937
                     /locus_tag="PLT63_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97825.1"
                     /translation="MAANAGKIFVQVELDDKKFKTSLANMPSDAKATASGIEKVWKTL
                     GTQSEASFNAQRTAYKNALTLIKNDVNSSKADIVRAEEAAAAKIKAIDTQQYGERKSA
                     ITNLKEHWLAATAAVAGAIALANKAWSYAEAASQYQQSSSAFRSMAQSMGKDATTEFN
                     KIKVASAGMIDDKALTESANKAMSLGIPIESLSSLMEIARAKSRDMGTTTQQAFNDIA
                     TGVGRASPLILDNLGLSLKLGAANEAMAAKLGKTVAELTDQEKKTAILNATIAAGSEA
                     LTRYDLSQTTTAEKMQQLTTTIANLKLDLGAGIIRAAAGAYGALQTLATGALVAAAGF
                     YKLQQASLTVQSWTSFGDAAKAYAAAAKEAGENAKAAMGASEELAGKALDSFTVMTAS
                     ADEVSVATRGIGTASTEAAGVDEKAKKKQLEAAQKLEEQIRETNRKIALSAIEYAAQE
                     DEEREKYLKQYEEADKKAAEDEIKRWQKASEAAIDAMKEEAEEGAKQSKATIERMAKE
                     EKAALDLADKQLNAAKSMYSDMDDYSEEYYETVKELLKKEADARLKILLQGVTDTEEA
                     DALRKASAAKLTSDLEAEEIKRLKASKDWKDGVTAYFKEAEKDCKTTAEHMYDFMKTF
                     ADASKTAISDTLFDAIKDGTVDLEDIWETWSDSLLKKFTDTVAEMATDFAVDGIKAMV
                     SAAKEPISMVFSAAWDAASAIVLAGIKALSKWFNMAYSGGYVRDSGIGAYADGGTVPG
                     YATGGDSPANDTVLALLSPGEYVMPRSAVNAETLPHLEYMRDNKRPRGYAYGGPVDAL
                     PGLSKLYGDRYGQAGVWYDEDEWGALADAYKNLHDYIGMSYYNGKWGFIKEGWDDSFG
                     AYPKEFSTVADPVSYTGDWFNRNKYGSSLQSVDGGALGYDDYKYLLDWIIGSVHGIAY
                     GYGEGGEPDPYEGLRNALTGSLDPNVVWVEPEGSGYRWYMRDGSEKYTEPSNNFFDRF
                     MPSIIKAVGAVAALVIATAAALPTGGQSYTLLSGIVAAMEGGMASAPALIGAAGTGLY
                     SGLVTYGETGSVTGSIIAGIIAAAATALSFGSPWGQSETGLYVLKSELNASGYLTSAQ
                     GRIIADVIRKGAGIVLEDTLNSVVGGGGSMAISGKGDPGGLDTLAGLFENLPFNKEVS
                     LASGIDYVPYDNFPAKLHEGEAVLTKEENKKGRGITVNFNLTGSVIDKQAVNEFAKAI
                     YPRLKRLEAYGY"
     gene            11937..13028
                     /locus_tag="PLT63_03530"
     CDS             11937..13028
                     /locus_tag="PLT63_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97826.1"
                     /translation="MGKARFIYQNHIPDEDAFTVSSLQPGQVTAALKDGSGSASITVS
                     GSFTGAVDKEYVIEIDSIAGGAEVGQATFKWSDGGGSWNASAVTTPSAPTELNSGVKV
                     AFSSGDGADFVVGDRWYFKGINLFNAGKLIDLNRDSRYRSGSLEEPNTIDADMGSDQS
                     VLVVVIFDHNFTSGAAISISGDSASTFDSGIGGAPEYSESIVWNEDGIVHYMTTPSTY
                     RYWRLSVEDTSNSDGYIEIGELLIGSYAELSRNYNYEFSRSQGFIFTQNETAMGIRRA
                     RYYNARRIIEINQTYLTLSDISKLENMAETLNSKSAGTMKPFYFNFDSAVPNDTFMVQ
                     LTGLARERAGTAAELYHSPMTLEEVIKSD"
     gene            13021..13527
                     /locus_tag="PLT63_03535"
     CDS             13021..13527
                     /locus_tag="PLT63_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97827.1"
                     /translation="MIRIPIAFHKNLLRGEIPIAYVMIITHLANRIYSDRELKGIFAA
                     AGCLADGTYLADGSILAGAQSTGGVEKSARVLDFGKFERSIVPQTSDLLLAYTGKQIQ
                     NNQVVLDNTDNYFTRMIAQEPFLTRELRILIGFEDQPQDDHFPLFAGIITDIEVDENS
                     MTIEAGEM"
     gene            13529..16462
                     /locus_tag="PLT63_03540"
     CDS             13529..16462
                     /locus_tag="PLT63_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97828.1"
                     /translation="MAYTLENGNIVKAWASSCYGSAHMPLNAFYTGTPLTGGSNWRSY
                     ATPVGITSYSLTCQFNSAKVITYVWYSNFFDCYDDDTTYYPGSNTSGSAGFTLYGSND
                     NSNWTEITSGTFAIHPAANVASGGEFAFSNSTAYTYYLFYVSASHGGHLAAIRRLQLY
                     SGSTTPPTSVPTVTTTSISGILATGATGGGNVTSGGGATVTARGVCWNDTGSPTTADS
                     KTTDGTGTGLFVSTLSGLTKGKKYYVRAYATNSQGTGYGSQVEFTTLDVPKVTTAAAT
                     DITGISAKVSGAITDTGGENASTIGICWNTGGTPTTADSKYSKTSYKAYGVGTFSGAP
                     YTASPLLPKTKYYVRAYAINSVGTGYGNEIELTTLAFPTVTTTAISSIAGTTASSGGN
                     VTNEGDESVTARGVCWNTTGSPTTADSKTSDGSGSGAFVSSITGLTRGVTYYVRAYAT
                     SSVGTAYGEEVSFTTLTVPTMATKSVKNITKATALVECEIVAIPGAAKCTTRGICWST
                     SANPTTADSKAFDNGGGTYDVGGYTKTASGLSPVTRYYVRAYAINSEGTAYGAQLSFV
                     TLSKAATIAPQYKLTDTYYLGRTGRYSLPQNSNDRLPLVYGDLTDSSEGNWKLPCIDT
                     NTYVYCFAAHEVLSVANGNSITIYENGVEVDSGDYAFDESNDYEGEGAIATVTFSTPK
                     TGSTITARGMGKPTTSGGATLMENIIDILEDVLTVELDMDIDFEETYKATARDDFEGQ
                     SYVAAGVIYKDIKIWDLILMMMGSFLGSAYMNGAGELVLEIDDGTYPPESFSGIVSKS
                     DAVFRSASMSLSNLINRCPASYAYDYATGNDFKNHSDGVTTASSASEHIYGVREPTLP
                     YKFYWCRDAASVAVMQQIIVDKFAYPLWTIEFDDITMKRLDSDVGKLIAYSVDRLYDK
                     DMAAMLNTLWKVVSYNPDIQRGSIGFRAIETGFYLTHAYIANGDWIADGSIKAGNNRD
                     VTNYG"
     gene            16475..17071
                     /locus_tag="PLT63_03545"
     CDS             16475..17071
                     /locus_tag="PLT63_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97829.1"
                     /translation="MADQIIQATEEMVGAGHATKADTLNRAFLIEHNTDGTHEKLSVG
                     SDANGDMYYRANSVLARLAKGTANYKMFMNAGGTAPEWAMGIKTISSSYDLSTESGDQ
                     SITGVGFRPCAYIFLGTISADVVWNIGFYALAAYSIQFRGDTSKMLSGPGYISRIYLS
                     SGSVNTVPKSIESDGITITHTKSGSPTGTLVQYWMFFR"
     gene            17080..17436
                     /locus_tag="PLT63_03550"
     CDS             17080..17436
                     /locus_tag="PLT63_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97830.1"
                     /translation="MRICIRKSDKRIIEMQSNATEGTLITNALTAGYTLDEIEEREVD
                     EAGYADALAEDPVEIAAKAKAEADASAQAAKTVAIADAKDSISTIETAVEKAADLETL
                     RKNVLELVKAVKVLTA"
     gene            17812..18051
                     /locus_tag="PLT63_03555"
     CDS             17812..18051
                     /locus_tag="PLT63_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97831.1"
                     /translation="MGTKANSRRKFNRNLFYGKLHTDGLTLEKLGQQLNPPISRFRAC
                     QIVNKGKPAHRLQEIASILKTNVQTLFPKEVGNAE"
     gene            18041..18244
                     /locus_tag="PLT63_03560"
     CDS             18041..18244
                     /locus_tag="PLT63_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97832.1"
                     /translation="MPNDAMSDIDKLENIFSLRIPSATKRMLDDLTTVEKAKLNEAVR
                     NTIARAIHESKFDPRLYLRDASE"
     gene            18287..18781
                     /locus_tag="PLT63_03565"
     CDS             18287..18781
                     /locus_tag="PLT63_03565"
                     /inference="COORDINATES: protein motif:HMM:NF024784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HOD97833.1"
                     /translation="MKMIPLTNGQKAIVDDEDFDKVSAFNWFLVHERNGTKRARRTTP
                     DYIDMARIVLEVPGGFIADHINGNPLDNRKANLRLASKQQNLRNRGKGLWLGRSSSRY
                     KGVCFLRGKWQAQISKDQRAIYLGTFPCQFCAALAYDIAAVEHFGEFARPNFLKAPKG
                     SSAA"
     gene            19041..19187
                     /locus_tag="PLT63_03570"
     CDS             19041..19187
                     /locus_tag="PLT63_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97834.1"
                     /translation="MTELALCLILISVLVLAVCILHAAGVMLVRWRSYRTGKREYRIV
                     QRVL"
     gene            19326..19523
                     /locus_tag="PLT63_03575"
     CDS             19326..19523
                     /locus_tag="PLT63_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97835.1"
                     /translation="MAPQARTTQDQLEWDQFKSQIETMLKAFGTLLFLPLILLAAMAV
                     GLREGIVAGAKASLSAIERWC"
     gene            19525..19785
                     /locus_tag="PLT63_03580"
     CDS             19525..19785
                     /locus_tag="PLT63_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97836.1"
                     /translation="MSTKHFESARDAEIKRLKTELAKYDTPRDETTGLRIKIVELQKE
                     LAELKAEIERLKKPKGFHDVSDLYDDGITGRQWMIEQEASHE"
     gene            19778..20155
                     /locus_tag="PLT63_03585"
     CDS             19778..20155
                     /locus_tag="PLT63_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97837.1"
                     /translation="MNDTRRKMLTEYLGSCWHEIVDKGPYLSTCSKCGMTFGAIHTSD
                     WDPAGFNRTFATHADLHALVCKMKEKQELWEFEQIFALKRWHDYTSNGETYLSWLITD
                     PARTCELIAEWMEAKHGHDNKDA"
     gene            20570..20818
                     /locus_tag="PLT63_03590"
     CDS             20570..20818
                     /locus_tag="PLT63_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97838.1"
                     /translation="MKLYRIKLRGMCGGLTTQVAYGCPYVVAESPNKALEIVQSYLSK
                     KDIGFSDEREMVNIELLAEQADYPSCKIQLFIQGIEAQ"
     gene            20885..21010
                     /locus_tag="PLT63_03595"
     CDS             20885..21010
                     /locus_tag="PLT63_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97839.1"
                     /translation="MWVRIVKVAVAWMLRKHKWMIYEMIVPDGYHVHKNPEKVKA"
     gene            21007..21261
                     /locus_tag="PLT63_03600"
     CDS             21007..21261
                     /locus_tag="PLT63_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97840.1"
                     /translation="MSAIDDIRERVRMGLHLENADAELLLDELDASNIRCLQYSRLVG
                     ETRGKIQAMIMEMERGQEARIRDLAARRTAAKEAIERGGV"
     gene            21258..22121
                     /locus_tag="PLT63_03605"
     CDS             21258..22121
                     /locus_tag="PLT63_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97841.1"
                     /translation="MNELQKYRRPLTAIDIKAQVQLIQEVMKAVMEKDVHYGIIPGTP
                     KPTLYKPGSEKLLSTFRIGVEPETIDLSTPDEVRYQVKARGFSQETGALLGVGIGECS
                     SNEEKYKWRRPVCDEEFNETPEDRKRVVWKRGQQKNYQQKQIRTNPADMANTILKMAK
                     KRAQIDLTLTVTAASDIFDQDLEDLPAGVVEGIVEKESEKKPPVATPQKKAKDPNEPA
                     TEPQIKAIHALLGKLGIKDDFEKHMRVSRIIGIPEPEVITSMSKLTKGQASTVIETLT
                     KEGSNEGDFGH"
     gene            22099..22650
                     /locus_tag="PLT63_03610"
     CDS             22099..22650
                     /locus_tag="PLT63_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97842.1"
                     /translation="MKAILDIKSGHVEPWTSLQLAAYTLLDAPVEFESDGHRYGNGLE
                     SVTQILKAEQLIDTRFYDDWSRDRGSMIHLATHYDDMGELDEETLDPVIRPYLEAWRK
                     FRKESGFVPEVIEVPMMSINYRYAGTPDRIGQLPGKIKRAAVELHDDGGYRLIPYTDR
                     QDVHIWLSVLAVHNWKRNNLKGR"
     gene            22655..22795
                     /locus_tag="PLT63_03615"
     CDS             22655..22795
                     /locus_tag="PLT63_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97843.1"
                     /translation="MMDDFQLLQFIQETKERILALEARVHALESWRDEDETYKMEQKE
                     RE"
     gene            22965..23627
                     /locus_tag="PLT63_03620"
     CDS             22965..23627
                     /locus_tag="PLT63_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97844.1"
                     /translation="MSLVETTPVEEKALSIVDQAKAVKVTDAQTYTAAGSLWKSIGEM
                     IKEVKDTFDPICDAAHKAHKQAVEKRAKYLDPLTAAQKSVKGLMSAYDQEQECLRREE
                     QARLEAIARKAEEDRRLQEAIEAEAAMKAQGASQEEVAQETAAILETPVSVAPVVLPK
                     ATPKLQGGPVYRTVWKARVINERLIPREYLVPDMVKINGVVRSLKGAANIPGVQAYEE
                     RC"
     gene            23629..23910
                     /locus_tag="PLT63_03625"
     CDS             23629..23910
                     /locus_tag="PLT63_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97845.1"
                     /translation="MIGPKTIESMKSLAAEKIDTYAEKMNESFLKSDDGKLRVTLAFE
                     LSMSEKPDSVDLDCTISFTSEKIKDKTSTTITEAQAELPLADKVYRLGK"
     gene            23907..24749
                     /locus_tag="PLT63_03630"
     CDS             23907..24749
                     /locus_tag="PLT63_03630"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOD97846.1"
                     /translation="MMGMTSLFNTDCMEAVKKMPAEVYSKTQALRHKLINDHFQNYKT
                     YQIPKAQLVIADIPYNVGKNAYGSNPAWYIDGDNSNGQSDLAGTSFFDTDKDFRISEF
                     LNFCARMIIKEPKETGKAPAMIIFCAFDQQMELIEKAKQWGLNRYINLVFIKNFSAQV
                     LKANMRIVGNCEYAIVLYRDKLPKFNNGGKMIFNAIPWEKDGKSEKIHPNQKPVKLLK
                     KLINIFTDPGDVVIDPVAGSGSTIIAAIQSGRSAYGFEIKKNFYREASKWIERELKQQ
                     VLPL"
     gene            24799..25122
                     /locus_tag="PLT63_03635"
     CDS             24799..25122
                     /locus_tag="PLT63_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HOD97847.1"
                     /translation="MWRNQKKLTPEGQLTASVRSLLRAAGIFHYKHWGGPMGYPGVAD
                     IIGCYKGRMIAIELKSPTGKPTPEQERFIQNVNDAGGIAFVARSIDEVIEGLNLQDRF
                     LRFGR"
     gene            25126..25962
                     /locus_tag="PLT63_03640"
     CDS             25126..25962
                     /locus_tag="PLT63_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97848.1"
                     /translation="MGKQPAFQFYPGDWTRDLDDQDLETEGAWIRIICRLWWSETRGE
                     ATKSIGEWSRILRKSEKKTMKIFQNLIAKRIADGCLLDNQNITIISRRMKRMVEISQI
                     RYQVGLKGGNPALMKTRNNLVNQTSNQNARSSSSTSSSIKKESIKKEKIQFINNKFQT
                     IPDALITKWREVAPGIIINDEIKKAELWLLAHPEKRRSRYDVFLSNWMVRAQERFIKY
                     GGGNGRTKPDFTGDKRESVQTKIDADLAKANALYAAAKAAAAFRAGGDAGNDDAPDFQ
                     NG"
     gene            26313..26504
                     /locus_tag="PLT63_03645"
     CDS             26313..26504
                     /locus_tag="PLT63_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97849.1"
                     /translation="MKRNLAPFLILAFALYGFWRLMFDWFGSYIRQACVYIDGMGAAF
                     WVCLAAAGVIVVVVHRRVH"
     gene            26528..26779
                     /locus_tag="PLT63_03650"
     CDS             26528..26779
                     /locus_tag="PLT63_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97850.1"
                     /translation="MAGNVMPMMVPRVGGQQQMFDITEAVPKVCDKCKGEHFDKVYRL
                     GMIPKMAIRNKTGQEIRVEYQTFLCRSCGHEFGVPVVVQ"
     gene            27355..>27778
                     /locus_tag="PLT63_03655"
     CDS             27355..>27778
                     /locus_tag="PLT63_03655"
                     /inference="COORDINATES: protein motif:HMM:NF014273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HOD97851.1"
                     /translation="MVEISIKTVKLSTIKLNKENPRRISNQDMERLVKSLQDFPEMMK
                     LREIVVDETMTVLGGNMRYLALKKIGAKECTAKIVTGLTPEQKREFVIKDNSQFGEWD
                     MDLLSAWDDLPLVDWGVDLPDDWLNPGGGDGGQQTGSGN"
BASE COUNT         7332 a         6626 c         8089 g         5731 t
ORIGIN      
        1 gcgaaggtgg cggtggccaa cgggaccggg cagagctgcc agctctatta cgcggaatac
       61 aacacggtcg cgggcgggtc gtttgcgatc acggccagca cgccgacaac gcttaccctc
      121 gccggttctc cgacggacgg gacctgcacg ttctggctcc ataaaaccat cgtccgggac
      181 atcaccgacg ggatgacgtc cgggcacaag atcctgacgc tcagagacag cgacaatgat
      241 ctcaacgcat ttgcgctgcc cgcgacggcg gacgggacag gcgcagcggc ctacggaaac
      301 gatggctatt acttcgacaa ggtggctgcg cgggccgcgc tccgcggcgg ctacttcaac
      361 atcacggcga tcgcgggggt gttctttctc gacttgagcc gcgcgccttc gaacgcgacc
      421 atcgccatcg gctttcgcgc cgcaaaagct ctgtaagagg cacatgagct ggctatctga
      481 catattcggc atcttccgca aacgcggcat catgggagtc tgccgtcacg acgcgaccta
      541 ctgcatgatg gtctatggcg agcattatcc cgcaagggtg gcggttggat ggaggggcgg
      601 gcaatatcac gcacaggcgc aggcaaagat agatggtgtg tggacatggc tgacatcctc
      661 attagggact gtgtatccga ccaaagaaga tccgacattt cgggtagatc tattcgccac
      721 cccagatgcg tggtgggcag ggaataagaa cctgctggtg aaggaggaga tatgaaaaag
      781 ctactcgcaa taatcgtttc ggcccttgtg ttggtttcgt gcgggccatc gttgcaccag
      841 attgaggcgg agaaagcgtt ctacgaggcg aaagtcgcaa tagccaaatg aacgtcgttt
      901 tgaaaggggt gacttatgaa cggcgggttt atcgtaagtg cggaaacatg gaaagagctg
      961 accccggaac gtcgggagtg gatactgtat gacacgatgc aacggcttat tgtcgaggtc
     1021 cgatgtttga gaaaatggaa ccgatcaatg gcctttgccg ggggtgccgt tggcggggca
     1081 ttggcggcat taggaatgaa gtggctggga taggaaaatg ggactgcgtg agcgacaaag
     1141 catcttcgcc cgaaatgccg cccgactgat cgaatggtgt ttccgcaacg gctacgaggt
     1201 gacgcttggc gaggcgtggc ggcctcagtt cgcggcggat gaatacgcga agcaagggaa
     1261 gggcagcaag aacagtttgc acacggagcg catggcgatt gatctcaatc tcttcaagga
     1321 cggtaagtat ttgacaaaca gctctgacta ttcccgcgca ggagatgcgt ggaaagcctt
     1381 gcatgatatg aaccgatggg gcggcgattt caagaagctc aaggatggga accacttcag
     1441 catgagcgtc ggagacggca gggcgtgaaa ggcacggcag cgaagctccg ggaacaggcg
     1501 gcgaaagaga agaagcaacg ccgcaagagg aggaaaagaa aatgaaacgg ttactctgga
     1561 tcatggttct tgtcctgata gggtgcggtc ccacagcgaa ccagcttgcc ggggaaaagg
     1621 cattttacga ggcgaagatt gctctgtcga aagcggcctc ctcgcagccc gtatttgaga
     1681 tggtttccgg cgatccgacc aagccgatca tcctgaacaa tgtctccgcg atccgcgtgt
     1741 ttcagatccc ggtatctgcg aacggtgacg gcctgcagca gtatcagatg cgcgactata
     1801 ccgagccgtg gctgagggtg atccagacgg cagtgggaac ggcggcccca tggatcgggg
     1861 catgggggat tgtccatgaa gtcgggaaag cagcgggggc gacaacctct tattcgacaa
     1921 acgtgagtgg gaccgggaac agcgcaacca cgacggcaat cggctcaatg ggcaacatta
     1981 ccgggaacca gaacgccgtg gctacgcagg cggcggactc tcttaatacg cctacgacta
     2041 cgacggtcac gactacgaac acggatgatc atacggtgac aacaaccgtt ccggaggcgc
     2101 catgaaagaa ttgatcttga accggcttcg ggaagcatcg acgtggaaag cgatcttctc
     2161 aatcctctca gggcttggcc tgttcaccct gaccgatgca caggcggatg cggcggcggc
     2221 aatgatgatc gccgtctata cattcctgtc gctgatattg cccgacaagt tcggaaagaa
     2281 ggatgagaaa cccgataacg ggccaaagga ctgacggccc cagagaaaag aaggcaaggg
     2341 tggtctcgat ctcattcact tgggattggg atatgtggct tgaaagactc ttcaaaagaa
     2401 aggggaaagg caatgaaaat cttgttactg atagtccagt tgattccggc cctgatctcg
     2461 ttgatcaagg cgatagagga tgcgcttccg gcggcggggc tgggggcgga gaagctggcg
     2521 gcggttcgga agatcatcga ggcgacgttc gaaggggctt cggagatttg gccggtcgtt
     2581 gaaaaggtga tcggggtgct cgtgtccctg ttcaattcaa ccggagtgtt tacgacagat
     2641 aagtaggtga cgctatgcaa gccgttatca agaccgcccc gacaatcgag ccgatcagcc
     2701 ttgctgactt gaagatgcac ctgcgtctgg attcggaaac gcttgacggc aacctggcgc
     2761 tgacgcaatc cctcgcctat ggatcgcatg gcatttcagc gggcgggctt tatacccata
     2821 caggaaccgg cgttgatgtc ctcggcaagc aggcggaagt gcttgtgcat cacggaacca
     2881 acggggccac cggaaccgta gatacgaaga ttcaagaagc cgatgctctg gccggtccct
     2941 ataccgattg gacaggcggg gcattcaccc aggtcacgac ggacggggca ggggcaacgg
     3001 agccggataa cgcagattac aagaagcaat acaccggatc gaagcaatac atccgcacgg
     3061 cctccaaggt gcttctggca gcctgtgagt tcggaacgtc catccttgtc aacgcggcga
     3121 ccgtggcaga ggatgatctt ttgacggcga tcatacaggc aagccgggag tatgtggaaa
     3181 actacacccg ccgggcccta ctgacacaga catggtatct ctacctcgac gctttcccgg
     3241 actgcgacta catcaagctg ccgtggggca atctacaatc cgtaacctcg gttaaatata
     3301 aggactatga gggcacggaa accactatga ccgtcacgga tgaatatctg gtggagacca
     3361 atggggaggg agtcgggcgc attgtgttgc cgtatggcgt gacatggccg acgttcaccg
     3421 cctatccgag caatccgatc acgatcgaat atgtctgcgg ctggactgcg gcggctttag
     3481 ttcccgaggc gattcgggca gcatgcaagc tcgtttgtgc ggacctctgg accaaccggg
     3541 aagggcaatc ggtaggatca gctcagattt actttgagaa cccggtggtg caacggctcc
     3601 tttacagcca tagactctgg gatgaatttt gatggtaatt tataagatcg aaaacaaaat
     3661 aaaggtattt cgctactaag aaagcggagg cggcatgagt gcggggattt tgaacaaaag
     3721 aataatttta gaagccccgg tcaaaacttc ggacggaatg gggggattct cagaatcgta
     3781 tagcacagtt gcagaagttt acgccgccat ctggcccgta tcggccacgg acatcctgcg
     3841 gaataatgct ccggtgatgg aggttacaca tagaatcaga atccgatatc ggagcgtgat
     3901 gaagagcgca tggcgcatct catgggcagg acggtatttc agcatcgtct ctattattga
     3961 cccgaacatg aggcatgagt ttcttgattt gctctgtaaa gaggcgacgg gatgaaaaat
     4021 ctttcaacag ccatctacac ccaattcggc acttctacgt tgtcgaccta tatcggcggg
     4081 cggctctata agggcatggc tccggatggg gtggagttcc cgtatgccgt ctattcgttg
     4141 gtttcggacg tgccggataa cgtctttgca aagcaggggg aggaggcgat catccagttt
     4201 gacttgttct ctatcctgtc aagctcaacg caagtcgaga cgatgtatga ttatttgaaa
     4261 acggcttatg atgattgctc cctgaccatc acgagcacga cgctgatctg gttccggcgg
     4321 gataatgcga tgctcatggt ggaggactac acgaccccgg cagggacgca gaaggtgtgg
     4381 cattacgccg tggattatac ggtgatctta gtagcaagtt gagaaagggg aatggaatgg
     4441 gctgttgcgg tggacaaccg gaggagaaaa ggcctccgat gaaaaacaag aacatcatcg
     4501 tcgttcagag ccttatccgg gaacttggag aggaaagcct gcgcaacccg gatgtggtgg
     4561 cggacctcat tcgagccttt gggatcgttc aatgggggcc tgacgtattt ggggccgacg
     4621 aggtgttcaa gaacccggcg gaatcaatgg ccgggattta tcagactcca tgccagcttg
     4681 ctcgtgcatt ggtattttta agcgaattaa agataggcag ttacctcgaa gtcggggtgt
     4741 ttcaaggtgg gtgcttcctg tttgtctcgg agtacttgcg gcggttcaat ccgaagatcg
     4801 tttgcctcgg gattgacccg acgaattacc tgaaccccga agtcagggaa atcatcgacg
     4861 tcgccgattg gatgaagcac gccagcatta ccagcgacca catagccgga cggaagcacg
     4921 acctcgtttt catcgacggg gatcatggga acgggtggcc tgccagagat tggaacaacg
     4981 tcggcaagca cgcgaaagtc tgcatgttgc acgatattca agggccgtcc tgcccggacg
     5041 tggttgctct ctgggaggag ctgaaaaagg acaagaaaag gtcgtgggtc gagtttcttg
     5101 aacacaacgc ccctgccccg ttgcagggaa tcggcatcat ccatgaaagg gggaaagcgt
     5161 gaaagagatt catttggtta tgcctttctc tcgcccggag aacaagggga agatcatgga
     5221 tgcgtatcgc ggactgaatg tgatcctcca tccgatcatg ttcgcagacg agactattga
     5281 gttcggggag ccgtggataa ggccttatgt catagacgag ccgtcgaagc tctgtaccgt
     5341 attgatgccg ggaacctata agcggaaccg ctggatagag gcgcatgaat tggagccgga
     5401 ggactattat cttaccgtgg atgacgatga cttctacgag cccggcgcct tcgaggaagt
     5461 ccggaaggag gacgccgata tagtcattat ctcgatgaaa cggggggtcc aggttccgga
     5521 agtggcatcc cccgaaaggg cgtacccgac ttcgacactc gttgcaagcc cggagaatat
     5581 gaggatcggg agaatcagcg cccagcagat gtttgtcaaa ggtaagttat tcaagacgca
     5641 tccccataac gaggaatctc attgttggga cggggagctg gcggaacact atcaggcctc
     5701 gcataagaat accgtgtttt tgtcacaggt ttacgctctg ttcaattatt ttgagccggg
     5761 caggtggaca ccggggctga aagtgtcgtt cggcgtaatg acgaatttgc cctcccggct
     5821 gtccatgtcc ttgcagcagt cggaaattgt cggtgacctc cattatgtca gcaacccgaa
     5881 gtcggccaca aaagggctga acaagttgct ggaccggatc gccgttgacg gctcggatgt
     5941 ggccatcctg acgcaccacg acatgagctt tcgcaagccg tggttgccaa agatcaaaaa
     6001 tgagttggcc gcccttcccc cggattgggt ggtcgccgga atcatcggga aggacatgga
     6061 aggaaggata tgcggaaaga tgagcgacag caggattccg gttttgttta atacttccga
     6121 catccacgaa tttccggtaa aggcgtgctg cttcgatgaa tgcctgatat tcgttaacat
     6181 gaagtccggc ttcaggtttg acgaggcact cgacgggttc gacctttacg gaacgctttg
     6241 cgtgttgcag acatgggaga tgggcgggtc agcttggatc attgattccg gggcatatag
     6301 cataatggcg gatactgaac acggcaacat gcaagtggat gtggctctgg ccacgcatca
     6361 ttgcacgcga ccgtttacat ggttcccgga caataaattt cagaagaatt ttaagtggct
     6421 ttatgaccgt ttcacaactg cggaacggct ggatacgacg gtgcttggct tgccgccgga
     6481 agataagagg tttgaaacat cggcggcata aaccgccata acaggaggtg tcacaatggc
     6541 taaagtatgc ggaaaaggcg ggaaggtgat gtatggcagc gtggtcgtgg ccaacatcaa
     6601 ggaatggtcc atgtcggggt tctccgtgga gacggtaaag aaagacccgg ccttcgggga
     6661 taccgtcatt gagtattgca caacggggct ttcggagccg ggaacgattt cattcaccgg
     6721 gaactatgat ccggcggaca ccaacgggca gatcgcgctt gcagccgtcg ctgcggcagg
     6781 aacggccctg acgaacctgt atctctacgc gaacacaagc acgttctggc gcgtgggttc
     6841 gggcggatcg atccttgtta ccaaggccaa tgcaatcagc ctcggacgca ccggtatcgg
     6901 gacgatcagt ttcgaggggc tggtccaggg agcaaacatg gaacaggtcg gaaccggatc
     6961 gtaagccgaa aggcggaaag gatgaatcat gctgatagat ttaagcaggg atcaggggga
     7021 gtggtttccg ttccaacagt cgcatattga ccccataacg ggggagccta tctttgaccc
     7081 cccggcgtca gaggcagcgg tgcaagttcg tagcctcgcg ccattctatg aggctcgaat
     7141 agcaaagcgg gaacggaagg tggagcatgt ctataatccc aagacccgcg caatggagcg
     7201 gatatcatat attgaagagt tatcgccagc cgctgcacgg gccgaagctg aagatgcctt
     7261 tgactatgcg atcaccgggc tcaaagggtt tgaggatagt agaacaggcg aagaattgaa
     7321 atgtgaccgc gcaacgaaga tcgcattaat gcaagttcct gtttttgata ggttcattgc
     7381 aaggtgcttc aagctcctcg cggcatcagg cataaaggag agggaagaca ccgaaaaaaa
     7441 ctgatcgacg gggtagagtg gctggatacc atcggcccgg aatgccccgg ttgccgtaag
     7501 atacatgcag aaaggaagcc gccgactacg cctccatgcg aatcctgtcg agtcgagcta
     7561 aaggtgacga acgaagcagc agcccggata tatcgcatgg tccgggggca ggtgatcaca
     7621 aggcacaacg ggaaatatga cgtgatcgtg gacttgaacc atcttgcggt atgggcggcg
     7681 atagatgctt atggcgtcca gaaccgcacg gaatgtttcg agaaggtgct tcggctgttc
     7741 taccactttc ggagtcaagc cgatgataac acaggttgaa attgtctgga tcatagcgac
     7801 tgtgctggtg gcagtagtcg gggctgttgg tttgtgcctg ttgctctggg tgatggaatg
     7861 agctgcaaga tcacttacga tttcaaagac ctcggcgtca tagaggagag aatcgaaaag
     7921 aatctcatgg accgccttga agccgcaggg gagcttatcg caacgagggc gcggcagctc
     7981 tgccctgttg ggaccacccg aaggccgcct tacaagaacg ggcagccgtg gactgcaagg
     8041 gagccgggga ccttgcgggg gtcgatccgc gtcacccgtt taaagggtga tccgaacctt
     8101 gatgtcagag tctatgccgg ggcgaggggc aagaccaatg agggctatta tgcccatatg
     8161 gtggagtacg gcagcattca caacgagaag aaatcttttc ttcgcccggc tgctaaagag
     8221 atcggaaaga acattgaaac catcatgaca tccggcggtg atgtgaccgg cggtatggag
     8281 taagcaatgg cggcaaacgc tggcaagata tttgtccaag tcgaattgga cgacaagaaa
     8341 ttcaagactt cccttgccaa tatgccttcc gatgcgaagg ctacggcaag cgggattgag
     8401 aaggtctgga aaaccctcgg cacgcagtcc gaagcgtcct tcaacgccca gaggacagcc
     8461 tacaagaacg ccctaaccct tatcaagaac gatgtcaatt cctcaaaggc cgacattgtt
     8521 agggcagaag aggcggccgc cgccaagatc aaggccatcg acacccagca atacggggaa
     8581 cgaaaatctg caataaccaa cctcaaggag cattggcttg cagcaacagc cgcagtcgcc
     8641 ggggcgatag cccttgcgaa taaagcatgg agttatgcag aagccgcctc ccaatatcag
     8701 cagtcgtcaa gtgcgtttcg ctcaatggct cagtcgatgg ggaaggatgc cacaaccgaa
     8761 ttcaacaaga taaaagtggc atcggcgggc atgatagacg acaaagccct gacggaatcg
     8821 gcaaacaagg cgatgtctct cggtattcct attgaatccc tgtcaagtct catggagatc
     8881 gcccgcgcaa agtcccgcga catgggaacg acgacgcagc aggcattcaa tgacatcgca
     8941 accggcgtcg ggagagcatc cccgttgatt cttgacaacc tcgggttatc gctgaagctc
     9001 ggcgcagcca acgaagcaat ggcggcaaaa ctcgggaaaa ccgtagcgga attaacggat
     9061 caggaaaaga aaaccgctat cttgaatgct accatcgccg ccgggtctga agcgttgact
     9121 cgttatgacc tatcccagac aaccacagcg gaaaagatgc agcagttgac gacgacgatt
     9181 gcgaatctca agctggatct cggtgcggga atcatacggg ccgccgccgg tgcttacggc
     9241 gccctgcaaa cccttgcaac cggggcgctg gttgccgccg cggggtttta taaactccaa
     9301 caggcatccc taacggttca atcatggaca tccttcggag atgcggcgaa ggcctacgca
     9361 gcggcggcaa aggaggccgg ggagaacgca aaggctgcga tgggggcaag cgaggagctt
     9421 gcagggaaag cactcgacag ctttactgta atgactgcct cagcggatga ggtatccgtt
     9481 gccacaaggg gtatcgggac cgcctccacc gaagcggctg gcgtggacga aaaggcgaag
     9541 aaaaagcaac tggaagccgc ccagaagctc gaagagcaga tccgggaaac gaaccggaag
     9601 attgccctat ccgccataga gtatgcggcg caagaggatg aggaacggga aaagtatctc
     9661 aaacaatatg aggaggccga taagaaggcc gccgaagatg aaattaagcg atggcagaag
     9721 gcatccgaag ccgccattga tgcgatgaaa gaagaggcgg aagagggggc taaacaatca
     9781 aaagccacca ttgaaaggat ggccaaggag gagaaggccg ccctcgacct tgccgacaaa
     9841 cagcttaatg ctgcgaagtc catgtattca gacatggatg attacagcga agagtattat
     9901 gaaaccgtca aggaactgct caagaaggaa gctgacgcca ggttgaagat cctcctgcag
     9961 ggtgtgaccg atacggaaga agccgatgcg ctgagaaagg caagcgccgc gaagctgaca
    10021 agcgaccttg aagcggagga gatcaagcgg ctcaaagcat caaaagactg gaaggacgga
    10081 gtcacggctt atttcaagga agcggagaaa gactgcaaga ctactgccga gcatatgtat
    10141 gacttcatga aaaccttcgc cgacgcctcc aagaccgcca tatccgatac tctatttgat
    10201 gcgatcaaag acggcacggt ggatcttgaa gatatttggg aaacgtggtc ggatagcctg
    10261 cttaaaaagt tcacggatac cgtcgcagaa atggcaacgg acttcgccgt agacggcata
    10321 aaggcgatgg tatcagcggc aaaggagccg atcagcatgg tcttttcggc ggcgtgggac
    10381 gcggcaagcg caatcgtcct tgccggaatc aaggcgcttt ctaagtggtt caacatggct
    10441 tattcgggcg gctatgtccg ggacagcggg atcggggctt atgccgatgg cggcacggtt
    10501 cccggttacg caacaggtgg cgactccccg gcaaacgata cggtccttgc tttactaagt
    10561 cccggcgaat acgtcatgcc ccggtccgca gtcaatgccg aaaccctgcc gcatctcgaa
    10621 tacatgaggg acaacaagcg gcccagggga tatgcttatg gcggcccggt agatgcgttg
    10681 ccgggactga gcaagctcta tggcgacaga tacgggcagg ctggtgtttg gtatgatgag
    10741 gacgagtggg gagccctggc ggatgcctat aaaaatctcc atgactacat cggcatgtct
    10801 tactataacg gcaagtgggg attcatcaag gagggttggg atgattcgtt cggagcatat
    10861 ccaaaggaat tttcaacagt agccgacccg gtatcataca caggagattg gttcaacagg
    10921 aacaaatacg ggagcagttt gcagtccgtg gatggagggg cgcttggtta tgatgactat
    10981 aagtatttgc tggactggat aataggatcg gtacacggaa tagcttatgg atatggagag
    11041 ggaggggaac ccgacccgta tgaagggcta cgaaacgcgc tgacaggctc acttgatccg
    11101 aacgtcgtct gggtcgagcc ggaaggctca ggctatcgct ggtatatgcg tgacggatct
    11161 gaaaaataca cagagccatc gaataatttc tttgatcgct ttatgccttc catcatcaag
    11221 gcggtcgggg cggtggccgc acttgtaatt gcaacagccg ccgcactccc aacgggtgga
    11281 caaagctaca cactattatc cggtattgtt gcagcgatgg agggcggcat ggcatccgca
    11341 ccggctttga ttggagcagc aggcacaggg ctttattccg ggcttgtgac ttacggcgaa
    11401 actgggagcg taaccgggtc aatcattgcg gggatcattg cagccgcagc aacggccctt
    11461 tccttcggca gtccgtgggg gcagtcggaa accgggcttt atgttctcaa atcagagctt
    11521 aatgccagcg gctatttgac ttctgcacag gggcgaatca ttgccgatgt gatccgaaag
    11581 ggcgcgggaa tcgtccttga ggatacgctc aacagcgtcg tcggcggcgg tggaagtatg
    11641 gcaatcagcg gcaagggcga tccgggcggc ctggacaccc ttgcaggtct ttttgaaaac
    11701 cttcctttca acaaggaagt ctcccttgcc tccggcatcg actacgtccc ctatgacaac
    11761 ttcccggcga agctccacga gggcgaggcg gtgctgacga aagaggagaa caagaaaggc
    11821 cggggcatca cggtcaattt caacttgacc ggatcggtca tcgacaagca ggcggtgaac
    11881 gagtttgcga aggcaatata tcccagactg aaacgattgg aggcttacgg ctactgatgg
    11941 gaaaggcacg gtttatttat cagaatcata tccccgatga ggatgcgttc accgtttcat
    12001 cattgcaacc cgggcaggtc acggcggcgc tcaaggacgg cagcgggtcg gcctccataa
    12061 cggtttccgg cagttttacc ggggcggtag ataaggaata tgtaattgag atcgactcca
    12121 tagcaggcgg tgcggaagtc ggacaggcta ccttcaaatg gtcggacggc ggcgggtcat
    12181 ggaatgcatc agcagttacc accccatcgg ctccgacgga gctgaacagc ggcgtgaagg
    12241 ttgccttctc atccggtgat ggtgcggatt tcgttgtcgg cgaccgttgg tatttcaagg
    12301 gcatcaatct cttcaacgct ggtaagttga ttgacctgaa tagggacagc cgataccgca
    12361 gcggatcgct tgaggagccg aacacaatcg atgccgacat gggatcggat cagagcgtgc
    12421 tcgtggtggt gatattcgat cacaatttca catccggtgc ggcgataagc atttccggcg
    12481 acagcgcaag caccttcgat tccgggatag gcggggcgcc ggaatacagc gaatcgattg
    12541 tctggaacga ggacggcatt gtgcattata tgacaacgcc ttccacatat cgctactggc
    12601 ggctgtccgt tgaggatacc tcgaattcag acggatatat cgagatcggg gagctgctga
    12661 tcggaagtta tgcagagctg tcccgcaatt acaattatga gttttcgagg tcacagggat
    12721 ttatcttcac acaaaacgag acggcgatgg gcatccgcag agcccggtat tacaatgcac
    12781 gccggattat agaaatcaac cagacatatt tgacgcttag cgatatttct aaacttgaaa
    12841 atatggcaga aacgctgaac agcaaatcag ccggaaccat gaaaccgttt tatttcaatt
    12901 tcgacagcgc ggttccgaac gataccttca tggtgcaact gacagggctt gcccgtgaaa
    12961 gggcggggac tgcggccgag ctgtatcaca gtccgatgac tttggaagag gttatcaaga
    13021 gtgattagaa taccgattgc ctttcacaag aatctactgc gcggggagat accaatcgcc
    13081 tatgtgatga ttataacgca tctggcgaac cggatctatt ccgaccgcga gttgaaaggc
    13141 atctttgccg ctgctggctg ccttgcggac ggaacctatc ttgctgacgg ttcaatcctt
    13201 gccggggcgc agagcacggg cggcgttgag aagagtgcgc gggttcttga tttcggcaag
    13261 ttcgagcgga gcatcgtccc gcagaccagc gacctactgc ttgcctatac cgggaagcag
    13321 attcagaaca accaagtcgt gcttgataac acggataatt atttcacgag gatgatcgca
    13381 caggaaccat ttctgacaag ggagcttcgc atcctgatcg ggttcgagga ccagccacag
    13441 gacgatcatt ttccgctatt tgccgggatt atcacggaca ttgaagtcga tgagaactca
    13501 atgaccatcg aggcagggga aatgtaacat ggcttatacg cttgaaaatg gcaatatcgt
    13561 aaaagcatgg gcatcatctt gctatggttc agcgcatatg ccgctgaatg ccttttatac
    13621 aggcacaccc ctgaccgggg gaagcaattg gcgatcttat gctacccctg tcggcatcac
    13681 atcatattct ctcacatgcc aatttaattc tgcgaaagtc atcacttatg tctggtattc
    13741 caactttttt gactgctatg atgacgatac gacatattat cccggctcta acacatcagg
    13801 atctgctgga ttcacactat acggatcgaa tgataactct aattggactg aaattacttc
    13861 cggaacattc gcaatccatc ctgcggctaa tgttgcatct ggcggtgagt tcgctttttc
    13921 taacagcacc gcatacacct attatttatt ctatgtatct gcctctcatg gcggtcattt
    13981 ggctgccatc cgcagattgc agctttattc tggcagcacc actcccccga caagcgttcc
    14041 aacagtaaca accacttcca tatcgggcat tctggcaacc ggggcaacag gtggtgggaa
    14101 tgtgaccagc ggtggcggtg cgacggtaac ggcaaggggc gtttgctgga atgatacagg
    14161 ctcccctacg acggcagaca gcaagacaac cgacggaacc gggaccgggc tatttgtatc
    14221 gactctttcc ggcctgacaa aagggaagaa atactatgtc cgggcttatg ctaccaactc
    14281 acaagggacc ggatatggaa gccaggtcga gttcacgacg cttgacgttc ctaaggtaac
    14341 gacagcggcg gcaacggaca tcaccggaat tagcgcgaag gtttccggcg cgataaccga
    14401 tacgggcggc gaaaatgcct ctaccatcgg tatctgctgg aatacgggag ggacgccgac
    14461 aactgctgac agcaagtata gcaagacttc atataaggca tacggcgtcg gaacattctc
    14521 gggagcgcct tatactgcaa gcccgctcct ccccaaaaca aagtattacg tccgcgccta
    14581 tgccatcaac tccgtcggaa ccggatacgg caacgagatc gaattaacca cgcttgcatt
    14641 cccgaccgtc actaccacgg ccatatccag cattgctggc acaactgcat ccagcggagg
    14701 aaacgtaacg aatgagggcg atgaatccgt aacagctcgg ggggtatgct ggaacacaac
    14761 gggatcgccg acgacagcgg atagcaagac cagcgacggt tcgggttcgg gggcattcgt
    14821 atcaagcatc accgggctga cacggggcgt gacttattat gtccgggcat atgcgacgag
    14881 cagcgtcggc acggcttacg gcgaagaggt atccttcacc accctgaccg ttccgacgat
    14941 ggcgaccaaa agtgttaaga acatcacgaa ggcaaccgcc cttgtcgaat gtgaaatcgt
    15001 ggccatcccc ggtgccgcga aatgcacgac gcgaggcatc tgttggagca cttcggccaa
    15061 tcctaccacc gcagacagta aggcttttga caacggaggc gggacgtatg atgtcggcgg
    15121 gtataccaag acggcttctg ggttaagccc tgtcacgcgg tattatgtcc gggcatacgc
    15181 gatcaactca gagggaacgg cttacggggc acaactgtca tttgtcacgc tctccaaagc
    15241 cgcaaccatc gcgcctcaat ataagctcac ggacacctat tatctcggtc gcacgggacg
    15301 ttattctctg cctcaaaaca gcaacgaccg cctgccgctt gtctatggcg acctgacaga
    15361 ctcctccgaa ggcaactgga agctgccgtg catcgacacg aatacttatg tctattgctt
    15421 cgcggcgcat gaggttttgt ccgtggccaa cgggaacagc atcacaatct atgaaaacgg
    15481 cgttgaggtg gatagcggcg attatgcctt cgatgaatcg aatgactacg agggcgaagg
    15541 cgctattgca accgtaacgt tttcaacgcc caaaaccgga tcaaccatca cggcgcgggg
    15601 catggggaag ccgacgacat ccggcggggc aacgctcatg gaaaacatca tcgacatcct
    15661 tgaagatgtc ctgacggtcg aacttgatat ggatattgac ttcgaagaaa cctacaaggc
    15721 aacagcgcgt gacgactttg aagggcagag ctatgtagcc gcaggcgtga tctataagga
    15781 tatcaaaata tgggatttga tcttaatgat gatgggctcg tttttgggat ctgcttacat
    15841 gaacggagca ggggagcttg ttttggagat cgacgacggc acctatccgc cggaatcgtt
    15901 ttcgggaatt gtcagtaaat cggacgcggt ttttaggtct gcctcgatga gcctcagtaa
    15961 tcttatcaac cgctgtcctg cgtcctatgc ctatgactat gcaacaggca atgatttcaa
    16021 gaatcattcg gatggcgtaa caaccgcatc atccgcctct gagcacattt acggcgtccg
    16081 ggaaccgacg ctgccttata agttttactg gtgccgggat gcagcatcgg ttgcggtgat
    16141 gcagcagatc atcgtggata agttcgcata cccactctgg accatcgaat tcgacgacat
    16201 cacgatgaag cggctcgatt cggatgtcgg caagctgatc gcttacagcg tggatcggct
    16261 ctatgacaag gacatggccg cgatgctcaa cacgctatgg aaggtggtct catacaatcc
    16321 tgatatccag cgcggttcga tcggattcag agcaatcgag acgggttttt atctgaccca
    16381 tgcctatatt gcgaatggtg attggatcgc cgatggctcg ataaaggctg ggaacaacag
    16441 ggacgttacg aattacggtt aggaggaaaa tgatatggcc gaccagatta tacaggcaac
    16501 agaggagatg gtgggggcgg ggcatgcgac aaaagccgat acgctgaatc gcgcctttct
    16561 catagaacac aataccgacg ggacgcatga gaagctctcc gtcggctccg acgcgaatgg
    16621 cgatatgtat tacagggcaa attccgtgct ggcgaggctg gcgaagggaa cggcaaacta
    16681 caagatgttt atgaacgccg ggggaactgc accggaatgg gcgatgggga taaaaacaat
    16741 ctcttcgtcg tatgacctct caacggaatc cggagaccaa tcaataactg gtgtaggttt
    16801 taggccatgt gcatatatct ttttggggac tatttcggca gatgtggtat ggaatatagg
    16861 attctatgca cttgcggcat actcgatcca atttcgggga gatacatcga aaatgctttc
    16921 agggccggga tatatttcgc gtatatacct atcatcaggg agtgtcaata ccgtgccaaa
    16981 atctatcgaa tcagatggta tcacgattac tcatacaaag agcggcagtc ccaccggaac
    17041 attggtccaa tactggatgt tctttagata ggaggaatta tgagaatctg catacggaaa
    17101 tcagataagc ggatcattga gatgcagtcg aatgcgacag aaggaacatt gatcaccaat
    17161 gccctcaccg caggatatac attggatgaa attgaggagc gcgaggtcga tgaggccggg
    17221 tatgctgacg cccttgcaga agatccggta gaaatcgcgg caaaggcaaa ggcagaagca
    17281 gatgcgagtg cacaggctgc gaagacggta gcgatagcgg atgcgaagga ttccatctct
    17341 accattgaaa cggcggtaga aaaggccgcc gatctcgaaa cgctccgcaa gaacgtgctg
    17401 gagctggtca aggcggtgaa ggttctgacg gcgtaaacgt gtccagatgt gtcctggaat
    17461 gccacaaaaa ggggctgcgg aatcactcgc aaccccttga tttatattat caatcaggca
    17521 gtcatgcctt atcacgtcgt attttcagtc ataatgggac aaccagccta tctatttgat
    17581 ttaattacct gtaatttcgg ggggtgtgtc caaagtgagc gtttgcgcgg aaatgcctaa
    17641 attttccgca aaagcacatt gtttcatgcc tattttcttc cgtatctctt tgatttcctt
    17701 gccgtttaaa aatttcttca ttttagatta aaatttttct tgactttctt aatctgagcg
    17761 tttataagcc taatcaacga agttaatcca caacacaaac gaggttaatc aatgggcact
    17821 aaagcaaact cccgacgaaa attcaaccga aatcttttct acggcaaact ccataccgat
    17881 ggtctgaccc ttgaaaaatt ggggcaacag ctcaacccgc ctatcagccg attccgtgct
    17941 tgccagattg tgaacaaggg caaaccggcg caccggcttc aggaaatcgc atcgatcctc
    18001 aaaaccaacg tccagactct tttcccgaag gaggtcggca atgccgaatg atgccatgtc
    18061 tgacatcgac aaactggaaa acatattcag cctccgtatc ccatccgcaa cgaaaagaat
    18121 gctcgacgat ctaaccaccg tcgaaaaagc caagctcaat gaagccgtca gaaacaccat
    18181 agccagagcc atccatgaat cgaagtttga cccgcgactc taccttagag acgcgagtga
    18241 atgaagtcaa ggtaaacacg gttctacttc gtagggggat gtaaacatga aaatgatacc
    18301 gctaaccaac ggacaaaaag cgatagttga tgatgaagat tttgataaag tatcagcttt
    18361 taattggttt ttggtacacg agagaaacgg aacgaaacga gcaaggagaa ccactccaga
    18421 ctacatagat atggcacgta tagttcttga agtgccgggt gggtttatag ctgaccacat
    18481 aaacgggaat cctctcgaca atcgcaaggc aaatctccgc ctcgcctcaa aacagcagaa
    18541 tctaaggaac cgaggaaagg gcttatggct tggacgatca tcgtctcgat acaagggggt
    18601 atgttttctt aggggaaagt ggcaggcaca aataagtaaa gaccagagag ccatttatct
    18661 cggaacattc ccttgtcagt tttgcgccgc cctcgcctat gacatcgcag ccgtcgaaca
    18721 cttcggggaa tttgcaagac ctaatttctt gaaggctcca aaagggagtt cagccgcatg
    18781 actaaactcg aacgtctcga aactcgcata gaatctttag aaacccgcct cgacgacctc
    18841 ttggggcgtc ctcgggggcc gatcagcctt acggaatacc ggatcgcctg cgagacgggc
    18901 gaccgggaga cgatgaggcg gtttttgaag caagaggaaa aggtgggttt ggccgtaaca
    18961 gcctccgaaa acggcggtga cactatatcg ggcggggtga ctcactcggt ccgccccgcc
    19021 caacattata ggaggcagcc atgactgaac tcgcattgtg tctaatcctt atcagcgttc
    19081 ttgtcctcgc cgtctgcatc ctccacgcgg cgggtgtgat gttggttaga tggcggtcat
    19141 acaggacggg caagcgggaa tacaggatag tccagagggt gctgtgaaga aacatcccta
    19201 caaatgcgtt tactgcggac ggaccatcgg cgcacccatc gggcataggt gcaggggaac
    19261 catgaggtat cgccgcctgc gattcataaa agaggacggc tcaatttggg gaagaggagg
    19321 gcactatggc accacaggca aggacaacgc aagaccagct tgaatgggat cagtttaaaa
    19381 gtcagatcga aaccatgttg aaagcgttcg ggacgctgct atttctcccg ctgattcttc
    19441 tcgcggcaat ggcggttggc ctccgtgaag gcatcgtggc gggggcgaaa gcaagtctga
    19501 gcgcaatcga gaggtggtgc tgagatgagc acgaagcatt tcgagagtgc gcgtgatgca
    19561 gagattaagc ggcttaaaac ggagcttgcc aaatacgaca cgcctcgtga cgagacaacc
    19621 ggattgcgga tcaagatagt ggagctgcaa aaggagcttg ccgagctgaa agcggagatt
    19681 gaacggctga agaaaccaaa ggggttccat gacgtgagcg acctttatga tgatggcatt
    19741 acgggcagac agtggatgat agagcaggag gcgtcccatg aatgacaccc gccgcaagat
    19801 gctcaccgag tatctcggaa gctgctggca tgagatagtc gataaaggcc catacctttc
    19861 aacgtgtagt aagtgcggaa tgacctttgg cgcaatccat acgagtgatt gggaccccgc
    19921 aggtttcaac cgcaccttcg ccacccatgc cgacctgcat gcgcttgtgt gcaagatgaa
    19981 ggagaaacag gagctctggg agtttgagca aatatttgct ttaaaaagat ggcatgacta
    20041 cacctcgaac ggagaaacat atctctcttg gctcatcacc gacccagccc ggacctgtga
    20101 gctgatcgcg gagtggatgg aggcgaagca tggacacgat aacaaagacg cctgataaca
    20161 gtacaatgcg cacagaaagc ctatcccctg ttgcatacag gctacggaaa gacagggtag
    20221 atggccttgt cttacaaggc tgctactcat ggacggagga atcagataat cctttcggaa
    20281 taatacatgg acatgagtgg cgggacatcc ctacggtgag agaataggag gacaagcatg
    20341 actgacctac tctggtttct tctcggcctt tttgtcggtg cgaacgtggg gtttcttgcg
    20401 ttctcgttat tcgctgcgag ggagcgatga aggtccgcac ccgctttctt atttaccgga
    20461 tgcttcgccg cttatgcatc ccgcacagga tcgcatatcg gcttgtgagt cgggcaaaca
    20521 agacaagcaa agagcctatc gtcgaaagcg gatgggcgag gagggggaga tgaaattata
    20581 ccgaattaaa ttgagaggga tgtgcggtgg tttgacgacg caagtggcat acggatgccc
    20641 ttatgttgtt gctgagagtc ctaacaaggc attggagatt gttcaaagtt atttaagtaa
    20701 aaaagacatc gggttttccg atgaacgcga gatggtaaac atcgaactac tggcagagca
    20761 agcggattat ccatcttgca agattcagtt gttcatacaa gggatagagg cccaatgacc
    20821 ttcaccgaag ccgacaagtt gccggaaatc ccggtgctga aacgaataga aaggagggtg
    20881 tgcaatgtgg gtgcgaattg tcaaggtggc ggtagcgtgg atgctgagaa agcacaagtg
    20941 gatgatttac gaaatgattg tgccggacgg ataccatgtc cacaaaaatc ccgaaaaggt
    21001 gaaggcatga gcgcaatcga cgacatccgc gagagggtcc gcatgggcct ccatctggag
    21061 aatgccgacg cggagctgct tctggatgag cttgacgcgt cgaatatccg gtgtctgcaa
    21121 tactcgcggc ttgtaggcga aacacgcggg aaaatacagg ccatgattat ggagatggag
    21181 agggggcaag aggccagaat cagggatctg gcggcaagaa ggacggcggc caaagaagcg
    21241 atagaaaggg ggggagtatg aacgaattac agaagtatcg gaggccgttg acagcgattg
    21301 acatcaaggc ccaggtgcaa ctcattcagg aggtaatgaa ggcggtcatg gagaaggatg
    21361 tccattacgg gatcatcccc ggaacgccga aaccgacgct ctacaagccg ggatcggaaa
    21421 agctattgtc cacttttcgg atcggtgtag agccggaaac catcgacctt tcaacacccg
    21481 atgaggtccg ctaccaagtg aaggcgaggg ggttttccca ggaaacgggc gccttgctcg
    21541 gggtggggat cggggaatgc tcatcgaatg aggaaaaata caaatggcgc cgcccggtct
    21601 gtgatgagga attcaacgaa accccggagg accggaagcg ggttgtctgg aagcggggcc
    21661 agcagaagaa ctaccagcag aaacaaatca ggacgaaccc ggcggacatg gccaatacca
    21721 tcctcaagat ggccaagaag cgggcgcaga tcgaccttac tctgaccgtc acggcggcct
    21781 cggacatctt cgaccaggac cttgaggatc ttcccgccgg agtcgtggag gggatagtcg
    21841 aaaaggaatc ggagaagaag ccccccgtcg ccacacccca aaagaaagcc aaagacccga
    21901 acgaaccggc gacggagccg caaatcaaag cgatccatgc cctgctcggg aagctgggga
    21961 tcaaagacga ttttgaaaag cacatgaggg tgtccaggat tatcggtatc ccagagcccg
    22021 aagtgatcac gtccatgtca aaactcacaa agggccaggc cagcactgtc attgaaacgc
    22081 tgacgaaaga gggaagcaat gaaggcgatt ttggacatta agagcggcca tgtagagccg
    22141 tggacatctc tccaactggc cgcttatacc ctgctcgacg ctccggttga gtttgaatca
    22201 gacggccacc gatacgggaa tggccttgag tccgtcacgc agattttgaa ggccgagcag
    22261 ctcatcgaca cccgcttcta tgacgattgg agccgggacc gggggagcat gatccacctg
    22321 gccactcatt acgacgacat gggggagctg gacgaggaaa cccttgatcc ggttatccgg
    22381 ccctaccttg aggcatggcg gaaattcagg aaggagagcg ggtttgtccc ggaagtgatc
    22441 gaggttccca tgatgtcaat caactatcgc tatgccggca cgccggacag gatcgggcag
    22501 ctgcccggca agatcaagag ggcagcggta gagctacatg acgatggcgg gtatcgattg
    22561 ataccataca cggacaggca ggatgtccat atttggctga gcgtccttgc cgtccataac
    22621 tggaaacgca acaacctgaa aggaaggtga gaacatgatg gatgattttc agttactgca
    22681 atttattcag gaaacgaaag agcgcattct cgcccttgaa gcgcgagtgc atgcccttga
    22741 atcatggcgt gatgaagatg aaacatacaa aatggaacag aaggagcgcg aatgaaattt
    22801 aatagcaagg ccgaagccgt aaggtttgct aaggccgaaa ctaaccagac acaccgaaaa
    22861 cacgaagctg tcccggcacg gtggcaaggg gtggatggta aatggcatga ctgttggaca
    22921 gttatcctga taacgatagg aaggtgaaca tgggagaggc agcattgagt ttagtcgaga
    22981 ccacacccgt tgaggaaaag gctttgtcca tcgtggacca ggcaaaggcc gtcaaggtga
    23041 cggatgcaca gacctacacc gccgccggca gcctctggaa gtccatcggg gagatgatca
    23101 aggaagtcaa ggatacattc gatcccatct gtgacgcggc tcataaggcc cacaagcagg
    23161 ccgtcgagaa acgggcgaaa taccttgatc ccctgacagc cgcgcagaag tccgtcaagg
    23221 ggctgatgtc cgcctatgac caggaacagg agtgcctacg ccgtgaagaa caggcacgcc
    23281 ttgaagccat tgcccggaaa gcagaagagg atagacggct gcaagaggcc atcgaagcgg
    23341 aagcggcaat gaaggcacag ggagccagcc aggaggaagt cgcccaggaa accgccgcga
    23401 tcctcgaaac gcccgttagt gtcgccccgg tcgtgctgcc aaaggccacg ccgaaactgc
    23461 aaggggggcc ggtttacaga acggtatgga aggcgcgggt catcaacgaa aggctgatcc
    23521 ctcgggaata tctcgttccc gatatggtga agatcaacgg cgtggtgcgg tcccttaaag
    23581 gggcggcaaa tatccctggc gttcaagctt acgaagagag gtgctgaaat gatcggaccc
    23641 aaaaccatcg aatctatgaa gtcccttgcg gcggagaaga tcgacactta cgccgagaag
    23701 atgaatgaat ctttcctcaa atccgatgac ggcaagcttc gggttacatt ggcatttgaa
    23761 ctgtccatgt cggagaagcc ggattccgtc gatctggatt gcacaatcag ttttaccagc
    23821 gagaaaatca aggacaaaac cagcacgacg atcacggaag ctcaggcgga gctgccgctt
    23881 gcggataagg tctacaggtt ggggaaatga tgggcatgac aagtttattt aacacggatt
    23941 gcatggaagc cgtgaagaag atgcccgctg aggtttattc taaaacccag gcgctaaggc
    24001 ataaattgat taacgatcat tttcaaaact ataaaacata tcagattcca aaggctcaac
    24061 ttgtcattgc tgatattcca tacaacgtcg ggaaaaacgc gtatggctca aatcccgcct
    24121 ggtatatcga cggcgataat tcgaacgggc agagcgatct tgcgggcaca tccttttttg
    24181 ataccgacaa ggatttccga atttctgagt ttctcaattt ctgcgcaagg atgattatca
    24241 aggagccgaa ggaaaccggg aaagctccgg ccatgattat attttgcgcc ttcgatcaac
    24301 aaatggaact catcgaaaag gcgaaacagt ggggattaaa tagatatatc aatctcgtat
    24361 ttatcaagaa cttttctgca caagtcctga aggcgaatat gcggattgtc ggaaactgcg
    24421 aatacgcgat tgttttatat cgtgacaagt tacctaaatt caacaacggc gggaagatga
    24481 tctttaacgc tataccgtgg gaaaaggacg gaaagagcga aaaaatccac ccgaaccaaa
    24541 agcccgtcaa gctgctcaaa aagctcatca acattttcac agatccgggg gatgttgtca
    24601 ttgatcccgt cgccggaagc gggtcaacaa taatagcagc gattcagtca gggagaagtg
    24661 catatggatt cgaaattaaa aagaactttt atcgggaagc cagtaaatgg atcgagagag
    24721 aattaaaaca acaggtcttg ccgttatgaa cacaaaacaa aaggtcaaat ggtgcaggaa
    24781 catggaggat gagccctcat gtggaggaac cagaagaagc tcacaccgga aggacaacta
    24841 actgcgagcg tccggtcgct actcagggcg gcggggatct ttcattacaa gcactggggt
    24901 ggtccgatgg gctatcctgg cgttgcggac attatcggct gctacaaggg ccgaatgatt
    24961 gcaatcgagc tgaagagccc gacagggaag cccacgccgg agcaagagcg ttttatccag
    25021 aacgtgaacg atgcgggcgg gatagcgttc gtggctcgct caatagatga agtgattgag
    25081 gggttaaacc ttcaagatcg ctttttgaga tttggaagat aaaacatggg aaagcaacca
    25141 gcatttcagt tttacccagg ggactggacc cgtgatcttg atgaccagga tcttgaaacc
    25201 gagggggcat ggatcagaat tatttgtcgg ctctggtggt cggaaacaag gggtgaagcg
    25261 acaaaatcaa tcggtgaatg gtctcgcatt ttgagaaaat ctgaaaaaaa aacgatgaaa
    25321 atttttcaaa atttgatcgc aaaacgtata gcggacggat gccttttgga taaccaaaat
    25381 ataaccataa tttcaaggcg gatgaagcgt atggttgaaa taagccaaat aagataccaa
    25441 gttggcttaa agggtggaaa tcctgccctt atgaaaacgc gaaataattt ggttaaccaa
    25501 acgtccaacc aaaacgctcg ttcttcttct tctacttctt cttctataaa gaaagaaagt
    25561 ataaagaaag aaaaaatcca attcatcaac aacaaattcc aaaccatccc ggatgcgctc
    25621 attacgaaat ggcgtgaggt tgctccggga atcatcatta acgacgaaat aaagaaagca
    25681 gaactttggt tactcgctca tccggaaaag cgaaggagtc gttatgatgt attcctgtca
    25741 aactggatgg ttagggcgca ggagaggttc atcaaatacg ggggaggaaa tggcagaact
    25801 aaaccagatt tcacagggga caagcgtgaa tcggttcaga ccaagatcga cgcagacctg
    25861 gcaaaagcca acgcactcta tgctgctgcg aaagctgccg ccgcttttcg tgccggagga
    25921 gatgccggaa acgacgatgc gccggatttt caaaatgggt gaatatgagg ctatggtttc
    25981 gtatctgttg gcataccaac gcggggggga tctgcgccga tgggatgaat tcaggcacaa
    26041 gataccagac gacctgttga cggcttgggg aacggttctc atcgaatgta gacgcaacgg
    26101 gtttgtcaat ccccatgtgt tcaatggtga aatcgtatgg gaccagatcc ttgacaacgg
    26161 ctctggtgaa aggcgcaacg ctgaccgatg cacactgtgt gaacgggatt gcaaacggcg
    26221 ggagctgatt gatagcggtg aaacaaagcg gcttaggata ttaaatccag gatatcacga
    26281 gaagccgccg tgctgggatg tgaggccgag ccatgaaacg taaccttgcc ccgttcctca
    26341 tcctggcctt tgccctgtat ggattctggc ggctcatgtt tgactggttc ggttcttata
    26401 tcaggcaggc gtgcgtctat atcgacggga tgggcgcggc gttctgggtg tgcctggctg
    26461 cggcgggggt gatcgtggtg gtggtgcata ggagggttca ctaatgggcg agcaaaggag
    26521 gaggaggatg gcgggtaatg taatgccgat gatggttccg agggtaggcg gacagcagca
    26581 gatgtttgac ataaccgagg cggtgccgaa ggtctgcgac aagtgcaagg gcgaacactt
    26641 cgacaaggtc tatcgtctcg ggatgatacc gaagatggct atacgaaaca agaccggaca
    26701 ggagatccgg gttgaatacc aaactttcct ctgccgatca tgcggccatg agttcggcgt
    26761 gcccgtggtg gtgcagtgat ggcagacaag gacgggaagg tcgtgcagcc gccttataaa
    26821 tgtaattggt gcggtgagga tttgcatgat cgatatgggc gggcgatcta taacgatttt
    26881 aaagggcggc agtattgcac ggcgtattgc cgcaatgctc aattagaagc agagaagcgg
    26941 gacagggaat gcagcaatga agcctaaact atccaccatc aagccacgca tacagaccgt
    27001 ggacttacgg cgtggggctg gggtagggac ggagaggatc cggggcgggc ggctaaccaa
    27061 gatccgggag cgcatcctcc tccgggattg ctatacctgc cgggtgtgtg ggcgtgtcgg
    27121 gacggacctg gttgtcgatc acatcatccc attggctgac ggtgggcgtg aggatgacag
    27181 caaccgccag gcgttatgtg ttgcgtgcca tgatgcgaag tctgagcgcg aggagaaggg
    27241 gcggggggca tcgcattctt cacagcttta gcggcccgcg taaccgcgac cggtgctatg
    27301 cggacaatta atttccggtt ttgattttca aacacagaat ttcatggtga cgaaatggtt
    27361 gaaatctcga taaaaacggt aaaattgagc acgatcaagc tgaataaaga gaatccgcgc
    27421 cgtatttcca atcaggacat ggagcgcctc gtcaaatcct tgcaggactt ccccgaaatg
    27481 atgaaattac gggagattgt ggttgacgag accatgacgg tcctgggcgg gaatatgcga
    27541 tacctcgcct tgaagaaaat cggcgcgaag gaatgcacgg ccaagatcgt taccggcctg
    27601 accccggagc aaaagcggga gtttgtcatt aaagacaatt ctcaatttgg cgaatgggac
    27661 atggatttac tgtcagcatg ggatgacctg ccccttgtcg attggggggt agatttgccg
    27721 gatgattggt tgaatccggg tggtggtgat ggaggtcaac aaacaggaag tggaaacc
//
LOCUS       DAOSMF010000017        26577 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_2341_length_26577_cov_24.262876, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000017 DAOSMF010000000
VERSION     DAOSMF010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 26577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26577
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2341_length_26577_cov_24.262876"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="PLT63_03660"
     CDS             <1..626
                     /locus_tag="PLT63_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97852.1"
                     /translation="ELKQVVAELAQAQKRTEERVEELAQAQKRTEERVEELAQAQKRT
                     EERVEELAQAQKRTEEAVYHLSRRLDDTNRQLGGLAMTVGYTLENSAYVYLPALLKAD
                     YGVSCEEPLYRDYLTDNKGADVEVNILGRAKKDAQDLLIVGASKAQLSKKDVQAFIKR
                     ITRIDTGGLRPFPVLVTHMISQRDVKNYAQSQGVAIYLSYQFQNPVH"
     gene            complement(714..1655)
                     /locus_tag="PLT63_03665"
     CDS             complement(714..1655)
                     /locus_tag="PLT63_03665"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOD97853.1"
                     /translation="MENTDANQDLEQLERLMSPCVLCPRECRAERRGGAKGFCGLGTE
                     IVVSDAMPHYGEEPPLSGRHGAGTIFFSSCNLRCIFCQNHQISRKTTGSILTPEELAE
                     VMLRLQERGCHNIDAVTPTHQTPPIMVALDIARRRGLTIPYIHNCGGYESLEVIRLLA
                     GRVEIYMPDMKYGDDDLAQEFSGVKEYRRHARAALTEMARQVGDGLELREGVAVRGLL
                     IRHLVLPGCLENSFTVLDDIRASVSTGVSLSIMSQYTPAAQACRHPLLGRRIRRDEYR
                     RVVDYALALGFSQLFVQEVDDRHLSPDFDRDDPFNWE"
     gene            complement(1834..2937)
                     /locus_tag="PLT63_03670"
     CDS             complement(1834..2937)
                     /locus_tag="PLT63_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97854.1"
                     /translation="MNRLCVKAGPGAFRRIRDGGFTLDAVTCYAGPAVGPRWLIAGGF
                     DLTLIKEQVLGRTRPLTLIGASAGAWRFAAWIQPEAEKSYRALRKAYIETIYTRTDRP
                     TTIRESLISIIDRAIENDALPFALNSRNYRLAVITARSRRLAASKNPLLQRLGFAGAF
                     LANTVKRSWLYNFQERVVFFSGPKPPAFTLRPDFRGVYVPLNTTNFKPALVASGAIPL
                     LVAGIRDIYGAPRGVYRDGGLVDYHLAQNFGAGENDLTLLFLHQDRLIPGWLDKRLVK
                     RKPAAADLHNLILVHPAEEFVASLPGGKVPDRDDFVTFLDDPQTRIANWRRAVAAAEP
                     LGEIFLELVAGGRIRELVRPLTDNGTGDGRPKP"
     gene            3099..4355
                     /locus_tag="PLT63_03675"
     CDS             3099..4355
                     /locus_tag="PLT63_03675"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HOD97855.1"
                     /translation="MSVRTQVLTMAREAKMAAAVLSVLSAADKNRILRAVGAALRENA
                     SYLQEENARDVAAAAEKGLPAAMIDRLTLKEATIAAMAAGCDEVAALDDPVGKITSMW
                     RRPNGLLVGRMRIPLGVIGIIYESRPNVTVDAAALCLKSGNAVILRGGSEAINSNRAV
                     AGIITDVLKRGPLPPDAIQLVPMTDREAVYELLQLEEWIDLIIPRGGEDLIRTVVSHS
                     KIPVIKHYKGVCHVFVDETAELEMAVRICMNAKVQRPGVCNALETLLVHREIAPRFLP
                     LLAEQYRQAGVTLRGCPVTQEILPGVATATEADWHAEYLDLILAVKVVEDLDAAMDHI
                     RRYGSLHTEAIVTSDYGRAQRFLQEVQSSTVLVNASTRFSDGFELGLGAEIGISTTKL
                     HAYGPMGLEELTTSKFIIYGNGQVRT"
     gene            4352..5002
                     /gene="nadD"
                     /locus_tag="PLT63_03680"
     CDS             4352..5002
                     /gene="nadD"
                     /locus_tag="PLT63_03680"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HOD97856.1"
                     /translation="MKWGLLGGTFDPIHIGHLRCAEEILEIFDLNRIMFIPASRPPHK
                     IDGEITSFYHREQMVRLAIENNPSFSFSDAENRKEGKSYSVETVEFILKKYLQNLELY
                     FIVGQDAFHAITTWKDWEKLLQLCNFVVMTRPGYENKGLAGILPPEFSSRFHYEAREN
                     GFRGPTGYMIYFREVTFLDISSSRIRQRVRDGKSVTYLTTDPVRHYIINKGLYRSL"
     gene            5036..5407
                     /locus_tag="PLT63_03685"
     CDS             5036..5407
                     /locus_tag="PLT63_03685"
                     /inference="COORDINATES: protein motif:HMM:NF022459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2845 domain-containing protein"
                     /protein_id="HOD97857.1"
                     /translation="MQVLVYGALSAVLTLVFLLSSPPPGHAFRCGSGMVSVGDSKGKV
                     LIECGKPTFKEKVADRETRYASTGKSTKGKSGKTTKTVEQWTYNCGKDDFIYILTFEG
                     GKLIREDTDGRGRGESECKGR"
     gene            complement(5450..7048)
                     /locus_tag="PLT63_03690"
     CDS             complement(5450..7048)
                     /locus_tag="PLT63_03690"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HOD97858.1"
                     /translation="MLELMRKHARNWLMKLLLAIIIIVFIFYFGSMTGRQKAEAIATV
                     DGRIITYMEYEKEYRGLVDMYNRRFGGRMTEEMMKGLNLKEQAFNKLIYQAVLMKKAQ
                     DLKIMVTDDEIRTYISLIPAFQRDGAFDSRLYQQMLRYNKITPEDFENDQRKSLLIMK
                     VEDLIQDGIHVSDQEVRNFYQAQSEKINLAFIRFRGGDFAGRVQAGTTELEKYRKEHQ
                     GDFRTPEQIRIKYLLFAPEDFTGKVKVTDNEIADFYENRKQQWTKDGKTVPLAGVRDR
                     IIAEIKRAQAMKLAFEASRQARETIYQQENFEAYAAAQGLKVTETGFFSEKEPPEIFR
                     RLPDFAPTVFSLQKDELSKVISSERGYYLIKAGARKPAYTPELKEIEKEIRSRYIVAE
                     GRRRAGQEADAALARLRKGEKLADIGRDMKLKIEETGFFLPTGPVPRIGSSGQLTRAL
                     FLLSPKKPYGDEIFTIDGDHLLVELRGREKSEQELPADQKEQIRRACLHLKRNEAIQT
                     WIEASKSAMIKEGKLKINREAKEL"
     gene            7229..8266
                     /locus_tag="PLT63_03695"
     CDS             7229..8266
                     /locus_tag="PLT63_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOD97859.1"
                     /translation="MLFDFILGKFSNDLAIDLGTANTLVYVKGKGIVLSEPSVVAVHM
                     DSKGVKKVLAVGAEAKKMVGRTPGNIRAIRPMRDGVIADFDITEEMLRHFILSVHNRR
                     TLVRPRIIVSVPSGITQVEKRAVRETVESAGAREIYLIEEPMAAALGAGLPITEPTSS
                     MVVDIGGGTTEVAVISLSGIVYAKSLRVAGDKIDEEIVQYMKRKYSLLVGERTGEIIK
                     TTIGCAYPDKEIRTVEVKGRDLISGIPKIIEINSEEVREAIAEPISLIVDTVKDALEN
                     APPELAGDIVDRGIMLTGGGALLRNLDVLIREETGLPVTIADDPLSTVARGAGMALDQ
                     LDVLKEVTLQI"
     gene            8483..9310
                     /gene="mreC"
                     /locus_tag="PLT63_03700"
     CDS             8483..9310
                     /gene="mreC"
                     /locus_tag="PLT63_03700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOD97860.1"
                     /translation="MPIPKKYRFMTVIIAVVAISVVILLISAKGSYDTGLPRKIILDV
                     AAPMERILRAPLDGIAEIWSRYLFLVGTGEENRRLKNENDRLKNQLLQYEEGYLEGKR
                     LQKLLALKERSEYEALPARIIGIDQKSLLKTILINKGSAHGIRNGLPVINDQGVLGRI
                     IETSWHVSRVLLISDGNSNIDAMIQGSRVQGILQGTGAMRCTLKYIAKTEEVKEGDLV
                     VSSGMTEIFPKGLLLGTVIRAGKKAPGLFQTIEVAPAVDFTRMEEALVLVVPEVMKK"
     gene            9307..9816
                     /locus_tag="PLT63_03705"
     CDS             9307..9816
                     /locus_tag="PLT63_03705"
                     /inference="COORDINATES: protein motif:HMM:NF016019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97861.1"
                     /translation="MIFYLVVPWFLMIVVVFQHAVLGAVFFHSIAVEISLVLVIYAGL
                     RMKVLRGAILALILGLMMDCITGAGSGSYAFVYTMVYFMVYLLSVHVYVENAPFLIFL
                     TLVCGLVEGLLLLVLNRMLYGTNVFYDLVRFFLPQLALVSMLSPFLFKIFHRIGWLHD
                     GYVRSFERP"
     gene            9782..11749
                     /gene="mrdA"
                     /locus_tag="PLT63_03710"
     CDS             9782..11749
                     /gene="mrdA"
                     /locus_tag="PLT63_03710"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOD97862.1"
                     /translation="MMGMSGRLSGHDASDYRLRFRRLFALVLIAFAVLIVRMAYLQVI
                     KGDEYKLRSESNSVRLRKLKPFRGLIMDSRRQVLVDNQPAFDLIYVPSRIMDISQITS
                     AVAGVYAKRGLTLSADLLALGKTKPFVPVRLERNINLEKIALVETHALDLPGVVVEVV
                     PVRKYLGGDIISHIIGYTGEISREELEEWESDEIGPGDIIGKAGLEKLLDPYLTGRPG
                     AEQVEVNVTGRVIKSLGSVVPVSGLNAVLTIDFDLQTVAANALEGRSGAAVVLDTRDG
                     SVLVLISSPSFDPNLFHGGISQRDWDKLARDPRHPMENRAVSGQYPPGSTYKIVTAAA
                     ALSEGLITPETRFYCNGSFSLGNRSYRCWQRHGHGWVNLHRALVESCDVYFYNLGKAL
                     GVDKLAEYARSFGFGVRTGIDLPREKPGLVPTREWKLKRFKEPWQPGENIPISIGQGF
                     DLVTPLQLAGAYAALANGGILWHPRLVKQIEEADGRIVKQFPPQQAGTVSLSAPHLEM
                     IRRGLWGVVNEPGGTGGALRRPEGDVAGKTGTAQVVGLPQDDRARRARILSGRFRDHA
                     LFACFAPHKQPEIAVTVIVENAGHGGSVAAPIARKIVDAYFQLKKERAAPKIAPAVPT
                     DSRYEGRKQPDAEQAEQLEAAREDEGGEEDE"
     gene            11787..12896
                     /gene="rodA"
                     /locus_tag="PLT63_03715"
     CDS             11787..12896
                     /gene="rodA"
                     /locus_tag="PLT63_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HOD97863.1"
                     /translation="MKFNKQWLLSFDWTLLVITLAVCTIGVLNIYSTGFSLTSAKLPL
                     YIKQIQWILIGLFFMAVIITLDYRLLSQYAYFLHGFAILLLLFVTFYGYATHGSQRWI
                     ALGGFTFQPSELVKLTLILSLARYFSVHGVNGTLYALKDLLVPLAIVLAPFILIVRQP
                     DLGTALMLLIIFLSMILFVGVRIKDLLVSGITGLLIIPLAWLFLKDYQKDRILTFFDP
                     ERDPLGAGYHIIQSMIAVGSGGLLGKGFLKGTQTQLKFLPEQQTDFVFSVFAEEWGFA
                     GGMVLVFLFLFLVLWGLKIARNSRDLPGTLIAYGITIMIFWGIFINIGMVLGILPVVG
                     VPLPFLSYGGSSVVVLMMSMGLLINISMRRFILLS"
     gene            13126..13554
                     /locus_tag="PLT63_03720"
     CDS             13126..13554
                     /locus_tag="PLT63_03720"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HOD97864.1"
                     /translation="MVDINLTLFIQMANFLVLIVILNYLLYKPILSILDKRKARLDES
                     EDEIKRLNLTVEQKAAEYEEKLRQAKQAALGKKSEILKEAADGAKAIIDEKRGKIPAM
                     LAEFQSRVSQEVEGARQVLKNQSQKISAEIAEKVLGRSLQ"
     gene            13551..14147
                     /locus_tag="PLT63_03725"
     CDS             13551..14147
                     /locus_tag="PLT63_03725"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HOD97865.1"
                     /translation="MRNDNRRQLIKSVLIAGSMVLVAGTVAYAAGGGGEEHHPIDWKD
                     FGYRMFNFIILAGFLWWLSAKKVKEFFAGRRNDIKESLNQAQAAKEEAERKYKEYVAK
                     LEKATDEIDGIAEMIKVQGMAEKEKMIEEAKKAAVKIEEDTKARMEQEFSTARNQLRA
                     EAAQLSIQLAEELLKRNITIQDHEKMVKEYMDKVVIKH"
     gene            14144..14689
                     /locus_tag="PLT63_03730"
     CDS             14144..14689
                     /locus_tag="PLT63_03730"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004402.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HOD97866.1"
                     /translation="MIGSTIAKRYARAFFEIALEEKQLEQYYKELSGFAAIISDNKDL
                     KEFLANPIFDQSEKKSVVESILQKTAVSGITANFLKLLTDKQRIVILSDIVECYRELM
                     DEALKTVRASVKTAFPLSSELVRKLQEGLENQTSKKVEMTVIEEPSLLGGIVVRVGDT
                     VYDNSIRTQLNNIRNLLGEEM"
     gene            14693..16204
                     /gene="atpA"
                     /locus_tag="PLT63_03735"
     CDS             14693..16204
                     /gene="atpA"
                     /locus_tag="PLT63_03735"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOD97867.1"
                     /translation="MDTIRAEEISQIISKQIKDYEKKLDISETGTVLSVGDGIARIYG
                     VQNAMAMELLEFPGGILGMVLNLERDNVGVAVLGEVTHIKEGDIVKRTGRIAQVPVGE
                     AVMGRVIDAVGAPIDGKGPIEATEFRRIEMVAPGVIQRQPVNQPMYTGLKAIDAMTPI
                     GRGQRELVIGDRQIGKTAICIDAIIRQKETGVKCIYVAIGQKKSTVAQVVENLRKYDA
                     MSYTCVVSACASDPATLQYIAAFAGCSIGEYFRDRGQDALIIYDDLSKQAVAYRQISL
                     LLRRPPGREAFPGDIFYNHSRLLERAARVSPDLGSGSLTALPIIETQAGDVSAYIPTN
                     VISITDGQVYLEPGLFFSGVRPAINVGLSVSRVGGAAQVKAMKQVAGTLKLDLAQYRE
                     LAAFASFGSDLDKATQAQLDRGVRLVELLKQPQFQPMSLGEEVAILFAGTKGFLDKYP
                     VDKIKDYENQLLSFVKSKYADILKEIDEKMIISPELEKKMKDALTEFDAVFAG"
     gene            16262..17137
                     /gene="atpG"
                     /locus_tag="PLT63_03740"
     CDS             16262..17137
                     /gene="atpG"
                     /locus_tag="PLT63_03740"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HOD97868.1"
                     /translation="MAALKDIKRQIAAVGKTKQITKAMNMVAASKFRTAQMRMENFRP
                     YAGKFIEVLNSLALRVDASSHPLLAVRPPKKLRVICMTSDRGLCGGFNTNLIKATERF
                     AKEKEAEGKEVSLVPVGRKGRDYFRKKAKIMNERVDVFKKFDMSLAVEISNDIIPSFV
                     KEEYDELYLIYNEYRNVSVQRPAVVRLFPLPSIGQEEATDPEKRIDYIYEPSDEVLLD
                     RILPMYVRVLIYRALLETYAGENGARMAAMDNATRNCEELIGSLSLKYNKARQAAITA
                     ELMDIVGGTEALAKG"
     gene            17168..18574
                     /gene="atpD"
                     /locus_tag="PLT63_03745"
     CDS             17168..18574
                     /gene="atpD"
                     /locus_tag="PLT63_03745"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOD97869.1"
                     /translation="MNIGKVVQVIGPVIDVEFEEGKLPAIMNSILITNPAINDQEDNL
                     IVEVAQHLGDNVVRCIAMDITDGLVRGMPAKDTGAPIMVPVGKECLGRILNVVGRPVD
                     GMGAIDAKNMMPIHREAPTFLEQNTSVHVLETGVKVIDLLVPFPRGGKMGMFGGAGVG
                     KTVVMMEMIHNIAMHHGGISVFAGVGERTREGNDLYREMLESGVIKQAALIYGQMTEP
                     PGARARVALTALAAAEYFRDVEGQDVLLFVDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATDLGELQERITSTTKGSITAVQCVYVPADDLTDPAPATTFAHLDGTVVLSRPIAE
                     LGIYPAVDPLDSTSRILDPNVVGEEHYSVARQVQVTLQKYKDLQDIIAILGMDELSEE
                     DKLTVSRARKIQRFLSQPFFVAAQFTGTEGKFVSVPDTVRAFKEILEGKHDDLPEQAF
                     YMVGGIEEVIEKAKRLSE"
     gene            18589..18996
                     /locus_tag="PLT63_03750"
     CDS             18589..18996
                     /locus_tag="PLT63_03750"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HOD97870.1"
                     /translation="MADELLLEIVTPEKMAFNGKVEEVTVPGSEGEFGVLRGHASLLS
                     SIQIGELSYTQNNKKVAYAVDTGYAEVLASKVTILVEGAERSDMIDRDQARKDKEEAE
                     AKLAKMAKEDADYERVKAAVTNAETRLKVAERG"
     gene            complement(19088..19570)
                     /gene="greA"
                     /locus_tag="PLT63_03755"
     CDS             complement(19088..19570)
                     /gene="greA"
                     /locus_tag="PLT63_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOD97871.1"
                     /translation="MKKAPITKVGFEKIKRELEHLKTVAVPENVRDIEAARAHGDISE
                     NAEYTAAKERQAFIHGKIQELENNLATSEIIDLKNLSTDRIVFGTSVIIEDVKKGKNI
                     TYQLVGPHESDIGRNKISVTSPLGKALVGKTIGDTARVNAPGGVREFEIVDIFIAHVD
                     "
     gene            19780..20772
                     /gene="moaA"
                     /locus_tag="PLT63_03760"
     CDS             19780..20772
                     /gene="moaA"
                     /locus_tag="PLT63_03760"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HOD97872.1"
                     /translation="MYDNYQRDINYLRVSITDRCNLRCRYCVPKEGLSLIGHDDILRY
                     EEILRIVRIAVGLGIAKVRVTGGEPLVRRGVITFMTALKVIPGIEDLSLTTNGILLER
                     YAADLRAAGVCRLNISLDSLNREKYYYITRGGSLANALRGIDAVFEQGFAPIKINVVA
                     MKGFNDDEILDFAHLTLSRPFQVRFIELMPVGHTGRDNLGKYLACEEIMGTIERNYPL
                     LPVNGARDRTDGPARIYRIDGGAGEIGFITAMSSHFCHQCNRLRLTAEGHLRSCLFSD
                     AEIDLKGPLRSGCGDDRIEALIKEAIANKPRKHEIRPEGNYIRECGKNMSAIGG"
     gene            20778..21545
                     /locus_tag="PLT63_03765"
     CDS             20778..21545
                     /locus_tag="PLT63_03765"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOD97873.1"
                     /translation="MIFTEEIARSYDNWRDTPPGRYIDSREKSLILDLTQPRGGERLL
                     DVGCGTGDHLWLFRRKGCDVTGLDASAPMLEQARRKLGAKADFYLGAAEDLPFSDNEF
                     DIVTLITALEFTADPEQALREAIRVCRGRVFIGVLNKFSCLGMARRISATFGPSLYNH
                     ARFFSIGALAAMIRRQLAGVRVRWGSVIFLPSGWYGFAAGLEEIFPVMNNPFGAFLGL
                     SFSVTFTQVTLQEPIRETLTIGAEGRRPVPGVVRVIK"
     gene            21542..22552
                     /gene="amrS"
                     /locus_tag="PLT63_03770"
     CDS             21542..22552
                     /gene="amrS"
                     /locus_tag="PLT63_03770"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOD97874.1"
                     /translation="MTREAMLFEPLADGQVRCLLCAHRCLIKEGRRGICGVRENCDGV
                     LYSLVYGAVIAENIDPIEKKPFFHLHPGSRSYSLATVGCNFRCRFCQNHEISQLPREA
                     GTVPGKNATPAEIVARAVDADARTIAYTYTEPTVFFEFAYDISRLARERGIKNVFVTN
                     GYLSEEALATISPYLDGANVDLKSFRDDFYRQQCGARLQPVLDTLVRMKTAGIWVEVT
                     TLLIPGLNDSEEEIDQIAAFLAGLGKETPWHISRFHPRYQMNDLPATPRERLQKAREI
                     GRAQGLKYVYSGNVPGDEGENTFCPGCGRLLIGRYGFCVESMMLRNAACMHCRTPLDG
                     VF"
     gene            complement(22549..22815)
                     /locus_tag="PLT63_03775"
     CDS             complement(22549..22815)
                     /locus_tag="PLT63_03775"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HOD97875.1"
                     /translation="MEKREEMEGGYWREVCRVGGLNIAHVIVGRLEADGIPARLRYEA
                     AGAIYAITIDGLGEVTIVVPEREWERAREILSRTYDEDELNWNG"
     gene            22943..26515
                     /gene="smc"
                     /locus_tag="PLT63_03780"
     CDS             22943..26515
                     /gene="smc"
                     /locus_tag="PLT63_03780"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HOD97876.1"
                     /translation="MKLKNLEIKGFKSFRDKVALDFAVGVSAVVGPNGCGKSNVVDAI
                     RWVIGEQRVKSLRGVRMEDVIFNGAQEAEPVGMAEVFMSLAAEGLAFPDPYHDCAEVV
                     ISRRLLRNGESEYAINGLPCRLLDVREFFMGAGVGTRTYSIVEQNSVASLVEAKPEER
                     RQFIEEAAGISKYKTRKEAAQRKLEATNQNILRLNDILREVKTQLNALSRQARRAEQY
                     RQLRNSLREDELAVARDAYGALHREQEERSRQLQNCRQREEELHTALMGCEASLAAAR
                     IEVLDREAERSRCQEHIYQLRTAVQVKEQGIEFTRGKIADLEARQQRLAEEIRQFQTR
                     HEELGRERERLAALQHILSTDMAEIERDVAERERRISTLKGDLAARQQRLEENKSRLI
                     DAAAEKSRLSNNCSHLLKGLEEITRKEERNAREIAEQRGQEERLREKVAGIETTLKDD
                     LHCLEELRRRREEGTVEWEDAVREMQETEERIDGLKEEFSSRTSRLASLKELHDGYVW
                     CNEATRSIMTAKAADSLKGVDRDQVIGLVVEQLRVPREYETAVEAVLGEKLQYVIVRS
                     QEAGVQAIDYLKEAAVGRTSFVPLAVRHSTPGVHAEHLRETERLLERIEVQDGFRGIA
                     EYLLGDVLLIPDLRNGLALWNRNGFRGTFVTPDGDTISPQGVLTGGRNGNGARSLLKN
                     KREIETLEEEIHGLSASLQTDTARRKELAARVVRSEEDLEEMTAAIHARELQWNSRQK
                     DLERFEAECKRLRQTRKVLEFNGESQAEERLLLDEKLQSGRAELENQRQREEAAREEA
                     AGLQEEVDQLRRDLETRAGELTEEKVRQASMAEQNKSVVKQWERLQAEVADLKETMRK
                     GGDDSLSWTRQLEELRKAAVRDKAELMELYAALAELEKELADKGNLHALAETSLRDWE
                     GKRQETKERYERIGREGRKLESEVRELDYQLDGLIRMCAEKHQIDLTESPLTEPPLSE
                     EERRLREDRIEKNRRSAAAFGEVNLLAINEYEQLQERFAFLNSQSRDLNASLNTLQQT
                     IAKINRVSRQRFAETFEAVRGSFRDVFSRIFPGGKGELYLTDESDLLETGVDIDIRIP
                     GKRSQNISLLSGGEKSLAAIALILAIILYRPTPFLVLDEVDAALDDANISLFAKIIRE
                     IAVNSQVILITHNKKTMEVAENLFGVTMQKQGISTVVSVSLNRS"
BASE COUNT         5729 a         7364 c         7990 g         5494 t
ORIGIN      
        1 gggagttgaa gcaggtcgtc gccgagcttg cccaggcgca gaagcgcacg gaagaacggg
       61 tggaggagct tgcccaggcg cagaagcgca cggaagaacg ggtggaggaa cttgcccagg
      121 cccagaagcg cacggaagaa cgggtggagg agcttgccca ggcgcagaaa cgcaccgaag
      181 aggcggtata tcatttatcc cgccgcctcg atgacacgaa ccgccagctt ggcggtctgg
      241 ccatgaccgt gggttatacc ctggaaaata gcgcttatgt ttatctcccc gccctcctga
      301 aggccgacta cggagtatcc tgcgaagagc ctctgtaccg ggactatctg accgataata
      361 aaggtgcgga tgtcgaggta aatatcttgg gccgggcaaa gaaggatgca caggatctgc
      421 tcatcgtcgg cgcaagcaag gcgcagctca gcaaaaaaga cgtccaggca tttatcaagc
      481 gcattacccg catcgatacc gggggcctta gaccgtttcc ggttctcgtt acccacatga
      541 ttagccagcg cgatgtgaag aactatgcgc aaagccaggg cgtggcgatt tatctttcct
      601 atcagtttca aaatcctgtc cattgagcga agatttagga tcctcgttga caatcttccg
      661 cgccggattc catgagaatt taggccagca gccggctgtt gtcatccggg ggttcattcc
      721 cagttaaagg gatcatcgcg gtcgaaatcg gggctcaggt ggcggtcgtc cacctcctgg
      781 acgaagagct gggagaagcc gagcgccagg gcgtagtcca cgacccgccg gtattcgtcc
      841 cggcggatcc gccggcccag gaggggatgg cggcaggcct gggccgccgg cgtatattgg
      901 gacatgatgc tcagcgacac gccggtggag acggaggcgc ggatgtcgtc caggacggtg
      961 aaactgtttt ccaggcatcc ggggaggacg agatggcgga tgaggagccc ccggacggct
     1021 accccctccc gcagctcgag accgtcgccg acctgccggg ccatctcggt cagggccgcc
     1081 cgggcatggc ggcggtattc ctttaccccg gaaaactcct gggcgagatc gtcgtcgcca
     1141 tacttcatgt ccggcatgta gatctccacc cgtccggcga gaaggcggat tacctccaga
     1201 gattcatacc cgccgcagtt atggatgtag gggatcgtca atccccggcg gcgggcgatg
     1261 tccagggcaa ccatgatcgg gggcgtctgg tgggtcggcg tcacggcgtc gatattgtga
     1321 cagccccgct cctgaaggcg aagcatgacc tccgccagct cctccggggt caggatgctg
     1381 ccggtggtct tcctgctgat ctggtgattc tgacagaaga tgcaccggag attgcaggag
     1441 gagaagaaga tcgtcccggc gccgtggcgt cccgacaggg gcggttcctc gccgtagtga
     1501 ggcatggcgt cactgacgac gatttccgtc cccagtccgc aaaatccctt tgctccgccc
     1561 cggcgctccg cccggcactc ccggggacag aggacacagg gcgacatgag tcgttccagt
     1621 tgttcaagat cctgatttgc atcggtattt tccatttgcc gatccttctt gccgcccctc
     1681 ttttcggaac cgcgggtcaa gagcatcccc gccccgggct gcccccggaa atcgccccca
     1741 tgtgtttgca gtgatgatcg tttccgctcc gatgcggtgc gcctcgatcc caaaattctg
     1801 attatctaca cgaagcttcc cgttgtcaag gcgtcaaggc ttcggtcgtc cgtctcccgt
     1861 gccgttatcg gtaagcggcc ggacgagctc ccggattctc ccgccggcga caagctccag
     1921 aaagatctcg cccagcggtt ccgcggcggc aaccgcgcgg cgccagttgg caatccgggt
     1981 ttgcggatcg tcgaggaagg tgacgaaatc gtcccggtcg gggacttttc ctcccggcag
     2041 ggaggcgaca aattcctccg ccggatggac caggatcaga ttgtgaaggt ccgccgccgc
     2101 gggcttcctt ttgacgagcc ttttgtcgag ccagccggga atgaggcgat cctggtggag
     2161 gaacaggagg gtcagatcgt tttcgcccgc cccgaaattc tgggccaggt ggtagtccac
     2221 gagccccccg tcccgataga cgccccgggg cgccccgtaa atatcccgga tcccggcgac
     2281 gaggagcggg atcgcccccg aggccaccag ggccggtttg aaattggtgg tgttgagagg
     2341 tacataaaca ccccggaaat ccggccgcaa ggtgaaggcc gggggtttgg ggccgctgaa
     2401 aaagaccacc cgttcctgaa agttatagag ccaggaccgc ttcaccgtat tggcgagaaa
     2461 ggcgccggcg aatcccagtc gctgtagcag gggatttttg gaagcggcga ggcgcctgga
     2521 acgggccgtg atgacggcca ggcggtagtt gcgggagttc agggcgaagg gcaaggcgtc
     2581 gttttcgatg gcccggtcga tgatggagat gagggattcc cggatggtgg ttgggcggtc
     2641 cgtccgggta tatatcgttt cgatgtaggc cttccgaagt gcgcgatagc tcttttccgc
     2701 ctccggctga atccaggcgg cgaagcgcca ggcccccgcc gaggccccga tgagggtcag
     2761 gggtcgcgtc cggcccagga cctgctcctt gatgagggtc aggtcgaagc cgccggcgat
     2821 taaccagcgc ggacccaccg ccggaccggc gtaacaggtg acggcatcca gggtgaagcc
     2881 tccatcgcga atccgccgga aagcccccgg tccggccttg acgcagagcc tgttcatagc
     2941 cgcccctttc ctcaaatcac cttgacacaa gcggtgtggt tttgtagagt gatccagttt
     3001 tcctcccatc ctccgtcggg aggtgggtat tgatgcaaaa cgagggcgcg ttaataggca
     3061 gaatttgttg actttgtcaa tgggagagcc ataaaaaaat gtctgttcga acccaagtgc
     3121 tcacgatggc cagggaggca aaaatggccg ccgccgtctt gagcgttcta tccgcggcgg
     3181 ataagaaccg gatcctccgg gcggtaggag cggccctacg ggaaaatgcc tcgtatctgc
     3241 aggaggaaaa tgcccgggat gtagccgcgg cggcggaaaa gggcctgccg gcggcgatga
     3301 tcgatcgcct cactcttaag gaagcgacca ttgcggcgat ggccgcggga tgcgacgagg
     3361 tggcggccct cgacgatccc gtgggcaaga tcacctccat gtggcgccgt cccaacggcc
     3421 tgctggtagg tcggatgcgc atccccctgg gggtgatcgg catcatctat gaatcccggc
     3481 ccaacgtcac ggtggatgcc gccgccctct gcctcaagtc ggggaacgcc gtgattctcc
     3541 ggggcggctc cgaggccatc aattccaatc gggccgttgc cggcatcatc acggacgtgc
     3601 tgaagagagg gcctctacct ccggatgcga ttcaactcgt tcccatgacg gaccgggagg
     3661 cggtttacga gctgctccag ctcgaggaat ggatcgatct catcatcccc cgggggggag
     3721 aggatctgat ccgcaccgtg gtgagccact cgaagatccc cgtcatcaag cattacaaag
     3781 gtgtttgcca cgtcttcgtg gatgagaccg cggagttgga gatggcggtg cggatctgca
     3841 tgaacgcgaa ggtccagcgc cccggggtct gcaacgccct ggagacgctc ctggtgcatc
     3901 gggaaattgc cccccggttc cttccccttt tggcggaaca atatcgacag gcgggcgtca
     3961 cgctccgcgg ctgccccgtg acgcaggaaa tacttcccgg agttgccacc gcaacggagg
     4021 cggactggca cgcggaatat ctcgacctga tcctggccgt aaaagtggtg gaagatctgg
     4081 acgccgccat ggaccacatc cggcgctacg gttccctcca taccgaggcg atcgtcacga
     4141 gcgattacgg ccgggcccag cggtttcttc aggaagttca gtcctcgacg gtgctcgtca
     4201 atgcctcgac gcggttcagc gacggattcg agctggggct cggggcggaa atcggcatca
     4261 gcaccaccaa actccatgct tacgggccca tgggactgga agagttgacg acctccaaat
     4321 tcatcattta cggaaacggg caggtgagaa catgaaatgg ggactgctgg gcgggacctt
     4381 cgatcccatc catatcggcc atctgcgttg cgccgaggaa atcctggaga tattcgatct
     4441 gaaccggatc atgtttatcc ccgcttcccg gccgccgcac aaaatcgacg gcgaaataac
     4501 ctccttctac caccgggagc agatggtccg gttggccatc gaaaacaatc cttccttctc
     4561 cttttccgat gcggaaaaca ggaaggaagg caaatcctat tctgtggaga ccgtggagtt
     4621 catcctcaag aagtacctgc agaacctgga actgtacttc atcgtcggcc aggatgcctt
     4681 tcacgccatc accacctgga aagactggga aaagctgttg cagctatgta atttcgtcgt
     4741 gatgaccagg ccgggatacg agaacaaggg attggccgga atcctgccgc cggagttttc
     4801 ctcccgcttt cactacgaag cccgggaaaa cggttttcgg ggacccacgg gctacatgat
     4861 ctatttccgg gaggtcactt tcctggacat ctcctcgagc cgcatccggc agcgggtccg
     4921 ggacggcaaa tccgtcacct acctgaccac ggacccggtg cgccactaca tcatcaataa
     4981 aggtctctat cgcagcctat aaaacgactt cggaaacgga aggaggttaa gcggcatgca
     5041 agtccttgtt tacggagctt tatccgcggt tcttaccctt gtgtttctgc tgtcttcccc
     5101 cccgcccggc catgccttcc gttgtggcag cggcatggtg agcgtgggcg attccaaggg
     5161 gaaggttcta atcgagtgcg gcaagcccac cttcaaagag aaggtggcgg accgggagac
     5221 ccgctatgcg tcaacgggaa aaagcacgaa agggaaaagc ggcaagacca ccaaaaccgt
     5281 tgagcagtgg acatacaact gcggcaagga cgatttcatc tatatcctga ccttcgaggg
     5341 aggcaaactc atccgggagg atacggacgg ccggggccgc ggcgaatcgg aatgcaaggg
     5401 ccgttgatga cgaaaccggt aaagccgggg atttcaacag gaaccggaat cagagctctt
     5461 ttgcctccct gttgatcttc agttttccct ctttgatcat ggcgctttta ctcgcctcga
     5521 tccaggtctg aatcgcttcg ttccttttca ggtgcaggca ggccctcctt atctgctctt
     5581 tctggtccgc cgggagttcc tgttccgact tttcccgccc ccggagctcg acgaggagat
     5641 gatcgccatc gatcgtaaat atctcgtccc cgtaaggttt tttgggggag agtaggaaga
     5701 gtgcccttgt caattggccg gacgatccaa tccgggggac cgggccggtg ggaaggaaga
     5761 agcccgtctc ctcgattttg agcttcatgt cccggccgat atccgccagt ttttcacctt
     5821 tccgcaggcg ggctagggcg gcatccgcct cctgcccggc ccggcgccgc ccctccgcaa
     5881 ctatatagcg gctccggatc tctttttcga tctccttgag ctctggggta taagcgggtt
     5941 tccttgcccc ggccttgatc aggtaatagc ctcgttccga ggagatgacc ttgcttagtt
     6001 catccttctg gagggaaaag acggttgggg cgaaatcggg gagccgccgg aaaatctctg
     6061 ggggttcctt ttcggaaaag aatcccgttt ctgtcacctt caggccctgg gcggcagcgt
     6121 aggcttcaaa gttttcctgt tgatatatcg tttcccgggc ctggcgggag gcctcgaaag
     6181 ccagcttcat cgcctgggcg cgcttgatct cggcgatgat gcgatcccgg acaccggcga
     6241 gcggcacggt cttgccgtcc tttgtccatt gttgcttgcg gttttcgtag aaatcggcaa
     6301 tctcgttgtc cgtaaccttc accttgcccg tgaagtcctc cggggcaaac aacaggtact
     6361 tgatccggat ctgctcgggg gtgcgaaaat cgccctggtg ctccttccgg tacttttcca
     6421 attccgtggt tccggcttgg accctgccgg caaaatcgcc gccccggaac cggatgaagg
     6481 cgaggttgat cttttcgctc tgggcctggt aaaagttccg cacctcctga tcggaaacat
     6541 ggatgccgtc ctgaatcagg tcctcgacct tcatgatcag cagcgatttc cgctggtcgt
     6601 tttcaaagtc ttccggggtg attttattgt aacgcaacat ctgctgataa aggcgggaat
     6661 cgaaggcacc gtcccgctga aaggcgggga ttagggagat gtaggtgcgg atttcgtcgt
     6721 ccgtgaccat gatcttgaga tcctgagcct tcttcatcaa gacggcctgg taaatgagct
     6781 tgttgaaggc ctgctccttg agattcaaac ccttcatcat ctcttccgtc attctacccc
     6841 cgaaacgacg gttgtacata tcgacaaggc ctcgatactc cttttcgtat tccatatagg
     6901 tgatgattct gccgtcaacc gtggcgatgg cctccgcctt ttgccgcccg gtcatggatc
     6961 cgaagtagaa gataaagacg atgatgatga tggcgaggag gagcttcatg agccagttgc
     7021 gggcatgttt tctcatcagt tcaagcatgg gtttcctctt ggcaagtttt ttaattcggt
     7081 atggttatta tagtcccaat caaaatgcaa tcaccttttt aaaaaggcat tgcttaggtg
     7141 ggggaaatag gctataggat tacgccagta cgctttttac aaagcgtaaa cataataatt
     7201 tattgagaaa acacaggagg ccgcaagcgt gctttttgat tttattctgg gaaaattctc
     7261 caacgacctg gccatcgatc tggggacagc caataccctg gtgtacgtga aggggaaagg
     7321 tatcgtcctg agcgagccgt cggtggtggc ggtgcatatg gattccaaag gcgtcaagaa
     7381 ggtgctcgcc gtgggagcgg aggccaaaaa gatggtgggg cggaccccgg gtaacatccg
     7441 ggcgatcaga cccatgcggg acggcgtcat cgccgatttc gacattaccg aggaaatgct
     7501 ccggcatttt atcctgagcg ttcataaccg ccgcaccctg gtgcggcccc ggatcattgt
     7561 ctccgtgcca tcgggcatca cccaggtgga gaagcgggcg gttcgggaaa ccgtggagtc
     7621 cgccggcgcc cgggaaatat atcttatcga ggagccgatg gcggcggctc tcggggcggg
     7681 cctgccgatc accgaaccga cgagttccat ggtcgtagat atcggcggcg gcacgaccga
     7741 agtggcggtc atctctcttt cgggcatcgt gtatgcgaaa tcgctccgcg ttgccggtga
     7801 caagatcgac gaggagatcg tccagtacat gaagagaaag tacagcctcc tggtaggtga
     7861 acggacggga gaaatcatca agacgaccat cggttgcgcc tatccggaca aagaaatccg
     7921 gaccgtcgaa gtgaaggggc gggatctgat ctccgggatt ccgaagatca tcgagataaa
     7981 ttcggaagag gtgcgcgagg ccattgcgga accgatcagc ctcattgtgg acaccgtcaa
     8041 ggatgccctg gaaaatgccc cgccggagct ggccggcgac atcgtggacc ggggtatcat
     8101 gctcaccggt ggcggcgccc tcctgagaaa tctcgatgtc ctgatccggg aggaaacggg
     8161 actgcccgtg accatcgccg atgatcccct ctctaccgtg gcccgtggcg ccggaatggc
     8221 cctcgaccag ttggacgtgt tgaaggaggt caccctgcag atataatgtc cgggcaggcc
     8281 gtcaggttgc cgctggcgga tcgctgccga ccccggaggt ggcgtcatcc gccgatccgc
     8341 ctcatcaata atatgtgtgc attgttcatg cagttgtttg cgggtggtga cgggaaccgt
     8401 gttcccccga ccatcacctt gacgaatggc cgccccggcc ggccgccccg gtccgataaa
     8461 tgagccgcga gttttcagaa agatgccgat tccgaaaaaa taccggttca tgaccgtaat
     8521 catcgccgtc gtcgccattt ctgtagttat tctcctgatc agcgcgaaag gctcatatga
     8581 taccggactt ccgaggaaga tcatcctaga tgtggccgcg ccgatggaac gcatcctccg
     8641 ggcgccgctg gatggaattg ccgagatctg gtcccgatac ctgtttttgg tgggcacggg
     8701 cgaggaaaac cggcggctga agaatgaaaa cgaccggttg aagaatcagt tgctccaata
     8761 tgaggaaggt tatctggaag ggaagcggct gcaaaaactc ctggccctga aggaacggag
     8821 cgaatatgaa gccctgccag cccggattat cggtatcgac cagaaatctc tgctcaagac
     8881 gatcctcatc aacaaaggtt ccgcgcacgg tatccggaac ggcctccccg taatcaacga
     8941 ccagggggtc ctgggaagga tcatcgagac ctcctggcac gtctcgcggg ttctcctgat
     9001 cagtgacggt aacagcaata tcgacgccat gatccagggc agccgggtgc agggtatctt
     9061 acagggaacg ggggcaatgc gctgcacgtt gaagtacatc gccaaaacag aagaggttaa
     9121 agaaggggat ttggtggtct cctcgggaat gacggagatc tttcccaagg gattgctgtt
     9181 gggaaccgtc atccgggcgg gcaagaaggc acccgggctg tttcagacca tcgaggtggc
     9241 cccggcggtt gacttcacca ggatggaaga ggcacttgtg cttgtagttc ccgaggtcat
     9301 gaaaaaatga tcttttacct cgtcgtgccc tggtttttga tgattgtggt agtgttccag
     9361 cacgccgttt tgggagcggt gtttttccat tccatcgccg tcgaaatatc cctggtcctg
     9421 gtcatctacg cgggtctgcg gatgaaagtc cttcggggcg ccatcctggc tttgatcctc
     9481 ggtttgatga tggattgcat tacgggtgcc gggtccgggt cttacgcctt tgtctatacc
     9541 atggtgtatt ttatggttta cctactgtca gtccatgtct atgtggaaaa cgcccctttt
     9601 ttgatcttcc ttaccctggt ttgcggtctt gtggaagggt tgctgctcct ggtcctgaat
     9661 cggatgctct acggcacgaa tgtcttttac gacctggtcc ggttcttcct cccccaattg
     9721 gcgctggtaa gtatgctcag cccgtttctg tttaaaatat tccaccgtat aggttggctt
     9781 catgatgggt atgtccggtc gtttgagcgg ccatgatgca tccgactacc ggttgcgctt
     9841 ccggcggctc ttcgccctgg tcctgatcgc ctttgccgtc ctgatcgtca gaatggccta
     9901 tctgcaggtg atcaagggcg acgaatacaa actacgttcc gaaagcaaca gcgtccgcct
     9961 gcgcaagctc aagcccttcc gaggattgat catggattcc cggcgccagg tgctggtgga
    10021 taatcagccc gctttcgacc tgatttatgt accgagccgg atcatggaca tcagtcagat
    10081 tacctccgcc gtggccggcg tctatgccaa gcgggggctg accctgtcgg cggatttgct
    10141 agccctgggg aaaaccaaac cttttgtccc ggtccgactg gaaagaaaca ttaacctgga
    10201 aaagatcgcc ctcgtggaaa cccacgccct tgatctgccc ggagtggtgg tggaagtggt
    10261 accggtgaga aaatatctcg gtggtgatat catctcgcac atcatcggtt ataccggcga
    10321 gatcagcaga gaggagttgg aagaatggga aagtgacgag atcggaccag gagacattat
    10381 cggcaaggcc gggttggaga agctcctgga tccctatctg accgggaggc ccggggccga
    10441 gcaggtggag gtgaatgtca ccggccgggt gatcaaatcc ctggggagtg tggtgccggt
    10501 atccggattg aatgcggtat tgaccataga tttcgacctc cagaccgtgg cggcgaatgc
    10561 cctggaggga agaagcgggg cggcggtggt tctggacacc cgggacggtt ccgtcctggt
    10621 cctgatcagt tccccgtctt tcgaccccaa cctgttccac ggcggaattt cgcagcggga
    10681 ctgggacaaa ctggcccggg atccccgaca tcccatggaa aaccgggccg tctccggaca
    10741 gtatcccccc ggatcgacat acaagatagt gacggcggcg gcggccctct cggaaggttt
    10801 gatcacgccg gagacgcgct tttactgcaa cggttctttt tccctcggga atcgatcgta
    10861 ccgctgctgg cagcgtcatg gccacggctg ggtgaacctg catcgggccc tcgtggaatc
    10921 ctgcgacgtc tatttctaca acctcggcaa ggccctcggc gtggataagc tggccgaata
    10981 cgcccgttct ttcggcttcg gggtccgtac cggcatcgat ttgccccggg aaaagccggg
    11041 actggtgccc accagagaat ggaaattgaa aagattcaaa gagccctggc aacccgggga
    11101 gaacattccc atatccatcg gccagggctt cgatctggta acgccgctcc agcttgccgg
    11161 cgcctatgcc gccctggcca acggcggcat cctctggcat ccccgtctcg tgaaacagat
    11221 cgaggaggcg gacggaagga tcgtcaagca attcccccca cagcaggccg gcaccgtgtc
    11281 actgtcggcg ccgcatctgg agatgatcag gaggggactc tggggggtgg tcaacgaacc
    11341 ggggggcacc ggcggggcgc tgcgccgtcc ggagggggac gtggccggca aaaccggcac
    11401 cgcccaggtc gtgggattgc cccaggatga tcgggcgcgg cgggcgagga tactttccgg
    11461 ccgcttccgg gatcacgccc tatttgcctg tttcgcccct cacaaacaac cggagatcgc
    11521 cgtgacggtc atcgtggaga atgccggtca cggcggctcg gtggctgctc ccattgctcg
    11581 gaagatagtc gatgcctatt tccaactcaa aaaggaacgg gcggcgccaa aaattgcccc
    11641 ggcggttccc accgattcgc ggtatgaagg caggaagcag cccgatgcgg aacaagcaga
    11701 acaactggag gcggctcggg aagacgaggg cggggaagag gatgagtgag tatgcggccg
    11761 cagatttatt agtgagataa aacgagatga aattcaataa acagtggtta ttgagctttg
    11821 actggacact actcgtcatc acccttgccg tttgtaccat cggcgtcctg aacatttaca
    11881 gcaccggttt cagcctgacc tcagcgaagc tgccccttta tatcaagcag atccagtgga
    11941 tcctgatcgg tctttttttc atggccgtca tcatcaccct cgattatcgg ctgttgagtc
    12001 aatatgcata tttcctccac ggttttgcca tcctgctttt gcttttcgta acattctacg
    12061 gatatgcgac ccatgggtcc cagcgctgga ttgccttggg gggctttacc tttcagccct
    12121 cggaactggt caagcttacc ttgattctct ctctggcccg gtatttcagc gtccatggtg
    12181 tcaatggaac tctttatgcc ctcaaggatt tgctggtgcc gctggcgatc gtcctggcgc
    12241 ctttcatcct catcgtccgc cagcctgatc tgggcacggc cttgatgttg ttgattattt
    12301 ttttgtccat gatccttttt gtgggcgtcc ggatcaagga tctgcttgtc agcggaatca
    12361 ccggcctgct cattatcccc ctggcctggc tttttctcaa ggattatcaa aaagatcgga
    12421 ttttgacctt tttcgatccg gagcgcgatc ccctcggcgc gggctatcac attatccagt
    12481 ccatgattgc cgtcggttcg ggaggcctgc tcggcaaggg ttttctgaag gggacccaga
    12541 cccagttgaa attcctcccg gaacagcaga cggattttgt cttttctgtc tttgccgaag
    12601 aatggggttt tgccgggggc atggttctgg tgtttctttt cctcttcctt gttctctggg
    12661 gtttgaagat tgcccggaac tcccgagacc tgccggggac cctcatcgct tacgggatta
    12721 cgatcatgat tttctgggga atttttatca atatcgggat ggttttgggc attctgcccg
    12781 tagtgggggt tcccctgcca tttttgagtt atgggggatc ttccgtcgtc gtcctcatga
    12841 tgtccatggg attgctgatc aatatcagca tgcgaagatt tatcctgcta tcgtgaccct
    12901 ggacggtctt tggccaaaaa aagcttgaca agcaacacga cttatgatta gtaagccgcg
    12961 cttttggcaa gggaggttcg ggttttgtat ttattcttac atgaccgcgg ttttggtgtt
    13021 atcgggctta ttgtagaaac tgaatattga cgaagatacc gggtggaagg gcatccctct
    13081 gcatccggtt tttttttgaa acagccataa tcgagaggta aaaaagtggt cgatataaac
    13141 cttaccctat ttatccagat ggccaacttt ctggtcctga tcgtgatcct gaactatctg
    13201 ctttataaac ccattctctc cattctcgac aagaggaagg cgcgcctcga tgaatcggag
    13261 gatgaaatca agcgcctcaa tctgaccgtc gaacagaagg cggcggagta tgaagagaaa
    13321 ctccgtcagg ccaaacaggc ggctttgggt aaaaaaagtg aaatccttaa ggaagccgcg
    13381 gacggcgcta aggccatcat tgacgagaag cgtggcaaga ttccggcgat gctggcggaa
    13441 tttcagagcc gggtgagcca ggaagtggag ggcgccaggc aggtcctgaa aaatcaatcc
    13501 cagaagatat cggcggagat cgctgaaaag gtattgggaa ggagtctcca atgagaaacg
    13561 acaatcgtcg gcaactgatc aaatccgtcc tgattgccgg gtcgatggtt ttagtggcgg
    13621 ggacggtcgc ctatgccgcc ggcggcggcg gggaggaaca tcatcccatc gactggaagg
    13681 acttcggcta ccggatgttt aatttcatca tcctcgccgg tttcctgtgg tggctttcgg
    13741 ccaagaaggt taaggagttt tttgccggcc gacgcaacga catcaaggaa tcgctcaatc
    13801 aggcccaggc cgccaaggaa gaggcggaaa gaaaatacaa ggagtatgtc gccaagttgg
    13861 agaaggccac cgatgagatc gatggcatcg cggagatgat caaggtccag ggcatggcgg
    13921 aaaaagagaa gatgatcgag gaggccaaaa aagcggccgt caaaatcgag gaagatacca
    13981 aggcccggat ggaacaggaa ttcagcacgg cccggaatca gttgcgggcc gaagcggcac
    14041 agctctccat tcagcttgct gaagaactcc tcaaacggaa tatcacgatt caggatcacg
    14101 aaaagatggt taaagaatac atggataagg tggtgataaa acattgatcg gaagcacgat
    14161 tgccaaacgg tatgcccggg cgttttttga aattgccctg gaggagaagc agcttgaaca
    14221 gtactataaa gaattgagcg gctttgcggc gattattagt gataataagg atcttaagga
    14281 gtttctggct aatccgatct tcgatcagtc cgaaaagaag tccgtggtcg agagcattct
    14341 gcaaaagacc gctgtatcgg gcataacggc caattttctc aaattgctta cggacaagca
    14401 acggatcgtc attctttccg acattgtgga atgctaccgg gagttgatgg acgaagccct
    14461 gaaaacagta cgggccagcg tcaagaccgc gtttccactg tcttcggaac tggtgcgcaa
    14521 gctacaggaa ggactcgaaa atcagaccag caagaaagta gaaatgaccg ttatcgaaga
    14581 gccctccctc ctcggcggga tcgtggtgag ggtaggggat accgtctatg ataacagtat
    14641 caggacccaa ctgaacaaca taaggaatct cttaggggag gagatgtagc gcatggatac
    14701 aatcagggca gaagaaataa gtcaaatcat atccaaacag atcaaggact atgagaagaa
    14761 gctggacatc agcgaaaccg gaacggtcct ttccgtcggt gacggcatcg cgaggatcta
    14821 cggcgtccag aacgccatgg ccatggaact cctggagttc ccaggcggca tcctcggcat
    14881 ggttctcaac ctggaaaggg acaatgtcgg cgtcgccgtt ctcggtgagg tcacccacat
    14941 taaagaaggc gacatcgtca agagaaccgg tcggatcgcc caggtgcccg tcggcgaagc
    15001 ggtcatgggt cgggtcattg acgccgtcgg cgcgcctatc gacggcaagg gtcccatcga
    15061 agccacggaa ttccgccgca tcgaaatggt ggcccccggc gtcattcagc gccagcccgt
    15121 caatcagccc atgtacaccg gcttgaaggc catagacgcc atgaccccca tcggcagggg
    15181 gcaacgagaa ctggtcatcg gcgaccgcca gatcggcaag accgccatct gtatcgacgc
    15241 catcatccgc cagaaggaaa cgggcgttaa atgtatctat gtcgccatcg gccagaagaa
    15301 atccaccgtg gcccaggtgg tggagaatct ccgcaaatac gatgccatgt cttatacctg
    15361 cgtcgtttcg gcctgcgcca gcgaccctgc caccctgcag tacatcgccg cctttgccgg
    15421 ttgcagtatc ggcgaatatt tccgggatcg gggccaggac gccctgatca tctatgacga
    15481 tctctccaaa caggccgtgg cctaccggca gatctccctc ctgctccgcc gtccccccgg
    15541 acgcgaggcc ttccccggcg acatcttcta caaccactcc cggctcctcg aaagggcggc
    15601 ccgggtcagc ccggatctcg gcagcggttc ccttacggcc ctgcccatca tcgagactca
    15661 ggccggcgac gtttcggcgt atattccaac gaacgtcatt tccatcactg acggccaggt
    15721 ctatctcgag ccgggcctct ttttctccgg cgtccgtccg gcgatcaacg tcggtctctc
    15781 cgtctcccgc gtcggcggtg ccgcccaggt caaggccatg aagcaggtgg ccggcaccct
    15841 gaaactcgac ctggcccagt accgcgaact ggcggccttt gcctccttcg gcagcgacct
    15901 tgacaaggcg acccaggccc agctcgatcg gggcgtccgg cttgtggaac tcctcaaaca
    15961 gccccagttc cagcccatgt ccctgggcga ggaagtagcg atcctctttg cgggcacgaa
    16021 ggggtttttg gacaagtatc ccgtggataa gatcaaggac tacgaaaacc agctcctctc
    16081 gtttgtcaag agcaagtacg cggatatcct caaggagatc gatgagaaga tgatcatcag
    16141 cccggagctg gaaaagaaaa tgaaagacgc cctcacggag ttcgatgccg tctttgccgg
    16201 ctgatggagt tcgtgaaacc gcggataacc tcaagttgat ttgaaaaggg agtaaaagtt
    16261 aatggccgca ctcaaggaca ttaaaagaca gatagccgca gttgggaaga cgaagcagat
    16321 taccaaggcc atgaacatgg tggcggcctc caagttccgg acggcccaga tgcggatgga
    16381 aaacttccgc ccctacgccg gtaaattcat agaggtattg aacagcttgg cgctgcgagt
    16441 cgatgccagc tcccatcccc ttttggcggt gcgtccgccc aagaagctcc gggtgatctg
    16501 tatgacctcg gatcggggat tgtgcggcgg tttcaatacc aacctgatca aggccacgga
    16561 aagatttgcc aaggaaaagg aggccgaagg gaaggaagtg tccctggtgc cggtaggcag
    16621 aaaaggtcgt gattacttcc ggaagaaggc caagatcatg aatgagcggg tagatgtctt
    16681 caagaaattc gacatgagcc tagccgtgga gatctccaac gacatcatcc cttccttcgt
    16741 gaaagaggaa tatgacgagc tgtatttgat ttacaacgaa tatcggaacg tttccgtcca
    16801 gcgtcctgcc gtggtgagac tcttccccct gccgtccatc ggccaggaag aggcgacgga
    16861 tccggagaag cgtatagatt acatctatga accgtcggac gaggtgcttc tggacaggat
    16921 actccccatg tatgtgcgag tgctcatcta tcgtgccctt ctggagacct acgccgggga
    16981 aaacggggcg cggatggcgg ccatggataa cgccacccgt aactgtgaag agttgatcgg
    17041 ttcattgtcg ctaaagtaca acaaggcccg gcaggcggcg attaccgcgg agttgatgga
    17101 tatcgtcggc ggtaccgagg ccttggcaaa aggatagtaa cactaaccac tttttaagga
    17161 gatgggtatg aatataggaa aggttgtgca ggtaatcggg ccggtcatcg acgttgagtt
    17221 tgaggaaggc aagcttcccg cgatcatgaa ctcaatcctc ataaccaacc ccgctattaa
    17281 tgaccaggaa gacaacttga tcgtcgaggt cgcccagcat ctgggcgaca acgtcgtgcg
    17341 ctgtattgct atggatatta ccgatggtct ggtccggggc atgcccgcga aggataccgg
    17401 ggccccaatc atggtgcccg tcggcaagga atgcctgggc cggattctga acgtcgtggg
    17461 ccgtcccgtg gatggtatgg gggcaattga tgccaaaaac atgatgccca ttcaccgtga
    17521 ggcccctacg ttcctcgaac agaatacctc ggtgcacgtc ctcgagacgg gcgtcaaggt
    17581 tatcgatctc ctcgtaccct tcccccgggg cggcaagatg ggtatgttcg gcggcgccgg
    17641 tgtgggcaag accgtcgtca tgatggagat gatccataac atcgccatgc accacggcgg
    17701 catctccgtg tttgccggcg tgggcgagcg gacccgcgaa ggaaacgacc tttatcgtga
    17761 aatgctggaa tcgggcgtca tcaagcaggc ggccctgatc tacggccaga tgaccgaacc
    17821 ccccggagcg cgggcgagag tggccctcac ggccctggcg gcggcggaat acttccggga
    17881 tgtggaaggt caggacgtgc tcctcttcgt ggacaacatc ttccgtttca cccaggccgg
    17941 ttccgaggtt tcggccctcc tgggcagaat gccctccgcc gtcggttacc agcctaccct
    18001 ggccacggac ctcggtgaac ttcaggagcg catcacctcc accaccaagg gctccatcac
    18061 cgccgtccag tgcgtgtacg tccctgcgga cgaccttacc gacccggccc cagccacgac
    18121 cttcgcccat ctcgacggca ccgtcgttct ctcccggccc atcgccgagc tcggcatcta
    18181 tcccgccgtg gaccccctgg actccacctc ccgcatcctc gatccgaacg tcgtcggaga
    18241 ggagcattac agcgttgccc gccaggtgca agtaaccctg cagaagtaca aggacctcca
    18301 ggatatcatc gccattctcg gtatggacga actctccgaa gaggacaaac tcaccgtcag
    18361 ccgggcccgg aagattcagc gcttcctctc ccagcccttc ttcgtcgccg cccagttcac
    18421 cggcacggaa gggaagttcg tttccgttcc cgatacggtt cgggccttca aggaaatcct
    18481 cgaaggcaag catgacgatc tgccggagca ggccttctat atggtcggcg gcatcgagga
    18541 agttatcgaa aaggcaaaga ggctgtcgga atagccggag gatagcacat ggcagatgaa
    18601 ctgttgttgg aaatagtcac cccggaaaaa atggccttca acggcaaggt agaagaggta
    18661 accgtaccgg gaagtgaagg agaattcggg gtcctgagag gtcacgcgtc attgctgagt
    18721 tccattcaga tcggcgaatt gagctatacc cagaacaaca aaaaggttgc ctacgcggtg
    18781 gatacggggt atgcagaggt gttggccagc aaggtgacca tcctcgtcga aggagcggag
    18841 cgctccgaca tgatcgaccg ggatcaggcc cggaaggaca aagaagaggc ggaagccaaa
    18901 ttggccaaga tggccaagga agacgccgat tacgaacggg tcaaggccgc ggtgaccaat
    18961 gcggaaaccc gtctgaaagt ggcggaaagg ggctgagcgc ccccacgggg aatagaccgt
    19021 gccgttgcgc ctcaagcagc aagtcgaaag gccggagaag ttgcttctcc ggcctttttt
    19081 tcttgcctca atccacatgg gcgatgaaga tatcaacgat ctcgaattcc cgcacccccc
    19141 ccggggcgtt tacccgggcc gtatctccga tggttttccc caccagggcc ttgcccaaag
    19201 gcgaggttac ggatatcttg ttcctgccga tatccgattc gtggggtccg accaactggt
    19261 aggtgatatt tttacctttt ttgacatcct caataatcac ggaggtaccg aagacgatcc
    19321 ggtccgtgga gagatttttg agatcgatga tctctgatgt ggccaggttg ttctccagtt
    19381 cctgaatctt gccgtggatg aaggcctgcc gttccttcgc ggcggtgtat tccgcgtttt
    19441 cagatatatc tccgtgagcc cgggccgcct cgatgtcccg gacgttttcc ggtaccgcca
    19501 cggtcttgag gtgctccaat tcccttttga tcttttcaaa acctactttg gtaatgggtg
    19561 ctttcttcat gatccacgga ctccctgccg gcataatccc gccgagtgtg atgactcatc
    19621 cctaatggat aataaccgtg ctgtcaaggc ggatttaacc ggaggaatgc caccgggcca
    19681 cgaagatcgt gaggaaggat tgccaatccg gttccatgaa gattgacgcc tgttcaatta
    19741 cttgcgccag ttttagattt gtgttaggga gcgacaagca tgtatgacaa ttaccaacgc
    19801 gatataaact atttgcgggt ttccattacc gaccgctgca atttgcgttg ccggtattgc
    19861 gttcccaagg aagggctgtc ccttatcggc cacgacgaca tcctgaggta cgaggagatc
    19921 ctccggatcg tacggatcgc cgttggactc ggcattgcca aagtccgggt aacgggtggc
    19981 gaaccgctgg tccggcgggg agtgataacc tttatgaccg ccctcaaagt cataccgggg
    20041 atcgaggacc tcagcctcac caccaatggc atcctgctgg agaggtatgc cgcggatctg
    20101 cgcgccgccg gggtgtgccg cctcaatatc agtctggact ctctgaaccg ggaaaagtac
    20161 tactacatta cccggggggg gtcgctggcc aatgccctcc ggggtatcga cgcggttttc
    20221 gagcagggtt tcgccccaat caagatcaat gtggtagcga tgaaaggttt caatgacgat
    20281 gaaatcctcg attttgccca cctgactctg agccgtccct tccaggtgcg attcatcgag
    20341 ctgatgccgg taggtcatac cggccgggat aatctcggca agtatctggc ctgcgaagag
    20401 atcatgggga caatcgagag gaactatccc ctcctgccgg ttaacggtgc ccgggaccgg
    20461 acggacggcc cggccagaat ctatcgcatc gacggcggcg ccggcgaaat aggtttcatc
    20521 accgcgatga gcagtcactt ctgtcaccag tgcaaccgtc tgcgcctgac ggcggaagga
    20581 catctacgtt cctgcctctt ttccgacgcg gagatcgact tgaagggtcc cctacgctcc
    20641 ggatgcgggg atgaccggat cgaagccctc atcaaggagg caatcgccaa taagccccgg
    20701 aaacacgaga tccgcccgga gggaaactac atccgggaat gcgggaagaa catgtcggca
    20761 atcggaggtt gaggggtttg atctttacgg aagagattgc ccgatcctat gacaactggc
    20821 gtgatacgcc ccccggacgt tatatcgaca gccgggaaaa aagcctgatt ctcgatttga
    20881 ctcagccccg gggcggggag cggcttttgg acgtgggctg cgggacgggg gatcatctct
    20941 ggcttttccg gcgcaagggg tgcgatgtaa ctggtctgga cgcttcagcc cccatgctgg
    21001 aacaggcccg ccggaaactg ggcgccaagg cggattttta cctgggagcc gcggaagatc
    21061 tccccttttc cgacaatgag ttcgacattg tcacgctgat caccgcgctg gagtttaccg
    21121 ccgatcccga acaggccctt cgcgaagcca tccgggtttg ccggggccgg gtatttatcg
    21181 gcgtcctcaa taaattttcc tgtctgggca tggccaggcg gatctccgct accttcggcc
    21241 cttccctgta caatcatgcc cgttttttca gtatcggggc gctggcggcg atgatccgcc
    21301 gccaactggc gggggttcgg gtccgatggg ggagcgtaat cttcctgccc tccggctggt
    21361 atggattcgc cgccggtctc gaggagatct tccccgtaat gaacaatccc ttcggtgctt
    21421 ttctgggttt gtcgttttcc gttaccttta cgcaggttac tcttcaggag ccaatccggg
    21481 agacgttaac catcggcgcc gaaggccggc ggcccgtgcc cggggtggtg agggtgatta
    21541 aatgaccagg gaagccatgc tttttgaacc gctggcggac ggacaggtac ggtgcctcct
    21601 ctgcgcccac cgctgtctaa tcaaggaggg ccggcggggt atctgcgggg tccgggagaa
    21661 ctgcgacgga gtgctataca gcctcgtcta tggcgcggtg atcgcggaaa acatcgaccc
    21721 catcgagaaa aagccctttt tccatctcca tccgggatcg cggtcctatt ccctggccac
    21781 ggtgggatgc aatttccggt gccggttctg ccagaatcac gagatctccc aactgccccg
    21841 ggaggcgggg acggtaccgg ggaaaaatgc tacccccgcc gagatcgtgg cccgggccgt
    21901 cgacgccgac gcccggacca ttgcctacac ctacactgag ccgacggtat tcttcgaatt
    21961 tgcctatgat atctcccgtc ttgcccggga gcgggggata aaaaacgttt ttgtcaccaa
    22021 cggctaccta agtgaagagg cgctggcgac catctctccc tatctcgatg gggccaatgt
    22081 ggacctgaaa tcctttcgag atgattttta ccgacaacaa tgcggtgccc gactccagcc
    22141 ggttctcgat accctcgtgc gcatgaaaac ggcggggata tgggtggaag tcacgaccct
    22201 gctgattccg gggctcaacg acagcgagga ggaaatcgat caaatcgccg ccttcctggc
    22261 cgggttgggt aaggaaaccc cctggcatat cagcagattc cacccccgct accaaatgaa
    22321 cgaccttcca gccactcccc gagagcggct ccagaaggcc cgggaaatcg gccgggccca
    22381 gggcctcaag tatgtttaca gtggcaatgt tcccggggat gaaggggaaa acaccttttg
    22441 tccgggatgc ggccgactcc tgatcgggcg ttacggattt tgtgtggaga gtatgatgct
    22501 caggaacgcc gcctgcatgc attgcaggac cccgctggac ggcgtctttt aaccattcca
    22561 gttcagttca tcctcgtcat aggtccgtga caggatctcc cgggcccgct cccactcccg
    22621 ctcgggaacg acgatcgtca cttcccccag gccgtcgatg gtgatggcat agatcgcccc
    22681 ggccgcctcg taacggagac gggccggaat accgtcggct tccagtcggc cgacgatgac
    22741 atgggcgata ttcagaccgc cgacccggca tacctctcgc caataaccgc cttccatctc
    22801 ttctcttttt tccatgcccc cgatcatact atatattgtg gtcgaactca agccttacat
    22861 atatgttgta ttttttcttt acaaagaggc ccccttttgg tagagtgcgc ccaatcgtag
    22921 cgcgagggac aagcaggggg caatgaaatt aaaaaatctg gaaatcaagg gatttaagtc
    22981 ctttcgggac aaggtggccc tcgactttgc ggtgggggtg agcgccgtcg tgggtcccaa
    23041 cggctgcggg aagagcaacg tggtggacgc catccgctgg gtgattgggg agcagcgggt
    23101 caagagtctc cggggcgtca ggatggagga tgtaatcttc aacggcgctc aagaggcgga
    23161 accggtcggg atggcggagg ttttcatgtc tctggcggcg gaaggtcttg cctttccgga
    23221 tccataccat gattgtgccg aggtggtaat ctccaggcgg ctcctgcgga acggtgagag
    23281 cgaatatgcc atcaacggcc tgccctgccg tctcctcgac gtccgggaat tctttatggg
    23341 cgccggggtg ggcacccgga cctattcgat cgtggaacag aacagcgtgg cttctctggt
    23401 tgaggcaaag ccggaggaac ggcggcagtt tatcgaggaa gcggcgggca tttccaaata
    23461 caagacgcgc aaggaggcgg cgcagcggaa gctggaggcc accaatcaga acatcctgcg
    23521 tttgaacgat atcctccggg aggtgaaaac acagctcaac gccctttccc ggcaggcccg
    23581 gcgagccgag cagtatcgtc agctccggaa ttcccttcgg gaggatgagc ttgcggtggc
    23641 ccgggatgcc tatggcgccc tccaccggga acaggaagaa cggtcgcggc agctccagaa
    23701 ctgtcggcag cgggaagagg agctccatac ggctctaatg ggttgcgagg catcgctggc
    23761 ggcggcccgg atcgaggttc tggaccggga agcggagcgt tcccggtgtc aggaacatat
    23821 ttatcagctc cgcacggccg tgcaggtcaa ggagcagggg attgaattta cccggggcaa
    23881 aatagccgat ctcgaggccc ggcaacaacg gctggcggag gaaatacggc agtttcagac
    23941 ccgtcatgag gaacttgggc gggaacgaga aagactcgcg gctttgcagc atatcctctc
    24001 caccgacatg gcggagatcg agagggacgt cgcggaacgg gaacgacgga tctctactct
    24061 caagggggat ttggcagccc ggcagcagcg tctggaagag aacaagagcc ggctcatcga
    24121 tgcagcggcg gagaaatcac ggttgagcaa caattgcagc cacctcctca agggtctcga
    24181 ggaaattacc cgaaaagagg aaagaaacgc cagggaaatc gccgaacagc gcggccagga
    24241 agaacgtctg cgggagaaag tggcgggcat cgagactact ttgaaggacg atctgcactg
    24301 tcttgaggag ttgcgccgta gacgggagga ggggacggtg gagtgggaag acgccgtgcg
    24361 ggagatgcag gagacggagg agcggatcga cggtctcaag gaggaattta gcagccgcac
    24421 ttcccgtctg gcctccctga aagagctgca cgacggctat gtgtggtgca atgaagccac
    24481 ccgatcgatt atgacggcca aagcggccga cagtctcaag ggcgtggatc gggatcaggt
    24541 gatcggactt gtggtcgagc agctccgtgt cccccgggag tacgagacgg cggtggaagc
    24601 cgttctggga gagaaattgc aatatgtgat tgtccggagc caggaagctg gtgttcaggc
    24661 catcgattat ttgaaggaag cagcggtggg cagaaccagt tttgtcccgc tcgcggtccg
    24721 ccacagcaca ccgggcgtac atgccgaaca cctgcgggaa acggagcgtc tcctggagcg
    24781 gattgaggtg caggacggct ttcggggcat cgccgagtac ctacttgggg acgtcctcct
    24841 catccccgac ctccggaacg gtcttgccct ctggaacagg aacggtttcc ggggcacttt
    24901 cgtgacgccg gacggagaca cgatcagtcc ccagggggtt ctgaccggcg gccgcaacgg
    24961 taacggcgcc cggagcctgc tgaagaacaa gcgggaaatt gagaccctgg aggaggagat
    25021 ccatggcctt tccgcctcct tgcagaccga tacggcccgg cggaaggaat tggccgcccg
    25081 ggtggtgcga agcgaggagg atctcgagga aatgaccgcc gccatccacg cccgggagtt
    25141 gcagtggaac agccgccaaa aggacctgga aagatttgaa gcggaatgta agcgcctgcg
    25201 gcagacccgg aaggtgctcg agttcaacgg tgaaagccag gccgaggaac gcctgctgct
    25261 cgatgaaaaa ctgcaaagcg gccgggcgga gctggagaac cagagacagc gggaggaggc
    25321 ggcgcgggag gaggcggcgg gcctgcagga ggaagtggac caactgcgcc gggatctgga
    25381 gacccgcgcc ggggaactga cggaggaaaa ggtccgccag gcgtccatgg cggagcagaa
    25441 taaatccgtt gtcaaacaat gggagcgttt acaggccgag gttgcggact tgaaagagac
    25501 gatgcggaag ggtggagacg attccctttc ctggacgcga cagctcgaag aactgcggaa
    25561 ggcggcggtc cgggacaaag cggagctcat ggaactctac gcggcgctgg cggaattgga
    25621 aaaggaactg gcggacaagg gaaatcttca tgccctggcc gagacatcac tgcgggattg
    25681 ggaagggaag cggcaggaaa cgaaggaacg gtatgagcgg atcgggcggg aagggaggaa
    25741 actggagtcc gaagtccggg agctcgacta tcagttggac ggcctgatcc gcatgtgcgc
    25801 ggagaaacat caaatcgacc tgaccgaatc gccgctgacg gagccgcccc tgagcgagga
    25861 ggagcggcgg ctccgggagg accggatcga gaaaaaccgc cggagcgccg ccgccttcgg
    25921 ggaagtgaac ctgctggcga tcaacgaata cgaacaactg caggaacggt tcgcatttct
    25981 taattcccag tcccgggacc tcaatgcgtc cctgaatacc ctgcagcaga ccattgccaa
    26041 gatcaacagg gtctcccggc agcgctttgc cgaaaccttc gaggcggtgc gcggttcctt
    26101 ccgggatgtc ttctcccgga tattcccggg cggcaagggc gagctttatc tgaccgatga
    26161 aagcgacctc ctggagaccg gggtggacat cgatatccgg atccccggca agagatccca
    26221 gaacatcagc ctgctgtccg gcggcgaaaa atccctggcg gccattgccc tgattttggc
    26281 catcattctg taccggccta cccccttcct tgttctcgac gaggttgacg cggcccttga
    26341 cgacgccaat atttccctct ttgcgaagat catccgggag attgccgtca attcccaggt
    26401 tattctcatc acccacaaca agaagaccat ggaagtagcg gagaatctct tcggcgtcac
    26461 catgcagaaa caggggatat ccaccgtggt gtccgtgtcc ctgaaccgat cctgaacctt
    26521 gcgttgacga catcgtaaaa agtccatatg cggcgttgcg cttcatcctt cgtcact
//
LOCUS       DAOSMF010000018        25849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_2415_length_25849_cov_21.917461, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000018 DAOSMF010000000
VERSION     DAOSMF010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 25849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25849
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2415_length_25849_cov_21.917461"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1808)
                     /locus_tag="PLT63_03785"
     CDS             complement(<1..1808)
                     /locus_tag="PLT63_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97877.1"
                     /translation="MIRIPATAHRFLLMLCLAGWLLTPAHLSLAAVEKTKVLIHQCQS
                     CHDETVHKQDFLASAHGKNTCVSCHGEISDLGRHMTGESAPAPVNCASCHRDIASRFK
                     KNVHYLKLNFQCQDCHHDIHAIRKSGVELEKAAVDNCCQCHDSQDYVLLGHSGSFLKG
                     NADAASCVDCHGLHDTPYYDPTLERDVAAARENYTRRCRSCHDDAKMDKRNRFSTQAV
                     TTFDDTYHGKVFNIGYPQRVAGCADCHRGHNILPRGHLLSAMHPDRMKEACGTCHQGF
                     HDRFLSFVAHPDPLDAKRHPVLYWTNAFMIALLGGTFAFFWLHTLLWWWRTYRDKCAE
                     AKAGFVEKHLLPECREEKQIQRFSMQERIMHVLLILSFFTLVMTGFPLKYYDSPWAKI
                     MINIWGGAYRAGICHRVAALVLCGLFLYTLWLSLRFLFPGGKMKGWLGRLFGPDSLFP
                     NLKDLRDVIGMFKWFFGGAEMPRFDRWTYWEKFDFFAVFWGMTAIGGSGFVLWFPELA
                     SYLMPGWMINVATVVHSEEAFLAAIFIFTIHFFNNHLVPNKFPLEPNVFTGRYRLEAM
                     RHERPLEYERLAAEGRLESLKREGPGIWLQLFSSAFG"
     gene            complement(1805..2536)
                     /locus_tag="PLT63_03790"
     CDS             complement(1805..2536)
                     /locus_tag="PLT63_03790"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOD97878.1"
                     /translation="MTMIAVLLFVLSLGIPAAVLAADEKPALVEPQAVVWMLDNREKI
                     VFINVASNLECLDTRIPSSLCMECGEGVAKAEEWPRDRETKIVFYDGNATAAPGCELV
                     QTARTRGYKKIYLLKGGLTAWKRSGFEVETIDRIPRIKGLAVKPEKLKSWLAANSSAL
                     LVDLRRPADFKSRSIAGAVNVPLASLHNTYQDLYLDRPLLLIDQDGSRSFLAAGYLHR
                     KGLEKLYRLEGGMAAWQEWNKRRKP"
     gene            complement(2577..4565)
                     /locus_tag="PLT63_03795"
     CDS             complement(2577..4565)
                     /locus_tag="PLT63_03795"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HOD97879.1"
                     /translation="MKKIDLLIIAFVMIFLGISWSGEAAAGTHRDVWLKNERGDRISP
                     GRNDSDPYSPRKTCGVCHNYSVINIGYHFQQGFDKINDRFDARRRWHLSPGMFGRWLP
                     TAAAARLAAKKNTDLRQIDLSTYDWIGGGKYEGKSKVAAVACGWCHPGGGPMEYGRDA
                     GGRADLTRNLAAGEKHSKEKFDGDYSSLWTPDGKSRFRESGVVEADCLLCHKPDYGLR
                     ERNEQLNRRNYRWAATAGAGLGRISGAVFTYSRPGAGPSDPGFREGTWNLSKRPVTAY
                     RWTDRRLFAPEGRLRGQVIRKHVADRNCLQCHGEGEAKNTGGRHDPEFDAHLRAGMTC
                     TDCHPLAGKSSSERLRHQIAKGNSTLNTVRDDLDEMGMRTCVGCHGGGRYAAPLSGTS
                     REARNPQKKHLAKFAKASFHTYLISCGGCHSVAQPGRGMAVLDMSAGQETGYTADNLE
                     GVRWLPDYDRPAKEPWKPWLARLGKQGDAGEQYTPVVPKHLQWFGERQAGGEIRPIPL
                     RYVAQAVKAVPGLSVLTVGLPGGDKGKRWTVVKDDDIIKMSRQLTGLGFREVVFVSDR
                     VYQAGTQGLKATPLPGPPVMYGVEHGVIPLAKQQAYGTKGAGEGCGDCHSDTASFFNK
                     MTIKNLRGMLKDDYPQLKDPNAVPQYESWGLKGTPSYE"
     gene            complement(4582..5622)
                     /locus_tag="PLT63_03800"
     CDS             complement(4582..5622)
                     /locus_tag="PLT63_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97880.1"
                     /translation="MYKGYLVDKDLLKKDPHFRMGCRLCHKGNEKGQGKEAAHQGLVK
                     RPSDNLRTCGMCHRQIADNYSKSLHYTTAGQRNGVMPRFSPEELKTFDKKVFEQSCRS
                     CHASCGDCHVKSPPIGGIGLGLFQRHKFVKKDESKTCAFCHGGRVYPEYTGEYGGSPD
                     VHYQKGMACMDCHKKAEFHGDGTAYTTKQEVRDRPSCRNCHKLGQEGKLTARVSHKRH
                     KDKVSCYGCHTATSYRQCQDCHAGKGATSQPVLLLGLNPRDRKTLTTLRLIPTVRETF
                     VKAGIKMENFDALPNYWDSPVHNIRKRTERTRNCDVCHVERARFLDVETMIRGGSKEN
                     QRLIVIPKPINR"
     gene            complement(5656..5823)
                     /locus_tag="PLT63_03805"
     CDS             complement(5656..5823)
                     /locus_tag="PLT63_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97881.1"
                     /translation="MKSTVTLYVLLFFFSLSVAVIPVQASSCVQCHTSDAVMKSLYKQ
                     PAVTAGEEGEG"
     gene            6420..6833
                     /locus_tag="PLT63_03810"
     CDS             6420..6833
                     /locus_tag="PLT63_03810"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOD97882.1"
                     /translation="MKRFKYPLSIAAALLGVFLAAAVFAGHPEIPRISVEELKQLVDA
                     KAEVIILDAQLKEIYDRGHIRGAVSFPWKAEVTDADVPMFAKDKLIVTYCDCGPGESD
                     SADLAFQLQGLGFTNVKVLADPAVRGWKKAGYPME"
     gene            6842..7333
                     /locus_tag="PLT63_03815"
     CDS             6842..7333
                     /locus_tag="PLT63_03815"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HOD97883.1"
                     /translation="MTKNIPMLLLILVGAAGLAFFSAVPVSHSQPEPEGYAGANACAM
                     CHSELTAGWKTTRHARAFDTLKKKSQEKLPACVKCHVVGFEKPGGYVDQELTPELAGV
                     QCESCHGPAAAHAANPANKKGLIDPGEKTCRECHTPGQDPKFDYAGKKRFVHGESGKG
                     GVK"
     gene            7339..7551
                     /locus_tag="PLT63_03820"
     CDS             7339..7551
                     /locus_tag="PLT63_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97884.1"
                     /translation="MNKRPLSIRGLLIALALFVMTGAAFAADSLRISPVRIECGLIEE
                     GTPATMVAVVENISGKEVRITGIRTN"
     gene            7567..8058
                     /locus_tag="PLT63_03825"
     CDS             7567..8058
                     /locus_tag="PLT63_03825"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOD97885.1"
                     /translation="MLGQHTLQPGERTELKAVFDTKNAPGPFEKITTIQTDAPGQEQI
                     ELVMAGTVKEAPGPKIAVLPRRTDLGAVKPGEAKQVKITVTNPGELPLTITAVKVRPG
                     GAIAAAAGALPAQIAAGQKAELMLIISIGRPGTFTERISLQSNAKNAPKSGFVLMVSG
                     RAE"
     gene            8141..9895
                     /locus_tag="PLT63_03830"
     CDS             8141..9895
                     /locus_tag="PLT63_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_273701391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:8540..8542,aa:Sec)
                     /transl_table=11
                     /product="UGSC family (seleno)protein"
                     /protein_id="HOD97886.1"
                     /translation="MKTRTKFSVMWLLALGVFILTPLGAYAKEAIQSWELVNPAGVVE
                     VKYFKPAPRLTSLDGKTIVLRWNEKHNGDLFLNRIAELLQKEVPTAKVIKLYEVDKST
                     IKISGSAKESNRIADVIKGLKADIVIATQADUGSCTSWLVVDQSEVEKRGIPTVTLAS
                     NQFLSLAKSTVKSTGIPEMAFVEFEHPMGMIPAGEVRAKANKAFPEIMRMATKWQPTT
                     AVAAAAKPPYPAERIRFKGTYASLNKMFYEKGWSLGLPIIPPTPEAVKAMLKGTKHKP
                     GEVVWVVPPRQGQLTVELVATIGVMAGCKPEHMPLLLTIVKVLKEPDFDWRGNSTTTA
                     PTAPLLVINGPVVKELGISASTGALGGQMPVNISLGYFVNLVGDIVGGSVPPDSDKST
                     LGSREDLVAMVVAENESRNPWKQSYAVEHGFKATDSVVTGFGAYMGTNNTDHNSVKGK
                     DLLNTFAVGLAGASCGITSCYTRLDQPSTWQNGVKFAFLLVGPEHADTMYRDFPTKLD
                     VQNYLVKKAVLPYSGYSPGQCIVPKDFGPYDENTMFPRFTQPEQIKIVVTGGSGKQSQ
                     IWIPFLTDNRPVSAVMEK"
     gene            9966..11258
                     /locus_tag="PLT63_03835"
     CDS             9966..11258
                     /locus_tag="PLT63_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97887.1"
                     /translation="MKRSKKKSFWGAVAAGLLILCLSAPAAAAPRTVEVYNPAGQQPD
                     GVYVDTILKKIMDETGVKRPLLAGKFTGVKKERIGIRHDEFSEVSEWFYLRGWTDGLP
                     IVPPTERRVKEMLKGTDLPADFLVANLDPMGGQATVEKIAVNAVMAGCRPEYMPVLIA
                     AVELAARPEFDLKGLATTTNPDAPLLILSGPIVEQLDVNAGTNTLGRGWQANASISRA
                     LHLIIQNIGGSWPGVTDMSTIGQPGDFVMMLAENSAANPWGPLHMELGHPRAANVLAV
                     MAAEGTHSILGIGQSDEGFLKLVAAHLAGLERPYRETMLLIIAQDTAKMLADKGWTRE
                     RMIAYITDRAKIPFRLYKERYLDSSALRKGMPDWVFRVQDPDAMIPSPLIGKLLILVA
                     GGTGEKSTLIPGWYDAGIMSKEIRLPANWDELLPKKNP"
     gene            complement(11366..12124)
                     /locus_tag="PLT63_03840"
     CDS             complement(11366..12124)
                     /locus_tag="PLT63_03840"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97888.1"
                     /translation="MASANTASEEKNIWKVVRVAGENSDVNSLFLEGKDEKFTARKAG
                     QFVSIRIPAAEGWSEPHPFTISAAPEDNLLRLTIKKEGVFTSAIPSLTPGTPVKVMGP
                     LGVFCQGIDDRPEIVMLAGGVGITPFLSVLRHFRNCGAKNRVILFWSNKTLADVFCRE
                     EIAAMTGELPLNVVHCLSREDDVQSYFDARIPGVKYEQGRLSAEIMKKHGVKENAACY
                     VCGPPPMMDTALKELESLGVDPAQVEQEQFVWKK"
     gene            complement(12492..13502)
                     /gene="galE"
                     /locus_tag="PLT63_03845"
     CDS             complement(12492..13502)
                     /gene="galE"
                     /locus_tag="PLT63_03845"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004220743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOD97889.1"
                     /translation="MKILVTGGAGYIGSHTCVELLRAGHDVVILDNLSNSKVSVLSRI
                     ETITGRRPAFFHGDCRDRSFLRAVFEVHPGDAVIHFAGLKAVGESVTQPLFYYENNVG
                     STLALCEVMADHGLKRLVFSSSATVYGDPATVPIREDFPLGPTNPYGRTKMMIEQILQ
                     DLKTSDPEWHVILLRYFNPVGAHESGLMGEDPAGIPNNLMPYISQVAVGKLDRLLVFG
                     NDYPTPDGTGVRDYIHVVDLAQGHLKALERLAEAGDFGIYNLGTGRGYSVLEMIRAFE
                     KAAGRPVPYEIVGRRSGDIAVCYADPGLAKKDLNWRAVRGLEEMCADAWRWQSRHPCG
                     YP"
     gene            13687..14148
                     /locus_tag="PLT63_03850"
     CDS             13687..14148
                     /locus_tag="PLT63_03850"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOD97890.1"
                     /translation="MGYIKIKFKNERGKFEQEIQDVLDDMFHISGPAFTLGEHTWRPH
                     ADIYETSDAIVVILDLAGVVREDIHLEVSRKTIKISGKRDQSYFSGTTRYHLAEISYG
                     YFERQLALHAPVDSETVQATYANGLLEIRLIKIPPDRERRIPVLGPTGSES"
     gene            14178..16526
                     /gene="lon"
                     /locus_tag="PLT63_03855"
     CDS             14178..16526
                     /gene="lon"
                     /locus_tag="PLT63_03855"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOD97891.1"
                     /translation="MTEEKTAQDLKVIHIPETLPVLPLFNMIVFPKMMLPMEVFGDQS
                     LQLVDEAMAKDRIIGLVLSRKSPPETTYDPEDFHAVGTSAVILKMAKAAEKKAQLLVQ
                     GIERFRIREFIPGKPYLQARIESIQEQEGKDIEIEALMANLVSLFERIVKLTPFLPQE
                     FGAMAKTITDPGVLADLIASIINVSAEEKQGILEATATKKRLREVTRLVNHQVEILEL
                     GNKIQSQVKDDMDKSQRDYYLRQQLTAIKKELGEEDETKVEVEEYREKLAGKDLPEEA
                     RKEAERELQRLGRMHPSSAEYNVVSTYLDWILTLPWREGTEDNLDIKKARKVLDDDHY
                     GLEKPKKRIIEYLAVRKLKPDTKGPILCFVGPPGTGKTSLGQSIARSLGRKFVRIALG
                     GVRDEAEIRGHRRTYIGALPGRIIQSIRRAETNNPVFMLDEIDKLGSDFRGDPSSALL
                     EVLDPAQNFSFMDHYLDLPFDLSHVMFITTANILDTIPPALRDRLEIIELVSYTVEEK
                     LHIAQRYLLPRQIRENGLQPSQITFTKGALRVIIGSYTREAGVRNLEREIAAVCRGVA
                     SQVAVGDIERAKIRRQDIHRYLGPERITTETTARTSKPGVVMGLAWTPTGGDLLFIEA
                     TAMKGKKGLTLTGQLGDVMKESASAALSFIRANAESLGIARDFFEDVDIHIHVPSGAI
                     PKDGPSAGVTILTALTSLLTNKRIKKDLAMTGEITLRGLVLPVGGIKEKVLAAHRAGV
                     KTIILPKWNKKDLGDIPAKIRKDIAFHLVDDMLEVLRIALEK"
     gene            16714..17382
                     /locus_tag="PLT63_03860"
     CDS             16714..17382
                     /locus_tag="PLT63_03860"
                     /inference="COORDINATES: protein motif:HMM:NF037007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOD97892.1"
                     /translation="MMTDKQSLTTARILDAATDIFAEAGFNGARMDEIARRAGVNKAM
                     IYYHIGAKEALYAEVLHGVIGHVADSISACIETGATPEAKLRAYIRGFVDAVEGNPRM
                     GPILLREIASGGQNLPDLVIQDFARIIALITAILDEGRERGVFYPVTPFLIHMTVVGS
                     LILYKTSGPIRSRRENIPAAVKALDQQITPRVTVEVENLILRGIKKPPRPAAEVTNDD
                     DDPR"
     gene            17363..18877
                     /locus_tag="PLT63_03865"
     CDS             17363..18877
                     /locus_tag="PLT63_03865"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOD97893.1"
                     /translation="MMMTPVEKSGRRSWPPAVSNQPVTPPPGTAIPARPGVPRFGLLA
                     VVALLLAFLPFSPVLGQSPAVRPEMTAATPSTAPCRFTLAESVAMALKQSMVVQAAKE
                     GVKGAEAQKKEAFTGFLPKLSTSYSYTRLNDAPWTNMPPMTISSPILPAPLTTPGGPI
                     TVGTQDNYSWALEFRQPLFAGGSILANYQANRKGEDIARVDETAAAQNIVEEVKSAYF
                     QILKAQKILGVARQAVEQLKSHRDEARHFFDVGLIPKNDLLQSEVQLANGEHNLVRAE
                     NSLEIVKSRFNTLLRRPIDAPVEVEDILAYRPFPKAVEECLQTAYSQRPEIKAHALKV
                     EQAREVVKAARGNFFPTVSAVGHYERFGDTGSLQGSTYKSMENWYLMARADWTFWEWG
                     KTKNQVDVHRARENQAGSMLILERDKVTLEVKAAYILVRESEKQVFVAQKSIDQAEEN
                     FRINNERYREQVATSTDVLDAQTLLTKAKSDYFTALGDYFINQARLERAMGVSQ"
     gene            18874..19884
                     /locus_tag="PLT63_03870"
     CDS             18874..19884
                     /locus_tag="PLT63_03870"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOD97894.1"
                     /translation="MKKRVVLAVFVALLVAVGGYVYYGQWKNKHRETYYSGVIEAKDA
                     KLAFQAGGRVIAVHAREGQAVTKEEVLVELDAAEFQSRCDQARAALARAVKGQEQAET
                     SLQVYQKVLPADVIRAEAAVTSARRVMEEARRNKDRYDKLYQRKVVAEKEWDAVRLHY
                     DTSVSRLAEGKAALSQAVSNLKKIDIARREIEAATAQVRLARASMEQAEIQAAYTKLR
                     APFAGVITSRNVEPGEVVSPSREVFNLADLSRVDLKIFVEETAIGKVRPGQAVEVKID
                     TFPNHVFPGVVSFVSPEGEFTPKIIQTQKERVKLVYLVKVSIPNPDLALKTGMPADAW
                     LK"
     gene            19995..>20762
                     /locus_tag="PLT63_03875"
     CDS             19995..>20762
                     /locus_tag="PLT63_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD97895.1"
                     /translation="MTAHPDSFVSIEHVSMRFGAVEAVHDVTLKVTAGTIFGLVGSDG
                     AGKSTLLRMIATMIKPAAGRITVDGHDVAAEKNRVKDLIGYMPQRFGLYADLTVDENL
                     RFFMDIFGIPRPERKRRREKYLGFSNLLPFADRPAGALSGGMKQKLGLACVLIHEPRL
                     LILDEPTNGVDPVSRQEFWEILGEMRREGMTILVSTAYLDEGEKCGGLALMHKARIID
                     VATPAAIRTAFPSLEAAMISRLQEADAEIGKDAFFAHP"
     assembly_gap    20763..20862
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            20884..21606
                     /locus_tag="PLT63_03880"
     CDS             20884..21606
                     /locus_tag="PLT63_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD97896.1"
                     /translation="MRVKDLEKRFGKFTAVNRVTFSVRKGEIFGFLGANGSGKSTTIR
                     MLCGILTPTAGEGMVAGYDIFREPEEIKGAIGYMSQKFSLYEDLSPGENIRFYLGVYN
                     VPPYRWEERTAWILETTQLGDVRNRLTRELPQGWRQRLALGCALLHEPEILFLDEPTS
                     GVDPVTRQRFWDLIRRLAAGGITIFVTTHYMDEARHCERIVMLNQGAIVAAGSPEEII
                     ARSCPDREDADLNDAFITLMKN"
     gene            21687..22820
                     /locus_tag="PLT63_03885"
     CDS             21687..22820
                     /locus_tag="PLT63_03885"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOD97897.1"
                     /translation="MNLRIIAAITRKEFYHLIRDYRSLYLAFAIPLILILLFGYALSL
                     DVDNVRTVVVDHDNTPRSRDLIRRLDASPYFHVAARLGNSEDAAAWLDHGRTILAVVI
                     PPDWTRNIQADREEPLQILLDGSDPNFANISAGYITAFLERENTAALHNYLNRQGQEQ
                     LRPPLEGRIRIWFNEDLESRNFIVPGIIAVIIMIVGAMLTSLVIAREYENGTMETIKS
                     LPVSAGDFIVGKAIPYFFITLADVLIAILMGQVLFGIVMKADFWLMISACALYLAVAI
                     TLGLFISAAVKSQLVANQIAPIVTFLPSMLLSDFVFPIMNMPAAIQVITYIVPARYFI
                     DILKGVYMKNADFTLLWPNFVVLFIMAAGLGALNLIVLKREGM"
     gene            22822..23943
                     /locus_tag="PLT63_03890"
     CDS             22822..23943
                     /locus_tag="PLT63_03890"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOD97898.1"
                     /translation="MCRLRIRELVRKEFIQIFRDKRNRPILIVAPLIQILIFGYVVNF
                     DINIIHVALLDQSRTLESRKIVDAFSANHLFQIDNIVHNSQEMEEILIRGKADIGLKI
                     GPDLARKIRRGETAEVQILADGTVSNMAAIRVSYVMTVMERLNRQFLQDRFPAQLSYG
                     RIDGRLRTWYNPNLYSRHFFVPGIVAFIVMLISLLFTSMAVIKEKEGGTMEQLIVTPM
                     KPYELIMGKTIPYIVISLIQMAGVLLFAVYLFDLPIRGSLSLFFLAICLFLMSTLGIG
                     LFISTISATQQQAMMSTFFFLQPSFLLSGFVFPIANMPVVVQWLTYLNPFRYILVIIR
                     GIFLKGVGMDVLWPQYLSLTILGLAVFTAAVLRFHKRLD"
     gene            24172..25191
                     /locus_tag="PLT63_03895"
     CDS             24172..25191
                     /locus_tag="PLT63_03895"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOD97899.1"
                     /translation="MKGTIYRQCVGLGNESVSTLKRLLHEMPHPFRKGDNIGIKLHWG
                     EKGNHTFLPPVFAREIVVWLRELGATPFVFDTSVLYSGSRRNGSDSLKTAAAHGYSEA
                     YLDCPVVVADGLDGRAVIDIPAGYRHFETVQVADIFKRASGFVILSHFKGHMVSGFGG
                     AIKNISMGWASRAQKQRMHSDARPRLEKNSCSQCGLCVDICPSGAAQIAADGYPDYSQ
                     ELCIGCAQCIGLCPELALEICWNTDINVFQEKLVETAAAIWKQIQGRAVLVNAMLNIA
                     SECDCLPGQHPIIAPDAGFVGGYNPVSVDVESINRIGKEPFIKAHPHVDWKHQFSYGR
                     EIGFA"
     gene            complement(25603..>25849)
                     /locus_tag="PLT63_03900"
     CDS             complement(25603..>25849)
                     /locus_tag="PLT63_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97900.1"
                     /translation="FYDAVPNGTFFALFTMSSILPDNAFLVQENLTLSMNMQPLRTCS
                     RCRSRRGAVLAPLGDDREFSRFSIFRTLITSLPQWPT"
BASE COUNT         5586 a         7739 c         7325 g         5099 t
ORIGIN      
        1 ccgaaggccg aagaaaacag ttgcagccag atgcccgggc cttctctctt cagggattcc
       61 agacgccctt ccgccgccag gcgctcgtat tcaaggggac gctcgtgacg catggcctcc
      121 aggcgatagc ggccggtgaa cacattcggc tccaggggaa acttgttggg aacgagatgg
      181 ttgttgaaga aatggatagt aaagatgaag atcgcggcca gaaaagcctc ttcggagtgc
      241 actaccgtgg ccacattgat catccagccg ggcatgagat aggaggcgag ttccggaaac
      301 caaaggacga acccggaacc gccgatggca gtcatgcccc agaagacggc aaaaaaatca
      361 aatttttccc agtaggtcca acggtcgaac cgcggcatct ccgccccgcc gaaaaaccat
      421 ttgaacatgc cgatgacgtc ccgaagatcc ttcagatttg ggaagaggga atcgggaccg
      481 aaaagccgcc ccaaccaccc tttcatcttc cctcccggga agagaaaccg cagggacagc
      541 cacagggtat aaaggaaaag accacagagg accagggccg ccacgcggtg gcagatgccg
      601 gcccgatagg cgcctcccca gatattgatc atgattttcg cccaggggga gtcgtaatac
      661 ttcaacggga atcccgtcat caccagggta aagaaggaga gaatcaacag gacgtgcatg
      721 atcctttcct gcatggagaa acgctggatc tgcttttcct cccggcactc gggcaggagg
      781 tgcttttcca cgaaccccgc tttggcctcc gcgcacttgt cgcgataagt acgccaccac
      841 cagagaagcg tatggagcca gaagaaggcg aaggtgcctc ccagcaaggc aatcatgaag
      901 gcattggtcc agtagagcac ggggtgccgc ttggcatcca gtggatcggg atgggcaaca
      961 aaggaaagaa aacggtcgtg gaagccttgg tggcaagtgc cgcaggcctc cttcatccgg
     1021 tcgggatgca tggcggacag cagatgaccc cgaggcagga tgttgtggcc gcggtgacag
     1081 tcggcgcagc cggccacccg ctggggataa ccgatgttga agaccttgcc gtgataggtg
     1141 tcgtcgaagg tggttacggc ctgtgtggaa aagcggttgc gcttgtccat cttcgcgtcg
     1201 tcatgacagg aacggcaccg gcgggtgtaa ttttcccggg cagcggcaac atccctctcc
     1261 agggtgggat cgtaataagg ggtgtcgtgc agtccgtgac agtccacgca tgaggcggca
     1321 tcggcgtttc ctttgagaaa agaaccgctg tgccccaaaa gcacataatc ctgggaatca
     1381 tggcactgac agcaattatc caccgccgcc ttctcgagct ccaccccgga tttccggatg
     1441 gcgtggatat cgtgatggca atcctggcac tggaaattca attttagata atggacattc
     1501 ttcttgaaac gggaggcgat gtcacggtgg cagctcgcac aattcaccgg cgccggcgcg
     1561 ctttcgcccg tcatatggcg acccaggtcg ctgatttctc catgacagct cacacaggtg
     1621 ttcttaccgt gtgccgaagc gagaaagtcc tgtttatgaa cagtttcatc atgacaggac
     1681 tggcattggt ggatcagcac ctttgtcttc tcaacagccg ccagggagag atgggcgggg
     1741 gtgagcaacc agcctgccag gcagagcatc aacaggaatc ggtgggcggt ggccgggatc
     1801 ctgatcatgg tttcctccgt ttgttccatt cctgccacgc cgccatccct ccctccagcc
     1861 ggtacagctt ttccagaccc ttgcggtgca gatagcccgc ggcgagaaaa gaccggctgc
     1921 cgtcctgatc gatgaggagc agtgggcggt ccaaatagag gtcctgatag gtattgtgca
     1981 gagaggccag cggcacgttg acggccccgg caatgcttct cgatttgaaa tccgcgggcc
     2041 ggcgaagatc gaccagcagt gccgaactgt tggcggccag ccagctcttc agcttttccg
     2101 gttttacggc cagacctttt atccgcggga tccggtcgat ggtctctacc tcgaatccgg
     2161 atcttttcca ggccgtaagc cctcccttca ggagatagat ctttttgtaa cccctggtgc
     2221 gggcggtctg caccagttca catcccggag cggcggtggc attgccgtcg taaaagacta
     2281 tcttcgtctc cctgtcccgg ggccactctt ccgccttcgc caccccctct ccgcactcca
     2341 tgcagagcga agagggaata cgggtgtcca ggcattccaa attacttgcc acattgatga
     2401 acacgatctt ctcgcggttg tccagcatcc agaccacggc ctgtggttcg acgagcgccg
     2461 gcttttcgtc cgcagcaagg acggcggccg gtattcccag gctcaggaca aacaacagta
     2521 ccgcaatcat tgtcattccg ttatatttcc tcatgacgcg caccggttat tctcccttac
     2581 tcataagacg gcgtgccctt caacccccac gactcatact ggggcacggc attgggatct
     2641 ttgagctggg gataatcgtc cttgagcatt ccccggagat tctttatggt cattttgttg
     2701 aaaaatgacg ccgtatcgct gtggcagtcg ccgcatccct cgccggcgcc tttcgtcccg
     2761 tatgcctgtt gcttcgccag ggggatgacg ccgtgttcga cgccgtacat caccggcggc
     2821 cccggcagtg gtgtcgcctt caacccctgg gtaccggcct gataaacccg gtccgaaacg
     2881 aacaccacct cccggaaacc caggccggtc aactggcgcg acatcttaat aatatcgtca
     2941 tccttcacca ctgtccaacg tttcccctta tccccccccg ggagacccac cgtcagcacc
     3001 gacaaacccg gaacggcttt caccgcctga gccacatacc tcaggggaat gggacggatc
     3061 tcgccaccgg cctgtctttc tccaaaccat tgcaggtgtt tgggaacgac gggggtatat
     3121 tgctcccccg catcaccctg ttttcccaat ctggccagcc agggtttcca gggttccttt
     3181 gccgggcggt cgtaatcggg aagccagcgg actccctcca gattgtcggc ggtatagccg
     3241 gtttcctgtc cggcgctcat gtccaggacc gccatgcccc ttcccggctg agccacgctg
     3301 tgacagccac cgcaacttat gagataggta tggaaggacg ccttggcaaa tttggcgaga
     3361 tgcttcttct ggggatttct ggcctcccgg gaggtcccgg aaagcggcgc cgcataacgg
     3421 ccgccgccat ggcagccgac acaagttctc atgcccattt catcaagatc atcccggacg
     3481 gtattgaggg tggagtttcc cttggcaatc tgatggcgga gacgttcact tgaggatttt
     3541 cccgccaggg ggtgacagtc cgtacacgtc atgccggcgc gaagatgagc gtcaaattcc
     3601 ggatcgtggc gtccgccggt attcttggcc tctccctcgc catgacactg cagacaattc
     3661 cggtcggcga catgcttcct gatcacctga ccccgcagac gaccctccgg ggcgaacagg
     3721 cgccggtccg tccaccggta agccgtgaca ggccgtttgg agagattcca cgtgccctcc
     3781 cggaaaccgg gatcactggg tcccgctccc ggccggctgt aagtgaaaac agcgccactg
     3841 attctgccga ggccggcgcc ggccgtggca gcccagcggt agttgcgccg gttgagctgc
     3901 tcattccgct cgcgtaaacc gtaatccggc ttgtggcaga ggagacaatc cgcctccacc
     3961 accccgcttt cccggaaccg gctctttccg tccggagtcc agagagagct gtaatcaccg
     4021 tcgaattttt ctttgctgtg tttttcgccc gcagcgagat tccgggtaag gtccgcccgt
     4081 cctccggcgt ctcggccgta ttccatgggt ccgccgccgg gatggcacca gccgcaggcc
     4141 acggcggcaa ccttgctttt cccttcatat ttgccgccac cgatccagtc gtaggtggat
     4201 agatctatct gccggagatc cgtgtttttt ttggccgcca gccgggcggc ggcagccgtg
     4261 gggagccagc gaccgaacat ccccggcgaa agatgccagc gccgcctggc gtcgaagcga
     4321 tcgtttatct tgtcgaaacc ctgctggaaa tggtatccta tattaataac tgaataatta
     4381 tggcaaacac cgcaggtttt tctgggactg taggggtcgg agtcgttacg acccgggctg
     4441 atcctgtccc cccgttcatt tttcagccag acatcccgat gcgtcccagc cgccgcctct
     4501 cctgaccagg agatacccag gaagatcatt acgaaggcta taatgaggag gtcaattttt
     4561 ttcatccgga tgagccatac attatctgtt aatgggcttc gggatcacga tcagacgctg
     4621 attctctttg gagccgcctc tgatcatcgt ttccacgtca agaaaacggg cccgttcgac
     4681 gtggcagaca tcgcaattgc gggtccgctc ggtacgtttg cggatgttgt gcaccggcga
     4741 gtcccagtaa ttgggcaggg cgtcgaaatt ctccatctta atcccagcct tgacaaaggt
     4801 ttcccggaca gtggggatga gacgcagggt ggtcagggtc ttccggtccc tgggattcag
     4861 tcccaaaagc aacaccggct gggaggtggc gcccttgccg gcatggcagt cctggcactg
     4921 gcgatatgag gtcgcggtgt gacaaccgta gcagctcacc ttgtctttgt ggcgcttgtg
     4981 ggatacccgg gccgtcaatt tcccctcctg acccagttta tgacagttac gacatgaggg
     5041 acgatccctg acctcctgct ttgtcgtata ggctgtaccg tcgccgtgga attccgcttt
     5101 tttatggcaa tccatgcagg ccatgccctt ctggtaatgg acgtcgggac tgccgccgta
     5161 ttctcccgta tattcaggat aaacccggcc gccatgacag aaggcgcaag ttttgctctc
     5221 atccttcttc acaaatttat gccgttgaaa cagccccaga ccgatcccgc cgatgggtgg
     5281 ggatttcacg tgacagtcgc cgcaggaggc gtgacaactc cggcaggatt gctcgaacac
     5341 cttcttgtcg aaggttttga gttcctccgg ggaaaacctc ggcatgacgc cgtttctctg
     5401 tccggcagta gtgtaatgaa gtgattttga atagttgtct gctatctgac ggtgacacat
     5461 cccacaggtg cgcagattat cggagggtcg tttgaccagt ccctggtgtg ccgcttcctt
     5521 gccctgccct ttttcattgc ctttatggca caaccggcaa cccatgcgga aatggggatc
     5581 cttcttgagc agatccttat ccaccagata gcccttgtac atgtcttccg ccctaactgg
     5641 cggaggcgct cccgctcacc cctccccctc ttccccggcg gtgaccgccg gctgtttgta
     5701 gagggacttc atcaccgcgt cgctggtgtg gcactggacg caactgctgg cctgaaccgg
     5761 aatgacggcg acggacaggg aaaaaaagaa gagcagcaca tacagggtaa ctgttgattt
     5821 catctatcct cccatgtctt ctttaatatt ctttttacat atagcacggt aaaaagtcaa
     5881 agtaattccg ggttacggac ggatttttgc cgcccgttga catgatattg ccattgccgg
     5941 taactgattc ggaaccggca tcggtatgga gaacctcgcc cgctacatca tccgggcgcc
     6001 tttctcacag ggtgcagtac ctggatcagg aggggacgat tacatcgaag cataacgcag
     6061 cgaaaaaaga ggcgtgccgg aaaggtctgt ccaaaaaaca cctcaaaatg cctaccagac
     6121 tggcaagtcg cacagaaaga gagcagatga cgccagcaga ccgccgatcc acttgctgtg
     6181 tatccgcccc tggttttccg atggaaatat cccttgacac gaaaataccg cgctcctata
     6241 ctctaaccca tcagaagtaa tgacttatca aaccgctttt ccacagggtc gcttccgggg
     6301 tatccgccct atgtattttt catcatttgt cttatggaca aatggagttc aattcattat
     6361 cataaccatc aagaaacttt caaagacgtc aaaatgcagc gagaaggggg ggaaagatta
     6421 tgaaaagatt caagtatcca ctgtccatcg ccgcggcgct gctgggcgtg tttctggccg
     6481 ccgccgtttt tgccgggcat cccgaaatac cgcgcatttc tgtcgaagaa ctgaagcagc
     6541 tcgtcgacgc caaagccgag gttatcattc tggatgccca actgaaggag atctatgacc
     6601 gggggcatat ccggggggcg gtgtcgtttc cctggaaagc cgaggtcacc gatgccgatg
     6661 tgccgatgtt tgcgaaggat aagctgatcg tcacctactg tgattgcggc cccggcgaat
     6721 cggacagcgc cgatctggcc tttcagttgc agggcctcgg gttcacgaat gtgaaggtat
     6781 tggccgatcc tgccgtcagg ggttggaaaa aggccggcta tcccatggag tgaggtgcga
     6841 aatgacgaaa aatatcccca tgctattact gatcttggtg ggcgccgccg gcctcgcctt
     6901 cttcagcgcc gtaccggtca gccattccca gcctgaaccg gagggttatg ccggagcaaa
     6961 cgcttgcgcg atgtgtcaca gcgagttgac ggccggctgg aagacaaccc gccacgcccg
     7021 ggcatttgac accctgaaaa agaaatccca ggaaaaactc ccggcttgcg tcaagtgcca
     7081 cgtggtgggc ttcgaaaaac ccgggggata tgtggatcag gagttgaccc ccgagctggc
     7141 cggggtgcaa tgcgagtcct gtcacggacc ggcggccgcc catgccgcaa acccggctaa
     7201 caagaaaggt ctgatcgatc ccggcgaaaa gacctgtcgg gaatgtcaca ccccggggca
     7261 ggatccgaag ttcgactatg ccggcaagaa acgcttcgtc cacggcgaaa gcgggaaagg
     7321 aggggttaaa taaacgaaat gaataaaaga ccgttgtcaa tccggggact gctgattgcc
     7381 ctggcgctgt tcgtcatgac cggcgccgcc tttgccgccg acagcctgcg catctcgccc
     7441 gttcggatcg aatgcggctt gattgaggaa gggacgccgg cgaccatggt ggccgtcgtg
     7501 gagaatatca gcggcaaaga ggtgcggatc accggcatca ggaccaattg agcatgcact
     7561 gagagtgtgc tgggacagca caccttgcag ccgggagaaa gaacggagct gaaggccgtt
     7621 ttcgacacca aaaacgcccc cggccctttt gaaaaaatca ccaccatcca gacggacgcc
     7681 cccggacagg aacagatcga actggtcatg gccggcacgg tcaaagaagc gccggggccg
     7741 aagatcgcgg tattgccccg ccgaacggac ctgggcgccg tcaagccggg ggaagcgaag
     7801 caggtgaaaa tcacggtgac caatccggga gaactgccgc taacaatcac cgcggtgaaa
     7861 gtcaggccgg gcggcgccat tgccgcagcg gccggagcgt tgccggcgca gatcgccgcg
     7921 ggacagaaag ccgagctgat gctgatcatt tccatcggca gacccggaac gtttacggag
     7981 cgcatttctc tgcaatcaaa cgccaagaac gcccccaaaa gcggttttgt gctgatggtt
     8041 tccggccggg cggagtgagt gcggccggaa tgattcaaga agcggaaaga agaagacttt
     8101 tcaacaaaac gtggaaaaga aaaaaacaag gagaaaggtt atgaaaacac gaacaaaatt
     8161 cagcgtcatg tggctgctgg cgctcggcgt ctttatcctg acgccgctcg gcgcctatgc
     8221 caaagaggcg atccagtcct gggagctcgt taatcccgcc ggggtcgtcg aggtaaagta
     8281 cttcaaaccg gcgccgcgcc tcacctctct ggacggtaag acgatcgtcc tgagatggaa
     8341 cgagaagcat aacggcgatt tatttctcaa ccggattgcc gagcttctcc agaaagaagt
     8401 tcccaccgcc aaagtgatca aactttatga agtggacaaa tccacaatca aaatcagcgg
     8461 atcggccaag gagtccaacc ggatcgccga cgtgatcaag ggtctcaagg cggacatcgt
     8521 catcgccacc caggccgatt gaggatcctg cacctcatgg ctggtggtcg accagtccga
     8581 agtagaaaaa cgcggcatcc ccacagttac cctggcgtca aaccaatttc tttcccttgc
     8641 taaaagcacc gtcaaaagca ccggcattcc cgagatggcc tttgtggaat tcgaacatcc
     8701 gatggggatg attcccgccg gagaggtcag agcgaaggcc aataaggcct tcccggaaat
     8761 catgagaatg gctaccaaat ggcagccgac gactgccgtg gccgcggctg cgaaaccgcc
     8821 ctatccggcg gaacggatca ggttcaaagg cacctatgcc tctctgaaca agatgtttta
     8881 tgaaaaaggc tggtctctgg gactgccgat aatccccccg acccccgagg ccgtaaaggc
     8941 catgttgaag gggacaaagc ataagcccgg cgaagtcgtc tgggtcgtgc cgccgcggca
     9001 gggccagttg accgttgagc ttgtggcgac catcggcgtc atggccggct gcaaacccga
     9061 gcatatgccg ctgcttctca ccatcgtcaa ggtcctcaag gaaccggact tcgattggcg
     9121 cggcaacagc acgacgacag cacccacggc gccgctgctc gtcatcaacg gaccggtcgt
     9181 caaggaactg ggcatctccg cttccacggg cgccctgggc ggacagatgc cggttaatat
     9241 ttcccttggc tattttgtca atctggtcgg cgatattgtc ggcggctccg tgccgcctga
     9301 ttccgacaag agcaccctgg gcagcaggga ggatctggtg gccatggtgg ttgcggaaaa
     9361 cgagagccgg aatccctgga agcaatccta tgccgtagag cacggattca aggcaaccga
     9421 cagcgtcgtg acgggtttcg gcgcctatat ggggacgaac aacacggatc acaacagcgt
     9481 caaggggaag gatctcctca acacttttgc cgtgggtctg gccggcgcat cctgcggcat
     9541 cacctcatgc tataccaggc tggatcagcc gtccacctgg cagaacggcg tgaagtttgc
     9601 tttcctcctg gtcggtcccg agcatgccga caccatgtac cgcgactttc cgacgaagct
     9661 ggatgtccag aactacctcg tcaagaaagc ggtcctgccg tactcggggt attcaccggg
     9721 acagtgcatc gtgcccaagg acttcggccc ttacgatgaa aacaccatgt ttccgcggtt
     9781 tacacaaccg gagcagatca aaatcgtcgt gacgggcggg tcggggaagc aatcccagat
     9841 atggattccc ttcctgacgg acaaccggcc ggtatcggcg gttatggaga agtagcttca
     9901 cttgtggcat taaacacgga gggggaccat tcgtccccct ccgttattct ctcaggagcg
     9961 ggaaaatgaa gcggagcaaa aaaaaatcct tctggggcgc tgttgccgcc ggcttgctta
    10021 tcctttgcct gtcggcgccc gccgcggcgg cgccgcgcac cgtcgaggtt tacaatcccg
    10081 ccggtcaaca gcccgacggg gtctatgtgg acactatcct gaagaaaatc atggatgaaa
    10141 ccggcgtgaa gcgaccgctc ctggccggga aattcaccgg ggtaaagaag gagcggatcg
    10201 gcatccgcca tgatgagttc tccgaggtaa gcgagtggtt ttacctgcgg ggatggaccg
    10261 acggacttcc catcgtgccg ccgacggaac gacgggtaaa ggagatgctg aagggtacgg
    10321 acctgccggc cgattttctc gtggccaatc ttgaccccat gggcgggcag gcgacggtgg
    10381 aaaagatcgc cgtcaatgcc gtaatggcgg gctgccggcc cgaatatatg ccggtgctga
    10441 tcgccgcggt agaactggcg gccagaccgg agttcgactt gaagggtctg gcgacgacca
    10501 ccaaccccga tgcgccgctg ctgatcctga gcggccccat tgtggaacag ctcgatgtca
    10561 atgcgggaac caacaccctg ggacggggct ggcaggccaa tgcctccatc agtcgggctc
    10621 tccacctgat catccagaac atcggcggca gttggccggg cgtgacggat atgtccacca
    10681 tcggccagcc gggggatttc gtcatgatgc tggcggaaaa cagcgccgcc aacccctggg
    10741 gaccgctgca catggaactg gggcaccccc gggcggcaaa cgtcctggcc gtcatggccg
    10801 ctgaggggac ccacagcatt ctcgggatcg gtcagtccga cgaggggttc ctcaaactgg
    10861 tcgccgccca tctggcgggg ctggaaagac cctaccgcga gacgatgctg ctgatcatcg
    10921 cccaggatac ggcgaagatg ctggccgaca agggatggac cagggaaagg atgatcgcct
    10981 acatcaccga ccgggcgaag atccccttcc gcctgtacaa ggaacgctat ctcgattcgt
    11041 cggcgctgcg gaagggcatg ccggactggg tcttccgggt gcaagatccc gatgccatga
    11101 tccccagccc cctgatcggc aaactgttga ttctcgtcgc cggcggcacg ggcgagaaga
    11161 gcaccctcat tccgggctgg tatgatgcgg gcatcatgag caaggagata cgcctaccgg
    11221 cgaactggga cgagctgctg ccgaagaaaa acccctgacc cgccagcgag ggagggctta
    11281 gccccatccc caccccgacc ctccccttga aggggaggga gatttgcgac ttttgcgaga
    11341 tcgccaagga tgacgcccgg agaggctatt ttttccagac aaactgttcc tgctccacct
    11401 gcgccggatc cacgcccagg ctctccagct ctttcagggc ggtgtccatc atggggggcg
    11461 gaccgcagac atagcaggcg gcgttttcct tcacgccatg cttcttcatg atctcggcgc
    11521 tcagccggcc ttgttcatac ttcacccccg gaatgcgggc gtcgaaatag ctctgtacgt
    11581 catcttcccg gctcaggcaa tggacaacgt tgagcggcag ctcgccggtc atcgcggcaa
    11641 tttcctcccg gcagaagaca tcggcgagag tcttgttcga ccagaagagg ataacccggt
    11701 ttttggcccc gcagttgcgg aagtgcctca aaacgctgag aaaaggtgtg atgcccacgc
    11761 cgccggccag catgacgatc tccggccggt cgtcaatccc ctggcagaaa acaccgagag
    11821 gtcccatcac cttcaccggc gtccccggcg tcagggaggg gatggcggaa gtgaaaacgc
    11881 cttccttttt gatggtcagt cgcaggaggt tatcctccgg ggcggcggaa atggtgaaag
    11941 ggtgcggttc gctccagccc tctgcggccg gaatcctgat ggagacgaac tggccggctt
    12001 ttctggcggt gaacttctca tcctttccct ccagaaagag cgagtttaca tcactatttt
    12061 ccccggccac ccggaccact ttccagatgt ttttttcttc acttgcggta tttgctgaag
    12121 ccattgcgga acctcctcgt gatttaggca aagatattaa catatcttta tcacggagca
    12181 catcgttatt tactccggaa caccgaaaat ggcgggcaat gtcagcggcg ccggtgaacg
    12241 atccgcttgc cggccgacac caggatgtag gccagaccgg caatgacgat ggtcatcgcc
    12301 ccagcgggaa gatccggcct atagctgacc gccagaccgg ccgtggtgaa aaagggggat
    12361 catccagaga tcccggggcg agaccatcag gatgctgccg aaaagataac tcatcagatc
    12421 ttcgccatag ccgggggtcc gggatcagaa gtaatgtctt atcaaacatc ctgtctttca
    12481 ataatacgtg ctcacggata accgcaagga tggcgagact gccagcgcca ggcatcggcg
    12541 cacatctcct ctagccctcg cacggcccgc cagttcagat cctttttcgc caagcccgga
    12601 tcggcatagc agacggcgat atcgccggaa cgccggccca cgatttcata gggcaccggt
    12661 ctgcccgcgg ccttttcaaa ggcccggatc atctccagga cactgtagcc ccggcctgtc
    12721 ccgagattgt agataccaaa atcgccggcc tccgcaaggc gctccagggc cttcagatgt
    12781 ccttgggcga ggtccacgac atggatgtag tcccggaccc ccgttccatc aggggtgggg
    12841 taatcgttcc caaagacgag gagacggtcc agcttaccca cggccacctg gctgatgtag
    12901 ggcatgaggt tgttggggat gccggccgga tcttccccca tcaacccgct ctcatgtgcg
    12961 ccgacgggat tgaaatagcg cagcaggatc acatgccatt cgggatccga cgtcttcagg
    13021 tcctggagga tctgttctat catcatcttc gttcgaccgt aggggttcgt gggacccaga
    13081 ggaaaatcct cacggatggg caccgtggcc ggatcgccgt aaaccgtggc cgaagagctg
    13141 aacacgaggc gcttcaaacc atggtctgcc atgacttcgc agagggcgag ggtcgagccg
    13201 acattgtttt catagtaaaa aaggggctgg gttacggact cgcccaccgc cttgagacca
    13261 gcgaaatgta tcaccgcgtc gccgggatgg acttcgaaca ccgccctgag gaagcttcgg
    13321 tcccggcaat caccgtgaaa aaacgccggc cggcgcccgg tgatggtttc gatccgggac
    13381 aacacgctga ccttgctgtt ggaaagattg tccaggatga cgacgtcatg gcccgcccgc
    13441 aacagttcca cgcaggtgtg actgcctatg tagccggcgc cgccggtgac gagtatcttc
    13501 atatccatat cctcaatgat tctttcggga ctccgggggc aattgccccg tccgggaaag
    13561 agttcttatc atatttatcg tcccgaggct gcaatttttt agtccccccg gcgacgggaa
    13621 acttgacatc tcgggatgat gatatatcat gcatactcat aatccatgaa aaggacggat
    13681 cgaaaaatgg ggtacatcaa gatcaagttc aaaaacgagc ggggcaagtt cgagcaggag
    13741 attcaggatg tgctcgatga catgtttcat atctccggac cggcgttcac cctcggagaa
    13801 catacctggc ggccccatgc ggacatctac gaaacctcgg atgcaatcgt tgttatcctc
    13861 gatttggccg gtgtcgtccg ggaagacatc catctggagg tcagccgcaa aaccatcaag
    13921 atatccggca aacgtgacca gagttatttt tccggaacga cgcggtacca tctcgccgaa
    13981 atctcctatg gttatttcga acgccaactg gccctgcacg cccccgtgga ttccgaaacg
    14041 gtccaggcga cctatgccaa cggcctgctg gaaatccggc tgataaaaat tccgccggac
    14101 cgggaaaggc gcatccccgt tctgggcccg accggttcgg aatcgtgacc acaatataac
    14161 gacagaggac cacggacatg actgaagaaa aaaccgctca agatttgaag gttatccata
    14221 tacccgagac ccttcccgtc ctgccgcttt tcaacatgat cgtctttccc aagatgatgc
    14281 tgcccatgga ggttttcgga gaccagtccc tgcaactcgt cgatgaagcc atggcgaaag
    14341 accggattat cggtctcgtt ttatccagaa aaagcccgcc ggagacgacc tacgatccgg
    14401 aagattttca cgccgtcggg accagcgccg ttatcctgaa gatggccaaa gcggcggaga
    14461 agaaggccca gctcctcgtc caggggatcg agcggtttcg gatccgggaa ttcatccccg
    14521 gcaagcccta tctccaggcc cggattgaat cgatccagga acaggaaggc aaggatatag
    14581 aaatcgaggc cctcatggcg aatttggtga gcctcttcga gcggatcgtc aaactgactc
    14641 cttttctccc ccaggaattc ggcgccatgg ccaagaccat taccgatccc ggcgttctgg
    14701 cggacctgat cgcctccatc atcaacgtct cggcggagga aaagcagggc atcctggagg
    14761 ctacggccac caagaagcgc ctccgtgagg tcacccgcct cgtcaaccac caggtggaaa
    14821 tcctcgagtt gggcaataag atccagtccc aggtgaagga tgatatggac aagagccagc
    14881 gcgattatta cctgcgccag cagttgaccg ccataaagaa ggaactgggc gaagaggacg
    14941 aaaccaaggt ggaggtggag gaataccggg aaaagctggc cggcaaggac ctccccgagg
    15001 aggccaggaa agaggcggag agggagctgc agcgtctggg gcggatgcat ccatcctcgg
    15061 cggaatataa cgtcgtttcc acctatctgg actggatcct caccctgccc tggcgggagg
    15121 gcacggagga caacctggac atcaaaaagg cccgcaaggt cctcgacgat gatcactacg
    15181 gcctggaaaa gcccaagaag cgcatcatcg aatacctggc cgtccggaag ctgaaaccgg
    15241 acacgaaggg acccatcctc tgcttcgtgg gccccccggg gaccggcaaa acctccctgg
    15301 ggcagtccat cgcccgctcc ctgggccgaa agttcgtccg catcgccctg ggcggcgtcc
    15361 gggacgaggc ggagatccgg gggcaccgcc gcacttacat cggcgctctg cccggccgga
    15421 tcatccagag catccgccgg gcggaaacca ataatccggt gttcatgctc gacgaaatcg
    15481 acaaattggg cagcgatttt cgcggcgatc cctcctcggc cctcctggag gtcctggacc
    15541 cggcccagaa tttctccttc atggaccatt atctggatct tcccttcgat ctttcccacg
    15601 tgatgttcat caccaccgcc aacatcctcg acacgatccc gccggccttg cgggaccgcc
    15661 tggagatcat cgaactggtg agctacacgg tggaggaaaa gctccacatc gcccagcgct
    15721 atctcctccc ccggcagatc cgggagaacg gcctgcagcc aagccagatc accttcacca
    15781 aaggagcact ccgggtcatt atcgggagct acacccggga ggcgggggtg cggaacctgg
    15841 agcgggaaat cgccgccgtc tgccggggtg tcgccagcca ggtggccgtc ggcgacatcg
    15901 agcgggccaa gatccgccgg caggacatcc accgctatct gggtccggaa cggatcacca
    15961 cggaaacgac cgcccggacc tcgaaaccag gcgtcgtcat ggggctggcc tggacgccca
    16021 caggcggaga tctcctgttt atcgaggcga cggccatgaa ggggaaaaag ggacttaccc
    16081 tcaccggaca gttgggcgac gtgatgaagg aatcagcgtc ggcggctttg agcttcatcc
    16141 gggccaacgc cgaatcgctg ggaattgccc gggatttctt cgaggacgtg gacatccaca
    16201 tccacgtacc ctccggggcc atccccaaag acggcccttc ggcgggggta acgatcctca
    16261 ccgccctcac ctcgctgctc accaacaaac ggatcaagaa agacctggcc atgaccggcg
    16321 agatcaccct ccggggcctc gtgctccccg tcggcggcat caaggagaag gtcctggccg
    16381 cccaccgcgc cggggtgaag accatcatcc ttcccaaatg gaacaagaag gatctgggcg
    16441 acatcccggc caagattcgc aaggacatcg ccttccacct cgtggacgac atgctggaag
    16501 tcctgaggat cgccttggag aaataggcgc ttcccaactt acaggagccg gtttgacgac
    16561 ccggagagaa gatccgaagc agccctcggt gagcatttgc acctgatgat tgccggaagt
    16621 tacagcgccg acgaaccgta aggggtgttt tcccccttga cagggaggga aaatcttgct
    16681 accaaccgtt tagttgaacc aaccggatgg tttatgatga cggacaaaca atccctgacc
    16741 accgcgcgca tccttgacgc cgccacggat atcttcgccg aggcgggttt caacggcgcc
    16801 cggatggatg aaatcgcccg ccgggccggt gtgaacaagg ccatgatcta ctaccacatc
    16861 ggcgccaagg aggccctgta tgccgaggtg ctgcacggcg tcatcggtca tgtcgccgat
    16921 tcgatttcgg cgtgcatcga aaccggcgct actcctgaag caaaactgcg ggcctacatc
    16981 cggggttttg ttgacgccgt ggagggcaat ccccgcatgg gacccattct cctccgggaa
    17041 atcgcctcgg ggggccagaa tctgccggac ctggtgatcc aggacttcgc ccggatcatc
    17101 gccctcatca ccgccatcct cgatgaaggc cgggaacggg gcgtttttta tccggtgacg
    17161 cccttcctca ttcacatgac ggtagtgggc tccctgatcc tctacaagac cagcggcccg
    17221 atccggagcc gcagggaaaa cattcccgcg gcggtcaagg ccctggatca gcaaatcacc
    17281 ccccgggtaa cggtggaggt cgaaaacctg atcctccggg gaatcaaaaa gcctccccgg
    17341 ccagctgccg aggtaacgaa cgatgatgat gacccccgtt gaaaaatccg gccgccggtc
    17401 atggccgccg gccgtcagca accagcccgt aacgcccccg ccggggacgg cgatcccggc
    17461 cagaccaggc gtcccccgtt tcggtctcct ggctgtcgtc gcgctgcttc tcgccttcct
    17521 tcccttctcc ccggtcctgg gacaatcccc ggcggtccgg ccggaaatga cagcggccac
    17581 tccctccacc gctccatgcc ggttcacctt ggcggagagc gtggccatgg ccctgaaaca
    17641 gagcatggtc gtccaggcgg ccaaggaagg ggtgaagggg gcggaggccc agaaaaagga
    17701 ggccttcacg ggctttctgc ccaaactcag cacctcttac agctacaccc gcctcaacga
    17761 tgcgccctgg accaacatgc cgcccatgac catttcctcc cccatccttc ccgcccccct
    17821 gacgacgccg ggcggtccga tcaccgtggg cacccaggac aattacagtt gggccctgga
    17881 gttccgccaa cccctcttcg ccggcggctc catcctggcc aactaccagg ccaaccggaa
    17941 gggcgaggac atcgcccggg tggatgaaac ggccgccgcc cagaatattg tcgaggaggt
    18001 gaaaagcgcc tatttccaga tcctcaaggc acagaagatc ctcggcgttg cccgccaggc
    18061 ggtggagcag cttaagtccc accgggacga ggcgcgtcac ttctttgacg tcggactgat
    18121 tccgaaaaac gatctcctcc aatcggaggt tcagctcgcc aacggggagc ataatctcgt
    18181 ccgggcggaa aacagcctgg agatcgtcaa gtcccgcttc aacaccctcc tgcggcggcc
    18241 gatcgacgcc ccagtggaag tggaggacat cctggcttac cgtcccttcc ctaaagccgt
    18301 cgaggaatgt ctccaaaccg cctacagcca gcggccggaa atcaaggccc acgccctgaa
    18361 ggtagaacag gcccgggagg tggtcaaggc cgcccggggc aacttctttc ccacggtgag
    18421 cgccgtgggc cattacgaac ggttcgggga tacgggcagt ctccagggca gcacctacaa
    18481 gagcatggaa aactggtacc tcatggcccg ggcggactgg accttctggg aatggggaaa
    18541 gacgaagaac caggtggacg tccaccgcgc ccgggagaat caggccggca gcatgctgat
    18601 cctcgaacgg gacaaggtga ccctggaagt gaaggcagcc tacatcctgg tccgggaatc
    18661 ggaaaagcag gtctttgtcg cccagaagtc catcgatcag gccgaggaga acttccggat
    18721 caacaacgag cgctaccggg aacaggtcgc cacatccacg gacgtcctgg acgcccagac
    18781 gctcctgacg aaggccaaat cggattactt cacggccctg ggggattact tcatcaacca
    18841 ggcccggctg gagagggcca tgggggtaag tcaatgaaga aacgcgtcgt cctggccgtc
    18901 tttgtcgccc tgctcgtcgc cgtgggcgga tacgtctatt acggccagtg gaaaaacaag
    18961 catcgggaaa cctattattc cggcgtcatc gaggccaagg acgccaaact cgccttccag
    19021 gccggcggcc gggtcatcgc cgtccacgcc cgggaaggcc aggcggtgac aaaggaagag
    19081 gtcttggtgg aattggatgc ggcggaattc cagagccgct gcgaccaggc ccgggccgcc
    19141 ctcgcccggg ccgttaaagg tcaggagcag gcggaaacct cacttcaggt gtatcagaag
    19201 gtcctgccgg cggacgtgat ccgtgccgag gccgccgtca cgagcgcccg acgggtcatg
    19261 gaggaggcgc ggcggaacaa ggatcgttac gacaaactgt accagcgaaa ggtcgtcgcc
    19321 gaaaaggaat gggatgccgt gcggttgcat tacgatacct ccgtttcccg cctggccgag
    19381 gggaaggccg ctctcagcca agcggtaagc aacctgaaga agatcgacat cgcccggcgg
    19441 gaaatcgaag cggcgaccgc ccaggtccgg ctggcacggg catccatgga acaggcggag
    19501 atccaggcgg cctacacgaa gctccgggcc ccctttgccg gcgtgatcac cagccgcaac
    19561 gtcgaacccg gcgaggtggt ctctcccagc cgggaggtct tcaacctggc ggatctctcc
    19621 cgggtcgatc ttaagatctt cgtggaggaa acggccatcg gcaaggtccg gccgggacag
    19681 gcggtggagg tgaagatcga caccttcccg aaccacgtct tcccaggcgt cgtttccttc
    19741 gtatccccgg agggcgaatt cacccccaag atcatccaga cccagaagga gcgggtgaag
    19801 ctggtttatc tcgtcaaggt ctccataccc aatcccgact tggccctcaa gaccggcatg
    19861 ccggccgacg cctggctgaa atagatggca ctggccagcg aatcgttccg gactccggta
    19921 ccgcctctgc atttccgacc cgatcgcggc gggacggagt caatatctct tcccccggcc
    19981 gagaggacag gaacatgacc gcacacccgg actcctttgt gagcatcgag cacgtctcca
    20041 tgcgcttcgg cgccgtcgag gccgttcacg acgtcactct caaggtgacg gcgggaacga
    20101 ttttcggcct ggtcggctcc gacggcgccg gcaaatccac tctcctgcgc atgatcgcca
    20161 ccatgatcaa accggcggcg ggacggatca ccgtggacgg ccacgacgtc gccgccgaaa
    20221 agaaccgggt gaaggacctg atcggctaca tgccccagcg cttcggcctc tatgccgacc
    20281 tgacggtgga cgaaaacctc cggttcttca tggacatctt cgggattccc cgcccggaac
    20341 ggaagcggcg gcgggaaaaa tacctcggtt tttccaatct gttgcccttc gccgaccgcc
    20401 ccgccggcgc cctttccggg ggcatgaagc agaaactggg attggcctgc gtcctgatcc
    20461 acgaaccccg cctcctaatc ctcgacgagc cgaccaacgg cgtggatccg gtttcccgcc
    20521 aggagttctg ggaaatcctc ggggagatgc gccgggaggg catgactatc ctcgtctcca
    20581 cggcctacct cgacgagggg gaaaaatgcg gcggcctggc gctgatgcat aaggcccgaa
    20641 tcatcgacgt ggcgacgccg gcggctatcc ggaccgcctt cccctccctg gaggccgcca
    20701 tgatcagtcg ccttcaggag gcggacgccg aaatcgggaa agatgccttt tttgcccatc
    20761 ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncaatcagc gaacggtatc
    20881 gccattcgag tcaaagacct ggagaaacgc ttcggcaagt tcacggcggt gaacagggtt
    20941 accttcagcg tccggaaggg agagatcttc ggttttctcg gcgccaacgg ctcgggtaaa
    21001 tcgactacca tccggatgct ctgcggtatc cttaccccga ccgccgggga agggatggtg
    21061 gcaggctacg acatcttccg ggaacccgag gagatcaagg gcgccatcgg gtacatgtcg
    21121 cagaaattct ccctttacga ggatctcagc cccggggaaa acatccgttt ttacctgggt
    21181 gtctataacg tccccccgta ccgctgggag gagcggaccg cctggattct cgagacgact
    21241 caactgggcg acgtccgcaa ccgcctcacc cgggaactcc cccagggatg gcgacagcgc
    21301 ctcgccctgg gatgcgccct cctgcacgag ccggagatcc tcttcctcga cgaacccacc
    21361 tccggcgtcg atcccgtgac acgacagcgc ttctgggatt tgatccgccg gctggccgcc
    21421 gggggcatca ccatcttcgt caccacccac tacatggatg aagcccggca ctgcgagcga
    21481 atcgtcatgc tcaaccaggg ggccatcgtc gccgcgggaa gcccggagga gatcatcgcc
    21541 cggtcctgcc cggaccggga ggatgcggac ctgaacgacg cgtttattac cttgatgaag
    21601 aactgagaat tatacctgac acgacgggaa acccatccac accccaactt tcttcttgaa
    21661 ggggagggag aaaagcatcc ggtaagatga acctacggat aattgccgcc ataacccgca
    21721 aggaatttta tcatctcatc cgggactacc ggagcctgta cctcgccttc gccatccctc
    21781 ttatccttat cctcctgttc ggctacgccc tgagcctcga cgtggataac gtccggacgg
    21841 tcgtagtcga tcacgacaac accccccgga gccgggacct gatccgccgc ctggatgcct
    21901 caccctattt ccacgtcgcc gctcggctgg gaaactcgga ggatgcggcg gcctggctgg
    21961 atcacggccg gacgatcctg gcggtggtca tccccccgga ctggacccgg aatatccagg
    22021 cggaccggga ggagccgctc cagatcctcc tggacggcag cgatcccaac ttcgccaaca
    22081 tctccgccgg ctacatcacc gcgtttctgg agcgggagaa caccgcggcc cttcataatt
    22141 atctcaatcg ccaggggcag gagcaactgc ggcccccttt ggaaggcagg atccgcatct
    22201 ggttcaacga ggatctggaa agcaggaatt tcatcgttcc cggcatcatc gccgtgatca
    22261 tcatgatcgt gggcgccatg ctgacctccc tggtcattgc ccgggagtac gaaaacggga
    22321 cgatggagac catcaagtcc ctccccgtga gcgccgggga tttcattgtc ggcaaggcca
    22381 tcccctattt cttcatcacc ctggccgacg tccttatcgc catccttatg ggtcaggtcc
    22441 ttttcggcat cgtcatgaag gctgatttct ggctcatgat cagcgcctgc gccctttatc
    22501 tggccgtggc catcaccctg gggctcttca tctccgccgc ggtgaagtca caactggtgg
    22561 ccaaccagat tgcccccata gtcaccttcc tcccctccat gctcctctcc gacttcgtct
    22621 ttcccatcat gaacatgccc gcggccattc aggtcatcac ctacatcgtc ccggcccggt
    22681 attttatcga catcctcaaa ggggtttaca tgaagaacgc cgacttcacc ctcctgtggc
    22741 cgaactttgt cgtgcttttc atcatggcgg ctggcttggg ggccttgaat ctaatcgtct
    22801 tgaaacggga gggcatgtaa catgtgccgc ctgcggatcc gggaactggt gcggaaggaa
    22861 ttcattcaga tctttcggga caagcgaaac cgccccatcc tcatcgtggc gcccctgatc
    22921 cagatcctga tctttggcta tgtggtgaat ttcgatatca acattatcca tgtcgccctc
    22981 ctcgatcaat cccgaacctt ggagagccgg aagatcgtcg atgccttcag cgccaaccac
    23041 ctgttccaga tcgacaacat tgttcataac tcacaggaaa tggaagagat cctcatccgg
    23101 gggaaggcgg acatcggctt gaagatcggc cccgatctcg cccggaagat ccgccggggg
    23161 gaaacggcgg aggtccagat ccttgccgac ggcacggtga gcaatatggc ggcgatccgg
    23221 gtttcctacg tcatgaccgt catggaacgc ctgaaccgtc agttcctcca ggaccgcttt
    23281 ccggcacagt tgagttacgg ccgtatcgac ggccgcctcc gcacctggta caatcccaac
    23341 ctctacagcc gtcacttctt cgttcccggg atcgtcgcct tcatcgtcat gctcatctcg
    23401 cttttgttca cctccatggc agtcatcaag gaaaaggagg ggggaacgat ggaacagctc
    23461 atcgtgacgc cgatgaaacc gtacgagctg atcatgggca agaccatccc ctacatcgtc
    23521 atctccctga tccaaatggc cggcgtgctc ctcttcgccg tctatctgtt cgatctgccc
    23581 attcggggca gtctgtccct gtttttcctc gccatctgcc tctttctcat gagcaccctg
    23641 gggatcggcc tgttcatctc caccatctcc gccacccaac aacaggccat gatgtccacc
    23701 ttcttcttcc tccagccgtc ctttctcctc tcgggcttcg tcttccccat cgccaacatg
    23761 cccgtggtcg tccagtggct gacctacctg aatcccttcc gctacatcct cgtgatcatc
    23821 cggggcattt tcctcaaggg cgtggggatg gacgtcctgt ggccccagta tctgtccctg
    23881 acgatcctgg gcctggccgt cttcactgcc gccgtcctgc gcttccacaa acgcctggat
    23941 tgaatcttcg aaaacggtat atttccgtca aaattctccc tgacaggcaa acgccccgac
    24001 tctgcttgtc aatcggcaga tgcatttttc atgattttgg gttatattca tcagcaatta
    24061 cggaagataa ctctttcgat gtcattccga acgagctgat tcaaacaatt atataatgat
    24121 atcaatctta aaatttaaag gtccgtacta aaacattgta tcggaggaaa gatgaaaggc
    24181 acgatctacc gtcagtgtgt tgggttgggc aatgaatccg tttcgaccct gaaaaggctt
    24241 ctccatgaaa tgccgcatcc cttccggaaa ggagataata ttgggatcaa actccattgg
    24301 ggggaaaagg ggaatcacac ctttctgccg ccggtattcg cgcgggagat cgtagtctgg
    24361 cttcgggaac tgggagcgac gccctttgtt tttgatacct cggttctcta ttcaggcagc
    24421 cgtcgcaacg gaagcgacag cctgaaaacc gcggcagcac acggttacag cgaagcatat
    24481 ctcgactgtc cggtggtcgt cgccgatgga ctcgatggac gggccgtcat cgatattccc
    24541 gcgggataca ggcatttcga aacggtacag gtggcggaca tattcaaaag ggcgtccggt
    24601 tttgtcattc tttcccactt caaggggcac atggtttcgg gattcggcgg cgccataaag
    24661 aatatctcca tgggctgggc gtcgcgggcg caaaagcaac gcatgcactc cgatgcgcgt
    24721 ccgcggctgg aaaagaacag ttgctcgcaa tgcggtctat gtgtagacat ctgtccctcg
    24781 ggtgcggcgc aaattgccgc ggacgggtat cctgattaca gtcaggaatt gtgtatcgga
    24841 tgtgctcagt gtatcgggct ctgtccggaa ctggccctgg aaatatgctg gaacaccgac
    24901 atcaatgtgt ttcaggaaaa acttgttgaa acggcggctg cgatctggaa gcaaatccag
    24961 ggccgggccg tgcttgtcaa tgcaatgctc aacattgcct cggaatgcga ctgcctgccg
    25021 ggacaacatc ccattatcgc tcccgatgcc ggatttgtcg gcgggtacaa tcccgtgtcc
    25081 gtcgatgtgg agtccataaa caggatcggg aaagagcctt tcataaaggc ccacccgcat
    25141 gtggattgga aacaccagtt ctcatacggc agagaaattg gttttgcgtg aacagccgcc
    25201 aattgcgtga ttttctaaaa tcttctattg gaacttgagg tcgttttgag gcgccacgca
    25261 tctttactga ccggcggtca tttttttggg ggtaaaattt ttttcagatc gttaatttct
    25321 ttattatatc ataagcttgc tgaccgtcga ttctcgacgc tcctcgccgg ggataccgtt
    25381 gcgccgtccc ggccagttaa caaatatttg ctttataata ttataatata gttttataac
    25441 cagattataa gtgccttgcc gctgcggggc atgaatccca tgaacccatc aagacggtgg
    25501 gcaaaagagg tccttacgat ctcgaaaatg attgacgagc ggttcgggag gctttattcc
    25561 ggataccctt tccggacggt tcctcgcgtt tacgcccttg ccttacgtcg gccattgtgg
    25621 caatgaagta atcagcgtcc ggaagatcga aaaacgggag aattcgcggt catcgccaag
    25681 aggcgctaaa acggcgccgc gccgggaacg gcagcggctg catgttcgca gcggctgcat
    25741 gttcatggaa agcgtcaaat tttcctggac gaggaaagca ttatcgggca agatagatga
    25801 catcgtaaaa agcgcaaaaa atgtcccatt tgggacggca tcgtaaaaa
//
LOCUS       DAOSMF010000019        24664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_2563_length_24664_cov_25.889146, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000019 DAOSMF010000000
VERSION     DAOSMF010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 24664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24664
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2563_length_24664_cov_25.889146"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1070)
                     /locus_tag="PLT63_03905"
     CDS             complement(<1..1070)
                     /locus_tag="PLT63_03905"
                     /inference="COORDINATES: protein motif:HMM:NF013811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOD97901.1"
                     /translation="MIFPLQTRRFSIQVTGGRHPWPVPANKPKNSGPPDIPTDRNGRP
                     RHPHGEIVIMEPTGNYPLSEAVRESYRAGEEDETLQPLVAGGKDGAPLGRIGKRDAVI
                     FYNIRGEREIELTRSLTEKGFAEFPTAPDLGLAYATMIEYRRDLPVEVAFPPEETLEN
                     TLSALIARAGLKQVKITEAEKAVHVSYFLNGKRQEPFPGEDRIVVPTRKDVALFDAAP
                     EMSIGEIGAAIADAIHDQRYSFIFANFPNVDVVGHIENREAIIRAIEAVDLQAGAAIA
                     AAAEAGMTVVVSADHGTVEKWLYPDGAVDTGHTDSPVPFALISPDGRITLRRQGELAD
                     AAPTVLDLLGLPPSPLMTGRSL"
     gene            1181..1570
                     /locus_tag="PLT63_03910"
     CDS             1181..1570
                     /locus_tag="PLT63_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97902.1"
                     /translation="MKGKTIDDILREEFLKERAVVLGRTGESVARAIARLQHLAQIIE
                     NRHQELRRLDREPDGATECHELRTAKESAVNDINSKIAQYNRLWDHAQLRYYYLIVTR
                     EAMGLRSHHRVEEIYRIPPKKKQLSGG"
     gene            1570..2223
                     /locus_tag="PLT63_03915"
     CDS             1570..2223
                     /locus_tag="PLT63_03915"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOD97903.1"
                     /translation="MDRFQKLRIKMVETQIRARGVTDERVLAAMAKIPRHLFIDEALI
                     EQAYNDNPLPIGKSQTISQPYIVALMTAALKLTGKEKVLEIGTGSGYQTAILAELAEQ
                     VFSIERIAQLAAGARKRLDALNCFNVAMRVGDGTYGWREESPFDGIMVTAGAPKVPTI
                     LLEQLAVGGRLVIPTGGRISQELLRVTRMSADLNEVKTEVLCGCRFVDLIGEHGWNN"
     gene            2226..>3049
                     /locus_tag="PLT63_03920"
     CDS             2226..>3049
                     /locus_tag="PLT63_03920"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOD97904.1"
                     /translation="MPPDPAVLKKLAMRYLAVLMALLSLLVFSCAGQKNVVRPGKGVY
                     HLVKKGETLSQIALAYGVSQRKLVEANNIRHPDHLEAGSVVFIPDAVRVLDVGKVLQA
                     KATVDQPEKGPAKKSGEKKPPSVAGKKERKPAELPREGTPKPGVDHRKGGAGKPAKEK
                     NRDAVPTAAPPPPPLPGTAASASPGKVSFAWPVRGRVASRFGRQPNGMVFNHIRIATR
                     DHAQIVAAASGTVIFSAPLRDFGETVIIKHDDDFATVYTHLGSRSVRADSRIKKGE"
     assembly_gap    3050..3149
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3150..4142
                     /locus_tag="PLT63_03925"
     CDS             <3150..4142
                     /locus_tag="PLT63_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOD97905.1"
                     /translation="DDIRNARMIADEIRALSWKLGGGGRVTDAVVRRVYSGGNGYNDA
                     FRSVCAWLYWGGESVFLQDANSLILKTDELVEILSFVKEQFPGVNRITSYCRSKTAAR
                     KTVAELEKIREAGLARIHMGMESGYDPLLEFIRKGATVADHVEGGKRIVAAGISLCEY
                     IIPGLGGESWSREHAVATAAAVNAINPDYVRLRSLQVVKGTGLYERMQNGEFTPQGDE
                     ETVREIRLFIENLTGVRTTIVSDHILNLLEEVEGKLPDDRDRILAVIDTYLSLPEQDR
                     MIFRLGRRQGIYRRLADLNDPVMYGRLKAVTDEYVPDGAERFDADLKELMHHYI"
     gene            4211..4696
                     /locus_tag="PLT63_03930"
     CDS             4211..4696
                     /locus_tag="PLT63_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HOD97906.1"
                     /translation="MFKIGDMAVYPAQGVGVIENIENREIMGTKQTFYILKIMGNGMK
                     IMIPTKSAKSVGLREVISEKEISKVYDILKNKEVTIDKQTWNKRYREYLEKIKTGSVY
                     EIARVLRDLLILKNDKNLSFGERKMMDTAKNLLVKEISVATKSDETKIEEDLKTIFTI
                     Q"
     gene            4871..5899
                     /locus_tag="PLT63_03935"
     CDS             4871..5899
                     /locus_tag="PLT63_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HOD97907.1"
                     /translation="MIVRIILIAACSISGFFIAYLTYPPSQWWTWPIGFVIGLVIAFF
                     VIKVEQDIRKFSLRIILGGVVGMIVGLIIAFLLAYGLNFVSNIKHNQEVVPWIYALLT
                     GIMGYLGLVLGSKKTEELNIFGFGQTKEPDEARVLDTSVIIDGRIADVCETGFVEGTL
                     MVPRFVLDELQYIADSSDTLKRSRGRRGLDILNRMQKMAGITVEVGDQDFPKVKGVDA
                     KLVALARKINAKIITNDFNLNKVAELQGIKILNVNELANALKPVVLPGETMTVKIIKE
                     GKEPGQGVAYLDDGTMIIVDNAQKFQGAVVDVAVTSVLQTTAGRMIFSEMKALAGDKK
                     PFSLSVKS"
     assembly_gap    6016..6115
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6171..6899
                     /gene="ispD"
                     /locus_tag="PLT63_03940"
     CDS             6171..6899
                     /gene="ispD"
                     /locus_tag="PLT63_03940"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOD97908.1"
                     /translation="MMISEFGHGGLGKVQATAIIPAGGSGRRMGGTEAKQYLLLQGIP
                     LLVHTLRVFQEAAVIGEIILAVPAADVDRIRREILDPFRLTKVRHVVAGGRQRQDSVH
                     NGIAALADDCELVVIHDAARPFVTPDLIARAVEGAAQYGAVAAGIPARDTIKYCDAAG
                     RVARTLPRERIWIVQTPQAFRRETIVAAHRQARRDNFSGTDDAALVERLGVAVRMIVG
                     ESENIKITTPDDLVWAEHILSAQR"
     gene            7035..7550
                     /gene="ispF"
                     /locus_tag="PLT63_03945"
     CDS             7035..7550
                     /gene="ispF"
                     /locus_tag="PLT63_03945"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOD97909.1"
                     /translation="MTGSGAGGIKTARVGIGYDSHRWCDGRPLVLGGVEIPHSRGLSG
                     HSDADALIHAICDAVLGAVGAGDIGAHFPDTDPAYRGISSLVLLARVREIAAARGGRV
                     VNMDATVIMERPKLRAYIGGMIANIADALAMDAAAVSVKAKTNEGMGFVGREEGVAVM
                     AVALVETAGAL"
     gene            7609..9207
                     /gene="gltX"
                     /locus_tag="PLT63_03950"
     CDS             7609..9207
                     /gene="gltX"
                     /locus_tag="PLT63_03950"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOD97910.1"
                     /translation="MDEKDKFTRISRGCRFMKDLPPRVRFAPSPTGDLHVGNARTALF
                     NWLFARRYGGVLVLRIEDTDQVRTSRVFEEHLLADLAWLGIDWDEGPGRGGPCGPYHQ
                     SERLPLYRDGLERLVAAGMVYPCFCTDEELEAERLSLLSRKLAPRYSGKCRRLDDDQR
                     RRLAESGRPAAWRFHVPPGSIAFRDLIRGEMRFAGEALGDFIIVRSNGIPAYNFAVVV
                     DDHLMGITHVIRGEDHLSNTALQLLLYRALGFEPPRFAHHALILGRDRAKLGKRHGAT
                     AVREFREQGVLPEALVNYLALLGSSFEAGREFCRVEETIASFSLDRAGKSGAVFDEDK
                     LWWLNSLYIRQEAPEILARRLRPFVAAAGYGEIGTSRLHGIIAAVRDNLHDLTEIGDY
                     LRIFDDDAYDLSPEARAIIADPEAREVIASLLDLLDGPVADVQGVQGDGGPEAQSRNA
                     AASATGAGEDAGIAGANDEVTPSLYRTVIELLAERTGRRGKKLFLPLRAALTGRTRGP
                     ELDRCFSVLGRASLRRRLERALAV"
     gene            9354..10712
                     /locus_tag="PLT63_03955"
     CDS             9354..10712
                     /locus_tag="PLT63_03955"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HOD97911.1"
                     /translation="MKLRIIEESVLFVSILKWIVLAAGIGFVVGVSTTVFLKILAAAI
                     AFAQDFPRYYLALPLGLFASVLIIKYMAREAQGYGTEKVIEAVHKNGGAMRAVVIPAK
                     LCATVVTIAAGGSAGQVGPCAQIGGGLAAAFANAFRLDANDRKKLVICGISAGFAAVL
                     GAPVAGAVFGLEVLFVGSLLYEVLLPSFIAGIISYHTAAVLGIDYFHYPVHLQPVFTE
                     VFFLEIIGAGIFFGLCAILAIESLRLTRSLFDKIPVWPPLKGIIGGIFLIVLAFLTSF
                     RYLGLGMEIIADSLQGVKVAASAFVMKTLFTAVTFASGGSGGIIAPLMVIGAAAGAFF
                     AAMTGLDAATFAAVGLVSVLAGAANTPIAMSILAIELFGKGIGAYAAIACVISFLITG
                     HRSIFPTQVMAMKKSSSVEVELGQELDKVQARYKFREKSVIGTGIKMTKRIRKPGNGR
                     KD"
     gene            complement(10758..11132)
                     /locus_tag="PLT63_03960"
     CDS             complement(10758..11132)
                     /locus_tag="PLT63_03960"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HOD97912.1"
                     /translation="MPYQEATPSLRCIDDVTSRYYNRLVHIDNVEDDWSSCEEMHRPD
                     QQYRLGVIVDHNAVPPIPGRGSCIFIHIWQGPDRGTAGCTAMSPENMEMILRWLDPAA
                     FPVLIQLPETTYRRLRSTWKLP"
     gene            complement(11830..13092)
                     /locus_tag="PLT63_03965"
     CDS             complement(11830..13092)
                     /locus_tag="PLT63_03965"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOD97913.1"
                     /translation="MKYIFPLFRIYFTKKMLLMLVLGFASGMPLALTSSTLAAWMVTE
                     GVDIKTIGIFSLVGLPYAFKFLWSPLLDRFLPPFLGRRRGWMLITQGALIATIATMGF
                     FSPASQPLIVAFWAVALAFFSASQDIVLDAYRTEYLAPEERGPGAGIWIMGYRLAILV
                     SGALALIMSDHISWRSVYVIMGLVMLVGVAATFLCPDPEAGRPLRHGAAPPQSLQEAV
                     VQPFVEFFKRPGAVEILLFVIIYKLGDVAAAQMTTPYILQHIGFSRTELGTIFKGFGM
                     AATILGGIVGGALMTKWDLRRSLLVFGILQGVSTISFVLLEFTGRQLWALSTVIGIEN
                     ICGGMGTAAYTALLMGLCNTRFTATQYALLSSLMAVSRYVTGAPTGFLVVAVGWVWFF
                     VICTALAAPGLLLLIRYRKWAGGLETGP"
     gene            complement(13105..14115)
                     /locus_tag="PLT63_03970"
     CDS             complement(13105..14115)
                     /locus_tag="PLT63_03970"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HOD97914.1"
                     /translation="MPPLIGLNMSIESTAASGVERLFVPPDYVAAVAGAGGVPLCIPP
                     GEDLATIRKIIPRLRGFLFIGGADYRPEHYGGHPQPAEELVHPRRDRFDVALAKTILE
                     ETDLPVLGICGGCQLLSIIRGGALIQDIGTEWRPPGGGPSLPHSAKDRERGVGDEAPA
                     DFPDLITATTPAEETKEAETHRTGALAGEEKGMANGPTADKADKAATGESSNRPAASP
                     GRVHPEGSKDCYLHSVRFAPLSLATCTTGTPPGEALAANSFHHQAVHPERVGKDLVAS
                     AWTADGIIEAIESAPGSAWAEIGRFVLGVQWHPERMQEEAPHRRLFLVLVEAASRYRR
                     RK"
     gene            complement(14119..15693)
                     /locus_tag="PLT63_03975"
     CDS             complement(14119..15693)
                     /locus_tag="PLT63_03975"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HOD97915.1"
                     /translation="MKVILAGYNIDYETIMDIEDRCEDLEELTPETIAAAYARISRSP
                     RPVHELRAAARGEVDKARQSNRNIVFEMGHSSIAEHAVFNLDVLGVSRLLVEDIEKFR
                     LCSYTEKSQRYVLFKDDFVIPEEIKNAGLAGPFAEVVGRQNSFYRECYEQLRPHVFAR
                     HPELARDPANRSLLEGWAKEDARYAIALATETQLGMTLNARNLELMLRRLAAHPLKEA
                     RDLSAALYKATKSIAPSLVRYTEATDYDLLTRRELREAAAALFKEDSPDERDRRMTGD
                     GGGPEVEQVVQTPPGQRGDAIAPVTTLPEASRDDVFLAYVTPEADLRLLAALLHSSSR
                     RPLSSCLSRARRLDAGQRMNLVKTALRHLRSHDTVLREFEYVDLLFELTVSAGCFAQL
                     KRHRMATITAQGYDPGLGVTIPPSIAAIGMDESFRKLMDFTGKRYEQLRETEAAEAAS
                     YILTNAHRRRVALKVNARELYHIARLRADRHAQWDIRNLTASMLAAAEKTMPLTLLMA
                     TGKDGFADLYNRRFGN"
     gene            complement(15701..17512)
                     /locus_tag="PLT63_03980"
     CDS             complement(15701..17512)
                     /locus_tag="PLT63_03980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOD97916.1"
                     /translation="MPQHREPPASPAAPSSERRSNRIPSEAGNDIFTGEDGDLTDFYR
                     TFLDSISDMAYLKDESFRHIIANAPLLRFFGKRESEVIGRTDFELMPEGAARKCRMSD
                     TEALETDTIHTDEEPVGDRIYETRKFRVRIGKDKYGIGGYIRDVTDKRQVASALKASE
                     ETFRAIAETTPSAIFIIQDTKVRYVNLAFERITGYNLAELKWIDYWELAHGDYRELIK
                     VRGLARQRGEAVPSRYELKIVTKNGEQRWIDLAATNLEYEGRPAILGTAIDITDRVLA
                     EEAIRKSEAQYRLLINNANEAIVVAQSGVIKFANPKTMEMTEYSAEELLSRPFTDCIH
                     PEDREMVSERFQARVAGGSAYPEIYPFRCVTKTGKIIWVEINAVSFTWNGRGATLNFL
                     TDITARREAEDLIGQTIARMEKSIEAIIQVISATVEARDPYTAGHQKRVAELARAIAL
                     EMGLSPEQADGIRVAGAIHDLGKLSIPAEILSKPTRLTDIEYDLIKFHAQIGHDILQG
                     IDFPWPIARMIMEHHERMDGSGYPRHLKGQDLLLASRIISVADVVESMASHRPYRPSL
                     GIHKAIEEISSQQGVRYDADVVAACLRLLKDKNFKFR"
     gene            17973..19301
                     /gene="der"
                     /locus_tag="PLT63_03985"
     CDS             17973..19301
                     /gene="der"
                     /locus_tag="PLT63_03985"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOD97917.1"
                     /translation="MRPVIAVIGRPNVGKSTLFNRLAGRGKAIVIDEPGATRDRNYAD
                     GDWQGRPFVLIDTGGFEPAATDGILAQMREQTTLAMEEADIIVFLMDGRDGLTPADRE
                     IAAMLREIDKPVFHVVNKIDGPKHEQLAYDFYGLGVEEIFTLSAQHGQGVFELMDAMV
                     HLLPKEVEASREEERLRIAVIGKPNVGKSSLINRLLGYERTIANPMPGTTRDAIDTPF
                     ERQGRKYLLVDTAGIRRKSRVSLTLEKYSIVQALKTIDRCDIALILIDAAEGITEQDV
                     KIAGLAHERGTACILAVNKWDLVVKDNETIGVYVKDIKDKAKFLDFAPIIFISALSGQ
                     RVTKIFDLIEKVYEQYTRRISTGDLNRAVREFTDRNPPSRSGGSPNAFAYVTQVAVKP
                     PTFIFFVRDPRAVHFSYERYLANQLREAFGFDSVPLRIFFKKKSKSQLSS"
     gene            19323..20531
                     /locus_tag="PLT63_03990"
     CDS             19323..20531
                     /locus_tag="PLT63_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HOD97918.1"
                     /translation="MDGLLTKTTGKGGYMVVKSSTRIFVPWLIGVGLAFMLSGFAAAA
                     DTIRLGVAGAHSGDLASYGLPTKRAAELVVKEYNAKGGVLGKKVEILAEDDVCKPEVA
                     TNTATKLVSGGVHVVLGHICSGATKTALGIYKDAGIVTMSSSATNPDLTQSGRYPNFF
                     RTIASDDAQARLDAGFAMDVLKVKKIAVLHDKGDYGKGLAEFARSFIEASRKAKVVLF
                     EGITPGAVDYSAVVNKIKQSGAEAVIYGGYHPEASSIVIQMRKKRMNTYFISDDGVKD
                     DTFIKVAGKAAEGVYASGPKDTTKNPLAIAAIAAHKKTYNADPGAFFLNGYAAAQALL
                     AAIQKAKSTKYAAVAKALRTYPVDTPLGKIKFDKNGDAIGVGFSMYQVKNGVYVEVPV
                     AAAPAPKKKR"
     gene            20532..21434
                     /locus_tag="PLT63_03995"
     CDS             20532..21434
                     /locus_tag="PLT63_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418203.1"
                     /note="LivHMGF is the membrane component of the LIV-I/LS
                     branched-chain amino acid transporter; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease LivH"
                     /protein_id="HOD97919.1"
                     /translation="MDYFLELFFGGLTRGSIYALIALGYTMVYGIIELINFAHGEIYM
                     IGAFTALIVSSVLAFAGFSGLSVLFLAAAVAIIYSAAYGFTVEKIAYKPLRQASRLAP
                     LISAIGMSIFLQNYVMLAQTSDFLPFPSLLPELPFLEELELSIGSTELIILMTTAVSM
                     VLLTLLIKFTKMGKAMRATAQDRKMAMLVGVDVNRTISYTFIIGSVLAAIGGVLIASH
                     IGRINFYIGFIAGIKAFTAAVLGGIGSIPGAVLGALILGWTESFATGYVSSDYEDVFA
                     FLLLVIILIVRPSGLLGRSTVQKV"
     gene            21449..>21974
                     /locus_tag="PLT63_04000"
     CDS             21449..>21974
                     /locus_tag="PLT63_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97920.1"
                     /translation="MPAIAQIRQSLIIALWFMFLTFPILVVRVNTIENIIEWRWLNMM
                     YVGAGSFLGSFAWRYFLGRRERSASRNAVPAGTRQLAAVAVGPGYDVGPAGIALAGGV
                     ETGRSGGVEEKVSTPPAPPAKPWLHRLMDDRKLRRPLLLILAAAGTIFPFLPFASTYQ
                     INIVTNALLYVMLGL"
     assembly_gap    21975..22074
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <22075..22962
                     /locus_tag="PLT63_04005"
     CDS             <22075..22962
                     /locus_tag="PLT63_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOD97921.1"
                     /translation="TYQINIVTNALLYVMLGLGLNIVVGLAGLLHLGYVAFYAVGAYS
                     YALLYHHFGIGFWTALPLGALLATLFGIALGFPILRLRGDYLAIVTLGFAEITRLVLE
                     NWNEFSFGPSGIAGIPRPGLFGWEMDLHGATIYIYFLMIFLCLLTIFVVNRLQNSRIG
                     RAWVALREDETACEAMGVNPTAAKLTAFALGSTWAGLAGVVFAAKTTFVNPASFTFLE
                     SAMILSIVVLGGMGSILGVIIGAFVLILTPEYLRAFSEYRMLLFGLIMVMMMIFRPQG
                     IVSGVRRIYEFHRGPGEQR"
     gene            22959..23759
                     /locus_tag="PLT63_04010"
     CDS             22959..23759
                     /locus_tag="PLT63_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD97922.1"
                     /translation="MNAVTEPLLNVQTLTMNFGGLRALDQVSLQVQPGEVVALIGPNG
                     AGKTTFFNCVTGIYRPSGGDVFVTPPGKKRRRLNGLKTHQVTASGLARTFQNIRLFQE
                     MTVLENVMIGRHCRTRAGVLGAVLRDPQTRREERDIVERSYTILEKIGLAGEVNELAK
                     NLPYGDQRRLEIARAMATEPFLLLLDEPAAGMNPRETDALEALIRRICDEEGIAVLLI
                     EHDMRIVMSISNRIYVLDYGKKIAAGTAAEIWENPVVIKAYLGEETEE"
     gene            23952..>24664
                     /locus_tag="PLT63_04015"
     CDS             23952..>24664
                     /locus_tag="PLT63_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD97923.1"
                     /translation="MSGLLELIDVQTYYGNIQALKDITLTVGEGEIITLIGANGAGKS
                     TTLMSISGIVPPRAGDILFMGRRLGGVSPNEIVALGISQVPEGRRIFPYLTVTENLDM
                     GAFLRKDQAGVKKDMEHIFALFPILAERRNQPGGTLSGGEQQMLAISRALMARPRLLL
                     LDEPSLGLAPIYVRLIFDIIRRINREDGTTIFLVEQNANMALKVAHRGYVMETGRIVM
                     ADTAENLRNDEGVKRAYLGA"
BASE COUNT         4689 a         7233 c         7614 g         4828 t
ORIGIN      
        1 atcagggatc ggcccgtcat gagcggggag ggggggagcc ccaggagatc caggaccgtc
       61 ggcgccgcgt cggcaagctc cccctgcctc cgcagggtta tccgcccgtc cggggagatg
      121 agggcgaagg ggacgggact gtccgtatgc ccggtgtcca cggcgccgtc ggggtagagc
      181 catttctcca ctgtgccgtg gtcggcggag acgacgaccg tcatccccgc ctcggcggcg
      241 gcggcgatgg cggcgcccgc ctggaggtcc actgcttcga tggcccggat gatggcctcc
      301 cggttttcaa tgtgtcccac cacatccaca ttgggaaagt tggcaaagat aaaggaatag
      361 cgctggtcgt ggatcgcgtc cgcgatcgcc gccccgatct ccccgatgga catctccggg
      421 gcggcatcga aaagggcgac gtccttgcgg gtcggcacca cgatccggtc ttcgccgggg
      481 aagggctcct gccgcttgcc gttgaggaaa tagctcacgt ggaccgcctt ttccgcctcg
      541 gtgatcttca cctgcttcag accggcccgg gcgatcaggg cgctcagggt attctccaga
      601 gtctcttcgg ggggaaaggc cacctccacg ggaaggtccc gccggtactc gatcatggtg
      661 gcgtaggcca gccccagatc cggggcggtg gggaattcgg cgaagccctt ttccgtgagg
      721 ctcctggtta gttctatttc ccgttcgccc cggatgttgt aaaagattac ggcgtcgcgc
      781 ttgccgatcc tgcccaacgg cgcgccgtcc tttcctccgg cgaccagcgg ctgcagcgtc
      841 tcgtcttcct cgccggcccg gtatgactcg cggaccgcct cggacagcgg ataattcccg
      901 gttggttcca taatgacgat ctccccgtgt ggatgtcgcg gccgtccgtt cctgtctgtc
      961 gggatgtccg gcggtccgct gtttttcggc ttgttggccg ggacgggcca agggtgccgc
     1021 ccgcccgtga cttgtataga aaagcgccgg gtctgtaaag gaaaaatcat aacaatgtcg
     1081 gctattccct ggttcgggtc gtgcttttta attgatcgtc aacaacttaa gtgatagaat
     1141 accttatatg aattaacatc gggaaggcat cggcggcaag atgaagggaa aaacgattga
     1201 tgatatcctt cgggaggaat tcctcaaaga gcgggccgtg gttctggggc ggaccgggga
     1261 gagcgtggcc cgggccattg ccaggctcca gcatctggcg cagatcatag agaatcgtca
     1321 tcaggagctc cggcgcctgg accgggagcc ggacggcgcc acggaatgcc atgaactgag
     1381 gacggcgaaa gaaagcgccg taaacgatat caacagcaag atcgcccagt acaaccgcct
     1441 ctgggaccac gcccagttgc gctactatta tctcatcgtc accagggagg ccatggggtt
     1501 gcgaagccat caccgcgtgg aggagatata tcgcattcca ccgaagaaaa aacagctttc
     1561 aggaggataa tggaccgatt ccagaagcta cggatcaaga tggtggagac ccagatccgg
     1621 gctcggggcg tgaccgacga aagggtcctg gccgcgatgg ccaaaatacc ccgccatctt
     1681 tttatcgacg aagccctcat cgaacaggca tacaacgaca accccctgcc tatcggaaag
     1741 agccaaacga tctcgcagcc ttacattgtc gccctgatga cggcggcgct gaagctgacg
     1801 ggaaaggaaa aggtgctgga gatcggtacg gggtcgggtt atcagaccgc gatcctggcg
     1861 gaactggcgg aacaggtctt ttccatcgag cggattgccc agttggcggc gggggcgagg
     1921 aagcgactgg atgccctcaa ctgcttcaat gtggccatgc gggtcgggga cggaacctac
     1981 ggctggcggg aggagtctcc ttttgacggc atcatggtca cggcgggggc gccgaaggtt
     2041 ccgacaatcc ttctggaaca gcttgccgtc ggcggccgcc tcgtcatacc gacaggagga
     2101 cggataagcc aggagttgct cagggttacc cgcatgtccg ccgatctgaa tgaggtcaag
     2161 acggaggtcc tgtgtggatg ccgttttgtc gatctcattg gcgagcatgg ttggaacaat
     2221 tgagcatgcc tcccgacccc gccgtcttga agaaactcgc catgagatat cttgctgtat
     2281 tgatggccct tctgagcctg ttagtttttt cctgcgccgg tcaaaagaat gtcgtccgtc
     2341 cgggtaaggg ggtgtatcat ctggtaaaaa agggggaaac cctctcgcag atagccctgg
     2401 cctatggggt gagtcaacgg aagctggtgg aggcaaacaa tatccgtcac cccgatcacc
     2461 tggaggcggg cagtgtagtc ttcatccccg atgccgtccg ggtgctcgat gtcggcaagg
     2521 ttttgcaggc gaaggcaacg gtagatcagc cggaaaaggg gccggcgaag aaaagcgggg
     2581 agaaaaaacc gccgtccgta gcggggaaaa aggagcgtaa acccgcggaa ttaccccggg
     2641 agggcacgcc caagccaggg gttgatcatc ggaaaggggg ggcgggaaag ccggctaagg
     2701 aaaagaaccg ggacgccgtt cccactgccg ctcccccgcc gccgcctctg ccggggacag
     2761 cggcgagcgc gtccccgggc aaggtttcct tcgcctggcc cgtgcggggt cgcgtagcat
     2821 cccgtttcgg gaggcagccc aacggaatgg tcttcaatca tattcggatt gctacccggg
     2881 atcacgccca gattgtcgcc gccgcctcgg ggacggtgat cttctccgct cccctccggg
     2941 atttcggaga gacggtcatc atcaagcatg acgatgattt cgccacggtt tacacccatc
     3001 tgggcagcag gtccgtccgg gcggacagcc gcatcaagaa gggggagatn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng acgatatccg gaatgcccgg atgatcgccg
     3181 atgagatccg ggccctgtcc tggaaactcg gcggcggagg tcgggtgacc gatgcggtgg
     3241 tccggcgcgt ttacagcggc ggcaacggtt acaacgacgc ctttcgctcc gtttgcgcct
     3301 ggttgtactg gggaggggag tcggtttttc tccaggacgc caactccctg attctgaaga
     3361 ccgatgaact ggtggagatc ctctcctttg tcaaggagca gtttcccggc gtcaacagga
     3421 ttacctccta ctgccgctcc aagacagcgg ccagaaaaac cgtggcggag ctggagaaga
     3481 ttcgcgaggc ggggctggcg cggattcata tgggcatgga gagcggctac gatccgctcc
     3541 tcgaattcat tcggaagggc gccacggtgg ccgaccatgt cgaagggggg aaaaggatcg
     3601 tcgcggcggg catttccctc tgcgaataca taattcccgg cctgggcggc gagagctggt
     3661 ctcgggagca cgccgtcgcc accgccgcgg ccgtcaacgc catcaatccc gactacgtgc
     3721 ggttgcggtc ccttcaggtc gtcaagggaa cgggccttta tgagcggatg cagaacggtg
     3781 aattcacccc tcagggggac gaggagacgg tgcgggagat ccgcctgttt atcgaaaacc
     3841 tgacgggggt gcggacaacc atcgtcagcg atcatatcct caacctcctg gaagaggtgg
     3901 agggaaagct tcccgacgac cgggacagga ttctcgccgt catcgatacg tatctgtccc
     3961 tgccggaaca ggatcggatg attttccgcc tgggtcggag gcaggggatc taccggcgtc
     4021 tggccgacct gaacgacccg gtcatgtacg ggcggctgaa ggccgttacg gatgaatacg
     4081 taccggacgg cgccgaacgg tttgacgccg acctgaagga attgatgcat cattacatat
     4141 gatgacgtat tgactatggt ttaaaaatat gttaacatgc gaaattcaaa atatatttgg
     4201 tggtggcaag atgtttaaga ttggcgacat ggcagtatat cccgcgcagg gcgtcggggt
     4261 cattgaaaac attgaaaacc gcgaaatcat ggggaccaag cagacctttt acatcctcaa
     4321 gatcatgggc aacggcatga agatcatgat ccccactaag agtgcgaaat ccgtagggct
     4381 ccgcgaggtc atctccgaga aggagatttc caaggtgtac gatatcctga aaaataaaga
     4441 agttacgatc gacaagcaga cgtggaacaa gcgttaccgg gaatacctgg agaagataaa
     4501 gaccggttcc gtatatgaaa tcgcccgggt cctccgggac cttctcatcc tgaaaaacga
     4561 caagaacctc tccttcgggg agcggaagat gatggatacg gccaagaacc tgctcgtcaa
     4621 ggagatatcc gtggcgacga aatccgatga aaccaagatc gaggaggacc tgaagacgat
     4681 cttcacgatc cagtaaaccg gatcattgcg taaaaagaag catgtttgga agggcggacc
     4741 ggtcctgacg acggcgccgg ccccgcccga ctcgcttcgg ggccggatca cgggcccgga
     4801 ggttgaaaat atccgcccgt aaggcgggta ttttcataat aaatcgaaaa agaaaggagg
     4861 tgaaaaagga atgatagtca gaattatact tatcgccgcc tgttccatta gcggtttctt
     4921 catcgcctat ctaacgtatc cccccagcca gtggtggacc tggcccatag ggttcgtcat
     4981 cgggctggtc attgcgttct tcgtcatcaa ggtggaacag gacattcgta agttttccct
     5041 ccggatcatc ctggggggag tggtgggcat gatcgttggt cttatcatag cgtttcttct
     5101 tgcctatggc ctaaatttcg taagtaatat caagcataat caggaggtcg taccgtggat
     5161 atacgccctc ttgacgggaa tcatgggata cctcggattg gttctcggat cgaaaaaaac
     5221 ggaggaattg aatatattcg gcttcggaca aaccaaggag ccggatgaag ccagagtcct
     5281 ggataccagc gtcatcatcg acggccggat tgccgacgtc tgtgagacgg gctttgtcga
     5341 aggcaccctc atggtgccga gattcgttct cgacgagttg cagtatattg ccgactcttc
     5401 cgacaccctg aagcgttccc ggggtcggcg cgggctcgat atcctgaacc ggatgcagaa
     5461 gatggccggg atcacagtgg aagtaggcga ccaggatttt cccaaggtca agggcgtgga
     5521 cgccaaactg gtagcgttag cgcggaagat caatgccaag atcatcacca acgatttcaa
     5581 tctcaacaag gtggcggaac tgcagggcat caagatcctc aacgtcaacg aactcgccaa
     5641 cgccctgaaa ccggtggtgc tgcccggcga aaccatgacg gtgaagatca tcaaagaagg
     5701 caaggagccc ggacaggggg tggcctattt ggatgacggc accatgatca ttgtcgataa
     5761 cgcccagaaa tttcagggcg ccgttgtaga tgtggcggtg accagcgtcc tccagaccac
     5821 ggcggggcgc atgatctttt cggagatgaa ggccctggcc ggggacaaga aacccttttc
     5881 cctgtcggtg aagtcatgat cgtttcccag gcccggagga ccttgacctc cgggcttttt
     5941 tatatctgaa acatgatgaa ttcgtaaaaa gtcgtcaaac atgcgttcgc tccccacggc
     6001 ggggtctccg ggcgcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnttcgt
     6121 aaaaagtcgt caaacatgcg ttcgctcccc acggcggggt ctccgggcgc atgatgattt
     6181 ccgaattcgg gcatgggggg cttgggaagg tgcaggccac ggctatcatc cccgccggcg
     6241 ggagcggccg gcgcatggga ggcacggaag cgaagcagta cctgctgctt caggggattc
     6301 ccctgctggt ccataccctc cgggtgtttc aggaggcggc ggtgatcggg gagatcatcc
     6361 tcgccgtacc cgccgccgat gtagatagaa tccggaggga aatcctggac cccttccgat
     6421 tgaccaaggt tcggcacgta gtggcgggcg gccggcaaag gcaggactca gtccataacg
     6481 gcatcgcggc cttggccgat gattgcgagc tggtcgtcat tcatgatgcc gcccgacctt
     6541 ttgtcacgcc ggacttgatc gcccgggccg tggaaggcgc ggcacaatac ggcgccgtgg
     6601 ccgcgggcat cccggccagg gataccatca agtattgcga cgccgcgggc cgggttgccc
     6661 gcaccctccc ccgggagcgg atctggatcg tgcagacacc ccaggccttc cgccgggaga
     6721 ctattgtcgc cgctcaccgg caggcccgcc gggacaattt ttccgggacc gacgacgccg
     6781 ccctggtgga gcgtctgggc gtcgcggtgc ggatgatcgt gggcgagtcc gaaaatataa
     6841 agattaccac ccccgatgat ctggtctggg cggagcatat cttgtcggcg cagcgatgat
     6901 tcgctatgat aaggctcccc gccgccgcgc tccggaggcg ggggccggcc ggccggcata
     6961 tatccgccgg gttcgggata gcctccgccc gtaagcgatg ggaatgccgt tgaagaaccc
     7021 gaaggaaaga gataatgaca ggtagcggtg cagggggaat aaaaacggcc cgcgtgggca
     7081 tcggttatga cagccaccgc tggtgcgacg gccgtcccct tgtcctcggc ggggtggaga
     7141 ttccccattc tcggggactc tccggccact ccgacgccga cgccttgatc catgccatct
     7201 gcgatgccgt ccttggggct gtgggcgccg gggatatcgg tgcccatttc cccgacaccg
     7261 atcccgctta ccgggggatc tccagccttg tccttctggc ccgggtaagg gagatcgcgg
     7321 ccgcccgggg aggccgggtg gtgaacatgg atgccacggt gatcatggag cggccgaaat
     7381 tgcgcgccta tatcggcggg atgatcgcca atattgccga cgccctggcc atggatgccg
     7441 ccgcggtgag cgtcaaggcc aagaccaacg aggggatggg tttcgtaggc cgggaggaag
     7501 gggtcgccgt tatggccgtg gccctggtgg agacggccgg ggcgctctga ggagggcggc
     7561 aatggggaat attcccttca tttttggcga gtatgacgat atgatcatgt ggatgaaaaa
     7621 gataaattca cccgaatcag ccgcgggtgc cgcttcatga aggatctccc cccccgcgtc
     7681 cgcttcgccc cttcccccac cggggatctc catgtgggca acgcccgcac cgccctgttc
     7741 aactggcttt tcgcccgccg ttacgggggg gtcctggtcc tgcgcatcga ggatacggat
     7801 caggtccgga cctcccgggt gttcgaggag catctcctcg ccgacctggc ctggctgggc
     7861 atcgactggg acgagggtcc ggggcgcggg ggtccctgcg gaccctacca tcagagtgag
     7921 cgactgcctc tttatcggga cggcctggaa agactcgtcg ccgccggtat ggtttatccc
     7981 tgtttctgta ccgacgagga gctggaggcg gagcgcctca gtcttctttc gcggaaactg
     8041 gccccgcgct attcgggaaa gtgccggagg ctcgacgatg atcaacggcg acggttggcg
     8101 gagtcgggac ggccggcggc ctggcgtttc catgtcccgc cgggaagcat cgccttccgc
     8161 gacctgatcc ggggggagat gcgctttgcc ggcgaggccc tgggggattt catcatcgtc
     8221 cgttccaacg gcatccccgc ctataatttc gccgtcgtgg tcgatgatca cctgatgggg
     8281 attacccacg tgatccgggg tgaggaccac ctttccaaca cggccctgca gctccttctc
     8341 taccgggcgc tgggtttcga gccgccgcga ttcgcccatc acgccctgat tctcgggcgg
     8401 gaccgggcca agctcgggaa gcgccacggc gccaccgccg tccgggaatt ccgggaacag
     8461 ggggtgctgc cggaggccct ggtgaattat ctggccctcc tggggtcctc cttcgaggcg
     8521 gggcgggagt tttgccgggt ggaggagacc atcgcctcct tttccctgga ccgggccggc
     8581 aagagcggcg ccgtcttcga tgaggacaag ctgtggtggc tcaacagcct gtatatcagg
     8641 caggaagccc cggagattct cgctcggcgc ctgcgaccct tcgtcgcggc ggcgggttac
     8701 ggggagattg gcacctctcg cctccacggg atcatcgcag cggtccggga caacctccat
     8761 gacctgacgg aaataggaga ttatttgcga atcttcgacg acgacgccta cgatttatcc
     8821 ccggaggccc gggcgatcat tgccgatccg gaggcccggg aggtgatcgc gtccctcctt
     8881 gacctccttg acggtcccgt tgccgacgtt cagggcgttc agggcgatgg gggcccggag
     8941 gcccaaagcc gcaacgctgc ggcgtccgca accggtgccg gggaagacgc cgggatcgcc
     9001 ggagccaacg atgaggtgac gccaagcctt tatcgaaccg ttatcgagct cctggccgag
     9061 cgcacgggcc ggcgggggaa aaaactgttc ctgcccctcc gcgccgccct cacgggccgg
     9121 accagagggc cggaactgga tcgctgtttc tccgtcctgg gccgggcatc gctacgccgc
     9181 cgcctggaaa gggccctggc ggtctgatcc gggggggccg gtattggccc gtttcgggtg
     9241 ccgggcgggt aaatcaggcc gggacgggag cggtttacag ctccggcgcc ggctttgtcc
     9301 gggccacgga ggacaggagt ggcaccaata aatccgggcc gttataagac agaatgaaac
     9361 ttaggattat tgaagaatcg gtgctgtttg tcagcatcct gaaatggatt gtgcttgcgg
     9421 ccggtatcgg atttgtcgtc ggcgtctcca ccacggtgtt tctcaaaatc ctagcggccg
     9481 ccatagcctt cgcccaggat ttcccgcgtt attaccttgc cctgcccctg ggactcttcg
     9541 ccagtgtcct gatcatcaag tacatggccc gggaagccca gggctacggc acggaaaagg
     9601 tgatcgaggc ggtccacaaa aacggcgggg ccatgcgggc cgtggtcatt cccgcgaagc
     9661 tctgcgccac ggtagtgacc atcgccgccg gtggttccgc cggacaggtg ggaccctgcg
     9721 cccagatcgg cggcgggctg gccgccgcct ttgccaatgc ctttcgcctg gatgccaatg
     9781 accggaaaaa actcgtgatc tgcggcatca gcgccggttt tgccgccgtc ctcggggcgc
     9841 cggtagccgg cgccgtcttc ggattggagg tcctcttcgt cgggtcgctt ctctatgaag
     9901 tcctccttcc ctccttcatc gccgggatca tctcctatca caccgccgcc gtcctgggga
     9961 tcgattattt ccactatccc gtccatttac agccggtttt caccgaggtt ttcttcctcg
    10021 agatcatcgg cgccggcatc tttttcggcc tctgcgccat cctggccatc gagtccctgc
    10081 ggctgacgcg aagtctcttc gacaagattc ccgtctggcc gcctctcaag gggattatcg
    10141 gcgggatttt tctcatcgtg ctcgccttcc tgacgtcctt ccgctacctc ggcctgggta
    10201 tggaaatcat cgccgactcc ctccaggggg taaaggtggc ggcctccgcc ttcgtcatga
    10261 aaaccctgtt taccgccgtg accttcgcct ccggggggag cggcgggatc atcgcccccc
    10321 tgatggtcat cggcgccgcc gccggggcct tcttcgctgc tatgacgggc cttgatgcgg
    10381 ccacctttgc cgcggtggga ctggtgagcg ttttggctgg ggcggcgaac acccccatcg
    10441 ccatgagcat cctggccatc gaactcttcg gaaagggcat cggcgcctac gccgccatcg
    10501 cctgcgtcat cagtttcctc atcaccgggc atcggagcat ctttcccacc caggtcatgg
    10561 cgatgaagaa atcttcgtcc gtggaagtcg aactcggcca ggaactggac aaggtgcagg
    10621 cgcgctacaa gtttcgcgaa aagagcgtta tcggtacggg aattaagatg acgaaaagga
    10681 tcaggaaacc gggaaacggc cggaaagatt gatgccccgg aaatggacac tggcggtgat
    10741 ccatttccgt atccggatca gggaagtttc caggtggatc ggagccgccg ataggtggtt
    10801 tccgggagtt ggatgagaac ggggaaggcg gccggatcga gccagcggag gatcatttcc
    10861 atattttccg gggacatggc cgtgcatccc gccgtgcctc tatcggggcc ttgccaaata
    10921 tggatgaaga tgcaggaacc ccggccgggg atgggtggga cggcgttgtg gtccacgatg
    10981 acgccgaggc ggtactgctg gtcggggcga tgcatttctt cacaacttga ccagtcgtcc
    11041 tcgacgttgt ctatatgtac cagtcgattg taatagcggg atgtgacgtc gtctatgcac
    11101 cttaggctcg gcgtcgcttc ctggtacggc atctttatcc accggcattc ttcgaccggg
    11161 gcgtatccga aggcaccgct caggctgaag atgcccgccg gggcccgacc gtctccctct
    11221 cgtttaaccg gggcccgatc gtttgccttg ccattttctg agccccatcg atgccccttc
    11281 cccgccatcg gggcggactc attgtctgta agtcgttttg aagcttcttt ccgcgcggaa
    11341 aaggatgtgg ccgcgccttc cggatgcagg cccatacccc agcccaggcc gttgcggcct
    11401 actgacaccg ggaccgcccg gcctacgggc cgccatttgt cttgccgtaa agatctttcg
    11461 taacgttgca ttgttccccg tagcgagagc caatcggcgg tcttgacgac tatggcctgt
    11521 cgggacgcca ccgccggggc gggggcagga cggccttcgc cgccgggaga ggcgccgggg
    11581 ttttttgcgg gctcagtcgc ccaggaaaag gcggaaaaca tgaccgtaag cggtgcggct
    11641 ccccacggtg taaaagttag cgacaggata aacagtgtca ataacaataa aagcttccgt
    11701 gaacacatca tcaggacctg ccccggcaat gggtcggtct atgtatgcgg accgattagc
    11761 gccgatgttt tttgcatata ctcccggtcg tttcatgtca agaaggggag gggcgttccc
    11821 ggattccatt cacggtccgg tttccaagcc gcccgcccac ttgcggtacc ggatcagcag
    11881 cagcaggccg ggggccgcca gggcggtgca gatgacgaag aaccagaccc agcccacggc
    11941 gacaacgagg aaacccgtcg gcgccccggt gacataacgg ctgacggcca tgagactgct
    12001 caggagggcg tactgggttg ccgtgaagcg ggtgttgcag agccccatga gcagcgccgt
    12061 ataggccgcc gtccccatgc ccccgcagat gttctcgatc ccgatgaccg tgcttaaggc
    12121 ccagagctgg cggcccgtga actccaggag gacgaaggag atcgtggaga cgccctggag
    12181 gatgccgaag accagcagcg accgccgcag gtcccatttc gtcatcagcg ccccgcccac
    12241 gatgccgccc aggatggtgg cggccatgcc gaaacccttg aagatcgttc ccaattccgt
    12301 ccgggaaaat ccgatatgtt ggaggatgta gggagtggtc atctgggcgg cggcgacgtc
    12361 gccgagcttg tagatgatga cgaacagcag gatctccacc gccccaggcc gcttgaaaaa
    12421 ctccacgaaa ggctgcacga ccgcctcctg gagactttgg ggcggcgccg caccgtgccg
    12481 gaggggccgc cccgcctcgg gatcgggaca gaggaaggtg gcggcaacgc cgaccagcat
    12541 gaccaagccc atgatgacat agacgctccg ccaggagata tgatccgaca tgatcagggc
    12601 cagggcgccg ctgaccagaa tggcgagacg gtagcccatg atccagatcc cggcgccggg
    12661 gccccgttct tccggggcga ggtattccgt ccggtaggcg tccaggacga tgtcctgact
    12721 ggcgctgaag aaggccagcg cgacggccca gaaggcgacg atcaacggct gggaggcggg
    12781 actgaaaaaa cccatggtgg caatggtggc gatcagggcc ccctgggtga tgagcatcca
    12841 tccccgccgc cggcccagaa aggggggaag gaagcgatcc agcagcggcg accagagaaa
    12901 tttgaacgcg taggggagac cgacgagaga aaagatcccg atcgtcttga tgtccacgcc
    12961 ctccgtgacc atccacgcgg cgagggtgga gctggtcagg gccaggggca tccccgaggc
    13021 aaagcccaga accagcatga ggagcatctt tttggtgaaa tagatccgga agagggggaa
    13081 tatgtatttc atgatcgtcc ctgattattt acgcctccgg taacgtgacg ccgcctcgac
    13141 caagacaaga aaaagacgcc gatgcggtgc ttcttcctgc atccgctcgg gatgccactg
    13201 aacgcccagg acaaaacgcc ctatctccgc ccaggccgaa ccgggggccg actcgatggc
    13261 ctcgatgatg ccgtcggcgg tccaggccga ggcgaccagg tcttttccca cccgctccgg
    13321 atggacggcc tggtgatgga aggagttggc ggccaacgcc tcccccggcg gcgtgccggt
    13381 ggtacaggtg gcgaggctta acggcgcaaa gcgcacggaa tgcaggtagc agtccttgct
    13441 gccttccgga tgaactctcc ctggggacgc cgccggccgg ttgctgcttt ctcccgttgc
    13501 ggccttgtcc gccttgtcgg cagtcggacc gttggccata cccttctctt ccccggcaag
    13561 agcgccggtc cggtgggttt ctgcttcctt cgtttcctcc gccggggtcg tcgccgtgat
    13621 caaatcggga aagtccgcag gcgcttcgtc ccccacgccc cgctcccggt cctttgcgct
    13681 gtggggtagg gacggaccgc ctcccggcgg ccgccattcc gttccgatgt cctgaatcaa
    13741 cgcgccgccc cggatgatgg acaagagctg gcagcccccg cagatcccca ggaccgggag
    13801 atccgtctct tccaggatcg ttttggccag ggcgacgtcg aaacgatccc gccggggatg
    13861 caccagctcc tccgccggct ggggatggcc tccgtaatgc tctgggcggt aatcggcccc
    13921 gccgatgaag agaaagcccc ggagccgggg gatgatcttc cggatcgtcg ctaaatcttc
    13981 gccaggaggg atgcagaggg ggacgccgcc ggccccggcg accgcggcta cgtagtccgg
    14041 ggggacgaac aggcgctcga ctccggaggc ggccgtgctc tctatggaca tgttcaaacc
    14101 gatcagcggc ggcacggttc aatttccgaa gcggcgattg tagaggtcgg caaagccgtc
    14161 cttgcccgtg gccataagca aggttaaagg catggtcttc tccgccgccg ccagcatcga
    14221 cgccgtgagg ttccggatat cccactgggc gtggcggtcc gcccgaaggc gggcgatatg
    14281 atagagttcc cgggcgttga ccttcagggc gacgcgccgc cggtgggcgt tggtcaggat
    14341 gtaggaggcg gcctcagcgg cctccgtctc ccggagctgt tcgtaccgct taccggtgaa
    14401 gtccatgagt ttccggaaag attcgtccat cccgatggcg gcaatcgatg gcgggatggt
    14461 gacgcccaga ccggggtcgt atccctgggc ggtgatggtg gccatgcggt ggcgtttcaa
    14521 ctgggcgaaa caaccggcgc tgacggtgag ttcgaagagg agatccacat attcgaattc
    14581 tctcaggacg gtatcgtgag accgtaggtg acgcagggcg gttttgacga ggttcatacg
    14641 ttgcccggca tccagtcgcc gggcccggga aagacaggaa gacaggggcc gccgggacga
    14701 ggaatggagg agggccgcca ggagccggag atcggcttcg ggcgtgacat aggccaagaa
    14761 gacgtcgtct cgcgacgctt ccggcaatgt cgtcaccggg gcgatggcgt cacccctttg
    14821 ccctgggggc gtttgaacca cctgctccac ctcgggaccg ccgccgtcgc ctgtcatccg
    14881 ccgatcccgt tcgtccggcg agtcttcctt gaacagggcg gccgccgcct cccgcaactc
    14941 cctccgtgtc agcagatcgt agtccgtcgc ctcggtatag cgcaccaggg acggggcgat
    15001 ggacttggtg gccttataga gggcggcgct caaatcccgg gcctccttta agggatgagc
    15061 ggcgaggcgg cggagcatga gttccagatt ccgggcgttg agggtcatcc ccagttgggt
    15121 ttccgtggcc agggcgatgg cgtagcgggc atcttccttg gcccagcctt cgagaaggga
    15181 gcggttcgcg ggatcccgcg ccagctccgg atgccgggcg aagacatggg ggcggagctg
    15241 ttcgtagcat tcccggtaaa aggagttctg acgcccgacg acctcggcga acggccctgc
    15301 cagccccgca ttttttatct cctccgggat cacgaagtcg tccttgaaca ggacgtagcg
    15361 ctgagatttt tccgtatagg aacagaggcg aaacttctcg atgtcctcca ccaaaagccg
    15421 ggataccccc aggacgtcga ggttgaatac cgcgtgttcg gcgatggaac tgtggcccat
    15481 ctcgaagacg atattccggt ttgactgacg ggccttgtcc acctctcccc gggcggcggc
    15541 ccgcagttcg tgcaccggcc ggggggagcg gctgatccgg gcataggcgg cggcgatggt
    15601 ttccggcgtc agttcttcga ggtcctcgca gcggtcctcg atgtccatga tcgtttcgta
    15661 atcgatgttg taaccggcaa gaatgacttt catatgctga tcaacgaaat ttgaagtttt
    15721 tatctttcag cagcctcaga caggcagcca cgacgtcggc gtcgtaacgg acgccctgct
    15781 ggctgctgat ttcttcgatg gccttgtgga tccccaggga cggccggtac gggcggtgtg
    15841 aagccatgga ttccaccacg tccgccacgc tgattatcct cgacgccaat agtaaatcct
    15901 gacctttcag atgccgggga tagccggagc cgtccatccg ctcatgatgt tccatgatca
    15961 tccgggctat cggccaggga aaatcgatgc cctggaggat gtcgtggccg atctgggcat
    16021 ggaacttgat gaggtcgtat tcgatatccg tcaaccgcgt cggtttgctc agaatttccg
    16081 ccgggatgga caatttgccc agatcgtgga tcgccccggc cacccggatg ccgtccgcct
    16141 gctccggcga caggcccatt tccagggcaa tcgcccgggc gagttcggcc actcgcttct
    16201 ggtggcccgc cgtataaggg tccctggctt cgacggtggc ggatatgacc tggatgatcg
    16261 cctcaatgga tttctccatc cgggcgatag tctgcccgat gagatcttcc gcttccctgc
    16321 gggcggtgat gtcggtaaga aaattcagcg tcgccccccg gccgttccag gtaaaggaga
    16381 cggcgttgat ctccacccag atgatcttac ccgtttttgt cacgcagcgg aaggggtaga
    16441 tctccggata agcggatccg ccggccaccc ttgcttggaa acgttcgctt accatctccc
    16501 gatcctcagg atggatgcag tccgtgaacg gacgggaaag gagttcttcc gcggaatatt
    16561 cggtcatttc catcgttttg ggattggcga acttgatgac cccggactgg gcgacgacga
    16621 tcgcctcgtt ggcattgttg atgaggagac ggtattgcgc ttcgcttttc cggatggctt
    16681 cttcagccag gacgcggtcc gtgatgtcga tggccgttcc cagtatggcg ggacgccctt
    16741 cgtattccag attggtggcg gctaaatcta tccaccgttg ctccccgttc ttggtgacga
    16801 tcttcaactc ataacgggac ggaactgctt ccccccgctg ccgggccaga ccccggacct
    16861 tgatgagttc ccgataatcg ccatgcgcca gttcccaata atcaatccac ttcaattccg
    16921 cgaggttgta accggtgatt cgttcaaagg cgagattgac atagcggact ttcgtgtcct
    16981 ggatgatgaa gatcgccgag ggggtcgtct cggcaatggc gcggaatgtt tcctcgctcg
    17041 ccttcagggc ggaggcgacc tggcgcttat ccgtcacgtc ccttatgtag ccgccgatgc
    17101 cgtacttgtc cttaccgatc cttacccgga attttctggt ctcgtagata cggtcgccca
    17161 cgggctcttc gtcggtgtgg atagtatcgg tctccaatgc ctccgtatcg gacatccggc
    17221 actttctcgc cgccccttcc ggcatgagtt cgaagtcggt cctgccgatc acctcggatt
    17281 cccgcttgcc gaagaatctc aggagcggcg cgttggcgat gatgtggcga aacgattcat
    17341 ccttcaggta ggccatatcg gatatggaat cgagaaaagt gcggtagaag tccgtcaggt
    17401 cgccgtcttc cccggtgaaa atatcgttcc cggcttcgct cggaatgcgg ttgctccggc
    17461 gctctgacga cggtgcggcc gggcttgcgg gaggttctcg gtgctgcggc atgttttccc
    17521 ccgagataat ttagttagag agatttctat atgaaccgcc gatatatttc aagagaataa
    17581 agctttgccc gaaaatgagt tcactccggc ctcaccggac taatggcgcc gttacccggc
    17641 gccggcacgg ttatttcttg acatcgagcg gcaaaaaaac gaaaagaggc aaaatggatg
    17701 acatcgcaat atgtcgtcaa agtgaccggc gcacaaataa ttcccatgcc ccgcggtgcg
    17761 cagggaagcg cgataatcgg atttgacttt ttagcggtgg ctctggggac tccctattta
    17821 gcatttggat ctgaaagcgc cggccaagtt cataaggacg ccattttgcg gggttgtcaa
    17881 gggcgggtcg gccgggcagg agaaggaagg taaatgtacc ggatgaaaat cacgattggc
    17941 ggcgattatc gccggaccgc ggcggtggcg taatgagacc ggtaattgcc gtcatcggcc
    18001 ggcccaatgt gggcaaatcg accctcttca accgcctggc cggccggggc aaggcgatcg
    18061 tcatcgacga gccgggggca acgcgggacc ggaattatgc cgacggcgat tggcagggac
    18121 gaccgtttgt cctcatagac accggcggct tcgagccggc ggctaccgac ggcatcctgg
    18181 cccagatgcg ggaacagacc accctcgcca tggaagaggc ggacatcata gtttttctca
    18241 tggacgggcg cgacgggctg acgccggcgg accgggaaat agccgccatg ctccgggaga
    18301 tcgataaacc ggtcttccat gtcgtcaaca agatcgacgg cccgaaacac gagcaacttg
    18361 cctatgattt ttacggtctg ggagtcgaag agatctttac cctttccgcc cagcatggtc
    18421 agggcgtttt cgagcttatg gatgcgatgg tgcatctcct tcccaaggag gtcgaggcgt
    18481 cgcgggaaga ggagcggctc cgcatcgccg tcatcggcaa gccaaacgtg gggaaatcat
    18541 ccctgatcaa ccgcctcctg ggttatgagc ggaccattgc caatcccatg ccggggacaa
    18601 cccgggacgc catcgacacc ccctttgaac ggcaggggag aaagtatctc cttgtcgata
    18661 cggcggggat ccgccgcaag agccgcgtca gccttaccct ggagaagtac agcatcgttc
    18721 aggccctcaa gacgatcgac cggtgcgaca tcgccctgat cctcatcgat gccgccgagg
    18781 gcattacgga gcaggacgtc aagattgccg gcctggccca cgagcggggt acagcctgca
    18841 tcctggcggt caacaagtgg gatttggtgg tcaaggacaa cgaaacgatc ggcgtttacg
    18901 taaaggacat caaggacaaa gccaagttcc tcgatttcgc gccgatcatc ttcatctccg
    18961 ccctttcggg gcagcgggtg acgaagatct tcgacctgat cgaaaaggtt tacgaacaat
    19021 acacccggcg gatctccacc ggcgatctca atcgggcggt acgggaattt acggaccgga
    19081 atccgccgtc ccggtccggc ggcagcccca acgcctttgc ctatgttacc caggtggccg
    19141 tcaagccgcc aacatttatc ttctttgtgc gcgatccccg ggcggtccat ttctcctacg
    19201 agcgctatct ggccaatcag ctccgggagg ccttcggttt cgactccgtg ccgcttagga
    19261 tcttctttaa gaaaaagagc aaatcccagc tttcatcttg acgagggagg gatgtttaca
    19321 gtatggacgg acttctaacc aagacaacag gaaaaggagg ttatatggtc gtgaaatcat
    19381 ctacgcgtat ctttgtgccc tggttgattg gcgttggttt ggcgttcatg cttagcggtt
    19441 tcgccgccgc ggcggacacc atcaggctgg gtgtcgccgg tgcccacagc ggcgatctgg
    19501 cttcttacgg cctgcccacc aaacgggccg ccgagcttgt ggtcaaggaa tacaatgcca
    19561 agggcggcgt cctgggcaag aaggtggaga tcctggccga ggatgacgtc tgcaaaccgg
    19621 aagtggcgac gaacaccgcc accaagctgg tgtccggcgg cgtccatgtg gtgctgggac
    19681 atatctgcag tggggcaacg aaaacggccc tgggtattta taaagatgcg ggcatcgtca
    19741 ccatgtcttc ctccgccacc aatcccgatc tgacccagag cggcaggtac ccgaatttct
    19801 tccgcaccat cgcctccgat gacgcccagg cccgtctgga tgccgggttc gccatggatg
    19861 tcctgaaggt gaaaaagatc gccgtgctcc acgacaaggg agattacggg aaaggcctgg
    19921 cggaatttgc ccgttccttc atcgaggcct ccaggaaggc caaggtggtc ctcttcgagg
    19981 gaataacgcc gggcgccgtc gattattccg cggtggtgaa caagatcaaa caatccgggg
    20041 cggaggcggt tatctatggc gggtatcacc ccgaggcctc ctccatcgtc atccagatgc
    20101 gcaagaaaag gatgaatacc tacttcatct ccgatgacgg ggtgaaggac gataccttca
    20161 tcaaggtggc cggcaaggcc gcggaaggcg tctatgcctc gggccccaag gacaccacca
    20221 agaatcccct ggccatcgcc gccatcgccg cccacaagaa gacctacaat gccgacccgg
    20281 gcgccttctt cctgaacggt tacgccgccg cccaggccct cctggcggca atccagaagg
    20341 ccaaatccac caagtacgcc gccgtggcca aggcgttgcg cacctacccg gtggataccc
    20401 ccctgggaaa gatcaagttc gacaagaacg gcgacgccat tggcgtgggc ttttccatgt
    20461 atcaggtgaa aaacggggtc tatgtcgagg tgcccgtggc ggccgccccg gcaccgaaaa
    20521 agaagagata agtggattat ttccttgagc tttttttcgg aggactgact cggggcagca
    20581 tctacgccct cattgccctg ggctatacga tggtgtacgg catcatcgag ctgatcaatt
    20641 tcgcccacgg ggagatttac atgatcgggg ccttcacggc cctgatcgtg tcttccgtcc
    20701 tggccttcgc gggcttctcg ggactttcgg tcctcttcct ggcggcggcg gtggcgatca
    20761 tctactccgc cgcctacggc tttaccgtgg agaagatagc ctacaagccc ctgcgccagg
    20821 cctctcgtct tgcccccctc atcagcgcca tcggcatgtc catcttcctc cagaattacg
    20881 tcatgctggc ccagacatcg gatttccttc cctttcccag tctccttccc gaattgccgt
    20941 tcctggagga gctggagctc agcattggct ccacggaatt gatcatcctg atgaccacgg
    21001 cggtctccat ggtgctgctg accctcctca tcaagttcac gaagatgggg aaggccatgc
    21061 gggccaccgc ccaggaccgc aaaatggcca tgctggtagg ggtcgacgtc aaccggacga
    21121 tttcctatac ctttatcatc ggttcggtac tggctgccat cggcggggtc ctcatcgcct
    21181 cccacatcgg ccggataaat ttctacatcg gcttcatcgc cggcatcaag gcctttacgg
    21241 cggcggtctt gggcgggatc ggttccatcc ccggggcggt gctgggggcc ctgatcctgg
    21301 gctggacgga gagcttcgcc accggctacg tttccagtga ttacgaagac gtctttgcct
    21361 tcctcctcct cgtgatcatt ttgatcgtgc gtccttccgg tctgttgggc cgttcgacgg
    21421 tacagaaggt ataagaggag ccgctccctt gcccgccatt gcccagatcc gccaatccct
    21481 gatcatcgcc ctctggttca tgttcctgac ctttcccatt ttggtggtcc gcgtcaatac
    21541 catcgaaaat atcatcgagt ggcgctggct gaatatgatg tatgtggggg cgggaagctt
    21601 tctggggtcc ttcgcctggc gatattttct gggccggagg gagcgctccg caagcagaaa
    21661 tgccgttccg gccgggaccc gccaactggc agcggtagct gtcggccctg ggtacgacgt
    21721 ggggccggcg ggaattgcgt tggccggtgg agtggagact ggtcgctccg gtggggtgga
    21781 agaaaaggta tcaacgccgc cggcgccgcc ggccaaaccc tggctgcatc ggttgatgga
    21841 cgaccggaaa ctccgccgcc ccctgctgct gatcctggcc gccgccggga cgatcttccc
    21901 ctttcttccc ttcgcctcca cctatcagat caatatcgtc accaacgccc tcctctacgt
    21961 gatgctggga ttggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnacctat
    22081 cagatcaata tcgtcaccaa cgccctcctc tacgtgatgc tgggattggg gctgaacatc
    22141 gtcgtcggcc tggcggggct cctccatctg ggttacgtgg cgttctatgc cgtgggcgcc
    22201 tacagttacg ccctcctcta tcaccatttc gggatcggct tctggaccgc cctgcccttg
    22261 ggcgccctcc tggcaaccct gttcggaatt gccttgggat ttcccatcct gcgtctccgg
    22321 ggtgattatc tggccatcgt caccctgggc tttgccgaga taacccgcct cgtcctggaa
    22381 aactggaacg agttttcctt cggtccgagc gggatcgccg ggattccccg gccgggtctc
    22441 ttcggttggg aaatggatct ccacggggcc acgatctaca tctatttcct catgatcttt
    22501 ctgtgcctcc tgacgatctt tgtggtcaac cgtctccaga actcccggat cggccgggcc
    22561 tgggtggccc tccgggagga cgaaacggcg tgtgaggcca tgggcgtcaa tcccaccgcg
    22621 gcgaaactta cggccttcgc cctggggtcc acctgggcgg gtctcgccgg cgtggtcttt
    22681 gccgccaaga cgaccttcgt caatccggcg agcttcacct tcctcgaatc ggccatgatc
    22741 ctctccatcg tcgtcctggg cggcatgggt tccatcctgg gcgtgatcat cggcgccttt
    22801 gttctgatcc tcacgccgga atatctccgg gccttcagcg aataccggat gctgctgttc
    22861 ggcctgatca tggtgatgat gatgatcttc cggccccagg gcatcgtcag cggcgtccgc
    22921 cggatctatg aattccatcg gggaccgggg gagcagagat gaacgccgtg acggaacccc
    22981 tcctcaatgt ccagacactg accatgaatt tcggcggtct ccgggccctg gaccaggtaa
    23041 gcctccaggt tcagcccgga gaggtggtgg ccctgatcgg ccccaacggc gcggggaaaa
    23101 ccaccttctt caactgcgtt accggcatct accggccctc ggggggggat gtcttcgtga
    23161 cgccgccggg gaagaagcgg cggcgcctca atggtctgaa gacccaccag gtcacggcct
    23221 cggggctggc ccggaccttc cagaacatcc gcctcttcca ggagatgacc gtcctggaga
    23281 acgtcatgat cggccgccac tgccggaccc gggcgggggt cctcggggcg gtcctccggg
    23341 acccccagac ccgccgagag gaaagggata tcgtggagcg cagttacacc atcctggaaa
    23401 agatcggtct cgccggcgag gtaaacgagc tggccaagaa ccttccctac ggcgatcaac
    23461 gccgcctgga gatcgcccgg gccatggcga cggagccctt cctcctcctc ctcgacgagc
    23521 ccgccgcggg gatgaacccc cgggagaccg acgccctcga ggccctgatc cgccggatct
    23581 gtgacgagga gggcatcgcg gtgctcctga tcgaacatga catgcggatc gtcatgagca
    23641 tctccaatcg catctatgtt ctcgattacg gtaagaaaat cgccgccggc accgcggcgg
    23701 agatctggga gaatcccgtg gtgatcaagg cctatctggg cgaggaaacg gaggaataat
    23761 atggagacgg tcctcttttt cggcgacaac cctgagccgg cgacaagcaa atgggaaaaa
    23821 cggggacggt tcccattttt tcgtaagcat atcgaactag tcgcaagggg gacggtcctc
    23881 gcttgctgcg gtgatcttca cccggcggca gccgggaatt ccttcctcat gcggagcatc
    23941 ccggaggggc gatgagcggc ctactcgaat tgatcgacgt ccagacttat tatggcaaca
    24001 tccaggccct gaaggacatt acgcttaccg taggggaggg ggagatcatc accctcatcg
    24061 gggccaacgg ggcgggcaaa tccaccaccc tgatgtccat ctcgggcatt gtcccgccgc
    24121 gggccggcga catcctgttc atgggccgga ggcttggcgg cgtcagcccc aacgagatcg
    24181 tcgcgttggg gatctcccag gtgccggaag ggcgccggat cttcccctat ctcacggtga
    24241 cggagaatct cgacatgggg gccttcctcc gcaaggacca ggccggcgtg aagaaggaca
    24301 tggagcatat cttcgcgctt ttccccatcc ttgcggagcg gcggaaccag cccggaggca
    24361 ccctctccgg cggcgagcag cagatgctgg ccatttcccg ggccctgatg gcccggcccc
    24421 gtctgctcct tctcgacgag ccctccctgg ggctggcccc gatttacgtg cgcctcatct
    24481 tcgacatcat ccgccggatc aaccgcgagg acgggacgac gatctttctg gtggaacaga
    24541 acgccaatat ggccctgaag gtggcccatc gggggtatgt catggagacg ggccggatcg
    24601 tcatggcgga tacggcggaa aacctccgca acgacgaagg ggtgaagcgg gcctacctgg
    24661 gggc
//
LOCUS       DAOSMF010000020        23310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_2747_length_23310_cov_22.389250, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000020 DAOSMF010000000
VERSION     DAOSMF010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 23310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23310
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2747_length_23310_cov_22.389250"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1060)
                     /locus_tag="PLT63_04020"
     CDS             complement(<1..1060)
                     /locus_tag="PLT63_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOD97924.1"
                     /translation="MIEERCINAIRFLAADAAEKAKSGHPGMPMGAAVLAYTLWMRHL
                     KHNPADPAWPDRDRVILSAGHASMLLYSLLYLTGYDLTIEDLRSFRQWESKTPGHPEL
                     GRPPGAEMTTGPLGQGVGHAVGMALAEAHLAATYNRPGHEIVDHFTYVLASDGDIMEG
                     VTAEACALAGHLGLGKLVVLYDDNGVSLAGATALTFTEDVGGRFAAMGWQVLEVADGN
                     DVAALDAALAAAKEERWRPTLIKVRTCIGYGAPTKEGTAACHGSPLGKEELAAAKVRL
                     DWPEEPAFYVPEEVLAHCRRPSLERGAARQKAWEEAFRAYAAAFPEAAAEFRRVMAGE
                     LPAGWEEAIPDFSPAARAE"
     gene            1263..1538
                     /locus_tag="PLT63_04025"
     CDS             1263..1538
                     /locus_tag="PLT63_04025"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HOD97925.1"
                     /translation="MSGALESLKVLDFTTLLPGPFATMCLADMGADVLRVTSGSRPDL
                     VEFLPPRLPATGVGAVSAYLGRGKRSLALNLKGPPGDRDRPSAAGGL"
     gene            1546..2670
                     /locus_tag="PLT63_04030"
     CDS             1546..2670
                     /locus_tag="PLT63_04030"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HOD97926.1"
                     /translation="MEQFRPGIMTKLGLDYERLKKVNPRVIYCSISGYGQMGPLRDRA
                     GHDMNYMALSGAMGYSGRKATGPTLMGIQIADIASGSNNAVIGILAAVVSRAATGEGQ
                     YIDISMTDGVMALNAMAGAAYLAGGEAPGREENWLNGGSLYDFYATKDGGYLSIGSLE
                     PQFFRTLCEVIGRPDLIPGGVQPKAGDFERVREEIRAIVRTKTRAEWEAIFKEVDACV
                     EPVLSLAEALEGELARGREMVVDVPLAAPDGSDVGRGDAGGGGGALGAGDGAFAAGVQ
                     GAGKASTIKESGARGTAGAAGGGGASGKAAGDPGAATGGIAAPRATVRQLANPVKFSR
                     TPASYAFAGVKTGTHNREVLKALGYTEAQIAEFAATGLFD"
     gene            2703..2867
                     /locus_tag="PLT63_04035"
     CDS             2703..2867
                     /locus_tag="PLT63_04035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOD97927.1"
                     /translation="MATYEYRCEACKKSFTLIQKMSEHGKAKVTCPKCKSEKVKQQIS
                     GFLIKTSRKS"
     gene            3072..4211
                     /locus_tag="PLT63_04040"
     CDS             3072..4211
                     /locus_tag="PLT63_04040"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HOD97928.1"
                     /translation="MGDPPARTPEDAGAGYRRDQQPSQPGPPPCQTENANFKDLTPYD
                     EDEINLLDLGRVIWKRRRLIGYIAAAMVFLTVVVSLLMTNIYQSEAVIMPVAGKEVGA
                     GGGAAALLAAQFGGLAGIATPGSTSTQEIVSLLKSNVLREKVIEQHKLLPVLFEEEWD
                     AEKKRWKAEGGGFRINLNPISWVSSLLALVRPPPPPSIAKKKPGIPDVWDGIRALDEM
                     VTVKHDVKQGSITITGQFPDPELAARVVENFLTTLTDHMSAEARRVALTNQKYLEGQL
                     SATTDPFIKQKIYNLIAQQVETSMMAEVKENFAFKILDPPRVPDQKFKPKRAQMVMLS
                     FVVALFIGIFMAFFLEFLEKQNIHIDAGRWPLINRLAGWKRRLLI"
     gene            4208..7747
                     /locus_tag="PLT63_04045"
     CDS             4208..7747
                     /locus_tag="PLT63_04045"
                     /inference="COORDINATES: protein motif:HMM:NF018010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HOD97929.1"
                     /translation="MNIRRFFGIGIFILAIASIIGALPAVAQTPNITAQPLPGMMGQQ
                     MPTTVTADVCKNLPASQRAVCEAEIQRSGGRLTPEALQAIKERPAVTGTTPDEAARVR
                     QLTEQKERQKITVEEYKEKTIFDRARNIGKYQDVSTDLKRFGADFFSEAEVSVTVDRK
                     DVPVPMSYVIGPGDEIKLLLWGRVNAQYDLIVDRDGKITIPQIGPLHVSGLTFEQMSK
                     LVIKQAEQIVGANIDISMGSPKMIPIFVIGDVKRPGSYTVGSFSTITDALLTAGGPTE
                     IGSMRNVQLKRKGRLVTTFDLYDLLLKGDKSHDVALLAGDVIFVPITGPVAGIAGNVR
                     RPALYELKHRYDLEYLLELAGGITPSAYTQQIQIERIVRNQRHIVMDIDDKKMAKAAE
                     IALHDADLVKIFPIMDINVNVVYLNGNVKRPGKYEMKPGMRVGDLLKNSDDLLSETYL
                     EYALIKRLEPPDMKTVLVPLNLENLLLKKDERANVELKPRDSIYVFHQGLFKDSPFVR
                     IEGEIRGDCATDKEAETTGEMTRESLAGMRAMTSEPGGGMRTGMTVDTRTAAGGTAAA
                     GAGSEESRYSQYLLSQSRAEKPRYSQYRLSQSLKAIEEDLKKQGLTDLAKQVWTIESE
                     VADNLKIPPEDFRNLRTQLVKVGRPDLANQLLEIEQKVKKGGALADVTAELVLIENIL
                     KRMNMYDQAATVRSIHDEIKNFGRVPTENMRVLRADLIRTNQADLANRLLEIENRTKL
                     SCNIPFVENMRVKDVILISGGLTSNSYLDRAEIIRTNNKRQYQTLFFDLGKAMAGDPQ
                     HNLPLQNEDQIVVHSIWEQVYKKHVAIDGDVARPGTYQYTEGMTVRDLVFKGGNLLES
                     AYLGEAEISSIVVVAGKSSATGRRVINLGRALAGDPQQNVVLMPNDRLLVKRIPNWGS
                     LQYVKLSGEFKFPGKYSFHKGEKLSSVIERAGGYTDNAYLRGAVFKRETVRELQQKSL
                     DEMARRMERELLSESSARVATSISAEEMAAKKQEAELKLKLIEYMKSLQATGRMTIAL
                     SHVRMLKGGAFDIELESGDNLFLPQKTSVVNVVGAVMSEGSHIYNDSLDYKDYVALAG
                     GYARYADEANVFVIKVDGSARKLSSGFATWNDKRDRWEMTAYGKEIKQIEPGDVIVVP
                     ERIAQIAWLREIKDITQVLMNTAVTAATIIKLW"
     gene            7860..8171
                     /locus_tag="PLT63_04050"
     CDS             7860..8171
                     /locus_tag="PLT63_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97930.1"
                     /translation="MKTIGKYTLIVGMIFFMAMLPVSVFAQAQGAGAGTAGAAGAGAG
                     TAGAAGAGAAAGGGALAGIGAGTIAAAAVAAAAIATAAIASSSSKSDSTPAAHSATSH
                     H"
     gene            8358..8816
                     /locus_tag="PLT63_04055"
     CDS             8358..8816
                     /locus_tag="PLT63_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97931.1"
                     /translation="MAPCAAVTQSLSPEAETVLNAAEGVFKAMKARDYPAIWAGLTAK
                     TRNEMVESVRKGAGKTGHEFTAAQLSNDFATGGPQAKAYWDSYLDVFNPDLVLEQSRW
                     SLGSIKGDGATVIIQYRKSENPAILILKKEDGAWRLGLEETFGPRRWIMK"
     gene            8841..10388
                     /gene="radA"
                     /locus_tag="PLT63_04060"
     CDS             8841..10388
                     /gene="radA"
                     /locus_tag="PLT63_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOD97932.1"
                     /translation="MSKKSIDKKPRTAFFCQSCGHDSPKWLGRCPGCGEWNTFVEEEL
                     RETAAGGAGASGRASFSFSGAPQPIEAIEADEGERIRTGMAEMDRVLGGGIVPGSAIL
                     VGGDPGIGKSTLLLQLLQQLAAQGMKVLYITGEESAPQIKLRGRRIGAEAANLFVLVE
                     IDLDSMMRQIEATRPQAVVVDSIQTVCSAALGSAPGSVGQVRESSERLIIQAKKTGIP
                     VFLVGHVTKDGAIAGPKVLEHMVDTVLYFEGDSGHAFRIIRGIKNRFGPTNEIGVFEM
                     RGDGLREVANPSAFFLAERPERAAGSVVVPSMEGTRPILIEIQSLVIPTNFGMPRRTA
                     IGVDANRVSLLVAVLDKVCGLHLSNHDIFINVAGGVKVSEPAIDLGIVSSLASSFLDR
                     AVVPGTVVCGEVGLAGEIRGISRADVRIREAARLGFSRCILPFVAPRGEAGTDAAAAA
                     KPVASSATPAKSTSLSALRSAVPAARAAIASASDRGVASQRGGGQESDIALLPVRSLP
                     ELLSHLF"
     gene            complement(10448..12637)
                     /locus_tag="PLT63_04065"
     CDS             complement(10448..12637)
                     /locus_tag="PLT63_04065"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOD97933.1"
                     /translation="MALNLEAIGKKIGPVTKTYTWKDAALYALGIGAGFDELDYVYEK
                     NLKVIPSFSVAGIFGFFRHVARAANVNLAGILHGEQDIIFYRPIPTGGALTTEGSITH
                     YYDKKDKGAVVIAAGDTCDADGNKLFKNVITMFSRFDGNFGGEEGKTEPFVFPASEPD
                     FVVTAQPSPDQPLLYRLSGDFFQLHVDPDFARQVGFEKPIMHGLCTHGFACRALMEKL
                     TPGQPDLVRRLACRFSKPLYPGEPIRTLIWKTGEGRAVWRTLKAKTGDVVIDNGIFEY
                     GPAAGAQIRFDGRVAVITGAGGGLGRVYALELAKRGAKVVINDFGAARDGAGDGSSSP
                     ADKVVAEIKAAGGEAVANYDNVATPEGGERIVKTALDSFGRLDILINNAGIIRDKGLL
                     KMETENWRAVIDVHLNGAYHVTRPAFAAMRDQGYGRIVMTTSASGLYGNFGQTNYSAA
                     KLGLVGFMNTIKIEGQKYNIKVNSIAPIAASRLTEDILPPDIFAKLKPEFVAPIVLYL
                     VSETCGVTGHIFNAGMGFYNRAAILTGPGTVIGDGRRIPTLEEVADNWEKIGDMEGAK
                     EYFQLMDQVTDLLNAFAPKPKAAAAETTSSDPGSSGGASAIAAAATAGDGTPAQPAPP
                     TVAGIFAQMQQAFQAEKAAGVNVVFQFHITGDGGGDWFAEVKDGACRIEAGKRDAPTT
                     TITMAAGDFLDMMGGKLSAMQAYTSGQIKIGGDLLKSQLIEKLFKLR"
     gene            13385..13714
                     /locus_tag="PLT63_04070"
     CDS             13385..13714
                     /locus_tag="PLT63_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97934.1"
                     /translation="MFQDVAREWIDELTAAGRMENHDEEGRRALRRDYAQRIEEFFIA
                     AVTKQLEPMGKVSDFERMLVWDTQYTHKFLNQTIPGYPSFKREILAKARKAILGDATP
                     AESATKS"
     gene            13728..14492
                     /locus_tag="PLT63_04075"
     CDS             13728..14492
                     /locus_tag="PLT63_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOD97935.1"
                     /translation="MKIIFLGTNGWYDTDTGNTICTLLRTEDYDILLDAGNGLAKADQ
                     YGTGADGRPVYLFLSHFHLDHVAGLHTLTKFSFSGGLTICGPAGSRDILTTLVNQPFT
                     VPLKDLPYPAAVRELPGEVKSLPFPVTALPLRHASLTLGFRLILDGLTVSYCPDTGYC
                     ANAVTLAREADLLIAECAYRRGQGSEDWPHLNPETAARIAGEARAKRLALVHFDARIY
                     PNLELRREAERDARAIFLSSFATFDDLEIELSPSLL"
     gene            complement(14547..15047)
                     /gene="lspA"
                     /locus_tag="PLT63_04080"
     CDS             complement(14547..15047)
                     /gene="lspA"
                     /locus_tag="PLT63_04080"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOD97936.1"
                     /translation="MKKRNVIFLATVAGLLILDQVTKYVIHTGMALHESIPVIPGFFS
                     ITYIRNPGAAFGFLAGASPTFRYVFFIGITLAAIGLILHYLRVYADEDQLLTISLGMI
                     LSGALGNLIDRIRFGEVIDFLDFYIGANHWPAFNVADSAISGGAFVLFIYIIRMKKTA
                     SPSRTA"
     gene            complement(15164..17962)
                     /gene="ileS"
                     /locus_tag="PLT63_04085"
     CDS             complement(15164..17962)
                     /gene="ileS"
                     /locus_tag="PLT63_04085"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOD97937.1"
                     /translation="MDYKESLNLPKTDFPMKASLAKKEPEMLAKWDEMKIYHRIREVS
                     KGREIYILHDGPPYANGNIHLGTALNKIIKDMVIKTKNMSGLDSIYVPGWDCHGLPIE
                     HQVDKELGSKKYEISQADKRRACRAYAERFVDIQREQFKRLGVFGEWENPYLTMTYDY
                     EAITVAEFGKLFLNGDVYKGKKPVYWCATCKTALAEAEVEYGDHTTPSIYVKFPMISD
                     MAAVRPKLAGEKVNVVIWTTTPWTIPANLAIAFHEEFIYVAVKMEGEVLILAKDLLDY
                     CLDAFGWQGKPYEILDEFPGAVMEGLKTRHPLIDRESVLILAPFVTLDAGTGCVHIAP
                     GHGQEDYEIGMKYGLDNYAPVDEDGRFTKDVADFAGLFVFDANGPVNNKLKEVGALLG
                     LVDIDHSYPHCWRCKEPIIFRSTEQWFISMEKNALRKKALEAIAKGEWIPAWGKDRIY
                     GMVENRPDWCISRQRLWGVPITMFYCQECQSEFLTKEILDHVVALVREYGADVWFERE
                     VRELVPPGTVCPVCKKGREFRKETNILDVWFDSGVSHAAVLETRPDHRSPSDMYLEGS
                     DQHRGWFHSSLLECVGTRGRAPYKTVLTHGFVVDGEGKKMSKSVGNVVEPDEIITEYG
                     AEILRLWVAAEDYTDDIRISKEILTRLVEAYRRIRNTSRYILGNLFDFDPARDRLAYA
                     EMEEMDRWALHRLQEVIRRVRDAYDRYQFHMVYYTLYNFCTVDLSALYLDVLKDRLYT
                     SKADSRQRRSAQTAMEIILDAMARLLAPILTFTAEEVWNAMPTYKGKAESVHLTEFPA
                     VNPAFVDEELGEKWRTLVAVKGEISKAIEAARKSKTVGHSLDAAVAVAPPEKLRELIA
                     GHREDLQALQIVSQLDIVTRGDLDAGAYESKEIDGLYVGVTKARGQKCQRCWIYSEDL
                     GKDPEHPAICGRCLANLK"
     gene            complement(18137..19774)
                     /gene="groL"
                     /locus_tag="PLT63_04090"
     CDS             complement(18137..19774)
                     /gene="groL"
                     /locus_tag="PLT63_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019456581.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOD97938.1"
                     /translation="MAAKIIQYDEEARKSILKGVNTLADAVKVTLGPKGRNVILDKSF
                     GSPTVTKDGVTVAKEIELEDKFENMGAQMVREVASKTSDVAGDGTTTATILAQAIYRE
                     GAKTVAAGSNPMDVKRGIDKAVEAVVAELRKLSKPTKDQKEIAQVGTISANNDEGIGG
                     IIAEAMGKVGKEGVITVEENKGLETELDIVEGMQFDRGYLSPYFVTNAEKMEVSVEDA
                     FILINEKKISCMKDLLPILEQIAKMGKPLLIIAEDVEGEALATLVVNKIRGTLHVAAV
                     KAPGFGDRRKAMLEDIAILTGGKMISEDMGYKLENAKLEDLGRAKKIVIDKDNTTIID
                     GAGKRADLEGRVKQIRAQVEETTSDYDREKLQERLAKLVGGVAVIKVGAATETEMKEK
                     KARVEDALNATRAAVEEGIVPGGGVAYIRTLSALDKLKLENDQAVGANIVKKALEEPL
                     KMIAANAGMEGSVVVEKVKEKKGSFGFNARTDQYEDMIAAGVIDPTKVTRFALQNAAS
                     VASLMLTTQCMIAEKPEEKGAGMPPMPGGGGGYPGMM"
     gene            complement(19805..20098)
                     /gene="groES"
                     /locus_tag="PLT63_04095"
     CDS             complement(19805..20098)
                     /gene="groES"
                     /locus_tag="PLT63_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOD97939.1"
                     /translation="MKIRPLQDRVIVERLAEEEKTKGGIIIPDTAKEKPMEGKVIAAG
                     KGRVAEDGKLIKLDVKAGDKVLFSKYAGTEVKIDGKEYLIMREDDILGVIEGK"
     assembly_gap    20347..20446
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<20447..20695)
                     /locus_tag="PLT63_04100"
     CDS             complement(<20447..20695)
                     /locus_tag="PLT63_04100"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOD97940.1"
                     /translation="MQGKIFWLGHASIRIEADTVIYIDPWKVKGEPRADLILISHAHH
                     DHFSPGDIKKLRKKDTVIVGPADCTSQLKGDVRTMAPGD"
     gene            complement(20832..21833)
                     /locus_tag="PLT63_04105"
     CDS             complement(20832..21833)
                     /locus_tag="PLT63_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOD97941.1"
                     /translation="MIDSLGFVIFLVVIALAFDFINGFHDAANSISTVVSTRVLSPRF
                     AVMWAAFFNFAAIFIIGTPVAKTIGVGIIHPDIMDNYVIFAALGGAIIWNLTTWYLGL
                     PSSSSHALIGGLIGAGVLKAGIGTLVWGGIVKTALFIFLSPSIGMILGLGMMTLVLNL
                     SRNSNVTRSIKLFRKLQLCSSAINSLGHGMNDAQKTMGIIAIILYSKGLIGPVFHIPF
                     WVVFSCYTVIALGTISGGWRIVKTMGTKITKLQPIGGFCAETAAAISIIGASLGGIPV
                     STTHTIAGAIMGVGATKRLTAVRWGVAGTIVWAWVLTIPIAAMISAVVYSLSIIMIR"
     gene            complement(21882..22502)
                     /locus_tag="PLT63_04110"
     CDS             complement(21882..22502)
                     /locus_tag="PLT63_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HOD97942.1"
                     /translation="MRLIPREEKFYDLFEELADKIDEGAKLFLDMLHDYDSFATRLIK
                     LKELEHEADVITHRTYEKMHKTFLTPLDREDIYALVNKMDSILDMTEAAAIRMSLYKI
                     KYPSPELLEQAKLLNVATEKVKKIIQGLRDMKNAPMILEACVEINTIENAGDQILRTT
                     MAKLFETETDPIELIKWKEIFERFEETLDVCEDVSNIVEGIVLKNG"
     gene            22760..>23310
                     /locus_tag="PLT63_04115"
     CDS             22760..>23310
                     /locus_tag="PLT63_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOD97943.1"
                     /translation="MVWERKRILVVDDETDIVELIAYNLALEGYEIRKAGDGETALAI
                     IRAEKPALMILDLMLPGIKGLDVCRAVRRDRATEAIPIIMLTAKGEDTDKVVGLEMGA
                     DDYMTKPFSIRELTARVRAVLRRSESRPHREAEPAETAETFAAGDLFLDYAACKVAVA
                     GKKIELGPTEYRLLTFFTRHPGRV"
BASE COUNT         4563 a         6520 c         7332 g         4795 t
ORIGIN      
        1 cttccgccct cgctgccgga gagaaatccg ggatggcctc ttcccatccc gcggggagtt
       61 ctcccgccat gacccgccgg aattccgccg ccgcttcggg aaaggccgcc gcgtaagcgc
      121 ggaaggcttc ctcccaggct ttttgccggg ctgcgcctct ttccagggac ggacggcggc
      181 agtgggccaa gacttcctcg gggacgtaaa aagccggctc ctccggccag tccaggcgca
      241 ccttggcggc ggcgagttcc tccttcccca ggggcgagcc gtgacaggcg gcggtgcctt
      301 ccttcgtcgg ggcgccgtag ccgatgcaag tccgcacctt gatgagggtg ggccgccacc
      361 gttcctcctt ggcggcggcc agggccgcgt cgagagccgc gacgtcgttt ccgtccgcca
      421 cctccaggac ctgccagccc atcgcggcga aacgtccgcc cacatcctcg gtgaaggtga
      481 gggccgtcgc cccggcgagg gagacgccgt tgtcgtcata caggacgacg agttttccca
      541 ggccgagatg gccggccagg gcgcaggcct cggcggtgac gccttccatg atgtcgccgt
      601 cgctggccag gacataggtg aagtgatcaa cgatctcgtg tccgggacgg ttgtatgtcg
      661 ccgccagatg ggcctccgcc agcgccatgc ccacggcgtg gccgaccccc tggcccaggg
      721 ggccggtggt catctccgct cccggaggac ggccaagttc cggatgcccc ggcgtcttgc
      781 tttcccattg ccggaagctc cgaaggtcct cgatggtcag atcgtaaccc gtgaggtaca
      841 ggaggctgta aagcaacatg gaggcatgtc cggcggacag gatgacccgg tcccggtccg
      901 gccaggcggg atcggcggga ttatgcttga ggtggcgcat ccagagggtg taggctagga
      961 cggccgcccc catgggcatc cccggatgcc cggatttagc cttttcggcg gcgtcggcgg
     1021 ccagaaagcg aatggcgttg atgcatctct cttcgatcat tttactatac cttcttgatt
     1081 ttatagtgtt atgttgcttg ggaccggtgg gagaagattt catattttcg acggcgtgtc
     1141 aaccagtgaa acggccatgt tgaaaatgat tcaggcgttg gggccggcgc cggcggaatc
     1201 ctgctggaaa acgaggcccg tttatgataa tttcggccat catggtaaaa gggagggggg
     1261 ctatgtccgg agcgcttgaa tcgcttaagg tgctggattt tactacgttg ttgccgggac
     1321 cgttcgctac gatgtgcttg gccgacatgg gggcggacgt cctgcgcgtc acttcgggat
     1381 cccggcccga tctggtggaa ttcctgccac cccggctccc cgcgacgggg gtgggcgccg
     1441 tgagcgccta tctggggcgg ggaaagcgtt ccctggccct gaacctcaaa ggacccccgg
     1501 gggatcgaga tcgtccatcg gctgctggcg gactatgaca tcgtcatcga acagttccgg
     1561 ccggggatca tgaccaagct gggactggat tacgagcgcc tgaaaaaggt caatccccgg
     1621 gtgatctact gctccatctc cggatacggc cagatgggtc ccctgcggga ccgggcgggg
     1681 cacgacatga actatatggc cctgtcgggg gcgatgggct actcggggcg gaaggcgacc
     1741 gggccgaccc tcatgggaat ccagattgcc gacatcgcct cgggttccaa caatgccgtt
     1801 atcgggatcc tggcggcggt ggtgagccgc gccgcgaccg gggaggggca gtatatcgac
     1861 atctccatga ccgacggggt gatggccctc aacgccatgg cgggggccgc gtatcttgcc
     1921 gggggcgagg cgccgggacg ggaggaaaac tggctcaacg gggggtcgct ctacgatttc
     1981 tacgcgacga aagatggcgg ctatctgagc atcggcagcc tggagccgca gttcttccgg
     2041 accctctgcg aggtgatcgg ccgtcccgat ctcatccccg gcggtgtaca gcccaaagct
     2101 ggggatttcg agcgggttcg ggaggagatc cgggcgatcg tccggacgaa gacccgggcg
     2161 gaatgggagg cgattttcaa ggaggtggac gcctgcgtgg agcccgtcct gtccctggcg
     2221 gaggccctgg agggcgaact ggcccggggg cgggagatgg tggtggacgt gccgctggcg
     2281 gcgcccgacg gctcggacgt gggtagggga gacgcggggg gtggcggtgg cgcactggga
     2341 gcgggtgatg gcgcctttgc ggcgggtgtt cagggtgcag gcaaagcttc gacgataaag
     2401 gaaagcggcg cccgcggcac cgccggggcg gcggggggtg ggggtgcatc cgggaaagcc
     2461 gcgggagacc ccggggccgc cactggggga atcgccgcgc cacgcgccac ggtgcgccaa
     2521 ctggccaatc cggtgaagtt ctccagaact ccggcctcct acgcctttgc gggtgtcaag
     2581 acggggactc acaaccggga ggtcctcaag gccctgggtt atacggaggc gcagattgcc
     2641 gaattcgccg ccacgggtct cttcgattaa aaaaagacta agaaaaaaag gaggaaatga
     2701 cgatggcaac gtatgagtat cgctgcgagg catgcaaaaa gtcttttacc ttgatccaga
     2761 agatgtcgga gcatggcaag gcgaaggtga cctgccccaa atgcaaaagt gagaaggtca
     2821 aacagcagat cagcggtttc ctgatcaaaa ccagccgtaa aagctaaaaa aaatgaaaaa
     2881 aagagggaag gctctttttt ccccgtcccc cttttttccc gattatgcct tgacactagg
     2941 accgatcttg attatagctc caccatcaat ctgtctatat agataatatg agtcctttgc
     3001 gtagcggcag aaatcagaaa cgcgctgcct gcttcgtgcc gggatgacca aaaagagagg
     3061 gaatcagcag catgggagat ccgcccgcac ggacgccgga agatgccggc gccggttatc
     3121 gaagggacca gcaaccgagt cagcccggcc caccgccgtg ccaaacagaa aatgctaatt
     3181 ttaaagacct gaccccatat gatgaagatg agatcaacct gctggatctg gggcgggtga
     3241 tctggaaacg gcggcggctt atcggttata tcgcggcggc gatggtcttc ctgacggtgg
     3301 tggtctccct gctcatgacc aacatttatc agtccgaggc ggtgatcatg ccggttgccg
     3361 gcaaggaggt gggcgcgggt ggcggagcgg cggcgctgct ggccgcccag tttggcgggt
     3421 tggccgggat tgcgacgccg ggctccacat cgacccagga gatcgtcagc cttctgaaat
     3481 ccaatgtgtt gcgggagaag gttatcgagc agcacaaact gctgccggtg cttttcgaag
     3541 aagaatggga tgcggagaag aagcgctgga aggcggaagg gggcggattc cgtataaacc
     3601 tgaatcccat ttcctgggtc tccagccttc ttgccttggt gcggccgcct cccccgccca
     3661 gcatcgccaa aaagaagccg ggaattcccg acgtctggga cggcattcgg gccctggatg
     3721 agatggtgac ggtgaagcac gacgtgaaac agggctccat taccattacc ggtcaattcc
     3781 ctgatcccga actggcggcg agggtcgtgg agaatttcct gactacgctc acggaccaca
     3841 tgagcgccga ggcccggcgg gtggccctca ccaaccagaa atatctggag ggtcagttaa
     3901 gcgcgacgac agaccccttc atcaagcaaa agatctacaa cctcatcgcc cagcaggtgg
     3961 agacctccat gatggcggag gtgaaagaga atttcgcctt caagatcctg gatcctccca
     4021 gggtcccgga ccagaagttc aagccgaagc gggcccagat ggtcatgttg tcctttgtcg
     4081 tggcgctctt catcgggatc ttcatggcgt ttttccttga gtttctggaa aagcaaaaca
     4141 tacacataga cgccggtcgc tggccgctga tcaatcgcct tgccggctgg aagcggaggt
     4201 tgctgatttg aatattagac gtttcttcgg catcggaatt tttatcctgg cgattgcatc
     4261 cataatcggc gctctgcccg ctgtcgccca gaccccgaat attaccgcgc agcccctgcc
     4321 ggggatgatg ggacagcaga tgcccactac agtcaccgcc gatgtctgca agaaccttcc
     4381 ggcaagccag cgggctgttt gcgaggcgga gatccagcgg agcggcggcc ggttgacccc
     4441 ggaggccctt caggccatca aggagcggcc ggcggtaacg ggaacgacac ccgatgaagc
     4501 cgcacgggtc cggcagctca cggaacagaa ggaacgccaa aagataaccg ttgaggaata
     4561 caaggaaaaa acaatattcg accgcgctcg gaatatcgga aagtatcagg atgtatccac
     4621 ggacctgaag cgcttcggcg ccgatttttt cagcgaggcg gaggtgagcg ttaccgttga
     4681 ccgcaaggac gtgcccgtgc cgatgagtta tgtcatcggc cccggcgatg agatcaagct
     4741 tctcctctgg ggtcgcgtca acgcccagta cgacctcata gtggaccggg acggcaagat
     4801 caccatccct cagatcggac ctctccatgt gtccggcctg accttcgagc agatgtccaa
     4861 actggtcatc aaacaggcgg aacagattgt gggggccaat atcgacatct ctatgggatc
     4921 gcccaagatg atccccatct tcgtgatcgg cgacgtcaag cgtcccggtt cctacaccgt
     4981 cggttccttc tccacgatca ccgacgccct gctcacggcg ggggggccca cggaaatagg
     5041 ttccatgcgc aacgtccagt tgaaacgtaa aggccgcctc gtgacgacct tcgaccttta
     5101 cgatctcctc ctcaaaggcg acaagagcca tgacgtagcc cttttggccg gcgatgtgat
     5161 cttcgtcccc atcaccgggc cggtagccgg catcgccggc aacgtccgcc gccccgccct
     5221 ttacgagttg aagcaccgct acgatttgga atatctcctg gaactggccg gcggcatcac
     5281 cccctccgcc tacacccagc agatccagat cgaacggatc gtcaggaacc agcgccacat
     5341 cgttatggac atcgacgaca agaagatggc caaggctgcg gagatcgctc tccacgacgc
     5401 cgacctggtg aagatctttc cgatcatgga tatcaacgtg aacgtcgtct atctcaacgg
     5461 caacgtcaag cgtccgggga aatacgagat gaaaccgggg atgcgcgtgg gcgatctgct
     5521 caagaacagc gatgacctgc tgagtgaaac ctacctcgaa tacgccctca tcaagcgtct
     5581 ggagccgccg gacatgaaaa ccgtcctggt gcccttgaat ctggagaacc tgctgctgaa
     5641 aaaggatgaa cgggcgaatg tggagctgaa gccccgggac agcatctatg tcttccatca
     5701 gggcctcttc aaggacagcc cctttgtccg gatcgaaggg gagattcggg gtgactgcgc
     5761 gacagataag gaagcagaaa caaccggtga gatgacaaga gagtccctgg ctggaatgag
     5821 ggcgatgaca agcgagcccg ggggtggaat gaggactggg atgactgtcg atacccgaac
     5881 ggctgccggc ggcaccgcgg cggcgggagc gggatcggaa gaatcccgct acagtcagta
     5941 cttgctttcc cagagccggg cggaaaaacc tcgctacagc caataccggc tttcccagag
     6001 cctgaaggca atcgaagagg atctgaagaa acagggattg actgacctgg cgaagcaggt
     6061 ttggactatc gagagcgagg tggccgacaa cctgaaaatc cctccggaag atttccgcaa
     6121 tttgcgtacc cagttggtca aggtgggccg ccccgatctc gctaaccagc ttctggaaat
     6181 cgaacagaag gtgaaaaaag gtggcgccct ggctgatgtc accgcggaac tggtcctgat
     6241 cgagaacatc ctcaagcgca tgaatatgta cgatcaggcg gcaacggtga ggagcatcca
     6301 tgacgagatc aaaaacttcg gccgggtccc cacggaaaac atgcgtgttc tccgggcgga
     6361 ccttatccgc accaatcagg ctgatctggc caaccgcttg ttggagatcg agaatcggac
     6421 caagctcagt tgcaacatcc cctttgtgga aaatatgcgc gtcaaggacg tgatcctcat
     6481 ctccggagga ctgaccagca actcctatct ggaccgggcg gagatcatcc ggacgaacaa
     6541 taaacggcag taccagaccc ttttcttcga tctgggcaag gcgatggccg gcgaccccca
     6601 gcataatctt ccgctccaga acgaggatca gatcgtcgtc cattccatct gggagcaggt
     6661 ttacaagaag catgtggcga tcgacgggga tgtcgcccgg cccggaacgt atcaatatac
     6721 cgaggggatg accgtccgcg acctcgtttt caagggtggc aaccttctgg aatcggccta
     6781 tctgggagag gcggagatct catccatcgt cgtggtggcg ggaaaatcat ccgcgacggg
     6841 gcggcgggtg atcaatctgg gccgtgccct ggcgggagat ccccagcaga acgtggtttt
     6901 gatgcccaac gaccggcttc tggtcaagcg catccccaac tggggctccc tgcaatacgt
     6961 aaaactgtcg ggggaattca agttccccgg gaaatactcc ttccacaaag gggaaaagct
     7021 ttcgtcggtg atcgagcggg ccgggggtta tacggacaac gcctatctcc ggggggccgt
     7081 cttcaaacgg gagaccgtcc gggagttgca gcagaagagc cttgacgaaa tggcccgccg
     7141 catggagcgg gaactgcttt cggagagctc cgcccgggtg gcgacgtcca tcagcgccga
     7201 ggagatggcg gccaagaagc aggaagcgga gctgaagctg aaactcatcg agtacatgaa
     7261 atccctccag gccacgggaa ggatgaccat cgccctgagc cacgtccgga tgctcaaggg
     7321 cggtgctttc gacatcgaac tcgagagcgg cgacaacctc ttccttcccc agaagacgag
     7381 cgtggtcaac gtggtcgggg cggtgatgtc ggaagggtcc cacatctata acgacagcct
     7441 ggattacaag gactacgtcg ccctggccgg gggttatgcc cggtatgccg acgaggccaa
     7501 tgtatttgtc atcaaggtag acgggagcgc ccggaagctc tcctcgggct tcgctacctg
     7561 gaacgacaag cgggatcgct gggagatgac ggcctatggc aaggaaatca aacagatcga
     7621 accgggggat gtcatcgtcg ttccggagcg gatcgcccag atcgcctggc ttagggagat
     7681 caaggacatc actcaggttc tcatgaacac cgccgtcacg gcggcaacga ttatcaaact
     7741 ctggtaaagg aaaaaagtcc ttgacataat agtaattaaa ataatataag agaacaaaaa
     7801 aagatgtaga gttatatatt aataatatca atgagataag gaggaaagag aggaagagta
     7861 tgaaaacgat tggcaagtac acactgattg tcgggatgat cttttttatg gcgatgctgc
     7921 ccgtgagcgt ctttgcccag gctcaaggcg cgggtgccgg aacggctgga gcggccggcg
     7981 cgggtgccgg aacagctgga gcggccggcg cgggtgccgc ggccggaggc ggggctttgg
     8041 ccggtatcgg ggcgggaacc atcgctgcag ccgccgtggc ggcagcggca atcgccacag
     8101 cagcgatcgc ttcctcttca tccaagagcg attcgacgcc cgcggcccat tcggcgacgt
     8161 cacaccacta agcagtgaag ttggctactc ttcacggata tgacatcgcg tcaagagtag
     8221 cttttttatt tccatcctta tttgcatagc acgccgggaa gctcacggcg tgctattgct
     8281 ttcctgaatt catgaagcga agaacgatct ttttgcggtg ggcggtgatt gccgttttga
     8341 ttgccgcatc ccagcttatg gcaccttgtg ctgcggtgac gcagtccctg agcccggagg
     8401 cggaaacggt gctgaatgcc gccgaggggg tcttcaaggc catgaaggcg agagactatc
     8461 cggcgatatg ggcgggactg accgccaaga ccagaaacga gatggtggag agtgtccgca
     8521 aaggcgccgg aaaaaccggg cacgagttta cggcggcgca gctaagcaat gacttcgcca
     8581 ccggcgggcc ccaggctaag gcttactggg attcctatct cgacgtcttc aatcccgacc
     8641 ttgtcctgga acagagccgc tggtccctgg gatcgatcaa aggcgatggc gccacggtca
     8701 tcatccagta ccgaaaatcg gaaaatccgg cgatcctcat cctgaagaag gaagacggcg
     8761 cctggcgcct cggactggaa gaaacctttg gcccccgccg ctggatcatg aaataatagg
     8821 ggaagtagcc ccctattata atgtcgaaaa aatccatcga taagaaaccg cggactgcct
     8881 ttttctgcca gagctgcggt catgattccc cgaaatggtt ggggagatgt ccgggctgcg
     8941 gggagtggaa caccttcgtc gaggaggagt tgcgggagac ggcggcaggg ggagcagggg
     9001 cgtccgggcg ggcgtctttt tcattttcgg gggccccgca acccatcgag gctatcgagg
     9061 cggacgaggg ggaacgcatc cggacgggca tggcggagat ggaccgggtt ctggggggcg
     9121 gcatcgtccc gggatcggcg atcctggtgg gcggcgatcc ggggatcggc aaatccaccc
     9181 tgctcctgca actcctccag caactggcgg cgcaggggat gaaggtcctc tacatcaccg
     9241 gcgaggaatc ggccccgcag atcaagctcc gggggcgacg gatcggggcg gaggcggcaa
     9301 atctcttcgt gctggtggag atcgacctgg acagcatgat gcggcaaatc gaggccacca
     9361 ggccccaggc ggtggttgtc gattccatcc agacggtctg ctccgccgcc ctgggttcgg
     9421 ccccgggcag tgtcggacag gtgcgggaat cgtcggagcg cctgatcatc caggccaaga
     9481 agacgggtat ccccgtcttt ctcgtcggcc atgtgacgaa ggacggcgcc attgccggac
     9541 cgaaggtctt ggagcacatg gtggataccg tcctgtactt cgagggggac agcggacatg
     9601 ccttccggat catccggggg ataaagaacc gtttcgggcc tacgaacgag atcggcgtct
     9661 tcgaaatgcg gggcgacggc ctccgcgagg tggccaatcc ctcggcgttc tttctggcgg
     9721 aacggccgga gcgggcggcg ggctccgtgg tggttcccag catggagggg acgcggccca
     9781 tcctcatcga gattcagtcc ctcgtgatcc cgacgaattt cgggatgccc cggcggacgg
     9841 ccatcggcgt ggacgccaac cgcgtgtcgc tcctggtggc ggtcctggac aaggtctgcg
     9901 gcctccatct gagcaatcac gacatcttca tcaacgtcgc cggaggcgtg aaggtgagcg
     9961 aaccggccat cgatctgggg attgtctcct ccctggcatc gagctttctg gaccgggcgg
    10021 tggtgccggg gaccgtggtc tgcggggagg tgggcctggc cggggagatc cgggggatca
    10081 gccgcgccga tgtccgcatc cgcgaggcgg cgcgtctggg tttttcccgg tgcatcctgc
    10141 cctttgttgc cccccgggga gaggcgggga cggacgccgc cgcagcggcc aaacctgtcg
    10201 catcatccgc cacacccgcc aaatccacct cactatcggc gttgcggtcc gccgtgccgg
    10261 cggctagagc cgccattgcc tcggcttccg accgaggcgt cgcttcgcaa aggggggggg
    10321 ggcaggaaag cgacattgcc ctccttcccg tccgctccct cccggaactc cttagccatt
    10381 tgttctgata tttattgttt acacctttcc ctccccttca aggggagggt tagagtgggg
    10441 atgggattca tctcagtttg aagagcttct cgatcaactg cgatttgaga agatcgccgc
    10501 cgatcttgat ctggcccgag gtgtaggcct gcatcgccga aagcttgccg cccatcatgt
    10561 cgaggaaatc cccggcggcc atggtgatgg tcgtcgtcgg ggcgtcccgc ttgcccgcct
    10621 cgatccggca ggccccgtcc ttcacctccg cgaaccagtc gccgccgccg tcgccggtga
    10681 tgtgaaactg gaagacgacg ttgaccccgg ccgccttctc ggcctggaag gcctgctgca
    10741 tctgggcgaa gatccccgcc acggtgggcg gagcgggctg ggcgggggtg ccgtctcccg
    10801 cggttgcagc ggctgcgatg gccgaggcgc cgcctgacga tccggggtcg gaggaggttg
    10861 tctcggcggc ggccgctttg ggcttcgggg cgaaggcatt gaggagatcc gtcacctggt
    10921 ccatgagctg gaaatactcc ttggcgcctt ccatatcgcc gatcttctcc cagttgtccg
    10981 ccacttcctc taaggtagga atccgccggc cgtctccgat gaccgtccct gggccggtga
    11041 ggatggcggc gcggttgtag aaacccatcc cggcgttgaa gatgtgtccc gttaccccgc
    11101 acgtctcgga gacgaggtag aggacaatag gggcgacgaa ttcgggtttg agtttggcga
    11161 agatgtcggg gggaaggatg tcctcggtga gacgggaggc ggcgatgggg gcgatgctgt
    11221 tgacctttat gttgtacttc tgtccttcga tcttgatggt gttcatgaaa cccacgaggc
    11281 ccagcttggc ggcggagtag ttggtctggc cgaagttgcc gtaaagtccc gaggcggagg
    11341 tggtcatgac gatgcgcccg tagccctggt cgcgcatggc ggcgaaggcg ggcctggtga
    11401 cgtggtaggc gccgttgagg tgaacgtcga tgacggcgcg ccagttttcc gtctccatct
    11461 tgaggagccc cttatcgcgg ataatgccgg cgttgttgat gaggatgtcg aggcgtccga
    11521 aggagtccag ggcggttttg acgattctct ccccgccctc gggagtggcg acgttgtcgt
    11581 agttggccac ggcctcgccg cccgcggcct tgatttcggc tacgaccttg tcggcggggg
    11641 aactggagcc gtccccggcg ccgtcgcggg cggcgccgaa gtcgttgatg acgaccttgg
    11701 cgccccgttt ggcgagctcc agggcataca cccgccccag gccgcccccc gctcccgtga
    11761 tgaccgccac ccggccgtca aaacggatct gagccccggc ggcgggtccg tactcgaaga
    11821 tgccgttgtc aatgacgaca tcgccggttt tggctttcag ggtacgccat acggcccgtc
    11881 cttccccggt cttccagatg agggtccgga tgggctcgcc ggggtaaagg ggcttcgaga
    11941 aacggcaggc caagcgccgg accagatccg gctgccccgg ggtcagtttt tccatgaggg
    12001 cgcgacaggc aaaaccgtgg gtgcacaggc cgtgcatgat gggcttttca aagcccacct
    12061 gccgggcaaa gtcgggatcg acgtggagct gaaagaaatc gccggagagg cggtagagga
    12121 gcggctgatc aggggagggt tgggccgtga ccacgaagtc gggttcggac gccgggaata
    12181 cgaagggttc ggtcttccct tcctcgccgc cgaagttgcc gtcgaagcgg ctgaacatgg
    12241 taatgacatt tttgaagagc ttattgccgt cggcgtcgca ggtgtctcct gccgcgatga
    12301 cgacggcgcc cttatccttc ttgtcgtagt agtgggtgat tgacccctcg gtcgtcaggg
    12361 ctcctccggt ggggatgggg cggtagaaga ttatgtcctg ctcgccgtga agaatgcccg
    12421 ccaggttcac gttggcggct ctggccacgt gccggaagaa gccaaagata ccggccacgg
    12481 aaaagctggg gatgaccttg aggttttttt cgtaaacgta atccagttcg tcgaagcccg
    12541 cgccgattcc cagagcgtac agggcggcgt ccttccaggt gtaggtcttg gtgaccgggc
    12601 cgattttctt gccgattgcc tccaaattga gcgccataag tgtaacctcc ttatctgttc
    12661 ggagcggcct ttgatttatt gttgcgcggc gcgccttttg tcatccccgc cccggcgaaa
    12721 aaggtgtgtt ttatgagctg tccacaattc atcattcttc cggatgccga cgcaagcacg
    12781 gtatgacaag cggggccgct ttcaaaggtc gctcggattg atttggagta caatggaaat
    12841 catgaccttt tgtcaagaac aattatcgat ggaatatttt cacaatcgga agacgacata
    12901 tggcttgcgc cgcggtgtga atcgtgaata ttgatgaact cgcaaaaagt catcattgag
    12961 acgacgaagt aaaaagctcc agaggcaagg cgcgcgaatc ctgaggaatg aggcgtactt
    13021 gtgtgtacgt cgcagtgacg aaggatgaag cgcaacgccg catatggact ttttacgatg
    13081 tcgtcaacat tgaagacttc atcctgcgct tgtggattag tgcttgcccg gaattcctga
    13141 gtcgcatata taaaacatgc ccatatacag gcaggatgcc gtgatggtgg gtgcagattc
    13201 ggtacggacg tggcgtgtgt tcgccggctg gtctgatgac gaagaggcgg agcctcggga
    13261 aggcggcggt cgcctgatcc ggcgggcgtc acgacacgac ccgggacagg ggcggggacg
    13321 ggaatgaaat ccggcgatgc cgcggccgga taagatgcgc cggattaaga taaggaggga
    13381 gagcatgttt caagatgttg cccgggagtg gatcgacgag ttgaccgcgg cgggacggat
    13441 ggaaaaccac gacgaggagg gccggcgcgc cctgcgccgg gattacgcgc agcgaataga
    13501 agagtttttt atcgccgcgg tgacaaaaca gttggaaccc atgggaaagg tgtccgattt
    13561 cgaacggatg ctcgtctggg acacccagta cacccacaag tttctcaatc agacgattcc
    13621 cggctatccg tccttcaaga gggagattct cgcgaaggcc cggaaggcga tcctcggcga
    13681 cgctacgcct gccgaatcag caacgaaatc ctgaggggcc gccgcgggtg aagatcatct
    13741 ttctcggcac caacgggtgg tacgatacgg atacgggcaa tacgatctgc accctcttgc
    13801 ggacggagga ttatgacatc ctcctcgatg cgggcaacgg cctggccaag gcggaccaat
    13861 acggcaccgg ggccgacggc cggcccgtct atctcttcct cagtcacttt cacctggatc
    13921 acgtggcggg gcttcacacc ctgacgaaat tctccttttc cggcggactt accatctgcg
    13981 gtcccgccgg ttcccgggac atcctgacga cgctggtgaa tcagccgttc accgtgccct
    14041 tgaaggatct tccctacccc gccgccgttc gggaactgcc aggggaagtg aagtcgcttc
    14101 ccttcccggt tacggccctt ccgttacgtc atgcctcctt gacgctgggc ttccgattga
    14161 ttctggacgg cctgacggtg agctactgcc ccgatacggg ctactgtgcc aacgccgtaa
    14221 ccctggcccg ggaggcggat cttttgattg ccgaatgcgc ctaccgccgg gggcagggca
    14281 gcgaggactg gccccacttg aacccggaga cggcggcgcg gatcgcgggg gaggcccggg
    14341 caaagcgtct ggcactggtc catttcgatg cccggatcta tcccaatctg gagctgcgcc
    14401 gggaggccga gcgggatgcc cgcgccatct tcctctccag cttcgccacc ttcgatgacc
    14461 tggaaatcga actgagcccg tcacttttgt gatcgtttaa actgacggaa caggagaaat
    14521 aggggaagcg tccccatatt tcatatttat gcggttcggg agggagacgc tgtctttttc
    14581 atccggatga tgtagatgaa gaggacgaag gcgccgccgg agatggcgga atcggccacg
    14641 ttgaaagcgg gccaatggtt ggcaccgata tagaagtcga ggaagtcgat cacctcgccg
    14701 aagcggatcc ggtcgatgag gtttcccaag gcgccggaaa ggatcatccc cagggagatt
    14761 gtaagcagtt gatcttcgtc ggcatagacg cgcaggtaat ggaggatgag gccgatggcg
    14821 gccaaggtga tgccgatgaa gaagacgtag cggaaggtcg gcgaggcccc ggcaaggaag
    14881 ccgaaggccg ccccggggtt ccggatgtag gtgatgctga agaatcccgg gatcacggga
    14941 atcgactcgt ggagggccat gcccgtatgg atgacgtact tggtgacctg atcgaggatc
    15001 agcagccccg ccacagtcgc caaaaagata acatttcttt ttttcatgaa taagtctgcc
    15061 gtcagatgga tcaagttgcc tctaaatctt ttccctcccc cttcaagggg gaggttaggt
    15121 gggggatggg gtacgacaaa ttcatccctc cagcgctgcg tctttatttg aggttcgcca
    15181 ggcagcggcc gcagatggcg gggtgttccg ggtccttccc caggtcttcg ctgtagatcc
    15241 agcagcgctg gcacttctgc ccccgggcct tggtgacccc cacgtacagg ccgtcgatct
    15301 ccttgctttc ataggcaccg gcgtccaggt cacctcgggt gacgatgtcc agttgcgata
    15361 cgatctggag ggcctggaga tcctcccggt gtccggcgat aagctcccgc agcttctccg
    15421 gcggggcgac ggccaccgcc gcgtcgaggg aatgacccac agttttactc ttccgggccg
    15481 cctcgatggc cttggagatc tcccccttga ccgcgacgag agtccgccac ttctctccca
    15541 gctcctcatc cacaaaggcc ggatttacag cggggaactc ggtgagatgg acgctttccg
    15601 ccttcccctt gtaagtgggc atggcattcc agacctcttc ggcggtaaag gtgaggatcg
    15661 gggccagcag ccgggccatg gcgtccagga tgatctccat ggccgtctgg gcggagcggc
    15721 gctgccggga gtcggccttt gaggtgtaga ggcggtcctt gaggacgtcg aggtacaggg
    15781 cgctcaaatc cacggtgcag aagttgtaga gggtgtaata gaccatgtgg aactggtagc
    15841 ggtcgtaggc gtcccggacc cggcggatca cctcctggag gcggtggagt gcccagcggt
    15901 ccatttcttc catctcggcg taggccaggc ggtcccgggc cgggtcgaaa tcgaagagat
    15961 ttcccaggat gtagcggctc gtgttgcgga tccgccggta ggcctccacc aggcgggtta
    16021 ggatctcctt ggagatgcgg atgtcgtcgg tgtagtcctc cgccgccacc cagaggcgca
    16081 ggatttccgc gccgtattcg gtgatgatct cgtccggctc gacgacgttg cccacggatt
    16141 tggacatctt tttgccctcg ccgtccacga cgaagccgtg ggtcaggacg gtcttgtaag
    16201 gcgcccggcc ccgggtgccc acgcattcca ggagcgacga atggaaccat ccccggtgct
    16261 ggtcgctccc ttcgaggtac atgtccgacg gggaacggtg atcgggacgg gtctccagaa
    16321 cggcggcgtg gctcacgccg gagtcgaacc agacgtcgag gatgttcgtc tccttgcgga
    16381 attcccggcc ttttttgcag accggacaga cggtgccggg gggtacgagc tcccgaacct
    16441 cccgctcgaa ccagacgtcg gcaccgtatt cccgcaccag cgccacgaca tggtcgagga
    16501 tctctttggt gaggaattcg ctctggcatt cctggcagta gaacatggtg atggggacgc
    16561 cccagaggcg ctggcgggag atgcaccagt ccgggcggtt ttccaccatg ccgtagatcc
    16621 ggtccttgcc ccaggcgggg atccactcgc ctttggcgat ggcttccagg gcctttttcc
    16681 ggagggcgtt cttttccatg gagatgaacc attgctccgt ggagcggaag atgatgggct
    16741 ccttgcagcg ccagcagtgg ggataggaat ggtcgatgtc cacgaggccc agcagcgcgc
    16801 ccacttcctt gagcttgtta ttgacggggc cgttggcatc gaagacgaac aggccggcga
    16861 aatccgccac gtctttcgtg aagcgcccgt cttcatcgac gggggcgtaa ttgtccaggc
    16921 cgtatttcat gccgatttca tagtcctcct ggccgtggcc gggggcgatg tggacgcagc
    16981 cggtgccggc gtcgagggtg acgaaagggg cgaggatgag gacgctttcc cggtcgatta
    17041 gggggtggcg ggtcttgagg ccctccatga cagccccggg gaactcgtcg aggatctcgt
    17101 agggcttgcc ctgccagccg aaggcgtcga ggcagtaatc caggaggtct ttggccagga
    17161 tcaggacctc gccctccatc ttcacggcca catagatgaa ctcctcgtgg aaggcgatgg
    17221 ccaggttggc ggggatcgtc cagggggtgg tcgtccagat gaccacgttc accttttccc
    17281 ccgccagttt gggacgcacc gcggccatgt cggagatcat ggggaacttg acgtagatgg
    17341 acggcgtggt gtggtcgccg tactccacct ccgcctccgc cagggcggtc ttgcaggtgg
    17401 cgcaccagta aacgggtttc ttgcccttat acacgtcgcc gttcaggaag agcttgccga
    17461 attcggccac ggtgatcgcc tcgtaatcgt aggtcatggt caggtaaggg ttttcccact
    17521 ccccgaagac cccgaggcgc ttgaactgtt cccgctggat atcgacgaag cgctccgcgt
    17581 aggcccggca ggcgcggcgc ttgtcggcct gggagatttc gtatttcttg cttcccagtt
    17641 ccttatctac ctggtgttcg atggggaggc cgtggcaatc ccagccgggg acgtagatgc
    17701 tgtccagacc cgacatgttc ttcgtcttga tcaccatgtc cttgatgatc ttgttcaggg
    17761 ccgtgccgag gtggatattg ccgttggcgt aaggagggcc gtcatggagg atatagatct
    17821 ccctgccttt ggaaacctcg cggatccggt gatagatctt catctcatcc catttggcga
    17881 gcatttccgg ctctttttta gccaggctgg ctttcatggg gaaatccgtc ttgggcagat
    17941 tcaagctctc tttgtagtcc atggcacgct ccttccttca tcgaaatcgt aaggcgggaa
    18001 actaccacag aaaaaggatg ctttcaagga gaatgcgggg caatagcaaa gccccctgca
    18061 acaaaagcac agggggcttt gcttgtatta gctgagataa cttatttccg tcgtaccgtt
    18121 accaaagcgc tttttgttac atcatgccgg ggtagccgcc gcctcctccg ggcatggggg
    18181 gcatgccggc gcccttttct tcgggcttct cggcgatcat gcactgggtg gtcagcatca
    18241 gggacgctac ggacgcggca ttctgcaggg cgaagcgggt gaccttcgtg ggatcgatga
    18301 cgccggcggc gatcatgtct tcatactgat ccgtccgggc gttgaagccg aaggaaccct
    18361 tcttctcctt taccttctcg acgaccacgg agccttccat gccggcattg gcggcgatca
    18421 tcttcagggg ctcctcgagg gccttcttta cgatgttggc gccgacggcc tgatcgtttt
    18481 ccagtttcag cttgtccagg gcggagaggg tgcggatgta ggccacaccg ccaccgggga
    18541 cgatgccttc ttccacggcg gcgcgggtgg cgttcagggc gtcctcgacg cgggccttct
    18601 tttccttcat ttccgtttcc gtcgccgcgc cgactttaat tacggccacg ccgccgacga
    18661 gtttcgccag ccgttcctga agtttctcgc gatcgtaatc ggaggtggtc tcctcgacct
    18721 gggcgcggat ctgttttacc cggccttcga ggtcggcccg ttttccggcg ccgtcgataa
    18781 tcgtcgtgtt gtccttgtcg atgacgatct tcttggcccg gccgagatcc tcgagtttcg
    18841 cattttccag tttgtatccc atgtcctcgg agatcatctt gccgcccgtg aggatggcga
    18901 tgtcttccag catggcctta cggcggtcac cgaagcccgg ggccttgacg gcggccacat
    18961 ggagggtgcc gcggatcttg ttgacgacca gagtcgccag ggcttcgcct tccacgtcct
    19021 cggcgatgat gaggaggggc ttgcccatct tggcgatctg ctcgaggatg gggaggaggt
    19081 ccttcatgca actgatcttt ttctcattga tgagaatgaa ggcgtcttcc acactgactt
    19141 ccatcttctc ggcgttggtg acgaaatagg gggagaggta gccgcggtcg aactgcatgc
    19201 cttccacgat gtccagttcc gtttccaaac ccttgttttc ttcgacggtg atgacgcctt
    19261 ccttgccgac cttgcccata gcctcggcga tgatgccgcc gatgccttcg tcgttattgg
    19321 cggagatggt gccgacctgg gcgatctcct tctggtcctt cgtgggcttg ctgagcttgc
    19381 ggagttcggc gactacggcc tccacagcct tgtcgatgcc gcgcttcacg tccatggggt
    19441 tgctccccgc ggccacggtc ttggcgcctt cgcggtaaat ggcctgggcc aggatcgtag
    19501 cggtggtcgt accgtcgccg gcgacgtcgc tggtcttgct ggcgacttcc cggaccatct
    19561 gggcgcccat gttttcaaac ttgtcttcca gttcgatctc tttcgctacg gtgacgccgt
    19621 ccttggtgac ggtgggggaa ccgaaggact tgtcgagaat tacgtttctg cctttcgggc
    19681 cgagggtgac cttgacggcg tcggccaggg tgtttacgcc cttgaggatc gactttctcg
    19741 cttcttcatc atactgaatt atttttgctg ccatatgtat ttaatcctcc ttagtcaggt
    19801 ttttttactt gccttcgatg acgcccagga tatcgtcttc gcgcataatg aggtattcct
    19861 tgccgtcgat cttcacttcc gtcccggcat acttgctgaa aaggaccttg tcgcccgctt
    19921 tgacgtcgag tttaatgagt ttgccgtctt cggcgactct accctttccc gcggcaatga
    19981 ccttaccctc catgggcttt tccttggccg tatcggggat gatgatgccc cccttggtct
    20041 tttcctcttc cgccaagcgc tccacgatga ctctgtcctg caatggcctg atcttcatgc
    20101 tttcctctcc tttcaccaaa tatatttaag atttttagtg gctgtttata tcctgggtat
    20161 gtcgcggcat aacatataca tcaaatgttc cgtgtcaagt tcaaggtcgt atttttttta
    20221 tcgttaatat ggggacactt cccatatttt tcccagagcc ggaaaaaaag gagaaccgtc
    20281 cccatttccc cattttcgtc ttaaggcgcc gctatggcaa tttggtataa ggtgcctctc
    20341 ttcctcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnatcg cccggcgcca
    20461 tggtccggac gtcgcccttc agttgggagg tgcagtccgc ggggccgacg atgaccgtgt
    20521 ccttcttgcg cagttttttg atgtctcccg gggagaaatg atcgtggtgg gcgtggctga
    20581 tcaggatcag atccgcccgg ggttccccct tgaccttcca ggggtcgata taaatgaccg
    20641 tatccgcctc gatgcggatg ctggcgtggc ccagccagaa gatcttgccc tgcatgttac
    20701 acctccgaga atggcccttc gaacgctaaa ccaattaccg taaatattcc aatacttcaa
    20761 gtttattttt attaatacgt atggttgtcg ttactcggga agtggataaa accggtgcat
    20821 gaggacaggc gctacctgat catgatgatg ctcaggctgt aaaccacggc ggagatcatc
    20881 gcggcgatgg ggatggtgag gacccaggcc cagacgatcg ttcccgccac tccccaacgt
    20941 accgccgtca gccttttcgt ggcccccacc cccatgatgg ccccggcgat ggtatgggtg
    21001 gtgctgacgg ggatgcctcc cagagaggcg ccgataatgg agatggccgc cgcggtttcg
    21061 gcgcagaagc cgccgatggg ctgaagcttg gtaatcttgg tgcccatggt cttcacgatt
    21121 ctccacccgc cgctaatggt tcccagggcg atgacggtgt agcaactgaa gacgacccag
    21181 aaggggatgt gaaaaaccgg gccgatgagc cccttgctgt agagaatgat ggcgatgatg
    21241 cccatggtct tctgggcatc gttcatcccg tggccgaggc tgttgattgc cgaggagcat
    21301 agctgaagtt tacgaaagag tttaatggaa cgggtgacgt tggaattccg gctgagattc
    21361 aggactaggg tcatcatgcc cagtcccagg atcatgccga tgctggggga taaaaagata
    21421 aaaagggcgg ttttcacgat gcccccccag accagtgtcc cgatgccggc cttcagcacg
    21481 ccagcgccga tcaggccgcc gatgagggcg tgggaggaac tgctgggcag gccaagatac
    21541 caggtggtaa gattccagat gatagcaccg cccagagcgg cgaaaataac gtaattgtcc
    21601 atgatgtctg gatggatgat cccgacgccg atggtcttgg ccaccggagt gccgatgatg
    21661 aagatggcgg cgaaattgaa aaacgctgcc cacatcaccg cgaaacgggg ggagaggacc
    21721 cgggtggaga ctactgtgga aatggaattg gcggcgtcgt gaaaaccgtt gatgaagtcg
    21781 aaagccaggg cgatgacaac gaggaaaatt acgaatccga gtgaatcaat catttatgta
    21841 tcagcaaaga accagtgcgg gaaggctcgt cggggttggt gctagccatt ttttaagacg
    21901 atgccttcca cgatgttcga gacatcttcg cagacatcga gagtttcttc gaagcgttcg
    21961 aagatctctt tccatttgat gagttcgatg ggatccgttt cggtttcaaa gagcttggcc
    22021 atggtcgtgc ggaggatctg gtccccggcg ttctcaatcg tgttgatttc cacgcaggcc
    22081 tccaggatca tgggagcgtt tttcatgtcc cgtagtccct ggatgatctt ttttaccttt
    22141 tccgtggcga cgttgagtag tttcgcctgt tccaggagct ccggcgacgg gtacttgatt
    22201 ttgtaaagag acatgcggat cgccgccgct tccgtcatgt ccaggatgct gtccatcttg
    22261 tttaccaggg cgtagatgtc ctccctatcc aggggagtga ggaaggtttt gtgcatcttt
    22321 tcgtaggtcc ggtgggtgat gacgtcggcc tcgtgctcca gttccttgag cttgataagg
    22381 cgggtggcga aggaatcgta gtcgtggagc atatcgagaa agagcttggc cccttcgtcg
    22441 atcttgtcgg ccagttcttc gaagaggtcg taaaactttt cttccctcgg aatgagtcgc
    22501 atcgccgttt cctcccggtg gttccggtta ttgattgccg aatctaatga cgaccatgat
    22561 cgtggcacgt ccggcgtcgt cggagatcaa ccaccagcaa tttgctgaat catctatagg
    22621 attgctcttg aaaatgcaac cggttttcgg catatgtaaa agaaaataaa tagttgcccc
    22681 gccaatgggg ccgcttttcg cttgcgtgga tttgtttttt aggcgatata aattgccggg
    22741 aaagaaagga ggtccgtcat tggtctggga acggaaacga atcctcgtcg tcgatgacga
    22801 aacggatatc gtcgagctga tcgcttacaa tctggccctg gagggctatg aaatcaggaa
    22861 agccggcgat ggggagacgg ccctggcgat catcagggcg gaaaaaccgg cgctgatgat
    22921 cctggacctg atgcttcccg ggatcaaggg gctggacgtc tgccgggcgg tgcgaagaga
    22981 ccgggcgacg gaggcgatcc ccatcatcat gctcacggcg aaaggggagg acacagacaa
    23041 ggttgtcggc ctggaaatgg gggcggatga ctacatgacg aaacctttca gcatccggga
    23101 actgacggcc cgggtccggg cggtattgcg ccgttcggag tcccggcccc accgggaggc
    23161 ggagccggcg gagacggcgg agacctttgc cgccggcgac ctcttcttgg attatgccgc
    23221 ctgtaaggtc gctgtggcgg gaaaaaagat tgaactggga cctacggaat atcgcctgct
    23281 gaccttcttc acccgccatc ccggccgggt
//
LOCUS       DAOSMF010000021        22967 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_2792_length_22967_cov_22.064857, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000021 DAOSMF010000000
VERSION     DAOSMF010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 22967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22967
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2792_length_22967_cov_22.064857"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(668..1174)
                     /locus_tag="PLT63_04120"
     CDS             complement(668..1174)
                     /locus_tag="PLT63_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97944.1"
                     /translation="MTSYATKLIEITEANAEKIAQQWYNNVKLNPRTPAYHTMPEEEA
                     KRQAMYFFKHFGKLFFTDKPFEEAQVLFSRFAAARYGQRIPLSEVIYTLTLMRRHMWL
                     FSSSQAVFITAVEHHQAAESQSRTILMFDYAIYIIAQKYEELLKNELEEKMKGLQIKS
                     HLSFWNKG"
     gene            complement(1284..2642)
                     /locus_tag="PLT63_04125"
     CDS             complement(1284..2642)
                     /locus_tag="PLT63_04125"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOD97945.1"
                     /translation="MKKDAVKKRGDAGQMSTPSGMLLHSIIQGFSIPTFVIGRDHKVI
                     FWNRALEKLSKIPAGEIIGTNKQWRAFYAEERPCMADLIVDGITERIPKWYEGKYEKS
                     SLLEEAYEATDFFPALGDGGLWLRFTAAAIRDVDGQLVGALETLEDVTDRKNFELKQR
                     ETERELFSVIEGSPTPTFVIGIDHKIIYWNRALEKLSRLPAADMIGTNEHWRAFYARE
                     RPCMADLLVNETPEIIEEWYPGVAKSQLLDETYEAEGFFPDLGPNGRWLRFVAVPIRS
                     SQRVLRGAIETLEDITERKAIEQEIIENERRLYSVIQGFSIPAFVIGKDHKVMYWNRA
                     LEKLSNIKAEEVVSTNRQWQAFYAKERPCMADLLVDSALEKIPKWYSGKYCPSELIED
                     AYEATDFFPDLGENGRWLRFTAAVIRDSRGELVGAVETLEDITAQKAPGETAGAAAKK
                     KR"
     gene            complement(2693..3487)
                     /locus_tag="PLT63_04130"
     CDS             complement(2693..3487)
                     /locus_tag="PLT63_04130"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HOD97946.1"
                     /translation="MDKRLCVICAWRQHCQKRFSVASVGMLNIHCPDFTRDYTIKDVE
                     PDKKLVEQQLEKWRQERTAKAKPCITVSREPGAGGSEIARKLAKDLKMDLMGGQIISR
                     VAESTRMSEKVVMTLDEKNVTKLDSWINSLFTSRHLWPDVYLKHLTKVIATIGEHGNA
                     IIVGRGSQFILRPDRIFRVRFIAPFDKRVLRVMKNRNCTSAEAESYLIKTDGGRAAFI
                     KKYFNEDIASPANYDLVVNTAILSCEEAAELVKKAFQSRKFYNNVS"
     gene            complement(3602..4579)
                     /locus_tag="PLT63_04135"
     CDS             complement(3602..4579)
                     /locus_tag="PLT63_04135"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HOD97947.1"
                     /translation="MLTAEALKRKIQSADDLLSVVKTMKALAAVSIRQYQRAVESLSD
                     YNRTVEMGLQIVLKQSVGAFKVGVGSSPRLGAIVFGTDQGLCGQLNSIIVQHALGEMG
                     QTGIPKERRIVIAVGMRTADILEDSGQKVFEILGTPGSTAGITPMVQDITMVLESWRF
                     KHSVENMHLYFNEYISGATYRPQTHKLLPVDRQWLLKLQKKRWDSRTLPMFTIEWETI
                     LRSLIREYLFVSIYRAFANSLASENASRLAAMQNAEKNIEERLEELEVQFHSQRQMTI
                     TEELLDIVAGFEAMKEDQRPVGKSGKTAAREGVAGASLTGGRVVPAALA"
     gene            complement(4566..6167)
                     /locus_tag="PLT63_04140"
     CDS             complement(4566..6167)
                     /locus_tag="PLT63_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternate F1F0 ATPase, F1 subunit alpha"
                     /protein_id="HOD97948.1"
                     /translation="MNSVSTEQENLKTFLEDTFRTMDAVLDGQEAELRDREVGVVTYV
                     GYGIVRVAGLPNIRADELVLFPHNLQGLVFNIDPEELGVILLGPSEDLSAGAEVRRTG
                     RVLDTPVGESLIGRVVDALGRPLDNLGEIRTVKRLPVEREAPPVMARDPVTVPLQTGI
                     KVVDALFPIGRGQRELIVGDRQTGKTAITIDTVINQHDKDVLCIYCSIGKKASDTAKV
                     IADLKQYNAMSYTITVIATGDDPPGLQFIAPYAATSMGEYFMSQGRDVLIIYDDLTYH
                     ARAYRELSLLLRRPPGREAFPGDIFYIHSRLLERSTHLRKEHGGGSLTSLPIVETEAQ
                     DISGYIPTNLISITDGQLYLSPRLYQKGILPAIDVGKSVSRVGGKAQLPAYRAVASDV
                     RISYSQFEELETFSRFGTRLDEATRKVLERGWRVREILKQPQYKPIPVAEQVAAILGV
                     TSGMFDSVAIGEIRAAEETLRRNVMETHGGLCGRIASGEKLGDTERELLIHAAGETAR
                     PYVGKEAGEETIGGAFGKGADHADR"
     gene            complement(6180..6947)
                     /locus_tag="PLT63_04145"
     CDS             complement(6180..6947)
                     /locus_tag="PLT63_04145"
                     /inference="COORDINATES: protein motif:HMM:TIGR03321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97949.1"
                     /translation="MHIDWFVFFSQVVNLMILMLLLKKFLFGRIIKAMDAREAKIAAR
                     FAEAEHSQAEAEKATMACEERLRDLEGRYDEMLNKARQDAEAYRERLLEKAKEEVEFL
                     KTRWIEALRSEQNNFLQELRQLAGNQIYNVTRRVLKDLADLDLEEQIVSVLMERLDEL
                     DEEERRRFSSSGHDGGITVSCAFDISTAMRAKLEQAIRQYAAGEVDIAYERSEDVLSG
                     CELRANGHKLAWSVKDYIDGIEERFYAALYEEARERK"
     gene            complement(6951..7229)
                     /locus_tag="PLT63_04150"
     CDS             complement(6951..7229)
                     /locus_tag="PLT63_04150"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HOD97950.1"
                     /translation="MDIISTVALASIISAGLCMGIGAIGPGIGQGRAVSGALSSIAQQ
                     PDERNSLTRTLFVGLAMIESIAIYCFVISMILVFANPFWTYVIKKAGG"
     gene            complement(7249..7962)
                     /locus_tag="PLT63_04155"
     CDS             complement(7249..7962)
                     /locus_tag="PLT63_04155"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOD97951.1"
                     /translation="MEVMAMYQISPDQVILFTWHFITINATLVYTWLIMGLLVFGSVI
                     VTRNLSTDRTMSRWQNLLEVIVGGIRSEIRDVVREDVDVYFTFIGTLFLFILASNIFT
                     FIPGYVAPTSSLTTTATLAILVFFAVPIYGIAKQGILEYLKEYFRPIFIFFPFHVMGE
                     LSRTLALAVRLFGNLMSHEKVIAILLAVTPLFFPVVMQVLGLLIGLIQAYIFAVLAMV
                     YIASATQSHEEMEHTKQQN"
     gene            complement(7953..8261)
                     /locus_tag="PLT63_04160"
     CDS             complement(7953..8261)
                     /locus_tag="PLT63_04160"
                     /inference="COORDINATES: protein motif:HMM:NF024368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HOD97952.1"
                     /translation="MLSHTEHIIIALGAGMGIGLFYFAGLWRTVRLLCGHPRPFRLLA
                     LSYAGRLLPAMGGFYLLLSDGWLYLAAALAGFILVRTALMKILGKFSFLPWKGAAVWK
                     "
     gene            complement(8329..8658)
                     /locus_tag="PLT63_04165"
     CDS             complement(8329..8658)
                     /locus_tag="PLT63_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HOD97953.1"
                     /translation="MSDEQLTPGERRRKQAEDFTRKVCVKEQRRIKGKNMQDQTVWFG
                     LGMFGIVGWSVAVPSLIGTAVGIWIDQTWPSRFSWTLMLLILGVSLGCLNAWYWVKKA
                     RDNIIKD"
     gene            complement(8651..9037)
                     /locus_tag="PLT63_04170"
     CDS             complement(8651..9037)
                     /locus_tag="PLT63_04170"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HOD97954.1"
                     /translation="MNLKILLPAEVFLVEEVAKVVVEGENGFFCLLPQHVDFTATLAP
                     GVFTFTDMEGDENYLAVDTGALVKRGQEIFVSTRNAVRGPELGRLKEFVVAQYRQIDE
                     REKKARTAAAKLEVDLLRRFMELRHE"
     gene            complement(9136..10527)
                     /gene="atpD"
                     /locus_tag="PLT63_04175"
     CDS             complement(9136..10527)
                     /gene="atpD"
                     /locus_tag="PLT63_04175"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOD97955.1"
                     /translation="MAMKTERNEGTVISVRGSVVDSRFSPGRLPAIHNVLHAGDDGRI
                     RIEVMVQLNNEVVRGISLRPTQGLARGSKVTDTGQPFLIPVGKELLGRVFNVFGEPID
                     NADPPPTTDTRSLHGVPVPITQQSTVSEIFETGIKAADVLCPLERGGKAGLFGGAGVG
                     KTVLLTEMIHNMVGHHHGISIFCGVGERCREGEELYREMKEAGVLPNTVMVFGQMNEP
                     PGARFRVGHTGLAMAEYFRDAERQDVFLLIDNIFRFIQAGQEVSGLMGLLPSRLGYQP
                     TLASDLAALEERICNTTTGAITSIQAVYVPADDFTDPSASHTFSHLTASIVLSRKRAS
                     EGFYPAIDLLQSGSKMLMPNIAGERHYRIAQEIRRNLTIYEELKDIIAMLGINELSRE
                     DQRTVYRARRLERFFTQPFHVTEQFIGKPGKTVSLADSLDGCERILNDEFADAPESAL
                     YMIGSIDEAVRGN"
     gene            complement(10544..11398)
                     /locus_tag="PLT63_04180"
     CDS             complement(10544..11398)
                     /locus_tag="PLT63_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97956.1"
                     /translation="MSGSLKLVNLIESHADSIAWQWARDVKRNPRTPSYHHLAEETLV
                     PMAMRFYDHFRQMFFTDRPADVSQQYFARYAEEHHGRNIPLDEALYALIMMRRHIWLY
                     AEFQTIFISAVEQKQAVDSLVRTILMFDYAIFFISGRYQELIQEGIEEKLGVVNVLRM
                     KNDPGARPESSKVLIMTALIIGAILLTYYNYSVLGGNSIYSHLLYIPVVLAGIWWKKR
                     GIIVAAGLGAYLLMSHLLFMKGMPFMDDMIRTAMFLIIGTIVAFLSEGIAGAERIYRR
                     QPTASRFS"
     gene            complement(11552..12016)
                     /locus_tag="PLT63_04185"
     CDS             complement(11552..12016)
                     /locus_tag="PLT63_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97957.1"
                     /translation="MVASMKLIDLVENHSEKIAAQWSKLVVKNPFTPFYHNKQAERLV
                     PQAVRFYKNFRKLFMEPKPVEASDGYFTSYAEKQYREGVPLHEAVYALILMRRQIWLY
                     AEFQDIFSTAVEKQQAIESITRTMLMFDYAVYFITRRYRELMKLEWFEAPNT"
     gene            12523..13296
                     /locus_tag="PLT63_04190"
     CDS             12523..13296
                     /locus_tag="PLT63_04190"
                     /inference="COORDINATES: protein motif:HMM:NF039354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6125 family protein"
                     /protein_id="HOD97958.1"
                     /translation="MAEEGSTKVITAEKPEDLSQEDMARLIVDMFHRNMVHYTLWFTA
                     VKHQFGITRALEIMKKARKNSYDIQMDRMAKVFGFEMVNGIPKPLLEMPKEKLINIMN
                     NLGVNWLANDGVWFQAVESMRDMYDAKRCNDTCWTRYSPFEAWSIKEFLGLPHEAGLE
                     GLKQALRFRMYARINVQSIIDEGPNSFVFQMNDCRVQSARKRRGLADYPCKSAGLVEY
                     RHFGEYIDNRILCECIGCPPDEHPEEWFCAWRFSIPEGA"
     gene            13362..14399
                     /locus_tag="PLT63_04195"
     CDS             13362..14399
                     /locus_tag="PLT63_04195"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="s-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOD97959.1"
                     /translation="MTAEPLLMHLENNVYLEGEDALIIIDRRKLPRSQAEVYCTDHVE
                     VARAIEQMMVQGAGDIAITAGYGLYLAARETERQRGGRNMAFLKHAADRLCATRPTGF
                     HLAVLLGKLLERVRREPDGRASEIILATLQKSLARQREISQATGRQAETLLTPGDTIL
                     THCFAGAGLLYMFQYARENGKVIKALCTETRPYLQGARLTAWSLSEMGIDTTLITDNM
                     AAWCMSRGMADKVFAAADRIAMDGSAANKVGTLQLAICAWRLDIPFYILGYGGPDSRT
                     LRGSDIPIEERDPREVLEFQGAPISGELVKAYYPAFDVTPPELITGIVTVDGVFSPAE
                     IADGLYENVHK"
     gene            complement(14646..14867)
                     /locus_tag="PLT63_04200"
     CDS             complement(14646..14867)
                     /locus_tag="PLT63_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97960.1"
                     /translation="MDLGDIVMVDNPRHPFYGRRGKIIGRRGLYREDDPWFLIYVPSH
                     MRSFLIPQSMLCLEQDASVTVDIFTERLQ"
     gene            15083..17200
                     /locus_tag="PLT63_04205"
     CDS             15083..17200
                     /locus_tag="PLT63_04205"
                     /inference="COORDINATES: protein motif:HMM:NF024818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD97961.1"
                     /translation="MKNSGADHYRHKREGPLAREELDLLMGCIGGSPIPAFVIDRHHR
                     LIYWNRALEELSAISAAEVIGTDQHWRAFYSSRRPCLADLLVEGTVADIPRWYGRKYI
                     KSPLIDEAYEATDFFPELGDSGRWLRFTAALLRAPQGPALGAVETLEDITERKAAEEA
                     LLKAHEELEARVRERTLELGRTNEILQRTTDQLSLLLESLPIVSYTRNAVERYEITFV
                     SHSVTEITGYHPGDFTDKHTFWQERLHPDDRRRVIKSLQETGGAGTHHCEYRFRAVDQ
                     TYRWFSDFWRPVRMPDVPTPHFVGIWQDVTEEKRIRRENELRLQQIIQTHKLTALGEV
                     VAGVAHEINNPISFISYNIPLLEEIWSTIAPVLKDHDDFARSCSRRGLGPGEVMDNMD
                     QIIQAFKIASTRISRVITGLKEFSRSDERSEKKTLAVNEVIRGALVIVGAQVRKTVSI
                     IEERLAVDLPPVQGHFQKLEQVIGNLLINAHQSMPEDRKGKITITTCHLPRLDAVVIA
                     IEDTGRGIAREALGHVFDPFFTTRRDSGGTGLGLSISYGLIKEHGGTIGVLSHPRLGS
                     RFSIFLPVEGKQLPRLQPRLLLIDADASFLKDLQAHFVAAATWLIKSPADKEGIVRFL
                     DETPEVDWVIAAADLRILTGEEVIAAVRERHPLLYTVLYGPGGRSRRHGLPPACPPSF
                     TVAKPFAMEELVKLIEDTGRQRL"
     gene            17197..18582
                     /locus_tag="PLT63_04210"
     CDS             17197..18582
                     /locus_tag="PLT63_04210"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOD97962.1"
                     /translation="MNILIVDDEEIALTSVQRLLKWRGLRDVEICDNGREAILRIREK
                     DYDIVLLDLLMPETDGMQVLEATKPFKPWTEFIILTAVDDIPVTVRAIRLGAYDYLVK
                     PVDNELLFLTIERAYERKGLLKGLSAGSRTYAGETPGAFAEIITQCPLMKSLLAYAQV
                     MARSGNPVLITGESGTGKELVARGIHRAGPAPNGPFIAVNVSAIPNTMFETQFFGHVR
                     GAFTGAEANYRGFFEQADGGTLFLDEIGELPAGLQAKLLRVLEEKSFTPLGGSKAIQV
                     DVRVISATNMDLDRACQEGRFRLDLLYRLQSAHVHIPPLREREGDIALLANHFLREAN
                     NRHGKEVTGFSPEAMDVLTRREYRGNVRELAQVVENSVLLCEREIILPHHLGRQPTRQ
                     SLFARRLCSLKENDEIQVAYVLMNTGPDRRQAAAVLGITVRQLQRKLAQMREDPYWRE
                     VMGEALSRERD"
     gene            18610..18912
                     /locus_tag="PLT63_04215"
     CDS             18610..18912
                     /locus_tag="PLT63_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97963.1"
                     /translation="MNKKDFTSLDYPLFEAMRKKAPQAARRWLLGDRLFQVGLLLTIM
                     FLPLVFFVYTQWGSGLFLIGMGVGLACSLGLLAAGVYYKRESYKLAIKAGIDVTSL"
     assembly_gap    19076..19175
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<19176..19996)
                     /locus_tag="PLT63_04220"
     CDS             complement(<19176..19996)
                     /locus_tag="PLT63_04220"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HOD97964.1"
                     /translation="MKQISWEDYGSLKSFFKCQPYGLCSYSLPSLIVWSNHVFQTSYT
                     QENGRVIIANDCRHRPEDSHLLLPLLPEGLPGPEELAALAARTGYHRYESVPEDYLDR
                     TGWDRVSRYFAITAHPEYDDYIYLTDDLSRLKGNRYARKRNLLHQFDRNYLAVGRVAA
                     GSITPAVVPECLVCLEKWCAIKKCDVDQDLDMACEKKAAINALLHLDKLEMRSLFIRI
                     DGEVCAFCISSHLGPDMGVMNFEKALPHIRGLYQYLDNECAKTLFPGYRYINKES"
     gene            complement(19993..20436)
                     /locus_tag="PLT63_04225"
     CDS             complement(19993..20436)
                     /locus_tag="PLT63_04225"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOD97965.1"
                     /translation="MTLTFIDDPDGKQVAAIFRLYETAGWWTGRTRESPDLVRRIVTG
                     SHCFVLAEEGKEIVGMGRAISDGVSDAYLQDVSVRPEFRKRGIGARIVREILLRLQTD
                     GLQWVGLIAGRKSRSFYRHLGFEEMPAATAMLMMINFERALREQG"
     gene            complement(20433..21509)
                     /locus_tag="PLT63_04230"
     CDS             complement(20433..21509)
                     /locus_tag="PLT63_04230"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOD97966.1"
                     /translation="MEAKPNSHNPGKTRHAIFYLLLGLATLLLLYLLKPFFYPLFWAA
                     VIAIVFKPFHRRLGARIKSPSLTAVASLLLIMTVIILPAGMVGSLLLNESFHVYNSLN
                     VDGARIESQINDIIARIAADPYLRYFHIDMTLIGKNVTALVEEATSFIFDSLKNLTQN
                     TIMFLVNFVIMLYALFFFLRDGEKFVHMTMRAFPLGAAKEGLLLERFAAMTVATLKVT
                     LIIGGLKGILGSVVFFLTGVHGAITWGVLMVFTSIIPAVGCAIVWGPVGVVMIILGHV
                     WEGLIILLCGFTIISHVDTFLRPLLVGQAIRMHTLLIFLSTLGGLSLFGISGFVIGPV
                     IVSLAQTLWEFHDELLKINNGLQS"
     gene            21668..>22967
                     /locus_tag="PLT63_04235"
     CDS             21668..>22967
                     /locus_tag="PLT63_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97967.1"
                     /translation="MIELWNDTSPILVTCARGITPYLREEILALGLPVREVSEAAVET
                     AGAMTDAMRLNLVLRTGQRVLFLLHEGMARSPGDLYAGAVDLPWEEVLHVDRYFCVTS
                     AVDTPEIRDPRYANVRVKDAVVDRLRGLFRRRPDSGSDRTGAVVHLYWQGDRYRIFAD
                     SSGEPLSRRGYRKIPLTAPMQETLAAAALMATGWTGEETLINPMCGSGTLAIEAALIA
                     RRRAPGLLRENFGFFHLRGFDDERWQSLRVRVRKEERKEIPGRIIATDISPEAVRGAA
                     INAGRAKMGRHIQFRVCPFAATPVPQSPGVVIMNPPYGLRLGEEEELGRLYGEMGDFL
                     KTKCGGYRGYIFTANLELAKKVGLRTARRLIFFNGELESRLLEYPLYEGSKKQAPGAE
                     ESAAVREAVEESNAADAVTPAAAKSYARIVRPSVPRMIRRR"
BASE COUNT         4820 a         6773 c         6506 g         4768 t
ORIGIN      
        1 cagatcggac acggtggacc agcgcggttt ctgatcggga ttcttggcga cgaccttgcg
       61 gacctcgtcc tcccacttct gcctcatcgt actggatttt tccagcactt ccgctgcaaa
      121 gtacatacgt tacaccctcc ttactttaaa atgtttcatt gaacctttaa gagacctgat
      181 cataaaacct ttccaaattc ctggatatca taatactttt atgatacctg atcatgaccc
      241 ccctgcacga gaattatccc ggcgtcatga acaagttgca ggcaagtgaa tgaaccattg
      301 gaggagcgta tttaaaggat tccgcctcat tgtccggcta tttatatatg ataaaggccc
      361 tgaagcgcgc ttttaaaccg aatgttctga tattacgatg ccacaatttt gtcaaggaca
      421 aaaagccggc gcggcttcat gatgagccgg gaaactgcgc cggtattcag gggaggaatc
      481 ttattccacg gtgggtgacg aatattttag cggggagtga taatgcaatg aaaacgggtg
      541 ggcaagggaa aggatttccc tagccggggg gcgcccgcat tgcgttatgg atcaggatgg
      601 ggcatcggcc ccccgaagcg agggagaaaa ttcgtgtttt gacgaatcct tgcttgcaac
      661 cgcagcatca acccttgttc cagaaggaca ggtgactttt gatctgaagt cctttcatct
      721 tctcttccag ctcgtttttc aggagttcct cgtatttctg ggcgatgatg tagatggcgt
      781 aatcgaacat gaggatggta cggctctgac tttccgcggc ctgatgatgt tccaccgccg
      841 tgataaaaac cgcctgggaa gaggaaaaca gccacatgtg cctcctcatc agggtcaggg
      901 tatagatgac ctcggatagg gggatgcgct ggccgtaacg ggccgcggcg aaccgggaaa
      961 aaaggacctg ggcctcttcg aagggtttgt cggtgaaaaa gagcttcccg aaatgcttga
     1021 agaaatacat ggcctgccgc ttggcttcct cttccggcat ggtatgatat gctggggtgc
     1081 ggggattcaa ttttacatta ttataccact gctgggcgat cttttcggcg ttggcctcgg
     1141 tgatctctat caatttggtg gcgtatgatg tcatggtctg accctccttc tatgcttgtg
     1201 aaaatgcgga tcttcccaaa tcactggcgg tgattcttgc cataaatctt tgaaccgggt
     1261 tttcccgttt tggccgcccg agatcatctt ttctttttag ccgccgcgcc agccgtttcc
     1321 cccggcgcct tttgcgccgt aatgtcctcc agagtttcca cggcaccgac caattcgcct
     1381 cgggagtccc ggatcaccgc cgcggtgaag cgcagccacc gcccgttttc gcccaagtca
     1441 gggaagaagt ccgtcgcctc ataggcatct tcgatcagtt ccgacgggca gtacttcccc
     1501 gaataccatt tgggaatctt ctccagggcg ctgtccacaa ggaggtcagc catgcaaggc
     1561 cgctctttgg cgtagaaggc ctgccattgc cggttcgtgc tgaccacttc ttccgccttg
     1621 atattgctaa gtttttccaa agcgcggttc cagtacatta ccttgtggtc cttgccgatt
     1681 acaaaggccg gaatggagaa gccctggatg acactgtaga gacggcgctc gttttcaatg
     1741 atttcctgct cgatggcctt ccgttccgta atatcctcca gtgtttcgat ggccccccgg
     1801 aggacccgct gggaactgcg gatcggcacg gcgacgaatc tcagccaccg cccgttcggc
     1861 cccagatcgg ggaaaaagcc ttccgcctcg taggtctcgt caagaagttg ggactttgcc
     1921 acccctggat accattcctc tattatttct ggagtttcgt taaccagaag atcggccatg
     1981 cagggccgct cccgggcata gaaggcccgc cagtgttcat tagtgccgat catgtccgca
     2041 gcgggaagtc ggctgagttt ttccaacgcc cggttccagt agatgatctt gtgatcgata
     2101 ccgatgacga aggtaggcgt cggtgatcct tcgatgacgc tgaacagctc cctttccgtc
     2161 tcccgctgtt tcaattcgaa atttttccga tcggttacat cctccagggt ttccagcgcc
     2221 ccgacaagtt gcccgtccac atcccggatg gccgccgcgg taaagcgcag ccacagaccg
     2281 ccgtcgccca aggcaggaaa gaaatccgtg gcctcgtatg cctcttccag aagcgaggat
     2341 ttttcgtatt ttccctcata ccacttgggt atccgctccg ttatcccatc gacgatgaga
     2401 tcggccatac aaggccgctc ctcggcatag aaggcccgcc actgcttatt ggtgccgatg
     2461 atctccccgg ccggtatctt gctgagtttt tccagcgccc ggttccagaa gatgaccttg
     2521 tgatccctgc cgattacaaa ggtggggatg gaaaagcctt gaatgatact gtgcagcaac
     2581 atccccgaag gcgtggacat ctgcccggcg tcacctcttt tcttgaccgc atcttttttc
     2641 ataaggacct ccttgcctgt gataaaaaaa ttgccttgcg ggtcctgata ctttacgaaa
     2701 cattgttata gaacttcctg gactgaaagg cctttttgac tagttccgcc gcttcttcac
     2761 aggacaggat cgccgtgttg accacgaggt cgtaattggc cgggctggcg atatcctcgt
     2821 tgaaatactt tttgatgaag gccgcccggc cgccgtccgt cttgatgagg tagctttccg
     2881 cctcggcgct ggtgcagttg cgattcttca tcacccggag cacccgcttg tcgaaggggg
     2941 cgatgaaccg gacccggaag atccgatccg gtcgcaggat gaactgggac ccccgcccga
     3001 cgatgatggc gttgccgtgc tcgccgatgg tggcgatgac cttcgttagg tgcttcagat
     3061 agacatccgg ccagagatgg cgggatgtga acaaagagtt gatccaactg tccagtttgg
     3121 tcacgttctt ttcatccagg gtcatgacga ccttctcgct catccgggtg ctctccgcca
     3181 ctcgagagat gatctgaccg cccatcagat ccatcttcag atccttggcc aatttcctag
     3241 caatttcgct gcccccggcc cccggttccc gggaaacggt aatgcatggc ttggccttgg
     3301 ctgttctctc ctggcgccat ttttccaact gctgttccac aagctttttg tccggttcca
     3361 cgtccttgat tgtgtagtct ctcgtaaaat ccggacaatg gatattgagc atcccgacgc
     3421 tggcaacgga gaatcgtttc tggcaatgtt gacgccaggc gcagattaca catagtcttt
     3481 tatccattcc cataacctca cccccttaat tgacatgatc ctccaacatc caccaacgat
     3541 gaaatcagcg aagctgattc ttgtttataa aatgtcgtta aggcgactac cggccgtatt
     3601 ttcatgctaa cgcggcaggc acaactcttc caccggttaa tgacgcaccg gcaacgcctt
     3661 cccgggctgc cgtcttgccg cttttcccga ctggacgttg atcttccttc atggcctcga
     3721 aaccggccac gatatcgagc agctcttcgg tgatggtcat ctgacgctga ctgtggaact
     3781 ggacttccag ttcctccagg cgctcctcga tattcttctc cgcattttgc atggccgcca
     3841 gccggctggc gttttcactg gccagcgaat tggcgaaggc ccggtagatg gatacaaaga
     3901 ggtactcccg aatcagggag cggaggatcg tttcccattc gatggtgaac atgggcaacg
     3961 tcctcgaatc ccagcgcttc ttttgcagct tgagcagcca ctgccggtcc acgggaagca
     4021 gtttgtgggt ttggggacgg taggttgccc cagaaatata ctcattgaaa tagagatgca
     4081 tgttttccac ggaatgtttg aaacgccagc tttccaggac catggtgata tcctggacca
     4141 tgggggtgat gcccgccgtg gagccggggg tgccgaggat ctcgaaaacc ttctgtccgg
     4201 agtcctcaag gatgtcggcg gtcctcattc ccacggcaat gacgatccgg cgctctttcg
     4261 gtatgccggt ctgacccatc tccccaaggg catgctggac gatgatgctg ttgagttgcc
     4321 cgcaaagccc ttgatccgtg ccgaatacaa tggcccccag gcgtggagaa ctcccgactc
     4381 ccaccttgaa ggcgccgacg ctctgcttga ggacaatctg caaccccatc tccaccgtcc
     4441 ggttgtaatc ggacagggat tccacggccc gctgatactg gcggatgctc accgccgcca
     4501 gggctttcat ggtcttgacc acactgagga ggtcatcggc gctctggatc tttcttttca
     4561 gggcttcagc ggtcagcatg gtcggccccc ttcccgaaag ccccgccgat ggtctcctcc
     4621 ccggcctctt tgccgacata aggccgggcg gtctcgccag cggcatggat aaggagctcc
     4681 cgctccgtat cacccaactt ttctcccgag gcgatccggc cgcacaaccc gccatgggtt
     4741 tccatgacgt ttctccgcag ggtctcttcc gccgctctta tctccccgat ggcgacacta
     4801 tcgaacatgc cggaagtgac gcccaggatc gccgctacct gctccgccac gggaatcggt
     4861 ttgtactgcg gctgcttgag aatctcccgg acccgccagc cccgctccag gaccttgcgg
     4921 gtcgcctcgt cgagacgggt gccgaaacgg gaaaaggtct ccaattcttc gaactgggaa
     4981 taggagatcc gcacgtcgct ggctaccgcc cgataagcgg gaagttgggc cttgccgccc
     5041 acccgggaca cggatttgcc cacgtcgatg gcgggaagga tccccttttg atagagccgg
     5101 ggtgaaagat agagctgccc gtcagtgatg gagatgaggt tcgtggggat ataaccggag
     5161 atatcctggg cctccgtctc cacgatgggc agggaggtca gcgacccgcc gccgtgctct
     5221 ttccggagat gggtggatcg ctccaggagc cgggagtgga tgtaaaagat gtcgccagga
     5281 aaggcctccc ggcccggtgg ccggcggagg aggagggaga gttcccggta ggcccgggca
     5341 tgataggtca ggtcgtcata gatgatcagg acatcccgcc cctgagacat gaagtactca
     5401 cccatggacg tggcggcgta aggggcgatg aactggagcc ccggcggatc gtcgcccgtg
     5461 gcgatgacgg tgatggtata ggacatggca ttgtactgtt tgaggtcggc gatgaccttg
     5521 gccgtatcgg acgccttctt cccgatgctg cagtagatgc acaggacatc cttgtcgtgc
     5581 tggttgatca ccgtgtcgat ggtgatggcc gtcttgcccg tctggcggtc gccgacaatg
     5641 agttcccgct ggcccctccc gatgggaaag agggcgtcaa cgaccttgat gcccgtctgg
     5701 agcggcacag ttacggggtc ccgggccatc acgggcgggg cctccctctc cacgggcagg
     5761 cgtttcaccg tccggatctc accaaggttg tccaaggggc gtcccagggc atcgacgacc
     5821 cggccgataa gggattcccc tacgggggtg tcgaggaccc gtccggtgcg ccgcacttcc
     5881 gccccggcgg aaagatcctc gctgggaccg aggaggatga cccccagttc ctcgggatcg
     5941 atattgaata ccagtccctg gaggttatgg ggaaacagca cgagttcgtc ggcccggata
     6001 ttcggcagcc cggccacccg gacgatgccg taccccacat aggtgaccac ccccacctcc
     6061 cggtcccgga gctccgcctc ctgtccatcc aggacggcat ccatggtccg gaaggtgtct
     6121 tccagaaagg tcttcaggtt ttcctgctct gtggatacgc tattcattat catttcacct
     6181 catttcctct cccgggcctc ttcataaaga gcggcataga accgctcctc gatgccgtcg
     6241 atgtagtcct tcacgctcca ggccagcttg tggccattgg cgcggagttc gcaccccgaa
     6301 aggacgtctt cggagcgctc gtaagcgatg tccacctccc cggcggcata ttgacggatc
     6361 gcctgttcaa gtttcgcgcg catcgccgtg gagatgtcaa aggcgcagga aacggtaatc
     6421 ccaccatcat ggcccgaaga ggagaaacga cgccgctctt cctcatcgag ctcgtccagg
     6481 cgctccatga gcacggagac gatctgctcc tcgagatcga ggtcggccag atccttaagg
     6541 acccggcggg taacgttgta gatctggttt cccgccaact gccggagttc ctggaggaag
     6601 ttgttctgtt ctgaacgcag ggcctcgatc cagcgggtct tcaggaattc cacctcttcc
     6661 ttcgcctttt cgagcagtct ttcacgatag gcctccgcat cctggcgcgc cttattgagc
     6721 atttcgtcgt aacggccctc caggtcccgc aacctctctt cacaggccat cgtcgccttc
     6781 tcggcctccg cctgggagtg ttccgcctcg gcaaaccggg cggcaatttt ggcctcgcgg
     6841 gcatccatgg ccttgatgat ccttccaaaa agaaatttct tcaacaaaag cataaggatc
     6901 atgaggttga cgacttgcga aaaaaagaca aaccagtcga tgtgcatgga ttatcctcct
     6961 gccttcttga tcacgtaagt ccagaacggg ttggcgaaga cgagaatcat ggagatgacg
     7021 aaacagtaga tggcgatgga ttcgatcatc gctaagccga caaagagggt ccgggtgagg
     7081 gaatttctct cgtcaggctg ctgggcgatg gagctcagcg cccccgatac ggcccgcccc
     7141 tgaccgatcc ccggcccaat tgcgccgatc cccatgcata accccgcaga tatgatcgat
     7201 gccagtgcta cggtgctgat gatatccata atctctaacc tccctttctt agttttgttg
     7261 ttttgtatgt tccatttcct cgtgggattg ggtcgccgag gcgatgtaaa ccatagccag
     7321 gacggcaaat atataggcct ggatcaagcc gatgagcaat cccagaacct gcatcaccac
     7381 ggggaaaaac aggggcgtaa cggcgagaag gatcgcgatg accttctcgt ggctcataag
     7441 attgccgaag agccggacgg ccagagccag ggtccgggac agctcgccca tgacatgaaa
     7501 ggggaaaaaa atgaaaatcg ggcggaaata ttccttcagg tactccagga ttccctgttt
     7561 ggcgatgccg taaatcggca cggcaaagaa gaccagaatt gccagggttg ccgtagtggt
     7621 cagggacgac gtcggggcca cgtaaccggg tatgaaggtg aagatattcg aggccagaat
     7681 gaatagaaaa agggtcccga taaaggtgaa atagacgtcc acgtcttccc ggacaacatc
     7741 cctaatctcg gagcggatgc cgccgacgat cacctccagg aggttctgcc aacgggacat
     7801 cgtccggtcc gtggagagat ttctcgtcac gatcacggaa ccgaacacca aaagacccat
     7861 gatgagccag gtatagacga gggtggcatt gatggtgatg aaatgccacg taaagaggat
     7921 cacctggtcg ggactgatct ggtacatggc cattacttcc ataccgccgc tcctttccag
     7981 gggagaaacg agaactttcc cagtatcttc atcagcgcgg tccggaccag gataaaaccg
     8041 gccaaggcgg cagccagata cagccaacca tcgctcaaga gcaggtaaaa cccacccatg
     8101 gccggcaaca gtcggccggc gtagctaagc gccagcaagc ggaagggccg ggggtgtccg
     8161 cacagaagcc gcacagttcg ccatagaccg gcaaagtaga acagaccgat gcccatcccg
     8221 gcgcccaggg caataattat atgttccgta tggctcaaca tggcttcctg ttgcctcctc
     8281 accccccccg gacggcacgt ctcggaaaag ggacggttca ccgccgtctc aatccttgat
     8341 gatgttgtcc cgggcctttt tcacccaata ccaggcatta aggcagccca aagagactcc
     8401 caggataagg agcatgaggg tccaggaaaa gcgggagggc caggtctgat cgatccagat
     8461 cccgaccgcc gttccgatca gggagggaac ggccacggac cagcccacga tgccgaacat
     8521 acccaagccg aaccagacgg tctggtcttg catgtttttc cccttgatgc gccgttgctc
     8581 cttgacacag accttccggg taaaatcctc cgcctgtttg cgcctgcgct ctcccggcgt
     8641 caactgttcg tcactcatgt ctaagctcca tgaatctccg cagcaggtcc acttccagct
     8701 tggccgccgc ggtgcgggcc tttttctccc gttcgtcgat ctgccggtac tgggcgacta
     8761 caaattcctt cagccgcccc agctccggcc cccggacggc gttccgggtg gaaacgaaga
     8821 tctcctgtcc ccgcttaacc agggcacccg tatccaccgc cagatagttt tcatcccctt
     8881 ccatgtcggt gaaggtgaac acccccgggg ccaaagtcgc tgtaaagtcc acatgctggg
     8941 gcagcaggca gaaaaacccg ttctcccctt caacgaccac cttggctacc tcttccacga
     9001 ggaacacctc tgccggcagc agtatcttca ggttcatccc acctcatccc cctcccggcc
     9061 acatcttggc aacccgcccg tataacacct aatatcctta gggacggccc cttttcgaat
     9121 cctcccccac ccagttcaat tccccctgac cgcctcgtcg atggacccga tcatatacag
     9181 ggcgctctcg ggggcatcgg caaattcgtc attcaagatc cgctcgcacc catccaggga
     9241 gtcggccagg gagaccgttt tccccggctt gccgataaac tgctccgtca catggaaggg
     9301 ctgggtaaag aacctttcca ggcgccgggc ccggtaaacg gtccgctggt cctcccggga
     9361 gagttcgttg atccccaaca tggcaatgat atccttcagt tcctcataaa tggtaagatt
     9421 gcgcctgatc tcctgggcga tccggtaatg ccgctctccg gcgatgttgg gcatgagcat
     9481 cttcgacccc gactggagga gatcgatggc cggatagaag ccctcgctgg cccgcttccg
     9541 tgacaggacg atggaggccg tcagatggct gaaggtatgg cttgccgaag gatcagtaaa
     9601 atcgtccgcc gggacataaa cggcctggat ggaggtgatg gccccggtag tggtattgca
     9661 gatccgctcc tccagggccg ccaggtcgga agccagggtc ggctgatagc cgaggcggga
     9721 gggaaggagc cccatgagcc ccgaaacctc ctggccggcc tggatgaagc ggaagatgtt
     9781 gtcgataaga aggaagacgt cctgccgttc cgcatcccgg aaatactcgg ccatggcgag
     9841 ccctgtgtgt ccgacgcgga aacgcgcccc gggaggttcg ttcatctgtc cgaagaccat
     9901 tacggtattg ggcaggacgc ccgcctcctt catttcccgg tagagctcct ccccttcccg
     9961 gcaccgctcg ccgacaccgc aaaagatgct gatcccatga tgatgaccca ccatattgtg
    10021 gatcatctcg gtgagcagca cggtctttcc caccccggcc ccgccgaaaa gccccgcctt
    10081 gcccccccgt tccagggggc agaggacgtc ggcggccttg atgcccgttt cgaagatctc
    10141 cgacaccgtg gattgctggg tgatgggaac gggaacgccg tgcagtgacc gggtgtcggt
    10201 cgtaggaggg ggatcggcat tgtcgatggg ctcgccgaag acattgaaga cccgcccgag
    10261 gagttccttc cccacgggaa tgaggaaggg ctggcccgta tctgtcacct tcgatcccct
    10321 tgccaatccc tgggtaggcc gcagggagat gccccgcaca acctcgttat tgagctgcac
    10381 catgacctcg atacggatcc ggccatcgtc tcccgcatgg aggacattgt ggatggcagg
    10441 caagcgcccg ggagaaaagc gggaatccac cacgctgccc cgtacggaga tcactgttcc
    10501 ttcgttacgt tccgttttca tcgccatact cccccttctt acctcatgaa aacctggaag
    10561 ccgtcggctg ccgcctgtaa atcctttccg ccccggcgat cccttcgctg aggaaggcca
    10621 caatcgttcc gatgatcaga aacatcgcgg tacggatcat atcgtccatg aagggcatcc
    10681 ctttcatgaa aagcaagtga ctcatcaaca aatatgcccc cagcccggcg gcgacgataa
    10741 ttcctctttt cttccaccag atccccgcga ggaccacagg gatgtaaagc aagtgggaat
    10801 agatggaatt tccaccgagg accgagtaat tgtaatatgt caagaggatc gccccgatga
    10861 tcaaggccgt catgatgaga accttcgatg actccggtcg agccccgggg tcgtttttca
    10921 tgcgcaggac gttcacaacg ccgagtttct cctcgatacc ctcctggatc aactcttggt
    10981 aacgcccgct gatgaaaaaa atcgcataat cgaacatgag aatggtgcgg accaggctgt
    11041 ctacggcctg cttctgctcc accgcggaga tgaagatggt ctggaactcg gcgtagagcc
    11101 atatatggcg ccgcatcatg atcagggcat agagggcctc gtcgaggggg atgttccggc
    11161 catggtgctc ttcggcgtac cgggcgaagt attgctggga gacatccgcc ggccggtcgg
    11221 taaagaacat ctgccggaag tggtcataga agcgcatggc catcggcacg agcgtttcct
    11281 ccgcaaggtg atgatacgaa ggggtccggg gatttctctt cacgtcccgg gcccattgcc
    11341 aggcgatgga gtcggcgtga gattcgataa gattcacgag tttgagcgaa ccggacatag
    11401 tttccgccct cctgtgactc cagtactccc gcaggggaag gcctgcggtt gccggcgctc
    11461 ctttttcggt tccggccccc gggccttttc cgctgtcgcc cccttgccgg ctcgaccccc
    11521 tcgccgcggc ggcaaatggc tgtccacacc ctcaggtatt cggcgcttca aaccactcca
    11581 gcttcatcaa ttcccgataa cgtctggtaa tgaaatacac ggcgtaatcg aacatgagca
    11641 tggtgcgggt gatgctctcg atggcctgct gcttttccac ggcagtggaa aaaatgtcct
    11701 gaaattctgc atagagccat atctgccgcc tcatgaggat gagggcgtaa acggcctcgt
    11761 gcaggggaac cccttcccgg tactgcttct cggcgtaact ggtgaaatat ccgtcggagg
    11821 cttccaccgg cttcggctcc ataaacaact ttcggaaatt cttgtagaac cgcactgcct
    11881 gagggaccag tctctccgcc tgcttgttgt gataaaaagg agtaaaggga ttcttcacca
    11941 ccagcttgga ccactgggca gcaatctttt ccgagtgatt ctcgaccaga tcgatcaatt
    12001 tcatcgatgc taccatcttg gatccctccc ttcataaaaa tttcccgcgt tgaattactg
    12061 tatcgcaaga gccatgccat atccccaggg atgtcgcttg tttaactgct tgaatatata
    12121 cattaattta ccgacagccc ctcgtcagat tccaaggcat gaagaaaatg tcgccccagt
    12181 tcggacacaa cacggccgca attctttccg ttgggacata agtgaatgat tttcattact
    12241 tacacatggc gacacaaatg tcgccggaag atgacaggca ggtcgccagg atcgccccgg
    12301 gaaatccacc gtgttccctt gtttagaaat catcatgacg gtctccgtca caaaggggga
    12361 tgaaaattgt tttattgaca ttcgccctcc cttcccttta atatgccacc atactgccat
    12421 gtacattccg acagacaaaa tgcacgtttt tccatcatca aaggcaattt aggggggaat
    12481 ccccggcggc ggggaaaaat atattgaagg agggaaacag taatggcgga agaaggatcg
    12541 acaaaggtca tcacggcgga aaaaccagag gatttgagcc aggaggacat ggcgcgtcta
    12601 atcgtggaca tgttccatcg caacatggtg cattacacct tgtggttcac agcggtaaag
    12661 catcagttcg gcatcacccg ggcgctggag atcatgaaaa aggctcgtaa gaacagctat
    12721 gatatccaga tggaccggat ggccaaggtc ttcggattcg aaatggtcaa cggcattccc
    12781 aagccgctcc tggaaatgcc gaaagaaaaa ctcatcaata tcatgaacaa tctgggggta
    12841 aactggctgg ccaacgacgg tgtctggttt caggccgtgg aatccatgcg ggacatgtac
    12901 gacgccaaac gctgcaacga cacctgctgg acaagatatt cccccttcga ggcctggtcc
    12961 atcaaggagt tcctcgggct gccccatgag gcgggactgg agggactgaa acaggccctg
    13021 cgcttccgga tgtacgcccg gatcaatgtc cagtccatta tcgatgaggg ccccaacagc
    13081 ttcgtctttc agatgaatga ctgccgggtc caatcggcga gaaaacgccg gggattggcg
    13141 gattacccct gcaagtccgc gggactggtg gaataccgcc attttgggga atacatcgac
    13201 aaccggatcc tctgcgaatg catcggctgt cccccggacg aacatcccga ggaatggttc
    13261 tgcgcctggc gcttcagcat cccagagggc gcataagtcc tcatttttca atttatattg
    13321 gcgccgggac ggcaaggaac gccggcccgt cgggaggagc gatgactgcc gaacctttgc
    13381 tcatgcatct ggaaaacaac gtctatctgg aaggggaaga cgccctcatc atcatcgacc
    13441 ggcgcaagct cccccggtcc caagcggagg tttactgcac cgatcatgtg gaggtggccc
    13501 gggccatcga acagatgatg gtccagggcg ccggggatat cgccatcacg gcgggatacg
    13561 gcctctatct ggccgcccgg gagacggaaa ggcaaagagg cggtcgcaac atggcctttc
    13621 tcaagcatgc cgccgaccgc ctgtgcgcca cccggcccac gggctttcac ctcgccgtcc
    13681 tcctgggaaa actcctggag cgggtccggc gggaacccga cggccgggcc tcggagatca
    13741 tcctggccac cctgcaaaag agtctggccc ggcaacggga gatctcccag gcgacgggaa
    13801 gacaggcaga aacacttctg accccgggag ataccatcct cacccactgt ttcgccgggg
    13861 ccggcctcct gtacatgttc caatacgccc gggaaaacgg caaggtgatt aaggcgctct
    13921 gcacggagac ccggccctac ctccaggggg cgcggctcac ggcctggtcc ctgagcgaga
    13981 tggggatcga cacgaccctg atcacggata acatggcggc gtggtgtatg tcccggggca
    14041 tggcggacaa ggtgttcgcc gccgcggacc ggatcgccat ggacggatcg gcggccaaca
    14101 aggtggggac gcttcagttg gccatctgcg cctggcggct cgatatcccc ttctatatcc
    14161 ttggctacgg cgggccggac agccgcacgc tccggggaag cgacatcccc atcgaagagc
    14221 gggacccccg agaggtgctg gaatttcagg gcgctcccat ctccggcgaa ctggtgaagg
    14281 cttattaccc cgccttcgat gtcactcctc cggagctgat tacagggatc gtcactgtcg
    14341 acggggtatt cagtcccgcc gagatcgccg acggtttata tgaaaatgtc cacaaatagc
    14401 cggatatttt catctttcgt tgtgcccgga acgggcatga tggttagcca tggagaccac
    14461 gaggcgaagg caagcgatat gtgccaattt gattatatca ccttatatat ccgtcagata
    14521 ggcttcatgg aagggatatg gagccgcgag agccgcaatg atcgtcaaca atcaatcggg
    14581 acgttttttc actttttgcg aggtcatcaa tcgcaggcaa tcccggtttt taccggcctt
    14641 ccatgctact gaaggcgctc ggtgaagatg tccacggtaa cggaggcatc ctgctccaga
    14701 cagagcattg actggggaat gaggaaactg cgcatgtggg aaggaacgta gatgagaaac
    14761 caggggtcgt cttccctgta gaggccgcgg cggccgataa tcttgcccct ccggccgtaa
    14821 aacggatgcc ggggattgtc caccatgacg atatcgccga ggtccatacc ctgttcctcc
    14881 tggtcggaaa tgttcgtgtg ccagaaactg cacctgtccg caaaaaaatg gtctctgtat
    14941 agtaccttaa caagctttcc ggagaaaagc cactctttct tttgatatga attgtcatgt
    15001 ttgtccaaca cccacgaacg atgaaaatgt ttaggagaga tcgcgcagat gtattttcat
    15061 actaatcggg aggtgttgcc gggtgaagaa tagcggcgcc gatcattacc ggcacaagcg
    15121 ggagggaccg ctggccaggg aagaactgga cctgctcatg ggctgcatag gcggctctcc
    15181 catccccgcc ttcgtcatcg accgccacca ccgcctcatc tactggaacc gggcgctgga
    15241 agagctgagc gccatcagcg ccgcggaggt catcggcacc gaccagcact ggcgcgcctt
    15301 ctacagcagc cggcggccct gtctggccga tctcctcgtg gaagggaccg tcgcggacat
    15361 tccccggtgg tatgggagaa aatacataaa atcgcctctc atcgacgaag cttacgaggc
    15421 gacggacttc ttccctgaat tgggggacag cggccgctgg cttcgcttta ccgccgcctt
    15481 gcttcgggcg ccccagggac cggccctcgg ggccgtggag accttggaag acatcacgga
    15541 gcgcaaggcc gcggaggagg ccctgctgaa ggcccatgaa gagctggaag cccgggtcag
    15601 ggagcgaacc ctcgaattgg gcagaaccaa cgagatcctc cagcgcacca ccgatcagct
    15661 ctccctgctt ctggagtccc tgcccattgt ttcctacacc cgcaacgccg tcgaaagata
    15721 tgaaatcacc ttcgttagcc actccgtaac ggagatcacc ggctaccatc ccggcgactt
    15781 cacggataag cacaccttct ggcaggagcg gctccacccc gacgaccgcc ggcgggtaat
    15841 caagtccctg caggaaacgg gcggggccgg cacccatcac tgcgagtatc gcttccgtgc
    15901 cgtcgatcag acctatcgct ggttttccga tttctggcgc ccggtccgca tgcccgatgt
    15961 ccccacccct catttcgtcg ggatttggca ggacgtgacg gaggaaaaac gaatccgtcg
    16021 ggaaaacgaa ctgcgtctcc agcagatcat ccagacccac aagctcacgg ccctggggga
    16081 agtggtcgcc ggcgtcgccc atgagatcaa caaccccata agcttcatct cctacaacat
    16141 tcccctcctg gaagagatct ggagcaccat cgcgccggtg ttgaaggacc atgacgactt
    16201 cgcgaggagc tgctcccggc ggggtctcgg ccccggggag gtcatggaca acatggacca
    16261 gatcattcag gcattcaaga tcgcctccac ccgcatcagc cgcgtcatca ccggactgaa
    16321 ggaattttcc cggagcgatg aacggtcgga gaagaagacc ctagcggtga acgaggtcat
    16381 ccggggcgcc ctggtcattg tcggcgccca ggtgcgaaaa accgtttcca tcatcgaaga
    16441 acgtctcgcc gtcgatctcc cccccgtcca gggacacttc cagaagctcg agcaggtgat
    16501 cggcaatctg ctcatcaacg cccaccagtc catgcctgag gaccgcaagg gaaaaataac
    16561 gatcaccacc tgccatctgc cccgcctcga tgccgtcgtg attgccatcg aagatacggg
    16621 ccggggcata gcccgggagg ccctgggtca tgtcttcgac ccctttttca ccacccgtcg
    16681 ggattccggc ggcacagggc tgggactgtc gatctcctac gggctgatca aggaacacgg
    16741 gggaaccatc ggcgtccttt cccacccgcg attgggaagc cgcttcagca tcttcctgcc
    16801 cgtagaggga aagcaactgc cgcgcctcca accccgccta ctcctcatcg acgccgacgc
    16861 ctctttcctc aaagatctgc aggcccattt cgtcgcggcc gcgacctggc tgatcaaatc
    16921 tccggccgat aaggagggaa tcgtccgatt tctcgacgag accccggaag tggactgggt
    16981 gatcgccgcc gccgacttgc ggatcctgac gggggaagag gtgatcgccg ccgtgcggga
    17041 acggcacccg ctcctctaca cggtgctgta cggaccggga ggccgtagcc gccggcacgg
    17101 cctcccgccg gcatgccctc cctcctttac cgtcgcaaag ccttttgcta tggaagaact
    17161 cgtaaagctg atagaagata cggggaggca acgcctatga atatcctcat cgttgacgac
    17221 gaggaaatcg ccctcacatc ggtgcagcgg ctgctgaaat ggcgggggtt gcgggatgtg
    17281 gagatctgcg acaacggccg ggaggctatc ctccgcatcc gggagaagga ctatgacatc
    17341 gtccttctcg atctcctgat gccggaaacg gacggcatgc aggtcctgga ggcgaccaag
    17401 cccttcaagc cctggacgga attcatcatc ctcacggccg tggacgacat ccccgtcacc
    17461 gtccgggcca tccgcctcgg ggcctacgac tatctagtca agccggtgga caacgagttg
    17521 ctgtttttga ccatcgagcg ggcctatgaa cgcaagggac tcctcaaggg attgtcggcg
    17581 ggcagccgga cctacgccgg agaaaccccc ggggcgttcg cggagattat cacccagtgc
    17641 cctctcatga agtccctgct ggcctatgcc caggtcatgg cccggagcgg caatcccgtc
    17701 ctgatcaccg gcgaatcagg gacgggcaag gaactcgtgg cccggggcat ccatcgggcc
    17761 ggacccgccc ccaacggtcc cttcatcgcc gtaaacgtca gcgccatccc caataccatg
    17821 ttcgaaaccc agttcttcgg acatgtccgg ggggccttca ccggggccga ggccaactat
    17881 cggggcttct tcgaacaggc cgacggcggg acgctgtttc tagacgaaat cggggaactc
    17941 ccggctggac tccaggcgaa actcctccgg gttctggagg aaaagtcctt cacgcctctg
    18001 ggcgggtcca aggccatcca ggtggacgtc cgggtcattt ccgccaccaa catggatctc
    18061 gaccgggcct gccaggaggg ccggttccgc ctggatctcc tttaccgcct ccagtccgcc
    18121 catgtccaca tcccccccct ccgggagagg gagggcgaca tcgccttgct tgccaaccat
    18181 ttcctccgcg aggcaaataa ccgccatggc aaggaggtca ctggtttcag tcccgaggca
    18241 atggacgttc tcacccgccg ggaataccgg ggcaatgtcc gggagttggc tcaggtcgtg
    18301 gaaaactccg tcctcctctg tgagagggaa atcatcctgc cccatcatct ggggcgacaa
    18361 cccacccggc agtccctgtt cgcccggcgc ctgtgcagcc tcaaggaaaa cgatgaaatc
    18421 caggtggcct acgtcctcat gaacaccggc cccgaccggc ggcaggcggc ggcggtcctg
    18481 ggcatcacgg tgcggcagtt gcagcggaaa cttgcccaga tgcgggaaga tccctactgg
    18541 cgtgaggtca tgggcgaggc actgtcccgg gaaagagact gagccgtttc agtggaaggt
    18601 atggatccca tgaataaaaa agattttacc tctttggatt atcccctgtt cgaggcgatg
    18661 cggaaaaagg ccccccaggc ggcccgccgc tggttgctgg gagaccgcct cttccaggtg
    18721 ggcctgcttc tgaccatcat gtttctgccg ctggtcttct tcgtttacac ccagtggggt
    18781 tccggattgt tcctgatcgg aatgggagtg ggcctggcat gctctctagg tcttttggcc
    18841 gccggggttt actacaagcg cgaatcatac aaactggcca tcaaggccgg catcgacgtg
    18901 accagtttat aatcgctcct taaatagccg cgcggctatt tgcattctag acggtaagcc
    18961 ctgatcttac ggaccgggtg ataggagttt ttggactccg ccagggcggg gagacccatg
    19021 tcgctttcct tgttgatata gcggtagccg ggaaagaggg tcttggcgca ttcgtnnnnn
    19081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntcgct ttccttgttg atatagcggt
    19201 agccgggaaa gagggtcttg gcgcattcgt tgtcgagata ctggtagagg ccgcggatgt
    19261 ggggcagggc cttttcaaag ttcatgaccc ccatgtctgg tcccaggtgg gaggagatgc
    19321 agaaggcgca gacctcgccg tcgatgcgta tgaacaagct ccgcatttcc agcttgtcta
    19381 aatggagcag ggcattgatg gcggccttct tttcacaggc catatccagg tcctgatcga
    19441 cgtcgcattt ctttattgca caccatttct ccaggcagac caggcattcg ggaacgaccg
    19501 ccggcgttat cgaccccgcg gcaacccgcc ccaccgccag ataattccgg tcgaactggt
    19561 ggaggagatt gcgtttcctg gcatagcggt tccccttgag gcgactcagg tcatcggtaa
    19621 gatagatata atcatcgtac tcgggatggg cggtaatggc aaagtaacgg gagacccggt
    19681 cccagccggt ccggtccaaa tagtcctccg gcaccgattc gtaccggtgg tatccggttc
    19741 gcgccgccag ggcggccagt tcttccggcc ccggcaatcc ttccggcaga agtggcagca
    19801 ggaggtgact gtcttcaggc cggtggcggc agtcgttggc gatgatcacg cggccgttct
    19861 cctgggtgta ggaagtctgg aagacgtggt tgctccagac gatgagggac ggcagggaat
    19921 agctgcatag cccgtagggc tgacatttga agaaggactt tagcgatcca tagtcttccc
    19981 aggagatttg cttcatcctt gttcccgcaa cgcacgctcg aaattgatca tcatgagcat
    20041 cgccgtggcg gccggcatct cctcgaaacc cagatgacgg taaaaggagc gggattttct
    20101 cccggcaatc agacctaccc actgcagacc gtcggtctgg aggcgcagga gtatctcccg
    20161 gacaatccgg gcgccaatgc ctcgtttccg gaactcggga cggacggaca cgtcctggag
    20221 gtatgcgtcg ctgacaccat cgctgattgc ccggcccatg ccgacgattt ccttgccttc
    20281 ttccgccagg acaaagcaat ggcttcccgt cacgatccgc cgcaccagat ccggagattc
    20341 tcgggtccga ccggtccacc agccggccgt ctcatagagg cggaagatgg ccgcaacctg
    20401 tttcccgtcg ggatcgtcga taaatgtcag ggtcatgatt gtaacccgtt attgattttc
    20461 aacagctcgt cgtgaaattc ccagagggtc tgggccaggg aaacgatgac cggaccgatg
    20521 acgaacccgg agatcccgaa gagggaaagc ccccccaggg tggaaagaaa gatgaggagg
    20581 gtgtgcatcc ggatcgcctg ccccaccagc aggggccgca gaaaggtatc cacatggctg
    20641 atgatggtga agccgcagag aagaatgatc aggccctccc agacatggcc caggattatc
    20701 atgaccaccc ccacggggcc ccagacaatg gcgcagccca cggcggggat gatggaggtg
    20761 aaaaccatga ggacgcccca ggtgatggcg ccgtggacac cggtgaggaa gaagacgaca
    20821 ctgccgagga tccccttgag gcccccgatg atcaacgtga ccttcaaggt cgccacggtc
    20881 atggcggcga acctctccag cagcagccct tctttcgcgg ctccgagggg gaaggcccgc
    20941 atggtcatgt gcacgaattt ctcgccgtcc cgtaaaaaga aaaagagggc gtagagcatg
    21001 atcacaaagt tcacgagaaa catgatcgtg ttctgcgtca gatttttgag actgtcgaag
    21061 atgaagctcg tcgcctcctc caccaaggcg gtgacgttct tgccgataag ggtcatatcg
    21121 atatggaaat agcgcagata ggggtcggcg gcaatcctcg cgattatgtc gttgatctga
    21181 ctttcgatgc gggcgccgtc cacgttgagg gaattgtaaa catgaaagga ttcgttgagc
    21241 agaaggctgc cgaccatccc ggcgggaagg atgatgaccg tcatgatcaa aagcaaagaa
    21301 gccacggccg tcagactggg ggacttgatc cttgcgccca gacgtcggtg gaaaggtttg
    21361 aagacaatgg cgatcaccgc cgcccagaag agcggataga agaagggctt caatagatag
    21421 agcagcagga gggtggccag gccgagaagg agataaaaaa tggcgtgacg cgtttttccc
    21481 ggattatgac tattaggctt cgcttccatg gctgtatgat tttacccaac tttccccctt
    21541 ttggcaatta ttttctacat tcgacaccta catttgccca tgctcttccg tatgactttt
    21601 tcccgggcgg agtttttttg tcgagctgat ctttgccaaa tgaacctctt tgtgttagac
    21661 aacggtcatg attgaactct ggaacgatac aagcccgatc cttgttacct gcgcccgggg
    21721 gatcacacct tacctgcggg aggaaatcct cgccctgggg ctgccggtgc gggaggtatc
    21781 ggaagcagcc gtggaaacgg cgggcgctat gacggacgcc atgcgcctca atctggtcct
    21841 gcggaccgga cagcgtgtcc tctttctcct tcacgaaggc atggcccggt cccccgggga
    21901 tctctatgcc ggcgccgtcg atctgccctg ggaagaggtg ctccatgtgg atcgctattt
    21961 ctgcgtcacc tccgccgtgg ataccccaga gatccgcgat ccccgctacg ccaacgtccg
    22021 ggtgaaggac gccgtcgtgg accgcctccg cgggcttttc cggcggcgcc cggattctgg
    22081 atcggatcgc accggcgccg tcgtgcatct ctactggcag ggcgaccgtt accgaatatt
    22141 cgccgactcc tccggagaac ccctgtcccg acggggctac cggaagatcc ccctaacggc
    22201 gcccatgcag gagaccctgg cggcggcagc gctcatggcc acgggatgga cgggggagga
    22261 gacattgatc aaccccatgt gcggcagcgg caccctggcc atcgaggcgg ccctgatcgc
    22321 ccggcgacgc gcccccggcc tgttgaggga aaacttcggt tttttccatc tccggggatt
    22381 cgatgacgaa cggtggcaga gtctgcgggt gcgggtgcgg aaggaggagc ggaaagaaat
    22441 tccggggcgg attatcgcca cggacatctc cccggaagcg gtacggggcg ccgcgatcaa
    22501 cgccgggcgg gcgaaaatgg ggcgtcatat ccagtttcgc gtctgtccct tcgccgccac
    22561 tcccgtgccg cagtccccgg gggtcgtgat catgaacccc ccctacggcc tgcgcctggg
    22621 cgaggaggaa gaactgggac gtctgtatgg agagatgggg gatttcctga agacgaaatg
    22681 cggcggttat cgcggctata tctttaccgc caatctggag ctggcaaaga aggtggggct
    22741 acgcaccgcc cggcgactga tttttttcaa cggcgagttg gaaagccggc tcctggaata
    22801 ccccctttac gagggcagca aaaaacaggc cccgggggcg gaagaatcgg cggccgtccg
    22861 ggaagcggtg gaggaatcaa acgcggcgga tgcggtgaca cccgcggcag cgaaaagcta
    22921 tgcccggatt gtccggccgt ccgtcccccg gatgataaga cggcggc
//
LOCUS       DAOSMF010000022        22585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_2851_length_22585_cov_21.926187, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000022 DAOSMF010000000
VERSION     DAOSMF010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 22585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22585
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2851_length_22585_cov_21.926187"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="PLT63_04240"
     CDS             complement(<1..559)
                     /locus_tag="PLT63_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97968.1"
                     /translation="MMKNVIFLSKEELLERLWQADPKIKKILRDSKHIEEARHLLFDY
                     FNYLNRSMFNMKPDTYYSDMNITEKRNARECIRVFSNTVRTENEHLTHASPLQLLYDL
                     AHDPDTALPLASEAFLCEYLAFFRGITGKSGKHSKSQDLFQMKDGREAALLRSAQLDE
                     YAAMIRRFFRRYRTGFDRTVVKRRKS"
     gene            complement(1051..4632)
                     /locus_tag="PLT63_04245"
     CDS             complement(1051..4632)
                     /locus_tag="PLT63_04245"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HOD97969.1"
                     /translation="MPLLPHVMICVFILLLPGEVFGQTATAFSPDDPYFFYNQEKRPD
                     YPGQWHLENNAKAIEGKTNAGVDAGLRGAWKLGYTGKGVVIGVLDDGVEGSHEDIKEN
                     YSKALSRTFLDEQILAPTGPRYLGDNHGTSVAGVAAARGGNGIGGTGAAPYATLADLR
                     VLGDSVSSTDYMKAYLWKSGVEKVDDKLVIKREAEIQVKNHSYHRQDFWTQGDDAVGK
                     VLKQTALNDVIHVWAAGNERGKIDEDSGKNRDLNSSYVLTVGALGSDGKFSDYSSYGA
                     NVFVAAPSNRTDGTGLGIITTDRTGADFGYNRYSKDKNPKGDDGDVFPDMNYVSDFGG
                     TSSATPLVSGIMALGKELNKNMDVRMAKHVLVQTSTRVDPDDNSATGGWVKNGAGNWF
                     NPNYGFGNINAGKFVERLKTVKGVTNQTSAVSGTQTVNKPIEYIDGSGNGGTKQSFTF
                     SATELPDKLRQPLEGVEVNLNFTHSSRGDLTANIVSPYTTKSRLFNATTHFTDPKQQD
                     DKSVTDFSWTFLSNAFWGEDPLGGKDKTSGSWDIIMGDKVKKDSDKLGTWNSYNLTLL
                     MGELIFSDSGTTTQAKDIKARSLSLKSTDESFINPEGKKLEVSERIEVTGGELTANGQ
                     VKMVRPDPDDDGSDEEGGEFILDGGIVSGTGVIEAPYGFYHSQGTIKPGNSIGTLTLN
                     TPDAKGDYYQEPEAKLLIEVASPTSNDLLAVNGAADLQGILETSWTGGYIPAARTKFG
                     NILTASSGVTGRFSRLLTNITPTVLFKPQYDLPNQIYLLVERDYNNENLLPYLTANQR
                     AVGAMLNSVGNTAAGDLDTVLGKLDALGTYRQAAAAMDQIAPRGDMTSVLVTQNDARL
                     QTANISARLQDIRSGVQGVSLRGLNIMIEENDDLNRYGKPVLLAFNGDALPEGFRLEG
                     NDNWGIFATGNGTTGNIKNASAQGDSSFRNVGITAGVDYRFTRNLTVGLMGGYGRTRS
                     DLDNLGSTAVIGTYTLGVYGTYSRKGFYVDGLANYGWSNTDKDRRIVYPGVDRTAASD
                     QRGSTWTFSGGAGYDFTLGNWVLTPKATLDYLHLTTDDYTESGAGALDLRVNGEKTKM
                     LLGQVGGNVTYVWRLDKMTVLPRLWAMYGREFNADEQVGTTARLVMGSAAFTVYAVPP
                     DRDFANIGGGIAAILPKGASLSLNVSGQVGQSNYSAYNLNLGVRIPF"
     gene            complement(5222..6661)
                     /gene="gcvPB"
                     /locus_tag="PLT63_04250"
     CDS             complement(5222..6661)
                     /gene="gcvPB"
                     /locus_tag="PLT63_04250"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003346.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HOD97970.1"
                     /translation="MDLIFEKSRPGRQGCAIPAGDVPAVNPREVIPPSLLRDELPLPE
                     VAEVDLIRHYTALSRRNFGVDVGFYPLGSCTMKYNPKINEDVAKFPGFTGLHPYAPVA
                     FSQGSLQLMYELRQYLSEIFGMADFTLQPAAGAHGELTGIMIFKKYFEKTGEKRTKIL
                     IPDAAHGTNPASGALCGYRTLTVRSNADGGVDIDHLASLMSDDTAGLMLTNPNTLGLF
                     ERNIEKVSDIIHNKGGLLYGDGANANAFLGKVRPGDLGFDVIQLNLHKTFSTPHGCGG
                     PGSGPVGVGKKLVDFLTIPRVIKTDEGFAWSSEYPDAIGRVRTFFGNFNVMVKAYAYI
                     RTLGPAGLKRAAEIAVLNANYIKARLQPYYDLSFDRPCMHECVFSGTRQVRESGVHTS
                     DIAKRLLDYGYHPPTIYFPLIAPEAIMIEPNETESIEILDAFCDAMIQIASEARETPK
                     VVKSAPLTTPVKRLDEVKAAREPNVCWKG"
     gene            complement(6715..7035)
                     /locus_tag="PLT63_04255"
     CDS             complement(6715..7035)
                     /locus_tag="PLT63_04255"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOD97971.1"
                     /translation="MGFYELQDIAGREIVPGFTARFVHSERMTFSYWEVKAGAVLPEH
                     SHPHEQVANLLTGKFEMTIAGETRILASGMVAVIPPEAKHSGRALADCYILDVFYPIR
                     DDYR"
     gene            complement(7275..8339)
                     /locus_tag="PLT63_04260"
     CDS             complement(7275..8339)
                     /locus_tag="PLT63_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97972.1"
                     /translation="MEDKKDAKKLSMASTKQEMLSAYNMLLKQTQEKQEAELKPEKKI
                     EEKKTKEAVQTAESLTAEGVSREINNLKIDIGKMLTQIADRLDTEVNKFAAVQQAIAA
                     KEREIQELYDIEKAAATLAALIEAQNAKRIEFDMEMAKKKEELKEEIDATRAEWRKEK
                     EEYEAAVKEREAADKKKQTRDKEEFDYNFKREQQLAKDRFEYENAKLEKEIKAKKEQA
                     DRELKVREQAVAEREEELTDLRKRAATFPKEMETAVNRAVKEVTDRLTLETKSREEIH
                     KKEYEGERNVLKTRIEALEKIVKEQGDYIAKITQQLEAAYQKVQDIAVKTVEGASDAK
                     SLANLQQFLGDQMRKGGTEK"
     gene            complement(8424..9752)
                     /gene="gcvPA"
                     /locus_tag="PLT63_04265"
     CDS             complement(8424..9752)
                     /gene="gcvPA"
                     /locus_tag="PLT63_04265"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HOD97973.1"
                     /translation="MDYVPHTPADEQRMKEAIGIAVLDDLFTDIPPRFRLRDALALPP
                     GIPELELAALMTDLSARNRLPALTLMGAGSYRHWLPAVVGHVISRSEFYTAYTPYQAE
                     ISQGILQAIYEYQTMIAGLTGMQVANASMYDGATAMAEAAVLAAKTLNRPKLVVARSV
                     HPEYRQVVRTYAWANGYEVVETPYLSTGQADLDVLGRTVDRDTAAVLIQSPNFFGVIE
                     DIAPMAAITHAPGALLVAGFTEATSLGILRPAGALGADFVVGEGQAFGNPLNYGGPYL
                     GIFAAAEKFLRKIPGRLAGATVDKEGKRGFVLTLQTREQHIRRERATSNICTNEALCA
                     LAAAVYLVCLGKNLKELAELNIRNAQYLRARLLELPGFAPAFSGPVYNEFAIRCPDPA
                     AVNRKLQTAGIIGGYELGRDYPDLADGLIFCATELPTKADMDQAINIIRD"
     gene            complement(9890..10288)
                     /gene="gcvH"
                     /locus_tag="PLT63_04270"
     CDS             complement(9890..10288)
                     /gene="gcvH"
                     /locus_tag="PLT63_04270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOD97974.1"
                     /translation="MSKINPTDRRYSREHEWVIDNGNGTATVGITDHAQELLTDIVFV
                     ELPEIGKQVKQMEPVAVVESVKSVSDVYAPVSGEVTEANRKLEQNPELINEDPYGKGW
                     IVRLRMDNPEELPGLMEAADYEAFIAAEEH"
     gene            complement(10333..11427)
                     /gene="gcvT"
                     /locus_tag="PLT63_04275"
     CDS             complement(10333..11427)
                     /gene="gcvT"
                     /locus_tag="PLT63_04275"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOD97975.1"
                     /translation="MKKTPLYNRHVALGARIIDFGGWAMPVQYSTVIEEHLSTRKAAG
                     LFDICHMGEIDVSGPGAVAFLQHVLTRDLTGQKPGQMKLSVLANEAGGIMDDLTVYFF
                     GENRYRLVTNAATKDKDLQWLLGVKAHEGFTEMEIADRTSGIGKLDLQGPQAEEILQV
                     LVPVNLHTLKFYHFLETDVSRVPAVVSRSGYTGEDGFEIYVPSERVGEIWDRLLQVGV
                     DYGLKTVGLGARDTLRLESGMMLYGNDMDETVNPYEVVYGWVVDKEKDFIGAAALREQ
                     FAREVKRKLVGFTMRERGIARHGYKIFKEDREVGVVTSGTYSPTLGKAIGMAFVPSEL
                     AKPGTTFDIQIRNNFAAAQVVPLPFHKRER"
     gene            11715..11788
                     /locus_tag="PLT63_04280"
     tRNA            11715..11788
                     /locus_tag="PLT63_04280"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11747..11749,aa:Gln,seq:ctg)
     gene            11911..12534
                     /locus_tag="PLT63_04285"
     CDS             11911..12534
                     /locus_tag="PLT63_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HOD97976.1"
                     /translation="MKLFPKEVQFSDNFEELAGKIQEGSVLFLEILKDYRNSKGRISQ
                     LKEIEQQADVITHRIYQDLHKTFITPLDREDIYSLANKMDSIMDMIDESASKMNLYRI
                     KEPLPAHIKLAVILNRAIALLNRSIHAMRNRSMNVRTILDACVEINSLENEADHVMRE
                     SMAELFEREKDAIELIKSKELLERIEEATDICEDVSNILEGFILKYG"
     gene            complement(12546..14231)
                     /locus_tag="PLT63_04290"
     CDS             complement(12546..14231)
                     /locus_tag="PLT63_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HOD97977.1"
                     /translation="MSQGANPPPSDFIREIIEEDLKTGKNDGVVAVRFPPEPNGYIHI
                     GHAKSVCLNFGIAAQYGGTCNLRMDDTDPAGESLEFVQSIIDDIHWLGFDWQDRLYYA
                     SDYFERLYQFAVELIKAGKAYVDSMSADEIREHRGSFTEPGRESPCRNRTIAENLDIF
                     TRMRAGEFADGEHVLRAKIDMAAANIVLRDPIMYRIKREPHYRTGTDWVIYPMYDFAH
                     CLSDALEGITYSICTLEFENNRPLYDWFVEQLITGRRPRQIEFARLNVSYTVLSKRRL
                     IELVEKKYVSGWDDPRMPTVAGMRRRGYTPEAIRNFAAAIGVAKNDNLVDMDLLEHCV
                     REDLNERTPRAMAILRPLRVVIDNYPEGRIEAFTAPNHPQKPEMGTRTVPFSRVIYIE
                     RDDFMEEPPKKYHRLGPGREVRLRNAYVIRFERMVKNERTGTIEEVHCTYDPETRSGP
                     PADGRKIPGVIHWVAAAQAVPAEVRLYDRLFRVPEPRDDDYTRDLNPDSLVTLAGCHL
                     EASLRDAAPGARYQFERLGYFCVDTRDSQPGKTVLNRIVPLRDSWAKIAGSGK"
     gene            complement(14289..14864)
                     /gene="def"
                     /locus_tag="PLT63_04295"
     CDS             complement(14289..14864)
                     /gene="def"
                     /locus_tag="PLT63_04295"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF013492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOD97978.1"
                     /translation="MKKVLTMWSDDHVNEEETRILRQPCADLPIPLDGEARKSVKTLV
                     DAFLERNDASGLAAPQIGICKKIIIFRNRGFTRDGASDGTWTKKDVEVLINPRLTQQR
                     GNQVILSEGCLSCPDIKVDIARYPEIKIRGFDMHGQKVSKRYVDFIARIVQHEMDHLE
                     GKLIVDYDEGAIFVPKKKRGFFENLFQNKSA"
     gene            15065..16321
                     /locus_tag="PLT63_04300"
     CDS             15065..16321
                     /locus_tag="PLT63_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase family protein"
                     /protein_id="HOD97979.1"
                     /translation="MQKIVNQTITAIVRREFSRSLRFEDGRPPALPQAREKLPRLLYL
                     HVPFCEKLCPYCSFNRFLFDATRCRRYFHALQREIHLYRDRGYDFAGIYVGGGTPTVM
                     IDELAATLERARECFSIREISVETNPNHLTEDRLNILKSAGVNRLSVGVQSFDDGLLK
                     DVDRLEKYGSGAEVAARLAAIGGRFDTLNADMMFNFPSQTPEILARDLDVLMRIGVDQ
                     VTYYPLMVSDSTRRQVTEKLGEVDYGREEEYYDRILRRLLPTYRLSSAWCFSRQNGMI
                     DEYIVDYDEYAGLGSGSIGFLGGACYANTFDISEYIDKIERGGLPLMAARIFSRKDQL
                     RYDFLMKLFGTRLDLRDLRNKYGLNPWACLWFEILAFLLTGSLRYRNGRLTLTDRGCY
                     SWVIMMREFFIAVNNFRDYCRARLNG"
     gene            16346..17764
                     /gene="selA"
                     /locus_tag="PLT63_04305"
     CDS             16346..17764
                     /gene="selA"
                     /locus_tag="PLT63_04305"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HOD97980.1"
                     /translation="MDDRQRLWLQQLPKIDELMLLLEGQGVADRVPKPLLKELCRATV
                     EKRRQGIIAGSGGKEEPAPSLTGLAGEITRQIDRLHTRRLRRVVNATGVILHTNLGRA
                     PLSPAARECLLEIGCGYSNLEFDLVRGTRGLRYDHIEELLRLLTGAEDALVVNNNAAA
                     VLLALNSLAQGGEAVVSRGELIEIGGEFRIPEVMEKGGVRLREVGTTNRTRLRDYREA
                     VNPETRLLMKIHTSNFKIVGFTEEVPLRDLVALGRECDLPVLNDLGSGCLIDLDRYGR
                     QREPTVEEVVAAGTDVVTFSGDKLLGGPQAGIILGKKTLLERIKRNPLNRALRIDKLT
                     LAALEGTLIAYLNPRQARSDLRVLKALTETRDEVRVRGKKLLRKLRAAGMKGVDFSLR
                     EGTAAAGGGSLPGQEIPTSLVSLTPRSLSSSRLEERLRGLKIPIIARIDGDEILLDMR
                     TVAEEEFVFIVNGLRSIFLQDG"
     gene            17757..18728
                     /locus_tag="PLT63_04310"
     CDS             17757..18728
                     /locus_tag="PLT63_04310"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOD97981.1"
                     /translation="MAENSPPACRFVVGKEEEGLRLDIFLARRQDSLSRSQIPRVLAA
                     GGILLNGKAPKASTRVWRGDEILLAIPPPSSSEVMPEDIPLKIVYEDSSLLVIDKPPG
                     LVVHPGAGNPRGTLVNALLHHCRDLSGIGGVLRPGIVHRLDKDTSGLMIIAKSDSVHQ
                     ALAADFQARRVEKLYLALVCGEMKDDEGIVALPVGRHPVERRKMSTRSPRGKEAVTRW
                     QVRERFGAATLVAAAIETGRTHQIRVHLTSLGYPVVGDRVYGNPKKIMEINDQSLRGR
                     LREMKRQALHASRIAFVHPADGRPLSFSSPLPPDMEDLCRFLRERIA"
     gene            18891..19721
                     /gene="pgeF"
                     /locus_tag="PLT63_04315"
     CDS             18891..19721
                     /gene="pgeF"
                     /locus_tag="PLT63_04315"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HOD97982.1"
                     /translation="MDKPSLNEDKFVWKKNDQVEYFKSPAWEAYSFVAHAFCTRRGGV
                     SSGRFAALNMSGREGEEDANVSANWDIAAAAFGLTRRHFFRIHQVHGDKILVIDSADF
                     LIHTCRPLEYDAVVTNRPGLALCILTADCVPVLVADPARRIVAAVHAGWRGTALNISG
                     QVVTLLRERFGSSTRDIRAAVGPAIGPCCYEVDDAVRAAMDRHPGRDAIFTKQKEPGK
                     WRLDLALANRLQIEAAGVTGGNIQVANLCTSCRRDLFYSHRGEGGKTGRLLNFIMISP
                     "
     gene            19990..21237
                     /gene="rho"
                     /locus_tag="PLT63_04320"
     CDS             19990..21237
                     /gene="rho"
                     /locus_tag="PLT63_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOD97983.1"
                     /translation="MNIEDIKRLPISELANLAKDLNVAGASGMRRQDLIFAILQAQTE
                     KNGLISGTGVLEILPDGFGFLRAVDYNYLPSPDDIYVSPSQIRRFNLRTGDTISGEVR
                     PPKEGEKYFALLKVDTVNHELPDATRDKILFDNLTPLYPEEKLNLELDPDNLSTRIMD
                     LFTPIGKGQRGLIVSPPRAGKTMLLQDIARSIAANHKEVTLMVLLIDERPEEVTDMQR
                     SVQGEVVSSTFDEPATRHVQVAEMVIEKAKRLVEHKKDVVILLDSITRLARAYNTVVP
                     PSGKVLSGGVDSNALHKPKRFFGAARNIENGGSLTIISTALIDTGSRMDEVIFEEFKG
                     TGNMELHLDRRIADRRIFPAFDLIRSGTRKEELLIPKNNLNRIWILRRLLQEMNPVDA
                     MDFIIDKIRKAENNQLFLDSMNQ"
     gene            21325..21543
                     /gene="rpmE"
                     /locus_tag="PLT63_04325"
     CDS             21325..21543
                     /gene="rpmE"
                     /locus_tag="PLT63_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012439895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOD97984.1"
                     /translation="MKEGIHPPYYETTITCVCGNVISTRSTKKDIKVEICSQCHPFMT
                     GKQKIIDTAGRVERFHKKYAGVGKKVGK"
     gene            21960..>22585
                     /locus_tag="PLT63_04330"
     CDS             21960..>22585
                     /locus_tag="PLT63_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HOD97985.1"
                     /translation="MYSRLKEIVRRYEELQGLLSDGEIVAKPSLYQKYAKEHADLHDL
                     VEVYRAYEEVRGRIEEGQFLLKENDEELKEIVREEMPLLKAQLEELDGHLKVLLLPRD
                     PNDDKNVLLEIRAGTGGDEAGLFAADLYRMYGRYAERSGWKMEVISNSPAGGVGGFKE
                     IIALIEGRGAYSRLKYESGVHRVQRVPVTETQGRIHTSAVTVAILPEA"
BASE COUNT         4519 a         6304 c         6577 g         5185 t
ORIGIN      
        1 tgcttttccg gcgcttgacc acggtgcggt cgaaccccgt gcggtagcgg cggaaaaatc
       61 gtcggatcat cgcggcgtac tcgtcgagtt gggcggaacg gagtagggcc gcctcccggc
      121 cgtccttcat ctggaagagg tcttggctct tggaatgttt tcccgacttg cccgtgatgc
      181 cccggaagaa ggcgagatat tcgcagagaa aggcctcact cgccaggggc agggccgtat
      241 cggggtcatg ggccaggtcg tagagaagct gcagaggaga ggcatgggtg aggtgttcgt
      301 tttccgtgcg aacggtgttg gaaaagaccc ggatgcactc ccgggcgttg cgtttttccg
      361 tgatgttcat atctgaataa taggtgtcgg gcttcatgtt gaacatggag cggttcaggt
      421 agttgaagta gtcgaaaagc agatggcgtg cctcctcgat gtgcttggaa tccctgagga
      481 tcttcttgat cttggggtcc gcctgccaga gtcgttccag cagttcctct ttggagagga
      541 aaataacgtt cttcatcatt ttttcacctg attttggaaa tggcttgggc cgattctttt
      601 ttgttttttc atcatgattt caacatttct atggacatac cgcttccggg ctgatccgtt
      661 aaatccttgg ggtatgaaga ctgcctctat ttgtcgatat tgtcaacctt cgttttcatt
      721 catatgcaat ccctgatgca agttcctgct gcggcaaagg cgggtgggtc ctgggagacg
      781 ggattttgct gtctgaagtt gatcccacct attccgatga tctcaaggag tcaggagatt
      841 gtgttgaaaa ggcatttctg agaatagctg ccatactatg agtgcgaggc ccggatgcgc
      901 cataagaacg gcgactggat ctgggccctc gatagcgggg aagccggcgg cctggacgga
      961 tgaccggaaa ctgttggtct tgtccggcat gcattttggt caaggcattg acaaggaaaa
     1021 atcatcccat ccacatatgt ccgccggttc tcagaagggg attctcacgc cgaggttgag
     1081 gttataggcg gagtagttgc tctgtcccac ctggccggag acgttcaagg agagggacgc
     1141 tcccttggga agaatcgccg cgatgccgcc gccgatgttg gcgaagtccc ggtccggcgg
     1201 gacggcatat acggtaaagg cggcgctgcc catcaccagc ctggccgtgg ttcccacctg
     1261 ctcatcggcg ttgaattccc ggccatacat cgcccacagg cggggcaaaa cggtcatttt
     1321 gtccaatctc cagacatagg tcacattgcc gccgacctgt cccagaagca tctttgtctt
     1381 ttcgccgttt acccgcagat cgagggcacc cgccccggac tccgtataat catccgttgt
     1441 caagtgaaga taatccaggg tcgccttggg ggtaaggacc cagttgccga gggtgaagtc
     1501 atagccggca ccgccgctga aagtccaggt ggatccccgc tggtccgagg cagcggtgcg
     1561 gtcaactccg ggataaacga tgcgacggtc cttgtcggtg ttgctccagc cgtaattggc
     1621 caaaccgtcc acataaaagc ccttgcggct atatgttccg taaacgccca gggtgtatgt
     1681 gccgatcacg gcggtgctgc cgagattatc gagatcgctc ctcgttctgc cgtacccccc
     1741 catgaggccg acggtcagat tccttgtgaa gcggtagtca acaccggcgg taatgcccac
     1801 attccgaaag gaactgtctc cctgggcgga ggcattcttg atgtttcccg tcgtgccgtt
     1861 ccccgtggcg aatatccccc agttgtcgtt gccttccaac cggaatccct cgggcagggc
     1921 gtcgccgttg aaggccaaca agacgggctt cccataacga ttcaaatcgt cgttctcttc
     1981 gatcatgatg ttcagccccc gcagactcac accctgaacg ccgctgcgga tatcctggag
     2041 ccgggcggag atgttggccg tctgaaggcg ggcatcattc tgggtcacca acaccgatgt
     2101 catgtctccc cggggggcga tctgatccat ggcggcggcg gcctgcctgt aagtgcccag
     2161 ggcgtcgagc tttcccaaaa ccgtatcgag atcacccgcg gccgtgttgc ccacggaatt
     2221 gagcatggcg cccacggccc gctgattggc cgtaagatag ggcaggaggt tctcgttgtt
     2281 gtaatcccgt tccaccagga gataaatctg gttggggagg tcgtactggg gcttgaagag
     2341 gaccgtgggg gttatattcg tgaggaggcg ggaaaagcgg cccgtcactc ccgaggaggc
     2401 ggtaaggata ttgccgaatt tggtccgcgc ggcggggata tatccccccg tccaggaggt
     2461 ttcgaggatg ccctggagat cggcggcacc gttgacggcc agcaggtcgt tgctggtcgg
     2521 ggaggctacc tcgataagga gtttggcctc gggttcctgg taatagtctc ccttagcgtc
     2581 aggggtgttc agggtgaggg tgccgatgga attgcccggc ttgatcgtgc cttgcgagtg
     2641 gtaaaaaccg tagggggcct cgataacccc tgtccccgag actatgccgc catcgaggat
     2701 aaactccccc ccttcttcgt cgctgccgtc gtcatccgga tcgggccgga ccatttttac
     2761 ctgcccgttg gcggtcaact ccccgccggt cacctcgatc ctctcgctga cttcgagctt
     2821 tttgccttcc ggattgatga agctctcatc ggtgcttttc aaggacaggg acctggcctt
     2881 gatgtccttg gcctgcgttg tcgtgcccga gtcgctgaag atcagctccc ccatcaacag
     2941 ggtaaggttg taactgttcc atgtccccag cttgtcactg tcctttttta ccttgtcccc
     3001 catgatgata tcccaactgc cgctggtttt gtcctttcca ccgagggggt cctctcccca
     3061 aaaggcgtta ctgagaaaag tccaggaaaa atcggtgaca cttttgtcgt cttgctgctt
     3121 tggatcggta aagtgggtag tggcgttgaa gaggcggctt ttcgtcgtat atggggagac
     3181 aatgtttgcc gtcaggtcgc cccggcttga atgggtgaag ttgagattca cctccacccc
     3241 ctcgaggggc tgtcgcagct tgtcaggcag ttccgttgcc gaaaaagtga aggactgctt
     3301 cgtcccgcca ttgccgctgc cgtcgatata ttcgatcggc ttgttgacgg tctgagtccc
     3361 tgatacggct gaggtctggt tcgtgacccc cttgacggtc tttagcctct ccacgaactt
     3421 gccggcgttg atgttgccga aaccgtaatt gggattgaac caattccccg ccccgttttt
     3481 cacccagccg cccgtggcgc tgttatcgtc ggggtcaacc cgggtactgg tttggaccag
     3541 gacgtgcttc gccatgcgga catccatatt tttgttcaat tccttgccca gggccataat
     3601 gccggagacc aggggcgtgg cggaagaagt tcccccgaaa tcgctgacat aattcatatc
     3661 gggaaacaca tctccatcat cgcccttggg attcttgtcc ttggaatagc ggttgtaacc
     3721 gaaatcggcc cccgttctat ccgtggttat gatccccagc ccggtgccgt cggtccggtt
     3781 cgagggggcg gcaacaaaga cattggcgcc ataactgctg taatcggaaa atttcccgtc
     3841 gctccccagg gcgcccacgg taaggacata actgctgttg agatccctgt ttttccccga
     3901 atcttcatct atctttcccc gttcattgcc cgccgcccag acatgaatga cgtcgttcaa
     3961 tgccgtctgc ttgagcacct tcccgacggc gtcgtcgcct tgcgtccaaa agtcttgccg
     4021 gtggtagctg tgattcttga cctggatctc cgcctccctc ttgatcacca gtttgtcgtc
     4081 caccttctct acgccgcttt tccacaggta ggccttcata tagtccgtcg aactgacgct
     4141 gtcgcctaaa acccggagat ccgcgagggt ggcatacggg gcggcaccgg tgccgccgat
     4201 gccgttgccg ccccgggcgg cggccacgcc cgccacggat gtcccgtggt tgtccccgag
     4261 gtatcttggc cccgtgggag ctaggatctg ctcgtccaga aaggtgcggc tcaacgcctt
     4321 gctgtaattt tccttgatgt cctcgtggct tccctccacg ccgtcgtcca ggacgccgat
     4381 gaccaccccc ttgcccgtgt atccgagctt ccacgccccc cggagcccgg catcgacgcc
     4441 ggcgtttgtc tttccctcga tggctttggc attattctcc agatgccact ggcccggata
     4501 atccggtctt ttttcctgat tgtagaaaaa ataaggatca tcgggactaa aagccgtggc
     4561 ggtttgaccg aaaacctcgc cgggtaataa aaggataaag acgcagatca tgacgtgcgg
     4621 cagtaagggc attcctaaaa aaatcatgct tttcatagac ttgaggtttt tatttcgcat
     4681 gagagaacat tctttatgag ttcactttat cggggattcc agtcaccgag ttgccattat
     4741 ttacattcca cgaattttta ttcccctatg ggatagggag aaaagtcctc gccttgaggc
     4801 taaggctata gtctgattgc ggtatttatc gctatatgat gtcgatgcat accgtttaag
     4861 acatcaaatc tcaccttcgt tagaccacaa aatgatacaa gccggtttcg tgagctacca
     4921 ttgcccagat agttggggat tgatccgtga gacttgccgg acgaatagtg gagaaatcat
     4981 tatgtgccat atatcttgat tatatagata tttttgtgtt tgtgatgccg catgcttgct
     5041 tcaatcacct ggttcggctg acccagggga agacgtctca taaagatgat gatggaattc
     5101 agagtatgag tcgcgcattt tgggaatgtc acaaatgagc ttggagaatt tagtggccag
     5161 ttccggtata acgtgaccta tgaagtaatc atgctatgcg aactatgagc tttcatgagg
     5221 tttacccttt ccagcaaaca ttcggttccc gggccgcctt cacctcgtcg aggcgcttca
     5281 cgggggtggt caggggggcg cttttaacta ctttgggggt ttcccgcgcc tcggaggcga
     5341 tttggatcat ggcgtcgcag aaggcgtcca ggatttcgat gctttccgtt tcgttgggct
     5401 cgatcataat tgcctcgggg gcaatgaggg ggaagtagat cgtcgggggg tggtagccgt
     5461 agtcaaggag gcgcttggcg atatccgagg tatggacccc gctttccctg acctggcggg
     5521 tgccggaaaa tacgcactcg tgcatgcagg gccggtcgaa agaaagatcg taatagggtt
     5581 gcaggcgcgc cttgatgtaa ttggcgttca ggacggcgat ttcagcggcc cgcttcagcc
     5641 ccgccggccc cagggtgcgg atataggcat aggccttgac catgacgttg aagttgccga
     5701 agaaggtccg gacccggccg atggcgtcgg ggtattcgga ggaccaggca aagccttcat
     5761 cggtcttgat cacccgggga atagtcagga aatccacgag ttttttaccc acacccacgg
     5821 gaccgctccc cggaccaccg cagccgtgag gggtggaaaa tgtcttgtgc agattcaact
     5881 gaatgacgtc gaagcccaga tctccggggc ggaccttgcc gaggaaggcg ttggcgttgg
     5941 cgccgtcacc atagaggagc ccgcctttat tgtgaatgat atcggatact ttctctatgt
     6001 tccgctcgaa aagccccagg gtgttgggat tggtcagcat gaggccagcc gtatcatcgg
     6061 acatgaggga ggcgagatga tcgatgtcca cgccgccgtc ggcattggag cgtaccgtca
     6121 aggtccggta gccgcagagg gcccccgagg cgggattggt gccgtgggcg gcatcgggga
     6181 tgaggatttt cgtccttttc tcccccgtct tctcgaagta ttttttaaag atcatgatgc
     6241 cggtaagctc cccgtgggcg ccggcggcgg gctggagggt gaaatccgcc atcccgaaga
     6301 tctcgctcag atattgtctc aattcataca tgagttggag gctgccctgg gaaaaggcga
     6361 cgggggcgta aggatgcagg ccggtgaagc cggggaactt ggccacgtcc tcgttgatct
     6421 tggggttgta tttcatcgtg caggagccta gggggtagaa acccacatcg acgccgaagt
     6481 tgcgccttga gagggccgta tagtggcgga tcagatccac ctcggcgacc tccggcaggg
     6541 gcagctcgtc ccggagcagg gacggaggaa tgacctcccg cggatttacg gcgggtacgt
     6601 ccccggcggg aatggcacaa ccctgtcgtc cgggacgact cttctcaaat atcaaatcca
     6661 tcttttatcc ctccttctcc agcccagccg cggtcctcat tttcagctgc catttcagcg
     6721 gtagtcatcg cggatcggat agaacacatc caagatgtag caatccgcca gggcccggcc
     6781 gctgtgcttg gcctccgggg ggatcaccgc caccatgccc gacgcgagaa tgcgggtctc
     6841 gccggcaatc gtcatttcga acttgccggt aaggaggttg gccacctgct cgtgggggtg
     6901 gctgtgttcc gggagaaccg ccccggcctt gacctcccag taggaaaaag tcatgcgctc
     6961 ggaatgaacg aaccgggcgg tgaaccccgg gacgatttcc cggccggcga tatcctgcaa
     7021 ttcataaaat cccattgtct tcgcctccct gtttatgtga aaaactcctc aatatgccca
     7081 ttttcatttt tgtgataaaa cgggacatgg ctgttaaagc gttgttgcat ctttatgtcc
     7141 gcatagcttt ctgtaaatag ttaaaaaaat caaaaaataa aatttggtta tgtttaacgt
     7201 gaatgtgtcg tcagttatcg gggatgcccg tcaatgcgat gatcctattt ctaaagtcct
     7261 cgtattcgtt gcccttattt ttccgtcccg cccttgcgca tctgatcgcc caggaactgt
     7321 tgaaggttgg ccagggactt tgcgtccgag gccccctcca cggtcttcac ggcgatgtcc
     7381 tggaccttct gataggccgc ttccagttgc tgggttatct tggcgatgta atcgccctgc
     7441 tccttgacga tcttctccaa cgcttcgatc ctggttttca ggacgttccg ctccccttcg
     7501 tattctttct tatggatttc ctcccggctt ttcgtttcca gcgtcaaccg gtccgtgacc
     7561 tcttttacgg ccctgttaac cgccgtttcc atctccttgg ggaaggtggc ggcccgtttt
     7621 ctcaggtccg tcaactcctc ctcgcgctcc gcgacggcct gctcccggac cttcaattcc
     7681 cggtcggcct gttccttttt tgccttgatt tccttttcca gcttggcgtt ttcatactcg
     7741 aaacgatctt tggccaactg ttgttcccgc ttgaagttat agtcgaactc ctccttgtcc
     7801 cgggtctgtt tctttttatc ggcggcttcc cgctctttga cggcggcctc gtactcttcc
     7861 ttttctttcc gccattccgc acgggtggcg tcgatttcct ccttcaattc ttcctttttc
     7921 tttgccattt ccatatcgaa ctcaatacgt ttggcgtttt gcgcctcaat gagggccgcg
     7981 agggttgccg ccgccttttc tatgtcatag agttcctgga tttcccgttc cttggcggcg
     8041 attgcctgct ggacagcggc aaatttgttg acttccgtat cgaggcgatc ggcgatctgg
     8101 gtaagcattt tgccaatgtc gatcttcaga ttgttgattt cccgggaaac gccttccgcc
     8161 gtgagagatt cggcggtctg tacggcttct ttcgtttttt tctcctcgat ctttttttcc
     8221 ggcttcagtt ccgcctcctg tttttcctga gtttgtttta gcaacatgtt gtaggccgac
     8281 agcatttcct gtttggtgct ggccatggag agtttttttg catctttttt gtcttccata
     8341 attgatcctt ccttcctttt gctgcattat gattgctgtc tgatgaaccg gatcggcagc
     8401 ttggaaccat taaatggttc tcgttaatct cttatgatat taattgcttg atccatgtct
     8461 gctttggttg gcagctctgt ggcgcagaag atgagtccgt ccgccaggtc cgggtagtcc
     8521 cggcccagtt catagccgcc gatgatgccg gcggtctgga gcttcctgtt gacggcggcc
     8581 ggatcagggc agcggatggc gaattcgttg taaaccggtc cggagaaggc cggggcgaag
     8641 ccgggtaact ccaggagtcg ggcgcgcaga tattgggcgt tcctgatatt caactccgcc
     8701 agttccttga ggtttttccc caggcagacc agatagacag cggcggcgag ggcgcagagg
     8761 gcctcattgg tacagatgtt ggaggtggcc cgttcccggc ggatgtgctg ctcccgggtc
     8821 tggagggtga ggacgaagcc ccgcttccct tccttatcca ccgtagcccc ggcgagacgt
     8881 ccggggatct tgcggagaaa cttttccgcg gcagcgaaga tccccaggta gggtccgccg
     8941 taattgaggg gattgccgaa ggcctgacct tcgccgacga cgaaatccgc ccccagggcg
     9001 ccggcgggcc tgaggattcc cagggaggtc gcctcggtga agcccgccac cagtaaggcc
     9061 cccggcgcgt gggtgatggc cgccatgggg gctatatcct cgatgacgcc gaagaaattg
     9121 gggctctgga tcaggacggc ggctgtgtcc cggtccacgg ttcggcccag gacgtcgaga
     9181 tccgcctgac ccgtggacag gtacggcgtt tccacaacct cataaccgtt ggcccaggcg
     9241 taggtccgga cgacctgccg gtattcggga tggacggatc ttgccacgac cagctttggc
     9301 cggttcagcg tcttggcggc gaggacggcg gcctccgcca tcgccgtggc cccgtcgtac
     9361 atggaggcgt tggcgacctg catccccgtc agcccggcga tcatggtctg gtattcgtag
     9421 atcgcttgga ggataccctg gctgatctcc gcctggtaag gggtgtaggc ggtatagaat
     9481 tccgaacggg aaatgacatg gcccacgacg gcggggagcc agtgccggta ggacccggcc
     9541 cccatgaggg tgagggcggg gaggcggttc cgcgcgctga ggtccgtcat gagcgccgcc
     9601 aattccagtt ccggaatgcc ggggggaagg gccagggcgt ccctgaggcg gaagcggggg
     9661 gggatatccg taaacaaatc gtcgagaacg gcgatgccga ttgcctcttt catccgttgc
     9721 tcgtccgccg gggtgtgggg aacgtaatcc atcgttttat cccttatggt tttgtgtggt
     9781 taatactcct cttctcaccc tataaaaccg ggtgacatgg aagcaagcgg tcgtcccggc
     9841 aggcttcccc accgctcaag caagggcgcc aggaacttgg gcgtctatgt caatgttcct
     9901 ccgcggcgat gaatgcctcg tagtccgccg cctccataag ccccggcaac tcctccggat
     9961 tgtccatcct cagccggaca atccagccct tgccgtaggg atcctcgttt atcaactcgg
    10021 ggttctgctc cagtttcctg tttgcctcag tgacttctcc gctcaccggt gcgtaaacat
    10081 cggagaccga cttgacggat tccacgacgg ccactggttc catctgtttg acctgtttgc
    10141 ctatctccgg cagctccaca aacacgatat ccgtgagcag ctcctgggca tgatcggtga
    10201 tgccgacggt ggccgtgccg ttgccgttgt cgatgaccca ttcatgctcc cgggagtatc
    10261 tcctgtccgt gggattgatc tttgacataa ccttctttct tcctttcaga taaagatatt
    10321 gaaaaagacg tgctaacgct ctcttttatg gaacggcaag gggactacct gggcggcggc
    10381 gaagttgttc cggatctgga tgtcgaaggt ggtccccggt ttggccagct ccgacgggac
    10441 gaaggccatc ccgatggcct ttccaagagt gggggaatag gtgccggagg tgacgacgcc
    10501 gacctcccgg tcctccttga agatcttgta accatggcgg gcgatgcccc gttccctcat
    10561 ggtaaatccc accagttttc tctttacctc ccgggcgaac tgttcccgga gggccgccgc
    10621 gccaatgaaa tctttttcct tgtccacgac ccacccataa accacttcat agggattgac
    10681 cgtctcatcc atgtcgttgc cgtagagcat catccccgat tcgagacgca gggtgtcccg
    10741 ggcccccagg ccgacggtct tcaagccgta atccaccccc acctggagga ggcggtccca
    10801 tatttccccc acccgttcgc tggggacata gatttcaaag ccgtcctcac cggtgtagcc
    10861 gcttcgggac accacggcag ggacgcggga cacatccgtt tccaggaagt gatagaattt
    10921 aagggtgtgg agattgacgg gcacgaggac ttggaggatc tcctcggcct gaggtccctg
    10981 gaggtccagc ttgccgatgc cggacgttcg gtccgcgatt tccatttcgg tgaaaccctc
    11041 atgcgccttg accccgagga gccactggag atccttgtcc ttggtggcgg cgttggtgac
    11101 gaggcggtag cggttctctc caaaaaaata gacggtgaga tcgtccatga tgcctcccgc
    11161 ctcgttggcc agaacgctca acttcatctg accgggtttc tgccccgtca ggtccctggt
    11221 caagacgtgc tgcaagaaag cgacggcgcc cggtccagag acgtcgatct cacccatatg
    11281 gcagatgtcg aatagtcccg cagccttgcg ggtcgacaga tgctcttcga taacagtgga
    11341 gtactgcacc ggcatggccc agccgccgaa gtcgatgatg cgggctccca gggcgacatg
    11401 gcgattgtaa aggggtgttt tcttcatgga aacctcttaa aactgcaatg tcgattttct
    11461 tcaatagcaa tggttagggc taatggtttt acgacgacgc cattggtcaa agacttgggg
    11521 atatgggtac tatatcgcga ggggattttc aaggttaaaa tatgactgtt gcctttcatc
    11581 ctcctggtgc ggcaaatacc ttcggaaaaa aagaagttga cagaacataa tgatcgcact
    11641 atattcccga ccgctcttga agatagaggc aacaaggcct gtccaggtgg tggagaaagc
    11701 attgatgtcg attctgggag atcgttcaac ggcaggacaa cggactctga ctccgtgaat
    11761 cgtggttcga atccacgtct cccagccaaa ataatatcga ggggttagcc aggtaaaggt
    11821 tgaccccttt tgatttttgc ccataattga cggggcgaac cattttgtct ataatccatc
    11881 cgtgaacagt gagggagggg ggcaacggcc atgaagcttt ttcccaagga agtgcagttt
    11941 tccgataact tcgaggaact ggcgggcaag atccaggaag gaagcgtcct ttttttagag
    12001 atattgaaag actaccggaa ttcgaaagga aggatatccc agctcaaaga gatcgaacag
    12061 caggcggacg tgatcaccca tcggatctac caggatctcc ataaaacctt catcaccccc
    12121 ttggaccggg aagacatcta ttccctggcc aataagatgg acagcatcat ggacatgatc
    12181 gatgaatcag ccagcaagat gaatctgtac cggatcaagg aaccccttcc cgcccatata
    12241 aaactcgcgg tcatcctcaa cagggccatt gccctcctga atagatcgat tcatgccatg
    12301 agaaaccgca gcatgaacgt ccggacaatc ctggatgcct gcgtggagat caattccctg
    12361 gaaaacgagg ctgatcatgt aatgcgggag tccatggcgg agcttttcga acgggaaaaa
    12421 gacgccatcg aattgatcaa gagtaaagaa cttctcgaaa gaattgaaga agctaccgat
    12481 atctgcgagg atgtatccaa catccttgag ggattcatcc tgaaatacgg ataaccggtc
    12541 gttacttatt tgccgctgcc ggcgatcttg gcccaggagt cccggagggg gacgatacgg
    12601 ttcagaaccg ttttccccgg ttgcgagtct cgggtgtcca cgcagaaata accgagacgt
    12661 tcgaactgat accgcgcccc cggtgccgcg tccctgaggc ttgcctccag atggcagccg
    12721 gcaagggtca ccagggaatc gggattcaaa tcccgggtgt aatcatcgtc ccgcggttcg
    12781 gggacccgga agaggcggtc gtaaagccgc acctccgccg ggacggcctg cgccgccgcc
    12841 acccagtgga tgaccccggg gatcttcctg ccgtccgccg gggggccgct ccgggtctcc
    12901 gggtcgtaag tgcagtggac ctcttctatg gtgccggtcc gttcattttt taccatccgt
    12961 tcgaaacgga tcacataggc gttccggagc cgaacctcac gaccgggacc gagacggtgg
    13021 tacttcttcg gcggctcttc cataaaatcg tcgcgttcga tatagatgac ccgggagaag
    13081 ggcacggtgc gcgttcccat ctccggcttt tgggggtggt tgggggcggt gaacgcttca
    13141 atccgtcctt ccggatagtt gtcgatgacc acccggaggg ggcgcaggat cgccatggct
    13201 cgaggcgtcc gttcgttcag gtcctcccgg acgcaatgtt ccagcagatc catgtccacg
    13261 agattgtcgt ttttggccac gccgatggcg gcggcgaagt tcctgatcgc ctccggtgta
    13321 taaccccggc gtcgcatgcc ggccaccgtg ggcatccggg ggtcgtccca gccggagacg
    13381 tatttctttt ccaccagttc gatgaggcgg cgcttgctca ggacggtgta actgacgttg
    13441 agccgggcga actcaatctg ccggggccgt cgtcccgtta tcagttgttc cacgaaccag
    13501 tcatatagcg ggcggttgtt ctcgaattcc agggtgcaga tggaataggt gatcccctcc
    13561 agggcatcgg agagacagtg ggcgaaatcg tacatggggt agatcaccca atcggtgccg
    13621 gtccggtagt ggggttccct cttgatgcgg tacatgatgg ggtcccgcaa gacgatattg
    13681 gcggcggcca tgtcgatctt ggcccggagg acgtgttccc cgtcggcgaa ttccccggcc
    13741 cgcatccgtg tgaagatgtc cagattttct gctatcgtgc ggttgcggca ggggctttcc
    13801 cggcccggtt ccgtgaagct gccccggtgt tcacggatct cgtcggcgct catgctgtcc
    13861 acgtaagcct tgccggcctt gattaattcc acggcgaact ggtatagccg ttcgaagtaa
    13921 tcagaggcgt aatagagtcg gtcctgccag tcgaagccca gccagtggat gtcgtcgatg
    13981 atggactgga caaactccag ggattctccg gcgggatccg tgtcgtccat gcggagattg
    14041 caggtgccgc cgtactgggc ggcaatgccg aagttaaggc atacggactt ggcgtggccg
    14101 atatggatat atccgtttgg ctcgggcgga aaacggacag ccacaacgcc gtcgttttta
    14161 ccggttttga ggtcctcctc gatgatttcg cggatgaaat cgctgggggg cgggttcgct
    14221 ccttggctca tgattttata actcctgagg atgtattatg gaaataaatc atctctttag
    14281 atctgatttc aggcagattt attctgaaag agattttcga agaaaccgcg tttctttttg
    14341 gggacgaaga tcgccccttc gtcgtaatcg acgatcagtt tgccctcgag gtgatccatc
    14401 tcgtgctgga cgatacgggc gatgaaatcg acatagcgtt tgcttacctt ctggccgtgc
    14461 atatcgaagc cccggatctt gatctccgga tagcgggcga tgtccacctt gatgtcggga
    14521 caggacaggc agccctcgga gaggataacc tggttgcccc gttgctgggt caggcggggg
    14581 ttgatgagca cctccacatc ctttttcgtc caggtgccgt cggaggcccc gtccctggta
    14641 aagccccggt tgcggaagat aatgattttc ttgcagatac cgatttgtgg agccgccagt
    14701 cccgaggcgt cgttcctctc caggaacgca tcgacgaggg tctttacgga tttccgggcc
    14761 tcgccgtcca gggggatggg gagatccgcg cagggttgtc ttaaaatgcg agtttcttct
    14821 tcatttacat ggtcgtcgga ccacatggtc aggacttttt tcatggccgt gccgcgcctc
    14881 tcataataaa taatatatgt tgccgcctgc cgcgttcgct catcgtgagg ggagtgggaa
    14941 agggtaatta atatggacgg ctgggctgcc cacctatacc accgggaggt tatttatgca
    15001 agacgaaccg cgacggcgaa taatcttgaa caaatgccgc cgatttgata ggtgactcga
    15061 cgatatgcaa aagattgtca atcagaccat taccgccatc gtccgccggg agttctcccg
    15121 ctccctgcgc ttcgaagacg gtcgcccgcc ggcgctgccc caggcccggg aaaagctgcc
    15181 ccgcctgctt tacctccacg tccccttttg tgaaaaactc tgtccttact gttccttcaa
    15241 tcgattcctc ttcgatgcca ctcggtgccg gcgctacttt catgccctac agcgggagat
    15301 ccatctctac cgggaccggg gttatgattt tgccggcatt tatgtcggcg gtgggacgcc
    15361 gacggtgatg atcgatgaat tggccgccac cctggagagg gcgcgggagt gcttttccat
    15421 tcgggaaata tcggtggaga ccaatcccaa ccacctgacg gaagaccgcc tcaacattct
    15481 aaagtcggcg ggggtgaacc gcctgtccgt aggggtccag agcttcgacg acggtctcct
    15541 gaaggatgtg gaccgcctgg aaaaatacgg ttccggcgcg gaggtcgccg cccgcctggc
    15601 ggcgatcggc ggccgtttcg acaccctcaa tgccgatatg atgttcaatt ttccttccca
    15661 gaccccagag atcctggccc gggacctaga tgtcctgatg aggattggcg tcgatcaggt
    15721 gacctattat cccctcatgg tttccgactc gacgcgacgg caggtgacgg agaagctggg
    15781 cgaggtagat tacggccgag aagaggaata ctacgaccgg atccttcggc gcctgctccc
    15841 tacctaccgg ctttcctccg cgtggtgctt ttcccggcag aatggcatga tcgacgagta
    15901 tatagttgat tacgacgaat atgccggcct gggcagcggt tccatcggct ttctgggtgg
    15961 cgcctgttat gccaatacat tcgacatatc tgaatacatt gataaaattg aaagaggggg
    16021 attgccgctc atggcggcca gaatcttcag ccggaaggat cagctccgct atgattttct
    16081 gatgaaactc tttgggacgc ggctggactt gagggatttg cggaacaaat acggactgaa
    16141 cccctgggct tgcctctggt tcgagatcct ggcgttcctc ctgacgggaa gcctgcgcta
    16201 tcggaacgga cggctgaccc tgacggatcg gggatgctac agttgggtga tcatgatgcg
    16261 ggagtttttc atcgccgtaa acaatttccg cgactattgc cgggcccgtc tgaacggctg
    16321 accggtactg cttaaggagg agaccatgga tgaccggcag cggttgtggc tgcaacagct
    16381 tcccaagatc gacgaactga tgctcctgct cgagggccag ggggttgcgg accgggtgcc
    16441 caagcctctc ctcaaggagc tttgccgcgc cacggtggaa aagcgccgcc agggtatcat
    16501 cgccgggagc gggggaaaag aggaacccgc tccttccctg acggggctcg ccggggaaat
    16561 aaccaggcaa atagaccgtc tgcacacccg ccgcctgcgg cgggtggtca acgccaccgg
    16621 ggtcattctc cacaccaacc tgggccgggc gcccctgtcc ccggcggccc gggaatgcct
    16681 cctcgagatc ggttgcggct actccaatct cgagttcgat ctggtaagag ggactcgggg
    16741 tttgcgatac gatcacatcg aggaactcct ccgcctcctc accggggctg aggacgcctt
    16801 ggtggtcaac aacaacgcgg cggcggtcct gctggccctc aattccctgg cccagggtgg
    16861 ggaagccgtc gtatcccggg gggaacttat tgaaatcggt ggtgaattcc gtatccccga
    16921 agtcatggag aagggcggtg tccgcctccg cgaggtgggt accacgaacc gtacccgcct
    16981 ccgggattac cgggaggccg tcaacccgga aacccgcctg ctcatgaaga tccacacgag
    17041 caatttcaag atcgtcggct ttacggagga ggtgccgctc cgggaccttg tggccctggg
    17101 ccgggaatgt gacctccccg ttctgaacga tttgggcagc ggctgcctca tcgacctgga
    17161 ccgctacggc cgacagcggg agccgacggt ggaagaggtg gtcgcggcgg ggacggatgt
    17221 cgtgaccttc agcggcgaca agcttctggg gggtccccag gcggggatca tcctggggaa
    17281 aaagaccctg ctggagcgga taaaaaggaa tcccctgaac cgggccctgc gcatcgacaa
    17341 gctgaccctc gccgccttgg agggaacgct gatcgcttac ctgaacccgc ggcaggcccg
    17401 aagcgatctg cgggtcctca aggcccttac ggagacccgg gacgaggttc gggtgcgagg
    17461 gaaaaagctc ctccggaagc tcagggcggc cgggatgaaa ggtgtcgatt tctcgcttcg
    17521 ggagggaacc gcggcggcgg gagggggctc tctccccggc caggaaatcc ccacctccct
    17581 cgtttccctg acaccccggt ccctgtcttc ctcccgcctg gaggagcgcc tcaggggtct
    17641 gaagattccc attatcgccc ggatcgacgg ggatgagatc ctgctcgata tgcgcaccgt
    17701 ggccgaggag gaattcgttt tcatagtcaa cggattgcgg agtatttttt tacaggatgg
    17761 ctgagaattc cccgcccgcc tgccgctttg tcgtgggtaa agaagaagag ggcctacgcc
    17821 tcgacatctt cctggcccgc cggcaggatt ccctctcccg ctcccagatc cccagggtcc
    17881 tggcggcggg gggcatcctt ctcaacggca aggcgcccaa ggcatccacc cgggtgtgga
    17941 ggggggacga aatccttctg gcgattcccc cgccgtcttc gagcgaggtc atgccggagg
    18001 acatccccct gaagatcgtc tatgaggatt cctccctgct ggtgatcgac aagccgccgg
    18061 ggctggtcgt ccatcccggg gccggaaatc cccggggaac cctggtcaac gccctgctcc
    18121 atcactgccg ggacctttcg gggatcggcg gcgtgctgcg ccccggcatc gtccatcgtc
    18181 tggacaagga cacttcggga ttgatgatta tcgccaaatc cgattcggtc caccaggcgc
    18241 tggcggcgga ttttcaggcc cgacgggtgg aaaagctcta cctggccctg gtgtgcgggg
    18301 agatgaaaga tgacgagggg atcgtcgctc tcccggtggg tcgccatccc gtggagcgcc
    18361 ggaagatgtc cacccggagc ccccggggga aagaggccgt gacccgctgg caggttcggg
    18421 aacggttcgg agcggccacc ctggtggccg cggccatcga aacggggcgt acgcatcaga
    18481 tccgggtgca tctcacatcc ctggggtatc ccgtcgtcgg cgaccgcgtg tacggtaatc
    18541 ccaagaaaat aatggagata aatgatcagt cgctacgagg gcgcctcagg gagatgaagc
    18601 gacaggccct ccatgccagc cggatcgcct tcgtgcatcc cgccgatggc cgaccactgt
    18661 cgttttcatc gccccttccg ccggacatgg aggatctgtg tcgatttctc agggagagga
    18721 tagcatgaaa ggcagtccga cagccggcgg ggagggcctc ggtaataaat gagggtatcg
    18781 tggatcgtcc gtgggaccgc cggatgcgaa aaatatgctt ttaacgaata attattgata
    18841 gttacaaaga tatatatgat gtaattcgtc tttttttggg gttatcaaac atggacaaac
    18901 cgtccctaaa cgaggacaag ttcgtatgga aaaagaatga ccaggtggaa tacttcaagt
    18961 ctccggcctg ggaggcttat tcctttgtgg cccatgcctt ctgcacccgt cggggcggag
    19021 tcagctccgg gcgcttcgcc gcgctcaata tgagcggtcg ggaaggtgag gaggatgcca
    19081 atgtcagcgc caactgggat atcgccgctg cggccttcgg ccttacccgg cggcattttt
    19141 tccggattca tcaggtccac ggcgacaaga ttctcgttat cgacagcgcg gattttctca
    19201 ttcatacctg ccgccccctg gagtacgacg ccgtggttac aaatcggccg ggacttgccc
    19261 tgtgcatcct gaccgccgac tgtgtgcccg tattggtggc ggatccggcg cgacggattg
    19321 tcgccgcagt ccatgcgggt tggcggggta cggccctgaa tatttccggg caggtcgtga
    19381 cgctgctgcg ggaacgtttc gggagcagta ccagggatat ccgggcggct gtcggtcccg
    19441 ctatcggccc ctgctgctat gaggtggacg atgccgttcg ggcggccatg gaccggcatc
    19501 cgggccggga cgccattttt acgaaacaaa aggaaccagg taaatggcgc ctggatctgg
    19561 ctctcgccaa ccgcctccag atagaagccg caggggttac cgggggcaat atccaggtgg
    19621 cgaacctctg cacctcctgc cggcgggatc ttttctattc ccaccgcggc gaggggggga
    19681 agacgggccg actcctcaat ttcatcatga tttctccatg aagaaaattc cttgacatta
    19741 tggttgcgat cgggtaaaaa tacccacact ttcaaaaggc tcaggccagt acctaccgtc
    19801 cggagcatat ccggcgttca gatgagcaga ccttttgcaa tccactaaat ctttttatct
    19861 gttgttgcat ggtagattct cgttcataat attctgatga aaaaatataa tcagcaatta
    19921 tagctccagc ctcggtcccg gttgcctccg gtgccgtcta cataactaat ttagttagga
    19981 tattcacata tgaacatcga agatatcaaa aggcttccca ttagcgagct ggccaatctg
    20041 gccaaggatc tcaatgtggc cggtgccagc ggcatgcgtc gccaggactt gatctttgcc
    20101 atactgcagg cccagacgga aaaaaacggc ttaatctccg gtacgggggt gctggaaatc
    20161 ctccccgacg ggttcggctt cctccgggcg gtggattaca actatctgcc cagtcccgac
    20221 gacatctatg tatcgccttc acagatccgt cgcttcaatc tgcggaccgg cgataccatt
    20281 tccggcgagg tgcggccgcc caaggagggg gaaaaatatt tcgccctcct caaggtcgat
    20341 acggttaacc atgaattgcc cgacgcaacc cgggacaaga tcctcttcga caatctcacg
    20401 ccgctgtatc cggaggaaaa gttgaatctc gagctggatc ccgacaatct ttccacccgg
    20461 atcatggacc tctttacccc catcggcaag ggacagcggg gcctgatcgt ctcgccgccc
    20521 cgggcgggaa agacgatgct cctccaggat atcgcccgga gcatcgccgc caatcacaag
    20581 gaagtgacgc tcatggtcct cctcatcgac gaacgaccgg aggaagttac ggatatgcag
    20641 cggagcgtcc agggcgaggt cgtttcctcc accttcgacg aaccggcgac caggcatgtc
    20701 caggtagctg aaatggttat cgaaaaggcg aaacggctgg tggagcacaa aaaggacgta
    20761 gtgattctcc tcgacagcat tacccgcttg gcccgggcct acaacaccgt cgtgccaccc
    20821 agcggtaagg tcctctccgg cggcgtggat tccaatgccc tgcacaaacc caaacgcttt
    20881 ttcggcgccg cgcggaatat cgaaaacggc ggcagcctga ccatcatttc cacggcactt
    20941 atcgacaccg ggagccgtat ggacgaggtc atcttcgagg aattcaaggg gaccggcaac
    21001 atggaactgc atttggaccg ccgtatcgcc gaccggcgga tcttccccgc ctttgacctg
    21061 atccgttccg ggacgaggaa agaggaactc ctcatcccga aaaacaacct caaccggatc
    21121 tggatcctgc ggcggctcct tcaggagatg aacccggtgg acgccatgga tttcatcatc
    21181 gacaagatcc ggaaggcgga gaacaaccaa ctcttcctcg attccatgaa tcagtgacag
    21241 tcccgatgaa aaattttcct tgaatttatt tgtgccgtga ttataatgcg accccatttt
    21301 catcgggaga aaggttaaga caaaatgaaa gaaggcatcc atccccccta ttatgagacg
    21361 accatcactt gcgtctgcgg gaacgtgatc tctacccgct cgacgaaaaa ggacatcaag
    21421 gtggaaatct gttcccagtg tcatcccttc atgacgggga aacagaagat catcgatacg
    21481 gccggccggg ttgaacgttt ccacaagaaa tatgccggtg tcggcaaaaa ggtaggcaaa
    21541 tagagacggg ggtcatttcc gttttcccct gtcgagagga tttcccgtgc cggcgatgac
    21601 tgcaaaaaca gcgtggattc gggcgtttgt taggcatcga aggcattgga ttccagggcg
    21661 gcgcaagata ccctttgtgt cgttcgcgca ccatcccgtc atgcaaaggc aaacagactg
    21721 ccccagtttc cccgccgagg tcgacgccgc tgcaccattt ggtttccgcc caacgggcgg
    21781 gggtgtggtg gttccttcct aatgtttcct gtcttcaccg caccgaaagc gcggcttaat
    21841 gttcatccaa tcaggtttct attcgaccgg agcggggaag tattccgaaa acacggttta
    21901 atacttgtaa atcctcaaag gggtagtatc ggtgtcaatt tttgggaata tcattggaaa
    21961 tgtacagtag attaaaagaa atcgtcagga gatacgaaga gttacaggga cttttgagtg
    22021 acggcgagat tgtcgcaaag ccttcactct atcagaaata cgccaaagag catgccgatc
    22081 ttcatgatct cgtcgaggtt taccgggcct atgaagaggt gcgggggcga attgaagaag
    22141 gccaattcct cctgaaggaa aacgatgagg aactcaaaga gatcgttcgg gaggaaatgc
    22201 cgcttctcaa ggcgcaactg gaagaactgg acggccactt gaaggtcctt ctccttcccc
    22261 gggaccccaa cgacgacaag aacgtcctgc tcgaaatccg ggcggggacc ggcggcgatg
    22321 aagcgggact ttttgccgcc gacctctatc ggatgtacgg acgctacgcg gagcggagcg
    22381 gctggaagat ggaggtaatc agcaattccc cggcgggagg ggtcgggggg ttcaaggaaa
    22441 tcatcgccct catcgaaggc cgaggggcat acagccgcct gaagtacgaa agcggcgtcc
    22501 atcgagtcca gcgggtcccc gtaacggaaa cccagggtcg catccacacc tcggcggtga
    22561 cggtggccat ccttcccgag gcgga
//
LOCUS       DAOSMF010000023        21932 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_2977_length_21932_cov_20.754491, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000023 DAOSMF010000000
VERSION     DAOSMF010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 21932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21932
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2977_length_21932_cov_20.754491"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1784
                     /gene="recD"
                     /locus_tag="PLT63_04335"
     CDS             <1..1784
                     /gene="recD"
                     /locus_tag="PLT63_04335"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HOD97986.1"
                     /translation="CADFLTRLAVRLGAPPEAAATAGEAGRACLLALAAGHVCTDVEV
                     IAASLKTPAARIRELILNSRVAAPDDSGEILPLIIDGRGRIYLYRYFAYERRLAAAIT
                     DRLTAGQSLSPSGDDQQFLAARFAANAARLSGRPDRQRLAVAHALVNPLTIISGGPGT
                     GKTTIVTTLIAALAMREPPPRIALAAPTGKAAARMEAALGGMMEKIDPALASRLPKRA
                     STIHMLLGARPESGEFRHHRHNPLPCDVLIVDEASMLDLSLAARLFDALPADCRIVML
                     GDRDQLSAVEAGAVFAELAGEATGGDSPDAPPEREGKPGFSASSAPGARRPGLPDCVV
                     RLRENYRFDAASPIGKMAALVVGGDAGRLTAWLRELDDEALKWYPVGDELPARIIDTL
                     AAGFDAYAASVSRGDPGGALLAYENFCVLCVLRRGKRGVAGLNEAITRWLRPRLSGAP
                     GYDSPWYHGRPVMVTENDYALGVFNGDIGITLLGKDGRLEVWFSDREGKIRSVPPPSL
                     PAHQTAFAVTVHKAQGSEFENMALVLPETDLPVLTRELIYTAVTRVKRRLAVYGSLEI
                     LRAAVSRPTVRRSGLAERLAATTGRRS"
     gene            1809..2387
                     /locus_tag="PLT63_04340"
     CDS             1809..2387
                     /locus_tag="PLT63_04340"
                     /inference="COORDINATES: protein motif:HMM:NF024937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HOD97987.1"
                     /translation="MEPDPTNLLAFFIGIGISAACGFRLFLPFFGLSIAAINGWLNLS
                     PGFQWIGTWPALIAFATATVFEIGAYYIPWLDNLLDTVATPAAVIAGTIATVSVAGDL
                     PPFIKWSLALIAGGSVAGVVQTGTAVLRGASTMATGGTANFLLTTAEIFGSAFFTLLA
                     VAIPVLALIAALTLMIWLVWRRLHPRQSKNNI"
     gene            2514..3068
                     /locus_tag="PLT63_04345"
     CDS             2514..3068
                     /locus_tag="PLT63_04345"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOD97988.1"
                     /translation="MAVFYMWIFRWKKEGSVPYAPKFVMIAAPHTSNWDLPMTLILSL
                     AFRMKVFWLGKHTIFRPPFGGLFKWLGGIPVDRRQANNLVTQCIQTFRQSDRLIMIIP
                     PEGTRSKVRYWKTGFYYIAHGAGVPISLGYVDYKRKAGGMGPTVTTTGDIEADMMQIR
                     SFYAGVTGKNPHQWAEQAIGVTRK"
     gene            complement(3239..3910)
                     /locus_tag="PLT63_04350"
     CDS             complement(3239..3910)
                     /locus_tag="PLT63_04350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOD97989.1"
                     /translation="MDIHMRGNGGIQAVMFDFGGVIAEEGFKKGLSEIARKHGMAERE
                     FIQAAFDATYASGYVLGKATEADFWREVKRSTGLDGPDVSLWDYIYPHFIIRGWIQDL
                     VRSLKGKGLTVGILSDQTDMLDKLDERYDIFRQFDHVFNSFHLGKGKRDISLFDDVAA
                     LLRTEPGRLLFIDDHQGHVDRARQKGWQALLYVDRESLETDLERILTQDYSPKAEIYR
                     SSGRP"
     gene            4449..4694
                     /locus_tag="PLT63_04355"
     CDS             4449..4694
                     /locus_tag="PLT63_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97990.1"
                     /translation="MDYEEIQVQFYSGYKAIVRPMAFEYQGRRQEVEEILDRWYEGGV
                     DARRPEINYFKVRTSEGGIFLLRYHSVTDTWSACLRV"
     gene            5013..7931
                     /gene="hrpB"
                     /locus_tag="PLT63_04360"
     CDS             5013..7931
                     /gene="hrpB"
                     /locus_tag="PLT63_04360"
                     /inference="COORDINATES: protein motif:HMM:TIGR01970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HOD97991.1"
                     /translation="MPSYPIDHILPEVKAAVRANSAVIIQAPPGAGKTTRVPLALLEE
                     FSPRQGKIIMLEPRRIAAVAAARWMARQRGEPVGRTVGYAIRFDSRISADTRLEVVTE
                     GILTRRLLDDPTLDGVSLIVFDEFHERSLQADLALALCLDIQRGLRDDLKLLAMSATL
                     DIGPLAALLGDAPVISSPGRAFPVAERYLAAGDRGRERSREERVIGAVRTALAESPGD
                     ILVFLPGSGEIRRAATDLQGIPAVKGGEAAVHPLYGDLPFAEQERAIQPGRKRKIVLA
                     TNIAETSLTIEGIRVVIDSGLTRRLQYDPATGMNRLVTVKTSRAAAIQRQGRAGRIAP
                     GVCYRLYSPHDFQTMIPFAPPEILVADLSSLVLELASWGVTDPAALSWLDPPPEAAWT
                     AARELLSSLGALDRRGAPTELGREMARLPLTPRLGCLLLRAARRGFPGLGTDLAALLS
                     ERDIIRSGFRDPMFSGRNGDISERLFLLRRRRGEKMVREDAPDRLRRRQSRGDAPADG
                     CSVAGAPISDARRGVIGIAGVGSAPDGGVIAGADIGTSAGADKDVLMKYPAASFGKYN
                     PERLIDTDVGENGSAAAPVDPGALARVERAASQLRRLAAITVGGRAPDASGGRESGGG
                     KKRRASSKADHGGERYEGEMPAALLLHAYPDRIARRRDEGGDRYLLSQGRGVRLPRES
                     NMQGSVYLVVPRLDAGEKAEGIIHLAEAVTEETIRSELAGRLEVRRRVVWNRQEERIV
                     SLRQECLGALLLAETSFPAMDDEAVPLLCQAIVSGEARLDFRPEMRQFQGRVALLKRC
                     FPDEGWPDLSDAELLAAPEVWLAPRLAGVRTGAQLAALDLLPSLRALLPPAQQRLLEN
                     RAPTAVTVPSGRRVPLDYAAGPAPVLAVKLQEMFGLAATPTVADGRVAVRIHLLSPAG
                     RPVQVTEDLKSFWENGYPQVKKELKGRYPKHPWPDDPWHAAPTGRTQRRLSEKRKPGK
                     "
     gene            complement(7938..8435)
                     /locus_tag="PLT63_04365"
     CDS             complement(7938..8435)
                     /locus_tag="PLT63_04365"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HOD97992.1"
                     /translation="MEGKKVRESQVVLAQQMNPQDANPAGNVHGGVIMKMIDTAAGVA
                     AIRHSRSNAVTASVDRLDFYHPVFVGNLLSLRASVNFVGKTSMEVGVRVESENLLTGE
                     RLHTSTAYLTYVALDDQGRPLRLPPLILETETQKRRHQEAQTRRRMRLAEKTHEKSCQ
                     RDQEV"
     gene            complement(8532..9368)
                     /locus_tag="PLT63_04370"
     CDS             complement(8532..9368)
                     /locus_tag="PLT63_04370"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HOD97993.1"
                     /translation="MRKVFLPRNIAELWSVMADEPAARIYAGGTDLLVAMRSGAVVAP
                     ALICLERLPELQGVREEGEEMWLGAASTHADLLANPLVGRHLPLLAQAIRTLGSPPIR
                     NMGTIGGNVCTASPAGDTLPPLYALAAEVELQSAGSFRRLPVREFITGPGQTRLVTGE
                     ILTGIRVRKPVEANCHRFEKIGQRRALACCIASLAALLTVTPAGVVTSARLAWGSVGP
                     TVMEVPAAEAALTGAVMSRDTLLRAAAFARQAVTPIDDIRASAAYRREVAGNLLLRLL
                     PG"
     gene            complement(9365..9865)
                     /locus_tag="PLT63_04375"
     CDS             complement(9365..9865)
                     /locus_tag="PLT63_04375"
                     /inference="COORDINATES: protein motif:HMM:NF013923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOD97994.1"
                     /translation="MLAEKNTMTVEFNLNGRKIALDVDPDRRAVDVLREELGLTGTKE
                     GCGAGECGACTILVDGVNRLSCLMLAAQLAGREITTIEGLSREEGLHPIQEAFVKHGA
                     VQCGFCTPGMILGAAALLRANPVPTRDEIRRGISGNLCRCTGYQKIVDAVAEAASALK
                     EGEQSS"
     gene            complement(10164..12539)
                     /locus_tag="PLT63_04380"
     CDS             complement(10164..12539)
                     /locus_tag="PLT63_04380"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HOD97995.1"
                     /translation="MTIRVPEIGKDFIRPDAPAKAAGTEKYAVDHYGDNLLWAGVRRA
                     GVPHGLIRRVDVAAARSLPGVQAVLTGGDVPGTNRQGIVHKDQPVICDAKVRHAGDVV
                     ALVLAADRETLRRALALIEVDIEPLPGVFTIDDALNPAAPRLHEDHPGGNVLLQAAIE
                     VGDGAKALAECDVVVEETFYVPIQVHAFLETENGLARREEDGRIVIIVSTQAPFRDRW
                     EVGHALGLPMDKIRVIAPYLGGGFGGKDGATVQCLLALAAMHAGGRWVKMWWDREESF
                     QAGYKRHAARLHYILGAKADGTLHALHSRVYYDTGAYAHLGGEVMALGMEHAGGPYRI
                     PHACVEGWCLYTNNPVAGAMRGFGVAQVSFAFEGMMDILAARLGLDPLEMRRKNALRA
                     GDRNCAGVLMTTSTGIGECLDEIAGHPLWKDRAAWKAGAGPLKKRGVGLAAVFNAMGY
                     GRGLPDTAVAKLEITPAGRIVVYSGVADMGQGNATAFVQIAGAALNQEEGMIDLVQPD
                     TERTLPSGSAAASRTTYTYGNALIRACEDMRRRILGRASMFLLVDRLEDLELLPGRVR
                     HPATGREVPLAMLAATLTEPERFCVYQYLMPVVPDPPATGKAFKIGFPHLLFSYGCHL
                     AFIEADELTGELAVRDYLAVTEAGRIINPQTSVQQVQGAVAQGIGYALFEDLQVAGGI
                     IRTENLGEYIIPSALDLPDIRTAFVEVYEETGPFGMKGLGEVASNGPLPAIANALADA
                     GAARIFRSPLTPERVLTALRQGTPRLEIKLHDGQAVPGSDPGQMQRGRKQS"
     gene            complement(12745..13989)
                     /locus_tag="PLT63_04385"
     CDS             complement(12745..13989)
                     /locus_tag="PLT63_04385"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOD97996.1"
                     /translation="MPGILLLYIDGFSHLIAAQITVGITGLIGGLSYALVFGAMAKEI
                     PVRQTLSLSVAWALSLICGVSTTYLASGSSLKMLVVTFYSFAFWGMVGAFVTVRILRW
                     LVGEKNTVDMLPVLVILSLSPGIAATACDLIGEAMKMYLPGWLAWAISYEAMAVIIGC
                     GGAWAVIRTAKPSRTAEAAREPKMPAAAADNRLGITVLLLLCLPFYLNDFANIYIRDW
                     RLWLSMDYLGVKLFPLLVIGWMIRNRKITAAGLGLIWTPAVTFYSVFLVGTLIGIFLE
                     HSGYAIMSWLPGYRSLGKIPEIAIPLWNRIDLTAGLMLVAIIEELVFRGFLAAFLSRY
                     TESAAVIIGISALAFGMIHWSGGMHQVLITGIAGAVFMTLYLRTRSLPPLILSHFTIN
                     FIAFADIVPKSIFSFFRVTGNI"
     gene            14362..15177
                     /locus_tag="PLT63_04390"
     CDS             14362..15177
                     /locus_tag="PLT63_04390"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOD97997.1"
                     /translation="MDFKRNGNISEAQLVALKNWFSRYVRSFDAPDPVVRQALALKVD
                     HSLRVCDEILNIGGKLTLGDGELRLAKAMALVHDIGRFNQYFLYKTFVDKKSADHAQL
                     GVAALRDGNALAVIDNQASELILKAVSYHNRLHIPEDEGPACVFFTKLLRDADKLDIL
                     HLVSTYYYVPDSERNAAVELDLPDAPEVSDEILNTLLARKMVRMEQLRTLNDFKLLQM
                     AWVYDLNFPPTFQFVYRRKYLERIRDTLPASDGIDRIYAGLMSYLKEKSGSGS"
     gene            15334..16599
                     /locus_tag="PLT63_04395"
     CDS             15334..16599
                     /locus_tag="PLT63_04395"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HOD97998.1"
                     /translation="MARRLSPKKEPGDKRSVPEGMLTLPGAATGGPEPGGLLRLLVEK
                     RKRREPLALAVVMTCHGSTPRKPGTAMLVGADGAVTGTVGGGSLEAHIVAAAQQTIRE
                     GRSRCWTFSLTPRQAADDGMICGGVLEVLVDCLAAPDSLAGEILARALRAWEAGRDCW
                     LVYSLRTGEAFAAPAEKQAFPPAGEAARKDEAMGQGEAPDGQAVTIGLGLVSGDEFVI
                     GTLDTFGSDPKELEAAIQKDCRRRAVGLISMEPRGRYFLLPLAIPITVVIAGAGHVSR
                     ALVPLCRFAGFRTVVFDDRPEFAYRKFLPAADHIEITPSFDNCFQAVAAGPKDYIVIA
                     TRGHLSDAEVLAQALTTGAAYIGMLGSTKKREAIYRQLIREGCDPEALVRVRCPVGVT
                     IGAQTPEEIAVSIVAELIACRSAGRRTTV"
     gene            16831..17517
                     /locus_tag="PLT63_04400"
     CDS             16831..17517
                     /locus_tag="PLT63_04400"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOD97999.1"
                     /translation="MVSRRGATDDLTEAVTALIPAAGRSSRMGTCKPLLHLGEETVLE
                     RVIGIARQAGIGTVLAVLGHDADLLLPVLARHNVRGVINPDYEQGMFSSLRVGIRHLD
                     RNCPAFFLMPADMPLIRPDTLRRLLTAYRAGAPDILVCRPRYGNRRGHPPLISTALVS
                     DLLVHDGAGGLRTLLARFESATRNVDVEDPGILIDLDTPVDLTTCRRLTPAVTFDRPF
                     VAGSANRKGH"
     gene            17635..18951
                     /locus_tag="PLT63_04405"
     CDS             17635..18951
                     /locus_tag="PLT63_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98000.1"
                     /translation="MISTPSERTLTLQAPSLPSYIPVVVPFVENAAGAFGLGREDALK
                     LGLATEEIFAFLAGGICRGKPVEIECRSGIYYAGVEFRFSGTELNLGGLNITSGLNTA
                     ADGDLEDMGLLLASRTVDRLHISVGDDRRINLAVAKDKTYPPPGEGGLPAPDRGGEIT
                     VRTPNTEEIKEFSLRVAREPTELYRPPYFHYPGKVVDMIASGEYEATVAVTAGGNVAG
                     GLLHHFQTERIIQIIGPFSFAATDREGIERSVFDACLSRVARSRALGVVALHDLPASL
                     EGDFETLGRLCSYGEDGRASVQKTFGRLLNEDPGAVIWTAAELSSYLTGEYRRLALAR
                     EIKIVHDLGETRGGASIFSAEALRDRGNVTLRPLWPGADLTANVERHVHHLRGEGFRD
                     IFFLLDLGVPWHAALAPALLARGFRPETILPFGGRADVIIFQYHAT"
     gene            18941..20062
                     /gene="hisC"
                     /locus_tag="PLT63_04410"
     CDS             18941..20062
                     /gene="hisC"
                     /locus_tag="PLT63_04410"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOD98001.1"
                     /translation="MQPDLDFLTPAYVREFEPYVPSKPDAELKKLYGCTRLYRLNNNE
                     NALGPPPAAREAIRNFPSFRGALYPSGDSYYLRHEIAARHGLHPDQIIVGNGANEVIA
                     FVIKAFCAPGDNIITADRTFAVYEWVATFSGIMAKLIPLKKEGFDDEEMFRAVDSRTK
                     IIFLCNPNNPTGSYWGTERLTAFLTAAGGRRIVVVDEAYCEYVEAADFPDGMKLIADF
                     PNLVVFRTFSKMYGLGGLRIGYLAGAPEVVDMIRRTCVVYSVNGIAQEAALAALRDDA
                     DHIRRSRDLVRSSRAYLREELGKMDLPVITGEGNFLVVRLPGSDTLAYRRLMKEGVMI
                     RPMTSFRYPNHIRVTLAQPEAMEAFVAALKRLLSGKVAG"
     gene            20059..21249
                     /locus_tag="PLT63_04415"
     CDS             20059..21249
                     /locus_tag="PLT63_04415"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOD98002.1"
                     /translation="MNDANSPPRLLTYEFIVFCLITITTFCTVSIFYSFHHYLVEIGI
                     PPSWRGLIIGIEPMTAFILRLVILPWLQVRDTPAIIAASTVAMIFIGFGYLCATTVAS
                     LIVLRIFHGAAIVFLTVAVTALGVNFIPPERSGQAFGIFLVVTIAPYAFIPPMAEAIL
                     PYLRNEADLYAGVSIFVFFALLLLARGWKRIASYIAGMDGALLRRPTRVELYDNFSIA
                     TVRKMLWCFSLVYLIHATLFFFLKDLTLEITVGRVGTFFSIFMTMTIAVRLAGSRYFD
                     RVNKKKLLLAGFAAMATCFAVLPTAGSPGLYYLLAGLYGLGMGVIMPVMNALLFAASP
                     PALRGINVNMTMFTLDLGYFIMPYLGGLLIALGMGFSILFYAGAGFAVLAAAIVGTVK
                     TPGD"
     gene            21298..21759
                     /locus_tag="PLT63_04420"
     CDS             21298..21759
                     /locus_tag="PLT63_04420"
                     /inference="COORDINATES: protein motif:HMM:NF018437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HOD98003.1"
                     /translation="MMARKASLLCLAMLAGLLITQGCGKGSYKTKEKRIVRKMLVTGY
                     DAGQKSTGWKRKWGLFFMPPVYAYGPNEGKRKEVGVTADGTQAKKGVIAADINRYPYG
                     TKMYVPGYGWGEVHDIGTAIKGDHIDLFFPSEKEAKKWGRKYLKVIILLRE"
BASE COUNT         4242 a         6963 c         6483 g         4244 t
ORIGIN      
        1 gttgcgcgga tttcctcacc cggctcgccg tcaggctggg ggcgccaccg gaagctgcgg
       61 ccacggccgg ggaggcggga cgggcctgcc tactcgccct ggcggcaggc catgtctgca
      121 cggatgtgga ggttatcgcc gcaagcctga agacaccggc agcccgaatc agagagttga
      181 tactaaattc ccgggtcgca gcccctgacg attcgggaga gatactgcca ctcatcatcg
      241 acgggcgggg gcgcatctac ctttaccgct acttcgccta tgagcggcgt ttggccgccg
      301 ccatcactga ccgcctcacc gccggccagt cgctgtctcc ctccggagac gatcagcaat
      361 tcctcgcagc ccgtttcgcc gccaacgcgg cccggctctc cgggcgcccc gatcggcagc
      421 ggctggccgt agcccatgcc ctggtcaacc ccctgacgat catcagcggc ggccccggca
      481 ccggcaagac aaccatagtg accacgctga tcgcggccct ggccatgcgt gagccgccac
      541 cgcgcatcgc cctggcggcg cccaccggca aggcggcggc caggatggag gcggccctgg
      601 gcgggatgat ggaaaaaatt gatccggcct tggcatcccg tctgcccaaa cgggcgagca
      661 cgatccacat gctgctgggg gcccggccgg aaagcggtga attccgccat caccgtcata
      721 atccgctccc ctgcgacgtg ctgatcgtcg atgaagcctc catgctcgat ctgtctctcg
      781 ccgcccggct cttcgacgcc ctgccggcag attgccggat cgtgatgctc ggagacaggg
      841 atcaactctc ggcggtggag gccggtgcgg ttttcgccga attggcggga gaagctaccg
      901 gcggggattc cccggatgca ccgccggaac gggaaggcaa acccggattt tccgcgtcgt
      961 ccgctcccgg agcaagacgt ccgggattac cagactgcgt ggtcaggctc cgggaaaact
     1021 accggttcga tgccgcatcg ccgatcggca agatggccgc cctggtggtc ggcggcgatg
     1081 ccggccggct caccgcctgg ctccgggaac tcgatgatga agccctgaaa tggtacccgg
     1141 tgggagatga gctgccggca aggatcatcg acaccctggc ggcgggcttc gatgcctatg
     1201 cggcgtccgt gtcccggggg gaccccggcg gcgcgctgct ggcctatgaa aacttctgcg
     1261 tcctttgcgt cctccgccgg ggcaaacggg gggtggccgg gctcaacgaa gcgatcaccc
     1321 gatggctgcg cccacgactc tcaggcgccc ccggctacga ttccccctgg tatcatggac
     1381 gccccgtgat ggtcacggaa aacgactatg cgctgggggt gttcaacggc gatatcggaa
     1441 taactctcct tggcaaggac ggtcgtcttg aggtatggtt tagcgaccgg gaaggaaaaa
     1501 tccgttccgt accgccgcca tcgctgccgg cacatcagac ggcgttcgcc gtcaccgttc
     1561 acaaggcgca gggttcggaa ttcgaaaaca tggccctcgt cctgccggag acggatttgc
     1621 ctgtcctgac ccgggaactg atctataccg ccgtgacccg ggtaaaacga cgtctggccg
     1681 tatacggcag tttggagatc ctgcgggccg ccgtatcccg ccccaccgtc cggcgtagcg
     1741 gcctggcgga aaggctcgcc gcaaccaccg gccgacgctc gtgagccggg aaaagcgaag
     1801 ggagaaatat ggaacctgac ccaaccaatc tgctggcctt ctttatcggg atcggcatca
     1861 gtgccgcctg cggattccgg ctctttctcc cctttttcgg cttgagcatc gccgccataa
     1921 acggctggct caacctctcg ccgggtttcc agtggatcgg cacctggccg gccctgatcg
     1981 ccttcgccac ggcgacggtt tttgaaatcg gggcctacta catcccctgg ctggacaatc
     2041 tcctcgatac tgtcgccacc cctgccgccg tcatcgccgg caccattgcc accgtatccg
     2101 tcgccggcga tctgcctccc ttcatcaagt ggtccctggc cctaatcgcc ggcggcagcg
     2161 tcgccggggt cgtccagacc ggcaccgcgg tcctccgggg ggcctccacc atggccaccg
     2221 gcggcacggc caatttcctg cttaccacgg cggagatctt cggctccgcc ttcttcaccc
     2281 tgctggctgt cgccatcccg gtcctggcct tgatcgccgc tctgaccctg atgatctggc
     2341 tcgtctggcg gcgcctccac ccgcgccaaa gtaaaaataa tatttgattt ttcaactttt
     2401 acattttttt tacaatctta ccgattgaca ccggcgggcg tttacttgta gtcgcctttt
     2461 ccatgagacg aaccgttttc gatactccta tactgaagca cgtcctatac cttatggccg
     2521 tcttttacat gtggatcttt cgctggaaaa aggaaggcag cgttccctat gcgccgaagt
     2581 tcgtcatgat cgccgcaccc catacctcca actgggatct gcccatgacg ctcattctct
     2641 ccctggcctt ccggatgaag gtcttctggc tggggaaaca cacgatcttc cgccccccct
     2701 ttggcgggct gttcaagtgg ctgggaggca tccccgtgga tcggcggcag gccaacaacc
     2761 tggtaacgca atgcatccag accttccggc agagtgaccg cctcatcatg atcatccccc
     2821 cggaaggaac gcgttccaag gtgcgttact ggaagacagg attctactac atagcccacg
     2881 gcgccggcgt ccccatctcc ctggggtatg tggactacaa gcgcaaggcc ggcggcatgg
     2941 gacccaccgt gaccacaacg ggagacatcg aagcggacat gatgcagatc cgctccttct
     3001 acgccggggt gacgggcaaa aatccccacc agtgggcgga acaggccatc ggtgtcaccc
     3061 gaaaatgaga tgcggcagcg gaaaaataag gcccttaacc tttagctctt gcggctggtt
     3121 ttggttctga aagagtatgt ggaccggggg ggggtaattg gggcagaaat cggggaaggg
     3181 cttccatgaa tatccccatc cgtcatttca ggcccccggt tttttgtagg ggaaatgatc
     3241 atggccggcc ggatgatcta tatatttctg ctttcgggga gtaatcttgc gtcagtatcc
     3301 tttccagatc cgtttccagg gattcccgat caacatatag cagcgcctgc caaccctttt
     3361 gccttgcccg gtcgacgtgc ccctgatgat cgtcgatgaa gagcagtctc cccggttcgg
     3421 tccgcaatag ggcggcgacg tcgtcaaaaa gcgaaatatc ccgcttgcct ttaccgagat
     3481 ggaaactgtt gaagacatga tcgaactgcc tgaaaatgtc atagcgttca tcgagtttat
     3541 cgagcatgtc cgtctgatcg ctgagaatgc ctaccgtcaa gccttttccc ttcaggctcc
     3601 gaactaggtc ctggatccag ccccggatga tgaaatgggg gtagatataa tcccacagcg
     3661 aaacatccgg accatcgagg ccggtgctcc ttttcacttc cctccagaaa tcggcttccg
     3721 tggccttccc gaggacatag ccgctggcat aggtggcgtc gaatgccgcc tggatgaatt
     3781 cccgctccgc catgccatgc ttcctggcga tttcggagag accttttttg aatccttcct
     3841 cggcgattac cccgccgaaa tcgaacatga cggcctgaat cccaccgttg ccccgcatat
     3901 gtatgtccat ggagaatcct cgctgaattt cttgccggcg ccgatagccc cgttccagcg
     3961 cgccaccaga tcggtcatcg caacggtaac ataaccgatt tcattacgat tgcaagtaaa
     4021 aattcgcgga aaagagggaa agaatttgcc aagatcatct ttacttgggc ttgtcaatgg
     4081 gagcgatgta aaaagaaagc ccccgtccaa ctcaatggag ggaggctttc ggaaatctcg
     4141 ccgatgcaga gcggttactt cacatggatc ggcgtgcagg tgggatcaat ccaacccttc
     4201 cacaattttt tagtctcttt tgcctttttc atgatcgttt tctcctctct cgataaattt
     4261 tttgttccgg cggttgcctt tcttttcttc tctcgcctgt agaatagttc tatccgtaat
     4321 ttttaacaat cagcggcgag ctgattatca aatgggaatt agaatggata tggtgtaaaa
     4381 taacgcatct tcattctatc ggcatttgtc ttatggaagg cgacaagata aagtcaggag
     4441 aggcaatcat ggattacgaa gagattcagg ttcaatttta cagcggatat aaggccattg
     4501 tccgcccgat ggcttttgaa tatcagggac ggcgccagga ggtggaggag atattggatc
     4561 gctggtatga aggcggcgtc gatgcccgcc gcccggaaat caactatttc aaggtcagga
     4621 cttcagaggg ggggattttt ctgctgcgat accattcggt caccgacacc tggtccgcct
     4681 gccttcgagt ttaaagaaaa ctagaaaaac gaggcgaaat cacttgatcc atatatttat
     4741 ctggaggtat ttgattttta tttgcgaaaa ataagaactt gctgacccat aaagcgtgaa
     4801 agaagacatg attttccggc gccaatttct tatccaaaag aaagtcttgg aactccgtaa
     4861 gaacgtcggt attttttgca ttggacaaat acaccataaa ttacccgccg gccttttagt
     4921 ctttattacc acaaaatact ctacgctaag attttataaa cggataattt ccgccagggg
     4981 atgatgcaat cccttaaaaa aaccatttta ctatgccatc ctatcccatc gaccatatcc
     5041 tgccggaggt gaaagcagcc gtccgggcca attcggcggt gatcatccag gccccgcccg
     5101 gggcggggaa gacgacccgg gtgccactgg cgcttctgga agaattttct ccccgccagg
     5161 gaaagatcat catgctggag ccccggcgga tcgccgcagt ggcggcggcc cggtggatgg
     5221 ccaggcagcg cggcgaaccc gttggccgga ccgtgggcta cgccatccgg ttcgacagca
     5281 ggatctccgc ggacacccgc ctggaggtgg tcacggaagg catcctcacc cggcgcctcc
     5341 tcgacgatcc cacccttgac ggcgtctccc tgatcgtctt cgacgagttt cacgagcgga
     5401 gtctgcaggc cgacctggcc ctggcccttt gcctcgacat ccagcgggga ctccgggatg
     5461 acctcaagct actcgccatg tcggcgaccc tcgacatcgg accgctggcc gccctgctgg
     5521 gcgacgcccc ggtgatttcc tcccccgggc gggcctttcc agtggcggag cgctaccttg
     5581 ccgccggaga ccggggccgg gagagatccc gggaagaacg ggtgatcggc gccgtccgca
     5641 cggccttggc ggagagcccc ggagacatcc tcgtcttcct tcccggcagc ggagagatcc
     5701 gccgggccgc cacggacctg cagggcatac cggcggtaaa gggtggagag gcggccgttc
     5761 atcctctcta cggagatctc cccttcgccg agcaggagcg ggccattcaa cccggtcgga
     5821 agcgaaagat cgtcctggcc accaacatcg ctgaaacgag cctcaccatt gaggggatcc
     5881 gggtggttat cgatagcggc ctgacccgcc gcctccagta cgatccggcc acggggatga
     5941 accgcctggt caccgtcaag acctcccggg cggcggcgat ccagcgccag ggtcgggccg
     6001 gtcgcatcgc ccccggcgtc tgttaccgcc tctacagtcc ccatgatttt cagaccatga
     6061 tcccctttgc cccgccggag atcctggttg cggacctttc ctccctggtg ctggagttgg
     6121 cgtcctgggg cgtcaccgat cccgccgccc tctcctggct cgatccgccc ccggaggcgg
     6181 cctggaccgc ggccagggag ctgctgtcaa gcctgggcgc cctggaccgc cggggcgcgc
     6241 ccacggagct cggaagggaa atggcgcgcc tccctcttac tccccgcctg ggatgcctgc
     6301 ttctgcgggc cgcccgccgc ggctttcccg gcctcggcac ggacctcgcc gccctgcttt
     6361 ccgagcggga catcatccgt tcgggtttcc gggatcccat gttttcggga aggaacggcg
     6421 acatcagcga acgcctcttt ctcctccggc gtcgccgcgg ggaaaaaatg gtccgggaag
     6481 atgcccccga ccgcctccgg cggcggcaat cccgcggcga tgccccggcg gatggttgtt
     6541 cagtcgccgg cgctcccatt tcggacgcac gaaggggtgt aatcggcata gccggcgtgg
     6601 gcagcgcccc ggacggggga gtgatcgccg gcgccgatat cggcacaagc gcgggcgccg
     6661 acaaagacgt cttaatgaaa taccccgcgg caagcttcgg gaaatataac cctgagagac
     6721 ttatcgatac cgacgttggg gaaaacggct cggccgccgc accagtggac ccgggggccc
     6781 tggcaagagt ggaacgggcg gcatcgcagc tccggcggct ggcagcgatt acggtcggcg
     6841 gtcgtgcccc ggacgcttcg gggggacggg aaagcggcgg tggtaagaag cggcgcgcct
     6901 catccaaagc ggatcacggc ggggaacgat acgaagggga aatgccggcg gcgctgctcc
     6961 tccacgccta tcccgatcgg atcgcccgtc gtcgcgacga aggcggggac cggtatctcc
     7021 tcagccaggg acgaggcgtc cgcctccccc gggagagcaa tatgcaagga agcgtctatc
     7081 tcgtcgtccc ccggctcgac gccggggaaa aggcggaggg gataatccac ctggccgaag
     7141 cggttaccga ggaaaccatc cggagcgagc tggccggccg cctggaagta cgtcggcggg
     7201 tggtctggaa ccggcaggaa gagaggatcg tctcattacg gcaggagtgt ctgggcgccc
     7261 tgctcctcgc ggaaacgtcc tttccggcca tggatgacga ggccgtgccg ctcctctgcc
     7321 aggcgattgt atcgggcgag gcccggcttg atttccgccc ggaaatgcgg cagtttcagg
     7381 gtcgggtggc cctgctgaag agatgtttcc ccgacgaagg ctggccggat ctatcggatg
     7441 cggaactcct cgccgccccg gaagtctggc tggctccgcg gctggcgggc gtccgcaccg
     7501 gcgcccagct tgccgctctc gatctgctcc cctccctgcg ggcgctcctg cccccggcgc
     7561 aacagcggct cctggagaac cgggcaccca ccgccgttac cgtacccagc ggccgccgcg
     7621 tccccctgga ttacgccgcc ggcccggcgc cggttctggc cgtaaagctc caggagatgt
     7681 tcggccttgc cgccaccccc accgtggcgg atgggcgggt ggcggttcgc atccacctcc
     7741 tctcccccgc cgggcggccg gtacaggtca ccgaagacct gaaaagcttc tgggaaaacg
     7801 gctatcccca agtgaagaag gaattgaagg gacgctaccc caaacatccg tggcccgacg
     7861 atccctggca cgccgcgccc accggccgca cccagcgccg cctttccgaa aaacgcaagc
     7921 ccgggaagtg aagcgcttca gacctcctga tcccgctggc aggacttctc gtgggtcttt
     7981 tccgccagcc gcatccgccg ccgtgtctgc gcctcctggt ggcgccgctt ctgggtttcc
     8041 gtttccagga tcagcggcgg cagccgcagc ggtctcccct ggtcatccag ggccacatag
     8101 gtcagatacg ccgtcgaggt atggaggcgt tccccggtga ggagattctc cgactctacc
     8161 cggaccccca cctccatgga ggtcttcccg acaaagttca cactggcccg tagggagagc
     8221 agattgccga caaaaaccgg gtgatagaaa tccaggcggt ccaccgaggc ggtaacggca
     8281 ttggaccggg agtgccggat ggccgccacc cccgccgccg tgtcgatcat cttcatgatg
     8341 acaccgccgt ggacgtttcc cgccggattg gcatcctggg ggttcatctg ttgcgccaaa
     8401 accacctgcg attccctcac cttctttcct tccatccttg acctcccgga aaaataacaa
     8461 ccgccgcgcg gccgttatta agtttgtgtg gttgtgtgtg agcgccgtga aaacaaacgg
     8521 ccccggccgc tttatcccgg cagcagtctc aacaggagat ttccggccac ttccctgcgg
     8581 taggcggcgg aagcccggat atcgtcgata ggcgtcacgg cttggcgggc aaaagcggcg
     8641 gcccggagca gggtatcccg gctcatgacg gccccggtca gggccgcttc cgccgccggc
     8701 acttccatta ccgtcggccc cacgctgccc caggcgagcc gggcggaggt cacgacaccc
     8761 gccggcgtca ccgtgagcag ggccgccagg ctggctatgc agcaggcgag ggcccggcgc
     8821 tgcccgatct tctcgaaccg gtggcaattg gcttccaccg gtttccggac gcggatgccc
     8881 gtgaggatct cccccgttac caggcgggtt tgtcccggtc ccgtgatgaa ctcccgcacc
     8941 ggcagccggc ggaagctccc cgcgctttgg agttccacct ccgcggcgag ggcgtagagg
     9001 ggaggcaagg tgtctccggc gggggaggcg gtgcagacat tgccgccgat ggtgcccatg
     9061 ttgcgaattg gcggcgatcc cagggtccgg atggcctggg ccaaaagggg cagatgccgc
     9121 ccgaccaggg gatttgccag caggtcggca tgggtggagg cggcacccag ccacatttcc
     9181 tccccctctt cccggacccc ctggagttcc ggcagacgtt ccaggcagat cagggcgggg
     9241 gcgacgacgg ccccggaccg catcgccacc aacagatccg tcccgccggc atagatccgg
     9301 gccgccggct cgtccgccat gaccgaccac agttcagcaa tgttccgggg gagaaaaacc
     9361 ttcctcatga ggattgttct ccctctttca acgcgcttgc cgcctcagcc acggcatcga
     9421 cgatcttctg gtagcccgtg cagcggcaga ggttgccgct gatcccccgg cgaatctcgt
     9481 cgcgggtcgg gacggggttc gcccggagga gcgccgcggc gcccaggatc atccccgggg
     9541 tgcagaaccc gcactgtacg gcgccgtgct tcacgaaggc ctcctggatg ggatggagtc
     9601 cttcctcccg ggacagtccc tcgatggtgg tgatttcccg gcccgccaac tgagcggcca
     9661 gcatgagaca ggacaggcga ttgacaccgt ccacgaggat cgtgcaggcc ccgcattccc
     9721 cggcgccgca gccctccttc gtgccggtga gacccaactc ctcccggagg acatcaacgg
     9781 cccgccggtc cgggtccacg tcgagagcga tctttctgcc gttgaggtta aattccaccg
     9841 tcatcgtgtt tttctccgcc aacatcaaaa gtgataacca tcctcatttc atctaaaaat
     9901 gcgcttgtat atgcccgttt acatttacta ttatcatttc atccccttcg aggggaggat
     9961 tgtgatgggg atgcgtcttt cattatcgcg cccaactcta tacatagcac catactgaac
    10021 aggttgtatg ttatcgtccg acttgaccgg acgatccagt gttttatgga ttccccgatc
    10081 aagtcgggga atgactgcgc gtatccgcgc tacattttat gaacatcaag cttcgctttc
    10141 atcccccttt gtgacggatg ccgtcatgat tgtttccgcc ccctttgcat ctgtcccgga
    10201 tctgatccgg gaacggcctg gccgtcatgg agttttatct ccaagcgcgg cgtcccctgc
    10261 cggagggccg tcaggacccg ctccggcgtc aggggagagc ggaaaatccg ggcggcgccg
    10321 gcgtcggcca gggcgttggc gatcgccggg agcggcccgt tggacgccac ttcaccgagg
    10381 cccttcatcc cgaaaggtcc cgtttcttca tatacttcta cgaaagcggt ccggatatcc
    10441 ggcagatcga gggcggaggg gattatgtac tccccgagat tctccgtgcg gattatcccc
    10501 cccgccacct ggaggtcctc gaaaagggca tagccgatcc cctgggcgac ggcgccctgg
    10561 acctgctgca cgctggtctg gggattgata atccgccccg cctccgttac ggccaagtaa
    10621 tcccggaccg ccagctctcc cgtgagctcg tccgcctcta taaaggccag gtggcagcca
    10681 taggagaaca gcagatgggg gaacccgatc ttgaaggcct ttcccgtcgc cggcgggtcc
    10741 ggaaccaccg gcatgaggta ctggtacacg cagaaccgct ccggctccgt cagggttgcg
    10801 gccagcatgg ccagggggac ctcccgcccc gtcgccggat gacgaacccg gccgggaagc
    10861 agttccaaat cctccagccg gtccacgagg aggaacatgg aggcccggcc caaaatccgt
    10921 cggcgcatat cctcgcaggc acgtatgagg gcgttgccgt aagtgtaggt ggtccggctc
    10981 gcggcggcgg aacccgaggg gagggtccgt tccgtatcgg gctggaccag atcgatcatg
    11041 ccctcctcct ggttgagcgc cgccccggcg atctggacga aggccgtggc gttcccctgc
    11101 cccatgtcgg cgacgccgct gtaaacgacg atccgccccg ccggggtgat ctcgagcttg
    11161 gccacggcgg tatcgggaag accgcggcca tagcccatgg cattgaaaac cgccgcaagc
    11221 cccacgcccc gcttcttgag cggcccggcg ccagccttcc aggcggcgcg atccttccag
    11281 aggggatgac cggcaatctc gtccaggcac tccccgatgc cggtggaggt ggtcatgagg
    11341 acgccggcgc agttccggtc gccggcccgg agggcgtttt tacggcgcat ttccaggggg
    11401 tccagaccca gccgcgccgc caggatgtcc atcatgccct cgaaggcaaa gctcacctgg
    11461 gcaaccccga agccccgcat ggccccggcg acgggattgt tggtgtagag acaccaacct
    11521 tcgacgcagg catggggaat ccggtagggc ccgccggcgt gctccatccc cagggccatg
    11581 acctcgccgc ccagatgggc gtatgccccg gtgtcgtaat agacgcggct gtggagggcg
    11641 tggagggttc cgtcggcctt cgcgccgaga atgtagtgaa ggcgggcggc atggcgtttg
    11701 tatcccgcct ggaagctttc ctcccggtcc caccacatct tgacccaccg gccgccggcg
    11761 tgcatcgccg ccagggccag gaggcactgg accgtcgccc cgtccttgcc gccgaagccg
    11821 ccccccagat agggagcgat gacgcggatt ttgtccatgg gcaggcccag ggcatggccg
    11881 acctcccacc ggtcccggaa cggggcctgg gtggagacga tgatgacgat ccggccgtcc
    11941 tcctcccgcc gggcgaggcc gttttccgtt tccagaaagg catggacctg gatcggcaca
    12001 tagaatgtct cctcgacgac aacatcgcat tccgccaggg ccttcgcccc atcccccacc
    12061 tcgatcgccg cctggaggag gacgttgcca ccgggatggt cttcgtgaag gcgcggcgcg
    12121 gcggggttca gggcgtcgtc gatggtgaaa acgccgggga gaggttcgat atccacctcg
    12181 atgagagcca gtgcccggcg caacgtctcc cggtccgccg ccaggaccag ggcgacgacg
    12241 tcgccggcat ggcggacctt ggcgtcgcag atcaccggct gatccttgtg gacgatcccc
    12301 tggcggttgg tcccgggcac gtccccgccg gttaggacgg cctggacccc ggggagcgac
    12361 cgggccgcag cgacatccac ccggcggatc aggccgtggg gaacccctgc ccggcggacc
    12421 cccgcccaga gcaggttgtc gccatagtga tccacggcgt acttctccgt tccagcggcc
    12481 ttggccgggg cgtcgggacg gataaagtcc ttgcctattt caggtacacg gatcgtcatg
    12541 ataagaacgt ggcgaatgta gcgcaattgc cggccatttc ccacccctga ccgtaattat
    12601 agtcgaaacg cttgaaattg tccaaatcaa ggctgtcagc tcatagatca gttcccacca
    12661 ggtccttgtc cgctcgctca tattggaact ccgttaagaa tagcctcttc agctttcatt
    12721 tcccgaaaat aagcacgttg tcaatcagat attgcccgtg accctaaaaa aagaaaagat
    12781 ggatttcgga acgatatctg caaaagcaat aaaattgatg gtgaaatggg aaagaatgag
    12841 cggggggagg gaccgcgtcc tcaggtacag agtcatgaag accgcgccgg caatacccgt
    12901 tatcagcacc tggtgcatac cgccgctcca gtgaatcatg ccaaaggcca acgccgatat
    12961 gccaatgatg acggcggcac tttcagtgta gcgggaaaga aacgccgcca gaaagccacg
    13021 aaaaaccagt tcctcgatta tcgccacaag catgagaccg gcagtaagat cgatccggtt
    13081 ccagagcgga atcgcaatct ccggtatctt tcccagagat cgatagcccg gcaaccagct
    13141 catgattgca tagccactat gctccaggaa aatgccgatc agtgtcccga caagaaacac
    13201 gctgtagaag gtaacggccg gcgtccatat caaccctaat cccgcagcgg taatcttccg
    13261 gttccgaatc atccatccga tgaccaacaa cggaaaaagc ttcaccccga gatagtccat
    13321 ggagagccaa agacgccaat ccctgatgta gatattggca aaatcattca gataaaaagg
    13381 gaggcaaagc aagagcagga ccgtgatgcc aagtctgtta tccgccgctg ccgcgggcat
    13441 cttcggttcc cgcgccgctt ccgcagtccg actgggtttc gctgtccgga taacggccca
    13501 agcgccacca caaccgatga tcacggccat tgcctcatac gaaatcgccc atgccagcca
    13561 tccgggtaaa tacattttca tggcttcgcc gatcaagtcg caggcagtcg cggcaattcc
    13621 cgggctcaaa gacaagatga cgagcaccgg cagcatatct accgtgtttt tttccccaac
    13681 cagccaccgc aaaatgcgca ccgtgacgaa agctcccacc atcccccaga aggcaaaact
    13741 gtaaaacgtg acgaccaaca tcttcagcga tgatcccgag gcaagatagg tcgtagaaac
    13801 tccgcagatt agactcaacg cccatgccac cgacagagat agagtttgcc ttacggggat
    13861 ctccttcgcc atggcaccga aaaccaaggc gtacgacaag cccccgataa gtccggtgat
    13921 tcccaccgtg atctgggcgg cgatcagatg ggagaatccg tcaatataca acaacagaat
    13981 gcccggcata cccacgatcc cggcgaatga ccatcccaag atcatgagca acagcgggga
    14041 tctttttatg ctcgcacttt tcatatccag gctgaaacgt tttcaatttt atgggcagcg
    14101 atttatcaac tttttatcgt ccctgtattt gccatcaacg gggaagtcaa gcagaacaca
    14161 tcgtaaacgg cttgtttacc gcctcgtcag atgcaaaaaa gaagttgaca cggtggctca
    14221 acctctatag aaacccaagc ttgtcaccgc cgacacgaat tttgtggcga ttatcccgcc
    14281 cgcgagcttt atccgtaaac ataaatcatc tcccagcggg ccagccgcca tgtccggcta
    14341 ctgtcccgtt agaggcgtcg catggatttc aagcgcaacg gaaacatctc ggaagcacaa
    14401 ctcgtcgctc tgaagaactg gttttccagg tatgtccggt cctttgacgc cccggacccg
    14461 gtggtacggc aggcgctggc gctgaaagtg gatcattccc tccgggtttg cgacgagatc
    14521 ctgaacattg gcggcaagct cactcttggc gacggcgagt tgcgccttgc caaagccatg
    14581 gccctggttc acgatatcgg caggttcaac caatacttcc tttacaaaac cttcgtggac
    14641 aagaaatcgg cggatcacgc ccaactgggc gtcgcggcgc tccgggacgg aaacgccctg
    14701 gccgtaatcg acaaccaggc cagcgagctc atcctgaaag ccgtatccta tcacaaccgc
    14761 ctgcatatcc cggaagacga aggccccgcc tgcgtcttct tcaccaaatt gctccgggac
    14821 gccgacaagc tggacatctt acatctggta tcgacctact attacgtgcc ggactcagaa
    14881 agaaacgccg ccgttgaact cgatctgccc gatgctcccg aggtgtccga tgaaatcctc
    14941 aataccctcc tggcgcggaa gatggttcgc atggagcaac tgcggaccct gaacgacttc
    15001 aagctcctgc agatggcatg ggtttacgac ctcaacttcc cacccacctt ccagtttgtt
    15061 taccggcgca aatatctcga aaggatacgg gacaccctgc cggcgtctga cgggatagat
    15121 cgaatttatg ccggactgat gtcctacctc aaggaaaaat cgggttccgg aagttaagta
    15181 cgcctccatc ttcaagaccc tgcgcggtca ccaacatcgg cggtccggca aggcagcgga
    15241 tcttccgctc agcatgaagt cagatccgcc tcgtcggccg cggaccggcg gaattgaaaa
    15301 aaggcagcga aaatcgggaa aggtcagcaa ataatggcaa ggcgcctcag tccgaaaaaa
    15361 gagcccggtg acaaacgatc cgtccccgaa ggaatgttga cgctacctgg agcggcaacc
    15421 ggcggtccgg aacccggcgg tctgttgcgt ctcctcgtgg aaaaaaggaa acgccgggaa
    15481 ccgctggcgc tggccgtcgt catgacctgc cacggctcca cgccccgcaa accggggacg
    15541 gccatgctcg tcggcgccga cggcgcggtg acggggacgg tgggtggtgg ttcgctggag
    15601 gcgcatatcg tggccgcggc ccagcagacc atccgggagg gacgatcccg ctgttggacc
    15661 ttctccctga caccccggca ggcggcggac gatggaatga tctgcggtgg cgtcctggag
    15721 gtcctggtgg attgcctggc cgcccccgat tcccttgcgg gggaaatcct ggcccgggcc
    15781 ttgcgggcat gggaagcggg ccgggactgt tggctggtgt attcactgcg gaccggcgaa
    15841 gcctttgcgg cgccggcgga aaagcaggcc ttcccgccgg cgggggaggc ggcccggaaa
    15901 gacgaagcaa tgggacaggg ggaagcgccg gatggccaag cggtaacgat cggcctcgga
    15961 ctcgtgagcg gcgacgagtt cgtgatcggc accctcgata ccttcggatc ggacccgaag
    16021 gagcttgaag ccgcaatcca aaaggattgc cgccgccgtg ccgtgggcct gataagcatg
    16081 gaaccccggg ggcgctattt cctcctgccc ctggccatcc cgatcacggt cgttatcgcc
    16141 ggcgccggcc acgtctcccg ggcgctggtc cccctctgcc ggtttgccgg ttttcggacg
    16201 gtggtcttcg acgaccgccc ggagttcgcc taccggaaat ttctcccggc ggcggaccat
    16261 atcgagataa cgccttcttt cgacaactgc ttccaggcgg tggcggcggg gcccaaggat
    16321 tatatcgtca tcgccacccg gggccacctc agtgacgccg aggtcctcgc ccaggccctc
    16381 accaccggcg cggcctacat cggcatgctc ggcagcacaa agaagcggga ggccatctac
    16441 cggcaactca tccgggaggg atgcgacccg gaagccctgg tccgggtccg ctgtcccgtc
    16501 ggcgtgacca tcggcgccca gaccccggag gagatcgccg tgagcatcgt cgccgagctt
    16561 atcgcctgcc gctccgccgg gcggcggaca acagtttgac ggtccgaccc ggacaaaccg
    16621 gaaccggaaa agatgaacgc ttagaaagcc attttccttt ctttccaggg gggaggagac
    16681 tagaaggaaa aacccctttt tattcccctt ccagggagga gattaggcgg tagaacccat
    16741 ttttatcctc cccttccaag ggggagatag agaggttgat agtctttgta ttcctcccct
    16801 tcaaagggga ggttaggagg ggggtgagac atggtttccc gcagaggtgc tacagatgat
    16861 cttaccgaag cagttacggc gctgattccc gccgccggac gatcgtcccg gatggggacc
    16921 tgtaaacccc tgctgcatct cggtgaggag acggtgctgg aaagggtgat cggcatcgcc
    16981 aggcaggccg ggataggaac cgtcctggcg gtcctgggcc atgacgcaga cctcctgtta
    17041 ccggtcctgg cgaggcacaa tgtccgtggg gtgatcaatc ccgattatga acagggcatg
    17101 ttttcctcgc tccgcgtcgg catcaggcat ctggacagaa attgccctgc ttttttcctc
    17161 atgccggcgg acatgcccct gatccggccg gacaccctcc gccggctcct gaccgcttac
    17221 cgggcggggg cgccggatat tctcgtctgc cgtccccgtt acggcaaccg ccggggccac
    17281 ccgccgctca tctcgactgc cctggtttcc gacctgcttg tccatgacgg cgccggcggc
    17341 ctccggacct tgctggcccg ctttgaaagc gccacccgga acgtggatgt ggaagatccg
    17401 gggatcctga tcgacctgga tacgccggtg gatctgacga catgccgccg gttgaccccg
    17461 gcggtcactt ttgaccggcc gttcgtcgcc gggtcggcca acaggaaagg acattgacag
    17521 cgccggatat ttctcatata tccgcatcgt aagctgcagg tccggcaaga ccggaaaaga
    17581 tattgattca ccaggccgct tttcacaagt cccggaccgg aaaggatcga cgccatgatc
    17641 agcaccccgt cggaacgtac cctgaccttg caggctccct ccctgccctc ctacatcccc
    17701 gtggtcgtcc ccttcgtgga aaatgccgcg ggggcgtttg gcctgggtcg ggaggatgcc
    17761 ttgaagctgg gactggccac cgaagagatc ttcgccttcc tcgccggcgg gatctgccgg
    17821 ggaaaaccgg tggagatcga atgtcggagc gggatttact acgccggcgt agagttccgg
    17881 ttttccggga cggaattgaa cctcgggggg ctcaacatca cctccggctt gaatacggcc
    17941 gccgacgggg atctggaaga catggggctc ctactggcgt cccgcaccgt ggaccgcctc
    18001 catatcagcg tgggcgacga ccgccggatc aacctcgcgg tcgccaagga caagacctat
    18061 ccaccgccgg gggaaggcgg cctgcctgct cccgaccgcg ggggggaaat tacggtaaga
    18121 acccctaata cagaggaaat aaaagagttt tccctccggg tggcccggga gccgaccgaa
    18181 ctttaccgac cgccctattt ccactatccc ggcaaggtgg tggatatgat cgccagcggc
    18241 gaatacgagg cgaccgtcgc cgtcaccgcc gggggaaatg tagccggggg cctcctccat
    18301 cacttccaga cggagcggat catccagata atcggtccgt tctcgtttgc cgccacggat
    18361 cgggaaggca tcgagcggtc ggtgttcgac gcctgtctat cccgggtggc gcggagccgt
    18421 gccttgggcg tcgtcgccct ccacgacctc ccggcgtccc tggaggggga ttttgaaacc
    18481 ctgggccgcc tgtgttccta cggcgaggac ggccgggcaa gcgtacagaa gaccttcggg
    18541 cgcctgttga acgaagaccc gggcgccgtg atctggaccg cggcggaact ctcctcttat
    18601 ctcaccggcg aataccgccg gctggccctg gcccgggaga tcaaaatcgt ccatgatctg
    18661 ggagaaacac ggggcggcgc ctcgatattc tccgccgagg cgttgcggga ccgcggcaat
    18721 gtcaccctcc gtcccctctg gcccggcgcc gacttgacgg cgaacgtgga aagacatgtc
    18781 caccacctcc ggggagaagg tttccgggat atcttcttcc tgctggacct gggcgtccct
    18841 tggcacgccg ccctcgcccc ggcactgctg gcgcggggat tccgtccgga gacgatcctc
    18901 cccttcggcg gccgcgccga cgtgataatc tttcaatatc atgcaacctg atctggattt
    18961 cctgacgccc gcctatgtgc gggaattcga accctatgtc cccagcaagc cggacgcgga
    19021 gctgaaaaag ctctacgggt gcacccggct ctaccgtttg aacaacaacg aaaacgccct
    19081 ggggccgccg ccggcggccc gggaggcgat ccggaatttt ccgtcgttcc gaggcgccct
    19141 ctaccccagc ggcgattctt attatctccg ccacgaaatc gccgcccgtc atggtctcca
    19201 cccggatcag atcatcgtgg gcaacggcgc caacgaggtg atcgcctttg taatcaaggc
    19261 cttctgcgcg ccgggcgaca acatcatcac cgccgaccgg acctttgccg tctatgaatg
    19321 ggtggcgacc ttttccggta tcatggcaaa attgatcccc ttgaagaagg aaggattcga
    19381 cgacgaggag atgttccggg ccgttgacag ccgcaccaag atcatcttcc tgtgcaaccc
    19441 caacaacccc accggcagct actggggaac ggaacggctc acggcctttc ttacggccgc
    19501 cggggggcgg cggatcgtag tcgtggacga ggcgtactgc gaatatgtgg aagctgcgga
    19561 tttccccgac ggcatgaagc tgatcgcgga ctttcccaat ctcgtcgtct tccggacctt
    19621 ctccaagatg tacggtctgg gcggcctgcg catcggttac ctggccgggg cgccggaggt
    19681 ggtggatatg atccggcgga cctgcgtggt ctattccgtc aacggcatcg cccaggaggc
    19741 cgccctggcc gccctccgtg acgacgcgga ccatatccgg cggagccggg acctggtgcg
    19801 gagcagccgc gcctatttgc gggaggaact ggggaagatg gacctccccg tcatcaccgg
    19861 cgagggcaac ttcctcgtcg tccggctccc gggcagcgac accctggcct accggagact
    19921 gatgaaagaa ggggtaatga ttcggcccat gacaagcttc cgctatccca atcatatccg
    19981 ggtgaccctg gcccagccgg aggccatgga ggccttcgtg gccgccctga aaaggcttct
    20041 gtccggaaag gtcgccggtt gaacgacgca aactcccctc cccgcttgct gacctacgag
    20101 ttcatcgtct tctgcctgat aaccattacc accttctgca ccgtcagcat tttctacagc
    20161 tttcatcact atctggtgga aatcggcatc cccccgagtt ggcgtggatt aatcatcggc
    20221 atagagccga tgacggcgtt catcctgcgc ctggtcatcc taccctggct ccaggtgcgc
    20281 gacaccccgg cgattatcgc ggcatcgaca gtcgccatga tctttatcgg ctttggatac
    20341 ctctgtgcga ccacggtggc aagcctgatc gtcctgcgca ttttccatgg cgctgccatc
    20401 gtctttctga ccgtggcggt gaccgccctg ggcgtcaact tcatccctcc ggaaaggagc
    20461 ggccaggcct tcgggatctt tcttgtcgtc acgatcgcac cttatgcgtt cattcccccc
    20521 atggcggaag caatcctccc ctatttgcga aacgaagcgg atctctatgc cggggtctcg
    20581 atctttgttt tcttcgccct cctgttgctg gcgcgggggt ggaagcggat cgccagttac
    20641 atcgccggca tggacggagc cttgttgcgc cgccccaccc gggtggagct gtacgataat
    20701 ttctccatcg ccacggtaag aaaaatgttg tggtgctttt cccttgtcta tctgatccac
    20761 gccaccctgt ttttctttct caaggacctg accctggaaa tcaccgtggg ccgggtgggc
    20821 accttctttt ccatattcat gaccatgacg attgccgtac gccttgccgg cagccgttac
    20881 ttcgaccggg tgaacaaaaa gaagctcctt ctggcgggat ttgccgccat ggcgacctgc
    20941 ttcgccgtcc tgcccaccgc cggttccccc ggtctttact atctcctggc gggactctac
    21001 ggactgggga tgggggtgat catgccagtc atgaacgccc ttctcttcgc cgcctcccca
    21061 ccggcgctcc gggggatcaa tgtaaacatg accatgttca cgctggatct gggctatttt
    21121 atcatgcctt acctgggcgg cctcctcatc gccctgggaa tgggtttcag catccttttc
    21181 tatgccggcg ccggtttcgc cgtcctggcc gctgctatcg tcggcaccgt gaaaacgccg
    21241 ggcgattagc ggttgaaatg atgccgggcg ttatgctaat tgtgtcccat gatggttatg
    21301 atggcgcgca aagcaagctt gctatgtctg gccatgctgg ccggtctcct gatcacccag
    21361 gggtgcggca aggggagcta caagaccaag gaaaaacgga tcgtccggaa gatgctcgtc
    21421 accggttacg acgccgggca aaaatccacg ggctggaagc gcaaatgggg ccttttcttc
    21481 atgccccccg tttacgccta cgggcccaac gagggtaagc ggaaggaggt cggggtaacc
    21541 gccgacggca ctcaggctaa aaagggcgtt atcgccgcgg acatcaaccg ttatccctac
    21601 ggcaccaaga tgtacgtccc cggttacggc tggggggagg tgcacgacat cggcaccgcc
    21661 atcaagggcg accatatcga ccttttcttt cccagtgaaa aagaggcaaa aaagtgggga
    21721 aggaaatacc tgaaagtcat catcctgttg agggaataaa tggacttttt ctttaagccg
    21781 caaggcatcg ccgtcaaagg agaggatgga tccctggccg gccatggagc atcatgcccg
    21841 gcgattgctg aaagtatata atttgatcaa tcatcatagc atcctcaccc tatcgtacgt
    21901 ttgcattgac aaggtatttt ttatctggta at
//
LOCUS       DAOSMF010000024        21267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_3072_length_21267_cov_20.667971, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000024 DAOSMF010000000
VERSION     DAOSMF010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 21267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21267
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3072_length_21267_cov_20.667971"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="PLT63_04425"
     CDS             complement(<1..379)
                     /locus_tag="PLT63_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD98004.1"
                     /translation="MEEENTTVSVVKEQYGADSIKVLEGLEAVRKRPAMYIGSTGKEG
                     LHHLVYEVVDNSIDEAGAGYCDLITVRIRLDNSVMVEDNGRGIPVEIHKTEGVSAAEV
                     VLTKLHAGAKFSNDSYKISGGLHG"
     gene            complement(401..1516)
                     /gene="dnaN"
                     /locus_tag="PLT63_04430"
     CDS             complement(401..1516)
                     /gene="dnaN"
                     /locus_tag="PLT63_04430"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOD98005.1"
                     /translation="MEFHIGRNTFLEGVQKTLGIVERKTTIPILNNILIRTEDGRIKI
                     VATDREIGLIARYEARIEVPGDITLSAKKLYEMIRETQGETIHFLKNEQHQVTMRSNK
                     AIYRIPGLPADQYPQVVEADDITLYKVSGAVLADLIGKTAFAVSTDEIRMNLNGIFFD
                     TDGAGDKVRLKMVATDGHRLAEALGPAGSAGGMTLEKGVIIPRKGMLEVRKIAEEEEE
                     VEIGIDHGMFIMKNKNLTIKVSLIDAEYPDYRRVIPAEKGTLLQFSRDKVLHALRRMV
                     VISSERYSGVIFKLADNLMVLNSNNPDVGEANDEIEVSYGGEELSVSYNVHYLIDAIE
                     AVDEEEVGFEINVGMKPGVIRGVGNDDYLCIVMPLKL"
     gene            complement(1670..3031)
                     /gene="dnaA"
                     /locus_tag="PLT63_04435"
     CDS             complement(1670..3031)
                     /gene="dnaA"
                     /locus_tag="PLT63_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOD98006.1"
                     /translation="MDHKWKEVYNIIKENLNEHNFETWIKPLKIMFMDEQNVRFSVPN
                     RFFRDWVMENHYEVIADSFRAATGRSYHVDFVVTQEKKPSEKAILVSGADRENKPPQK
                     SARGKLSTSLNFKYTFERFVIGASNQFAHAAAMAVAEQTEQTAKSYNPLFIYGGVGLG
                     KTHLLNAIGLKALDLAPHLNIVYVSAESFMNELIKCIRDDKMPGFRDKYRNIDCLLMD
                     DIQFIAGKERTQEEFFHTFNTLHDAGRQIVVTSDKFPKEIPNLENRIRNRFEWGLIAD
                     IQPPDIETKVAIIEKKASENNIHLPANVAYYIASNAESNIREMEGFLLRIVAYSSLKR
                     REIDIDLVREVLKHMIRTNTRTELSIDDIIKVVSAKYGVKSSDIKSHKKNKNIVAARQ
                     MCMHLARKFTNCSFPDIGARIGGRDHSTVIYACKKIGMLMEADVKLKNLSEEIEEALN
                     NIH"
     gene            complement(3085..3948)
                     /locus_tag="PLT63_04440"
     CDS             complement(3085..3948)
                     /locus_tag="PLT63_04440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOD98007.1"
                     /translation="MSAKPSLGKGLGAIFPDLLKGLENKPAFIMCGIEELTPNRFQPR
                     KSFPEKEQQELVNSIKKSGIIQPIVVRRSEQGYEIIVGERRWRAAQQAGLKEVPIIIR
                     GAEDQEIAELSLIENLQRESLNPIEEAEAYQILTGRFGLLQEDIAARIGKDRSTVANT
                     LRLLKLPGEVKKALAAKTITSGHARALLSLVDAALQLQALKLILDRDLNVRGTEKLVA
                     HMKKSAAKTTGKTRKKDVYILDMERQLTERLMAAVHIRPGQRSGTIEIRFASGDELNR
                     IFRLLNEIGTA"
     gene            complement(3948..4718)
                     /locus_tag="PLT63_04445"
     CDS             complement(3948..4718)
                     /locus_tag="PLT63_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOD98008.1"
                     /translation="MRKIIGIANQKGGVGKTTTAINLAASLATAERRTLLIDCDSQGN
                     ASTGVGMERSAIRERNIYHGLLGQCSLEEIIRRTSLPFLSVAPADQNLVGAEVEFVTM
                     ANREKRLRRLLDDLTDDYQYIIIDCPPSLGFLTINALAAADSLIVPLQCEYFAMEGLG
                     NLLNTVRLVRQSFNPDLNIGGILLTMFDARNLLSHRVSEDVRKHFGRLVFDTVIPRNV
                     RLSECPSHGLPIILYDIKSRGAAAYMELAREIMQNGRL"
     gene            complement(4848..5567)
                     /gene="rsmG"
                     /locus_tag="PLT63_04450"
     CDS             complement(4848..5567)
                     /gene="rsmG"
                     /locus_tag="PLT63_04450"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOD98009.1"
                     /translation="MEGNLCLHGGDAPEQEPLRALREGATALGVSLEAAELRRFAAYE
                     EELLRWNRLVSLVAVKSPLDIAVKHFLDSLSLLPHLPREEGLRLLDIGTGGGFPGLPL
                     KIVRPEIELHLLEISRKKVSFLREAVRKLGLTGVEVIHERVEAVIRAGLRAGAYDAVV
                     SRAAFPTAELLAMGAHFLRPGGLLLVMRGPRKLEEPAVSQDLYQEKAVSFRLPVRGYP
                     RMIIIFRKAAATLKSALPPAQ"
     gene            complement(5690..6610)
                     /locus_tag="PLT63_04455"
     CDS             complement(5690..6610)
                     /locus_tag="PLT63_04455"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOD98010.1"
                     /translation="MVDLEYLKRINLVSIPRMQQFLGTFMLGPNYNIFQKVDIVLENI
                     ENIPPGENVIFAMNHTDRFNYWPFQYKLWRLKRYNWTTVWVKGKYYRNKWLGKGLDSC
                     NLIPVPSLGYLIEEFYTQKYARRIDRDLYRRVRDAIDGKDEGTEVHAANIAETNRLFG
                     GDFIVFIRDFYERIMAKVADLTRIALFERGLSLIIFPEGTRGTQLGEGKTGIAQVALH
                     TGKTVVPVGCNNSDQVYPDSLPFARTGRIVYRVGKPLSIADRLRPFRIEEPFQLLSRS
                     SQQQYKEQFEGATNVIMESINELLDENYRR"
     gene            complement(6745..7509)
                     /gene="kdsB"
                     /locus_tag="PLT63_04460"
     CDS             complement(6745..7509)
                     /gene="kdsB"
                     /locus_tag="PLT63_04460"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HOD98011.1"
                     /translation="MTFNNFSHKIVCIIPSRYESSRFPGKPLADLLGKPMIQHVYERV
                     KKSELVSVVAVATDDRRIYDAVTAFGGRAVLTAAVHPSGTDRIHEAATLLGLADEDIV
                     VNIQGDQPLFNPIQIEEVARPLLDDPAIPMSTLIYRIVREEEIGHPNAVKTVCDQRGF
                     ALYFSRATVPFVRDRDKTADYYKHHGIYAYRRHFLRTFAALPVGRLERLEALEQLRAL
                     EYGYKIKVVETAHDSVEVDTPAELARVRQLLLQAKK"
     gene            complement(7581..8552)
                     /gene="rfaD"
                     /locus_tag="PLT63_04465"
     CDS             complement(7581..8552)
                     /gene="rfaD"
                     /locus_tag="PLT63_04465"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR02197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HOD98012.1"
                     /translation="MIVVTGGAGFIGSSFIWKLNRQGIEDIVIVDRLGSSEKWKNLVN
                     LRYIEYIHKDDFLQMVYADQVPFTTRTVVHLGACSSTTERDADYLWRNNYLYSCRLAD
                     WCVRSGIRFIYASSAATYGDGARGFSDADDITPTLKPINMYGYSKQVFDLWVLKRGLE
                     KTVAGLKFFNVFGPNEYHKGDMRSVVHKAFGQIKASGRVRLFKSNRPAYADGGQLRDF
                     VYVKDCVEVMWWLLENPGVGGIFNVGTGKARTWNDLMRATFAAMDLPERIEYIDMPPG
                     LEEQYQYFTEARMEKLSAAGCPVAFRSLEDSVRDYVTGHLRQPDPHL"
     gene            complement(8627..9829)
                     /locus_tag="PLT63_04470"
     CDS             complement(8627..9829)
                     /locus_tag="PLT63_04470"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOD98013.1"
                     /translation="MAGFEVFGKEEMKEVMDVMETGILMRYGFDAERKGVFKVRQFEE
                     EFAAACGAGYALGVTSGSAALKVALTALDVGPGDEVIVPAFTFIATYEAVLEVGAIPI
                     MADIDASLNLDPDDIINKMTPYTKAVIPVHMCGAPANIDRIVEVARKSNLLVLEDTAQ
                     AVGASLRGKKLGTFGDMGIFSFDYYKTITTGEGGMVITDREDLYKRADWYHDHGHDHN
                     PKIHRALEAHPLLGFNYRMNELQGALGLAQFRKLPEIIAAQRQNKEALKGILKQVPGL
                     MFRHIPDPDGDTATFLGFHLPDAATTERFQSLLGEEKIDTIFFKKNKWHYVPNWEHLI
                     AWSTANSKKFPFTYPSWRGKVDYSIRMIPQAEDLMGRTLFMAIPIKMAAERLGQLQEG
                     IAKAARRM"
     gene            complement(10022..11179)
                     /locus_tag="PLT63_04475"
     CDS             complement(10022..11179)
                     /locus_tag="PLT63_04475"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOD98014.1"
                     /translation="MDKIFSFTGAKKIVFGRGAFQKLPEHIGEFSASRPLIIMDKQLA
                     ATGLKEQIADLLKNNSIEGQFFDKVDPEPKLALADEGAKLAQKAKCDLVVGIGGGSAM
                     DVAKAIAVVVGNKGKAVDYLGLNKVPGPGLPKIMIPTTAGTGSEVTFTSVFVRPELKK
                     KEGMNSPFLYPDLALLDPFLTVSLPPGPTASTGIDALCHAIESYTSINASPLSELISL
                     EAIALISENLRTAVHDGTNIAAREKMLLGSLYAGLGLANAGVTAVHSLSYPLGGKFGI
                     PHGLANTVLLPHVMNYNLPAAQEKFADIAAAMGEVVENIPLREAAGLAVEAVSVLIAD
                     CGVQTSLEDLGIKEEDFEELAKIALTVARPLANNPRPVTLEDAVAIYEDAF"
     gene            11299..11736
                     /locus_tag="PLT63_04480"
     CDS             11299..11736
                     /locus_tag="PLT63_04480"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOD98015.1"
                     /translation="MILLLFGCKPAVLKQDIPVSTNPMHAKILVNGQPAGQTPGIVTL
                     ERNRDHILTLIKEDYRQEDVVIRKQYQSDRVFLKAVQSGVNAGLFHKNAAMGISSGYG
                     SMSMQPDRTDKAAGGLQVEVYKVENAEPLVGAGCIDDDDRRVK"
     gene            11863..12960
                     /locus_tag="PLT63_04485"
     CDS             11863..12960
                     /locus_tag="PLT63_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98016.1"
                     /translation="MQDFSTLSFHISHEDVDRWQEGIRKADPDIAATLDRLLEAEEKK
                     ARITLSKGRLRERWRDLWRRWKETIAEVGDEDGRYAVHEHHWEETYFDEVSLSYDLET
                     IAGEMLPLIDDVYALDNEPDLFRDALEKIAAAIAAYPEWMGVEYEEGCTLGKNTTRCV
                     LKWSRLAAGEGPEAGIALLEMVQRMMATDKKVYLDGNACVSFFAELPAPCCREIYACL
                     AANAETYELQRAHSIWRRVGDLYEERFNPAKYLETCGRFLAENWKYGPPLIAAAMDSD
                     DYKQAETYLVETFASLLRPGDGKTWQPELSIPRTFLYSYHREHAEIILKLLDTWADVS
                     LRLGNPIRRAARLLRIKPHTFRKRAKEKFKL"
     gene            13064..13291
                     /locus_tag="PLT63_04490"
     CDS             13064..13291
                     /locus_tag="PLT63_04490"
                     /inference="COORDINATES: protein motif:HMM:NF016875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOD98017.1"
                     /translation="MILRFRAIERPARRKLMYLHRTRTLQDFRQPPGNQLEALKGDRD
                     GQYSIRINDQWRICFTWSEGDVCDVEIVDYH"
     gene            13302..13604
                     /locus_tag="PLT63_04495"
     CDS             13302..13604
                     /locus_tag="PLT63_04495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HOD98018.1"
                     /translation="MDADMLLDPIPPGEILSEEFMKPLALSANQLARDIDVPPGRISE
                     IINGKRSITADTALRLGKYFGVSPEIWLNLQTDYDMRVARRTIWPAIEPRIRVRAA"
     gene            13728..13946
                     /locus_tag="PLT63_04500"
     CDS             13728..13946
                     /locus_tag="PLT63_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98019.1"
                     /translation="MDAQYTAIVKQEGDWWIGWIEEVPGVNCQEHTRQELIETLRATL
                     HEAIEFNRQDAIASAGSDYEIERIAVSA"
     gene            14128..14559
                     /locus_tag="PLT63_04505"
     CDS             14128..14559
                     /locus_tag="PLT63_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98020.1"
                     /translation="MSHLTAMDGNVARSFKEGQKSAEEAEERKKWLKAKYEQRLLELD
                     GILTAMKGAPEFEDVEEVVSQEHFDDMVTALAMFHVSENGGDIEETSRRMEYEIWNLP
                     NPYLYMYEMIKKFHPRYAGAEVDDDGDREHGRKNKGKHIIH"
     gene            14612..15001
                     /locus_tag="PLT63_04510"
     CDS             14612..15001
                     /locus_tag="PLT63_04510"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HOD98021.1"
                     /translation="MMILIIGAMTPFSLSPAYGSANSRPTLPTADFKVENAVVKIFAT
                     KRNPDPFRPWTKQPSQEISGSGVVIEGKRILTNAHVVLYASQIEVQANEGSNKISATV
                     EACAPGIDLDFNFKYRGPSHILLKAHL"
     gene            16038..16796
                     /locus_tag="PLT63_04515"
     CDS             16038..16796
                     /locus_tag="PLT63_04515"
                     /inference="COORDINATES: protein motif:HMM:NF015686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HOD98022.1"
                     /translation="MFTWMASPEGWIALGTLAALEIVLGIDNIIFISILVGRLPAEQR
                     GIGRSVGLGLAMLTRLMLLFSIVWVASLTKPWFAVLGQEISGRDIILIGGGIFLLAKA
                     THEIHASLEGIEEAHITCKKATLGMTLIQIALLDVVFSLDSVITAVGLAQHISIMATA
                     IILAVGVMLFAAKPISEFVDGHPTIKILALSFLILVGVTLIVEGFDVHVPKGYIYFSM
                     AFSVTVEMLNLRMRKKQIEPVKLRKELTEIINKT"
     gene            16884..17948
                     /locus_tag="PLT63_04520"
     CDS             16884..17948
                     /locus_tag="PLT63_04520"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOD98023.1"
                     /translation="MKDQDINRFSGIRMRRSVVRLAAVAGGLAVVLWTIWPWLPLAKN
                     RQSRTMVIYGFAIVEKTLTKSIFPAFQKKWKERTGEDLEIISSFGGSGTMTNQLIMGV
                     PAEVAILSTELDMQRLYRSGVLKTESWKRLPYGGVMNRTPFIILVRPGNPKGIRDFDD
                     LAKRGVRIVHPDPLVSGAANWAIMAEYGAALRKPGAKEAQGKAMLLGIWRNVVSQAGS
                     GRAARTQFENGFGDALITYEQDIIATTEHHVSTQEAVYPRSTILSEHTVTPIDKHIKP
                     HQRELIQAFLDFLWTDEAQLLFVQDGFRSVKERLNGANPRIGVITDPFLIDDFGGWGE
                     AKRNIIDRIWKDEVLKELKS"
     gene            17945..18775
                     /locus_tag="PLT63_04525"
     CDS             17945..18775
                     /locus_tag="PLT63_04525"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOD98024.1"
                     /translation="MKSNGMAGQKVTSFLPAGFLLVVMLPLMILPLAAVFIYALQEGP
                     KTFMAALATRDALFALRFSVIIAFATAAVNVVLGTYAAYVLSKTRFPGREQLGIILNL
                     PVAIPNVVVGTSLLLLWGPIGILGKFLEPLGIQPMFTAFGVLLAHIFVTFPYMVGAVK
                     PVLDDLPVTYEEAAYTIGASRRQTFYHVVFPALRGGIITGALLTFAHSLGEFGATVMV
                     SGNLRLQTQTAPLYIFSQFEGGNIAAANSVAAILAVISFFLFSLILYWNRKRESKKAR
                     "
     gene            18814..19899
                     /locus_tag="PLT63_04530"
     CDS             18814..19899
                     /locus_tag="PLT63_04530"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD98025.1"
                     /translation="MIGIEGISKRFGDNEVVKDMSLTVKDGELLVLLGASGSGKSTVL
                     RMVAGLIFPDEGRVLLDGVDITRSSPRERNFGYVFQNYSIFPSMTVAENVEFGLKIRK
                     KPPEERKRQRQRLLEMVGLEELGERYASQLSGGQLQRVALARSLAYNPRVLLLDEPFG
                     ALDAKTRIQLRQAVRNVIRQVNVTTIMVTHDQDEAFEMADRIAILNEGRIDQLGRPSD
                     IYYHPITYFTADFVGEINFFAGTVTRVEREGCEIELAGGIRVFRRGAYPFAPGMPVRY
                     GIRPEQMTISLLEPKSHENGISGIIEKNLFLGDITRYAIRLSDARLLDVRILNYLFMD
                     GMAMPYDLNEEVWLIWSQGSGIILADA"
     gene            complement(19978..20922)
                     /gene="cbiB"
                     /locus_tag="PLT63_04535"
     CDS             complement(19978..20922)
                     /gene="cbiB"
                     /locus_tag="PLT63_04535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HOD98026.1"
                     /translation="MPLGYQIVVAVLLDLLWGDPRRLPHPVKFIGRFALLSETVTRKL
                     FSSPRLAGIVTVLAVLTVTAGLCWGLLLLGRRLYPWLGDTLSILIIYTTLAARDLTRH
                     SGEVYDSLARGDLPGARRRIGMIVGRDTDVLDEQGVTRAAVESVAENTADGVTVPLIF
                     AFLGGPLGAMLYKAVNTMDSTFGYKNERYREFGWAAARLDDLVNYLPARLTAALIPAA
                     ALPLGLNVRNSIKILRRDRRHHPSPNSGWLEAATAGALGVQLGGLNHYFGQPSARPTM
                     GDPLRPLEARDILRVNRLMFATYALILAGGLALIHAAG"
BASE COUNT         4575 a         5795 c         6026 g         4871 t
ORIGIN      
        1 cgccgtggag gccgccggag atcttgtagc tgtcgttgga aaatttcgcc ccggcgtgca
       61 atttcgtcag gactacctcc gccgctgaaa ctccttccgt cttgtggatt tccaccggga
      121 ttccccggcc gttgtcctcc accatgacgc tgttgtccaa tctgatcctg acagtgatca
      181 agtcgcagta accggcaccg gcttcatcaa tactgttatc cacaacctca taaacaaggt
      241 gatgaagccc ctccttgccg gtggagccga tgtacatggc cgggcgtttg cgaaccgctt
      301 ccagtccctc caggaccttg atgctgtccg ccccgtactg ttccttcact accgataccg
      361 ttgtattttc ttcttccatt ttacaaataa tttcctattc ttataacttg aggggcatga
      421 cgatacacag gtaatcgtcg ttgcccactc ccctgatcac cccaggtttc atccccacat
      481 tgatttcaaa acctacctct tcttcatcca ccgcctcgat cgcgtcgatc agataatgga
      541 cattgtagct tacggacagt tcttcaccgc cgtaggagac ctcgatctcg tcgtttgcct
      601 cccctacgtc gggattattc gaattcaaca ccatcaggtt gtcggcaagc ttgaaaatta
      661 ccccgctgta gcgttcgctg gagattacta ccatccttct cagggcgtga agaaccttat
      721 cccggctgaa ttgcagcaat gttcccttct ccgcgggaat cacgcggcgg taatccggat
      781 attcggcatc gataagactg accttgatgg tgagattctt gttcttcatg atgaacatgc
      841 cgtggtcgat gccgatctcc acttcctctt cctcttccgc tattttcctt acctccaaca
      901 tccccttgcg cggaatgatc accccttttt ccaaggtcat tccgccggcg gaaccggccg
      961 gtcccagggc ctccgccagg cggtgtccgt ccgtggccac catcttcagg cggaccttgt
     1021 cgccggcgcc gtcggtatcg aagaagatgc cgttcaagtt catgcggatc tcgtcggtgg
     1081 acacggcaaa agccgtcttg ccgatcagat cggccaacac cgcgccggac accttgtaca
     1141 aggtaatgtc gtccgcctcc accacctggg gatattgatc cgccggcagc cccgggatcc
     1201 ggtaaatcgc cttattgctt ctcatcgtca cctggtgctg ttcatttttc aggaaatgga
     1261 tggtttctcc ctgagtttcc ctaatcattt catatagttt cttcgccgag agggttatgt
     1321 cgccaggaac ctctatccgg gcttcatacc gggcaataag gcctatctcc cggtccgtgg
     1381 ccacgatctt gatccgcccg tcctccgtac ggataaggat attgttcaaa atcgggatgg
     1441 tcgttttccg ttccacgatt ccaagggtct tttgcactcc ttccaggaag gtatttctgc
     1501 caatgtgaaa ttccatcctt gactcctttc ctcttttatt tattttttta tatctttaaa
     1561 aaacagaacc agtagtaaga cctgtccaaa aggggtaaga aaacaaaaaa ctcttttttt
     1621 ccgcctttct cagtgatttc tatctgtcga taacttcttg acgaccgcgt taatggatgt
     1681 tgttcaatgc ctcttcgatt tcctcactga ggtttttcag cttcacatcg gcctccatca
     1741 acatgcctat ttttttgcag gcgtagatca ccgtggaatg gtcgcgaccg ccgatccgtg
     1801 cgccgatgtc gggaaaggag caattggtga actttctggc caggtgcatg cacatctgcc
     1861 ttgccgccac aatgtttttg tttttcttgt gcgatttgat atcggagctc ttcacgccgt
     1921 atttggcgct caccaccttg atgatgtcgt cgatggataa ctccgttcgg gtgttggtgc
     1981 ggatcatgtg cttgaggact tcccgtacca ggtcgatgtc tatttcccgg cgtttgagag
     2041 aggagtaggc tacgatcctc aggaggaatc cctccatctc ccggatattc gactcagcgt
     2101 tggaggcgat ataataagcc acgtttgccg gcaggtggat gttgttttcg ctggccttct
     2161 tttcgatgat ggcaaccttg gtctcgatgt ccggcggctg gatgtcggcg atgagacccc
     2221 attcgaagcg attgcggatc cggttttcca gattgggtat ttccttggga aacttgtcgc
     2281 tggtgacgac gatctgcctg cccgcatcgt ggagggtgtt gaaggtatgg aaaaattcct
     2341 cctgggtcct ttccttgccg gcgatgaact ggatgtcgtc catgagcagg cagtcgatat
     2401 tgcgatactt gtcccggaaa cccggcatct tgtcgtcacg gatgcatttg atcagctcgt
     2461 tcatgaagga ttctgcggag acataaacga tgttcagatg gggggcgaga tcgagggcct
     2521 tgaggccgat ggcattgaga agatgggtct ttcccagtcc gacccccccg tagatgaaca
     2581 ggggattata gctcttggcg gtctgttccg tctgctccgc cacggccatg gcagcggcgt
     2641 gggcaaactg attcgaggcg ccgatgacga aacgctcgaa ggtgtattta aaattgaggg
     2701 aggtcgaaag cttaccgcgg gcgctttttt ggggcggctt gttctcccgg tccgcgccgg
     2761 aaacgagaat ggccttttcc gagggtttct tttcctgggt gacgacaaaa tcgacatggt
     2821 acgaacgtcc cgtcgccgcc cggaaggaat cggcgatcac ctcgtagtgg ttttccatca
     2881 cccagtcccg aaagaaacga ttgggtacgg aaaagcggac gttctgttca tccatgaaca
     2941 taatcttgag gggttttatc caggtttcga aattatgttc attaagattt tctttaatta
     3001 tattatatac ttctttccac ttatgatcca aatttcctgc cccttgtcaa caactttgta
     3061 aacacatgtt gatgatttac gtttctatgc cgtacctatt tcgttaagca atctgaagat
     3121 acgattcaat tcgtctccag aggcgaaacg gatctcgatg gttcccgatc tctggcccgg
     3181 ccggatatgc accgccgcca tgagccgctc ggtgagctgt ctttccatat cgaggatata
     3241 gacgtctttt tttctcgtct ttcccgtcgt tttcgccgcg gatttcttca tgtgggccac
     3301 gagcttttcc gtcccccgga cattgaggtc tctatccagg atcaacttca gggcctgcaa
     3361 ctggagagcg gcgtccacca gagaaagaag ggcccgggca tggccgctcg tgatcgtctt
     3421 ggcagcgagg gcctttttca cctcccccgg cagcttcaac aaccgcaggg tattggccac
     3481 ggtggagcgg tccttgccga tccgggcggc gatatcctcc tggaggaggc cgaatcggcc
     3541 ggtaaggatc tggtaggcct ccgcttcttc gatgggattc agggattccc gctggagatt
     3601 ctcgatgagg gacaattccg ctatttcctg atcctcggcg ccgcgaatga tgatcggcac
     3661 ctctttaaga ccggcctgct gggcggcccg ccatctcctt tcgccaacga tgatctcgta
     3721 accttgttcg ctcctccgga cgactatcgg ctggattatg ccacttttct taatggaatt
     3781 gacaagttcc tgttgctctt tctccggaaa ggacttgcgg ggctggaaac ggttcggggt
     3841 gagttcctcg attccgcaca tgatgaaggc gggtttgttc tccagccctt tgagaagatc
     3901 ggggaagatc gccccgagcc ctttgccgag ggacggtttt gcggacatta cagcctcccg
     3961 ttctgcatga tttcccgggc cagctccata taggcggcgg cgccccggga tttgatatcg
     4021 taaaggataa tggggagtcc gtggctcgga cactccgaca acctcacatt ccggggaatg
     4081 acggtatcga agaccaggcg gccgaagtgc ttccgcacgt cctcgcttac ccggtgcgac
     4141 agcaggttgc gggcgtcgaa catggtcagg agtatccccc cgatattcag gtccgggttg
     4201 aagctctggc ggacgagccg gacggtgttg aggagattgc cgaggccctc catggcgaag
     4261 tattcacact ggagcgggac aatgagactg tccgccgccg ccagggcatt gatggtcaaa
     4321 aagcccaagg acgggggaca atcgattata atatattgat aatcatcagt taaatcatca
     4381 aggaggcgtc gcagccgctt ctcccggttg gccatggtca cgaattccac ttccgccccg
     4441 acaaggtttt ggtccgccgg cgccacgctg agaaagggga gagaggtcct gcggatgatc
     4501 tcctccaagg aacactggcc aagcaggccg tggtagatgt tccgttcccg gatggcgctc
     4561 ctttccatcc cgactccggt ggaggcgttg ccctgggagt cgcaatcgat aagaagggtg
     4621 cgccgctccg ccgtggccag ggaagcggct agattgatgg ccgtggtggt ctttcccacc
     4681 ccgcctttct gattggcgat gccgatgatt ttacgcatta ttttactaag ttttttccag
     4741 gacttatatc ttgacttatt gaagcgaaga ctaccacaaa acgaactatt ttggaagcca
     4801 ttttttagaa aaaagcataa tttaaattgt tgtaatgatt gaattattta ttgagcgggc
     4861 gggagggcgg attttagcgt cgctgcggcc ttccgaaaga tgattatcat ccggggatag
     4921 ccccggacgg gaaggcgaaa cgacacggct ttttcttggt atagatcttg agatacggcc
     4981 ggttcctcca gttttcgggg cccgcgcatg accagcagca gacctccggg acggagaaaa
     5041 tgggcgccca tcgccaggag ctccgccgtc ggaaaggcgg cccgggacac cacggcatcg
     5101 taggctcccg cccgcaggcc cgcccgtatc accgcctcca cccgctcgtg gatgacttct
     5161 accccggtga ggccgagttt acggactgcc tcccggagaa aagaaacctt ttttcttgat
     5221 atttccagga gatgtagctc aatttccgga cggacgatct tcaggggcag gcccggaaaa
     5281 cctccccccg tgccgatgtc aaggaggcgc aggccctcct ctctggggag atgaggaagg
     5341 agggagaggg aatcgaggaa atgtttgacg gctatatcca gcggcgattt caccgccacg
     5401 aggctgacca ggcggttcca ccggagcagc tcttcctcat aggcggcgaa acggcgcagt
     5461 tccgccgctt cgagagagac gcccagtgcc gtcgctcctt cccggagcgc ccggaggggc
     5521 tcttgctcgg gggcgtcacc gccatgcagg cacaagtttc cttccaccta aaaattcgtt
     5581 cctatatgcc ttacttaatt cgctaatcat cttcctcccc ttcaatggga ggaccaaggt
     5641 ggggatgggt tttgtgtgaa aacgcccgcg gatggacccg cttcacctct cagcgccggt
     5701 agttctcatc gaggagctca ttgatgcttt ccatgatgac gttcgttgcc ccttcaaact
     5761 gctctttata ctgctgctgg gaagaccggg acagaagctg gaaaggctcc tcgatgcgga
     5821 atggccggag gcggtcggca atggaaaggg gcttcccgac gcgatagacg atccggcccg
     5881 tgcgggcgaa gggaaggctg tcggggtata cctgatcgga attgttgcag ccgacgggca
     5941 cgaccgtttt cccggtgtgg agggctacct gggcgatccc cgtcttcccc tctcccaact
     6001 gggttccccg ggttccctcg gggaagatga tgagactcag cccccgttcg aaaagggcga
     6061 tccgggtcaa atccgctacc ttcgccatga tccgttcgta aaagtcccgg atgaacacga
     6121 tgaagtcccc gccgaaaaga cggttcgttt cggcgatgtt ggcggcatgg acctccgtcc
     6181 cctcgtcttt gccgtcaatg gcgtctcgga cccgtcggta gagatcccgg tcaatccgcc
     6241 gggcgtattt ttgggtataa aattcttcga tcaaatatcc cagggagggc acagggatga
     6301 ggttgcacga atccagtccc ttgccgagcc acttgttccg gtaatacttg cccttcaccc
     6361 agaccgtcgt ccagttgtac cttttcagcc gccatagctt atactggaaa ggccagtagt
     6421 tgaaccggtc ggtgtggttc atggcaaaga tgacgttttc gcccgggggg atattttcga
     6481 tattttccag aacaatatca actttttgga agatattgta atttggtccc agcatgaacg
     6541 ttcccagaaa ctgttgcatt ctcgggatgg aaaccaagtt gattcttttc aggtattcaa
     6601 ggtcgaccat aatttaagaa aacctctatg atttcttgag ttatcaaaac tcctgccctc
     6661 aaaagttgac gattcggcaa taagtctcaa aaaccatccc ctttaaaaag agggctgagt
     6721 ggggatggtt ttttgcgccc ggcttcattt ttttgcctga agcagtagtt gacggacccg
     6781 ggccagttcc gccggggtgt ccacctcgac ggaatcgtga gcggtttcga caacctttat
     6841 cttgtagccg tattccaggg cccggagctg ttccagggcc tccagacgtt ccagccttcc
     6901 caccggcagt gccgcgaagg tccggaggaa atggcgccgg taggcgtaga tcccgtggtg
     6961 tttgtaatag tccgccgttt tgtcccggtc ccggacaaag gggaccgtgg cccgggaaaa
     7021 atagagggcg aagccccgtt gatcgcagac cgttttcacg gcgttcgggt ggccgatttc
     7081 ctcctcccgg acgatgcgat agatgagggt ggacatgggg atggccgggt catccaggag
     7141 gggccgcgcc acctcctcga tctggatggg gttgaagagg ggctgatccc cctggatgtt
     7201 cacgacgatg tcctcatcgg ccagccccag gagcgtggcc gcctcgtgaa tccggtccgt
     7261 tcccgagggg tggaccgccg ccgtcaatac ggcccggccg ccgaaggccg tgacggcatc
     7321 gtatatccgc cggtcgtccg tggccacggc aacgacggaa accagctccg actttttgac
     7381 ccgctcgtaa acatgctgga tcatgggctt acccaggaga tccgccaggg gcttgccggg
     7441 gaaccgcgac gattcgtacc gggaggggat gatacagacg atcttgtgag agaagttgtt
     7501 aaaagtcatt aggcgtattt atggtaattt tctctgggcg gttcggtaat gttagaaaat
     7561 tggggattat ccgtcaattg ttaaagatgc gggtcgggct gccgcaggtg gcccgtcaca
     7621 taatcccgca cagagtcttc cagggagcga aaggccacgg ggcagcccgc ggcggacagc
     7681 ttttccatcc gcgcctcggt aaaatactgg tactgttctt ccagcccggg gggcatgtcg
     7741 atgtattcga tccgctccgg caggtccatg gccgcgaagg tggcccgcat caggtcgttc
     7801 caggtccggg ccttgccggt gcccacattg aagatcccgc ccacgcccgg gttttccaag
     7861 agccaccaca tcacctccac gcagtccttt acataaacga aatcgcgtag ttgaccgcca
     7921 tcggcatagg cggggcggtt ggacttgaag agccggaccc tccccgaggc cttgatctga
     7981 ccgaaggcct tgtggacgac gctccgcatg tcgcccttgt ggtactcgtt ggggccgaag
     8041 acattgaaga atttcagccc ggcgacggtc ttttccaggc cccgcttcag gacccagagg
     8101 tcgaagacct gcttggaata gccgtacata ttgatgggct tcaaggtggg ggtgatgtcg
     8161 tcggcgtcgg agaagccccg ggcgccgtcc ccgtaagtgg cggcggagct ggcgtagatg
     8221 aaccggatgc cgctccggac gcaccagtca gcgaggcggc aggagtagag atagttgttc
     8281 cgccagagat aatcggcgtc ccgttccgtg gtggaggagc aggcccccag atgaacgacc
     8341 gtccgggtgg tgaaggggac ctgatcggca tagaccatct ggaggaagtc gtctttgtgg
     8401 atgtattcga tataacgcag gttgacgaga ttcttccact tttccgaaga acccagccta
     8461 tcgacgatga cgatgtcttc gattccctgc cggttgagct tccatatgaa ggagctgccg
     8521 ataaatccgg cgccgccggt gaccacgatc atgcctgcat ctcctctgac gctgtttcta
     8581 ataaagacct tataacgctc atgacggcag gccgtcaccc ctcccgtcac atcctcctcg
     8641 ccgctttcgc gattccctcc tgaagctgcc ccagcctttc cgctgccatc ttgatgggga
     8701 tggccatgaa cagggtccgt cccatcaagt cctccgcttg ggggatcatc cggatgctgt
     8761 aatccacctt cccccgccag gacggatagg tgaaggggaa cttcttggaa ttcgccgtac
     8821 tccaggcgat gagatgctcc cagttcggta cgtagtgcca cttgtttttc ttgaagaaga
     8881 tcgtgtcgat tttctcttct cccagcaggg actggaatct ctccgtagtg gcggcgtcgg
     8941 gaagatggaa ccccaggaag gtggcggtgt cgccgtccgg atcgggaatg tggcggaaca
     9001 tcaagcccgg gacctgcttc aggatgccct tgagggcctc cttgttttgc cgctgcgcgg
     9061 cgatgatctc cgggagcttc cggaactggg ccagccccag ggccccctgg agttcgttca
     9121 tccggtagtt gaagcccagc aggggatggg cctccagggc ccggtggatc ttgggattgt
     9181 gatcgtggcc gtgatcgtga taccagtcgg cccgcttgta gaggtcctcc cggtcggtga
     9241 tgaccatgcc gccctcgccg gtggtgatgg tcttgtagta gtcgaagctg aagatgccca
     9301 tatccccgaa agtgccgagc tttttccccc gtaaggaggc cccgacggct tgggcggtgt
     9361 cctccaggac caggagattg gatttccggg ccacctcgac gatccggtcg atattcgccg
     9421 gggcgccgca catgtggacg gggatgaccg ccttggtgta aggggtcatt ttattgatga
     9481 tgtcgtcggg atcgaggttc agggaggcgt cgatgtctgc catgatcgga atggccccca
     9541 cctcgaggac cgcctcgtag gtggcgatga aggtgaaggc cgggacgatg acctcgtcgc
     9601 cgggacccac gtcgagggcc gtaagggcca ccttgagggc cgccgacccg gaggtgactc
     9661 ccagggcata tccggcgccg caagcagcgg cgaattcctc ctcgaactgc cggaccttga
     9721 agactccctt ccgctccgca tcgaatccat agcgcatcag gatgcccgtc tccatgacgt
     9781 ccatgacttc tttcatttct tccttgccga atacctcaaa accggccata gcgacctgtc
     9841 ctccttagta taatatcttg tggttaatta ccgcttcact aaccctcatc ccgttacggg
     9901 tacaacgaaa gatgaaaata tccggctact tgttgccatt ttaatataaa tgtagtgcgt
     9961 agctttagct gcgctgttgg cggacctgaa ggtccacact acattctcag gtgaatgttt
    10021 ctcaaaaagc gtcctcgtag attgccaccg cgtcttccag ggtcaccggc cgggggttgt
    10081 tcgccagggg ccgggcgacg gtgagggcga tcttcgccaa ctcctcgaaa tcctcctcct
    10141 tgatccccag gtcttccagg ctcgtctgga cgccgcagtc ggcgatcagg acgctgactg
    10201 cctccacggc caagccggcc gcctcccgga ggggtatgtt ttccactact tcccccatcg
    10261 ccgcggcaat atcggcgaat ttctcctgcg ccgcggggag gttgtagttc atgacatggg
    10321 ggagcaggac ggtattggcc aggccgtggg gaatgccgaa ttttccgccc agtgggtagg
    10381 acaatgaatg gacggcggtg acaccggcgt tggccagtcc cagcccggca tagagactcc
    10441 ccaggagcat tttttcccgg gcggcgatat tggtcccgtc atggacggcg gtccggagat
    10501 tctcggagat caatgcaatg gcctccaggg agattagctc gcttagcggc gaggcattga
    10561 tggaggtgta agactcgatg gcatggcaca gggcgtcgat acccgtggat gccgtcggac
    10621 cgggaggcag gcttaccgtc aggaacgggt ccaggagcgc caggtcggga tagaggaagg
    10681 ggctgttcat cccctctttc ttcttcagct cggggcggac gaaaacagac gtgaaggtga
    10741 cttcgctccc cgtgccggcg gtggtgggga tcatgatctt cgggaggccc ggtccgggga
    10801 ccttattcaa ccccaggtaa tccacggcct tgcccttgtt gcccaccacg acggcgatgg
    10861 ccttggcgac gtccatggcg ctgccgccgc cgatgcccac cacgaggtca cacttggcct
    10921 tttgggccag cttcgcacct tcgtcagcca gggccagctt gggctccgga tcgaccttat
    10981 cgaaaaactg cccttcaatg ctgttattct tcaataaatc ggctatttgc tcttttagcc
    11041 ccgtggcggc caactgcttg tccatgatga tcaggggacg ggaagcagaa aattccccga
    11101 tgtgctccgg gagcttttga aaggcccccc ggccgaaaac gatctttttc gccccggtga
    11161 aggaaaagat tttatccatg cgcgcaccct cgttttgagt tcagacggca gccggcgaac
    11221 cggctgcgcc cgtagccgtg catcatacat aatagtgcgt gatagtcaaa tgaaaatagc
    11281 ttcctctgtg cattcagcat gatcctgttg ctgttcggct gcaagccggc ggtcctcaag
    11341 caggacattc ccgtgtccac caatcccatg catgccaaga tcctcgtgaa cggacaaccg
    11401 gcgggacaga cacccgggat cgtgaccctg gaaaggaacc gggatcatat cctgacactg
    11461 atcaaggagg actaccggca ggaagatgtg gttatccgga agcagtatca atcggatcgg
    11521 gtctttctca aggcggtgca atcaggcgtg aacgcggggt tgtttcacaa gaacgccgcc
    11581 atgggcatca gcagtggcta cggctccatg tcgatgcagc cggaccggac ggataaggcc
    11641 gccggcggtc tccaggtgga ggtgtacaag gtcgaaaatg ccgagcccct ggtaggcgcc
    11701 ggctgcatcg acgacgacga ccgacgggtc aaatgacgtt ggggaccaac ggcaaaaagc
    11761 atcaccacgt ttcaacccca ttattaaaag gcgcattatc gatgaagaag aataccgtca
    11821 atatcggcag ccgtctgctg gaagccatta cgaaggaaca ggttgcaaga cttctcgaca
    11881 ttgtcttttc acatttcaca tgaggacgtt gatcgctggc aggaaggcat caggaaggcc
    11941 gatccggaca ttgcggccac cctcgaccgc ctgctggagg ctgaagaaaa gaaggcaaga
    12001 atcactctgt cgaaagggcg tctccgggag cgttggcggg acctgtggag gcgctggaag
    12061 gagaccatag cggaggtcgg tgatgaagac ggccgctatg ccgtccatga acaccactgg
    12121 gaagagacct atttcgatga agtgagcctc tcatatgacc tggagaccat agcgggggaa
    12181 atgttgccgc tgattgacga tgtctatgcc ctcgacaatg aaccggacct gttccgcgat
    12241 gccctggaga agatagcggc agccatcgcc gcatatcccg aatggatggg ggtcgaatat
    12301 gaagaaggat gtacgctggg gaagaacacc acccggtgtg tcctcaaatg gagccgtctg
    12361 gctgccgggg aagggccgga agccgggatt gccttgctgg agatggtcca gcgcatgatg
    12421 gcaactgata aaaaggtgta tctggacggt aatgcctgcg tgtctttttt tgctgaattg
    12481 cctgccccct gctgccggga gatctacgcc tgcctggctg cgaacgcgga aacgtatgaa
    12541 ttacaaaggg ctcattcgat atggcgtcgc gtcggcgatt tatatgaaga aagattcaat
    12601 cccgcgaaat atctggaaac ttgcgggcgg ttcctggcgg aaaactggaa atatggtccg
    12661 ccgttgattg ccgcggcgat ggatagtgat gattataagc aagccgagac atatctcgtg
    12721 gaaacctttg cctcccttct ccgtcctgga gacggaaaga catggcagcc ggaactatcc
    12781 atcccgagaa ccttcttata ttcctatcat cgcgaacatg cggaaatcat attgaaatta
    12841 ctcgatacat gggcggatgt ctccctgcgc ctgggtaacc cgatccggcg cgccgcccgc
    12901 ctcctccgga tcaagcccca caccttccgc aaacgggcga aggagaagtt taagttgtaa
    12961 cgcaacgtaa aatattcttt acatgttacg catctcgtaa tataaggaaa ccgtgataaa
    13021 atcttttcgc tgcaaagata cggcatcgct ttttgaagat cggatgatcc tgcgttttcg
    13081 tgctattgaa cgaccagccc gtcgtaagct gatgtatttg catcgcactc gcacccttca
    13141 ggattttcgg caaccgccag gaaatcaact ggaggcgtta aagggagata gggatgggca
    13201 atacagtatc cggattaacg atcaatggcg gatttgtttt acctggtcag aaggcgatgt
    13261 ctgcgacgta gaaatcgtcg attaccatta ggagaaaaat tatggatgca gacatgctat
    13321 tggaccccat cccgccggga gagatcctca gtgaagagtt tatgaaacca ttggctttaa
    13381 gtgccaacca actggcacgg gatattgatg tgccgccggg gagaatcagc gaaatcatca
    13441 acggcaaacg atcgataacc gctgataccg ctctgcgcct cggcaaatat tttggcgttt
    13501 cgccggaaat ctggctgaat ctgcagacgg attatgacat gcgggttgcc cgccggacaa
    13561 tctggcctgc catcgagccc cgcatcagag tgcgcgcggc gtaaaactga gacgatgatc
    13621 aaaacaatca gcgcacgaac attaaaagaa ctaagcgctt acataaatac gccgccggtt
    13681 gttgaagaag tatcagccat aggttaagct tgaaggagga acattgaatg gatgcccaat
    13741 atactgccat agtaaaacag gaaggggatt ggtggatagg ttggatagag gaggttccgg
    13801 gcgtaaactg ccaggaacac acccgacagg aacttatcga gacacttcgt gcaaccctcc
    13861 atgaggccat cgaatttaac cgccaagatg cgattgcctc cgccgggagc gattatgaaa
    13921 tagagagaat tgccgtctcc gcatgaaaag agaaaaattg ttgtagtacc ttcgaacgtg
    13981 ttggtgcgaa ttcatcagag aaggtcatag gcattcttgg tggggtaata ccaacctcaa
    14041 caaacgctca tctgtgccaa gacataatga aattgacgac ggccttgcaa gaaaaatatg
    14101 caaggatctt gggataaaac caataaaatg agccatttga cagcaatgga tggaaacgtg
    14161 gcaaggtcgt ttaaggaggg gcaaaaatca gcggaggaag cggaggaacg gaaaaaatgg
    14221 ttgaaggcca aatacgagca gcgtctattg gaactcgacg ggatactcac ggcgatgaag
    14281 ggcgctcccg agtttgagga tgttgaggaa gttgtctcgc aagaacactt tgacgacatg
    14341 gtgacagcgt tggctatgtt tcacgtatcg gaaaatggcg gtgatattga ggagacatcc
    14401 cggaggatgg aatatgaaat ttggaatctg ccgaatccgt acctttatat gtatgaaatg
    14461 atcaagaaat tccatccacg ttatgcgggg gccgaagttg atgacgacgg cgacagggag
    14521 catggcagga aaaataaggg caaacatata attcattagc tctgaaattg aaagaaggtc
    14581 gttccaatct attccctctg ccatcatcgc catgatgatt ctcattatcg gtgcaatgac
    14641 gccattcagc ctcagtcccg cttacggatc ggcgaattcc cgtcctaccc tcccaactgc
    14701 ggatttcaag gttgaaaacg ccgtcgtcaa gatctttgct actaagcgca atcctgatcc
    14761 cttcaggccc tggaccaaac aaccttctca agagataagc ggcagcggtg tggtgatcga
    14821 agggaagcga atccttacga acgcgcacgt ggtgctctac gcaagtcaaa tagaggttca
    14881 ggctaatgag gggagcaaca agatttccgc cactgttgag gcatgcgcac cgggaatcga
    14941 tcttgacttt aatttcaaat atcgaggtcc atctcacatc ctactgaagg cgcacctata
    15001 gcagcggggg aaacgtacat tatgaacgct ttggattcgc aggaagttat ggagccgttg
    15061 cgggaagcct gtcagggtat gtttatgggg aatgcttcgg catgccgccg gggctccgcc
    15121 ggtgcaccgg cgggggtaaa tatatgatat gcaaaaggaa ttgttagcat ggcgccctgt
    15181 tttccgtcgt tgcgccgggg tggcgagagc ggttgtaacc ggcctcaacc ggggagggag
    15241 gtcgggggtc ggaaaaggcc gctccgggac gacctcgggg gcggcggcgg gtgtttttgg
    15301 gagagtgacg agaatcgacg ctcaggaaca ttatgatatt aaaaaggaaa caaccgaccc
    15361 ccgaaaaatt ttacccccga aaaaatgacc gccggtcatt tgcccgattg tagagaggac
    15421 ctgcgggcct tcgggtggag aaggggagat cgccggcctg atcctcgatg tctggctccg
    15481 ctccggggcc gaggaggagc gacgagccaa cttcggtcgc ctgggggatg agatcccggc
    15541 ggcaaccatt cgagctggag ggcgccgccg ggggtgagaa aggcggcaag ttcctgaccc
    15601 gccgcccaca agtccagcgt ttctctactc gtccctccct tacgcgccat cgtcgtgtct
    15661 tatgggcctc cctaaatttt tggggatttt tcgtctatag tcttgatgac cttggcggaa
    15721 ggaagttaaa atgattcact cccggtttgt caatgattat ttccgcccgc ttcccggatc
    15781 gtgtcgcatg aacgcctaat tttgttgaca ttaaaaatat tatgatataa aaaaacccac
    15841 cccaaaaatt atgactccta tgcggagtaa attatgagcg caaggtcttt cgcgtcgtcg
    15901 gagccccagc cggcgagatg gtaaatcctg aattaaatga gaggtgttgt cgatgaagat
    15961 gcggagaatc gtcccgatct tgatactggt tcttgtcctg tcaccggcgg gcgtcattgc
    16021 cgcataggag gtcgattatg tttacctgga tggccagtcc ggaaggatgg attgccctgg
    16081 ggaccctggc ggccctggag attgtgctcg gaatcgacaa cattatcttc atttccatcc
    16141 tcgtggggcg tttgccggcg gagcagcgcg gcatcgggcg cagcgtgggg ctgggcctgg
    16201 ccatgctcac ccgcctgatg cttttgtttt ccatcgtctg ggtggccagc ttgaccaagc
    16261 cctggtttgc cgtcctgggc caggagattt ccgggcggga catcatcctg atcggcggcg
    16321 ggatttttct cctcgccaag gcgacccatg aaatccacgc cagcctcgaa gggatcgagg
    16381 aagcgcatat tacctgtaaa aaggctactt tgggaatgac cctcattcag atcgccctcc
    16441 tcgacgtggt gttctccctc gattcggtga tcaccgccgt ggggctggcc cagcacatct
    16501 ccatcatggc cacggcgata atcctcgccg tgggggtcat gctctttgcc gccaagccga
    16561 tttcggaatt tgtggacggt catcccacca tcaagatcct cgccctgtct tttctcatcc
    16621 ttgtgggcgt caccctgatc gtcgaaggtt tcgatgtcca tgtcccgaag ggatacatat
    16681 acttttccat ggccttttcc gtgacggtgg aaatgctcaa cctgcggatg cgcaaaaagc
    16741 agatcgagcc ggtgaaattg cgaaaagagc tgacggagat aatcaataag acttgaaaag
    16801 gacgaaagat cccaacaccg gcattggcat gaccgcaatg agatgccgcg tgacttcact
    16861 cgaggtcatc agcggttaat atcatgaaag atcaagatat taaccgtttt tccggcataa
    16921 ggatgcgccg gtcggtcgtg cgtctggcgg cggtcgcagg cggcctcgcc gttgtgctgt
    16981 ggactatctg gccctggctg ccgttggcaa agaaccggca atcccggacg atggtcatct
    17041 acggcttcgc cattgtagag aaaaccctta ctaaaagcat cttccccgcc tttcaaaaaa
    17101 agtggaaaga gaggaccggc gaggatctgg agattatcag ttccttcggt ggttcgggaa
    17161 cgatgaccaa tcagttgatt atgggcgtcc ccgcggaagt ggctatcctg tccaccgaat
    17221 tggatatgca acgcctctac cggagcggag tcctcaagac agagtcatgg aagcgacttc
    17281 cctatggcgg cgtgatgaac cgcactcctt tcatcattct cgtacgtccc ggcaatccta
    17341 aagggatccg tgattttgac gatctcgcca aaagaggggt ccggatcgtc caccccgacc
    17401 ccctcgtctc gggggccgct aattgggcca tcatggccga atatggcgcc gccttgcgga
    17461 aaccgggggc caaggaggca cagggcaaag ccatgctgct gggcatctgg cgcaacgtgg
    17521 tctcccaggc ggggagcggc cgggccgctc gcactcagtt tgaaaacggc ttcggcgacg
    17581 ccttgataac ctatgaacag gacatcatcg ccacgaccga acatcatgtt tcaacccagg
    17641 aagcggttta tccccggagc acgatcctga gcgagcatac cgtcacgccc attgacaagc
    17701 acatcaaacc tcatcaacgg gaactgatcc aggccttcct ggacttcctc tggaccgatg
    17761 aggcccagct tctcttcgtg caagacggat ttcgcagcgt aaaagaacga ctcaacgggg
    17821 ccaatccaag aatcggagtc attaccgatc cttttcttat cgatgatttc gggggttggg
    17881 gagaggcgaa acgcaacatc atcgacagga tatggaaaga cgaggttctc aaggagttga
    17941 aatcatgaaa agcaacggca tggccggcca gaaggtcacg tcctttctcc ccgcgggttt
    18001 cctgttggtg gtgatgctgc ccctgatgat cctgcccctg gcggcggttt tcatctatgc
    18061 cctccaggaa ggcccaaaaa cattcatggc ggccctggca acccgggatg ccctctttgc
    18121 actccgtttc agcgtgatta tcgccttcgc tacggcggcg gtcaatgtcg tcctgggaac
    18181 ctacgcggcc tatgtcttaa gcaagacccg tttcccgggg cgggaacaac tgggtatcat
    18241 cctcaacctc cccgtggcca ttcctaacgt ggttgtcggc acatcacttc tgctgctctg
    18301 gggacccatc ggcatcctgg gaaagttcct ggaacccctg ggcattcaac ccatgtttac
    18361 cgccttcggc gttcttttgg ctcacatctt cgtcactttt ccctacatgg tgggcgccgt
    18421 caagccggtc ctggacgacc ttcccgtcac atacgaagaa gcggcataca ccataggcgc
    18481 ttcccggcgg caaacctttt accatgtggt cttccccgcc ttgcgcggcg ggatcatcac
    18541 cggcgctctg ctcacctttg cccatagcct gggggaattc ggcgccaccg taatggtttc
    18601 gggaaatctg cgcctgcaaa cgcaaacggc gcccctttac atcttctctc aattcgaggg
    18661 gggcaatatc gccgctgcca actctgtcgc cgcgattctg gccgtcattt ccttttttct
    18721 cttttccctg atcctctatt ggaacagaaa acgggaaagc aaaaaggctc gataatgata
    18781 ctgttttttc tttagaggag atacgcggac catatgatcg gcattgaagg aatcagcaaa
    18841 aggtttggcg acaacgaggt cgtcaaggat atgtctctga cggtaaagga tggagaactg
    18901 ctcgttcttc tgggggccag cggcagcggc aagagtactg tcctgcgcat ggtggcgggg
    18961 ttgatatttc ccgatgaagg gagggttctt ctcgatggcg tggatatcac ccggtccagc
    19021 ccgcgggagc gaaattttgg ctatgtattt cagaattact cgattttccc ctccatgacc
    19081 gtggcggaaa acgtggaatt tgggttgaag ataagaaaaa aacctcccga ggaacggaaa
    19141 aggcagcggc aacgccttct cgagatggtg ggcttggaag aactcggaga acgctacgcc
    19201 tcacagcttt ccggaggtca attacagcgg gtggccctgg cccgatccct ggcatacaat
    19261 ccccgggttc tcctcctgga tgaacccttt ggcgccctgg acgccaaaac caggattcag
    19321 ctccgccagg ccgtgcggaa tgtcatccgg caagtgaacg ttaccaccat catggtcacc
    19381 catgaccagg acgaggcttt cgaaatggcg gaccgcatcg ccattctcaa tgagggacgc
    19441 atcgaccagt tgggccgtcc cagtgacatt tactaccatc ccattacata ctttaccgcc
    19501 gattttgtcg gagaaatcaa tttctttgcc ggaacggtca cgagggtgga aagggagggc
    19561 tgcgaaatcg aactggccgg tggcatccgt gtctttcgtc gcggcgccta tcccttcgca
    19621 ccgggaatgc cggtgcgtta cggcatccgt ccggaacaaa tgacgatcag ccttctcgaa
    19681 ccgaaatcac acgaaaacgg catctccggc attatcgaga aaaacctttt tttaggtgac
    19741 atcacccggt atgccattcg tctttccgac gcccgtcttc tcgatgtgcg catcttgaac
    19801 tacctgttca tggatggcat ggccatgcct tacgatctta atgaagaagt ctggcttatc
    19861 tggagccagg gttccggaat catccttgct gacgcctgaa cggacaacca tcggaaagaa
    19921 tgtggaaata ttagtaatat caatttgtag ccagcggata tgcggtccct tgcgtgttca
    19981 acccgcggcg tggatcaggg ccaatccccc agctaagatc agggcgtagg tggcgaacat
    20041 cagccggttt acccggagga tgtcccgggc ctccaaaggg cgcaaggggt cgcccatggt
    20101 gggtcttgct gatggttgac cgaaatagtg attgagacct cccagttgca ccccgagagc
    20161 ccccgccgtc gccgcctcga gccatccgga gttggggctg ggatggtggc gccggtcccg
    20221 cctcaatatc tttatggaat tgcggacatt cagccccagg ggcagggcgg cggcgggaat
    20281 aagggcggcg gtcaggcggg cggggaggta attgacgagg tcgtcaaggc gggcagcggc
    20341 ccagccgaat tcccggtagc gctcgttttt atagccgaag gtggaatcca tcgtgtttac
    20401 ggccttatag agcatggcgc ccagggggcc gccgagaaag gcgaagatca gcggtacggt
    20461 aacgccgtca gcggtgtttt cggcgacact ttccacggcg gcccgagtca ctccctgttc
    20521 atcgaggacg tctgtatccc ggcccacgat catgcctatg cggcgccgcg ccccgggcag
    20581 atccccccgg gcaagggagt catagacctc cccgctgtgc cgggtcaggt cccgggcggc
    20641 aagggtcgtg tagatgatca ggatagagag ggtgtcgccc agccagggat agagccggcg
    20701 ccccagcagg agcaggcccc agcagagtcc ggcggtgacg gttaggaccg cgagtaccgt
    20761 cacgatcccc gccaggcgcg gggaggagaa cagctttcgg gtgaccgttt cgctcagcag
    20821 ggcgaaacgg ccaatgaatt tcaccggatg gggaagccgg cggggatcgc cccagagcag
    20881 atccaggagc acggcgacaa cgatttgata tccgagcggc ataaccgatg atctcgtaac
    20941 tgatttattt tattaccacc aatgtagtgc gcagctttag ctgtgctata gcggacctta
    21001 atgtccgcgc tgccttgatc aactccgttc tcgcctctat attgtcattc ccgcgaaagc
    21061 gggaatccag cattatcaag aagttcttgg attccgggtc aagccaggaa tgacagaaaa
    21121 agtcgttttt aaaaggtttc actgctttgt gtcggcccgc cgtgatgagt atcatctttc
    21181 gttgtgcccg taacgggcat gagggttaga caaggcaggc cgccagtccg gtcagggcca
    21241 ccgccgtttc cgcgatttcc gcggcgg
//
LOCUS       DAOSMF010000025        19828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_3359_length_19828_cov_23.348354, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000025 DAOSMF010000000
VERSION     DAOSMF010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 19828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19828
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3359_length_19828_cov_23.348354"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(272..1441)
                     /locus_tag="PLT63_04540"
     CDS             complement(272..1441)
                     /locus_tag="PLT63_04540"
                     /inference="COORDINATES: protein motif:HMM:NF002806.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HOD98027.1"
                     /translation="MEAKLDVHKTETIYLDHSATTPVDPEVLGAMMRYFANHFGNPGS
                     IHQFGRLARAAIDNAREQTAALIGAAPGEIVFTGGGTEADNLALLGAAAAAKPGKNHL
                     ITSAVEHYAVLNTCKYLESKGFSVNYLPVDGTGTVDPDKVRRAITDRTLLISIMHANN
                     EIGTVAPLAEIAGAARQRGILIHTDAVQSAGKLPVGVDDLAVDLLSLSGHKFYGPKGT
                     GALYVRRGTPLAPIMFGGHQESGLRTGTENVPGIVGLGKACELAARELPWQMDRLLEL
                     RNRLEKLIVEKIDAVRINGHPFRRLPHVLSVSFRGVSGDAVVRELDRQGIAASAGAAC
                     TSGTLQISHVLAALRIPEDYAPGTVRFSLGKGNTEEQLAGTANILAEIIRRLRNK"
     gene            complement(1500..2033)
                     /locus_tag="PLT63_04545"
     CDS             complement(1500..2033)
                     /locus_tag="PLT63_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HOD98028.1"
                     /translation="MLKREETVLAVIDIQGNLYLAMDDREFLLANTIKLIQGIRILEI
                     PIILTEQVKIGPTIADLSFLMPDVEPLIKNSFSCCGDPRFMTALAATGKRQVLICGIE
                     AHVCAYQTSMDLMAAGYEVHLAADAVSSRTARNREIGIRKLLSAGAILTSTEMALFEL
                     LKTATDPRARDIFRIIK"
     gene            2285..2551
                     /gene="rpsT"
                     /locus_tag="PLT63_04550"
     CDS             2285..2551
                     /gene="rpsT"
                     /locus_tag="PLT63_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOD98029.1"
                     /translation="MANHKSAIKRAKQNDERRLRNTSIHSSIKTEIKTILQAVEKKES
                     DNAKAALAGAIPKISKAAAKGVFHKKNAARKISRLTLKVNALTS"
     gene            complement(2558..3142)
                     /locus_tag="PLT63_04555"
     CDS             complement(2558..3142)
                     /locus_tag="PLT63_04555"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HOD98030.1"
                     /translation="MQVCEKLKRHLIDSKAISRWFLRSLPAWLPILMATLLILTGCGY
                     HFSPGGEHIDPGIKKVFIEPFGNRTSQANLEDTFRLAVADQFIKGKRFVIVDGGNEAD
                     ALIRGSIKALSTTPLSYRTTNLAAEERMTLTVEMAFEERRSGNVIWQDDNFTGTQDYP
                     VASLGDTEASRKNALSKLANDTAERAYRMLMSGF"
     gene            complement(3282..5867)
                     /gene="leuS"
                     /locus_tag="PLT63_04560"
     CDS             complement(3282..5867)
                     /gene="leuS"
                     /locus_tag="PLT63_04560"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOD98031.1"
                     /translation="MNVKYNPQQIEEKWQHYWEEQKTFSAKEDPRKKKYYLLEMFPYP
                     SGKIHIGHVRNYTIGDVVARYKRMQGLNVLHPMGWDAFGMPAENAAIAHGIHPSVWTN
                     ENIGHMKKQLKRMGFSYDWDREVSTCEPDYYRWEQLFFLWMYEKGLAYKKTSMVNWCP
                     DCRTVLANEQVEAGLCWRCASEVTPRELDQWFFRITAYVEELLAYCDRLPGWPERVLT
                     MQKNWIGKSHGCELSFPMADGNGDIKVFTTRQDTIFGATFMLIAAEHPLVLELVKGKE
                     REGKVRQWVDKIKKHDKVLRTSDYYEKEGIFLDAYCLNPVTKQRMPIYAANFVLADYG
                     TGCVMAVPTHDQRDFEFARKYDLPLIVVIQPPNKALNVQTMTEAYVEEGVLVNSGPFD
                     GMENMKALDKIAEYLESIGRGKKTIQYRLRDWGISRQRYWGAPIPIVYCEKCGAQPVS
                     EADLPVILPRDVELTGGGGSPLARHEAFVTAACPHCGGLARRETDTMDTFVESSWYFD
                     KFCCATYNEKPGLDRKALDYWMPVDQYIGGIEHAILHLLYSRFYTKVLRDFGVLGADE
                     PFTNLLTQGMVCKETMKCRTHGFLYPDEVKEGRCVHCREEIIVGKTEKMSKSLKNVVD
                     PDYLIERYGADTARLFCLFASPPEKDLEWSDQGVEGSFRFLNRTWRIVMDYLEDIREI
                     KPFAGEVILDGDLKNLRRKTHQTIRKVSRDIEDRFHFNTAISAVMELVNALYALPRPA
                     KDDRTALAVVRETVDSVLLLLGPTVPHFAEELWESMGNGTRITDSSWPIFDPTVAAEE
                     EIVIVIQINGKVRSRISVPIDEEEASIKAQAHADEKIAGMLAGKKIVKEIYVPKKLLN
                     IVVQG"
     gene            complement(6333..7511)
                     /locus_tag="PLT63_04565"
     CDS             complement(6333..7511)
                     /locus_tag="PLT63_04565"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF014792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOD98032.1"
                     /translation="MQISSESIKIGHPDVVADIIAANVIAEIMDEEKKLGMDLSNMPH
                     CGIEVFLGKGICLVGGEVRTRVFVDIDKITRDSVLGIGYNHAAVGLNGHLMGVLNAII
                     PQSPDINIGTSMELNKNKEIGAGDQGIMYGFACDETPELLPLPYVLVSRLMRAFEDCQ
                     DPIFAPDGKGQVSVDYDDKTGKPKRLAKVLMSNAVDYRHVKGKRSGVKDEAKKLAFAC
                     LSDWVDKKTEFLFNPTGEWNALNSCSAADSGVTGRKLVVQFYGGYPGAQLGGGSVVNK
                     SPEKVDCSAAFGARYVAKNLVAAGLATKCSVQLAYAIGIAKPFSIYVNSFGTGAVPDS
                     KLETIIADNFDLTPGGMITRFGLLSGSVYRRIPKTFFMDGYKWEKTDMVKQLKQAAAK
                     "
     gene            complement(7670..8122)
                     /gene="nusB"
                     /locus_tag="PLT63_04570"
     CDS             complement(7670..8122)
                     /gene="nusB"
                     /locus_tag="PLT63_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOD98033.1"
                     /translation="MQQRRRAREYALQVLYTLDLQEREVNEALDLFWSILNNPEYLGN
                     HPPRDEGAKKFAVQLVRGTWENHAQIDDIIRIYSEHWSLGRMSRVDRSILRMATYELL
                     FCEGIPPKVSLNEAIDLGKIYGSENSGAFINGILDALYTDRLVKKPLT"
     gene            complement(8131..8595)
                     /gene="ribE"
                     /locus_tag="PLT63_04575"
     CDS             complement(8131..8595)
                     /gene="ribE"
                     /locus_tag="PLT63_04575"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOD98034.1"
                     /translation="MPKIIEGKIVAKGMKFAVVASRFNDFISSRLTDGAVDGLTRAGA
                     DEKDIQLFKVPGAFELPLAAKKLAQSGRYDAVICLGAVIRGATPHFEYVSAEVSKGIA
                     NVGLDTGVPIAFGVLTTDTIEQAIERAGTKAGNKGWDAAMTAIEMVDLFKKI"
     gene            complement(8618..9814)
                     /locus_tag="PLT63_04580"
     CDS             complement(8618..9814)
                     /locus_tag="PLT63_04580"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HOD98035.1"
                     /translation="MGVSSIKEAIEDIRNGKMIILVDDEDRENEGDLCMAAEFVTPEA
                     INFMAMHGRGLICLTLTEEHADRLRLMPMARDNRSRFGTAFTVSIEAKKGVTTGISAY
                     DRATTIRTAVAEGAKAEDLVSPGHVFPIRAKKGGVLVRTGQTEGSVDLARLAGLKPTG
                     VICEVMKDDGTMARMPDLEVFAAQHDLKIVTIADLIDFRMAHESLIRRVASADIPTHY
                     GGMFKVTVYENDVDDLQHVALVKGEIRPDDEVLVRVHSECATGDIFGSARCDCGDQLH
                     RAMQMVDQEGKGVIVYMRQEGRGIGFVNKIRAYALQDEGKDTVEANIELGFKADLRDY
                     GIGAQILVDQGVRKMRLMTNNPKKIVGLEGYGITITGRVPIVIEPNENNIRYLTTKKT
                     KMGHLI"
     gene            complement(9818..10465)
                     /locus_tag="PLT63_04585"
     CDS             complement(9818..10465)
                     /locus_tag="PLT63_04585"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HOD98036.1"
                     /translation="MFTGIIQALGTMSRLVRQGDEGLLSITTDLPLEDVLIGDSIAVN
                     GVCLTVTAKGSQTFTADVSAETLARTNLGNLHPGVSVNLEKSLRLQDFLGGHLVLGHV
                     DGLGRISEKTVRTKSLLFVFEVPDGLSKYIVEKGSVAVDGISLTVNRCVRNQFHVNMI
                     PHTAHKTTLGSKRVSDLVNIETDIIGKYVERLMQHRGGAGPGVDWKLLSEKGFLR"
     gene            complement(10473..10937)
                     /gene="nrdR"
                     /locus_tag="PLT63_04590"
     CDS             complement(10473..10937)
                     /gene="nrdR"
                     /locus_tag="PLT63_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HOD98037.1"
                     /translation="MKCPFCHHAENRVIDSRISKDGNAIRRRRECLGCGKRFTTYEVV
                     EDVLPMVVKKDGRREPFDRLKIRNGLIKALEKRPVSIDEIDAVVDKVERACQENLGKE
                     IPTAIIGEKVMEELHRLDTVAYVRFASVYRQFRDVNDFLAELKDLLNVKKEG"
     gene            complement(10934..11395)
                     /locus_tag="PLT63_04595"
     CDS             complement(10934..11395)
                     /locus_tag="PLT63_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HOD98038.1"
                     /translation="MKGENAGEQRPSWDEYFMDIAGLVARRSTCLRRQVGAVLVGDRR
                     VLATGYNGAPKGLRHCLELGCLREQQRIPSGERHELCRGLHAEQNAIIQAALYGVSVK
                     DAVLYCTNHPCVICAKMIINAGISAVIIRDPYRDALAEEILQEAGIGVRLI"
     gene            complement(11461..12726)
                     /gene="glyA"
                     /locus_tag="PLT63_04600"
     CDS             complement(11461..12726)
                     /gene="glyA"
                     /locus_tag="PLT63_04600"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOD98039.1"
                     /translation="MTSLLKTDPEVARAIWDETRRQAGKLEMIASENFVSEAVLAAQG
                     SIMTNKYAEGYPAKRYYGGCEYVDVVESLAIERCKRLFGADHVNVQPHSGTQANMGVY
                     FAVLQPGDTILGMNLSHGGHLSHGSPANFSGRLYNVVAYGVSRDTETIDFNEVEDQAK
                     KHRPKLIVVGASAYPRTIDFQMFREIADRTGAAVMADIAHIAGLVATGLHPSPVPVCE
                     FVTSTTHKTLRGPRGGLIMCKQDRAAIINSRVFPGMQGGPLMHIIAAKAVAFQEALSV
                     EFKTYQAQIIKNAQALAAALAAEGFRLVSGGTDNHLMLVDLTGQGITGKEAQEVLDQA
                     GITVNKNGIPFDTLGPQITSGIRLGTPALTTRGMKEEEMRIVAGFIARILKDTKNQAK
                     VADVRGNVAELCQRFPLYLERLKNSGFED"
     gene            complement(13029..13508)
                     /gene="rpiB"
                     /locus_tag="PLT63_04605"
     CDS             complement(13029..13508)
                     /gene="rpiB"
                     /locus_tag="PLT63_04605"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOD98040.1"
                     /translation="MIKEIAIGADHAGFPLKEAVKAFLTDQGWRVADKGTDSEQAADY
                     ADYAAAVAREIVGGGCERGILICGSGAGMVIAANKFPHIRATLCLNEEMARLCRLHND
                     SNILVLAGRMTDAATAGKIVAMWLQTQFEGGRHQRRIDKIRAIEASLTESCCRNGKR"
     gene            complement(13516..14778)
                     /gene="fabF"
                     /locus_tag="PLT63_04610"
     CDS             complement(13516..14778)
                     /gene="fabF"
                     /locus_tag="PLT63_04610"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOD98041.1"
                     /translation="MQRRVLITGLGAVSPVGNSVREAWKSVCEGRSGVGPITRFDCST
                     FDTKIAGELKGFDPLAHVNKKELRRYDDFIVYALAAADMAMEDAALGTRGAAPDERAG
                     VIIGSAIGGLATIEKEKENVLRAGPRRISPFSIPAVLANLAAGHVSIRYGLKGPINCP
                     VTACASGTYAIGDAFRMIAWNHADVMVAGGAEAAVCSLAIGGFNAMRALSTRNDQPEK
                     ASRPFDLERDGFILSEGCGLLILEEYHHALKRGARIYAEVAGYGVTSDAFHMAAPPPG
                     HEGAVRCMTVALDDAAMKPSDIDYINTHGTSTPLNDIYEAEAINTIFGTHTERLLVSS
                     TKSMTGHMLGAAGGMEAIFAVKAIVEGIVPPTINLDNPDPAAGALDFVPHAARKADIR
                     TAMSNTFGFGGANAVLIFKKFVNINQHR"
     gene            complement(14806..15042)
                     /gene="acpP"
                     /locus_tag="PLT63_04615"
     CDS             complement(14806..15042)
                     /gene="acpP"
                     /locus_tag="PLT63_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOD98042.1"
                     /translation="MTLEEKVIDIIMEQLEVTKEECVPEASFIDDLGADSLDIVELIM
                     EMEENFGIQIADEELEKIRTIQDVIDFLKAKGVK"
     gene            complement(15241..15426)
                     /gene="rpmF"
                     /locus_tag="PLT63_04620"
     CDS             complement(15241..15426)
                     /gene="rpmF"
                     /locus_tag="PLT63_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOD98043.1"
                     /translation="MPNPVKRHSRTRRNKRRSHDFLSAPAISLCPQCGEKKMPHRACP
                     NCGTYKGREVIPLEKTA"
     gene            complement(15457..15984)
                     /locus_tag="PLT63_04625"
     CDS             complement(15457..15984)
                     /locus_tag="PLT63_04625"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HOD98044.1"
                     /translation="MKINIHTIPDEGLELQEIKDRRWLPTVLAGEADVDFSLEEVKIS
                     CKIRKVDKTFFLHGTVETTIHIPCSRCLETATLPVNVPFRYTLVPAATESREEIELTE
                     EDLEYSFYEGETVDCDPLIYEQIVLQIPMKALCREDCRGLCPRCGANLNLSSCSCPDR
                     DVDERLAVLKKLKQK"
     gene            16329..17747
                     /gene="gltX"
                     /locus_tag="PLT63_04630"
     CDS             16329..17747
                     /gene="gltX"
                     /locus_tag="PLT63_04630"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOD98045.1"
                     /translation="MDKKKKIRTRFAPSPTGYLHIGGARTALFNWLYARHHGGRFILR
                     IEDTDQLRSTEESTRAILDAMTWLGLNWDEGPFYQAQRVDIHREMVERLIDEKKAYYC
                     TCTPEELEEKRKEALRTGRKPKYDGTCRDRGLAKSPGSVVRFRCPDYGTTMVRDLVKG
                     TINFNNDELDDLVIERADGYPTYNFAVVVDDAQMGITHVIRGDDHVNNTPRQILLYQA
                     LGFEVPLFGHVPMILGADKARLSKRHGATSVMAYQEMGFLPEALVNYLVRLGWSHGDQ
                     EIFSMTELIDLFDLDAVGKSAAIFNPDKLLWLNAHYIKQYPPDKLMAAVKPFWAARGC
                     DVANEDFLGRVVGDLRRRAKTLVEMTEAGQFYFAEALTYDPEAKAKFLTADVAGHLHD
                     LARDLALLADYSKEGIEGFLRRFTEARNVKLKIIAQPLRVALTGKTVSPGIDEVMVTL
                     GKERVIKRLEDALAIIAAEAGA"
     gene            17944..18018
                     /locus_tag="PLT63_04635"
     tRNA            17944..18018
                     /locus_tag="PLT63_04635"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17977..17979,aa:Glu,seq:ctc)
     gene            complement(18118..18522)
                     /locus_tag="PLT63_04640"
     CDS             complement(18118..18522)
                     /locus_tag="PLT63_04640"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOD98046.1"
                     /translation="MTSDLRQALLQWCEARLKQPADDKEHVFVCLEETYFCREYYGKP
                     FLHRQHLMENLCRRAKVKPFGFHGIRHLTASILYKKGYPLAVIQAILRHANPNTTARY
                     IRSLGLEETRGALEEGLKGPAQVIEFQKVKAV"
     gene            complement(18656..19540)
                     /locus_tag="PLT63_04645"
     CDS             complement(18656..19540)
                     /locus_tag="PLT63_04645"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOD98047.1"
                     /translation="MLLRILPLIDREATFAIKGGTAINFFARDLPRISVDIDLVYLPV
                     GERDESLRQISAALVRLSQDIENRIPGTKVTPRKAGGSEFLSGLFVRRQDTIVKIEPN
                     LVIRGSIYPPARRLISPKAIDLFKVSVESQILSDDELYAGKICAALDRQHPRDLFDIM
                     MLLRYGNFSVTMRRAFIVYLISHDRPMIELLDPGLVDIRPVFETEFQGMTLEEVGCED
                     LEKTREQLVAMIADGLTVAEKQFIVSVKEGTPQWELIGVEGIENLPAVRWKLLNIGRM
                     NQSKHKKAVRKLRDYLGV"
     gene            complement(19570..>19828)
                     /locus_tag="PLT63_04650"
     CDS             complement(19570..>19828)
                     /locus_tag="PLT63_04650"
                     /inference="COORDINATES: protein motif:HMM:NF022898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HOD98048.1"
                     /translation="EAQLIMENLVALRTDVVQRLLAACRSIKVKRLFLYMAEKKEHTW
                     LLKLDLSKVDLGKGKRMIVPHGRFDAKYQITVPATREGTPL"
BASE COUNT         4006 a         5660 c         5578 g         4584 t
ORIGIN      
        1 gcggatgtcc gggcaatgaa ccaaaccctg tcccggttca agaccggcga cgaagatggc
       61 acagacgctt cccgagtctg aacggtccac cgccggatgg ctcggctctc cccttgaggg
      121 gtagggagct acagggagct acagggaacc acagcaaaaa atattgccct ccctgtccct
      181 cccgctgaag gggagggaac tgcatattgt ccaccccgat cctccccttg gagggagcgg
      241 cgggtcaata tcccggggcg tcattctttt tttacttgtt ccggagtcgc cgaatgattt
      301 ccgccaagat gttggcggtg ccggccaact gttcttctgt gttcccctta cccagactga
      361 accgcaccgt acccggcgca tagtcttccg ggattctcaa tgccgccagg acatgagaga
      421 tttgtagggt cccggaggtg caggcggcgc cggcggaggc ggcgattccc tgccggtcca
      481 gctcccggac gacggcatcg ccggagacgc cccggaagga cacgctgagg acatggggga
      541 gcctccggaa aggatggccg ttgatccgga cggcatcaat cttttcgacg atcagcttct
      601 ccagtcgatt acgcaattcc agcagccggt ccatctgcca cggcagctcc cgggcggcaa
      661 gctcacaggc cttgcccagg cccacgatgc cggggacgtt ttccgttccg gtccgcagtc
      721 cgctttcctg atggccgccg aacatgatcg gggcgagggg ggtgccgcgg cgcacgtaga
      781 gggcccctgt ccctttggga ccgtagaact tgtgtccgga gagggagagg agatccaccg
      841 ccagatcatc cacgcccacg gggagcttgc ccgcggactg cacggcgtcg gtgtggatca
      901 ggattccccg ctgtcgggcc gcgccggcga tttccgccag gggcgccacc gtcccgatct
      961 cattgttggc gtgcatgatg ctgatcagca gggtcctgtc ggtgatggcc cggcggactt
     1021 tgtcgggatc caccgttccc gttccgtcca cgggcagata atttacggaa aagcctttgc
     1081 tctccaagta tttgcaggtg ttaagaacgg catagtgctc gacggcggag gttataagat
     1141 ggtttttccc cggctttgca gccgcggcgg cccccaggag ggccaggttg tccgcctctg
     1201 tcccgccgcc ggtgaagacg atctctccgg gggcggcgcc gatcagggcg gcggtctgct
     1261 cccgggcatt gtcaatggct gccctggcca gacggccgaa ctggtggatg cttccggggt
     1321 taccgaaatg attggcgaag tagcgcatca tcgcccccag gacttccgga tccacggggg
     1381 tggtggcact gtgatccaga tagatggttt ccgttttatg aacatcaagc ttcgcttcca
     1441 taaccgcctt tccgatcctt ttgcaaatat aaagtaaata tcaagcttca ctatccccgt
     1501 tatttgatga tgcggaagat gtcccgggcc cggggatcgg tggctgtctt caacaattcg
     1561 aaaagggcca tctcggtgct ggtgaggatg gctcccgcgg acagcagctt ccgtatcccg
     1621 atctccctat tccgggccgt ccgggaggaa acggcgtcgg ccgccaggtg gacctcgtag
     1681 cccgcggcca tgaggtccat ggatgtctga taggcgcaga cgtgcgcctc gatgccacag
     1741 ataaggacct gccttttccc tgtggcggcc agggcggtca tgaaccgggg atcgccgcag
     1801 caactgaaac tattcttgat cagcggttcc acgtccggca tcaggaagga aaggtcggct
     1861 atggtcggcc cgatcttgac ctgctcggtg aggatgatgg ggatctccag gatcctgatc
     1921 ccctggatca gcttgattgt gttggccagg aggaactccc ggtcatccat ggccagatag
     1981 aggtttccct ggatgtcgat taccgccaat accgtctctt cccgcttcag cataaaattc
     2041 tccttgttga ctgtatatgc aatcacatct gtatgaattc attcactaat aaattgtctc
     2101 cgcttcaagg ggcggattaa ggcggggatg ggttttacct tttatgaaca ttaagcttcg
     2161 ctttatgaac attaagcttc gctttcatga aacctctgga aaagagcaat taattcttga
     2221 cagccggcgg caatttatat aaaggattcc ctccaattga ataagtaaca aggaggaatt
     2281 ctttttggcc aaccacaagt ccgcgatcaa gagagcgaag cagaacgacg aacgccgtct
     2341 acgcaataca tccatacatt caagtatcaa aacggaaata aaaaccattc tccaagccgt
     2401 cgagaaaaag gaaagcgata atgccaaagc cgccttggcc ggtgcgattc ccaagataag
     2461 caaagcggcc gccaaaggtg tttttcacaa gaagaacgcg gcccggaaga tttcccgcct
     2521 taccctgaag gtaaacgccc ttacgtcctg atcccgatca aaaacccgac atcagcatcc
     2581 ggtacgccct ttcggctgtg tcattggcca gtttggaaag ggcgtttttg cgactggctt
     2641 ccgtatcgcc cagactggca acggggtagt cctgggtgcc ggtaaaattg tcgtcctgcc
     2701 agatgacatt cccgctgcga cgttcttcga aggccatctc gacggtgagg gtcatgcgct
     2761 cctcagcggc caggttcgtg gtccggtagg agaggggggt ggtcgaaagg gccttgatgc
     2821 tgccccggat caaggcatcc gcctcgttcc cgccgtccac gatgacgaag cgcttgccct
     2881 tgatgaattg atcggcgacg gccaggcgga aggtgtcttc caggttcgcc tgactggtcc
     2941 ggttcccgaa gggttcgatg aacaccttct tgatgccggg atcgatatgt tccccgccgg
     3001 gggaaaaatg gtaaccgcag cccgtcagaa tcagcagtgt cgccatcagg atgggaagcc
     3061 acgccgggag agaacggaga aaccatcgag aaatggcctt gctgtctatt aggtgccttt
     3121 ttagcttctc acacacctgc atatcgttgt acctgcttat ggaatcttaa tcccccctcg
     3181 cgaagggcaa aaacatttgt ttcgtcgttt acagaccgtc tatctcgagt agtgcaaaat
     3241 gccccgagag aaaatatgag aagcgtaccc taattttacg attaaccctg gacgacgata
     3301 ttgagtagtt tcttcgggac atagatctcc ttgacgatct tcttgcccgc cagcatcccg
     3361 gcgatcttct cgtcggcatg ggcctgggcc ttgatggacg cctcctcctc gtcaatgggg
     3421 acggagatcc tgcttcgcac cttgccgttg atctggatga caatgacgat ttcttcctcg
     3481 gcagccacgg tcggatcgaa gatcggccag gaagaatcgg ttatccgggt tccgttgccc
     3541 atcgactccc atagttcttc ggcaaaatgg gggacggtag gtcccaaaag gaggagcacg
     3601 gagtccacgg tctcccggac caccgccagg gcggtgcggt catccttcgc cggccggggc
     3661 agggcataaa gggcgttcac cagttccatg acggcgctga tggcggtgtt gaaatggaaa
     3721 cgatcctcga tgtcccggga gaccttccgg atggtctgat gggtcttgcg ccgcaggttc
     3781 ttcaagtctc catcgagaat gacctcgccg gcgaagggct tgatttccct tatgtcttcc
     3841 aggtagtcca tgacgatgcg ccaggtgcgg ttcaggaaac ggaacgatcc ctccaccccc
     3901 tggtcgctcc actccagatc tttttccggt ggggaggcga acagacagaa gagccgggcg
     3961 gtgtcggcgc cgtagcgttc gatcagatag tcgggatcca ccacattctt cagggatttg
     4021 gacatcttct ccgtcttgcc gacgatgatc tcctcccggc agtggacgca ccgtccttcc
     4081 tttacctcgt cgggatagag aaagccgtgg gtccggcatt tcatcgtttc cttgcaaacc
     4141 atgccctggg tgaggagatt ggtgaagggc tcgtcggcgc cgaggacccc gaaatcccgg
     4201 aggaccttgg tgtaaaatcg cgagtagagg agatggagaa tggcatgttc gatgccgccg
     4261 atgtactgat cgacgggcat ccagtagtca agggccttcc ggtccaatcc cggcttctcg
     4321 ttgtaggtgg cacagcagaa cttgtcaaaa taccatgaag attcaacgaa ggtatccatg
     4381 gtgtccgttt cccggcgggc tagccccccg cagtggggac aggcggccgt cacgaaggcc
     4441 tcgtgtctgg ccagaggcga tccgcccccg ccggtgagtt ccacatccct gggcagaatc
     4501 acggggagat ctgcttctga aaccggttgg gcgccgcatt tctcacagta aacgatgggg
     4561 atgggagccc cccagtagcg ctgccgggag atgccccagt cgcggaggcg gtactggatc
     4621 gtctttttgc cccggccgat ggattccaga tactcggcga tcttgtccag ggccttcatg
     4681 ttttccatgc cgtcgaaagg accggaattg accaggacgc cttcctccac atacgcctcc
     4741 gtcatggtct ggacattgag ggccttgttg ggcggctgaa tgaccacgat gagggggaga
     4801 tcatattttc tggcgaattc gaagtcccgc tggtcgtgag tcggcaccgc catgacgcaa
     4861 cccgtgccgt aatcggcgag gacgaaattg gcggcgtaga tcggcatcct ctgtttcgtt
     4921 actgggttca ggcagtaggc gtcgaggaag ataccctcct tctcgtagta gtccgaggtg
     4981 cgcaggacct tgtcatgctt cttgatcttg tccacccact gccgaacctt cccctcccgc
     5041 tccttgcctt tgaccagttc cagcaccagg gggtgctcgg cggcaatgag catgaaggtg
     5101 gccccgaaga tggtgtcctg gcgggtggta aataccttga tgtcgccgtt gccgtccgcc
     5161 atgggaaagg aaagttcgca gccgtggctc ttgccgatcc agttcttctg catggtgagg
     5221 acccgctccg gccagccggg gagacggtca cagtatgcca gtaactcctc cacataggcg
     5281 gtgatccgga agaaccactg gtccagttcc cggggcgtca cctcgctggc gcaacgccag
     5341 cagagccccg cctccacctg ttcgttggcc aggacggtcc ggcagtccgg gcaccagttg
     5401 accatggagg tcttcttata ggcgaggccc ttttcataca tccacagaaa gaagagctgt
     5461 tcccagcgat agtagtcagg ctcgcaggta gaaacctccc ggtcccagtc atagctgaag
     5521 cccatgcgct tgagctgttt tttcatgtgg ccgatatttt cattggtcca gacggaggga
     5581 tgaatcccat gggcgatggc ggcgttttcc gccggcatcc cgaaggcgtc ccaacccatg
     5641 ggatggagga cgttgagacc ctgcatccgc ttgtagcggg caacgacgtc cccgatggta
     5701 tagttgcgga catgaccgat atgaatcttc ccggaagggt aggggaacat ttccaggaga
     5761 taatactttt tcttgcgagg atcttccttt gccgaaaagg tcttttgctc ttcccagtaa
     5821 tgctgccatt tctcttcaat ctgctggggg ttgtatttga cgttcataga tcgctcccgg
     5881 gattgttttg gacgccggag aaaaacccga cggttttgcc gttcggcgtc agttatttcc
     5941 tttcgtaaca cagggaagga atgatttcaa taataaaggc gtcaccccgc cttggcggcg
     6001 ggccgaagcc aatcgtctcg aaggagctta atgaaagggg ctcagaaatt ttttccgggc
     6061 aagagaattc ctttaaaaga agctcggcgc ggtttaacca accacgccga tcctacatca
     6121 gtttttccat tccggccttg acggcttcgt aaaaaaccct catgccccgt aaagcaccac
     6181 gaagcatgat gatggcactt tcatagtaag tccggaggcg acgaaggcgc tgcctccgga
     6241 cttactatgg gtagcgttaa gcttaagcga acataaagcg aagctcaatg ttcatgatcg
     6301 cgacttttac ggattcgtcg gctctccgcc cgttattttg ccgccgcttg ctttaattgc
     6361 ttgaccatgt ccgtcttctc ccatttgtag ccgtccatga agaaggtctt tgggatgcgc
     6421 cggtaaacgc tgccgctgag gaggccgaac ctggtgatca tcccgcccgg ggtcagatcg
     6481 aagttgtcgg cgatgattgt ttccagtttg ctgtcgggaa cggcgccggt gccgaagctg
     6541 ttcacataga tggagaacgg tttggcgatt ccgatggcgt aggccaactg cacggagcat
     6601 ttggtcgcca gaccagcagc gacgaggttt ttggccacgt aacgggcgcc gaaagcggcg
     6661 gagcaatcca ccttttccgg ggacttgttg accaccgaac cgccgcccaa ctgggcgccg
     6721 ggatagccgc cgtagaactg gacgacaagt ttacggcccg tcacgccgga gtcggcggcg
     6781 ctgcaagaat ttagggcgtt ccattccccg gtggggttga agaggaactc cgtcttttta
     6841 tccacccagt cggacagaca ggcgaaggcg agctttttcg cctcgtcctt gacgccgctc
     6901 cttttcccct tgacgtgacg gtaatcgacg gcgttggaca tgaggacctt ggccaggcgt
     6961 ttgggtttcc ccgtcttgtc gtcatagtcg acagatacct gccccttgcc gtccggggcg
     7021 aatattgggt cctgacagtc ttcgaaggcc cgcattagcc tgctcacgag gacgtagggc
     7081 agggggagaa gttccggcgt ttcatcgcag gcgaaaccgt acataatccc ctgatccccg
     7141 gcgccgattt ccttgttctt gttgagctcc atgctcgtgc cgatattgat gtccggagat
     7201 tgggggataa tggcgttcaa gacgcccatg agatgaccgt tgagcccaac ggccgcgtga
     7261 ttgtaaccga tgcccaggac ggaatcccgg gtgatcttgt cgatatccac aaaaaccctg
     7321 gttctcactt cgccgcccac caggcagatg ccttttccca gaaacacctc gatgccacag
     7381 tgaggcatat tgctgaggtc catgccgagc ttcttttcct catccatgat ctcggcgatg
     7441 acgttagcgg cgatgatgtc cgccaccacg tcgggatgtc caatcttgat gctttccgaa
     7501 gaaatctgca taattttatc tccttttgtt gaattgattt ccggccaaaa aaaaacccgc
     7561 gctcaagcac gggttcaatc cagacacctg cggtgcttta gcacatttat agagcggagc
     7621 aatttacctt taaatcaccg ctttggcgcc cttctaccat atggatacat tatgtcaagg
     7681 gtttttttac cagccggtcc gtgtagaggg cgtcgaggat gccgttgata aaggccccgg
     7741 aattctccga gccgtatatc tttcccagat cgatggcctc gttcagtgag accttcgggg
     7801 ggattccttc acagaacaac agctcgtaag tcgccatcct gaggatgctc cgatccaccc
     7861 gggacatccg gccgagcgac cagtgctccg agtagatgcg gatgatgtcg tctatttgag
     7921 cgtggttttc ccaggttccc cggacgagct ggacggcgaa ttttttcgcc ccctcatccc
     7981 ggggggggtg atttcccaga tattcgggat tattcagtat cgaccaaaag agatccaacg
     8041 cctcgtttac ctcccgctcc tggagatcca gggtgtaaag cacttgcaag gcatactccc
     8101 gcgcccttct tcgctgttgc atggctggtt tcagatcttt ttgaacagat ctaccatttc
     8161 gatggcggtc atggcggcgt cccagccctt gttgcccgcc ttggtgccgg cccgctcgat
     8221 ggcctgctcg atggtgtccg tcgtcaggac tccgaaggcg atggggacgc cggtatcgag
     8281 gcctacgttg gcgatgccct tggatacctc ggcgctgacg tattcgaaat ggggggtcgc
     8341 cccgcggatc acggcgccga gacagatcac ggcgtcatag cggccgctct gggcgagttt
     8401 cttcgcggcc agaggcagtt cgaaagcccc ggggaccttg aaaagctgga tgtccttttc
     8461 gtcggcgccc gcccttgtca atccgtccac cgctccgtcc gtgagcctgc tggagatgaa
     8521 atcgttgaac ctgctggcca ccacggcaaa cttcattccc ttggcgacta tctttccttc
     8581 gatgattttt ggcatcttcg ccctccgtat cggtaattta tatcaagtga cccatttttg
     8641 tctttttggt cgtcaggtag cgaatgttgt tttcattggg ttcgatgaca atcggcaccc
     8701 ttcccgtgat ggtgatgccg tagccctcca gtcccacgat ctttttgggg ttgttggtca
     8761 tgagccgcat cttccgcacg ccctggtcaa ccaggatctg ggcgccgatg ccgtaatccc
     8821 ggaggtcggc cttgaacccc agctcgatgt tggcttcgac agtgtctttg ccctcgtctt
     8881 gaagggcgta ggcacggatc ttgttgacga agccgatgcc ccggccttcc tggcgcatgt
     8941 aaacgattac gccctttccc tcctggtcca ccatctgcat ggcccggtga agctgatcgc
     9001 cgcagtcaca tcgcgccgat ccgaagatat cgccggtggc gcactcagaa tggacgcgaa
     9061 ccagcacctc gtcatccggc cggatttcgc cctttaccaa ggcgacatgc tggagatcgt
     9121 ccacatcgtt ctcataaacc gtcaccttga acatcccgcc gtaatgggtg gggatgtcgg
     9181 cgctggcgac gcgcctgatc agcgattcgt gcgccatgcg gaaatcgatg aggtcggcga
     9241 tggtcacgat cttcagatcg tgttgggcgg cgaagacctc cagatcaggc attcgggcca
     9301 tcgtgccgtc gtccttcatc acctcgcaga tgactccggt cggtttcagc cccgccaggc
     9361 gggccagatc gacggaacct tccgtctggc cggtccggac caaaactccg ccctttttgg
     9421 cccgaatagg aaagacatgc cccggactca ccagatcttc cgcctttgca ccctcggcga
     9481 cggcggtgcg aatggtcgtg gcgcggtcgt aagcggagat gcccgttgta acccccttct
     9541 tggcctcaat ggacacggtg aaggccgtcc cgaaacggga acggttgtcc cgagccatgg
     9601 gcatcagccg gaggcggtcg gcatgttcct ccgtaagggt aaggcagatc agaccccggc
     9661 cgtgcatcgc catgaaattg atggcttcgg gggtgacgaa ctcggcggcc atgcacaggt
     9721 cgccctcgtt ttctcggtcc tcgtcgtcca cgaggatgat cattttgcca ttgcggatat
     9781 cctcaatggc ttccttgatt gaactgactc ccatggttca cctcaaaaaa cctttttccg
     9841 ataataattt ccaatccacg ccggggccgg cgccgcccct gtgttgcatc agccgctcta
     9901 catacttgcc gatgatatcc gtttcgatat tcaccaagtc ggataccctt ttcgacccca
     9961 aggtagtttt atgggccgtg tgagggatca tgttgacatg gaattgattc cgtacgcaac
    10021 ggttgacggt gagactgatg ccgtccaccg ccacggaacc cttttccacg atatatttac
    10081 tcagaccgtc cggaacctcg aagacaaaaa gcaacgattt ggtccgcacc gtcttctccg
    10141 agattcttcc cagaccgtcc acatggccca gcacgagatg cccgccgaga aaatcctgga
    10201 gacgcaggga cttctccaag ttgacggaca cgccgggatg caggtttccc agattggttc
    10261 tagcgagggt ctccgccgag acatcggcgg tgaaggtctg cgatcccttg gcggttacgg
    10321 tgaggcagac gccgtttacc gcgatgctgt caccgatcag tacatcttcc aaaggcaggt
    10381 ccgttgtaat ggacagcagg ccttcatccc cctgccggac caaccgagac atcgttccca
    10441 gcgcctggat gatgccggta aacatgaata gttcaacctt ccttctttac gttcagcagg
    10501 tctttcaact cggccagaaa gtcattgacg tcccggaact gccggtaaac cgaggcgaag
    10561 cggacatagg ccacggtatc gaggcggtgg agctcctcca taaccttctc cccgatgatc
    10621 gccgtgggga tttccttccc caggttttcc tggcatgccc gctccacctt gtccactact
    10681 gcatcgatct cgtcgatgct taccggcctc ttttccaggg ccttgatgag accgttgcgg
    10741 atcttcagac ggtcgaaagg ttcccggcgg ccgtctttct tcaccaccat gggcaggacg
    10801 tcctcgacga cctcgtaagt ggtgaagcgc ttgccgcatc ccaggcattc ccgacgccgc
    10861 cggatggcgt tgccgtcctt gctgatccgg gaatcgatga cccggttttc tgcgtgatgg
    10921 cagaaggggc acttcatatc agtcttaccc cgatgccggc ctcctggagg atttcctccg
    10981 ccagggcatc ccggtatgga tcgcgaataa tcactgcgct gatcccggca ttgatgatca
    11041 tcttggcgca gatcacacag ggatgattgg tgcagtaaag tacggcgtcc ttcacgctta
    11101 ccccgtagag ggcggcctgg atgatggcgt tctgctccgc atgtagcccc cggcagagct
    11161 cgtggcgttc cccggagggg atgcgctgct gctcccggag acatcccagt tcgagacagt
    11221 ggcgaagccc cttgggggcg ccgttgtatc cggtcgccag gacccggcgg tcgcccacca
    11281 ggacggcccc gacctgccgg cggagacagg tggagcgccg ggcgacgagg ccggcgatgt
    11341 ccatgaagta ttcgtcccag gagggtctct gctccccggc gttttccccc ttcatcaaat
    11401 atgcctttaa aatcaagtcg ttagtgctga tttgtgcttg tgagatgcaa cctccgtacc
    11461 tcaatcctcg aacccggaat tttttaaacg ttccagatag aggggaaacc gctggcagag
    11521 ttccgccaca ttcccgcgga catccgccac cttggcctgg ttctttgtat ccttgagtat
    11581 ccgggcgatg aagccggcga caatccgcat ctcctcctcc ttcatgcccc gggtggtcag
    11641 cgccggggtg cccagccgga tgccgctggt gatctgggga cccagagtgt caaaagggat
    11701 gccgttcttg ttgacggtga ttcccgcctg atcgaggacc tcctgggcct ctttgcccgt
    11761 gataccctgc ccggtgagat ccacgagcat cagatggttg tccgtgccgc cggaaacgag
    11821 gcggaagccc tcggcggcca gggccgcggc cagggcctgg gcgttcttga tgatctgggc
    11881 ctgataggtc ttgaattcca ccgacagggc ttcctggaag gccaccgctt tggcggcgat
    11941 gatatgcatc agcggtccgc cctgcatccc cggaaagacc cggctgttga taatggcagc
    12001 acggtcctgc ttgcacatga tgagtcctcc ccggggaccc cggagggtct tgtgggtggt
    12061 ggaggtgaca aactcgcaaa ccggtacggg ggaaggatgc agcccggtgg cgacgaggcc
    12121 ggcgatgtgg gcgatgtcgg ccataacggc ggcgccggtg cggtcagcta tttcccgaaa
    12181 catctgaaaa tcaatggtgc gggggtaagc gctggccccc acgacgatca gtttggggcg
    12241 atgtttcttt gcctggtctt ccacctcgtt gaagtcgatg gtttcggtgt cccgggagac
    12301 gccataggcc accacgttat acaaccgccc ggaaaagttg gcggggctgc cgtgggagag
    12361 gtgaccgccg tgggacaggt tcataccgag aatcgtgtct ccaggctgaa ggacggcgaa
    12421 atagaccccc atgttggcct gagttcctga atggggctga acgttcacat ggtcggcgcc
    12481 gaagagccgc ttgcagcgct cgatggccag ggactccacg acatccacat attcgcagcc
    12541 gccgtagtag cgcttggccg ggtatccttc ggcatacttg ttggtcatga tggatccctg
    12601 cgccgcgagg accgcttcgc tcacaaagtt ttccgaggca atcatctcca gcttgccggc
    12661 ctggcgccgt gtttcatccc agatagcccg ggccacttcc ggatcagtct tgagtaatga
    12721 tgtcattaat tttccttccc tcctgtcgtt gatgtgccct tatcacccgc cgacccggcg
    12781 cggccgccgc cggaaaaggc agggcgggtg atatctcttc atcccccgct ttttgtgcgt
    12841 ttacataacc ttaaccaagg catttttcaa acggtatctt tgacggcatc gcaaccgttt
    12901 ttgccttggc gctgtgcttc ctcgttcgtc ccggaggcgt ccctacaggt acttctcatt
    12961 cctcagaatt ggcgcgcctt gccttgggtg ctttccgctg gtcttttcat atcgttattt
    13021 ttaactcctc atcttttacc gttccggcag caggattcgg tgagggacgc ttctatggcc
    13081 ctgatcttgt cgatacggcg ctggtgccgt cctccctcga actgggtctg cagccacatc
    13141 gccacgatct ttccggcggt ggcggcatcc gtcatccgtc cggccagcac gaggatgttg
    13201 ctgtcattgt gcaggcggca caggcgcgcc atctcttcat tcagacagag ggttgcccgg
    13261 atgtgcggaa acttgttggc ggcgatgacc atcccggcgc cggaaccgca gatcaagatt
    13321 ccccgttcac agccgccgcc gacgatctcc cgggccaccg ccgcggcgta atcggcatag
    13381 tccgccgcct gttcactgtc cgttcctttg tcggctaccc tccagccctg gtcagtcagg
    13441 aatgctttga ctgcttcctt gagcgggaat ccggcatgat cagcgcctat ggcaatctct
    13501 ttaatcattc caaaatcagc ggtgctgatt aatattcaca aatttcttga agatcagtac
    13561 ggcattggcc ccgccgaagc cgaaggtgtt ggacattgcc gtccggatat ccgctttcct
    13621 ggccgcgtgg ggaacgaaat ccagggcccc ggcggctgga tcgggattat cgagattgat
    13681 cgtgggggga acgattccct ccacgatagc ttttacggca aagatagcct ccatcccccc
    13741 ggcggcgcca agcatgtgcc ccgtcatgga tttggtggaa gagacgagca gacgttcggt
    13801 gtgggttccg aagatggtgt tgatggcctc ggcttcatag atgtcgttga gcggggtgga
    13861 ggtaccgtgg gtgtttatgt agtcgatgtc ggacggcttc atggccgcgt catccagggc
    13921 cacggtcatg caccgaaccg cgccttcatg tcccggaggg ggggccgcca tgtgaaaggc
    13981 gtcgctggtg acgccgtatc cggcgacctc ggcgtagatc cttgccccgc gcttcaaagc
    14041 gtggtgatac tcttccagga tcagcaagcc gcatccctcg gaaagaataa acccgtccct
    14101 ttccaggtcg aagggacggc tggctttttc cggctggtcg ttgcgggtgg aaagagcccg
    14161 catggcgttg aatccaccga tggccaggga acacaccgcg gcttccgcgc cgccggccac
    14221 catgacatcg gcgtgattcc aggcgatcat acggaaggcg tcgccgatgg cgtatgtccc
    14281 tgacgcgcag gccgtgacgg gacagttgat ggggcctttc aatccatagc gtatggagac
    14341 atgaccggcg gcaaggttgg ccagtacggc gggaatggag aaaggcgaaa ttcttcgggg
    14401 accggcgcgg agcacgttct ccttttcctt ctcgatggtg gcaaggccgc cgatagccga
    14461 tccgatgatg acgccggccc gttcatcagg ggcggcgccc cgggtgccga gggcggcgtc
    14521 ctccatggcc atgtcggcgg cggccaaggc atagacgata aaatcgtcat accgtcgcag
    14581 ttccttttta tttacatgcg ccagcggatc gaaacccttc aattccccgg cgatcttcgt
    14641 atcgaaggtg gaacaatcga atctggtgat ggggcccacg ccggaacgtc cctcgcacac
    14701 acttttccaa gcttcccgga ctgagttccc caccgggctg acggctccga ggccagtgat
    14761 caatacgcgt ctctgcaatg acctactccg ggcgtggttg cggtgttact tcacgccttt
    14821 ggctttcagg aagtcgatga cgtcctggat ggtgcggatc ttctccaact cttcatcggc
    14881 aatctgaatg ccgaaatttt cttccatttc catgattagc tcgacaatgt ccagggagtc
    14941 cgctccgaga tcatcgataa aggacgcctc gggaacgcat tcctccttgg tgacctccag
    15001 ttgttccatg atgatatcta tgaccttttc ctcaagcgtc atatcttgct cctcctgaat
    15061 gtagtgttta tactgcgtgt taatatgcaa gccgctttgt gagcggatta cctattcggt
    15121 gtcgctgtcc gttgctgccc gaccggcggg aaaagctttc cgtttccgga accatgatcc
    15181 taccccaatt gtcccgttag taacaaaacg ggatggcccg gttgattatc tgccggacgg
    15241 ttatgcggtc ttttcgaggg gaattacctc ccggcccttg taagtgccgc agttggggca
    15301 ggcgcggtgg ggcattttct tctccccgca ctgggggcac agggaaatgg ccggtgcgct
    15361 aaggaaatca tgggatctcc gtttgtttct tctcgtccgg gaatgtctct ttactgggtt
    15421 tggcatgatt ctaatcctct ttatttatat ttgatattac ttctgcttga gttttttcag
    15481 gaccgccagg cgttcgtcca catcccgatc cgggcaggag cagctgctca gattgagatt
    15541 ggcgccgcac cgggggcaaa gtccccggca gtcttcccgg cagagggcct tcatggggat
    15601 ttgcaggaca atctgctcat agatcagggg atcgcaatcc actgtctctc cttcgtagaa
    15661 gctgtactcc aggtcctcct ctgtcaattc gatttcttcc cggctctctg tagccgccgg
    15721 tacaagggta tagcggaaag ggacgttcac gggcagggtg gccgtctcca ggcagcgcga
    15781 acagggtata tggattgttg tttccacggt accatgcaaa aaaaacgttt tatccacctt
    15841 ccttatttta caagatattt ttacttcttc cagagaaaaa tcaacatcgg cttctccggc
    15901 gaggaccgta ggcaaccagc ggcggtcctt gatctcctga agttccaacc cttcgtcggg
    15961 tatggtgtga atgttgattt tcattgaaaa atgccacctt ttttattttc tgcatcggaa
    16021 agatgatcta tgctgccgat tccttccgcc tcactcacct tgaggcgccg tcacgcttta
    16081 ccgtatggcc gcccctgcgg tcaggtgtcc ttgccggtaa aaatgcaggc ttcaataaag
    16141 tggtgaagta aattataaaa tgattgcgat gtcaaggaca atttctccaa atgtaacttg
    16201 acaaatttgg acggccgaac ctataagaca ccatcgcttc gcaccgggcg ggaaatgttg
    16261 ccgctggcca cgagggagaa ggcgagaccc ccctggggac ggagccaatt tcgcaaacag
    16321 gattcagcat ggacaaaaag aaaaaaatca gaacccgctt tgccccgtcg ccgacggggt
    16381 atctgcatat cggcggcgcc aggaccgccc tgttcaactg gctctatgcc cgtcaccacg
    16441 gcggccggtt catcctgcgc attgaggata cagatcagct gcgttccacc gaggaatcca
    16501 cccgggccat ccttgacgcc atgacctggc tgggccttaa ctgggacgag ggcccgtttt
    16561 atcaggccca acgggttgat attcatcggg agatggttga gaggctcatc gacgaaaaaa
    16621 aggcctatta ttgtacctgc acacctgaag agctggagga gaagagaaaa gaggccctgc
    16681 gcaccggccg gaagccgaaa tatgacggta cctgccgtga cagggggctg gctaaatcgc
    16741 ctggctcagt tgtccgcttc cgttgccccg attacggtac aaccatggtc agggacctgg
    16801 tgaagggaac gataaacttc aacaacgatg aactcgatga cctggttatc gaaagggcgg
    16861 acggttatcc gacctacaat ttcgccgttg tggtggatga cgcccagatg ggcatcaccc
    16921 atgtcatccg gggggatgat catgtaaaca acaccccccg ccagatactc ctttaccagg
    16981 ccctgggctt tgaagtgccg ctgtttggac atgtccccat gatcctaggc gccgacaagg
    17041 cgaggctgag caagcgccac ggcgcgacgt cggttatggc ctatcaggag atgggatttc
    17101 ttcctgaggc cctggtgaac taccttgtcc ggctgggatg gtcccacggg gatcaggaga
    17161 ttttttccat gaccgaactc attgacttat ttgatctgga cgccgtggga aagtcggcgg
    17221 cgatcttcaa tcccgacaag ctactctggc tgaacgccca ctacatcaaa cagtatcctc
    17281 cggataaatt gatggccgcc gtaaaaccct tctgggcggc caggggatgt gatgtggcga
    17341 acgaggattt cctcggccgg gtggtggggg acctgcggag acgggcaaag accctggtgg
    17401 aaatgacgga agccggccag ttttattttg ccgaggcctt gacctatgat ccggaagcga
    17461 aggctaagtt cctgacggct gatgtcgccg gacatcttca cgacttagcc cgggatctgg
    17521 ccctgctcgc cgattattcg aaggagggta tcgagggatt cctgcgccgg ttcaccgagg
    17581 cgcggaacgt gaaactcaag atcatcgccc agccgctacg ggtcgccctg acgggaaaga
    17641 cggtgagccc cggcatcgat gaggtgatgg tcaccctagg caaggagcgc gtcattaaaa
    17701 gacttgagga tgccttggcc ataatcgctg cggaggccgg cgcctgacgg cgttgaggat
    17761 gggccggcag aaaatgggtt caatacccgt tgccattttc accttgactt ttggtggatt
    17821 atcgaataga tactcgctcc tgcttaaaaa gcacctgggg gagtgttgtt gctaagaaaa
    17881 caggctcgcg atcctgatgg ccgaattccg aatctttatc cccggaagac aattcacgat
    17941 cgtggtccca tcgtctagtg gttaggacgc tggcctctca cgccggaaac cggggttcaa
    18001 gtccccgtgg gactaccaat aaattcgata gtttacaaat accaactatc gccatatacc
    18061 tccggacaca agtccggaca caaaatgccc tcagagctcc tttctctggg ggttttttca
    18121 tactgccttg accttctgga attcgatcac ctgagccggt cccttcagac cttcctcaag
    18181 cgctcccctt gtttcttcca atccgaggct gcggatatag cgcgccgtcg tattcggatt
    18241 tgcatgtctc aggatcgcct gaatgactgc cagcgggtat cctttcttat acaggatcga
    18301 cgccgtcaga tgcctgatgc cgtggaaccc gaagggtttg actttagccc ggcgacagag
    18361 attctccatc agatgttgcc gatggaggaa tggttttccg taatactccc tgcaaaagta
    18421 ggtttcctcc aggcagacaa acacgtgctc tttgtcatcc gcaggctgct tcagccttgc
    18481 ctcgcaccat tgcagcagcg cctgccgcag atcggatgtc atagtgagga cataatgata
    18541 ccccagcgaa accactatgc aatcaacagg gtcaactttt tcgtaaaaag ggctcttcgg
    18601 tggccttatt tgcaaaaact attcattgaa aagtgatagt cccattaaag cgggcctaca
    18661 ctcccaggta gtctctcaat ttgcgaactg cctttttgtg ttttgactgg ttcatacgac
    18721 cgatgtttaa aagcttccac cggacggcgg gcaggttctc aatcccttca acaccgatca
    18781 attcccactg gggcgtccct tctttcacgg agacaatgaa ttgcttttcc gcgacagtca
    18841 acccatctgc gatcatggca accagttgct ctctggtctt ttccaggtct tcgcagccaa
    18901 cctcttccaa agtcatgcct tgaaattcag tttcaaaaac aggccggata tcaacaaggc
    18961 ccggatcaag aagctcaatc ataggccgat catggctgat caggtaaacg ataaacgccc
    19021 gtcgcatggt gacgctgaag ttgccatatc tcagaagcat catgatatcg aagaggtctc
    19081 gcgggtgctg gcgatccagg gcggcacaga ttttcccggc atataattca tcgtcagaca
    19141 agatttgact ctctacggaa accttaaaaa gatcaattgc tttgggagag atgagtcttc
    19201 tcgcaggcgg atatatagag ccccgtatga ccagattcgg ttcgattttg acaatcgtat
    19261 cctgccgccg cacaaacaat ccgctcagga actcggaccc tccggctttc ctgggagtaa
    19321 ccttcgttcc cggaatcctg ttttcgatgt cctgagataa cctgacaagc gcagcgctta
    19381 tctgccggag ggattcatcc ctttcaccaa cgggaagata tacgaggtca atatctacgg
    19441 agatacgggg aagatccctg gcaaagaaat tgatcgccgt cccgccttta atggcgaatg
    19501 tcgcttctct gtcaatcaat ggaagtatcc gcaggagcaa ttcggcttgt ttgaagtaaa
    19561 cgttatcgat cataaaggcg tcccttcacg agtcgcagga acggttatct gatatttcgc
    19621 atcaaaccgg ccatgtggta cgatcatccg ctttcctttt ccgagatcga ctttcgataa
    19681 atcgagcttc aataaccagg tgtgttcctt tttctccgcc atatagagga agagcctttt
    19741 cacctttatg gaccggcatg ccgccagcag cctctggaca acatcggtac gaagggcaac
    19801 caggttttcc atgatcaatt gggcctcg
//
LOCUS       DAOSMF010000026        19528 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_3412_length_19528_cov_20.968931, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000026 DAOSMF010000000
VERSION     DAOSMF010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 19528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19528
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3412_length_19528_cov_20.968931"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(234..1826)
                     /locus_tag="PLT63_04655"
     CDS             complement(234..1826)
                     /locus_tag="PLT63_04655"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOD98049.1"
                     /translation="MINYPQQGKRLLTITKIPPAAKCSGQPAGYLHLILVIVMATMLI
                     NGCTARYFRPASPPTDPCQIKELGNLPERESWHGFVFNGEKVGFLHMKIEPVAGAITY
                     RVTAEANLRIRFLGLNKKIIMKSVDIVNPDLTLVSFLYEQDVGEKKLILTGAVAAGSL
                     SVEQRSGGVTKKIETILDSPLYPSSVINLYPVLQGMKLGTGYKYSVYDPQTQSIAGVS
                     QSIVSFEESRELDLEPAFKVETGMLGHTVSSWINCRGETIFELGMSGVLITFKESEEN
                     AKSFLAEASLSKKDLIYDFSVIKTDKPITCPRTATTLEAAVAGISGLLPPLRESYQEV
                     VETLHEGKTVRIYRIRKTAPMPADLRKSALPLRDRSRYLAPSGHIESDSPEIRKAAAA
                     VVSGTRTPREKVVRLAGWVAAEVRDEAVDSYSALEVLHNRRGECKAHTMLYTAMARAA
                     GIPSRVVGGIVYMEGMGFLYHAWAESYLDGWIAVDPTFNQVGVDATHIKLADGQDWSA
                     LIQLSIVIGRIGVTIHDFSCPP"
     gene            complement(2013..2411)
                     /locus_tag="PLT63_04660"
     CDS             complement(2013..2411)
                     /locus_tag="PLT63_04660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HOD98050.1"
                     /translation="MKKQRIRILMVRLGMDAHWRGSIVVARALRDAGMEVIYGGNQMP
                     ANIVKMALQEDVDIIGLSSLSGNHLMLAPEVVKLLKKEKAQDIKVIFGGTVPPDDVPI
                     LKKAGIAAVFGPGSSLQKIIASVEALKRNY"
     gene            complement(2450..4135)
                     /locus_tag="PLT63_04665"
     CDS             complement(2450..4135)
                     /locus_tag="PLT63_04665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOD98051.1"
                     /translation="MKTGIFNQNRIKEVARETADWEENSYSCQQRLKDKYETLSGFKV
                     KPLYTPDDVAGLDYLRDLGYPGKGPFIRGVHPTMYRGRTWTHRQLAGFGPPEETNKRY
                     KFLLQQGATGINGVFDYPTLRGYSSDDPDVYADVGRGGVAIDTIGDMRTLFDDIPIDK
                     VSVSLVTCQPICNISVQSMYFANALDRGIPLANLAGTSQNDFLMETATTIAPGVLPPR
                     ASFKISCDAIEFCTKYAPQWNPISFAGYNYREAGCTAPQEIAFVIAHALACAEDMLAR
                     GLKIDNFSKRLSFFLSAHNDFFEEIAKYRAARRVYYHVMKKRYHPQDPRSLMFRFHVQ
                     TAGVALTAQQPLNNIARSAYHALAAVLGGAQSVHVDAYDEALCTPTELSSVTALRTQQ
                     ILRNETGVTNTIDPLGGSYYLESLTNSMEAEVLRILDDIDRIGGLVKAVEKGWIHGEI
                     AAAAYKYQLDVESGAMPIVGVNCYQIEDEVLPIELFSSPETVKVQTRKLKKIKKTRNA
                     GAVAKSLDAIAGCCERNKNLMKLMVGEVKNFLTEGEISRTLKRVYGVWEPPLF"
     gene            complement(4339..5724)
                     /locus_tag="PLT63_04670"
     CDS             complement(4339..5724)
                     /locus_tag="PLT63_04670"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOD98052.1"
                     /translation="MNTILIIDDQKLIRDSLKLTLSDEGYDVLSAENGKQGIAVLKKR
                     SVDLVLLDLKLPDSNGIDVLKQIKALNPECTVIMMTGYGSIENAVAAMKLGAFDYINK
                     PFKSRHISTIIKLALETQKLQTEVNELRDRNRSLFKPDRIIGQSRAMQDIQETIAKVS
                     QIGSSTVLIHGESGTGKELVAKAIHYGSPRLNGPYIEINCSAIPYSLLESELFGYEQG
                     AFTDAKRARKGLFEQSSGGTLFLDEIGDMDLIIQSKLLSVLQEKRFRRLGGQRTIQVN
                     TRIIAATNHNLKERIRQNKFREDLYYRLNVLTIFIPPLRERKEDIVPTARYFLDLLNN
                     DYRKNVKGISEDAITAMLNYSWPGNVRELRNTMERIMIIDNPEIILLKHLPREITDES
                     YADPVTGAAEINLPDSGLDIKELTSALQSKLIKQALLKTGGNKTEAAKLLRLDRFSLR
                     YLMNKHRLPDK"
     gene            complement(5737..7281)
                     /locus_tag="PLT63_04675"
     CDS             complement(5737..7281)
                     /locus_tag="PLT63_04675"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD98053.1"
                     /translation="MDRVVEAIVQTVTQDLNFNRCSVYLLNKTNHKLECKYITGFSPE
                     TENYVKERPFDIKKHDCIETRVAVTGEPVLVKNFSSSDNLTDLDRRVTTRMGRGCTLY
                     VPLKIKGDIIGILGVDKKHGEPEINEKEFESLSILANYASIIIENSKLLQDLMNEKKF
                     SENVLNSSINGILTVDITGRITSLNPAVEKMFGMMKDTLINRFIWEIFPLPEWIECTR
                     KAVFHLHNMKGMEFTLNPDDKILNVTTSHIMNEGESLIGFMFLIQDITGERKRDEYLQ
                     RVNRLISLGELAAGIAHEIRNPLTGIGVVLDFLSRKKLPRRDLELINEANGEIERLEK
                     LISDLLTFARPKKFNFELADIRNIINSIYLLINEQCNHRGIKMFIRIDDDIDGLYVDQ
                     ERIRQGLLNIVINAIKAMPSGGNLTIKAAELTGDSGSGDPERRIEITIGDTGVGIATE
                     NIGRIFDPFFTTNNEGTGLGLSITHSIIKEHSGIVKVDSELGKGTEFTIILPCDCRKA
                     VEYLDA"
     gene            complement(7686..8582)
                     /locus_tag="PLT63_04680"
     CDS             complement(7686..8582)
                     /locus_tag="PLT63_04680"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOD98054.1"
                     /translation="MEASLNQLKVFHVAAKVQSFTRAAEALFLTQPGISKHIKDLEEY
                     YGTRLFDRLGKKVVLTQAGEILYARTEIIFGIIEQMKIEIDELQGMDRGVLNVGASNT
                     LGIYILPAILGRFRSRYPRVNINLDIAMNKQVVGNLIANSTDVGFLGAPVNDERLKME
                     PFRQDELVLIVPATHEWSLWDAVDAHDLLGVTFLYPRKGSGTRDIIEKKLAATGIILR
                     NTLEIGNTEAVKKAVEAGLGVSILSKASVLREEYLGVIKSLRVSGVDLKRTFYFAYRK
                     DKYLSKITGAFLQFAVYQQPAV"
     gene            8679..8864
                     /locus_tag="PLT63_04685"
     CDS             8679..8864
                     /locus_tag="PLT63_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98055.1"
                     /translation="MDNADREKIEKIISDRKAAHAFYEDNSQTYVTFARMANEAFAAG
                     ELGRKTPEGHQLLSRKE"
     gene            8913..9281
                     /locus_tag="PLT63_04690"
     CDS             8913..9281
                     /locus_tag="PLT63_04690"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HOD98056.1"
                     /translation="MLDKTDTDKIEKVLGDLKYVHKSFTFRDSKCYGHYMQLLNHAMV
                     DRSLTRMTKELIAVGISAYANCEPCMTVHVREALRAGATDEQVEEAVDVAMEMGGGPV
                     VARGGAYIFKLLEYFRGKGR"
     gene            complement(9457..10254)
                     /gene="trpA"
                     /locus_tag="PLT63_04695"
     CDS             complement(9457..10254)
                     /gene="trpA"
                     /locus_tag="PLT63_04695"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HOD98057.1"
                     /translation="MNRIEKRFQALRSRGEKALILYLTAGDPDLQRTEALIPILDEAG
                     ADILEIGVPFSDPTADGPVIQEAAQRSLRAGTNLAAIMDMIARLRRGSDIPIVLFTYY
                     NPVFAYGNERFARQAAAAGVDGLLVVDLPPEEAAELRQFTDPRGLDFITLVAPTTGDR
                     RLAGIVKDAAGFIYYISVTGVTGTKTPEAAAVLPDLQRIRRRTDLPVAVGFGISTPAQ
                     VRGFAAQADGVVIGSALVKWLATHSEDPDLLPQAAAYVRELKAATGP"
     gene            complement(10251..11450)
                     /gene="trpB"
                     /locus_tag="PLT63_04700"
     CDS             complement(10251..11450)
                     /gene="trpB"
                     /locus_tag="PLT63_04700"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOD98058.1"
                     /translation="MKKNLPDKQGHFGIFGGRYVAETLMPALIELEEAYRRAAQDPEF
                     RREFRHYLHDYAGRETPLYFAENFTAKAGGAKIYLKREDLNHTGAHKINNTLGQALLA
                     RRMGKKRVIAETGAGQHGVATATVAALFGMECEVFMGEEDIRRQAPNVFRMEMLGARV
                     TPVASGTATLKDAMNEAMRHWVARVRDTFYVIGTTAGPHPYPRMVRDFQSVIGREARR
                     QILKKEGRLPDFLVACVGGGSNAMGLFYPFRQDGGTAMIGVEAGGKGIPTGLHAASIS
                     AGAVGVLHGNKTYLLQDEHGQVRDAHSISAGLDYPGVGPEHSFFHAAGRARYLSVTDA
                     EALAAFRLLTTCEGIMPAMESAHALAYTLKLAPALGRDKIIVVCLSGRGDKDMGIVAA
                     QKEVELP"
     assembly_gap    11782..11881
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11882..12465)
                     /locus_tag="PLT63_04705"
     CDS             complement(<11882..12465)
                     /locus_tag="PLT63_04705"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF002298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HOD98059.1"
                     /translation="MTEIKICGITRPEDAAHAAACGADALGFIFYRKSPRGISPAAAA
                     AIIARLPARVCKVGVFVNEEAAVIRETAATCGLDMLQLHGDESPAYCRGIADRNLLIK
                     ALPLTTAADLHRAALYPVTAILVDARDDDRYGGTGKSASWELAARLGKTGRLILAGGL
                     NGENIHAALRTVRPRAVDINSGVETAPGVKDPAKV"
     gene            complement(12462..13256)
                     /gene="trpC"
                     /locus_tag="PLT63_04710"
     CDS             complement(12462..13256)
                     /gene="trpC"
                     /locus_tag="PLT63_04710"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HOD98060.1"
                     /translation="MENNFLEKIVAAKEAELAETKRLAPLREMQAILCEVPPPRNFQE
                     ALTGRECAIIAEIKKASPSRGVIREDFDPHAIAGIYEAHGAAAISVLTERSFFQGDGR
                     YLAEIRKTVRIPLLRKDFLVDPYQIYETRCLGGDALLLIARALPGAILAELIALTREL
                     GMTPLVEVHDEEELARSLAAGAPAIGVNNRNLATFVTDLGTSLRLAPLIPAGVVKVSE
                     SGITGRRDVERLRQAGYDALLVGETLMRSEDMGRKLRELRGEEDRP"
     gene            complement(13435..14448)
                     /gene="trpD"
                     /locus_tag="PLT63_04715"
     CDS             complement(13435..14448)
                     /gene="trpD"
                     /locus_tag="PLT63_04715"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HOD98061.1"
                     /translation="MIKEAIGKVVGGADLREGEMVATMTEIMEGQATPAQIGAFMTAL
                     RLKGETSEEVTGAARVMRQKATRIDARSSTIIDTCGTGGDGRNTFNISTTAAFVVAAA
                     GLTVAKHGNRAVSSGCGSADVLEALGVNIDAQPEIVEECVQQLGIGFLFAPKLHGAMK
                     YAIGPRREIGIRTIFNMLGPLTNPAGATCQLIGVYDAKLTEMFATVLKNLGTKRAFIV
                     HGADGLDEATITGETRVAELREGEVIAYNLDPTDLFGDVVPDADLRGGDAAANARITT
                     DILTGMDRGIRRRIVVLNAALAIVAGGRAESLREGIEVAEACIDEGGARRKLQGLIEM
                     SNS"
     gene            complement(14569..15165)
                     /locus_tag="PLT63_04720"
     CDS             complement(14569..15165)
                     /locus_tag="PLT63_04720"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HOD98062.1"
                     /translation="MILMIDNYDSFTYNLVQYLKQMGREVDVYRNDEFTAADMAGRDI
                     EAIFLSPGPCSPREAGVTVDVIRHFHQDIPIMGVCLGHQAIGYAFGGEVVRAAKVMHG
                     KTSSIFSDGRTIFRGLPNPFTAGRYHSLIVRRDNLPACLEISAETEEGEIMGLRHREF
                     PVEGIQFHPESILTPQGKRIIRNFLNLTEERKNRAQAA"
     gene            complement(15178..16683)
                     /gene="trpE"
                     /locus_tag="PLT63_04725"
     CDS             complement(15178..16683)
                     /gene="trpE"
                     /locus_tag="PLT63_04725"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HOD98063.1"
                     /translation="MYYPDLETFKKLTREGNLIPVYREILADVETPVTALSKLAAKSH
                     VFLLESVEGNEKWGRFSFLGADPQLIFRVSGAEVIISNNGSIRRFRHGGDPLRHLQAL
                     LQKYRPIAVTGLPRFYGGAVGYLGYGMVRYFEKLPVRAADDLGTDEAVFLLTDTILVF
                     DNVRHTIKIVACAFVDEKDGVTPEASYREATAKIDRIMEDLQERAPSLPPAGPAPADG
                     EIAISPNMTAEAFREMVRKAKDYILNGDIIQAVLSQRFAVENSFAPLHLYRALRFINP
                     SPYLFFFKAGETILIGSSPEVLVRLEEGVAELRPIAGTRRRGGTEQEDRKMADELLQD
                     PKEKAEHVMLVDLGRNDLGRIARTGSVQVNQLMAVERYSHVMHLVSHIQAQLEAGKDA
                     FDVIRAAFPAGTLSGAPKIRAMEIIEELEPHQRGPYGGAVGYFSYTGNMDFGITIRTM
                     LLKNNRIYIQAGAGIVADSDPETEYRETQSKAAGMLRAIRLAASNFELMDL"
     gene            complement(17012..17929)
                     /locus_tag="PLT63_04730"
     CDS             complement(17012..17929)
                     /locus_tag="PLT63_04730"
                     /inference="COORDINATES: protein motif:HMM:NF012799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOD98064.1"
                     /translation="MSKKNKMRIIIQCVMIFLFFLSGVSMGATGKFTIVGMGTTPDLM
                     TIRAVECVKEADIVILAEAGDKTAWKELIARKEVWFLGHNTQVFYGVDPETLKDQAAR
                     EKAFRLDKQRREMIDRITAAVRKGKKVAFLQGGDPMIYGITYPLEMLPKSVPVEIVPG
                     VGAFQAASAAVKMSPPYGWDTSSVILTMNDWTGHADTNEKLMATQTSMVFYTMHLNYP
                     ELFAQLNRHYPDNTPVAVVNHAGDRTRQKVIMSTVGRFLDEVNYRELPLEAHLLMVGK
                     FLKTGQARKEGLVGGKSYIEQRYGSGGHE"
     gene            complement(17989..18705)
                     /locus_tag="PLT63_04735"
     CDS             complement(17989..18705)
                     /locus_tag="PLT63_04735"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOD98065.1"
                     /translation="MNYQLRALGISGLLHIILVATVVIAGSLLEQGKKTIVLDFELRE
                     PTQEITKPELPARLSEAKPVPPALREHARPTGTPRMQAAPKSSTNPETLATPELSEAE
                     RRENQPLELGVSGLEKEEKGNYVGIARGTDPNTVRTDGSKKASKIKYLNDHFAYIRDK
                     ILRNVRYPDAARRMGWQGKVVFSFVIKEDGFVGRFQILQSSGFPLLDKSAMETVKETA
                     PFPRPPGEAQLVIPITYRLD"
     gene            complement(18702..19082)
                     /locus_tag="PLT63_04740"
     CDS             complement(18702..19082)
                     /locus_tag="PLT63_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOD98066.1"
                     /translation="MEEKEFDYINVIPLVDVMLVLLTIVLTTSSFIASGMIPLDLPKA
                     QRSAGEILKTQTIEIIGDGRIFLNSRPVSLAELKAELAGRNRKAPVLIRADRRINLQN
                     FVSVLDVVKGLDFSRVTLQTQEGT"
     gene            complement(19063..19488)
                     /gene="exbB"
                     /locus_tag="PLT63_04745"
     CDS             complement(19063..19488)
                     /gene="exbB"
                     /locus_tag="PLT63_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HOD98067.1"
                     /translation="MGVFVDYGIIGFLILLSVIAIGIAIERFYFFRHVKLGTFTDRKS
                     LEIELTRKIHLVATIGSNAPYIGLLGTVLGIMATFYTIGRDGFMDTGKIMVGLALALK
                     ATAIGLLVAIPSVALYNFLLRDIKVLLNRWEREHGRKRV"
BASE COUNT         3874 a         5535 c         5386 g         4633 t
ORIGIN      
        1 tgtccccctg gaccagggac agggtgccct tgttgacctt cacttccata acgattacct
       61 cccggccata gagatcttta ccgccaatgg aaaacctgtc cgctggcgga aaaataattt
      121 ttccagatat ccctttattg tatgagggat tgataagaaa tctaacccgc tgtgccgccg
      181 gatgcaagaa aaaaacgccg gaaaaacaat ataaccctta ggtgctgccg gtattagggc
      241 gggcaggaaa aatcgtggat ggttacccct atccgtccga taacgatgct caactggata
      301 agagccgacc agtcctgtcc gtcggccagc ttgatatggg tggcgtctac ccccacctga
      361 ttgaaggtgg gatcgacggc aatccatccg tccagatagc tttccgccca ggcatgatag
      421 agaaatccca tcccctccat gtagacgatg ccgccgacaa cccggctcgg tattccggcg
      481 gccctggcca tggctgtgta aagcatcgtg tgggctttgc attcgccccg gcggttgtgg
      541 aggacttcaa gcgccgaata actgtcaacc gcctcgtccc gcacttccgc agccacccag
      601 ccggccaggc gcacgacttt ttcccggggg gtgcgggtcc ccgagacaac ggccgccgct
      661 gcttttctga tttccgggct gtcgctctcg atgtggccgg agggcgcaag gtagcggctg
      721 cggtcccgga gcggcagggc gcttttccgc aggtctgccg gcatgggcgc cgttttccga
      781 atccggtata ttctcaccgt tttcccctcg tgcagtgttt ccacaacttc ctgataagac
      841 tcccggagcg ggggcagcaa tccggaaatc cctgcgacgg cggcttccaa agtggtggct
      901 gttctgggac aggttatcgg tttgtcggtc ttgatcacgc tgaagtcgta aatcagatct
      961 ttcttgctca gacttgcttc ggccaggaat gacttcgcat tttcttcact ttccttgaag
     1021 gtgatcagga ccccgctcat gccgagctcg aagattgttt ccccccggca gttgatccaa
     1081 ctggagacgg tatggcccag catgccggtt tccaccttga aagcgggttc gagatccagt
     1141 tcccggctct cttcgaaaga tacaatcgac tgggatactc ccgcgatgga ctgagtctgc
     1201 ggatcgtaaa ccgaatattt ataaccggtt cccaatttca taccctgaag gaccggataa
     1261 aggttgatga cactgctggg atataaaggg gagtcaagaa ttgtctcaat ctttttagtc
     1321 accccgcccg atcgctgctc tacagacagg ctgccggcag cgacggcgcc ggtgaggatg
     1381 agtttctttt ctcccacgtc ttgttcataa agaaatgata ccagggtgag atcgggattg
     1441 acgatatcca cgctcttcat gataattttc ttattcaggc cgagaaagcg gatacgcagg
     1501 tttgcctccg cggtgacccg gtaggttatg gctcccgcga ccggttcgat cttcatatgc
     1561 aggaatccca ccttttcgcc attgaagaca aagccatgcc aggattcacg ttcgggaaga
     1621 tttcccagtt ccttgatttg gcatggatcc gtcgggggcg aagcaggtcg gaagtagcgg
     1681 gccgtgcacc cgttgatcag catcgtggcc atgacgatga ccaggatgag atgcagatag
     1741 ccggcaggct gcccggagca ctttgcggcg ggcgggattt tcgtgatggt aagcaagcgc
     1801 ttcccttgct gtggataatt gatcatgcct gacggaaatc tcaaaacaaa agtaccctcc
     1861 ggcaaacccg ttcgcaaccg tgaggatctt aaaaagaaaa cacgttcatt catgagaacc
     1921 ctgctgaaaa gacccgctca tgtcaaaagc tacttcaaac atcccaaggt gctctatgcc
     1981 gcataatgaa attcatatgt tcaattgccg gattaataat ttctcttcaa ggcctcaacg
     2041 ctggcgatta ttttctgcaa agacgatccc ggaccgaaca ccgcggcgat ccccgctttt
     2101 ttcaaaatcg gcacatcgtc cggcggcacg gtgccgccga atatcacctt gatgtcctgc
     2161 gccttttctt ttttcagcag tttcacgact tccggcgcca acatcaggtg gttgccggaa
     2221 agggaactca gcccgatgat gtccacgtct tcctgcagcg ccattttgac gatgttggcg
     2281 ggcatttgat tgcccccgta aatcacttcc atccccgcat cgcgcaaggc cctggccaca
     2341 acgatacttc cccgccagtg ggcatccatc cccagacgga ccatcaatat gcgaatcctt
     2401 tgttttttca ttatctcgct ccctgatatc atttttttta aaaaagtcgt taaaaaagag
     2461 gcggctccca gaccccgtaa acacgtttca gcgtcctgga aatttcacct tccgtaagga
     2521 aatttttgac ttcacctacc atcagtttca tcagattctt gtttctttcg caacagccgg
     2581 cgatggcatc cagagatttg gccacggccc cggcatttct cgtttttttg attttcttca
     2641 gttttcttgt ctgcaccttt acggtttccg gtgaagagaa aagctcgatc ggcagcactt
     2701 cgtcctcgat ctggtagcag ttcacgccga cgatcggcat cgcgcccgat tccacatcca
     2761 actgatattt ataggcggcg gccgcgatct ctccgtgtat ccagcccttt tccacggcct
     2821 tcaccaaacc cccgatccga tcgatatcgt ccaggatcct gagcacttcc gcttccatgc
     2881 tgttggtaag ggattccaga taatatgaac cgcccaaggg gtcgatggtg ttggtaactc
     2941 ccgtttcgtt ccggagtatt tgctgggtgc gcaatgctgt taccgatgag agttccgtgg
     3001 gcgtgcataa ggcttcatcg taggcgtcca catggacaga ctgggcgccg cccagcacgg
     3061 cggcaagggc atggtaggcg gagcgggcga tgttgttcag cggctgttgg gcggtgaggg
     3121 ccacgccggc agtctgcacg tgaaaccgga acatgagcga gcgcggatcc tgggggtgat
     3181 atcttttctt catgacatgg tagtagaccc tgcgggcggc ccggtatttg gcgatttctt
     3241 caaagaagtc gttgtgggcg gagaggaaaa agctgagtcg tttcgaaaag ttgtctatct
     3301 tcagaccccg ggcgagcata tcctcggcac aggccagcgc gtgagcgatc acgaaagcga
     3361 tttcctgcgg cgctgtgcaa cctgcttcgc gataattata tccggcaaaa ctgatgggat
     3421 tccattgcgg agcgtatttt gtacagaact cgatggcgtc gcaactgatt ttgaaagaag
     3481 ctctgggggg cagcaccccc ggggcgatgg tggttgccgt ttccatcaaa aaatcatttt
     3541 ggcttgttcc ggccaagttc gcgagcggaa tgccgcggtc gagagcgttg gcaaaataca
     3601 tggattgcac ggaaatattg cagatcggct ggcaggttac cagcgagacg cttaccttgt
     3661 cgatgggaat atcatcgaaa agggtgcgca tgtcgccgat cgtatcaatc gccaccccgc
     3721 cccggccgac gtcggcgtaa acatcgggat cgtcgctgct gtagccccgc agcgtgggat
     3781 aatcgaaaac cccgtttatc ccggtggcgc cctgctggag caggaacttg tagcgcttgt
     3841 tggtttcctc gggcggaccg aatcccgcca gttgccggtg cgtccaggtc cggccgcgat
     3901 acatcgtggg atgaactccc cggatgaacg gccccttccc cggatacccc aggtcgcgga
     3961 gataatccag gccggccacg tcatccggcg tgtaaagagg ctttaccttg aatccggata
     4021 atgtttcata cttgtctttc agccgttgct ggcatgaata cgagttctcc tcccaatccg
     4081 cggtttccct ggcgacctct ttaatcctgt tttgattaaa aatgcccgtt ttcacgatag
     4141 accctccttt gcataatttg agcgtctttt ttgcacataa tatgccaggg atttttcagg
     4201 acagtgtttc ccgcggggaa ggtcagaact ttattccgga tcagtatttt atgctgtctt
     4261 cagctatgct cacgcacttg aaggtatcct tagggtaggg cttttgtgaa atatttttac
     4321 atcggcggtg aaattatttc acttgtccgg cagccggtgc ttattcatta gataccgcag
     4381 tgaaaaacga tccaaacgca gcagttttgc cgcttccgtc ttgttccctc cggtcttgag
     4441 cagcgcctgc ttgatcagtt tgctctgtaa tgccgacgtg agttccttga tgtccaatcc
     4501 ggaatccggc aggttaattt ccgctgctcc ggttacgggg tctgcatagc tctcatcggt
     4561 gatttcccgc ggcaagtgct tgagcaaaat aatctcggga ttgtcgatga tcatgatccg
     4621 ctccatcgtg ttccttagct cccggacgtt tccgggccag gaataattca acatggcggt
     4681 gattgcgtct tcgcttatgc ccttcacatt ctttctgtag tcgttattga ggagatcaag
     4741 gaaatatctc gcggtcggaa cgatatcctc ctttcgctcc cgtagcggcg ggataaaaat
     4801 tgtcaggacg tttaagcggt aatacaggtc ctcgcggaat ttattctggc ggattcgctc
     4861 cttgagattg tggttggtcg cggcaattat tcgggtgttt acctgaatcg tccgctgccc
     4921 acccaggcgg cgaaacctct tttcctgcag aacgcttaac agtttcgact gtatgatcag
     4981 gtccatatcg ccgatttcat cgagaaacag tgttcctccg gaggattgtt caaaaaggcc
     5041 tttcctggca cgcttggcat cggtaaacgc cccctgctca taaccgaaca actcactttc
     5101 caggagggaa tacgggatag ccgagcagtt gatctcgatg tatggcccgt tcagcctggg
     5161 gctgccgtaa tgaatcgctt ttgccaccag ttctttgccg gttccgcttt caccatggat
     5221 cagtactgtt gacgatccta tttgcgagac cttcgcgatc gtctcctgga tgtcctgcat
     5281 ggcccggctc tgaccgatga tcctatccgg tttgaacagc gatctgttcc tgtctctgag
     5341 ttcgttaact tccgtctgca gcttttgggt ttccagtgcc agctttatta tggtggagat
     5401 gtgcctggat ttgaacggct tgtttatgta atcaaaagca ccgagcttca ttgcggccac
     5461 ggcgttttcg atggacccgt agccggtcat cattattacg gtgcattctg ggtttaaggc
     5521 cttgatctgc ttcagaacgt ctatgccgtt gctgtccggc agtttgagat ccagcaggac
     5581 aagatcgaca cttctttttt tcaggacggc aatgccctgt ttgccgtttt cggcggacag
     5641 gacatcataa ccttcgtcgc tcaatgtcag tttcagggaa tctctaatga gcttctggtc
     5701 gtctatgatc aggatggtat tcatattccg ccccgcttat gcatccaggt attcaacagc
     5761 ttttctgcag tcacagggca ggattatcgt aaattccgtc cctttgccaa gttctgaatc
     5821 gaccttcaca atcccgctat gctccttgat tatgctgtgc gttatggaca gccccagccc
     5881 cgttccttca ttgtttgtcg tgaagaatgg atcgaagata cggccgatat tttccgttgc
     5941 gatacccacg ccggtgtcgc cgatggttat ctcgatgcgt ctctccgggt cgccggagcc
     6001 ggaatcgccg gttagttcgg cggcctttat ggtgagattt ccacctgacg gcatggcctt
     6061 tatcgcattg atgacaatat tcagtaatcc ttgtctgatc cgctcctgat ccacgtacag
     6121 gccgtctatg tcatcgtcaa ttctgatgaa catttttatg ccgcggtgat tgcactgctc
     6181 gtttatgagc agatagatac tgttgataat attcctgatg tcggcaagtt cgaagttgaa
     6241 ctttttagga cgggcgaagg tgagcagatc ggaaatcagt ttttccagcc gctctatttc
     6301 cccatttgcc tcattgataa gttcaaggtc cctccggggc agtttcttgc ggctcaagaa
     6361 atccagcacc acgccgatcc cggtcaaggg gttgcgaatc tcatgcgcta tgccggcggc
     6421 aagctcgcct aatgatatga ggcggtttac ccgctgcagg tactcgtcgc gtttcctttc
     6481 cccggtgatg tcctggatga gaaacatgaa gccgataagg ctttcccctt cgttcatgat
     6541 atgggaagtg gtgacattta aaatcttgtc atccggattc agggtgaatt ccatcccttt
     6601 catgttgtgc agatgaaaga cagctttcct tgtgcattca atccattccg gcagggggaa
     6661 aatctcccag ataaatctgt tgattaaagt atccttcatc atgccgaaca ttttttccac
     6721 agcggggttc agggaggtta tccttcccgt aatatctacc gtcagaatgc cgttgatcga
     6781 gctgtttaaa acattttccg agaatttttt ctcgttcatc aaatcctgca acagtttcga
     6841 attttcgatg atgatgctcg cataattggc taaaatggat agtgactcga attctttctc
     6901 gtttatttcc ggctccccgt gtttcttatc gacgcccaga atgccgatga tgtccccttt
     6961 gatcttgaga ggcacataca atgtgcatcc gcgacccatc cgggtggtga ccctgcggtc
     7021 gagatccgtc aaattgtctg atgaactgaa attcttcact aataccggtt cgccggtgac
     7081 ggctactctt gtttctatgc aatcgtgctt cttaatgtcg aaaggtcttt cctttacgta
     7141 gttctcggtt tccggggaaa agccggtaat gtacttgcat tcaagcttgt ggttcgtctt
     7201 gtttaaaaga tagactgaac atctgttgaa gttgaggtcc tgtgtcaccg tctgcactat
     7261 cgcttcgacg accctatcca ttgtcgccgg ctcggtcagc agcgaactta ttctgtatac
     7321 atgggataag atctggtcgg tgaattcgta actcataaca tataaaaatg actggtgacg
     7381 ttatcaatta acttaattcg tggccgggcg ggagttggcg gcattgacga tggcctccgt
     7441 ttcttcccgg gtgatgtccc cctggaccag ggacagggtg cccttgttga ccttcacttc
     7501 cataacgatc acctcccggc cgcttatttt atcgttctgc tcttttggga ttttcccgcc
     7561 ctatttaaca caagcggtga ttcgatttca atcctgcgcc tgtcaatgcg gccagggctt
     7621 aacctttaca tctttcaccg cttccgccca cccgcatggc ggagaacgcg aagacagctt
     7681 ataaatcaga ccgcgggttg ctgatagacg gcaaattgta aaaaggcgcc ggtaatcttg
     7741 ctcaaatact tgtccttacg gtaggcaaaa tagaaggttc ttttaagatc gacgccggaa
     7801 acacgcaacg actttatgac gcccaggtat tcctccctaa ggacggaggc cttcgagaga
     7861 atggaaaccc cgagacccgc ttccaccgcc ttcttgaccg cctccgtatt gccgatctcc
     7921 agcgtgtttc tcaaaataat gccggtggcg gcaagctttt tttcaatgat atctctcgtc
     7981 cccgatccct tacgtgggta caggaacgtc acccccagca ggtcgtgtgc gtccacggcg
     8041 tcccacaatg accattcgtg cgtggcggga acgataagaa ccagctcatc ctgcctgaac
     8101 ggctccattt ttaacctttc atcattgacc ggtgccccca gaaacccgac atctgtggaa
     8161 ttggcgatca ggtttcccac cacctgtttg ttcatagcaa tatccagatt gatattaacc
     8221 cggggatacc gggaacgaaa ccgtcccaga attgccggga ggatataaat gcccagggtg
     8281 ttgctggcgc ccacattgag cacgccccgg tccatgccct gcagttcatc gatctcaatc
     8341 ttcatctgct cgattatgcc gaagatgatc tctgttcgtg cgtagaggat ctcgccggcc
     8401 tgggtaagca caaccttttt ccccaggcgg tcaaagaggc gtgtcccgta gtactcctcc
     8461 aggtctttaa tatgcttgct gatccccggc tgggtaagga acagggcttc cgccgcccgg
     8521 gtgaagctct ggactttagc cgccacatga aaaaccttca attgattgag gcttgcttcc
     8581 ataattatac attatgctac aaataattat tattcagttg aattatatgc aggatgacct
     8641 tatttagcaa acaaaaagat tgttgaggat cctaagccat ggacaatgcc gacagggaaa
     8701 agatcgaaaa gatcatcagc gacagaaaag cggcccacgc attctacgag gacaactcgc
     8761 agacttatgt tacctttgcc aggatggcaa atgaggcgtt tgcggcaggc gaactgggca
     8821 ggaaaacacc tgaaggtcat caattactat caagaaaaga ataaccgccg ctaacgaagg
     8881 cggcacaaca aaattatgga ggaggttgcg tcatgctgga taaaacagat accgacaaga
     8941 ttgaaaaggt tctcggtgat ttaaaatacg tccacaagtc tttcaccttc agggattcca
     9001 agtgctatgg gcactatatg cagcttctga atcatgccat ggtggacaga agtctgacga
     9061 gaatgaccaa ggaactgatc gccgtcggca tctccgctta tgccaactgc gagccctgca
     9121 tgaccgttca tgtccgggag gcgctgcgcg ccggggcgac cgatgagcaa gtggaggagg
     9181 ccgtcgatgt ggccatggaa atgggcggcg gacccgtagt ggcgaggggt ggcgcctaca
     9241 tattcaaact gctcgaatac ttccgaggta aaggcaggta ataacgtcgg cattggcggt
     9301 gatgaccgtg agggggagcc gtgaagcgtc tcagtccggg gggcagcctc cctgcgtgcg
     9361 ccatggacag ggggagtcat gattgccggc ggcaatcgcc cccggcgcat ccgcagtcgg
     9421 gagcgccggg gatatgcgta tctccggtaa tcccggttat ggtccggttg ccgccttgag
     9481 ctcccggacg taggcggcgg cctggggaag caggtccggg tcctcactgt gcgtggcgag
     9541 ccatttgacc agggcgctgc cgatgaccac cccgtcggcc tgagcggcga agcccctcac
     9601 ctgggccggc gtggagatgc cgaaacccac cgctacggga agatcggtcc ggcgcctgat
     9661 ccgctggagg tcgggaagca ccgccgcggc ctccggggtc ttcgtccccg tgaccccggt
     9721 tacggagatg taatagatga atcccgcggc gtccttgaca atcccggcca gccgccggtc
     9781 gccggtggtc ggggcgacca gggtgatgaa gtccaggccc cggggatcgg tgaattgccg
     9841 caactccgcc gcctcctcgg ggggcaggtc tacgacaagg agtccatcca cccccgccgc
     9901 cgcggcctgc cgggcgaacc gctcgttgcc gtaagcgaag acgggattgt aataggtgaa
     9961 aaggacgatg ggaatatccg acccccggcg gagacgggcg atcatgtcca tgatggcggc
    10021 gagattggtc cccgcccgga gggagcgttg ggccgcctcc tggatgaccg gaccgtccgc
    10081 tgtaggatcg gagaagggaa cgccgatctc caggatgtcc gccccggcct catccagaat
    10141 ggggatcagc gcctccgtcc tttgcaggtc cggatcgccg gcggtgagat agaggatcag
    10201 ggccttttcc ccccggctcc gcaaggcttg gaatcttttt tcgatgcggt tcatggcagt
    10261 tccacctcct tttgtgcggc aacgatgccc atgtccttgt ccccccggcc ggagaggcaa
    10321 acgacaatga tcttgtcccg gccgagcgcc ggggccagct tcagagtgta ggcgagggca
    10381 tgagcgcttt ccatggcggg catgatcccc tcacaggtcg tcaggagccg gaaggctgcc
    10441 agggcctccg cgtcggtgac ggataggtac cgagcccgtc ccgcggcatg gaagaaactg
    10501 tgctccggtc cgacgccggg atagtcgagg ccggcggaga tggagtgggc gtcccgcacc
    10561 tgtccgtgct cgtcctggag gaggtaggtc ttgttgccgt ggagaacccc gactgcgccg
    10621 gcggagatgc tggcggcatg gagacccgta gggatgccct tgcccccggc ttccacgccg
    10681 atcatggccg tgcccccgtc ctggcggaag ggataaaaca gccccatggc gttgctgccg
    10741 ccgccgacgc aggccacgag aaaatcaggg aggcgtccct cctttttgag gatctgccgg
    10801 cgggcctccc ggccgatgac gctttggaag tcccggacca tacgcggata ggggtggggg
    10861 ccggcggtgg tgccgatgac gtagaaggtg tcccggaccc gggctaccca gtggcgcatg
    10921 gcctcgttca tggcgtcctt cagggtggcc gttccggagg caaccggggt cacccgcgcc
    10981 cccagcattt ccatccggaa gacgttgggg gcttggcgcc ggatgtcctc ctcccccatg
    11041 aagacctcgc actccatgcc gaagagggcg gcgacggtgg cagtggccac gccgtgctgg
    11101 ccggcgccgg tttcagcgat gacccgtttc tttcccatcc gccgggccag gagagcctgc
    11161 cccagggtgt tgttgatctt gtgggccccg gtatggttga gatcctcccg tttgagatag
    11221 atcttggccc ctccggcctt tgccgtgaaa ttttccgcaa aatagagggg tgtctcccgg
    11281 ccggcgtaat catggaggta gtggcggaat tccctccgaa actccgggtc ctgggccgcc
    11341 cggcggtagg cctcctcaag ttcgatcaag gcgggcatga gggtctcggc gacatagcgg
    11401 cccccgaaga tcccgaagtg cccctgtttg tccggcaggt tctttttcat atgctcctct
    11461 tgcagttaaa tgtttgtaat tacgtaatta tgtgaaatat atctccggtg gaccggatat
    11521 tacaattgtt gagtcatccc cctcctcgcc tccccctgcc gggggagaaa cgaaattgcc
    11581 cttcacgggg gaggggaaat catttttttt acgagcttgt cgctctcggg gaggaaaacg
    11641 gtccgttccg gcaaaggaag atggcgtccc ggctttcccc gtttttcagt tcgtcttctt
    11701 cccgaatgag gcggatgatc tccgccacct tggccggatc cttcaccccc ggcgccgtct
    11761 ccacaccgct gttgatatcc annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11881 naccttggcc ggatccttca cccccggcgc cgtctccaca ccgctgttga tatccacggc
    11941 tcggggccgg accgtccgca gggcggcatg gatgttttcg ccgttgagcc ctccggccag
    12001 gatcaaccgc ccggttttcc cgagccgggc agccagctcc caggaggctg atttccccgt
    12061 tcccccgtaa cggtcgtcgt cccgggcatc tacgaggatc gccgtcaccg ggtaaagggc
    12121 cgcccggtgg agatccgccg ccgttgtcag ggggagggcc ttgatcagga ggttgcggtc
    12181 ggcgatgccg cggcagtagg ccggactttc atcgccgtga agctggagca tatccagacc
    12241 gcaggtcgcc gccgtctccc tgatcaccgc tgcctcctcg ttgacgaaga ccccgacctt
    12301 gcagacccgg gcggggaggc gggcgatgat tgccgccgcg gccgctgggg agatgccgcg
    12361 gggacttttc cggtaaaaga tgaatcccag ggcgtcggcc ccgcaggcgg cggcgtgtgc
    12421 ggcgtcttcc gggcgggtga tgccgcagat tttgatttcg gtcatggccg gtcttcctct
    12481 ccccgcagct cccgcagttt tcttcccatg tcttccgacc gcatcagggt ctcgccgacc
    12541 agcaaggcgt cataaccggc ctgccgcagc cgctccacat cccggcggcc ggtgatgccg
    12601 ctttcgctca ccttgacgac ccccgccggg atcagcgggg ccaggcgcag ggaggttccg
    12661 aggtcggtga cgaaggtggc gagattgcgg ttgttgaccc cgatcgccgg cgccccggcg
    12721 gccaggctcc gtgccagttc ctcttcgtcg tgcacctcca ccagcggtgt catgcccagc
    12781 tcccgggtca aggcgatgag ctccgccagg atggcgccgg gcagggcccg ggcgatcagg
    12841 aggagggcgt cgccgccgag gcagcgcgtt tcgtaaatct ggtatggatc gacaaggaag
    12901 tcctttctca gcagcgggat gcgcaccgtc ttcctgattt ccgcaaggta gcggccatcg
    12961 ccctgaaaga aggaccgttc cgtgaggacc gagatcgccg ccgcgccatg ggcctcgtat
    13021 atcccggcga tggcgtgggg atcgaaatcc tcccggatga ccccccggga cggcgaggcc
    13081 tttttaatct ccgcgatgat cgcacattcc ctgcccgtca gggcttcctg aaaatttcgg
    13141 ggcggcggaa cttcgcaaag gatggcctgc atctcccgca gcggtgccag ccttttcgtt
    13201 tccgcaagct ccgcttcttt tgccgccaca atcttttcca aaaaattatt ttccattgcc
    13261 gtcctgtttg atgaaccagt aaagagtaaa atattggcta taattgacgg cagcgtaaaa
    13321 agatccgcag gcaaggcgcg caagtcctga ggactgaggc gtacttggcg tacgccgcag
    13381 ggacgaagga tgcagcgcaa cgccgcatcc ggactttttc cgctgccgtc ctgctcaact
    13441 gttggacatt tcgatcagcc cctggagctt ccggcgggcc cctccctcgt cgatgcatgc
    13501 ctccgccacc tcgatgccct cccggaggct ttccgccctc cctccggcga cgatggccag
    13561 ggcggcattc aggacgacaa tccggcggcg gattcctctg tccattccgg tcaatatatc
    13621 ggtggttatc cgggcgttgg cggcagcatc gccgccccgg agatcggcgt ccgggacgac
    13681 atccccaaac aggtccgtcg gatccagatt gtaggcgatg acctccccct cccggagttc
    13741 cgccacccgg gtctcgccgg tgatcgtcgc ctcgtcgaga ccgtcggcgc cgtggacgat
    13801 gaaggcccgc ttggtcccca gatttttcag gacggtggcg aacatctccg taagctttgc
    13861 gtcatagaca ccgatgagtt ggcaggtggc cccggcggga ttggtcaggg gacccagcat
    13921 attgaaaatg gtccggatac cgatctcccg gcgggggccg atggcgtact tcatggcgcc
    13981 atggagcttc ggggcgaaga ggaaaccgat gcccaactgc tggacgcatt cctctacgat
    14041 ctccggctgg gcgtcgatat ttacccccag ggcctccagg acgtcggcgc tgccgcagcc
    14101 gctggatacg gcccggttgc cgtgtttggc cacggtcaga cctgccgccg ccacgacgaa
    14161 ggcggcagtg gtggagatgt tgaaggtgtt ccggccgtcc ccgccggtgc cgcaggtatc
    14221 gatgatggta gaggaccggg cgtcgatgcg ggtggccttc tgccgcatca cccgggcggc
    14281 gccggtgacc tcctcgctgg tttctccctt caggcgcagg gccgtcatga aggccccgat
    14341 ctgggccggc gtggcctggc cttccatgat ctccgtcatt gtggcgacca tttcaccctc
    14401 ccgcaggtcg gcacccccta ctaccttgcc tatcgcttcc ttgatcatta tgttctcctt
    14461 ctcaagcaaa tgcttggctg tatttactga gtttcaatat tgacggcatc gtaaaaaatc
    14521 acttttccgc cccccttcaa taagggatgg gaatagggcc gcaataaatc acgctgcctg
    14581 ggcgcggttc ttcctttctt ccgtcagatt caggaaattg cggataatcc gcttgccctg
    14641 gggggtgagg atcgattcgg gatggaactg tatcccctcc acggggaact cacgatggcg
    14701 gagccccatg atctccccct cctctgtctc cgcgctgatt tccagacagg ccggcagatt
    14761 gtcccgccgg acgatcagag agtggtaacg cccggcggtg aagggattgg gcagccctcg
    14821 gaagatcgtt cgcccgtcgc tgaagatgga agaggtcttg ccgtgcatga cctttgccgc
    14881 ccggaccacc tcgccgccga aggcatagcc gatggcttgg tgcccgaggc agacgcccat
    14941 gatggggatg tcctgatgaa agtggcggat gacatccacc gtgacgccgg cttcccgggg
    15001 agagcaggga cccggggaaa gaaagatggc ctcgatgtcc cggccggcca tatccgccgc
    15061 cgtaaattcg tcattgcggt aaacgtccac ctcccgtccc atctgcttca gatattggac
    15121 gaggttgtag gtaaaggaat cgtagttgtc gatcataagt atcatggtta gatctcctta
    15181 taaatccatc agttcgaagt ttgacgccgc caggcggatc gcccgaagca tcccggccgc
    15241 cttgctctgc gtctcccggt attccgtctc cgggtcggag tcggcgacga tgcccgcccc
    15301 ggcctggatg tatatccggt tgtttttcaa cagcatggtg cggatggtga tcccgaaatc
    15361 catgttgccc gtatagctga aataacccac ggcgccgccg taggggccgc gctggtgggg
    15421 ttcgagctct tcgatgatct ccatcgcccg gattttgggg gccccgctga gcgtgccggc
    15481 ggggaaagcg gcccggatca cgtcgaacgc gtccttcccg gcttccagtt gggcctggat
    15541 atgggagacc agatgcatga cgtgggagta gcgctccacc gccatgagct gattcacctg
    15601 gacgctgccc gtccgggcga tgcgtcccag atcgttcctc cccagatcga ccagcatgac
    15661 gtgttcggcc ttttccttcg ggtcctggag gagttcgtcg gccattttcc ggtcttcctg
    15721 ttccgttcct ccccggcggc gggtgccggc aatgggacgc agctccgcga ccccctcttc
    15781 caggcggacc agcacttccg gcgaagagcc gatgagaatc gtctccccgg ccttgaaaaa
    15841 gaaaaggtac ggcgatgggt tgatgaaacg gagggcccgg tagaggtgga gaggggcgaa
    15901 gctgttttcc accgcgaagc gctgggaaag gaccgcctgg atgatatccc cgttgaggat
    15961 gtagtctttg gccttgcgga ccatttcccg gaaggcttcc gccgtcatat tggggctgat
    16021 ggcaatttcc ccgtctgccg gcgccggtcc ggcgggaggc agggatggcg ctctttcctg
    16081 cagatcttcc atgatccggt cgatcttggc cgtcgcctcc cggtagcttg cctccggggt
    16141 aacgccgtct ttttcatcca caaaggcgca ggccacgatc ttgatggtgt ggcggacgtt
    16201 gtcgaagacg agaatggtgt ccgtcagcag aaacacggcc tcgtcggtgc cgagatcgtc
    16261 tgcggcccgg acgggcagtt tctcgaaata gcggaccatg ccatagccca gatagccaac
    16321 ggcgccgccg taaaaccgcg gcagtcccgt caccgctatc gggcggtatt tttgcagcag
    16381 cgcctgcagg tgccgcaggg ggtcgccgcc gtgacgaaag cgccggatgc tgccgttgtt
    16441 gctgataatg acctctgcac cggagacgcg gaagatgagt tgcgggtcgg cgcccaaaaa
    16501 agagaaccgt ccccattttt cgttgccttc cacgctctcc agaagaaaaa cgtgggactt
    16561 cgcggccagc ttgctcaagg cggtaaccgg cgtctctacg tcggcgagga tttcccggta
    16621 aacggggatc aggtttccct cccgggtgag ctttttgaat gtctccaaat ccggatagta
    16681 catatgcctc ctgacaaaaa gaggccgtga tcgctcacgg cctctttcgt tgcggataaa
    16741 aaaaaaggcc gtggagtcct gggaagatct ccacggccct attggtttat gtcaagtgtt
    16801 tcaaccggcg gcgcgcagac ccctccccat gttctgccac caccaccagc cattgttgtt
    16861 caggaaagat tcgttgcgtt tcatttcaaa ctcgcttttc ctcgtttggg cgttgcttat
    16921 cagctttttt tccccttgtc aaaaactttt ttgccggttg tcttgtcaac gatcatgccg
    16981 gggagagcgg agagttctga catccgattt tttattcatg ccccccactg ccataccttt
    17041 gttctatata gctttttcct cccaccaatc cctcttttcg ggcctgcccg gtcttgagga
    17101 atttaccgac catcagcaga tgggcttcca ggggcaattc ccggtaatta acctcatcca
    17161 aaaagcggcc cacggtgctc atgatcacct tttgccgtgt ccggtccccg gcgtggttga
    17221 ccacggcaac cggggtgtta tcagggtaat ggcggttgag ttgcgcaaag agctccgggt
    17281 agttcaaatg catagtgtaa aaaaccatgg aggtttgggt tgccatcaac ttctcgtttg
    17341 tatcggcgtg gcccgtccag tcgttcatgg tcaggatgac ggaactggtg tcccatccat
    17401 agggaggact catcttaacc gccgcgctgg ccgcttgaaa ggccccgact ccgggaacga
    17461 tttctacggg aacgcttttt ggcaacatct ccaacggata ggttataccg tagatcatgg
    17521 gatccccccc ctgcaggaag gcgacttttt tccctttccg caccgccgcc gttattctat
    17581 cgatcatttc ccgccgctgt ttatcgagcc ggaaggcctt ttctctggct gcctggtctt
    17641 tcaacgtctc gggatccaca ccgtaaaaca cctgggtgtt atgtcccaaa aaccagactt
    17701 ccttgcgggc aatcagctct ttccaggccg ttttatcccc cgcctccgcg aggatgacga
    17761 tatccgcctc tttaacgcat tccaccgccc ggatggtcat cagatccggc gtggttccca
    17821 tgccgacgat cgtgaatttg ccggtcgccc ccatggaaac acccgacaag aagaaaagga
    17881 agatcatgac gcactggata attattcgca ttttattttt cttcgacatg ctgttgctcc
    17941 tttatttgat gaaattgcat aaatcccgga aaattttgcc ctccccgatc aatccagacg
    18001 atatgtaatg ggaatgacca gttgggcttc cccgggcggc ctgggaaatg gcgcggtttc
    18061 ctttacggtt tccatggcgc ttttatccag cagaggaaaa ccggaacttt gcaggatctg
    18121 aaatcttccc acaaaaccgt cctccttgat gacaaaggaa aacacgacct ttccttgcca
    18181 tcccatccgc ctggccgcgt cggggtagcg gacattgcgg agaattttat ctcgaatata
    18241 ggcgaagtga tcgtttaaat atttaatctt ggatgccttt ttcgagccgt cggtcctgac
    18301 tgtgttcgga tcggtccccc gggcaatgcc gacgtaatta cctttttcct ccttttccag
    18361 tcctgatact cccaattcca acggttggtt ttcccgcctt tcggcttccg aaagctccgg
    18421 ggtcgccagt gtttccggat tcgttgatga cttgggagct gcttgcatcc ggggtgtccc
    18481 cgttggtcgt gcgtgttccc gtaaagctgg tgggacgggt ttagcttcac tcagacgtgc
    18541 gggtagctcg ggcttggtaa tttcctgagt cggctcccgc agctcgaaat ccaacacgat
    18601 cgtttttttc ccctgttcca ggagcgaacc ggctatgacg acagtggcaa cgaggataat
    18661 gtgcagcaaa ccagatatcc ccaacgccct cagctggtaa ttcacgtgcc ctcctgggtc
    18721 tgtagagtga cacgggaaaa gtccaacccc ttgacgacat ccaatacgct gacgaaattc
    18781 tgtagattga tgcggcgatc ggccctgatc agaacgggcg cttttctgtt cctcccggcg
    18841 agttcggctt tcaattccgc gagagacacc ggccgggagt ttaaaaaaat tcgcccgtcc
    18901 ccgatgattt caatcgtttg cgttttaaga atttcaccgg cactccgctg tgctttgggc
    18961 agatccaggg gaatcatacc gctggcgata aaggaggagg tggtgagtac gatcgtgagc
    19021 agcacgagca ttacgtcaac cagggggatg acattgatgt aatcaaactc tttttcttcc
    19081 atgttccctc tcccatctgt tgagcaggac cttaatgtcg cgcaggagaa aattgtatag
    19141 agcaacggac ggaatggcga ccagaaggcc gatggccgtg gctttcaagg ccagtgccag
    19201 accgaccata atcttgcccg tatccataaa accatcccgt ccgatggtgt aaaaggtggc
    19261 catgatgccc agaaccgtac ccaaaaggcc aatgtacggc gcgttgctgc cgatggtggc
    19321 gacgaggtgg atttttctcg tcagctcgat ttccaggctc tttcgatccg tgaaagtgcc
    19381 gagttttaca tgtcgaaaga aataaaaccg ttcgatggcg atgccgatgg cgatcacgct
    19441 caagaggatc aaaaagccga tgatcccgta atcaacgaaa actcccatcc attccatatc
    19501 ttctcccgag atgtccgccg gtcatttt
//
LOCUS       DAOSMF010000027        18424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_3643_length_18424_cov_24.208830, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000027 DAOSMF010000000
VERSION     DAOSMF010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 18424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18424
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3643_length_18424_cov_24.208830"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..868)
                     /locus_tag="PLT63_04750"
     CDS             complement(8..868)
                     /locus_tag="PLT63_04750"
                     /inference="COORDINATES: protein motif:HMM:NF017652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HOD98068.1"
                     /translation="MSEPVIICAALTGAATFKQNNPSVPYTLEEFADEAEKCRRAGAA
                     IVHVHARTDDGDSTHEIDRVRAVHDAIKERCPDVIICLSSAVGAFKTPEQRLAQIVAV
                     RPEMASLNTNTMNFSIIDRNSGQIFFDYVFENTFTMLQDFGKAMETGGIKPEIECYDI
                     GGLDNALLISRQGFFSQPMNFNFVWGVAGGQKFRPEVMIAMKNALPPNANFTTCAVGP
                     EQFPANVLSCLLGGHMRVGMEDNTRMPNGDMAKGSFEQVEWAVRVAASLNRPPATPTE
                     ARKILGIRND"
     gene            complement(925..1086)
                     /locus_tag="PLT63_04755"
     CDS             complement(925..1086)
                     /locus_tag="PLT63_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98069.1"
                     /translation="MIDTIKDFILNYYYQVLSWYDGLTYVQQFFTLFGLFAAVGVAIG
                     LYLIKRAAS"
     gene            1380..2987
                     /locus_tag="PLT63_04760"
     CDS             1380..2987
                     /locus_tag="PLT63_04760"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOD98070.1"
                     /translation="MKVKFMGAARTVTGSCFIVEVGSHRFAVDCGMHQGNKEIEKRNW
                     DTTGYDPAGLEFILITHAHIDHAGLLPRIVRDGFRGPIYATPPTLELMKILLLDSAHI
                     QEMEAQWKTRKCLRYGETKVDPLYTQRDAEETARYFREVRYDQAVSPGDGMTVAFRDA
                     GHILGASILELTVTEDGKESRLVFSGDVGRPAQLIVNDPTVIETADYLFMESTYGNRD
                     HKDEDESLNELAEAIDFSYRQGEKTIIPSFAVERTQELLYCLHLLDRDGRLPKDMPVY
                     VDSPLATRATEIFRKYTKYFDEESKVLLKKGQDPLGFPQLRFTRSTEESMKINGAEGP
                     AVVISASGMANAGRIRHHLRHNLWRPGAAVVFVGFQAQGTTGRRIVDGAKSVRIFNED
                     VAVQARVFTINGFSAHAGQSQLLEWLGGFRNPGLRVFLVHGEYTAQEVLAGKIAARFG
                     YRVEIPDYLEQVTLEPGKEIEREVYPEQAKPGIDWEFLLGDLEQRLRQLRERRPRLEG
                     KGWVEQTDMRERLLELDHRLASLISEI"
     gene            complement(3002..3571)
                     /locus_tag="PLT63_04765"
     CDS             complement(3002..3571)
                     /locus_tag="PLT63_04765"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HOD98071.1"
                     /translation="MKTRRPLEKIAMKKAFLIALLVSFASHALVLSMPVMGNRIAPPP
                     PHPERSMTIELAESLPPEPEVREAPAGAGEKANRTTSEEQTVNLESPDRRYRSYLLKV
                     RQRIESRWSYPEAALKKGEQGIATVKFTIENRGVLSGQAVLTSSGSSVLDKHSLAVVR
                     AAAPYDRLPPELSLARLHIVASFHYRLKP"
     gene            3628..4131
                     /gene="tadA"
                     /locus_tag="PLT63_04770"
     CDS             3628..4131
                     /gene="tadA"
                     /locus_tag="PLT63_04770"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HOD98072.1"
                     /translation="MKIDHGAVPERGATGAPPEDDAFFMDLALAEAAAAAAAGEVPVG
                     AVIVLAGRVIARAGNRPISLKDPTAHAEILAIREAARITDNYRLPGTILYTTLEPCPM
                     CAGAMIHARVCRLVFGTRDPKGGAVVSAARMFDDYPGNHVVEYLGGVREESCAEILSS
                     FFMQKRL"
     gene            4166..4259
                     /locus_tag="PLT63_04775"
     tRNA            4166..4259
                     /locus_tag="PLT63_04775"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4201..4203,aa:Ser,seq:cga)
     gene            4573..4666
                     /locus_tag="PLT63_04780"
     tRNA            4573..4666
                     /locus_tag="PLT63_04780"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4607..4609,aa:Ser,seq:gga)
     gene            complement(4770..5150)
                     /locus_tag="PLT63_04785"
     CDS             complement(4770..5150)
                     /locus_tag="PLT63_04785"
                     /inference="COORDINATES: protein motif:HMM:NF018021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HOD98073.1"
                     /translation="MAMKLSSREFDRAVERAIGRIPAEIRDRLDNILISVQRRPSRQM
                     SMTLGLDPDDAPFGIYTGVSLAERSEFSPPLYPDTIFIFQEPLEDACRSIAELEEEIE
                     ITVVHEIAHFLGMTEEELAALGYA"
     gene            5432..6904
                     /locus_tag="PLT63_04790"
     CDS             5432..6904
                     /locus_tag="PLT63_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HOD98074.1"
                     /translation="MTSTRRTTAAVTFWAAIIFLAAAALPGEICIAAGEAGGHDIGRE
                     LPVWTATPFVGILLSIALFPLLAPHFWHRHFGKVSAFWALAFALPFLFFFRETALYEI
                     LHIMLIDYIPFIILLWGLFTIAGGIAVSGSLRGTPLMNSLLLLIGTVLASWVGTTGAA
                     MVMIRPVLRANKDRVKKAHIICFFIFLVANIGGSLTPLGDPPLFLGFLHNVPFFWVTT
                     SILPHMLLSSLILLAVFYAFDLFYYRREAPVPPATGERTPIRIEGLHNLLLLAGVIGV
                     ILLSGYWRPGHLSIFGIEVQYQNLLRDGSIVTLGLISLFVTPRRLREANDFSWGPILE
                     VAKLFAGIFITIIPALAILKAGSQGALAWLVDAVKTPAHYFWAAGILSSFLDNAPTYL
                     TFFNMALGRLDLTEAMIPTALAAGAATANPQFIAYLQAISAGAVFMGANTYIGNAPNF
                     MVKSIAEEAGVNMPSFFGYMLKYSIPILIPIFIAVSFVFF"
     gene            6920..7504
                     /locus_tag="PLT63_04795"
     CDS             6920..7504
                     /locus_tag="PLT63_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98075.1"
                     /translation="MSKDYYEVVVEGHPEYVNGFVHGLIAGRGGAGDAFFGEDFEIDE
                     ERPLEILSRLVGLREEHTVLLVEETVLGLLKEVIGGGRREGGMKLISTRKIREASFKY
                     SFATFSRETGAALDKLLTSIPPGVFAEPPYAPEHRETPEGQGVEAYTPLHDYELKAAG
                     RIRGECGGVCRCYELLKRHEVVELGPMELSLTGA"
     gene            7605..8948
                     /locus_tag="PLT63_04800"
     CDS             7605..8948
                     /locus_tag="PLT63_04800"
                     /inference="COORDINATES: protein motif:HMM:NF015672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98076.1"
                     /translation="MNGCADHCPEGFLSRLRDEFLVSWDDARTIVGNFHEEMRRGLAG
                     QPSSLRMLPTFAGRPRGTEQGRLLILDLGGTNVRIGEAVLDGKGRAELTSVDRSVLPR
                     EIMSASGTELFDFLADRVDHFLGGRGLDDRWCRDLAFTFSFPVEQSSLVSGRLVNWTK
                     GFTGPGVAGEDVAALLRAALARRGRERIRVAALVNDTVGTLAAGSYADPACDLGVILG
                     TGTNACYFERTDRIEKCRDGVAAGEMIVNMEWGNFNRLRTNPWDELLDRTTSNAGRQR
                     LEKMISGMYLGELARLVIGKMMAVGLLPQGTNHAALATPYALTAERLAAAVRSTDPAG
                     AVGIEGAAPVDGAAVGQICRLVAARAARLAGAAIAAVISWRDEDLETGHVVAIDGSLF
                     EKFPGFQENMREMLRELFGPRAAGIQFQLIPDGSGIGAAVVAAMGARTGWGAGSC"
     gene            8942..10150
                     /gene="amrS"
                     /locus_tag="PLT63_04805"
     CDS             8942..10150
                     /gene="amrS"
                     /locus_tag="PLT63_04805"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOD98077.1"
                     /translation="MLTRRELLANVTRVAAVAGMAAAMPPSLNGVLAASPDEELLRKA
                     PRARFWTSVESAGGDCRICHPGLTGGGGRAHRHEAHLVRCLLCAQNCTIREGQRGRCR
                     TRINIRGELRSLVYGRPVAVHVDPIEKKPFYHFLPGAAAYSLATAGCPLSCKFCQNWE
                     LSQAWPEDLRAAYTPPSLLVRSAHARRAPVIAFTYNEPTVFAEYLIDISREAKKRGLR
                     PVIVSCGFMNREPLIEMCRVLAAVKIDLKGFSASFYRQVCGAELDPVLATIKEVARQK
                     VHLEIVNLVVPTLNDSDRMLRELIAWIAGEVGLEVPVHFTRFHPDYQLLNLPPTPVAT
                     LERAREMAMAAGLRYAYVGNVPDHPGNHTYCPNCKKVVIKRTGFFLAEMQIKDGRCAF
                     CGQKIAGVWA"
     gene            10163..11692
                     /gene="amrB"
                     /locus_tag="PLT63_04810"
     CDS             10163..11692
                     /gene="amrB"
                     /locus_tag="PLT63_04810"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOD98078.1"
                     /translation="MKKRYLYIFLVFALVLVFAVVIQARKEPEMDTVRPAVVAGKFYS
                     DAPGMLKAAIAGFLEDAEPVTVSRPLALILPHAGYIYSGQIAADGYNQTRGHRYDTVV
                     ILGTNHTTPGFHRVGIYPGDGFATPLGVARVDNRIVEDLLAASPDCVRNASVHQQEHS
                     VEVQVPFIQVLFPEAKIVPVIVGSQEADLCQRFGQALGGVLKDKQALIVASSDLSHYP
                     AYAEARSVDVAILEAIPTLDAARVRQAVREAMGRGIPNLHTAACGEGPILAAMSAARV
                     MGAKGGKVISYANSGDVAIGDLSRVVGYGAVALTADAPAANAPPPPAGPAGELSPADK
                     KALLGLARKTISRYLATETVPLARGFSPAAQEYRGVFVTLKKRGQLRGCIGRLIPEAP
                     LCHLVGAMAIQSAFNDHRFRPVTATELKDIEIELSVLTPMKKVSRAEDIVVGRDGVLL
                     QKGRAGAVFLPQVAPEQGWTREEMLDQLCLKAGLPAGSWRTGAEFQTFQAVVFHESDF
                     H"
     gene            11920..13581
                     /locus_tag="PLT63_04815"
     CDS             11920..13581
                     /locus_tag="PLT63_04815"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOD98079.1"
                     /translation="MHGILPEIGISILAATALGFVFQFFRQPVILGYLVAGALIGPGI
                     GFKLVSEPANIEVISEIGLILLLFIIGLELNPRKLLSSGRQLFYAGIGQFILCVLLGV
                     GFFMLLGYGLGQGRIDALYLALFCALSSTAIVVKLLYDKFELDTLPGRITLGILIFQD
                     LWAILILALQPHFTNPRLLLVVMALSKSLVLLAIGYLLSKYLLRWIFEQIAKTPEMVV
                     AMSISWCAFMAGIGSWIGLSMEMGALIAGVAISTFPYSVHVTAKVLPLRDFFLTLFFL
                     SIGMKIPVPDAALLIMAVVIVLFVIVSRFLTIYPLLTLAGSGRRTGFIASLNLSQISE
                     FSLVVASLGVGFGHIDQRMMSLIIYAMAITSVLSSYFIKGNHAAYLLFDRFMETIGFP
                     SQAKEKEAETGAGHYPVVLLGFHRGARAFVDQLEEIAPELLKKILVIDFNLEVLKELG
                     DRGIKGIFGDISSLDTLEHAHVAEAQIILSTIPDMLLKGTNNRSMVTTCRAVASNAVI
                     VATADSVDQIDALKNCGANEVLLPYTLIGEHLARFIVTSVRPEKA"
     gene            13667..15940
                     /locus_tag="PLT63_04820"
     CDS             13667..15940
                     /locus_tag="PLT63_04820"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98080.1"
                     /translation="MKTALSPGGIAAPQPSRREGYRTHRGVVHGTMQLILFNIGLISL
                     LSQVILLRELAVASYGVELIYLYALGVWLLCTAAGALLSRRRFAVVGTIAAALFALAV
                     LLPVAVTFVRASRLLFAGVPGAYLPLARQLLIPILALGPIGLLAGFLFPPAATLYIDA
                     RPGAARTLAGAYGIESLGALAGGILATLFLKFNLSIVAATLLTSAFISLNGLALPGRK
                     RGRTAWPAGALVAAVLIALPGASRIDSMTGAWNHPHLLATGDTAYGRITVTGRSGQVA
                     VFENDVLALETEGIEGETFAHLIALQHPRPRRILLLGGGTEGIVAALRRHDPEQIDVV
                     ELNERMVRMVLPHLPPERRQALATPPVRLVFADPRGYLRAGDAYDLILVAMPDPASGQ
                     TNRFYTREFFRDCAARLRPGGIVGLRLRAAENIWPPPLTRRMAGIHWALRAEFPHVLF
                     LPGATNIITASIAPLPEDAALLSARWADRGIEARMVRPAYLEYLFTNDRFGEIRRILG
                     ATETAVNSDLQPVCYRYTLQIWLSKFFPRASFLDFAVSHRDLLMPLAVSSLLAVAGSL
                     LLVRSYPAGRRIALAAVAGFAGMILQNVLLLYYQVKNGALYQDIGLLMASFMAGLALG
                     ALLMRRRRPPAGKLRRGAVPAGFFTLAVLIHFVLFRQSGLGLPIMAGLLALSGFFSGA
                     LFAQAAQVGPDRRGGHSDPDDPERRRTLTAPLYAADLLGGAAGAVFGGLILIPAAGLA
                     LPVAITAVLIVLSLLVV"
     gene            16278..16751
                     /locus_tag="PLT63_04825"
     CDS             16278..16751
                     /locus_tag="PLT63_04825"
                     /inference="COORDINATES: protein motif:HMM:NF015237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HOD98081.1"
                     /translation="MYPRRQFRFPTMVVLGAGVIIGVVLTALMAGGYHTSGNPRFCAA
                     CHSMDHVRNRWQQSNHKQFACIECHLPAANIAEQVSYKVQAGLNDLFHETLRDYPAAI
                     LISAKGKDILRGNCLRCHQATIENTAMVKGEADCLKCHRYLVHGRGLDKGGMRVE"
     gene            16744..18027
                     /locus_tag="PLT63_04830"
     CDS             16744..18027
                     /locus_tag="PLT63_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HOD98082.1"
                     /translation="MNRKTKRRLMLLLVVAALIGVVIIFQAFVRKPESQFKLANIPAG
                     EFDPAVWGKYYPLQYESYRKNLEMSPSPTGFGGSEKVQKSVLEPEILINFKGMAFSKE
                     YTEDRGHPYAIQDLKETKRVTPASPGACMTCKTANLIDIYKSKGWQYAKTPLPELYPL
                     MKHPIVCANCHDPATMNLRVVNPAFVEAMQRRGIDVKKATREEMRSYVCGQCHVEYYF
                     EPESQKVVFPWEKGVLPEQMYAYYQERPAGFDQDWLHPDSQAKMLKAQHPEFETWQNS
                     VHGKSGVSCADCHMPYVRKHGRKYTSHWVTSPMRTTKESCMTCHTQSAEWLLQRVKTI
                     QGNAWQLQKAAGQAVARAHDVIGKAGNVAGVKQAELAKARELLRKAQWFWDIVAAENS
                     MGFHNPDQVLNTLGQSIDMAHQAAATANKAANGAF"
     gene            18089..18424
                     /locus_tag="PLT63_04835"
     CDS             18089..18424
                     /locus_tag="PLT63_04835"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOD98083.1"
                     /translation="MSETTIQNDLIARPLKLAELATYQGHSIVSREIIKKATGTVTVF
                     AFDAGEGLSEHTAPFDALVYALEGQGEIMISKTWHIVKEGEMIIMPANSPHAVKAGER
                     FKMLLVLVK"
BASE COUNT         3359 a         5731 c         5636 g         3698 t
ORIGIN      
        1 agtcggttca gtcgttcctg atgcccagga ttttccgggc ctccgtcggg gttgccgggg
       61 ggcgattaag ggaggcggcg acccgaacgg cccattccac ctgctcgaaa ctccctttcg
      121 ccatgtcccc gttgggcatg cgggtattgt cctccatccc aacccgcata tgcccgccta
      181 aaaggcagga caggacattg gcgggaaact gttccggtcc caccgcgcag gtcgtgaaat
      241 tcgcgttcgg gggcagggcg tttttcatgg cgatcatgac ctcgggacgg aatttctgtc
      301 cgcccgccac tccccaaacg aaattgaagt tcatgggctg ggagaaaaaa ccctggcggc
      361 tgatcagaag ggcgttgtcc aggccgccga tgtcgtagca ttcgatctcc ggtttgattc
      421 cgcctgtttc catggccttg ccgaaatcct ggagcatcgt gaaggtgttt tcaaagacat
      481 agtcgaaaaa gatctgcccg gaatttcggt cgatgatgct gaagttcatc gtattggtgt
      541 tcagcgaggc catctccgga cggacggcga cgatctgggc caggcgctgc tccggggtct
      601 tgaaggcccc taccgccgaa gacaggcaga tgatgacgtc cgggcatctt tccttgatgg
      661 cgtcatggac ggcccggacc cggtcgatct catgggtgga gtcgccgtca tcggtccggg
      721 cgtggacgtg cacaatggcc gctccggccc ggcggcattt ctccgcttcg tcagcgaatt
      781 cttccagggt gtagggcacc gaggggttgt tctgcttaaa ggtggcggcg ccggtaaggg
      841 cggcgcagat gatgacgggt tccgacattg gtgctgcctc ctctacatta tttatttata
      901 gacgtatttg ggacagggaa gggatcaact cgcggccctt tttatgagat acaggccgat
      961 ggcgacgccc acggccgcga acaatccgaa cagggtgaag aattgctgca cataggtgag
     1021 accgtcgtac cagctcagca cctgatagta gtagttcagg ataaaatcct ttatcgtgtc
     1081 gatcatttct gcctccgtat cgaaaggttt ggcctgctcg gttgcaattt gtgaaccttg
     1141 tttccgggag cattcaaact ctatggccca tattttatca ggaaacaaag gtgattgcca
     1201 cagtaatctt atcttcacag gggactgaaa atcgtccgac ttttggtcgt ccggccggtg
     1261 caacgtcctt gccattccct tcttgcggcg aatctcgttg caattagcgc ggggatgaca
     1321 taaggacagg cacagcagat ttctgcacgt cgatgactga tcgtttgggg aggaataaga
     1381 tgaaggtaaa atttatgggg gcggcccgaa cggtgacggg ttcctgcttc atcgtcgagg
     1441 tgggttccca tcgtttcgcc gttgactgcg gcatgcatca gggcaacaag gagatcgaaa
     1501 agcgaaactg ggataccacc ggctacgatc cggcgggtct ggagttcatc ctgatcaccc
     1561 atgcccatat cgaccacgcc ggcctcctgc cccggatcgt cagggacggc ttccggggtc
     1621 ccatttacgc caccccgccg acgctggagc tcatgaagat tctcctgctg gacagcgccc
     1681 atatccagga gatggaggcg caatggaaaa cgcgcaaatg tctccggtac ggggagacga
     1741 aggtcgaccc cctctatacc cagcgtgacg ccgaagaaac ggcgcggtac ttccgggagg
     1801 tgcgctacga tcaggccgtt tcccccggcg acgggatgac ggttgccttc cgggacgccg
     1861 gccatatcct cggcgcctcc attctggagc ttaccgtcac ggaagacggc aaggaatcac
     1921 ggctggtctt ttccggcgac gtgggccgtc cggcgcagct catcgtcaat gatcccacgg
     1981 ttatcgaaac ggcggattat ctcttcatgg aatcgaccta cggcaatcgg gatcacaagg
     2041 acgaggacga aagcctgaac gaactggcgg aagccatcga tttcagctac cggcaggggg
     2101 aaaagacgat catcccttcc ttcgccgtgg agcggaccca ggagttgctt tactgtctcc
     2161 atctcctcga cagagacggg cggctgccca aggatatgcc ggtttatgtg gacagccccc
     2221 tggccacccg ggctacggag atttttcgta aatataccaa atatttcgac gaggaatcca
     2281 aggtgctgct aaaaaaaggg caggacccgc tgggcttccc ccaactccga ttcacccgtt
     2341 ccaccgagga gtcgatgaag atcaatggcg ccgagggtcc cgccgtcgtc atttcggcca
     2401 gcggcatggc caatgccggg cgcatccgcc atcatctccg ccacaatctc tggcgtccgg
     2461 gcgccgccgt ggtgttcgtc ggctttcaag cccaggggac gacgggccgc cggatcgtgg
     2521 acggggcgaa aagtgtgcgg atattcaacg aggacgtggc ggttcaggcc cgggtattca
     2581 ccatcaacgg cttttcggcc catgccggcc agagccagct tctggagtgg ctggggggat
     2641 tcaggaatcc gggcctgcgg gtgtttctcg tccacgggga gtacaccgcc caggaagtat
     2701 tggccggcaa gatcgcggcc cgcttcggct atcgggtgga gattcccgac tacctggagc
     2761 aggtgacgct ggagccgggc aaagagatcg agcgggaggt gtatcccgag caggccaaac
     2821 cggggatcga ctgggaattt ctccttggag acctggagca gcgcctgcgc caactgcggg
     2881 aacggcggcc ccgcctggaa ggtaaaggct gggtggagca gacggacatg cgggagcggc
     2941 tgctggaatt ggaccatcgg cttgcctccc tgatctcgga gatctgacgg gacaaacccc
     3001 gctacggctt cagccggtaa tggaaagagg cgacgatatg aagccgggcg aggctcaact
     3061 ccggcgggag acggtcatag ggagcggcgg cgcgaaccac cgcgagggaa tgcttgtcga
     3121 gtacggacga tcccgaagaa gtcaggactg cctgtccgga cagaacgccc cgattctcga
     3181 tggtgaattt taccgtagcg atgccctgct cccctttttt cagcgccgcc tccgggtacg
     3241 accatcggct ttcgatccgc tgccggacct tcagcaggta ggagcgataa cggcggtccg
     3301 ggctttccag gttgacggtc tgctcctccg aggtcgtccg gttggccttt tccccggccc
     3361 ctgccggcgc ctcccggact tccggttccg gtgggagcga ttcggccagc tcgatggtca
     3421 tggatcgctc cgggtgaggc ggcggcggcg caatccggtt gcccatgacg ggcatggaga
     3481 gaaccaaggc atgggaggcg aaggaaacca gcagggcgat gaggaatgcc ttcttcatgg
     3541 ctatcttttc cagaggccgg cgggttttca tggcgggatc ataaccgtaa gaaaccgaaa
     3601 aagcaataaa gagggcgaag tataatcatg aagatcgacc atggcgcggt gcctgagcga
     3661 ggggcgaccg gcgcgcctcc cgaggacgat gcctttttca tggacctggc cctggcggag
     3721 gccgcggcgg cggccgccgc cggagaagtc ccggtggggg cggtgatcgt cctggccgga
     3781 cgggtcatcg cccgggccgg caaccgcccc atcagcctga aggaccccac cgcccacgcc
     3841 gagattctcg ccatccggga agcggcaaga ataactgata attatcgcct ccccgggacg
     3901 atcctataca ccaccctgga gccctgcccg atgtgtgccg gcgccatgat ccatgcccgg
     3961 gtgtgcaggc tcgtcttcgg gacccgggat cccaagggcg gggccgtagt ctccgccgcc
     4021 cggatgttcg acgattatcc cggcaatcac gtggtggaat atctcggggg cgtgcgagag
     4081 gagagctgcg ccgaaatttt gagtagcttt tttatgcaaa agaggttatg atcccgccgc
     4141 ttttgcaccg ggccatgagg attgcggaga gataccgaag tggtcgtaac gggatcgact
     4201 cgaaatcgat ttgggggcca aaagctccca cgggggttcg aatccccctc tctccgccaa
     4261 atatttgcgg tctcagctga tattatcccc aactgtgcgg atattttcat ctgaaagtgc
     4321 gtccctatat ggttgacttt attcactgat aatttttctc cccttcaagg ggaggactaa
     4381 ggtggggatg gttcccctat gtgacgggat accgtcatga gttttttatc tctcgtcgca
     4441 cccggtccgg gtgtggcggt tctttgaaac ggcgccgtcg gccgtgatgc atccatatgg
     4501 atggggacag gtacggcggt aggggagcgg gccgcgtgaa gccggggcga tggcctgtgg
     4561 acatatatat cgggagagat gcctgagagg ccgaaagggc acgactggaa atcgtgtgta
     4621 cgctcaaaaa agtgtaccgg gggttcgaat ccccctctct ccgccagacc ggcaggcggg
     4681 acggacggca atgccgtttg tcccgcctta tttgtttttc ggatcagcca gccggcggca
     4741 gtgcattccc ccatgccggg acgccgggat caggcgtaac ccagggcggc cagttcctct
     4801 tccgtcatac cgaggaaatg ggcgatctcg tggacgacgg tgatttcgat ttcctcctcc
     4861 agctccgcga tgctgcggca ggcatcttcc aagggttcct gaaagatgaa gatggtgtcg
     4921 gggtacaaag gcgggctgaa ttcagagcgc tcggccaggg ataccccggt ataaatgccg
     4981 aagggggcat catcgggatc cagtcccagg gtcatgctca tctgtcggga ggggcggcgc
     5041 tgcacggaga tcagaatatt gtccaggcgg tcccttatct ccgccgggat cctgccgatc
     5101 gcccgctcca ccgcccgatc gaattccctg ctgctgagtt tcatcgccat tccttcgctc
     5161 cgtcctggtt ttatacaccg tggttgccgg ggccgcaagg aagaatgatc atttcggacc
     5221 ggggcggccc gccggtggga aggacgggaa ttcccggcgt tcaaagcacc cttgacttct
     5281 ttgccgatct cctttaatag caaggaccac gataggggga tttcagcaac atgccgaata
     5341 ttttcgtatt ttgatgcgcc tacgggcagg gccgttatgc agcgggatgc aatcccgcgc
     5401 tgtcggtgaa aaagacaagg atacaatagc tatgacgtca acgagaagaa caacagccgc
     5461 ggtgaccttt tgggcggcca taatttttct ggccgcggcg gccctccccg gggagatatg
     5521 cattgccgcc ggggaggcgg gcggccacga catcggccgg gaacttccgg tctggacggc
     5581 gacccccttt gtgggcatcc tgctgtccat cgccctcttt cccctcttgg ccccccattt
     5641 ctggcaccgt cacttcggca aggtgtctgc cttctgggcc ctggccttcg ccctgccgtt
     5701 tttgtttttc ttccgggaaa cggctctcta tgagatcctc cacatcatgc tcatcgatta
     5761 catccccttc atcatcctcc tctggggcct gttcaccatc gccggcggca tcgccgtgag
     5821 cggctccctg cggggcaccc ccctgatgaa ctccctgctg ctcctcatcg gcacggtcct
     5881 ggcctcctgg gtggggacca ccggcgccgc catggtcatg atccgccccg tcctccgggc
     5941 caacaaagac cgggtgaaaa aggcccacat catctgcttc ttcatctttc tggtggccaa
     6001 tatcggcggt tccctgacgc ccctggggga cccgcccctc tttctcggct tcctccacaa
     6061 tgttcccttt ttctgggtga ccacgagcat cctgccccat atgctcctgt cgtccctcat
     6121 cctcctcgcg gtgttctatg ccttcgatct tttctattat cgccgggagg cgccggtgcc
     6181 gccggcaacc ggggaacgga cgccgatccg catcgagggt ctccacaacc tgctgcttct
     6241 cgccggggtg atcggcgtca tcctgctgag cggctactgg cggccgggac atctgtccat
     6301 tttcggcatc gaggtccagt accagaatct cctccgggac gggagcatcg tcaccctggg
     6361 cctgatttcc ctgttcgtca cgccccggcg gctccgggag gccaacgact tcagttgggg
     6421 gcccatcctg gaggtggcga aactcttcgc cgggatcttc atcaccatca ttcccgccct
     6481 ggccatcctc aaggcaggtt cccagggcgc cctggcctgg ctggtggacg cagtcaagac
     6541 cccggcccat tacttctggg cggctgggat cctctcgtcg tttctggaca acgccccgac
     6601 ctatctgacc ttcttcaaca tggccctggg gcggctcgat cttacggagg ccatgatccc
     6661 cacggccctg gccgccgggg cggcgacggc caacccccag tttatcgcct atctccaggc
     6721 gatatccgcg ggcgcggtct tcatgggggc caatacctat atcggcaacg ccccgaactt
     6781 catggtcaag tccatcgccg aggaggcggg ggtcaacatg ccgagcttct tcggctatat
     6841 gctcaagtat tccatcccga tcttgatccc gattttcatc gccgtcagtt tcgtcttttt
     6901 ctgaacagga ggcaggtcca tgagcaaaga ttactatgag gtggttgtcg agggtcatcc
     6961 ggagtacgtc aacggttttg tccacggttt gatcgccggc cggggcgggg cgggggacgc
     7021 ctttttcggg gaagacttcg agatcgatga agaacgccca ctggagatcc tgagccgcct
     7081 ggtgggtctt cgggaagaac atacggtgct ccttgtcgag gagaccgtcc tgggcttgct
     7141 gaaagaggtg atcggcggcg gccgccggga aggtgggatg aagttgatct ccacgcgaaa
     7201 gatccgggag gcgtcgttca agtattcctt cgcgaccttt tcccgggaaa ccggcgccgc
     7261 gctcgacaag cttctgacct cgatcccccc cggcgttttc gcggagccgc cctacgcccc
     7321 ggaacacaga gaaacgcccg aaggtcaggg ggtggaggcc tacacccccc tgcacgacta
     7381 cgaattgaag gcggcgggac gcatccgggg ggagtgcggg ggggtatgcc gctgctacga
     7441 actcctgaag cggcacgagg tggtcgaact cggaccgatg gaactcagcc tgacgggcgc
     7501 atagcgaaac gtttgtccgc cgccatgtaa agacggggca aacatttacc gataccgaga
     7561 gatcgtgagc ttgccatggt caagccgttg gggagacctt cctaatgaat ggatgtgcgg
     7621 atcattgccc tgagggcttc ctctcgcggt tgcgggatga attcctggtt agctgggacg
     7681 acgcccgaac gatcgtcggg aatttccatg aggagatgcg ccggggcctc gccgggcaac
     7741 ccagctcgct ccgtatgctt cccacctttg ccggccgtcc ccggggaacg gagcaggggc
     7801 gcttgctcat cctcgatctg ggcggtacga acgtccggat cggggaggcg gtcctggacg
     7861 gcaagggccg ggccgaactc acgtcggtgg acagatccgt ccttccccgg gaaatcatga
     7921 gcgcctcggg cacggaactg ttcgattttc tggccgaccg cgtggatcat ttcctcgggg
     7981 gacggggact tgacgaccgg tggtgccgcg atctcgcctt taccttttcc tttcccgtgg
     8041 agcagtcgtc cctagtttcc ggacggctgg taaactggac caaaggtttt acgggtcctg
     8101 gcgtggcggg cgaagatgtg gcggcgctgc tccgggcggc gctggcgcgc cggggccggg
     8161 aacggattcg ggtcgccgcc ctggtgaacg atacggtcgg cacactggcc gcgggaagct
     8221 acgccgatcc ggcctgcgat ctcggcgtga tcctcggcac ggggaccaac gcctgctatt
     8281 tcgagcggac ggaccggatc gaaaaatgcc gggacggcgt cgccgccggg gagatgatcg
     8341 tcaatatgga atggggaaac tttaaccggc tccggaccaa tccctgggat gaactcctgg
     8401 atcggacgac ctctaacgcc ggccggcaac gtttggagaa gatgatctcc ggcatgtatc
     8461 tgggggaact ggcccggctc gtcatcggga agatgatggc ggtgggtttg ctcccccagg
     8521 ggacgaacca tgccgccctt gcgacccctt acgccttgac cgcggagcgg ctggcggcgg
     8581 cggtccgttc cactgacccc gccggcgcgg tggggattga aggcgcggcc ccggtggacg
     8641 gagcggctgt cggacagata tgccgcctcg tcgccgcccg ggcggcccgc ctggccggag
     8701 cggcgatcgc cgccgtgatt tcctggcggg atgaggatct cgaaacgggg catgtcgtgg
     8761 cgatcgacgg gtccctgttt gaaaaatttc ccggtttcca ggaaaacatg cgggagatgt
     8821 tgcgggaatt gttcgggccc cgggcggcgg ggatacaatt ccaactcata ccggacggct
     8881 cgggcatcgg cgccgccgtc gtcgccgcca tgggcgctcg aaccggatgg ggagcgggat
     8941 catgctgacc agaagggaac tgctggcgaa cgttacccgg gtggccgcgg tggccggaat
     9001 ggcggccgcg atgccgccct ccctgaacgg cgtcctggcg gcatcccccg acgaggaact
     9061 cctccggaag gccccccgag ccagattctg gacctccgtg gagtcggcgg gcggcgactg
     9121 ccgaatctgc catcccggct tgacgggcgg cggggggcgg gcgcatcgcc acgaggccca
     9181 cctggtccgg tgcctcctgt gcgcccagaa ctgcaccatc cgggaaggcc agcggggacg
     9241 gtgccgcacc cgcatcaaca tccgggggga actccggagc ctggtatatg gtcgtcccgt
     9301 tgccgtccat gtagatccca tcgagaagaa gcctttctac cattttctcc ccggggccgc
     9361 cgcctattcc ctggccaccg ccggctgccc cctgagctgc aagttctgcc agaactggga
     9421 gctgtcccag gcctggccgg aggacctccg ggccgcctac acccctccct ccctcctcgt
     9481 ccggtccgcc cacgcccgcc gggctccggt gatcgccttc acctacaacg aaccgacggt
     9541 cttcgcggag tatctgatcg acatctcccg ggaggcgaaa aagcgcggcc tccgcccggt
     9601 gatcgtcagt tgcggcttca tgaaccggga acccctgatc gagatgtgcc gggtcctggc
     9661 ggcggtgaag atagatctca aggggttcag cgcctccttt taccggcagg tctgcggggc
     9721 ggaacttgat cccgtcctcg cgaccatcaa ggaggtcgcc cggcagaagg tgcacctgga
     9781 gattgtcaat ctcgtggtcc cgaccctgaa cgattcggat cggatgctcc gggaactcat
     9841 cgcctggatc gccggcgagg tcggtttaga ggtgccggtg cactttaccc gctttcatcc
     9901 cgattaccag ctcctgaatc tcccccccac ccccgtggcc accctggagc gggcccggga
     9961 gatggccatg gctgcggggc tccgctatgc ctatgtcggc aacgtcccgg accacccggg
    10021 caatcacacc tattgcccga actgcaaaaa agtggtaatt aagcgaacag gttttttcct
    10081 cgcggagatg caaatcaaag acgggcgttg tgccttctgc gggcaaaaga tagccggcgt
    10141 ttgggcatag aggcgacagg ccatgaaaaa gagatacctc tatatttttt tggtgtttgc
    10201 gcttgtcctg gtctttgccg tggtcattca agccagaaag gagccggaga tggataccgt
    10261 tcgccccgcc gtcgtggcgg gaaaatttta ttcggatgcg ccgggaatgc tcaaggccgc
    10321 cattgccggc tttctcgagg acgcggagcc ggtgacggtt tcgcggcctt tggccctcat
    10381 cctgccccat gcgggctaca tctattcggg acagatcgcc gccgacggtt acaaccagac
    10441 ccgaggccac cgctatgaca cggtggtgat tctggggacc aaccatacca cccccggttt
    10501 ccaccgcgtt ggcatttacc ccggcgacgg tttcgccacc cccttggggg tcgcccgagt
    10561 ggacaacagg attgtggaag acctcctggc ggcatccccg gactgcgtcc ggaatgcctc
    10621 cgtccatcag caggagcatt ccgtggaagt acaggtaccc ttcatccagg tgctgtttcc
    10681 ggaggcgaag atcgtgccgg tgatcgtcgg cagccaggag gcagatctgt gccaacgttt
    10741 cggccaggcc ctgggtggcg tcctgaaaga caagcaggcc cttatcgtgg ccagttccga
    10801 tctctctcac tatcccgcct atgcggaggc ccggagcgtg gatgtcgcca ttctggaggc
    10861 tattcccacc ctggatgcgg cgcgggtgcg gcaagccgtc cgggaggcga tgggcagggg
    10921 gatccccaat ctccacaccg ccgcctgcgg ggaaggaccc atcctcgctg ccatgagcgc
    10981 cgcccgcgtc atgggcgcca aaggcgggaa ggtgatcagc tacgccaatt ccggcgatgt
    11041 cgccatcggc gacctctccc gggtggtggg ctacggcgcc gtggccctga ccgccgatgc
    11101 cccggccgca aacgcgccgc caccgcccgc cggacccgcg ggggagttgt cgccggcgga
    11161 taaaaaggcc ctgctcggtc tggccagaaa gactatttcc cgctatctcg ccacggaaac
    11221 cgtgccgctg gccaggggat tctccccggc cgcccaggaa taccgcggcg tcttcgtcac
    11281 cctgaagaag cgcgggcagc tccggggctg catcgggcgc ctcatccccg aggccccgct
    11341 ttgccacctc gtcggggcca tggccatcca gtccgccttc aacgaccatc gcttccgccc
    11401 ggttaccgcc accgagttga aggatatcga aatcgagctg tcggtcctga caccgatgaa
    11461 aaaggtttcc cgggcggagg acatcgtggt gggccgggac ggcgtcctcc tccagaaggg
    11521 ccgggccggg gccgtcttcc tcccccaggt ggccccggag cagggttgga ctcgggagga
    11581 gatgctcgat caactttgcc tgaaggccgg tctgcccgcg ggaagctggc gcaccggtgc
    11641 ggagttccag accttccagg ccgtggtctt tcacgagtcg gatttccatt gacgggcggg
    11701 gttacggaaa acttcctggt aaaggtgtgc gaatccttgg tgatgataag tgtttgggcg
    11761 tatgttgccg tgacgaagga tgaaacgcga cgccgatatc gtcaaataat caatccctat
    11821 tcattgctgc gcaggccgcc cctttgccgt caacggcaaa agggcggtag tccaccgtct
    11881 gttgtgctca tggggtaaga aaggagatat tcatcgataa tgcatggcat tcttccggaa
    11941 atcggtatct ctatcctcgc cgccaccgcc ttggggttcg tcttccagtt ctttcgccag
    12001 ccggtcatcc tgggctacct ggtggccggg gcgctgatcg gtcccggcat cggcttcaag
    12061 ctcgtctcgg agcccgccaa catcgaggtc atttctgaaa tcggcttgat cctcctgctg
    12121 ttcatcatcg gtctggaact caatcctagg aagctcctct cttcgggccg ccagctattc
    12181 tatgccggaa tcggccagtt catcctctgc gtcctcctcg gggtggggtt tttcatgctc
    12241 ctgggatacg ggctgggtca gggccgcatc gacgccctct atctcgccct gttctgcgca
    12301 ctgagcagca cggccatcgt ggtcaaactc ctatatgaca aattcgagct ggacaccctc
    12361 cccgggcgga tcaccctggg gatactgata ttccaggacc tctgggcgat cctgattctg
    12421 gccctccagc cccatttcac caatccccgc ctcctcctgg tcgtcatggc cttgagcaag
    12481 agcctggtgc tcctggccat cggttacctc ctgagcaaat acctcctccg ctggatattc
    12541 gagcagatcg cgaagacgcc ggagatggtg gtggccatgt ccatctcctg gtgtgccttt
    12601 atggcgggga taggctcatg gatcggcctg tccatggaga tgggggcgct gatcgccgga
    12661 gtggcgattt ccaccttccc ctacagcgtc cacgtgaccg cgaaggtgct tcccctgcgc
    12721 gacttttttc ttacgctctt ctttctttcc atcggcatga aaatccccgt tcccgatgcc
    12781 gccctgctca tcatggccgt cgtgatcgtc ctgttcgtca tcgtttcccg cttcctcacc
    12841 atctacccgc tcctcaccct ggcgggcagc ggccggcgca ccggtttcat cgccagtctc
    12901 aacctctccc agatcagtga gttctccctg gtggtggcgt ccctcggggt tggcttcggc
    12961 catatcgacc agcgaatgat gtctttgatc atctacgcca tggccatcac ctcggtactc
    13021 tcctcttatt tcatcaaggg gaatcatgcc gcctatctcc ttttcgaccg ctttatggag
    13081 acgatcggtt ttccgtccca ggccaaggaa aaggaggcgg aaaccggcgc ggggcattat
    13141 cccgtggtcc tcctgggatt ccaccggggg gctcgggcct tcgtggacca gttggaggag
    13201 atcgccccgg agttgctgaa aaagatcctc gtcatcgatt tcaatctcga ggttctcaag
    13261 gaactgggag atcggggcat caaaggaatt ttcggcgaca tcagcagcct ggacaccctg
    13321 gagcacgccc acgtcgccga ggcgcaaatc atcctgtcca ccatcccgga catgctcctc
    13381 aaggggacaa acaaccgctc catggttacg acctgccggg ccgtagcatc caacgccgtc
    13441 attgtcgcca ccgccgattc cgtggaccag atcgacgccc tgaagaactg cggggccaac
    13501 gaagtgcttc tgccctacac cctgatcggg gagcatctgg cccggttcat cgtcaccagc
    13561 gtccggcctg agaaggccta accgggtaaa gagccgctgg agatagccgc cgccggccaa
    13621 cgctgtttgc ggcccgccgc cggccctatg ccggtccgcg ggggacatga aaactgcttt
    13681 gtcgccgggg ggcatcgccg cccctcagcc gagccgccgg gagggatacc ggacccaccg
    13741 gggcgtagtt catggcacca tgcaactgat cctgttcaat atcggcttga tctccctgct
    13801 gagccaggtc atcctcctcc gggagctggc cgtcgcctcc tacggcgtgg agctgatcta
    13861 tctgtatgcc ctgggcgtct ggctcctgtg caccgccgcc ggggcccttc ttagccgccg
    13921 ccgcttcgcc gtcgtcggga ccatcgcggc ggcgctgttc gcactggcgg tcctcctgcc
    13981 tgttgccgta acgttcgtcc gggcgagccg cctgcttttc gccggggtgc cgggggctta
    14041 tctccccctc gcccggcagc tcctcatccc catactggcc ctcggcccga tcgggctgct
    14101 ggccggtttc ctcttccccc ccgcggcgac gctttatatc gacgcccgac ccggcgcggc
    14161 ccggaccctg gccggggcct acggcatcga aagtctcggc gccttggcgg gggggatcct
    14221 cgccaccctg tttttgaaat tcaacctctc catcgtggcg gccaccctgt tgaccagcgc
    14281 ctttatctcc ctgaacggcc tggcactgcc gggaaggaag agagggagga cggcctggcc
    14341 ggccggggcg ttggtcgccg ccgtcctcat cgcccttccg ggagcgtccc ggatcgactc
    14401 catgaccggc gcctggaacc atccccacct gttggcgacc ggggataccg cctacgggcg
    14461 gatcaccgta accggtcgtt ccggacaggt ggcggtcttc gaaaacgacg tcctcgcctt
    14521 ggaaacggag gggatcgagg gggagacctt tgcccatctc atcgccctcc agcacccccg
    14581 gccgcgacgg atcctccttt tgggcggggg aacggagggg attgtggcgg ccctccgccg
    14641 gcacgatccg gagcaaatcg acgtggtgga actgaacgaa cggatggtaa gaatggtgtt
    14701 gccccatctg cccccggagc gccgacaggc cctggcgacc cccccggtgc gccttgtctt
    14761 tgccgacccc cgcggctacc tccgcgccgg cgacgcctat gacttgattc tggtggccat
    14821 gccggacccc gcctccgggc agaccaaccg tttttacacc cgggagttct tccgggactg
    14881 cgccgcccgg ctgcgaccgg gggggatcgt cgggctccgc ctccgggcgg cggaaaacat
    14941 ctggcccccg ccgctgaccc gccgtatggc gggcatccac tgggcattgc gggcggagtt
    15001 tccccacgtc ctcttcctcc ccggggccac gaatatcatc accgcctcca ttgctcccct
    15061 gccggaagac gcggcgctac tcagcgcccg ctgggctgac cgggggatcg aggcccgaat
    15121 ggtgcggcct gcttatctcg agtatctgtt caccaacgac cggttcgggg agatccggcg
    15181 gatcctgggg gcgacggaaa ccgccgtcaa cagcgacctc cagccggtgt gctaccgcta
    15241 caccctccag atctggctgt ccaagttttt tccccgggcg tctttcctgg atttcgctgt
    15301 ctcccatcgc gacttgctta tgcctctggc cgtttcctcc ctcctggccg tcgccgggtc
    15361 cctgctcctc gtccgatcct acccggccgg ccggcggatc gccctggcgg cggtggcggg
    15421 ttttgccggg atgatcctcc agaacgtgct gctgctgtac tatcaagtca aaaacggcgc
    15481 cctctatcag gatatcggcc tgctcatggc gtccttcatg gccggtctgg ccctgggagc
    15541 gctgctgatg cgccggaggc gtcctcccgc cgggaaactc cggcggggtg ccgttccggc
    15601 cggctttttc accctggcgg ttctgatcca tttcgtcctg ttccgacaga gcggtctcgg
    15661 cctgccgatc atggcgggcc tcctggccct ctccggcttc ttttccggcg ccctcttcgc
    15721 ccaggccgcc caagtcggtc ccgaccgtcg cggcggccac agcgaccctg acgaccccga
    15781 acgccgccgg acactcaccg cccccctcta cgccgccgat cttctggggg gcgccgccgg
    15841 cgccgtcttc ggcggcctga tcctcatccc ggccgcgggc ctcgccctcc ccgtggcgat
    15901 caccgctgtg ttgatcgtcc tgtccctgct cgtcgtctga ttgtccgacc ggcaagaaca
    15961 agcctctacg ggcgaatcgg caggatagtt ttccggagga tgggcataaa caatggggca
    16021 gttcccggac ggaaacagaa cggacacgga tccaaaccct aacgggcgtc gccatcgaga
    16081 gcggagtctc cgtctccaga acattgccgc cctcgataac ccacagccgc tgctctggcg
    16141 cggatgtttg cgcgggcatg gtgattgatt tccggcattt ttttattgac tgtataacct
    16201 cgtccatcta tagtattttc aaaagtcgag taacaacaaa tctttctcac caacagctac
    16261 gggaggctgc gggatcagtg tacccaagac gtcaatttcg ttttccgacc atggtggttc
    16321 tgggggcggg agtcattatc ggcgtggttc tcacggcatt gatggcggga gggtatcaca
    16381 cctcgggaaa cccccgtttc tgcgccgcct gccacagtat ggatcatgtc cggaatcgct
    16441 ggcagcaatc gaaccataag caattcgcct gcatcgaatg ccacctgccg gccgccaaca
    16501 tcgccgagca ggtatcctac aaggtccagg ccggcctcaa cgatctcttt cacgaaaccc
    16561 tgcgggacta tcccgcggcg atcctcatct cggcgaaggg gaaagatatc ttgcgtggca
    16621 actgtcttag gtgccatcag gcgacgattg aaaacacggc gatggtcaaa ggcgaggccg
    16681 attgcctcaa gtgtcaccgc tatctggttc acggccgcgg tctggacaaa ggagggatga
    16741 gagttgaata ggaaaacaaa gaggagactc atgttgctgt tggtggtggc cgccctgatc
    16801 ggagtcgtga ttatcttcca ggccttcgtc cgcaaacccg aatctcagtt taaactggct
    16861 aacattcccg ccggcgagtt cgacccggcg gtctggggaa agtattatcc gctgcaatat
    16921 gagagctacc ggaagaacct ggagatgtcg ccttccccca ccggtttcgg cggcagcgaa
    16981 aaggtgcaga aatccgtcct ggaacctgaa atcctgatca atttcaaggg catggccttc
    17041 agtaaagaat acacggaaga ccgtggtcat ccctatgcca ttcaggatct gaaagaaaca
    17101 aaacgggtga caccggccag ccccggggcc tgcatgacct gcaagacagc caatctgatc
    17161 gacatttata agagcaaagg atggcaatac gcgaagacac ccctcccgga gctatatccc
    17221 ctgatgaagc atcccatcgt ttgcgccaat tgccatgacc cggcgacgat gaatctccgg
    17281 gtggtcaatc cggcgtttgt cgaggccatg cagcggcgcg gcatcgatgt gaagaaggcc
    17341 acccgggaag agatgcgcag ttatgtatgc ggtcaatgtc atgtggaata ctatttcgag
    17401 ccggaatccc agaaggtcgt tttcccctgg gagaaggggg tgcttccgga gcagatgtat
    17461 gcctattatc aggagcgtcc cgcgggattt gaccaggatt ggctgcatcc cgattcacag
    17521 gccaagatgc tcaaggccca gcacccggaa ttcgaaacct ggcaaaacag cgtgcatggc
    17581 aaatccggcg tgtcctgcgc cgactgccac atgccgtacg tccgcaagca cgggcggaaa
    17641 tacacttccc actgggtgac gagccccatg cggacgacaa aggaatcctg catgacctgc
    17701 catacccaga gcgcggaatg gctcctccaa cgcgtcaaaa cgatccaagg caacgcctgg
    17761 caacttcaga aggcggcggg acaggcggtg gcccgggctc acgatgtcat cggcaaggcg
    17821 ggcaatgtgg cgggagtcaa gcaggcggag ctggccaaag cgcgggagtt gctccgaaag
    17881 gcccaatggt tctgggatat cgtcgccgcg gagaacagca tgggattcca caatcccgat
    17941 caggtcctga ataccctggg gcagtccatc gacatggccc atcaggctgc cgcaacggcc
    18001 aacaaagcgg cgaacggagc tttctgattc gcccaaaccg acggccggca actggacaat
    18061 acgtagtgaa atcaagagga ggatacccat gagcgaaaca accatccaaa atgatctgat
    18121 cgcccgaccg ttgaaactcg ccgaactggc gacctatcag gggcattcca tcgtgagccg
    18181 ggagatcatc aagaaggcga cgggaaccgt gacggttttc gccttcgatg cgggtgaagg
    18241 actgagcgaa catacagcgc ctttcgatgc cctggtttac gccctggaag gccaggggga
    18301 gatcatgatc tccaagacct ggcacatcgt caaggaaggc gagatgatca tcatgccggc
    18361 caactctccc catgccgtaa aagccggcga acgcttcaaa atgcttttgg ttctggtcaa
    18421 atag
//
LOCUS       DAOSMF010000028        18170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_3700_length_18170_cov_25.482473, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000028 DAOSMF010000000
VERSION     DAOSMF010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 18170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18170
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3700_length_18170_cov_25.482473"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2075
                     /locus_tag="PLT63_04840"
     CDS             <1..>2075
                     /locus_tag="PLT63_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HOD98084.1"
                     /translation="AGAKFSNDSYKISGGLHGVGVSVVNALSSRLDLEVRREGHVYTQ
                     SYRHGVPLAPLEMVGKTRGRGTKVTFKPNEEIFEETEFSFDILSNRLRELAFLNSGVR
                     IVLYDERTEKQNEFYYEGGIVSFVEYINRNKRVLHKNPLYVSGSREDCQVEVALQYND
                     TYIENIFSFANSINTTEGGTHLVGFRSALTRVFNNYAENNKLLKGKETLKGEDLREGL
                     ACVISVKVKNPQFEGQTKTKLGNSEVKGLVEGIVYEKIGTYLEENPPVAKQLIAKCVE
                     ASRAREAARRARELTRRKSALEVGSLPGKLADCQEKDPARSELYLVEGDSAGGSAKQG
                     RDRKFQAILPLRGKILNVEKARFDKMLQSEEIRVIITALGTGIGDGDYDISKVRYHKI
                     IIMSDADVDGLHIRTLLLTFFFRHMRELIERGYLYIAQPPLFRIADKKKEVYIHNEEK
                     MKEYILDSGVGKASVMVRNNGGTPLSGNRFLALIKKAMRAESVLDRFAKEGKDRDIVR
                     ILAGDPSFSDHDFRFPDSLARVAKRTAMAMGEEKAFSYTVEEDPDRGGMKMVFKIAKD
                     GIVTTTCIDRDLFHNLKFSELRGLLGSLSPLGEPPYEVRQEEGGLAQELPSLSELIEF
                     VMNTGKKSLSVQRYKGLGEMNPSQLWETTMDPEKRTLLQVRVDDAVVADEIFTTLMGD
                     QVEPRKDFI"
     assembly_gap    2076..2175
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2359..4869
                     /gene="gyrA"
                     /locus_tag="PLT63_04845"
     CDS             2359..4869
                     /gene="gyrA"
                     /locus_tag="PLT63_04845"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOD98085.1"
                     /translation="MDDLFQKNLPVNIEDEMKKSYMDYAMSVIIGRAIPDVRDGLKPV
                     HRRVLFAMHEMGNRWNRPYKKSARIVGDIIGKYHPHGDQAAYDTIVRMAQDFSMRYLL
                     VDGQGNFGSLDGDPPAAMRYTEIRMSRIAEEILADLEKDTVDFAPNYDESLMEPLVMP
                     AKIPTILLNGTAGIAVGVATNIPPHNLREVVDGTIALIRNPDLSIEELIGYIPGPDFP
                     TAGFINGREGILSAYRTGRGSIRIRARAMIERNARNDRESIVVTELPYQVNKANLIEK
                     ISELVKEKKIEGIADLRDESDRDGIRVVLDLKRDEVSSVILNQLYKHTQMESSFGVIM
                     LAIDNKQPRIFNLKEILQSFIDFRKQIVVRRTRFELAKATERAHILAGLIIALNNIDA
                     VVAVIKAAANPQEAAVALGARFALTDPQARAILEMRLQRLTGLEREKIDAEHTDLMKQ
                     IAYLQDILDHPHRVLEVIITELEDVRARYGDERRTEIVVSSDDIDIEDLIVEEDMVVT
                     VSHTGYVKRNPVSLYRSQRRGGRGKVGMGTKEEDFVEKIFVASTHQYILIFSSFGKVY
                     WLKVYQIPQAGRAAKGKAIVNLINLAAGERVAAILPVKEFSEERNVVMVTRQGVIKKT
                     NLAAFSNPRSTGIIAIGIDAGDELMDVQLTLGDQDIFLGTRQGMAIRFHEEDVREMGR
                     GARGVRGIMLAEGDAVVGMEIPSEGSAIATITEKGYGKRTAVSEYRLQSRGGKGTINL
                     RTAPRVGNVTGICQVTGDEDLMLISDAGKIIRFRVEEVSLIHRSTQGVKLIDLEESER
                     LVGIARVEREEETEESQENGAEEMDGGAGDGELFDTDN"
     gene            4933..5367
                     /locus_tag="PLT63_04850"
     CDS             4933..5367
                     /locus_tag="PLT63_04850"
                     /inference="COORDINATES: protein motif:HMM:NF014058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HOD98086.1"
                     /translation="MSFRFLDHTADIGVEVEGATLADLFQQGAAALLSLLTDYAAVTA
                     SIAREIHIEGADPTDLWINYLREILYLFNGEGFFVREVGEMTFAPSAAAAAGFLLTAR
                     CRGETYDPRRHLLATEVKAVTYHHAEVTETPGGWRGVFIVDV"
     gene            complement(5394..6158)
                     /locus_tag="PLT63_04855"
     CDS             complement(5394..6158)
                     /locus_tag="PLT63_04855"
                     /inference="COORDINATES: protein motif:HMM:NF005446.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOD98087.1"
                     /translation="MQKDIFSLQGRLALITGASRGIGTAIARMLADHGARLIITARKE
                     ESLQAVAAEIAARGGEVLPMACHLGEMEQIRALFDRIQKDYGKLDILVNNAGTNPFFG
                     DVLSADEKAWDKTVDVNLKGYFFMSQSAAKIMKEQGGGAIVNVASVNAVRPAPFQGIY
                     SITKAGIASMTQSFAKELAPFKIRVNAILPGLTDTKFSSLMIQSPEIMKIILPMIPMH
                     RAAQPEEMAGLVLYLVSDAASYTTGACIPVDGGMLA"
     gene            complement(6170..7237)
                     /locus_tag="PLT63_04860"
     CDS             complement(6170..7237)
                     /locus_tag="PLT63_04860"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HOD98088.1"
                     /translation="MNIIDAAQPVRAGEELDISRIESFLRKAVPGLTGGISISQFPSG
                     HSNLTYLITAGDREFVLRRPPFGRKVKTAHDMGREYRVLKALRPLYPYCPEALAYTED
                     ETVLGCPFYIMERLKGIILRKEPPPGMDLSAAAARRLCERLIDVFVELHRVDYRGSDL
                     ENLGRPEGYVQRQVTGWSERYRGARTPDAPDCEAVMAWLLERMPSASPAPAVIHNDYR
                     FDNVVLSPKDPQRIIGVLDWEMATIGDPLMDLGGALAYWIDRDDPEEAQAIRLVPTHR
                     EGMMTRREVVAHYCARMDLPEENMDFYYVFGLFRLTVIAQQIYYRFYHRQTQDRRFGM
                     LIFAVHVLEKKARQVMTTGKI"
     gene            complement(7234..8427)
                     /locus_tag="PLT63_04865"
     CDS             complement(7234..8427)
                     /locus_tag="PLT63_04865"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOD98089.1"
                     /translation="MDFLVSEKMQAVIAMMTEFVDKELIPLEPEFLRKDFRELLPELA
                     EKRRMVKQMELWAPNHPRDCGGMGLDLMEHALVSEVLGRSPLGHYVFGCQAPDAGNVE
                     ILHKYGTEAQKDKYLLPLVAGEIRSCFSMTEVGLPGSNPVMMDATAVKEGGDYVINGQ
                     KWYSTAADGAAFAIVMAVTNPGEAPHLRASMIIVPTDTPGFNLVRNISVMGHTGSDYF
                     SHAEILYQSCRVPQTNLLGPEGWGFVIAQERLGPGRIHHCMRWIGICQRALELMCRRA
                     ARRVITMDGKTLATRQIIQAWIAESAAEIQAARLMTLHAAWKIETLGAKAARDDISLI
                     KFFVADVLQKVVDRALQVHGGLGMTDDTILAFFYRHERAARIYDGADEVHKMSVAKRI
                     LKRNA"
     gene            complement(8561..9259)
                     /locus_tag="PLT63_04870"
     CDS             complement(8561..9259)
                     /locus_tag="PLT63_04870"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOD98090.1"
                     /translation="MSSLFLIRHGQASFGTGDYDRLSPTGIAQAELLAKYWLQTGQEF
                     DAVYAGEMKRQIDTAATVLARFREVGRNAPELRILPEFNEYDSKGILTALLRKQEAED
                     PDISRFYTDKKSFQRVFEKAIRRWIAGEERIAGVTSWEDFRSRVARGITRVRQENGRG
                     KAVLVFTSGGPISAAFQIATGIPDLGTLHIVWHIVNASISTFAYDADRFSLTSFNVRA
                     HLEMAGDPALITYR"
     gene            complement(9256..11583)
                     /locus_tag="PLT63_04875"
     CDS             complement(9256..11583)
                     /locus_tag="PLT63_04875"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOD98091.1"
                     /translation="MTCDKHMPVMPGVMTRNAGKPRQAGAPADSDLKEKKSAMELAEE
                     IIGKTKAAGRKTLTEAESKQVLKACGIPVVAEETAGSLDEALAAAARIGYPVVLKGLG
                     TRLTHKTERGLVHLNLRDREEARTAAEKIMADAGADLEGFLVQPMITGRREFVAGLFC
                     DPQFGPVVMFGLGGIFTEALGDVVFRVAPVSEAQAEAMLDELRSRNLLGPFRGEAAAD
                     RRELVATITGLSRLGLTAPDITEIDINPLLIDPQGTVCAVDALIVLGERPVANEPRPP
                     VKVEDIARIFHPRSVAFIGASAQLGKWGHLLMTDLVTGGYEGGIYLVNAKGQDIAGRK
                     TYPSVADIPDHVDLGIVTLPASQVRDLIPKFQAKGIKYMLLISSGFSEVGAEGREMER
                     ELVREAAAAGVVILGPNTMGILNPHRKLYCTGTPVWPKAGSIGLVAQSGNLGTQLLAF
                     AEREGIGIRAFSGSGNEAMITIEDYLNGFEVDELTKTVVLYIESIKNGRRFFTAAWRV
                     AKKKPVIVLKGGRTAAGNRAAASHTGAMAANIKLFNAACRQTGVVVADQPMDLLDLSA
                     AFSSLPLPKGKRVAMLTMGGGWGVVASDLCIESGLEAPHLTPDVIAEIDKLLPPYWNH
                     SNPVDLVGQFDPAIPLQVMETLVKWDQCDAVIHLGAVGRNYFVRNMVKATTTADPTYP
                     EAFVNLGVEREARGEDAFYKKSAELMEKYGKPILGVILLEDENTKTVTDVPGSPYKGV
                     TFLTPERAVKALARMAEYQVWLAREERQNKARQLA"
     gene            11816..12124
                     /locus_tag="PLT63_04880"
     CDS             11816..12124
                     /locus_tag="PLT63_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98092.1"
                     /translation="MFDALRKAVLFGAGLAVMTTEKMKDFAEELVKKGEMSEKEAREA
                     LKEWMEKSKQARKDMEEKTEAMVNKVMNRMNIPTRSELEELKERLAKLEQAKKAKESA
                     "
     gene            12255..13949
                     /locus_tag="PLT63_04885"
     CDS             12255..13949
                     /locus_tag="PLT63_04885"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HOD98093.1"
                     /translation="MRLRRLGSIARTYRNINRYRQILTVLFKYGFDGIIDRLNLGRYI
                     EMGVRLLSRRQQKEVEALNNYERIRLVCEELGPTFVKMGQVLSTRPDLIPIEMVEELA
                     KLQDDVSPFPFPQAKIIIEEELQAPLEEVFAAFEEVPIAAASIAQVHRARLITGEDVA
                     VKIQRPGIPKTIGEDLGILFQLAGLLEKYIEELRLYRPTRIVEEFARTIRKEINFNLE
                     ASHAERFARQFRGNPAVYVPRVFRHASTERLLTMEYIEGIKINDIALLEAQGCDRALL
                     AARGTELILEQVFIHGFFHADPHPANMFVLPGNVICTLDFGMMGHLDARDREHFTEVI
                     MGYVSRDVPKITDAVLKIVEFQQEPDRRLLERDIATFADLHLYKPLKDVRVGEILEDL
                     IELISRHRLRLPPDIFFMIKAMTQVEGIGLQLDPDFDITVKVRPFIKRLITERLQPKQ
                     LLLKAFDSTAALLGHLRDLPEDLLSTLRQVREGKIQIGFEHRGLERLIFELNRSSNRL
                     AFALIIAAIVVGSSLLLAAEIGPAVFGLSIIGLLGFSLAGLLGLALLIFIIRSGIL"
     gene            13958..14737
                     /locus_tag="PLT63_04890"
     CDS             13958..14737
                     /locus_tag="PLT63_04890"
                     /inference="COORDINATES: protein motif:HMM:NF013328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HOD98094.1"
                     /translation="MPELPEVETLIRQLRNVITTERIRAVRIYDEKLKYLPEVIGRRV
                     RAVRRAGKGVEIDLGGPVIHIHLRMSGRLYWRPAGDIIPSHARFSIFFDTGEILCIDP
                     RRFATLTLYCGPAALRNRSVNLLAGMNVPEICARAGDRRLPVKAFLLDQQVMPGMGNI
                     YACEILFAAAVSPWRRTGDVTAVEWGRIAAAAGSILKEAVFCRGTSISDWRDLFGRPG
                     EYQRRLKVYDREGKICFRCPARIERRKLSGRGTYYCPGCQK"
     gene            complement(14743..15921)
                     /locus_tag="PLT63_04895"
     CDS             complement(14743..15921)
                     /locus_tag="PLT63_04895"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOD98095.1"
                     /translation="MEKGSGQGKTSAAPTLAVIGAGPGGLHAAIAAARRGMKVVLFEK
                     GDVGDRIVCGECIFDSLGMLPPPGAGRLYDVRTILFSARGTYRLPIKNYRHLWMVDRR
                     TWQRQLAAQAVALRVDVRCGEKITPAGVGELVSRYDWVIDASGAPSVTSRAYGFNGEY
                     RRRPLLAYQYTVAGDFSALFSAATIKVGFLSWAPAECLPGYYWIFPKSAGVANVGVVF
                     TRGGEDGFPLDLKGLITKIMGRESLRPERVLRKGGGLIPTHMLPRLVYGRIVLVGDAA
                     GLTSPLHGGGIDLARISGIRAVEALSKGKQGVGAYRRELLSLIKHKLVLEEMLIAKMK
                     TLSFTDFDDLLHAAAVRRAGIRTKVGLRNPGLLAAAWCWLKARRLPTVISDEAVKNRD
                     "
     gene            complement(16039..16515)
                     /locus_tag="PLT63_04900"
     CDS             complement(16039..16515)
                     /locus_tag="PLT63_04900"
                     /inference="COORDINATES: protein motif:HMM:NF015827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HOD98096.1"
                     /translation="MNLVLKLWSTRDRSPVVSWTGTALLVVAALLCGCQSPGAEVQRN
                     HDVKAAMANPASVNCLEKGGRLIIQKRGDGGEYGICVFEDDRRCEEWALFRGECPPGG
                     KKITGLVTPAAQYCVITGGSYIVTGAANTPTEQGSCLLKDGRSCDVWDHYEGKCPR"
     gene            complement(16524..17246)
                     /locus_tag="PLT63_04905"
     CDS             complement(16524..17246)
                     /locus_tag="PLT63_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98097.1"
                     /translation="MSEKPLGACVVLSMSLHGYPNPEWVLHHFPISLDEYHRQRTIKF
                     KGDTTKDAQGAEKRLTPVRLCFYMTLQLTNFTWFQEMTRHEKTRYLSVLIPLAMLLSA
                     CFGQAHHKLPAATVDVDAKSDWIDTGIRVVKGQTVFFRSEGAWAVAPHDERERWPDAG
                     PEGHGNHPGEVVHRAGDHKKELPGTPFGTLLGRVSRHVFPIGIRNEIIMPASGTLYLV
                     INDYPFYRHDNRGSLKVTISKE"
BASE COUNT         3708 a         5406 c         5256 g         3700 t
ORIGIN      
        1 gccggggcga aattttccaa cgacagctac aagatctccg gcggcctcca cggcgtgggc
       61 gtctcggtgg tcaatgccct ttcctcgcgt ctcgatcttg aggtgcgccg ggaaggtcat
      121 gtctataccc aatcctaccg ccacggcgtc ccgctggccc ccctggagat ggtcggcaag
      181 acccggggca ggggcaccaa ggttaccttc aagcccaacg aggagatctt cgaagaaacg
      241 gaattcagct tcgatatcct gtccaatcgc ctccgggagc tggcctttct caattccgga
      301 gtccggatcg tcctgtacga cgagcgtacg gaaaagcaga acgagtttta ctacgaggga
      361 gggatcgtct ctttcgtaga gtatataaac cgcaataaac gggtgctcca caaaaatccc
      421 ctttatgtga gcggttcccg ggaagattgc caggtcgagg tggccctcca atacaacgac
      481 acctatattg aaaatatctt ttcctttgcc aacagcatca ataccacgga gggcggaacc
      541 cacctggtgg gatttcgttc cgccttgacc agagtgttca acaactatgc ggaaaacaac
      601 aagttgctga agggaaagga gaccctcaag ggggaggacc tccgggaggg actggcctgc
      661 gtcatcagtg tcaaggtcaa gaatccccag ttcgagggtc agaccaagac aaagctcggg
      721 aacagcgagg tcaagggttt ggtcgagggg atcgtctatg aaaagatcgg gacctacctg
      781 gaagaaaatc ccccggtggc caaacagctc atcgctaaat gcgtcgaggc ctcccgggcc
      841 cgggaggcgg cccgccgggc ccgggaattg acccgacgga agagcgcctt ggaggtggga
      901 tcactgccgg gtaaactggc ggactgccag gagaaggacc cggcgcgcag tgaactgtat
      961 ctggttgaag gggattccgc cggcggttcg gccaagcagg gaagagacag gaaattccag
     1021 gcaatcctcc cgctccgcgg caagatcctc aacgtcgaga aggcccgttt cgacaagatg
     1081 ctccagagcg aggagatccg ggtcatcatc acggccctgg ggacggggat cggtgatgga
     1141 gattatgaca tcagcaaggt acgttatcat aagatcatca tcatgtccga tgcggatgtg
     1201 gacggtcttc acatccgcac actccttttg accttctttt tccgccacat gcgggagctt
     1261 atcgaacggg gctatctgta catcgcccaa ccgccgctct tccggatcgc cgacaagaag
     1321 aaggaggtct atatccataa cgaggagaag atgaaggagt atatcctcga cagcggcgtc
     1381 ggcaaggcga gcgtcatggt ccgcaacaac ggcggcaccc ccctgtccgg aaaccgcttt
     1441 ctcgccctga tcaaaaaggc catgcgggcg gaaagtgtcc tcgacaggtt cgctaaggag
     1501 ggtaaggacc gggatatcgt taggattctc gccggtgatc cgtccttctc cgatcatgac
     1561 ttccgtttcc ccgactcact ggcgcgggtg gcgaaacgca ccgctatggc catgggggaa
     1621 gagaaggcct tcagctacac agtggaggag gatccggatc ggggggggat gaagatggtc
     1681 ttcaaaatcg ccaaagacgg catcgtcacg accacctgca tcgaccgcga cctgtttcac
     1741 aaccttaaat tttcggaact gcgcggcctc ctcggtagtc ttagccccct cggcgagccg
     1801 ccctatgagg tccgtcagga agagggcggt ttggcgcagg aactgccgtc attatcggaa
     1861 ctcatcgaat tcgtcatgaa caccggaaaa aaatccctct ccgtccagcg atacaagggt
     1921 ctgggggaga tgaatccctc ccagctttgg gagacaacca tggatccaga gaaacggacc
     1981 ctcctgcagg tccgcgttga cgacgccgta gtggccgatg aaatcttcac cacccttatg
     2041 ggagaccagg tcgaaccccg caaggatttc atttannnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnaaatc ttcaccaccc ttatgggaga ccaggtcgaa ccccgcaagg
     2221 atttcattta caaaaacgcc ctttacgcca ccaatctgga tctgtaaaag gggacggtta
     2281 tctttttgac ccggccgcgg aaaggcggac aaaaccgttg ccgtttacat cactttgttc
     2341 aggagctgca tataaattat ggatgatctc tttcagaaaa acctgccggt caatatcgaa
     2401 gacgaaatga agaagtccta catggactac gccatgagcg tcatcatcgg ccgggccatt
     2461 cccgacgtcc gggacggcct gaaacccgtt caccgccggg tcctctttgc catgcacgag
     2521 atggggaacc gatggaaccg tccctacaag aaatcggcgc gtatcgtcgg cgacatcatc
     2581 ggcaagtacc acccccacgg cgatcaggca gcctacgaca ccatcgtccg catggcccag
     2641 gacttcagca tgcgctatct cctcgtggac ggccagggca actttggctc cctggacggc
     2701 gacccaccgg cggcgatgcg ttacaccgag atccgtatgt caaggatcgc cgaagagatt
     2761 ctggcggatc tcgaaaagga cacggtagat ttcgctccca attacgacga atcccttatg
     2821 gaaccgctgg tcatgccggc caagatcccc acgattcttc tcaacggcac cgccggcatc
     2881 gccgtggggg tggcgacgaa catccccccg cacaatctcc gggaggtggt cgatgggaca
     2941 atcgccctga tccgtaatcc cgatctgagc attgaggaac tcatcggcta catccccggg
     3001 ccggacttcc cgacggcggg attcatcaac ggccgggagg gcatcctctc cgcttaccgt
     3061 accggccggg ggagcatccg gatccgcgcc cgggccatga tcgagcgcaa cgcccggaac
     3121 gaccgagaga gcatcgtcgt tacggaactt ccctaccagg taaataaggc caatcttatc
     3181 gagaagatat cggaactcgt caaggagaag aagatcgagg gcatcgccga tctacgggac
     3241 gaatcggatc gggacggcat ccgggtggtc ctggatctga aacgggacga ggtttccagc
     3301 gtgatcctta accagctcta caaacacacc cagatggaat cgtccttcgg tgtcatcatg
     3361 ctggccatcg acaacaagca gccccggatt ttcaatctca aggagatcct ccagagcttc
     3421 atagatttcc gcaagcagat cgtcgtccgc cggacccgct tcgagctggc caaggccacg
     3481 gaacgggccc acatcctggc ggggctcatc atcgccctga acaatatcga cgccgtggtc
     3541 gcagttatca aggccgccgc gaatccccag gaggccgccg tggccctggg cgcccgcttc
     3601 gccctcacgg atccccaggc ccgagcgatc ctggagatgc gcctccagcg tctcaccggt
     3661 ctggagaggg aaaaaatcga cgccgaacat acggatttaa tgaaacagat cgcctacttg
     3721 caggacattc tcgatcaccc ccaccgggtc ctggaggtaa tcatcaccga gttggaagac
     3781 gtccgggccc gatacggcga cgagcgacgt acggagatcg tggtcagctc cgacgacatc
     3841 gacatcgaag accttatcgt cgaggaggac atggtcgtaa cggtctccca caccggctat
     3901 gttaagcgca atcccgtgag cctctaccgg agccagcgcc ggggcggccg cggcaaggtg
     3961 ggcatgggca ccaaggaaga ggattttgtg gagaagatct tcgttgcctc tacccaccag
     4021 tatatcctta tattttccag cttcgggaag gtttactggc tgaaggtcta ccaaatccct
     4081 caagcggggc gcgcagcgaa gggcaaggcc atcgtcaacc tcatcaacct cgccgcgggg
     4141 gaacgggtgg cggccatcct ccccgtgaag gaattttccg aggaacgcaa cgtcgtcatg
     4201 gtcacccggc agggggtgat caaaaagacc aatctcgcgg ccttctccaa tccccggagc
     4261 accgggatca tcgccatcgg tatcgacgcg ggcgacgaac tcatggacgt ccagttgacc
     4321 ctgggcgacc aggacatctt ccttgggacc cgtcagggta tggcgattcg cttccatgag
     4381 gaggatgtcc gggagatggg ccggggcgcc cggggcgtcc gaggaatcat gctagccgaa
     4441 ggagacgctg tggtgggcat ggagatcccc tccgagggga gcgccatcgc caccattacg
     4501 gaaaagggtt atggcaaaag gacggcggta agtgaatacc gtctccagag ccggggcggc
     4561 aagggaacca tcaacctccg cacggctccc cgggtgggca acgtcaccgg tatctgccag
     4621 gtaacgggtg atgaggacct gatgctcatc agcgacgcgg ggaagatcat ccgtttccgg
     4681 gtcgaggagg tgtccctgat ccaccggagc acccagggtg tgaagctcat tgatctcgaa
     4741 gagagcgagc gcctcgtggg cattgcccgg gtggagcggg aggaggagac cgaggaaagc
     4801 caagaaaacg gagccgagga gatggatggc ggcgccgggg acggagaact cttcgatacg
     4861 gataattaat tagaacaaag gtgatctccc ggcatgttat tccaccccgg tgcggggagg
     4921 cagaaggagg ggatgagttt ccggttcctt gaccataccg ccgacatcgg cgtcgaggtg
     4981 gagggagcga ccctggccga tcttttccag cagggggcgg cggccctcct ctccctcctt
     5041 acggactacg ccgccgtaac cgcgtccatc gcccgggaga tccacatcga aggcgcggac
     5101 ccgacggacc tctggatcaa ttatctgcgc gaaatcctct atcttttcaa cggggaaggc
     5161 ttttttgtcc gggaggtggg ggaaatgacc ttcgcccctt ccgccgccgc cgctgccggc
     5221 tttttgctca ccgcccgctg tcgcggcgaa acttacgatc cccggcgcca tctcctcgca
     5281 acggaggtca aggccgttac ctaccaccat gccgaggtca cggaaacgcc gggcggttgg
     5341 cggggggtct tcatcgtcga tgtgtgatgt aaaccccaca ttcccccctg tcatcaggcc
     5401 agcatcccgc cgtccacggg gatgcacgcc ccggtcgtgt atgacgccgc atcggaaacc
     5461 agatagagga ccaggccggc catctcctcc ggctgggcgg cccggtgcat ggggatcatc
     5521 gggaggatga tcttcatgat ctcgggactt tggatcatga gggaggagaa cttcgtgtcc
     5581 gtcagtccgg gaagaatggc gttgacccgg atcttgaagg gcgccagctc cttggcaaaa
     5641 gactgggtca tggaggcaat tcccgccttg gtgatggaat agatgccctg gaaaggggcg
     5701 ggacggacgg cgttgacgga ggcgacgttc acgatggcgc cgccgccctg ctccttcatg
     5761 attttcgcgg ccgactggct catgaagaaa tatcctttca gatttacatc caccgtcttg
     5821 tcccaggcct tctcatcggc gctcaggaca tccccgaaga agggattggt gccggcattg
     5881 tttaccagga tgtccagttt gccgtaatct ttttggatcc ggtcgaaaag agcgcggatc
     5941 tgctccattt cgcccagatg acaggccatg ggcaggacct ctccgccccg ggcggcgatc
     6001 tcggcggcca cggcctgcaa gctctcctcc tttctggccg taatgatcag ccgggcgccg
     6061 tggtcggcga gcatccgggc gatggccgtg ccgatgccgc ggctggctcc cgtaatcagg
     6121 gcgagacgtc cctgcaaaga aaagatgtct ttttgcattt tctgtcctcc tatatttttc
     6181 ctgtggtcat aacctgccgc gccttctttt ccaggacgtg gacggcgaag atcagcatgc
     6241 cgaagcgccg gtcctgtgtc tggcggtggt agaatcggta atagatctgc tgggcgatga
     6301 cggtgaggcg aaagagtccg aagacataat aaaaatccat attttcctcc gggaggtcca
     6361 tccgggcgca gtaatgcgcc acgacctccc ggcgggtcat catcccctcc cggtgggtgg
     6421 ggaccagacg gatcgcctgc gcctcctcgg gatcatctcg gtcgatccag taggccagcg
     6481 ccccgcccag atccataagg ggatcgccga tggttgccat ttcccagtcc aggacgccga
     6541 taatccgctg ggggtctttc ggggacagga cgacattgtc gaagcggtaa tcgttgtgga
     6601 ttaccgcggg cgccggggag gcggacggca tccgttccag gagccaggcc attacggcct
     6661 cgcagtccgg ggcatcgggg gtccgggcgc cccggtagcg ctccgaccag cccgttacct
     6721 gacgctggac gtatccttcc ggccggccca ggttttccag atcgctgccc cggtagtcca
     6781 cccggtgcag ttccacgaag acgtcaatca ggcgttcgca gagccggcgg gctgccgcgg
     6841 cggagaggtc cattcccggg ggcggttcct tgcggagaat gatgcccttg aggcgctcca
     6901 tgatgtagaa cggacagccc agcaccgtct cgtcctcggt gtaggctaga gcttccggac
     6961 aatagggata aagggggcgc agggccttca ggacccggta ctcccggccc atgtcgtggg
     7021 cggtcttgac cttgcggccg aacggcggcc gccgtaggac gaactcccgg tcgccggcgg
     7081 tgatgagata ggtcagattg gaatggccgc tgggaaactg ggagatggag atgcctcccg
     7141 tcaatccggg aacggccttt cggagaaaag actcgatcct gctgatgtcc agctcctcgc
     7201 cggcgcggac cggttgcgct gcgtcgatga tattcatgcg ttcctcttta aaattctttt
     7261 ggcgaccgac atcttgtgga cttcatcggc cccgtcgtaa atacgcgccg cccgctcgtg
     7321 acggtagaaa aaggcgagga tggtgtcgtc ggtcataccc aggccgccat ggacctggag
     7381 ggcccggtcg accaccttct ggaggacgtc cgccacgaaa aacttgatga gggagatgtc
     7441 gtcccgggcc gccttggccc ccaatgtttc gatcttccag gccgcatgaa gggtcataag
     7501 tctggccgcc tggatctccg ccgccgactc ggcgatccag gcctggatga tctggcgcgt
     7561 cgccagggtc ttgccgtcca tggtgatgac ccgccgggcg gcgcgacggc acatgagctc
     7621 cagggcccgc tggcagatgc cgatccagcg catgcagtga tggatccgcc ccggtcccaa
     7681 acgctcctgg gcgatgacga agccccagcc ttccggaccg aggaggttgg tctggggaac
     7741 ccggcaggac tggtagagga tctccgcgtg gctgaaatag tcgctgcccg tgtggcccat
     7801 gacggagatg ttgcggacca gattgaaccc cggggtatcg gtggggacga tgatcatgct
     7861 cgcccggagg tgcggcgcct ccccgggatt ggtcaccgcc atgacgatgg cgaaggcggc
     7921 cccgtcggcg gcggtgctgt accatttctg tccgttgatc acataatccc cgccctcctt
     7981 aacggcggtg gcatccatca tgacgggatt cgaccccggc agacccacct cggtcatgga
     8041 gaagcaactc ctgatctccc ccgccaccag gggcagaaga tacttgtctt tctgggcctc
     8101 tgttccgtat ttgtgaagga tttcgacgtt gcccgcgtcc ggggcctgac agccgaagac
     8161 gtagtgtccc aaggggctgc gtcccagtac ttccgagacg agggcatgct ccatgagatc
     8221 caggcccatg ccgccgcagt ccctgggatg attgggggcc cacagttcca tctgcttcac
     8281 catccggcgc ttttccgcca gctccggcaa cagttcgcga aagtccttac gcaaaaactc
     8341 cggctccagg gggatcagtt ccttatccac aaattccgtc atcatggcga tgacggcctg
     8401 catcttctcc gataccagaa aatccattcg tactcctttt tttcaccgca gccatggtaa
     8461 aagcctcggt gcgatgtgaa caaaagtttt tatcttcttg gcggcgggca tatggttgcg
     8521 aggggccgcc tctccgcggc ggcggcttgt aacgatcccg tcagcggtag gtaatcagag
     8581 cgggatcccc ggccatttcc agatgtgccc ggacgttgaa ggaggtcagg ctgaaacggt
     8641 cggcatcgta ggcaaaggtg gaaatggagg cattgacgat gtgccagacg atgtgcagcg
     8701 tcccgagatc gggaatgccc gtggctatct gaaaggccgc cgagatgggt ccgccggagg
     8761 tgaagaccag gacggcctta ccccggccgt tttcctgtct tacccgtgtt atgccccggg
     8821 ccacccgcga ccggaaatcc tcccaggagg tcaccccggc aatacgctct tccccggcga
     8881 tccagcgccg gatcgccttt tcaaacaccc tctggaagga ctttttgtcg gtataaaagc
     8941 gagagatatc cggatcttct gcctcctgct tccggagcag ggccgtgagg atgcctttgg
     9001 agtcgtattc gttgaattcc ggaagaatcc gcagttccgg cgcgtttcgc cccacttccc
     9061 ggaagcgggc cagcactgtg gccgccgtgt cgatctgccg tttcatctca ccggcataga
     9121 cggcgtcgaa ttcctgtccg gtttgcagcc agtattttgc caggagttcc gcctgggcga
     9181 tccccgtcgg ggagaggcga tcgtaatcgc ccgttccgaa gctggcctgg ccgtggcgga
     9241 tcaggaaaag ggaactcatg ccaactgccg cgccttgttc tgccgctcct cccgtgccag
     9301 ccagacctgg tattccgcca tcctggctag ggctttgacg gcccgctccg gggtcaggaa
     9361 ggtcacccct ttgtaggggc tccccggcac gtccgtgacc gtcttggtat tttcgtcttc
     9421 gagaagaata acccccagga tcggtttccc gtatttttcc atcagctcgg cgctcttttt
     9481 ataaaaggca tcttctcccc gggcttccct ttccaccccc aggttgacga atgcttccgg
     9541 ataggtggga tcggccgtcg tggtggcctt aaccatgttg cggacgaagt agttccggcc
     9601 caccgctccc agatggataa cggcatcgca ttgatcccac ttgacgaggg tttccataac
     9661 ctgcaggggg atcgccggat cgaactgacc taccagatcc accggattgg aatggttcca
     9721 gtaggggggc aggagcttgt cgatttccgc gatgacgtcg ggggtaagat ggggcgcctc
     9781 cagtcccgat tcgatgcaga ggtcggaggc caccaccccc cagccaccgc ccatggtgag
     9841 catggccacc cgcttgcctt tcggtagcgg gagggaagaa aacgccgcgg acagatcgag
     9901 gagatccatg ggctgatccg cgaccaccac accggtctgg cggcaggcgg cgttgaagag
     9961 cttgatatta gccgccatgg cgccggtgtg gctggcggcg gcgcggttgc ccgccgccgt
    10021 gcggccgccc ttcaggacga taaccggttt tttcttcgca acccgccatg cggcggtgaa
    10081 gaagcggcga ccgttcttga tgctttctat atataggacc acggtcttgg tcaactcatc
    10141 cacctcgaaa ccgttcaggt agtcttcgat ggtaatcatc gcctcgttgc ccgaaccgga
    10201 gaaggcccgg atgccgatcc cttcccgttc ggcgaaggcc agcaactggg tgcccagatt
    10261 tcccgactgg gccacgagtc cgatggagcc ggccttgggc cacaccgggg tgccggtgca
    10321 gtagagtttc cggtggggat tgaggattcc catggtgttg ggcccaagaa tcaccacgcc
    10381 ggccgcggcc gcttcccgga ccaattccct ttccatttcc cggccttccg cacctacctc
    10441 actgaatccg gaggatataa ggagcatgta tttgattccc ttggcctgaa atttcggaat
    10501 caggtcccgg acctgggatg ccggcagcgt gacaatgccg agatcgacgt ggtcggggat
    10561 gtcggccacg gaaggatagg tcttgcggcc ggcgatgtcc tggcccttgg cgttgaccag
    10621 atagattcct ccctcgtatc ctccggtgac caggtcagtc atcagcaaat gaccccattt
    10681 gccgagttgg gcggaggcgc cgatgaaggc cacggaccga ggatggaaga tgcgggcgat
    10741 atcctccacc tttaccggcg gacgcggctc attggccacc gggcgttctc ccaggacaat
    10801 cagggcatcc acggcgcaca ccgtgccctg ggggtcgatc agcaggggat tgatgtctat
    10861 ttcggtgatg tccggggcgg tcagacccag ccgggacaat ccggtaatgg tcgcaacgag
    10921 ctcgcggcgg tcggccgccg cctcgccgcg aaagggaccc aggagattcc gggaccgcaa
    10981 ttcatccagc atggcctcgg cctgggcctc gctaaccggc gccacccgga aaacaacgtc
    11041 acccagggcc tcggtgaaga tgccccccaa gccgaacatg accaccggac cgaactgggg
    11101 gtcgcagaaa agaccggcca cgaattcccg gcgacccgta atcatgggct ggaccaggaa
    11161 accctccaga tccgcaccgg cgtcggccat gatcttttcc gccgccgtcc gcgcctcctc
    11221 ccggtcccgg agattcaggt gcaccagacc cctttccgtc ttgtgggtca gccgggtacc
    11281 cagtcccttg agcaccacgg ggtaaccaat ccttgccgcc gccgctaggg cttcgtcgag
    11341 ggaccccgcc gtctcctccg ccacgacggg aatgccgcag gccttcagga cctgtttcga
    11401 ttccgcctcc gtgagagttt ttcttcctgc cgccttcgtt ttgcctatga tctcttctgc
    11461 caattccatc gctgattttt tctccttaag atcgctatcc gccggcgctc cggcttgccg
    11521 cggcttgccg gcattacggg tcatgactcc cggcatgacg ggcatgtgct tatcacacgt
    11581 catgactaac tgcaaacatc atctaaatat tcttcatatt tattgacttt ttccattgat
    11641 gaggctacgg ttttcgaacc ttcgcatatc ggcggcggcc atatcggggc gattgcaaaa
    11701 gtgagaaaac atccgtataa gaaggctcga aaaatggcgg taagggtaaa aggtcattag
    11761 cagcattaag ctgtgttaac gcatgataac ttaaggaggg aaaggagaaa gggttatgtt
    11821 tgacgcattg agaaaagcgg ttctctttgg cgccggcctt gccgtaatga ccacggagaa
    11881 gatgaaagac tttgcggaag aactggtaaa gaagggggaa atgtcggaga aagaggcacg
    11941 ggaagccctc aaggaatgga tggaaaagtc gaaacaggcc cgcaaggata tggaagagaa
    12001 gacggaggcg atggtcaaca aggtgatgaa ccggatgaac atacctaccc gttccgaact
    12061 ggaagagctt aaggagcgct tggccaaact ggaacaggca aaaaaagcca aggaatctgc
    12121 ctgacctccc ggcagttatg atgcattatc gaaaatcttg cccgctccat tccgaaatgg
    12181 atggagagga tatcctttta ccggccgctc gatcaccatt aggtggttaa aaacagcata
    12241 tataacgata atttatgcga cttcgccgct tgggcagcat tgcccgcacc taccgcaaca
    12301 tcaaccgcta ccggcagatc ctgacggtat tgttcaaata cggttttgac gggatcatcg
    12361 accggttgaa tctcggccgt tacatcgaga tgggggtgcg cctcctgtcc cggaggcagc
    12421 agaaagaagt cgaagccctg aacaattacg aacggatccg cctggtctgc gaagagctgg
    12481 ggccgacctt cgtgaagatg ggacaggttc tttccacccg ccccgacttg atccccatag
    12541 aaatggtgga ggagctggcc aaactccagg acgatgtttc tccctttccc tttccccagg
    12601 cgaagatcat catcgaagag gagctccagg cccctctgga ggaggtattt gccgccttcg
    12661 aggaggttcc gatcgccgct gcctccattg cccaggtgca ccgggcccga ttgatcaccg
    12721 gcgaggacgt ggcggtcaag atccagcgtc cggggatacc caagaccatc ggcgaagatt
    12781 tgggtattct cttccaactg gccggtctgc tggaaaagta tattgaggaa ctccgcctct
    12841 atcgccccac gaggatcgtc gaggagtttg cccggacgat tcgcaaggag atcaatttca
    12901 acctggaggc ctcccatgcg gaacgcttcg cccggcagtt ccggggcaac cccgccgttt
    12961 atgtccccag ggtcttccgc catgcctcga cggagcggct cctcaccatg gagtacatcg
    13021 agggaatcaa gatcaacgat atcgctcttc tggaggccca gggctgcgac cgcgccttgc
    13081 tggcggcccg gggaacggag cttatcctgg aacaggtctt catccacggg ttttttcatg
    13141 ccgatcctca cccggccaac atgtttgtcc tgcccggcaa cgtgatctgc accctcgatt
    13201 tcggcatgat ggggcacctg gacgcccggg accgggaaca cttcaccgag gtcatcatgg
    13261 gctatgtctc ccgggacgtc cccaagatca ccgatgccgt tctgaagatc gtcgagttcc
    13321 agcaggaacc ggaccggcgt cttctggagc gggacattgc cactttcgcc gatcttcatc
    13381 tctataagcc cctgaaggat gtccgggtcg gcgagattct ggaggatctc atcgaactca
    13441 tctcccggca tcgcctccga ctgcccccgg atatcttctt catgattaag gccatgaccc
    13501 aagtggaagg catcggcctg cagttggacc cggatttcga tattaccgtg aaagtccgtc
    13561 ccttcatcaa gcgcctcatc acggagcgtc tccagccgaa acagcttctc cttaaggcct
    13621 tcgattccac tgccgcgctt ttgggacatc tccgggacct tcccgaggac ctcctcagca
    13681 ccctgcggca ggtccgagaa ggcaagatac aaatcggttt cgaacaccgg gggctggaac
    13741 gcctcatctt cgagctgaac cggtcttcca accgcctggc ctttgccctg atcatcgcgg
    13801 cgattgtggt tggttcatcc ctcctcctgg ccgccgagat cggtcccgcc gtcttcgggc
    13861 tgtccatcat cggtctcctc ggtttctccc tggcgggcct ccttggtcta gccttgttga
    13921 ttttcattat tcgttccgga atactgtagg ttgtagaatg ccggaacttc ccgaagtaga
    13981 gaccctcatt cgccagctca gaaacgtgat caccacggag cggattcgcg cggtgcggat
    14041 ctacgatgaa aagctcaaat atcttcccga agtgatcggc cggcgggtcc gtgcagtccg
    14101 gcgggccggc aaaggggtgg aaatagatct gggtggtccg gtgatccaca tccatctccg
    14161 gatgtccggg cggctctact ggcggcccgc cggcgatatc atcccttctc atgcccgctt
    14221 cagcatcttt ttcgacaccg gcgagatcct ctgcatcgac ccccgccgtt tcgccacgct
    14281 gaccttgtat tgcggccccg ccgccctccg gaaccggtct gtgaacctgt tggccggcat
    14341 gaacgtcccg gagatctgcg cccgggccgg cgaccggcgt ctgcccgtca aggcctttct
    14401 cctggaccag caagtcatgc cggggatggg aaatatctat gcctgcgaga tcctattcgc
    14461 tgctgccgtc agtccctggc gccgcactgg cgatgtcacc gccgtcgaat ggggacggat
    14521 cgcggcggcg gccggcagca tcctcaaaga ggcggttttt tgccggggaa cgagcatttc
    14581 cgactggcgg gatctcttcg gccgtccggg cgagtatcag cggcgattga aggtctatga
    14641 tcgggaaggc aagatctgct tccgctgtcc ggcccgcatt gagcgccgga aactcagcgg
    14701 tcggggtaca tattactgcc ccggttgtca gaaatgaaag cgtcaatcac gattctttac
    14761 ggcttcatca gaaatcactg tcggcagtcg ccgtgccttc agccagcacc aggccgccgc
    14821 cagcaatccg gggttgcgta gaccgacctt cgtcctgatc ccggccctgc ggaccgcggc
    14881 cgcatggagc aggtcatcga aatcggtaaa cgacagggtt ttcatcttgg cgatcaacat
    14941 ctcttccaaa acgagtttat gcttaatcaa ggagagcagt tcccggcgat atgcgccaac
    15001 cccctgcttt cccttgctta gtgcttctac cgcccgaatg ccgctgatcc gagccagatc
    15061 gattccacca ccgtggaggg gtgaggtgag accggccgcg tctcccacca ggacaatgcg
    15121 accgtaaacc agcctcggga gcatgtgggt gggaataagg ccgccgccct tccggagcac
    15181 ccgttccgga cgcaacgact cgcggcccat aattttcgtg atcagtcctt tcaagtcgag
    15241 gggaaacccg tcttccccgc cgcgggtgaa aaccaccccg acgttggcga cgccggcgct
    15301 cttcgggaat atccaatagt atcccggtag gcattccgcc ggcgcccagg acaggaatcc
    15361 caccttgatg gtggcggcgg aaaagagcgc ggagaagtcg ccggcaacgg tgtattgata
    15421 ggcgaggagg gggcggcggc ggtattcgcc gttaaaaccg tatgcccgtg aggtgacgga
    15481 gggcgccccg ctggcgtcga tgacccaatc gtaacgggaa acaagttccc cgacgccggc
    15541 cggggttatc ttctctccac atctgacgtc caccctaagg gccaccgcct gggccgctaa
    15601 ctgccgctgc caggtgcggc gatccaccat ccagaggtgc cggtaatttt tgatcggcag
    15661 ccggtaggta ccccgggcgg aaaagaggat agtccggacg tcgtaaaggc gtccggcgcc
    15721 gggcgggggg agcattccca gggaatcgaa gatgcattca ccgcacacga tgcggtcgcc
    15781 gacgtccccc ttttcgaaca ggacgacctt catgccccgg cgcgcggcgg caatggcagc
    15841 gtgaagtcct cccggtccgg cgccgattac ggccagtgtc ggcgccgccg aggtctttcc
    15901 ctgcccgctt cccttctcca tgaaccggac atgtctcctt atcccccgcc catgggaggc
    15961 gaaagagatg agtattgcct gccgtcgcgg ccggagggat aaccaactcc gccgccgcct
    16021 atccggaggg caggcatcct accgggggca tttcccctcg taatgatccc agacatcgca
    16081 gcttcggccg tctttcagga gacagcttcc ctgttccgtg ggggtattgg ctgccccggt
    16141 gacgatgtag cttccacccg tgatgacgca atattgagcc gccggagtta ccagacccgt
    16201 tatctttttc ccgcccggag gacattctcc ccgaaaaagc gcccattcct cacaccggcg
    16261 atcgtcttcg aatacacaga tcccgtattc accgccatcg cctctttttt gaatgatcaa
    16321 ccgaccaccc ttctccaggc agtttaccga cgcgggattg gccatggccg ccttgacgtc
    16381 atgatttctt tgaacttccg cccctgggga ttggcacccg caaaggagcg ccgccacaac
    16441 cagcagggcc gttcccgtcc atgatacaac cggggatcga tctcttgttg accatagctt
    16501 cagaacaagg ttcatattag tacttattcc tttgaaatcg ttaccttaag gcttccgcgg
    16561 ttgtcgtgcc gataaaaggg gtagtcattg atgaccagat atagcgtgcc actggcaggc
    16621 atgataattt cgttccggat gccgatggga aaaacatggc ggctcacccg gcccaacagc
    16681 gttccaaacg gcgttccggg caactccttc ttgtgatctc ccgcccggtg caccacttcc
    16741 cccggatgat tgccatgccc ctccggaccg gcatcgggcc atctttccct ttcgtcgtga
    16801 ggagctaccg cccaggcgcc ttcgcttcgg aagaaaacgg tttgcccttt aaccacgcga
    16861 atccccgtgt cgatccaatc cgatttggca tccacatcca ccgtcgccgc tggcaacttg
    16921 tgatgcgcct ggccaaagca ggcgctcaac agcatcgcga gcgggatcag gaccgataaa
    16981 tatctcgttt tttcatgccg ggtcatctct tggaaccatg taaaattagt caattgtaat
    17041 gtcatgtaaa agcacaaccg cacgggtgtc aagcgttttt ccgcgccctg cgcatctttc
    17101 gttgtgtcgc ctttgaactt gatggtcctt tgccgatgat attcatccaa actaatgggg
    17161 aaatgatgca aaacccactc tgggtttgga taaccgtgta aagacatgct caaaacaacg
    17221 catgcgccta gtggtttttc cgacacgtat gtttttcgcc gcctataggc cgctccccat
    17281 aagaaaaccc ctttccatcg aatcggaaag gggttttctt cgttcttgcc ggtgcggaga
    17341 tcgttacttt acatggaccg gcgtgcaggt gggatcgacc cagcccttcc agattttctt
    17401 ggtctctttc gccttcttca tgatcctttc ctcctctctc gggttcttta ttgcggctac
    17461 tgccgctttc tttccttctt tcgatagtag agtaaatgat ctcgggtttt ttaacaatcg
    17521 gaggcgggat gattgtggaa tgggaattta gatggcgggg agataaacgg ccgccgcttc
    17581 cacccatcgg tttttgtctt aggggggtgc ggcaagatca cgccccggag gggcgccgga
    17641 gcgcgtataa gtatacattg ctcatggcgc gtcggtaatg tttacttctc cccccgctca
    17701 tcgtctttag atgaatcatg ctgactgtcc gccgaccggc aggacggttt cagtcaggtg
    17761 gatattccca gccggggaag gatatgggcc tggagcacgt accagacggc tatgaccaaa
    17821 attatcgtta atagctcttt cgtatcacaa gcctcatttg gttacagtga actaccaccg
    17881 agcttgctcg gtggtagttc acttctgcat tggtttttaa gttatccaaa atggaaagcg
    17941 cgacatccat ggcccctgac tgaatgagcc acgatattcg tcaacataga gtattgaata
    18001 gggtcatagc cgccatagct catggcaatc gttaccctct ttccggccag agcatcttcc
    18061 atgtaaaaga aatgggagct tagagcaatt atctaaaccc ccattatcac taagaaataa
    18121 aatttcaatt tggtgccgaa gccgggattt gaacccggac aggcgtacac
//
LOCUS       DAOSMF010000029        17162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_3974_length_17162_cov_23.196294, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000029 DAOSMF010000000
VERSION     DAOSMF010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 17162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17162
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3974_length_17162_cov_23.196294"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(324..827)
                     /locus_tag="PLT63_04910"
     CDS             complement(324..827)
                     /locus_tag="PLT63_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOD98098.1"
                     /translation="MIGRNLLPSLWNSAATPAAAADDHPFFSIQREMNRIFDDFFRGM
                     EVSPRPTGDRLNRFVPSLDIRENDKEVTIKAELPGMDEKDVEVLLTENTLTLKGEKKE
                     EKEDKGKDYYHMERTYGSFQRVIPLPEGIDMKKAEAKFKKGVLSINLPKTEATKVKGK
                     KIEIKAA"
     gene            complement(899..1306)
                     /locus_tag="PLT63_04915"
     CDS             complement(899..1306)
                     /locus_tag="PLT63_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOD98099.1"
                     /translation="MEKGALDLQKQQAQNPLETERTRNRKVYVPKVDIYETKDAMVLI
                     ADMPGVDDKSVDITLEKNVLTITGNVESPAYEGKTLAYAEYDTGDYQRAFTISDEVDQ
                     DRIEAAVENGVLRLTLPKVEKAKIKKIAIKAEK"
     gene            complement(1325..1693)
                     /locus_tag="PLT63_04920"
     CDS             complement(1325..1693)
                     /locus_tag="PLT63_04920"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOD98100.1"
                     /translation="MNRLFSVSTHPFVQVYPAVNLWVGEHEAVITAELPGVNPEQIDI
                     SALGDNITISGAREQEVLKENETYHRKERNGSRFSRTLQLPFRIDAAGIEARYEKGIL
                     KVTAPRAEEDKPKKIAIKAA"
     gene            complement(2125..2784)
                     /gene="atpB"
                     /locus_tag="PLT63_04925"
     CDS             complement(2125..2784)
                     /gene="atpB"
                     /locus_tag="PLT63_04925"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOD98101.1"
                     /translation="MHSLSFLQLILPGVPTYITYTWFVMALLALFSFLATRRLSLTPG
                     GFQNSMEVFIKAIDNLLIDTMGTHGKRFFPLIATLGFFILTSNLIGLIPGLESPTANL
                     NTTVAMALVVFFLTHIVGVQIQGMKYFKQFLGPVWWLTPLMLPIELVSHISRPVSLSM
                     RLFGNIEGGHIVLFVLFILVPLLVPLPILVLKLLISFIQTLVFMLLSMMYIAGAMEEH
                     H"
     gene            complement(2788..3183)
                     /locus_tag="PLT63_04930"
     CDS             complement(2788..3183)
                     /locus_tag="PLT63_04930"
                     /inference="COORDINATES: protein motif:HMM:NF015834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HOD98102.1"
                     /translation="MNLTGKDPLQQRLEIANWVVLAVFVLAGALIFSLKFTLGVLLGG
                     LISIVNYHWLCRDVKKVFAHLTDRAKSRIMFKYYIRFGITAVALYFIVSSGIVEVVGL
                     LIGLSTVIVNIVITAVMALSKKNCVEEVN"
     gene            complement(3143..3427)
                     /locus_tag="PLT63_04935"
     CDS             complement(3143..3427)
                     /locus_tag="PLT63_04935"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HOD98103.1"
                     /translation="MDKEGRKAYVQMAYASSIGFAFVLLIFGGLFLGNWLDKKMGTSF
                     FFTVLLFIMGVIGGFRNIYVLIGKSFQDDKIIIKSIKSESHRKRPAPAKA"
     gene            complement(3487..4431)
                     /locus_tag="PLT63_04940"
     CDS             complement(3487..4431)
                     /locus_tag="PLT63_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HOD98104.1"
                     /translation="MKNKRITITGGKGFLGTHLVKGFRDRGYENILIADLPDYNLVRG
                     EDVRRLYAETKPDIVIHLAAKVGGIGFNREHPGALFYENIMMGAQMIHEGYRSGIEKF
                     VALGTICAYPKFTPVPFREDELWNGYPEETNAPYGLAKKMMLVQSQAYRQQYGFNAIF
                     LLPVNLYGPGDNFDPRSSHVIPALIKKCVDARRAGEETVTVWGTGKATREFFYVEDAA
                     EAIIMATERYNRPEPVNIGAGFEISIRELAELIVALTGYAGRIVWDTSQPDGQPRRML
                     DTTRAWEEIGFRSRTDFREGLRRTISWYENEGIKRNYA"
     gene            complement(4525..5553)
                     /gene="gmd"
                     /locus_tag="PLT63_04945"
     CDS             complement(4525..5553)
                     /gene="gmd"
                     /locus_tag="PLT63_04945"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOD98105.1"
                     /translation="MKKAFITGITGQDGSYLAEFLLAKGYEIHGLIRRASTFNTDRID
                     HLYKDFHDPEARIYLHYGDLAVSGQLTDLLNDIKPDEIYHLGAQSHVRVSFDMAEYTG
                     DVTGLGTLRLLEAIRKSGIKSRFYQASSSEMFGAAPPPQSEATPFQPQSPYAAAKVYA
                     YYIVKNYREAYGIFAANGILFNHESPRRGETFVTRKITRAATRIKLGLQEKLFLGNLE
                     AKRDWGFAGDYVEAMWLMLQQDRPDDFVIATGETHSVREFAQRVFEKLNLDYEKYVAI
                     DRRYFRPTEVDILLGEASKARNLLYWRPRVSFEGLIDMMIAADMESARKEKTLCEAGY
                     EIGNHNQL"
     gene            complement(5668..6684)
                     /locus_tag="PLT63_04950"
     CDS             complement(5668..6684)
                     /locus_tag="PLT63_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HOD98106.1"
                     /translation="MDKILVTGAAGFIGFHLCRKLLATGRNVVGIDNLNPYYEVRLKE
                     DRLRLLEEDRSFRFFRLDIADREDLADLFAREEPPVVVHLAAQAGVRYSLTNPYAYLD
                     SNLLGFLNILEGCRHHRVSHLVYASSSSVYGGNTRLPFSVHHNVDHPVSLYAATKKAN
                     ELMAHSYAALYGIPCTGLRFFTVYGPWGRPDMALFLFTRAILAGEPIDVYNEGRMERD
                     FTYIDDIIEGVFRIMARPPDPDPSWNGGNPDPAGSFAPYRLYNIGNHQPVALLDFIAA
                     LEKNLGKKAKMRLLPMQPGDVPATCADVEDLAAAVGFSPATTIATGIARFVEWYKKYY
                     LNKK"
     gene            complement(6789..8072)
                     /gene="hemL"
                     /locus_tag="PLT63_04955"
     CDS             complement(6789..8072)
                     /gene="hemL"
                     /locus_tag="PLT63_04955"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HOD98107.1"
                     /translation="MKDRSQALFAQAERLIPGGVNSPVRAFKAVGGAPVFMNAGRGAR
                     ICDVDGNEYIDYVMSWGPLILGHTHPAVVAAISAQAEKGTSFGAPTPLEVELAGLIIE
                     AFPNLEMVRMMSSGTEAVMTAIRLARAFTGRDRIVKFAGCYHGHSDALLARSGSGVAT
                     LGLPDSPGVPGSLAELTITIPFNDPGAVAAVFAKHGPDIAAVIVEPVAGNMGVVLPQP
                     GFLSRLRELTRAHGSLLIFDEVITGFRLAWGGYPGGGGIDPDLTCLGKIIGGGLPVGA
                     VGGKRAVMEQLAPLGPVYQAGTLSGNPLAMAAGLATLTILRESTECYEAMKRYAFSLC
                     EEMQGLFNRKGMPVTINRLDSMFTIFFTAGPVCDLDTARRSDTELFARFFRGMMSNGV
                     NPPPSQFEAWFVSLAHDLLDAEKTLEACARTLETM"
     gene            complement(8069..8533)
                     /locus_tag="PLT63_04960"
     CDS             complement(8069..8533)
                     /locus_tag="PLT63_04960"
                     /inference="COORDINATES: protein motif:HMM:NF037131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98108.1"
                     /translation="MFSNREKALARRLQGDIPLVSRPFEEIGAATGQTEQEVRAFIDR
                     LILQGVIRRFGAVVRHREVGYTHNAIVAWSAPSERIEEAGRYLAAQREVTHCYERTPP
                     FLGRYNLFSMIHGRGEDAGAMIGRFARKMEIEDYLILRSEEELKKTSMEYFT"
     gene            8781..9155
                     /gene="atpE"
                     /locus_tag="PLT63_04965"
     CDS             8781..9155
                     /gene="atpE"
                     /locus_tag="PLT63_04965"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HOD98109.1"
                     /translation="MKKGLVRGILAVTLVLVVAPLAMAAEAAAPGGAVDYTKAIILGC
                     SVLAAGLAMGIGAVGAGLGLGQATSGANNAVGRNPEAQGKIMLTMMVGMAMTESVAIY
                     ALVVSLVILYANPLLKFAGIGG"
     gene            complement(9229..9630)
                     /locus_tag="PLT63_04970"
     CDS             complement(9229..9630)
                     /locus_tag="PLT63_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HOD98110.1"
                     /translation="MAFALLADFVVVAHLLFILFVVAGGFLALRNVKWAWLHVPAAVW
                     GVLVEWSGWICPLTPLENRLRRLGGGPVYSSDFVERYIVSLIYPEALTRNEQVLLGLA
                     VLVINGGIYGWLAVRMWRSRGRDGREVALKK"
     gene            complement(9645..10298)
                     /locus_tag="PLT63_04975"
     CDS             complement(9645..10298)
                     /locus_tag="PLT63_04975"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HOD98111.1"
                     /translation="MQKRNVLMISVLLILFFFPPPAATQEFKEIPLVKPQMDSGRLLM
                     HVLKDRRSSREFSNRKLPIRVISNLLWSACGINRPESGKRTVPSAGNWQEIDVYVATA
                     KGLYVYDARRHRLHPVLGRDIRALTGSQSYVKDAPIDLVYVADYTRMGNAAPEVKDLY
                     AAIDTGFISQNVYLYSASEGLATVARGTMDRTALGQAMMLRPDQRIILAQTVGYPKK"
     gene            10550..11215
                     /locus_tag="PLT63_04980"
     CDS             10550..11215
                     /locus_tag="PLT63_04980"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOD98112.1"
                     /translation="MNRPDDNYWGRYARTYDGDTDYVVGRGLRRALYGRLRRETELGV
                     LLECGCGTGYYTRAVAPRSERIVAMDLSGEMLAVAKTRLSVMPHVSFLRGDCRQRTFL
                     PTSFDTVLMANILNNLSEPLPALCEVHRVLKYGGALIAIAYTDYHADALDKIEIGKRY
                     FERFGLPPPGGLRNYSPRQLASLIRQAGFMNVDIEVLGGTVKVLYARSVKSYVTDDCP
                     TDK"
     gene            complement(11356..13143)
                     /locus_tag="PLT63_04985"
     CDS             complement(11356..13143)
                     /locus_tag="PLT63_04985"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOD98113.1"
                     /translation="MPEKHLALMVPKQAKKYGDRTALRYREHSAWRDISWREVEDRVR
                     SAAKGLLELGIPEGGMVGIFAHNCPEWTIADFAILSVRGVSVPIYATNTAKQAEYIVN
                     ETEIAVLFVGGAEQYRKAVSISATSIFLKKIIVFNSSVPLADPGGRDVYFLDFLVSGR
                     QAAQDLELESRLKKADMNDLATLIYTSGTTGEPKGVMLHHSNFYFSFQAHNKRLSVSD
                     RDVSMCFLPLSHVFERAWTYFALGSGMVVNYCDDTGKIVEYLQEAQPTIICAVPRFYE
                     KIYGAIFEKLEKAPASRKNLFLWAVELGEKVYLRTKEKESIPWDLKLKHRIAEKLVLE
                     KLRKIVGGRIRFFPCAGAPLSRHIEEFFHSVGIPIKYGYGLTETTATVTCHEEYHYRP
                     GTVGKPIYGVDVKIAPDGEILVRGETVTKGYYKKPEATSEVFTDNWFRTGDAGVLEDG
                     YLTITDRIKDLMKTSGGKYIAPQLIETTVGKDHFIEQISIIGDERKYVSALIVPTFTA
                     LEDYARSRNIDFASREELIGHPEIVKFYHDRIEANQKELAGFEKIKKFKLLAKPFTQD
                     DGEMTPTMKLKRKVINEKYRDIIDGIYRE"
     gene            13355..14551
                     /locus_tag="PLT63_04990"
     CDS             13355..14551
                     /locus_tag="PLT63_04990"
                     /inference="COORDINATES: protein motif:HMM:NF013383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HOD98114.1"
                     /translation="MDFDTTRIAIIGPGRLGTSFAYKFGRDNKRVTIYYHDAEVCRAI
                     NRDHLNPKHLTEDLAKRLGGMEQVPRLAPKVYATNNLERVIEDNDIIVLCLTMNKLPE
                     FLNYLKPLIERKSGDTCLVSPIKGLTSDEITKELITPSQLINSNLGSLNYKYEIVCIG
                     GPFFDVDIALGNPVCVTVAGKRGSANLIREELIRHNRRELTSYYNFDIVGVEACGSLK
                     NVVANIKGVTDSLDLGDSIPGTIFARAGVEIRSLSMLLGGSFQAFQSQAGVGDMYVTV
                     SSMASKNYRYGKYFYELYSGNPIETNLKVLARIDGTPEGPNTIRNVHRYLEKKNMYSP
                     LFHCAYKIFNEGGTRRDIKEMIIQACQFDKRVKEFIGPVSRFFYRVIPNFWYRRHKGM
                     MSNWDV"
     gene            14919..16280
                     /locus_tag="PLT63_04995"
     CDS             14919..16280
                     /locus_tag="PLT63_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HOD98115.1"
                     /translation="MEPMKVLLEDQEIPRQWYNIQADLPTPMRPPLHPATGKPVHPDD
                     LAPIFPMNLIEQDATTQRWIDIPEEVLEKYLIWRPSPLCRARNFEKLLDAPVKIFYKN
                     EGASPAGSHKPNTAIPQAYYNKAFGIKRLSTETGAGQWGSALSMACQMFGLECRVFMV
                     RVSYEQKPYRRMMMNVWGAECIPSPSNITEAGRNVLAEHPDSPGSLGIAISEAIEDAV
                     SHPGSRYSLGSVLNHVLLHQTIVGLESQKQFAKLGLYPDVVMGCAGGGSNFAGISLPY
                     VRDRIHGKDVRIVGAEPTACPTLTRGPFAYDFGDLAKKTPLLPMYTLGHDYVPAPIHA
                     GGLRYHGMAPIVSHLLKENLIEARAYHQLDTFQAGVMWARSEGFIPAPETNHVIAAVV
                     DEARRAKEEGKEKVILFNWSGHGMVDLASYEAYLTGKLIPHELPDEEIDRALRAIEGF
                     PKP"
     gene            16453..16722
                     /locus_tag="PLT63_05000"
     CDS             16453..16722
                     /locus_tag="PLT63_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98116.1"
                     /translation="MSEITRYDDREIRCPKLGHELTFAYCRREGGELPCFRVIRCWEG
                     FFPVESFLRESLSPDAWETFRRHTPPDKLTSLLDLIAKAQQRRGG"
     gene            16827..>17162
                     /locus_tag="PLT63_05005"
     CDS             16827..>17162
                     /locus_tag="PLT63_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOD98117.1"
                     /translation="MKKREFQPLADRMRPRRLAEYVGQTHLLGKDGLLRRSIEEDRLF
                     SMIFWGPPGSGKTTLARVIAQETSSHFTSFSAVLSGVKEIRAVVEAAVALRDEQGEKT
                     ILFVDEIHRF"
BASE COUNT         3560 a         4762 c         5051 g         3789 t
ORIGIN      
        1 cccgccgccg cgtcgggaag ggaaagaatc cccaccatga ccaggacaat caggaagaaa
       61 actgatctac cggccttgtc ccggtaatct gtgattattc tgagcataat tcgctttttt
      121 ggtcccggac cggtcgccgg ctgtctcgcc gggaaataat ctttgtgttt cctgacggga
      181 agataggaaa agatcgcggt gttgtcaatg ccattgtttt atggagagtc agatgcctgg
      241 gaggaataag cggatctttc atcctcccgg cgggattgcc gggaggatga aagacggtga
      301 gggtgatatg gtgagggtga tggttatgcc gccttgattt cgattttttt acccttgacc
      361 ttggtggctt ccgttttggg caggttgatg gagaggacgc ccttcttgaa tttggcctcg
      421 gccttcttca tgtcgatccc ctcgggcagg ggaattaccc gctggaagga cccgtaggtt
      481 cgctccatgt ggtaataatc ctttcccttg tcttcctttt cctctttttt ctcacccttg
      541 agggtcaggg tgttttccgt caggagcacc tccacatcct tttcgtccat tccgggcagt
      601 tccgccttta tggtcacttc cttgtcgttc tcccgaatgt ctagggaagg gacgaaacga
      661 ttcaagcgat ctccggtagg ccgggggctc acctccatgc ccctgaagaa atcgtcgaag
      721 attcggttca tctctctctg gatgctgaag aagggatgat catcggccgc cgcggccggg
      781 gtggcggcgg aattccagag cgacggcaaa agatttcttc cgatcatagc tgttcctcct
      841 ttcctgatat tttatcggca cgccgttgac cgcgaccggc acggtaacag gacggcgttt
      901 acttttccgc cttgatggcg atctttttta tcttggcctt ttcgaccttg gggagtgtca
      961 gccggagaac gccgttttcc actgcggcct cgatgcggtc ctgatccact tcgtcggaaa
     1021 tggtgaaggc ccgctgatag tctcccgtgt cgtactcggc ataggccagg gtcttgcctt
     1081 cataggccgg ggattccacg ttgccggtga tcgtcaggac attcttctcc agggtaatat
     1141 ccaccgactt atcgtcaacg ccgggcatgt cggcaatcaa caccatggcg tctttcgttt
     1201 cataaatatc gaccttcggc acatagacct tccggttcct ggtccgttcc gtttccaggg
     1261 gattttgcgc ctgctgtttc tgcagatcca atgctccttt ttccatatca ccaacctcct
     1321 ttccttatgc cgctttgatg gcgatttttt tgggcttgtc ttcctcggcc cggggcgccg
     1381 tcaccttgag aatccccttt tcatatcggg cctcgatgcc cgcggcgtca atgcggaagg
     1441 ggagttgcag cgttctggaa aaccggctgc cgtttctttc cttgcggtga taggtttcat
     1501 tctccttcag cacttcctgt tcccgggcac cggaaatggt tatattgtct cccagtgcgg
     1561 aaatgtcgat ctgctccgga ttcacgccgg gcaattccgc cgtgatcacc gcttcatgtt
     1621 caccaaccca gagatttacc gccggataga cctgcacgaa gggatgggtg gaaacggaga
     1681 acagccggtt catctcattc cgcaaccgcg acatttctgc atacggatcc aataagcgac
     1741 caaatctcca tgctcctgtt ccaaacataa tctctcctcc tttcctcgtt tatcgaaaat
     1801 gttctttctt atcagtttct caaagggtgc acgaacccct ctatccaatc ttaacatctt
     1861 gttatgaaag ggttatttca tggcggcctt aatatattca ccaaatggag cttgtcaaga
     1921 ggggtagata cgtcggacaa cctgcctgtg ggacgcaccc gaaaggcgcc atcgggcggc
     1981 ctggaaaacc ctggcgctgc atgagcgaat cccgggtaca gtggggtgat gggacaaaaa
     2041 aacggggtcc cgttgaggga ccccgccggc attttaaaga cggttcggcg ccccctttcg
     2101 gacggcgcct gcgtgcggaa tttatcagtg atgttcttcc atggctccgg cgatgtacat
     2161 catggacagg agcatgaaga ccagggtctg gatgaaggag atcaggagct tcaggacgag
     2221 gatgggtagg ggtaccagca gcggcacgag gatgaagaga acgaagagga cgatatggcc
     2281 gccttcgatg tttccgaaca gacgcatgga cagcgaaacc ggacgggaga tgtggctgac
     2341 cagttcaatg ggcagcatca ggggcgtgag ccaccaaacc ggtcccagga actgcttgaa
     2401 atatttcatg ccctgaatct gcacgccgac gatgtgggtg aggaagaaga ccaccagggc
     2461 catggcgacg gtggtgttaa gattggccgt gggggattcc agtccgggaa tcagcccgat
     2521 caggttggaa gtgaggatga agaatcccag cgtggcgatc aggggaaaga atcgttttcc
     2581 atgggtgccc atggtgtcga tgagaagatt gtcgatggct ttgatgaaga cctccatgga
     2641 attctggaac ccgcccgggg tcagggaaag gcgccgggtc gccaggaaag aaaagagggc
     2701 gagcagggcc atgacgaacc aggtataggt gatgtaggtg gggacaccgg gtaatattaa
     2761 ttgcaggaaa gacagactgt gcatcttcta attgacctcc tcaacgcaat ttttttttga
     2821 aagagccatg acggcggtaa tgacgatatt gacgatgacc gtggaaaggc cgatcaacag
     2881 gccgacgact tcgacgatgc cgctggatac gataaagtac agcgccacgg ccgttatccc
     2941 gaagcggatg tagtatttga acatgatccg ggacttggcg cggtccgtaa ggtgggcgaa
     3001 gacctttttg acgtcccggc agagccagtg atagttcacg atgctgatga ggccccccag
     3061 gagaacgccc agggtgaatt tcagggagaa tatcaaggcg ccggcgagaa cgaatacagc
     3121 gaggacgacc cagttggcga tttcaagcct ttgctggagc gggtcttttc cggtgagatt
     3181 cactttttat actcttgata attattttat cgtcctgaaa tgacttgccg atcaggacgt
     3241 aaatgttcct aaatccgccg atgacgccca tgatgaagag caggacggta aagaagaagg
     3301 aggtgcccat cttcttgtcc agccagttgc cgagaaagag acctccaaag atcaacagga
     3361 cgaaggcgaa gccgatgctg ctggcgtatg ccatctggac gtatgctttt ctgccctctt
     3421 tatccatcga cgcagcgcct cttaacacga taggggtggg aagtcaacaa aaaatgacgg
     3481 ccccgttcac gcatagtttc tctttatccc ttcgttttcg taccaggaga ttgtccggcg
     3541 gaggccctcc cggaagtcgg tccgagaccg aaaaccgatt tcttcccagg ctcgggtcgt
     3601 atcgagcatc cgccgcggct ggccgtcggg ctggctggtg tcccagacga tccggccggc
     3661 atagccggtc aacgcgacga tcagttccgc cagctcgcgg atggagatct cgaacccggc
     3721 gccgatgttg accggctcgg gtctgttgta cctttccgtg gccatgatga tggcttcagc
     3781 ggcgtcttcc acatagaaga actcccgggt ggccttgcct gttccccaga ccgttacggt
     3841 ctcctcccct gctcttctgg catccacgca tttctttatc agggcgggga tgacatggga
     3901 ggaacgggga tcgaaattgt ctcccggccc gtagagattc acgggcagga ggaatatggc
     3961 gttgaagccg tattgctggc ggtaggcctg cgactggacg agcatcatct tcttcgccag
     4021 gccgtaaggg gcgttggtct cctcgggata gccgttccag agttcatctt cccggaaggg
     4081 tacgggggtg aatttgggat aggcgcagat cgtgcccagg gccacgaatt tctcgatccc
     4141 gctccggtat ccctcgtgga tcatctgagc gcccatcatg atgttttcat agaagagggc
     4201 tcccggatgc tcccggttga agccgatacc ccctaccttg gcggccaggt ggatgacgat
     4261 gtcgggcttc gtttcggcat acagccgccg gacatcttcc ccgcggacca gattgtagtc
     4321 cggtaaatcg gcgatgagga tattttcata gccccggtcc cgaaacccct tgaccaggtg
     4381 ggtgccgaga aaccccttgc cgcccgtgat ggtgatgcgt ttgtttttca tcccgctgct
     4441 tccgctccct tgggttgctg aatcgccgcc accgctccgg acgcgccgtc aagaagatcc
     4501 gcgattggcc cccggcgccg cgattcagag ctggttgtga tttccgattt cataccccgc
     4561 ctcgcagagg gtcttttctt tccgggcgct ctccatgtcg gcggcaatca tcatgtcgat
     4621 gagaccttca aagcttaccc gggggcgcca gtacaggaga ttccgggcct tggacgcttc
     4681 cccgagcagg atgtccactt ccgtggggcg gaaatatcgc cggtcgatgg cgacgtattt
     4741 ttcatagtcc agatttagtt tctcgaagac cctttgggcg aactctcgca cggagtgagt
     4801 ctctccggtg gcgatgacga aatcgtcggg gcgatcctgc tggagcatga gccacatggc
     4861 ttccacgtaa tccccggcga atccccagtc ccgtttcgcc tccagattgc cgagaaacaa
     4921 tttttcctgg aggcccagtt tgatccgcgt cgctgcccgg gtgatcttcc gggtgacgaa
     4981 ggtctcgccg cgccgtggcg attcgtggtt gaaaaggatg ccgttggcgg cgaagatacc
     5041 gtaggcctcg cggtagttct tcacgatgta gtaggcgtaa accttagcgg cggcgtaggg
     5101 gctctggggc tggaaggggg tggcttcgct ctggggcggc ggggcagcgc cgaacatctc
     5161 gctggacgag gcctgataga atcgggactt gattccggac ttgcggatcg cctcgaggag
     5221 ccgcagcgtc cccagtcccg taacgtctcc ggtgtactct gccatgtcga agctcacccg
     5281 cacatggctc tgggcgccca ggtgatagat ctcgtcgggc ttgatgtcgt tcagaaggtc
     5341 cgtgagctgc ccggaaacgg ccagatcgcc gtaatgaaga tagatccgcg cctcgggatc
     5401 atggaagtcc ttgtaaagat gatcgatgcg gtccgtattg aaggtgctgg cccgacggat
     5461 cagcccgtgg atctcataac ccttggcgag gaggaattcg gccagatacg atccgtcctg
     5521 gccggttatg ccggtgatga aggccttttt cataagagta aatcgtctcc ctgtatgttg
     5581 tctgggcatc tccaggccgc cggaaatggc tctccgccat ggctgcccat ccgtgcctga
     5641 aggtccgata aaggttttcc tatcacatta tttcttattc aggtagtatt tcttgtacca
     5701 ctcgacaaaa cgcgcaattc ccgtggcgat ggtggtggcg gggctgaatc ccacggcggc
     5761 cgccaggtct tccacgtcgg cgcaggtcgc cgggacgtcg ccgggctgca tggggaggag
     5821 gcgcatcttc gctttcttcc ccagattctt ctccagggcg gcgatgaagt cgaggagggc
     5881 gacgggctga tgattgccga tgttgtacag gcggtacggg gcgaaactcc ccgcggggtc
     5941 gggattaccg ccgttccagg acggatcggg gtcaggcggc cgcgccatga tccggaagac
     6001 gccctcgata atgtcgtcga tgtaagtgaa atccctctcc atccgtcctt cgttgtaaac
     6061 atcgatgggc tcgccggcga gaatggcccg ggtgaagagg aacagggcca tatccgggcg
     6121 cccccagggt ccgtaaacgg tgaagaaacg caggcccgtg caggggatcc cgtacagggc
     6181 ggcgtaggag tgggccatga gttcgttggc ctttttcgtg gccgcataga gactgacggg
     6241 atggtccacg ttgtgatgca ccgaaaaggg caggcgggtg ttgccgccgt aaacggaact
     6301 ggaggaggcg taaacaaggt gggagacgcg gtgatgtcgg catccttcca ggatgttcag
     6361 aaacccgagc aggttgctgt ccagataggc gtagggattg gtcagggagt agcggacgcc
     6421 ggcctgggcg gcaaggtgca ccaccaccgg gggttcctcc cgggcgaaga ggtcggccag
     6481 gtcctcccgg tcggcgatgt cgaggcggaa aaagcggaac gaccggtcct cttccagaag
     6541 gcgtaaacgg tcctctttta ggcggacctc gtagtaaggg ttgaggttgt cgatgcccac
     6601 gacattccgg ccggtggcaa ggagcttccg gcacaggtga aagccgatga aaccggcggc
     6661 cccggttacc aatattttat ccatgattgt ctgctcccgt cggactggtt tttttatggt
     6721 cctattccat tttgcgcgtt ctcatcgcgg gcggtaggta gtctcggcct tcgcatggcg
     6781 gcggcgcttc acatcgtctc cagggtgcgg gcgcaggctt ccaaggtctt ttccgcatcc
     6841 aggagatcat gggccagaga aacgaaccag gcctcgaact gggagggagg tggattgacc
     6901 ccattgctca tcattcctcg gaagaagcgg gcgaataact ccgtatcgga gcgccgggcc
     6961 gtatccaggt cgcagacggg tccggcggta aaaaagatgg tgaacatgga atcgagccgg
     7021 ttgatcgtca cgggcatccc cttgcggttg aacagaccct gcatttcttc gcagaggctg
     7081 aaggcgtatc gcttcattgc ctcataacat tccgtgcttt cccggaggat ggtcagggtc
     7141 gccagacccg ccgccatggc cagggggttc ccggaaaggg tgccggcctg atataccggt
     7201 cccagaggcg ccagttgttc cattaccgcc cgttttcctc cgacggctcc gacgggaaga
     7261 ccgccaccga tgatcttgcc caggcaggtg aggtccggat cgatgccgcc gccccccggg
     7321 tatcctcccc aggcgaggcg gaagcccgtg atgacttcgt caaagatgag gaggctcccg
     7381 tgcgcccggg tgagttcccg gaggcgggaa agaaatcccg gttggggaag gaccaccccc
     7441 atattgccgg cgacgggttc gacgatgacg gcagcgatat ccggaccatg cttggcaaag
     7501 acggcggcga ccgcgccggg atcgttgaag gggatggtaa tggtgagttc ggccaggctt
     7561 cccggcacac cgggactgtc gggaaggccc agggtggcca cgccggaacc ggacctggcc
     7621 aggagggcat cgctgtggcc gtgataacaa ccggcaaact tgacgatcct gtcccggccg
     7681 gtgaaggccc gggccaggcg gatggccgtc atgaccgcct ccgtccccga actcatcatc
     7741 cggaccattt ccagattggg aaaggcctcg ataatcagcc ccgccagttc tacttcgagg
     7801 ggcgtcgggg cgccgaaact ggtccctttt tcagcctgtg ccgagatcgc cgccacgacc
     7861 gccgggtggg tgtggccgag aatcagggga ccccaggaca tgacataatc gatgtattca
     7921 ttgccgtcca cgtcgcatat ccgtgcgccc cgtccggcgt tcatgaatac cggcgccccg
     7981 cccacggcct tgaaggcccg caccggactg ttgacccccc cggggataag cctttccgcc
     8041 tgggcaaata gggcttgaga tctatctttc atgtaaaata ctccatagac gtctttttca
     8101 gttcttcttc gctccgcagg atcaggtaat cttcgatctc catcttcctg gcgaaacgtc
     8161 cgatcatggc gccggcatcc tccccccggc cgtggatcat ggagaaaaga ttgtaccggc
     8221 cgagaaaggg aggcgttctt tcatagcagt gggtgacttc ccgctgcgcc gccagatagc
     8281 gccccgcttc ctcgatgcgc tccgatggcg ccgaccaggc gacaatggcg ttgtgggtat
     8341 aacccacctc ccgatgccgg acgacagccc cgaatctgcg gattaccccc tgaaggataa
     8401 gccggtcgat gaatgcccgg acctcctgtt ccgtctgtcc cgtcgccgca ccgatttcct
     8461 caaagggccg gctgacgagg ggaatgtcgc cttggaggcg gcgggccaga gctttttccc
     8521 gatttgaaaa cacggcgctg attcgtttgt tcctctttct ctgcttttgt ggagcggatc
     8581 tatatcatat ttgccacgcc gtgcgccaaa tatttcttga caatcggatt gtggttttat
     8641 atgggatcgg cagaaatgtt acactggtgg ggagcgttgc ccagaccggt ggacggaaag
     8701 ggggtgcaaa ggggaaaaga aaaagtgtca attgagttgg acgtttggga gtcaaaaaaa
     8761 tcaatcaaag gaggaaagaa atgaaaaaag gtttggtaag gggtattttg gcagtaacgt
     8821 tggtattggt tgtcgctcct ctggcaatgg cggccgaagc ggcggcgccc ggcggagccg
     8881 ttgattacac caaggcgatc atccttggct gcagcgtcct ggccgccggt ttggcgatgg
     8941 gaatcggagc agtgggagca ggtctcggtc ttggtcaggc aaccagcggt gccaacaatg
     9001 ccgtaggcag gaaccccgaa gcccagggta aaatcatgct caccatgatg gtcggtatgg
     9061 ccatgacgga atcggtggcg atttacgccc tcgtcgtttc cctagtcatt ctgtacgcca
     9121 atccgctcct taagtttgcg ggcatcggcg gataacagac attcatatca ttatcgttat
     9181 catcgaaaag ggaaaagggg cactaaaaac agactccttt tccctttttt atttcttcaa
     9241 agccacttcc cttccatccc tcccccggga cctccacatc cggaccgcca gccagccgta
     9301 gatcccgccg ttgatcacga gtacggccag tccaagtaag acctgctcgt tgcgggtcag
     9361 tgcctccggg tagatcaggg agacgatgta tcgctcgacg aaatcggagc tgtaaaccgg
     9421 acctccgccc aggcggcgca gccggttttc caggggggtc agggggcata tccagccgga
     9481 ccattccacc agtacgcccc agaccgcggc ggggacatgg agccaggccc actttacgtt
     9541 ccgcagggcc agaaatccgc ccgcaacgac aaagaggatg aaaaggagat gagcgacgac
     9601 gacaaaatcg gcgaggagtg caaaggccat gatcctttaa cactttattt cttcggatag
     9661 cctaccgttt gggcaaggat gatgcgctgg tccggccgga gcatcatggc ctgaccgagg
     9721 gccgtccggt ccatggttcc ccgtgccacc gtcgccagac cttcggaagc gctgtaaaga
     9781 tagacattct ggctgatgaa acctgtatcg atggccgcat agaggtcctt tacctcgggg
     9841 gcggcgtttc ccatccgcgt ataatccgcc acgtagacca gatcgatggg ggcgtctttg
     9901 acataggact gcgagcccgt cagggcacga atatcccgac ccaggacggg atgcaggcga
     9961 tggcggcggg catcgtaaac atacaatccc tttgccgttg ccacatagac gtcgatttcc
    10021 tgccagtttc ccgccgaagg aacggtccgc tttcccgatt ccgggcgatt gataccgcag
    10081 gcgctccaga ggaggttgga gatgacccgg atggggagtt tgcggttgct gaattcccgg
    10141 gatgaacggc ggtccttcag gacgtgcatg aggagccggc cgctgtccat ctgaggctta
    10201 accagaggga tttccttgaa ctcctgggtt gcggccgggg gtgggaaaaa gaaaagaatc
    10261 agcaaaactg agatcatcaa cacgtttcgc ttctgcatga cgccctcctt tgccgctcct
    10321 tatactccag ccgcgccgat aaaggcaaac gttatttgcc ggatggggag taagggaggt
    10381 gttccgtctc cgggagccca gaaatcagtt ttactgttga cgagaagcac ggtatcattg
    10441 atataagaca tgatcgtggc caggttggag cgggaacgct cccgtatgac caacgcatcg
    10501 ccgaaccttt cggacgatcg aaataatttt tcccggagta ttggtgccca tgaacaggcc
    10561 ggatgataac tattggggcc gctacgcccg gacgtatgac ggggatacgg attacgtcgt
    10621 cgggagaggc ctgcggcgtg ccctttacgg ccgtttgcgc cgggaaacgg aactgggggt
    10681 cctactagaa tgtggttgcg ggaccggata ttatacccgg gcggttgccc cgcgatcgga
    10741 gcgtatcgtg gccatggatc tgtccggaga gatgctggcc gtggcaaaaa cacgactctc
    10801 agtaatgcct cacgtctcct tccttagggg tgattgccgg caacggacct ttctaccgac
    10861 aagtttcgat accgttttga tggccaatat cctcaataac ctttcggaac cgcttcccgc
    10921 cctttgcgag gtccaccgtg tcttgaaata cggtggcgcc ctgatcgcca ttgcctatac
    10981 ggattatcat gcggatgccc tggacaagat tgaaatcgga aagcgatatt tcgaaagatt
    11041 tggcttgccg ccgccggggg ggctgagaaa ttattctccc cgacagttgg cctcccttat
    11101 caggcaggct ggtttcatga atgtcgatat cgaggtcctg ggcggcacgg tcaaggtcct
    11161 ctatgcacgt tcggtcaaaa gctacgttac agatgattgt cccaccgata aataatcact
    11221 ttcccattat ttgaaataaa gcgcatcaac accggggatg tcctccccag tggggatgat
    11281 ctgttgggtt acggtcatcc ttccaggggc tccggggatt cgtaagcctg atcagaagac
    11341 aaggatggtt ccgcttcact cccggtatat cccgtcgata atgtcccggt acttctcatt
    11401 gatgaccttg cgcttgagct tcatcgtcgg ggtcatttcc ccgtcatcct gggtgaacgg
    11461 cttggccagc aacttgaatt tctttatctt ttcaaacccc gccaactcct tctggttcgc
    11521 ttcgatccgg tcgtgataga acttcacgat ctcgggatga ccgatcagct cctcccggga
    11581 tgcaaagtcg atgttgcggc tccgggcgta atcctccagg gccgtgaagg tggggacgat
    11641 aagggcggag acgtatttcc gctcgtcgcc gatgatggag atctgttcga tgaaatggtc
    11701 cttgccgacg gtcgtctcga ttagctgcgg ggcgatatat ttccctcccg aggtcttcat
    11761 gaggtccttg atccggtcgg taatggtgag gtagccgtct tccaggaccc cggcatcgcc
    11821 cgtccggaac cagttatccg taaacacctc cgacgtggct tccggtttct tgtagtagcc
    11881 cttcgtgacc gtctcccccc ggacgaggat ctcgccgtcc ggggcgatct tcacgtccac
    11941 gccgtaaatc ggcttcccca ccgtgccggg acggtagtga tactcctcgt gacaggtaac
    12001 cgtcgccgtt gtctccgtca gaccgtagcc gtacttgatg ggtattccca ccgagtggaa
    12061 aaactcctcg atatgcctgg aaaggggggc gccggcgcag gggaaaaagc ggatcctgcc
    12121 cccgacgatc ttgcggagct tctccagcac cagtttttcg gcgatgcggt gtttgagctt
    12181 cagatcccag gggatggact ccttttcctt ggtgcggaga taaacctttt cccccagttc
    12241 caccgcccac agaaagagat ttttcctcga tgccggggcc ttttccagtt tttcgaagat
    12301 cgccccgtag attttttcat aaaaacgcgg caccgcgcat ataatcgttg gctgcgcctc
    12361 ctggagatac tccacgatct tgcccgtatc gtcacaataa ttgactacca tgcccgaccc
    12421 cagggcgaaa taggtccagg cccgctcaaa gacgtggctc agggggagga agcacatgga
    12481 gacgtcccgg tcgctgacgg acaggcgttt gttgtgggcc tgaaaggaaa agtagaagtt
    12541 ggaatgatgg agcattaccc ccttgggctc cccggtggtt ccggaggtgt agatcagggt
    12601 ggccaggtcg ttcatgtcgg cctttttcag ccttgactcc aattccagat cctgggccgc
    12661 ttggcggccc gataccagga aatccaggaa gtacacgtcc cggccgccgg ggtccgcaag
    12721 ggggacgctg ctgttgaaga caatgatttt cttgagaaat atggatgtgg cgctgatgga
    12781 aaccgctttt ctgtactgtt cggcgccccc cacaaagagc acggcgattt ccgtctcatt
    12841 aacgatgtac tcggcctgct tcgccgtgtt ggtggcgtag atgggtacgg aaacgccacg
    12901 gacgctgagg atggcgaaat cggcgatggt ccattccgga caattatggg cgaagatccc
    12961 taccatgccc ccttcgggaa tgcccagctc cagcagaccc ttggcggcgc tacggacccg
    13021 gtcctccacc tcccgccagg aaatgtcgcg ccaggcgcta tgttcgcgat agcgcagggc
    13081 ggtgcggtct ccgtattttt ttgcctgctt ggggaccatc aaggccaggt gcttttcggg
    13141 catatccact ctcctttcta tgccgccttt ggtatcaggc cgcaatgggg tcggggtttt
    13201 caattctcct gtttttcttg gcaatataaa cggcaaagat ggagaagtca aaagcaattt
    13261 tattgataat actgaatatt atttgacgcc gtactgtttt tcccctataa ttgccgccgt
    13321 tttacctgtg gggggagaag tagccgtcgt gctgatggat tttgacacca cgagaatcgc
    13381 catcatcgga cccgggcgtt tggggacgtc attcgcctac aaattcggcc gggacaacaa
    13441 gcgggtcacg atctactacc atgacgccga ggtctgccga gccatcaacc gggaccatct
    13501 caatcccaaa catctgacgg aagatctggc caagcgtctg ggaggcatgg agcaggtgcc
    13561 ccgcctagcc ccgaaggtct atgccaccaa caatctggag cgggtcatcg aggacaacga
    13621 catcatcgtc ctgtgcctaa ccatgaacaa gctgccggaa ttcctcaatt acctgaagcc
    13681 gttgatagaa aggaaatccg gggatacctg cctcgtctcg cccatcaagg ggctgacctc
    13741 ggatgaaatc accaaggaac tcatcacccc ctcgcagttg atcaacagca atctcgggag
    13801 cctcaactac aagtatgaaa tcgtgtgcat cggcgggcca ttcttcgatg tcgatatcgc
    13861 cctggggaac cctgtctgcg tcaccgtggc gggaaaaaga gggtcggcga acctcatccg
    13921 ggaggaattg atccgccaca accgccggga actgacatct tattataatt tcgacatcgt
    13981 cggcgtcgag gcctgcggtt ccctgaagaa cgtcgtggcc aacatcaagg gcgtcaccga
    14041 ttcgctggac ctcggtgatt cgattcccgg gacgattttc gcccgcgcgg gggtcgaaat
    14101 ccgttccctg tccatgcttt tgggaggtag tttccaggcc tttcaaagtc aggccggggt
    14161 gggagacatg tacgtcaccg tgtcatccat ggccagtaag aactaccgct acggcaaata
    14221 tttctatgaa ctctacagcg gcaatcccat cgaaacgaac ctgaaggtgc tggcacggat
    14281 cgacggaacg ccggagggac ccaataccat tcgcaatgtc catcgctatc tggaaaagaa
    14341 aaacatgtac agcccccttt tccattgtgc ctacaagatc ttcaacgaag gggggacccg
    14401 gcgggatatc aaggaaatga tcattcaggc ctgtcagttc gataagcggg tgaaggaatt
    14461 catcggcccc gtatcacgat ttttctaccg ggtaattccc aatttctggt atcgccgcca
    14521 caaggggatg atgtccaact gggatgtttg agtgcggtat gccgtctttt ctttgccacc
    14581 ggggcggcca tggtaagtat gggcagcgtt tctttcatgt tttttcttga ctttttcggg
    14641 gcacacatga taggcgaaaa gaaaatttca agggaaaaat gaagcggcga tgatgaaggc
    14701 ggaaaaaatc ggcaggaatc tttgctggtg gtggcggact gataaagtct gcccggactg
    14761 agccgtttat cgaggtcgtg ggcagggaag gaccttccca tgacgaaacc ggaaatgaaa
    14821 gccatgggta tgacccatgg ctttttattt gccccgtggc gctggtgagc cgctacgggg
    14881 tttaaacaga cgatcattta caaaggagaa agcagcttat ggaacccatg aaggtattgc
    14941 tggaagatca agagattccg cggcagtggt acaatatcca ggcggacctg ccgacaccaa
    15001 tgcgtccgcc cctccacccg gccaccggaa agcccgtcca cccagatgat ctggcgccga
    15061 tctttcccat gaatctcatc gagcaggatg ctaccaccca gcgctggatc gacatcccgg
    15121 aagaagtcct ggaaaaatat ctcatctggc gcccctcgcc cctgtgccgg gcccggaatt
    15181 tcgagaaact tctcgacgcc ccggtgaaga tcttctacaa aaacgaaggt gccagcccgg
    15241 caggttccca caagcccaac accgccatcc cccaagccta ttacaacaag gccttcggga
    15301 tcaagcgcct ctccaccgag accggcgcag gacagtgggg aagcgccctc tccatggcct
    15361 gccagatgtt cggcctggaa tgtcgggtat tcatggtccg ggtcagttac gagcagaagc
    15421 cttaccggcg gatgatgatg aacgtctggg gggcggagtg catccccagt cccagcaaca
    15481 tcaccgaggc cgggcggaat gtcctggccg agcatcccga ttccccgggg agcctgggca
    15541 tcgccatcag cgaggccatt gaagacgcgg tgtcccatcc cggttcacgc tattccctgg
    15601 gcagcgtcct gaatcacgtc ctccttcacc agaccatcgt ggggctggag tcccagaagc
    15661 agttcgccaa acttggtctt tatcccgatg tcgtcatggg ctgcgccggc ggcggcagca
    15721 atttcgcagg gatatcgctg ccatatgtac gcgaccggat tcatgggaaa gatgtgagga
    15781 tcgtcggcgc cgaacccacc gcctgtccta ccctgacccg gggtcccttc gcctacgatt
    15841 tcggcgatct ggcgaaaaag acaccgcttc tgcccatgta caccctgggt cacgactacg
    15901 tgccggctcc catccatgcc ggcggcctcc gttaccacgg catggccccc atcgtgagcc
    15961 acctgctcaa agagaatctt atcgaggccc gggcctacca tcaactcgac acctttcaag
    16021 ccggcgtcat gtgggctcgg agcgagggat tcattcccgc gccggagacc aaccacgtca
    16081 tcgccgccgt ggttgatgag gcccggcgcg ccaaggagga ggggaaggaa aaggttatcc
    16141 tcttcaactg gagtggtcac gggatggtgg atctggcctc ctacgaggcc tatctgacgg
    16201 gcaaactcat cccccatgaa ctgccggatg aagagatcga ccgggccttg cgggctatcg
    16261 aggggttccc gaaaccataa ccattgcctt ccgaaacctc aggtcgctat gggatagaga
    16321 agcagcgggg gacggacgat cgtctggccc ccgctttttt tttgccgcat cccgccggta
    16381 atgattttta cagacagcgg ggcatttttg aggtaatgtc ggtttcaaca gaggcacggg
    16441 gcacgatacg acatgagtga gataacacgc tatgatgaca gggagatccg ctgcccaaag
    16501 ctcggacatg agcttacctt cgcctactgc cgccgggaag ggggggaact gccctgcttc
    16561 cgggtgatcc gctgctggga gggtttcttc cccgtggaga gctttctccg ggagagcctg
    16621 tccccggatg cctgggagac tttccgccgt cataccccgc cggacaagct caccagtctc
    16681 ctcgatctga ttgccaaggc acagcagcgg cggggaggtt aagggccggg gcgatagaaa
    16741 tagcggcaaa gatagcctga agatgaggtg caagaggcga tggtttttcc tcgacagacc
    16801 ctaagggacg gatggtgaaa caagagatga aaaagaggga attccagccc ctggcggacc
    16861 ggatgcggcc ccggcgtctg gccgaatatg ttggccagac gcaccttctg ggcaaggatg
    16921 gcctgctacg ccggtctata gaagaggacc gcctgttttc catgatcttc tggggaccgc
    16981 cggggtcggg gaagacaacc ctggcccggg tcatcgccca ggagacatcg tcacatttca
    17041 ccagcttttc cgctgtgttg tccggagtca aggagatccg tgccgtcgtc gaagcggcgg
    17101 tggccctgag ggatgagcag ggggaaaaga ccatcctgtt cgtggacgag atccatcgtt
    17161 tc
//
LOCUS       DAOSMF010000030        16858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_4071_length_16858_cov_22.883533, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000030 DAOSMF010000000
VERSION     DAOSMF010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 16858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16858
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4071_length_16858_cov_22.883533"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            186..2228
                     /gene="shc"
                     /locus_tag="PLT63_05010"
     CDS             186..2228
                     /gene="shc"
                     /locus_tag="PLT63_05010"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HOD98118.1"
                     /translation="MISSLGENNAASERSPLARAVAWLLRAQLPGGYWNAPVETNCCM
                     EAQWVLAMVFCGLDHPKKKGVVDYIVNEQRPDGSWDVYRHAEQGDINTTVECYAALRY
                     AGFSPDEEIISKARRWLLANNWTERIRVFTKYWLALFGEWPWAHTPDLPPEIIYLPKW
                     FPFNIHHFASWARATMMPLTIVSAEKPIRLLPPRMRLDELFPAGREAVDYRLAKTVNA
                     PFFSWPKFFLVTDKLLHAYNRLAKGSPLRRAAKQAALAWITAHQDEDGYWGGIQPPWI
                     YGIIAMQVMGLPQQQTNMARALNAPNLHWSEETPRGTRIKATESPVWDTMLAAGALLA
                     AGQTPGCRELDAAIDYLLAKENSHYGDWSQLMGKNTAPSGWAFERENRFYPDIDDTAV
                     CIVVLRKYRNLLDPHQDERRRKIDAAVERALNWLLAMRSQNGGWGAFDKDNTKAIITK
                     LPFCDFGEVLDPPSSDVTAHVVEALAACGYTQDHPVMRRAIRFLYDEQEEDGSWFGRW
                     GVNYIYGTWCVTTALFATGETAGTPRIARALAWLAGKQNDDGGWGESAASYMQPTLSG
                     RSGVSTASQTAWALLALANAGRRYENEIRAGVAFLVRTQTGDGTWIEKEFTGTGFPGY
                     GLGAKIDLRKGRPLPQGKELSRGFMLRYGYYCHYFPIMALSRAGELCKEITHNTEG"
     gene            2232..2492
                     /locus_tag="PLT63_05015"
     CDS             2232..2492
                     /locus_tag="PLT63_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98119.1"
                     /translation="MSLKNNYAIVGVGYTPQGVITGRTTLSFHLEACAAAIADAGLRR
                     EDVDGLICYRGFPPSAGETDVTPYLVAQHLGISPVFLAQDAN"
     gene            2508..3395
                     /locus_tag="PLT63_05020"
     CDS             2508..3395
                     /locus_tag="PLT63_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HOD98120.1"
                     /translation="MHAVGVLEAGLCSYVLLSYGHCGRSGDSMTRMLANLNAEDAVFG
                     HFGATGGYALAARRAMHEFHTGPETWKRIAVGQRKWANLNPRAMMYDRLMADADYDRS
                     PWVVEPFRLADNCLLTDGGRACILTTRERARDLRHTPVTIMGLGMCNPAADPAPARHL
                     AGPTGARWAGEMALHMAGITRDDIDACEIYDCFTYTVEITLQDYGFFSAGEGKDWLQG
                     GTIEPGGRMPVNTSGGLLSEAYFMGMTPLTEGVMQLMGRCGERQLGPKTGNKKPEIIM
                     CSDNGGILQTHSCYILTRG"
     gene            3402..3833
                     /locus_tag="PLT63_05025"
     CDS             3402..3833
                     /locus_tag="PLT63_05025"
                     /inference="COORDINATES: protein motif:HMM:NF013920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HOD98121.1"
                     /translation="MTKKRPLPKINADTKAFWEGCREHELRLQKCGDCGHLRHPPAFI
                     CPNCHSSRTVLVKASGRGRVYSYAVYHVAFDPAFQGDLPYVVALVALEEGPQIVTNIV
                     GCDPAAVSCEMAVEVVWDDRGKGVSLPIFRPINSHHNEVRK"
     gene            3830..4936
                     /locus_tag="PLT63_05030"
     CDS             3830..4936
                     /locus_tag="PLT63_05030"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOD98122.1"
                     /translation="MNDPRIVKLAELLVCYSVKIKPGDKVLVNGTTETAPLLKEVYAQ
                     VLRVGGHPLSLVSLPETEEIMYRYGTDQQLTFIHPPTKLGIETYDATISISGADNTKS
                     LSAVDPARVVIRNQARREIMDLFMQRSAAGELRWVGTLFPTNAYAQDAELSLRDFEDF
                     VFSACLPDLDDPIGYWERKSADQQRIVDWLRGRRRVRVTGPDTDLTLEVTDRLFINCD
                     GRHNMPDGEIFTGPVETSVNGTVRFSYPAIYQGRELTGVQLTFKEGKVTAARADKNEE
                     FLNKILDTDAGSRYVGEFAIGTNEGIRRFTKQILFDEKIAGSFHMALGAGYPETGSRN
                     RSAIHWDLICDLRRGGQIRVDDVLLYKDGRFTLG"
     gene            5153..7852
                     /gene="fdhF"
                     /locus_tag="PLT63_05035"
     CDS             5153..7852
                     /gene="fdhF"
                     /locus_tag="PLT63_05035"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:6221..6223,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HOD98123.1"
                     /translation="MEKKISLTIDGREVEAAAGQTILEVARENGIFIPTLCHYAGTTK
                     VGACRVCVVEVEGARSLVASCCMPVSPNMVIRTNTEKVRAAQRLIVELLWASGDHNCL
                     TCEQNGKCDLQDLVYWLEIESPRFNIKHPGYKHELSNAMISRDLNKCILCGRCIRACN
                     EIQVNEVLDFSRRGSRAKVGPAFDADYIDSECYFCGECADVCPVGAITFRQSRFRGRP
                     WEIQKVKTTCTYCGVGCQLDLNVHDGKIVKVTGNRDYVQPNGGSLCVKGRFGFDFVAS
                     PERLKKPLIRDKKGGELREASWNEALDVISGKFKAIKSKDGADSIAGLSSARCTNEEN
                     YLFQKFMRAVIGTNNVDHCARLUHSVTVAGLAAAFGSGAMTNSIDEIEYTDLIVATGT
                     NTTENHPIVGMKVKRAVRQHGTKLIVIDPRRIDLVKYADLWLRQKPGTDVAVFNGLMN
                     VMIAENLYDQEYVEKRTEGFAALKKIVEKYTPEYVEEISGVPAEDLRTAARMYGGAKR
                     ASILYAMGITQHITGTDNVKSVANLSMLGGNVGIEGGGVNPLRGQNNVQGACDMGALP
                     NVYPAYQQVAGDEARIKFEKSWGVKLSARPGLTIMEMMDGAHKGSIKGMYIMGENPMI
                     SDPDLAHVEASLRKLDFLVVQDIFLTETARLADVVLPSACFAEKDGTFTNTERRIQRI
                     REAVSPPGEAKSDWEIIAALAGRMGYAMNYSSAQEIMEEITRVTPSYSGITYKRLEKN
                     GLQWPCPTPDHKGTKYLHKDRFTRGLGLFHAIEYIPPAELPDESYPLLLSTGRVLYHY
                     HTGTMTRQSKGATERYPESLVEINPQDADRLGIFDGQMVKVTSRRGELQAKAKLTERS
                     AAGAIFMNFHFHEAAVNLLTNPVLDPIGKIPEYKVCAVRVEAA"
     gene            complement(8194..9426)
                     /locus_tag="PLT63_05040"
     CDS             complement(8194..9426)
                     /locus_tag="PLT63_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HOD98124.1"
                     /translation="MSNENLESKRWLIAGAAIVMQLCLGTVYAWSVFKKPLMTAHGWG
                     ETGVQATFMILIGVIGLAAAFGGTLVDKKGPRFVATIGGILFGIGTLAAGLADQLGSI
                     WLLYIGYGIIGGLGNGFGYVTPIATLIRWFPDKRGLVTGLAVMGFGAGAFFMGKIAPG
                     MVNSMGVANTFYIWGIIFLVAVTASAQLYKNPPKGWLPAGFKPAASSVSAADSFQFEE
                     AVKRPQWWMLWGMLFLNVSAGLGLISQLSPMAQDVIKKAQPMITPADLALAGGTILAI
                     ASIFNGLGRLIWAWTSDAIGRKMVFIIMFITQAILYVYLPQVTSQVLFTVLACYLLAC
                     YGGGFAVMPAFAADSFGPGYIGKVYGMMLTAWGAAGVAGPLIFAQVKDIALYVAAGML
                     IIGFVLALAYKPPARKTA"
     gene            9641..10150
                     /locus_tag="PLT63_05045"
     CDS             9641..10150
                     /locus_tag="PLT63_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98125.1"
                     /translation="MAEIKSTLDLIMERTKDLVLTEEEKRDIQTQERQARVRGWVQRY
                     RDGSLTGDDIKEYLANEGAAASEAAALLREECLTHVDPEEDNGKIFSLLQTALAINTA
                     PFEDLTADFHAEVQRLKIDSGHKALAVLYAQGINGSAVVPNPNLDQEWRRGLEMARKQ
                     FQKRLHRLK"
     gene            complement(10135..10782)
                     /locus_tag="PLT63_05050"
     CDS             complement(10135..10782)
                     /locus_tag="PLT63_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOD98126.1"
                     /translation="MKIFDGCRAFIWRHYQENNCNAYLIDRNKRIVIDPGHEHLFRHV
                     RQGLENASVSLAAVDLVVATHGHPDHLEGVRILGKPTLFAMNEEEYAYIRELAGGYFR
                     IPEPDFFLREGDLNVGSDSFQIISTPGHTPASICLYWPAAKALFTGDVIFNQGIGRTD
                     LPGGSGEQLKASIERLAELDVEFLLPGHGEPVIGKQAVEANFQAVRDYWFPYLSL"
     gene            complement(10893..13133)
                     /locus_tag="PLT63_05055"
     CDS             complement(10893..13133)
                     /locus_tag="PLT63_05055"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOD98127.1"
                     /translation="MNMLTVKVNPWRKLMFTWAILVMAVWSSPGYAETNVLNIRHWAA
                     PDHTRVVIDTSEEARYRVVKDDMRLSVEIDNAQYIKTRPRAIVIRKPGLEKIAVSTPT
                     ESLVLVDLYLAAHTETKVFRVKKFEGKPDRIVIDIILPEVARKEREDRERVKEYTKDK
                     VIVIDPGHGGEDPGAIGRHGTQEKKVVLEISKKVRDKLNAREGYYAFLTREGDYYVSF
                     KKRLRIAREYGADLFLSIHADAARSRQAYGSSVYVLSLRGASNEAAKILARNENLADI
                     VGGAGNGEGAKEESDPIILNMFQTQTMNSSKNFGNLLLKNMAAVNRVKFSTIQEAPFM
                     VLKLPEVPSILVETAYITNPIEEKKLRSRKFQNEIATTIADSAVEFLSSAPPRTPPIV
                     VARQDPDPPAPSQGTSGAEENAGKTSPPQTEGPPREAEPPKPEITGRGSVSSPKETAA
                     KNIPASPEEEPVSVRKAVKGTAKKSAPIFHMVKRGESVDRIARQYRVSPLLILKANNM
                     KPRDPLYVNRKIIIPTPAGGKTGRGKGQRDRAKRPVGDGGDAIRYYTVVKGDSLDSIA
                     ARHGTTYPILIKINGMKASDTLYAGKKIKVPAPEAPKDGGKQTGPDRTTPEKDTSSPG
                     PVKYRVKKGDTLEAIATRHGTTLTSLLRLNGMNVRDTLYAGKVIILREAAAGDAGGAK
                     KVGKRPERAKKATTVYIVKKGDSLEAIARRHKTTVGVLKDLNAGQRLSPLYVNQRLKI
                     PGASKM"
     gene            13302..14870
                     /locus_tag="PLT63_05060"
     CDS             13302..14870
                     /locus_tag="PLT63_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HOD98128.1"
                     /translation="MPDNRFKEALLNPAVFPVTWELVPGRGAREAAQEKVMTLAGQAA
                     SGGMIHALTLTDNPGGTPAMSADFMGSEIVRLGIEPLVHFTCKDKNRNAIESQLYALE
                     RAGIRNLLVMSGDYPVSGHQGRPAPVFDLDPVHVLQLISEMNRGLEYPGSKEVIRHHP
                     CDFFAGAVVSPFKATEAEQMAQYYKLKKKIAAGAQFIVTQLGYDARKFHEVLLFMKQK
                     GFNVPLVGNIYILPYGAAKMMNRNELPGSVVTDKLLTDIDRERSDPDKGVQARILRAA
                     RLYAILKGMGYSGVHIGGHNIKYEQVTEIIRQGEALATQWTSLIRHFDYPLPDGFYYY
                     ERDPETGLNLDKPAERRRRTCESGGEGAYLFARFMHRLLYEPGKNLYGAMKTVCGKVQ
                     GSSMEGIFHRLEHLVKVALFDCRDCGDCALIDAAYLCPMSQCPKNQRNGACGGSFQGW
                     CEVYPGKRRCIYVRAYDRLQSHGREGQLEEDIAPPCNWDLYQTSSWINYYLGKDHASK
                     KLGGKSSGNQATGT"
     gene            15016..15092
                     /locus_tag="PLT63_05065"
     tRNA            15016..15092
                     /locus_tag="PLT63_05065"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15050..15052,aa:Arg,seq:ccg)
     gene            complement(15212..16204)
                     /locus_tag="PLT63_05070"
     CDS             complement(15212..16204)
                     /locus_tag="PLT63_05070"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOD98129.1"
                     /translation="MLIGIVHTAGSPCGCAEALAGGLHALDHEALIVDSEEILLRAEE
                     LARRCDLVVDHTDTFLGWGVLRPFVRWELERFGARVAGTSARSCFLADDKIAAKGILN
                     AAGIPTPPGAAVNTAAMVLPPWLTPPLILKPACEHMSRGVVMVTTREEAREVMGALLL
                     RYRQPVLVETYIPGRELAISLLDGPEGLRVLPIIEWLTDWEDNNDVILTEAFKLRDNG
                     ADRGDMRRLLLPSDRLQELESLARKAFRSLGLRDYGRFDVRLAADGRCYFLEANTTPS
                     LENAEALAQSARWAGMDYPSLVAKILAAVRRRCDRERRQNRIGPVGGKRGLFSR"
     gene            16300..>16858
                     /locus_tag="PLT63_05075"
     CDS             16300..>16858
                     /locus_tag="PLT63_05075"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOD98130.1"
                     /translation="MSDRSTLREYLRGHMVVGDGAMASWLYQQGIPIGWCCEELNLSR
                     PELIRDIHRQYYKAGARLIETNTFGANRDALARHDLADSVYRINWKAAVLAREGVGPD
                     AWVMGSMTAVSARRIRHEVLPGHREIFIEQAEALLAGGVDGLILETFLDLEELLLALQ
                     TLRPRTDLPIIAQLACMEVGKTRDGH"
BASE COUNT         3609 a         5059 c         4911 g         3279 t
ORIGIN      
        1 ggaagaggcc atgaaagagg cctgccggtg cctccgctgc gacgcagagg tatgaccggg
       61 aagttcggct gttgatttaa accggagggg ggcaaggatt tgcggattgt tgcccccctt
      121 cattatgttc ccgcctgaaa agagatgtca tgtccccgcg tcaaataccc cgcgaaggaa
      181 atccggtgat ttcgtcactg ggagaaaaca acgccgcgtc ggaaaggtct ccgctggcgc
      241 gggccgttgc ctggcttctg cgggcgcagc ttccgggagg ttactggaac gccccggtgg
      301 aaacgaattg ctgcatggag gcccagtggg tcttggcaat ggtgttttgc ggcctcgacc
      361 acccgaagaa gaaaggcgtg gtggattaca tcgtcaacga gcagcggccc gacgggagct
      421 gggatgtgta tcgccacgcc gaacagggcg acatcaacac cacggttgaa tgctacgccg
      481 ccctccgtta tgcggggttt tcccccgacg aggagataat ctccaaggcc cgccggtggc
      541 tcctcgccaa caattggacc gagagaatca gagtcttcac caaatactgg ctcgcccttt
      601 tcggcgagtg gccgtgggcg cacacccccg atctgccccc ggaaatcatc tacctgccca
      661 agtggtttcc gttcaacatc catcacttcg cctcctgggc aagggcgact atgatgcccc
      721 ttaccatcgt ctcggcggaa aaaccgatcc gactccttcc cccgcgtatg cgtctcgacg
      781 aacttttccc cgccggccgg gaggccgtgg attaccgcct ggccaagact gtaaacgcgc
      841 cgttcttttc ctggcccaaa tttttcctgg ttacggataa attgctccat gcctacaacc
      901 gcctggccaa aggttccccc ctccgccgtg cggcaaagca agcagcgctg gcatggatca
      961 ccgcccacca ggacgaggat gggtactggg gcgggattca gccgccctgg atttacggga
     1021 tcatcgcaat gcaggtcatg ggtcttcccc agcagcagac aaatatggcc cgggccctca
     1081 acgcgccgaa tctccactgg tcggaggaaa ccccccgggg gacgcgcatc aaggcaacgg
     1141 aatcaccggt atgggatacg atgctggccg ccggggcgtt gcttgcagcc ggccaaaccc
     1201 ccggctgccg ggaactggac gcggcgatcg actacctgct cgccaaagag aattcccact
     1261 acggcgactg gtcgcaactg atgggcaaaa atacggcgcc cagcggctgg gcgttcgaga
     1321 gggaaaatcg gttctatccg gatatcgacg atacggcggt ctgtatcgtt gtcctcagga
     1381 agtaccgaaa cctgctggac ccccaccagg acgaacggcg ccggaagatc gatgccgcgg
     1441 tagaacgggc actgaattgg ctcctcgcca tgcgctccca aaacggcggc tggggcgcct
     1501 tcgacaagga caacactaag gcgatcatca ccaagctgcc gttctgcgac ttcggtgaag
     1561 tgctcgaccc gcccagctcc gatgtgaccg ctcacgtggt ggaagccctc gccgcatgcg
     1621 gatacaccca ggatcatccg gtgatgcgac gggcgatccg gttcctttac gacgagcagg
     1681 aggaggacgg ctcgtggttc ggccgctggg gcgtcaacta tatctacggc acctggtgcg
     1741 tgacgacggc gcttttcgcc acgggggaga cggccggaac cccccgcatt gcccgggcgc
     1801 tggcgtggct ggcgggcaag cagaacgacg acggcggctg gggggaaagc gccgcctcgt
     1861 atatgcagcc cacgctctcg ggccgctcgg gagtctcgac ggcatcgcag acggcctggg
     1921 cactgcttgc cctcgccaac gccggtagac gttacgaaaa cgaaatccgc gccggcgttg
     1981 cgtttctggt gcgcactcag accggcgacg ggacttggat cgaaaaggaa tttaccggca
     2041 ccggctttcc cggttacggc ctcggcgcaa aaattgactt gcgcaaaggc cgaccgctgc
     2101 cccagggcaa agaactgtcg cggggcttca tgctccgcta tggttactat tgccactatt
     2161 tcccgatcat ggccttaagc cgggccggag agctgtgcaa agaaataacg cacaatacgg
     2221 agggatgaca aatgagcctt aaaaacaatt atgccatcgt aggcgtgggc tacacccccc
     2281 agggtgtaat caccggccgc accaccctca gctttcatct ggaggcctgc gccgcggcca
     2341 ttgcagatgc cggtctgcgc cgggaggatg tggacggcct gatctgctat cgcggttttc
     2401 cgccctcggc gggggaaacg gatgtcacac cttacctggt ggcccagcac ctgggcattt
     2461 cgcccgtctt cctggcccaa gacgccaact gatcccggag tcaactcatg cacgcagttg
     2521 gcgtgctcga agcgggttta tgtagttatg tcctcctttc ctacggccat tgcggccgtt
     2581 ccggggacag catgacgcgg atgctcgcga atttgaatgc cgaggatgcc gtcttcggcc
     2641 acttcggcgc caccgggggc tacgccctgg cggcccgccg ggccatgcac gagttccata
     2701 ccggccccga gacgtggaaa cgtatcgccg tgggacagcg caaatgggcc aacctgaatc
     2761 cccgggccat gatgtacgac cgccttatgg ccgatgcgga ctatgaccgt tcgccctggg
     2821 tcgtcgagcc gttccgactg gccgacaatt gcctgctgac cgacggcggt cgggcctgca
     2881 tcctcaccac ccgggaacgg gcacgggacc tgcgtcatac gcccgtcacg attatgggcc
     2941 tggggatgtg caaccccgcc gccgaccccg ctccagcccg ccatctcgcc ggccccaccg
     3001 gggcccgctg ggcgggggaa atggccctcc atatggccgg gatcacccgc gacgatatcg
     3061 atgcctgtga aatctatgac tgcttcacct ataccgtcga gatcaccctt caggactacg
     3121 gattcttcag cgccggcgag gggaaggact ggctgcaggg ggggaccatc gagcccggcg
     3181 gccgcatgcc ggtcaacacc tcgggcgggc tgctctccga agcatacttc atgggaatga
     3241 ccccgttgac ggagggcgtc atgcagttga tgggccgctg cggggaacgg cagctcggtc
     3301 ccaagacggg caacaaaaag cccgagatca tcatgtgcag cgacaacggc ggaattctgc
     3361 aaacccattc atgctatatc ttgacgaggg gataggaaga gatgacaaag aaaagacctt
     3421 taccaaagat caacgcggat acaaaagcct tctgggaagg atgccgggaa cacgaactca
     3481 ggctccagaa atgcggagat tgcgggcatc tgcgccatcc gcccgctttt atctgcccca
     3541 actgccattc gagccggacc gtcttggtca aggcttccgg ccggggcagg gtctactcct
     3601 acgcggtcta tcatgtggcc ttcgatccgg ccttccaggg ggaccttccc tatgtcgtgg
     3661 ctttggtggc cctggaagaa ggaccccaga tcgttacgaa tatcgtgggg tgtgatcccg
     3721 cggcggtgtc ctgtgaaatg gctgtggagg tggtctggga tgaccgggga aaaggggtct
     3781 ccctccccat attccgtccg atcaattctc atcacaacga ggtaaggaaa tgaacgatcc
     3841 ccgtattgtc aaactggcgg aattgttggt atgttactcc gtaaagatca aacccggcga
     3901 caaggtcctc gtcaacggca ccaccgaaac ggcgcccctc ctcaaagagg tttacgccca
     3961 ggttctccgc gtggggggtc atcccctgtc cctggtgagc ctcccggaaa cggaagagat
     4021 catgtaccgc tacgggacgg accagcagtt gaccttcatc cacccgccta cgaagctggg
     4081 gatcgaaacc tacgatgcca cgatctccat ttccggcgcc gacaacacca aaagtctcag
     4141 cgcggtggat cccgctcgag tggtgatccg gaaccaggcc cgccgggaga tcatggacct
     4201 cttcatgcag cgttccgccg ccggtgaact ccgctgggtg ggcaccctct tccccaccaa
     4261 cgcctatgcc caggacgctg agcttagtct ccgggacttc gaggacttcg tcttctcggc
     4321 ctgcctgccc gatttggacg atcccatcgg ttactgggag cggaaaagcg ccgaccagca
     4381 gcggattgtg gactggttgc ggggaagacg gcgggtccgc gtcaccggtc cggataccga
     4441 cctgaccctg gaggtcaccg accgcctgtt catcaactgc gacggccggc acaacatgcc
     4501 cgacggggag atcttcaccg gtcccgtgga gacgagcgtc aacggcacgg tacggttttc
     4561 ctacccggcc atctaccagg gacgggaact gacgggggtg cagttgacct tcaaagaggg
     4621 aaaggttacc gccgcccggg cagacaagaa cgaagagttc ctcaacaaga tcctcgatac
     4681 cgacgcgggg tcccgctacg tgggcgagtt tgccatcggc accaacgaag gcatccggcg
     4741 cttcaccaag cagatccttt tcgacgaaaa gatcgccggc agtttccata tggcgttggg
     4801 cgccggctac ccggaaacgg ggagccgcaa tcggtcggcc atccactggg acctgatctg
     4861 cgatctccgc cgggggggac agatccgggt ggatgacgtc ctgctctaca aagacggccg
     4921 cttcaccctg ggttgaacca gtcgcgccgg acaaactttc ccatcaactc caccacaggt
     4981 caaaaggcac cggggattta ttgcctaaat acatatgtca ttaatgacat agttaaacga
     5041 tattttatcc ttgcaaaaaa agtcgattca gttcatattg cctcccggac ccggatcaga
     5101 cgggcccctc aattcgaaag gagtgaacaa tatatattaa aaggagcagg acatggaaaa
     5161 aaagatttcc ttaactatcg acggcaggga agtggaggcg gcggcgggac agacgatcct
     5221 ggaggttgcc agggaaaacg ggattttcat cccgacgctt tgtcattatg ctgggaccac
     5281 caaagtcggg gcctgtcgcg tctgcgtggt ggaggtcgag ggggcccgtt ccctcgtggc
     5341 ctcatgctgc atgcccgtca gcccaaacat ggtcatcagg actaacacgg agaaggtgcg
     5401 ggctgcccag cgcctcatcg tggagctcct ctgggcttcc ggcgaccaca actgcctgac
     5461 ctgtgaacag aacggcaaat gcgacctcca ggacctcgtt tactggttgg agatcgaaag
     5521 cccccgcttc aacatcaagc atcccggcta caaacatgaa ctgtccaatg cgatgatctc
     5581 ccgggacctg aacaagtgca tcctctgcgg ccgctgcatc cgggcctgca acgagatcca
     5641 ggtcaacgag gtcctcgatt tctcccggcg gggctcccgg gccaaggtcg gcccggcgtt
     5701 tgacgccgat tatatcgatt cggaatgcta cttctgcggc gagtgcgccg atgtctgccc
     5761 ggtgggggcc atcaccttcc gccaatcccg gtttcgcggc cgcccctggg aaatccagaa
     5821 ggttaagacg acctgcacct attgcggcgt cggctgccag ttggacctca acgtccatga
     5881 cggcaagatc gtgaaggtca cgggaaatcg tgattacgtc cagcctaacg ggggcagcct
     5941 ctgcgtcaag ggccgtttcg gcttcgattt cgtcgcttcg cccgagcggt tgaaaaagcc
     6001 gctcatccgc gacaagaagg gcggcgagct cagagaggca tcctggaacg aagcgctgga
     6061 tgtaatctcc ggcaagttca aagcaataaa aagcaaagac ggggcggaca gcatcgcggg
     6121 gctctcctcg gcacggtgca ccaacgagga aaattacctg tttcagaaat tcatgcgggc
     6181 ggtgatcggc accaacaatg tggaccactg cgcccggctc tgacactccg ttacggtggc
     6241 cggtctggcc gccgctttcg gcagtggcgc gatgacgaat tccatcgatg aaatcgaata
     6301 taccgatcta atcgtggcga caggtaccaa taccacagaa aaccacccca ttgtggggat
     6361 gaaggtcaag cgggccgtcc gccagcacgg gaccaaattg atcgtcatcg atccccggcg
     6421 gatcgatctg gtgaaatacg ccgacctctg gctccgccag aagccgggca cggatgttgc
     6481 cgtattcaac ggcctcatga acgtgatgat cgctgaaaac ctatatgacc aggaatatgt
     6541 ggagaaacgc accgagggct tcgcggccct gaagaagatc gtggagaaat acaccccgga
     6601 atatgtggag gaaatctccg gggtccccgc ggaagatctc cgcaccgccg cccggatgta
     6661 cggcggggcc aaacgggcct ccatcctcta cgccatggga atcacccaac acataaccgg
     6721 tacggacaac gtgaaatccg tcgccaacct gtccatgctc ggcggcaacg tagggatcga
     6781 gggcggcggc gtcaatcccc tccgggggca gaacaacgtc cagggggcct gtgacatggg
     6841 cgccctgccc aatgtctatc ccgcctacca gcaggtggcc ggcgacgaag cgcggataaa
     6901 attcgagaag tcctggggcg tcaagctctc ggcgcgaccc gggttgacca tcatggagat
     6961 gatggacggc gcccataagg ggtccatcaa agggatgtac atcatgggcg agaatcccat
     7021 gatctccgat cccgaccttg cccatgtgga ggcgtcgctc cggaaactcg acttcctggt
     7081 ggtccaggat atcttcctca cggagacggc ccggctggcc gatgtggtgc tgccctccgc
     7141 ctgtttcgcc gagaaggacg gtacctttac caatacggaa aggcggattc aacggatccg
     7201 cgaggccgtc tcccccccgg gagaggcgaa gagcgactgg gagataatcg ccgccctggc
     7261 cggccggatg ggctatgcca tgaattattc ctccgctcag gagatcatgg aggaaataac
     7321 cagggtcacc cccagttaca gcggcatcac ctacaagcgg ctggagaaga acggcctcca
     7381 gtggccctgc cccaccccgg accacaaggg gaccaaatac ctccataagg accgtttcac
     7441 ccggggtctc gggttgttcc atgccatcga gtacatcccg ccggcggaac ttcccgacga
     7501 aagctatcca ctgctccttt ctacgggacg ggttctctat cattaccata cggggaccat
     7561 gacccgtcag tccaaaggcg ccacggaacg gtatccggaa agcctggtgg aaatcaatcc
     7621 ccaggatgcg gaccggctcg gaatcttcga cggccagatg gtaaaagtca cgtcccggcg
     7681 gggagagttg caggccaagg cgaaactgac ggagcgctcc gccgccggcg cgatcttcat
     7741 gaactttcat tttcacgaag cggccgtcaa tctcctcacc aacccggttt tggaccccat
     7801 cggcaagatt cccgagtaca aggtctgtgc cgtccgggtg gaggcggcat aaccaagggg
     7861 agcagaaacc gagaggcgat gagcctcgtg tccacaatag cgattgcgtg atgtgaacag
     7921 ctttatgatg cccggttgat ttcctttact aggtaaatgc cgacggcagg atcatcctcg
     7981 ccggaatcat ctacatcctg cataaaaacg gcgaacatca ggcaatgaag gggcaagaat
     8041 tacagtagat gcatggacat atataaaccc agggaatatt tccatgccaa agggacgagt
     8101 gtttgcgaag caaaaaaacg ggcgggtcga tcaacccgcc cgtatcgacg gttgtgaact
     8161 tcagtttatt gctaccggcg ccgtcgcctt ggcctaggcc gtttttcttg ccggcggctt
     8221 gtaggccagc gccaggacaa aaccgataat gagcatcccc gccgccacat agagggcgat
     8281 gtccttgacc tgggcaaaga tcaagggtcc ggctacgccc gccgcgcccc acgccgtcag
     8341 catcatcccg tagaccttgc cgatgtagcc ggggccgaag gagtcggcgg caaaggccgg
     8401 catgacggca aagccgccgc cgtagcaggc cagcagatag caggccagaa ctgtgaagag
     8461 gacctgtgag gtcacctggg gcagatagac atagaggatc gcctgggtga tgaacatgat
     8521 gatgaacacc atcttccggc cgatggcgtc ggaggtccac gcccagatga gccgccccag
     8581 gccgttgaag atcgaggcga tcgctagaat cgtcccgccg gccagggcca gatccgccgg
     8641 ggtgatcatg ggctgggcct tcttgatgac atcctgggcc atgggggaaa gctgggaaat
     8701 aaggcccagt cccgccgaga cgttgaggaa cagcatgccc cagagcatcc accactgggg
     8761 acgtttcacc gcttcttcaa actggaagga atcggcggcg gaaacactgg aggccgccgg
     8821 cttgaaaccc gcggggagcc agcccttggg aggattcttg taaagctgcg cggaagccgt
     8881 gaccgcaacc aggaagatga ttccccagat gtagaaggta ttggccacgc ccatggagtt
     8941 gaccatcccg ggggcgatct tgcccatgaa aaaggcgccg gccccgaagc ccatgacggc
     9001 gagaccggta accaggcccc gcttgtcggg aaaccagcgg atcagggtgg caatcggggt
     9061 gacgtaacca aagccgttgc ccaggccacc gataatgccg tagccgatgt agaggagcca
     9121 gatgctgccg agttgatccg ccagcccggc agccagggtg ccgatgccga aaaggatacc
     9181 accgatagtg gccacgaacc ggggaccctt cttgtccacc agggtgccgc cgaaggccgc
     9241 cgccagaccg atgacgccga tgaggatcat gaaggttgcc tgaactcctg tttcgcccca
     9301 accgtgggcg gtcatcaagg gtttcttgaa gaccgaccag gcatagaccg tcccgagaca
     9361 gagctgcatg acgatcgccg cgccggcgat caaccacctt ttcgattcta aattttcatt
     9421 gctcataaaa cctcctaaaa gatttactta ggtgcatctt cctgtggccg gcacaagggg
     9481 caccgtccgc gccccggtcc aaaaatatgc ctgtgccgct gccaccgccg catgtagcaa
     9541 ataattctcg aaaaggcaat taaaagccct cccgtcattt ttgacatatt tataagtttc
     9601 tgctattaaa cgcagccgat atattcataa ggagaaccgc atggcagaga taaaaagcac
     9661 cctcgatctg atcatggaaa ggaccaagga cctcgtcctg acggaagaag agaaaaggga
     9721 catccagacg caagagcggc aggcccgggt caggggctgg gtgcagcgat accgtgacgg
     9781 gtcgctgacc ggcgatgaca tcaaggagta tctagcaaac gaaggggcgg cggcgtccga
     9841 ggcggccgcc ctgctccgcg aggaatgcct gacccatgtg gacccggagg aagacaacgg
     9901 gaagatcttc tcactcctgc aaacggccct ggccataaac accgctccat ttgaggacct
     9961 gacggcggat tttcatgcgg aggtgcaacg actgaagatc gattccggcc acaaggccct
    10021 ggccgtcctg tacgcccagg gaatcaacgg cagcgccgtc gttcccaacc caaatcttga
    10081 ccaggaatgg cgccgcggtc tggaaatggc gcggaaacag tttcagaaaa ggcttcacag
    10141 gctcaagtag ggaaaccagt agtccctgac ggcctgaaag ttggcctcca ccgcctgctt
    10201 gccgatcacc ggttccccgt gaccggggag gaggaattcc acgtccagct ccgcaagtct
    10261 ttcgatgctc gccttgagct gctcgccgct tccccccggc aggtccgtcc ggccgatccc
    10321 ctgattgaag atcacgtccc cggtaaacag ggccttcgcg gccggccagt acaggcagat
    10381 ggaagccggg gtgtggcccg gggtactgat gatctgaaat gaatccgacc cgacattcag
    10441 gtcaccttcc cgcagaaaga aatccggctc cgggatacgg aaataaccgc cggccagctc
    10501 acggatgtag gcgtactcct cctcgttcat ggcgaaaagc gtcggtttgc ctaatatccg
    10561 caccccttcg agatggtcgg ggtgaccgtg ggtggccaca accagatcca ccgccgccaa
    10621 agagacgctg gcattttcca gcccctgccg gacatgccgg aagaggtgtt catgccccgg
    10681 atcgatcacg atccgtttgt tccgatcgat taaataagcg ttacaattat tttcctgata
    10741 gtgcctccag atgaaggccc ggcagccatc gaatatcttc atccgatctt ccttcctgat
    10801 ttttaaacgg ccgccaaaac catggccggc cagagttcct tcccaaaaac ccgtccgttc
    10861 gacggccgca atcttatcac cgggggcgcc gttcacatct ttgaggcccc aggtatcttc
    10921 agacgttgat ttacataaag cggcgagaga cgctggcccg cattcagatc cttcaggacg
    10981 cccaccgtgg tcttgtgcct gcgggcgatc gcttcgagag agtccccttt cttcacgata
    11041 tagacggtgg tggccttttt cgctctttcc ggtcgctttc cgaccttttt tgctcccccc
    11101 gcatcgcccg cggcggcctc ccggaggata attaccttgc cggcataaag ggtatcccga
    11161 acgttcatac cgttcaatcg cagcagggaa gtcagggttg ttccatgtcg ggtggcgatc
    11221 gcctccaggg tgtctccttt tttcacccga tacttcaccg gaccgggaga tgatgtatcc
    11281 ttttcaggag tcgtccggtc cggacccgtc tgtttcccgc cgtccttcgg cgcctccggt
    11341 gcggggactt tgatcttctt ccccgcataa agggtgtctg aagccttcat cccgttgatc
    11401 ttaatcaata tcgggtaagt ggtcccgtgc cgggcggcga tgctgtcgag gctgtctcct
    11461 ttgacgacgg tatagtatct gatggcgtcc cctccatcac caaccggtct tttcgcccga
    11521 tccctttgtc ctttcccgcg gccggtcttg ccgccggccg gcgtgggaat gattattttc
    11581 cggttcacat agagggggtc ccggggcttc atattattag ctttaagtat aagcaggggg
    11641 gagacgcggt attgcctcgc tatccggtcc acgctttcgc ctcttttaac catatggaag
    11701 atgggggcgc tcttcttggc ggtccccttc acagccttcc gcaccgacac cggttcttcc
    11761 tcgggagacg ccgggatatt cttcgccgcg gtttccttcg gtgatgaaac tgatccccgc
    11821 ccggttattt cgggctttgg cggttccgct tccctggggg gaccctctgt ttgtggcggc
    11881 gacgtcttcc cggcgttctc ttccgccccg gaggtgcctt gagatggggc gggagggtcc
    11941 ggatcttgcc tggcaaccac gatcggcggg gtccgcggcg gggccgaaga gagaaactcc
    12001 acggcggaat cggcgatcgt cgtggcgatt tcgttctgga acttacggct ccggagtttc
    12061 ttttcctcaa tcggattggt gatgtaggcg gtctctacaa gtatggatgg gacctccgga
    12121 agcttgagaa ccataaacgg tgcctcctga atggtggaga acttcacccg gtttacggcg
    12181 gccatattct tcaggaggag attcccgaag tttttcgagg agttcatggt ctgggtctga
    12241 aacatgttca ggatgatggg atccgattct tccttggccc cttctccgtt tccggcaccg
    12301 ccgacgatat cggccaggtt ttcgtttctg gccaggattt tcgccgcttc gttgctggcc
    12361 ccacgcaggg ataggacata tacagaactg ccataggcct ggcggctccg ggcggcatcg
    12421 gcatgaatgc tgaggaagag atcagcgcca tattccctgg cgatcctcag gcgcttcttg
    12481 aaagagacat aataatcccc ttctcgggta agaaaggcat agtagccttc ccgggcgttg
    12541 agtttgtccc gcaccttttt gctgatttcc aggacgacct ttttttcctg ggtgccatgg
    12601 cggccgattg cccctggatc ttccccgccg tgaccggggt cgatgacgat gaccttgtcc
    12661 ttggtgtatt ccttgacccg ttctcggtct tcccgctctt ttcgtgcaac ctccggcagg
    12721 atgatatcaa tcacgatccg gtccggtttg ccttcgaatt ttttaactcg gaataccttg
    12781 gtttcggtat gagcggcaag atagagatcg accagcacca agctttccgt tggagtggag
    12841 acggcgatct tctcaagacc gggtttcctg atgacgatgg cccgcggcct ggtcttgatg
    12901 tattgggcgt tgtcgatctc gacgctgagg cgcatgtcat ccttgacaac gcgataccgg
    12961 gcctcttcac tcgtatcgat aacgacgcgg gtatgatccg gcgccgccca gtgtctgatg
    13021 tttaaaacat tcgtctcggc gtatcccggc gacgaccaga cggccataac cagtatcgcc
    13081 catgtgaaca tcagcttacg ccatggattt acctttaccg tcagcatgtt catgccacta
    13141 acaaatttcc ttggctactg caaggaaagg ttgcggacgg caggcaatac cggtgatttc
    13201 tcgtacgcaa ccttcatttt cccattaaat atggttgaca agcccaccgg taaatgcgat
    13261 tccagcgctc agccaccgac aagggaaaaa acgaactgac aatgccggat aatcgtttca
    13321 aagaagcttt gttgaacccc gccgtcttcc ccgtcacctg ggaactggtg ccgggccggg
    13381 gcgccaggga agcggcccag gagaaggtca tgaccctcgc cggccaggcg gcttccgggg
    13441 ggatgatcca tgccctgacc ctgaccgaca atccgggcgg aaccccggcc atgtccgcag
    13501 attttatggg cagtgaaatc gtccgcctcg gcatcgagcc gctcgtccac ttcacatgta
    13561 aagacaaaaa ccgcaatgcc atcgaaagcc agctttacgc cctggagcgg gctgggatcc
    13621 ggaatctcct ggtaatgagc ggcgattatc cagtatcggg acatcaggga agaccagcac
    13681 cggtcttcga tctggacccg gttcatgtcc tgcagttgat ctcggaaatg aaccgggggc
    13741 tggagtatcc gggaagcaaa gaggtcatcc ggcaccatcc ctgtgatttc ttcgccggcg
    13801 ccgtcgtttc gcctttcaag gcgaccgagg cggagcagat ggcccaatat tacaaactga
    13861 agaagaagat cgccgcggga gcgcaattca ttgttaccca actgggatac gacgcgagaa
    13921 agtttcatga agtgttgctg ttcatgaaac aaaagggctt caacgttccg ctggtgggca
    13981 acatctacat cctgccgtac ggcgccgcca agatgatgaa ccgcaacgag ctgcccggct
    14041 ccgtcgtgac ggacaagctc ctcacggaca ttgaccggga gcggagtgat ccggacaaag
    14101 gagttcaggc gaggatcttg cgcgccgccc ggctgtatgc catcctcaag ggcatgggtt
    14161 acagtggggt tcacataggc gggcacaaca tcaaatatga gcaggttacg gagatcatcc
    14221 ggcaggggga agcccttgcc acccaatgga ccagtcttat caggcacttc gattatcccc
    14281 ttccggacgg tttttattac tatgagcgcg atccggaaac gggcctcaac ctggataagc
    14341 cggccgaaag acggcgccgg acctgtgaat ccggcggcga aggagcctat ctttttgcgc
    14401 gcttcatgca tcgactgctg tacgagccgg gaaagaatct ctacggcgcc atgaagacag
    14461 tctgcggtaa ggtgcaggga agcagcatgg aagggatttt tcaccggctg gagcacctgg
    14521 tcaaggtcgc ccttttcgac tgccgcgact gcggggattg cgccctcatt gacgcagcct
    14581 acctatgccc catgtcccaa tgcccgaaaa accagcgcaa cggggcctgc ggcggcagtt
    14641 tccagggttg gtgcgaggtt tatcccggaa agaggcggtg catttacgtc cgggcctacg
    14701 accgcttgca gagtcacggc cgggaaggac aactggagga ggacatcgcc cccccctgca
    14761 actgggatct ttatcaaacc tcatcctgga tcaattacta tctcgggaag gatcacgcct
    14821 ccaaaaagct gggtggcaaa tcctccggaa atcaagcgac gggcacataa atattttccc
    14881 catccattcc tttcctgccc tcgccccgga aacagaaacc acggagcaag aacttgggac
    14941 tccgaggagt cacgaggata acagcgaatt tatcgattga agaatctgaa agaatatagt
    15001 agaaggtccc ggcatgggcg attagctcag gtggatagag cgttggtctc cggaaccaaa
    15061 ggccccgcgt tcgagtcgcg gatcgcccac caaatgaaat caaggggtta gattgttgat
    15121 ctaacccctt tagcctttct gcgctaatct gcatgctacc cataaagagc gggatgggac
    15181 aattccaccg agctggaatg taaaagaaca atcaacggct aaataatcct cttttccccc
    15241 caaccggacc gattcggttt tgccgccgtt ccctgtcgca tcgccgtcgg accgccgcca
    15301 ggatcttggc taccagggat ggataatcca ttcccgccca ccgggcggac tgggccaggg
    15361 cctcggcgtt ttccaggctg ggagtcgtgt tggcctctaa aaaatagcag cgaccgtcgg
    15421 ccgccaggcg gacgtcaaag cggccgtaat cccgtaatcc caaagagcga aaggccttcc
    15481 gggccaggga ttccagttcc tgcaaccggt ccgaaggaag aagaagcctc cgcatatccc
    15541 ccctgtccgc accgttgtcc ctaagcttga aggcctccgt aaggataacg tcattgttgt
    15601 cctcccaatc ggtaagccac tcgatgatcg ggagcactcg gagtccctcc ggaccatcca
    15661 gaagcgatat ggccaattct cggccgggaa tgtaggtttc aaccaggacc ggctggcggt
    15721 accggagcag gagtgccccc atgacctcac gtgcctcttc ccgggtggtt accattacca
    15781 cgccccgact catatgctca caagccggct tcaggatcag gggcggtgtc agccaggggg
    15841 gaagtaccat ggcggcggta ttaacggctg ccccgggagg ggtggggatc ccggcggcat
    15901 tcaggatccc cttggcggca atcttgtcgt ccgccagaaa acacgaccgg gcggaggttc
    15961 cggccacccg ggcgccaaag cgttccaact cccatcggac aaaggggcgt aaaacccccc
    16021 atcctaaaaa ggtatcagtg tgatccacga ccaggtcgca ccgccgggcc agttcttccg
    16081 cccgcaataa aatctcctcg gaatcgacaa taagggcttc atggtccagg gcgtggagac
    16141 cccccgccaa ggcctcggcg caaccacagg gcgaaccggc ggtatggaca atccctatga
    16201 gcacggcaac ttctccttct tccttacata acccttgaca acccctgatt gcccctctta
    16261 tactttaaat cgacttaaat ccatcggaaa tcgaggaaga tgtcagatag aagcactctc
    16321 cgggagtatc tccgggggca catggtagtc ggcgatgggg cgatggcctc ctggctgtac
    16381 cagcaaggga ttcccatcgg ctggtgctgc gaggaattga atctctcgcg tccggagctc
    16441 atcagggaca tccaccggca atattacaaa gctggtgccc gcctcatcga gaccaacacc
    16501 ttcggcgcta accgcgacgc cctggcgcgc cacgatctgg ccgacagcgt ttaccgcatc
    16561 aattggaaag cggccgtcct ggcccgggaa ggggtcggac cggatgcctg ggtcatgggc
    16621 agcatgaccg ccgtcagtgc ccggcgcatc cgtcacgaag tcttgccggg gcaccgggag
    16681 atattcatcg agcaggccga ggcgctgctc gccggcggcg tggacggcct tatcctggag
    16741 accttcctcg acctggagga actgctcctg gccttgcaga ccctgcgtcc ccgcactgat
    16801 ttaccgatca tcgcccaact ggcctgcatg gaggtgggaa agacccggga cggccatt
//
LOCUS       DAOSMF010000031        15795 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_4424_length_15795_cov_20.795807, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000031 DAOSMF010000000
VERSION     DAOSMF010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 15795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15795
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4424_length_15795_cov_20.795807"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            476..721
                     /locus_tag="PLT63_05080"
     CDS             476..721
                     /locus_tag="PLT63_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98131.1"
                     /translation="MPRGDGTGPAGKGPGTGQGRGQGKGQGRGQGQGLGRMGGPLAGG
                     AVGFCVCPNCGQRQSHERGIPCYQKQCPNCGTAMIRE"
     gene            727..942
                     /locus_tag="PLT63_05085"
     CDS             727..942
                     /locus_tag="PLT63_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98132.1"
                     /translation="MSAYTFKHVYGPVPSRRLGRSLGVDLVPFKTCTYDCIYCHLGRT
                     TNKTVERKEYVEIDAVGADQQTHLCDH"
     gene            984..1550
                     /locus_tag="PLT63_05090"
     CDS             984..1550
                     /locus_tag="PLT63_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98133.1"
                     /translation="MGAAVSSHVAPYTGFCFTDAIGTPLANATLSGAARIVISRGRNM
                     AIALAASQDHIATVFDSADSFVVIESGVVRQRRVEAIPAGLTMMQMRQKLMELSVNVL
                     LCGAIATSTRQFIEGAGIVVIPFLKGNLTDVETGFFSNHLDDPAFYLPGCRRGWVGLG
                     MGPCGRGTGRGRRGAAGNTKGQRRRGIS"
     gene            1547..1918
                     /locus_tag="PLT63_05095"
     CDS             1547..1918
                     /locus_tag="PLT63_05095"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOD98134.1"
                     /translation="MKIAVTAQKPGWTEPLDSRFGRASWFVIIDTETKKWEAAENLQN
                     RQSAQGAGIQAARTIIDRGVEALISGNVGPKAFKVLQAAGVKMYRAESGRQQTVADAF
                     VAWERKDLEEISSATVDGHSI"
     gene            1949..2299
                     /locus_tag="PLT63_05100"
     CDS             1949..2299
                     /locus_tag="PLT63_05100"
                     /inference="COORDINATES: protein motif:HMM:NF036294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HOD98135.1"
                     /translation="MPKGDRTGPAGMGPMSGRGFGYCAGYAMPGYAGRGPVRGAEWFS
                     GFLRYGRGNRSRFHRPGFFGRQSVGAYDYYAPASGPIPSKEMEIQALKDELQDYERVT
                     QQLRSRLTELEKNP"
     gene            2296..3168
                     /locus_tag="PLT63_05105"
     CDS             2296..3168
                     /locus_tag="PLT63_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD98136.1"
                     /translation="MKVAFASGKGGTGKTTVAVNIANILQQTGVDVQLLDCDVEEPNA
                     HLFLLPRIDGSIPATVRIPEVAEEHCTGCGVCAEFCQYNAITVFKNTVIVFPELCHGC
                     GVCAEFCPERIITESDRAIGVIEGGAAGGIGFLQGRLNVGEVLAPPLIRQVKEKVPAA
                     AVTLVDCPPGTSCPVIEAVRDMDLVVLVSEPTPFGFYDLTLMAATLKTLAIPSGVVVN
                     RCDIGNDELYLFCRREGLPILAEIPHDRRIAECYSRGALITEALPEYRELFAGLWKQV
                     LTLLPSRPDREGRE"
     gene            3165..4070
                     /locus_tag="PLT63_05110"
     CDS             3165..4070
                     /locus_tag="PLT63_05110"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD98137.1"
                     /translation="MNRGASMTKEITILSGKGGSGKTTVTGALAALEKNAVLVDADVD
                     APDLHLLLQPRIRRSTIFYGSKKAKTIPADCIHCGLCVGACRFDAIAPGEPPEICIIF
                     CEGCGMCYRICPVEAIEFAEARTGEWYVSDTAYGPMVHARLDPAQGNSGKLVSLVREE
                     GRKIAAANQAEFILTDGPPGIGCPVIASLAGTGFVLIVTEPTLAGEHDLRRLAELLAL
                     FKIPAGLCLNKWDLNPEMAARMEAAGRDLGMHAVGRIRFDLTTVEAQLQGRTVMDYPA
                     SPAAADIEALWKNVLNLLRKNDSGG"
     gene            4078..4962
                     /locus_tag="PLT63_05115"
     CDS             4078..4962
                     /locus_tag="PLT63_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOD98138.1"
                     /translation="MSNTEDALPLYGKGQKQQQENRDRTEKHQQEEKQLRERMAQIRH
                     KILVLSGKGGVGKSTIAVNIAVGLGMEGKKVGLLDVDFHGPSIPTLLRLGNQTLRTDG
                     KNAFPLDYVYGIKVVSLGLLLPDQDEAVIWRGPLKMGVIRQFLADIQWGDLDYLIIDF
                     PPGTGDEPLSVAQMLPESDGAVIVTTPQEVSLADVRKSISFCRQVKVPVLGVVENMSG
                     LNCPHCGKLIELFKTGGGESMAKKLKVPFLGRIPLEPAVVEASDSGLPFIYHYNQTGA
                     AQAFREGIIKTILALDDR"
     gene            5063..5410
                     /locus_tag="PLT63_05120"
     CDS             5063..5410
                     /locus_tag="PLT63_05120"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOD98139.1"
                     /translation="MQLMRIAVPTVDGYLCPHFGHCGAFVIVDVDKDERKILKTEIHE
                     APPHEPGLLPRWLAEKGADLIIAGGMGARAQDLFNQRGIGVVVGAPSETPEKIVGDYL
                     QGALFCGQNLCDH"
     gene            5678..6604
                     /locus_tag="PLT63_05125"
     CDS             5678..6604
                     /locus_tag="PLT63_05125"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOD98140.1"
                     /translation="MSSGKSAPEPSANRREIDGETFQAMIMKIRRRPRQQISGHWFNI
                     VEAAGHRFLQGPLNTDVRGQRRNGGLAYITRLVRTEDIAGFWPETTGRLLAPEECHAA
                     AAFISSLDGRDVELIVPLRNRALPVRWERFEDSREYPLYAAHIHPRILSALSEITFQR
                     LLDVGCGSGNLLAAIRRDNPHAYYAGFDIGPDNVEAAKEKGLENIREEKGENVATVYA
                     MAPPFDVIIFCGILNRQIMDGDSARTILKKSLTRLAKGGLVIITGYSSCHFCARELKK
                     KGLTVLNKSIPENIFRDYDEFYLRQFYVARKD"
     gene            6758..7105
                     /locus_tag="PLT63_05130"
     CDS             6758..7105
                     /locus_tag="PLT63_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HOD98141.1"
                     /translation="MKKVALFAFNGEAMCLVHVLLNALEMKDKGYEVKVIIEGTATKL
                     IRDLTAAPTPFTPLYEKVKAAGLIDCVCQACSKKTGSYDEATAQGLPVCGEMMGHPSM
                     ARYREAGYELIVF"
     gene            7192..7767
                     /gene="tsaA"
                     /locus_tag="PLT63_05135"
     CDS             7192..7767
                     /gene="tsaA"
                     /locus_tag="PLT63_05135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HOD98142.1"
                     /translation="MRKTRGKGVEAMPAMPASGTDCPATGAAVIYHPIGIIHSPFSDW
                     RGMPIQPAGARGVKGRVEIAAPYRQALKDLESFSHIILLYHFHLSKDYHLEVIPFLDT
                     VPRGLFATRAPRRPNPLGLSIVKLTGIEGGVLHIEDVDIVTGTPLLDIKPYVPAFDIV
                     TEATGGWTQGKEKEVEVMRSDGRFHAASEPR"
     gene            7883..8908
                     /locus_tag="PLT63_05140"
     CDS             7883..8908
                     /locus_tag="PLT63_05140"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOD98143.1"
                     /translation="MFHEERCDFCGDCLARCHYLDFDNKTGGEAFEKLVRGEKSDWIY
                     DCVTCIACNEYCPKDARPFDLILRRMEEAGDFTDPKLKAQMADRFKAEGEPRPMEPKD
                     RVMSICVMDGSMPWAIQGSLFQELPILKGRHYFCNVLFAHMGDESIMRDRLQGLVNNL
                     AKSRAKEIVFVHDDCYALLAGMAPEFGIKLPFRPISILEYLAEYLKNHPDRITRLNMK
                     VAYQRPCASRYTPEKDHFLAEIFSLIGVERVARKYDGINAICCGVELAGPNLKLFPRG
                     KNFAPFQEKNIQDAKDSGAEAIVYLCPMCFRTLNKKAREAGMKNYMISDICRLALGET
                     LPEDKPA"
     gene            9165..9563
                     /locus_tag="PLT63_05145"
     CDS             9165..9563
                     /locus_tag="PLT63_05145"
                     /inference="COORDINATES: protein motif:HMM:NF013738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HOD98144.1"
                     /translation="MEDIGNEILSPQLIDHARQPRNKGILPGYNAEGQITGPCGDTMA
                     FWLEVKDGTVKNASFYTTGCGTSLACGSIATCLVRGMRVAAAAALSQEDILNALGGLP
                     PEAEHCALLAANTMRAACENYLKDSRRAEK"
     gene            9684..10643
                     /locus_tag="PLT63_05150"
     CDS             9684..10643
                     /locus_tag="PLT63_05150"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOD98145.1"
                     /translation="MAAPSLKPVDNIEILSLQDNYIEMTATDNSAVVTRAIPLKDGCF
                     RLSVLAEHGFAAVVKTGVNGKTHTLLFDFGFSEGGAAYNAKILGVDLGAVEAVALSHG
                     HNDHTGGFDQLAALLGRREIPFFVHPQVFRKPRYLKIPVRDMKIHFSPLTREQIAATG
                     LKLIETSEPVTFLDDTVLFLGEVPHRTDFEKGFPIARYQEDGQEKWDPIEDDTSVVMH
                     LKDKGLVVLSGCAHAGIVNTVNYAREITGVDKVHAVMGGFHLSGIYFEGIIDRTVEEM
                     KKIDPDYVIPCHCTGRNAIMAFERAMPEKFILNMAGTKLTFGA"
     gene            complement(10947..12026)
                     /gene="cobD"
                     /locus_tag="PLT63_05155"
     CDS             complement(10947..12026)
                     /gene="cobD"
                     /locus_tag="PLT63_05155"
                     /EC_number="4.1.1.81"
                     /inference="COORDINATES: protein motif:HMM:TIGR01140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-phosphate decarboxylase CobD"
                     /protein_id="HOD98146.1"
                     /translation="MPRSQFQHGGDIRRLAARAGVSQDELLDFSANINPLGLPVGFRS
                     LIRRRLNEILHYPDPRAAGVIAAVSRRYGIPPEEIIVGNGSTEILYALPRALSRGRAV
                     IPVPSYADYAVASALAGMEVVKVALSEHRGFILEMDELEDHLQGDEIVFIGRPNNPTG
                     LSCAAVAIRAAARRHPDTVFVIDEAFADFVPAMDRLLVRRPPNVIVLCSLTKFHAIPG
                     LRLGFAAGAAAVMEKIGGFILPWTVNALAQAAGETVLDDDDYRRRTLALIEKERRFLS
                     EGLRDRFAFHVYPGEANFLLARIDGRKMDAPGLAAKLLKKGIAIRRCDNFTGLDRRFF
                     RVAVRKRPENEALLAALQDIWTALD"
     gene            12726..14111
                     /gene="rmuC"
                     /locus_tag="PLT63_05160"
     CDS             12726..14111
                     /gene="rmuC"
                     /locus_tag="PLT63_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOD98147.1"
                     /translation="MPDAHLIATSSIMAGNPRGKSSMTDVFFYALLAADGLFLVLLVA
                     LLFKSSSRPLAAIDHRLEGMETLQERLERTLREDGPRDREELNRSLRDVSESLLVRLA
                     ENMSLQKDQWEIFSRQLTDLAVQSIQKLDRINESVALQLRNLQEDQRRQLEAIRATVD
                     EKLQDTLEKRLGESFRLVSERLETVQKGLGEMQTLAAGVGDLKKVLTNIKTRGTFGEI
                     RLGTLLEEILSPNQYAVNVATKPDSDHRVEFAVRMPGRHDDPRQVVWLPLDAKFPQED
                     HLRLLEAQEQGNPGQVEEASRQLERTLRDMAKSIRDKYLAPPHTTDFAIMFLPTESLY
                     AEVLRRDGLFAQLQREFRVVITGPTTLAALLNSLQMGFRTLAIERRSSEVWELLGAVK
                     TEFANFGGILEKTRKKIMEAGNTIEKAAVRSRAIERRLRDVQGLPAPEDGGGESGAED
                     AGDNGAPYNRS"
     gene            14119..14916
                     /locus_tag="PLT63_05165"
     CDS             14119..14916
                     /locus_tag="PLT63_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HOD98148.1"
                     /translation="MAGPMVVPAFAAAAPGAAVPAGSSKRRGEETALAGERSFLLKGT
                     IVNTGAVIAGSLIGLAAGKRMPPGLKTILMQCLGLSTILIGLQMALSERDVIPAIGCL
                     LLGALTGELLKIESGIDRLGEWLKARARSESSTFVEGFVTASLLYVTGAMVIVGSIQD
                     GATGDATTLYIKSLLDGVASIAFASTLGIGVAFSALSVLCVQGVLTLLAAHLSFLQQP
                     AVLGAITATGGLLIVAIGLNLLNVAKIRIGNLIPALIYAAIWAVYGG"
     gene            complement(15009..15551)
                     /locus_tag="PLT63_05170"
     CDS             complement(15009..15551)
                     /locus_tag="PLT63_05170"
                     /inference="COORDINATES: protein motif:HMM:NF022766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3124 domain-containing protein"
                     /protein_id="HOD98149.1"
                     /translation="MNNMGRLIRTIAIIALFFSVIACSGKEAPPLNGQAAGDFRPETL
                     KVADTAENKLRGQVLYVPIYSNIPYIESKKFDLSAFLAIHNTDLKNPIKVTKVLYFNN
                     DGKLVKEFLVGEHPLPPLGAVNYFIPQSDTSGTGANFLVEWLSDVPVSEPLIESIMLN
                     LEGNRGISFLSRGKVIREMR"
     gene            complement(15683..>15795)
                     /locus_tag="PLT63_05175"
     CDS             complement(15683..>15795)
                     /locus_tag="PLT63_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOD98150.1"
                     /translation="TGRNPQTGKAIKIKAKKVPKFTAGKKLRDAVAAAKK"
BASE COUNT         3497 a         4557 c         4693 g         3048 t
ORIGIN      
        1 ttccgccgga tcaaggccct ggggatcaag gagccccgac cgggaagatc ctgacaacag
       61 cggaccgaca atccgtcttc cggagaatcg gcgcggcggc gcagcggtcc ctccgtcggt
      121 ttttaccagc gagcatgcag tcgaccgcgg ttatgacctg taataagagc ggcggcaata
      181 cgatggaagg ggctttgcaa tacatcagct gataaccgaa gcaattttgc gatgccagtc
      241 cttgcataga acgtccattc cgcacccatc tgtttgactt catgaaatat tttccgtctc
      301 actgctgaac ggcacaagct ttgctgcatt aatgatcgtc tcggccgcaa tcaaccgcaa
      361 aacggatatc gctgccgata caaggcacta tcgctttcag ccgacacgga tcaaaggaaa
      421 gatcaatact gtcttgtggc ttgcatacaa ctaataacat gggggaggaa cgacaatgcc
      481 cagaggagac ggaacgggac cggccggcaa gggaccgggg acgggacagg gaagaggtca
      541 gggaaaagga caaggccgag gacaaggtca gggattggga cgcatgggcg gtcccctggc
      601 cggtggggcg gtgggctttt gcgtctgccc caattgcggg cagcggcagt cccatgaacg
      661 gggcatcccc tgttatcaga agcagtgccc gaactgcggg acagcgatga tcagagaata
      721 gggacaatgt ccgcctacac cttcaagcat gtatatggcc ccgtaccttc gcggcgactg
      781 ggaagatccc tgggtgtcga tctcgtcccc ttcaagacct gcacctacga ttgcatctat
      841 tgccacctcg gacgcacgac caacaagacc gtggaacgaa aggaatatgt ggaaatagat
      901 gcagttggcg ccgaccaaca aacgcatctt tgcgaccatt gaccgtccct gtagtcgcac
      961 aatcactacc ataaggaatg gatatggggg cggcggtcag ctcacatgtc gccccttaca
     1021 ccggcttttg ctttacggac gccatcggca caccccttgc aaacgctaca ttgtcaggag
     1081 cagcaaggat cgttatttca cggggcagga atatggccat cgcgctggca gcatcacagg
     1141 atcatatcgc cacggttttc gattccgccg attctttcgt cgtcatcgaa tccggcgttg
     1201 tccggcagcg gcgcgtcgaa gccatcccgg cgggactgac catgatgcag atgcggcaaa
     1261 agctgatgga attgagcgtg aacgttctcc tctgcggggc tattgccact tccacccggc
     1321 aattcatcga gggggcgggg attgtcgtaa tccctttcct gaagggaaat cttaccgacg
     1381 tggaaaccgg atttttctcc aaccacctcg atgatccggc tttttacctg cccggatgcc
     1441 gccggggatg ggtgggtctc ggtatgggtc catgcggccg cggaacggga agaggccgac
     1501 gcggggcggc gggcaacaca aaaggacagc gaaggagagg aatatcatga agatagcggt
     1561 aacggcgcaa aaacccggct ggacggaacc attggacagc cgtttcggac gggcctcctg
     1621 gtttgtaatt atcgatacgg agacgaaaaa gtgggaggcc gcggagaacc ttcagaaccg
     1681 ccaatcggcc caaggtgcgg gcatccaggc cgcacggacc atcattgacc ggggagtgga
     1741 ggccctgatt tccggcaatg taggtccgaa ggcctttaag gtactccagg cggcgggggt
     1801 aaagatgtac cgcgccgaat ccggccggca gcagacggtg gccgacgcct tcgtcgcctg
     1861 ggaacggaaa gatttagagg aaatcagctc ggccaccgtg gatggtcaca gtatatgact
     1921 aataaatttt attaaggaga tactgcatat gcctaaagga gatcgcacgg gcccggcggg
     1981 aatgggtccc atgagcggca ggggcttcgg ttactgcgcc ggatatgcca tgcccggtta
     2041 tgccggcagg gggccggtta gaggtgcaga atggttttca ggtttcttga ggtatggtcg
     2101 gggcaaccgg agcaggttcc atcggcccgg atttttcggc cgacagtcgg tcggggccta
     2161 tgattattat gccccggctt ccggaccgat cccgagcaag gaaatggaaa tacaggctct
     2221 caaggacgag ttgcaggact acgaaagggt aacgcaacag ttgcgttccc gtctgacgga
     2281 actggaaaag aacccgtgaa ggttgctttt gccagcggca agggcggtac gggcaagacc
     2341 accgtagccg tcaatatcgc gaatatcctc cagcagaccg gcgtggacgt ccaactcctc
     2401 gactgcgatg ttgaagaacc aaacgcccat ctctttctcc tgccgcggat cgacgggagc
     2461 attccggcga ccgtgagaat tcctgaggtc gcggaagaac attgcaccgg atgcggcgtc
     2521 tgcgccgagt tctgccaata taacgccatc acggttttca agaataccgt gattgtattt
     2581 ccggaactgt gccacggatg cggcgtctgc gccgagttct gtcccgaacg gatcatcacg
     2641 gaatccgacc gggccatcgg ggtaatcgaa ggaggcgccg ccggggggat aggtttctta
     2701 cagggacgcc tcaatgtggg cgaggtgttg gccccgcctc tcatacgcca ggtaaaggag
     2761 aaggttccgg cggcggccgt cacccttgtc gattgtcccc cgggaacatc ctgccccgta
     2821 attgaagcgg tgcgggatat ggacctggtg gtgcttgttt ccgaaccaac tcccttcggt
     2881 ttttacgacc tgacgctgat ggcggcaacg ctgaaaaccc tggccattcc cagcggggtc
     2941 gtcgtcaatc gctgtgatat cggcaacgac gagctgtatc tattctgccg ccgggaaggc
     3001 ctccccatcc tcgccgagat cccccatgat cggcggatcg cggagtgtta ttcccgcggc
     3061 gccctgatta ccgaggcact gccggagtac agagagctgt ttgccggtct ttggaaacag
     3121 gtattgacat tgctgccctc ccggccggac cgggaaggac gagaatgaac aggggcgcct
     3181 ccatgaccaa ggaaatcacc attctcagcg gcaaaggtgg ctcaggaaag acaaccgtta
     3241 ccggcgccct ggctgccctg gaaaagaatg ccgtcctcgt agatgccgac gtggacgccc
     3301 cggatcttca cctccttctc cagccacgga tcaggaggag cacgatcttt tacggatcca
     3361 aaaaggcgaa gacaatcccg gcggattgca tccattgcgg cctctgcgtc ggcgcctgcc
     3421 gttttgacgc cattgccccg ggcgagcctc cggagatctg cattattttt tgcgagggat
     3481 gcggcatgtg ctaccggata tgtcccgtgg aggccatcga gtttgcggaa gcccgcaccg
     3541 gggagtggta tgtgtcggat accgcctatg gccccatggt gcatgcccgt ctggaccccg
     3601 ctcagggtaa ttcggggaag ctggtcagcc tggtccgcga ggaaggacga aaaatcgccg
     3661 cggcgaatca ggccgaattt atccttaccg acggtcctcc gggaatcggt tgtcccgtaa
     3721 tcgcctcgct ggccgggact ggttttgtcc tcatcgtcac ggaaccgacg ctggcgggag
     3781 aacacgatct ccgacgcttg gcggaactgc tcgccctgtt caagataccc gccgggctct
     3841 gtctcaacaa atgggatctg aatcccgaaa tggcggcccg gatggaggcg gccggccgcg
     3901 acctgggcat gcacgccgtc ggcagaatcc gcttcgatct gacaacggtc gaggcccaac
     3961 ttcagggtcg caccgtaatg gattatcccg ccagtccggc ggccgcggat atcgaggccc
     4021 tgtggaaaaa cgttttaaat ttattgagaa aaaacgattc aggaggctga cgaatagatg
     4081 agcaatacag aagatgccct tcccctgtac gggaaagggc agaaacagca acaggaaaac
     4141 cgcgaccgga cggaaaaaca ccagcaggag gagaaacagc tccgggaaag aatggcgcaa
     4201 attcgccaca agatcctggt cctttccggt aagggaggag tgggtaaaag caccatcgcc
     4261 gtcaatatcg ccgtcggtct gggaatggaa ggaaagaagg taggtctgct ggatgtggac
     4321 tttcacggcc cgagcatccc cacgcttctc cggctcggca atcagaccct gcgcaccgac
     4381 ggcaaaaacg cctttcccct ggattacgta tatgggatca aggtggtttc cctgggcctc
     4441 ctcctgcctg accaagacga ggcggtcatc tggaggggtc cattgaagat gggcgttatc
     4501 cggcagttcc tcgcggacat ccagtggggg gatttggatt atctgatcat cgatttcccg
     4561 ccggggacag gcgatgaacc tctgtccgtg gcccagatgc ttccggagag cgatggggcc
     4621 gtaatcgtca cgacccctca ggaggtgtcc ctggccgacg tccggaagtc catcagtttc
     4681 tgccgccagg taaaggtgcc cgttctcggc gtcgtggaaa acatgagcgg cctcaactgc
     4741 ccccactgcg gaaaactaat tgaacttttc aaaaccggcg gcggtgagtc catggccaag
     4801 aagctgaagg tcccctttct ggggcgcatc cccttggaac cggccgttgt ggaggcctcg
     4861 gacagcggtc ttccgttcat ttaccactac aatcaaaccg gcgcggccca ggcctttcgc
     4921 gaggggatca tcaaaaccat cctggccctt gacgaccgtt aggaaatggc ggtatcagcc
     4981 gtaacagcaa gcggcatttt gcccaacggc tgatattctt gaataatttt tttttaaaga
     5041 aacaaccatg aggtgaaaag agatgcaatt gatgcgaatc gccgtcccca ccgtggacgg
     5101 ttatctgtgc ccccacttcg gccactgcgg cgccttcgtc atcgtcgacg tggacaagga
     5161 cgaacgaaag atcctgaaaa cggaaattca tgaggctccg ccgcacgaac cgggcctgtt
     5221 gccccgctgg ctggcggaaa agggagctga cctgatcatc gccggcggca tgggcgcccg
     5281 tgcccaggat ctcttcaacc agcgggggat cggcgtcgtg gttggcgccc cctccgaaac
     5341 cccggaaaag atcgtcggcg actatctcca gggagcattg ttctgcggcc aaaatctctg
     5401 cgatcactga gccttgcggt gggaatccag acatgccgat atacgaatac gagtgcgcgg
     5461 attgcgggaa gacctccgaa tttcttgaag ggataagcgg ccaagaagaa gcgccccggt
     5521 gccttttctg tggcggcgcg gaattgcgcc gcctcatgtc ccccagcatt cttcctcgga
     5581 gcggcgcctt ttcggcttcc ccggcgggca ggacatgctg cggccgcgaa cagcgctgtg
     5641 acgccccgcc ctgcgcgggc ggcggaggct gccggagatg agcagcggga aatcggctcc
     5701 ggaacccagc gcaaaccggc gggagatcga cggtgaaacc ttccaggcca tgatcatgaa
     5761 gatccgccgc cggccgcggc agcaaatatc ggggcattgg ttcaacatcg tggaggcggc
     5821 gggtcatcgc tttcttcaag ggcccctgaa cacggatgtc cgggggcaaa ggaggaacgg
     5881 ggggctggcc tatatcaccc ggttggtccg caccgaggat atcgccggct tctggccgga
     5941 aacgacgggc cgcctcctcg cccccgagga gtgtcatgcc gccgccgcat tcatatcctc
     6001 cctcgacggc cgggacgtag aattgatcgt tccgctacgc aatcgtgccc tgccggtccg
     6061 ttgggagaga ttcgaagatt cccgggagta tcccctttat gccgcccata tccacccacg
     6121 gatattgtcg gccctgtcgg aaattacgtt tcagcgtctc ctcgacgtcg ggtgcgggtc
     6181 ggggaatctg ctggcggcaa tccgccggga taatccccat gcctattacg ccggtttcga
     6241 catcggcccg gacaatgtgg aagcagcaaa agaaaaggga ttagaaaata tccgcgagga
     6301 aaaaggagaa aatgtcgcca cggtgtatgc catggcgcct cccttcgacg tgatcatctt
     6361 ctgtgggatc ctgaaccggc agatcatgga tggcgacagc gcccggacga ttttgaaaaa
     6421 gagcctgacc cgcttggcga aagggggact cgtgatcatc accggctact cctcctgtca
     6481 cttctgcgcc cgggaactca agaagaaggg attgacggtg ctcaataaga gcatcccgga
     6541 gaacatcttc cgagactacg atgaattcta tctccggcag ttctatgtgg ccagaaagga
     6601 ttgacgcccc cgtataagtc ataatcagac ataatcagga tggaacggat aaaagaaagt
     6661 ttgatattta atttcttatc ggccataatc gcctcaccgt agcgatatgg tcagggttgt
     6721 tcaattagcg gccgctttac ggccaaggag gatgccgatg aaaaaagtcg ccctgttcgc
     6781 cttcaacggc gaagcgatgt gccttgtcca cgttctgctc aacgccctgg agatgaagga
     6841 caaaggttac gaggtcaagg tgatcatcga aggaacggcc accaaactaa tccgagacct
     6901 gacggctgct ccgacaccct ttacccctct gtacgagaag gtgaaggccg ccggcctgat
     6961 tgactgcgtc tgccaggcct gctcgaaaaa gacggggagc tacgacgagg cgacggcgca
     7021 gggattgccc gtgtgtgggg aaatgatggg gcacccgagc atggcccggt accgtgaggc
     7081 cggttatgaa ttgatcgtct tctaataaac acggcggccg tcgtattgga ttatcaccag
     7141 cggggcattg ttcccggaac ttggtaaccg gcggccattg caggcataac gatgagaaaa
     7201 acaaggggaa aaggggtgga agccatgccg gccatgccgg caagcggcac ggattgcccg
     7261 gcgaccggag cggcggtgat ctatcatccc atcggcatta tccattcccc cttcagcgat
     7321 tggcggggta tgcccatcca gccggcgggg gcccggggcg tcaaaggaag ggtcgaaatc
     7381 gccgctccct atcgccaggc tctgaaggat ctggaatctt tttcccatat catccttctg
     7441 tatcacttcc acctctcgaa ggattaccat ctcgaagtta tacctttcct ggacaccgtc
     7501 ccccggggac tatttgccac ccgggccccc cggaggccca atcccctcgg actgtccatc
     7561 gtgaagctca cgggcatcga gggtggtgtc ttgcatatcg aagatgtgga tattgtaaca
     7621 ggaactcccc ttctggacat caagccctat gtgccggcct tcgatatcgt tacggaggcc
     7681 acgggcggct ggactcaggg taaggagaaa gaggtagagg taatgcgctc cgacggccgt
     7741 ttccacgcgg cttccgagcc ccggtgaaac ccggtggatg gggccggaaa agggggataa
     7801 acttaagcaa gctttatcac atcaagcagt taaattcagc gaagctgaat aatattgata
     7861 atatataagg aggcgttagg agatgtttca tgaagagcga tgcgactttt gcggcgactg
     7921 tctggcccgg tgccattacc tagatttcga caacaagacg ggcggtgagg ccttcgaaaa
     7981 actcgtccgg ggagaaaaat cagactggat atacgactgt gtgacctgta ttgcctgcaa
     8041 cgaatactgt cccaaggatg cccggccttt cgatctcatc ctgcggcgga tggaggaggc
     8101 gggtgacttc acggatccga agctgaaggc ccagatggct gaccgcttca aggccgaggg
     8161 cgagccccgg cccatggaac ccaaagaccg ggtaatgtcc atctgcgtca tggacgggag
     8221 catgccttgg gcgatccagg gcagtctctt ccaggagctg cctatcctca aaggccgcca
     8281 ttatttctgc aacgtcctgt tcgcccacat gggggacgaa tccatcatgc gggaccgcct
     8341 ccaggggctg gtgaacaatc tggccaaatc gagggccaag gagatcgtct tcgtccatga
     8401 cgactgctat gccctcctgg ccggcatggc accggaattc gggatcaagc tgcccttccg
     8461 ccccatttcc atcctggaat acctggcgga gtacctgaaa aatcacccgg accgtattac
     8521 cagactgaat atgaaggtcg cctaccagcg tccctgcgcc tcccggtaca ccccggaaaa
     8581 ggatcatttc ctcgctgaga tcttctccct catcggcgta gagcgcgtcg cccggaagta
     8641 tgacgggatc aacgccatct gctgcggtgt agaactggcc ggtccgaacc tgaagctctt
     8701 ccccaggggc aagaacttcg cccccttcca ggaaaagaat atccaggacg ccaaggactc
     8761 cggcgccgaa gcgattgttt acctatgtcc catgtgcttc cggaccttga acaaaaaggc
     8821 gcgggaagcg gggatgaaga actacatgat cagcgacatc tgccggttgg ccctggggga
     8881 gaccctcccg gaggacaaac cggcttagcc ggcaaacatc cggatcccgg ccggctcgca
     8941 tcgggagtga cggggaaacg accccagacc gtccgaagac accggcaacc ccgtcagggg
     9001 ggaggcatcg ttttgcctcc tccccacccc tgaggtctcc gggtaccgac ggccggttgc
     9061 atgacgccgg agcgataaga aaacgtcttt tatcccgact ttccgcctcg acactgggca
     9121 gaggaaatcc ggcggtaata tgaagaagga tacagggagc attaatggaa gacattggca
     9181 atgaaattct atctccacag ttaatcgatc acgcccggca gccccggaac aagggaatcc
     9241 tgccggggta caacgccgaa ggccagataa caggaccctg cggcgatacc atggccttct
     9301 ggctggaggt gaaggacggc acggtgaaaa atgcctcttt ctataccacc ggctgcggca
     9361 cgtctctggc ctgcgggagc atagctacct gtctggtccg gggcatgcgt gtcgcggcgg
     9421 cggccgccct ctcccaggaa gacattctta atgccctggg aggtctgccc ccggaggcgg
     9481 aacactgcgc cctcctggcg gccaatacca tgcgggccgc ctgcgagaat tacctcaaag
     9541 actcaaggcg agcggaaaaa tagcgatcca ctccccctaa ggcgccctgt agtgaagtaa
     9601 cttgagtgca gttagttgta aggtcggacc tcgtcgacaa gatttgggaa gcaggtaatc
     9661 attgaagaca ggaggattga gaaatggctg ctcccagctt gaaaccggtg gataacatag
     9721 agatcctgtc ccttcaggac aactacatcg agatgacagc cacggacaac agcgccgtcg
     9781 tgaccagggc catccccctt aaggacggtt gtttccgcct gtcggtcctt gcggaacacg
     9841 gttttgccgc cgttgtcaag accggtgtca acggcaagac gcataccctg ctgttcgatt
     9901 tcggtttttc cgaaggaggc gccgcctaca acgccaaaat cctgggagtg gatttgggcg
     9961 cggtggaggc cgtcgccctg tcccacggcc acaatgatca caccggtgga ttcgatcaat
    10021 tggcggccct gctcggcaga agagagatcc ccttcttcgt ccatccacag gtttttcgga
    10081 aaccccgcta cctgaagata cccgtgcggg acatgaagat ccacttctcg cccctgactc
    10141 gggaacagat cgccgcaacg ggcttgaagc tcatcgaaac ttcggaaccc gtcaccttcc
    10201 tcgacgatac ggtcctgttt ctcggcgagg tcccccaccg gacggatttt gaaaagggat
    10261 ttcccatcgc tcgctatcag gaagacggac aggaaaaatg ggaccccatc gaagacgaca
    10321 cctccgtggt catgcacttg aaggacaagg ggctggtggt actctccggc tgcgcccatg
    10381 ccggtatcgt gaacacggtg aactacgccc gggagatcac gggcgtggat aaagttcacg
    10441 ccgtcatggg tggatttcac ctctccggca tctacttcga ggggattatc gaccggacgg
    10501 tggaagagat gaaaaagatc gatcccgact atgtcatccc ctgccactgc acggggcgca
    10561 acgccatcat ggcctttgaa agggcgatgc cggaaaagtt catcctcaac atggcgggaa
    10621 cgaagctgac cttcggggca tgaatcggag cttccttatt ccaaaatgat tcctctgagt
    10681 tcgggaaagg agagagacat tcggaaaatc gatcagggac aatgcctgaa actttattcc
    10741 gtcgggatgg aatgcatgat gaaaatggca ctcaaaaaga tccctccgct gctcatcgct
    10801 ccggagccgg gatgacttcc cggagatatg ggtaccctca ccacccatat ctccgggaag
    10861 cgcgagggga ttgggcaagg tcgtctccct caatcaagaa tacaacatag atcggtcttt
    10921 atcattcagg aataatcagc gagttgtcag tccagtgctg tccagatgtc ctgcagggcc
    10981 gccaggaggg cttcgttctc cggccgtttc cgaacggcaa cgcgaaagaa acgccggtcc
    11041 agaccggtga aattgtcgca gcggcggatg gcgatcccct tcttcagcag tttcgccgcc
    11101 agcccgggag cgtccatttt ccggccgtcg atccgcgcca aaaggaagtt ggcctccccg
    11161 ggatagacat ggaaggcgaa acgatcccgc agaccttcgc tcaggaagcg ccgttccttc
    11221 tcgatcaggg ccagtgtccg gcgacggtaa tcgtcatcgt ccaaaaccgt ctctccggct
    11281 gcctgagcca gggcgttcac cgtccagggc agaatgaagc cgccgatctt ttccatcacc
    11341 gccgccgctc ccgccgcgaa ccccagacgg aggccgggga tggcatggaa cttcgtcagg
    11401 gaacacagga cgatgacatt gggagggcgc cggaccaaga ggcgatccat ggcgggtaca
    11461 aaatcggcaa acgcctcgtc aatgacgaag accgtatcgg ggtggcgccg ggcggcggcc
    11521 cggatcgcca cggccgcgca gctcaaaccc gtgggattgt tgggccggcc gatgaaaacg
    11581 atttcatcgc cctgcagatg gtcctccagt tcatccatct ccaggatgaa gccccgatgc
    11641 tcactcaggg cgaccttcac cacctccatg cccgccaatg ccgacgcgac ggcataatcg
    11701 gcgtaagacg gcacggggat aaccgccctg ccccgggaaa gcgcccgggg cagggcatag
    11761 aggatctccg tggaaccgtt gcccacgatg atttcctccg ggggaatccc gtagcgccgg
    11821 gaaacagcag caatcacccc cgccgcccgg ggatcgggat aatgaaggat ttcgttgagc
    11881 cggcggcgga taaggctccg aaagcccacc ggcagcccca ggggattgat gttggcggaa
    11941 aaatccagca gttcatcctg tgaaacaccg gcccgggcgg ccaggcggcg gatatctccc
    12001 ccgtgctgga attgcgatcg cggcatctct aacatcctcg tctctccgtc ccgaccccgc
    12061 cgtccccgaa tcccccccca cgccggcgct atccgtgccg ggaggacgcc ttgcggctca
    12121 cgcgtctgcc tgatcgtgag cgctcgttcg ccggtcagga aacgttttca ggcaaacaga
    12181 ccggacgtag cgtagcggag cgtttccgcc atgtcaacga caatcccgaa tcatgataat
    12241 caggaatccc ggcggcggac cgacttacta tccgccggcc gcggccgcct cttcccgctt
    12301 atcgcgccgt cggcccttca aaggagttga caagccggca gggattcctc taaacagaaa
    12361 aaacctgttg aaacgtcacg aagcacaaac agtagtattg acgatatttt tcgtagtgag
    12421 tcatggttga cgacatcgta aaaggtcaaa aaatgttcca tttgggacgg catcgtaaaa
    12481 agatccgcag gcgaggcgcg cgaatcctga ggaatgaggc gtactgggac gtacgtcgca
    12541 gtgacgaggg atgaagcgca acgaagcatc cggacttttt acgatgccgt catgatttag
    12601 agtttgttct taaataacat tgcgataggg cgcgctcgta tttatgccgg atttggccgc
    12661 gccgattaag caaaaccgca ggcgtagtgg cgctacgttg aggattttgc gaaggttgaa
    12721 ggcccatgcc ggatgcccac cttatcgcca cgtcatcaat catggccggc aatcccaggg
    12781 ggaaatcctc gatgaccgac gtctttttct atgcccttct cgccgccgac ggcctctttc
    12841 tggtcctcct cgtcgcattg ctgttcaaat cgtcgtcccg gccgctggcc gcgatcgacc
    12901 accgcctgga aggcatggaa acgctccagg aaaggctgga gcggaccctc cgggaggatg
    12961 gccccagaga tcgggaagag ttgaaccggt ccctccggga cgtgagcgag tcgctcctgg
    13021 tccggctggc ggaaaacatg tccctgcaga aggaccagtg ggagatattc tcccggcaat
    13081 tgacggacct tgccgtccag agcatccaga agctcgacag gataaacgaa agcgtcgctc
    13141 ttcagttacg caatctccag gaggaccagc gccggcaatt ggaggcgatc cgcgccacgg
    13201 tggacgaaaa acttcaggac accctggaaa agcgcctcgg ggagtccttc cgcctggtga
    13261 gcgaacgcct ggaaacggtc cagaagggtc tgggggagat gcagaccctg gccgccggcg
    13321 tcggggatct gaagaaggtc cttaccaaca tcaagacccg gggaaccttc ggggagatcc
    13381 ggttgggcac cctcctggaa gagatactca gtcctaacca atacgccgtc aatgtggcca
    13441 ccaaaccgga cagcgaccac cgggtggaat tcgccgtgcg gatgccgggg cggcacgatg
    13501 atccccgaca ggtcgtctgg ctccccctgg atgccaagtt cccccaggaa gatcatctgc
    13561 ggctcctgga ggctcaggag cagggaaatc ccgggcaggt ggaggaggcg tcccggcaac
    13621 tggaacggac gctccgggac atggccaaga gcattcggga caaatatctg gcgccgcctc
    13681 atactaccga tttcgccatc atgttcctgc ccacggagag cctctacgcc gaagtcctcc
    13741 gccgggacgg actcttcgcg cagctccagc gggaatttcg ggtcgtgatc accggcccca
    13801 ccactctggc cgccctcctg aacagcctgc agatgggctt ccgcacgctt gccatcgaga
    13861 ggcgctccag tgaagtatgg gaactcctgg gcgcggtgaa gacggaattt gccaatttcg
    13921 gcggcatcct ggaaaagaca cgcaaaaaga tcatggaggc gggcaatacg atcgaaaagg
    13981 ccgctgtccg ctcccgggcc atcgagagaa ggctgcggga cgtccagggt cttccggcgc
    14041 cggaggatgg aggtggggaa tccggagcgg aagacgccgg cgataacggt gctccctata
    14101 accgaagcta atcaaagcat ggcgggaccg atggtcgttc ccgcctttgc ggcggcggcg
    14161 ccaggcgcgg ccgtccccgc aggatcatca aagcgccggg gcgaggaaac cgccttggcg
    14221 ggggaaagga gctttctctt gaagggaacg atcgtcaata ccggagcggt catcgcgggg
    14281 agcctcatcg gtctggcagc gggaaagcgc atgcccccgg gcctcaaaac gatcctcatg
    14341 cagtgtctgg gtctctccac catcctcatc ggcctgcaga tggccctttc ggaacgggac
    14401 gtgatccccg ccatcggctg cctgctgctg ggcgccctaa cgggagagct gctcaaaatc
    14461 gaaagtggga tcgaccggct gggggaatgg ctgaaggcta gggcccgttc cgaatcatcg
    14521 acctttgtgg agggcttcgt aaccgcttca cttctctatg taacgggggc gatggtgatc
    14581 gtaggttcca tccaggacgg cgccaccggg gatgcgacga ccctttatat caagtccctc
    14641 ctcgatggag tcgcatccat agcctttgcc tccaccttgg gcatcggcgt cgccttttcc
    14701 gccttgagcg tcctgtgcgt ccagggcgtc ctgacgctgc tggcggcgca cctgagcttc
    14761 ctgcagcaac ccgcggtact gggcgccatc accgccacag gggggctgct catcgtcgcc
    14821 atcgggctca acctcctcaa cgtggcgaaa atccgcatcg gcaacctgat ccccgccctg
    14881 atctacgccg ccatctgggc cgtgtacggc gggtaatgag gaagggattg tttttaagcg
    14941 cgacggtcga tcgaaacatg gaaaccgaaa atccgcgctc ggtccgctcc tcacgatgag
    15001 gcgcagcttc agcgcatctc ccgaatgacc ttgccccgac tcaaaaagga aatgccccga
    15061 ttgccttcga gattcaacat gatggattcg atcagcggtt cactgaccgg tacatcggac
    15121 agccactcca ccaggaaatt ggcccccgta ccgctggtgt ccgactgggg aataaaataa
    15181 ttcaccgctc ccaggggagg cagggggtgt tccccaacga ggaattcctt gacgagcttg
    15241 ccgtcattgt tgaaatagag gacctttgtg accttgatgg gatttttcag atcggtgttg
    15301 tggatggcaa gaaaggcgct cagatcgaac ttcttgctct cgatataggg gatgttggaa
    15361 taaatgggca cgtagaggac ctgcccccgg agcttattct ccgcggtatc ggccaccttg
    15421 agggtttcgg ggcggaaatc ccctgccgcc tgcccgttca atggcggcgc ttcctttccg
    15481 gaacaggcga ttacggagaa aaacagcgca atgatcgcga tggtcctgat gagccggccc
    15541 atattattca tgattccctc ctcactgcca tatctggacg acatataaaa ggggaatgga
    15601 ggccctgtca atataattat aaacgagaaa gcccccagtc cgaatccgga gggggggctt
    15661 tctcctgaat atgaaggctc gattatttct ttgccgccgc tacagcatcg cgaagctttt
    15721 tccccgcggt aaacttgggg acctttttgg ccttgatctt gatggccttg ccggtctggg
    15781 gatttcttcc ggtgc
//
LOCUS       DAOSMF010000032        15267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_4620_length_15267_cov_18.055088, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000032 DAOSMF010000000
VERSION     DAOSMF010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 15267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15267
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4620_length_15267_cov_18.055088"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2216)
                     /locus_tag="PLT63_05180"
     CDS             complement(<1..2216)
                     /locus_tag="PLT63_05180"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HOD98151.1"
                     /translation="MAEQDERMEIPGGGAVRMEGTAVPRTLQDGRLLHADFGKYKKWL
                     RERFSMAGEVGEDFQKTKISGLYTLAAPLGIKESELARTISEFLNLMHRKTMDYDEYL
                     TDLLPMEFCRWNSVAPILDAESGGAFLISNPFDWELLDVLKKNTPADQPLRLIVADPL
                     YIRSLLDRKNDKARMQAARSVGEVEIAKEDEPDVKMAPVSMPVIEDVGPVSAADVEKR
                     PVVHVSNNILYTAVMERSSDIHIEPKEKETVVRYRIDGDLRDIFRLKRQTGVMVISRL
                     KALAGLDIAERLKPQDGAVEILVGKRTFKLRLATTSTPSGESLIIRILEPSAKPKDLG
                     ELGMTKEQVRTMMDLATRRYGLILVVGPTGAGKTTTIYSFLSHVDTKTRSLISVEDPV
                     EYRIPEANQQQVNEKAGVTFDALLKSSVRQDPDILYLGEIRDNFSARISVDFASTGHM
                     TISTLHTNNATTAIFRLERLGIVREVMAEALLGIIAQRLLKRLCPHCKRVAPITPREA
                     EMLRPFTDELPEYVAHPVGCPKCREGYFGREGVYEIIAFDPDLLERIRNGMAISELRE
                     FIHRRGDYLISHHAVQKVKDLIFPVKDVYDKVLVEEIQMTPKDEEVKVEKKEDPGQKA
                     GAPAILVVEDDEDNRLLISRILSGQGYEVAVAGDGIDALMALGTKEFDLILSDINMPN
                     LDGFKFLEIINQKGVRAPLMFLTARADEEDEVKGLELGALDYLKKPIKKDALLMRV"
     gene            complement(2229..2558)
                     /locus_tag="PLT63_05185"
     CDS             complement(2229..2558)
                     /locus_tag="PLT63_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HOD98152.1"
                     /translation="MTIGEGDLRSDRITVVVEEDLRELIPGYLENRRKDITTIREALA
                     GSDYEAIRSLGHKMKGSGGGYGFDAITELGHDLEAAAKSEHAEEISRLNDALASYLER
                     LEVVYQD"
     gene            complement(2746..4119)
                     /locus_tag="PLT63_05190"
     CDS             complement(2746..4119)
                     /locus_tag="PLT63_05190"
                     /inference="COORDINATES: protein motif:HMM:NF024314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOD98153.1"
                     /translation="MRRQGVPHRINGPTLAFLLALVLVASGCGNFDVIRDIRELPQNH
                     FAYLPWDNDGRLRVDKDWQEEMKERYLENHFLPWRGEAPRVTSDEVKAAWLPFVPDPD
                     DGSRSARRRRSRIRRAAANTGLHNYPNASQPGITTATVHLRHLPIAVPPADAPGRPGR
                     RDPLDKLQVSSVPPGTPVHIYHFSKDGVWALVETGFARGWTKRKGLAAVDPDFMHRWE
                     RGPQAVIVRDKTPLRRRDGRFLYRAPLGALFPMATAPGEKLEVYAPVRGKRGGVRIHT
                     AVLPGEAAVFPLPLTDANLATLGNELIREPYGWGGMNGRRDCSAMIRDLLAPFGIWLP
                     RHSADQAREGGTFIDLTGLATAEKKRLIMDRGIPYLTLLWLKGHIMLYIGSPSGEPLV
                     FHNFWSVRTISAAGRPGRKVVGRAAITTLYPGREFNGAADPEGPYLAYLQGMTLIGPV
                     IVAKLEN"
     gene            4364..4849
                     /locus_tag="PLT63_05195"
     CDS             4364..4849
                     /locus_tag="PLT63_05195"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOD98154.1"
                     /translation="MVTANMLKEINFFKDFSHEQLEKLAGIATEESHAAGSQMYKKGD
                     PARSLFIVVEGKVVMSLESYLGPHRPPMQVTVDTVVKGEVMGWSAVVEPYIYTLGALC
                     IDNTRLIALEAGKIRDLMEEDCDLGFKAMKAIAKIIASRLSHTRIILVGERGLSQLTE
                     Y"
     gene            4871..5077
                     /locus_tag="PLT63_05200"
     CDS             4871..5077
                     /locus_tag="PLT63_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_258239556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:4907..4909,aa:Sec)
                     /transl_table=11
                     /product="UXX-star (seleno)protein family 1"
                     /protein_id="HOD98155.1"
                     /translation="MEEKIVIYGKSGUPYTDQARSVYGSRAVYVDVKKDAERLQEMLK
                     LSGGKRQVPVIVEGGKVTVGYGGA"
     gene            5148..5615
                     /locus_tag="PLT63_05205"
     CDS             5148..5615
                     /locus_tag="PLT63_05205"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOD98156.1"
                     /translation="MKNKTLEAILQKAIGNEEAAFRFYMDLYHAVEDKAAKNTLVFLA
                     DEERKHRDYLIKYRDGVYPENALTIAEEGTAKIREFEEKPAIKKTARDKDIYLVAAER
                     ELKAYNFYTKLAEIHPEGEARDILTRMANEELKHKEKMEYLYDNTVFAQTEGG"
     gene            5704..>6185
                     /locus_tag="PLT63_05210"
     CDS             5704..>6185
                     /locus_tag="PLT63_05210"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF013979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HOD98157.1"
                     /translation="MKSLVPTKVFFTKGVGTHKDELHSFERALRDAGIEKCNLVLVSS
                     IFPPGSKMISRAQGLKAMVPGAITYCVMSRACSNEPKRLLAASIGCAIPADKTSYGYI
                     SEHHAFGQTEKQAGDYAEDLAAAMLASTLGIDFNVDESWDEKKEIFKISGKIVRTRNV
                     T"
     assembly_gap    6186..6285
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6432..7316
                     /locus_tag="PLT63_05215"
     CDS             6432..7316
                     /locus_tag="PLT63_05215"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOD98158.1"
                     /translation="MHRQHRADPVAGPRTAVQAIDGVEIPYLFYEADGPSLLFLHATG
                     FLPWLWHPLARDLAPPYRVIAPYFCDHRETTPEDGGLSWLTLARDLADFYAALHLDRP
                     FLVGHSMGATVLTLANAVFGLAARGMVLVEPIFLPQDFYRLEITVDQHPLAAKSIKRQ
                     NYWPSVAAAGDYVRSRSLFRNWDGEMMDLYIKYGMKSGDGGGVQLTCSPQREAAIFMG
                     GMQYDPWPELVKVTCPVLVLEGAESDNRHFIDLRQATSFFPRGEHRMIAGAGHLIPME
                     KPRELAEIIRGFCDRLRD"
     gene            complement(7350..7778)
                     /locus_tag="PLT63_05220"
     CDS             complement(7350..7778)
                     /locus_tag="PLT63_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98159.1"
                     /translation="MSELLDVSTWSILQWALVVLVAGFIGQFGKSFAKFLMEKLNAAQ
                     KGKTAVKTGEHLPDPAIKKEDEGNDGSADGGREKALPPAVVGEAPAPTAIAVPAVPQV
                     QKTPPSPVEAGSSAKEEKKLLKAQLKAQKKAAKAANKESS"
     gene            complement(7956..8474)
                     /gene="mobB"
                     /locus_tag="PLT63_05225"
     CDS             complement(7956..8474)
                     /gene="mobB"
                     /locus_tag="PLT63_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HOD98160.1"
                     /translation="MNKTIPILSIVGKSNSGKTTLIEGMIPALIRRGWRVATIKHNRH
                     GFAIDHEGKDSWRHKRAGARTTVIASPHQVAVVEDTERDYEIAELRERYIRDVDLIIV
                     EGYKQNPFPKIEVFRPSLKRERLCGPDDNLIAVASDEPLPETETPNLALNDIDGIVAW
                     VEESLGLKAGEQ"
     gene            8786..9580
                     /locus_tag="PLT63_05230"
     CDS             8786..9580
                     /locus_tag="PLT63_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98161.1"
                     /translation="MDLQLMDSVRQLLAEVERLAGEKIAVAKKPELAAPIDFHLGGRG
                     EPRLVFDYRPHPETDVNFLVANQTGHLLRFFSAPAAARFVSVAGRRTMASFIMETESE
                     LERLVAIFGRDKVRRMIARWYQGVVFQLTRMPADIMVNKWLYDHCPELRRLQLHNISV
                     QRRQALQSLSPDVRRATPARIYRAANIMNFVFFKSLEDHFHGDFIGPYHKTIFILDGA
                     PLVRITAARRKDDHAGDRAMIDAWAAALALDHWYEWQPYESFSSPY"
     gene            9793..11625
                     /locus_tag="PLT63_05235"
     CDS             9793..11625
                     /locus_tag="PLT63_05235"
                     /inference="COORDINATES: protein motif:HMM:NF014598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HOD98162.1"
                     /translation="MTTTVVVVIGFLIYMTLYFTYGKKIERDVVKVDDSIETPSKRLY
                     DGVDFVPAHKAVLFGHHFSSIAGAGPLVGPAMAMCWGWLPGLLWVWIGNVLIGSVHDY
                     LALMASVRYDGKSVQFVASDLISKRTGRAFYWIVFFLLILVVAAFANVIGSMFAKTPE
                     ISAASIYMTIAAIILGILMYRVKLNLPIATVIGIVLMAAAIWISTYAPVAASYEVWMV
                     VLFLYIIIAAAIPVNVLLQPRDYLNSFLLYFGIAIGFIAAIFTFRGFEMPMYSTFSPI
                     LLGGKPTPFWPAIPLVIACGSLSGFHSLVGSGTSSKQLAKESDGLFVGYGAMFTEGFL
                     STVIICAIGGFGYTAIQNAHVAAAAAAAAKAAAAGAVAAAGAAGAAAAGAAAGAAPAA
                     PPLALTLDNWGALYTKTTETLKLSPPNMIVQCFADMTAASFLAFIPTKIVKVIAGLWI
                     SAFALTTLDTTNRLARYCLVEMLEPLKKTSAGLYGTLTNRWTASLIPAAIGIYLAWSK
                     QFTILWPSFSAANQLIASIALMTGAAYVAKRMKSQFAIMAVVPAWFLWFTVTCAILWF
                     MAVVMPGAIAKAAGPGWTIQIIMGIMVILNFLFIYDFTKSRKSD"
     gene            11940..12227
                     /locus_tag="PLT63_05240"
     CDS             11940..12227
                     /locus_tag="PLT63_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98163.1"
                     /translation="MKKSSFLNQCREVLAGVGTAQREAAVSLTCLEVQELENIFALLL
                     LGAFVGLPAPPTFLSVELLPFMDREIRILHQRAEDAHDMLAEMMGALGADG"
     gene            12224..13165
                     /locus_tag="PLT63_05245"
     CDS             12224..13165
                     /locus_tag="PLT63_05245"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HOD98164.1"
                     /translation="MKILFFTGKGGVGKSTLAAAAAWQLSQRHRVLIVSLDPAHNLGD
                     IFGVEIRGRRRRFTAGLYLDEVDLKKMARAYLERETQVLSDTYKYLSTLNLDRYFSVL
                     KYSPGIEEYALLTSIEQTIRKEEGFDYLIFDTPPTGLTLRFLALPQVTVTWIDRLAAI
                     RRQILEKRYTIQKIRRPLREGETRQEIRLKFSEDEDEIMMRLKSLHDNYRRLTGILQG
                     GDCGVVLVFNPDLLSRRESERLIEGLDDLRLPLRLLIQNKVTAENEQIAAAVANSLRR
                     TAGNHMPLRYVALNDALWAGAGKLYDIPEDLEADLEF"
     gene            13155..13841
                     /locus_tag="PLT63_05250"
     CDS             13155..13841
                     /locus_tag="PLT63_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98165.1"
                     /translation="MNFSNLFAREDIVTQPLFYIFVAFSALLGFAFFRGKRVNDRMFR
                     GAFQDLIDVFRPVDQTFTNIGGSIGYHAQLSARKADVVEKVEATITFLPRQALLWMPI
                     SLLIRKYDRLFITLYLRHRPLAEGHLIEKGYAGFRGPKITNAARLQQEQVRWGNSDFL
                     LYYANETIRQRLRQFLNRRETPGTIRHIALVPEEKKCFVFMIPTRGQVAAHLSPVYQW
                     LPSVLKMPGK"
     gene            14106..14627
                     /locus_tag="PLT63_05255"
     CDS             14106..14627
                     /locus_tag="PLT63_05255"
                     /inference="COORDINATES: protein motif:HMM:NF025049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOD98166.1"
                     /translation="MAKKQDLGVFDFAVKAEKDGMDFYMKAAKKFAADQDLKNLFVGL
                     AKEEATHMKIFLDFKAKAEGKGVDQCLKSPEIDAYLESIVQEGLFPKGETVNKRLEKV
                     ETVAQAAAVAMAAEKNAILLYTELAKLSRDKDQKKFFEKIAKEEKSHVVMVANLRADH
                     DPAYAALRFGRFF"
     gene            complement(14757..15029)
                     /locus_tag="PLT63_05260"
     CDS             complement(14757..15029)
                     /locus_tag="PLT63_05260"
                     /inference="COORDINATES: protein motif:HMM:NF013999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HOD98167.1"
                     /translation="MPPEMIAKIEAVLERVRDPESDLPVARLGLIKRLRYSEKERKLY
                     VFMNVYRHLPSCVTCAAIARMIVATIVRDLTAELEAEFPGVAVEFV"
BASE COUNT         3092 a         4679 c         4231 g         3165 t
ORIGIN      
        1 ttgacccgca tcagcagggc atcttttttt atcggttttt ttagatagtc cagagcccct
       61 aattccaggc ccttgacctc gtcctcttca tccgcccggg ctgtgagaaa catgagcggc
      121 gcccgaaccc ccttttggtt gatgatttcc aggaacttga atccgtccag gttgggcatg
      181 ttgatgtccg agaggataag gtcgaattcc ttggtcccca acgccatcag ggcgtcgatg
      241 ccgtcgccgg ccacggccac ctcgtaaccc tgtccggaga ggatccggga gatgagcagt
      301 cgattgtcct catcatcctc caccaccagg attgccgggg ccccagcttt ttgccctggg
      361 tcttccttct tctcaacctt gacctcttca tctttagggg tcatctggat ctcctcgacc
      421 aggaccttgt cataaacgtc cttgacggga aagatgagat ccttcacctt ttggacggca
      481 tggtgactga tgagatagtc gccccggcga tgaataaatt cccggagctc cgaaatggcc
      541 attccgttac ggatgcgctc cagcaaatcc ggatcgaagg cgatgatctc atagacccct
      601 tcccggccga aatatccctc ccggcatttc ggacagccga cggggtgggc cacgtattcg
      661 ggaagctcat cggtgaaggg acgcagcatt tccgcctccc gcggcgtaat gggggccacc
      721 cgcttgcagt gggggcagag ccgcttcagg aggcgctggg caattatccc aaggagtgcc
      781 tccgccatga cttcccggac gatgccgagg cgctcgaggc gaaagatggc ggtggtggcg
      841 ttattggtat ggagggtgga gatggtcatg tggccggtgc tggcgaaatc tacggagatt
      901 cgggcggaaa agttgtcgcg gatttccccg aggtagagga tgtccggatc ctggcgcacc
      961 gaagatttca ggagggcgtc gaaggtcacc ccggcctttt cgttaacctg ctgctggttg
     1021 gcctcgggga tccggtattc caccggatcc tcgacggaaa tcaggcttcg ggtcttggtg
     1081 tccacgtggg agagaaagct gtagatggtc gtcgtctttc ccgctcccgt tggtcccacg
     1141 acgaggatga gaccgtaccg ccgcgtggct aagtccatca tcgttcggac ctgctctttc
     1201 gtcatcccca actcgcccag atccttgggt ttggccgaag gttccaggat ccggatgatc
     1261 aggctctccc cgctgggcgt cgacgtcgtg gccaggcgga gcttgaaggt ccgcttgccc
     1321 accaagattt ccacggcacc gtcctggggt ttcagccgct cggcgatatc cagacccgcc
     1381 aaggccttga ggcgcgagat gaccatgacc ccggtctgcc gcttcagacg gaagatatcc
     1441 cggagatcgc cgtcgatgcg ataacggacc acggtctcct tctccttcgg ctcgatgtga
     1501 atgtcgctcg atcgctccat aaccgccgtg tagagaatat tgttggagac atggaccacc
     1561 gggcgttttt ccacatccgc cgccgagacg ggcccgacgt cttcgatcac cggcatcgat
     1621 accggcgcca ttttcacatc gggttcatct tcctttgcta tctccacctc tcccacggat
     1681 cgggcggcct gcatcctcgc cttgtcattc ttgcgatcca aaagagagcg aatgtagagg
     1741 ggatcggcaa caatgaggcg caggggctga tcggcggggg tgtttttctt gaggacgtcg
     1801 aggagttccc agtcaaaggg attgctgatc aggaaggcgc cgccgctctc ggcgtcgagg
     1861 ataggggcca cggaattcca acggcagaat tccattggca aaagatcggt gaggtactca
     1921 tcatagtcca tcgtcttccg gtgcatcagg ttcaggaatt cggagatcgt ccgggccagt
     1981 tcgctttcct tgatcccaag gggcgccgcc agggtgtaaa gaccggatat tttcgtcttc
     2041 tggaagtctt ctccaacttc cccggccata gaaaaacgct cccgcagcca tttcttgtac
     2101 tttccgaaat cagcatgcaa caggcgccca tcctggagcg tccggggtac ggcagtccct
     2161 tccattcgca ccgctccgcc tcccgggatt tccattcttt cgtcttgctc agccattctc
     2221 gtcccgcctc aatcctgata aaccacttcc agccgttcca gataggacgc caaggcgtcg
     2281 ttcaacctgg aaatctcttc ggcatgttcg cttttcgccg ccgcttcgag gtcatgaccc
     2341 aactcggtga tggcatcgaa accatagccg ccgccggagc ccttcatctt gtgccccagg
     2401 gagcggatcg cctcgtaatc gcttccggcc agggcctccc ggattgtcgt gatgtccttg
     2461 cggcgatttt ccaggtatcc cggaatcagt tcccgcaaat cttcttctac tacgacggtt
     2521 atcctgtcgc tcctcaggtc cccttctcct atcgtcatcg acatctcctc ctggttgtct
     2581 ggtaatacgg cattgcgtct tcagctcgtt ttttatcgtt taaccgaccc gggaattgct
     2641 ttttggactc cagcacccct ggccgccgcc atacccccga gtccgcggta aaactttccc
     2701 gccgctaatt tagaaaatat tgtaaaatga ctaacatatt caatctcaat tttcaagctt
     2761 ggccacaatt accggaccga tcagggtcat cccctggaga taggccaggt atggcccctc
     2821 cggatcggcg gcgccgttga attcccgccc cggataaagg gtggtgatcg ccgcccgccc
     2881 cacaaccttc cggcccggac ggccggcggc acttatggtc cggacactcc agaaattgtg
     2941 gaataccagc ggctcaccgg acggggagcc gatatagagc atgatgtgtc ccttgagcca
     3001 cagcaaggtg agatagggaa tcccccgatc catgatgagc cgtttcttct ccgcggtggc
     3061 aaggccagtc agatcgataa aggttcctcc ctcccgggcc tgatcggcgg agtgccgggg
     3121 aagccagatg ccgaaggggg caaggagatc ccgaatcatg gcggagcaat cacgccggcc
     3181 attcatcccc ccccatccgt atggctcccg gataagctcg ttgcccaggg tggcaagatt
     3241 ggcgtcagtg agcgggaggg ggaataccgc cgcttccccg ggcaaaacag cagtgtgaat
     3301 ccggaccccc ccccttttcc cccgtaccgg cgcatagacc tccagcttct cccccggcgc
     3361 cgtggccatg gggaaaaggg cacccagggg cgcccggtag aggaaacggc cgtcgcgccg
     3421 gcgcagcggc gttttatccc ggacgatgac ggcttggggt ccccgctccc aacggtgcat
     3481 gaaatcggga tcgactgccg ccagtccttt tcttttcgtc cagccccggg caaaaccggt
     3541 ttccaccagc gcccagacgc catctttaga gaaatggtaa atatgtaccg gggtgccggg
     3601 aggaacggaa gaaacctgta gtttatcgag aggatctcgc ctgccgggcc gtccaggggc
     3661 atccgccggc ggcaccgcta taggcagatg ccgcagatgc acagtggcgg tggttattcc
     3721 cggctgggaa gcgtttggat aattgtggag accagtattg gccgctgccc tgcgtatccg
     3781 gctccggcgc cgacgggcgc tgcggctccc gtcgtctgga tcgggaacga agggcagcca
     3841 cgccgcctta acctcgtcgg aggtgacccg cggggcttcc ccgcgccacg gcaggaaatg
     3901 attttccaga taccgttctt tcatttcctc ctgccagtct ttatccaccc ggaggcggcc
     3961 gtcattatcc caggggagat aggcgaaatg gttctgggga agttcccgaa tatcgcggat
     4021 gacatcgaag ttgccgcaac cggaggcaac caggaccagc gccaggagaa acgccagtgt
     4081 cggtccgttg attcgatggg gaaccccttg tcggcgcaaa aactctcctc cgtcttggta
     4141 ccgttaagga ccggcgacaa ttcaggcggc atggccgggg atgtgatccc tcccgccaat
     4201 cgtcccagtc gcatatttgt tattcaccgt gcggttaatt taggcttttt ccctcactcc
     4261 ggcaatctat tttttccttt agacacaaat attttttatg gaattccgga ttaaatcttg
     4321 ctatcatctc tataaaaaag agcaaataat aaggaggtgt attatggtga cggcaaacat
     4381 gctgaaagaa atcaattttt ttaaagattt ttcccatgaa caactggaaa aactcgccgg
     4441 aatcgccacg gaggagtctc atgcggccgg aagccagatg tacaaaaagg gcgacccggc
     4501 gcggagcctc ttcatcgtcg tggagggcaa agtggttatg tccctggaat cctatctggg
     4561 accgcaccgc ccgccaatgc aggtgacggt ggataccgtg gtcaaggggg aggttatggg
     4621 gtggtccgcc gtggtcgagc cttacatcta taccctgggc gccctgtgca tcgacaacac
     4681 cagactcatt gcccttgaag ccgggaaaat ccgcgacctc atggaggaag attgtgatct
     4741 gggtttcaag gccatgaagg ccatcgccaa gatcatcgcc tcccgtctga gccacacgcg
     4801 gatcatcctg gtaggcgagc ggggcctctc ccaactcact gagtactgat gccgcgggag
     4861 gggaaaagat atggaagaaa agatcgtcat ctacgggaaa agcggttgac cctacactga
     4921 ccaggccagg tcggtctatg gaagccgggc ggtttacgtg gatgtaaaaa aggatgccga
     4981 aaggcttcag gagatgctta agctttccgg cgggaaacgg caggtccccg tcatcgtgga
     5041 ggggggaaag gtgacggtcg gttacggcgg cgcctgaggc gtctgaagac ccatcggcga
     5101 tgggtcgccg caccgaagaa aacacaacaa ttcaggaggc tgtgaggatg aagaacaaga
     5161 ctctggaggc gattctccaa aaggctatcg gcaacgaaga ggcggccttc cgattttaca
     5221 tggaccttta ccacgccgtg gaggataaag cagctaaaaa caccctggtc tttttggccg
     5281 atgaggagcg taaacaccgg gattatctga taaaataccg ggacggcgtt tacccggaaa
     5341 acgccctgac tattgccgag gaagggacgg caaaaatcag ggaattcgag gagaaaccgg
     5401 caatcaaaaa aaccgcccgg gacaaggata tctatctggt ggcggcggag cgggaattga
     5461 aggcctataa cttctataca aaacttgccg aaatccatcc cgagggagag gctagggaca
     5521 tcctgacgcg catggccaac gaagagctca aacacaagga gaagatggag tatctatacg
     5581 ataatacagt gtttgcgcaa acggaagggg gttgaaataa attccaaatc atcattgaca
     5641 ccacccacgc aaactgataa gcacggcgtc aatatcaatc cgtgataaag gagggggttg
     5701 gcaatgaaat cccttgtccc taccaaggtc ttttttacca aaggggtcgg cacccataaa
     5761 gacgaacttc attccttcga acgggccctc cgtgatgccg gcattgaaaa atgtaacctc
     5821 gtcctggtct ccagcatctt ccccccgggg tcgaaaatga tctcccgggc ccaggggctc
     5881 aaggccatgg tcccgggcgc cattacctac tgcgtcatga gccgggcgtg cagcaacgaa
     5941 cccaagcggc tcttggccgc ctccatcggt tgcgccatcc cggcggacaa gacctcctac
     6001 ggttatataa gcgagcacca cgcctttgga caaacggaaa aacaggccgg cgattatgcg
     6061 gaagatctcg cggccgccat gctggcttcc acgctcggca tcgatttcaa cgtggacgaa
     6121 agctgggatg aaaagaagga gatcttcaag atcagcggca agatcgtgcg gacccgcaat
     6181 gtcacnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaaaag aaggagatct
     6301 tcaagatcag cggcaagatc gtgcggaccc gcaatgtcac acagtcttcc atcgtcaaga
     6361 acgacggatt cacaaccgtt atcgccgccg ccgtattcgt tttttgaccc tcaagcttca
     6421 tgagcactgc catgcataga cagcaccgcg ccgatccggt ggcgggacca cggaccgccg
     6481 tccaggccat cgacggcgtc gagatcccgt atctgttcta cgaggcggac ggcccgtccc
     6541 tcttgttcct ccatgccacg ggttttctcc cctggctctg gcatcccctg gcccgggacc
     6601 tcgccccgcc gtatcgagtg atcgcccctt atttctgcga ccatcgtgaa accacaccgg
     6661 aggatggcgg tttgagctgg ttgaccctcg cccgggacct ggcggacttc tatgccgccc
     6721 tgcatctgga ccgccctttt ctcgtgggac attccatggg tgcgacggta ctgaccttgg
     6781 ccaatgccgt tttcggtctt gcggcccggg ggatggttct cgttgaaccg atcttcttgc
     6841 ctcaggattt ctatcgtctc gagattaccg tggaccagca tcccctggcc gccaaatcca
     6901 tcaaacggca gaactattgg ccctccgtcg ccgccgccgg cgattatgta cgttcccggt
     6961 cgcttttccg gaactgggat ggcgaaatga tggatctcta catcaaatac ggcatgaaat
     7021 caggggatgg cgggggagtt caactgacct gttcccccca gcgggaggca gccatcttca
     7081 tggggggcat gcagtacgat ccctggcccg aactggtcaa agtcacctgc cccgttctcg
     7141 ttctggaagg agcggaaagc gacaaccgcc atttcatcga cctgcgccag gcgacgtcgt
     7201 tttttccccg gggcgaacac cggatgattg ccggcgcggg gcatctgatc cccatggaaa
     7261 agccccggga gttggcggag attatccgcg gcttctgcga ccggttgcgg gactgactcc
     7321 ttcagacata caggcagggg agcgaacaat catgaggact ccttattcgc cgcctttgcc
     7381 gccttttttt gtgccttcag ttgggccttg aggagctttt tctcctcttt ggcggacgaa
     7441 ccggcctcga ccggcgacgg tggcgtcttc tgtacctgag gaacggccgg gacggcgatg
     7501 gcggtgggcg ccggcgcctc acccacgacg gccggtggta atgccttttc tctcccgccg
     7561 tcggcggacc cgtcgtttcc ttcgtcttct ttttttatgg ccgggtccgg cagatgctcc
     7621 cctgttttca ccgctgtctt gcccttctgg gccgcgttga gtttttccat gaggaatttg
     7681 gcgaaactct tcccgaactg gccgataaac cccgccacca ggaccaccag ggcccactgc
     7741 aatatcgacc aggtgctaac gtccaacaat tccgacattt tacctatcca tcgatccggc
     7801 cgccccaggt gaaatcaccg aaggtgattt atgttaatgt ccccgtaaga agtcatttaa
     7861 ttcctccccc ttcaaggggg agggtgattt gtgcttttta cggactcgtc catgttcata
     7921 aatcccccca acggtcggac cccgggaacc agcgattatt gttctcccgc cttgagcccc
     7981 agggactcct ccacccaggc gacgatccca tcgatgtcgt tcagggcgag attgggagtc
     8041 tccgtttccg gcagcggctc atcgctggcc acggcaataa ggttgtcgtc gggaccgcat
     8101 agacgttccc gcttcaggga ggggcgaaag acctcgatct tggggaaggg attctgcttg
     8161 taaccctcca cgatgatcag gtccacatcc cggatgtagc gctcccgcag ctcggcgatt
     8221 tcataatccc gctccgtatc ctccaccact gccacctgat gaggagaggc gatgacggtg
     8281 gtccgggcgc cggcccgctt gtggcgccaa ctgtctttgc cctcgtggtc gatggcaaaa
     8341 ccgtggcggt tgtgcttgat cgtggccacc cgccaccccc gccggatcaa cgcggggatc
     8401 atcccttcta taagcgtagt cttgcccgaa ttggacttgc cgacaataga aagaatgggg
     8461 atcgtcttat tcatgtccta ctcatataca atatcgtgat cttattcaag gggattcgcc
     8521 ctctgctcca cccgggaacg ctgtcttgcc ggataaattt gttcttcgca caagtagttg
     8581 atattgctcg ggcaacaaaa acaataaagc cgctgtttcc agctggtttc ggccaaaaca
     8641 aattcaccgt gcgcttcccc atccggagct tcccaagacg ggacattaat gctatggaat
     8701 agcggcaacg gcccggccat cgacgggaaa atgtgacggg acatcgtcat gggtgtttca
     8761 tggtcggcaa tgagggttga ttcccatgga cctgcaactc atggacagcg tgcggcagct
     8821 tctggccgag gtggaaaggc tggcgggaga gaagatcgcc gtcgcaaaaa aaccggagct
     8881 ggcggcgccg atcgactttc atctgggcgg cagaggggaa ccgcgccttg ttttcgatta
     8941 tcgcccccac cccgaaacgg atgtcaactt cctcgtcgcc aatcagaccg gacacctgct
     9001 gcgtttcttc agcgccccgg cggcggcgcg cttcgtctcc gtggccggcc gccggaccat
     9061 ggcctcgttc atcatggaaa cggaaagcga actggagcgc ctggtcgcca tcttcggccg
     9121 ggacaaggtg cggcggatga tcgcccggtg gtatcaggga gtggtctttc aactcacccg
     9181 gatgcccgcg gacatcatgg taaacaaatg gctgtatgac cactgcccgg agctgcggcg
     9241 cctgcagctc cacaacatct ccgtccagcg gcgtcaagcc ctccagtccc tgtcgccgga
     9301 cgtccgccgg gctaccccgg cccggatcta ccgcgccgcc aacatcatga acttcgtttt
     9361 cttcaagagc ctggaagatc attttcacgg cgatttcatc ggcccctacc acaaaacgat
     9421 cttcatcctc gacggcgccc cgctggtccg gataacggcg gcccggcgga aagacgatca
     9481 tgccggcgat cgggcgatga tcgacgcctg ggccgccgcg ctggccctcg accactggta
     9541 cgagtggcag ccctatgaaa gtttttcctc accttattga atttatagac aataaaccgt
     9601 tgactttcgc cgcttccggg agtattttcc tcatggctca agggacttct tgctccccca
     9661 tcgccgggct ctttcaaagg gatatcattg gaaaatcatc atctccagtc tgaaacggcg
     9721 caacgggcgc cggcaggttt tatatttttt ataaggttgt tccatcctta ttttgcggaa
     9781 aggaggtagt ttatgaccac aacagttgta gtagtcatcg gttttctaat ctacatgacc
     9841 ctttatttca cctacggcaa aaagatcgag agggatgtcg tcaaggtgga cgattccatc
     9901 gagacgccgt caaagaggct ctatgacggc gtcgatttcg taccggccca caaggcggtc
     9961 ctcttcggcc accacttctc ctccatcgcc ggcgccggcc ccctcgtcgg tcccgccatg
    10021 gccatgtgct ggggatggct gccgggactc ctctgggtct ggatcggcaa tgtcctcatc
    10081 ggttccgtcc acgactacct ggcccttatg gcctcggtgc gctatgacgg caaatcggtc
    10141 cagttcgtgg cctcggacct catctccaag cggaccggcc gggccttcta ctggatcgtc
    10201 tttttcctcc tcatcctcgt cgtcgccgcc ttcgccaacg tcatcggcag catgttcgcc
    10261 aagacgccgg agatctcggc ggcctcgatc tacatgacca tcgccgccat catcctgggc
    10321 atcctcatgt accgggtaaa gctgaacctc cccatcgcca cggtcatcgg gatcgtcctc
    10381 atggcagcgg ccatctggat ctccacctat gcgcccgtgg cggcaagcta cgaagtatgg
    10441 atggtggtcc tcttcctcta catcatcatc gccgccgcca tccccgtcaa cgtcctcctc
    10501 cagccccggg actacctgaa ctccttcctc ctctacttcg gcatcgccat cgggttcatt
    10561 gccgccatct tcaccttccg gggcttcgag atgcccatgt actccacctt ctctcccatc
    10621 ctgctggggg gcaagcccac gcccttctgg cccgccatcc ccctggtcat cgcctgcggg
    10681 tccctgtcgg ggttccactc cctcgtcggc tcgggaacgt cgtccaaaca gcttgctaag
    10741 gagtccgacg gcctcttcgt cggctacggg gccatgttca ccgagggctt cctctccacg
    10801 gtgatcatct gcgccatcgg cggcttcgga tatacggcca tccagaacgc tcacgtcgcc
    10861 gccgccgccg ctgccgccgc caaggccgcc gccgcgggcg ctgttgccgc cgcgggagcc
    10921 gcgggggccg ccgctgctgg agctgccgcc ggggccgcac ccgcggcgcc gccgctggca
    10981 ctgaccctgg ataactgggg ggccctctac acaaagacca cggagaccct gaagctctcg
    11041 ccgcccaaca tgatcgtcca gtgtttcgcc gacatgacgg cagccagttt cctggccttc
    11101 atccccacga agatcgtcaa ggtcatcgcc gggctgtgga tctccgcctt cgccctgacg
    11161 accctcgata caacgaaccg tctggcccgt tactgcctcg tggagatgct ggaacccctg
    11221 aagaaaacct ccgcgggtct ctacggcacc ctgacgaacc gctggaccgc ctccctcatc
    11281 cccgccgcca tcggcatcta cctggcgtgg agcaagcagt tcaccatcct ctggccctcc
    11341 ttcagcgccg cgaaccagct catcgcctcc atcgccctga tgacgggagc ggcctacgtg
    11401 gccaagcgga tgaaatccca gttcgccatc atggccgtgg tccccgcctg gttcctctgg
    11461 ttcaccgtga cctgcgccat cctctggttc atggccgtgg tcatgccggg ggctatcgcc
    11521 aaggccgcgg gccccggctg gaccattcag atcatcatgg gcatcatggt catcctgaat
    11581 ttcctgttca tctatgactt cacgaagtcc aggaagtcgg attgatttta accgattgaa
    11641 aaaccgccgc cgcggggaag ggagaaacat ccttgcccgg ggcggcggcc cgcctttcct
    11701 cccgaccgcc gccggcgaga ggggacggaa agtcgggggc cgaaaacaac ggcgcgggaa
    11761 attcacttaa acgggaaaac gaaagactgt ccccaagatc tcgccgggat ccggcggatc
    11821 atgccggtcg gaagtcatgc cgaccgccgg cgggcattac gggtttcgaa taacgttttg
    11881 aatcgaaacg tccgggctga acacggcaat gacaggagaa cctgagaagc ggaccagtcg
    11941 tgaaaaaatc ttcctttctc aaccagtgcc gggaagtcct cgccggcgtt ggaaccgccc
    12001 agcgggaagc ggcggtatcc ctcacctgcc tggaggttca ggagttggaa aacatctttg
    12061 ccctcctgct cctcggggcc tttgtcggcc tccccgcccc gccgaccttc ctatccgtgg
    12121 agctcctgcc cttcatggac cgggaaatcc ggatcctcca ccagcgtgcc gaagacgccc
    12181 atgacatgct ggcggagatg atgggggcac tgggggccga cggatgaaaa tcctcttttt
    12241 cactggcaag ggcggggtgg gaaaatccac cctggcggcg gcggcggcct ggcagctctc
    12301 ccagcggcac cgggtcctca tcgtctccct cgatccggcc cacaatctgg gcgatatctt
    12361 cggggtcgag atcaggggtc ggcggcggcg cttcacggcc ggtctctacc tggacgaggt
    12421 cgatctcaag aagatggccc gggcctatct cgaaagggaa acgcaagttc tttcagatac
    12481 ttacaaatac ctgtccaccc tcaatctcga ccgctacttc agcgtgctga aatactcccc
    12541 ggggatcgag gaatacgccc tgctgaccag catcgaacag acaatccgga aggaggaagg
    12601 cttcgactac ctcatcttcg ataccccccc tacggggctg accctgcgtt ttctcgccct
    12661 tccgcaggta acggtgacct ggatcgaccg cctggccgcc atccgccgtc agatcctgga
    12721 gaagcgctac accatccaga agatccgccg ccccctccgg gaaggggaaa cgcggcagga
    12781 aattcgattg aagttcagcg aggacgaaga cgagatcatg atgcgtctca aatccctcca
    12841 tgacaattac cgccgactta cgggcatcct tcagggcggg gattgcggtg tcgtcctggt
    12901 cttcaacccg gacctcctct cgcggcggga atcggaacgt ctaatcgagg ggctggacga
    12961 tctcaggctt cccctgcgcc tcctgatcca gaacaaggta acggcggaaa acgagcagat
    13021 tgccgccgcc gtggccaatt ccctccggcg gaccgccgga aaccatatgc cgttacgcta
    13081 tgtggccctg aacgacgccc tgtgggcggg cgcgggcaag ctctacgaca tccccgaaga
    13141 cctggaggcg gatcttgaat tttagcaatc ttttcgcccg ggaagacatc gtcacccagc
    13201 ccctcttcta tatcttcgtc gccttttccg ccctgttggg cttcgccttt tttcggggaa
    13261 agcgggtcaa cgaccggatg ttccgtggcg cctttcagga tctcatcgac gtcttccgac
    13321 ccgtggacca gacgtttacc aacatcggcg gctcgatcgg ctaccacgcc caactgtccg
    13381 ccagaaaagc tgatgtcgtg gaaaaggtgg aggcgaccat caccttcctc ccccgccagg
    13441 ccctcctctg gatgcccatc tcgctcctca tccgcaaata cgaccgcctc tttatcaccc
    13501 tctatctacg ccacagaccc ctggccgaag ggcacctaat cgaaaagggc tatgccggtt
    13561 tccgggggcc gaaaataacc aatgccgccc gcctccagca ggagcaggtc cgctggggaa
    13621 acagcgattt cctcctctat tacgccaacg aaacgatccg ccagcggctc cgccagttcc
    13681 tcaaccgccg ggagacgccg gggacgatcc gccacatcgc ccttgtcccg gaggaaaaga
    13741 agtgctttgt attcatgatc cctacccggg ggcaggtggc cgcccatcta tccccggtct
    13801 atcagtggct cccctcggtt ctcaaaatgc cgggaaaata acggcgctct ccggggcacg
    13861 gggggcgagg gagtttaaca gcggttctca aagggccgga aaggataggg tgaaaacaga
    13921 ttaacgaaac aaaaaatgaa aaccggcagt aaccgcagcg gctgattctt tccggtccgc
    13981 ctccggatca ccgggaagcc tcaccgcccg cggcatggaa aaaatgatgg ggtggcgacg
    14041 gataacgaca agcctcacgg ccgggtccaa cccgcaatcg caaaacatgc aaaggaggtc
    14101 aaaagatggc aaaaaaacag gatttgggtg tttttgattt cgctgtcaag gcggaaaagg
    14161 acggcatgga cttctacatg aaagcggcca agaaattcgc cgccgaccag gacctcaaga
    14221 atctcttcgt cggcctggcc aaagaggaag ccacccacat gaagatcttc ctcgatttca
    14281 aggcgaaggc cgaaggaaaa ggcgtggatc agtgtttgaa aagcccggag attgacgcct
    14341 acctcgaatc catcgtccag gaagggctct tccccaaggg ggaaacggtc aacaaacgcc
    14401 tggaaaaggt ggaaacagtg gcccaggccg ccgccgtggc catggcagcg gaaaagaacg
    14461 ccatccttct ctatacggag ttggccaagc tgtcccggga caaggatcag aagaaatttt
    14521 tcgaaaagat cgccaaggag gagaaatccc acgtggtcat ggtggccaat ctccgggccg
    14581 atcacgatcc ggcttacgcc gccctgcgct tcggccggtt cttctgaaaa attccggcgc
    14641 cttggccgtc ggcaaaaaac aataaaaggg accgcgccgc tcggcggccg tccctttttg
    14701 ccttttccgg aggcccattc ccatcgcttg acctgaggaa attgagcttc gggatctcaa
    14761 acgaattcca cggcgacgcc gggaaattcc gcctccaact ccgccgtcag gtcccggacg
    14821 atcgtggcaa cgatcatccg ggcaatggca gcacaggtga cgcaactggg aaggtggcgg
    14881 taaacgttca tgaatacgta gagcttccgt tccttctcac tgtaacgcaa acgctttatc
    14941 agccccaacc gcgccacggg caggtcgctc tcgggatccc tgacccgttc caggacggcc
    15001 tcgatcttgg cgatcatttc cggaggcatc gagtctccct cccctctttc ccgccttaca
    15061 ttcctaaatc atctcgtgtg acaagccgtc gtgggcgttg ctttcccggg cttcacttag
    15121 gcgttcagcc ctcgtggacc atccgcggtg ccggcggtgt gcgctccatc cactgtttga
    15181 tgcgattggc gtccccatag cgggtcatcg taccctgggc atcgaggagg atgatcagga
    15241 ccgggcgctg ggccacctcg gcctgca
//
LOCUS       DAOSMF010000033        14906 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_4741_length_14906_cov_23.197226, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000033 DAOSMF010000000
VERSION     DAOSMF010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 14906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14906
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4741_length_14906_cov_23.197226"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="PLT63_05265"
     CDS             complement(<1..195)
                     /locus_tag="PLT63_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly PilX N-terminal domain-containing
                     protein"
                     /protein_id="HOD98168.1"
                     /translation="MNPLSHQRTREENRRQGIWRGKNGIALPVALAIITVLFLLGTTA
                     LMMTSTDLKIAGNYREYAKAA"
     gene            complement(282..995)
                     /locus_tag="PLT63_05270"
     CDS             complement(282..995)
                     /locus_tag="PLT63_05270"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOD98169.1"
                     /translation="MLKRNDGFSVPELLIAMLIGLVVVGAVYSLFIMQNRQYRNQGQI
                     TEMQQNARMAMAMMIREISMAGFNQTSAACPNPVTAVRRCTGTTTAANTPCTGITYAG
                     GNEIRFVADLNANCDTTAGTANKDENIAFNVDSSSGVPALNRTSNGVKLPVVEYVDAL
                     SFAYYDGEGNITTDLTAIRKIRISITTRTAQPDPYFTHPTAGDHYRRYQLESFVVPRN
                     LGAYNTTAANTGATGVVST"
     gene            complement(999..1376)
                     /locus_tag="PLT63_05275"
     CDS             complement(999..1376)
                     /locus_tag="PLT63_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOD98170.1"
                     /translation="MKGLPTQAQKRQRGFSIIEIIIAILLVAVAIIGLATMTSTNTQT
                     SDFSKTMTSATTLARDKIEELKGKSYAGLTGGADTSGIYARSWSLEYAPSPHDYKIIT
                     VTVNWTWQGKPSTVELKTIRTRD"
     gene            complement(1466..1951)
                     /locus_tag="PLT63_05280"
     CDS             complement(1466..1951)
                     /locus_tag="PLT63_05280"
                     /inference="COORDINATES: protein motif:HMM:NF023444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family protein"
                     /protein_id="HOD98171.1"
                     /translation="MIIERSNSRGFSLVELLIAIAILAILGAISIPTFRHYLAQRRLN
                     GAVRELQNSLMAMRMQAVTENRWIAMNIGSNHQFTIFRDVNKNGVLDSVEAVSVKDIH
                     PTYYDVTFTSTTGAVLTFYPDGTASTGKICIHGAPGLKPNRLSLNSSGRVRTEETASC
                     P"
     gene            2196..2678
                     /gene="rimP"
                     /locus_tag="PLT63_05285"
     CDS             2196..2678
                     /gene="rimP"
                     /locus_tag="PLT63_05285"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HOD98172.1"
                     /translation="MIRKEMEERIWTITEPAIVGEEMELVCVECLRMKSRWLVRLYID
                     KEGGVTIDDCALISEEVGDLLAVHDVPHEAYTLEISSPGLDRPLVKDRDFLRFQGSKV
                     AVQTEHKIDGARNFKGILQEYRIGEDGGKTLVIEREGKLIAIPREAVARARLESVWND
                     "
     assembly_gap    2790..2889
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2890..3765
                     /gene="nusA"
                     /locus_tag="PLT63_05290"
     CDS             <2890..3765
                     /gene="nusA"
                     /locus_tag="PLT63_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOD98173.1"
                     /translation="EFKDRKLQLINGFVQRFEGGSIIVNLGRAEGVIPVSEQIYREVY
                     KRGERIRSFICDVKRITKGPQIILSRTHPGFLKALFEVEVPEISEGLIEIVSVAREPG
                     KRAKIAVRSKDKDIDPVGACVGMRGSRVQSVVQELRGEKIDIVPYSEDPAKYVYAALA
                     PAKVDRVLIDGENRSMEVIVPDDQLSLAIGKNGQNVRLAVKLTGWKIDVKSESSSAQQ
                     EEDSRQELKALPDITEQQIDGLIKEGIKSLALVAAVDADAIMVVPGVIREQAEGWIKA
                     ATEMTEEAAAAEVRK"
     gene            3837..6476
                     /gene="infB"
                     /locus_tag="PLT63_05295"
     CDS             3837..6476
                     /gene="infB"
                     /locus_tag="PLT63_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041585107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOD98174.1"
                     /translation="MSKKRVYELAKELGVENKELIARLEKMGISVKTHSSTLEDSEVE
                     RVKKEYLAAETHEMVEKRVKATVIRRRAVRPVVEEEASEAEPSAGEAAPPAEPPVAAG
                     EEKPGESEALPAAESPAAPVAPPPAPPVAAEIPPVKAEPPETAPVAPPISEAPAVAAP
                     VSPMAPPPSAPRPPKTEKKDGKLLIMPRKPQNTAEIIRKPLVKKIEAEPGKAPAAPGA
                     LKKPVKWEPEKDKKKGKKPVEVVIGDQFPARKKVLIKKVVDKKVRIEDLERDDRPVRW
                     REEKKAGAAKLKKTEITTPKAIKRRIKIAETITVGDLAKKMGVKAGEVINKLMGLGLM
                     VTINQSLDFDTATLIAGEFGYQVESAGMDFEETFGTTDTGERNLKPRAPVVTIMGHVD
                     HGKTSLLDAIRETNVIAGEAGGITQAIGAYHVQLKDRDIVFLDTPGHEAFTAMRARGA
                     QVTDVVVLVVAADDGVMGQTIEAINHSRVAGVPIIVAVNKMDKPGADPQRIKQALTEY
                     GLVSEEWGGETIFCEVSAKQRVGIEALLEMILLQADILELQADPDRPARGIIIESKLD
                     KGRGPVATVLIQEGTLREGDTFASKTEYGRVRAMIDDQGRRVKEAGPSMPVEVIGFSS
                     VPQVSAEFLCVEDEKKARTIADYWTRKEREKELSATSKITLEQLYQKIKEGVKDFNVI
                     VKADVQGSMEAITEALNKLSTTDVKLRIIHSSTGAITETDVMLASSANAIIIGFNVRP
                     DSRVVEVAEQEGVEIKLYDIIYNLLEDVRAAMEGLLEPVYKEVVQGRAEVRQLFKIPK
                     VGTIAGCYVTDGKISRAANLKLVREGVVVYDGRIASLKRQKDDAREVATGYECGIGIE
                     NFNDLHEGDVIEAYINEQVDRKL"
     gene            6501..6830
                     /locus_tag="PLT63_05300"
     CDS             6501..6830
                     /locus_tag="PLT63_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HOD98175.1"
                     /translation="MVELWLPECRSLKEKRSVLTKLIKRTQNAFNVSIAEVGENDSWK
                     KARVGFCVVGNDRRYINAKIDHIFNYMEGMEIAQVIGTNLEITSYSEWMAGTSGPGET
                     AEKYDDF"
     gene            6817..7191
                     /gene="rbfA"
                     /locus_tag="PLT63_05305"
     CDS             6817..7191
                     /gene="rbfA"
                     /locus_tag="PLT63_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOD98176.1"
                     /translation="MTTFKRAQRVAERIMAELSEILVREVSDPRMKSLTITGVKVTDD
                     LRLARIYFVEMGQDVCRPETRQALQKANGFLRRELGKRLELRYVPEVVFSFDPSFAYG
                     SRIEKILAGIRAGREENDSEDN"
     gene            7172..8125
                     /locus_tag="PLT63_05310"
     CDS             7172..8125
                     /locus_tag="PLT63_05310"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOD98177.1"
                     /translation="MIQRIIDLIDRYHKFLITAHVKLDGDALGSELALCRLLGNLGKD
                     AVVYNQDPTPRSYRFLPGADDIVHVLPAPVAFDAAFILDCSELERVGDAAPDIGNVRQ
                     LVNIDHHVSNHGFCELRLIDPAASSTGELIFRLLTAMGADIDREMATNLYTAILTDTG
                     GFRYGNTGREALAAAGFLVGRGADPQWISENIYEKCPRAKIMLMARVMQSLSFDCGDR
                     VGSLTVLLRDFEEIGALPEDTEGLVDLPRTIDGVSVSVLFTEIGEQRFKVSLRSKGTT
                     DVAQVAAVFGGGGHVNAAACRIAGDIAVVKRRLFAAMRLPE"
     gene            8122..9066
                     /gene="truB"
                     /locus_tag="PLT63_05315"
     CDS             8122..9066
                     /gene="truB"
                     /locus_tag="PLT63_05315"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOD98178.1"
                     /translation="MMMHGVVVIDKPAGRTSHDVVQAVRKILGGVRAGHTGTLDPLAT
                     GVLPVCVGEATKLVPFLIGAAKEYRVSMLLGVRTDTLDIEGRVVERRPPEVAVDAVHA
                     VLKRFVGRSEQVPPRYSAVKVRGQALYKWARRGIEMEAPPRPVEIYALDVEEVSLPYV
                     RFRLTCSAGTYVRSLCGDAGDLLGCGACVAELRRTRSGAFSEAAAVEMGPADRMEERE
                     RLYELLIPLAAALPDLPEITLPEAWRRRIRDGWQPGAEMVNNRDDIPFLAPGDMVKLT
                     GRDGKLTAIARMQYGSPDLPGLPEDVPVMKLLRVFNEN"
     gene            9104..9370
                     /gene="rpsO"
                     /locus_tag="PLT63_05320"
     CDS             9104..9370
                     /gene="rpsO"
                     /locus_tag="PLT63_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOD98179.1"
                     /translation="MLDSERRQGIIAQYKLHEKDTGSPEVQIALLSARIEYLTEHFKV
                     HKKDHHSRRGLLKLVGQRRRLLDYIKKKDIERYRAVISRLGLRK"
     gene            9402..11555
                     /gene="pnp"
                     /locus_tag="PLT63_05325"
     CDS             9402..11555
                     /gene="pnp"
                     /locus_tag="PLT63_05325"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOD98180.1"
                     /translation="MKEIFKGEFAGRTLSIKARYLAEQADGAALVTYGDTMVLVTVVS
                     LKTGREGLDFLPLTVDYQEMTYAAGKIPGGFFKREGRPNEREILTSRMIDRSIRPLIP
                     KGYNFETQIVANVLSLDTDNDPAVAAMIGASAALVMSDVPFKGPVAGVRVGRSAGQLV
                     CNPGAEALKESDFNIFLTGRKITPGTRGRDFDVNLVMLEGEAKEIAEDDLVDAINAGL
                     EAMRPIIELQDQMRRKIGKEKRTIAEATVDEGLTKMVADTVTAGLQEAYRTPRKQERY
                     AMIDALRDKAVKTCAVKTSPDDDSAPVPDPALVSQVLRSFETLDRSILREKILREKRR
                     IDGRSFTDIRPISSEVGLLPRVHGSALFNRGETQALAAMTLGTSSDEQRIDFIGGEER
                     RAFILHYNFPPYSVGEAKRLGSPGRREIGHGNLARRALSAVLPPAETFPYTIRIVSEI
                     LSSNGSSSMATVCGGCLSLMDGGVPIKAPVGGIAMGLLQEGDEVVILSDILGDEDHAG
                     DMDFKVCGTKEGVTAMQMDIKIDNLTEEILRRALGQAREGRIHIIDRMLETLAASRSD
                     ISLYAPRITTVKVKPEKVRDVIGSGGKNIRQIVSETGVTIDVEDDGTVTIASADAEAA
                     ARAVAMVRWLTEEAEIGKIYTGTVKKVVDFGAFVEILPGTEGLVHISQLAKNRVNKVT
                     DVVNEGDEIMVKVLEIDKQGKIRLSRKEALGANVS"
     gene            11545..12801
                     /locus_tag="PLT63_05330"
     CDS             11545..12801
                     /locus_tag="PLT63_05330"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOD98181.1"
                     /translation="MYRKTILDNGLRIISEEIEHVRSISIGIWVECGSRYENEQSNGT
                     AHFIEHMLFKGTERRSAFDIAADIDSVGGVMNAFTSKELTSFYIKIPDYHLELAIDLL
                     ADIFNNSRFDDEEILKEKSVVLQEISMLEDSPDDYIHDFFEANFWRADRLGMPVLGTR
                     ERVERFTRDSLRGFFRDRYRGRRVILTAAGHLRHDRFVALAGRAFGEMDGEAEPIGFT
                     TPTVSANISVLEKDLEQVHLMIGTLAPSSRSERRHAGFLMNAVLGGSMSSRLFQEIRE
                     KRGLAYAVHSYITPYMDTGMLAVYVGTGKEEVPEVLGLILNELMRLTGESLTGRELQG
                     AKELIKGNFLLSMESTDNRMTRLAKNEICFGRHVPPEEVTAAIDAVTDGDVLTLAREL
                     FNPSTISISAIGPVSERDLTLHVLNG"
     gene            12829..13272
                     /gene="dut"
                     /locus_tag="PLT63_05335"
     CDS             12829..13272
                     /gene="dut"
                     /locus_tag="PLT63_05335"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001862.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOD98182.1"
                     /translation="MDEVEIEIVQLPGCSDLPLPRYMTDQAAGMDICAAVAEATTIRP
                     GRRAVIATGLAIALPPGYEAQIRPRSGLAMKHGITLVNAPGTIDADYRGEIKLLLVNL
                     GDEPFTVRRGDRVAQMVIQRVSRGRWRRREELPPTPRGGGGFGHT"
     gene            13401..14570
                     /locus_tag="PLT63_05340"
     CDS             13401..14570
                     /locus_tag="PLT63_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HOD98183.1"
                     /translation="MKKNRVSKTYQEINEKIRQGKAVVLTAEEMIDVVERHGEVEAAR
                     RVDVVTTGTFSPMCSSGAFLNFGHTSPKIKAARVWLNDVPAYGGLAAVDCYLGATEVA
                     EGDPLNSVYPGEFNYGGGHVIEDLVAGNVVRLRLEGYGTDCYPARKQERNMTLSDMRD
                     AILFNPRNAYQNYSCAVNVSDKTIYTYMGMLRPRLANANYATSGQMSPLFNDPYYRTI
                     GLGTRIFLGGAQGYVAWHGTQHNPHVQRGKNGVPREGAGTLATIGNLKEMSPRWLVGA
                     SMLGYGVSLYVGIGIPIPILNEEMARFTAVRDDDLYVQIYDYSMDYPKGDTKSLGEVS
                     YGDLRTGEIVLDGKKISTAPLSSYYKAREIAMILKEWIEQGDFLLGEPQHLLPSV"
     gene            14698..>14906
                     /locus_tag="PLT63_05345"
     CDS             14698..>14906
                     /locus_tag="PLT63_05345"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOD98184.1"
                     /translation="MALFISAMMVFSNRGLIDNYVMREQLESLKRANQEIDNENRELR
                     KVVYCLRTDLHYIEMVARNDLEMVK"
BASE COUNT         3201 a         4008 c         4728 g         2869 t
ORIGIN      
        1 ggccgctttg gcatactccc ggtaattgcc ggcgatcttg agatccgtgg aggtcatcat
       61 cagggccgtg gttcccagga gaaacagcac ggtgattatg gccagagcca ccggcagggc
      121 gatgccgttt ttccctctcc atattccctg ccgccggttc tcttctctcg tccgttgatg
      181 cgataatggg ttcataatct tgaaaagcct catattttta cgggggcatc atgtttagcc
      241 gtgcccgcgc cgggcgcggg gttgttccct catgccccct attatgtgga cacgacgccg
      301 gtcgcccctg tattggccgc cgtggtgttg taggcgccca gatttctcgg taccacaaag
      361 gattccaact ggtagcgccg atagtgatcg ccggcggtcg ggtgggtgaa atagggatcc
      421 ggctgggcgg tacgggtggt gatgcttatc cttatcttcc ggatggcggt cagatcggtc
      481 gtgatattac cctctccgtc gtagtaggcg aaggacaggg cgtccacgta ttccaccacg
      541 ggcagcttga caccgttgct ggtgcggttg agggccggca cgccgctgga ggaatcgacg
      601 ttgaaggcga tgttttcgtc cttgttggcc gttccagccg tggtatcgca gttggcgttg
      661 agatccgcca caaaacggat ttcgttgccg ccggcatagg tgatcccggt gcagggggta
      721 ttggctgccg tggtcgttcc tgtgcagcgc cggacagccg tcaccggatt cgggcaggcg
      781 gcggaggtct gattgaaacc ggccatgctg atctcccgga tcatcatcgc catggccatc
      841 cgggcgttct gctgcatctc ggtgatctgt ccctggttgc ggtactgccg gttctgcatg
      901 atgaacaggg aatagaccgc gcctacgacc accaggccga tgagcatggc gatgagcaac
      961 tccggcacgg aaaaaccatc gtttcttttc aacatccctc agtccctcgt ccggatggtc
     1021 ttcaattcca cggtgctggg tttcccctgc caggtccagt tgactgtaac cgtgatgatt
     1081 ttgtagtcgt ggggggaagg cgcgtattca agactccaac tcctggcata gatgccgctg
     1141 gtgtccgccc cgccggtgag accggcatag ctcttccctt tcagctcttc gatcttgtct
     1201 ctggccagcg tcgttgccga ggtcatggtc ttgctgaaat cgctggtttg ggtgttcgtg
     1261 gaggtcatgg ttgccagccc gattatcgcc acggccacca gcaggatggc gatgatgatc
     1321 tcgatgatgg aaaacccccg ctgccttttc tgcgcttggg taggtaaacc cttcatggat
     1381 ccgtctcgtt tcctaaagaa attaatgtat atattcgtta ggttaaagaa tatctctccg
     1441 gaagcaggag tataaaacat ctcgcctaag ggcaactggc ggtttcctcg gtccttaccc
     1501 gcccgctgct gttgaggctc agccggttcg gcttcagccc cggggcccca tggatacaga
     1561 tctttcccgt acttgcggtc ccatcggggt agaaggtcaa gaccgcacct gtggtactgg
     1621 taaatgttac atcataatat gtggggtgga tatccttgac ggagaccgcc tccacggaat
     1681 ccaacacccc gtttttgttt acgtcccgga agatggtgaa ctgatgattg ctcccgatgt
     1741 tcatggcaat ccaccggttc tccgtcaccg cttgcatccg catggccatg agactgtttt
     1801 gcagttccct gaccgccccg ttgagtcgtc gctgggccag gtaatggcgg aaggtgggaa
     1861 tggaaatcgc ccccaaaatc gcgaggatgg caatggcaat aagcaattcc accaggctga
     1921 agccccggct gttggatctt tcgatgatca aaattcccct tttcccttcc gccccgcggg
     1981 caccttgaac agccgttgcg ggaagccgga acctctgtcc cggacttatt gtttatataa
     2041 aaatatcata atgtccatga caagacaagg aaatcaaagg cgttgatttt tgcttgcatt
     2101 tgcctctcat ttatgttagc gagctttcaa catcatcgtt gcgttgctga tgagtgggcg
     2161 gttgcccact ttttttttcc cacgggatta tgagtttgat cagaaaagag atggaagagc
     2221 ggatctggac aattaccgag ccggcgatcg tcggagagga aatggagctt gtgtgcgtgg
     2281 aatgcctccg gatgaaatcc cgctggctcg tcaggctcta tatcgacaaa gagggtggtg
     2341 tcaccatcga cgattgcgcc ctgatcagcg aggaggtggg agatctcctg gcggtccacg
     2401 atgtgccgca cgaggcctac accctggaga tctcttctcc cggcctggat cgccccctgg
     2461 taaaggaccg ggatttcctc cgttttcagg gaagcaaggt ggcggtgcag acggagcata
     2521 aaattgacgg agcgaggaat ttcaagggta ttctgcaaga gtaccgcata ggcgaggacg
     2581 gggggaagac cctcgttatc gagagagaag ggaaactcat tgccatcccc agagaagcgg
     2641 tggccagggc ccgtctcgaa tctgtttgga atgattgagt aagttagaaa aatcatcatc
     2701 accgatgatc acatggagga agatggcatg ttgtcggagc tgaaacggct gatcgaacag
     2761 atgtgcaagg accggggact cgacaggacn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnng aattcaagga ccgcaagctg cagctcatca acggttttgt ccagaggttt
     2941 gaagggggca gcatcatcgt caatctgggc cgggcggagg gtgtgatccc ggtttcggaa
     3001 cagatctacc gggaggtgta caagcgcggg gaaaggatca gatcgttcat ctgcgatgtg
     3061 aaaaggatta ccaaggggcc gcagatcatc ctctcccgca ctcatccggg tttcttgaag
     3121 gccctgttcg aggtggaggt gccggagatc tccgaagggc tcattgagat cgtcagcgtg
     3181 gcaagggaac cgggaaaacg ggcgaagatt gccgtcagga gcaaggataa ggacatcgat
     3241 ccggtggggg cctgcgtcgg catgcggggt tcccgggttc agagcgtcgt ccaggaattg
     3301 cggggagaga agatcgatat cgtcccctac tccgaggatc cggccaaata cgtatatgcc
     3361 gccctggccc ccgccaaggt ggatcgggtt ctgatcgatg gggagaaccg gagcatggaa
     3421 gtcatcgttc ccgacgacca gctttctctg gccatcggga agaatggtca gaacgtccgt
     3481 ctggccgtaa agttgacggg ttggaagatc gatgtcaaga gcgagtcttc ctccgcccaa
     3541 caggaagagg acagccgcca ggagctcaaa gcccttcccg acattacgga acagcaaata
     3601 gacggcctca tcaaggaagg gataaagagt ctggccctgg ttgccgccgt ggatgccgac
     3661 gccattatgg tcgtccccgg agtgatcaga gaacaggcgg aggggtggat caaggcagcg
     3721 acggagatga ccgaggaagc ggcggcggcg gaggttagga aatagatcat cgccgccacg
     3781 ttatcaggag catgaacagg ctcggaatat gaggacgcag tagcgggaga accactatgt
     3841 cgaagaaaag agtctacgaa ctggccaagg aattgggcgt ggagaataag gaacttattg
     3901 cccgtctgga aaaaatgggg atatccgtca aaacccactc cagcaccctc gaggacagcg
     3961 aggtagagcg ggttaaaaag gagtatctgg cggccgaaac tcacgagatg gtcgagaaaa
     4021 gggtcaaggc cacggtcatc agacgccggg cggttcgccc cgttgtcgag gaggaggcct
     4081 cggaggcgga accgtcggcc ggagaagcgg cgccgcccgc ggaaccgccg gtcgcggcgg
     4141 gtgaggaaaa gccgggggaa agtgaagccc tcccggcggc ggaatccccg gctgccccgg
     4201 ttgcgccccc tcccgcgccg cctgtggcgg cggaaatacc ccccgtgaag gcggaacctc
     4261 ccgaaacggc cccggttgcc ccacccattt cggaggcccc ggcggtcgca gctcccgttt
     4321 cgcccatggc ccctcccccg tcggcacccc ggccccccaa aacggaaaag aaggacggga
     4381 agcttttgat catgccccgc aagcctcaga atacggctga aatcattaga aaaccgctgg
     4441 ttaagaagat cgaggccgag ccgggcaagg cgccggcggc ccccggcgcc ttgaagaagc
     4501 ccgtgaagtg ggagccggaa aaggacaaga aaaaagggaa aaaacccgta gaggtagtca
     4561 tcggcgacca gttccccgcc cgcaagaagg tcttgatcaa aaaggtcgtg gacaaaaagg
     4621 tacggatcga agatctggag cgggatgacc ggccggtccg gtggcgggag gagaaaaaag
     4681 ccggagcggc gaagctcaag aagacggaga taacgacgcc caaggccatc aagcggcgca
     4741 tcaagatcgc cgagaccatc accgtcggag acctggccaa gaagatgggg gtcaaagcgg
     4801 gagaagtgat caacaaactc atgggactgg gtttgatggt gacgatcaac cagtccctcg
     4861 atttcgacac ggcgacgctt atcgccggtg agttcggtta tcaggtggaa tccgccggca
     4921 tggattttga ggaaaccttc ggaacgaccg acacgggaga acggaacctc aagccccggg
     4981 cgccggtcgt aacgatcatg gggcatgtgg atcacggcaa gacgtcactc ctcgacgcca
     5041 tccgggaaac gaatgtcatc gccggggagg cgggggggat aacccaggcc atcggcgcct
     5101 atcatgtcca actcaaggac cgggacatcg tcttcctcga cacgcccggc catgaggcct
     5161 tcacggccat gcgggcccgg ggtgctcagg tgacggacgt ggttgtcctg gtcgtggcgg
     5221 cggatgacgg ggtgatgggc cagaccatcg aagccatcaa ccattcccgg gttgccggcg
     5281 tcccgatcat tgtggccgtc aacaagatgg acaagccggg ggcggatccc cagcggatca
     5341 aacaggccct gacggaatac ggcctcgtct ccgaggagtg ggggggcgag acaatcttct
     5401 gcgaggtgtc cgccaagcag cgggtgggga tcgaagccct cctggagatg atcctcctcc
     5461 aggccgacat cctggaactc caggcggatc cggaccggcc ggcccggggc atcatcatcg
     5521 aatcgaagct cgacaagggg cgggggcccg tggccacggt cctcatccag gaggggaccc
     5581 tccgggaggg cgacaccttc gcctcgaaga cggaatacgg ccgggtccgg gccatgatcg
     5641 acgatcaggg ccggcgggta aaggaagccg gtccttccat gccggtggag gtgatcggtt
     5701 tttccagcgt cccccaggta agcgccgagt tcctctgcgt ggaggatgaa aagaaggcgc
     5761 ggacgatcgc cgattactgg acccgcaagg aaagggaaaa ggagctgtcc gccacctcga
     5821 agatcaccct ggaacagctc tatcagaaga tcaaggaagg ggtgaaggat ttcaacgtca
     5881 tcgtcaaggc ggacgtccag ggatccatgg aggccatcac cgaggccttg aacaaactga
     5941 gcaccaccga cgtgaaactc cggatcattc atagctccac gggggccatt acggagacgg
     6001 atgtcatgct ggcctcttcc gccaatgcca tcatcatcgg tttcaacgtc cggccggaca
     6061 gccgcgtggt ggaagtggcg gaacaggaag gcgtggaaat caaactctac gacatcatct
     6121 acaacctcct cgaggacgtc cgggcggcca tggaagggct gttggagccg gtttacaagg
     6181 aggtcgtcca gggtcgggcc gaggttcgcc aactgttcaa gattccaaaa gtaggaacga
     6241 ttgcggggtg ttacgttacg gacggcaaga tttcccgggc cgccaatctc aagctggtac
     6301 gggaaggagt ggttgtttac gacggtcgga tcgcctcgct gaaaagacag aaggacgacg
     6361 cccgggaagt ggcaaccggt tatgagtgcg gcatcggcat cgagaacttc aacgaccttc
     6421 acgaggggga tgtgatcgag gcctatatca acgaacaagt ggatcgcaaa ctataggcat
     6481 ttatgatcgt gggcgtggga atggtagaac tgtggctgcc ggagtgccgg tctctgaagg
     6541 agaagcgctc cgtcctgacg aagcttataa aacggactca gaacgccttc aacgtatcca
     6601 ttgccgaggt cggggaaaac gattcctgga aaaaggcccg ggtggggttt tgcgtggtgg
     6661 gaaacgaccg gcggtacatc aacgccaaga tagatcacat cttcaactat atggaaggta
     6721 tggagatcgc ccaggtgatc ggtacgaatc tggagataac cagttattcg gagtggatgg
     6781 caggaacatc cgggccgggg gaaacagcgg aaaaatatga cgacttttaa acgggcccag
     6841 cgggtggccg aacggatcat ggccgagctg tccgagatcc tggtccggga agtgagcgat
     6901 ccgcggatga agagccttac cataaccggg gttaaggtaa ccgacgatct gcggttggcg
     6961 cggatctatt tcgtggagat gggacaggac gtctgccgcc ccgagaccag gcaggccctg
     7021 cagaaggcga atggttttct gcgccgggaa ttggggaaac ggctggaact ccgttacgtt
     7081 cccgaagtcg tcttttcttt cgatccgtcc tttgcctacg gaagccgcat cgaaaagata
     7141 ttggccggga ttcgtgccgg acgggaggaa aatgattcag aggataattg acctgatcga
     7201 tcgttatcat aagtttctca ttacggccca cgtgaagctg gacggagatg ccttgggttc
     7261 ggagctggcg ctttgccggc tgctcggaaa tctcggcaag gacgcggttg tttacaatca
     7321 ggacccgacg ccccgtagct accgcttcct tcccggcgcc gatgacatcg tccatgtcct
     7381 gccggcgccg gtggccttcg acgccgcctt catcctcgat tgcagcgaac tggaacgggt
     7441 gggcgacgcc gccccggaca tcggcaacgt tcggcaactg gtcaatatcg atcaccatgt
     7501 ttccaaccac ggcttttgcg agcttcggct catcgatccg gcggcaagtt cgacgggaga
     7561 gctgattttc cgtctcctca ccgcgatggg ggcggacatc gaccgggaaa tggcgaccaa
     7621 cctctatacg gccattctca ccgatacggg cggtttccgc tacggtaata caggtcggga
     7681 ggcattggcg gcggcagggt ttctcgttgg ccggggcgcc gatccccagt ggatatcgga
     7741 aaatatctat gaaaaatgtc cccgggccaa aataatgctc atggcccggg tgatgcaaag
     7801 cctcagcttc gattgcgggg accgggtcgg ctcgctgacg gtcctgctcc gtgatttcga
     7861 ggaaatcggc gcccttcccg aggatacgga agggctggtg gatctgcccc ggaccatcga
     7921 cggcgtctcc gtatccgttc tcttcacgga gataggggag cagcgcttca aggtcagcct
     7981 tcgttccaag gggaccacgg atgtggccca agttgccgcc gttttcggcg gcggcgggca
     8041 tgtcaatgcg gcggcctgcc ggatcgcggg ggatatcgcc gtggtgaagc ggcggctttt
     8101 tgccgccatg cgcctgccgg aatgatgatg cacggggtgg tcgttatcga caagcccgcg
     8161 ggccggactt cccatgacgt ggtccaggcg gtgaggaaga tcctgggcgg cgtccgggcg
     8221 ggtcatacgg gtactctgga tccgctcgcc acgggggtgc tgcccgtgtg cgtcggcgag
     8281 gcgaccaaac tcgtcccctt tctcatcggg gcggccaagg aataccgggt gtccatgctc
     8341 ctgggggtcc gcacggatac cctggatatc gagggacggg tggtggaacg gcgaccgccg
     8401 gaggttgcgg tcgatgccgt tcatgcggtt ttaaaacgct ttgtggggcg aagcgagcag
     8461 gtgccccccc gctattcggc ggtgaaggtg cggggccagg ccctgtacaa atgggcgcgc
     8521 cggggaatcg agatggaagc gccgccgcgt ccggtggaga tttacgcact ggacgtcgag
     8581 gaagtgtccc tgccttatgt gcggtttcgc cttacctgtt cggcgggcac gtatgtccgt
     8641 tcgctctgtg gcgacgccgg tgatctcctg ggctgcgggg cctgtgtggc ggagttgcgg
     8701 cgtacccgga gcggcgcctt ttctgaagcg gcggcagtgg agatggggcc ggcggaccga
     8761 atggaagagc gcgagcggct ttacgagctc ctcatccccc tggcggcggc gctgccggac
     8821 ctgccggaga ttaccctgcc ggaggcctgg cggcggagga tccgggacgg ttggcagccc
     8881 ggtgcggaaa tggtgaataa tcgtgatgat attccttttc ttgctccggg agatatggta
     8941 aagctgaccg gcagggatgg taaactgacg gcgatcgccc ggatgcaata cggcagtccg
     9001 gacctgccgg gcttgcccga agatgtcccc gtgatgaaac tgctgcgagt ttttaacgaa
     9061 aattgaatga atcgatataa ccagtaaaga gggaggaaca aaagtgctag attcagaaag
     9121 aaggcaagga atcattgccc agtacaaact gcatgaaaag gataccggtt ctccggaggt
     9181 ccagattgcc ctgctcagtg cgcggatcga gtatctcacc gagcatttca aggtccacaa
     9241 aaaggaccac cattcccggc ggggcctgtt gaagctggtc ggtcagagga ggcgcctgct
     9301 ggattacata aagaaaaagg acatcgaacg ttaccgggcc gtgatctcac ggctgggact
     9361 gagaaaatag cgaaagcaat tagagaggat atgatatttt gatgaaagaa atattcaagg
     9421 gcgaatttgc gggtagaact ctgtctataa aggcccgtta tttggcggaa caggccgatg
     9481 gggccgcgct ggtaacctat ggagatacga tggtcctggt gacggtcgtc tccctgaaaa
     9541 ccgggagaga ggggctcgat tttctgcccc tgaccgtcga ttaccaggaa atgacctatg
     9601 ccgcggggaa gatccccggc ggcttcttca aacgggaggg gagaccaaac gagcgggaaa
     9661 tcctgacctc ccgcatgatc gatcgctcga tccgcccgct catccccaaa ggttataatt
     9721 ttgagaccca gatcgtggcc aacgtccttt ccttggatac ggacaacgat ccggcggtgg
     9781 cggccatgat cggcgcctcg gcggccctgg tgatgtccga tgtccccttc aagggaccgg
     9841 tggccggagt ccgggtggga agaagcgccg ggcaactggt ctgcaacccc ggggccgagg
     9901 ccctgaagga gagcgatttc aacatctttc tcacggggcg aaagattacc cccgggaccc
     9961 ggggccgtga tttcgacgtc aacctggtca tgctggaggg agaggcgaag gagatcgccg
    10021 aagacgatct ggtggacgcc atcaatgccg gcctggaggc catgcgtccc atcatcgaac
    10081 tccaggatca aatgcgccgg aaaatcggga aggaaaagag aaccatagcg gaagcgacag
    10141 tggacgaggg attgacgaaa atggtggccg atacggtaac cgcgggcctg caggaggctt
    10201 accgaacccc gcggaaacag gaacgttatg ccatgatcga tgcgctccgg gataaggccg
    10261 tgaagacctg tgcggtgaag acctcccctg atgacgattc cgccccggtt cccgatccgg
    10321 ctctggtttc ccaggtttta cgcagcttcg aaaccctgga ccgcagtatc ctccgcgaga
    10381 agatcctccg ggaaaaacgg cgcatcgacg gtcgttcctt cacggacatc cggcctatat
    10441 catcggaggt cggcctgttg cccagagtcc atggctccgc ccttttcaac cggggcgaga
    10501 cccaggccct ggcggccatg accctgggga cttcttccga tgagcagcgg atcgatttca
    10561 tcggcggcga ggaacggcgg gccttcatcc tccactacaa tttccctccc tactcggtgg
    10621 gcgaggcgaa aaggctcggc agccccggcc ggcgggagat cggacacggc aacctggccc
    10681 gccgggcatt gtctgcggtg ctgccgccgg cggagacatt tccctatacg atccgcatcg
    10741 tttccgaaat cctgtcctcc aacggctcat cctccatggc gacggtctgc ggcggctgtc
    10801 tgtccctcat ggacggcggg gtgcccatca aggcccccgt gggcggcatc gccatgggtc
    10861 tcctccagga gggagacgag gtggtcattc tatccgacat attgggggat gaagaccatg
    10921 ccggcgatat ggacttcaag gtctgcggca ccaaggaagg cgtaacggcg atgcagatgg
    10981 acatcaagat cgacaacctc acggaggaga tcctgagacg ggcgctcggg caggcccggg
    11041 aggggagaat ccatatcatc gatcggatgc tcgagacgct ggccgcgtcc cggtccgaca
    11101 tctctcttta tgcgccccgg atcaccaccg tcaaggtgaa gccggagaag gttcgggacg
    11161 tcatcggcag cggcggcaag aacatccgcc agatcgtcag cgagaccggg gtgaccattg
    11221 atgtggagga tgacggcacg gtcaccatcg cttccgcgga cgcggaggcg gcggcccggg
    11281 ccgtggccat ggtccgctgg cttaccgaag aggcggagat cggcaagatt tacaccggga
    11341 cggtgaaaaa ggtggtggac ttcggcgcct tcgtggagat cctgcccgga acggaaggtc
    11401 tggtacatat ctcccagttg gccaagaacc gggtcaacaa ggtgacggat gtggtcaacg
    11461 agggcgacga aatcatggtc aaggtcctcg agatcgacaa acagggcaag attcgcctga
    11521 gcaggaagga agccttgggg gcgaatgtat cgtaagacga ttctggacaa cggactgcgt
    11581 atcatctcgg aggagatcga gcatgtccgt tccatttcca tcggtatctg ggtggagtgc
    11641 ggttcccgtt acgaaaacga gcagtccaac gggaccgccc attttatcga gcacatgctc
    11701 ttcaagggca cggagcgacg aagcgccttc gacatcgccg cggacatcga ttccgtcggc
    11761 ggcgtcatga acgcctttac cagcaaggag ctgacgtcat tttatatcaa gattccggat
    11821 taccatctgg agctggcgat cgatttactg gccgatatct tcaacaattc ccgattcgac
    11881 gatgaggaga tcctcaaaga gaaatcggtg gtcctccagg agatcagcat gctggaggat
    11941 tctcccgacg attacatcca cgacttcttc gaagccaatt tctggcgggc ggaccggctg
    12001 gggatgccgg tcttggggac ccgggagcgg gttgaacgct ttacccggga ttccctgcgg
    12061 gggtttttcc gcgaccgtta tcgaggccgg agggtcatcc taaccgcggc ggggcacttg
    12121 cggcacgacc gtttcgtggc gctggccggc cgggccttcg gggaaatgga cggcgaggcg
    12181 gaaccgatcg gctttacaac gcccaccgtg tccgccaata tttccgtcct ggaaaaggac
    12241 ctcgaacagg tccacctcat gatcgggacc ctcgccccct cgtcgcggag cgaaaggcgt
    12301 catgccggct ttctcatgaa cgccgtcctg ggggggagca tgagttcccg cctctttcag
    12361 gaaatccggg aaaagagggg cctggcttac gccgtacact cctacataac cccgtacatg
    12421 gatacgggga tgctggccgt ctatgtgggg acggggaagg aggaggtccc ggaggtcctg
    12481 ggattgatcc tcaacgagct gatgcgtctg accggagaga gtctgaccgg cagggaactc
    12541 cagggggcga aggagctcat caagggcaat ttcctcctgt ccatggaaag cacggacaac
    12601 cggatgaccc gactggcgaa aaacgaaatc tgtttcggcc gccacgtgcc gcctgaggag
    12661 gtgacggcgg ccatcgacgc cgtcaccgac ggggatgtcc tgaccctggc acgggagctt
    12721 ttcaacccca gcacgatctc catctctgcc atcggcccgg tgtcggagcg ggatctgacg
    12781 ctccatgtcc tgaacggata gacaacgggc aaagggccgc gggcgacagt ggatgaggtt
    12841 gaaatagaga tcgttcagtt gccgggatgc tcggatctgc ccctgccccg atacatgacg
    12901 gatcaggccg ccgggatgga catttgcgcc gccgttgcgg aagcaacgac gattaggccc
    12961 ggtcgccggg cggtgattgc caccgggttg gccatcgccc ttccccctgg ttacgaggcc
    13021 cagatccgac cccggagcgg cctggcgatg aagcacggca tcaccctcgt caatgccccc
    13081 ggcaccatcg acgccgatta ccggggcgag atcaagctcc tgctggtcaa tctcggcgac
    13141 gaacccttta cggtccggcg cggggaccgg gtggcccaga tggtgatcca gcgggtgagc
    13201 cgcggccggt ggcgccgccg ggaagagctg ccgcccacgc cgcgcggtgg cggtggcttc
    13261 ggccatactt gaattgttgc cgttaccgtg gtgagagatt atcggtaaaa ggcaaatggt
    13321 gagggcgtcg agcatcgcta aagcggcgct aaataaaggg gcctgaaccc ggcaacggcg
    13381 tctttttaga ggtttacgct atgaagaaaa acagggtgag caagacctat caggaaatca
    13441 acgagaagat ccgtcagggc aaggcggtgg tgttgaccgc cgaagagatg atcgacgtgg
    13501 tggaacgcca cggcgaggtg gaggcggccc ggcgggtgga tgtggtgacc acggggacct
    13561 tcagccccat gtgctcctcc ggcgccttcc tcaatttcgg tcatacctcc ccgaagatca
    13621 aggcggcccg ggtgtggctc aacgacgttc cggcttacgg cggcctggcg gcggtggact
    13681 gttacctcgg cgcgacggag gtggcggagg gggaccccct gaacagcgtc tatcccggcg
    13741 agttcaacta cggcggcggg catgtgatcg aagatctcgt ggcgggcaac gttgtccggc
    13801 ttcgtctgga aggctacggc acggactgct atcccgcccg gaagcaggag cggaacatga
    13861 ccctttcgga catgcgggac gccatcctct tcaatccccg caatgcctac cagaattaca
    13921 gttgcgccgt aaacgtgtcg gacaagacga tctacaccta catggggatg ctccgtcccc
    13981 gcctggccaa cgccaattat gccacttcgg ggcagatgag ccccctgttc aacgatccgt
    14041 actaccgcac gatcggtctg gggacgcgga tctttctggg aggcgcccag ggatacgtag
    14101 cctggcacgg gacacagcac aatccccatg tccagcgggg gaaaaatggt gtgccccggg
    14161 agggcgccgg caccctggcg accattggca acctcaagga aatgtcgccg cggtggctgg
    14221 tgggggccag catgcttggt tacggggtct cgctgtatgt ggggatcggg atccccatcc
    14281 ccatcctcaa cgaggagatg gcccggttta cggccgtccg ggacgacgat ctctatgtcc
    14341 agatatatga ttacagtatg gattatccca agggagatac caagagcctg ggagaggtaa
    14401 gttatgggga tttgcggacg ggggaaatcg tcctcgacgg taaaaaaatt tccaccgctc
    14461 cgctgtccag ctattacaag gccagagaaa tagcaatgat attgaaggag tggatcgagc
    14521 agggagattt tctcctgggc gagccccagc atcttttgcc ttcggtttga ggggagaaaa
    14581 ataatatttt cttggaacat attattttta ttgcgaagaa aggattttta ttttataata
    14641 agtaaaattt gatcattaaa agcaggcatc atgaaaatcg ggcgttacgt gttagcattg
    14701 gctctattca taagcgccat gatggtgttc agcaaccggg gcctgatcga taactatgta
    14761 atgcgggagc aattggagtc cctgaaaagg gctaaccagg aaatagataa tgaaaacagg
    14821 gagttgcgta aggtagtgta ttgcttgcgg accgatcttc attatataga aatggttgcc
    14881 cgcaacgatc tcgagatggt caagaa
//
LOCUS       DAOSMF010000034        14578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_4853_length_14578_cov_14.966123, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000034 DAOSMF010000000
VERSION     DAOSMF010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 14578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14578
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4853_length_14578_cov_14.966123"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..351)
                     /locus_tag="PLT63_05350"
     CDS             complement(31..351)
                     /locus_tag="PLT63_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98185.1"
                     /translation="MPEYLSNLVCDKTELLKIFRQRSKPFVEKTVKGKNLALAKEKAE
                     IEKKEGWTILPDKYKNSVKLKRPKPADIALEDEVWSLLFKMGFDEYSKDRNFKIFGSV
                     KFFV"
     gene            complement(470..1810)
                     /locus_tag="PLT63_05355"
     CDS             complement(470..1810)
                     /locus_tag="PLT63_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98186.1"
                     /translation="MSKQTISKGKSFKAGMYLLDSFTSGMYNDPLIIYREYIQNAVDS
                     IDMSSDWQDVPFEVTIRLEPGTRRITIRDNAMGVPSALAEEVLSSVGSSNKTLGKMRG
                     FRGIGRLGGIAFSDRAVFRTKAKDEELESIQEWDCQNLRRLLAEQRKDAFTLEDVFLR
                     VTTFRQEQCQNTEESYFEVTLSGVSSFRNQLFDLDRVRRYLSQVAPVPFNPESFSFAQ
                     TIDEYLSGTLSSYCRYNISLNGERIRKPYRDSVKISKGNTDHIEGINFFQIENSKDRP
                     FAHGWYGRREKLLGAINRGDDLSGIRVRVGNIQIGDAHLLDLCFREPRFNGYVIGEIH
                     VDSPRLIPNSRRDDFVDNTNKGAFYNAIEKEIGLPISKEIRFQSRLASNQVSPALVPE
                     QKKKESFISDSTEPVSPRKDKPVIAPPDDIPISLQDVCQDCPNLSSVLAFMKQR"
     gene            complement(1827..3236)
                     /locus_tag="PLT63_05360"
     CDS             complement(1827..3236)
                     /locus_tag="PLT63_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98187.1"
                     /translation="MGSRNILLVEPRYPTKFPPLGLMKLSTYHKALGDRVHFVKGPDK
                     DVPYEYWDRIYITSLFTYHWKITLEDILYYKNMLHGDTSRIYIGGIMASLMPDELWKE
                     TGICPMIGILDKPGLLDGDNDLVVDDMIPDYDLFDKITHRYSLLDSYFGYLTRGCVNK
                     CKFCGVPIIEPNFRDYAGIKPWVKLINDTYGQKHNLVLFDNNIIASKVFTEIIEDIRE
                     LGFERGAKLNNRLRHVDFNQGTDARLLKEWHFKLLSKISIDPLRIAFDHVRQTRIYSA
                     SIRLAAKYGIHNLSNYILYNYDDTPQELWQRLKLNIDFNRQLGVQIYSFPMKYVPLDA
                     KDRTHIFEPHWNWQFIRGVQRILNVTKGIVMPGEEFFYRAFGESEETFLRILHMPERM
                     LMSRGRVPGEEERDWVSKFDNLTNGEKAELLTILCENRTKKNLVGTIRRNRNGQLKHI
                     LDYYLTEPEADNKLTIFDE"
     gene            4026..4808
                     /locus_tag="PLT63_05365"
     CDS             4026..4808
                     /locus_tag="PLT63_05365"
                     /inference="COORDINATES: protein motif:HMM:NF020962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin"
                     /protein_id="HOD98188.1"
                     /translation="MTAAKFISDLVARGRYCFGTAEAVDALGTTVVAARAALRRLRQK
                     GELATPYKGFYVIVSPEFRNAGCLPASHFIPSLMEHMGEKYYAGLLSAAEYHGAAHQR
                     PQVFQVVVARNIPEITCGKVRVIFVARKNMARIPTADFKTPRGYLKISTPEATAFDLV
                     GYPKHAAGLDNVATILAELAENIDGDALVSLAEFSPIAWAQRLAYLLDLVGAQDKTVA
                     LVKYLVEKKPAPTPLAPSQHVDGISSLKPWNVIVNTTVEADI"
     gene            4805..5611
                     /locus_tag="PLT63_05370"
     CDS             4805..5611
                     /locus_tag="PLT63_05370"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOD98189.1"
                     /translation="MIPNDFISEWKQTAPWSHNGQVEQDLIICRALVEIFNHPDLEEN
                     LAFRGGTALFKLHMPPIRYSEDIDLVQVHPGPIGPLMNALQAQLNPWLGNPKRKLSEG
                     RVTLTYRVASEEGLPLKLKVEINSREHFTVLGFEKRIFAVDSRWFSGTTSILTYHLDE
                     LLGTKIRALYQRKKGRDLFDLWTAFVTAKVQPERVIDCFLHYMENEGRRITRAEFERN
                     LLAKIDDPRFIADIEPLVIANSGFDLPWASEFVFNSLVTLIPGEPWQGKA"
     gene            complement(5777..6040)
                     /locus_tag="PLT63_05375"
     CDS             complement(5777..6040)
                     /locus_tag="PLT63_05375"
                     /inference="COORDINATES: protein motif:HMM:TIGR01764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOD98190.1"
                     /translation="MTTRDSLRTGLMEKEFQPQKTGQPIPHPLKRYYRVDEVANYFSV
                     SDRTIYRLIDTGDLKAIRLRDCIRVASEELKRFEALQLDDDMP"
     gene            complement(6037..6546)
                     /locus_tag="PLT63_05380"
     CDS             complement(6037..6546)
                     /locus_tag="PLT63_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98191.1"
                     /translation="MDEYFLFAAIATGPGGTERAGTGGRPLCGLYEECIAPLCPLDRV
                     SLKGVWYPGEEICRSRTHGNLTWIRAQRKISRAEATGYFNLEILNSIRTIRKGISGLD
                     PNVDEKPQLRRWLELHEKKRQKPVSGPLQNVAKPGGQVKNFCRNGVSQHPIRTGTGKP
                     SSGRSEPKK"
     gene            complement(6539..6985)
                     /locus_tag="PLT63_05385"
     CDS             complement(6539..6985)
                     /locus_tag="PLT63_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98192.1"
                     /translation="MDKSAFGEFLTSLCHYYERKEPRKETLNLWFQEVKPLPEEPLDW
                     IREQICQRQEGFPRNLPVIMRELWQRWREEHPQKQAHPDTDANCRDCDGTGLITGYRE
                     SHAYAFRCGRCRQSTLHGIPFTTVETLIAQGYERSAPRQGRQRAYG"
     gene            complement(6955..7977)
                     /locus_tag="PLT63_05390"
     CDS             complement(6955..7977)
                     /locus_tag="PLT63_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98193.1"
                     /translation="MARTRTIKPEFWSDEKLATLSLQARLTYIGLWTLSDDYGVVKGH
                     PAWLRNSIFPYDDIPLAQFESWLLELEGIGRIAPFAADGEKFYFIRNFNRHQIINRPS
                     QTRNPSPPETLTEYSVSPHGACSEPSVSTHGALTDEIETERERERERETTTAASRIAD
                     SPLEQIRTRQEPGADHGQEEKAQKPQTEQAQDAHEPEARQVPDVDKPREQEGLSPPAS
                     DDPPPPNQNAGRHQPKPVARVTSPARKQIDFDYETGRFRHVLSELVAKWQEAYPAVDV
                     LSELHRMEAWAAANPVHRKSNWQRFIVNWLTKSQDRARSSGSGKDPPGTEGSVERWIK
                     APSENS"
     gene            complement(8035..9213)
                     /locus_tag="PLT63_05395"
     CDS             complement(8035..9213)
                     /locus_tag="PLT63_05395"
                     /inference="COORDINATES: protein motif:HMM:NF024870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD98194.1"
                     /translation="MFHKAERKKGKLRLAIAGPAGAGKTYSALLIAFGLGGRIALIDT
                     ERGSGELYDHLGAYDVCGIEAPFEPRKYIEAIKEAEELGYGIILIDSLSHAWVGQGGL
                     LDVHGHIADKTGNSWAAWRQVTPKHNELVDAMLQSKCHIIATMRSKMEYAQVEENGKK
                     HVKKLGMGPIQRDGMEYEFTVFMDLDHNHTAAATKDRTTLFDGRYFVPTVETGKLLLD
                     WLEKSGGESPVFQPQEEAGEQEKHQSQEVPAKNAPAKTDAEVQGKQQGPGDVRGGSAR
                     ENVKKEGAQSLQQGGNGDGLKPLIARLALLDIGKEPYQIYCCRRYEIATLTELTPDQI
                     REQAKLLDSLKRPVRLKEFKSFLEQLVSDHTFNTGAPTHSLPAVSGSATAASGAESLQ
                     "
     gene            complement(9216..9485)
                     /locus_tag="PLT63_05400"
     CDS             complement(9216..9485)
                     /locus_tag="PLT63_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98195.1"
                     /translation="MNKYDLWQVTEAKPSAEAGDSKYAERINQRHVQPENTLRVLCVG
                     RIHKGKRTVVIAFHRKPTPIVPDSPSPIEDESLDHGNDLHHERRE"
     gene            complement(9472..9780)
                     /locus_tag="PLT63_05405"
     CDS             complement(9472..9780)
                     /locus_tag="PLT63_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98196.1"
                     /translation="MKQQPPKDTTQVIREAFYDLALSLECLFLLYGTSDLFAEDVVRC
                     LEEGYTSAMKRVRKLGGPPGSGKIRRQTAVTRFLSRLEREKSDRELSGGERRCVDEQI
                     "
     gene            complement(9876..10802)
                     /locus_tag="PLT63_05410"
     CDS             complement(9876..10802)
                     /locus_tag="PLT63_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98197.1"
                     /translation="MGFSFLRIEEELASERFTSLYRRLQEECPHLVAYPSATALIAFF
                     NDVEKEYAEKDALLSFLITVYRRDGPGRLLSGLFIILFRPAIASLYRLARKSNFRLDE
                     EDFIQDVCTLLLEIIATAPVRPQKVAMQIVGPLRNRVRSLLNREIAGGRLTTFGSATL
                     DGDNPMPIGHGNHKGDVEIEKTWDIAAAPDGNHDDVWDGNYGDEMPSSYPGRMTVLPQ
                     GEQGEQGDQGEENATGEWRRYDLTDAESFLTGLVAMGVIGAEDREIIYETVIKERPFK
                     DLATDPKEYQRLKKRRQRCLAAIRKYLRNIPK"
     gene            complement(10865..11113)
                     /locus_tag="PLT63_05415"
     CDS             complement(10865..11113)
                     /locus_tag="PLT63_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98198.1"
                     /translation="MYECRCPHDGHKLLEMARPPLSRLGVKIRCPCGEKANGRVFSDG
                     HSGIILAVAVCACGRKTVRPVGDLVAVRCRTCKKIITF"
     gene            complement(11411..12442)
                     /locus_tag="PLT63_05420"
     CDS             complement(11411..12442)
                     /locus_tag="PLT63_05420"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOD98199.1"
                     /translation="MVIDLLDDLTLDPHIIRILRERHMERVKEIQADILAELPAFSGA
                     MKKIGEDISLLSELLYKAANEAGQGKEEVWKIDNLSQRLISGFTDIGMILSQVGLWRI
                     NKDYLECQSEVNGEIKQSVSIHCAESLRKLVPRTVAEWLDQRLELQPAVAADSYREAH
                     FLLKEASPKGYRLPEELTVREEQTPAFPPSLVEIIADPKLLADFRRRLAQAENLRELI
                     SSPPELVIDARRRRCFYRGIAFLLTGKAFDMLALLAGSPGKLVTRAEIYDRIWAEGDG
                     DGDDNTRPYDRQITDHKYRISAGIAKAAASAADAGSPARGEMRQLIVSRFGSGYILNL
                     PPEEICILP"
     gene            12756..13034
                     /locus_tag="PLT63_05425"
     CDS             12756..13034
                     /locus_tag="PLT63_05425"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOD98200.1"
                     /translation="MNFELEAKDIEAIAGRVIEKLVPLIRELQKPDRVMDILEVSALL
                     GKSKGQIYQWVNLASHGLSDFPFQKAGRSLRFSEDAIQEWMKKKSLRR"
     gene            13328..14521
                     /locus_tag="PLT63_05430"
     CDS             13328..14521
                     /locus_tag="PLT63_05430"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOD98201.1"
                     /translation="MTGSIQKKGKTYYAVLPINGKRKWFKGGTKRDAERVLAEKLAEV
                     SQGSYREIQKISFKEFATMWLKTHAENNVKPSTLASYKHVINKNLVPRFGFLQMSDIT
                     TGGLQVYVAERRKAVASKSVLNEVVLLKQLFKHAHKWGYARSNPGEYLERPKVIKSEI
                     EILDPAEVKLLLNSTGELYRTSFLTAILTGVRAGELWALQWGDIDWHSKRLCIRRSVW
                     KGDFQSPKSQQSVRKIDLPDSLIPELKKWKVRCPISEKDMMFPTAEGKITDHDNARNR
                     YFNSSLRKAGLRHVSFHSLRHTNASMRILAGQNIKYISTQLGHSSVKITLDTYGHLFN
                     DQDFNRHQVGLLETVFSASVRNPLEKVSPKTEFETDFVAPVRNPLEKPLQNAEKGLAA
                     SANPL"
BASE COUNT         3345 a         3768 c         3739 g         3726 t
ORIGIN      
        1 ctttcttcaa ggaattggcc tatgaatatt ttacacaaaa aacttgacac taccaaatat
       61 cttaaagttt cggtctttgc tatattcatc aaaccccatt ttaaaaagta acgaccatac
      121 ttcatcttct aacgcgatat cagctggttt cggtcttttc agcttgactg aattcttata
      181 tttgtcaggg aggatcgtcc acccctcctt cttttcaatc tctgcttttt ctttggctaa
      241 agctaggttc ttgcctttga cagttttttc tacgaacggt tttgatcgtt ggcgaaatat
      301 ttttaataat tccgtcttgt cgcagactaa gttggacagg tattcaggca tggcagcagc
      361 tccttgaata agagacaccg aaaaaatact aacctaaact gctgaatatt tacttgtggg
      421 agggtattta gcaaaaatac ttattgaata caagcgacaa aagctaaaat caccgctgtt
      481 tcatgaaggc cagaacgctt gaaagattag gacaatcttg gcagacatct tgaagagaga
      541 taggaatatc atccggcggc gcaatcacag gcttgtcttt tcttggcgac accggttcgg
      601 tcgaatcact aatgaatgat tcctttttct tctgctctgg cacaagagcg ggagatacct
      661 ggttggaggc aagccgggat tgaaatcgga tttccttgga aatgggcagt ccgatttctt
      721 tttcgattgc attataaaaa gcacctttgt ttgtattatc gacaaaatca tctcggcggc
      781 tgttaggtat caagcgcgga ctgtcaacgt ggatttctcc tattacatag ccattaaatc
      841 ttggctctcg aaaacagagg tccaaaagat gggcgtctcc tatttgaatg ttcccgaccc
      901 gaacccttat ccctgataaa tcgtctccac gattaatagc accaagaagc ttttcccggc
      961 gtccatacca cccgtgagca aacggcctat ccttggagtt ttcgatctga aagaaattga
     1021 tcccttcgat atgatccgta ttccccttgg atatctttac gctgtctctg tatggctttc
     1081 gaatccgttc tccgttgaga gaaatgttat accgacaata actagagagc gtgccggaca
     1141 aatattcgtc aattgtctgt gcgaaggaga aactttcagg gttaaatggt acgggagcta
     1201 cttggctcag gtatcgccgg actcgatcca agtcgaaaag ttgattacga aagctagata
     1261 cacccgagag ggttacttca aaatagcttt cttcggtatt ttgacactgt tcttgccgaa
     1321 aggtggtaac gcgaagaaac acatcttcaa gagtaaatgc atcttttctt tgttccgcta
     1381 aaagccttct caaattctgg caatcccact cctgaatcga ttccaactct tcatccttag
     1441 ctttagtgcg gaagacggcc cgatcgctaa atgcaattcc gcctaaacgc ccgatgccac
     1501 ggaaccctcg cattttccct aatgtcttgt tactgctgcc tactgagcta agcacttcct
     1561 ctgccaatgc actgggcaca cccatggcat tatctcgaat cgttattcgc cgtgtacccg
     1621 gctcaagtcg aatcgtaact tcaaaaggca catcttgcca gtcagaagac atatcaattg
     1681 aatcaactgc attttggatg tattctcgat agataatcag tggatcattg tacatccctg
     1741 aggtaaagct atccaacaga tacattcccg ccttaaaaga tttgcccttg ctgattgttt
     1801 gtttgctcat ccagaccaga cctttcttat tcgtcaaaaa tcgtcaactt gttatcggct
     1861 tcaggttcag ttaaataata gtcgaggata tgcttgagct gtccattcct attccgccta
     1921 atagtcccca ctaagttctt ctttgtccta ttttcacaga ggatagtgag caattctgct
     1981 ttctccccat tggtcaagtt atcgaattta ctgacccagt cgcgctcttc ttcccctggt
     2041 accctcccac gactcatgag cattcgttca ggcatgtgga gaattcgcag aaatgtttct
     2101 tcgctttctc caaaggctct atagaaaaat tcttctcctg gcataacgat gcccttggtc
     2161 acattcaaga ttctttgcac tcctctgatg aattgccaat tccaatgagg ctcgaagata
     2221 tgtgttctat ccttggcatc aaggggaacg tatttcattg ggaaagaata aatctgaacc
     2281 cctaactgcc tattaaaatc aatattcaat ttaaggcgtt gccataattc ttgaggagtg
     2341 tcgtcatagt tatacaggat ataattcgaa aggttatgaa tgccatattt agcagccaag
     2401 cgaatcgaag cagagtatat gcgagtctgc cgtacatggt cgaatgcaat acgcaggggg
     2461 tcaatggata ttttcgaaag aagcttgaaa tgccactctt tcagaagccg tgcatcggtt
     2521 ccttgattaa aatcaacatg acggagcctg ttattgagct tggccccgcg ctcgaatccc
     2581 aactctctta tatcttcaat gatctccgta aacacttttg aggcaataat gttgttatcg
     2641 aagagaacta ggttgtgctt ttgtccatat gtatcattga ttagcttaac ccatggttta
     2701 attcccgcat agtctcgaaa attcggctct attattggaa cgccacaaaa tttgcattta
     2761 ttgacgcacc ctctagtaag gtatccgaaa tagctatcga gcagtgaata tcgatgtgtg
     2821 attttatcaa agaggtcgta gtctggaatc atgtcatcta cgacgaggtc gttgtcacca
     2881 tccagcagtc ctggtttatc caggattcct atcatcggac agatgccggt ttctttccac
     2941 agttcatcgg gcattaggct agccatgata ccgccgatgt agatacgtga tgtatctcca
     3001 tgaagcatgt tcttataata gagtatgtct tcgagggtaa ttttccaatg ataggtaaaa
     3061 agactggtta tgtaaattct gtcccaatac tcgtaaggaa catctttatc gggtcccttt
     3121 acgaaatgga ccctgtcacc caatgctttg tgatatgtgg aaagcttcat cagccccaat
     3181 ggcgggaact ttgtaggata acgtggctcg accagaagaa tatttcttga ccccatacta
     3241 tccaatctct aatttggttg gggctacata tcacagcgcc cggctaatgt ctatctaaat
     3301 ttcaccatgg cctcttcttg gatactgcac gaatctcaat ttttaaaaaa tgaagatttg
     3361 ccttcttcag cgatatataa acaacataaa gttttataat tattgagttt aatttatatt
     3421 tgtcctcgaa agtgactttt attcagcagt aataaattga tggttattgc gccggtgtgg
     3481 tgtgtggttg attgcattaa gattctaaga acatattgac attatctatc tttttgttag
     3541 tgctcaacaa taatcacatt ttaaaggtgg cgttcttttt cttgatttat ttgtttttta
     3601 ctaattatat actcatcttt aatatttaca aataatatgt ttaacgatgg aattgggatt
     3661 gaaattccct tagaacctgg gtcgggtgca tagtctaaca ttattatatt tgcattaaga
     3721 tatttatagc ataatattct aacatttcat acattttctt agaaaaattt cagaaggctt
     3781 tcagattttc ttaaagggaa gagaaagctt tataattttt ttcatccagc attcaatgtc
     3841 tgctccgaaa aaccaaaccc tcgttttgat ttgctatacg ttgatctctg cgataaaaat
     3901 tatatatagt gagaccgtat caataatatt tctaaaagcc accaggcttg gcattgcaag
     3961 tatcactcga cgaaagtaac gaaatgtgtt actttgcctg agcaattcat ttaaaaggag
     4021 acatcgtgac tgcggcgaaa ttcatatcgg atctggtcgc aagggggcga tactgcttcg
     4081 gcactgcgga ggcagtggac gccttgggaa ccactgtggt tgccgccagg gcagcgcttc
     4141 gccgcctgcg tcaaaaaggc gaactcgcta cgccctacaa gggtttctat gtcattgtct
     4201 cacctgagtt taggaatgcg gggtgcctgc cggccagcca ctttataccg tccctgatgg
     4261 aacatatggg agaaaagtat tatgcaggtc tgctcagcgc cgccgaatat cacggcgcgg
     4321 cgcaccagcg cccccaagtc tttcaggtcg tggtagctcg gaacatacct gaaatcactt
     4381 gcggtaaggt ccgggtgata tttgtggcca gaaaaaatat ggcgcgcatc cccactgcgg
     4441 atttcaagac cccgcgcggg tacctgaaga tctccacccc ggaggcaacc gcctttgatt
     4501 tggtcggata tccaaagcat gcggcagggc tcgacaatgt ggctaccatt cttgcggagc
     4561 ttgccgagaa tatcgatggg gatgcattag tcagcctggc ggagttttcg ccaatcgctt
     4621 gggcacagag gcttgcctat ctgctcgatc tagtgggcgc ccaagacaaa accgtggcct
     4681 tggtaaaata tcttgttgag aagaaaccgg cgcctacccc gcttgcacca tcgcagcatg
     4741 tcgatgggat cagcagcctg aaaccgtgga acgttatagt caataccact gtggaggcgg
     4801 acatttgatc cccaatgact ttatctccga gtggaagcag accgcccctt ggtcccataa
     4861 tggtcaggtt gaacaggatc tgattatctg ccgtgccctt gtagaaatct ttaatcatcc
     4921 tgatcttgag gaaaaccttg cctttcgagg cggaacagcg cttttcaagc tccatatgcc
     4981 accgattcgc tattccgagg atattgacct ggttcaggtt catccaggac caataggtcc
     5041 tcttatgaat gccctccagg cgcagctcaa tccctggttg gggaacccga agcggaaact
     5101 ctcagaagga cgggtgacgc ttacctaccg ggtagcttcc gaggaaggtt tgccattgaa
     5161 gctcaaggtg gagatcaact cccgagagca tttcacggtt ctgggatttg aaaagcgcat
     5221 ctttgcggta gattcaaggt ggttttccgg aaccacgtcc atccttacct atcaccttga
     5281 tgagttattg ggaacaaaga tacgggcatt gtatcaacga aaaaaagggc gtgatctgtt
     5341 cgacctgtgg acagcctttg tcactgccaa ggtgcagccc gagcgggtga tcgactgctt
     5401 cctgcattac atggaaaacg aaggccgccg gatcacgcgc gccgaatttg agcgaaatct
     5461 gcttgcaaag atcgacgatc ccagattcat cgccgatatc gaaccactcg tgattgccaa
     5521 cagtggtttt gatctcccct gggcgtctga atttgtgttc aacagcttgg tgactctgat
     5581 accaggggaa ccctggcaag gcaaagctta gatttgacgt tcagcgacag aagccgcgta
     5641 tttcttatga gttgaacgca gtaatgtcaa gtaagtgcta ttgtcatagg gctccttatt
     5701 caagaagact ggaataacgc atcctcagag cacagcttat tcaagacaat tcaaatagcc
     5761 atacgaaaga gttgtatcag ggcatatcat cgtccaactg gagtgcttca aaccttttca
     5821 gttcttcgga ggccacccgg atgcagtccc ggaggcggat cgccttgagg tcgccggtat
     5881 cgatgaggcg gtagatcgtc cggtcggaga cggagaaata gttggccacc tcgtccaccc
     5941 ggtagtagcg cttcaggggg tgagggattg gctgccctgt tttttgtggt tgaaattcct
     6001 tttccatcaa cccggttctc agactgtccc tggtggtcat tttttcggtt ccgacctccc
     6061 ggatgaaggt ttacccgtcc ccgtccggat ggggtgctgg gacaccccgt ttcggcagaa
     6121 atttttcact tgaccccccg gtttcgccac gttttggaga gggcctgaaa ccggcttttg
     6181 ccgcttcttc tcgtggagtt ccagccaccg ccggagctgc ggtttttcat cgacgttcgg
     6241 gtccaggccg gagatcccct tccggatggt gcggatactg ttcaggatct ccaggttgaa
     6301 gtatcccgtc gcctccgccc tgctgatctt ccgctgagcc ctgatccagg tcagattgcc
     6361 gtgggtccgg gaacggcaga tctcctcacc gggataccag acgcccttga gactgacccg
     6421 atccaggggg caaagcggcg cgatgcattc ttcatagaga ccgcataatg ggcgacctcc
     6481 cgtaccggct cgctccgttc cccctgggcc ggtggcaatg gcggcaaaga gaaaatattc
     6541 atccataggc gcgttgcctt ccctgccgtg gtgccgagcg ctcatacccc tgggcgataa
     6601 gcgtctcgac ggtcgtgaag gggatgccgt ggagcgtcga ttgccggcac cggccgcacc
     6661 ggaaggcgta ggcgtggctc tcccgatagc ccgtgatcag cccggtcccg tcgcagtccc
     6721 gacagtttgc atccgtgtcg ggatgggcct gcttctgcgg gtgttcctcc cgccaccgct
     6781 gccagagctc ccgcatgatc accgggagat tccggggaaa cccctcctgc cgctggcaga
     6841 tctgctcccg gatccagtcc aggggctcct ccggaagagg cttcacctcc tggaaccaga
     6901 ggttgagggt ctctttgcga ggctccttcc gctcgtagta gtgacatagg ctcgtcagga
     6961 attctccgaa ggcgctttta tccatcgctc cacgctccct tccgttccgg ggggatcttt
     7021 cccgctcccg ctgctgcggg cccggtcctg tgatttggtc aaccagttga cgataaaacg
     7081 ctgccagttc gatttgcgat gcaccgggtt tgccgctgcc caggcctcca tgcggtgcag
     7141 ttctgagagc acgtcaaccg cgggataggc ctcttgccac ttcgcgacca gttctgagag
     7201 cacatgccgg aagcggccgg tttcataatc gaaatcgatt tgtttccttg cgggactcgt
     7261 tacccgcgcc acaggtttag gctggtggcg accagcattt tggtttgggg gaggaggatc
     7321 gtcgcttgcc ggcggggaca atccttcctg ctcccgaggt ttatccacat ccgggacctg
     7381 acgcgcttca ggctcatggg catcctgcgc ctgctctgtc tgaggctttt gcgctttctc
     7441 ttcctggccg tgatcagctc ccggttcctg ccgcgtccga atctgctcca gcggtgagtc
     7501 ggcaatccgg ctcgccgcag tagtagtttc tctttctctt tctctttccc tttctgtttc
     7561 tatttcatca gtgagggctc cgtgagtact cactgagggc tcactgcatg ctccatgagg
     7621 gctcactgag tactcagtga gtgtctcggg agggctcgga tttcgtgtct gggaaggacg
     7681 gttgatgatc tggtgtctgt tgaaattcct gatgaaatag aatttctccc cgtcggcggc
     7741 gaagggggct atccgcccga tgccctccag ttccaggagc cacgactcga attgggcgag
     7801 gggaatgtcg tcatagggga agatggagtt ccggagccag gcgggatgtc ccttgacgac
     7861 cccgtagtcg tcggacaggg tccacaggcc gatataggtg agccgcgcct ggagggacaa
     7921 cgtcgcgagt ttctcgtcgc tccagaattc cggcttgatg gttctcgtcc tggccatggt
     7981 caattcacat cccgtcatag taatggggca ggaaggctcc tgccccgttt agttttattg
     8041 aagtgattct gcccccgacg ctgccgttgc cgatcctgaa actgcgggaa gggaatgggt
     8101 cggggctccc gtgttaaatg tgtgatccga gacaagctgc tcaaggaatg acttgaactc
     8161 cttcaggcgg acaggccgct tgagggaatc gagaagcttt gcctgctccc ggatctggtc
     8221 gggagtgagt tccgtcagag tggcaatttc gtaacggcgg cagcagtata tctggtaggg
     8281 ctccttcccg atgtccagga gggccagacg ggcgatgagg ggtttcaaac catcgccgtt
     8341 gccgccctgt tggagggatt gcgcaccttc tttcttcacg ttttctcggg cggatccccc
     8401 tcgaacgtct cccggcccct gctgtttccc ctgcacctcc gcatcggttt ttgcgggggc
     8461 attctttgcc ggaacctctt gggactgatg cttctcttgt tcgcctgctt cctcctgcgg
     8521 ttgaaacacc ggggattcac ccccgctttt ttccagccag tcaaggagga gcttccccgt
     8581 ttccacagta gggacgaaat accgaccgtc gaaaagggtc gtccggtctt tcgtggccgc
     8641 agcggtgtgg ttatgatcga gatccatgaa gacggtaaac tcgtactcca tgccgtcccg
     8701 ctggatggga cccatgccga gtttcttgac gtgtttcttc ccgttttcct ccacctgggc
     8761 gtattccatc ttgctccgca tggtggcgat gatgtggcat ttggactgga gcatggcatc
     8821 caccagctcg ttgtgcttcg gcgtcacctg ccgccaggcc gcccaggagt tgcccgtctt
     8881 gtcggcgatg tggccgtgga cgtcgagcaa tcccccttgt cccacccagg cgtgggagag
     8941 ggaatcaata aggatgatcc cgtagccgag ttcctccgcc tcttttattg cctcgatgta
     9001 ctttctcggc tcgaaggggg cctcgatgcc acagacatcg tatgcgccga gatgatcgta
     9061 gagctccccg gaaccccgtt ccgtgtcgat cagggcaatc cggccgccga gaccgaaggc
     9121 gatgaggagg gccgagtaag tctttcccgc accggcggga cccgcgatgg ccagccggag
     9181 cttgcctttt tttctttccg ctttatgaaa catggtcatt ctctcctttc atggtggaga
     9241 tcgttcccgt gatcgaggga ttcatcctca attggcgagg ggctgtcggg gacgatgggg
     9301 gttggctttc ggtgaaacgc gatgacgacg gtccttttgc ccttgtggat ccggccgacg
     9361 cagagcaccc ggagagtgtt ctccggctgc acatggcgct gattgattct ctcagcatat
     9421 ttgctgtcgc cggcctcagc actgggtttt gcttccgtca cctgccataa atcatatttg
     9481 ttcatctacg cacctccttt cacccccgga cagctcccga tcagatttct cccgctccag
     9541 tctgctcaga aaccgggtaa cggcggtttg ccgccgaatc tttcccgacc ccggcggtcc
     9601 gccgagcttc cgcacccgtt tcatggcgct cgtgtaaccc tcttccaggc accggacgac
     9661 atcctcggca aagaggtcgc tggtcccgta gaggaggaat agacactcca gggacagggc
     9721 caggtcgtaa aaggcctccc ggatcacctg ggtcgtatct ttgggcggtt gctgtttcat
     9781 gtctcctgtc tccctgtgcg aggtcctcct actcttatta tgtcagtgcc ccccttccag
     9841 gggacaaaga aagttgaaaa atattttttc ttcattcatt ttgggatgtt acgtagatat
     9901 ttccggatag cggccaggca ccgctgccgt cgcttcttca ggcgctggta ttccttcggg
     9961 tcggtggcca ggtccttgaa gggacgttcc ttgatcacgg tctcgtagat gatctcccga
    10021 tcctcggcac cgatgacccc catcgcaacc agaccggtga gaaacgactc ggcatcggtg
    10081 aggtcgtagc gccgccactc cccggtggcg ttttcttcgc cctggtctcc ttgttctccc
    10141 tgctctcctt gaggaagaac ggtcatcctg ccgggataag acgacggcat ctcatcgcca
    10201 taattaccat cccaaacatc gtcatgattc ccatccgggg cggcggcaat atcccacgtc
    10261 ttttcaatct caacatcccc cttatggttg ccgtgaccta tcggcatcgg attatcgccg
    10321 tccaaggtgg cgctcccaaa agttgtcaaa cgccccccgg caatctcccg gttcagcagg
    10381 ctgcggaccc ggttgcgcaa ggggccgaca atctgcatgg ccaccttttg gggccgcacg
    10441 ggcgccgtcg cgatgatctc caggaggagg gtgcagacat cctggatgaa atcttcttca
    10501 tcgagacgga agttactttt gcgggcgagg cgatagaggc tggcgatggc gggacgaaag
    10561 aggatgatga ataacccgga gaggaggcgg ccgggaccgt cacgccgata aaccgtgatg
    10621 agaaaggaca ggagggcgtc tttctcggcg tactccttct ccacgtcgtt aaagaaggcg
    10681 attaatgcgg tggcggaggg atatgccacc agatggggac attcctcctg gaggcggcga
    10741 tagaggctcg tgaaccgctc cgaggcaagt tcttcctcga tacggagaaa agaaaaaccc
    10801 atgcgcggga tgcagcatgg gcttcgggag cctcggagct tgctccttca aatgaatcag
    10861 atgttcagaa ggttatgatc tttttacagg tccggcagcg gacggcgacc aggtcaccta
    10921 cggggcgcac ggtcttccga ccgcaggcac agacggcaac agccaggatg atcccggagt
    10981 gaccgtcact gaagacgcgc ccattggctt tttcaccgca ggggcagcgg atcttcaccc
    11041 ccagacggga caggggcggc ctggccatct ccaagagctt atggccgtcg tggggacatc
    11101 ggcattcata cataagatcg atcgctccgc agaattactg gactttttta gagtatggag
    11161 cgacttatag cggatcggga agaaaagtgt ctcgtccggc taatcgattc tttatcgatt
    11221 tttctcgcta attctcgccg gaaatcgtca attatcgtca attctcacct gtaggaaacc
    11281 acggcgtcgt caccatcggc tggctgtcag ggaagaaggt tcgcctcttt cgtgtgcttg
    11341 tcagggatta tcgcgtatct attccaataa ctgatgtctg tcgggaaaat gtaccccttc
    11401 tccggtagcg tcaggggagg atgcaaatct cctccggcgg caggttgagg atgtaaccgg
    11461 agccgaaacg gctgacgatt agctgtcgca tctccccccg agcaggactg ccggcatcgg
    11521 cggcgctcgc ggcagccttg gcaattccgg cgctgatccg gtatttgtga tccgtaatct
    11581 ggcgatcgta aggccgggta ttatcatcgc catcgccgtc accctccgcc cagatgcgat
    11641 cgtagatctc cgcacgggtg accaatttac cgggactgcc ggccaggagg gccagcatat
    11701 cgaaagcctt gccggtcaag agaaaagcga ttccccggta gaaacagcgg cgacggcggg
    11761 cgtcgatgac cagctcgggg ggtgaggaga tcaattcgcg caggttctcc gcctgggcga
    11821 gccgtctccg gaagtcggca agcagcttcg gatcggcgat gatctccacc agggagggag
    11881 ggaatgcggg agtctgttcc tcccggaccg taagctcctc ggggaggcga taacccttgg
    11941 gactggcctc tttcaggagg aaatgcgcct cccggtagct atccgccgca accgccggtt
    12001 gcagctccaa acgctggtcg agccattccg ccactgtacg ggggacgagc ttccgaaggc
    12061 tctcggcgca atggatagag acggactgtt tgatctcccc gttcacctcc gactggcatt
    12121 cgagatagtc cttgttgatc cgccagaggc ccacttggga aaggatcatc ccgatgtccg
    12181 tgaacccgga gatcaaacgc tgggagaggt tgtcgatctt ccagacctcc tccttcccct
    12241 gaccggcctc attggccgct ttatagagga gttcggacag gaggcttata tcctccccaa
    12301 tcttcttcat ggcgccgctg aaggcgggca gttccgccag gatatccgcc tggatctctt
    12361 tgacccgttc catatgcctc tcccgcagaa tccggatgat gtgcggatcc agagtcaggt
    12421 catccagaag atcgatcacc atgacagcct cttcccctcc tttgataatc gtattttttt
    12481 agatctggtt gtgccgggtc ccatcctttc ttgcctcact ggttcatcaa acagcgtatc
    12541 aagcgcgccg gtgataacgg aacaatacga gcatatcata ttccggggca gttgttacca
    12601 ctatttacct gattttaaaa ggaaataata tttatttcag aatctttgtc ccctaaacgg
    12661 ggctccctga cataataagg gtagaggggg atttccccag ggaggcggca gaaggaataa
    12721 acctgtttag tcacaacaat cagggagaca aatccatgaa ttttgagctg gaagcgaagg
    12781 acatagaggc catcgccggt cgcgtaatag aaaagcttgt gccattgata cgggaacttc
    12841 agaaaccgga tcgtgttatg gacattctgg aagtctcggc attgcttggc aaaagcaagg
    12901 gccaaattta ccagtgggtc aacctagcca gccacggcct gtccgatttc ccttttcaga
    12961 aggccgggcg gtcattgcga ttctcggaag atgcgattca ggagtggatg aagaaaaaat
    13021 ctttgcgccg ttagaaatcc gttagaaaga taatctaact tagcttaata tttgaatatt
    13081 taatatatgc attaggttat ataaccaaag gtcgcccctg aaatttcatg actttccggg
    13141 caaatatcta tatacttacg tcatggatga gcctgcatca atcggtgttc ggcgtaggtc
    13201 aaatacaaaa acggaaaggg ggtgataaaa accggacgga gagtccgcaa aggatcggct
    13261 gacgttcctg gagatcgagg ccagcgccgc ggtgcaggaa acgacagaca tcggaggtcc
    13321 ataatccatg acaggcagca tccagaagaa gggcaagact tattacgccg tgctccctat
    13381 caacggaaaa cggaagtggt tcaaaggggg aacgaagcga gatgcggaac gggtccttgc
    13441 cgagaagttg gcggaagtaa gccagggttc ctacagagaa atacaaaaaa taagtttcaa
    13501 ggaattcgcg acaatgtggt tgaaaactca tgctgaaaac aatgtgaagc catcgaccct
    13561 ggccagttat aaacatgtaa tcaataaaaa ccttgtgccc cggtttgggt ttctccagat
    13621 gtccgacatc acgaccggcg ggctgcaagt ttatgtagca gagcggagaa aggccgtcgc
    13681 gagtaaaagt gttttgaatg aagtcgtgct cctcaaacag ttgttcaagc atgcccacaa
    13741 gtggggctat gcgaggagca atcccggcga atatctggaa agaccgaagg tgatcaagtc
    13801 ggagattgaa atcctcgatc cggcggaagt gaaactgtta ctgaacagta ccggcgaact
    13861 ttaccggacc tcctttctga cagccatcct gacgggtgta agggcaggcg aactctgggc
    13921 gttgcaatgg ggtgatattg attggcactc aaaacgcctc tgcatccggc gctcggtctg
    13981 gaaaggtgat ttccagtcac cgaaatcaca gcagtccgtt cgtaagattg atctgccgga
    14041 cagcttgatt ccggaactga agaaatggaa ggtacggtgt cctatcagcg aaaaagatat
    14101 gatgtttccc accgcggaag gaaagatcac cgatcacgac aatgccagaa acaggtactt
    14161 caattcgtcg ctgcggaaag cgggactcag gcatgtatca tttcacagcc tgcggcatac
    14221 aaacgcttcc atgcggatcc tggccgggca gaatatcaag tacatctcca cacagctcgg
    14281 tcactcctcc gtgaagatca ctttggacac ctacggccac ctgtttaatg accaggattt
    14341 caatcggcac caggttggtc tcctggaaac tgttttcagc gcgtctgtta gaaatccgtt
    14401 agaaaaagtc tcgccaaaaa cggaatttga aacagatttt gttgcgcccg ttagaaatcc
    14461 gttagaaaaa cccctccaaa atgcagaaaa gggattagcc gcttcagcta accccctgta
    14521 atcatttggt agcggggaga ggattcgaac ctctgacctt tgggttatga gcccaacg
//
LOCUS       DAOSMF010000035        14576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_4854_length_14576_cov_24.625301, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000035 DAOSMF010000000
VERSION     DAOSMF010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 14576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14576
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4854_length_14576_cov_24.625301"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..943)
                     /gene="ybgF"
                     /locus_tag="PLT63_05435"
     CDS             complement(113..943)
                     /gene="ybgF"
                     /locus_tag="PLT63_05435"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HOD98202.1"
                     /translation="MKIFSRFLLVLMLLVPFSGCATTGDLQRVQGNLGGKIGDHDASI
                     SGLNEQAEANKKAIDELRTKQADVNADLTVIREQLQQLRGDMDQLQKEAADIRSWAAD
                     TKEWKEKLDQTSFKVNFIENFLGIGKKDERPAGNAEKHHKNQVKNGVKAKTDKESLYA
                     AAYELYKEGKYEKARGEFQHFLKAYPKTEYSGNAQFWIGECYYFEKSYEKAILEYEKV
                     IKNYPDGNKVPQALLKQGLSFLELGDKASARLILQNVIKDYPNTSQARVARSKLLEIK
                     "
     gene            complement(1065..2369)
                     /gene="tolB"
                     /locus_tag="PLT63_05440"
     CDS             complement(1065..2369)
                     /gene="tolB"
                     /locus_tag="PLT63_05440"
                     /inference="COORDINATES: protein motif:HMM:TIGR02800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HOD98203.1"
                     /translation="MKKRCEWQKILVGLLALFSLSVPVTGSAKVYIDIDAPGFQKFPI
                     AVDVFTKGGGGTDQERNAVWFPDQISKYLDMTGYFRIIPRNAFLEDPQKDRTKIIFSD
                     WQTIGADYLVKGHFQSQNRRLTVEMRLYDTVRGEVIREKKYTGRSDERKEMVIKFVNE
                     ILLALTGERGVFNTKIAYVLKRGINSEIQTIGFDGTGQERVTNYGSLTLAPRWSPNGR
                     HISFTSYKDNNPDFFVRDMKTATRKKVAGYPGLNLSGAWSPDGRKILLTLSKDGNQEV
                     YILDVGSGYLQRLTYEYAIDVSPVWSADGKKIAFVSNRAGSPQIYVMDSDGRNVRRLT
                     YEGNYNTSPSWSPKGNRIAYESTVGGNFQIFTISAEGGPPQQLTTQGGEHKHPSWSPD
                     GRYLAMTVKSGGVNKVAVMNANGTNLRILCEGSNPAWSPFLE"
     gene            complement(2398..3120)
                     /locus_tag="PLT63_05445"
     CDS             complement(2398..3120)
                     /locus_tag="PLT63_05445"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOD98204.1"
                     /translation="MTSLDYPGMRDRRGDRLSRLVILSLLVHLLLLSLAIFLPIRSAT
                     PRFTLGPVYSVNLVSVSDSILRRQPAADPLQDIAGAVLSTGTRAFKKSVRMEAPPIRR
                     VDTKQNLRNPAVEKLKKKLGDKTPASAESASPATSAAAGQIDDRLADYYRVIWSRIKS
                     QWALPGGINPRNDVEAVVNIRILKDGSIAEMAFERKSGLAYFDNSVLRALKKAHPLPP
                     LPVWYRDNGLEIGIRFLSSDLR"
     gene            complement(3117..3542)
                     /gene="tolR"
                     /locus_tag="PLT63_05450"
     CDS             complement(3117..3542)
                     /gene="tolR"
                     /locus_tag="PLT63_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HOD98205.1"
                     /translation="MRFSRKRRFFDSKLVAEINVTPLVDVILVLLIIFMVTAPMLSMG
                     IDVNLPRVKSKTIDVNEEKLVLTINKDREIFINRTKILKEDLKVKLENIFAARLDKEI
                     FLRADKTVAYGFVVEVMSEIRKAGVDKLGMVTETPEETK"
     gene            complement(3555..4304)
                     /gene="tolQ"
                     /locus_tag="PLT63_05455"
     CDS             complement(3555..4304)
                     /gene="tolQ"
                     /locus_tag="PLT63_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HOD98206.1"
                     /translation="MKEIPYVAMAGLGGQFQGSVFGMILDAGLMVKFVLLLLLGFSVV
                     SWAIIWLKYRTLSKVKKENEQFLDAYVKSTRLADIFSEAKKFPESTLAEVFRSGYGEL
                     VKITKAIRERVAGKESVPQSEPVFELKGLDNIERSLSRATGSETDKLEKALGFLATTG
                     SACPFIGLFGTVWGIMDTFKDIGARGSATLAVVAPGISEALVATAAGLAAAIPAVIFY
                     NYYTNRVKSLAMEMEAFATELVNIMERNYFK"
     gene            complement(4393..5316)
                     /gene="epmA"
                     /locus_tag="PLT63_05460"
     CDS             complement(4393..5316)
                     /gene="epmA"
                     /locus_tag="PLT63_05460"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HOD98207.1"
                     /translation="MHDPWPLAKKRRTLALRARIVGELRRFFAARDYLEIETPCLIPA
                     PAPEFHIDTLACEGWFLQPSPELCMKRLLAAGYDRIFQICRCFRKGERGARHLPEFTM
                     LEWYRREADYRDLMTECEDLLIEAAAALGYGDVVTYQGEPLRLGKPWERLTVAEAFAR
                     FAPLPVAEALAGDCFDELLAIHVEPRLGRGRPTFLCDYPLPLASLARGKRGEAAVAER
                     CELYIAGIELANAFTELAEEREQRRRFAREEEARRRAGKPHYPSPDKFLESLTDLGDA
                     AGIALGVDRLVMILADAPDIDAVTAFTPETL"
     gene            complement(5384..5947)
                     /gene="efp"
                     /locus_tag="PLT63_05465"
     CDS             complement(5384..5947)
                     /gene="efp"
                     /locus_tag="PLT63_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOD98208.1"
                     /translation="MYTASDLRKNLRIKIDDEPYVVTEFNFTKPGKGQALYRCKLKNM
                     ISGSQFERTFRSVDVFEPADLQEKKMQYLYDQEGKYCFMDNETYEQVLLSEDQVGDAR
                     NYLIENIEVEILLFEDRPLGVSLPNFVDFIVTKAEPWARGDSVSGNTKPVTVQTGYVL
                     QVPPFIEEGEKIRVDTRTGEYLTRVKD"
     gene            6067..6483
                     /locus_tag="PLT63_05470"
     CDS             6067..6483
                     /locus_tag="PLT63_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98209.1"
                     /translation="MSKKFDSWTIYAPESSQKKSFDLRNESAYLGTIMKRRHLYILYL
                     GLWILAAFLLLSGFPKELAHHWAGRGSLALPLTIIYGLPIFLLYLLTAFLFDFRGDPA
                     GKDQFLLFLRRRRMVVIGFLFALAALILLILKTFTA"
     gene            complement(6502..7479)
                     /locus_tag="PLT63_05475"
     CDS             complement(6502..7479)
                     /locus_tag="PLT63_05475"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOD98210.1"
                     /translation="MKKNIYIRDIKPGEKIYDFFFVTEKNLAYSQKGAPYLNLRLKDK
                     TGELDGKVWDNVQELDRAFRKGDFVYLQTRAVQYKNAIQLAVMSARRAAPEEIDAAEF
                     FPVAKEDPERMFRELESFADRVQTPALRDLLQTFLADEEIAALLRRAPAAKGFHHVFI
                     GGLLEHTLSVVRILDLLSGHYEGLNHDLVITGGILHDIGKIYEYSYDGLIDYTDEGRL
                     IGHIVMGVEMVDRKIAALPDFPAQLAMELRHVLLSHHGVLEFGSPKRPKTLEALLVHY
                     VDDLDAKINAMQTFVAGDGNEDSDWTPFHRLLERYLYKGKNAGRGEGAT"
     gene            complement(7476..8228)
                     /locus_tag="PLT63_05480"
     CDS             complement(7476..8228)
                     /locus_tag="PLT63_05480"
                     /inference="COORDINATES: protein motif:HMM:NF014671.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HOD98211.1"
                     /translation="MSGGDRRPAGTERCSVRSFTGGSWREKEAEVVREISLDVCLNGR
                     KLAAIACAGLHVEELAVGWLRSEGIIRNAREIAAATAGREGWSVDVRTAADWSGAGPS
                     GEPEPLPLPAAGAATVSPERIFTLMGQMVEAADIHRRSRGTHGAALAGTAGIITVRED
                     IGRHNCLDMLSGYALLNGIAGADKMILRTGRVSSDIVIKIWRMGVPAVISLSVPTALA
                     VRIAERAGITLVGAVRAPSLTVYTHPERVGGA"
     gene            complement(8225..10138)
                     /gene="selB"
                     /locus_tag="PLT63_05485"
     CDS             complement(8225..10138)
                     /gene="selB"
                     /locus_tag="PLT63_05485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HOD98212.1"
                     /translation="MKHIILGTAGHVDHGKTSLVKALTGIDTDRLKEEKERGITIELG
                     FAALRLPGGTTLGIVDVPGHERFIKNMVAGASGVDLLLLVIAADEGVMPQTREHLHIC
                     TLLGIRRGLVALTKIDMVDEDWLALVRDDVQDYLQGTFLAGAPVVPVSAATGAGLPEL
                     VAALDALAATVEEKRDHGVFRLPVDRVFSMRGFGTVVTGTLISGTVGVGDEIELLPSG
                     RQAKIRTIQVHNQGVERAEAGQRTAINLQGMDRAAVERGEVMIRPGTVAVSRRLDVLL
                     NYLESNEKALKNRSLVRFHVGTRENIGRIILLDRDEALPGAGVNGQMIFAEPVVVMAG
                     DHFVIRSYSPITTSGGGIVLDPLPRKHKRMQAAVGEEFRLLAAGTEEQRVAVIVARAG
                     SEGANITRLVMRTGLAEGRLRKIIDAMLNARQVVLLDSETLLVVSAAAYASLQEQLLR
                     KLGGYHEKNPLREGMPKEELRMNVGAFIQAKLFNKALKDLEKVGKVVLEREAVRLPEH
                     QVRLEEDLETLKGRILELYLQAGLTPPTIREVYGRFPDRAKGVDSVRTILLREGKLVK
                     VNEDLLYHRAVLDKLQEDYREMLLRDGRATPVTFKELTNLSRKFIIPLMEYFDTAKMT
                     VRSGDYRLLREKR"
     gene            complement(10507..10956)
                     /locus_tag="PLT63_05490"
     CDS             complement(10507..10956)
                     /locus_tag="PLT63_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98213.1"
                     /translation="MPRLLPPCRRRPAVPANEAPDEDLLPFPAQISVLHFVAELAEEM
                     YSKAAAAAGNHFRQTGLLALAEIIDVEGDALETDQQFQDVINHRQGHGDLFTLTGPIF
                     MLNDVGEDLFDNEFGLSGDVAVGAGSADIYGRGGLQYGYRLSFIGYS"
     gene            11118..11627
                     /locus_tag="PLT63_05495"
     CDS             11118..11627
                     /locus_tag="PLT63_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98214.1"
                     /translation="MYMFLLFDPTPRKPYYPPPHHREIIEKICRHVNMAVSFRASPGE
                     IALPVRGEMETKTDVYQAGHVVVHRWGGDTLSQVRTILRHWRLDRLETIYLYLPLSEP
                     STAVLCRSSEEMGFFFSGLMPGRAGEDWLVLQYLNNQRYDYGLIKSAFPFGQELIDYV
                     RTCDPEGDL"
     gene            complement(12013..13320)
                     /locus_tag="PLT63_05500"
     CDS             complement(12013..13320)
                     /locus_tag="PLT63_05500"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOD98215.1"
                     /translation="MDDTVFSLHLWPQAILHIDGDAFFTSCEEIIHPELRGKPIITGA
                     ERGIVACASYAAKKMGIKRGVSLHDAKTICPGLVVLPSDYETYSLFSRRMFSIMRRFT
                     PSVEEYSIDEAFADITGLRRPLKSSYENIARKIKKDIQREIGFTVSVGLSLSKVLGKI
                     GSKYQKPDGLTVIPGRSIAYFLKDLPVDKIWGVGPATTAHLNKLGVSTALEFARMPEQ
                     KVRKSFTKPTIEIWQELRGQSVCPVVSEEKSTYASISKTRTFAPSTSDADYLFAQLLR
                     NLESACIKARRYKLAPRRIALFLKTDNFLYSGCELRLNTPSAYPMDLMDLARNAFDSL
                     YRKNISYRATGVVLMEMCSDLPVQYSLFDDAVRVDKVRTLYDAVDEICRRFGKHALHL
                     GGSHVIEVSGKGKRGKSTSREAMQFFGETKRQHLGLPILHVKV"
     gene            13687..14031
                     /locus_tag="PLT63_05505"
     CDS             13687..14031
                     /locus_tag="PLT63_05505"
                     /inference="COORDINATES: protein motif:HMM:NF017757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOD98216.1"
                     /translation="MKTTRPISWIKAARKNFEDFPDVVQHDMLTALTIAAEGEKSDSA
                     KPFRGIEGGVFEIAVRYRGDTFRAIYAVKMDSDIWVIHAFQKKSKKGIKTPLMDINII
                     HERLRQLKEALK"
     gene            14028..14351
                     /locus_tag="PLT63_05510"
     CDS             14028..14351
                     /locus_tag="PLT63_05510"
                     /inference="COORDINATES: protein motif:HMM:NF025120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOD98217.1"
                     /translation="MTEKIELIKGSGNVYADLGRPNAGLEQARALIASEIIRALDARG
                     LSTREAERVTGVGHAEFSRIRNAKIARFSLDRMIAILGKLDEDVEVIVSFQPRRPIVQ
                     DVTHI"
BASE COUNT         3070 a         4210 c         4017 g         3279 t
ORIGIN      
        1 gagtacgaaa gcgtaacact ggaaacgatg accgccgccg gggcggagtt gtttggcctg
       61 cccgttcccc cgggcgcgaa ggtgagaaga ctggaataat tgttccgccg tcctatttga
      121 tttccaggag tttggaacga gccacccggg cctggcttgt gttcggatag tccttgatga
      181 cgttctgaag gatcagcctg gcactcgcct tgtcgcccag ctccagaaag gaaagcccct
      241 gtttcaacag ggcctggggg actttgttgc cgtccggata gttcttgatg accttttcat
      301 attccaggat ggccttttcg tagcttttct cgaaatagta gcattccccg atccagaact
      361 gggcgttccc cgaatattcc gttttgggat aggccttcag aaaatgctgg aattctcctc
      421 gggccttttc gtatttaccc tccttgtaca gctcgtaagc ggcggcatag agagactcct
      481 tgtccgtctt ggccttgacg ccgtttttaa cttgattttt gtggtgtttc tccgcattac
      541 cggccggccg ttcatccttc ttgccgatcc cgagaaagtt ttcgataaaa tttaccttaa
      601 atgaggtctg gtcaagcttt tccttccatt ccttggtatc cgcggcccag ctccggatgt
      661 ccgccgcctc tttctggagt tgatccatgt caccccggag ttgttgaagc tgctcacgaa
      721 taaccgtgag gtccgcattc acatcggcct gtttcgtacg cagttcgtcg atggccttct
      781 tgttggcctc cgcctgttca ttcagtcctg atatcgacgc gtcgtgatcg ccgatcttgc
      841 cgcccaggtt cccctgtacg cgctggagat ccccggtagt ggcgcagccg ctgaagggca
      901 ccaataacat caggaccaag aggaaccgcg agaaaatttt caaggtacat acccccttaa
      961 ttttagtgcc tttatgacct caggagcggt cggtttttgt gccttttaat caaaaagagc
     1021 ggcaaaggtc aactattaat ttttccttgt tcatcgattg acggttattc caaaaaagga
     1081 gaccaggcgg gattggagcc ctcacagagg atgcgcaggt tggtcccgtt ggcattcatg
     1141 acggcgacct tgtttacccc cccgctcttt accgtcatgg ccaggtatct gccgtcggga
     1201 gaccaggagg gatgcttatg ttcgccgccc tgggtggtca actgctgggg cgggcctcct
     1261 tcggcgctga tggtgaagat ctggaaattg ccccccaccg tgctttcata ggcaatccgg
     1321 tttcctttcg gagaccagga gggagaggtg ttgtaatttc cttcataggt cagcctcctc
     1381 acgtttctcc cgtcactgtc catgacatag atctggggtg agccggcgcg attggaaacg
     1441 aaggcgatct tcttgccgtc cgcggaccag accggcgaga catcgatggc gtattcataa
     1501 gtaagccgct ggagataacc gctccccaca tccaggatat acacctcctg attgccgtcc
     1561 ttgctcaggg tgagcaggat cttgcgcccg tcgggcgacc aggccccgga aaggttcaag
     1621 cccggataac cggcgacctt ctttctggtg gccgttttca tgtcccggac aaagaagtca
     1681 ggattgttgt ccttgtagga ggtgaaggag atatggcgac cgttgggaga ccagcgcggc
     1741 gccagggtaa gcgacccgta attggtgacc cgctcctgac cggtgccgtc gaagccgatg
     1801 gtctggatct ccgaattaat ccccctcttg aggacatagg cgatcttggt attgaaaacc
     1861 ccccgttccc ccgtcagggc aagcaggatc tcattgacaa acttgatcac catctctttc
     1921 cgttcatcgc ttcgccccgt gtattttttt tctcggatca cttctccccg caccgtgtcg
     1981 tagaggcgca tttccaccgt aagccgccgg ttctgggact gaaaatgccc cttgaccaga
     2041 taatcggccc cgatggtttg ccagtcgctg aagatgatct tcgtccggtc tttctggggg
     2101 tcttcaagaa aggcgttccg ggggatgatg cggaaatagc ccgtcatgtc gaggtattta
     2161 gagatctggt cgggaaacca gacggcattt ctctcctggt ccgttccccc gccccctttg
     2221 gtaaacacat ccacggcgat ggggaatttc tggaagcccg gggcgtcgat atcgatatag
     2281 accttggccg atcccgtgac ggggacagac aaagaaaaca gggccagcaa ccccacgagg
     2341 attttttgcc attcacatct ttttttcatt cctgccgaat ctccctttat cccccgatta
     2401 ccgcaggtcc gacgagagga agcggatgcc gatctccaag ccgttgtccc ggtaccatac
     2461 cggcagcggg ggcaggggat gggccttctt cagcgcccgc aggactgaat tgtcgaaata
     2521 ggccagcccc gattttcttt cgaaggccat ctcggcaatg gagccatctt tcaggatgcg
     2581 gatattgacc acggcctcca cgtcgttccg ggggttgatg ccaccgggca aagcccactg
     2641 gcttttgatg cgggaccaga taacccggta ataatcggcc agccggtcgt caatctggcc
     2701 ggccgccgcg gaagtggccg gagacgccga ttcggcactc gccggggtct tgtcgcccag
     2761 ttttttcttc agtttctcca ccgccggatt gcgcagattc tgctttgtat ccacccttct
     2821 gatcggtggg gcttccatcc gaaccgattt tttaaacgcc cgggtccccg tggacaggac
     2881 ggcgccggcg atgtcctgaa gggggtccgc cgccggctgg cgccgcagga tactgtcgga
     2941 gacacttacc aggtttacag aataaacggg accgagggtg aagcggggcg tcgcggaacg
     3001 gatggggaga aatatcgcca gggacaacag cagcaggtgg accagcagcg agaggatgac
     3061 aagccgggac aggcgatcgc cccggcgatc ccgcatcccc gggtaatcca gggacgtcat
     3121 ttcgtctcct cgggggtttc ggtcaccatg cccagtttat cgacgccggc cttgcgaatc
     3181 tccgacatta cctccaccac gaagccgtag gccacggttt tatccgcccg caggaagatc
     3241 tccttgtcga ggcgggcggc aaagatgttc tccagcttca ccttgagatc ctccttgagg
     3301 atcttggtgc ggttgatgaa gatctcccgg tctttgttga tcgtcaggac gagtttctcc
     3361 tcgttgacgt cgatggtctt ggatttgacc cggggcagat tgacgtcgat ccccatggac
     3421 agcatgggcg ccgtgaccat gaagatgatc aggaggacca ggatcacatc gaccagcggg
     3481 gtgacattga tctcggcgac gagcttgctg tcgaagaaac ggcgttttct cgaaaatctc
     3541 atcgtccccg cccgctattt gaaatagttc cgttccatga tatttaccag ttccgtggca
     3601 aaggcttcca tctccatggc caggctcttg acccggttcg tatagtagtt atagaagata
     3661 acggcgggga ttgccgccgc gaggcccgcc gccgtggcca ccagggcttc ggagataccc
     3721 ggggcgacta ccgctagggt cgcggatccc cgggcaccga tatccttgaa ggtgtccatg
     3781 atcccccaga cggtgccgaa aagaccgata aacgggcagg cgctgcccgt agtcgccagg
     3841 aatcccaggg ccttctccag tttatccgtt tcgctgcccg tggccctact cagcgaccgt
     3901 tcgatattgt ccagaccctt gagttcgaaa accggctcgc tctgggggac cgattctttt
     3961 cccgccaccc gttcccgaat cgccttggta atttttacca gttccccgta tccggaacgg
     4021 aagacctcgg ccagggtcga ttcgggaaat tttttcgctt ccgaaaagat atcggccagg
     4081 cgggtggact tgacataggc atcgagaaac tgttcatttt ccttttttac cttgctcagg
     4141 gtccggtatt tcagccagat gatggcccag gaaacgacgg aaaaacccag gaggagcagg
     4201 aggacgaatt tcaccatcag gcccgcgtcc aggatcatgc cgaagacgct tccttgaaat
     4261 tgtccgccca gcccggccat agcaacataa ggaatttctt tcacttttgt cctctccaaa
     4321 agttgtttta cacttggggc ttacctgtaa aggaaaagcc gccccttgtc aacaagactt
     4381 ttcataatct attcataatg tttccggggt gaaggcggtc accgcgtcta tatccggggc
     4441 atcggcaagg atcatcacca ggcggtccac ccccagggca atccccgcgg catctccaag
     4501 atcagtcaac gattccagaa acttatccgg agaaggatag tggggtttgc cggcccgccg
     4561 acgcgcctcc tcctcccggg caaagcggcg gcgctgctcc ctctcctccg ccagctccgt
     4621 aaaggcgttg gccagttcga tgccggcgat gtacaattcg cagcgctccg cgacggctgc
     4681 ttccccccgt ttcccccggg ccagggaagc caaaggcaat ggatagtcac acagaaaggt
     4741 cggcctcccc cgtcccagcc gtggttccac atgaatcgcc aggagctcgt cgaagcagtc
     4801 cccggccagg gcctccgcca ccggcagggg ggcgaaacgg gcaaatgcct ccgcgacggt
     4861 caggcgttcc cagggtttcc cgagccgtag cggctcgcct tgataggtca ccacgtcgcc
     4921 gtaaccgagg gcggcggccg cctcgataag gagatcctcg cattcggtca taaggtcccg
     4981 gtaatccgcc tcccggcggt accactccag catggtgaat tcgggaagat ggcgggcgcc
     5041 ccgctctccc ttgcggaagc accggcaaat ctgaaagatc cggtcgtaac ccgccgccag
     5101 gaggcgcttc atgcataatt ccggcgaagg ctggaggaac cagccttcgc aggccagggt
     5161 atcgatatgg aattccggcg ccggggcggg gatcaggcag ggggtttcaa tctccaggta
     5221 atcgcgagcg gcaaagaagc ggcgcaactc ccccacgatc cgggcccgga gcgccagggt
     5281 acgacgtttc ttggccaggg gccagggatc atgcatgaca acccctcacg aagggggacc
     5341 gaggccatcc cttccttgcc gcggcgatgg tcagggaaat acttcagtcc ttgacccggg
     5401 tgagatattc cccggtgcgg gtatccacgc ggatcttttc cccctcttcg atgaaagggg
     5461 gcacctggag gacataaccg gtttggaccg tcaccggctt ggtgttcccc gaaacactgt
     5521 cgccccgggc ccagggctcc gccttggtga cgatgaaatc cacgaaattg gggaggctga
     5581 cccccagggg acgatcctcg aagaggagga tctccacctc gatgttctcg atgaggtagt
     5641 tccgggcatc acccacctga tcctccgaaa ggaggacctg ttcgtaagtc tcgttgtcca
     5701 tgaagcagta tttaccctcc tgatcgtaga ggtactgcat cttcttctcc tggagatccg
     5761 ccggttcgaa gacgtccacg gaccggaagg tacgttcgaa ctgggagccg ctgatcatgt
     5821 ttttcagttt gcaccggtag agggcctgtc ccttccccgg cttggtgaaa ttgaactccg
     5881 tgacgacgta cggttcatcg tctattttaa tccgcaggtt ttttctcaaa tcgctcgctg
     5941 tgtacatccg ctaccccttt gggaaaaagc ccggcggcga caagtcccct ttcgccccca
     6001 tttccgttcc ctttcccttt ttttgatttc gaactgagga ggacctcata aattattttc
     6061 ccgtttatgt caaagaaatt cgactcctgg acgatctacg ccccggagtc gtcgcaaaag
     6121 aaatcgtttg acctccggaa cgaaagtgca tatttaggga ccatcatgaa gcggcggcat
     6181 ctctatatcc tctatctcgg gctctggatt ctggcggctt tcctcctcct gtccgggttc
     6241 ccgaaagaac tggcccacca ctgggccggc cgggggtccc tggccctgcc gcttacgatc
     6301 atctacggcc tgccgatttt cctcctctac ctgcttaccg cctttctctt cgacttccgc
     6361 ggcgatccgg ccggcaagga tcagttcctc cttttcttgc ggcggcgccg gatggtggtc
     6421 atcgggttcc tgttcgccct cgccgccctt atcctcctga ttctgaagac cttcacggct
     6481 taagcccgcc ccgctgcccc ttcaggtcgc gccttccccc cgacccgcgt tctttccctt
     6541 gtagaggtag cgttcgagga gacggtggaa gggggtccaa tccgaatcct cattgccgtc
     6601 accggcgacg aaggtctgca tggcgttgat cttggcgtcc agatcatcca catagtggac
     6661 gaggagggcc tccagggtct tgggccgctt cggggagccg aactccagga cgccgtgatg
     6721 gctcaaaagg acatgtcgta gctccatggc cagttgagcc ggaaaatcgg gaagcgccgc
     6781 tatcttgcgg tccaccatct ccacccccat gacgatgtgg ccgatgaggc gtccttcatc
     6841 ggtgtagtcg atgaggccgt cgtaggaata ctcgtagatc ttgccgatat cgtgcaggat
     6901 gccgcccgtg atgacaagat cgtggttgag cccttcgtag tggccgctca gcaggtccag
     6961 gatccgcacc accgacagcg tgtgttcgag cagaccgccg ataaagacat ggtgaaatcc
     7021 cttggcggcg ggggcgcgcc ggaggagggc ggcgatctcc tcatcggcca ggaaggtctg
     7081 gagaagatcc cggagcgccg gcgtctgcac ccggtcggca aaggactcca gttcccggaa
     7141 catccgttcc ggatcttcct tggccacggg aaagaattcc gctgcatcga tctcctcggg
     7201 ggcggcccgg cgggcgctca tcacggccag ttggatggcg tttttatact gcaccgcccg
     7261 ggtctgaaga tagacgaaat cacccttccg gaaggcccgg tccagctcct ggacgttgtc
     7321 ccacaccttg ccgtccaact cccccgtctt atccttgagg cgcaggttca gatagggagc
     7381 gcctttctgg gaataggcca ggttcttttc cgtcacgaag aagaagtcgt aaatcttttc
     7441 ccccggtttg atgtctctga tatagatatt ttttttcatg ctcctcccac ccgttccgga
     7501 tgcgtgtaaa ccgtcaggga cggcgcccgg acggcgccca ccagggtgat gcccgcccgc
     7561 tcggcgatcc ggacggccag ggccgtcggc accgaaaggg agatcaccgc cgggacgccc
     7621 atccgccaga tcttgatgac gatatccgag gaaacccgcc cggtcctcag gatcatttta
     7681 tccgcgccgg cgataccgtt cagaagggcg taaccgctca gcatgtcgag acagttgtgc
     7741 ctcccgatgt cttcccggac cgtgatgatc cccgccgtgc cggccagggc ggcgccgtgg
     7801 gtcccccggg agcggcggtg gatgtccgcg gcctccacca tttgccccat gagggtgaag
     7861 atccgctccg gagaaaccgt cgccgctccg gccgccggca acggcagcgg ctccggctcc
     7921 ccgctcgggc cggcgccgga ccagtcggcg gcggtccgga cgtccaccga ccatccctcc
     7981 cggccggcgg tcgcggcggc gatctcccgg gcgttccgga ttatcccttc gctccggagc
     8041 cagcccacgg ccagttcctc cacgtgcagg cccgcgcagg cgatggccgc cagtttccgg
     8101 ccgttgagac aaacatccag ggaaatctcc cggactacct ccgcttcctt ctcccgccag
     8161 gagcccccgg taaaggagcg gacggaacat ctctcggtcc ccgccgggcg gcggtccccg
     8221 ccgctcatcg cttttccctc aggaggcggt aatccccgga acgcaccgtc atctttgccg
     8281 tatcgaaata ctccatcagg gggatgatga atttccggct caggttcgtc agttccttga
     8341 aggtaacggg cgtcgcccgg ccgtcccgga gcagcatctc ccggtagtct tcctggagtt
     8401 tgtccagaac ggcccggtgg tagaggaggt cttcgtttac cttgacgagt ttcccctccc
     8461 ggagcaggat ggtccggacg ctgtccaccc ccttggcccg gtcggggaac cggccgtaaa
     8521 cctcccggat tgtcggcggg gtgagaccgg cctggagata caactccagg atccgcccct
     8581 tgagggtttc caggtcctcc tccagccgga cctggtgctc cggcagccgc accgcctccc
     8641 gctccaggac caccttgcca accttctcca aatccttcag ggccttgttg aagagtttcg
     8701 cctggatgaa ggcgcctaca ttcatccgca gctcctcttt cggcatcccc tcccggaggg
     8761 ggtttttctc gtgataaccg cccagcttcc ggaggagttg ctcctgcaga gaggcgtagg
     8821 cggcggcgga aacgacgagg agggtttccg aatccagcag gacgacctgc cgggcgttga
     8881 gcatggcgtc gattattttc cgcaggcggc cctccgccag gccggtccgc atgaccaggc
     8941 gcgtgatatt cgccccctcg gaacccgccc gggcgacgat gacggcgacc cgttgctctt
     9001 ccgtccccgc ggccagcagg cggaactcct cccccaccgc cgcctgcatc cgtttgtgct
     9061 tccggggcag gggatcgagg acaatcccgc cgccggaggt ggtgatggga gaatagctcc
     9121 ggatcacgaa gtgatccccc gccatgacca ccaccggctc cgcaaagatc atctggccgt
     9181 tgacccctgc tccggggagc gcctcatccc ggtccaggag gatgatccgg ccgatgttct
     9241 cccgggtgcc cacgtggaac cggacgaggc tccggttttt cagggccttc tcattgctct
     9301 ccaggtagtt caggagcacg tcgaggcgcc gggagaccgc cacggtcccc ggccggatca
     9361 tcacttcgcc gcgctccacg gcggcccggt ccatcccctg gaggttgatc gccgtccgct
     9421 ggcccgcctc cgcccgctcc accccttgat tatggacctg aatggtcctg attttggcct
     9481 gccgacccga gggaagcagt tcgatttcgt cgcccacgcc caccgtcccg gagatgaggg
     9541 tacccgtaac cacagtgccg aagccgcgca tggaaaacac ccggtccacg ggcagacgga
     9601 agacgccgtg atccctcttt tcctccaccg tcgccgccag ggcgtcgagg gcggccacca
     9661 gttccggcaa tcccgccccg gtcgccgccg acacgggaac gaccggcgcc ccggcgagga
     9721 aggtcccctg gagatagtcc tggacgtcgt cccggaccag ggccagccag tcctcgtcca
     9781 ccatgtcgat cttggtgagg gccacgagac cccggcgaat ccccaggagg gtgcagatat
     9841 ggagatgctc ccgggtctgg ggcatgaccc cttcatcggc ggcgatgacg aggaggagga
     9901 gatcgacgcc ggaggccccg gccaccatgt tcttgatgaa ccgctcgtgg cccgggacgt
     9961 ccacgatgcc cagcgtcgtt cctcccggca gtctcagggc ggcaaagccg agttcgatgg
    10021 taatcccccg ctccttttcc tctttgagac ggtccgtgtc gatgcccgtc agggccttga
    10081 ccagcgaggt cttcccgtga tccacatggc cggcggtgcc gaggatgata tgcttcatct
    10141 gttgtccttc ctcatctgcg cccaggaatc ggttcagacc gggggaaaag gtgaaagatt
    10201 tttccctccc cggcggaaga tttctttatc tccgattaca gggcaaataa cagacaaagg
    10261 caattttcac aatggaaatg tccacgggat tgcatctttc accaggaggg gcaagcatac
    10321 gccaacacat tcagttttat tccccgaaca aacgattgat ttttccgcag atcgagggta
    10381 tggaggcgaa tcgcccccgc ttacctttca ggatcgccgc cgaacgtcgc caggcaaccc
    10441 gggaaggggc gacatggatc tcttgccgag ataaaagggg agacccctcc aatggaatcc
    10501 ataactctaa ctatagccaa tgaacgagag gcgatacccg tattgcaggc cgccgcggcc
    10561 gtatatgtcg gcgctgccgg cgccgacagc gacatcgccc gacaaaccga actcgttatc
    10621 gaagagatct tcaccaacat cattcagcat gaatatcggc ccggtcagcg tgaaaagatc
    10681 tccgtggcct tggcgatggt tgatgacgtc ctggaattgc tgatccgttt ccaaggcatc
    10741 cccttcgaca tcgattatct ccgccaatgc gagcaggcca gtttgccgga aatggttgcc
    10801 agcggcggcc gcggccttgg aatacatctc ctcggcgagt tcagcgacga agtgcaatac
    10861 cgaaatctgg gctggaaagg gcaggagatc ctcatccggc gcctcattcg ccgggacggc
    10921 ggggcggcgg cggcacggag ggaggagtcg cggcaagagg aggaagcgcc ggcagcgccg
    10981 cttcggatcg ccgtcaggcg gatgcgtccg gaagaggcgg cgacaatttc caagcttgcc
    11041 tacttcgcct atcgctatac ctacatcaaa gagcaccttt acgatcccga ggaggtcaga
    11101 cgagccggga atcatttttg tacatgttct tactcttcga ccccacgccc cgtaagccat
    11161 attatcctcc cccccatcac cgcgagatca tcgaaaagat ctgtcggcat gtcaacatgg
    11221 cggtctcttt ccgtgcaagt ccgggagaga tcgcccttcc ggtccgggga gagatggaaa
    11281 caaaaaccga tgtctatcag gcaggtcatg ttgtcgtcca ccgctggggc ggcgacaccc
    11341 tgtcgcaggt ccgtacgatc ctgcgccatt ggcgcctgga ccgtctggag acgatctatc
    11401 tctatctgcc cctgtccgaa ccgtccaccg ccgttctctg ccggagcagc gaggaaatgg
    11461 gtttcttttt ttccggactc atgcccggtc gcgccggtga ggactggctg gtccttcaat
    11521 acctgaataa ccagcgctat gactatgggc tgattaaatc agcatttcct ttcgggcagg
    11581 aactcattga ctatgtccgc acctgcgacc cggaaggaga cctatagagc aattccggag
    11641 ccggtgcggg taagagtgaa taatttctcc cgaacatgcc tgtcacggct ttatagttgg
    11701 tctgggtaaa ctggacagtg gcataagttg taaagctaat ctatgcagca caaacaatac
    11761 aaagcgatag ggcgactgaa ccaacaaagg caacgattac ggatcggaag gtgctgtgtc
    11821 atgagcggcg atttttttgg attatgttgc gctgtgtcct tcttgaatcg tgattttggg
    11881 gaaaacgacc tggatcacag agtatatacc gtaatgttca ggcaaaaaga tggataatgt
    11941 aaatgctgcc gtccatcaac attctatgtc cgtgttttgg tatccggtta tacatgtctg
    12001 aaattacgct cctcatacct ttacatgtaa tatgggcagt ccaaggtgtt gccgtttagt
    12061 ttcaccaaag aattgcatgg cctctcttga tgttgacttg ccccttttcc cctttcctga
    12121 aacctcaatt acatgagatc cgcccaaatg aagagcatgt ttcccaaaac gccggcagat
    12181 ttcatcaaca gcatcgtaaa gggtgcgtac cttatcaact cttacggcat catcaaaaag
    12241 tgagtactgg acaggcaaat cagaacacat ctccatcagt acaaccccgg ttgctcgata
    12301 tgatatgttt ttccgataga gactatcaaa tgcattcctt gccagatcca ttaaatccat
    12361 tgggtaggca gagggagtat tcagtctcaa ttcacatccg ctatacaaaa aattgtcggt
    12421 cttcaaaaac aatgcgattc gtcgcggggc cagtttgtag cgtcttgctt tgatacaagc
    12481 cgactcaagg ttgcgtagaa gttgagcgaa caggtaatct gcatcggatg ttgagggagc
    12541 aaatgttctc gtcttactga tggaagcata agtgctcttt tcctccgaga cgacgggaca
    12601 gacagactgc cctcgcaact cctgccatat ctcgatagtt ggctttgtga aacttttcct
    12661 gactttctgc tcaggcatgc gggcaaactc aagcgctgta ctgaccccaa gcttattgag
    12721 atgggcagtt gtagcagggc cgacgcccca aattttatct acagggagat ccttgagaaa
    12781 ataagcgatg gaacggccag gaattacagt taacccatca ggcttctgat actttgaacc
    12841 gatcttgccc aagacctttg acaggcttaa acctacggat acggtgaatc cgatttctct
    12901 ttgaatgtcc ttctttatct ttcttgcaat gttctcatat gatgacttta agggacgtct
    12961 taacccggta atatcagcga aagcttcatc aatggaatat tcttcaacag agggagtgaa
    13021 acggcgcatg atgctaaaca tccggcggga aaacaaactg taggtttcat aatcagaagg
    13081 taatacaacc agccccggac agatggtttt tgcatcatgc aagctgacac cacgctttat
    13141 gcccattttc tttgcagcgt aagaagcgca ggccactatg ccacgttcag cgcctgtaat
    13201 tatcggtttg cccctcaatt caggatggat tatttcttcg caagacgtaa aaaaagcgtc
    13261 gccatcaatg tgcagtatgg cttgtggcca taagtggagg ctaaaaactg tatcgtccat
    13321 gagatgcctt tggaaaatag tcatggacgt tgtagaacga tcgttctgtt gatgcaagga
    13381 aaatattatt agcactgatt accgttcgca atatggcatg ggcagggaaa cctggattaa
    13441 gaatcacagg aagttctgag cgacggcttg agcaaatttc tttacggtca aaattttaca
    13501 gctttccatc gatactcaat gccttgactg gagtttcgag ggtaaacagt agcagatgaa
    13561 aacaatagaa agcagatcaa cattgtagat agatgttgag ttcacgagta agaattgttt
    13621 tgatcgcaag aatagtagtg caaaaaaagc ttgcaccatg gtgtaaaata tgtcaacata
    13681 acgttaatga aaacgacgag acctatatca tggataaaag ctgcacgaaa aaactttgaa
    13741 gattttcctg atgttgtgca gcatgatatg ctgaccgcac ttacaatagc cgccgagggt
    13801 gaaaaatcgg atagtgccaa gccatttcgt ggaatagagg gtggagtgtt tgagatcgca
    13861 gttcgatatc gcggtgacac ctttcgcgcc atttatgcag taaaaatgga ttcagatata
    13921 tgggtaattc atgcgtttca aaagaaatca aagaagggga ttaagacacc gctaatggat
    13981 attaatatta tccatgaacg cttaaggcag ttgaaggagg ccttaaaatg actgagaaaa
    14041 ttgaactaat caagggcagc ggtaatgtgt atgcggacct tgggcgtcca aatgccggtt
    14101 tggagcaagc ccgtgcctta attgcgtcag aaatcatccg cgctttagat gccagaggcc
    14161 tgtcaacacg tgaagcagaa agagtgaccg gagtgggaca tgctgaattt tcgcgcatcc
    14221 gcaatgcaaa gattgcccgt ttcagccttg acaggatgat tgccattctc gggaagcttg
    14281 atgaagacgt agaagtaatc gttagctttc agccgcgccg accaattgtg caagatgtga
    14341 cgcatattta gaagcaaaca tctcttcatg ttcaggaatc gctttccagt tctgcatctc
    14401 attaacttag aggcattaca agttatatta taggagtcac tattttggat gtcagcgagg
    14461 tgctggaaaa attatagtgg accccgaaat ttggacagtg tgttaagtta cacttgggag
    14521 taaaaaagag gaggaccaag tggataattt aaggaggaag cacacgccgt cattta
//
LOCUS       DAOSMF010000036        14314 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_4969_length_14314_cov_26.136265, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000036 DAOSMF010000000
VERSION     DAOSMF010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 14314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14314
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4969_length_14314_cov_26.136265"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="PLT63_05515"
     CDS             <1..620
                     /locus_tag="PLT63_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98218.1"
                     /translation="LTAVRYVAATHFHIDHIGGMGPLLAACGPETRLLFPPPVHDYLT
                     GRRRLNRMAGWLSALPPVTLAAARGLRRLEDALPATLAGIPLPLLHRRTELSFPVERC
                     DFPRTGAGPPGFADWEILATPGHTEDSTSFYSRETAALICGDLILHMKQDGRGVLNAF
                     CWNRDVILDTLVRLRHTIAPRTVYPGHGEPWEGGERMLDNIVKQW"
     gene            684..3437
                     /locus_tag="PLT63_05520"
     CDS             684..3437
                     /locus_tag="PLT63_05520"
                     /inference="COORDINATES: protein motif:HMM:NF017459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF799 family lipoprotein"
                     /protein_id="HOD98219.1"
                     /translation="MKRYPHILLLAALAWGALLLAGCTVATKETVITPQLREAFEGKY
                     QVYPALAKAKPLSVAVLPFTDVSRSRVGTQEVRRGFYNHFSSLPFRDMELWRVDNLLK
                     KAGLTDLELINKTPARDLGKILGTDAVVYGEISNFDKLFAVVYSQVSVGAKLRMYDAR
                     SGELLWTGEHVARIHEGGIAVNPVGLVATVVATAMNVRDVQLLRACDDLFRDMVKTIP
                     APTIAEAKRPPVITLLVQDTKNLPKKAGDEIRVVIQGTPKMQASFAIGTYRRHIEMQE
                     QREEPGVYLGVYKVVPGDEVRKAVITGSLTDDAGNAAHWVDAVGTVTLKTTPPAPPQA
                     MRIVGRDAAVNVSWEKSADPELAGYRVYRSLTPLSGHQEVARTELTEYRDEKLVNGRT
                     YYYSVTAVDLAGNESDRTAGAGVPVAPGPTAVGGELEKDTVWHAGASPYIIERPLLVK
                     DKTTLTIEAGTEIRSAGPGLTVAGGLIAAGDGERMISWDVATPGKAWEGITLRNVQDR
                     EIILRHNRIRNAAVGLNLQSSSPKIEACELTENGTAVRISGSFAKPRVAGSAIRKSAA
                     AAIIVTEGSQPLLTENRIQDNLKQGIFVEGAAPTISRNAIARNGAGGIEIRNGHAVIT
                     ENNFTDNHPCHIAGAMTGAPVSAFDNWWGTAKGPEILASLRGRIDIGSFLDAPYPEGK
                     RTVLPILGPTLGGTLTADAYLTLSNSPYRVAKNLVVDGGATLFVQPGVTLRFDQKTAL
                     AVEDGGIMAKGTPEHPIVFTATAATPAPGFYAGAISWRKKTAVNSALHYAIVQYADIA
                     LDIAYGAPEITSCLIARSAQSGIYCRNDAAPAVSLCTLTENRGEGAITVVGNARPRFH
                     HNNIFNNDFAAQARSTIYIDARNNWWGKDPPDPRMIMGDTASNINIEPWLPAPEAGAF
                     AGK"
     gene            3621..4517
                     /locus_tag="PLT63_05525"
     CDS             3621..4517
                     /locus_tag="PLT63_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HOD98220.1"
                     /translation="MHSNRRVTVGIIVATVFLFAGATAFCQTGGDGKVSMTEWTKIIE
                     TKVAEQAGDKGIINYEDGYVEAIGIGAPPERYLGKPNARPMALRAAKLDAYRNILEVI
                     KGVRVDSNTVVRDFMTENDEIRTSVEGVVRNFTVVNQDYMSDGTIEVTVRMNLSGRLS
                     QTVLPKGPEPEPSAAPAPAPAAPTTDSVTGLVIDARGLAARPAMSPKIVDENGKEVYG
                     SMQVDRQYAIQQGMTGYARDLTAAQSNPRVTSNPLSVKGIRADGPGKCDIIISNADAA
                     KVRASAEAMNYLQKCRVMIVLD"
     gene            4629..6671
                     /gene="mutL"
                     /locus_tag="PLT63_05530"
     CDS             4629..6671
                     /gene="mutL"
                     /locus_tag="PLT63_05530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOD98221.1"
                     /translation="MNPDPKRNDVQNSVKLNNVHKISILPTELAHRIAAGEVIERPAS
                     ILKELLENSLDAGATVLRIELIKGGCAALRITDNGAGMEPADVPLAFQRHATSKLTVF
                     EDLYRLRTFGFRGEALPSIAAVARVELTTRPPASLAGTTIIVENSRVEELREAGCPAG
                     TTIHVSRIFDAVPVRKKFLKSEATEQGACLEAINRIALASPGVKIRVLSNGRPLWDMP
                     ATADAGERIGMVLGREAGSGLLKIKGGREELALVGYISRPEVTRANSKGIYCFINGRC
                     IRDALLNHAIMTAYRNVMEAKRYPAAVIFLTMPPEDVDVNVHPAKLEVRFRNPRAVYG
                     LVVEAILAGLAAVAPAADDMTAPAPTGTAGTNLTSQVYQRRLGEALKRYTIFGGTKKA
                     SFRPGTSIFARGGGLFDTPSTAGTRAGRHRDGAAGSGTDLPPFLGAASGSDHERRDEP
                     GSFSDHIAAAVAPTGPGDPHGFLSPGIFPSGDGGPKRLFAAMTYLGQTGGCYLVFSHH
                     DELVLVDQHAAHERILYEEFRRGGREGRVAVQPLLLPEVATLSPGDYGVLMEHRELLR
                     EAGLEIEPFGGHTVSIRSLPAVLGHLDPRTLLEESLAQLTGAAGAVSWQEKRDKVLIS
                     LACKGAVKAGQVLSEPEVVRLCQDLDRLPFAGTCPHGRPVWIVLTARQLAGLFRRT"
     gene            6750..7691
                     /gene="miaA"
                     /locus_tag="PLT63_05535"
     CDS             6750..7691
                     /gene="miaA"
                     /locus_tag="PLT63_05535"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOD98222.1"
                     /translation="MKPSGEKRDQPRLVVIAGPTGVGKTALALALAKEFAAEIISADS
                     MQVYRYLDIGTAKPTAAERRLVPHHLLDCVDPDEEYHAARFRDEARQVIAALHGRGKN
                     IFVVGGTGLYIRVLLGGLLPGPGADGALRNHYRDLAARRGREHLHDLLRHRDPAAAGA
                     IAVNDTVRVIRALEYLKATGCSITAGQAAHRFGENRYKLLRIGLQAGREELFELIARR
                     ADAMLAAGLVDETRAIMARGYSGEIKALQSLGYKHIRAYLEGRCSLAEAREMMIRDTR
                     HYAKRQLTWFRGEPDMRWHGRGEEDRIAALIKEFLAP"
     gene            8001..9203
                     /locus_tag="PLT63_05540"
     CDS             8001..9203
                     /locus_tag="PLT63_05540"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOD98223.1"
                     /translation="MKRVNGCRRLFVFVAILAAVFLTISCGPKAVKPVGEMDTPDHHV
                     STGIKLLDQGQYPQAQGEFDRAIQLAPKFSRAFTGRALVKAYQNDFTGAFAALDKAWA
                     YADTKDEKLFIHIGYIRVNTMSKATCAKIGTDCSQDTTWLDKSKDEFDHAVAINAKSA
                     AAHYFMGLCYKTALDFPKAGQMFAKVLEIQSEYTAEADGQWKLVQDIQRAMPGTITGK
                     KIALVEKLSRADAAALFMEELKIDVLYKKRTPKDFDTSFKDPARAQSPSRGVASANDI
                     AVHPLKMDIEGILQLGVRGLENYPDGNFHPDESVNRAAYALMVEDILIKVTGDNSLAT
                     KFIGGTSTFPDLRNDLPYFNAVMVVTSRGIMQAKSISTGEFAPLDSVSGVEALLSIKK
                     FKEELKIF"
     gene            9217..11877
                     /locus_tag="PLT63_05545"
     CDS             9217..11877
                     /locus_tag="PLT63_05545"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOD98224.1"
                     /translation="MGRGQLSKVFEPHAAEEKWYRYWQDNDFFHAIDGSDKPQFSIVI
                     PPPNVTGMLHMGHALNNALQDIMIRYRRMQGYNAMWMPGTDHAGIATQNVVEQELAKE
                     GLTRHDLGREKFIARVWEWKEKYGGVITNQLKRLGCSCDWQRERFTMDAGLSQAVREV
                     FVRLYNDGLIYQGDYIVNWCPRCHTAISDLEVEFVEEAGHLWDIRYPYADGSGEIIVA
                     TTRPETMLGDTAVAVNPNDPRYKDKIGKTVILPLVNREIPIIGDDYVTMDFGSGAVKI
                     TPSCDPADFAMADRHNLEIIKIMDGSAVINENGGVYQGQDRFECRANVVKDLEKGGFL
                     GNIEPYTHNVGHCYRCKTTIEPAVSKQWFVKVAPLAKAATAAVIKGRTRIIPAMWEAT
                     YFEWMTNIRDWCISRQIWWGHRIPVWYCDGCGNMIVSAQDPSHCPACKGSELRQEEDV
                     LDTWFSSALWPFSTLGWPEQTPALKTFYPTSLLVTGFDILFFWVARMMMMGLYTMKDV
                     PFRDVYLHALVRDEHGDKMSKSKGNIIDPLEMIDKFGTDAFRFTLAAFTAQGRDVRMS
                     EERIEGYKFFVNKIWNATRFVLMNIEDYPDGEPRVEAAARSLPDRWIESRLRRTIAEV
                     IKGLDEYKFNEATGSIYRFIWHEYCDWYLELAKPVLYGREDSAARLAAQDTLYRVLRT
                     VMKLLHPTMPFLTEEIWQTIVNDGSSIMVSPFPTPEEYADDPRAEAEMELIMEAITKI
                     RTIRGEMGIAPAKKIRAILSAPDESTLAILAAGARYIAGLAGLENLETGVGLAEPKGA
                     ATGVVGSVQVFVLLEGAIDVTAEQARLEKELAKLDKELAIVQRKLANSDFRAKAAAAV
                     VAKEEEKDRELREKHLLLEKALKKVRELAS"
     gene            11997..12839
                     /gene="nadC"
                     /locus_tag="PLT63_05550"
     CDS             11997..12839
                     /gene="nadC"
                     /locus_tag="PLT63_05550"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOD98225.1"
                     /translation="MDHYENTLRQRLISAALAEDIGPGDVTCNAVLTGDETGSARAIA
                     KAGLVVAGLDTFRETFLAVDPELSFTALTAEGEEVVPGAKLAEIAGSLKSILMAERTA
                     LNLFQRMCGIATTTRAFVRTVAGTGAKILDTRKTAPGLRILDKYAVRVGGGTNHRYAL
                     FDGVLIKDNHIAAAGGIAPAIAMARRRITHTLKIEIEVKSIAELTEALAAGADTVMLD
                     NMSVADMKTAVAMTAGRASLEASGNVTLKTVRRIAETGVDFISVGALTHSVQAADISL
                     LIAQ"
     gene            12911..13732
                     /locus_tag="PLT63_05555"
     CDS             12911..13732
                     /locus_tag="PLT63_05555"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOD98226.1"
                     /translation="MERIIRTPVATPLSEERLAAVMAGMKLGFPVRYFPEIDSTNLQA
                     WRLAGEGAPEGTTVVADTQIRGRGRLNRSWVSPPGANIYASVVLRPRIDPAAAPQLTL
                     LAGVAVAELLSGRLPTGVKIKWPNDILIAGKKVCGILTEMRATAAAIDFIILGIGINV
                     NMDREDFEPSLQETATSLKIAAGASFDRLKVISALFSCLEKWYWIFLARGFPGVREAW
                     LRHADIVGRPIRVVFKKDAQTGVVTGIDADGTLVMKDTEGIVRRVIAGDIHLLKE"
     gene            13737..>14314
                     /locus_tag="PLT63_05560"
     CDS             13737..>14314
                     /locus_tag="PLT63_05560"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOD98227.1"
                     /translation="MLLVVDVGNTNTVLGLFDGDTLVHDWRIRTVVDHTVDEYGMLIY
                     NLYKSSKIGAKRISDIIISCVVPPMLNILEPLCRKYFHLKPLIVGPGVKTGMPIYYDN
                     PKEVGADRIVNAVAAFEKYRRDLIIVDFGTATTFDYVSAKGEYMGGCIAPGIMISSEA
                     LFERAAKLPRVELSKPKSIIAKDTVSSMQAGI"
BASE COUNT         2890 a         4606 c         4443 g         2375 t
ORIGIN      
        1 ccctgacggc ggtgcgctat gtggccgcca cccactttca catcgaccac atcggcggga
       61 tggggccgct gctggcggcc tgtggaccgg aaacccggct cctctttcct cccccggtcc
      121 atgactacct caccggacgg cggcgattga accgtatggc cggctggttg tccgccctcc
      181 cccccgtaac cctggccgcc gcccggggcc tgcgccgcct cgaggacgcc ttgccggcca
      241 ctctcgccgg catccccctg ccgctcctgc accgccggac cgaattatcc tttcccgtgg
      301 aaagatgcga tttcccgagg acgggagcgg gtccgccggg ctttgcggac tgggagatcc
      361 tggccacgcc ggggcacacg gaggattcga cgtccttcta ctcccgggaa acggcggcgc
      421 tgatctgcgg cgatttgatt ctccacatga agcaggacgg ccgcggcgtc ctgaacgcct
      481 tctgctggaa ccgcgacgtc atcctggaca ccctcgtccg cctgcgccac accatcgcac
      541 cgcggaccgt ttatccgggg cacggcgaac cctgggaagg aggagagcga atgttggata
      601 atatagtgaa gcagtggtag aatcggaccg gcggccaagg cgccggatgg acgacacatc
      661 gatgcaggga ggatcaagtc cagatgaaaa gatacccgca tatcttgctg ctggcggcgt
      721 tggcgtgggg agcgttgctc cttgccggct gcacggtggc caccaaggaa acggtcatca
      781 ccccccagct ccgggaggcc ttcgaaggca aataccaggt ctatcccgcc ctggccaagg
      841 ccaaacccct gtccgtagcc gtcctgccct ttaccgacgt ctcccggagc cgggtgggaa
      901 cccaggaggt gcgcaggggc ttttacaatc atttcagttc cctgcccttc cgggacatgg
      961 aactctggcg ggtggacaac ctgctcaaaa aggcggggct gacggatctc gagctcatca
     1021 ataagacccc ggcccgagac ctgggcaaga tcctgggaac cgacgccgtg gtttacgggg
     1081 agatatccaa tttcgacaag ctcttcgccg tcgtttactc ccaggtctcc gtcggggcca
     1141 aactccggat gtacgacgcc cgctcggggg aactgctttg gaccggggag catgtggccc
     1201 ggatccacga gggggggata gcggtgaatc ccgtggggct ggtcgccacc gtcgtcgcca
     1261 ccgccatgaa cgtccgggac gtccaactgc ttcgggcctg cgatgatctc ttccgggaca
     1321 tggtcaagac gatccccgcc cccaccatcg ccgaggccaa gcggccgccc gtcatcaccc
     1381 tcctggtcca ggacaccaag aatcttccga agaaggccgg ggacgagatc cgggtcgtca
     1441 ttcaggggac gccgaagatg caggcctcct tcgcgatcgg gacctaccgg cggcatatcg
     1501 agatgcagga acagcgggaa gaaccgggtg tctatctggg ggtttacaag gtggttccag
     1561 gtgacgaggt acggaaggcg gtcatcacgg gctcccttac cgatgacgcc ggcaacgcgg
     1621 cccactgggt ggatgccgtg ggtacggtga ccctgaagac caccccgccc gcgcctcccc
     1681 aggccatgcg gatcgtcggc cgcgacgccg ccgtcaacgt ctcctgggag aaatccgccg
     1741 atccagagct ggcggggtac cgggtttacc ggagcctgac gccgctcagc ggccaccagg
     1801 aggtcgcccg cacggaactg acggaatacc gggacgaaaa gctcgtcaac ggccggacct
     1861 actattactc ggtaaccgcc gtggatctcg ccggcaacga gagcgaccgg acggcgggcg
     1921 ccggcgtccc cgtcgccccg ggccccaccg ccgtaggcgg ggagttggaa aaggacaccg
     1981 tctggcatgc cggcgccagt ccctacatca tcgaaagacc gttactggta aaagacaaaa
     2041 ccaccctgac tatcgaggcg ggcacggaga tccgttcggc gggaccgggt cttaccgtag
     2101 cgggggggct gatcgccgcc ggggatgggg agcggatgat ttcctgggat gtcgcgacgc
     2161 cgggcaaggc ctgggaaggg atcaccctcc gcaacgtcca ggaccgggag attatccttc
     2221 ggcacaaccg gattcggaac gccgcggtgg gcctgaatct tcaatcctcg tcgccaaaga
     2281 tcgaagcctg tgaactgacg gaaaacggca cggcggtccg gatatccggg agtttcgcca
     2341 agcctcgggt ggcgggcagc gcgatcagaa aaagcgccgc cgccgcgatc atcgtcacgg
     2401 aaggctccca gccgctgctg acggaaaacc ggattcagga caatctgaag caggggattt
     2461 tcgtggaagg ggccgctccg accatttccc ggaacgccat cgcccgcaac ggcgccggcg
     2521 gcatcgaaat ccggaacggc cacgccgtaa ttacggaaaa caatttcacc gacaaccacc
     2581 cctgtcacat cgcgggggcc atgaccgggg cccccgtctc tgccttcgac aactggtggg
     2641 gcacggcaaa aggtccggaa atcctcgcct ccctccgggg aagaatcgat atcggctcct
     2701 tcctcgacgc cccctatccg gagggaaaac ggaccgtcct gcccattctc ggcccgactt
     2761 tgggcgggac cctcacggcg gacgcctatc tgaccctgag caacagtccc taccgcgtgg
     2821 cgaagaatct ggtcgttgac ggcggggcca ccctgtttgt ccaaccgggg gtgacgctcc
     2881 gtttcgacca gaaaacggcc ttagcggtgg aagacggcgg catcatggcc aaggggacgc
     2941 cggaacatcc catcgtcttt accgccaccg ccgccacccc ggcgccgggt ttctatgccg
     3001 gcgccatcag ttggcggaaa aagacggccg tcaactccgc cctgcattac gcgatcgtac
     3061 aatatgccga tattgccctg gatatcgctt acggagcgcc ggagataacc tcctgcctca
     3121 tcgccaggag cgcccagagc ggcatctatt gccgcaacga cgccgcgccc gccgtctccc
     3181 tctgcaccct gacggaaaac cggggggaag gggcgatcac cgtcgtaggc aacgcccgtc
     3241 cccggttcca ccacaacaac atcttcaaca atgatttcgc cgcccaggcc cgctcgacga
     3301 tctatatcga cgcccggaac aactggtggg gcaaggatcc tcccgatccg cgcatgatca
     3361 tgggggacac ggccagcaat atcaacatcg agccctggct cccggccccg gaggccgggg
     3421 cctttgccgg gaaatagaga aaaaaggccc tttttccgtc acaaagaggg atggaagcgg
     3481 cccccacgga taaaataaac cgttgcatcc cccgggggca tttgttaaca tggcgcccga
     3541 tttagaggca aaaaaacgat agaatcagca tccggccgga aatcaaagcg agttggccgg
     3601 taaaacggaa ggaggacgat atgcacagca accggcgcgt taccgtaggg atcatcgttg
     3661 cgaccgtatt tttgttcgcg ggggccacgg cgttctgtca gaccggcggc gatggcaagg
     3721 tcagcatgac ggaatggacg aaaatcatcg aaacgaaggt cgccgaacag gccggcgaca
     3781 agggcatcat caactacgag gacggatatg tggaggccat cggcatcggc gcccccccgg
     3841 aacgctatct ggggaagccc aacgcccgcc ccatggccct gcgggccgcc aagctggacg
     3901 cttaccgtaa tatcctggag gtaatcaagg gtgtgcgcgt cgattccaat accgtggtga
     3961 gagacttcat gacggaaaac gacgagattc ggacctccgt ggaaggggtg gtcagaaatt
     4021 tcactgtggt gaatcaggac tacatgtcgg acggtacgat agaggtgacg gtgcggatga
     4081 atctcagcgg ccgcctctcc cagacggtgc tgcccaaagg accggagccg gagccgtcgg
     4141 ccgctcctgc gcccgccccg gcggcgccga ccacggattc cgtcaccggc ctggtgatcg
     4201 acgcccgggg cctggccgcc cggccggcca tgtcccccaa gatcgtcgat gaaaacggca
     4261 aggaggttta cggctccatg caggtggacc gccagtacgc catccagcag ggcatgacgg
     4321 gttacgcccg ggatctgacg gcggcccaga gcaatccccg ggtcaccagc aaccccctga
     4381 gcgtcaaggg catccgggcc gacggtccgg gaaagtgcga catcatcatc agcaacgccg
     4441 acgccgccaa ggtgcgggcc tccgccgagg ccatgaacta cctgcagaag tgccgggtga
     4501 tgatcgtcct ggattgatcg tcccttccca ccggaaccac gaatcgatac cggccccatc
     4561 ctcccccgcc ggggagggga tggggctttt aaatccgctc aaggcagcgg tcaacggcct
     4621 ttgaatacat gaatcccgac ccgaaacgta atgatgttca aaattcagta aagctgaata
     4681 atgttcataa aatcagtatc ctgccgaccg agctggccca tcgcatcgcc gccggcgagg
     4741 tgatcgaacg tcccgcctcg atcctcaagg aactcctgga aaattccctg gatgccgggg
     4801 cgaccgtcct gcggatcgaa ctgatcaaag ggggctgcgc cgccctgcgc attaccgaca
     4861 acggggcggg catggaacca gcggatgtac ctctggcctt ccagcgtcac gccacgagca
     4921 aattgaccgt tttcgaagat ctctaccgac ttcggacctt cggcttccgg ggcgaggccc
     4981 tgcccagcat cgccgccgtg gcccgggtgg aactgaccac ccggccgccg gcctccctgg
     5041 cagggaccac gatcatcgtg gaaaacagcc gggttgagga gctccgggaa gcaggctgtc
     5101 ccgccgggac aacgattcat gtgagccgga tcttcgacgc cgtgccggtg cgcaaaaaat
     5161 tcctcaaatc ggaggcgacg gaacaggggg cctgtctgga ggcgatcaac cgcatcgccc
     5221 tcgcctcccc cggcgtcaag atccgtgtcc tgagcaacgg ccgccccctc tgggacatgc
     5281 cggccaccgc ggacgccggg gaacggatcg gcatggtcct cggccgggag gcagggagcg
     5341 gcctgctcaa gatcaaggga ggtcgggaag agctggcgct ggtcggttac atctcccgcc
     5401 cggaggtcac tcgggccaac tcgaaaggca tctactgttt catcaacggc cgctgcatcc
     5461 gcgacgccct tctcaatcat gccatcatga cggcctaccg gaacgtcatg gaggccaaac
     5521 gctacccggc ggcggtcatc ttcctgacga tgccgccgga ggacgtggac gtcaacgttc
     5581 atcccgcgaa actcgaggtt cgttttcgga atccccgggc cgtttacggc ctggtggtgg
     5641 aagccatcct ggcgggctta gcagccgtcg cccccgccgc tgacgacatg acggcccccg
     5701 cccccacggg caccgcgggt accaacctga catcacaggt ctatcagaga cgtctcggag
     5761 aggcgctgaa acgctacacc attttcggag ggacgaaaaa ggcctccttt cggcccggaa
     5821 catcgatctt cgccagaggc gggggcctct tcgacacccc ctccaccgct ggcacccgcg
     5881 ccgggcgaca cagggacggg gccgccggat cgggaacgga cctgccgcct tttcttgggg
     5941 ccgcaagtgg tagcgatcat gaacggcggg atgagccagg gtctttttcg gatcacattg
     6001 ccgcggcggt ggctccgacc ggccccggcg atccgcacgg tttcctgtcc ccgggtattt
     6061 ttccctccgg agacggaggc cccaaacggc ttttcgccgc catgacctat ctgggacaga
     6121 ccgggggctg ctatctcgtc ttctcccatc acgacgagct ggttctggtg gatcagcatg
     6181 ccgcccatga gcgcatcctc tacgaggaat tccgccgggg cggccgggag ggccgggtag
     6241 ccgtccagcc cctgctcctc cccgaggtgg ccaccctttc cccgggagac tacggggtcc
     6301 tcatggaaca tcgggaactg cttcgggagg cgggactgga gatcgagccc ttcggcggcc
     6361 ataccgtctc catccggtcc ctgccggcag tcctcgggca cctggatcca cggacgctcc
     6421 tggaggaatc cctggcacaa ctgaccgggg cggccggggc ggtctcctgg caggagaagc
     6481 gggacaaggt gttgatttcc ctggcctgta agggagcagt caaggcggga caggtccttt
     6541 ccgaaccgga ggtggtccgc ctctgtcagg atctggaccg cctgcctttc gcgggtacct
     6601 gcccccacgg ccgtccggtc tggatcgtcc tgacggcgag gcaactggcc ggcctcttca
     6661 ggcggacata aaatgcaaag aaccatgaac acaccccccg tccgggggac agtgccgccg
     6721 ggcaggacaa tcaccttccg gaaccggaca tgaaaccctc aggcgaaaag agggatcagc
     6781 ctcggctggt ggtcatcgcc ggccccacgg gggtggggaa aacggccctg gccctggccc
     6841 tggccaaaga gtttgccgcc gagatcatca gcgccgattc gatgcaggtc tatcgctacc
     6901 tggacatcgg cacggccaag cccaccgcgg cggaacggcg cctggtcccg caccatctcc
     6961 tcgattgcgt cgatcccgac gaagaatacc acgccgcccg gttccgtgac gaggcgcggc
     7021 aggttatcgc cgccctgcac ggacggggga agaacatctt tgtcgtcggc ggcacgggcc
     7081 tgtacattcg ggtcctgctc ggggggctcc tgccgggtcc gggcgccgac ggggcgttga
     7141 ggaatcatta ccgggatctg gccgctcgca ggggccggga gcacctccac gacctcctgc
     7201 gccatcggga cccggcggcg gcgggcgcca ttgcggtcaa cgatacggtg cgggtcatcc
     7261 gggccctgga gtacctaaaa gccaccggct gttccatcac cgccggtcag gccgcccacc
     7321 gcttcgggga aaatcgttac aagttgctcc gaatcggcct ccaggccggg cgggaagaac
     7381 tttttgaact tattgcccgc cgggccgatg ccatgcttgc cgccggtctc gtggacgaaa
     7441 cccgggcgat tatggcccgg ggatactccg gggagatcaa ggcactccag tccctgggct
     7501 acaagcacat ccgcgcctat ctggaggggc gctgctccct ggccgaggcc cgggaaatga
     7561 tgatccggga cacccggcat tacgcaaagc gacagctcac ctggttccgc ggggagccgg
     7621 acatgcggtg gcacggccgg ggcgaggagg accgcatcgc ggccctcatc aaggaattcc
     7681 tcgccccgtg agcctcccca cctgccggta aggcatttcc ttccctcccc ggggggacga
     7741 ggcgagggga gaagagggaa tttacatata tttatgaatg ttttcaacaa cggggcattg
     7801 ggaaattgct tgacataaaa aaacgctccc cctatatggt ggagacgcca cggactcgaa
     7861 caccctggac ggcttgacgt tgccggggga aaggaaggga agatccatgc cccttgatcc
     7921 ccgccgcacc gggggaagga tgggggcatc aatccgatcc cataaaatga ggatgttttt
     7981 caggtgaaag gagaagggat atgaagcgag tgaacggctg ccggcgattg ttcgtcttcg
     8041 tcgccatcct ggcggcggtt ttcctgacga tctcctgcgg gccgaaggcg gtcaagccgg
     8101 tgggagaaat ggacaccccc gatcatcacg tatccacggg cataaaactc ctggatcagg
     8161 gccagtatcc ccaggcccag ggcgagttcg accgggccat ccagttggcc ccgaagtttt
     8221 cccgggcctt caccggccgg gccctcgtca aggcctatca gaacgatttc accggggcct
     8281 tcgccgccct ggacaaggct tgggcatacg ccgacacgaa ggacgagaag ctcttcatcc
     8341 acatcggcta catccgggtc aacaccatga gcaaggccac ctgcgccaag atcggcaccg
     8401 actgctccca ggataccacc tggctcgaca aatccaaaga tgagttcgac cacgccgtgg
     8461 ccatcaacgc gaagtccgcc gccgcccatt acttcatggg cctctgctac aagaccgccc
     8521 tggatttccc caaggccggc cagatgttcg ccaaggtctt ggagatccag tctgaatata
     8581 ccgcggaggc cgacggacag tggaaactcg tccaggatat tcagcgggcc atgcccggca
     8641 cgattacggg caagaagatc gccctggtgg aaaaactgag ccgggccgac gcggcggcgc
     8701 tgtttatgga agagttgaag atcgatgtcc tctacaagaa acgaaccccg aaagacttcg
     8761 acacctcttt caaggatccc gcccgggccc agtcgccgag ccggggggta gcctcggcca
     8821 acgacatcgc cgtccatccc ctgaagatgg atatcgaagg catcctgcag ctcggcgtcc
     8881 gggggctgga gaattacccc gacggtaatt tccatcccga cgagtccgtc aaccgggccg
     8941 cctacgccct gatggtcgag gacatcctca tcaaggtcac cggggacaac agcctggcca
     9001 ccaagtttat cggcggcacc tccaccttcc cggacctgcg gaacgacctg ccctacttca
     9061 acgccgtcat ggtggtgacc tcccggggca tcatgcaggc caaaagcatc tcgacgggag
     9121 aattcgcccc cctggattcc gtctcgggcg tggaggccct gctgagcatc aagaagttca
     9181 aagaggaact gaagatattc taaaaggaga gatttcatgg gcagagggca gttgagcaag
     9241 gttttcgagc cgcatgcggc cgaagagaag tggtatcgat actggcagga caacgacttt
     9301 ttccacgcca tcgacggcag tgacaagccg cagttttcca tcgtcattcc gcctcccaat
     9361 gttacgggca tgctgcacat gggccatgcc ctcaacaacg cccttcagga catcatgatc
     9421 cgctaccggc ggatgcaggg ctacaacgcc atgtggatgc ccggaacgga ccatgccggc
     9481 atcgccaccc agaacgtggt ggagcaggaa ttggccaagg aaggcctgac ccgccatgat
     9541 ctcggccggg agaagttcat cgcccgggtc tgggagtgga aggaaaaata cggcggcgtc
     9601 atcaccaacc agctcaagcg cctgggttgc tcctgcgact ggcagcggga gcggttcacc
     9661 atggacgccg gtctctccca ggccgtccgg gaggtcttcg tccgtctgta caacgacggg
     9721 ctgatctatc agggtgacta catcgtcaac tggtgccccc ggtgccatac cgccatatcc
     9781 gatctggagg tcgaattcgt ggaagaggcg ggtcatctct gggacatccg ctatccctac
     9841 gcggacggca gcggcgagat catcgtcgcc accacccgcc cggaaaccat gctgggggac
     9901 acggcggtgg cggtcaaccc caacgacccc cggtataagg acaagatcgg caagacggtg
     9961 atcctccccc tggtcaaccg ggagatcccc atcatcggcg acgattacgt caccatggat
    10021 ttcggctccg gggcggtgaa gatcaccccc tcctgcgatc ccgccgactt cgccatggcg
    10081 gaccgccaca acctggagat catcaagatc atggacggca gcgccgtgat caacgaaaac
    10141 ggcggcgtct atcagggcca ggaccggttc gaatgccggg ccaatgtcgt gaaggatctg
    10201 gagaagggcg gcttcctggg caacattgaa ccttacaccc ataacgtggg ccattgctac
    10261 cgctgcaaga ccaccatcga acccgccgtc tcgaagcagt ggttcgtcaa ggtcgccccg
    10321 ctcgccaagg cggccacggc ggcggtcatc aagggcagaa cccggatcat ccccgccatg
    10381 tgggaggcca cctacttcga atggatgacc aacatccgcg actggtgcat ctcccggcag
    10441 atctggtggg ggcaccggat ccccgtgtgg tattgcgacg gctgcggcaa catgatcgtg
    10501 tccgcccagg acccctccca ctgtccggcc tgcaagggct ccgaactccg ccaggaggaa
    10561 gacgtcctgg atacgtggtt cagctccgcc ctctggcctt tctccaccct gggctggccg
    10621 gagcagaccc cggcgctgaa gaccttctac cccacctcgc tgctcgtcac cggcttcgac
    10681 atcctcttct tctgggtagc ccggatgatg atgatgggcc tgtacaccat gaaggacgtg
    10741 cccttccggg atgtctatct ccacgccctg gtccgggacg agcatgggga caagatgagc
    10801 aaatccaagg gaaacattat cgatcccctg gaaatgatag ataaattcgg caccgacgcc
    10861 ttccggttca ccctggccgc cttcaccgcc cagggccggg atgtccggat gtcggaagag
    10921 cggatcgagg gctacaaatt cttcgtcaac aagatctgga acgccacacg gtttgtcctg
    10981 atgaacatcg aggattatcc cgacggagaa ccccgggtgg aggccgccgc ccgttccctg
    11041 cccgatcgct ggatcgagtc gcggctccgc cggaccatcg ccgaggttat caagggacta
    11101 gacgagtaca agttcaacga ggctacgggg agcatctacc ggttcatctg gcatgagtac
    11161 tgcgactggt atctggaact ggccaaaccg gtcctctacg gccgcgagga ctccgccgcc
    11221 cgcctggccg cccaggatac cctgtaccgc gtcctgagga ccgtgatgaa gctcctccac
    11281 cccaccatgc cgttcctgac ggaggagatc tggcagacca tcgtgaacga cgggagttcc
    11341 atcatggtga gccccttccc caccccggag gaatatgccg acgatccccg ggcggaggcg
    11401 gagatggagc tgatcatgga ggccatcacg aagatccgga ccatccgcgg ggagatgggg
    11461 atcgcccccg ccaagaagat ccgggccatc ctctcggcgc ccgacgagag caccctggcg
    11521 atcctggccg ccggggcgcg ttacatcgcc ggcctcgccg gcctggaaaa tctcgaaacc
    11581 ggcgtcggtc tggccgaacc caagggggcg gccacgggcg tagtgggcag cgtgcaggtc
    11641 ttcgtcctcc tggagggggc catcgacgtc accgcggaac aggcccgcct ggaaaaagag
    11701 ctggccaaac tggacaagga gctggccatc gtgcaacgga agctcgccaa cagcgatttt
    11761 cgcgccaagg cggccgccgc cgtagtggcc aaggaagagg aaaaggaccg ggaactccgg
    11821 gagaagcacc tcctcctcga aaaggccctg aagaaggtcc gggagctggc gtcctgaggc
    11881 tggtatgaca ctccttcccc ttcaaggggg aggaatagaa tcgatttccg agcgtgaggg
    11941 acaaaatcgg ttctagggat gaggggataa aatccgttgt tacgaggtcg tcaactttgg
    12001 accattatga aaataccctt cggcagcgac tgatcagcgc cgccctggcc gaggatatcg
    12061 gcccgggaga cgtgacgtgc aacgccgtcc tgactgggga tgagacggga agcgcccggg
    12121 ccatcgccaa ggccggcctg gttgtcgccg gcctggacac cttccgggag acctttctgg
    12181 cggtcgatcc cgagctctct ttcaccgccc taaccgcgga aggggaagaa gtcgttccgg
    12241 gcgcgaaact ggcggagatc gccgggtccc tcaaatcgat cctcatggcg gagcggaccg
    12301 ctctgaacct cttccagcgc atgtgcggca tcgccacgac gacgcgggcc tttgtccgga
    12361 ccgtggccgg gaccggcgcg aagatcctcg acacccgcaa gacggcgccg gggctacgga
    12421 tcctggacaa gtacgccgta cgggtagggg gcgggaccaa ccatcgctac gccctcttcg
    12481 acggggtgct catcaaggac aaccacatcg ccgccgccgg cggcattgcc cccgccatcg
    12541 ccatggccag gcgccgtatc acccacaccc tgaagatcga aatcgaagtg aagagtatcg
    12601 cggagcttac ggaagccctg gccgccgggg ccgataccgt aatgctggac aacatgagcg
    12661 tcgcggacat gaaaaccgcg gtggccatga ccgccggccg ggcgtccttg gaggcctcgg
    12721 ggaacgtgac cctgaagacc gtgcgccgca tcgccgagac gggcgtggat ttcatctccg
    12781 tcggcgcctt gacccactcc gttcaggccg ccgacatatc tttattgata gcccaataaa
    12841 aacagatcat tactaactcg gagaagatga ccggccgccg gagcggcggg gaaacaactt
    12901 ggcgacaggc atggaaagga ttatacgcac cccggtagcg acgcccctct cggaagagcg
    12961 gcttgcggcg gtcatggcgg gaatgaaact cggcttcccg gtccggtatt tcccggaaat
    13021 cgactccacc aacctccagg cctggcgtct ggccggggag ggcgccccgg aagggaccac
    13081 agtcgtggcg gacacccaga tccgggggag ggggcgtctg aaccggtcct gggtttcacc
    13141 cccaggggcc aatatctacg cctccgtggt cctgagaccc cggatcgacc ccgccgccgc
    13201 cccccagctg accctcctgg ccggggtggc cgtggcggaa ctcctgtccg gccggctccc
    13261 caccggggtg aagatcaaat ggcccaacga catcctcatc gcggggaaaa aggtctgcgg
    13321 gatcctcacg gagatgagag ccaccgcggc ggccatcgat ttcatcatcc tgggcatcgg
    13381 catcaatgtc aacatggacc gggaagactt cgaaccctcc ctccaagaga cggccacctc
    13441 cctgaagatc gccgccgggg cgtccttcga ccgcctaaaa gtaatatccg cgcttttttc
    13501 ctgcttggaa aaatggtatt ggatcttcct cgcccggggt tttccggggg tgcgggaggc
    13561 ctggctgcgc catgccgata tcgtgggccg gccgatccgg gtggtcttca agaaagacgc
    13621 gcaaaccgga gtggtcacgg ggatcgacgc cgatgggacc ctggtaatga aagacacgga
    13681 ggggattgtc cggcgggtga tcgccggcga tatccatctg ttgaaggagt gagatcatgc
    13741 tgctggtggt ggatgtggga aataccaata cggtgctcgg ccttttcgac ggggacaccc
    13801 tcgtccatga ctggcggatc agaacggtgg tcgatcatac ggtggacgag tacgggatgc
    13861 tgatctacaa cctctacaaa tcgagcaaga tcggcgccaa gcggatctcc gacatcatca
    13921 tctcctgcgt ggtccccccc atgctcaaca tcctggagcc cctgtgccgg aaatacttcc
    13981 atctcaagcc cctcatcgtc ggacccgggg tcaagacggg aatgcccatc tactacgaca
    14041 accccaagga ggtgggtgcg gaccggatcg tcaacgccgt ggcagccttc gagaaatacc
    14101 ggcgggatct gatcatcgtg gatttcggca cggcgacgac cttcgactac gtctcggcga
    14161 agggggaata catgggcggc tgcatcgccc cggggatcat gatctccagt gaggccctct
    14221 tcgagcgggc ggccaagctc ccccgggtgg agctgagcaa gcccaaatcc atcatcgcca
    14281 aggacacggt gagcagcatg caggcgggga tcat
//
LOCUS       DAOSMF010000037        14011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_5099_length_14011_cov_21.668100, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000037 DAOSMF010000000
VERSION     DAOSMF010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 14011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14011
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5099_length_14011_cov_21.668100"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            86..1795
                     /locus_tag="PLT63_05565"
     CDS             86..1795
                     /locus_tag="PLT63_05565"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOD98228.1"
                     /translation="MMRYSQMFLPTLKEMPSDAEVISHQLMLRAGMIRKLTSGVYSYL
                     PLGYRVIRKVEQIIREEMNRAGAQEIFMPTVQPAELWKESGRWSFYGKELLRFRDRKD
                     SECCLGPTHEEVVTDIVRNEVKSYRQMPLNLYQIQNKFRDEIRPRFGLMRCREFSMKD
                     AYSFDVDAERADESYQKMFEAYSRIFTRCGLKFRPVEADTGSIGGSYSHEFLVIADSG
                     EDAMAYCEGCGYAANMEKAEIARPETEDTQAPLLSLQEVYTPEVRTIEEVCAFLGVVP
                     EQVVKTLIFRADDVPVAVLVRGNEEVNEIKVKNFLGCEVLEMAMDDMIQAAAGSPRGF
                     VGPIGIKCRVLADYSLIGLHNMVVGANKENYHLKNVNFERDFQVAEFADLRVIRESDP
                     CPRCRREIRFARGIEVGHVFKLGTKYSKAMKAVYLDSRGQEQYMVMGCYGIGVGRTVA
                     ACIEQSHDGNGIIWPLAVAPFHVIITPVNMKENSLATAAEELYAELSAEGVDVLLDDR
                     DERAGVKFKDADLIGIPMRVTVGPKNLALGKVELKLRSTGETNVLSGSEAKAEIVAAV
                     HSL"
     gene            2017..4164
                     /locus_tag="PLT63_05570"
     CDS             2017..4164
                     /locus_tag="PLT63_05570"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HOD98229.1"
                     /translation="MLRITDILDKTQSYLIPADQEMIEKAYVFSATVHQGQMRLSGEP
                     YLTHPMEVAGMLADMKLDAATIITGLLHDTVEDTLTTIEQIEEAFGKDVAFLVDGLTK
                     ISKITFGSQEERQAENFRKMILAMSADLRILIVRLADRMHNMRTLNFQTLEKQRYIAT
                     ETLELYAPLANRLGINWMKVELEDLSFFYLDNDAYYEIARRIDKKKEQREQYTEEVRG
                     IIAKEITKYGLQGDVEGRAKHFYSIYAKMKNQRIDFDEVYDLIAFRIILDTDKDKECY
                     EALSIVHALWKPVPGRFKDRIAMPKANGYRSLHTTVIGPYGERVEIQIRTRQMHEWAE
                     EGIAAHWRYKERRADLGSDDQQIMKLRELLEVQQEVENPREFMANLKLALFPDEVYVF
                     TPGGDVKSFPKGATPLDFAYSIHTDVGNQCIGARVNRSIVPLKYELQNGDTVEIITQA
                     GHHPGKDWLKWAVTSRARSKIKNWIKTEERKRSIEIGREILEREFKKQNLKLSQFLKS
                     DDLKGIYEEQSISTPDDLMALIGYGRISAKHLVNRFVPEAAVKTEEPAADKIRKKAKS
                     VASLGISLTGIEDVLVKFAHCCDPIPGDEIVGYISRGRGITIHTSQCANLQELDPERF
                     VEVSWNIKDKQTYPVDILVICQDKKGVIAEVSSVISALDINISHAEVETTQDLRAICN
                     FRIEVHDLQEFNRVAAAVKKVKCVLSIERTKKS"
     gene            4234..4929
                     /locus_tag="PLT63_05575"
     CDS             4234..4929
                     /locus_tag="PLT63_05575"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOD98230.1"
                     /translation="MYRFRIVAVLTIICAVVLYGMPGLSRSQPERGDRAIVIDAGHGG
                     SDLGVRIADKTYEKDLNLRLALALQKELATSGYRRVLLTRSADKDVSVKERSELIRKT
                     NPALVISLHANAGFSGKARGYELYFPGFKTGKEDRNDPAAIIGDMTKNKHLNDSVRVA
                     QHIQKQLAEIFPKENRGLREAPLPLLEGINVPAVIIEIGFITNKENRKKLDDEKSRQG
                     IAQAIARGIREGI"
     gene            4930..5550
                     /locus_tag="PLT63_05580"
     CDS             4930..5550
                     /locus_tag="PLT63_05580"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: protein motif:HMM:NF011397.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HOD98231.1"
                     /translation="MAAPYHLVIASKNRGKVEELRAMLADLPLDILSLSDYPEAPAVF
                     EDGDSFLANALKKARSLAAYTGKTVLADDSGLEVDRLNGAPGIYSSRYAGEDATDDEN
                     NRKLLAEMRGVPGDERGAAFCCVLVLYRPDGTYEKFTGRWPGQIGETLRGRRGFGYDP
                     LFYVPALGKTVAELTPEEKNLASHRSQAVLALKKYLQDHVFPELRR"
     gene            5587..5663
                     /locus_tag="PLT63_05585"
     tRNA            5587..5663
                     /locus_tag="PLT63_05585"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5621..5623,aa:Pro,seq:tgg)
     gene            5710..6501
                     /locus_tag="PLT63_05590"
     CDS             5710..6501
                     /locus_tag="PLT63_05590"
                     /inference="COORDINATES: protein motif:HMM:NF005126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HOD98232.1"
                     /translation="MSEIKEGTISREKKGYVFLIGVHRATKMNAFTWDMLVDLSRAFG
                     EYEEDREMRCAVLYAEGDHFTAGLDLGDVAPHVAKGEPFIPEGAVDPWGVHGRMLSKP
                     LVIAVQGRCYTLGTELSLAADIVVATADTRFAQLEVKRGIFPFGGGTIRLAKTAGWGN
                     AMRYLLTGDEFDVQDAYRFGLVQEIVAPGQQFAKALEIAEKIASQAPLAVQDTLALAK
                     TATYEGFAAGVREIMPKLLKIMKTEDAAEGVMSLIERRPANFKGR"
     gene            complement(6513..7859)
                     /gene="trmFO"
                     /locus_tag="PLT63_05595"
     CDS             complement(6513..7859)
                     /gene="trmFO"
                     /locus_tag="PLT63_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HOD98233.1"
                     /translation="MPHRGQALIIGGGLAGCEAAWQLLCRGCRVTLWEMKPERYSPAH
                     SSPDLAELVCSNSLRSGDPASAAGLLKEEMRRLGSLFMAAADQTSLPAGKSLAVDRER
                     FSAYIQERLLAWGERFQLVRQELTELPTATPAIVATGPLTSDALAAAIQRLTGRECLY
                     FYDAIAPIVTADSVDPNRSFWASRYDPSSAENAGDYLNCPLDEETYRAFREEILTADK
                     FPLRSFENPRYFEGCLPVEILAQRGSRTLLFGPMKPVGLTDPRTGRQPWAVVQLRREN
                     ASGTLLNMVGFQTKLTRPEQERIFRMIPGLVQAEFARYGSIHRNTFLRSPGLINDRLQ
                     LISHPHLFFAGQLTGVEGYMESAAMGLYAGISLGRMLTGGELSPPPDTTALGSLIRHI
                     TTAGKNDFQPMNINHGLLRPLSLRIKKRERGREYARRSLADLSAWMTAQGIEVLPD"
     gene            complement(7849..10266)
                     /gene="topA"
                     /locus_tag="PLT63_05600"
     CDS             complement(7849..10266)
                     /gene="topA"
                     /locus_tag="PLT63_05600"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOD98234.1"
                     /translation="MPSSLVIVESPTKVKTIKKFLGPDFEVRATMGHVKDLPKSSLGI
                     DLERDFTPSYGVIDSKKKVIAEIKKAAQKAERIFLAPDPDREGEAIAWHVAEEIGSKK
                     RQLHRALFNDLTKKTVLDAIANPIALDRSKFEAQQTRRILDRLVGYKISPLLWEKVKR
                     GLSAGRVQSVAVRLICDREKEIGSFVSEEYWNITASLAAHMPPPFDARLVKIAGKKAR
                     IGDSEEANRVLRELGELDFVVTGEEKKEVKRNPLPPFTTSKLQQEASRWLRFSAKKTM
                     NVAQQLYEGVELGPEGSVGLITYMRTDSVRISEEALQEVRNYIRASYDPDFLPPKPRH
                     FKNSASAQDAHETIRPSSLQYRPGEIKSHLTPDQYKLYLLIWNRFVASQMSPARYDQT
                     VIDIAAGVYELRALGSILKFSGFSEVYREGKDDNGGEGEDEMGRLLPEVAIGEVLKLT
                     ALTPEQKFTQPPPRFSEATLVRELEEKGIGRPSTYAAILATIQDREYVRLEKGRFHPT
                     DLGMLVTELLVENFPKILDVSFTASLEDRLDRIEEGKNERIETLKDFYAEFARELQVA
                     AGKMKSLKGKGIPTDMVCEKCGSPMAIKWGRNGRFIACTNYPDCKNTMNIPEAGNGTE
                     GKNGEGVPELTCEKCGRTMVLKQGRFGKFMACSGYPECKTTVKITAAGGEPTPPKEEV
                     LTDKICEQCGKPLAIKSGRYGKFLGCTGYPACKVIHPLHLDIPCPEPGCNGFLSERRS
                     KTGKIFYGCTCYPECKFVTWSRPLPEACPLCNAPFLVEKRTKTGSYKQCHRKECKYRS
                     DLVVHAP"
     gene            complement(10271..11422)
                     /gene="dprA"
                     /locus_tag="PLT63_05605"
     CDS             complement(10271..11422)
                     /gene="dprA"
                     /locus_tag="PLT63_05605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOD98235.1"
                     /translation="MTQQPENMKYWLALRYVEGLGNIGVRNLLQSFGAPERIFRAPLN
                     MLAAVPGIGAKTARNINDFNLWERVAAELDRAAELPATIMTCQDPRFPRRLLFIYDFP
                     FLLYVQGELRPDDINIAMVGSRMASAYGKFTTERLSRELSLHGVTVVSGMARGIDTAA
                     HRGALSVKGRTIAVLGSGIDVIYPPENEGLARKIAQSGAVVSEFPLGTPPNATNFPAR
                     NRIISGMSLGVVVVEATEKSGSLITARIALEQGREVFAVPGGIDAAGSRGTNRLIKEG
                     AKLVENIHDILDEIKPQAPFSEDAPEEVKPLPPAATVFPPGRAPWRRPSISGKEGLVI
                     DHLSSHPRTVDDLIAATGLAASEVLPALLQLELRGLIRQTPGNKYILQE"
     gene            11867..13480
                     /locus_tag="PLT63_05610"
     CDS             11867..13480
                     /locus_tag="PLT63_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98236.1"
                     /translation="MLLAVVAIQPLAPVWSAEVLLHPEAIDLGDVGAGIFKKGQAIMT
                     FADAERKDWAIAAPEGWTCKAGQPLTGTIAHRHPAKINIAMKSSLAPVGENGRHQHLY
                     NVQLHLEFENMQVVYQRLIAAGPHREGIAFNIAGELKVIELSFHIREYPPEPVLAIRP
                     QRLDFGLLEPGRQVEGKIEITNRGRQPLKWKIEPTRWPADGENGEAAAGQYHSFHRPE
                     VTGTGAYRPDPTRSGAAEFKGNWTENEGYPEAQDKAVLKYNYSGTGIIVHFWKSPLAR
                     SMKVYLDNRPLGNLEAFGEQIAAAEYLLPETPAPGSHTLTLVNQQGRTVVEGVKILGR
                     EVVKDQGKWVRVFPDVGVTTREVDYLHIAVDTANLKPGAHGRCFRIDSNGGSAVVEIH
                     AEVASEAGAKILDVYRYRNGDDFLFTTNPQGEAGMLSARNYRKDGIAFRLFSPGTPGT
                     TEFYRWYNPRQQCHFYSYEAGGGEKLSRDYIMEGTIGNIATSRLPNTRPLHRWRHIGR
                     EQFFFTTDSRSEGYNRKGYRYDGIAGYVR"
     gene            13563..13639
                     /locus_tag="PLT63_05615"
     tRNA            13563..13639
                     /locus_tag="PLT63_05615"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13597..13599,aa:Arg,seq:tct)
     gene            13650..13726
                     /locus_tag="PLT63_05620"
     tRNA            13650..13726
                     /locus_tag="PLT63_05620"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13684..13686,aa:His,seq:gtg)
     gene            13744..13820
                     /locus_tag="PLT63_05625"
     tRNA            13744..13820
                     /locus_tag="PLT63_05625"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13778..13780,aa:Arg,seq:tcg)
     gene            13854..13929
                     /locus_tag="PLT63_05630"
     tRNA            13854..13929
                     /locus_tag="PLT63_05630"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13887..13889,aa:Lys,seq:ctt)
BASE COUNT         3086 a         3804 c         4201 g         2920 t
ORIGIN      
        1 agatcatcag aaaggtcaag gaaacggact tcgccaccga gctgctggcg gaaatacgat
       61 tgataataca ggaaaaaaag gaggcatgat gcgatactca caaatgtttc tgccgacgct
      121 caaggaaatg ccctctgacg cggaggtgat cagccatcaa ctcatgctgc gggccgggat
      181 gatccggaaa ctgaccagcg gagtatattc ctatctgcct ctgggctatc gggtcatcag
      241 aaaggtggaa cagatcatcc gggaagagat gaaccgagcc ggtgcccagg agatatttat
      301 gcccacggtc cagccggcgg agctgtggaa ggagtctggg cggtggagct tttacggcaa
      361 ggagcttctc cgtttccggg atcgcaagga ttcggaatgc tgcctgggcc ccacccatga
      421 agaagttgtt acggacatcg tccgtaacga ggtcaaatcc taccggcaga tgcctctaaa
      481 tctctatcag atccaaaaca aattccgcga cgagatccgc ccccgcttcg ggctgatgcg
      541 ctgccgggaa ttttccatga aggacgccta tagctttgac gtcgatgccg aacgggcgga
      601 tgaaagctac cagaagatgt tcgaagccta cagccgaatc tttacccggt gcgggctcaa
      661 attccggccg gtggaggccg acacggggag catcggcggc agttactctc atgaattcct
      721 ggttatcgcc gattcgggag aggatgccat ggcttactgc gaggggtgcg ggtacgcggc
      781 gaatatggag aaggcggaga tcgcccgacc cgaaacggaa gatacacagg cacctctttt
      841 atccttacag gaggtgtata ccccggaggt caggaccatt gaggaggtct gcgcctttct
      901 tggcgtcgtt cccgaacagg tggtcaaaac cctgatcttt agagccgacg atgtcccggt
      961 cgccgtcctg gtccgaggca atgaagaggt caacgagatc aaagtgaaaa atttcctcgg
     1021 ctgcgaagtc ctggaaatgg ccatggacga catgatccag gcggcggcgg gttccccccg
     1081 agggttcgtc ggccccatcg gtatcaaatg ccgcgttctg gcggattatt cactgatcgg
     1141 tctgcacaac atggtcgtgg gggccaacaa ggagaactat cacctgaaaa acgtcaactt
     1201 cgagcgggat ttccaggtgg cggagtttgc cgacttgcgt gtcatcaggg aatccgatcc
     1261 ctgtccgcgc tgccgccggg aaatccgttt cgcccggggc atcgaggtgg ggcatgtctt
     1321 caagcttggg acgaagtaca gcaaggccat gaaggccgtt tatctcgaca gccgggggca
     1381 ggaacagtac atggtcatgg gctgttacgg gattggcgtg ggacgcaccg tggcggcgtg
     1441 catcgaacag agccatgacg gcaacggcat catctggcct ctggccgtgg ccccctttca
     1501 cgtcatcatc accccggtga acatgaaaga aaattccctg gcgacggccg ccgaggaact
     1561 ttacgcggaa ctatcggcgg agggtgtaga tgtcctccta gacgaccggg acgaacgggc
     1621 cggggtgaag ttcaaggacg ctgaccttat cggcatccct atgcgggtga ccgtcggtcc
     1681 caaaaatctg gctctgggga aggtggaact caaactgaga agcaccgggg agacgaacgt
     1741 cctgtccggg agcgaggcga aggcggaaat cgtcgctgcg gtacattccc tctgatcacc
     1801 tccggtagga cggaagaatc atcggggtat ggaaccccct ccatgagccg aacggtgtgt
     1861 ttccaatagt tccacggggc gacttgacat ctcggcgttt atatcaaaat tatatgtcaa
     1921 gggtctgatg gggcgccgcc ttttcttatc tcgcctgacg gagaagcgct taccgttcgg
     1981 gaatcgttcg caaacacgga aattatcaac aatcatatgc tccgcataac cgacatcctc
     2041 gataaaaccc aatcctacct gataccagcg gatcaggaga tgatcgagaa ggcctacgtg
     2101 ttttccgcca ccgtccatca ggggcagatg cggctttccg gcgagcctta tcttacccat
     2161 cccatggagg tcgccggcat gcttgccgac atgaagctcg atgcggcgac catcattacc
     2221 gggctgctcc atgatacggt ggaagatacg ctgaccacca tcgagcagat cgaggaggct
     2281 ttcggcaagg atgtggcctt cctggtggac ggcctgacca agattagcaa gatcaccttc
     2341 ggttcccagg aggagcggca ggcggaaaac ttccggaaga tgatcctcgc catgtccgcc
     2401 gacctccgga tcctcattgt tcgcctcgcg gaccggatgc acaatatgcg gaccctgaat
     2461 ttccaaaccc tggaaaaaca acgctacatc gccaccgaaa ccctggaact ctacgcccca
     2521 ctggccaacc ggttgggaat caactggatg aaggtcgaac tggaagatct gtccttcttc
     2581 tatctcgaca acgacgccta ttatgaaatc gcccgccgga tcgacaagaa gaaggaacaa
     2641 cgggagcagt ataccgaaga ggtacggggg atcatcgcca aggaaatcac caagtacggc
     2701 cttcaggggg atgtggaggg aagggccaag cacttttaca gcatctacgc caagatgaag
     2761 aaccagcgga tcgatttcga cgaggtttat gaccttatcg ctttccggat catcctggac
     2821 accgacaagg acaaggagtg ctacgaggcc ctgagcatcg tccatgccct ctggaaacca
     2881 gttcccggcc gtttcaagga ccgtatcgcc atgcccaagg ccaatggcta ccggtcgctc
     2941 catacgacgg tcatcggtcc ctacggggag cgcgtggaga ttcagatccg gacccgccag
     3001 atgcacgaat gggcggaaga gggaatagcg gcccactggc gctacaagga gcgccgcgcg
     3061 gatctgggtt cggatgatca acagatcatg aaactccggg aacttctgga ggttcaacag
     3121 gaggtggaaa acccccgtga attcatggcc aatctcaaac tggccctctt tcccgacgag
     3181 gtttacgtct ttacaccggg tggcgacgtg aagtctttcc ccaaaggcgc cacacccctc
     3241 gatttcgcct acagcatcca taccgatgtg ggcaatcagt gcatcggcgc ccgggtgaac
     3301 cgaagcattg tgccgttgaa gtatgaactg caaaacggtg acaccgtgga gatcatcacc
     3361 caggcgggtc atcatcccgg gaaggactgg ctgaaatggg cggtgacctc ccgggcccgg
     3421 tccaaaatta agaactggat caagacggaa gaacggaagc ggagtattga gatcggccgg
     3481 gagatcctgg agcgggaatt caagaagcag aacctaaaat tatcgcagtt cctgaaatcc
     3541 gacgatctca agggcatcta tgaggagcag agcatctcca cgccagatga cttgatggcc
     3601 ctcatcggct acggtcgcat ttccgccaaa cacctcgtca accgctttgt cccggaagcg
     3661 gccgtcaaga ccgaagaacc ggccgcggac aagatccgaa agaaggcgaa aagcgtcgcc
     3721 tcgctgggca tctcccttac ggggatcgag gacgtcctag tgaagttcgc ccattgttgc
     3781 gatcccatcc ccggcgacga gatcgtggga tatatttccc gggggcgggg gattacgatt
     3841 cataccagcc aatgcgcgaa ccttcaggaa ctggaccccg aacgttttgt tgaggtgtct
     3901 tggaatataa aggacaaaca gacctatccc gtggatatcc tcgtcatctg ccaggacaaa
     3961 aagggggtga tcgccgaggt cagttccgtt atctccgccc tggatataaa catcagtcat
     4021 gccgaggtgg aaaccaccca ggatctgcgg gcgatctgca atttccgcat cgaagtccac
     4081 gatctccaag aattcaaccg ggtggcggcg gcagtcaaga aggtgaagtg tgtgctgtcc
     4141 atagaacgga cgaagaagtc atgagggagt cttccatgtt gacagccggg gataatatga
     4201 agtatgtacc gcccacgctt aaaggtggaa cccatgtata gattcaggat tgtcgctgtg
     4261 ttaacaataa tctgcgccgt cgtcctctac gggatgccgg gtttgtcccg atcgcagccg
     4321 gaacgcggcg accgggcgat tgtcattgac gccgggcatg gcggatcgga cttgggcgtc
     4381 aggatcgcgg acaagaccta cgagaaagat ctgaatctgc gcctcgccct ggccctgcag
     4441 aaggaattgg ccacgtcggg ctatcgccgg gtgttgctga cgagatccgc cgacaaggat
     4501 gtgtcggtga aagagcgaag cgaattgatc cggaaaacga atcccgccct ggtgatcagt
     4561 cttcatgcca atgccggttt cagcggtaag gcccggggat atgaactgta ttttccgggt
     4621 tttaaaaccg ggaaagagga ccgcaacgat ccggcggcga ttatcggtga tatgaccaaa
     4681 aacaagcatc taaacgacag cgtccgggta gcccagcata ttcagaagca gcttgcggag
     4741 atctttccta aggagaaccg ggggcttcgg gaggctcccc tgccgctgct ggaggggata
     4801 aatgtcccgg cggttatcat cgagatcggg tttattacca acaaggagaa ccgtaaaaag
     4861 cttgacgatg aaaaaagccg ccagggcatt gcccaggcca tcgcccgggg gattcgggaa
     4921 ggcatttaaa tggcggcccc atatcatttg gtcatcgcct ctaagaatcg gggcaaggtc
     4981 gaggagctca gggcgatgct cgccgatctg cccctggata tcctttccct gagtgattat
     5041 ccggaggcac cggcggtttt tgaagacggc gacagtttcc tggccaatgc tttgaaaaag
     5101 gcccgcagcc tggctgccta taccggcaag acggtcctgg ctgacgactc gggcctggag
     5161 gtggaccggc taaacggcgc cccagggata tattcgtccc ggtacgccgg ggaggacgcg
     5221 acggatgacg aaaacaaccg gaaactccta gccgagatgc ggggggttcc cggggacgaa
     5281 cggggtgccg ctttttgctg tgttctggtc ctttaccggc cggatggtac ttacgagaaa
     5341 tttaccggcc gctggcctgg gcaaatcggg gagacattgc ggggccgccg cggtttcggc
     5401 tacgatccgc tcttttatgt gcccgctttg ggaaagacgg tggcggaact gacaccggaa
     5461 gaaaaaaacc ttgccagcca ccggtcgcaa gccgtcctgg ccttgaaaaa atatttacag
     5521 gaccatgtct ttcccgaatt acgtcgttga gtgggaggca tcgggactcg ccattacatg
     5581 gatgtgcggg gcgtagcgca gcctggtagc gcgcctgctt tgggagcagg atgtcggggg
     5641 ttcaaatccc tccgccccga ccaatgataa tgccgtcacg gcgcagccaa tagaacaagg
     5701 agctaattaa tgagtgaaat aaaagaagga accatcagcc gggaaaagaa gggttatgta
     5761 tttttgatcg gcgtccatcg ggctacgaag atgaatgcct ttacctggga tatgctggtg
     5821 gatttgtccc gggcgttcgg ggaatacgag gaggaccggg aaatgcgctg cgccgttctg
     5881 tatgccgaag gcgaccattt cacggcaggt ctcgatctgg gcgatgtggc gcctcatgtg
     5941 gccaaggggg aacccttcat ccccgaagga gccgtggatc catggggggt ccatggacgt
     6001 atgctctcca agccgctggt gatcgccgtg cagggacgct gctataccct ggggacggaa
     6061 ctgtctctgg cggccgatat cgtcgtcgcc accgccgata cccgtttcgc ccaactggag
     6121 gtgaagcggg gaattttccc cttcgggggc gggacgatcc gcctagccaa gacggccggc
     6181 tggggtaacg ccatgcgcta cctcctcacg ggcgacgagt tcgacgtcca ggatgcctac
     6241 cgttttggtc tggttcaaga gatcgtcgcc ccgggacagc aatttgccaa ggcattggag
     6301 attgcggaaa agatcgcctc ccaagcgccc ctggcggtcc aggacacgct cgctttggcc
     6361 aagacggcaa cctacgaggg atttgcggcg ggcgtccggg agatcatgcc gaagctgctg
     6421 aagatcatga agacggaaga tgcggcggaa ggggtcatgt ccttgatcga gcgccgtccg
     6481 gccaatttca aaggccgata gagactgagg cgtcagtcgg gcaggacttc gatcccctgg
     6541 gcggtcatcc aggcggaaag atcggcgagg gagcggcggg cgtattcacg cccccgctct
     6601 ctttttttta tccttagaga cagggggcgc aggaggccgt ggtttatgtt catcggctga
     6661 aaatcatttt ttcccgccgt ggtgatatgt cggatcagac tccccagagc cgtcgtatcc
     6721 ggaggtggtg acagttcacc ccccgtcaac atcctcccga ggctgatgcc ggcgtaaaga
     6781 cccattgccg ctgattccat atatccctcc acccccgtca gctgcccggc gaagaatagg
     6841 tgggggtgtg agatgagctg caggcggtcg ttgatcaagc ccggagaacg gaggaaggtg
     6901 tttcggtgga tgctaccgta ccgggcaaat tccgcctgga cgaggccggg gatcatccta
     6961 aagatccgct cttgttcagg ccgggtgagt ttggtctgga atcccaccat gttgagcagg
     7021 gtgccgcttg cgttttcccg ccgtaactgc acaactgccc agggttgtcg gccggtccgg
     7081 ggatccgtga gccccacggg tttcatggga ccgaagagca gggtccggga accccgctgc
     7141 gccaggatct ccacggggag gcagccttcg aaataacgag ggttttcgaa gctgcgtagg
     7201 ggaaatttat cggcggtgag gatctcctct cggaaggcac gataggtctc ctcatccagg
     7261 ggacagttca ggtagtcgcc ggcgttttcc gcgcttgaag gatcatagcg ggaggcccag
     7321 aaactacggt tcggatctac ggaatcggcg gtgacgatcg gggcgatggc gtcgtaaaaa
     7381 tacaggcatt cccgacccgt aaggcgttga atcgccgcgg ccagggcatc ggaggtgaga
     7441 ggaccggtgg cgacaatggc gggggtggcg gtgggaagct cggtaagctc ctggcggacg
     7501 agctgaaatc gttcccccca ggccaagaga cgttcctgga tgtaggcgga aaaacgctcc
     7561 cggtccacgg cgagagactt cccggccggt agggacgtct ggtcggcggc ggccatgaac
     7621 agcgaaccca ggcgccgcat ctcctccttg agaagcccgg cggcgctggc cggatctccc
     7681 gagcgcaggg aattgctgca aaccagctcg gccagatcag gagaggagtg ggccggagaa
     7741 tatcgctccg gcttcatttc ccagagggtt acgcggcagc cacggcagag caactgccaa
     7801 gccgcttcgc atcccgccag gccgccgccg attatcaatg cctgtcctct atggggcatg
     7861 gacgaccaga tcactcctgt atttgcattc cttacggtgg cattgtttat aacttcccgt
     7921 cttggtgcgc ttttccacca gaaagggggc gttgcacaac ggacaggcct cggggagggg
     7981 ccggctccag gtaacaaatt tgcattcggg atagcaggtg cagccgtaga atatttttcc
     8041 cgtttttgat cgtcgttccg aaaggaatcc gttacagccc ggctccgggc aggggatgtc
     8101 caagtggagg ggatggatga ctttgcaggc gggatacccc gtacagccga gaaatttacc
     8161 ataccggcca gattttatgg ccagcggttt tccgcattgt tcgcagatct tgtcggtgag
     8221 gacctcctct ttcggcggag tgggttcccc gcccgccgcg gtgattttta ccgtcgtctt
     8281 gcattcggga tagccggagc aggccatgaa cttaccgaat cttccctgtt tcagcaccat
     8341 ggtccgaccg catttttcgc aggtaagttc cgggacgcct tcgccatttt tcccctccgt
     8401 accgtttcca gcctcgggga tattcatggt gtttttgcaa tccgggtagt ttgtgcaggc
     8461 gatgaaacgg ccgttcctcc cccatttgat tgccatgggg cttccgcatt tttcgcaaac
     8521 catgtccgtg gggatgccct tgcccttgag actcttcatc tttcccgccg ccacctgcag
     8581 ttcccgggca aattcggcgt aaaaatcctt cagtgtttcg atgcgttcgt ttttgccctc
     8641 ctcgatgcga tcaagccggt cttccaagga ggcggtaaaa gatacatcga ggatcttcgg
     8701 gaagttttcc accagtagtt cggtaaccag catccccaga tcggtgggat gaaatctacc
     8761 tttttccagg cggacatact cccggtcctg gatggtggcg aggatcgcgg cgtaagtgct
     8821 gggcctgccg atcccctttt cttccaattc ccggacgagg gtggcttcgg aaaaccgggg
     8881 cgggggctgg gtgaatttct gttccggggt gagggctgta agtttcagga cttcgccgat
     8941 ggccacttcg ggaagaagcc ggcccatttc atcctctcct tcgccgccgt tatcgtcttt
     9001 gccttcacga taaacttcgg aaaaaccgga aaatttcaag atggagccca gggcgcggag
     9061 ttcatagacc cccgccgcga tgtcgataac cgtctgatcg taacgggccg gggacatctg
     9121 gctggcgaca aagcggttcc agatgaggag atagagcttg tactgatccg gggtaaggtg
     9181 actcttgatt tcgccgggcc ggtattgcaa cgaagaaggg cggatggtct cgtgggcgtc
     9241 ctgggccgag gcgctgttct tgaagtgcct tggttttggc ggcaggaagt caggatcata
     9301 ggaagcccgg atgtaatttc taacctcctg tagggcctcc tcggatatgc gcaccgaatc
     9361 ggtgcgcata taggtgatga ggcctaccga accttccgga ccgagttcaa ccccttcata
     9421 gagctgctgg gctacgttca tggtcttctt tgcggagaag cggagccacc gggaagcctc
     9481 ctgctggagc ttactggtcg tgaacggggg caggggattg cgcttgacct ctttcttctc
     9541 ctctccggtg accacaaaat ccagttcccc cagctcccgc aacacccggt ttgcctcttc
     9601 gctatcgccg atcctggcct tcttgccggc gatcttgacg agcctggcgt cgaacggcgg
     9661 cggcatatgg gcggccaggg aggctgtgat gttccagtac tcttcggaga caaaggagcc
     9721 gatttccttc tcccgatcac agatcaggcg cactgccacg gattgcaccc gccccgcgct
     9781 gaggccccgt ttgaccttct cccaaagaag cgggctgatc ttgtagccca cgaggcgatc
     9841 aagtatccgt cttgtctgct gggcctcgaa tttcgaccgg tcgagggcga tggggttggc
     9901 gatggcgtcc agaacggtct tcttggtcag atcgttgaag agggcgcggt gaagctgtct
     9961 ttttttactg ccgatttctt cggctacatg ccaggcaatc gcctctcctt cccgatccgg
    10021 gtcgggggcg aggaagattc gctctgcttt ttgcgccgcc ttcttgatct cagcgatgac
    10081 ctttttcttg ctgtcgatta caccataact tggagtaaag tcgcgttcca gatcgattcc
    10141 cagagaactc ttgggcagat ccttgacatg gcccattgtc gcccgtacct caaaatcagg
    10201 gccaagaaat ttcttgatgg tcttcacctt ggttggcgat tccacaataa ctaatgaaga
    10261 aggcatgtat ttactcctga agaatatact tgtttcccgg tgtctgccgg atcagtcccc
    10321 gcagttcgag ctgcagcagg gcgggcagaa cttccgatgc ggcaagaccc gtggcagcga
    10381 tgagatcatc gacggtgcgg ggatgggagg agagatggtc aataacaagt ccttccttgc
    10441 cgctaatcga gggccttcgc cacggtgcgc gacccggggg aaaaacggtg gcggccggtg
    10501 gcagtggctt tacctcctcc ggcgcgtcct ccgaaaaagg ggcctgcggt ttgatttcat
    10561 cgaggatgtc atgaatgttt tcgacgagtt tcgccccctc cttaatgagg cggtttgttc
    10621 cccgggaacc ggccgcgtcg attcccccgg gaacggcgaa cacctcgcgt ccctgttcca
    10681 gagcaatccg ggcggtgatc agggaaccgc tcttttccgt ggcctcgacc accaccaccc
    10741 ccagggacat gccgctgatg atccggttcc gggcgggaaa gttcgtggca ttggggggag
    10801 tccccagggg gaactccgat actacggcgc cgctttgggc aatcttccgg gccagcccct
    10861 cgttttccgg gggatagatc acgtcgattc ccgagcccaa aacggcaatg gttctgccct
    10921 tcaccgacag tgcgccccga tgggcggccg tgtcgatccc ccgcgccatg ccgctgacca
    10981 cggtgacgcc gtgcagagac agctcgcggc tgaggcgttc agtggtgaat ttgccgtagg
    11041 cactggccat ccgcgaaccg accatggcaa tattgatatc gtcgggccga agctctccct
    11101 gcacgtagag gagaaaggga aagtcgtaga tgaagaggag ccgtctcgga aagcgggggt
    11161 cctggcaggt catgatagta gccggcaatt ccgccgcccg gtccaactcc gcggcaactc
    11221 gctcccagag attgaaatca ttgatgtttc ttgctgtttt ggcgccgatg ccaggtacgg
    11281 cagccagcat gttcaatggc gcccggaaga tccgttcggg ggcgccgaaa ctctggagga
    11341 gattccgaac accgatgttg cctaatcctt ccacataccg gagggccagc cagtatttca
    11401 tgttttcagg ttgttgcgtc acattccaat cctgccgctt cggcgttaat gaaggttgaa
    11461 cataccggaa taaactgaag tcgtctcccc ggggtcaatc aggagggcat agccggccgg
    11521 aacattgttg ccgaagaaac cgctgcacga tatatctcat ctccaaacgc tgtcaaggta
    11581 tttcatgaga gtcgataata tggatttttt tcaatcatat aacagctgac ggcaaatatc
    11641 tgttgtcaat gagaagcatt tccgataaag gagcgccgga gggaactctg ggggaaggaa
    11701 aatcggcaag atggaaattt ttctctttcc attcccgcgg ctgcccggac cgaaaagtgt
    11761 cgggagactc cggaaaccgg agcggatggg gcaccggaaa tccttgagga ggtttgtcat
    11821 tcaacccttt ccgatgataa aaaaaccatg gtggatactt tggtccgtgt tgttggcggt
    11881 ggtagccatt cagcctttgg cgccggtctg gagcgccgag gtcctgctcc atccggaggc
    11941 gatcgacctt ggggacgtag gcgccggcat attcaaaaaa ggccaggcga tcatgacctt
    12001 tgcggatgcg gaacggaaag actgggcgat tgcggcgccg gaaggatgga cctgtaaagc
    12061 cgggcaaccc ctgacaggga ctatcgcaca ccgccatccc gccaagatca atattgccat
    12121 gaagtcttct ctggcgccgg tgggtgaaaa cggtagacat caacatcttt ataatgtgca
    12181 attgcacctg gaattcgaaa acatgcaggt cgtctatcag cggctcattg ccgccggacc
    12241 ccatcgagaa ggaatagcct tcaatattgc cggagagctg aaggtcatcg aactatcatt
    12301 tcatatccgg gaatatcccc cggagccggt gcttgccatc cgtccccagc ggctcgattt
    12361 cggtcttcta gaaccaggcc ggcaggtgga gggtaagatc gaaatcacca accgggggcg
    12421 tcagccactg aaatggaaaa ttgaaccaac ccgctggccc gccgacgggg agaacggtga
    12481 agccgccgcg gggcaatatc actccttcca ccgtccggag gtaacgggaa caggcgccta
    12541 tcgcccggat ccgactcgga gcggagcggc ggagttcaaa ggaaactgga cggaaaacga
    12601 aggctatccc gaggcgcagg acaaggcggt attgaagtac aattattccg gaacggggat
    12661 catcgtccat ttctggaaat caccgctggc ccggtccatg aaggtgtatc tggacaaccg
    12721 tcccctcggc aatctggagg cattcggtga gcagatcgcc gctgccgaat acctcctgcc
    12781 agaaacaccg gcgcctggtt cccataccct gaccctggtc aaccagcagg ggagaactgt
    12841 cgtggagggg gtaaagatcc tggggaggga agtggtgaaa gaccaaggga aatgggtccg
    12901 ggtctttccg gatgtggggg tcacgacccg tgaggtcgat tacctgcata tcgctgtgga
    12961 tacggcaaat ctgaaaccgg gggcccatgg ccgctgtttc cgaatagatt ccaatggcgg
    13021 cagcgcggta gtggagatcc atgcggaagt ggcgtcggaa gcgggggcga agattttgga
    13081 cgtttatcgt taccgcaacg gggacgactt tctcttcacg accaatcccc agggagaggc
    13141 cgggatgctc tcagcgcgta attaccggaa agacggcatc gccttccgcc tcttcagccc
    13201 gggaacaccc gggacgacgg aattttatcg ctggtacaat ccccgtcagc aatgtcactt
    13261 ttattcctat gaggccggcg ggggggagaa gttgtcccga gactacatca tggaagggac
    13321 catcggcaac attgccacca gccgcctgcc caacacccgt cctttgcatc gctggcgtca
    13381 tatcggcagg gaacagtttt ttttcaccac ggactcccgg agcgagggct acaacagaaa
    13441 aggctaccga tacgacggaa tcgccggcta tgtgcgttga tcgttaattt tcgcgaagga
    13501 tatgattcat gcttgacaaa agccgtcaaa aaagatatta ggctccctct tttacatagc
    13561 atgcgcctgt agctcagctg gatagagcaa cggacttcta atccgtgggt cgagagttcg
    13621 aatccctcca ggcgtaccaa gcgaatatgg tgggtgtagc tcagttggtt agagtgccgg
    13681 gttgtggccc cggaggtcga gggttcaaat cccttcactc accccattga ttgcattaaa
    13741 aaggcgcctg tagctcagtt ggatagagtc gcagacttcg aatctgttgg tcgtacgttc
    13801 gagtcgtacc aggcgcgcca gatataaaaa aataggttct cttcagcaat accgggcctt
    13861 tagctcagtt ggtagagcag ttgactctta atcaattggt cgggagttcg agtctcccaa
    13921 ggcccaccag aaaatcaagg ggattcaggt agatagccgg aagccccttt ttttgtttct
    13981 cgtcccgtgg ctaataaaga aatatggacg g
//
LOCUS       DAOSMF010000038        13972 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_5120_length_13972_cov_18.124021, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000038 DAOSMF010000000
VERSION     DAOSMF010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 13972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13972
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5120_length_13972_cov_18.124021"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            252..1064
                     /locus_tag="PLT63_05635"
     CDS             252..1064
                     /locus_tag="PLT63_05635"
                     /inference="COORDINATES: protein motif:HMM:NF022932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOD98237.1"
                     /translation="MEEGFRDLFQATVDALNNMGLTVYEAKTYLTLLQKHPSHGHEIS
                     RRSGVPGPKIYETLSRMVQKGLVAVLNTDPQMYSPLPYREFLTRKSNEFQVTEGLLAA
                     NLEKISMPAVDITLWQLTNHDVLIAKARDLIDGAKKAVLTSMWPPQGRLLEKSLAAAR
                     QRGVAIVSIQFEQEVIEVGKVFRHVQVDTVYDRHSGELTMVVDESVGLFMSRPPDREW
                     NGFWTTNPGVVKLMTNYIRHDIYSNKLIYRFEKAVKEEFGEQLQELLNLEVD"
     gene            1149..2072
                     /locus_tag="PLT63_05640"
     CDS             1149..2072
                     /locus_tag="PLT63_05640"
                     /inference="COORDINATES: protein motif:HMM:NF017652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HOD98238.1"
                     /translation="MREKRIITAALTGAIHTPSMSPYLPVTPEQLIDEAVRAYEAGAA
                     VAHVHVRNPETGLPCADQEIFRTFAEGVKKLCDMVICCTTGGRLGEPVENRVRVVTSL
                     SPELATLNAGSLNFALFHIADNYEQWTHDWEKGYLAATEDFIFPNTFLTMRQFLEKFA
                     AQGTKPEFEIYDVGMINNLAFLIEKGHVKKPVYLQFVMGILGGIPATIENFVILLETA
                     RKAIGDFQFSVCAAGKTQFNLCTTSLMMGGHARVGLEDNLFLEKGQLAKSNAEQVAKI
                     VRIARELGVEAATPAEARQILGLKGIDKVNF"
     gene            2088..2867
                     /locus_tag="PLT63_05645"
     CDS             2088..2867
                     /locus_tag="PLT63_05645"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HOD98239.1"
                     /translation="MGKRFIDLSIAIEASLPCDPPMMIPRIDYVDHTAGATQMLDFFP
                     GIKREQLPGGLGWALEFINLTTHSGTHLDAPYHYHPTQDQGKRALTIDEVPLDWCMND
                     GVLLDFRHKGDGERITAADVQKELDRIGYELKPLDIVLVQTGADAAWGTAEYLVKGPG
                     MTRESTLFLTERGIKVVGIDAWSWDRPLPFLAREFKDKGDPKVIWEAHFAGIEMGYCH
                     MEKLSNLAAIGRPHGFTVCCFPVKIKGASAGWCRPVAIVNE"
     gene            2877..3797
                     /locus_tag="PLT63_05650"
     CDS             2877..3797
                     /locus_tag="PLT63_05650"
                     /inference="COORDINATES: protein motif:HMM:NF017652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HOD98240.1"
                     /translation="MSKAIFTAAITGGIHTPSMTPYLPITPKQIADDTVRAWEAGAAV
                     AHIHARNPETGQPSSSLDLYREAITDIKSRCDIILCITTGGGLGMSTEQRLAVIPAFA
                     PEMGSFNFGSINVALYPLLAKHSNFKFPWEPAFLAMTEDYIFPNTFKSMREFAATFKE
                     HGTKPEIEIYDVAMINNLAHMIEGGHIEKPVYLQFVMGLLGGIPARMDNLLFLYKTAK
                     EAIGDFVWSVCAAGQYQMKMGTAALLLGGNVRVGLEDSMYLEKGQLAKSNAEQVANIV
                     RIAKALGIEPASPAEARGILGLKGLDKVKY"
     gene            3880..4650
                     /locus_tag="PLT63_05655"
     CDS             3880..4650
                     /locus_tag="PLT63_05655"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HOD98241.1"
                     /translation="MNGKITIIDLSVPTEESPSEALSLKVTHESHRETAPVLAGFFGC
                     KESDLRNGLGYANDRVEMISHSGTHLDAPWHYAPQSEGKAARTIDQIPLEWCYGDGVV
                     LDFRHKPKGAAIGSADLQRELGRIGYSLQPYDIVLIMTGADKFWGQAEYFDAGCGMSR
                     ESTLWLVEQGIRVMGIDAWGWDRPFWAIKEDFAKTGDRNILWEAHLAGIEREYCHLEK
                     LANLDKLPRPFGFKVACFPVKLAGGSAGWCRAVALVEK"
     gene            4808..5539
                     /locus_tag="PLT63_05660"
     CDS             4808..5539
                     /locus_tag="PLT63_05660"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD98242.1"
                     /translation="MLTGKKITKRFGGLTALSEVDFEVRRGEIVGLIGPNGSGKTTLF
                     NVISGIYRPEEGDLFFEGRRITDFPPYKRAGLGIGRTFQIVRPLTGLNLIDNVVTGVL
                     YGRKHTAATKGAREKAKEILDFTGLTQKARRLPGELVLEDRKRLEIARALALNPEILL
                     LDEVFAGLNPTEISSAIELTFRIRDTYGTTIFMIEHVMKAIMSTCSRIMVLNFGIKIT
                     EGPPEEVANHPEVISAYLGAAYAAC"
     gene            5526..6230
                     /locus_tag="PLT63_05665"
     CDS             5526..6230
                     /locus_tag="PLT63_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD98243.1"
                     /translation="MLRVDNITVCYGRMEALRGVSLEVAPGEMVALIGGNGAGKTTLL
                     NAVSGLVPAAGGGISWEGKPLKGFAPDRICREGIVQVPEGRKLFPAMTVEENLIMGAY
                     LPTVRKASRKTLAHVYEIFPRLADRRRQLAGSLSGGEQQMAALGRALMARPRLLMLDE
                     PSLGLSPKAAVEIFATVRQLNKEGLSVLLVSQEVLETLAITARGYVLENGVVAMEGPS
                     AELRENPQVKTSYLGL"
     gene            6296..7504
                     /locus_tag="PLT63_05670"
     CDS             6296..7504
                     /locus_tag="PLT63_05670"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HOD98244.1"
                     /translation="MKGKRMKGFSLFMTVTVLCGLLLTVPAAAIGAGKPIVIGGSLPL
                     TGEFAETGLWIERGMRYWAEEVNAKGGLLGRHVEFKIYDDQSNVGKAVTFAEKAITVD
                     KVDLLFGGYPGTAARAVMAVAEKHKMVYVSMGGHMKSFQQGYTYSFGAPPLMGEWWYE
                     GFFQWLNTLPANQRPKRAAVYTINNPVGLSLQDSINRWTKYLKIDVVINEKYNSPLTD
                     ATSLIVKAKQMNCDILFSNGFFPDGVTTIKAAKALDYNPKAIVQGIGSVVPAWVKELG
                     RDGDYVFSGTVLHGMLNYQGNDKLNAYVKKKYKLPAYPLYFGFGYAWMQTLQQGVTGA
                     KSLDQTKIRDWLKTHQVNTIAGPMTFDKYGLPAPINFATQIINGKVELIFPKNVRTKE
                     PVYPKPAWKK"
     gene            7506..8381
                     /locus_tag="PLT63_05675"
     CDS             7506..8381
                     /locus_tag="PLT63_05675"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOD98245.1"
                     /translation="MDAVLVIQSLISGIMIGMVYALLSVGFSLTWGVMHVIHISHAAF
                     GLLGAYLAYSLLAYFGIDPVVSAAVSVPLLFILACGVYQLLIKPITRAKEVIVASMIL
                     TFGLAIIMENVMVLIWKADPRIMTPTYASGALIVGPFYFQYPHLVGFTLSIAGIAAIY
                     FFLKKTYTGMAVRAAWQQPEAAQLYGVNLYRISMITFALAVSTGGAGGISLALLNSFE
                     PHSHNLWLIYLFLVVIVGGVGNVAGTALAGLIIGVITGLSMAFLPFQWVNLVTFGFLM
                     VFLILRPHGLFKQGV"
     gene            8384..9322
                     /locus_tag="PLT63_05680"
     CDS             8384..9322
                     /locus_tag="PLT63_05680"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOD98246.1"
                     /translation="MKKAPLAATAIIIVMLAAFPFLSPEVYYLSFMFSVFMYVILASS
                     WNLIGGYAGYLSFGHAAFFGIGAFATAKMAAVFELPPVLTILAAIPAGAIAALVALII
                     GYPCLKIRGPYFAVVTLCFAFVVDLAVKNIEYLGGAEGIWMQIMDVPEKLSRSIMYEV
                     MLLLMVVTLAVAWWIGRSRLGAGLRAVRSDEEVAETMAIPTARIKIQAFILSAFFPGI
                     AGGLFAYYLTYIHADVVFNINISILIVLMAMFGGGGTWLGPIIGAVILTVVNELLSTF
                     AQAEIARIIYGALFIVVTIFMPNGILDFFRKKNRSR"
     gene            9456..12089
                     /locus_tag="PLT63_05685"
     CDS             9456..12089
                     /locus_tag="PLT63_05685"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOD98247.1"
                     /translation="MNIKEIFRNLSMEGKGLYYKLTIVFALFFLVPLTGFFFFVFRYQ
                     LLHDEYIPIFIFAVLVFSLFAFSIVRNIFDNISSLAKRVTGAVAMEGQGAQAAGAGKQ
                     NATDEIQNIISSFQSLETQLNRQFVSLNQKVSQISTLKELADLCYVTLESDDLLYITL
                     ERAMKLVNGDIGSVLILERGKRDYFIVHAAIGHGDKLQSGDRIDFAKSIAKFAVINKA
                     PLLVEDIEKDDRFGRRSKDEYGTKSFLCMPLKGIKDVFGVLTVSRRREDLPFTAADAD
                     VLAPLVSNAAFTYDNLSLIKEKSYKDEVLSTVDHIAKIFGSTVAKGELIPSLLAQIGI
                     TIPFDIAVILAKESESSSRLSVLEYRTKMDVPFHKGDAYPYEGSLIDKVLQDGNAIIL
                     PDLDTAPKLTGLEILVAAAAKAAILAPLKMGGQVHGVLLLGSRAPAGITEAEEKIDML
                     VSLVSLAIVLERLEASISQREREMDFVKQIGSILASSTFDLNEVIKQTMNMIRALMDV
                     EGGSLLLRQEDHLVFREAFNIHEHVNMELLKNLRLKLGQGIAGYAAARGESLIVANTE
                     GSRYFYPEFDRNSGFQTRSVLAVPLISQGRILGVIEVLNKVSGEFTDSDMKLLQSIAT
                     SVSIALENARLYQETKSLAEHERGIRNVFQKFVPKEVVERIIHGSETGEGVMEEIRLL
                     TILNIDLRGFSILSSQFSPNRTVTILNHFFGVIGEIILKHRGIVDKYLGDGLLGIFGA
                     PASSGYDADNAVAAALEMQAAMKGINHHVHTLVDQPLTMGISIHTGEALIGNIGFDKK
                     MDYTVIGDSVNVVFRLQSLTKYKPDSILITEMTHQAVVKSVLQVAPLEVQDEDIAKQI
                     NFMRVYELLGQQSRHGYPENA"
     gene            complement(12173..13681)
                     /locus_tag="PLT63_05690"
     CDS             complement(12173..13681)
                     /locus_tag="PLT63_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOD98248.1"
                     /translation="MTAGGKTIRVGVMAGRRELRGCFHGSFRTPAGEILTGPFVLTSG
                     PPSPHADGDSPPSSGAGDEGTGLWLQTQTCRLFLPPEECRFHSGERADFSLDAVTIGV
                     NFHWERRERQTFQGSLVALAEPDGLLTAINEIDIEDYLASVISSEMAATAPVEFLKAH
                     AVMSRSWLLAMLERKNLENAGAGCGRTRSVGCVVRNSAGGETGGMDGDVAAARGGESK
                     RVNSDHDGDIPEEIVKWYGREEHTRFDVCADDHCQRYQGVTKIVSPRVREAIDTTRGM
                     VLVYGGRICDARYHKACGGRTEDFANVWEERSVPYLTSIADGPEDFPSLATEAAAADW
                     ISSSPAVYCNTGDMTALRRILPAFDQETPDFFRWEVAYERRELEELLKEKSGIDFGEL
                     LRLNPLSRGPSGRIIRLEIAGTRHTVIVGKELEIRRWLSPSHLYSSAFVVAAEKDTRG
                     KIRRFVLRGAGWGHGVGLCQIGAAMMAIRGFSMETILGHYFPGAVLRINSDR"
BASE COUNT         3022 a         4226 c         3906 g         2818 t
ORIGIN      
        1 gaaggcggtc ttgatactcc ttttcgaacc gaatgacttc ctcatgcgca actccctgac
       61 gcccaggcgt caaattactt acaaaggaaa aaagagcata taaagtaata aattcaatat
      121 aacttcattt tcatgcattg tggcgctcca cggtgcatgc gggttttttc acgactcaac
      181 aggtttttaa cttgacaacc agtctttgca tctttaaact acctccgtag ttatgtcaag
      241 acggggtgga catggaagaa ggattccgcg acttattcca ggctacggtg gatgccctga
      301 acaatatggg cttgacggtc tatgaagcca agacctatct aaccctcctg cagaaacacc
      361 cctcccacgg ccatgagata agccgccgtt ccggagtccc cggtcccaag atttacgaaa
      421 ccctgagccg gatggtgcag aagggactgg tggccgtgct gaataccgat ccccagatgt
      481 attcccccct gccctaccgg gaattcctca cccgcaaaag caacgaattc caggtaaccg
      541 aggggctcct ggcggccaat ctggaaaaga tctccatgcc ggccgtcgat atcaccctct
      601 ggcagttgac caatcatgac gtcctcatcg ccaaggcccg ggatttgatc gacggcgcga
      661 aaaaggccgt cctgacaagc atgtggcctc ctcagggcag gctgttggaa aaaagcctcg
      721 ccgccgcccg tcagcggggg gtggcgattg tttccatcca gttcgaacag gaagtcattg
      781 aggttggcaa ggtttttcgc catgtccagg tggatacggt gtatgatcga cacagcgggg
      841 aattgaccat ggtggtcgat gagtccgtgg gcctcttcat gagccggccc ccggaccggg
      901 agtggaacgg cttctggacg accaatcccg gggtggtaaa actcatgacc aactacatcc
      961 gtcatgacat ctattcaaac aagctgattt accgtttcga gaaagcggtc aaggaagaat
     1021 tcggagagca gttgcaggaa ttgctgaacc tggaggtgga ctgagcatcg cttaggatct
     1081 gtctatcagc cgttggaaat cggcgccgtc ccattgcaga ccttcagtga gaaaggagct
     1141 ttgccgttat gagggaaaaa agaatcatca ccgccgccct gaccggggcg atccacaccc
     1201 ccagcatgtc cccctatctg cccgtcaccc cggaacaact catcgacgaa gcggtacggg
     1261 cttatgaggc cggggcggcc gtcgcccacg tccatgtccg caatcccgaa accggccttc
     1321 cctgcgccga ccaggaaatc ttccggacct ttgcggaagg ggtaaagaaa ctttgcgata
     1381 tggtcatctg ctgcacaacg ggcggtcgcc tgggcgaacc ggtggaaaac cgcgtccgcg
     1441 tcgtcaccag cctgtcgccc gaactggcta ccctcaacgc cggctccctc aatttcgccc
     1501 tcttccatat cgccgacaac tacgagcagt ggacccacga ctgggaaaag gggtatttgg
     1561 ccgccacgga ggacttcatc tttcccaaca ccttcctgac gatgcggcag tttctggaaa
     1621 aattcgccgc tcagggaacg aaaccggaat tcgagatcta cgacgtcggg atgatcaaca
     1681 acctcgcctt tctcatcgag aagggccatg tgaagaaacc cgtttatctc cagtttgtca
     1741 tggggatcct cggcggcatc ccggcaacga tcgaaaactt cgttatcctc ctggagaccg
     1801 cccggaaggc catcggtgat tttcagtttt ccgtctgcgc cgccggcaag acccagttca
     1861 atctgtgtac gacctccctg atgatggggg gacacgcccg ggtcggcctg gaagacaatc
     1921 ttttcctgga aaaggggcag ttggcaaaaa gcaacgccga gcaggtagcc aagatcgtcc
     1981 gcatcgcccg ggagctcggc gtcgaggcgg cgacaccggc ggaggcgcgg cagatcctgg
     2041 ggctcaaggg aattgacaag gtaaacttct aacgagagga ggaaaaaatg ggcaaacgat
     2101 tcatagatct gagtatcgcc atcgaggcgt ctctcccttg cgatcccccc atgatgatcc
     2161 ccaggatcga ttatgtcgat cacacggcgg gggcaacgca gatgctcgac ttcttcccgg
     2221 gaatcaagcg ggagcagctc cccggcggcc tcggctgggc gctggaattt atcaacctca
     2281 ccacccacag cggcacccac ttagacgccc cctaccacta ccaccccacg caggatcagg
     2341 ggaagcgggc ccttaccatt gacgaggtcc ctctcgactg gtgtatgaat gacggcgttc
     2401 ttctcgattt ccgccataag ggcgacgggg agcggattac cgccgccgac gtgcagaagg
     2461 agttggacag gatcggctac gagttgaagc ccctggatat cgtcctcgtt cagacgggcg
     2521 ccgacgccgc ctggggtacg gcggagtatc tcgtcaaggg accgggcatg accagggaaa
     2581 gcaccctgtt cctgacggag agaggcataa aggtagtagg catcgacgcc tggagttggg
     2641 accgtcccct gcctttcctg gcccgtgaat tcaaggacaa aggggacccg aaggtcatct
     2701 gggaggccca ctttgccggc atcgagatgg gatattgtca tatggagaag ctgtccaatc
     2761 ttgccgctat cggcagaccc cacggcttta cggtctgttg cttccccgtc aagatcaaag
     2821 gggcctccgc cggctggtgc cgccccgtag ccattgtcaa cgaataagga gagcttatgt
     2881 caaaagcgat ttttacggcg gcaattaccg ggggcatcca taccccctcc atgacccctt
     2941 acctgcccat cacccccaag cagatcgccg acgatacggt ccgggcctgg gaggccgggg
     3001 ccgccgtggc ccatatccac gcacggaacc ccgaaacggg gcaaccttca tcatccctcg
     3061 atctctaccg cgaggcgatt acggacatca agtcccgttg cgacatcatc ctctgcataa
     3121 cgacgggcgg ggggctggga atgagcacgg aacagcgcct ggccgtcatc cccgccttcg
     3181 cgccggagat gggatcgttc aatttcggct ccatcaatgt ggccctttac ccgctcctcg
     3241 ccaagcacag caatttcaag ttcccctggg agccggcgtt tctggccatg acggaggact
     3301 acatcttccc caacaccttc aaaagcatgc gggagttcgc cgccaccttc aaggagcacg
     3361 gcacgaagcc ggagatcgag atctacgatg tggccatgat caataacctg gcccacatga
     3421 tcgagggggg gcacatcgaa aaacccgttt acctgcagtt cgtcatggga ctcctgggag
     3481 gaatccccgc caggatggac aatctgctct tcctctacaa aacggcgaag gaagccatcg
     3541 gagattttgt ctggtcggtc tgcgccgccg gtcaatacca gatgaagatg ggcacggccg
     3601 ccctgctgtt gggcggaaat gttcgcgtcg gtctggaaga cagcatgtat ctggaaaaag
     3661 gacagttggc caagagcaat gccgagcagg tggcaaacat cgtgcggatt gccaaagcgc
     3721 tgggcatcga accggccagc cccgcggagg ccagaggcat cctcggcctg aagggactgg
     3781 acaaggtaaa atattgacga tttcgcaaac cgtcccatca tggagaggtt accatgtttt
     3841 tagtgacgat caagacttcg aggaaagcga ggtgaagggg tgaacggaaa gattaccatt
     3901 atcgacctga gcgtaccaac cgaggaaagc ccgagcgagg ccctttccct gaaggtgacc
     3961 catgaaagcc atcgtgagac ggccccggtg ctggcgggtt ttttcgggtg caaggaatcg
     4021 gatctccgca acggcctggg ttatgcaaac gaccgcgtgg aaatgatatc ccactcgggc
     4081 acccatctgg acgccccctg gcactacgcc ccccaaagcg agggaaaggc ggcccgcacc
     4141 atcgatcaga ttcccctgga gtggtgctat ggcgacggcg tcgtcctcga tttccgtcac
     4201 aaaccgaaag gagcggcgat cggcagtgcg gacctgcaaa gagagctggg ccgcatcgga
     4261 tactccctgc agccctatga catcgtcctg atcatgacgg gggccgacaa gttctggggg
     4321 caggcggagt atttcgacgc cgggtgcggc atgagccggg aatcgaccct ttggctggtg
     4381 gagcagggca tccgggtcat gggcatcgat gcctgggggt gggaccggcc gttctgggcc
     4441 atcaaggaag atttcgcaaa aacgggcgac cggaacatcc tctgggaagc ccatctggcg
     4501 ggcatcgaac gggaatactg ccatctggag aagctggcca acctggacaa gctgcccagg
     4561 ccattcggtt tcaaggtggc ctgctttccc gtaaaactcg ccgggggcag cgcgggctgg
     4621 tgccgtgccg tagctttggt ggaaaagtga ggacgggaca gattattgag gtggaaacga
     4681 cgggaagctt tacgcttatc aatcaccctc cccttctatg gggagggacg gagaaggcgg
     4741 aaaatacacg cgcctaatca tatctccctc cccttcaagg ggaggatggg gtggggatgg
     4801 ggttaaaatg ctcacaggta aaaagataac caagcgtttc ggcggtctga cggccctttc
     4861 ggaagtagat ttcgaggtgc gccgcggcga gatcgtcggc ctcatcggtc cgaacggatc
     4921 ggggaaaacg actcttttca acgttatctc cggcatttac agacctgaag agggagacct
     4981 gtttttcgag ggacggagaa taacggattt ccctccctat aaacgggccg gtctgggcat
     5041 tggccgcacc tttcagatcg ttcgccccct gacgggcctg aacctcattg ataatgtcgt
     5101 caccggcgtt ctttacggcc gcaagcatac ggccgcaacc aaaggcgccc gggagaaggc
     5161 caaggagatc cttgatttta cgggcttgac gcaaaaggcc cgccgccttc ccggtgaact
     5221 cgtcctggaa gaccgcaagc gtctggaaat agcccgggcg ctggccctca atccggaaat
     5281 cctgctcctg gacgaggtct ttgcggggct taatccgacg gaaatcagca gcgccatcga
     5341 gttgaccttc cggatccggg atacttacgg aacgacgatc ttcatgatcg aacacgtgat
     5401 gaaggccatc atgtccacgt gcagccgcat catggtcctg aatttcggga tcaagatcac
     5461 cgaaggaccg ccggaagagg tggccaatca tccggaggtc atctccgcct atctgggagc
     5521 cgcttatgct gcgtgttgat aatataaccg tctgttacgg ccggatggag gccctgcgcg
     5581 gcgtgtccct ggaggtcgcc ccgggggaaa tggtggccct catcggcggc aacggcgccg
     5641 gtaagacaac cctcctcaac gccgtctccg gcctggtccc cgcggccggc ggcggtataa
     5701 gctgggaggg caaacccctg aagggttttg ccccggaccg gatatgccgg gagggaatcg
     5761 tgcaagtccc ggagggacgc aagctctttc ccgccatgac ggtggaagaa aacctcatca
     5821 tgggggcata tttgccgacg gtccggaagg cgagccggaa aaccctcgct catgtatacg
     5881 aaatcttccc ccgccttgcc gaccggcgac gtcaattggc cggcagcctc tcgggaggag
     5941 aacaacagat ggcagccctg gggcgggctt tgatggccag gccccgtctc ctcatgctgg
     6001 atgaaccttc cctgggtctg tcacccaagg cggcggtaga aatctttgcc accgtcaggc
     6061 aattgaacaa ggaagggctg tcggtgctcc tggtatccca ggaggtcctg gagacactgg
     6121 cgatcaccgc ccgcggatat gtcctggaaa acggagtggt ggccatggaa ggcccttccg
     6181 cggaactccg ggagaatccg caagtcaaga cgtcctattt aggactataa gggtttatca
     6241 cgaaataacc gtggcagatc ctaagataaa ccaagcattg gaaggaggtt tcataatgaa
     6301 agggaaacgg atgaaaggct tctctttatt catgaccgtc accgtacttt gcggcctgct
     6361 gttgacggtc ccggcagcgg ccatcggcgc cggcaaaccc atcgtcatcg gcggctcgct
     6421 gcccctcacg ggtgaatttg ccgaaacggg cttgtggatc gaaaggggca tgcgctactg
     6481 ggcggaagaa gtcaacgcca aaggggggct cctgggacgg catgtggaat tcaagatcta
     6541 cgacgaccag tccaatgtgg gcaaggccgt aaccttcgcg gaaaaggcca tcaccgtaga
     6601 taaggtcgat ctcctcttcg gcggctatcc cggcaccgcc gcccgggccg tcatggccgt
     6661 ggcggagaaa cacaagatgg tctatgtctc catgggcggc catatgaaga gcttccagca
     6721 gggctatacc tactctttcg gcgccccgcc gctgatgggc gagtggtggt acgaggggtt
     6781 cttccagtgg ctgaacaccc tgcctgccaa ccagcgtccg aagcgggccg ccgtctatac
     6841 gatcaacaat cccgtcggtc tatcgctgca ggactccatc aaccgctgga caaaatacct
     6901 caagatcgat gtcgtgatca atgaaaaata caactccccc ctgactgacg ccacctcgct
     6961 catcgtcaag gccaagcaga tgaactgcga catcctcttc tccaacggct tcttcccgga
     7021 cggcgtcacg acgatcaagg ccgccaaggc cctcgactac aatcccaagg ccatcgtcca
     7081 ggggatcggt tccgtcgtcc ccgcctgggt aaaggaactg ggaagggacg gcgactatgt
     7141 cttttccggg accgtcctcc acggcatgct caactaccag gggaacgaca aactcaacgc
     7201 ctatgtgaag aaaaaataca aactgccggc gtaccccctc tatttcgggt tcggctacgc
     7261 ctggatgcag accctccagc agggagtgac aggcgccaag agcctggatc agacaaagat
     7321 ccgcgattgg ctgaagactc accaggtcaa taccatcgcc ggtcccatga ccttcgacaa
     7381 gtacggcctc ccggcgccga tcaacttcgc cacccagatt atcaacggca aggtggagtt
     7441 gatcttcccg aagaacgtcc ggacgaaaga accggtctat ccgaaaccgg cctggaagaa
     7501 gtgaagtgga cgctgtctta gtaatacagt cgctgatcag cggcataatg atcggaatgg
     7561 tgtatgcact cctcagcgtc gggttctccc tgacctgggg agtcatgcac gtcatccaca
     7621 tctcccatgc cgccttcgga ctcctggggg cctatctggc ttacagcctg ctggcgtatt
     7681 tcgggatcga tcccgtcgtt tcagcggcgg tgtccgtacc gctgctgttc atcctggcct
     7741 gcggggtcta tcagctcctc atcaaaccca tcaccagagc caaggaggtc atcgtggcgt
     7801 cgatgatcct caccttcggc ctcgccatca tcatggaaaa cgtaatggta cttatctgga
     7861 aggccgatcc ccgcatcatg acgccgactt acgccagcgg cgccctgatc gtgggcccct
     7921 tctattttca gtatcctcat ctcgtcggtt tcaccttgtc tattgcagga attgccgcca
     7981 tctatttctt tttgaagaaa acctacacgg ggatggccgt ccgggccgcc tggcagcagc
     8041 ccgaagccgc ccagttgtat ggcgttaacc tttaccggat cagcatgatc accttcgccc
     8101 tggccgtctc tacggggggc gccgggggca taagcctggc gctcctcaat tccttcgagc
     8161 cccactccca taatctctgg ctgatctatc tctttttagt ggtcattgtg ggcggagtgg
     8221 gcaatgtcgc cggcacggcc ctcgcgggac ttatcatcgg ggtgatcacg ggtttgtcca
     8281 tggcctttct tcccttccag tgggtgaatc tcgtcacctt cgggttcctc atggtgtttc
     8341 taatcttacg gccccacggc ctgttcaagc agggggtata gaaatgaaaa aagcccccct
     8401 ggccgctacg gcaatcatca tcgtcatgct cgccgctttc ccctttctct caccggaggt
     8461 ctattacctg tccttcatgt tttccgtttt catgtatgtg attctcgcct cctcgtggaa
     8521 tctcatcggc ggctacgccg gctacctctc cttcggccat gcggccttct tcggcatcgg
     8581 cgcctttgcc acggcaaaga tggcggctgt tttcgaattg ccgcccgtgt tgaccatcct
     8641 ggccgccatc ccggcggggg cgatagcggc cctcgtcgcc ctcatcatcg gctatccatg
     8701 cctgaagatc cggggacctt acttcgccgt cgtaacgctc tgcttcgcct ttgtcgtcga
     8761 tttggccgtc aagaatatcg aatacctcgg tggggccgag gggatctgga tgcagatcat
     8821 ggatgtcccg gaaaagctct cccgctccat catgtacgaa gtcatgctcc tcctcatggt
     8881 cgtcactttg gccgttgcct ggtggatcgg ccgctcgcgt ctcggcgccg gccttagagc
     8941 cgtccgctcc gatgaagagg tagccgagac catggccatc cccacggcaa ggatcaagat
     9001 ccaggccttc attctctccg ccttcttccc cgggatcgcc ggcggcctct tcgcatatta
     9061 cctgacctac atccacgccg acgtcgtctt caatatcaat atctccatcc tcatcgtcct
     9121 catggccatg ttcggaggcg ggggcacctg gctgggaccg atcatcgggg ccgtcatcct
     9181 caccgtggtc aacgagttgc tctccacctt cgcccaagcg gaaatcgccc ggatcatcta
     9241 cggcgccctc ttcatcgtcg tcaccatctt catgcccaac ggcatcctgg atttcttccg
     9301 taaaaagaac cgcagtcggt gaaaggaccg gcggggaccg gctcggcctc ggatccagcg
     9361 gtattttttt cattatttca ttgacatatc ggactatttt ctctaaaaaa ctaaggcagg
     9421 gtagaaataa ttgaccaaga aaggcatgcc agcccatgaa cataaaagag attttcagga
     9481 atctctccat ggagggcaag ggcctttact acaagctcac cattgttttt gccctcttct
     9541 tcctcgtgcc gctcaccggc ttctttttct tcgtcttccg ctatcagctc ctccatgatg
     9601 agtatatccc catcttcatc ttcgccgtcc tcgttttttc cctttttgcc ttctccattg
     9661 tccggaacat cttcgacaac atctcctccc tggccaaaag agtcactgga gccgtcgcga
     9721 tggaaggtca gggcgcccaa gccgccggcg ccggtaaaca gaacgccacc gatgaaatcc
     9781 agaatatcat ctcgtccttt caatctctgg aaacgcaact gaaccggcag tttgtctccc
     9841 tgaaccagaa ggtctcgcaa atctccaccc tcaaggagct ggcggatctg tgctacgtga
     9901 ccctcgaatc cgacgatctg ctctacatca ccctggagag ggcgatgaaa cttgtcaacg
     9961 gcgacatcgg ctccgtcctc atcctcgaga gggggaaacg ggactacttc atcgtccatg
    10021 ccgccatcgg ccacggcgac aagctgcaaa gcggcgaccg cattgatttt gccaagagca
    10081 tcgccaaatt cgccgtaatc aacaaggcac cgctcctcgt cgaagacatc gaaaaagacg
    10141 atcgcttcgg cagacgaagc aaggatgagt atggaaccaa gtccttcctc tgcatgccgc
    10201 tgaaggggat aaaggatgtg ttcggggtcc tgacggtctc ccgccgccgg gaagatctgc
    10261 ccttcacggc ggcggatgcg gacgtcctgg cgccgctggt aagcaacgcc gccttcacct
    10321 acgacaatct gtcgctcatt aaggaaaaga gctacaagga cgaggttctt tccacggtgg
    10381 atcacatcgc aaaaatcttc ggctccaccg tggccaaggg ggaactcatt ccttcgctcc
    10441 tggcccagat cggcatcacc atccccttcg acatcgccgt cattctcgcc aaggaaagcg
    10501 aatcctcgtc acggctttcc gtcctggaat accgtacgaa aatggatgtt cccttccaca
    10561 agggcgacgc ctatccttac gagggatcct tgatcgacaa ggtgctgcag gatggtaacg
    10621 ccatcatcct tccggacctc gacaccgccc ctaaactgac ggggctggaa atcctggtgg
    10681 ccgccgcggc caaagccgcc atcctggccc cccttaaaat gggcggacaa gtacacgggg
    10741 tgctgctact ggggagcagg gctccggcgg gcattacgga ggcggaagaa aagatagata
    10801 tgctggtctc ccttgtctct ctggcgattg tcctggaacg cctggaagcg tccataagcc
    10861 agcgggaacg ggaaatggat ttcgtcaagc agatcggcag cattctggca tcttccacct
    10921 tcgatctcaa cgaggtcatc aaacagacga tgaacatgat ccgcgccctc atggacgtgg
    10981 agggcggctc gctgctcctg cggcaggagg atcatctggt cttccgggag gccttcaata
    11041 ttcatgaaca tgtaaacatg gagctcctga agaatctccg gctcaagctc ggacagggga
    11101 tcgccggcta tgccgccgcc cggggggaat cccttatcgt ggccaacacc gaaggctccc
    11161 gttacttcta tccggaattc gaccggaact cgggatttca gacccgttcc gtccttgccg
    11221 tgccgctgat ctcccaggga cggatcctgg gggtgatcga agtcttgaac aaagtcagcg
    11281 gcgaattcac ggacagcgac atgaaactcc tccagtctat cgccacctcc gtgagcatcg
    11341 ccctggaaaa cgcccggctc taccaggaga ccaaatcctt ggcggaacat gaacggggca
    11401 tccgcaacgt tttccagaaa ttcgtcccca aggaggtggt cgaaaggatc atccacggtt
    11461 ccgagaccgg ggagggcgta atggaagaga ttcgcctgtt gacgatcctc aatatagatc
    11521 tacgggggtt ctctatattg tcgtcccagt tcagccccaa ccggaccgta accattctga
    11581 accatttctt cggggtaatc ggagaaataa tcctcaaaca ccgtgggatc gtcgataaat
    11641 acctcggcga cggcctgctg ggcatcttcg gcgcccccgc atccagcggc tacgacgccg
    11701 acaacgccgt tgccgccgcc ctggagatgc aagccgccat gaagggcatc aaccatcatg
    11761 tgcacactct cgtcgaccag cccctcacga tggggatcag catccatacg ggggaggcct
    11821 tgatcgggaa catcggtttc gacaagaaga tggactacac ggtcatcggc gattccgtca
    11881 acgtagtctt ccgtctccag agtctgacca agtacaagcc cgattccatc ctcatcacgg
    11941 agatgacgca ccaggcggta gtcaagtcgg tgctccaggt ggcgccctta gaggtccagg
    12001 acgaggacat cgccaagcaa atcaacttca tgcgggttta cgaactcctc gggcaacagt
    12061 cccggcatgg atacccggaa aacgcctgac ccccgggagg gtacgccggc ccgcattggc
    12121 cccaatgaat ctctgacgac cttatcggta gaaggaaagc aaaagaaaat tgttaccggt
    12181 cactatttat ccgaagcacg gcgccgggaa aatagtgacc gaggatggtc tccatggaaa
    12241 agccccggat ggccatcatc gccgcgccga tctgacagag ccccacaccg tgaccccatc
    12301 cggccccccg gaggacgaaa cgccggatct taccccgggt gtccttctcc gcggcaacca
    12361 caaaggcact gctgtagaga tgactggggg atagccagcg gcggatttcc aactctttgc
    12421 ccacaatgac ggtgtggcgg gtgccggcga tctccaggcg gatgatccgg cccgagggac
    12481 cgcggctcag gggattcagg cgcagcagtt ccccaaaatc gattcccgat ttctctttca
    12541 gcagttcctc caattcccgc cgttcatagg caacctccca acggaaaaaa tccggcgttt
    12601 cctgatcgaa ggcgggcagg atccggcgca gtgcggtcat atcgccggta ttgcagtaga
    12661 cggccggcga cgatgagatc cagtccgcgg ccgccgcttc cgtggccagg gagggaaaat
    12721 cctccggacc gtcggcaatg gacgtcagat agggaacgga ccgttcctcc cagacattgg
    12781 caaagtcttc cgtccggccg ccgcaggcct tgtgataccg ggcgtcgcag atccggccgc
    12841 catacaccag gaccatgccc cgggtggtgt cgatggcctc ccggacccgc ggggagacga
    12901 tcttcgtcac cccctggtag cgctggcagt ggtcgtcggc gcagacatcg aaacgggtgt
    12961 gttcctcccg tccgtaccat ttgacgattt cttccgggat gtccccatcg tgatcgctat
    13021 tcactctttt ggactcccct ccccgcgccg cagccacgtc accgtccatt ccgcccgttt
    13081 ctccgccggc ggagttccgg acaacgcacc cgacagaacg tgtccggcca caaccagcgc
    13141 cggcgttctc aaggtttttc ctctccagca tggccaaaag ccagctccgg gacatgacgg
    13201 cgtgggcctt gaggaactcc accggcgccg tggcggccat ctccgaggag atgacgctgg
    13261 ccagatagtc ctcgatatcg atctcgttga tggcggtgag gaggccgtcg ggctcagcca
    13321 aagctaccaa gcttccctga aatgtctgtc gctcccgccg ttcccagtga aagttcaccc
    13381 cgatggttac ggcatccagg gaaaagtcgg cccgttcccc ggaatgaaag cggcactcct
    13441 ccggcggtag aaaaaggcgg caagtttggg tttgcagcca gagaccggtg ccttcgtcgc
    13501 cagcgccgga ggaagggggg gaatcaccgt cggcatgggg agagggcggc ccggaggtca
    13561 ggacaaaggg gccggtcaga atttctccgg cgggggtccg aaagctccca tgaaaacatc
    13621 cgcggagttc ccggcgtccc gccatgacac caacccggat tgtcttcccc cctgcggtca
    13681 tatttcccat atcctttcag tcaatgaacc gatagccttt tttctgggag aaatccatct
    13741 cctcggcaat cttcatgcac aggtcggcgg tccttctctg atgacccggc catgccatcc
    13801 acggcaaagg tggcgtcagg gagaaaatcg gtaatgtccg ccagccgtcg cggggccatt
    13861 tccttttctc aacaccgcta atcatctaca cccctttccg acaccgtcgt cggcgcggtc
    13921 atcgcgatgg ccccgacggg gcattcccgg gcgcagaagc cgcaacctat gc
//
LOCUS       DAOSMF010000039        13633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_5266_length_13633_cov_22.333702, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000039 DAOSMF010000000
VERSION     DAOSMF010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 13633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13633
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5266_length_13633_cov_22.333702"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="PLT63_05695"
     CDS             <1..810
                     /locus_tag="PLT63_05695"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOD98249.1"
                     /translation="YIDRRENREPRFDFKEMMRASPVDTYQYINIKDTIKCLADDSYF
                     HEYKPTYGPGRGDSVVCGKMWMKGIPVGVVASNASGVIYIDAARKATEWMIRCGNSNL
                     PVLFLQGSPGYMVGKAEEWGGIGKYGSDMVRACSCLMSPKVTYVIGPDHGAANYGMNG
                     RAFKPRFAFTNMRGRTTVMSGETAGFIVETVRRKNIEAKGQPVNEEEMAKFRQMMRDR
                     YDAEGHPFYTGSLLFHDGVVAFSEARDKIARAFEISLRVPVQHSDWGDLKV"
     gene            839..1639
                     /locus_tag="PLT63_05700"
     CDS             839..1639
                     /locus_tag="PLT63_05700"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOD98250.1"
                     /translation="MADILLKERTEDGILILTLNRPDAMNCFNFDLLAVLADTIREAN
                     FDMDLRCIIITGSKAGDEPKKWSFSTGADLKERRTLSQDQVRRFIFTIRNTFTAVEQV
                     RVPVIAAINGFAFGGGTELSLACDIRIASSNVLMGLTETSLAIIPGAGGTQRLPRIVG
                     VAKAKELIYTARRFNAQTALEIGLVSKVVEPDKLMDAALELAREIAKNGPIGVAQAKF
                     VLNYGTEASLGVALPLESKAYEVTIPTKDRLEALAAFAEKRKPVFKGE"
     gene            1738..2928
                     /locus_tag="PLT63_05705"
     CDS             1738..2928
                     /locus_tag="PLT63_05705"
                     /inference="COORDINATES: protein motif:HMM:NF012885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98251.1"
                     /translation="MATEYDYWKIFPRMPKKVSIGDITIRDGFQHEEKFISTKAKIFY
                     GQEMILAGCRELEVTNMAAGTTPQFVDGAEVMKAMRSDAFAKRAARQGIDVFKDVCLT
                     AVTIREPSVDLAIKWRQEGYGPDRILMMVSTDPEHHFANSGTTLTQYWREAERCIKKA
                     RDAGMKMVGTVSTIWGSPISGATKLEDAVEFTKRWFQIGAHDIEHADHDGSANAADVY
                     RYFSMVLDAMPNPEAHLCHLHETKRIASSSILAALQAGISRFEATLGGLGGQPANFLD
                     DCPIRGTGEYYYDDPRYVGLVTMEDLLVQIDELGIEHGYDVDRVLWLGRKMEKTAGRR
                     LRSEAIINGRTQKEGHMKFARPGLKKLVEKQGEQPGQRIPPDWTAEAVLPEFWGPADP
                     IWKK"
     gene            3044..3259
                     /locus_tag="PLT63_05710"
     CDS             3044..3259
                     /locus_tag="PLT63_05710"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HOD98252.1"
                     /translation="MAVDVVAPMPGTIAEILVSVGDEVQADEELIILEAMKMENPIVA
                     PKSGKVTEIKVDEKDKVDTNQVLLVLE"
     gene            complement(3352..3744)
                     /locus_tag="PLT63_05715"
     CDS             complement(3352..3744)
                     /locus_tag="PLT63_05715"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HOD98253.1"
                     /translation="MSDEIVKRTQETAKTLFGKGVKMDPPYLMWREFDKDLANEFSKF
                     ITGNLYSRKVLTLPERQYAACAILAATRATEELKLHVNAALNVGCDPKKLAEIFFQVA
                     TYAGMPAVNEALEVYREVLKERGEWPIK"
     gene            complement(3787..5106)
                     /locus_tag="PLT63_05720"
     CDS             complement(3787..5106)
                     /locus_tag="PLT63_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HOD98254.1"
                     /translation="MASTDNKRRRTGLIFFPAFDWAISPTHPEREERLLYTQDQVFEE
                     GLLDFDNISEFKPGLATVADIDRIHICVPEAKSVITESHLISAGGAITAAEKVLSGEV
                     DNAFALVRPPGHHAMRVVHGARGFCNVNIEAIMVEYIRKHYGPKRIAIVDTDCHHGDG
                     TQEIYWHDPNTLCISIHQDGRTLYPGSGFTDEFGGPNALGMTINIPLPPRTSDEGFLY
                     VLEKVIMPILAEYKPDLVINSAGQDNHYTDPITNMRFSAQGYARLNEILAPDIAVLEG
                     GYSIEKALPYVNVGIILAMAGLDYSAVREPDYHAEALRQSDQITRHIERTAAEVMQLW
                     KKRREMKKPLVGSAGYVKRNKHIYYDTDGISEQQEETVRVCDDCGGLVMIDSFADTGR
                     RVFAIIVPGQSCPVCRQEGEAFFARMEKGRHRHIYFQDRDRDIYIAK"
     gene            complement(5117..6790)
                     /locus_tag="PLT63_05725"
     CDS             complement(5117..6790)
                     /locus_tag="PLT63_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HOD98255.1"
                     /translation="MILGIDVGGTHTDAVLIEGFKIRKKAKIPTDAAHLLNSLLRVAT
                     EILTGEDPKKLQRVVLSTTLSTNAIVQGKVERVGMVVAGGPGLSPALLPRIPHTFHIT
                     GYVNHRGVIAADIDQEEIKAVGEKLRREGIKHLGVVGKFSTRNPAQELRTAALLSDNF
                     RHVSLGHRMSGHLNFRRRIDTTYLNSAVWESYQRFVNDVLQFVRSQGIEVPIYILKAD
                     GGTFDVAKSADSPVQTILSGPAASIMGILSMAKCDEDAVALDIGGTTTDIAVFADGVP
                     LLENLGVTIEGHKTLIRGLRTRSIGLGGDSAVSYEEGRLHVGPRREGPAAALGGPCPT
                     VTDAMIVLKRTAIGDGDLAVASLTPLAKQMGRSVAATAEIIFAEACGKIVAAVRSFID
                     EINNEPVYTIHEMLAGKIVAPKTLHVMGGPAAAMADALGEMLAYRPVIPQHSEVANAV
                     GAALARTTAEITILADTERRLLTIGEEGVQMEIPSRYTAAQAIAVGAEKLREKALAMG
                     AHPEDLEIEVVENQEFNMVRDMYMAGKNIRVKVQLKPGLISGFTRGQSL"
     gene            7329..7703
                     /locus_tag="PLT63_05730"
     CDS             7329..7703
                     /locus_tag="PLT63_05730"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HOD98256.1"
                     /translation="MSDRAGDFLKGVLIGGVIGAVVGILYAPKSGRETREDIGRKAEE
                     LVAKAKEEYEQALEKSRKTYETAVSRLKKIEETAREKVGDLEAKAGTMVEKGKESLED
                     NKSRLKRAIDAGVEAYKEEKSA"
     assembly_gap    7705..7804
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7853..8254
                     /locus_tag="PLT63_05735"
     CDS             7853..8254
                     /locus_tag="PLT63_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98257.1"
                     /translation="MYVELSLLLLSMAVFLMAAFTAPLLYQVHKLVKGLQVSQEMLQR
                     RLPDILKNLDEAAEQMKLTVHTVNDQVAVAAGVVKRVQAVAEVLMEVESVLRLGLRLP
                     FFVFLRNAGAVMKGVRVFLNAYSSPRRQIKG"
     gene            8268..8735
                     /locus_tag="PLT63_05740"
     CDS             8268..8735
                     /locus_tag="PLT63_05740"
                     /inference="COORDINATES: protein motif:HMM:NF008448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajD family HNH nuclease"
                     /protein_id="HOD98258.1"
                     /translation="MAKMTFGSKGGRVQAIVRKPAAGSTRPLEEVVREMKAVQQKPPG
                     EDYRERSLAIHGLVCAHCGREFSGANRQLLTVHHKDGNHHNNPPDGSNWENLCVYCHE
                     NAHSREVLGDYLAGAAAAREAEMVYQDRTEGAGSPAAGALGEKLKAALARKHR"
     gene            8920..9912
                     /locus_tag="PLT63_05745"
     CDS             8920..9912
                     /locus_tag="PLT63_05745"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HOD98259.1"
                     /translation="MDVIEQGVGRIFTDPDADKAREFFRHKSRALVSKLTTVKQAVQK
                     YVQDGDYVSIGGFGVNRIASAVLHEMVRQGKKNLGLAGHTATHDFEILSAGGCFNRCD
                     VAYIIGLEARGLSPNARQYMESGKVEVCEWTNYTLAVRYKAACAGVSFHPIRNMMGTD
                     TFKYSGAVKVKCPFTGTNYALVPALYSDVGLLHVHEADIYGNCRTKGILMSDYDIARS
                     SKKVIVTCERLITNDEIRRDPTSTFIPYWCVDAVIEQPFGCYPGNMYGEYFSDEDHLR
                     EWLKVEKDPEEFKAFLDKNIYSVKDHYEYIEKNGGMQKMLRLRQKEWLMIGARE"
     gene            9933..10703
                     /locus_tag="PLT63_05750"
     CDS             9933..10703
                     /locus_tag="PLT63_05750"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HOD98260.1"
                     /translation="MANYNTMELMICTAARELEDGASVGVGTGAPCAAAMLAQKAHSP
                     NLVILFEAGGAAPLIPEMPISVGDSRTTWKALMASSMAEIMETACRGQLDYTFLGGAQ
                     IDMYGNLNSTRIGNDHQKPKVRFPGSGGANDFGSFCWRMMVMTPQDPKRFTERCSYIT
                     TPGWLEGGDSRAKSGLPLGAGPYKIITNMAVMDFEPESKRMRIIAINPGYSEKDVQDN
                     CGFELLKAPKIVENLPPTEEELSILRDQIDPYRYVIGR"
     assembly_gap    10855..10954
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10955..12624)
                     /locus_tag="PLT63_05755"
     CDS             complement(<10955..12624)
                     /locus_tag="PLT63_05755"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HOD98261.1"
                     /translation="MTEKTKGNDFFNIYAHGFIRTAVCIPEVKVADVDFNVRATIKLA
                     RRAAANHAVFALFPELGISAYSNEDLFHQEALLLAVRDGLGKIIAASRRLRPVIVVGA
                     PLQVEDRLFNCGIAIHRGRILGVAVKSYLPNYREFYERRQFTPAEEAIVTAIDLCGQE
                     GVPFGADLIFTARNIPHCRFYLELCEDVWVPIPPSSFAALAGATLIGNLSASNITIGK
                     SDYRRSLAANQSARCVTAYLYAAAGPGESTTDLAWDGHAMIHENGNLLAESARFAPEA
                     QIIYADVDLDRLAQDRMRLTSFSQNARTHRDRVRAFRRVGFDLDVPRGMRILLAREYP
                     RFPYIPADPGKRDQRCYEAYNIQVQGLAKRLQAAGIGNVVIGISGGLDSTHALIVAAR
                     TMDLLKLPRSNIKAYTMPGFATTDKTYTNSLRLMKVLGVEANELDIRPSCLQMLEDVR
                     HPFAEGKPVYDITFENIQAGERTSHLFRLANMRRALVLGTGDLSELALGWCTYGVGDH
                     MSHYNVNASVPKTLIQHLIRWVADTVVFSPETSAVLIDILDTEISPEL"
     gene            complement(12621..13388)
                     /gene="folE2"
                     /locus_tag="PLT63_05760"
     CDS             complement(12621..13388)
                     /gene="folE2"
                     /locus_tag="PLT63_05760"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HOD98262.1"
                     /translation="MIDVQNRRDHRKIEITKVGVKGIKYPIIVLDRASGTQPVNATIN
                     MYVNLPHHFKGTHMSRFVEILNEFRGQINIKTFHVILEKVREKLRAESAHMEIFFPYF
                     IEKTAPMTKAKSLMEYKCLFCGSLNDRGTDFQVGVIVPVTTLCPCSKEISERGAHNQR
                     SIVTVKVRFRKFFWIEDLIRIVEDSASGEVYSLLKRPDEKFVTERAYENPMFVEDVVR
                     NVAARLKEHENFPSYTVEAENYESIHNHSAYAYVEGK"
     gene            complement(13385..>13633)
                     /locus_tag="PLT63_05765"
     CDS             complement(13385..>13633)
                     /locus_tag="PLT63_05765"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HOD98263.1"
                     /translation="RLNDEELLIDFRDLKRWTNEILDELDHKYLNEVEAFRDINPSSE
                     RVAKYIHDRLAPQVQAVNLRVACVTVWESDNARVAYRP"
BASE COUNT         2774 a         3816 c         4105 g         2738 t
ORIGIN      
        1 tacatcgacc ggcgcgagaa ccgggaaccc agattcgatt tcaaagaaat gatgcgcgcc
       61 agccccgtag atacctatca gtacatcaac atcaaggaca cgatcaaatg tctggccgac
      121 gacagttatt tccatgagta caaaccgact tacggacccg gacgcggcga ctccgtcgtc
      181 tgcggcaaga tgtggatgaa gggtatccct gtcggtgtcg tggcctccaa cgccagtggc
      241 gtcatctata tcgacgcggc ccgcaaggcc acggagtgga tgatccgctg cggcaattcc
      301 aacctccccg tcctgttcct ccagggttcc cccggataca tggtaggcaa ggcggaagag
      361 tggggcggca tcggcaagta cggctccgac atggtccggg cctgcagttg tctcatgtca
      421 cccaaagtta cctacgttat cggacccgac cacggcgccg ccaactacgg catgaacggc
      481 cgggccttca agccccgctt cgccttcaca aacatgcgcg gccgcaccac cgttatgtcc
      541 ggtgagacgg cgggcttcat tgtcgagacg gtgcgccgga aaaacatcga ggccaagggc
      601 cagccggtga acgaagaaga aatggccaaa ttccgtcaga tgatgcgcga ccgttacgat
      661 gcggaaggac atcccttcta taccggttcc ctcctgttcc atgacggcgt cgttgccttc
      721 agcgaggccc gcgacaagat tgcccgggct tttgaaatct ctctgcgcgt cccggttcag
      781 cactccgatt ggggcgatct gaaggtctga gttttaaacg ttaaagagga ggaaaaagat
      841 ggctgatatt ctacttaagg agagaaccga agacggcatc ctgattctta ccctgaatcg
      901 tcccgatgcc atgaattgtt tcaattttga cctcctggcg gtcctcgcgg acacgatccg
      961 ggaagccaat ttcgatatgg atctccgctg tatcatcatc accggttcca aagccggtga
     1021 cgaacccaag aagtggtcct tctccacggg agcggacctg aaggaaagaa gaaccctgtc
     1081 gcaggatcag gttcgccgtt tcatcttcac catccggaac accttcacgg ctgtcgagca
     1141 ggtgcgggtg cccgtgattg ccgccatcaa cggcttcgcc ttcggcggcg gcacggaatt
     1201 gtccctggca tgcgatattc gcatcgcctc gtccaatgtc ctcatgggtc tcacggaaac
     1261 gtccctggcc atcatccccg gcgccggcgg cacccagcgc ctgccgcgga ttgtcggtgt
     1321 agccaaggcc aaggaactga tctatacggc ccgtcgtttc aatgcccaga cggccctgga
     1381 aatcggtctg gtcagcaaag tcgttgagcc ggacaaactc atggacgccg ccctggaact
     1441 ggcgcgggaa attgccaaga acggtcccat cggcgtggcc caggcgaaat tcgttctgaa
     1501 ctacggcacc gaggcgagtc tgggcgtggc cctgccgctg gagtccaagg cctatgaagt
     1561 gacgattccc acgaaggatc gcctggaagc cctggcggcc ttcgcggaaa agcggaaacc
     1621 cgttttcaag ggagagtgat caacggatta actatttccc ctcctccatc ctgccgccgg
     1681 aggaggggaa tttaatctca acgaagcatt acattagatg aaggaggata ttgttttatg
     1741 gcgacagagt atgattattg gaagattttc ccgcggatgc cgaagaaggt ttccatcggt
     1801 gacatcacaa tccgggacgg tttccagcat gaagagaagt tcatttccac caaggccaag
     1861 atcttttacg gccaggagat gatcctcgcc ggctgccggg agctcgaggt gaccaatatg
     1921 gccgccggga cgacccccca gtttgtggac ggcgctgagg tcatgaaggc catgcggagt
     1981 gacgcattcg caaaacgcgc cgctcggcag gggatcgatg tcttcaagga tgtctgtctt
     2041 accgccgtca cgatccggga accatcggta gatctcgcta tcaaatggcg tcaggaaggg
     2101 tacggtcccg accggatcct catgatggtc tccacggacc cggagcatca ctttgccaat
     2161 tccggcacca cccttaccca gtactggcgt gaagccgagc ggtgcatcaa aaaggcgaga
     2221 gacgccggta tgaagatggt cggcacggtg agcaccatct ggggcagccc catcagcggc
     2281 gccacgaaac tggaggacgc cgtggagttc acgaaacgct ggttccagat cggcgcccat
     2341 gatatcgagc acgcggacca cgacggttcc gccaacgccg ctgacgtgta tcgttatttc
     2401 tccatggttc tcgatgccat gccgaatccg gaggcccatc tctgccatct ccacgagacg
     2461 aagcgcattg cttcttcgtc aattttggcg gccctccagg cgggtatcag ccgcttcgag
     2521 gccaccctgg gcggtttagg cggccagccg gcgaacttcc tcgatgactg tcccatccgg
     2581 ggtaccggcg agtactacta cgacgatccc cgttacgttg gtctggttac catggaagac
     2641 cttctggtgc agatcgacga gttgggcatc gagcacggct atgacgtgga ccgcgtcctc
     2701 tggctgggtc ggaagatgga gaagacggcg ggcaggcgcc tccgttccga ggccatcatc
     2761 aacggccgga cccagaagga agggcatatg aagtttgccc gtccgggtct gaagaagctc
     2821 gtcgaaaaac aaggcgagca gcccgggcag cggattcccc cggactggac cgcggaagcg
     2881 gtgctgccgg agttctgggg ccccgcggat ccgatctgga aaaagtaatt gttatcaata
     2941 acaggggcgg gaggagcggc tgcttctccc gctcactgac aagagatgct gaggcgcggg
     3001 aaaaaaccgt gcagatatta aaataatcaa ggaggttaag caaatggcag tagacgtcgt
     3061 agcccccatg ccgggaacca ttgcggagat cctggtttcg gtcggtgatg aagtgcaagc
     3121 cgatgaagaa cttatcattc tcgaggccat gaaaatggaa aatcccattg ttgccccgaa
     3181 aagtgggaaa gtgacagaga ttaaagtgga tgagaaggac aaagttgaca ccaatcaggt
     3241 cctcctggtc ctcgaatagt tactgaagaa gacagcagcg cgaaaaaaaa gacaacgccg
     3301 aatcggcata tccttagcgg ttatgccggt tcggcgtttt ttattatttg tttatttgat
     3361 cggccattcc ccgcgttcct tcaggacctc gcgatagacc tccagcgcct cgttgaccgc
     3421 cggcatcccg gcgtaggtcg ccacctgaaa aaagatctcc gccagcttct tggggtcgca
     3481 acccacgttc agggccgcgt tgacgtggag tttcagttcc tccgtcgccc gtgtggccgc
     3541 caggatggcg caggccgcgt actgccgctc cggcagggtc aacaccttcc gggaatagag
     3601 attcccggtg atgaatttcg aaaattcgtt ggccagatcc ttgtcgaact cccgccacat
     3661 caaatagggg gggtccatct tgaccccttt tccgaatagg gttttggccg tttcctgggt
     3721 tcttttaacg atctcgtcgg acataataaa actgctcctc tctcgtttta tagtttttta
     3781 ctgcgcttat ttggcgatat agatgtcgcg gtcccggtcc tgaaaataga tgtggcggtg
     3841 ccgacccttt tccatgcggg caaagaaggc ctccccctcc tgccggcaga cggggcagct
     3901 ttggccggga acgatgatgg caaagacgcg ccggcccgtg tcggcgaagg agtcgatcat
     3961 caccaggccg ccgcagtcgt cacagacccg gaccgtttct tcctgctgtt ccgagatgcc
     4021 gtccgtgtcg tagtagatgt gtttgttccg tttcacatag ccggcgctgc cgaccagagg
     4081 ttttttcatc tcccggcgtt tcttccagag ttgcatgacc tccgcggcgg tgcgctcgat
     4141 gtgccgggtg atctggtcgc tctgccgcag ggcctcggcg tgatagtccg gctcccggac
     4201 agcgctgtaa tccagacccg ccatggccag gatgatcccc acgtttacgt aggggagggc
     4261 cttctcgatg gaatatcccc cttccaggac cgcgatgtcc ggcgccagga tctcgttgag
     4321 ccgggcgtag ccctgggcgg aaaagcgcat gttggtgatg gggtccgtgt agtgattgtc
     4381 ctgccccgcg gagttgatga cgagatccgg cttgtattcc gcgaggatgg gcatgatcac
     4441 cttttccagg acgtagagga atccttcgtc ggaggtccgg ggcggcaggg ggatgttgat
     4501 cgtcatcccc agggcgttgg gtcccccgaa ctcatcggtg aatcccgatc ccggatagag
     4561 ggtccgaccg tcctgatgaa tggagatgca cagcgtgttg ggatcatgcc agtagatctc
     4621 ctgggtcccg tcgccgtgat ggcagtccgt atcgacgata gcgatccgtt tgggtccgta
     4681 atgcttccgg atgtattcca ccatgatcgc ctcgatgttg acgttgcaga agccccgggc
     4741 gccgtggacg acccgcatgg catgatgtcc cggcggccgg accagggcga aggcgttgtc
     4801 cacttccccg gaaaggacct tttccgccgc cgtgatggcc ccccccgccg agatgaggtg
     4861 ggattccgtg atgacgctct ttgcctcggg gacgcagatg tggatccggt cgatgtccgc
     4921 caccgtggcc aggccgggct tgaattcgct gatgttgtcg aaatcgagga ggccctcttc
     4981 aaagacctgg tcctgggtat agaggagcct ttcctcccgc tccggatggg tgggtgagat
     5041 ggcccagtcg aaggcgggga aaaagatcag cccggtgcgg cgccgtttgt tgtccgtaga
     5101 tgccattatt ttttcctcac agggactgtc cccgggtgaa gccggagatc agccccggct
     5161 tgagttgaac cttgacccgg atattctttc ccgccatgta catatcccgg accatgttga
     5221 attcctgatt ctccacgact tcgatttcca gatcctccgg atgggcgccc atggcgaggg
     5281 ccttttcccg cagtttctcc gcgcccaccg cgatggcctg cgccgccgta taacgcgaag
     5341 ggatttccat ctgcaccccc tcttcgccga tggtgaggag ccgacgctcc gtgtcggcaa
     5401 ggatggtgat ttccgccgta gtgcgggcca aagcggcacc cacggcgttg gctacctcgg
     5461 agtgctgggg gatgacggga cggtaggcga gcatttcgcc caaggcatcc gccatggccg
     5521 ccgccggtcc tcccatgacg tggagggttt tcggcgcgac gatctttccc gccaacattt
     5581 cgtggatggt ataaacgggt tcgttgttga tttcatcgat gaaagaacgt accgccgcga
     5641 caatctttcc gcaggcctcg gcgaagatga tctccgccgt cgcggcgacg gagcggccca
     5701 tctgctttgc cagaggcgtc agggaggcaa cggccaggtc cccatccccg atcgcggtcc
     5761 gcttcaggac gatcatggcg tcggtgacgg tggggcaggg cccgcccagg gccgccgccg
     5821 gtccttcccg ccggggaccc acgtgcagcc gtccctcctc gtaactgacc gcgctgtcgc
     5881 cgcccaggcc tatggacctg gtccgcaatc cccggatcag cgtcttgtga ccttcaatgg
     5941 taacgccgag gttttccaac aggggcacgc cgtcggcaaa gacggcgata tccgtggtgg
     6001 tgccgccgat gtccagcgcc acggcgtctt cgtcgcattt ggccatgctg aggatgccca
     6061 tgatactggc cgccggcccg gagaggatgg tctggacggg agaatcggcg gatttggcca
     6121 cgtcgaacgt tcccccgtcg gccttgagga tatagatcgg gacttcgatg ccctggctcc
     6181 gcacgaactg aaggacgtcg tttacaaagc gctggtagct ctcccagacg gcgctgttga
     6241 ggtaggtggt atcaatgcgg cgccggaaat tcaggtgccc cgacatgcgg tgccccaagg
     6301 acacgtggcg gaaattatcc gacaggagag cggccgtccg cagctcctgg gcggggttgc
     6361 gggtggagaa tttgcccacg acgcccaggt gcttgatccc ctcccgccgg agcttttccc
     6421 ccaccgcctt tatctcttcc tggtcaatat cggcggcgat cactccccgg tggttgacgt
     6481 aaccggtgat atgaaaggtg tggggaatcc ggggaaggag cgctggagac aggccgggac
     6541 cgccggcgac caccattccc acccgttcca ccttgccctg gacaatagcg ttggtggaga
     6601 gggtggtgct gaggactacc cgttgcagct tctttggatc ttccccggtg aggatctccg
     6661 ttgccacccg gagcagggag ttgaggagat gggctgcgtc cgtcggaatc ttggcctttt
     6721 ttctgatttt gaatccttcg atcagaaccg catccgtatg cgttccgcct acgtcaatgc
     6781 ctaaaatcat gattcctccg gtttgcggcc tcgaatttgc catgccttat ataatgttaa
     6841 atttatcttg acaaggtaaa aaaaaggcgt atggtctctc atcactcaag gtgattagag
     6901 tcatcgtatg gcaatgaaat cattggaaag gaggtgacgc tttaatgaag aaggtattcg
     6961 ccattattct ttcgctgctt ttggttgtga cgttttcgtt tgcggtagtt ggttgcaaga
     7021 aggcggaaga gcagaaaccg gctgaaccgg ccaagacggc agcccccgct cccgctccgg
     7081 cggctcctgg tgcggctccg gcggctcctg gtgcggctcc ggcggctcct ggtgcggctc
     7141 cggcggcacc cggcgcggcc ccggcggccc cggctcccgc agctccggcg gcaaagtagt
     7201 ataaaatcaa cagatcaaac ggaaaaccgg atcgtcgctt tgcgaaggtc cggttttttt
     7261 atgtctcaaa aggccttgac agctttccgg caattgctta ataaaaagaa aaaaatagga
     7321 ggtgaactat gtcggaccgt gcgggagatt tcttgaaggg agtgctcatc ggtggggtaa
     7381 tcggcgcggt ggtggggata ctttacgccc cgaagagcgg ccgggagacc agggaagaca
     7441 tcggccgcaa ggcggaggag ctggtcgcca aagccaagga agaatacgag caggccctgg
     7501 aaaagagccg gaagacctat gaaaccgcgg tcagccgcct gaagaaaatc gaggaaacgg
     7561 ctcgggagaa ggtgggagat ctggaggcca aagcgggaac catggtggaa aaggggaagg
     7621 aaagcctgga agacaacaag agccggctga aaagggcgat cgacgccggg gtggaggctt
     7681 acaaagagga aaaatcggcc taagnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7801 nnnnatccgt catcatcgac aaaggcaata aaatccagat gcgaggattg aaatgtatgt
     7861 agagctcagc ctgttattgc tcagcatggc cgtttttctc atggcggcgt ttaccgcccc
     7921 cttgctctat caggtgcaca aattggtgaa aggcctgcaa gtctcccagg agatgctcca
     7981 gcggcgcctg cccgacatcc tgaaaaacct cgacgaggca gcggagcaga tgaagttgac
     8041 ggtccatacc gtcaacgatc aggtagccgt ggccgcgggc gtcgtgaaga gggtgcaggc
     8101 agtggccgag gtgctcatgg aagtggagtc ggtgctccgg ctggggctcc gcctgccctt
     8161 tttcgttttc ctcaggaatg ccggtgctgt catgaagggc gtccgggtct ttctgaacgc
     8221 ctattcctct ccccggcggc agataaaagg atagggggtt ccggggcatg gctaaaatga
     8281 ccttcggcag caaaggcggg cgcgtccagg cgatcgtacg gaaacccgcc gccgggtcca
     8341 cccggccgct ggaagaagtg gtccgggaga tgaaagccgt ccaacagaag cccccgggcg
     8401 aggattaccg ggagcgttcc ctggccatcc atggcctggt atgcgcccac tgcggccggg
     8461 aattttccgg agcgaaccgc cagctcctga cggttcatca caaggacggc aaccatcata
     8521 acaaccctcc cgacggcagc aactgggaga atctctgcgt ttattgtcac gaaaacgccc
     8581 acagccgcga ggtgctgggc gattatctcg ccggtgccgc tgcggcccgg gaggcggaaa
     8641 tggtctatca ggatcggacg gaaggggcgg gatctcccgc tgccggagct ctgggagaga
     8701 agctgaaggc cgccctggca aggaaacatc ggtgacaata aaccgaatat tttccttgct
     8761 tcccgccgtt tgttgtgcta agacgccact gactcatcgc agttagttaa acccctttaa
     8821 ttatttttgt attcagcggt aatgtttacg tacggcacag gcgtgtaata aagtaaacag
     8881 gtaaattgtc acaaaaaaag agagaaggag gtaggttcag tggacgttat tgaacaagga
     8941 gtaggaagga tctttactga tccggatgcg gacaaagcga gggaattttt ccgccacaaa
     9001 agccgcgccc tcgtgagtaa actcaccacg gtcaaacaag cagtccagaa gtatgtccag
     9061 gatggcgatt atgtttcgat cggcggcttc ggagtcaaca ggatcgcgtc ggcagtgttg
     9121 catgaaatgg tcagacaggg gaagaagaac ctgggattgg ctggccatac cgcaactcac
     9181 gacttcgaga ttctttccgc gggaggatgt ttcaacaggt gcgatgttgc ttacatcatc
     9241 ggcctcgaag caagaggctt gtcccccaat gcccggcaat acatggaaag cggcaaagtg
     9301 gaagtgtgcg agtggacaaa ttataccctg gccgtgcgct ataaggcagc ctgtgcggga
     9361 gtttctttcc atcccatccg caatatgatg ggcaccgata ccttcaaata cagcggtgcg
     9421 gtaaaggtta aatgtccctt tacaggtacg aattacgctc tcgtaccggc tctgtattcg
     9481 gatgtaggtc tgcttcacgt ccatgaggcg gacatttacg gcaactgccg gaccaaaggc
     9541 atcctgatgt cggattacga cattgcccgt tcctctaaaa aagtgatcgt aacctgtgaa
     9601 cgtttgatca ccaacgacga gatacgtagg gatcccacct cgacctttat tccttactgg
     9661 tgtgtcgatg ccgttatcga acagcctttc ggctgctatc ccggtaacat gtatggcgag
     9721 tatttctccg atgaagatca tctccgtgaa tggttgaagg tggagaagga tccggaagag
     9781 ttcaaggcgt tcctcgacaa gaacatttac agtgttaagg atcactatga atacatcgag
     9841 aaaaacggcg gcatgcagaa gatgcttaga ctcagacaga aagaatggct catgatcggc
     9901 gccagggaat agaaaggagg agggaagaga ttatggcaaa ctacaataca atggaattga
     9961 tgatctgcac cgcggcgagg gaactcgaag acggcgcttc cgtgggcgtc ggcaccggcg
    10021 ctccctgtgc cgcagccatg ctggcgcaga aggcccattc accgaatctg gtgatcttgt
    10081 ttgaagcagg cggcgctgcg cccctgatcc ctgaaatgcc catctccgtc ggtgattccc
    10141 ggacgacctg gaaggccctc atggccagca gcatggcgga aattatggaa accgcctgcc
    10201 gcggacagct cgactataca ttcctcggcg gggcgcagat cgatatgtac ggcaacctga
    10261 actccacgcg gatcggcaac gatcaccaga agccgaaggt ccgcttcccc ggcagcggcg
    10321 gcgccaatga ctttggatcc ttctgctggc ggatgatggt catgaccccc caggatccca
    10381 agcgcttcac cgagaggtgt tcctacatca ccacccccgg ctggctggaa ggcggcgatt
    10441 cccgcgccaa gtccggtctg cccctgggag ccggccccta caagatcatc acaaacatgg
    10501 ccgttatgga tttcgaacca gaatccaagc ggatgcggat cattgcgatc aatcccggct
    10561 attccgagaa ggacgtgcag gacaactgcg gcttcgagct cctcaaggcc ccgaagatcg
    10621 tggagaacct tcccccgacg gaagaagagc tgagtatcct ccgcgaccag attgacccct
    10681 atcgctacgt gatcgggcgc taaacaccga ttcgaccacc gccggcgggt tataccccgg
    10741 ctcgacggtg agggagaaga tggactccgg gcttgcccgg ccggaaatct tcgctcgctc
    10801 caggctgaaa aaacaaaggc cgccgcccca tccgggacgg cggccttgtt cttcnnnnnn
    10861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnatgagt tccgggctga tctccgtgtc
    10981 gaggatgtcg atgagcaccg ccgacgtctc cggcgaaaac accaccgtgt cggccaccca
    11041 gcggatcaga tgctggatca gggtcttggg gacgctggcg ttgacgttgt agtgggacat
    11101 gtgatcgccg acgccgtagg tgcaccaccc cagggccagc tcgctcagat cgcccgtgcc
    11161 caataccagg gcccggcgca tgttggccag ccggaagagg tgggaggtcc gctcccccgc
    11221 ctggatgttt tcaaaggtga tatcgtaaac cggctttccc tccgcgaagg ggtgccgaac
    11281 atcctcgagc atctggaggc agctcgggcg gatgtccagc tcgttggcct ccacccccag
    11341 gaccttcatc agccggaggg aattggtgta ggtcttgtcc gtggtggcga agcccggcat
    11401 ggtgtaggcc ttgatgttgc tccggggcag cttgaggaga tccatggtcc gggccgctac
    11461 gatgagggcg tgggtggaat ccaggccgcc ggagataccg atgaccacgt tgccgatccc
    11521 cgccgcctgg agccgcttgg ccaggccctg gacctggatg ttgtaggcct cgtaacatcg
    11581 ctggtcgcgc ttgccggggt cggcggggat gtaagggaat cgggggtatt cccgggccag
    11641 aagaattcgc atgcctcggg ggacgtcgag atcgaaaccg acccgccgga aagcccgcac
    11701 ccggtcgcgg tgggtccggg cgttctggct gaagctcgtg agacgcatcc ggtcctgggc
    11761 cagccggtcc agatcgacgt cggcgtagat gatctgcgcc tccggggcga agcgggccga
    11821 ttccgccagg agattgccgt tttcgtgaat catggcgtgg ccgtcccagg ccagatccgt
    11881 cgtcgattcc cccggccccg ccgccgcgta tagataggcg gtgacgcaac gggccgactg
    11941 gttggccgcc aggctccggc ggtagtccga tttgccgatg gtgatgttcg acgccgagag
    12001 gttgccgatg agcgtcgccc cggccagggc ggcgaaactg gagggcggga tggggaccca
    12061 gacatcctcg cagagctcga ggtagaagcg gcagtggggg atgttacgtg ccgtgaagat
    12121 cagatccgcc ccaaagggga cgccttcctg accgcagagg tcgatggccg tgacgatcgc
    12181 ctcctccgcg ggggtgaact ggcggcgctc atagaattcc cggtagttgg gcaggtagga
    12241 cttgacggcg acgccgagga tccggccccg gtggatggcg atgccgcagt tgaagaggcg
    12301 gtcttccacc tgcaaggggg cgccgacgac gatcaccggc cggagccgcc gggaggcggc
    12361 gattattttc cccagcccgt cccggacggc cagcagcagg gcctcctgat ggaagaggtc
    12421 ctcgttggaa taggccgaga tccccagctc cgggaagagg gcgaagacgg cgtgattggc
    12481 cgccgcccgc cgggccagct ttatcgtggc ccggacgttg aaatccacat cggccacctt
    12541 cacctccggg atgcaaacgg cagtgcggat aaagccgtgg gcgtagatgt tgaagaagtc
    12601 attccccttc gttttttccg tcattttccc tccacatagg cataggcgct gtgattgtgg
    12661 atgctctcgt agttctccgc ctccacggtg taggagggga aattctcgtg ttccttcagg
    12721 cgggcggcga cgttccggac cacgtcctcg acgaacatgg gattttcgta agcccgctcc
    12781 gtgacgaatt tctcgtcggg gcgcttgagg agggaataga cctcgccgct ggccgaatcc
    12841 tccacgatgc ggatgagatc ctcgatccag aagaacttcc ggaaccgtac cttgacggtg
    12901 acgatgctgc gctggttgtg ggcgccccgt tcgctgatct ccttggagca ggggcacagc
    12961 gtcgtcacgg gaacgatgac gcccacctgg aagtccgtcc cccggtcgtt cagggagccg
    13021 cagaagaggc atttgtattc catgaggctc ttggccttcg tcatcggcgc cgtcttctcg
    13081 atgaaatagg ggaagaagat ctccatgtgg gccgattcgg cccggagctt ctcccgcacc
    13141 ttctccagga tcacatggaa ggttttgatg ttgatctgcc cccggaattc attgagaatt
    13201 tccacgaagc ggctcatgtg ggtccccttg aaatgatgag gcaggttgac gtacatgttg
    13261 atcgtggcgt tcaccggttg ggtgccggag gcccggtcca ggacgatgat cgggtatttg
    13321 atccccttga cgcccacctt ggtgatctcg atcttccggt gatcccggcg gttctggacg
    13381 tcgatcatgg ccggtacgcc acccgggcgt tgtcggattc ccagaccgtc acgcaggcca
    13441 cccgcagatt cacggcctgc acctggggcg ccaaccggtc atggatgtat ttcgccaccc
    13501 gctccgacga ggggttgata tcccggaagg cctccacttc attgagatat ttgtggtcaa
    13561 gctcgtcgag gatctcattg gtccaccgct tcagatcccg gaaatcgata aggagttcct
    13621 catcgttgag gcg
//
LOCUS       DAOSMF010000040        13544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_5314_length_13544_cov_23.009267, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000040 DAOSMF010000000
VERSION     DAOSMF010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 13544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13544
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5314_length_13544_cov_23.009267"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(528..1565)
                     /locus_tag="PLT63_05770"
     CDS             complement(528..1565)
                     /locus_tag="PLT63_05770"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOD98264.1"
                     /translation="MPFDFDSLREYLLPVAFLAGGVIGGLIVEKIVLYELRKLTNRTK
                     GELDNLAIRSLHGMPLLWLTLAGIYGAINNVDMRPGLLSFLQQALLVAAIASLTWATA
                     RFLVGFVNHYSAKVGGPLLSTSMFANLTRIIVFIVGGLIILQSLGVSVTPLLTALGVG
                     SLAVALALQDTLSNLFAGIHIIASQQVRPGDYVQLDSAQEGYVTDITWRYTTIRALPN
                     NMVIIPNSKLASAIVTNFYNPETQMAVRVPVGVNYASDLDRVEEISLAVARETMREVP
                     GGIPDFEPVLRFHTFGESSIDFNVIMRCREFTDQFVVRHEFVKRLHRRYRAEGIEIPF
                     PIRTVHLQKEK"
     gene            complement(1671..2390)
                     /locus_tag="PLT63_05775"
     CDS             complement(1671..2390)
                     /locus_tag="PLT63_05775"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOD98265.1"
                     /translation="MGVTLDSSGLRRGVFLSLGVHALAALILAGGLAGSTPPRPIKGF
                     DSCLHVDLVSTARAPAHPTPHPAPPEAVRRIKPVPAPVPTPVAAPADHPRSESLRAAA
                     VLASLARRLPLGHDGSSAGVGSGEDGTPPPAAPSGPAGGKETSGTETSLAVPRYHENP
                     HPAYPRLARLRGHEGVVLLAVEVLSNGKVGRLRVKTSSGHALLDQSALAGLKKWTFEP
                     GRKLGVPVAMWVDVPVRFHLQ"
     gene            complement(2451..4721)
                     /locus_tag="PLT63_05780"
     CDS             complement(2451..4721)
                     /locus_tag="PLT63_05780"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOD98266.1"
                     /translation="MNRSLVWWLGLASLVLVAALGSTPAFSWEGARRPSLQMEFPRAV
                     RVGFFDLASRSDAEPKASTPLENAGKASSATAAGEKTPVASPGVKSRGEAPALRMEEV
                     VVTATRDWEEVRKVPADVSVITEADIKKSGATSLVEVLEKLEGIQIRSYSGQSPQSMV
                     DLRGFGGENPFGKTLVLIDGRRQNRPDMSSNNWFQIPLGSIERVEVVRGAGTVLYGDN
                     AIGGVINIITKKGKGKPAVTVAATAGSYGLNNERIGITGRQDKLSYSVNGENYFSWGY
                     RERSKLTSQSAGFELGYELGEHFQLSLGMSGNRSDYNLPGALTKKQMDQDRRQYQPAT
                     PENWTNAAPDDDGRDRTGRINLGATAVHESLGQLDLLFGYGSNAYEFNMPSWNFNSYT
                     NTDMDSVSFTPKYTLEKGILGFQNKLTAGLDYYNEPYRKTAWDSRERQVKTGWAELRR
                     KTLEYYVRDEFSPWKRLILAAGYRAGDTTLGGDHTDALTPANSFSNQEKRHPAEAWEG
                     SATVLLGKKSNLYGKYARIYRIPFLDEQASFNGWGGGFYTNLEKEQGQSMEVGTLLYP
                     LEALKVSLSLFRIDMEDEITWNNATKRNENLDRTRHQGIEGSFSWNIKGIVTLNGQYT
                     YHMATFEDGQYNKKELPLVPNRIMGAGADIKLPFSFTLRPEVRYVSDCYLSGDYDNNA
                     EKLSSFIVYNLFLFFERRQPLGKHFLNIRAFAAVENINGEKYAGFGTDNDSWGGLNTY
                     YPMPERTFKGGLSLEF"
     regulatory      complement(4752..4947)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(5005..5790)
                     /locus_tag="PLT63_05785"
     CDS             complement(5005..5790)
                     /locus_tag="PLT63_05785"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD98267.1"
                     /translation="MTLMKIDQLGFRYGDPWVLRGVTFSLAQGDFIGVLGPNGSGKST
                     LLRLLAGILTPQEGEALLEGRPVHTMKRRDVARRIAVVPQSYEGIFAFSAREVVLMGR
                     SPHLPAWSFEAPHDFAVVERVMEQTDTRELAARPLDQLSGGERQRVLIARALAQEPRL
                     LLLDEPTAFLDIRHQVEILNLIKTFNKKEKLAVVAVTHDINLASLYCDRIILLDRGVV
                     HDDGVPEAVLTVGNIECVYGTPVLTSRHELTGRPVVAPRTEIW"
     gene            complement(5787..6842)
                     /locus_tag="PLT63_05790"
     CDS             complement(5787..6842)
                     /locus_tag="PLT63_05790"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOD98268.1"
                     /translation="MGAFSLPTSYLAVTPARVVRISLLLCLLLVAVALASLFTGTADI
                     SIRATLREFFPWASFLSGGLTEAETSIIMSVRLPRIILAAIVGASLSVAGVVFQALLR
                     NPLADPYILGISAGSAVGAVMGILLGVGAWSLGIPGLAFLGALAAILIVFGIAGREKR
                     RETNTLLLTGVMVNAFFNAFILFVFTISDHVQLQRALNWIMGDLSLADEQALFYTSVF
                     LVLGSVFIYAFARPLNLLVTGEETAQQLGVAVEKTRIALLAAAALITAAAVSASGTIG
                     FVGLIIPHILRLLVGPDHRLLIPTAFFTGGAFLVVADTLARCLVAPAELPVGVITALC
                     GAPYFLLLLRRRLRLTS"
     regulatory      complement(6855..7020)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(7085..7942)
                     /locus_tag="PLT63_05795"
     CDS             complement(7085..7942)
                     /locus_tag="PLT63_05795"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HOD98269.1"
                     /translation="MKTNRHFFFKAIIFVLLPHLLGVSGAAASPPRRIVSLAPNITEI
                     IFGLGLGDRLVGVTTYCDFPQAARKKHKIGGFSNPSLEAILALRPDAVILTEDGNPPE
                     IAPRLERLGISIHVFRARKIEELPGALREMGRFLQVEAAARRQAAEMERRLKDYRSSA
                     RQRRRAANPKALFIIQPEPLIVAGPGTPMDDAMNIMGLANIADDVTGRYPKYSLEETL
                     RRQPQLLFIGQGPGMEEDVRRLSRRLRTLEAVRLGRVYTIRETLFRLGPRVFIGLEEM
                     KAAVVQSGL"
     gene            complement(7929..8939)
                     /locus_tag="PLT63_05800"
     CDS             complement(7929..8939)
                     /locus_tag="PLT63_05800"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOD98270.1"
                     /translation="MKRKRLYCPHCGGDIRREWEEDVERDFCAACRTFFYENPLPVVS
                     VILLSADREILLVKRGRRPYRGRWCLPTGFVESGESIETAALRELAEETGLRGRVAGL
                     VDVDSGRNSFYGDLIFLTFEAEAEGETGPPQSGGDTVAARYFPLGGLPRLAFPANNRA
                     VKRFVAAKADYWAIVDSFSLAAGGDGATGGRNTNLLSDRLVRVIEVNAELISRLWIEE
                     VTTSRSTPGYHHFDRQRLFRRVHTILSQFGKWLGGGYDDRDIQAYYTDMGRERRREGF
                     RLGEILSALSLIKKHLWEFALSRGMWQKTIDIYMALELDRRIVIFFDKASFYTARGYE
                     DK"
     gene            complement(9011..9871)
                     /locus_tag="PLT63_05805"
     CDS             complement(9011..9871)
                     /locus_tag="PLT63_05805"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOD98271.1"
                     /translation="MNKEAQQEQIAAVFTGVDAHLVTAELIRSHSTNREDIREVALRG
                     LDLRPCRRILDLGCGFGFFSEALRNRVGPGARLTGLDMIPAYERLFLDVCRRIGVRGR
                     FIAAPVSHVETLPERSWDLVLCSYALYFFPEITVRIARLLAAGGIFIAITHGEKNMGE
                     LFTAARTVLKEQGIMNRERLPVEEIIGRFCAENGGELLRRSFHRIDTIDYPNTLIFKP
                     HELPLLLAYFHFKSPFFLAGIDALHRDRIMTTFAAHLEDVTRRQEGFTIFKDDRIFIC
                     REPRDNADGP"
     gene            10219..10644
                     /locus_tag="PLT63_05810"
     CDS             10219..10644
                     /locus_tag="PLT63_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98272.1"
                     /translation="MAKSIKAAKAVTATKAVKATKKTKKRDDVDRVFELTAEELPEKW
                     KDKLESFFEETEAGIHDHWHLRSEEVGSIDVYHTFGQFDITNLIEGNIRVFLKGRAIK
                     MRGQESRVFRIEEGYIISPAIYENTSCFRYALPAEGEPA"
     gene            10812..12335
                     /locus_tag="PLT63_05815"
     CDS             10812..12335
                     /locus_tag="PLT63_05815"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD98273.1"
                     /translation="MEVFTLATVSLAIAVFLFFTKRQNRTHLSFAWLCLAVFLYKGGG
                     FFSGHFHSDVWNLIELCGLMAIPPLLMSFGRRLLNDQSLLSPRRTATATFTSVALFIS
                     LFTPLADWPYWRMLIYLYPAAILALCYGALILFIRNRPAGTERTRMLYLSIAFGMAAV
                     ISTLDVVKFFGYDFYPLYNIFLAVLMYMIFLIITHPQLMELHEVMARALITGVLTLCA
                     TLVIYVFIRLFGHPSSSSLTYILLASYLIVISIEPLKQVLKNIFGWVYPESKDAFNFL
                     YAFDRKLEREKSMLLEEMAPVLAHEIRNPLGSIKGAAQYLRSEVLNEEDRRLIDVIIA
                     EVNRLNSVVNQFLSYAKPYQLNLKLQDVNAVITKAISIVRANDLTERIVIEEELRSEL
                     PPMNIDAEQLIQVILNIAFNALEAMPTGGKLIFRTRRIDSDLGEAVGISIRDTGKGIK
                     KEELRNVFKPFFTTKERGVGLGLAICQRIIRSHGGHIRVKSLPGQGSIFYIRLQKAS"
     gene            12460..>13544
                     /locus_tag="PLT63_05820"
     CDS             12460..>13544
                     /locus_tag="PLT63_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOD98274.1"
                     /translation="MNRILVVDDEINMQVVLRAMLKKEGYEVFTAPDGREALKVLADD
                     DIDVVVTDLKMPNLDGMGLLERVAADYPAVPVIMITAHGTVATAVDALKKGAFDYVTK
                     PFEQDELKNVVLKAIKTRRLSDEEFVAGADEIDRYGIVGASEPMQEIFETIKRVAPTT
                     TTILISGETGTGKELIANAIHINSPRKGNPLIKINCAAIAENLMESELFGYEKGAFTG
                     AVVTKPGRFELAHKGTLFLDEVGELPKEMQVKLLRVIQEQTFERVGGLKTIKVDVRLI
                     TATNRNLFQDVKEGRFREDLFYRLNVLPIHLPALRERRDDIPTLIAYFVDRFRKKLGR
                     DIQGLEDDVLDLFINYDWPGNIREMENL"
BASE COUNT         2809 a         4065 c         4052 g         2618 t
ORIGIN      
        1 agcgccctct acggcgccga cgtccgcatg gtaaaccacc gccacgggct gcaaacagta
       61 aaataatcaa tgatcccggc ggcattttga acgataccga agggggatat ccccatcaaa
      121 ctggacccga ccgtgtaacc tgaatctgaa tatattcctg gaaaatattg ggagacatta
      181 ttctcgattc gaggcagagg atattcaccc cccccataat taggtgccta ctccataaca
      241 ccttgaaata acgagattat tattcagaaa aaccccggag ccctacggct gcattgggtt
      301 ccattttcgc ttaataatat caagaagtta aatgctatcg gattaagata atttttttgc
      361 cacccccagc ggcgccggca atatggtccg gattgcgaaa caacgggctt ggtacggcgg
      421 cccgggggcc gatgcctgag acccgccggt ggcgaagcgt gaaacgatga aaactctaat
      481 ggaataaatg atttgaagag gcgtagatgg tgtttccccg atgtatgtca cttctccttt
      541 tgcaggtgca ccgtacggat gggaaagggg atctcaatgc cttcggcacg gtagcgccgg
      601 tgcaacctct tcacgaactc gtggcggacg acgaactggt cggtaaactc ccggcagcgc
      661 atgatgacat tgaagtcgat gctggactcg ccgaaggtgt ggaagcgaag gaccggttca
      721 aaatcgggta tgccgccggg gacctcgcgc atggtctccc gggccaccgc cagggagatc
      781 tcctccaccc ggtccaggtc gctggcatag ttcaccccca ccggaacgcg gacggccatc
      841 tgggtttccg ggttgtagaa gttggtgacg atagccgagg ccagtttgga attcgggatg
      901 atcaccatgt tgttgggcag ggcccggatg gttgtataac gccaggtgat gtctgtgacg
      961 tacccctcct gggcactgtc caactgcacg taatcccccg ggcggacctg ctgggaggcg
     1021 atgatgtgaa tccccgcaaa gagattggaa agggtgtcct ggagggccag ggccacggcg
     1081 aggctgccca cccccagggc cgtgaggagg ggcgttacgg aaacgccgag ggattgaagg
     1141 atgatcaaac cgccgacgat aaaaacgata atccgcgtca ggttggcgaa catggatgtg
     1201 gaaaggaggg ggccgcccac cttggcgctg taatggttca cgaagcccac gaggaaccgc
     1261 gccgtcgccc aggtgaggga ggcaatcgcc gccacaagga gcgcctgctg taggaagctc
     1321 agaagacccg gccgcatgtc cacattgttg atggcaccgt agatcccggc cagggtgagc
     1381 cagaggagag gcatgccgtg gagggaccgg atggcgagat tgtccagttc ccctttcgtc
     1441 ctgttcgtca gtttcctgag ctcgtaaagg acgatctttt ccacgatcaa ccctcctatc
     1501 accccaccgg ccaggaaggc cacggggagg aggtattccc gtaaagaatc gaaatcaaag
     1561 ggcatatatc gcttttcctt tttttatcgc ctcgtatgga catagtcaac gagacgggca
     1621 aagtcaaggg aaaacgggcg ggccggcggc ggaaaaggga accggcgctc ctattgcaaa
     1681 tggaagcgga cggggacgtc cacccacatg gcgacgggaa cacccagctt ccggccgggt
     1741 tcgaaggtcc actttttcag gccggcgagg gccgattgat ccaggagggc atgtccggaa
     1801 gaggtcttga ccctgagtcg ccccaccttg ccgttggaca gtacctccac ggccaggagg
     1861 accaccccct catggccccg gaggcgggcc aggcgcgggt aagccgggtg gggattctcg
     1921 tgataccgcg gtaccgccag ggaggtctcg gtcccgcttg tctctttacc ccccgccggt
     1981 ccgctcggag ccgccggcgg gggggtgccg tcttctccgg acccgacccc ggcgctgctc
     2041 ccgtcatggc cgaggggcag gcgccgcgcc agggaggcca ggaccgccgc cgcccgcaac
     2101 gattcgctcc ggggatgatc ggccggcgcc gcgaccggtg tcggcaccgg cgccggcacg
     2161 ggctttatcc tccgtaccgc ctccgggggg gcgggatgcg gcgtcgggtg agcaggcgcc
     2221 cgcgccgtgg aaacgagatc gacatggaga caggaatcga atcccttgat cggacggggc
     2281 ggcgtcgagc cggccaaccc gccggcgagg atcagggccg ccagggcgtg gacccccagg
     2341 gacaggaaga cccccctccg gagtcccgag ctgtctaaag tgactcccaa cctcaaatcc
     2401 gtttgctctt gttccaaccc attcactgtg ttatcaaacc gctcccatgt tcaaaattcc
     2461 agggacagcc cgcccttgaa ggttcgctcc ggcatgggat agtaagtgtt caagccgccc
     2521 caggagtcgt tgtccgtacc gaaacccgca tatttttcac cgtttatgtt ttctacggcg
     2581 gcgaaggccc ggatgttgag gaaatgtttc cccaggggct gccgtcgctc gaagaagagg
     2641 aaaagattat agacgataaa agaggaaagt ttctccgcat tgttgtcgta atcgcccgag
     2701 agatagcagt cgctcacgta gcggacctcc gggcggaggg tgaaggaaaa gggcagcttg
     2761 atgtcggcgc cggcccccat gatccggttg ggaaccaggg gaagttcctt tttgttgtac
     2821 tgaccgtctt cgaaggtcgc catgtggtag gtgtactgtc cgttcagggt tacgatcccc
     2881 ttgatattcc aggaaaacga tccctctatt ccttggtggc gggtccggtc gaggttttcg
     2941 ttgcgctttg tggcgttgtt ccaggtgatc tcgtcctcca tgtcgatgcg gaacagcgac
     3001 agggagacct tcaaggcttc cagggggtac aggagggtcc ccacctccat gctctgcccc
     3061 tgttccttct ccaggttcgt atagaaaccg ccgccccagc cgttgaaaga ggcctgttcg
     3121 tccagaaagg ggatccggta gatccgggcg tatttgccgt aaagattgga tttctttccc
     3181 aacaggaccg tcgcgcttcc ctcccaggcc tcggcggggt ggcgtttttc ctgattggaa
     3241 aagctgttgg ccggggtcag ggcgtccgta tggtcgccgc cgagggtggt gtccccggcc
     3301 cgatagcccg ccgcaaggat caggcgcttc cagggggaaa attcgtcgcg gacgtaatac
     3361 tccagcgtct tccgccgcag ttccgcccag cccgtcttta cctggcgttc ccggctgtcc
     3421 cacgcggtct tgcgatacgg ctcgttatag taatccaatc cggcggtcag tttgttctga
     3481 aaaccgagga tgcctttctc cagggtgtac ttgggcgtga aggagacgga atccatatcg
     3541 gtgttggtgt acgagttgaa attccacgaa ggcatgttga actcataggc attactgccg
     3601 taaccgaaga ggaggtccaa ctgcccgagg gattcgtgaa cggccgtagc ccccaggttg
     3661 atccgccccg tccggtcccg gccgtcgtca tccggggcgg cgttggtcca gttttccggt
     3721 gtcgcgggct ggtactggcg gcggtcctgg tccatctgct tcttggtcag ggccccgggg
     3781 agattgtagt cgctgcggtt tcccgacata cccagggata actggaaatg ctcgccgagc
     3841 tcgtatccga gctcgaaccc cgcggactgg gaggtgagtt tcgagcgttc ccggtagccc
     3901 caggagaagt agttttcccc gttgaccgag taggacagct tatcctgcct tcctgtgatg
     3961 ccgatccttt cgttgttgag gccgtaactg cccgccgtag ccgccactgt gaccgccggt
     4021 ttgcccttgc ccttcttcgt gatgatgttg attaccccgc cgatggcgtt gtcaccgtag
     4081 aggaccgtac cggccccccg gaccacctcc acccgctcga tgctccccag ggggatttgg
     4141 aaccagttgt tggaactcat atccgggcgg ttctggcgcc ggccgtcgat gaggaccagg
     4201 gtcttgccga aggggttctc ccctccgaaa cccctcagat ccaccatgga ctggggggac
     4261 tggccgctgt aagagcggat ctgtattcct tccagcttct ccaggacctc caccagggag
     4321 gtcgccccgg atttcttgat atccgcctcg gtgatcacgg agacatcagc gggaaccttc
     4381 cgcacctcct cccagtcccg ggtcgccgtg acgaccacct cctccatgcg gagggccggc
     4441 gcctcccccc ggcttttcac tccgggcgac gcaacgggcg tcttctctcc ggcggccgtc
     4501 gcgctgcttg ctttacccgc attttccagt ggtgttgagg ctttcggttc agcatcgctg
     4561 cgcgacgcta aatcaaaaaa cccgacccgg accgcccgcg ggaattccat ctgtagcgac
     4621 ggtcgtcgcg ccccttccca ggaaaaagcc ggggtggacc ccagtgccgc aaccaagacc
     4681 aacgacgcca aacccaacca ccaaaccagc gatctgttca tgattcctcc ttcttccccc
     4741 gcgggagaaa agaaatgttc gcttcgtcag gcaagtttcc tggctcgggg ctcacaccta
     4801 ctcgccgctc cttcccggcg gcccttccgg ccgccagtgg tctgccgcgg ttttcgttcc
     4861 ccttacagtt gcggggcagc gccggcctca caccggcttc cttcccccga cgaagtaata
     4921 tccgtgacta catcttcgag aatgcttaca tcttcctcaa tatccacttg ctgcatcgca
     4981 ccggtacaac ggcacggccg ttcttcacca aatctccgtg cgtggagcga cgacgggccg
     5041 ccccgtaagt tcatgacggc tcgtcaatac cggagtccca tagacacact cgatattccc
     5101 caccgtgagg accgcctccg gaaccccgtc atcatgcacc accccccggt ccaggagaat
     5161 gatccggtcg cagtataggg aggcgagatt gatatcgtgg gtgacggcga ccaccgccag
     5221 tttttctttt ttattgaagg tcttgataag gttgagaatc tccacctgat ggcggatgtc
     5281 gaggaaggcc gtgggctcgt cgaggaggag gagccggggc tcctgggcca gggcgcgggc
     5341 gatgaggacc cgctgccgct ccccgccgct gagctggtcc aggggacggg cggccagttc
     5401 ccgagtatcc gtctgttcca taaccctttc cactaccgcg aaatcatgcg gcgcctcgaa
     5461 tgaccaggcg gggagatggg gcgaccggcc catgaggacg acctcccggg cggaaaaggc
     5521 gaagatcccc tcatagctct ggggaacgac ggcgatgcgc cgggccacat cccgcctttt
     5581 catggtgtga acgggacggc cctccagcag cgcctctccc tcctggggcg tgaggatgcc
     5641 agccagcagg cggagcaacg tcgattttcc cgacccgttg ggaccgagga ccccgatgaa
     5701 atccccctgc gcaagggaga aagtaacccc ccggaggacc caggggtcac cataacgaaa
     5761 acccagttgg tcgatcttca tgagggtcat gacgtcaacc gcaacctccg gcggagcaac
     5821 agcagaaaat atggggcacc acacagggcg gtgatgaccc ctacgggcag ttccgccggc
     5881 gccacgagac accgggccag ggtgtcggcg acgacgagaa aggccccccc cgtaaagaaa
     5941 gcggtgggga tgaggaggcg gtgatccgga ccgacgagga ggcggaggat gtgggggatg
     6001 atgaggccga cgaagccgat ggtgccgctg gcggaaacgg cggcggcggt gatcagggcg
     6061 gcggcggcca aaagggcgat cctggtcttt tccacggcca cgcccaactg ttgcgccgtt
     6121 tcctcgccgg tgacgaggag gttcagggga cgggcgaagg cgtagataaa aaccgacccg
     6181 aggacgagaa aaacggaggt gtaaaacagg gcctgttcat ccgccaggct cagatcgccc
     6241 atgatccagt tgagggcccg ctggagctgc acatggtcgc tgatggtgaa gacgaagagg
     6301 atgaaggcgt tgaaaaaggc gttgaccatg acgccggtca aaagcagggt gttggtttcc
     6361 cggcgttttt cccggccggc gatgccgaag acgatcagga tcgcagccag ggcgccgaga
     6421 aaggccagac cgggaatgcc caggctccag gcacccaccc ccaggagaat tcccatgacg
     6481 gcccccaccg cggaaccagc ggatattccg aggatatagg ggtccgccag gggatttctc
     6541 aacagggcct gaaacaccac gcccgccacg gagagggatg ccccgacaat ggcggccagg
     6601 atgatccggg gcaggcggac agacatgatg atagacgttt cggcttcggt caggccaccg
     6661 gacaggaagg aggcccaggg gaagaattcg cggagggtgg cccggatgga gatatcggcc
     6721 gtcccggtga aaagggacgc cagggccacg gcaacgagaa gcaggcacag cagcagggag
     6781 attctgacca cccgcgccgg cgttacggcg agatatgatg tcggcagtga aaacgcgccc
     6841 aatgcttccc ctcccccgag gtcaaacaga tgggccggtc ttccggctcc gggcgcccta
     6901 ctcgccgcct tcccgtcccc cggtggagac agtggcattc cggctttcgt tcccgttacg
     6961 gctgcggggc agcgggggat ttgcacccct cttccacaca cccatctggt ctggcaagat
     7021 ttacgcgatg ataattaccg aaagaatttc cttatgtcaa ggggcaaatt cactacctcg
     7081 ttttttacaa accgctctgc accaccgccg ccttcatctc ctccaggccg atgaacaccc
     7141 gggggccgag gcggaaaagg gtttcgcgga tcgtgtaaac ccgtcccaac cggacggctt
     7201 ccagggtccg aagccgtcgg gaaagcctcc gaacgtcttc ctccatcccg gggccctggc
     7261 cgataaacaa aagctgcggt tgcctccgga gggtctcctc cagggagtat ttcgggtagc
     7321 gcccggtcac atcatcggcg atattggcca gccccatgat gttcatggcg tcgtccatgg
     7381 gggtgccggg cccggccacg ataaggggct cgggctggat tatgaacagg gcctttggat
     7441 tggcggcccg gcgccgctgc cgagccgacg accggtaatc tttcagccgg cgctccattt
     7501 ccgccgcctg ccgccgggcg gcggcctcca cctggagaaa acgtcccatt tccctgaggg
     7561 ccccggggag ttcctcgatc ttccgggccc ggaagacatg gatggagatc cccagtcttt
     7621 ccagccgggg ggcgatctcc gggggattgc cgtcctcggt gaggattacc gcatcggggc
     7681 ggagggccag gatcgcctcc agggaggggt tggaaaaacc gccgatcttg tgctttttcc
     7741 gggcagcctg gggaaagtcg cagtaggtgg tcacccccac cagccggtcc cccagtccca
     7801 gaccgaagat gatttccgtg atgttcgggg ccagggagac gatccgccgg gggggggacg
     7861 ccgccgcccc ggaaacgccc aaaaggtgcg gcagcaggac gaaaatgatt gctttaaaaa
     7921 agaaatgcct atttgtcttc atagccccgg gcggtgtaaa aagaggcctt gtcgaagaag
     7981 atcacgatgc ggcggtccaa ctccagggcc atgtagatgt cgatggtctt ctgccacatc
     8041 ccccgggaaa gggcaaactc ccagaggtgt ttcttgatga ggctcagggc actcaggatc
     8101 tctcccaatc ggaatccctc ccgccggcgt tcccggccca tgtctgtgta gtaggcctgg
     8161 atgtcccggt cgtcatatcc cccgcccagc cattttccga actgggagag gatggtgtgg
     8221 acccggcgga agaggcgttg ccggtcgaaa tgatggtagc cgggggtgga gcggctggtg
     8281 gttacctctt cgatccagag gcgggagatg agttccgcat tgacctcgat gacccgaacg
     8341 agacggtcgg aaaggagatt cgtgtttcgc ccgccggtgg cgccgtcccc gccagcggcc
     8401 agggaaaagg aatcgacgat ggcccagtag tcggccttgg cggcgacaaa acgcttcacc
     8461 gcccggttgt tggccggaaa ggccaggcgg ggaagccccc cgaggggaaa atagcgggcc
     8521 gccacggtat cgcccccgga ttgcggaggc cccgtctccc cctccgcctc tgcctcgaag
     8581 gtcagaaaga tcagatctcc gtaaaagctg ttccgccccg aatccacatc caccagaccg
     8641 gcgacacgcc cccggagtcc cgtttcctcc gccagctccc gcagggcggc cgtttcgatg
     8701 ctttccccgc tttccacaaa ccccgtcggc aggcaccacc gtccccggta cggccggcga
     8761 ccccgtttaa ccagcaggat ctcccgatcc gccgacagca gaatcacgga caccaccggg
     8821 aggggatttt catagaagaa ggtccggcag gcggcacaaa aatccctctc cacgtcctcc
     8881 tcccactccc ggcggatgtc gccgccgcaa tgggggcagt acaggcgctt tcttttcacc
     8941 ggcgtctcct atatcaatgt gaatttctta atgaatccat agggcagcat cgggggctca
     9001 ccccttgttt tcacggccca tccgcgttgt cccggggctc ccggcagatg aagatgcggt
     9061 cgtccttgaa gatggtgaaa ccctcttgcc gccgggttac gtcctccaga tgggcggcaa
     9121 acgtggtcat gatcctgtcc cggtgcagtg cgtcgatccc ggcgagaaag aaggggctct
     9181 tgaagtgaaa gtaggccaaa agcagaggca gttcatgggg tttgaaaatc agggtgttgg
     9241 gatagtcgat agtgtcgatg cggtggaaag agcgcctgag tagctctccg ccgttttccg
     9301 cgcagaagcg gccgatgatc tcttccacgg gcaagcgctc ccggttcatg atgccctgtt
     9361 ccttcaggac ggtcctagcg gcggtgaaca gttcccccat gttcttctcc ccatgggtga
     9421 tggcgatgaa aatccccccg gcagcgagga gccgggcaat ccgcaccgtg atctcgggaa
     9481 agaaatacag ggcgtaacta cacaggacaa gatcccagga acgctcggga agggtttcga
     9541 cgtgcgagac cggggcggcg ataaagcgtc cccggacccc gatgcggcga cagacatcca
     9601 ggaagaggcg ttcgtaggcg gggatcatgt ccagaccggt caggcgggcg ccgggcccca
     9661 cccggttccg gagggcttcg gaaaagaatc cgaagccgca gcccaggtcg agaatccgcc
     9721 ggcagggtcg caaatccagt ccccgcaggg ccacctcccg aatgtcttcc cggttggtgg
     9781 aatgggaccg gataagctcc gccgtcacca agtgggcatc gacgccggta aaaaccgcgg
     9841 cgatttgctc ctgctgcgct tccttgttca taactcccta ccgtattaag gacgattgcc
     9901 ggtaaagcat aggtcctttt tccaggaccg tcaaatattt atttggctga cacgacggat
     9961 cagagtgggt gcgaaatcat cttcaccctg cgaaggatgc tacgcattta tgacctgcca
    10021 ccggggccgt gccatttgcg cccaccgaag actccttgcc aataaatgca actacatcat
    10081 atacatagga catattgacg gctaaaaagc cgccctggca tacattgtga tagatatcat
    10141 gcaggacttc ggccgcccat aaacaacagc gtacagagta acaactatgg acatactcaa
    10201 tagaaagagg aggcaatcat ggcgaaatca ataaaggcgg caaaggcggt caccgcgacc
    10261 aaagccgtaa aagcgaccaa aaagacgaaa aaaagagacg acgtggatcg ggtcttcgag
    10321 ctgaccgcgg aggaactccc ggagaagtgg aaggacaaac tcgagtcgtt cttcgaggaa
    10381 accgaagcgg ggattcacga ccactggcat ctccggtccg aggaagtcgg cagcatcgat
    10441 gtgtatcaca ccttcggtca gttcgacatc acgaacctca tcgaggggaa tatcagggta
    10501 ttcctcaagg gacgggccat caagatgcgg ggccaggagt cccgggtctt ccggatcgaa
    10561 gagggataca taatctctcc ggcgatctat gaaaacacca gttgtttccg ttatgccctg
    10621 ccggcggagg gagagccggc ctaagatctt tgccgtcgac actaatccgt tgtcatcaac
    10681 ggaggtaaac aggagctgtt ccaagggggg gggaagctcc tgttttttta tggtctttct
    10741 tgactacaga ttcatttccc gttattcttc agcggcgggt ttatattcag gacatatcat
    10801 cttggagatc aatggaagtc ttcaccttag ccaccgtcag cctggcgatc gccgtttttc
    10861 tcttttttac caaacggcag aaccgcaccc atctatcttt tgcctggctt tgcctggccg
    10921 tttttctcta caagggagga ggtttctttt ccggtcactt tcactccgac gtctggaatc
    10981 tgatcgagtt gtgcggcctt atggccattc ccccgctgct catgtccttc ggacggcgcc
    11041 tcctcaacga tcagagcctc ctttctccgc gccggaccgc cacagcgacg ttcaccagcg
    11101 tggccctttt catctccctg ttcacccccc tggcggactg gccttattgg cggatgctca
    11161 tttatctcta tcccgccgcc attttggccc tctgctacgg tgccctgatt ctctttatcc
    11221 ggaaccggcc ggcgggaacg gagagaaccc ggatgttgta tctctccatc gctttcggga
    11281 tggccgccgt catcagcacc ctggatgtgg tcaaattttt cggttacgat ttttatccgc
    11341 tgtacaacat ctttctcgcc gtactgatgt acatgatttt tttgattatc acccatcccc
    11401 agctcatgga actccacgag gttatggccc gggcgctgat caccggcgtg ctcaccctgt
    11461 gcgccaccct ggtcatctat gtcttcatcc gcctcttcgg gcatccatcc tcatcgtccc
    11521 tcacctacat cctcctggcc tcgtatctca tcgtaatttc aatcgaaccc ttgaagcagg
    11581 tcctcaaaaa catcttcggt tgggtctatc cggaaagcaa ggacgccttc aatttcctct
    11641 atgccttcga ccggaaactg gaaagagaga agagcatgct tctggaggaa atggccccgg
    11701 ttttggccca cgagatccgc aatcccctgg gctccatcaa gggggccgcc cagtacctcc
    11761 gttcggaggt gctgaacgaa gaggaccggc gcctgatcga cgtcatcatc gcagaggtga
    11821 accgcctcaa cagcgtcgtc aaccagttcc tgagctatgc gaaaccctac cagctcaatc
    11881 tgaagctcca ggacgtgaac gccgtgatca ccaaagccat ctccatcgtc cgggccaacg
    11941 acttgacgga acggatcgtc atcgaagagg agctgcgttc ggagctgccc ccgatgaata
    12001 tcgacgcgga gcagctcatc caggtaatcc tgaatatcgc cttcaatgcc ctggaggcca
    12061 tgccgacagg aggcaagctc atcttccgca cccgtcggat cgacagcgac ctgggagagg
    12121 cggtgggcat ctccatccgc gacaccggca aagggatcaa gaaggaagag ctaagaaatg
    12181 tcttcaaacc ttttttcacc accaaggagc ggggagtcgg cctgggattg gccatctgcc
    12241 agcggatcat caggagccac ggcggccata tccgggtcaa gtccctgccc ggacagggga
    12301 gcatcttcta tatccggctc caaaaggctt cctgacgctc ccgccgggcg gcctttcttc
    12361 ccgaccccat gagggattgc gggctggtca aaacgtcctt ttagagataa ataataggga
    12421 cgattataat cgataataca aagctgccaa ggcgagggga tgaacaggat tctggttgtc
    12481 gacgacgaaa tcaacatgca ggtggtcctc cgggccatgc tgaaaaagga aggttacgag
    12541 gtattcaccg ccccggacgg ccgcgaggcc ctcaaggttc tggctgatga cgacatcgac
    12601 gtggtcgtca cggatctcaa gatgcccaac ctggacggca tgggccttct ggagcgggtg
    12661 gcggccgact atccggcggt ccccgtaatc atgatcaccg cccacggaac ggtggccacg
    12721 gcggtggacg ctctgaagaa aggggcgttc gattacgtaa ccaagccctt cgagcaggat
    12781 gaactgaaaa acgtagttct caaggccatc aagacgcgcc ggctgagcga cgaggaattt
    12841 gtcgccggcg ccgacgaaat cgaccgctat ggcatcgtcg gcgccagcga gccgatgcag
    12901 gagatcttcg agaccatcaa gcgggtggcc ccgacaacca ccaccatcct catctccggc
    12961 gagacgggaa cgggcaagga actcatcgcc aacgccatcc acatcaacag cccgcggaaa
    13021 ggcaaccccc ttatcaagat caactgtgcg gccattgcgg aaaacctcat ggaaagtgag
    13081 ctattcggct atgaaaaggg ggcgttcacc ggggcggtgg tgaccaagcc gggacgtttc
    13141 gagctggctc acaagggcac cctctttctc gacgaagtag gggaactgcc caaggagatg
    13201 caggtgaagc tcctgcgggt catccaggag cagaccttcg agcgggtcgg gggactcaag
    13261 acgatcaagg tggacgtgcg cctcatcacg gccaccaacc ggaacctctt tcaggacgtc
    13321 aaggaggggc gtttccggga ggacctcttc taccgcctca atgtcctgcc cattcacctg
    13381 cccgccctgc gggagcggcg ggacgacatc cccacgctga tcgcgtattt tgtggaccgg
    13441 ttccgtaaga agctcggccg ggacatccag ggtctggaag acgacgtcct ggatctgttc
    13501 atcaattatg actggccggg caacatccgg gagatggaaa acct
//
LOCUS       DAOSMF010000041        13536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_5318_length_13536_cov_23.793710, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000041 DAOSMF010000000
VERSION     DAOSMF010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 13536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13536
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5318_length_13536_cov_23.793710"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            279..875
                     /locus_tag="PLT63_05825"
     CDS             279..875
                     /locus_tag="PLT63_05825"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOD98275.1"
                     /translation="MAINLPDLPYGKDALTPYIGATTLEFHHGKHHRAYVDNANKLLA
                     GTALENATPEEIIKKTAGDPAQTAIFNNVAQAWNHAFYWRGIKPNGGGPPTGTIAGKI
                     AATWGGYDKFVEEFKNAGVTQFGSGWAWLVLEGGTLKIVKTGNADNPLTKGMKPLLTI
                     DVWEHAYYLDYQNRRPDYLATFIDKLINWDFVNANLDR"
     gene            872..1564
                     /gene="radC"
                     /locus_tag="PLT63_05830"
     CDS             872..1564
                     /gene="radC"
                     /locus_tag="PLT63_05830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOD98276.1"
                     /translation="MRDEESAPHYLGHRQRLKEKYLTCGLEGFHDYEIMELLLTYGIP
                     RKDVKEPAKELLKRFGGLKGILDAGIDELREVRGITAHTAVLVKLVKEISAAYLLQKA
                     QERPQISCTAELIDFCKTAMGALKDEEFRVCYLDAQNRIIAVETVQEGIVNQAVVYPR
                     KVLERALQWKASAIILVHNHPSGHVRPSDADIRLTRTIQETARTLDILVHDHLIIGEN
                     RFFSFREEGLMA"
     gene            complement(1561..2367)
                     /locus_tag="PLT63_05835"
     CDS             complement(1561..2367)
                     /locus_tag="PLT63_05835"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOD98277.1"
                     /translation="MIHSTLFVLTLLTTLIAGAIQQGINLLETPGGIWHGIPFSFTLL
                     IILAAHEFGHYFMARHHQVDVTLPYFIPAPSFIGTFGAFIKMRSPLLDRRMLLDIGAA
                     GPVAGLAVAMPVIVVGFALSEVRPVPAEAGMSLGSSLLFSLLGWIVHGSQPRDMDIVL
                     HPVAFSGWIGLLVTCLNLLPVGQLDGGHVAYAVLGRRQRFLARMIFVMLIILGITSWS
                     GWLIWAAILYIMGLDHPPVIYEWVPLDRRRRVIGWASMGIFFLTFTPAPF"
     gene            complement(2494..2769)
                     /locus_tag="PLT63_05840"
     CDS             complement(2494..2769)
                     /locus_tag="PLT63_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOD98278.1"
                     /translation="MNKSGLIEALSRKENITEKKAVEVVNLIFKGFTEELKKGGRIEI
                     RGLGSFVVRDYEAYTGRNPKTGKNIKVSPKKLPFFKVGKELKEKVDN"
     gene            complement(2868..3656)
                     /locus_tag="PLT63_05845"
     CDS             complement(2868..3656)
                     /locus_tag="PLT63_05845"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOD98279.1"
                     /translation="MVKDAEDSATPSLTGERLQKILAGVGISSRRAAEAMIRQGRVTV
                     NDQTVSEMGFRVRRDDAIRVDGKLIGPRPEHVYLMLYKPRGYVTTLNDPQGRPIVTDL
                     IPEQTGRVFPVGRLDYDSEGLLLLTNDGDWAYRLQHPRFRLEKTYHIKIKGNISRAAM
                     RRLEKGLQLDDGPFHPLRFEVGKVNDRSCWIDITISEGRNRILRRAFKQLELPVVRLI
                     RTAVGPLNLAGLTEGQSRFLDPREAAACLIPQVRRSIKKYSKIT"
     gene            complement(3725..4252)
                     /gene="scpB"
                     /locus_tag="PLT63_05850"
     CDS             complement(3725..4252)
                     /gene="scpB"
                     /locus_tag="PLT63_05850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOD98280.1"
                     /translation="MNENKAIIEGLIFAAESPLTIERIIEILQEREINRQEVAVHLRE
                     LMAAHEERRGGFLLREIAGGYQFRTRPDLAVWIAKMKAGRPAALTAAALETLAVVAYR
                     QPVVKAEVDRLRGVDAGRPLRTLLERRLIRILGRKDVPGKPMIYGTTKKFLEVFGLKD
                     LSELPTLGELKDLTE"
     gene            complement(4245..4970)
                     /locus_tag="PLT63_05855"
     CDS             complement(4245..4970)
                     /locus_tag="PLT63_05855"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HOD98281.1"
                     /translation="MDYEVKLDIFEGPLDLLLHLIKKNELDIYDIPIALITRQYLEYI
                     EALKALNLDLAGEYLVLAATLVHIKSKMLLPETTEEGEEELDPRRELVEQLLEYQSFK
                     NASQSLQARELLDRDVFARGSAAVTEALPQVEPPWVEIGLFELVDAFRQVLGKLSSPE
                     RMEIDLDRTSLADRINTIMERLQCHRSLTFAELLEERTDRHLIIHTFIALLELVRQRL
                     IRIFQAGPFGVIRVFPAVEEEHE"
     gene            complement(4960..5961)
                     /gene="trpS"
                     /locus_tag="PLT63_05860"
     CDS             complement(4960..5961)
                     /gene="trpS"
                     /locus_tag="PLT63_05860"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOD98282.1"
                     /translation="MTKKRILSGMRSTGKLHLGNYHGALKNWVELQNDGAYECFYFVA
                     DWHALTSDYADTSEIAENIMDMVIDWLSAGLDPDRSTLFVQSQIKEHAELWLLLSMIT
                     PLPWLERNPTYKEMKEELSSKDLSTFGFLGYPVLQAADIIMYKAYGVPVGVDQLPHIE
                     LTREIARRFNFLYREVFPIPEPLLTQVPKLLGIDGRKMSKSYENSIYMSDTGEALQNK
                     VASMFTDPQRQRRRDPGRPELCNVFTFHQLYTDWEKTREIETECRQAGIGCTDCKKIL
                     GKNIGAAMLPLHERQAYYRDHLQEVRDIVAAGDLRARSVAERTLEEVRAAMKISYGL"
     gene            complement(6113..7318)
                     /locus_tag="PLT63_05865"
     CDS             complement(6113..7318)
                     /locus_tag="PLT63_05865"
                     /inference="COORDINATES: protein motif:HMM:NF015523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HOD98283.1"
                     /translation="MKRLPHTCFHLLLLLLPTLLFIASCRPVSDLSRDDSPRIPPSSQ
                     VQEGTVPFDDLDLPSLEKAIAVSIQYYRSRGAEETHCLRDRCYSAVEMISGLESFLKI
                     MKSDLSAVAKERVIRAEFEIVAADEDTVFTGYYEPILNGSLTRTERCRYPLYRIPEET
                     VVIRLDRFEGTWGQGRLIGRLDKGEVLPHFSRRQIDSQGVLAGRGLEIAWVDDPVDLF
                     SLHIQGSGKIRLADGRQIAVNYAQSNGRPFRGLTGMMASRGLLAEGERSYEQMKRVLR
                     ERPEERQELMNYNQRYIFFRIVPEGPLGALGLTLVPERSIATDLDLYPKGALAMIETR
                     KPVFADDGRILSWQPFSRLVLNHDTGAAIKGPGRVDLFCGTGPVAERTAGAMKEKGRM
                     FFILKRSDQ"
     gene            complement(7315..7773)
                     /locus_tag="PLT63_05870"
     CDS             complement(7315..7773)
                     /locus_tag="PLT63_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HOD98284.1"
                     /translation="MLRKARIGDVKTIHKMINVSASKEEILPRSLMDIYGSLRDFFVY
                     YDDEEGLITGICAMSIIWENLAEVRSLYVDETFRRRGIGRILVEACISEAITLELFRI
                     FTLTYKREFFQRVGFHAVNRSSLSEKIWTDCFRCPKYPDYCDEVAMIIEL"
     gene            complement(7773..9455)
                     /gene="recN"
                     /locus_tag="PLT63_05875"
     CDS             complement(7773..9455)
                     /gene="recN"
                     /locus_tag="PLT63_05875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOD98285.1"
                     /translation="MLAELSIRNFAIIEALDITFSNGMNILTGETGAGKSIIMGAVSL
                     LLGDRASVDMIRSTADTATVEARFLVSDQHGIRARLAGWGLEAGDEMVVRRVISRGGK
                     NRVYINGNLASLAMLAELGESLLNICGQHEHQLLLKEDHHLDILDAFGGLQGLRGDYE
                     VIYNRYQVLKERRRTLEIRQRQREEREEFLRFQHAEIEAAALETGEDGHLAEEKQILT
                     NIRKLTDYAVTAHEILYGREHSLLKELRIVRNAVGEIKKIDPRLTVTEAQLDDIYYHL
                     EDAAYTLRNYAQGLAGDDQRLEQIETRIELINKLKRKHGATLAAVLEKKDALERELDG
                     IASLGRELEETDSSLQTVRRELEAKAGELSAARQVAAGLLQKDIEREIHALRMEAAVF
                     SVVFQDNGNGEDTFTPRGTDRLEFYLSANPGEVPKPLSRVASGGELSRLVLAMKNVLA
                     RVASVGTMVFDEVDSGIGGATAEIVGKKIRAVAERHQVLCITHLPQIACYGDRHYLVT
                     KNVGENRTETQVRELGEEARVEEIARMLSGLDITVTTKKHAREMLARAGRRE"
     gene            complement(9499..10377)
                     /locus_tag="PLT63_05880"
     CDS             complement(9499..10377)
                     /locus_tag="PLT63_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOD98286.1"
                     /translation="MRKVGIVANTAKDKAGEVTQKLRDWCLEHGIEVYLDKGISASIG
                     DGPGYDRRQLAVRADIIAVFGGDGTMLRTARAVREFSVPIVGINLGGFGYLTEVNLNE
                     MIDAFEIFRCQGFRTEKRMMLDITIESGGEVVKKRTVLNDVVINRGNLSRIVEMETAV
                     DGCYLNTFKADGLIVSTPTGSTAYSLSAGGPIVFPQHDSIIINPICPHTLTNRPVILP
                     GDVNIRVALWSKEQGATVTLDGQIFHTLKSGDVMTVRKSEYVTLLVVSPHRDYMEILR
                     SKLGWRGLTAGIQRPG"
     gene            complement(10484..11446)
                     /locus_tag="PLT63_05885"
     CDS             complement(10484..11446)
                     /locus_tag="PLT63_05885"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HOD98287.1"
                     /translation="MIATRVTEIFGIRYPIVQGGMIWVSGWRLAAAVAKAGALGLIGA
                     GSMTPDLLREHIRKIKKEGIEGVWGVNLPIFFQYAEEMAQIVLEEKVRVVFTSGGSPR
                     KYISRFKERGIKVAHVTATPELAVKCEEAGVDAVVVEGFEAGGHNGRDELTTMVLVPQ
                     SVDAVKIPVIAAGGIFDGRGMAAALAMGASGVQIGTRFAATRESSAHENFKRMMLEAS
                     PTDTFLILRKLIPARVIKNAWYERVVEAEKRGAGEEELLRIIGEKRTKMGMFDGDLVE
                     GELQIGQAAGAIRDLPAAGEVVNAIARDCEQIMAEMPGRLLRNQ"
     gene            complement(11655..12368)
                     /locus_tag="PLT63_05890"
     CDS             complement(11655..12368)
                     /locus_tag="PLT63_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HOD98288.1"
                     /translation="MHKDFESFCRRFKAAAAGKEDAENAVAAGIPLVQELLSSDLWWR
                     DFIREVLAERTWFKQQPPTLWPNEITLYRSPDQSFLILAYIWESYSVDTIHDHGSWGI
                     VGSYINKIREVKYLRLDDGAVAGHAELEATEEKVLKPGEITTVFPLDGGIHQMENASG
                     DIAVTINVYGRTVRRGYLQFFDRERSTVRRVYPPRTFKEVLMVRTLAAMGGAADILSE
                     AMGNSLPDYVREEYCQALG"
     gene            complement(12558..13385)
                     /locus_tag="PLT63_05895"
     CDS             complement(12558..13385)
                     /locus_tag="PLT63_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HOD98289.1"
                     /translation="MKKEELSYNLKYFCQEAGADIVGFAPVERWIEEGIIPLEFRPQG
                     IWSPARTVVVLGLGMPLPIVETTPSVMHMELYKTVNRKLDALAYDVTRHLNRLGHVAT
                     FFSRDGYSSLKDLRRWNGAAFGHVQAAKYAGLGTIGRNHCLLTPEFGPRVRLVSIFTA
                     AALPPDPLVEKELCIKCEACVKCCPKEALRVRDDRIPGDYDKTACLEMAEELTSRRSY
                     PCGICIKVCPVGADRKLYQQKGMMKKYLREREVLVADPDHVDYRSWQHIRRYGVKEP"
BASE COUNT         2953 a         4046 c         3510 g         3027 t
ORIGIN      
        1 gacgtcatcc tgcagatcaa ccgggtgcgg atccaatcct tgcaggatta caccggcgaa
       61 ctcgcccgga gcgaaaaaga ggaaacggtc atgttcctgg ttaagagagg ggacggggcg
      121 atgttcgtca ccctgcgccg ggaacagaaa aagtagacgg aggggagttt ccgccggtcg
      181 gaccgagatc agaggggatg atatcccccg tcaatcatca ccttgattgt ccgttcttgc
      241 ttcgttatat taaacctata aaagaaagga gaacctctat ggccatcaat ttacctgacc
      301 ttccctacgg aaaggacgcc ttgacaccct acatcggcgc caccaccctg gagttccatc
      361 acggcaagca tcatcgggcc tacgtggaca atgccaacaa actcctcgcc ggtacggcgc
      421 tggaaaatgc caccccggaa gagatcatca aaaaaaccgc cggtgatccg gcccagaccg
      481 ccatcttcaa caacgtcgcc caggcctgga accatgcctt ctactggcgg gggatcaagc
      541 ccaacggcgg cggaccgccg accggaacta tagccggcaa gattgccgcc acctggggag
      601 gctacgacaa gttcgtggag gagtttaaaa acgccggcgt aacccagttc ggcagtggat
      661 gggcatggct tgtcctcgaa ggggggacgc tgaagattgt caagaccggc aatgccgaca
      721 atcccctgac caaaggaatg aagcccctgc tcaccatcga tgtctgggag cacgcctact
      781 acctggatta ccagaaccgg cggccagact acctggcaac cttcatcgac aaacttatca
      841 actgggattt cgtgaacgca aatttggatc gatgagggat gaagagtccg cgccccatta
      901 tctgggacac cggcagcgtc ttaaggaaaa atacctgacc tgcggtctgg aggggtttca
      961 tgattatgaa atcatggaac tcctcctcac ctacgggatc ccccggaagg acgtcaagga
     1021 accggccaaa gaactcctca aacgcttcgg cggcttgaag gggatcctgg acgccggcat
     1081 tgacgagctt cgggaggtgc gggggatcac cgcccacacc gccgtcctgg tcaaactcgt
     1141 caaggaaatc agcgctgctt atcttctcca aaaggcccag gaacggcccc agatctcctg
     1201 caccgccgaa ctcatcgact tctgcaaaac cgccatggga gcgctgaaag acgaggagtt
     1261 ccgagtatgc tatctcgatg cccaaaaccg catcatcgcc gtggaaaccg ttcaggaggg
     1321 catcgtcaac caggccgtcg tatatccccg caaggtcctg gaaagggcac tccagtggaa
     1381 ggcatcggcg atcattctgg tccacaacca tccttccggc catgtccgac cctccgacgc
     1441 ggacatacgt ctcacccgga ccatccagga aaccgcccgg accctagata tccttgtcca
     1501 tgaccacctc atcatcgggg aaaaccgctt cttcagcttt cgggaagaag gcctgatggc
     1561 ctaaaaggga gcgggggtga aggtaaggaa gaaaatcccc atggatgccc agccgataac
     1621 ccgccgccga cggtccaggg gcacccattc atagatcacc gggggatgat ccagacccat
     1681 gatatagagg attgccgccc agattagcca tcctgaccag cttgttatcc ccaggatgat
     1741 caacatcacg aagatcatcc gcgccagaaa ccgctgccgc cgacccagga cggcatacgc
     1801 cacatgacca ccatcgagct ggccgacggg caggagattg aggcaggtga ccagtagtcc
     1861 gatccagccg gaaaatgcga cgggatggag gactatgtcc atgtcccggg gctggctgcc
     1921 gtggacgatc cagccaagga gggagaagag gagggaactg cccaaactca tgccggcttc
     1981 cgcaggcacc ggacgaacct ccgagagggc aaatcccacc acaatcaccg gcatggccac
     2041 cgccagaccg gccacgggtc cggcggcgcc gatatcgagg agcatgcgcc ggtcgaggag
     2101 gggggacctc atcttgataa aggccccgaa ggtgccgatg aacgacgggg ccggaatgaa
     2161 ataaggcagt gtcacgtcta cttggtgatg ccgggccata aaataatggc cgaattcgtg
     2221 agcggctagg atgatcagca gcgtaaatga gaaaggaatt ccgtgccaga tgccgccggg
     2281 ggtttccagg aggttgatcc cctgctggat ggccccggca atgagggtcg taagcagggt
     2341 cagaacaaag agggtggaat ggatcatgta aaaatggcaa ccaaccaata caaaaaggga
     2401 tcgccctgaa aaacggcatg ttgaaggccg cttcgggatg atccctccat gttaaaaaca
     2461 gtgggtagga cctgttaccg gtcccggctg aagttagtta tctacctttt ctttcaactc
     2521 cttaccgact ttgaaaaatg gcagcttctt gggagacacc ttgatattct tgcctgtctt
     2581 ggggttccgg ccggtatagg cctcgtaatc gcgtaccacg aaacttccca gaccccgaat
     2641 ttctatcctg ccccccttct tcaattcttc ggtaaacccc ttgaagatca gattaaccac
     2701 ttcaaccgcc tttttctcgg tgatattctc ttttctgctc aatgcttcaa tcaacccgga
     2761 tttgttcata tactcctccc tgagaattgc ctgtaacgct cgtcatttct aataacatga
     2821 taatatggct ggtttttcgg cgactattat ttattttgtt ttgaatgtca agtaattttt
     2881 gaatattttt taatacttct ccgaacttga ggaatcagac acgcggccgc ctcccgaggg
     2941 tcaagaaacc tgctctgccc ttccgttagt cccgccaggt tcagcggacc caccgccgta
     3001 cggatgagtc ggaccacggg cagttccaat tgcttaaagg cccgtctcag aatgcggttg
     3061 cgcccctcgc tgatggtgat gtctatccag cagctcctgt cgttcacctt tcccacctcg
     3121 aaacgcaacg ggtgaaaagg accgtcgtca agttgcagtc ccttttccag tcggcgcatc
     3181 gcggccctgg aaatatttcc tttgatcttg atgtggtagg tcttctccag gcgaaaacgg
     3241 ggatgctgca gccgataagc ccagtctccg tcattggtga gcaacaggag tccctcggaa
     3301 tcgtaatcga gccttcccac gggaaagacc ctcccggttt gttcgggaat caggtcggta
     3361 acgatcggtc ttccctgggg atcattaagg gtggttacat atccccgggg tttgtacaac
     3421 atgagataaa catgttccgg ccgcggtccg attagctttc cgtccacacg aatcgcgtca
     3481 tcccgacgta cccggaagcc catttcgctt accgtctggt cattgaccgt cacgcgaccc
     3541 tggcggatca tcgcttcagc agctcggcgg gaggagatcc ccaccccggc cagaattttt
     3601 tgcagccgct cccccgtcag agagggcgtt gcggaatcct cagcatcctt gaccatatct
     3661 taattgcctt gtagccgctg tttattccgg agaaccgata aatccggaaa tttctctcac
     3721 ccttttattc agtcaaatcc ttcagctctc ccagggtggg cagttccgaa aggtccttca
     3781 gaccgaaaac ctccagaaac tttttggtgg tgccgtagat catgggtttt cccggcacat
     3841 ctttcctccc taggatcctg atcaaccttc tttccagcag ggtccttaac ggtcggcccg
     3901 catcgacacc ccgcaatctg tccacctcgg ccttcactac cggctggcga taggcgacga
     3961 ccgctagggt ttccagggca gcggccgtga gcgccgcggg ccggccggcc ttcatcttcg
     4021 ctatccaaac cgccagatcc ggccgagtcc ggaactggta tccacccgca atctcccgga
     4081 gcaagaaacc tccccgccgc tcttcatgtg ctgccatcag ttcccgcaaa tgcaccgcca
     4141 cctcctgccg gttgatttcc ctttcctgaa ggatctcaat gatccgctcg atggtcaacg
     4201 gcgactcggc ggcaaaaatc aatccttcaa taatcgcctt gttttcattc atgttcttct
     4261 tcaacagccg gaaacacgcg gattactccg aacggtcccg cctgaaagat tcggatcagc
     4321 cgctgccgga caagttcgag caaagcgata aaggtatgaa tgatcaggtg ccgatccgtt
     4381 cgttcctcaa gtaattccgc aaaggtgagg gaccgatggc actgtaatcg ctccataatc
     4441 gtgttgatcc ggtccgctag ggaagtgcgg tccaggtcta tttccatccg ttccggtgag
     4501 gaaagcttgc caagtacctg gcgaaaggca tccactagtt cgaacagacc gatttccacc
     4561 catggcggct ccacctgcgg cagcgcttcg gtcacggccg cactccctcg ggcgaataca
     4621 tccctgtcca gcaattcccg ggcctggagg gactgggagg cgttcttgaa ggactggtat
     4681 tccagaagtt gctccaccag ttcccggcgc ggatcaagct cttcctcccc ctcttccgtc
     4741 gtttccggaa ggagcatttt cgacttgata tgaaccagtg tcgccgccag gaccaggtat
     4801 tcaccggcta gatctaaatt cagcgctttc agcgcctcaa tgtactcgag gtactgccga
     4861 gtaatcagag cgatgggtat gtcgtatata tcaagctcgt ttttcttgat cagatggagg
     4921 agaaggtcca ggggaccctc gaagatatcg agcttgacct cataatccat agctgatctt
     4981 cattgccgcc cggacctctt ccagcgtccg ctccgccacc gaccgggcac ggagatctcc
     5041 ggcggcgaca atatcccgga cctcctggag gtgatcccga tagtatgcct ggcgctcgtg
     5101 caaggggagc atagccgcac cgatgttttt cccgaggatt ttcttgcaat ccgtgcagcc
     5161 gatgccagcc tgccggcatt ccgtctcgat ctcccgggtc ttttcccaat ccgtgtagag
     5221 ctgatgaaag gtgaagacgt tgcacaactc gggccgaccg ggatcgcggc ggcgctgacg
     5281 ctggggatcg gtgaacattg aggcgacctt attttgcagc gcctcgcccg tatcgctcat
     5341 ataaatcgag ttttcatagg acttgctcat cttccgaccg tcgatcccca gaagcttggg
     5401 aacctgggtc aagagcggct ccggaatcgg gaagacctcc cggtacagaa aattgaaccg
     5461 ccgggcgatc tcccgagtca gctcgatgtg gggcagttga tcaaccccca cggggacgcc
     5521 gtaggctttg tacatgatga tatccgccgc ctggagaacc ggatagccaa gaaagccgaa
     5581 ggtggaaaga tccttgctgc tcagttcctc cttcatctcc ttgtaggtgg ggtttcgctc
     5641 cagccagggc agcggtgtga tcatggacag gaggagccac agctccgcat gctccttgat
     5701 ctgggactgg acaaagagtg tgctccggtc cgggtcgaga ccggcgctca gccagtcgat
     5761 gaccatatcc attatattct cggctatttc gctcgtatcg gcataatcgc tcgtcagggc
     5821 atgccagtcg gcaacgaaat agaaacactc ataagctccg tcgttctgca gctccaccca
     5881 gttcttgagg gcgccgtgat aatttcccag gtgcagtttg cccgtggacc tcatgccgct
     5941 caaaattctc tttttcgtca cttaacctcc gctaatccgc catccgtgat gcccatacgc
     6001 ctgggaaagc aatggcatcg cggcggcgtt gtttctatgc ttctcaccac cgtcagaggt
     6061 ggccgtataa cagaaaggcc ccggactgtc aaactcggcg ggaatattac cgctattgat
     6121 cgctgcgttt caggatgaaa aacatcctcc ccttttcctt cattgccccg gcggttcttt
     6181 ccgccaccgg tccggtgccg caaaagaggt ccacccgccc cggacccttg atggccgccc
     6241 cggtgtcgtg attcaggacc aagcgggaaa agggctgcca ggacaggatc ctgccgtcat
     6301 cggcaaatac cggtttgcgg gtctcgatca tagccaaggc ccccttggga tatagatcga
     6361 ggtccgtggc aatggacctt tccggaacta gggtgagccc cagggccccc agagggcctt
     6421 ccggaacgat gcgaaaaaag atatagcgtt gattgtagtt cataagttcc tgccgttcct
     6481 cggggcgttc gcggaggacc cgtttcatct gttcataaga cctttccccc tccgccaaaa
     6541 ggccccggga tgccatcatc cccgtcaggc cccgaaacgg ccggccgttg gactgggcat
     6601 aattcaccgc tatctggcgg ccatccgcca gacggatctt cccggagccc tggatatgaa
     6661 gcgaaaacaa atccaccgga tcatcaaccc aggcaatttc cagaccccgt cccgccagga
     6721 cgccttggga atcgatctgc cggcggctga aatggggaag cacttcgccc ttgtccaatc
     6781 tcccgatcaa tcggccctgg ccccatgtcc cctcgaagcg gtcgaggcgt attaccacgg
     6841 tttcttccgg gattcgatag aggggataac ggcaacgctc cgtccttgtg aggctgccgt
     6901 tgagaattgg ttcgtaatag cctgtaaata cagtatcttc gtcagccgcg acgatttcga
     6961 attccgcccg aatgaccctt tcctttgcca ccgccgacaa atcgcttttc atgatcttta
     7021 gaaaactctc caaaccggag atcatctcta ccgccgagta gcagcggtcc cggaggcagt
     7081 gcgtttcctc ggcgccccgg ctccggtagt attggatgct caccgcgatg gccttttcca
     7141 gggaggggag atcgagatca tcgaatggca ctgtcccttc ctgaacctga ctagaagggg
     7201 gaatgcgggg cgaatcatcc cgggacagat cggaaaccgg tctacaggaa gcgatgaaca
     7261 gcagcgtagg caggaggagc aacagtaggt ggaaacaggt atgaggtaag cgcttcataa
     7321 ttcgatgatc atcgccactt catcgcagta gtcgggatat tttggacaac ggaaacagtc
     7381 cgtccagatt ttctccgaca gagaggagcg gttcacggcg tgaaaaccga cccgttgaaa
     7441 gaattccctt ttataagtga gggtgaagat gcggaatagt tccaaggtga tggcctcgga
     7501 tatgcaggcc tccaccagga tcctgccgat gccccgccgt cggaacgtct catccacgta
     7561 cagggagcgc acctcggcca ggttttccca gatgatgctc atggcgcaga taccggtgat
     7621 gagcccttcc tcatcatcat aatagacaaa gaagtcccta aggctgccgt aaatgtccat
     7681 cagagaccgg ggcaggatct cctccttgct tgccgagaca ttgatcatct tgtgaatagt
     7741 cttgacatcg ccgatccgcg ctttcctcaa catcactctc tccttccggc cctggccagc
     7801 atttcccggg catgtttttt cgtcgtcacg gttatgtcca gaccgctgag catgcgggcg
     7861 atctcctcga cccgtgcctc ttcacccagt tcccggacct gggtctccgt ccgattctcg
     7921 cccacattct tggttaccag gtagtgccgg tctccgtaac aggcaatctg gggcaaatgg
     7981 gtgatgcaaa gcacctggtg acgctctgcc accgcccgga tctttttccc gacgatttcc
     8041 gccgtggctc cgccgatacc gctgtccacc tcgtcaaaga ccatcgtccc cacggaagcc
     8101 actcgggcca ggacattttt catggcgagg accaaccgcg acagttcgcc tcccgaggct
     8161 acccgggaaa ggggtttggg tacttccccg ggatttgccg aaagatagaa ctcgaggcgg
     8221 tcggtccccc ggggggtgaa ggtatcctcc ccattgccgt tatcctggaa gaccaccgag
     8281 aacaccgccg cctccatcct cagggcatgg atctcacgtt cgatgtcctt ctgcaatagc
     8341 ccagcggcta cctgccgggc cgccgacaac tcgccggcct tagcttccag ctcccttcgg
     8401 acggtctgga gggatgaatc tgtctcctcg agttccctgc ccagggaagc gatcccgtcc
     8461 agttcccgct ccaaagcatc tttcttttcc aacaccgccg ccaaggtggc cccgtgcttt
     8521 ctcttcagtt tgttgatcag ttcgatccgt gtttcgatct gttccaaccg ctggtcatca
     8581 ccggccaggc cctgggcata attccgaagt gtataggccg catcctccaa atgatagtaa
     8641 atgtcgtcca gttgcgcttc cgtgaccgta aggcgcggat cgatcttttt gatctccccg
     8701 acggcatttc tgacgattct tagttctttt agcagggaat gttcccggcc atagagaatt
     8761 tcgtgcgccg tcaccgcata atccgtaagt ttccggatat tggtgaggat ctgcttctct
     8821 tccgccagat ggccatcctc tccggtctcg agggctgcgg cttcgatctc agcatgctga
     8881 aaacggagga actcttcccg ctcttcccgt tgtcgctgcc ggatctccaa ggttcgccgc
     8941 cgctctttca gaacctgata tcggttatag atgacttcat agtcaccccg caatccctgg
     9001 agaccgccga aggcatcgag gatatcgaga tgatggtcct ccttgagcag gagttggtgc
     9061 tcatgttggc cgcagatatt gagaagcgat tcccccagtt cggcgagcat ggccagactg
     9121 gcaagatttc cgttgatgta aacccggttt ttcccccccc gggagataac ccgccgcacc
     9181 accatttcgt ctccagcctc cagtccccag cccgccaacc gggcccggat tccgtgctga
     9241 tccgaaacca ggaagcgggc ttccaccgtt gccgtatcgg cggtggaacg gatcatatcc
     9301 acggaagccc ggtctcccag taggagactc accgctccca tgatgatgga tttaccggcc
     9361 cccgtttctc cagtaaggat attcatgccg ttgctgaacg ttatgtccag agcctcgata
     9421 atggcgaaat ttctgatgct cagttcggct aacatcttcc tcgttaagca gccctcgttt
     9481 ctaaagatca gcgtcttttc atcctgggcg ctgtatccca gccgtcaacc cgcgccaacc
     9541 gagcttggag cgaaggattt ccatataatc gcgatggggt gagacgacaa gaagcgttac
     9601 atactcggat ttacggactg tcatgacatc tcccgatttc agggtatgaa agatctgacc
     9661 gtcaagcgtt accgtagccc cctgctcttt ggaccagagc gccacccgga tgttgacgtc
     9721 tccgggaaga atgaccggtc ggttcgtcaa ggtgtggggg cagatgggat tgataattat
     9781 ggaatcatgc tgaggaaata cgatgggtcc cccggcggaa agggaatagg ccgtggatcc
     9841 cgtgggggtg gagacgatta atccgtctgc cttgaaggtg ttcagataac agccgtccac
     9901 cgccgtttcc atttccacga tccgggacag attaccccga ttgatcacta catcgttgag
     9961 gaccgtacgt ttcttgacca cttcgccgcc gctttctatg gtgatatcga gcatcatcct
    10021 tttttccgta cgaaatccct ggcagcggaa gatttcgaag gcatcaatca tttcgttgag
    10081 attgacttcc gtcagataac cgaagccgcc cagattgatc cccacaatgg gtacggaaaa
    10141 ttcccgcacc gccctggctg tcctcaacat ggtgccgtcg ccgccgaaga cggcgatgat
    10201 atccgcccgg acagcgagtt gccgccggtc atatccggga ccgtcgccga tggacgcgga
    10261 gatgcccttg tccagataga cctcgattcc gtgctcgagg caccagtctc tcaatttttg
    10321 cgtgacttct ccggctttat ccttcgccgt attggcaacg atccctactt tacgcacctt
    10381 cttgtccacc tcgtaccagg aagtccatag ctattggctc cccgttcatc ccaccggacg
    10441 caccggccac accaatgcgc cgcttctcat taaaactggc ttctcattga ttacgaagca
    10501 gtcttcccgg catttcggcc attatctgtt cacaatcccg ggcaatagca ttcacgactt
    10561 cgccggcggc gggaagatct ctgatcgccc cggcggcctg accgatctga agttctcctt
    10621 ccaccaggtc tccatcgaac atgcccattt tcgttcgttt ttccccgatg atccggagca
    10681 gttcctcctc tccagccccc ctcttttccg cttccacaac cctctcatac caggcgtttt
    10741 taattacccg ggccggaatc aatttacgga gaatgagaaa ggtatcggta ggagaggcct
    10801 ccagcatcat tcgcttgaaa ttttcatggg cgctggactc gcgggtcgca gcaaagcggg
    10861 tgccaatctg gacccccgac gcccccatgg cgagcgcggc ggccatcccc cggccatcga
    10921 atataccccc ggcggcaatc acgggaatct tcactgcatc gacgctctgg ggtacaagga
    10981 ccatggtggt caattcatcc cgaccgttat ggcctccggc ctcaaatccc tccaccacca
    11041 cggcatctac accggcctct tcacacttga cggccagttc cggggtcgcc gttacatgcg
    11101 ccaccttgat cccacgttct ttgaagcggg agatgtattt acgggggctg ccgccggagg
    11161 tgaagaccac ccggactttt tcttccagga cgatctgggc catctcttcg gcatactgaa
    11221 aaaatatcgg gagattcacc ccccagaccc cctcgatgcc ttcctttttg atcttgcgga
    11281 tgtgctcgcg gagcagatcc ggggtcatgg agccggcgcc gataagaccc agggcacctg
    11341 ctttagccac ggctgcggcc agacgccagc cgctgaccca gatcatgccg ccctggacga
    11401 taggatagcg gatcccgaag atttccgtaa ctctagtagc gatcattttt tcttttccta
    11461 tagatttatt gggttcatat ctttagaaat tacattaaga aataggtccc gctggccacg
    11521 aaattttccg ggcctataat cacacaccac cacaccataa aatgtaatga tacgacctgc
    11581 caccaccacc aaagatccga gtttgccagc ctgtatttgc agacgatcat gttacaattt
    11641 tttttaaggt ggcattatcc gagtgcctgg cagtattctt cccggacata gtccggcagg
    11701 gaattcccca tggcctccga gaggatatcc gccgccccgc ccatggcggc cagggtccgc
    11761 accatcagca cctccttgaa ggtgcggggg ggataaaccc tccgcacggt gcttctttcc
    11821 cggtcaaaga actggagata accccgtcgc accgtccgtc cgtagacatt gatcgtgacc
    11881 gcgatatctc cggaggcgtt ctccatttga tgaataccgc cgtcaagagg aaagacggtg
    11941 gttatctctc caggtttcaa caccttttcc tctgtcgctt cgagctccgc atgacccgcc
    12001 acggcgccgt catcgagacg gagatactta acctccctga ttttattgat ataacttccc
    12061 actatccccc aggaaccgtg atcatggatc gtatccacgg aataggactc ccagatgtag
    12121 gccaggataa gaaaagactg gtcgggactg cgatagaggg tgatttcgtt cggccagagg
    12181 gtaggcggtt gctgtttgaa ccaggtcctc tctgctagga cctcccggat gaagtcgcgc
    12241 caccagagat ccgaggaaag gagttcctga accagtggga ttcccgcagc gacggcgttt
    12301 tcggcatcct cttttccggc agccgccgct ttgaaccgtc ggcaaaaact ttcaaaatcc
    12361 ttatgcactt attcaactct ccttatcaaa ggcgtatcat tctccctccg ctcgttgaag
    12421 tcaggggagg aggaaatctc aacgcgtttt tttataataa agcctcagat tcccccttac
    12481 cctcttgtga ccctgtcaca gattacggtc accgtggcct tggcagcctt tagtgaatgt
    12541 atgcccttcc ccaccattca tggctccttg acgccgtatc gccggatatg ctgccaggaa
    12601 cggtagtcca catgatcggg gtcggccacc agcacttccc gttcccggag atatttcttc
    12661 atcatgccct tttgttggta gagcttccgg tcggcaccga cgggacagac cttgatgcag
    12721 atcccgcagg gatatgaccg cctgctggtg agttcttctg ccatttccag gcacgccgtt
    12781 ttgtcgtagt cccctggaat acggtcatca cggacccgca gggcctcttt ggggcaacac
    12841 ttcacgcagg cctcgcattt gatgcacagt tctttctcca ccagggggtc gggaggcagg
    12901 gcggcggcgg tgaaaataga aacgagacga acccggggtc cgaattccgg cgtcaggagg
    12961 cagtggttcc ggccgatggt tcccagaccc gcgtatttgg ccgcctggac atgaccgaag
    13021 gcggctccgt tccaacgacg caggtcctta aggctgctgt aaccgtcacg gctgaagaaa
    13081 gttgcgacat gaccaaggcg attcagatgg cgggtgacat cataagctag cgcgtcaagc
    13141 tttctgttga cggttttgta caattccata tgcatgacag atggtgttgt ttccacaata
    13201 ggcagcggca tgcccagccc gaggacgacc acggtccgcg ccggcgacca gattccctga
    13261 ggacggaatt ccagagggat aatcccttcc tcaatccatc tttccaccgg ggcaaaaccg
    13321 acgatgtccg cccccgcttc ctgacaaaaa tatttaaggt tatatgataa ttcctctttt
    13381 ttcatggcgg cattttaaac tatttctccc cccatgtaaa tacgagaaac caccgggaga
    13441 atcaatttgt gtgctcttgg aaaccaacgc ggggaatgac cagaagtgga aatgtccgaa
    13501 gattattctc ccctgtaggc gtataaaaaa acccgc
//
LOCUS       DAOSMF010000042        13305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_5416_length_13305_cov_18.459774, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000042 DAOSMF010000000
VERSION     DAOSMF010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 13305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13305
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5416_length_13305_cov_18.459774"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /gene="pilO"
                     /locus_tag="PLT63_05900"
     CDS             <1..543
                     /gene="pilO"
                     /locus_tag="PLT63_05900"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HOD98290.1"
                     /translation="IAAQKDKYLKEVAVLKEAFVIALTKLPDQREIPGLFQAVSVAGR
                     EAGIDFLLFEPKPPEKPAAPVGKGAEVRANLKPSDQRAEQKPGTPSKGGPTPAGAADR
                     FYEEIPVKVTINGGFVNTVFFFDKVSRLPRIINIEDVSIGDAKDVPGRGRLANTSCVI
                     KTYMFTEKMDKGGRGDDKKK"
     gene            527..1084
                     /locus_tag="PLT63_05905"
     CDS             527..1084
                     /locus_tag="PLT63_05905"
                     /inference="COORDINATES: protein motif:HMM:NF016254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HOD98291.1"
                     /translation="MTRKSEMIAVLIMAGFIFSAGPAGAQTGGATASPPRGAAYPPDA
                     RPPVAAESSLIFRYDPKGKPDPFKPFLEAELALRKLKELEKRRLQKKLPLSPLQRAPI
                     DQFRLVGVGGVPKSRTALVQDQAGKYYTLNIGVPIGTKNGRVVKILADRVIIDEPVAG
                     VDKKGKRGVKQIEMKLFRINDEGKP"
     gene            1081..2763
                     /locus_tag="PLT63_05910"
     CDS             1081..2763
                     /locus_tag="PLT63_05910"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secretin and TonB N-terminal domain-containing
                     protein"
                     /protein_id="HOD98292.1"
                     /translation="MKEYKLLVGFALLCLLWVLTAAGSAGGQATGGGAPAVEAGYLEE
                     IRFEPLPGKERVTVVATRQAGVQIENLAGKGVVVHMEEIYVPTDLRKSLGEKRLQNVI
                     NAVPEQVQSKGRLAARIVIALREAVPHSVRQEGNAVIIDFNVAALPAAAAPAAKTTPT
                     AAAATPASKETPVATTAPETMTVNIPKMSVDFQEADIRGVLRLLAEQGKVNIVTSPEV
                     KGNVTVRMDDVSWEQVLATILEINGLRKKQSGNVISIMTADRAKKDEDDRIATLQKHQ
                     KAEEEAKKAAINKNLEMGRLKQLMIEAKILEVTEGFIRNLGVQWGAGVQLGLANNDYS
                     MGLLGGTGTLTTPITKLNTGVSLTRDALAMSLGTTLAPTLGIILGGSNAILEARINAM
                     ETNSSGRVLSAPRVLTQDTQKAVIEQGEEIPIVTPASANNPATTTYKPATLKLEVTPE
                     IKDDNHILMTIKAKNDRPNKAEKDANTGNMPVYTSSVDSKVVVMDGDTIVIGGILKTE
                     DTKKVDGVPWLSQIPVLGWLFKYEYVEKTKRELLIFLTPRIVKASDASFTRM"
     gene            2769..2939
                     /locus_tag="PLT63_05915"
     CDS             2769..2939
                     /locus_tag="PLT63_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98293.1"
                     /translation="MFHLVSPAQQAGSSHTTSSKEVPLELRVTPEIKKDNHMIIITPR
                     IIKPEEAGFANI"
     gene            complement(3191..6565)
                     /locus_tag="PLT63_05920"
     CDS             complement(3191..6565)
                     /locus_tag="PLT63_05920"
                     /inference="COORDINATES: protein motif:HMM:NF017388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HOD98294.1"
                     /translation="MKPLHPIACLLAAFFIGSWLILGHPMPASATAPAEGEEALFTNT
                     TSPDALILIDLSGSMDWNPAGGDRKYGISSCAASTASCCTSTAAEENGFCSSSKTNCD
                     TNCSRLAIAKRSLFDLLDDNDDNTINSSDEGSLGVRIGYMRFYDCNSDDTSDDYTSGC
                     IKIPGSSSSRRYIGSKYSQIYCASNSSCTITSGSSSSYCINGESALGGTPLGSALRKA
                     KVYLDAHKTADTAKDCRKKFVIFITDGADTFSCGGPGYECSDDGWNYSAQRYKQRREA
                     VARAKAAADAGYKVFVVGFGAAMPTYLQNTLNWMAYYGGTDNPDVANAGSTSGYTSVA
                     VGSCTADADAVTATCYDSGGAHNTTKFRASSNDPGYADLSGYAFLATDADTLASSLKS
                     AMKIIQQANYSFSQSSVQSSRTSDENHLYEGSFEPLDNDSFWKGHLKKFKITNTEAEA
                     AACTCYEADGTTIRPCVVGDVCGVDTSFGGNGDAGNALQTRNHYTRNIKTYKGGYLIN
                     FSRYVPLFSDCTIYPYCDGTINTGIENITITDVGVTTTAERDAIIGYIRGNGDYMPAY
                     AAEYPEKNASGQTWKLGDVFRSTPITVGTPSAFYYDARDANSEFNDHRTNHPRTSTNG
                     YRLIVAGANDGQMHAFKTSNGSEAWSFLPPNLMSKLKNMTHTTHPTALGHQYFVDGPV
                     TVADVWCVTPNASCGTGAAKADNSWATIMVFGLGRGATNHSWSSSQYCDSGISATYSA
                     SYPYYCGYHALNLTSSLAPTYLWRMSPNSTQAPYFGDSWSKLMTGRVRINDSSGNIIE
                     KWVGFFGAGYNASDCSGGGTCDPRGKGVFIVDLYDGSILWSYTLGSTTTSTTNTGMKW
                     SVPAQAAIVDTDNDGFIDLAYIGDMGGKIWRLKFCLSTDSYCSHTAGAGITKTWSGGM
                     FFDSPSGNIRPIFTSPAVSKDAYGNLWVYWGTGDKVDPTAANAQEHFYGVKDNDRTTT
                     WSGSDIDNLASANQTYDTSATDKVGWRIQLTGGGEKILADPTVFGGVVYFTTFTPASG
                     NNPCEQGGTAGLYAVNYNTGAGALSEGARSMTIGSGIPSAPVISLKPGSSATPDLYVT
                     TSGGGGTSASTQRVDINPVGVSNRTNMLYWRDRRLQ"
     gene            complement(6576..7199)
                     /locus_tag="PLT63_05925"
     CDS             complement(6576..7199)
                     /locus_tag="PLT63_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98295.1"
                     /translation="MIHKGNRRIHLIPSPGKRKGTPDGQNNGRAQAGRGSPPPAGRRL
                     RIEGSLRNRGKRGCEGGFALVAAILACVILIALALLVINLSSQDLKSSAKNVGDKKAA
                     AAAETGIHKMMQNFDPQNLSSSAQTNIQVDPNNDPNSVYTIGTPTRPTTGPTFLPLTG
                     FSIGGGQSWGQARYVVDVTGRNTAYGTSVTIGTGVGFGPIEISTMSR"
     gene            complement(7213..7953)
                     /locus_tag="PLT63_05930"
     CDS             complement(7213..7953)
                     /locus_tag="PLT63_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOD98296.1"
                     /translation="MKNIRGVTLVELLVSMFVAMILLGSVYMAVNVAQRSSAGIERKV
                     TAQQDVKPALDLMAMEIGMAGYNPNYASGLWLNPATCATAANQNYKGIQEATTNAITI
                     EMDITDSACTATDGDGAISRCNEIIRYEYLSGATDQRITREEISCVSGARTTGNAQPF
                     LGDAAASGNPRTVLVINNTAGIPVFRYYDGTGTEIAAGSLPGAIPDIRRVEITLAVET
                     AEIDPNTGQRRRLIYTTSVIPRNNIIIP"
     gene            complement(7950..8399)
                     /locus_tag="PLT63_05935"
     CDS             complement(7950..8399)
                     /locus_tag="PLT63_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOD98297.1"
                     /translation="MINVRRVLSDRRGFSILEVVIAIFLTTVGVLAIMSLQPAAWQTA
                     FRSDYMGRAAGILHEELQTRESFIMNCCNTVTAGTTEATVYAGGGTSAQAAGDARFTV
                     TTTIASVDTNVWRVTVRVAWHGHTGISESLVVTRQQAFSYPAGCSCT"
     gene            complement(8413..8892)
                     /locus_tag="PLT63_05940"
     CDS             complement(8413..8892)
                     /locus_tag="PLT63_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HOD98298.1"
                     /translation="MYRKRLNGQGGFSLIELIIVIAVLGILGAIASYSYMGYVNNSNL
                     RSAARDMAADMANTKQKAVSEGLTYRTIISVDANTYTIERGDAAGTTFTVVETKSPTA
                     FGTGLTISSTNYTSSQITYQPRGTISAGSVVLINSRDSTATITSNITGRTHVSFTIK"
     gene            9413..10933
                     /locus_tag="PLT63_05945"
     CDS             9413..10933
                     /locus_tag="PLT63_05945"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HOD98299.1"
                     /translation="MKPKNEQVTPRIFFSSGYPVPDDLLHNLSYEFLRRELWTPLGKK
                     EGRIQVAVDEPENLLKKDMIENLLRTKTVDYLGATREDILKFIDHFYGVEEGGRDNGV
                     VVSESDSKIVRLVNEMINEACHRRASDIHVEPDGKDRQVYIRFRIDGECIGFKAHPYD
                     YRAAIVSRIKIMANLDITERRLPQDGKIQYKRPNGEEIELRVATIPTHGYVEDVVMRI
                     LTRGKIMTMGELQMEPDTRERFEGLLKKPYGLILLVGPTGSGKTTTAHAALHILNRPG
                     VKIWTVEDPVEITQKGIRQVQVHHKIGLDFSNAMRAFLRADPDIIMVGEMRDYETAKM
                     GVDASTTGHLVLSTLHTNNAPETIIRLLDMGIDPFAFADSLLCVMAQRLVRRLCRHCR
                     APYHPDPEEFADLVRNYGEKEFQRFPFAYDETTTLYRAGGCPECHDTGYRGMMGLYEL
                     LIATDPIKQMIIGRKSIAAVRKTAMGEGMRTLMQSGILKVISGDTDFVEVLSVCMR"
     gene            11059..11685
                     /locus_tag="PLT63_05950"
     CDS             11059..11685
                     /locus_tag="PLT63_05950"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD98300.1"
                     /translation="MDFYQQARNLLDEGNYTRSLHLAQERLRAAPEDVPATLVLCRSW
                     LGLGRLEEAAAAFAAIEPMNEEIARLLKLLGDAFQGRGDHGRAVKCYQRSMALLPGVE
                     ELREVQNSLAELMEDDEEPTAGPAGGEGFQTLTMAELHIRQGHYEGAREILTAILLRE
                     PEHREARRRLTEVQEQINKKNCDLPIDKKTTVVHELSRWLIKLGGKGQ"
     gene            11682..12776
                     /locus_tag="PLT63_05955"
     CDS             11682..12776
                     /locus_tag="PLT63_05955"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOD98301.1"
                     /translation="MTDDAADIDRKRIERVYERIDPKEFDAILFFDMNNIRYLTGFTG
                     SDGVLLLQPDRVMLLVDGRYVTQAAEQSPHCDVVQYRDKVSGIATALAERGAGRVGFE
                     AFALTVGLYEKIRERIRTTRLAPLDGELSCLRSRKDAGEASLLRRAVDIATEALTDVL
                     PLLRPGRTEREIALELDYRMRALGSEENSFPTIVASGPNAALPHARPGDRKLAPDDPV
                     IIDFGAVYRGYHSDETCTLVVGRPDAEFRQVYDIVKEAHDRAIAAVRTGVSGREIDGI
                     ARRHIEEQGYGPQFSHGTGHGVGLDVHEDPRLSPLSDQVLAADMVVTVEPGIYLPGRW
                     GVRIEDMVLVGEDGCEVLTRMPKELKNIDL"
     gene            12804..13202
                     /gene="gcvH"
                     /locus_tag="PLT63_05960"
     CDS             12804..13202
                     /gene="gcvH"
                     /locus_tag="PLT63_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOD98302.1"
                     /translation="MKVFPDDLLYSREHVWVRVEGDLATIGITDYAQEKLGEVLAVDL
                     PEVDTEVERDEPFGAVESSKGAVELISPLSGNVLNVNEDLIDDIGIVNSDPHDTGWMI
                     VVEMHDLDELDDLLDARGYHDFILQETEGD"
BASE COUNT         2699 a         3675 c         4273 g         2658 t
ORIGIN      
        1 attgccgcgc aaaaagataa atatctcaaa gaagtggcag tgttgaagga ggccttcgtc
       61 atcgccctca ccaagcttcc ggaccagcgg gagataccgg gactcttcca ggctgtttcc
      121 gtggccgggc gggaggcagg aatcgatttc ctgctcttcg aaccgaaacc gcccgagaaa
      181 ccggcggcgc cggtgggaaa gggcgcggag gtcagggcaa atctgaaacc atcggaccag
      241 cgggccgagc agaaaccggg aacgccgtcc aagggagggc ccacgcccgc cggagccgcg
      301 gatcggtttt atgaagaaat acccgtgaaa gtcactatca acggcggatt cgtcaacacg
      361 gtcttctttt tcgacaaggt gtccaggttg ccccgaatca tcaatatcga agatgtctcc
      421 ataggcgacg ccaaggatgt ccccggccgg gggcgcctgg ccaacacgtc ttgcgtgatc
      481 aaaacctaca tgtttaccga aaaaatggat aaaggcggca ggggtgatga caagaaaaag
      541 tgaaatgatt gcggtgctca tcatggccgg tttcattttt tccgccgggc ccgcgggagc
      601 ccagacggga ggcgccaccg cgtcccctcc ccgaggcgcc gcctatcccc ccgatgccag
      661 gccgcccgtt gcggcggagt catccctgat ctttcggtac gatccgaagg gtaaacccga
      721 tcccttcaag cctttcctgg aggcggaact ggccttgaga aaattgaagg agctggaaaa
      781 gcggcgtctc cagaagaaac ttcccctgtc accgttgcag cgggcgccca ttgaccagtt
      841 tcgtctggtg ggcgtcgggg gcgtgccaaa gagtcggacc gccctggtcc aggatcaggc
      901 cgggaaatat tataccctga acatcggcgt acccatcggg acaaaaaacg gtcgggtggt
      961 gaagatccta gcggaccggg tgataatcga cgaacccgtg gccggtgtgg ataagaaggg
     1021 aaagagggga gtaaaacaga tcgaaatgaa gctgttcagg attaatgatg aggggaaacc
     1081 atgaaagaat ataaattatt ggtgggtttt gcccttttat gtctgttgtg ggtgttgacg
     1141 gcggcgggaa gcgccggggg ccaggcgacg ggaggcggtg ctcccgcggt agaggcggga
     1201 tatctggagg agatacgctt cgaaccgctg ccggggaaag aaagggtgac cgtagtcgcc
     1261 acgcgccagg ccggggtgca gatcgagaat ctggccggga agggcgtcgt ggtgcatatg
     1321 gaggagattt atgtcccgac ggatttaaga aaatccttgg gagagaagcg tttgcagaac
     1381 gtcatcaatg ccgttccgga acaggtgcag agtaagggcc ggctggcggc taggatcgtc
     1441 atcgccctgc gggaggcagt tccccacagc gtccgccagg aaggtaacgc cgtgatcatc
     1501 gatttcaacg tggcggccct gcccgccgca gccgcgccag cggccaaaac gaccccgacg
     1561 gccgcggccg cgacgccggc gagtaaggaa accccggttg caacgaccgc cccggagacg
     1621 atgaccgtga acatcccgaa gatgtccgta gattttcagg aagcggatat ccgcggcgtt
     1681 ctgcgtctcc tcgcggagca gggaaaggtc aacatcgtca ccagtcccga agtgaagggg
     1741 aacgtgacgg tgcggatgga tgacgtttcc tgggaacagg tcctcgccac gatcctcgag
     1801 atcaacgggc tgcgcaaaaa gcaaagtggc aacgtcatca gcatcatgac cgccgaccgg
     1861 gcgaaaaagg acgaagacga ccgcatcgcc actctccaga agcaccagaa ggccgaggaa
     1921 gaggctaaaa aggcggcgat caacaagaac ctggaaatgg gccggttgaa acagttgatg
     1981 atcgaggcga agatcctgga ggttacggag ggattcatcc gcaatctcgg cgtccagtgg
     2041 ggggccgggg ttcagctcgg tttggccaat aacgattatt ccatgggtct tttgggcggc
     2101 acggggacgc tcaccacccc catcacgaaa ctcaacaccg gcgtttccct gacccgcgac
     2161 gccttggcca tgagcctggg gacgaccctg gcgcccaccc tggggatcat cctcggagga
     2221 tccaacgcca tcctggaggc ccggatcaac gccatggaga ccaattcctc cggccgggtc
     2281 ctctccgctc cccgggtcct cacccaggac acccagaagg cggtcatcga acaaggcgag
     2341 gaaatcccca tcgttacccc cgcttcggcc aacaaccccg cgacgaccac ctacaaaccg
     2401 gcgaccctca aattggaggt aactcccgag atcaaggatg acaatcacat cctcatgacc
     2461 atcaaggcaa aaaacgaccg gcccaacaag gcggaaaagg acgccaacac ggggaacatg
     2521 ccggtttata cgagcagcgt cgattccaag gtggtggtca tggacggcga taccatcgtc
     2581 atcggcggca tcctgaaaac ggaagacacc aagaaagtcg acggcgtccc ctggctttcc
     2641 cagattcccg tcttaggctg gcttttcaag tacgagtatg tggaaaaaac gaaacgggaa
     2701 ctgcttatct ttcttacccc gcgcatcgtc aaggccagcg atgcgagctt tacgaggatg
     2761 taagatggat gtttcacctt gttagtccag cacaacaagc tggatcttct cacacaacaa
     2821 gcagtaaaga agtgccctta gaattgaggg ttacaccgga aatcaaaaaa gataatcata
     2881 tgatcattat cacccctcgg atcattaagc ctgaagaggc gggctttgcg aatatctgag
     2941 gatttaaccc gtacaaaatg ccgccgccgt ggtggccgtt gccacggacc ggggcgcata
     3001 cgaaaaatgg gtttttgaga agatcggtaa ggcatatctc cccatgcttc cggtgacgga
     3061 ggattttata tcgagtatgt cggagctccg acatactcga aggtattgat attattggct
     3121 aataagcgac tcgaaaaaag ttttaccttt tcgggaggcc tcattaaatc ctgcccgcat
     3181 tcttcattgt ttactgaaga cgccggtccc gccagtagag catattggtc cggttggaga
     3241 cgccgacggg attgatgtcc acccgctggg tgctcgccga tgtaccgccg ccgccgctgg
     3301 tcgtcacgta gagatcgggg gtggcgctgc tgcccggttt cagggagatg acgggcgccg
     3361 aggggatgcc gctgccgatg gtcatgctcc gggctccttc cgagagggcg ccggccccgg
     3421 tgttgtagtt cacggcgtac agacctgccg ttccgccctg ctcacagggg ttgttccccg
     3481 aagcgggggt gaaggtggtg aaatagacca cgccgccgaa gaccgtggga tcagcgagga
     3541 tcttctcccc gccgccggtg agctggatcc gccagcccac cttgtccgtg gcggaggtgt
     3601 cgtaggtctg attggctgag gcgagattgt cgatgtcgct gcccgaccag gtcgtggtcc
     3661 tatcgttgtc cttgacgcca tagaagtgtt cctgggcgtt ggcggccgtg ggatccacct
     3721 tgtctcccgt accccaatag acccagagat tgccgtaggc gtccttggaa acggcggggc
     3781 tggtgaagat ggggcggatg tttcccgaag gggaatcgaa gaacatgccg ccgctccacg
     3841 tcttggtaat ccccgcgccg gcggtgtggg agcagtagga atccgtggag agacagaact
     3901 ttaaacgcca aatcttccct cccatgtcgc cgatataggc cagatcgatg aagccgtcgt
     3961 tgtccgtgtc cacgatggcc gcctgggcgg ggacgctcca tttcatcccc gtgttcgtgg
     4021 tgctagtagt ggtggagccc aaggtatagc tccagaggat cgatccgtcg tagagatcca
     4081 cgatgaagac gcccttgccc cgggggtcgc aggtcccgcc gccggagcag tcggaggcat
     4141 tgtagccggc gccgaaaaag cctacccatt tttcgatgat gttgccgctg ctgtcgttga
     4201 tcctcaccct tcccgtcatc agcttgctcc aggaatctcc gaagtaggga gcctgggtgg
     4261 agttggggga catccgccag aggtaggtgg gggccaggga actcgtgagg ttcagggcgt
     4321 ggtagccgca gtaatagggg tagcttgccg agtaggtggc gctgatcccg gagtcgcaat
     4381 actgggacga actccaggag tggttcgtcg ccccgcgtcc caggccgaag accatgatgg
     4441 tagcccagga gttgtccgcc ttggcggcgc cggtgccgca actggcgttg ggcgtcacgc
     4501 accagacgtc ggccaccgtc accgggccgt caacgaagta ctggtggccc agggcggtgg
     4561 ggtgcgtcgt atgggtcatg ttcttcagct tcgacatcag gttggggggg aggaaactcc
     4621 aggcctcgga gccgttgctg gtcttgaagg cgtgcatctg gccgtcgttg gccccggcga
     4681 cgatgaggcg gtagccgttg gtagaggtcc gcgggtgatt ggtccggtgg tcgttgaact
     4741 cgctgttggc gtcccgggcg tcgtaataga aggccgaggg ggtgcccacg gtgatggggg
     4801 tggagcggaa gacatcgccc agcttccagg tctggccgct ggcgttcttc tccgggtact
     4861 cggcggcata ggcgggcatg tagtcaccat tgccccggat gtagccgatg atggcatcac
     4921 gctccgcggt ggtggtgacc ccgacgtccg tgatcgtgat attttcgatt cccgtgttga
     4981 tggtgccgtc gcaatagggg tagatggtgc agtcgctgaa cagtggcaca tatcgggaga
     5041 agttgatgag ataccccccc ttgtaggtct tgatgttccg ggtgtagtgg ttccgggtct
     5101 gcaaggcgtt gccggcgtcc ccattcccgc cgaagctggt gtccacgccg cagacgtcgc
     5161 cgacgacgca gggacggatc gtcgtcccgt cggcctcgta gcaggtgcaa gcggctgctt
     5221 ccgcctcggt gttggtgatc ttgaatttct tcagatggcc cttccagaag gaatcgttat
     5281 ccaggggttc gaaggagcct tcatagaggt ggttctcgtc ggaggtccgg gaggactgga
     5341 cggaggactg ggagaaggag tagttggcct gctggatgat cttcatcgcc gactttaggg
     5401 acgaggcgag ggtgtcggca tcggtggcga ggaaggcgta cccggaaagg tcggcatagc
     5461 cgggatcgtt ggaactggcc cggaatttgg ttgtattatg ggcgccgccg ctgtcgtaac
     5521 aggtggccgt aacggcgtca gcgtcggcgg tgcatgaacc caccgctacg gaggtgtagc
     5581 ccgaggtgct cccggcgttg gccacatcgg gattgtccgt cccaccgtag taggccatcc
     5641 agttgagggt gttctgcagg taggtgggca tggcggcgcc gaagccgaca acgaagacct
     5701 tatagcccgc gtcggcggcg gccttcgccc gggccacggc ctcccggcgt tgtttgtagc
     5761 gctgggcgct gtaattccat ccgtcatcgg aacattcgta acccggaccg ccgcaggaga
     5821 aggtatcggc gccgtcggtg atgaagatga cgaacttctt ccggcaatcc ttggcggtgt
     5881 cggcggtctt gtgggcatcg agatagacct tggccttcct gagggccgag cccagaggcg
     5941 tgccccctaa agcggattcg ccgttgatgc agtacgagga actgcttccc gacgtgatgg
     6001 tgcagctcga gttgctggcg cagtagatct ggctgtattt ggagccgatg tagcgccgcg
     6061 acgaagagct gccggggatc ttgatgcagc cgctcgtgta gtcgtcgctc gtgtcgtcgc
     6121 tgttgcagtc gtagaaacgc atgtaaccga tgcggacccc gaggctcccc tcgtcggagg
     6181 agttgatggt gttgtcgtcg ttgtcgtcga ggaggtcgaa gagggaccgc ttggcgatgg
     6241 cgaggcgact gcagttggtg tcgcaattgg ttttagagct gctgcagaat ccgttttcct
     6301 ccgcggcagt gctggtacag catgatgcgg tactggcggc acagcttgat ataccgtatt
     6361 ttctgtcgcc tcccgcgggg ttccagtcca tgcttcccga gaggtcgatg aggatgaggg
     6421 cgtcggggga ggtggtgttg gtgaagaggg cttcttcacc ttcggcgggg gccgtggcgg
     6481 acgccggcat gggatggccc aggatcagcc agctcccgat gaaaaaggcg gccaggagac
     6541 acgcgatggg gtgcaagggt ttcataaaga cctccctatc gagacatggt ggagatttca
     6601 atgggcccga agccgacgcc cgtgccgatg gtgacgctgg tgccgtaggc ggtgttccgg
     6661 ccagtgacgt ccacgacgta gcgggcctgt ccccaggact gccccccgcc gatggagaac
     6721 cctgtcaggg ggagaaaggt cgggccggtg gtgggccggg tgggcgtacc gatggtataa
     6781 acgctgttgg ggtcgttgtt cggatccacc tggatgttgg tctgagccga ggaggataga
     6841 ttctgggggt cgaagttctg catcatcttg tggattcctg tttccgcggc ggcggcggcc
     6901 ttcttgtcgc cgacgttttt ggcgctgctc ttgaggtcct gggacgagag gttgatgacg
     6961 agcagggcca gggcgatgag gatgacgcag gcgaggatgg ccgcgacgag ggcgaagccg
     7021 ccctcacagc cccgttttcc ccggtttctt aagctgccct ctatccggag ccggcgcccg
     7081 gcaggcggcg gtgatcctcg cccggcctgc gcccggccat tgttctggcc gtcaggggtt
     7141 ccctttcgct tccctggcga cgggatgaga tgaatgcgcc tgttcccttt atggatcata
     7201 tagttctctc cttcagggga tgatgatgtt gtttctcggg ataacactgg tcgtataaat
     7261 cagtcgtcgc ctttgcccgg tgttggggtc gatctccgcc gtttccaccg ccagagtgat
     7321 ttcgacgcgg cggatgtcgg ggatggcccc cggcagactg ccggcggcga tctccgttcc
     7381 cgtgccgtcg tagtagcgga agacggggat tcccgccgtg ttgttgatga ccaggaccgt
     7441 ccggggattg ccgctggcag ccgcatcccc caggaagggc tgggcgttgc cggttgttct
     7501 cgctccagaa acacaactga tctcctctct ggtgatgcgc tggtccgttg ccccggaaag
     7561 atattcgtaa cggatgattt cgttgcagcg actaatagcg ccgtcgccgt cggttgccgt
     7621 gcaggcgctg tcggtgatgt ccatttctat cgttatggcg ttggttgttg cttcctgaat
     7681 gcccttgtaa ttctgattgg ctgcggtagc gcaagtggcc gggttgagcc ataggccact
     7741 ggcgtaattg ggattgtagc ctgccatgcc tatctccatg gccatgaggt ccagggccgg
     7801 cttgacgtcc tgctgggcgg tgacctttct ttcaatgccc gcgctggaac gttgggcgac
     7861 attgaccgcc atatagaccg atcctaggag gatcatggcc acaaacatcg ataccagcaa
     7921 ttcgaccagg gtgacgccgc ggatgttttt catgtgcatg agcaccccgc tgggtagctg
     7981 aaggcctgct ggcgggtgac gaccaggctt tccgagatcc cggtatgccc atgccaggcg
     8041 acccggaccg tgacgcgcca gacattggtg tcgacgctgg cgatggtggt cgtcacggtg
     8101 aaccgggcgt cgccggcagc ctgagccgat gtcccgccgc cggcatagac ggtggcctcg
     8161 gtcgtaccgg cggttaccgt attgcagcaa ttcatgatga aggactcccg ggtctggagc
     8221 tcttcatgga ggatgcccgc cgcccgtccc atgtagtcgg aacgaaaggc ggtctgccag
     8281 gccgccggct ggagggacat gatcgccaga acccctacgg tggtgagaaa gatggcgatc
     8341 accacttcca ggatgctgaa accccggcgg tcggacagaa cgcgccggac atttatcact
     8401 gggtgaggag tcttatttga tcgtgaaaga cacatgggtc ctccccgtga tattgctcgt
     8461 aatcgtggcc gtggagtcgc ggctgttgat caggaccacg cttcccgctg aaatggtgcc
     8521 ccggggttga tacgtgattt ggctgctcgt atagttggtg ctgctgatcg tcaggcccgt
     8581 tccgaacgcg gtgggacttt tcgtttccac aacggtaaag gtggtgccgg cggcgtcgcc
     8641 cctctcgatg gtgtatgtat tggcatcgac actgatgatt gtgcgatagg tcagcccctc
     8701 ggagacggcc ttctgtttgg tattggccat gtcggcggcc atgtcccggg cggcgctgcg
     8761 aagattgcta ttgttcacat atcccatata ggaataggag gcgatcgccc cgaggatacc
     8821 caacacggcg atgacgatga tcagctcgat aagggaaaac cctccctgtc cgttcagcct
     8881 cttcctgtac ataaccttga taacaccctc aatccgcttt tttttgccgc ttcattatag
     8941 gccagtacgt aaggtaaagc aagatattct cgactgatgg agcaatatgc gtaccacggt
     9001 tgtcctaacg gtagccgccg gattgctggc cggagatggg ggcggtaaaa actgctcttt
     9061 actacgaaat ggcgttgcgg atagcggcgg tttgccgggc cgcgcaacca tgtcgagatg
     9121 ccatgcggtg gcgccgatcc gtggagatca ccaatatggt ggtgaaatac accatgatcc
     9181 cttccggccc taatgcgcaa gattagattc tatctgatta agatattttt tttaaaccca
     9241 tttaatatcc ggcggagagg gaattatcaa ttgacaatct tcgggagtcg ataataatat
     9301 gctatgttag attatgggcg gtcagcgatt gttcgtcggg acgccacaca attccggcgg
     9361 tgacggtcgc gacacccctt gcggggaata atgcaacatg ccgcatcatg cgatgaaacc
     9421 aaaaaacgag caagtaactc cccggatttt cttcagttcc ggctacccgg tccctgatga
     9481 tctccttcat aacctcagct atgaattcct ccggcgggag ctgtggaccc ccctggggaa
     9541 aaaggaaggg cgcatccagg tcgccgttga cgagccggaa aacctcctga aaaaggacat
     9601 gatcgagaac ctgctccgga ccaagacggt ggattacttg ggagccactc gggaagatat
     9661 cctgaaattc attgatcatt tttacggtgt cgaggaagga gggcgggaca acggggttgt
     9721 cgtctccgaa tcggacagca agatcgtccg gctggtaaac gagatgatca acgaggcctg
     9781 ccaccgccgg gcttcggata tccatgtcga gccggacggt aaggaccgtc aggtttacat
     9841 ccgcttccgc atcgacgggg aatgtatcgg cttcaaggcc catccgtacg attatcgtgc
     9901 ggccatcgtt tctcggatca agatcatggc gaatctggat atcaccgaac gccgtctgcc
     9961 tcaggacggc aagattcagt acaagcgtcc caacggcgaa gagatcgaac tccgggtggc
    10021 gaccattccc acccatggtt acgtcgaaga cgtggtcatg cgcattctta cccgggggaa
    10081 gatcatgacc atgggagagc tccagatgga gccggatacc agggagaggt tcgaaggcct
    10141 cctgaagaag ccctacggtc tcatcctgct cgtcggtccc accggttcgg gaaagacgac
    10201 cacggcccac gccgccctcc atatcctgaa ccggccgggg gtcaagatat ggaccgtgga
    10261 agatccggtg gaaatcaccc agaagggaat ccgccaggtc caggtgcacc acaagatcgg
    10321 cctcgacttc agcaacgcca tgcgggcctt cctccgagcc gacccggaca tcatcatggt
    10381 gggggagatg cgggactacg aaacggccaa gatgggcgtc gatgcctcca cgaccggcca
    10441 tctggtcctc tccaccctgc acacgaacaa cgccccggaa accatcatcc gcctcctcga
    10501 tatgggcatc gaccccttcg ccttcgccga ctccctcctg tgcgtcatgg cgcagcgcct
    10561 ggtgcggcgg ctctgccggc attgccgtgc cccctatcat cccgatccgg aagagtttgc
    10621 cgatctggtg cggaattatg gggagaaaga atttcaacgg ttcccgtttg cctacgacga
    10681 gacgacgacc ctgtaccggg ccggaggatg tccggagtgt cacgacacgg gctaccgggg
    10741 gatgatggga ctctacgaac tgctgattgc cacggacccg atcaaacaga tgatcattgg
    10801 ccggaaatcc atcgccgccg tgagaaaaac ggccatgggg gaggggatgc ggaccctgat
    10861 gcagagcggc atcctgaagg tcatcagcgg cgatacggat ttcgtggaag tcctcagcgt
    10921 ctgcatgcgc tgaacgcccg cccggaaaat attagatttc ttccttgact ctcttccggg
    10981 gctgtgttag cgtgacgctt catcgaatat gtatcataaa aacaatgaga tataaagttt
    11041 tatccctccg gaggagcctt ggatttttat cagcaggccc gaaatttact tgacgaaggg
    11101 aactataccc ggtccttgca tctggcgcag gaacggttgc gggccgctcc ggaggacgtc
    11161 ccggccaccc tggtcctgtg ccgctcctgg ttggggctgg gccgactgga ggaggcggcg
    11221 gcggccttcg cggcgatcga accgatgaac gaggaaatcg cccggctctt gaagctgctg
    11281 ggagacgcct tccaggggcg gggcgaccac gggcgggcgg tgaaatgcta tcagcggagc
    11341 atggccctcc ttcccggcgt cgaggaactg cgggaggtgc agaactctct ggcggagctc
    11401 atggaggacg atgaggagcc cacggccgga cccgccggcg gggaagggtt ccagaccctc
    11461 accatggcgg aactccacat tcgtcagggt cactacgaag gggcccggga gatcctaacg
    11521 gcgatcctgc tccgggagcc ggagcaccgg gaggcccggc ggcgcctcac cgaggtccag
    11581 gaacagataa acaaaaaaaa ctgtgatctt cctattgaca aaaaaaccac ggttgtgcat
    11641 gaactgtcgc ggtggttgat caaactcggc gggaagggac aatgacggac gacgcggcgg
    11701 atatagacag aaagaggatc gaacgggtct atgaaaggat cgacccgaag gaattcgatg
    11761 cgatcctctt ttttgacatg aataatatcc gttatttgac cggtttcacc ggcagcgacg
    11821 gggtcttgct cctccagccc gaccgggtga tgctgctggt ggacgggcgc tatgtcaccc
    11881 aggccgcgga gcaatccccc cattgcgacg ttgtccagta ccgcgacaag gtcagcggca
    11941 tcgcgacggc gctggcggaa cggggggcgg ggcgcgtggg cttcgaggct ttcgccctca
    12001 ccgtgggact ctatgaaaaa atccgggaac ggatccggac gacgcggctg gcgcctctcg
    12061 acggggaact ttcctgtctg cggagccgca aggacgccgg agaggcctcc ctgctccgcc
    12121 gggccgtcga catcgccacg gaggccctaa cggacgtcct gccgctcctg cgtccgggac
    12181 ggacggaaag agaaatcgcc ctggagctgg attaccgaat gcgggcgctg ggttccgagg
    12241 aaaattcctt tcccaccatc gtcgcctccg ggccgaacgc tgccctgccc cacgcccggc
    12301 ccggggacag gaaactggcc cccgacgacc ccgtcatcat agattttggc gccgtttacc
    12361 gtggttatca ctccgacgag acatgcacct tggtggtggg ccgacccgac gccgaattcc
    12421 gccaggtgta cgatatcgtg aaggaggccc atgaccgggc gatcgccgcc gtcaggacgg
    12481 gggtctcggg ccgggagatc gacggcatcg cccggcggca tatcgaggag cagggatacg
    12541 ggccgcaatt ttcccacggg acgggtcacg gcgtcggttt ggatgtccat gaggacccgc
    12601 ggctgtcacc gctttccgat caggtcctgg ccgcggacat ggtggtgacc gtggagccgg
    12661 ggatttacct gccgggccgg tggggagtcc ggatcgaaga catggtgctg gtgggggagg
    12721 acggctgcga agtgttgacc aggatgccaa aagagctgaa aaacattgat ttataataac
    12781 ctatagcgaa ggatgcactg aatatgaagg tattcccaga cgatttgctt tacagtcgcg
    12841 agcatgtatg ggtcagggtc gaaggagacc tggccaccat cggcattacc gattacgccc
    12901 aggagaagct tggggaggtg ctggcggtgg atctccccga ggtggatacg gaggtggaac
    12961 gggatgaacc tttcggcgcg gttgaatcct ccaagggcgc cgtcgaactg atttcccccc
    13021 tgtccgggaa tgtgttgaac gtcaacgagg atctcatcga cgatatcggc atcgtgaaca
    13081 gcgaccctca tgataccggc tggatgatcg tcgtggagat gcacgacctc gacgaactgg
    13141 acgatcttct cgatgcccgg gggtatcacg acttcattct ccaggaaacg gaaggggact
    13201 gatcccggtg atgaattggc ggctcctccc gtttggccgg tatcccgccg gggaaaacat
    13261 ggccatcgac gaggccgtct tccgggaagt ggggcgcaac ggggg
//
LOCUS       DAOSMF010000043        13067 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_5551_length_13067_cov_16.101906, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000043 DAOSMF010000000
VERSION     DAOSMF010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 13067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13067
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5551_length_13067_cov_16.101906"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="PLT63_05965"
     CDS             complement(<1..283)
                     /locus_tag="PLT63_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98303.1"
                     /translation="MNPEDMARLQYAKDLLENPGLAAKLTNVIGIPFEKAFGYLPEKW
                     AATIQTITHKSLQHALEAAVKTLAREDRKSSSDKLHKFLVAASGGIGGAF"
     gene            complement(320..658)
                     /locus_tag="PLT63_05970"
     CDS             complement(320..658)
                     /locus_tag="PLT63_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98304.1"
                     /translation="MKLKTLLILKIGFTLIFLASSAVFAEECFNSTKKLNADAQTIRL
                     KAMDMGRKVGKTASLAAASIVKGKTELYPKDNVEICIREEGRALQIKAQSKSKDAGMA
                     EWHSITAKKQ"
     gene            complement(699..1040)
                     /locus_tag="PLT63_05975"
     CDS             complement(699..1040)
                     /locus_tag="PLT63_05975"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOD98305.1"
                     /translation="MAKSTDFVEKELKDFVALIRKKYDIYAVVLYGSYAEGRANDDSD
                     IDVAIFSDDFGKDPYEEMKALFRLRRQIDTDIEPLPFSRDAYFSDDKTEFVSDIIRKG
                     KVIYKENRLLI"
     gene            complement(1581..2081)
                     /locus_tag="PLT63_05980"
     CDS             complement(1581..2081)
                     /locus_tag="PLT63_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98306.1"
                     /translation="MECKPYQRRWRDWAVDRNLEDIWLARLNALETLDLVSVCEGHTD
                     AKPTSVKRRPSVILIVKESYLKPLTGRWYEIKAALAGELERIWPGNDTSIELAIQHTL
                     AREDGQRNDVEEIILRCFSSRQRDAAPFSDWVGRWFQNTVCRIEEYDRRFKKLMEQGV
                     QEQCQP"
     gene            complement(2124..3626)
                     /locus_tag="PLT63_05985"
     CDS             complement(2124..3626)
                     /locus_tag="PLT63_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD98307.1"
                     /translation="MDSLLLHIDNASNDLWVFFSDNGWSTIGGPFLYRFDSQSLKYKG
                     DIRLFNDKGKPATSRHEPLFATHDQSGNFILATDLAYLIGVDSRGKCTYRKRLMRHDC
                     RWTSRNMERKKLYFIRDIKKHGDYRDRLDYREKRIHSLNLMSMAYSVSRDLLFVGQGC
                     HCCSNDILVCNGQGEYLYHFRSPVGPEDMAARDGLLYLADNFHALIHVFTYEGKWLRS
                     LDVLLKNRIQGVIDPELDMFQQQWNTDGLFHVEDEDQVASLCVIGEDKLAVGLEKGLI
                     RILDKEGNHLFDIQPPKPEFYPKSMVADASENLFVYYTGDRYFNKPRGLYRYGANGKV
                     KGPLFGGELGIFKPAQKALEKKIAEDRADACDYFNLADILIRRKKPLRETISLLEKSL
                     ELKPDLWIALAYLGLTLKETGETERAVDCMERAMEHMGCSNMATALIEFYYRMNNREK
                     VWQYFKRMEAAEDDVDIEFYSSELTNNQLEEFLGTLVKRNKCDCLVSSKP"
     gene            complement(3747..3875)
                     /locus_tag="PLT63_05990"
     CDS             complement(3747..3875)
                     /locus_tag="PLT63_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98308.1"
                     /translation="MSIITRETKILLLVLVLAMMFVWLIFGFEILQYLGGPVLFFR"
     gene            complement(4387..4566)
                     /locus_tag="PLT63_05995"
     CDS             complement(4387..4566)
                     /locus_tag="PLT63_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98309.1"
                     /translation="MLHPNIRPISTPNLVRVVYGQILQQVRVTATIRKYPWQLLQQLL
                     TPLPYLVRMYAKFTG"
     gene            4781..5620
                     /locus_tag="PLT63_06000"
     CDS             4781..5620
                     /locus_tag="PLT63_06000"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HOD98310.1"
                     /translation="MRATAIVLRVMLVILLVIPFWAASCGAGEDLSLARAWEIIRTKR
                     FVDLTHTFAPGIPHWKGFPDEKRETLYGYEPGAGKLGSGFYAQAFTLVGQWGTHADPP
                     AHFVKGLRTLDQIDVKEMILPLVVIDVHEKAARNPDYTITLDDVREWERRNGPIPGGA
                     FVAMRTDWSKRWPDPKAMANADEKGIAHYPGWSLPVLKYLYEERKIAASGHETTDTDP
                     GIATSKDDYILETYVLKQNRYQIELLANLDQVPQAGALVVVTFPKPKDGSGFPARVFA
                     ILP"
     gene            complement(5727..6224)
                     /locus_tag="PLT63_06005"
     CDS             complement(5727..6224)
                     /locus_tag="PLT63_06005"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOD98311.1"
                     /translation="MPEPAFCLYCGEKLTTKQIEGRDYRACSREGCGFVYWDNPLPVV
                     AAIVEHEGKVLLARNKLWPVKMYGLITGFLEKGESPEGAVLREVKEELGLDGKLVGLV
                     GLYAFRERNQLIIAYHVTAAGEIRMNEELAEVISVPPEQLRPWSFGTGHAVKDWLKMR
                     GIAAG"
     gene            6486..7820
                     /locus_tag="PLT63_06010"
     CDS             6486..7820
                     /locus_tag="PLT63_06010"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOD98312.1"
                     /translation="MLHFKTYRWVLLGIIAILYFFVCLQRLSPSVVARDLALALHADA
                     VLLGIIASAYFYLYSAMQPVVGYLSDTKGPRKVMALSFLIAAAGTILFGMAPNATAAI
                     VGRTLIGAGLAGVFIPALKVFSRWYRPDQFAGLTGIMITIGGIGGLSAALPLTYLVVL
                     LGWRGAFLAIGLLSAVLAVLAWFIVRDSPEDRGWPPLMAQGGGGAVPETKMGEIGLSK
                     RLGTVFGDLDFWLISIASFLAFGASLSFQGLWAVPYLMDVFHLDRVRAGWVLTAMPLG
                     FSIGGPAVGFLADRLHLNRKKVLLWAIGLSILGWLLLMAFQDLGYLVPVVPLFFFFGL
                     AAGGMLPLFFTITRDIFPLWLMGTATGLMNTSAFFGTAVYQPFTGLLLNDAASTQPGS
                     YSFDAYRMLLIVYLISYGGAFLAMCFLSKRGVRPPSGGAAETGRHPVQPGTE"
     gene            complement(7984..8388)
                     /locus_tag="PLT63_06015"
     CDS             complement(7984..8388)
                     /locus_tag="PLT63_06015"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HOD98313.1"
                     /translation="MKTLRTLHAALAVATLAICLFCMAPAAALTVSYTYDGAGRLIKM
                     AFGSGQRILYTYDAGGNILKRQSFDRGSDLYGDVNGDGDVNLTDMVTVLQLQADLDRT
                     REYYNLKADVNGDNRIGSAEAVYILQKTAELR"
     gene            complement(8407..>9184)
                     /locus_tag="PLT63_06020"
     CDS             complement(8407..>9184)
                     /locus_tag="PLT63_06020"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HOD98314.1"
                     /translation="GAMTDAYAYDPYGRILRRTGTSSQPFTFAGQWGMRQEDATLYQA
                     RARYYDAATGRFLSREPLWPQTDDPRLLNPYSYARANPVLEVDVTGREPEKPDNITPV
                     HMEQGLAPDSWTTTIHNSIETAIVKKSYSTTDKGRRFGVMAGNEHKKEMNQEQERNRK
                     IGKKANMIKFGKPLFVNVADDGDRIVVLIFVRKNNLLVVKDMFEIGKKELEAGEPQAD
                     FSLRERLFGSDVKRVEANNPVADRFVDNVAASVRRHVDDE"
     assembly_gap    9185..9284
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9285..12809)
                     /locus_tag="PLT63_06025"
     CDS             complement(<9285..12809)
                     /locus_tag="PLT63_06025"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98315.1"
                     /translation="MNKLFRNSWPKGTTALIILAVVLGCDTATAGGPYGTCAQFGVEE
                     HQCFCDGNQKRICSDGYVWDPQPDECTSLSCGDPPPPPPPPKINVPGKTKPKGISKKI
                     TPKVGDPISAAGGEFRETWTLLSLGGPIPLEFTLAYAPDLVDKVPSSDGRLPFPPTDS
                     LKAFTTSAVMRLVEFTDKGVSPGIDYVNILLFDDLLVFRYDTAAAAYLPVGPVRYELK
                     KTASGQYFLMDPEQELIYLFRSRALAYERTSPPQTVQRVGEIEYIMDRNANVISYTYN
                     ENYLPTKIQDEGSDRVLNLTYDGNGHLARVADGYGRSVIFKIETLFCGGLPWNGLSYF
                     SDAMARRTTFTYDMSTYPCYLLNKIERQRGNSHIDQAWTDNPAGVKGVSSQKDAYGHE
                     TALSWLRDDAGNLTTTVTYPGGARQVFSSQDERYPLGLKDPGDQTVSMGYTEDHRLSR
                     ITDTLGFTTAMTYHPQSGKLASVTNAEGKTTTYAYTAQTQSFAGGAATFTFYDLTRTD
                     YPDGTLETFAYDAKGNLTSRTDRRGNTWSFAHNAKGQVLTATNPLGGVETFTYNNDGT
                     LASAKDSDTGTTGYDYDDHKRPIKVTHPDGTFLQITYNANDEIVFVTDENNHTSTYVY
                     NSNGHLAEAQDHLYNVTTYAYDNMDRIRQITDKLGKITTLTFDNRGRVASITDPDSVT
                     TTYGYDTYGRQNSVARGDAVWQTTYDSEGLPSLSKTPLNRQTRYESDRLGNLTAVTDP
                     TGSRMVLSRNAMGQVTGITDGLSRQTGYAYDAAGSLAGVTLPEVGGAAYERNALGLIG
                     RITDLNGKTWIFAYTPMGRLQGLTDPLNRTWQYAYDNRGRLSGVTFPDGATKTITYDG
                     VGNIVQAAHSAGPTLSFGYDDFQRLSSTASLTLSRDAMGRVLTTSDGEASFTASYTNG
                     GRLKSLEVSANDDRFSVTYAYNAQTGLLEGVSDSLTQTAVTFAYDADGHLTAIDRPNG
                     ANTRYDYDAAGRLVRIREGSFLDAAYTLDPVGQVVRADMTLPLDPVPAVGDSTEAFSY
                     NDASQTASAGYEYDPQGRLTAAPGRTLTWDGASRLTAVGGVQMGYNGMGDLITRTDGA
                     ATTRFSYSHSLGRHPLVAERNTSGPALARYYVWTPDGRLLYLIDRTNGTKAYHYHFDR
                     IGSTIALTDGSGAMTDAYAYDPYGRILRR"
     gene            complement(12836..>13067)
                     /locus_tag="PLT63_06030"
     CDS             complement(12836..>13067)
                     /locus_tag="PLT63_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98316.1"
                     /translation="GPPGDHPQFHQVVRSEGRTEICPDEVSKLRGVVFIIDFIFSVNV
                     VWVKAGMEGEHRHRRLTSRNNRANEVSGINQS"
BASE COUNT         2507 a         3547 c         3728 g         3185 t
ORIGIN      
        1 cgaaagcgcc gccgatgccg ccggatgcgg caaccaggaa tttgtgcagc ttgtcactgg
       61 atgattttct gtcttcgcgg gcaagcgttt tgacggctgc ctccagcgcg tgttgtagag
      121 atttatgcgt tatcgtctgg atcgtggccg cccatttctc cggcaggtag ccaaaggctt
      181 tttcaaatgg tatgccgatc acattggtca gttttgcagc cagtcccgga ttttccagca
      241 ggtctttcgc atattgaagt cgtgccatgt cttccggatt catttccata atcttacggg
      301 cgttaattgc cgctgctttc tattgttttt ttgcagtgat actgtgccac tccgccatcc
      361 cggcatcttt tgatttggat tgcgccttga tctgaagggc gcggccttct tccctgatgc
      421 agatttcaac gttgtctttg ggataaagtt ctgttttgcc tttgacgatg gatgcggcgg
      481 caagggaagc tgttttgccg accttcctgc ccatatccat cgctttgaga cggatcgtct
      541 gggcgtcggc attgagtttt tttgtggaat taaagcattc ttcggcgaaa actgctgagg
      601 aggcaagaaa gatgagggtg aagccgattt ttaaaatcag gagtgttttt agcttcatga
      661 tgattccgtt aacaccaacg gcctttatgt tcgcttgttt atatcaacag gcggttttcc
      721 ttgtaaatga ctttgccttt cctgatgatg tcgctgacaa attccgtttt gtcgtcgctg
      781 aaataggcgt cccggctgaa gggcaaaggt tcgatgtcgg tatcgatctg ccggcgaagg
      841 cggaaaagcg ccttcatttc ttcatagggg tccttgccga agtcgtctga aaaaatggca
      901 acgtcgatgt cgctgtcatc gtttgcccgg ccttccgcat aagatccgta aaggacaaca
      961 gcgtaaatgt cgtatttttt cctgatcagg gcaacgaagt ccttcaattc tttttctaca
     1021 aaatccgtgc ttttagccat tggagcagct cctctgtttt atcaggaccc agtattggat
     1081 tgtcttttca ctcatgactc acgtcctgta catcgtgcgt gggcactgct cttttatcct
     1141 gcgattttaa cctgatggtt cgtgcgttat ttaatacaat aaaagaatag attccgcaac
     1201 ataatgattc ccaacaatga ttttcaatgc ggttgccaaa ttcccatcat ctgaaaaatg
     1261 gatgatgatc tccgctttcc ttgccctgcc gacaaaacgg ggaacctctt gaataagtaa
     1321 cggttttccg aggttttctg cggatcaggt ttctgctcca ttgcggccca ggtcatgccc
     1381 ccggagcgat acaggccaag aacggcgttt ctggtgaagg attcccgtgt ccaggcagcc
     1441 agaggcgcaa aggaatgata caaagaagcc ttttccctga cgaagcatgt atggttgatg
     1501 ggtaaacagt tctggtttgt cggcggccca ctgcaaaaaa agctttttca aatagctgcc
     1561 tctctcgatt atttcggtgc tcatggttga cattgctcct ggactccctg ttccatcagc
     1621 ttcttgaagc gccggtcata ttcctctatc ctgcaaacgg tattttgaaa ccaccggccg
     1681 acccagtctg aaaacggcgc tgcatccctt tgccggctgg aaaagcagcg aaggatgatt
     1741 tcttccacat cgttcctttg accatcttcc cgggccagcg tatgctggat tgccaattcg
     1801 atgctggtgt catttcctgg ccagatcctt tctaattcac cggcgagagc cgccttgatc
     1861 tcgtaccatc tgccggtcag cggcttcagg taagattcct tcacgatcag gatcacggaa
     1921 ggtcttcgct tgacggacgt tggttttgca tccgtatggc cttcgcagac gctgacgaga
     1981 tcaagcgttt ccagggcgtt caggcgcgca agccagatgt cttccagatt ccggtcaacg
     2041 gcccaatccc gccagcgtct ttgatagggt ttacattcca tcgatggctg ctttcctgtt
     2101 ccaacatatt cacttcctcc tttttatggt ttactggata ccagacaatc gcacttgttc
     2161 cttttcacta atgttcccag gaactcttcg agctgattat tagtcagttc ggagctgtaa
     2221 aattcgatgt ccacatcgtc ttccgctgct tccatccttt tgaagtattg ccagaccttt
     2281 tccctgttgt tcatgcggta ataaaattcg atgagcgcgg tggccatgtt gctgcacccc
     2341 atgtgttcca tggccctttc catgcaatca accgcccgct cggtttctcc cgtttccttc
     2401 agcgtcagcc ccagataagc cagggcaatc cacagatcgg gtttcagctc cagggacttt
     2461 tccagaagcg aaatggtttc ccgcaatggc ttcttgcgcc ggatcaggat gtctgccaga
     2521 ttgaaatagt cgcaggcgtc ggcacggtct tcggcgatct tcttttccag ggctttttgc
     2581 gcgggcttga agatgccgag ttctccgcca aaaagaggcc ctttgacctt cccgtttgcg
     2641 ccataacgat agaggcccct gggcttgttg aagtaacggt cgcctgtata atagacaaaa
     2701 agattttccg acgcgtcggc gaccatgctt tttggataaa attcgggctt aggcggctgg
     2761 atgtcaaaaa ggtgatttcc ttccttgtcc agaattcgga tcagcccctt ctccagcccg
     2821 actgccagct tgtcttcgcc gatgacgcaa agagaagcaa cctggtcttc atcttccacg
     2881 tggaaaaggc cgtcggtgtt ccattgctgc tggaacatgt cgagttcagg atcaatcact
     2941 ccctgaattc tgtttttcag caaaacgtcc agagagcgca gccacttgcc ttcgtaagtg
     3001 aaaacatgga tcagggcgtg aaaattgtcg gccagataca aaagaccgtc gcgggcggcc
     3061 atgtcttccg gccccacggg cgacctgaaa tgataaagat actcgccctg gccattgcaa
     3121 acaagaatgt cgttgctgca gcagtggcag ccctggccga caaagagcag atcgcgggac
     3181 accgaatagg ccatggacat cagattaaga ctgtggattc ttttttcccg gtaatccagg
     3241 cggtcccgat aatcaccatg tttttttatg tccctgataa aatataactt cttgcgctcc
     3301 atgttgcggg atgtccatct gcaatcatga cgcatcaggc gttttcgata agtgcatttt
     3361 cccctcgaat ccacgccgat caggtaggcc agatcggtgg ccagaatgaa gtttccactt
     3421 tgatcgtggg ttgcaaacag tggctcatga cgactggtgg caggtttccc cttgtcgttg
     3481 aaaagacgaa tatccccttt atatttaagg ctttggctgt cgaaacggta aaggaacggt
     3541 cctccgatgg tggaccatcc attgtcggaa aaaaagaccc acaaatcgtt gcttgcgtta
     3601 tctatatgca ggagcaggct gtccattttt ttactcctta ataaacgaca acagtccagt
     3661 tcagcagata atagcccgcg atcaacgcgg cgatcactgc tatggcaatg acaatccttt
     3721 taaaagtcat gattctgtcc tttcctttat ctgaagaata aaaccggccc gcccaggtac
     3781 tgcaaaatct caaaaccgaa gatcagccag acaaacatca tggcgagcac cagtaccagt
     3841 aataaaatct ttgtttctct cgtaatgatt gacatggccg tttcaacctc cctttgactg
     3901 acatattata tagagttggg gcgtctgtca agatatttta tcaataattt caatatctta
     3961 atgtgaagtt catataaaaa ttacaatttc tatatgaacg gcgctgaata ttaataatgt
     4021 tacttatgct tttgatattg ttagctttgt gaaataacgg tcgaaaatga aaaataaaaa
     4081 atgatgaaat ttttttgcct cgttaagatt tcatcggccg gatctcgccg ccgcaaaata
     4141 ttcatataaa aattaattat ttgagcctgt tcaataattc aaagttttag gacggggctt
     4201 ttctttccgg aatagaggta gtcgaaaatg ctccccgtaa actggacagg tgtataaggt
     4261 gcatgttagc ctgcatagag aaggaggagg caggagatgg tcagggggat gcggaagagc
     4321 cacgatggtg ccttcaaggc gagggttgca ttggaagcct tgaaagggga gaagacgatg
     4381 gcgcagctat ccagtgaatt tggcgtacat gcgaaccaga tacggcagtg gcgtcagaag
     4441 ctgttggagg agttgccagg ggtatttacg gatcgtcgct gtcacccgta cctgctgaag
     4501 aatctgacca tagaccaccc ggaccaggtt tggtgtgctg attggacgta tattcggatg
     4561 cagcaatgtt ttatccccat gcaaacctct tgggccagtt ttgcattgac acaaaggggc
     4621 tccccctgtc ctcattcgtc aaaaaagcaa gcttttacca atcattacga atcgtggctg
     4681 ctgtgttttt cccctctccc ccgatccgtt tttcggtttt gcggtgacct ttgaacctga
     4741 aatatagaac cttgaacgac attttgacaa aggagacgag atgagagcaa cggccatcgt
     4801 cttgagggtc atgctggtga tcctgctggt gattcccttc tgggcggcct cttgcggggc
     4861 cggtgaggat ctttccctgg cgcgggcctg ggagattatc cgaacgaagc ggtttgttga
     4921 cctgacccat acctttgccc cggggattcc gcactggaag gggttccccg atgagaagcg
     4981 ggaaaccctc tacgggtacg aaccgggtgc aggcaagctg ggaagcggct tctatgccca
     5041 ggctttcacc ttggtgggcc agtggggaac ccacgcggac cctccggccc atttcgtgaa
     5101 aggcctgcgg accctcgacc agatcgatgt gaaggagatg atcctgcccc tcgtggtgat
     5161 cgatgtccac gagaaggcgg ccaggaatcc cgactacacg atcaccctgg acgatgtgcg
     5221 ggagtgggaa aggagaaacg gacccatccc cgggggagcc tttgtggcca tgcgcaccga
     5281 ctggtcgaag cggtggcccg atccgaaggc catggccaac gccgatgaga aggggattgc
     5341 ccattatccc ggctggagcc tgcccgtgct gaaatacctc tacgaggaac ggaagattgc
     5401 cgcctccggc catgaaacca cggacaccga cccggggatc gccacctcga aagacgacta
     5461 tatcctggag acctatgtgc tgaagcagaa ccgctaccag atcgaactcc tggccaacct
     5521 ggaccaggtc ccccaggccg gcgccctcgt ggtggtcact ttcccaaaac cgaaagacgg
     5581 ttcaggcttc ccggcccgcg tgtttgccat ccttccatag agggggccaa ggacgaatcc
     5641 gggacgtggg tgctttaccg gtaaaccgag ggcgttggtc ggcgtcgatg gtcatcgaac
     5701 cctggccgga gatgatgaag tggatgtcag ccagccgcaa tgcctcgcat tttcaaccag
     5761 tctttcacgg cgtgcccggt tccgaaagac cacgggcgca actgctcggg cggtacggat
     5821 atcacctcgg ccagctcttc gttcatccgg atctcgcccg ccgcggtgac gtgataggcg
     5881 atgatgagct ggttcctttc ccggaaggca tacaggccga ccaggccgac cagtttgccg
     5941 tccaggccga gctcctcctt cacctccctc aggacggcgc cttccggaga ctctcccttt
     6001 tcgagaaaac ccgtgatcaa tccgtacatc ttcacgggcc atagcttgtt gcgcgccagg
     6061 aggacctttc cctcatgctc gacgatggca gcaacaacag gaagggggtt gtcccagtag
     6121 acaaaaccgc acccctcccg ggaacaggcg cgatagtctc gtccctcgat ctgtttcgtg
     6181 gtcaattttt ccccacagta aaggcaaaat gcaggttccg gcatgattca atacccccgg
     6241 ttatggatgg atcgcaacgt cctttttttt acggacggag catgcgtcag ggcgttgccg
     6301 tatgtcaagc aaaagggtga ggattcagcg cagggggagg agctctccac ctcccggatc
     6361 cttttcccga tgcctttttc atcgtgcatg accgcatcta tcgctgcatc tattgcttga
     6421 caggcggctg ttcgttcact aaggtttcat aaacaaaaga caattcgaga agaaaggtgc
     6481 aacccatgct gcatttcaag acctatcggt gggtccttct gggtatcatc gcgattttgt
     6541 acttctttgt ctgccttcag cgcctttcgc cttccgtcgt ggcccgtgat ctggccctcg
     6601 cactccatgc cgatgcggtc cttctgggca tcatcgcatc ggcctatttt tatctgtact
     6661 cggccatgca gccggtcgta ggctatctct ccgacaccaa gggaccgaga aaggtcatgg
     6721 ccctgagctt tttgatcgcc gcggcgggca ccatcctctt cggcatggcg cccaatgcaa
     6781 ccgcggccat tgttgggcga accctcatcg gcgctggcct ggccggtgtc tttatccctg
     6841 ccctcaaggt attttcccgg tggtaccgcc ctgaccagtt tgccggcctc acggggatca
     6901 tgattaccat cggcggcatc ggggggctct ccgcagccct gccgctgacc tatctggtag
     6961 tcctcctcgg ttggaggggg gcattcctcg ccattggcct gctttccgct gtcctggcgg
     7021 tcctggcctg gttcatcgtc cgggattccc cggaagacag gggatggcct cccctgatgg
     7081 cccaaggagg cggaggcgcc gttcccgaaa ccaaaatggg ggaaatcggt ctttcgaaac
     7141 gactgggcac cgttttcggg gatctggatt tctggctgat ttccattgcc tcgttccttg
     7201 cctttggcgc ctccctttcc ttccaggggc tatgggccgt tccgtatctg atggatgtct
     7261 tccatctgga ccgggtccgg gccggatggg tgctgacggc catgcccctg ggattttcca
     7321 tcggtggacc ggcggtggga tttctggcag acaggctgca tctgaaccgg aagaaggtgc
     7381 tcctgtgggc cataggattg agcatcctcg ggtggttgtt gctgatggcc tttcaggacc
     7441 tgggatatct ggttcccgtg gtccccctgt tcttcttttt cggcctggct gcaggaggca
     7501 tgcttcccct gtttttcacc atcacccggg atatctttcc cctctggctc atgggaacgg
     7561 cgacgggcct gatgaacacc tccgccttct tcggtaccgc cgtctatcag cccttcacgg
     7621 ggctcctcct caacgacgcc gcatcgaccc aaccgggcag ctacagcttc gatgcctacc
     7681 ggatgctgct gatcgtgtac ctgatctctt acggcggggc ctttctcgcc atgtgttttc
     7741 tgtccaaacg gggcgtccgg cctccttccg gcggcgccgc agagacgggg cgccatcccg
     7801 ttcagcccgg tacggaataa ccgtcgcccg cgaaacccgc cccgtcgatg gggaaatccg
     7861 gcgccggatg ggtctttcct caggtgaagg gcgttaaaag gctattcgta aagagggggt
     7921 tatctgtggt acatagatgg cccccttttc ccatgcccgg aagagggggc ctgccttgtc
     7981 ggcctatcgc agctccgccg tcttctggag gatatacacc gcctcggcgg aaccgatccg
     8041 gttgtcgccg ttcacgtcgg ccttcaggtt gtaatactcc ctcgtccggt ccagatcggc
     8101 ctggagttgc aacacagtga ccatgtccgt caggttgaca tctccgtcgc cgttcacgtc
     8161 gccgtagagg tcgcttcccc tgtcgaagga ctggcgcttc aggatatttc cccccgcatc
     8221 gtaggtgtac aggatccgtt gcccgcttcc gaaggccatc ttgatcaggc gtcccgcccc
     8281 gtcgtaggta tagctgacgg tcaacgctgc ggcaggcgcc atacaaaaca gacagatcgc
     8341 cagggtggca acggccaggg cggcatggag agttcgcaag gttttcatga aggacctcct
     8401 ccttccttat tcgtcatcga catggcgtct gaccgaagcc gccacattgt ccacgaagcg
     8461 gtcggccacg gggttgttcg cctccacccg tttcacatcg cttccaaaca gccgctcccg
     8521 caaggagaaa tccgcctggg gctcccctgc ctcgagttcc ttcttgccga tctcgaacat
     8581 gtccttcacc acgagaaggt tgttcttacg gacgaaaatg agcacgacga tccggtcccc
     8641 gtcgtcggcg acgttaacga acaggggttt gccgaatttg atcatattgg ccttcttgcc
     8701 gatcttcctg ttccgctcct gttcctggtt catctccttt ttatgttcgt tgccggccat
     8761 gacgccgaac cgccgacctt tgtcggtggt cgagtaggac ttcttgacga tggccgtctc
     8821 gatgctgttg tggatggtgg ttgtccagga gtccggggcc aggccctgtt ccatgtggac
     8881 aggggtgatg ttgtccggtt tctccggttc ccggcctgtt acgtccacct ctaggacagg
     8941 gttggctcgt gcgtaggaat aggggttgag aagcctcgga tcgtctgtct ggggccacag
     9001 gggttccctg gagaggaaac ggcccgtggc ggcatcgtag taccgggctc gggcctggta
     9061 gagggtggcg tcctcctggc gcatgcccca ttgcccggca aaggtgaagg gttgggagct
     9121 ggtcccggtt cgtctcagga ttctgccgta ggggtcgtag gcgtaggcgt ccgtcatggc
     9181 gccgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntcgtct caggattctg
     9301 ccgtaggggt cgtaggcgta ggcgtccgtc atggcgccgc tcccgtccgt aagggcgatc
     9361 gtcgaaccga tccggtcgaa atgataatga taggccttgg tgccgtttgt ccggtcaatc
     9421 aggtagagca ggcgtccgtc gggggtccac acatagtagc gggccagggc cgggccggag
     9481 gtatttctct ccgccacaag gggatggcgc ccgagggaat ggctgtagga gaaccgggtt
     9541 gtggcggcgc cgtcggtgcg ggtgatgagg tcgcccatgc cgttgtagcc catctggacg
     9601 ccgccgacgg cggtcaggcg ggatgcgccg tcccaggtca gggtccggcc cggggccgcc
     9661 gtgagcctcc cctgtgggtc gtactcataa cccgcggagg cggtttggga tgcgtcgttg
     9721 taagaaaagg cctcggtgct gtcgccgacg gccggaacgg ggtccagcgg cagggtcatg
     9781 tccgcccgga ccacctgccc cactgggtcc agggtgtagg ccgcatccag gaaggatcct
     9841 tcccggatgc ggacgaggcg gcccgccgcg tcgtaatcgt atcgggtatt tgccccgttg
     9901 gggcggtcta tggccgtcag gtggccgtcg gcgtcgtagg cgaaggtcac tgcggtttgg
     9961 gtgaggctgt ccgagacgcc ctccaggaga ccggtttggg cattgtaggc gtacgttacc
    10021 gaaaagcggt catcgttggc agagacctcc agcgacttta atcttccccc attggtgtag
    10081 gaggccgtga aggaggcctc cccgtcggag gtcgtcaaga cccgtcccat cgcgtccctg
    10141 gaaagggtca aactcgccgt ggatgaaagg cgctggaagt cgtcgtaacc gaaagagagc
    10201 gttggccccg cggaatgggc cgcctggacg atattgccga cgccgtcata ggtgatggtc
    10261 ttggttgccc cgtccgggaa ggttaccccg ctcaaccgcc cccgattgtc gtaggcgtat
    10321 tgccaggtcc tgttcagggg atccgtcagg ccttgcaggc gtcccatggg ggtgtaggca
    10381 aaaatccagg tcttgccgtt caggtccgtg atcctgccga tcaggccaag ggcatttctt
    10441 tcgtaggcgg ccccgccgac ttccggaagg gtgacgcctg ccaggctacc cgccgcatcg
    10501 taggcgtagc cggtctggcg gctgagaccg tccgtgatcc ccgtcacctg gcccatggca
    10561 ttgcgggaca ggaccatgcg gcttccggtg gggtccgtga cggccgtcag attgcccagc
    10621 cggtcgcttt catagcgagt ctggcggttc aggggggtct tggacaacga gggcagacct
    10681 tcactgtcat aggtggtctg ccagacggca tcgcctcggg ctacgctgtt ttgccgtcca
    10741 taggtgtcat agccgtaggt tgtcgtcacc gagtccgggt ccgtgatgga tgccacccgc
    10801 ccccggttgt cgaaggtcag ggtggtgatc ttgcccagtt tgtccgtgat ctgcctgatt
    10861 cgatccatgt tgtcgtaggc gtaggtggtc acgttgtaca agtggtcctg ggcctccgcg
    10921 agatggccgt tggagttgta aacgtaggtg gaggtgtggt tgttttcgtc cgtgacaaag
    10981 acgatctcgt cgttggcgtt gtaggtgatc tggagaaacg tgccgtcggg atgggtcact
    11041 ttgatcggtc gtttgtggtc atcgtaatca tagccggtgg ttcccgtgtc ggagtccttc
    11101 gccgaggcca gggtgccgtc gttgttgtag gtgaaggtct ccacgccccc cagggggttc
    11161 gtcgccgtca ggacctgccc cttggcgttg tgggcaaatg accaggtgtt tcccctccgg
    11221 tcggtgcggc tggtgaggtt gcctttggcg tcataggcga aggtttccaa cgtcccgtcg
    11281 gggtagtcgg ttcgggtgag gtcgtagaag gtaaaggtcg cggcgcctcc ggcaaaggac
    11341 tgggtttggg ccgtataggc gtaggtggtg gttttcccct cggcgttggt gacggaggcg
    11401 agcttgcccg attgcgggtg gtaggtcatg gcggtggtga aacccagggt gtcggtgatc
    11461 cgggatagcc ggtgatcttc cgtatagccc atggacacgg tctggtctcc ggggtctttc
    11521 agacccaggg ggtaccgttc gtcctgggag gaaaagacct gccgggcgcc cccgggatag
    11581 gtcacggtgg tggtcaggtt ccccgcgtcg tcccgaagcc aggacagggc cgtttcatgg
    11641 ccgtaggcgt ccttctggga ggagacgccc ttgacgcccg ccgggttgtc cgtccaggcc
    11701 tgatcgatgt gggagttgcc tctctgacgc tcgatcttgt tcagaagata acagggatag
    11761 gtggacatgt cataggtaaa cgttgtcctt cgtgccatgg cgtcgctgaa atacgacagc
    11821 ccgttccagg gcagaccccc gcagaacaag gtctcgatct tgaagatgac ggatctcccg
    11881 tatccgtcgg caacccgggc aaggtggccg ttgccgtcgt aggtgaggtt cagaacgcga
    11941 tcggagcctt cgtcctggat ctttgtgggg aggtagttct cgttgtaggt gtagctgatc
    12001 acgtttgcgt ttctgtccat gatgtactcg atttcgccga cccgttggac cgtctgcggg
    12061 ggggaagtcc gctcataggc cagggcccgg ctccgaaaca ggtaaatcag ctcttgctcc
    12121 gggtccatca ggaaatactg accggaggcg gtctttttca gctcgtatcg cacggggcct
    12181 accggcagat aggcggccgc ggccgtatcg tagcgaaaga caaggagatc gtcgaacagc
    12241 aggatattga catagtcgat gccgggggag acgcctttgt cggtgaattc caccaggcgc
    12301 atgacggcgc tggtcgtgaa tgccttgagg gagtctgtcg gggggaacgg tagccgcccg
    12361 tcgctggagg ggactttgtc gaccaggtcg ggcgcgtagg ccagggtgaa ctccagcggg
    12421 atcgggccac ccagggacag gagggtccag gtttcccgga actcgccgcc ggccgcggaa
    12481 atggggtctc ccaccttggg ggttatcttc ttggagatcc ccttgggctt ggtcttgccc
    12541 ggcacgttga tcttgggggg cggcggcgga ggcggcggat cgccgcagct cagactggtg
    12601 cactcatcgg gttgaggatc ccagacatag ccgtcggaac agatcctctt ctggtttccg
    12661 tcgcagaaac attgatgctc ttccaccccg aactgggcgc aagttccata ggggccgccg
    12721 gccgtggccg tgtcgcatcc taaaacgacg gccaagatga ttaatgccgt cgtgcctttt
    12781 ggccatgaat tccggaatag cttattcaac acaatactcc tcaaaagaca tatctttacg
    12841 attggtttat acccgatact tcatttgcgc ggttgtttcg ggatgtcagc cggcggtgcc
    12901 gatgttctcc ctccatacct gctttgaccc ataccacatt aactgaaaat atgaaatcga
    12961 taatgaatac aacccccctg agcttgctca cttcgtcagg acaaatttct gttcttcctt
    13021 cggaacgtac cacttgatga aattgtgggt gatcccctgg ggggccg
//
LOCUS       DAOSMF010000044        13009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_5581_length_13009_cov_21.312490, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000044 DAOSMF010000000
VERSION     DAOSMF010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 13009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13009
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5581_length_13009_cov_21.312490"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="PLT63_06035"
     CDS             <1..319
                     /locus_tag="PLT63_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98317.1"
                     /translation="PSLITGVWVGFDQERSLGHQEVGGRAAAPIWLYFMSQSLAGQPI
                     ETFPVPEGIVFVKVNPKTGEPSSGGGSIYESFLEGTTPLGAIPVEAGQVKPEEMLPRE
                     ENE"
     gene            966..2663
                     /gene="ilvB"
                     /locus_tag="PLT63_06040"
     CDS             966..2663
                     /gene="ilvB"
                     /locus_tag="PLT63_06040"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HOD98318.1"
                     /translation="MQLRGAEILIKVLKQEGIHTIFGYPGGVLLDIYDELHKGQIRHI
                     LVRHEQGAVHAADGFARASGQVGVALVTSGPGASNTVTGIANAYMDSIPIVVLTGQVP
                     THLIGTDAFQEVDIVGITRPCTKHNFLIKRVDELAKTIHEAFYIARSGRPGPVLIDLP
                     KDVMAAVTEYEPQTPRKVEEAKPRPASKKAFYVMDLIAESTKPMILSGGGVILGGAAE
                     ELRKMAWLTKMPVSSTLMGLGAFPGSDPLWLGMPGMHGTYYANMAISHCDLLIAIGMR
                     FDDRVTGKIDHFAPNAKIVHVDVDPSSINKNVMVDVPIVSDCKSALQSFNALLEQQKF
                     SVDDEQRREWFDQIAAWRKENPLSYCQSDTEIKPQFVIETLYKLTKGKAIITTEVGQN
                     QMWAAQFYNYDEPNTFLSSGGLGTMGYGFPAALGAKIAFPDKLVVDIAGDGSIQMNIQ
                     EMATAIQDNIPVKIVLLNNGYLGMVRQWQQLFYGKRYSHTDMTYQPDFLKLAEAYGWL
                     GLRATRPDEVEAVLKQGLEAPGPALMDFRICREECVYPMVRPGGPIHEMALGREMAN"
     gene            2696..3181
                     /gene="ilvN"
                     /locus_tag="PLT63_06045"
     CDS             2696..3181
                     /gene="ilvN"
                     /locus_tag="PLT63_06045"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF008864.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HOD98319.1"
                     /translation="MEKKEYTLALLVSNKPDVLARVAGILGGRGYNIETLCVDATLDP
                     AYSKIILTTIQDKTTITKIEKQLIKLIDVHKVTDLTKVESVTRELMLVRMKLSDTNKG
                     YLALIIDTFNGRVVTMNGDNCVIEITGDRSLIEKAAASLKPLGIDDMARTGIISLERQ
                     N"
     gene            3203..4249
                     /gene="ilvC"
                     /locus_tag="PLT63_06050"
     CDS             3203..4249
                     /gene="ilvC"
                     /locus_tag="PLT63_06050"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HOD98320.1"
                     /translation="MAKLNIGGVIEDVITAEEFTLDMAREALKGETVAIIGYGVQGRG
                     QALNMKDNGINVIVGEGDYNWQKAIDEGFVPGKTLFPPMEAAARGTIIQYLLSDAQQK
                     AIWPELKSHLTKGKALYFSHGFSIVYKEQTGVIPPPDIDVILVAPKGAGRTVRDNFLA
                     GSGINSSFAVFQDATGRARKRALATGVAIGSGYLFPTTFEMEVYSDLTGERGVLMGCL
                     AGVLEAQYNELRKRGHSPSEAFNETVEELTQSLMPLVAQNGMDWMYANCSTTAQRGAL
                     DWKNRFRDAVAPVFTELYDRVAAGKETEIVLKAGAAANYRDTLQKELDLMKNSEMWQA
                     GATLRALRPERRKK"
     gene            4440..6101
                     /gene="ilvD"
                     /locus_tag="PLT63_06055"
     CDS             4440..6101
                     /gene="ilvD"
                     /locus_tag="PLT63_06055"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOD98321.1"
                     /translation="MRSDLMKKGLERAPHRSLFKAMGYTNEEIGRPIIGVVNSANEII
                     PGHIHLRIIADDVKAGIRLAGGTPVEFPAIGVCDGIAMGHTGMKYSLATRELIADSVE
                     SMAMAHPFDALVMIPNCDKIIPGMLMAALRLNIPAIFVSGGPMLAGKLKGKPIDLISV
                     FEGVGKVKAGKISQKELNAIEDCACPGCGSCSGMYTANSMNCVTEALGLGLPGNGTVL
                     AVHAARRRLAKEAGMKIMQLLKKDIKPRDIATLAAFKNAIAVDMALGCSTNTVLHIPA
                     IAHEAGIDLDLGLFNEISKKTPNLCKLSPAGVHHIEDFDAAGGIQALMKRIHPLGVID
                     EQALTVTGKPVGENIKMAEVLDAEVIRSLDNPYIKEGGLAILKGNIAPEGCVVKQSAV
                     AENMLVNEGRARVFDAEDDAIQAILGGKIKAGDIVVIRYEGPKGGPGMREMLGPTSAI
                     AGMGLDTTVALLTDGRFSGGTRGAAIGHISPEAADGGPIALVQEGDIIAIDIPKKKIT
                     LKVTPEEFKKRKKEWKPKAPAITTGYLARYAKLVTSASTGAVFKD"
     gene            6355..6645
                     /locus_tag="PLT63_06060"
     CDS             6355..6645
                     /locus_tag="PLT63_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98322.1"
                     /translation="MKMFLVVYADYFDERISKTFMEAGFGKYTKVHGVTGRGEESEAK
                     LGTHYSPGKNNSIFMAVQDEEIPRLLEVIRGLRAEYPGSGFRAFTLPLEECI"
     gene            6813..7811
                     /locus_tag="PLT63_06065"
     CDS             6813..7811
                     /locus_tag="PLT63_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOD98323.1"
                     /translation="MTIKETNMAGHPADPVAPPGAVFMDVSVCVERDVSIRVMTWRPQ
                     RPVSEAPIIFVAGWVSAVSGWAPLLKVMAAVRPVYYIETREKRSARIEKKDLQPEDFS
                     IGRIAADLISVSRQLEIAVSNTTVIGSSLGATALLEALKGGELTAGAAFLIAPNSDFP
                     APWHIRWMLTLPAFLYHGLKHIVLWYVRNFMVDVKNEPEQMQRYTETLQAAHPQRIKL
                     SAQAAIDGRYEVWPHLETVRIPVALAYAPTDKLHSATNIRLIAARLPRAEIIVCPSNR
                     YMHRPELMADIEAFMRMTYYSQCSHQGPFYRDSMMGQRIIRGPAEDDGLTKDDKSS"
     gene            7824..8558
                     /locus_tag="PLT63_06070"
     CDS             7824..8558
                     /locus_tag="PLT63_06070"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmJ/YjtD family RNA methyltransferase"
                     /protein_id="HOD98324.1"
                     /translation="MISTHLDNFTVVLHKPKYPGNIGSTARCLKNMGMGKLIISEAGP
                     FSPAEMKIMSTHFAADIVDRIGYHDRLGDALAPFQYIVGTTSRLGSARGPVLSPREIS
                     PSLAELSRRNEVALLFGAEDKGLSNSDLRYCHSVVAIPVSEKLKSINLSHAVMILCYE
                     ISLAAAERKSVFTPKLASSREIEGMYEQVKEVLMEIGFLNPQNPDYWLMHIRRLLSRT
                     GLFSRDVKIIRGICRQVRWALRNKNS"
     gene            8648..8866
                     /locus_tag="PLT63_06075"
     CDS             8648..8866
                     /locus_tag="PLT63_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98325.1"
                     /translation="MIILKKLFLFMIALFLLPGLTGCWYAAAAGAGAYGGYKAKEAGY
                     SLQNPVTKKDSSKKSSPKETKKQTTTQQ"
     gene            8927..9496
                     /locus_tag="PLT63_06080"
     CDS             8927..9496
                     /locus_tag="PLT63_06080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HOD98326.1"
                     /translation="MSKEEKIAAIKQRARKNFTLGYNCAECIVEAVTGEIDTGLPPEV
                     KKVATGFGGGVGLYGDTCGAITGAIIAVGCVHGRGGLPEGEGKEVVKKAVNQLYGKPG
                     AYRLFNQIPIKFSDKYGNTLCRELTSKWHDSWLCRDHALHCREIITDAAEIAAELCLA
                     DYNEAASKPFGKNVENLKDPAADQSDKPT"
     gene            complement(9828..10025)
                     /locus_tag="PLT63_06085"
     CDS             complement(9828..10025)
                     /locus_tag="PLT63_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98327.1"
                     /translation="MAILKEAGIPIGRMLFIPKEGNLTRNDLEIESTGQYQFLERPDC
                     FVVKNAECCRSISVKVKTKES"
     gene            complement(10231..10812)
                     /locus_tag="PLT63_06090"
     CDS             complement(10231..10812)
                     /locus_tag="PLT63_06090"
                     /inference="COORDINATES: protein motif:HMM:NF021561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2284 domain-containing protein"
                     /protein_id="HOD98328.1"
                     /translation="MQNTSDATQKIASLIAFLKEQGATAALPLAVDRIVVDERVRLKC
                     RVPICDSFHKNLMCPPFVMSVTEFREALSRYQEAVVLQVAKEIAGLDGCRPHEDVYAP
                     AGRLHELVNRGEKEAFLTGFRFAAGLIGGCCRLCPVCVAAEGGMHCRFPFQARPSLEA
                     MGIDVVATLKNVGLSLAFPVQGRVTWTGLILLR"
     gene            complement(11022..11582)
                     /gene="cobO"
                     /locus_tag="PLT63_06095"
     CDS             complement(11022..11582)
                     /gene="cobO"
                     /locus_tag="PLT63_06095"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOD98329.1"
                     /translation="MAADNIIEATLAKFNRGIVMVITGNGKGKTTAAVGQALRALGHG
                     RKVFMIQFMKGKKYGEVLAAERCLPEFTIRPSGLDSFVMRDNPAPIDRELAQAGLEMA
                     RQVVAAGEHELVILDEINVALDFKLINLEDVIAVVANKPPAVDLILTGRYAPPEIVAL
                     ADTVSEIQEIKHHYAKGIKERAGMEF"
     gene            complement(11681..12655)
                     /gene="meaB"
                     /locus_tag="PLT63_06100"
     CDS             complement(11681..12655)
                     /gene="meaB"
                     /locus_tag="PLT63_06100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HOD98330.1"
                     /translation="MEKVKKIRGGDVRTASRLIRNLEDGMIEARSTIKHIFPLTGRAH
                     VIGLTGSPGAGKSTLVDGLVESFRKKEKTVGVLAVDPTSPFTGGAILGDRIRMQRHAE
                     DPGVFVRSLATRGALGGLAKAVGDAIHILDVMGKDKVIVETVGTGQQEVDIINHSHTV
                     LVVLIPGMGDEIQAIKAGIMEIADIFVINKADREGSSKLRREIMAMLDMAPPETFAAG
                     WRPPIIRVENAFEPDGFGKSLEELSATIEKHYQHLVQHNLIGSRVRRKAVVELNEAIR
                     NSVLEPVLNRLVQNGEIEGMIQKLINKETDPYTLAEDVARRYLKECSG"
     gene            complement(12660..>13009)
                     /locus_tag="PLT63_06105"
     CDS             complement(12660..>13009)
                     /locus_tag="PLT63_06105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HOD98331.1"
                     /translation="RGARVLARCFRDAGFEVIYTGCHQTPEQIASAAIQEDVDVVGLS
                     CLSGAHRYLFPAVAKLLKEQGADDITVVGGGIIPEQDFQMLYDAGLKAIWVPGATLDS
                     IVDWFRTNVQPRN"
BASE COUNT         2913 a         3876 c         3690 g         2530 t
ORIGIN      
        1 cccctcgctg atcaccggcg tctgggtggg cttcgaccag gaaagatccc tggggcacca
       61 ggaagtaggc ggccgggcgg cggccccgat ctggctctat ttcatgtccc agtccctggc
      121 tgggcagccc atcgaaacct tcccggtccc ggaagggatc gtgtttgtta aggtgaaccc
      181 gaaaaccggg gaacccagct ccggaggtgg ttccatctac gagagtttcc tggaggggac
      241 gacgcccctc ggggccatac ccgtcgaggc cggacaggtc aaaccggagg agatgctgcc
      301 ccgggaagaa aatgaataaa tgggttaatt acaccacttc caggaaggag cgctgggaaa
      361 cggacccccg ggtgttgcca catcaactgg gtgatcctca acagcgactg ggaggatgaa
      421 ttctgccgcg ttgccgaagc gcatccccgg gtccgggcct atgtaaaaaa ccaccatctc
      481 ggactggaac tcccttaccg ctacggttca gagacgcggc gctaaatctt tccaaaggtc
      541 tctgagcatg atcagaagag tcgccgccgc cctgggcgcc acgatccgta tcgagctgct
      601 gcgcggaaag caaaagaaga ccatgggcat cgctgaaaat gattcgcatt atggaaaaag
      661 gcaatgaatt aggatcaaaa aaattcttga caaaaactgc ttattttcgt acggtgcctg
      721 cactttttca gtttgaaagt ggataaatca tcaagatgaa aaacaaactc attgtactga
      781 gcagcgtcgt agtagttatc gtaccgaggg ggactgctgc tcggtgtttt taaacgacct
      841 ttaaaaacca gagccgcggg cccctgaaac ccgcggcttt tttgtttatg aaaagccgca
      901 tctccgaaca gatcgcggct tttttattgc cgacaaatca aacatccata agggaggtac
      961 ataccgtgca actaagagga gcagaaatac tgatcaaggt gttgaagcag gaaggtatcc
     1021 acacgatctt cggctatccg gggggcgtgc tgctggatat ctacgacgaa ctccacaagg
     1081 gccagatccg ccacatcctg gtgcggcacg agcagggggc cgtccatgcc gccgacggtt
     1141 tcgcccgggc ctccggccag gtaggggtgg ccctggtaac ctccggcccc ggggcgtcca
     1201 acaccgtcac ggggatcgcc aacgcctaca tggattccat ccccatcgtc gttttaaccg
     1261 gccaggtccc cacccacctc atcgggaccg acgccttcca ggaggtggac attgtcggca
     1321 ttacccgccc ctgcacgaag cacaacttcc tcatcaaacg ggtggacgaa ctggccaaaa
     1381 caatccatga ggcattttat atcgcccgct ccgggcggcc gggtcccgtc ctcatcgacc
     1441 tgcccaaaga cgtcatggca gcggtgacgg aatacgagcc tcaaaccccc agaaaagttg
     1501 aagaggccaa acccagacct gcttcgaaga aggcgtttta cgtcatggac ctgatcgccg
     1561 aatccaccaa gccgatgatc ctttccggcg gcggcgtcat cctcggcggc gccgcggagg
     1621 aactccggaa gatggcctgg cttaccaaga tgcccgtttc gtcaaccctc atgggactgg
     1681 gggcatttcc cgggtccgat cccctctggc tgggcatgcc ggggatgcac ggcacctatt
     1741 acgccaatat ggccatcagc cactgtgatc tcctgatcgc catcggcatg cgcttcgacg
     1801 accgggtgac gggcaagatc gatcacttcg cccctaacgc gaagatcgtc cacgtggacg
     1861 tggacccgtc ctccatcaac aagaacgtca tggtggacgt ccccatcgtc agcgactgca
     1921 agagcgccct ccagagtttc aacgccctcc tggagcagca gaagttcagc gtcgacgatg
     1981 agcagcgccg ggagtggttc gatcagatcg ccgcctggcg gaaggagaac cccctgagct
     2041 actgccaaag cgatacggag atcaaacccc agttcgtcat cgagaccctc tacaagctga
     2101 ccaagggcaa ggccatcatc accaccgagg tcggccagaa ccagatgtgg gcggcccaat
     2161 tctacaacta tgacgaacca aacacattcc tttcctccgg cggcctggga accatgggct
     2221 acggcttccc ggcggccctg ggggcgaaga tcgctttccc ggacaagcta gtcgtggata
     2281 tcgccggcga cggcagcatc cagatgaaca tccaggagat ggccacggcc atccaggaca
     2341 atatccccgt caaaatcgtc ttgttgaaca acggttatct gggcatggtc cgccagtggc
     2401 agcagctctt ctacggcaag cgctattccc acacggacat gacctatcag cccgattttc
     2461 tgaaactcgc cgaggcttac ggatggctgg gactccgggc gaccagaccc gacgaggtgg
     2521 aagcggttct gaagcagggt ctggaggccc ccgggccggc gctcatggac ttccggattt
     2581 gccgggagga atgcgtctat cccatggtgc gccccggcgg cccgattcat gaaatggccc
     2641 ttggccggga gatggcaaac tgaaatataa tttaacagga ggaaacattt agattatgga
     2701 gaagaaagaa tacactttgg cactgctcgt cagcaacaaa cctgatgtcc tggcccgggt
     2761 ggctggcatc ctgggcggcc ggggctacaa catcgagacc ctgtgcgtgg acgcgacttt
     2821 agatcccgcc tattccaaga ttatcctcac gacgatccag gataagacga cgataaccaa
     2881 gatcgaaaaa cagctcatca aactcatcga cgtccataag gtgacggacc tgaccaaggt
     2941 ggaatcggta acccgggaac tcatgctggt ccgtatgaaa ctctccgata cgaacaaggg
     3001 atacctggcc ctgatcatcg acaccttcaa cggccgggtg gtgacgatga acggcgacaa
     3061 ctgcgtcatc gagataaccg gcgatcggtc gctgatcgaa aaggccgcgg cgagcttgaa
     3121 acctctgggc attgatgata tggcccggac ggggatcatt tccctggaac gtcagaatta
     3181 aaggaataag gaggagacgg ttatggcaaa gttgaacatt ggcggggtca ttgaagatgt
     3241 gatcaccgcg gaggaattta ccctggacat ggcccgggaa gccctcaagg gcgagaccgt
     3301 ggccatcatc ggttatgggg ttcagggacg gggccaggca ctgaacatga aagacaacgg
     3361 catcaacgtc atcgtcggtg agggggacta caactggcag aaggcgatcg acgaaggttt
     3421 cgtccccggc aagaccctct ttccgcccat ggaggcggcg gcgcggggga cgattatcca
     3481 gtatctcctc tccgacgccc agcagaaggc catctggccg gaattgaagt cgcatctgac
     3541 caagggcaag gccctttatt tttctcacgg cttttccatc gtttacaagg aacagaccgg
     3601 tgtgatccca cccccggaca tcgacgtgat ccttgtcgcc cccaagggcg cgggaaggac
     3661 ggtgcgggac aacttccttg ccggtagcgg catcaattcc agttttgccg tcttccagga
     3721 cgccacgggg cgggcccgga aacgggccct ggccaccggc gtggccatcg gttccggcta
     3781 tctctttccc accaccttcg aaatggaggt ttacagcgac ctcaccggtg agcgtggcgt
     3841 cctcatgggc tgcctggccg gggtcctgga ggcccagtac aacgaactgc ggaaacgggg
     3901 tcacagcccc agcgaggcct tcaacgaaac cgtagaggag ctcacccaga gcctcatgcc
     3961 cttggtggcc cagaacggca tggactggat gtacgccaat tgcagcacca ccgcacagag
     4021 gggcgccctg gactggaaga atcgcttccg cgacgccgtc gccccggtct tcacggaact
     4081 ctacgaccgc gtggccgcgg ggaaagagac ggagatcgtc ctgaaggccg gtgccgccgc
     4141 caattatcgc gacacgctcc aaaaggagct ggatctgatg aagaacagcg agatgtggca
     4201 ggccggcgcc accctccggg cccttcgtcc ggagcgccgg aagaagtaga tccgggaaga
     4261 gaagataccc ccgtcaccgc tcttatgcaa ctgcttcgga agcaagcagg caatggcaac
     4321 ggcaatataa tcgcccatgt cgctccctcc ccttcaaggg gagggtcggg gtgggaatgg
     4381 acttacaaag gcataaataa atattcaaca attaaaagta tttatacaag gagggacaca
     4441 tgcgcagcga cttgatgaag aagggcctgg agcgggcgcc ccaccgctcc ctgttcaagg
     4501 ccatgggata tacgaacgaa gagatcgggc ggccgatcat cggcgtcgtc aactccgcca
     4561 acgaaatcat ccctggccac atccacctgc ggatcatcgc cgacgacgtc aaggcgggga
     4621 ttcggctcgc cggcgggacg ccggtggagt ttccggccat cggggtttgc gacgggatcg
     4681 ccatggggca cacggggatg aaatactctc tggccacccg ggaactcatc gccgattccg
     4741 tcgaatccat ggccatggcc caccccttcg acgccctggt gatgatcccc aactgtgaca
     4801 agatcatccc cgggatgctc atggccgccc tgcgcctgaa tatccccgca atcttcgtca
     4861 gcggtggccc catgctggcg ggcaagctca aaggcaagcc catcgacctc atcagcgtct
     4921 tcgagggcgt gggaaaggtc aaagccggga agatttccca aaaggaattg aacgccatcg
     4981 aagattgcgc ctgtcccggc tgcggctcgt gctccggaat gtacaccgcc aattccatga
     5041 actgcgtaac cgaggccctc ggcctcggcc tgccgggcaa cgggacggtc ctggccgtcc
     5101 acgctgcccg gcgccgcctg gccaaagagg cgggcatgaa gatcatgcag ctcctgaaga
     5161 aagacatcaa accccgggac atcgccaccc tcgccgcctt caagaacgcc atcgccgtgg
     5221 atatggccct gggctgctcc accaacacgg tcctccacat ccccgccatc gcccacgagg
     5281 cgggcatcga tctggatctc ggcctcttca acgagatcag caagaagacc ccgaacctct
     5341 gcaagctcag tccggcgggg gtgcatcata tcgaggactt cgacgcggcc ggcggcatcc
     5401 aggccctcat gaaacgcatc caccccctgg gcgtcatcga tgagcaggcc ctcaccgtca
     5461 ccggcaagcc cgtcggcgaa aacatcaaga tggcggaagt tctcgatgcc gaagtgatcc
     5521 ggtctctcga caatccctat atcaaggaag gcggtttggc catcctcaag ggaaatatcg
     5581 ccccggaagg ctgcgtcgtc aagcagtccg cggtggcgga aaacatgctg gtcaacgagg
     5641 gccgggcccg ggtcttcgat gcggaagacg acgccatcca ggccatcctc ggcggcaaga
     5701 tcaaggcggg ggacattgtg gtcatccgct atgaaggccc caaaggcggc ccggggatgc
     5761 gggagatgct gggacccacc tcggctatcg ccggcatggg cctcgacacc accgtggccc
     5821 tcctcaccga cggccgcttc agcggcggca cccgcggcgc cgccatcggg cacatctccc
     5881 cggaggcggc ggatggcggc cccatcgctc tggtccagga gggggacatc atcgccatcg
     5941 atatccccaa aaagaaaata accctcaagg ttacgccgga ggagttcaaa aaacggaaga
     6001 aggaatggaa gcccaaggca cccgccatca ctaccggcta cctggcccgc tacgccaaac
     6061 tggtgacatc ggccagcact ggggcggtct tcaaggactg acggcatatt cccttggtat
     6121 aaaccggaag cggggatgct catgacggcc tgttgttgcg gagagggatg aagccctcat
     6181 gacggtacgc cgtcattaag ggtgttgaaa cgtgcaagac tgtactccgt agcaaagggg
     6241 gatgaaagtg cgagacccat cccccttata acccccccct tgaaggggag gaaatcgttg
     6301 caagttaaat cgggcactta ggattgtatt ttccgataaa aggaggccga catcatgaaa
     6361 atgtttcttg tcgtttatgc ggactacttc gatgaacgca tcagcaaaac gttcatggaa
     6421 gccgggtttg gaaaatatac gaaagtgcat ggggttacgg gacggggcga ggagtcggag
     6481 gcaaagttgg gcacccatta ttcacccgga aaaaacaaca gcatattcat ggccgtgcag
     6541 gacgaggaga ttccccgtct tctcgaggtc attcggggcc tcagagcgga gtatcccggc
     6601 tccgggttcc gggcgtttac cctgccgctg gaggaatgca tctaacgata gtaagcggcg
     6661 tctattccag gaccattatg atcggcgacg gtacacgttt cccgggacct tattatacag
     6721 gctgaaaccc taacctccat agcaagcagt catgagggag ggggaccggc agggcgatca
     6781 gagctgccgg tcgaatggcg cgggaggagg tcgtgacgat aaaggaaacg aatatggccg
     6841 gccaccctgc cgatcctgtc gctccccccg gtgcagtgtt catggatgtt tccgtttgcg
     6901 tagaaaggga cgtgagcatc cgggttatga cctggcggcc ccaacgaccc gtcagtgagg
     6961 cacccataat cttcgtagcc ggctgggtat ccgccgtatc gggatgggcg cccctgttga
     7021 aggtcatggc cgccgtcagg cccgtttatt atatcgaaac acgagaaaag agatcggccc
     7081 gcatcgagaa gaaggacctg caaccggagg atttcagcat cggccggatt gcggcggacc
     7141 tgatcagcgt tagtcgccaa ctggagattg ccgtgtcaaa caccaccgtc atcggcagct
     7201 ccctgggggc aacggccctg ctggaggccc tgaagggggg agaactgacc gcgggggccg
     7261 ccttcctgat cgcccccaac agcgacttcc cggcgccctg gcatatccgc tggatgctta
     7321 cgctgccggc attcctgtat cacggactca agcatatcgt cctttggtac gtgaggaact
     7381 tcatggtcga tgtgaagaac gaaccggaac agatgcagcg ttacacggaa acgctgcagg
     7441 ccgcccatcc ccagcggatc aaactatcgg cccaggcggc catcgacggc cgctacgagg
     7501 tctggccgca tctggaaacg gtgcggatac ccgttgccct tgcttacgcc cccacggaca
     7561 agttgcactc cgcaaccaat atccgtctca tagcggcgcg gcttccccgg gcagaaatca
     7621 tcgtctgtcc ctccaacagg tatatgcacc gcccggaatt gatggccgat atcgaggcgt
     7681 ttatgaggat gacatattat agtcaatgca gtcatcaagg tccgttctac cgtgattcca
     7741 tgatggggca acggatcatt cgcggtcccg ccgaagatga cgggttgaca aaagacgaca
     7801 aatcatcgta ggttgtaagg gtaatgattt ccacacatct ggacaatttc accgtcgtcc
     7861 tccataaacc caagtatccg ggcaacatcg gctccacggc acgctgtctg aagaatatgg
     7921 gaatgggcaa actcatcatc tccgaagcgg gccctttctc cccagcggag atgaagatca
     7981 tgtccaccca tttcgccgcg gacatcgtgg atcggatcgg ctatcatgat cgcctcggcg
     8041 acgccctcgc cccgtttcaa tacattgtcg gcactacctc ccggctcggg tccgcccggg
     8101 gtcccgtcct ctccccgcgg gagatatcgc cttccctggc ggagttatcc cggcgcaatg
     8161 aggtggccct cctcttcggc gccgaagaca aaggcttgag caacagcgat cttcgttatt
     8221 gtcactccgt cgtggccatc ccggtgtcgg aaaaactcaa atccatcaat ctttctcatg
     8281 cggtgatgat tctctgttac gagatatccc tcgccgcagc agagagaaaa agtgttttca
     8341 ccccaaagct cgcctcttcc cgggaaatcg aggggatgta cgaacaggtc aaggaggtgt
     8401 tgatggaaat cggcttcctc aatcctcaga atccggatta ctggctcatg cacatccggc
     8461 ggctgctgtc ccggacggga cttttttccc gggacgtcaa gatcatccgg ggcatctgcc
     8521 gccaggtcag gtgggcgtta cggaacaaaa attcttgaca ttttcacgcc ggggttgcag
     8581 agaataccgc ggtaaaggtg actgtcgagc aaaagcgacc ggatcaagct tcgatagaga
     8641 gaatgccatg attatcctta aaaaattgtt tctgtttatg attgccttat ttttacttcc
     8701 cggtttgacg gggtgctggt atgcagcggc ggccggcgct ggcgcctatg ggggttacaa
     8761 ggcaaaagag gcgggctact ccctccaaaa cccggttacc aagaaagatt ccagcaagaa
     8821 aagtagccca aaagaaacta aaaagcagac gaccactcag caatgatctt tcctgtccca
     8881 cacgggggca aagaaagacg aatatattcc atgataggag ggaatcatgt cgaaggaaga
     8941 aaagatcgca gccatcaagc aacgggccag gaagaacttt accctgggtt acaattgtgc
     9001 ggagtgcatc gtcgaagcgg tgaccggcga aatcgacacc ggcctgccac cggaagtgaa
     9061 aaaggtggcc acggggttcg gcggcggtgt cggcttatac ggcgacacct gcggcgccat
     9121 cactggggcg attatcgccg taggctgtgt ccatggccgc ggcggtctgc cggaaggaga
     9181 gggcaaagag gtggtaaaaa aggcggtaaa ccagctctac ggaaagcccg gggcctaccg
     9241 tctcttcaat cagatcccga tcaagttctc cgataagtac ggtaacaccc tgtgtcggga
     9301 attgacctcg aaatggcatg acagttggct ctgccgggac catgccctgc actgccggga
     9361 gatcatcacc gatgcggcgg aaatcgccgc ggagctttgc ctggctgatt ataacgaggc
     9421 agcctccaag cccttcggaa aaaacgtgga gaatctcaag gatcccgccg ccgatcagtc
     9481 cgacaaaccc acttgaggca ccgcaggtgg agcaggttgc tccaactaag aactaacgca
     9541 ccattcgcac gctggaaccg tagcgtcgat aaagtgaaaa tagaacggga aaattccatt
     9601 gtcaaccggc catcccagac gggatggccg gttgacccct tgaatgaatc tcctcgcagc
     9661 aagctgcgag gtatcaaaac ttcaaggaac gaagcaagct tcggggaatt caccctgaga
     9721 gattaaataa atgatcacct ttcgaccgta atttccacat ggaaaaaggg gggccgatta
     9781 tgatcacgcc tgggggctat cttacccgca tccctttttc cctccggtta tgactctttg
     9841 gttttgacct tgaccgaaat actccggcag cattcggcat ttttcacgac aaaacagtcc
     9901 ggtcgttcca ggaactgata ctggccggtg gattcgatct ccagatcgtt ccgggtcaga
     9961 ttgccttcct tggggatgaa caacatgcgg ccgatgggta tccccgcttc cttcaagatt
    10021 gccattgccc gattctcctc cttatttatg ttaaaaccct tttgtacaaa tgaaagtccc
    10081 gtttcggcgg cccggttcat caacaatcgg cggtcttctt ttttttgacc cggccccacg
    10141 gcctccaccg ccacgccatc cggtattgcc gccgttattc aataacacct ttgttgaccg
    10201 ttgctcattg ctccgctgcc aataccgact ttacctcaaa aggatcaagc ccgtccaggt
    10261 cacccgtcct tggacgggaa aggcaaggga caagcctacg ttctttagtg tagccaccac
    10321 gtcgatcccc atggcctcca gggaagggcg ggcctgaaag ggaaaacggc aatgcatccc
    10381 gccttccgcg gccacgcaaa ccggacagag acggcagcag ccgccgatca acccggcggc
    10441 gaagcgaaaa ccggtcagaa aggcctcctt ttctccccgg ttgaccagtt cgtgaagcct
    10501 tcccgccggg gcatacacat cctcatgggg ccggcagccg tccaggccag caatctcttt
    10561 cgccacttgg aggacaacgg cctcctggta acggctcaga gcttcccgga attccgtcac
    10621 ggacatgaca aaggggggac acatgaggtt cttgtgaaaa ctgtcgcaga tgggcacccg
    10681 gcacttcagt cggacccgct cgtcaacgac gatccggtcc accgccagag gcagggcggc
    10741 cgtcgccccc tgttctttga ggaatgcgat tagggaagcg atcttttgcg tcgcgtcgct
    10801 ggtgttctgc atcttcccag ttattaggca tctcaaccga cgccgctgtc ggatgaacgt
    10861 ctttcactcc gatgacgccc cggatttcgg cgtctccgga aaggtcagca cggccgggat
    10921 ggatgcccgt taaggttcac tgcgaacccg ctctcaaatc accgaaggtg attcttgttc
    10981 aaaaatcacc gctttccccc ggaattaaag agtggcgggg cttaaaactc catcccggcc
    11041 cgttccttga tccccttggc gtagtgatgt tttatctcct gaatctcgct taccgtatcg
    11101 gccagagcga caatctccgg gggggcgtac cgtcccgtga ggatgagatc caccgccggc
    11161 ggtttattgg ccaccacggc gatgacgtcc tccagattga tcaacttgaa atccagggcg
    11221 acgttgatct cgtcgaggat gaccagttca tgctccccgg cggcaaccac ctgccgggcc
    11281 atctccagtc ccgcctgagc cagttcccgg tcgatgggcg ccgggttatc ccgcatgacg
    11341 aaactgtcca gaccgctggg gcggatggtg aattcgggca gacaacgctc ggcggccagg
    11401 acttcgccgt actttttgcc cttcatgaac tggatcatga aaaccttgcg cccatgaccc
    11461 agggcgcgga gagcctgtcc tacggcggcc gtcgtctttc ccttaccatt gccggtaatg
    11521 accattacaa tgccccggtt gaactttgcc agggtcgctt cgattatatt gtccgccgcc
    11581 actgtctctc ccctatcctg atcggacgat gattgtggtc cagatgaaag cccgggatga
    11641 ggttcatcat cccgggcttt taccgccgct caaaacaaat ttaaccactg cattccttga
    11701 gataacgtcg cgccacatct tcggcgaggg tgtagggatc cgtctccttg ttgatcagtt
    11761 tctggatcat cccttcgatc tcgccgttct gcacgaggcg gttcagaaca ggctccagga
    11821 cgctgttccg gatcgcctcg ttgagctcca ccaccgcctt ccggcggacc cgggatccga
    11881 tgagattgtg ctgaacgaga tgctggtagt gtttttctat cgtcgccgac agttcctcca
    11941 ggctcttgcc gaaaccatcc ggttcgaagg cgttttccac cctgatgatg ggggggcgcc
    12001 agcccgccgc aaaggtctcc ggcggtgcca tgtcgagcat ggccatgatc tcccgccgca
    12061 gcttggagga gccttcccgg tcggccttgt tgataacgaa gatgtcggca atctccatga
    12121 taccggcctt gatggcctgg atctcgtcgc ccatccccgg aatcaggacc acgaggaccg
    12181 tatgggaatg gttgatgatg tccacttcct gctggcccgt gccgacagtc tccacgatca
    12241 ccttgtcttt acccatgacg tcgaggatgt gaatggcgtc gcccacggcc ttggccagcc
    12301 cccccagggc cccccgggtc gccagggagc ggacgaaaac ccccggatcc tccgcatggc
    12361 gctgcatgcg gatccggtcg cccaggatcg ccccgccggt gaaagggctg gtcggatcca
    12421 cggccaggac gcccacggtt ttttccttct tacggaagga ctccaccagg ccatcgacga
    12481 gggtgctctt ccccgccccc ggcgaaccgg tcagaccgat gacatgagcc cggccggtta
    12541 ggggaaagat gtgtttgatg gtggatcgcg cttcgatcat cccatcttcc aggttacgta
    12601 tgagacggga tgccgttcgg acgtccccgc ctctaatttt tttcactttt tccatgatat
    12661 caattccttg gctgtacatt cgttctgaac caatccacga tgctgtccag ggtcgcgccg
    12721 ggtacccaga tcgccttgag accggcgtca tatagcatct ggaaatcctg ttccggaata
    12781 atcccaccgc ccacgacggt gatgtcgtcg gcgccttgct ccttcaggag cttggcgacg
    12841 gcggggaaca gataacgatg ggccccggac aggcagctca gccccactac gtccacatct
    12901 tcctggatcg ccgcggaagc gatctgttcc ggcgtctggt gacagccggt ataaatgacc
    12961 tcgaagccgg cgtcacggaa acagcgggcc aggaccctgg cgccgcgat
//
LOCUS       DAOSMF010000045        12882 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_5653_length_12882_cov_21.446168, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000045 DAOSMF010000000
VERSION     DAOSMF010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 12882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12882
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5653_length_12882_cov_21.446168"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(503..1318)
                     /gene="fdhD"
                     /locus_tag="PLT63_06110"
     CDS             complement(503..1318)
                     /gene="fdhD"
                     /locus_tag="PLT63_06110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HOD98332.1"
                     /translation="MSVDERLYETFPVQEINSTGGVVRLADMEKSVIGEISLKIFING
                     TEYASLLCLNQFAPELAVGFLYSEGVIDTMADVASLTYNDRLFAVIIELVPGKSVEKS
                     ESLRSVTSGCGKCFTYINPIKNEKYQTIDCQRRYSLSHILLSMKRFERQSVVYRRVGG
                     VHSVLFHHHSFGVFIEDIGRHNCFDKITGILLTNDKMQLVRDSVFFVSGRVSSEIITK
                     VIRLGVPVLVSRSTPTAAAVNLAAKYGVTLLGYVRNGSGYVYSGKNRLIEGDI"
     gene            complement(1318..2274)
                     /locus_tag="PLT63_06115"
     CDS             complement(1318..2274)
                     /locus_tag="PLT63_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HOD98333.1"
                     /translation="MKFDHDVLPAKTGEEINLAEVVDALCVANNGFSGALRRDQREQP
                     LPSRDIIIEIVEALRSVLFPGYFGFSGLKASSVHFHVGATLDRIRPVLEEQIKRGRCF
                     SCREDRENGCLPFCDERSAEIVNRFFARLPAIQRLLATDVQAAFEGDPAAASPDEVIF
                     CYPGLQAITNYRLAHELRLLEVPLISRMITEYAHGITGIDIHPGATIGRYFFIDHGTG
                     VVIGETCIIGDRVRIYQGVTLGAKSFKLDAEGKPIKGVERHPVVEDEVIIYSGATILG
                     RVTIGRGSVIGGNVWLVESVPPYSHISQAQTHQELYEHGAGI"
     gene            complement(2271..2732)
                     /locus_tag="PLT63_06120"
     CDS             complement(2271..2732)
                     /locus_tag="PLT63_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HOD98334.1"
                     /translation="MTEGMVIAVCRSDRKGTRKTVVPAGVFITDFGLAGDAHAEGGIN
                     RQVSLLAVSSIEKMKKLGIEVGSGDFAENLTVSGLALHYLPPGTQLQAGAEVILEITQ
                     IGKACHKGCAIFAQVGTCIMPKEGVFARVVKGGTIFAGDRIEVIREVEGSS"
     gene            complement(2890..4101)
                     /locus_tag="PLT63_06125"
     CDS             complement(2890..4101)
                     /locus_tag="PLT63_06125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HOD98335.1"
                     /translation="MAKIPLKSCPKVYILETHRSRTPDSTLCFVEKVKDTAGMLSFRN
                     ATEVDRIGISVFTCDRMRPDGSKTSHTGKGVSPIQAQVSITMEAIERYCSEYREEYGG
                     KLLWGSFSALGKKFNILDPREMILSRSGDYSHEKEISWVWGHDLASDKEILVPACAVY
                     HPFPQHGTFLFNTHTNGIAAGNTYEEAVIHGLAELIERDAWSIAKYTHAYNDALFMEN
                     EPGNEFIIDVLERFEKADIEIVTKDLTTDIDVPVIAAFSRDLMYREMVPIDGFGAHLD
                     PRVALVRALLEIATTRALFLQKFGLSGLCDTSPLYLQGEGYEDDPRFYAYRRKGLSEL
                     DRGYSDDIREDVEEMIARLSAAGLERVIAIDLQREDIGMPTVRMIVPGLETYCFDTTR
                     AGKRAERFLEG"
     gene            complement(4171..5079)
                     /gene="nadA"
                     /locus_tag="PLT63_06130"
     CDS             complement(4171..5079)
                     /gene="nadA"
                     /locus_tag="PLT63_06130"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOD98336.1"
                     /translation="MSIAERIQQLKKERNAVILAHNYQIPAVQDIADFVGDSLGLSIQ
                     ASKTDAEVIVFCGVHFMAETAKILSPEKTVLLPEKAAGCLMADMIDADQLRALKAEHP
                     GAKVLCYVNTSAAVKAECDLCCTSANAVRMVREGLREAAEIIFVPDKYLADYVSAQTG
                     RSFIVWPGFCPTHARILPENVREEKVLHPEAVVIAHPECPPELRQLADCVTSTEGMCR
                     YVRESTAREIIVATERGILHRLRKENPGKIFYPASRFALCMNMKLIQQEKILWALEDM
                     GHEIVVPPEVAEKARAGIQRMLDFSE"
     gene            complement(5107..6168)
                     /gene="mnmA"
                     /locus_tag="PLT63_06135"
     CDS             complement(5107..6168)
                     /gene="mnmA"
                     /locus_tag="PLT63_06135"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOD98337.1"
                     /translation="MSKKVALALSGGVDSSTAAWLLKEQGYDVVGVTMCLGIPVAEGE
                     RGKCCGPQEIEDARRVCQKLAIPHYVLDFADELDSMVIQPFVSAYLQGRTPNPCVECN
                     RSLKFGSLLHKVRALGFDCLATGHYARVEHTATYSLLRKPRDEKKDQTYFLYAMGKEE
                     LRRVLFPLAEYSKAEVRKLAGWAGLPIAEKPDSQDICFIPADGLASFLHRRRPEGFSA
                     GDIVDRTGRVIGRHRGLPFYTVGQRGGLGISHRVPLYVLALDVPGNRLLVGEKEEVRA
                     MGLIAEHLNLLVERPPDRATAKIRYAHRAAPCRIAWKKERAAIHFDTPQEAVTPGQSV
                     VVYDGDLVVGGGIIRGAIS"
     gene            complement(6168..6551)
                     /gene="nifU"
                     /locus_tag="PLT63_06140"
     CDS             complement(6168..6551)
                     /gene="nifU"
                     /locus_tag="PLT63_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HOD98338.1"
                     /translation="MGSQYSEKVMDHFANPRNVGVIENADGVGKVGNSVCGDIMNLYL
                     RVENDIITDAKFKTFGCGAAIATSSMVTELVKGKTVTEALKISNRAVAEALGGLPPIK
                     MHCSVLAEDALQAAIDDYLKRRREG"
     gene            complement(6562..7731)
                     /gene="nifS"
                     /locus_tag="PLT63_06145"
     CDS             complement(6562..7731)
                     /gene="nifS"
                     /locus_tag="PLT63_06145"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HOD98339.1"
                     /translation="MERIYLDYAATTPCASEVVAAMLPFFSDRFGNPASMHAFGQENK
                     GAVEEARERIASFLGAAPGEIVFTSGGTESNNTAVKGVAYARQGKGNHIITSPIEHHA
                     VLEPCHFLEKEGFEITILPVDGDGLVDPDDVRKTITGKTILISVMHANNEIGTIQPIE
                     EIGRIARERGVYFHTDAVQTLGHLPFTVGELHVDLLSASAHKLYGPKGVGILFVKKGT
                     RLTPFMHGGEQENRRRASTHNVPGIVGFAKAVELASEGMETEVRELTALRDRLITGLR
                     EGIEAVKLNGHSTRRLPNNVNISVAHVEGEGLLLSLDMLGIACSTGSACSSASLEPSH
                     VLMALGLPHELAHGSLRFSLGRPTTAADIDRLLAVLPPVVQRLRAMSPLYNQQNK"
     gene            complement(7734..8354)
                     /gene="metW"
                     /locus_tag="PLT63_06150"
     CDS             complement(7734..8354)
                     /gene="metW"
                     /locus_tag="PLT63_06150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HOD98340.1"
                     /translation="MNMERNDTPERADHRVIYEAIPPESRVLDLGCGTGELLSHLVRG
                     KHARVQGIEMDGRSVYECVKKGLSVFQGDIETGLTEYPDGAFDDVILNQCLQEVRKVD
                     FVIAESLRVGKRVIVGFPNFAYFRARIMLGLGGRSPVTASLPYLWYDTPNVRFLSIRD
                     FRIFCLDKGLHVLTSHFIGANGPVTFWPNLFALNAVFVLAKRKEGK"
     gene            complement(8351..9544)
                     /locus_tag="PLT63_06155"
     CDS             complement(8351..9544)
                     /locus_tag="PLT63_06155"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HOD98341.1"
                     /translation="MVSTALKKNNFQNDIGTVETKYYTFAHPPNELVLECGERLGPIT
                     LAYETYGRLDARRSNAILILHALSGDAHAAGVHGEDGATGWWEEMIGPGKGFDTNKYF
                     VICSNIIGGCKGSTGPAAINPTTGRPYALGFPFITIADMVNTQRRLIDHLGIDRLLCV
                     AGGSMGGMQVLQWAASYPERVRAAIPIATALKHSPQQIAFNEVVRQSIMADPAWRKGD
                     YYDHGQPEKGLAVARMIGHITFMSDRSMEEKFSRRLKNGVFSFNFDADFEVEGYLRYR
                     GANFVKRFDANSYLYITKAMDYLDLSAGRLIRQDKLIATRFLVISFSSDWLYPSYQSQ
                     EIVRELKGRQVDVAYCELKSTYGHDAFLVETAEQTELIRNFLRVTQQGYHSWPGRGTT
                     VGGFA"
     gene            complement(9748..10185)
                     /locus_tag="PLT63_06160"
     CDS             complement(9748..10185)
                     /locus_tag="PLT63_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOD98342.1"
                     /translation="MRLSTRGRYGVRFMFDLASHYGEGPIFLKDIAQRQGISEKYLWH
                     LVPPLKSAGLITTVRGAKGGYYLSRPPQSINLMDVVRTLEGPICFVDCVENPSCCDRA
                     EVCISREVWGEASQKVGEILMGITLENLVQRQRTKVPDTGMFI"
     gene            10561..11859
                     /locus_tag="PLT63_06165"
     CDS             10561..11859
                     /locus_tag="PLT63_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HOD98343.1"
                     /translation="MTLRRLETLALHSGQESPDPATGARAVPIYQTTSYVFQSTEHAA
                     SLFALQQFGNIYTRMTNPTTEVFEQRIAALEGGTGALATASGQAAITLAMLNVTRLGD
                     EIVAADNLYGGTYQLFHYTLPKLGRTVKFVDSRQPEQFRRAITNKTRAVYAETIGNPK
                     LDVPDFEAIAKIAHAAGIPFIVDNTVGVGLVRPIDYGADILATSATKFIGGHGTSIGG
                     VIVDGGKFDWGNGKFPEFTAPDPSYHGLRFWDAFRAVPGLGNVTFIIKARVQLLRDIG
                     ASLSPFNAFLFLQGLETLYVRQRRHGENALAVARFLKDHRAVNWVNYPGLEDHPSHAL
                     AVKYLKGNYGALVGFGIKGGLEAGKKFINSVRLLSHLANIGDAKSLVIHPASTTHQQL
                     TRTEREATGVTEDFIRLSIGIEDVEDIKEDIDQALRQAAA"
     gene            11887..12810
                     /gene="cysK"
                     /locus_tag="PLT63_06170"
     CDS             11887..12810
                     /gene="cysK"
                     /locus_tag="PLT63_06170"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: protein motif:HMM:TIGR01136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOD98344.1"
                     /translation="MKIFADITKTVGGTPLVELRRLSAGARARIVVKVESFNPLSSVK
                     DRIGVAMIDAAEKSGLLQSGSVLIEPTSGNTGIALAFVASARGYRLILTMPDTMSLER
                     RQLLEIFGAELVLTPGGEGMSGAVCKAEELAGAIPGAVMLQQFQNPANPEIHRKTTAE
                     EIWNDTDGGVDILVAGVGTGGTITGIAEVIKERKKGFQAIAVEPESSPVLSGGKPGPH
                     KIQGIGAGFIPRVLNRGIIDEIVKVRNENAGAIARRLAREEGMLVGISGGAALWAALE
                     VAKRQENQGKLMVVILPDTGERYLSTWLFKE"
BASE COUNT         2628 a         3833 c         3765 g         2656 t
ORIGIN      
        1 ggggcagatc ccctacgaag ccatggacaa gggcgccctg ataatcaaac agttcgccgc
       61 ggggaactac gtgacttcct tcatgatctc cggtgtcatg tgcctggcct ccatcggcat
      121 tgtctggatg ctcaagccgc cgacgaagtg agggcgtaac ggcgtacaag cggtttaacc
      181 gtatttgacg gatattcccc cgccgccccc ccctccccgg catggggacg gaaggagtgg
      241 gggggctgaa gagggccgac gccttgccgg tcccgggcag ttgacgatag aatagacgca
      301 tcggaagggc cgtcccgacg gaaacgggac ggcccctttt ttttcaggga cgatgggggc
      361 gaggagtgct accgggaatc agccggtcgc atccgccgag gccgctgcgg gtacggcggc
      421 cttccggcgc cgggggcggc gtctccggct ccttttcttt gtatggttgc ttccccggcc
      481 ggggcatcgg catcggaaca tatcaaatat caccttctat caaacggttt ttgccagaat
      541 atacatagcc ggaaccgttt cgcacatacc ccagcagggt aaccccatat ttagcggcaa
      601 ggttgaccgc cgccgccgtg ggcgtggagc gggagacgag tacggggacg ccgagccgga
      661 tgaccttggt tatgatctcg gaagaaaccc gcccgctgac aaaaaacaca ctgtccctca
      721 ccagttgcat tttatcgttc gtgaggagga tgccggtgat cttatcgaaa cagttatgtc
      781 ggccgatatc ctcaatgaag acgccaaagg agtgatgatg aaataagacg ctgtggaccc
      841 cgcccaccct ccggtaaacc acggactgcc gctcgaatct cttcatggaa agtaggatgt
      901 gggagagtga gtacctcctc tggcagtcga tggtttgata cttttcgttt tttatgggat
      961 tgatgtaggt gaagcactta ccgcatcccg aagttacact gcgcagactt tccgactttt
     1021 cgacggattt gccgggaacc agttcgatta ttaccgcgaa gagacggtca ttgtaggtca
     1081 gggaggccac atcggccatt gtgtcgatca caccctcact gtagaggaat cccaccgcca
     1141 gttctggtgc gaattggttg aggcagagaa gggaagcgta ttcagtgccg ttgatgaata
     1201 ttttcagtga tatttcaccg atgacgctct tttccatgtc cgccagccgc acgaccccgc
     1261 cggtgctatt gatctcctgg acggggaatg tttcgtatag cctttcgtcc acactcatca
     1321 gatccctgct ccatgttcat aaagctcctg atgggtctgg gcttgagaga tgtgactgta
     1381 gggcgggacg ctctccacca gccagacgtt gccgccgatg actgatccgc gaccaatggt
     1441 gacccgaccg aggatcgtcg ctccggagta gatgatcact tcgtcctcca ctacgggatg
     1501 cctttcgacg cctttgatcg gtttcccctc cgcatccagt ttgaagcttt tcgctcccag
     1561 cgtcaccccc tggtagatcc ggacccggtc gccgataatg catgtttcgc cgattacgac
     1621 cccggtgccg tggtcgatga aaaaataccg gccgatcgtc gcccccgggt ggatatcgat
     1681 ccccgtgatg ccgtgggcgt attccgtgat catccgggag atgaggggaa cctccagaag
     1741 ccgcagttca tgggccaggc ggtaattggt gatcgcctgc aacccggggt agcagaagat
     1801 cacctcatcg gggctggcag ccgccgggtc gccctcgaag gccgcctgaa catccgtggc
     1861 caggaggcgc tggatggctg gcagccgggc aaagaagcga ttgacgattt cagcggaacg
     1921 ctcgtcgcaa aaaggtaagc aaccgttttc ccggtcttcc cggcaggaaa agcaacgccc
     1981 ccgttttatc tgctcttcca gaaccggacg gatgcggtcc agcgtcgcgc cgacgtgaaa
     2041 gtggacgctg ctggccttga gaccggagaa tccgaaatac ccgggaaaaa ggacggagcg
     2101 taaggcctcg acgatctcga tgattatgtc ccgggagggg aggggctgtt cacgctgatc
     2161 ccggcgcagg gccccggaaa atccgttgtt ggcaacgcat agggcatcca cgacctccgc
     2221 caggtttatt tcctctcctg tctttgccgg cagtacatca tgatcaaatt tcatgatgat
     2281 ccttccacct cccggataac ctcgatccta tcaccggcaa aaatggtgcc gcctttgacc
     2341 acacgagcaa agaccccctc tttgggcatg atgcaagtcc ccacctgggc aaagatggcg
     2401 cagcccttgt ggcaggcctt accgatctgg gtgatctcca ggatgacctc ggcgccggcc
     2461 tgtagttgcg ttcccggcgg caggtagtgc agcgccaaac ccgagacggt gagattctcg
     2521 gcaaagtctc ctgaccccac ctcaatgcca agcttcttca tcttttcgat gctgctaaca
     2581 gcaaggaggc tcacctgacg gttgatgccc ccctcggcat gggcgtcgcc ggcgagcccg
     2641 aaatccgtga tgaatacccc cgccggcacc accgttttgc gcgtcccctt ccggtcactc
     2701 cggcaaaccg cgatcaccat accttccgtc attgcatctc ctcattaacc gtcatgcccg
     2761 ttccgggcgc aacgaaagat gaaaatatcc ggctagttgt ggacattttc ctataaattc
     2821 aattaaatat atttattatc cgtgtcatgc cccgatcgag aggaacggga tcagccgttg
     2881 ccacgggacc tatccctcca ggaacctctc cgccctcttt ccggccctcg ttgtatcgaa
     2941 gcagtatgtt tccagacccg ggacgatcat ccgcaccgtg ggcatgccga tgtcttctct
     3001 ttgcagatcg atggcaatta cccgctccag accggcggcc gaaagcctgg cgatcatttc
     3061 ctcgacatcc tcccggatgt cgtcgctata accccgatca agctcgctta gacctttccg
     3121 gcggtaggcg taaaagcggg gatcatcctc gtatccctct ccctggagat acagcgggct
     3181 cgtatcgcac aggccggaaa gcccgaattt ctggagaaat agcgccctgg tcgtggcgat
     3241 ctccagcagg gcccgcacca aggcaacgcg gggatccaga tgggcaccga aaccgtcgat
     3301 gggaaccatc tcccggtaca tcagatcacg ggagaaggcg gcgatgacgg gaacgtcgat
     3361 atcggtagta agatccttcg tcacgatttc gatatccgcc ttttcaaacc gttccaggac
     3421 atcgataatg aattcattcc ccggttcatt ctccataaac agggcatcat tataggcatg
     3481 ggtgtatttg gcgatgctcc aggcgtctct ttcgataagc tccgccaaac cgtgaatgac
     3541 cgcttcttca taggtgttcc ccgccgcgat cccgttggtg tgggtgttga acagaaacgt
     3601 gccgtgctgc gggaagggat gataaacggc gcaggcgggc acgaggatct ccttgtcgct
     3661 tgccaggtca tgcccccata cccatgatat ttccttttcg tggctgtaat cgccgctccg
     3721 ggagagaatc atctcccggg gatcaagaat gttgaacttc tttcccaggg cggaaaaact
     3781 gccccacagt aattttccgc cgtattcctc ccgatattcc gagcagtagc gttctatggc
     3841 ctccatggtg atcgacacct gggcctgaat gggggaaacg cccttgccgg tgtggctggt
     3901 cttcgagccg tccggcctca tccggtcgca ggtgaaaacg gaaatgccga tccggtccac
     3961 ctccgtggcg tttcgaaaag acagcattcc cgccgtatcc ttgacctttt caacgaagca
     4021 aagggtcgaa tccggtgtcc gggagcgatg cgtttccagg atatacacct tggggcatga
     4081 tttgaggggt atcttcgcca tatcgtcttt ctcctttata tgaaaaagtt ctcagtattt
     4141 gaacacttac atcacggaat agtatgggcg tcactcactg aagtcgagca tccgttggat
     4201 gccggcccgg gccttctcgg caacctccgg aggcacgaca atttcatgtc ccatgtcctc
     4261 cagcgcccag agaatctttt cctgctggat cagcttcatg ttcatacaga gggcaaacct
     4321 ggaggcgggg taaaagatct tgccgggatt ctccttccgc aaacggtgga ggatgccccg
     4381 ctccgtggcc acgatgatct cccgggcggt gctttcccta acgtaacgac acatcccttc
     4441 ggtagaggtg acgcagtcgg cgagctgccg caattccggg ggacactcgg gatgggcgat
     4501 cactaccgcc tccgggtgca gcaccttttc ttcccggaca ttctccggca ggatgcgggc
     4561 atgggtgggg cagaagcccg gccagacgat gaaggagcgt cccgtctggg cggatacgta
     4621 atcggccagg tacttgtcgg gaacgaagat gatctctgcc gcttcccgga ggccttcccg
     4681 gaccatccgg acggcgttgg cggaggtgca gcacaggtcg cactccgcct tgacggcggc
     4741 ggaggtgttg acgtagcaga ggaccttggc cccggggtgc tccgccttca gggcccggag
     4801 ctggtcggca tcgatcatgt ccgccatgag gcaacccgcc gccttttccg gcaggaggac
     4861 cgtcttttca ggagacagga tcttggccgt ctccgccatg aaatggacgc cgcaaaaaac
     4921 gatgacctcc gcgtccgtct tggacgcctg gatgctcaag cccagggaat cgcccacgaa
     4981 gtcggcgatg tcctgcaccg cggggatctg gtagttgtgg gcgagaatga cggcattcct
     5041 ttccttcttc agttgctgta tcctctcggc tatgctcatt ggtcgctcct tatccttcct
     5101 tatctattag ctgatagccc cgcgaattat ccccccgccg acaacgagat cgccgtcata
     5161 tacgaccacc gattgccccg gggtgacggc ctcctggggg gtatcgaagt ggatggcggc
     5221 cctttccttt ttccaggcga tgcggcaggg cgccgcccgg tgggcgtagc ggatcttggc
     5281 cgtcgcccgg tcgggcggcc gttccacgag gagattgaga tgctccgcga tgaggcccat
     5341 tgcccggacc tcttcctttt ccccgacgag caggcggttg ccggggacat ccagggccag
     5401 gacgtaaagg ggaacccggt ggctgatccc caggccgccc cgctggccga cggtatagaa
     5461 cggcagcccc cgatggcgtc cgataacccg gccggtccgg tccacgatgt caccggccga
     5521 aaagccttcc ggccgccggc ggtgcagaaa agaggccaga ccgtcggccg ggataaaaca
     5581 gatgtcctgg ctgtccggtt tttccgcaat gggcagcccg gcccacccgg cgagttttcg
     5641 cacctccgcc ttgctgtact ccgccagggg gaaaaggacc cgacgaagct cctcctttcc
     5701 catggcgtag agaaaatagg tctgatcctt tttttcgtcc cggggtttgc gcaggaggga
     5761 gtaagtagcg gtgtgctcca cccgggcata gtgacccgtg gccaggcaat cgaaacccag
     5821 ggcgcgtacc ttgtgcagga ggcttccaaa cttcagggaa cggttgcact ccacgcaggg
     5881 gttgggggtt cgcccctgga ggtaggcact gacgaagggt tggatgacca tgctgtccag
     5941 ttcgtctgca aaatcgagga catagtgagg aattgccagc ttttggcaga cccggcgggc
     6001 atcctcaatc tcctggggac cgcagcattt gccgcgttcc ccctccgcaa cgggaatgcc
     6061 caggcacatg gtgacgccga caacgtcgta accctgctcc ttcagaagcc aggcggcggt
     6121 ggaggaatcc acgccgccgc tcagggccag ggcgaccttt ttgctcatca cccctcccgc
     6181 cgtctcttca ggtaatcgtc gatagccgcc tggagggcat cctcagccaa tacggaacaa
     6241 tgcatcttga tcgggggaag ccctcccagg gcctccgcca ccgcccggtt ggagattttc
     6301 agggcctcgg tgacggtttt gcccttgacg agttccgtca ccatggaaga ggtggcaatc
     6361 gccgccccgc aaccgaaggt cttgaatttg gcatcggtga tgatgtcgtt ttccacccgc
     6421 agatagagat tcatgatatc gccgcaaacg gagtttccca ccttgccgac gccgtcggcg
     6481 ttttcaatca cacccacgtt gcgcggattg gcgaaatgat ccattacctt ttcactgtat
     6541 tgactaccca tgataacctc actatttgtt ttgctgattg tatagcggcg acatggcgcg
     6601 cagtcgctgt accaccgggg gcaacaccgc caggaggcgg tcgatgtccg ccgccgttgt
     6661 cggccggccc agactgaagc ggagggaacc gtgagccagt tcatggggca ggccgagggc
     6721 catgaggacg tgggacggct ccaggctcgc cgaggaacaa gccgagccgg tggaacaggc
     6781 gatgcccagc atgtcgagac tgaggagcag tccctccccc tccacatggg ccacggagat
     6841 attgacgttg ttgggcaggc gccgggtgga atgaccgttg agctttaccg cttcgatgcc
     6901 ttcccggagg cccgtgatca ggcggtcccg cagcgcggtc agttcccgca cctccgtttc
     6961 cataccttcc gaggccagct ccacggcctt ggcgaagccc acgatgcccg gcacgttatg
     7021 cgtcgaggcc cggcgccggt tctcctgctc cccgccgtgc atgaacgggg tgaggcgggt
     7081 tccttttttc acgaacagga tccccacgcc cttggggccg tagagcttat gcgccgaggc
     7141 gctgaggaga tccacatgga gctccccgac ggtaaaaggc aggtgcccca gggtttgaac
     7201 ggcgtcggtg tgaaaataca ccccccgctc ccgggcgatc ctgccgattt cctctatggg
     7261 ttgaatcgtt ccgatctcgt tgttggcatg catgaccgag ataaggattg tttttccggt
     7321 aatcgtcttc cggacgtcgt cgggatcgac caggccatct ccatccacgg gcaggatcgt
     7381 aatctcgaaa ccctccttct ccaggaaatg gcagggctcc aggacggcgt gatgctcgat
     7441 gggggaggta atgatatgat tccccttgcc ctgccgggcg taggccaccc ctttgacggc
     7501 cgtgttgttg ctctccgtcc cgccgctggt gaagacgatc tctccgggcg ccgcccccag
     7561 aaaggaggca atccgttccc gagcctcctc caccgcccct ttgttctcct ggccgaaggc
     7621 gtgcatactc gccggattcc cgaaccggtc ggaaaagaac ggcaacatgg ccgccaccac
     7681 ctcggaggcg cagggggtgg tcgccgcata atccagatat atcctttcca tcttcactta
     7741 ccttcctttc ttttcgctag gacaaagacg gcgttcaggg caaagagatt gggccagaag
     7801 gtgacggggc cgtttgctcc gataaagtgg gaagtcaaaa catggagccc cttgtccagg
     7861 cagaagatcc ggaaatccct gatgctgagg aaccggacat tcggcgtgtc gtaccacagg
     7921 taaggcagcg aggctgtaac cggggagcgc ccccccaggc cgagcatgat ccgggcgcgg
     7981 aagtacgcga aatttgggaa acccacgatt acccgcttgc ccacccgcag ggactcggca
     8041 atgacgaaat ccaccttacg cacctcctgg agacactgat tgaggatcac gtcgtcaaag
     8101 gcgccgtcgg gatactccgt caaacccgtt tcaatgtccc cctgaaagac gctgagccct
     8161 tttttcacgc attcgtaaac cgaccgaccg tccatctcta tgccctggac ccgggcatgt
     8221 ttgcctcgga ccagatggga gagcaactcc ccggtgccgc aaccgagatc gaggacccgg
     8281 gattccgggg ggatggcctc gtatatcacc cggtggtcgg cacgttccgg cgtatcgttc
     8341 cgctccatat tcatgcaaat cccccgacag tcgtgccgcg tcccggccaa ctatgataac
     8401 cctgctgggt cacccgtaag aagttgcgga tcagttccgt ctgctccgcc gtctccacga
     8461 ggaaggcgtc gtggccgtag gtggatttca gctcacagta agcgacgtca acctgcctgc
     8521 ccttcaactc ccggacgatc tcctgggact gataggaggg ataaagccaa tccgacgaaa
     8581 aggagatgac gaggaaacgg gtggcgatca gtttgtcttg gcggatcagc cttcccgcgg
     8641 acagatcgag gtagtccatc gccttggtga tgtagagata ggaattggca tcgaagcgct
     8701 tgacgaagtt cgccccccgg taacgcagat agccctccac ctcgaaatcg gcgtcgaaat
     8761 tgaaactgaa gacgccgttc ttcaaccggc gggagaattt ttcctccatg gagcgatcgc
     8821 tcatgaaggt gatatgtccg atcatccgcg ccacggcaag tcccttttcc ggttgcccgt
     8881 gatcatagta atcgcccttc cgccaggcgg gatcagccat gatggactga cgcaccacct
     8941 cgttgaaggc gatctgctgg ggtgaatgct tcagggccgt ggcgatgggg atggccgccc
     9001 gcacccgttc cgggtaggag gccgcccact ggaggacctg cattcccccc atggagccgc
     9061 cggcgacgca gagcagcctg tcgatgccca gatgatcgat gagccggcgc tgggtgttta
     9121 ccatgtcggc gatggtaatg aacgggaatc ccagggcgta gggacggccc gtggttggat
     9181 tgatcgcagc cggcccggtg gaacccttgc agccgccgat gatattggag cagatgacga
     9241 agtatttgtt cgtgtcgaac cctttgcccg ggccgatcat ctcctcccac cagcccgtgg
     9301 cgccgtcctc cccatgcacc ccggcggcat gggcgtcccc cgacagggca tggaggatga
     9361 ggatcgcatt tgaccgccgg gcatcgaggc gtccataggt ctcataggcc agggtgatgg
     9421 gaccgagcct ttccccgcat tccaggacta gctcgttcgg gggatgggcg aaggtataat
     9481 acttcgtttc caccgtgcca atgtcgtttt gaaaattatt cttctttaaa gccgtcgata
     9541 ccattgccaa tccagttaat aaaagaaaac ccactaacac gcccttgctt ggtcttagtg
     9601 ggtctcggtt ccattcgtgg gcaactatca actcattgcc ggccctcttt actccccaga
     9661 ccaaccgtcc tgcggggaca acggcgcatt atcatatgga aaacgctcat tttcgatctc
     9721 tttgcccggt tatatcctta cccatggcta aataaacatg cccgtatcgg gaaccttggt
     9781 gcgctgcctt tgcaccagat tttccagggt gatacccatg aggatctcac cgaccttctg
     9841 ggatgcctct ccccaaacct cccgggatat gcagacctcc gcccggtcgc agcaggaggg
     9901 gttctccacg caatccacga agcagatcgg accttcgagg gttctcacca catccatgag
     9961 attgatgctt tgcggcggcc gggagaggta gtagccgccc ttcgcccccc ggaccgtggt
    10021 aatcaggccc gcggatttca gtggcggcac gagatgccag agatatttct ccgagatccc
    10081 ctggcgctgg gcgatatcct tcaagaagat cggcccttcg ccataatggg aggccagatc
    10141 gaacatgaac cgaacgccgt atctgccccg ggtagaaagc ctcatttttc tacattctcc
    10201 atagttttag tggagattaa ggtattagca ataatttgtc aaggaatttt tttcttgaca
    10261 aagtgatcga catctaatat ctaccaaacc gatagaattt ctggtatata ataattatga
    10321 aacatttatc aatacttgcg ttgtcaaaat ggggacagga cctccaacct gccggaaaca
    10381 tccccgccat gccgatggtc atgtgttgtt ccatgcgtaa tgccggccgg gggcggatag
    10441 gaggtccggg catgtaattg cctgactgga taacctcacg caatcaaaaa cccgcctccg
    10501 ggagacatca gtcgcaggat ggcgggtttt tttattcaat caaatacagg aggtaattaa
    10561 atgacgctaa gaagattgga aacactggca ttgcactccg gacaggagtc gcccgatccg
    10621 gccacggggg cccgggcggt tcccatttat caaaccactt catacgtgtt tcagagcacg
    10681 gagcatgcgg cgagcctctt tgcccttcag cagttcggga atatctatac ccggatgacg
    10741 aaccctacta cggaggtctt cgagcagagg atcgccgccc tcgagggagg gaccggcgcc
    10801 ctggccacgg cctcgggaca ggcggccata accctcgcca tgctgaacgt cacccggttg
    10861 ggagacgaga tcgtcgccgc cgacaatctc tatgggggga catatcagct ttttcactat
    10921 accctcccga agctgggccg gaccgtgaag tttgtcgatt ctcggcagcc cgagcaattc
    10981 cggcgggcga tcacaaataa gacccgagcc gtttatgccg agacgatcgg gaatcccaaa
    11041 ctcgacgtcc ccgatttcga ggccatcgcc aagatcgccc atgcggcggg catcccgttt
    11101 attgttgaca ataccgtcgg tgtcggcctg gtccggccca tcgactatgg ggccgacatc
    11161 ctggcgacct cggcgacgaa attcatcggc ggccacggca cctccatcgg cggcgtcatc
    11221 gtcgacgggg ggaagttcga ttgggggaac gggaaattcc cggaattcac cgcgccggat
    11281 ccgagctatc acggcctgag attctgggat gccttccgcg cagttccagg tctgggcaac
    11341 gtgaccttca tcatcaaggc ccgggtccag ctcctgaggg acatcggtgc cagcctgagt
    11401 cccttcaacg ccttcctgtt cctccagggg ttggagacgc tctatgtccg ccagcggcgg
    11461 cacggggaaa acgccctggc cgtggcccgc ttcctcaaag accaccgggc ggtaaactgg
    11521 gtcaactatc ccggcctgga agatcacccc agccatgctc tcgccgtcaa atacctgaaa
    11581 ggcaactatg gcgccctcgt cggcttcggc atcaagggcg gcctggaggc gggtaagaaa
    11641 ttcatcaatt ccgtaagact cctttcccac ctggccaata tcggcgacgc caaaagtctg
    11701 gtgattcatc cggcctcaac cacccaccag caactgacgc gaacggaacg ggaagccacg
    11761 ggggtaacgg aggattttat ccgtctttcc atcggcatcg aggatgtgga ggacatcaag
    11821 gaagacatcg accaggcttt gcggcaggcc gcggcctgag gaaagggagc cgaaaggagc
    11881 gcaccaatga agatatttgc cgacattacc aagaccgtgg ggggaacgcc cctggtggag
    11941 ctgcggcgcc tgtccgccgg ggccagggcg cggatcgtcg tcaaggtgga atccttcaac
    12001 cccctgtcca gcgtaaagga caggatcggc gtcgccatga tcgacgccgc ggaaaaaagc
    12061 ggtctgctcc agtcgggatc ggtcctgatc gagccgacga gcggcaatac ggggatcgcc
    12121 ttggcctttg tggcctccgc ccggggttac cgtctgatcc ttaccatgcc ggacaccatg
    12181 agtctggaac ggcggcagct cttggagatc ttcggcgcgg aactggtcct gacgccgggc
    12241 ggggaaggga tgagcggggc ggtgtgcaag gcggaggagt tggcgggggc gattcccggc
    12301 gcggtcatgc ttcagcagtt ccagaacccg gccaatccag agatccatcg gaaaacgacg
    12361 gcggaagaga tctggaacga tacggacggc ggggtggata tcctcgtcgc cggcgtaggc
    12421 acggggggga cgattacggg gattgccgag gtcatcaaag agcgcaagaa gggtttccag
    12481 gccattgccg tggagccgga aagctccccc gtcctttccg gcggcaagcc gggaccccat
    12541 aagatccagg gtatcggcgc cggattcatc ccccgggtac tcaatcgggg gatcatcgat
    12601 gagattgtaa aggtccgcaa tgaaaatgcc ggcgccatcg cccggcgcct ggccagggag
    12661 gaggggatgc tggtaggcat ttccggcggc gccgccttgt gggccgccct ggaggtggcg
    12721 aaacggcagg agaatcaggg taaactcatg gtggtcattc ttcccgatac gggggaacgg
    12781 tatctttcca cctggttgtt caaggagtga gattccggga cgacggatga aaaatcggaa
    12841 acaggaaccc ggcgtggtgg cgaggagtgc taccgggaat ca
//
LOCUS       DAOSMF010000046        12742 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_5727_length_12742_cov_24.648538, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000046 DAOSMF010000000
VERSION     DAOSMF010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 12742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12742
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5727_length_12742_cov_24.648538"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2187
                     /locus_tag="PLT63_06175"
     CDS             <1..2187
                     /locus_tag="PLT63_06175"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOD98345.1"
                     /translation="EAEAKARILLDRNLATHTYFSHVLKPRLFEWTAPFRSPEYFDPS
                     WMSSTYAIRGIHKYFLELNSAGYYLKDAAVDARSPENEADDYERAFLAALKNDRTLDS
                     RSEVRTIDGKPYLAVMRRGESMEESCLRCHSTPGNAPADLLKHYGSERSFNRKAGDLV
                     SAISLRIPLAATYEGMNKTFVRITLLLTAILGILFVVHSFIYRRFLVLPIAMLRDKAK
                     AIAASSHHLGEEIPEPAGGELRELAGAFNEMSSQLRISKDSLEDRVRERTGELNTAKE
                     RLEREVAQRRTAEDKLKESNKELTCLYSLAEIVEKTDAVEDVLRKSVDLIPGGLHYSE
                     SACARIIWRKREFRTGNFQETNWRMAAHIIRQGESVGTLEVCYRNDVSGDGNGPFLEE
                     ERKLIDAIAEYLGRVIDHKEANEKLKASEAQVRLLLNSTAEAIYGIDLQGNCTFANQA
                     CLRILGYQDIGQLLGKNMHDLIHHSYPNGTPMAVKDCRIYQAFREGKGVQVDDEVLWK
                     ADGSSFPVEYFSYPQIVAGGVSGAVVTFLDITQRKRAEEKLRESERRYRELSIIDSLS
                     QLYNSRYFYTQMQMETDRVNRYGEPLSLILLDIDNFKAFNDTYGHVEGDRVISGLGQL
                     IKRYLRKTDSAYRYGGEEFTVLLPSTKGSDGIITAERIRREFKKETFAPAPGKSIRMT
                     VSIGIAQYRPHEDPKAFVKRVDRLMYRAKRQGKDRVCHEANTEKNP"
     gene            2219..2806
                     /gene="lexA"
                     /locus_tag="PLT63_06180"
     CDS             2219..2806
                     /gene="lexA"
                     /locus_tag="PLT63_06180"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HOD98346.1"
                     /translation="MKAKRTVPSVAEAIDNFRRRHRRMPSYAEMLPLLGVRSKSVVHF
                     WINKLLENGLLEKDDKGYLTPGSRARGIPMVGEVAAGFPSPAEEELRDIISFDEYLVT
                     KPESSFLLQVSGDSMIGEGIREGDLVIVEKGREPRNGDVVVAQVDGEWTMKYFRKQGK
                     QVRLEAANPNYPPIIPKAELLMGGVVTAVIRKYHL"
     gene            2864..3091
                     /locus_tag="PLT63_06185"
     CDS             2864..3091
                     /locus_tag="PLT63_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98347.1"
                     /translation="MKLRAYADDIYGFPAAARNDRRLRAGLSPARWLRRLRDYDWERI
                     IPGLERSLDAFCTGVILLAVLYFLPILFLRY"
     gene            3271..4581
                     /locus_tag="PLT63_06190"
     CDS             3271..4581
                     /locus_tag="PLT63_06190"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOD98348.1"
                     /translation="MSSLPAELIILLILVLINGAFSMAEIALVSSRKAKLRVEAENGD
                     AGAEKALNLAEEPGSFLSTIQIGITLVGILSGAYGGATVAKSLSAYLQQYPALRPYSD
                     TLGLTVVVLAITYLSLVLGELVPKRLALFKAERIATLAARPVYYLSLAAYPLVWILNV
                     STEAFLKVMNIKKSESSVSEEEVKVLLEEGAEAGIFHAMEHEICHRAFHIDDLGVRDL
                     MTPRPDILWLDAMKPFAYNLEIIRTGEHTCFPVGRGSLDQIIGFLHLKDLLKHEIDPG
                     KGNVDLLPLLRKTLYFPETISAWQVLEQFKTSPIHMAMVVDEYGIIQGLVTISDVIET
                     IVGDIPSLSEGEESGIVRREDGSWLVDGAVSIDDFKAHFKIQKLPAEVRADFHTLGGF
                     IMSYLERIPTTGERFQWDDWRFEIVDMDGHRIDKVLVSGPKETS"
     assembly_gap    4734..4833
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4834..5802
                     /locus_tag="PLT63_06195"
     CDS             <4834..5802
                     /locus_tag="PLT63_06195"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HOD98349.1"
                     /translation="VIASTGSYLPETVIPNEGLTQFSREAQALIGSKTGVQARRHAGE
                     HQTTSDLALEAARSCLEKISFPAADVAGIILSTSSPDRIQPATATRLQHLLGARGSFA
                     FDINSVCAGSTYGLHLADALIRTGQYENILFVAAETYSRILNHKDFSTYPYFGDGAGA
                     VLLQGREDSARGVLNSVLGTDGSRSETICVPGGGAMMPYEKITNPRAAYFRMKGEEVF
                     NFTLDKGPLVIRQAAAAAGVALEEIKCFLCHQANIHILWGIADILDVPRERFYVNLDR
                     YGNTASASVLIALDEARTEGVVGPGDLVITAAFGGGLSWGANLIRI"
     gene            5834..6100
                     /locus_tag="PLT63_06200"
     CDS             5834..6100
                     /locus_tag="PLT63_06200"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HOD98350.1"
                     /translation="MTDTLKKLKTGIEDLFPETEGMVIDAETRLGDIPDFDSMAAVNL
                     QNFIVETFNVTIPLDLFGEDTTLGEIAARIENPKKLAAGGEKKA"
     gene            6103..7749
                     /locus_tag="PLT63_06205"
     CDS             6103..7749
                     /locus_tag="PLT63_06205"
                     /inference="COORDINATES: protein motif:HMM:NF018827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HOD98351.1"
                     /translation="MDPLDILTSLWNFQRSWLDRSEETNERVIDLFRRLEEATREEFP
                     HLLSSELPAEDKKNAGEVFLEYVRKNAKLNRRFHAILDIWLRELVELTRDLNDRDRQR
                     ARFWIQQMSSAMAPPNFFWTNPAAMKKFLETGGASLMQGLGRAWEDLSRGDYLSRITD
                     ETAFRVGENIAITPGAVVFRNELMELIQYEAATETTYTLPVVLIPPWINKYYIFDLTP
                     QTSLVRYLRDQGFTVFTISWKNPTEAMRKVAFSDYMFAGALAAVATARHICRSPQVHA
                     VGYCIGGTALAALMAWLNRGRRRNSPGPVADWTLFSTLVDFSEPGDLGVFISDRSLAA
                     TEALMRMDGFLDARYLNWIFRILGADSLIWRNYIQNYLYGAAPPKSDMLFWNSDSTRL
                     PEAMCSYYLREFYLHNNLAKGDALTLGERPIDMGRVVQPLYAVGTQLDHICPWRGAFR
                     TCGIVAGQRRFVLSSEGHITGIINPHSEGSRRKYWTGEADLPEEPEAWLRRQEERRGS
                     WWPDWTAWLSQRDNSRRPPPPLGNRKYPPRDRAPGIYVHE"
     gene            7796..8740
                     /gene="fabD"
                     /locus_tag="PLT63_06210"
     CDS             7796..8740
                     /gene="fabD"
                     /locus_tag="PLT63_06210"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOD98352.1"
                     /translation="MEKWALIFPGQGSQFVGMGADFHRDCPAYREVLSRVREITGVDI
                     ARLCLEGPQEDLDQTVNTQLAVLAFEIGAYTLFREQAGEIDPIAAAGHSLGEFSALYA
                     AGILTLEDAVTIVQARAKRHQEALPAGRGAMAAILGLTGVETAAVCRECVLPGETLDL
                     ANLNAPNQIVISGDVRAVARVGGAAAQKGIKKFVPLPISVPCHSRLLGAAAELFRADL
                     EQIAFHPPRLPVIPNYDPRREHSPAASRELLKMQMISPVRWQETVEMLISKGVTTFVE
                     IGPRRVLSGLVKNIDRRPRLLYAGDGESLRKTVAALLT"
     gene            8913..10292
                     /locus_tag="PLT63_06215"
     CDS             8913..10292
                     /locus_tag="PLT63_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOD98353.1"
                     /translation="MKKRFAVYFGVAILGIAALAVIAGPYLNSQVSAALDRSIYKNLK
                     NFTEVLDIVEKNYVEPVNAKNLLQGAINGMIKNLDPHSSFMTPEMYKELELETRGSFG
                     GIGIEITIHKDVLTVVSPIEDTPAFQAGVKSGDKIMMIDGKSTKDINIMDAVKRLRGE
                     KGTKVTISVLRGNENKLREFVITRAIIKVISVKHKLLDGGIGYIRVSSFQDKTADDLK
                     KALREMTAKKEPLKGIVLDLRNNPGGLLNQSIEVSDIFLKSGGIVSTKGRSKGMESKV
                     SARDKGDEFTAPMIVMVNEGTASAAEIVSGALQDNGRALILGTQTFGKGSVQTVIPLE
                     DGSALKLTTARYYTPKGRSIQAEGIAPDVTVKYIRPGSNGEDEDHEPIREKDLKGHIK
                     SAQEQAKPGEEADREGELLQKRLKNSELRKDLPLKRPFGDVNADNQLKAAVDMLKSWD
                     IFKKNLVKN"
     gene            10304..11977
                     /locus_tag="PLT63_06220"
     CDS             10304..11977
                     /locus_tag="PLT63_06220"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD98354.1"
                     /translation="MNTIAKAAVYLAFLSMILGAAPDLYGKSRPDYLAAYHYSLGVLH
                     MLDENPQEAIKEFEKARRYDPAAPEIAIDLAAAYVEKGEAAKAIHLCEQVRKDHPDHV
                     DLNLILAGLYMNNKNYPRAVEIYKQVVHQEPRNVSAPFYLGVIYGDAKQYPEAIAMFE
                     QVLRLDPQHLMAAYYEARVFMEMKNYQGAEKMLKKTLEIRPNFEAALIDLALAYEKQR
                     HTSLAIDTFKDYLFLHPDKLHVRIKLGEMYLREKRYAEAEKEFQRILRSDPDNQEVLL
                     TLGLLFIESGRYDQAAATFEKLVRRYPADQRYAYLQATAYEEKRLYAPALAIMEKIPP
                     TSDLYSSARIRIAMIRKKEGKISDAIIVLEQAIKVKRDVPGFYVVLSSLYEDQQDRVG
                     AERILKEGMVILPENTDLRYSLGVLYEKTGHFEASIQQMRTILKIDPDHADALNFIGY
                     SYADRGMHLGEAEKLILKALQLKPGNGYIIDSLGWTYFKQNRIDLALKYLQEAAAILP
                     GDGAIAEHLGDAYLKAGRRQEALTAYRRALKINPASTTLPQKIDTLTDK"
     gene            11974..>12742
                     /locus_tag="PLT63_06225"
     CDS             11974..>12742
                     /locus_tag="PLT63_06225"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HOD98355.1"
                     /translation="MSRIRILVLFLFFSLIAGGCAHETGTSKPGEAINLSPEEALARL
                     DLRSQDRRLLKAIAEIAVITETGRYPLKLAILAWRPDSLRLEAIPVIGLPNFFLAAHA
                     GRLAVYLPGTGEYYTGAAVPENLARFFPLSLSISDLISLLTGGRPALPMEEQPRVEGF
                     HEGASYRLDISSRDRRFRQSLWIDGKTGYLRRCKQTAGNCGDDALNVYFDAYVPVDGV
                     PMPQRIRVKMKGEEERTVVLEYESVTLETMTAAGAELF"
BASE COUNT         2830 a         3779 c         3643 g         2390 t
ORIGIN      
        1 gaagcggagg caaaggcccg cattctcctc gaccgcaacc tggccaccca cacctatttt
       61 tcccatgttt tgaagccccg actttttgaa tggaccgcac cttttcgctc ccccgaatat
      121 ttcgatcctt cctggatgtc ctccacctat gcgatccggg gtatccataa atacttcctg
      181 gaactcaact cggccggata ttatctcaag gacgccgcgg tggatgcccg gagtcccgaa
      241 aacgaggccg acgattacga gcgggccttt ttggcggcct tgaaaaacga ccggacgctg
      301 gattcacgtt cggaagtgcg gaccatagac ggcaagccgt atttagcggt catgaggcgg
      361 ggcgaatcga tggaagagtc ctgcctgcgc tgtcacagca cccccggcaa cgcccccgcc
      421 gatcttctca agcattatgg ttccgagcgg agcttcaacc ggaaggcagg cgacttggtg
      481 agcgccatat ccctgcgcat acctctcgcc gccacctacg agggcatgaa caaaaccttc
      541 gtgcggatta cgctgctgct cacggcaatc ctcggtatcc tctttgtcgt tcacagcttt
      601 atctatcgcc gtttcctggt tctaccaatc gccatgctcc gcgacaaggc aaaagcaatc
      661 gcggccagtt cccaccatct cggcgaggag atcccggagc ccgccggcgg ggaattgcgg
      721 gagcttgccg gggcgttcaa tgagatgtcc tcgcagttgc ggataagcaa agatagtttg
      781 gaggaccggg tccgggaacg caccggggaa ctgaacactg cgaaggagcg cctggagagg
      841 gaagtggcgc agcgacggac agcggaggat aagctgaagg aaagcaacaa ggaattgaca
      901 tgtctctatt cccttgccga gatcgtggaa aaaaccgatg ccgttgagga cgtattgcgg
      961 aaaagcgtcg atttgatccc cggcggctta cattattccg aatctgcctg cgcacgcatc
     1021 atttggcgga agcgggaatt ccggacgggg aattttcagg aaacgaactg gagaatggcg
     1081 gcccacatca tccggcaagg tgagtccgtg ggcaccttgg aggtctgcta tcgaaacgat
     1141 gtgtccggcg acgggaatgg accgttcctc gaggaggagc ggaaactgat cgatgcgatc
     1201 gcagagtatc tggggagggt catcgaccat aaggaggcga atgaaaagct aaaggcatcg
     1261 gaagcgcagg tgcgcctgct cctgaattcc acggccgagg cgatctacgg catcgatctt
     1321 cagggaaact gcacctttgc caatcaggcc tgcctgcgga tcctgggata tcaggacata
     1381 ggccagttgc tgggaaaaaa catgcacgac ctcattcatc attcctatcc gaacggaacg
     1441 ccgatggcgg tgaaagattg ccgcatctac caggcattca gagagggcaa aggggtgcag
     1501 gtcgatgatg aggtattgtg gaaggccgac ggcagcagtt tccccgtgga atatttttcc
     1561 tatccccaga tcgtcgccgg cggggtgtcc ggggccgtgg tcaccttcct cgatataaca
     1621 cagagaaaac gggcggaaga aaaactccgg gaaagtgaaa gaagatatcg tgaactaagc
     1681 atcatcgaca gcctctccca gctctacaat tcccgctatt tttacaccca aatgcaaatg
     1741 gagaccgatc gcgtcaaccg ctacggagaa ccgctgagcc tcatcctgct cgatatcgac
     1801 aatttcaagg ccttcaacga cacttacggt catgtcgagg gggaccgggt aatcagcggg
     1861 ctcggccagt tgatcaagag ataccttcgc aaaacggatt ccgcgtaccg ttacggcggc
     1921 gaggaattta ccgtcctgct gccatcaacc aaaggcagcg acggcatcat taccgcggaa
     1981 agaatccgca gggaattcaa aaaagaaacc tttgccccgg cacccggcaa aagcatccgg
     2041 atgacggtaa gcatcggcat cgcccaatac cgaccccacg aagacccgaa ggcctttgtt
     2101 aagcgcgttg accgcctcat gtaccgggcc aagaggcagg gcaaagaccg ggtctgtcat
     2161 gaggcaaata ccgaaaaaaa cccttgacaa tagaacgaat gttctatatg gtggggccat
     2221 gaaagcgaag agaaccgtcc ccagcgtggc cgaggccatc gataacttcc gccgccggca
     2281 ccggcggatg cccagttacg cggagatgct tccgctcctg ggggtccgct ccaaaagcgt
     2341 tgtccacttc tggatcaaca agctgctgga aaacggcctg ctggaaaagg acgacaaggg
     2401 ctacctgacg ccggggagcc gggcccgggg gattcccatg gtgggggaag tggccgccgg
     2461 ctttccctcc cccgccgaag aggaactccg ggacatcatc tccttcgatg aatacctggt
     2521 gaccaagccg gaatcctcct tcctcctcca ggtcagcggc gactccatga tcggcgaggg
     2581 cattcgcgag ggagacctcg ttatcgtcga gaagggacgg gagccgagaa acggagacgt
     2641 cgtcgttgcc caagtggacg gcgaatggac gatgaaatat ttccgcaaac aagggaaaca
     2701 ggtccgcctc gaggcagcca atcccaacta tccccccatc atcccgaaag cggaacttct
     2761 catggggggt gtggtaacgg cggtgatcag aaagtatcac ctctaaaatt gacgaacatt
     2821 aatcacctcg aaggaacaat ttgagaaagg cagaatatca gtcatgaaac tacgcgctta
     2881 cgcagatgac atatacggct tccccgccgc cgcgcggaac gatcgtcgtc tccgggccgg
     2941 tctttccccg gcgagatggc tccggcggct ccgggactat gactgggagc ggataattcc
     3001 ggggctggaa aggtctctcg acgccttttg caccggggtc attctcctgg cggtcctcta
     3061 ttttctcccc attctgttcc ttcgttattg aggtccctct cgccatccca agaaccgcct
     3121 cccgaaaaag cggcgacaaa gcggaaaggc caccattcct gatagtacat tttttcgatg
     3181 cggagtccgg cgggagtctt tccttgactc cataacaaaa gccgcctata ttgatcgcaa
     3241 atatcgtttg aattccggaa atcgcaagtc atgtcttctc tccctgcaga acttattatt
     3301 ctgctgattc tggtcctgat caacggggct ttctccatgg cggagatcgc cctggtctct
     3361 tcccgcaagg ccaagctccg ggttgaagcg gaaaacggcg atgccggggc ggagaaggcc
     3421 ctgaacctag ccgaagaacc ggggagtttt ctctccacca ttcagatcgg catcaccctc
     3481 gtcggcatcc tctccggggc ctacggcggc gcaaccgtgg cgaaatccct gagcgcctac
     3541 ctgcagcagt atccggcgct ccgcccgtac agcgacaccc tcggcctcac cgtcgtggtg
     3601 ctggcaatca cctatctctc cctggtcctg ggggagttgg ttcccaagcg gctggccctg
     3661 ttcaaggcgg agcggatcgc caccctggcc gcccgtcccg tctattacct ttccctcgcc
     3721 gcctaccccc tggtctggat tctcaatgtc tccacggagg ccttcctgaa ggtcatgaac
     3781 ataaagaaat cggagagctc cgtttcggag gaagaggtga aggtgctcct tgaagaggga
     3841 gctgaagcag gcatctttca tgccatggag cacgagatct gtcaccgggc cttccacatc
     3901 gacgatctgg gcgtgcggga tctcatgacg ccccgtccgg acatcctctg gctggatgcc
     3961 atgaagccct tcgcctacaa tctggagatc atccggactg gcgaacacac ctgctttccc
     4021 gtaggccggg gcagcctcga ccagatcatc ggctttctcc atctcaaaga cctcctgaag
     4081 cacgagatcg atccgggaaa ggggaatgtc gatctcctgc ccctgttgcg gaagaccctc
     4141 tattttcccg agacgatctc cgcctggcag gttctggaac agttcaagac ctctcctatc
     4201 cacatggcca tggtggtgga tgaatatggc atcatccagg ggctggtgac catcagcgat
     4261 gtaatcgaaa cgatcgtggg cgacatcccc tccctgagcg agggagagga aagcggcatc
     4321 gtacgccgcg aggacggttc ctggcttgtg gatggcgccg tctccatcga cgacttcaag
     4381 gcccatttca agatccagaa actgccggcg gaggtcagag ccgatttcca caccctgggc
     4441 ggcttcatca tgtcctatct ggaacggata cccaccacgg gtgagcgctt ccagtgggac
     4501 gattggcggt tcgagatcgt ggatatggac gggcaccgga tcgacaaggt gctggtcagc
     4561 ggccctaaag agacctcatg aagcgttttt tgacaggata gttccgctgt gatagaaaac
     4621 ctttcctgtc gatggtccat ttgatcaaga agcgagggaa agcgctatga taagaggggt
     4681 cattgcctcc accggttcct acctgccgga gaccgtcatt cccaacgagg ggcnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngtcattg cctccaccgg ttcctacctg
     4861 ccggagaccg tcattcccaa cgaggggctc acccagtttt cccgggaggc ccaggccctg
     4921 atcggcagca aaaccggggt ccaggcgcgg cggcacgccg gagaacatca gaccacctcc
     4981 gacctcgccc tcgaggcggc ccgatcctgt ctggagaaga tctccttccc ggccgccgac
     5041 gtcgcgggga tcatcctttc cacctcttcc cccgaccgca tccagccggc cacggccact
     5101 cgtctccagc accttctcgg ggcccgggga tccttcgcct tcgatatcaa ctccgtgtgc
     5161 gcgggcagca cctacggcct ccacctcgcc gacgccctga ttcggacggg gcagtacgaa
     5221 aacatcctct ttgtggccgc cgagacctat tcccggatcc tcaaccacaa ggatttctcc
     5281 acctacccct atttcgggga cggggcggga gcggtgctgc tgcagggccg ggaagactcc
     5341 gcccgcgggg tgctcaactc cgtcctgggc accgacggca gccgcagtga aacgatctgc
     5401 gtccctggcg gcggcgccat gatgccctac gaaaagatca ccaacccccg ggcggcgtat
     5461 ttccgcatga agggggagga ggtgttcaac ttcaccctcg acaaaggccc cctggttatc
     5521 cgccaggccg ccgctgcggc cggcgtggcg ctggaagaga taaaatgttt cctatgccat
     5581 caggcgaaca ttcacatcct ctggggcatc gccgacatcc ttgacgtccc tcgggaaagg
     5641 ttctatgtaa atctggatcg ttacgggaac accgccagcg cctcggttct tatcgccctg
     5701 gacgaggcgc ggacggaggg ggttgtcggc ccgggggatc tggtgattac ggcggccttt
     5761 ggcggcggct tgtcttgggg agccaatctg atccggatct gaggaaggaa aacatccaaa
     5821 aggaggtaaa ggcatgaccg atacattaaa gaaactcaag acgggcatag aagacctctt
     5881 cccggaaacg gaaggcatgg tcatcgatgc ggaaacccgt ctgggggaca tccccgattt
     5941 cgattccatg gcggcggtga acctgcagaa ttttatcgtg gaaaccttca atgtcaccat
     6001 ccccctcgac ctcttcggcg aggacaccac ccttggggag atcgccgccc gcatcgaaaa
     6061 cccgaaaaag cttgccgccg ggggagagaa gaaggcctga cgatggaccc cctggatatt
     6121 ttgacttccc tctggaattt ccagcgttcc tggctggacc gctcagaaga gacaaatgaa
     6181 cgggttatag atctattccg gcgcctggag gaggcgaccc gggaagagtt tccccatctc
     6241 ctgtcctcgg agctgccggc ggaggacaag aaaaacgccg gcgaggtctt cctcgaatac
     6301 gtccggaaga acgcaaaatt aaaccgtcgc tttcatgcca ttctggacat ctggctccga
     6361 gagttggtgg agttgacccg ggatttgaac gaccgggatc gtcaacgggc ccgtttctgg
     6421 atccaacaga tgtcgagcgc catggcaccg ccgaatttct tctggaccaa ccccgcggcc
     6481 atgaagaaat tcctcgaaac cgggggcgcc agtctcatgc aggggctggg ccgggcctgg
     6541 gaagacctga gcagggggga ttatctctcc cggatcaccg acgagacggc ctttcgggtg
     6601 ggggaaaata tcgccattac ccccggtgcc gtggtttttc gcaacgagct catggaactc
     6661 atccagtacg aagccgccac ggaaacgact tataccctcc cggtggttct gatccctccc
     6721 tggatcaaca agtattacat cttcgatctg actccacaga ccagcctggt ccgttatctc
     6781 cgggatcagg ggtttaccgt cttcactatc agttggaaga atccgacgga ggccatgcga
     6841 aaggttgcct tctccgatta catgtttgcc ggggcgctgg cggcggtggc aacggcacgg
     6901 cacatctgcc ggagtcccca ggtccacgcc gttgggtact gcatcggcgg cacggccctg
     6961 gcggccctca tggcctggct gaaccggggt cgccggagga acagccccgg gccggtggcg
     7021 gactggacgc tgttttcgac cctggtggat ttcagcgaac cgggggatct gggggttttc
     7081 atcagtgacc gctcccttgc cgccaccgaa gccctcatga ggatggacgg tttcctcgat
     7141 gcccggtacc ttaactggat attccggatc ctcggcgccg acagcctgat ctggagaaac
     7201 tatattcaga actaccttta cggggccgcc cccccgaagt ccgacatgct cttctggaac
     7261 agcgacagca cccgcctgcc cgaggccatg tgctcctact atctccggga gttctacctc
     7321 cacaacaatc tcgccaaagg cgacgccttg accctggggg aacgtcccat cgatatggga
     7381 agggtcgtac agcccctcta cgccgtggga acccaactgg accatatctg cccctggcgc
     7441 ggcgcctttc gcacctgcgg gatcgttgcg ggccaacgcc gctttgtcct ctccagcgag
     7501 gggcacatca ccgggatcat caacccccac tccgagggat cgcggcgtaa atactggacc
     7561 ggggaggcgg atctcccgga ggaacccgag gcctggctcc gccggcagga ggaaaggcgc
     7621 ggctcctggt ggccggactg gaccgcctgg ctgagtcaac gcgacaactc ccggcggccg
     7681 cccccgcctc tgggaaaccg gaaatacccg ccccgggatc gggcgccggg catctatgtt
     7741 cacgaatgaa tgccgtgaag agatccggta ccgacaaccg gaggagagat cgataatgga
     7801 aaaatgggcc ctcatctttc cgggacaggg atcccagttc gtaggcatgg gcgcggattt
     7861 ccaccgggac tgccccgcct accgggaggt cctgtcccgg gttcgggaga tcacgggagt
     7921 ggacatcgcc cggctctgcc tggagggccc ccaggaggac ctggatcaga cggttaacac
     7981 ccaactggcg gtactggcct tcgaaatagg ggcctatacc ctcttccggg agcaggcggg
     8041 agagatcgat ccgatcgcgg cggccggaca cagcctgggc gagttcagcg ccctctatgc
     8101 cgctgggatc ctgaccctgg aagatgcggt aaccattgtc caggcccggg cgaaacgaca
     8161 ccaggaggcc cttcctgccg gacgcggggc catggccgcc attctgggcc tcaccggcgt
     8221 ggaaacggcg gcggtctgcc gcgaatgtgt cctgcccggt gaaaccctgg atctggccaa
     8281 cctcaacgcc ccgaaccaga tcgtcatctc gggagatgtc cgggccgtcg cccgggtggg
     8341 gggagcggcc gcccaaaagg ggatcaagaa gttcgtcccc ctccccatca gcgtcccttg
     8401 tcattccagg ctcctcggcg cggcggccga acttttccgc gccgatttgg aacagatcgc
     8461 ctttcatccc ccccgcctgc cggtcattcc caattacgat ccccgacggg aacattctcc
     8521 ggcggcatcc cgagagttgc tgaagatgca gatgatctca ccggtgcgct ggcaggaaac
     8581 ggtggagatg ttgatctcca agggcgtaac gaccttcgtg gaaatcggcc cccgccgggt
     8641 cctttccgga ctggtcaaga atatcgaccg gcggccccgc ctcctctacg ccggcgacgg
     8701 tgaatccctt cggaaaaccg tcgccgctct tttgacatag caaacattca tcccaataat
     8761 tatggcattt ttcccgcaaa gcatggtatg aggaggagta aatcatttta cccttaaggg
     8821 gatgcgccgg ccttccgcac cgtacctgat aaacatgtcc ggggacgaca atgatggacg
     8881 aacatcccac tcccgggtgg aggaagagac gaatgaagaa gagatttgcc gtctatttcg
     8941 gggtggcgat cctgggaatc gccgccctag cggtaattgc cggcccatat ctgaacagcc
     9001 aggtttccgc ggccctggac cgttccatct acaaaaatct aaagaatttc acggaagttc
     9061 ttgatattgt agaaaagaat tacgtggagc cggtgaatgc caagaacctg ctgcagggag
     9121 caataaacgg gatgatcaag aatctcgatc cccacagcag tttcatgacc ccggagatgt
     9181 acaaggaact ggagcttgag acccggggca gtttcggcgg gatcggcatc gaaataacca
     9241 tccataagga cgtcctcacc gttgtttccc ccatcgaaga caccccggcc ttccaggcgg
     9301 gcgtcaaatc cggagacaag atcatgatga tcgacgggaa atccaccaag gacataaaca
     9361 tcatggacgc ggtgaaacgc ctccggggcg agaaagggac gaaggtaacc atttccgtat
     9421 tgcggggcaa tgaaaacaag ctccgtgaat tcgtcatcac ccgggcgatc atcaaggtga
     9481 tcagcgtcaa acacaaattg ctcgacggcg gaatcggcta tatccgggtc tcctctttcc
     9541 aggacaagac cgccgacgac ctgaaaaaag ccctccggga gatgacggcg aaaaaagagc
     9601 cgctgaaggg gattgtcctg gatttgcgca acaatcccgg cggccttctg aatcaatcca
     9661 tcgaggtctc ggacatattt ctcaagtccg gtggcatcgt ctcaaccaag ggacgctcca
     9721 agggaatgga gagcaaggtc agcgcccggg acaagggcga cgagttcacc gcccccatga
     9781 tcgtcatggt caacgagggg acggccagcg ccgccgagat cgtttccgga gccctccagg
     9841 ataacggccg cgccctgatc ctgggcaccc agaccttcgg caagggctcc gtacagacgg
     9901 ttatccccct ggaagacggc tcggcgttga agttgaccac ggcccggtac tataccccca
     9961 agggacgttc catccaggcg gaaggcatcg ccccggatgt gaccgtcaag tatatccgcc
    10021 cggggagcaa cggcgaggac gaagatcacg agccaattcg agaaaaggat ttgaaaggac
    10081 atatcaaatc cgcccaggag caggcaaagc ccggggaaga ggcggaccgg gaaggagaac
    10141 tgctccagaa gcgcttgaaa aacagcgaac tccgtaaaga tctgccgctc aaaagacctt
    10201 tcggcgatgt caacgcggac aaccagctca aggccgccgt ggacatgctg aagagctggg
    10261 acattttcaa gaagaacctc gtcaaaaact gaaccgggac accatgaaca ctattgccaa
    10321 ggccgccgtt tatctcgctt tcttgtcgat gatccttggc gcggcgccgg acctgtacgg
    10381 aaaaagccgt ccggattatc tggccgccta tcattattcc ttgggcgtct tgcacatgct
    10441 cgacgaaaac ccgcaggagg cgatcaagga atttgaaaag gcccggcgct acgatccggc
    10501 ggcaccggaa atcgccatcg atctggcggc cgcctatgtg gaaaaagggg aagccgccaa
    10561 ggcgatccac ctctgcgaac aggtccggaa ggatcatccg gaccatgtgg atctgaacct
    10621 catcctggcc ggcctgtata tgaacaacaa aaattatccc cgggccgtgg agatctacaa
    10681 acaggtggtc caccaggagc cccggaacgt ctccgcaccg ttttacctgg gggtcatata
    10741 cggggatgcc aagcaatacc cggaagcgat cgccatgttc gaacaggtgc tgcgcctgga
    10801 cccccagcat ctcatggccg cctactacga agcccgggtg ttcatggaga tgaagaatta
    10861 ccagggcgcg gaaaaaatgc tcaaaaagac gctggaaatc cggccgaact tcgaggctgc
    10921 gctgattgat ctggccctgg cgtatgaaaa acagcggcat acttccttgg ccatcgacac
    10981 cttcaaggat tatctcttcc tccatcccga caagctccat gtccgcatca aactcgggga
    11041 aatgtatctg cgggaaaagc gatacgcgga ggcggaaaaa gaattccagc gcatcctccg
    11101 ctcggatccc gataaccagg aggtcctgct caccctcggg ttgcttttca tcgagagcgg
    11161 gcgttacgac caggcggcgg ccaccttcga aaaactggtc cggagatacc ccgccgatca
    11221 acgctacgcc tatctccagg cgacggctta cgaggaaaaa agactctacg ccccggcact
    11281 cgccatcatg gagaagattc cccccacctc ggatctatac agcagcgccc ggatccgtat
    11341 cgccatgatc aggaagaagg aggggaaaat ttcggacgcg atcattgttc tcgaacaggc
    11401 catcaaggtg aaaagggatg tccccggctt ttacgtggtt ctatcctcgc tgtacgaaga
    11461 tcaacaggat cgggtggggg cggaacggat actcaaggag gggatggtga tactgccgga
    11521 gaataccgat ctgcgctaca gcctgggggt gctgtacgaa aagacgggac acttcgaagc
    11581 gagcattcag cagatgcgga ccatcctgaa gatcgatccc gatcacgccg acgccctgaa
    11641 cttcatcggt tacagttacg ccgaccgggg aatgcatctt ggcgaagcgg aaaaactgat
    11701 cctcaaggcc ttgcagctca aacccggtaa cggctacatc atcgacagcc tcggctggac
    11761 ctacttcaag cagaaccgga tcgatctggc cctcaagtat ctccaggagg cggcggccat
    11821 cctccctgga gacggcgcca tcgccgagca tctcggcgac gcctatctta aagccggtcg
    11881 gcggcaggag gccctcaccg cctatcggcg ggcgttgaag atcaatccgg ccagcaccac
    11941 cctgccccag aagatcgaca ccctgacgga taaatgagca ggatccgaat cttagtttta
    12001 ttcctgttct tttctcttat tgccggaggc tgtgcccacg agacgggaac ttcaaaaccg
    12061 ggggaagcaa tcaacctaag tccggaagag gcattggccc gcctcgacct tcggtcgcag
    12121 gataggcgtc tcctcaaagc catagcggaa atcgcggtaa tcacggagac gggacggtat
    12181 cccctcaagc tcgccatcct ggcctggaga ccggattctc tacggctcga ggccattccg
    12241 gtgatcggtt tacccaattt cttcctggcg gcccacgctg ggcggctcgc cgtgtatctg
    12301 cccggaacag gggaatacta caccggcgcg gccgttccgg aaaacctcgc ccgcttcttt
    12361 cccctctcac tgtccatcag cgacctgatt tcgttgttga cgggcggccg tccggcattg
    12421 ccgatggagg agcagccgcg ggtggaagga tttcacgaag gggcgtctta ccgcctcgac
    12481 atctcctccc gggaccgccg attccggcaa tcgctctgga tcgacgggaa aacgggttat
    12541 ctgcgtcgct gcaagcagac ggccgggaac tgcggagatg atgccctgaa cgtatatttc
    12601 gacgcctacg tcccggtgga cggcgtgcct atgccccaga ggatccgggt caaaatgaag
    12661 ggggaggagg agcgaaccgt ggttcttgag tacgaaagcg taacactgga aacgatgacc
    12721 gccgccgggg cggagttgtt tg
//
LOCUS       DAOSMF010000047        12673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_5762_length_12673_cov_21.645348, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000047 DAOSMF010000000
VERSION     DAOSMF010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 12673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12673
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5762_length_12673_cov_21.645348"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="PLT63_06230"
     CDS             <1..210
                     /locus_tag="PLT63_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98356.1"
                     /translation="NNPDIITELLKRYNGHVYFHHNYWCSTAGHHKELCSMMREKYEL
                     TEIAAAQEQHVHYGLYRIRFKENPH"
     gene            complement(503..2347)
                     /locus_tag="PLT63_06235"
     CDS             complement(503..2347)
                     /locus_tag="PLT63_06235"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOD98357.1"
                     /translation="MKLILFNPPAPDGRGFTREGRCTQEAGVWASQWPPVTLATAAAF
                     LAGDGHEVHGVDFPALGLPVRDLEARIAALRPDAALWTTGTPTLAHDLYIATLVKAAS
                     PETLTGVMGTHVSVMPGEVLENSDVDVVIRGEPEGIIRELCRRGNGEGRITRTPGAIA
                     GISHEAGGGGPITLNPEGGGGASASNNRSEGESSAPDRAGVVGTAGGGVRGGIGGGGS
                     GGGRRERWRLSDVVGISYKDAAGAVIHNPDAGWLPPEEIPAPAWEHFDLAPYRLPLSG
                     RPFLIVAPVRGCPYACSFCTAPVYYGRKLRKRPVANVIAEIKDNIRRFGVTEFFIWAD
                     TFTADRKYVRSFCEGILAAGLRITWTCNSRVDTIDGETLALMKRAGLWMISFGLEAGD
                     DGILAASGKRITTADSRRAVHLARAAGVRTAGHFILGLPGETEATMAATLRLACELPL
                     NIAQFYAAAPFPGTRLYEEATVRGWLKTGGGDGNACGNTWGRAADKEADSSSAHEGES
                     MAAPGPEKNASTTEGESTAPPRIPAGPGAGIPPAGDGGGQFSPFSQNSAVLELPGLSA
                     ATVDAFRRRSYRAFYRRPKIIAGLLAMVEPGAAVHLTAAARRFLKWSR"
     gene            complement(2468..3169)
                     /locus_tag="PLT63_06240"
     CDS             complement(2468..3169)
                     /locus_tag="PLT63_06240"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOD98358.1"
                     /translation="MVEETTKFPELTVVIPALNEGSTIETIITGCLRYTDDVVVVDGH
                     SRDDTVRVATARGARIVMDNRRGKGEAIRCVISHLRRPITVFIDADGSHDPDDIPALI
                     APIREGKADHVSGSRLIGGSSELHGGFDECFRLMGSSLITACVNHRFGVQLSESQNGF
                     RAIRTEVLRDLGLVEDITTIEQEMIIKTLKKGYRMGEVPSHEHKRKGGVSKISLRKVA
                     VRYVWTMVKYLYLTK"
     gene            3393..4709
                     /locus_tag="PLT63_06245"
     CDS             3393..4709
                     /locus_tag="PLT63_06245"
                     /inference="COORDINATES: protein motif:HMM:NF021589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOD98359.1"
                     /translation="MRKKQFYSAVATALIFMAVTVGLSWAQDFTVMEKPSGEPRINIG
                     NLTIIPGLQGVMTYDDNIYKGNGKTYANAKTTREEKVVSDMIYHLKPGIMANFIIPER
                     GGITAGWTLDRAWYNTETNNNFQNNGGFLNFDYKMPEGILIGLKDTYSYNTDPYGNAD
                     QYNVGTLTERWSNEFNAKLGYTIGGNFRPMLYYNYFIQQYNNTMKDWAQNYGQNQFGA
                     GLQYRVMPNTWAFVRYVYTQKSYIDNLDPTVTKGDSKTNAAQFGFAWDSGAKLSGEFN
                     FGYQWRRYDNEYVNVNNTGVRRDESNSWSAATNVNFIPMEKTVLGLSIWRTLRDSGSN
                     TNENFWDSGVGLNLSHNFFTKFTFKLGGSFAANDYNRPPAGSPTNVILDKKRQDSNWS
                     FNTGLDYAVQDWLGCGVNYALVTKNSNYEENDYVDNQLSVNLKIVY"
     gene            4729..6114
                     /locus_tag="PLT63_06250"
     CDS             4729..6114
                     /locus_tag="PLT63_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98360.1"
                     /translation="MIALKKQMYVKELFGKRRRQATLAFFSMTLIALMIAFAAGGAEA
                     AIAKITGVKGAVIVNQAGNVSDVTAPGLALNDGDQLLTKEGEAQVTFNDGAILKMQPY
                     TKTMIQERKETRGTLFKATENARRLTCYTGKLWFKSGASKTKNYLQSPTAVAGLRGTD
                     ADFGFNPERMQTLLNMYSGQAAVIGNVIRGFFQNPGVSAAQKSQVYQNLEKAYAVTVE
                     TKKANENPALTQEQKTINTAAAKVEALQVVEQAAQILVQTNPDPVVKTQAQATSQVVT
                     ATITTVKIQEQVAKTEIIRQAAVQEVQKAQAAGDTQKAAEAQKVVQQATVAVRQEQQA
                     VQQAEQRVQQATTLLAAGNVTAVAAQVQQVQRIEQQVQQQAQQLQQVAQQVVTTVATT
                     LPTTIATTIATTVAATGETTAATTAATTTATTAATTAETTTTSVATTIPLPTSSTSSS
                     TSSFATTTSPK"
     gene            6299..7417
                     /locus_tag="PLT63_06255"
     CDS             6299..7417
                     /locus_tag="PLT63_06255"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HOD98361.1"
                     /translation="MRHLNEKALKRLEIILFIAGAMLLLIACAAGGPVVTVTELGDSS
                     KALRNIDLKKGDKAKLEELKKTIIATPRDPGAAALTDILEKSPSYTVAEYLEKYPEGK
                     DTTSDYKIGGYDVLSIQVYEEKDLSRESVRVTADGYITFPLIGRLKVAEKTTSEIEKL
                     IAKELAAKDYLLDAHVSVMVAKYEGRKFSVLGAVTSPGLYPLQAQERVLDGISKAGGI
                     AIARSTDAKQQEAQAQEGMVIRTLHPGKDDEKKIAITFDLQGLLKGKDQQANILLMEK
                     DVVYIPSADYFYIIGEVKTPGSYAFNKKDITIAEAVSMAGGFTRIAARNSTRIVRVEK
                     GVEKIYKVNMDAITKSGKMTQAVFIRPNDLIVVPESFF"
     gene            7552..9969
                     /locus_tag="PLT63_06260"
     CDS             7552..9969
                     /locus_tag="PLT63_06260"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HOD98362.1"
                     /translation="MEIGQKQVNLQEYIRVLMKHRWTILTVFAVIVVSVTIFSFTATP
                     IYQATTRIVIEKENPKVVSIQEVMSVDSSGLDYYQTQYKILESKSVANEVIKRLNLEN
                     NETFNPKPGVIAQVVEWILSPIAYVKSLMKTTDKDAALMGQPGIAEPTSPLVGKLLNA
                     VKVTPIRNSRLVDVSFESPDPVLAARGANTLAKAYIDLNLQTKLKATQDAVNWLNQQI
                     DQEKKKVDTADQALLRYKQEQGIVTDFSTNAETITAQKLAELNKQVVEATSRRVEAET
                     RYRQAQSLAGRADSAASIPEVMSNPLIQEIKKQEVEISKRLSELSKRYGSNHPQIIAL
                     NNEMRTLNARKAAEIQRVVDSLHNQYRVALAGEQSLKGALGQQKGETLAMNQKAIDYT
                     VLKREAETSREMFDLLVKRFKEASLTEDIRTGNIRVIDPAQIPKGPVKPKKLQNILLA
                     IIVGLSLGVGLAFFLEYIDNTIKLPDEVTGILKIPYLGPVPAIAAVAAKEGGGNPGGG
                     GDSGGGGDGSGGVAGAISGVLGNVLGGAISDVGKGARGAAGPDADPTQDIVVVHSPKS
                     TASEAYRGIRTSLLFSSADVAPQIILVTSSGPREGKTTCSANIAAAMAQAGSRVIVVD
                     CDLRRPRVHQLFGRDRSVGTSNILVANCTIDEAIQQTDIPNVDMIASGPVPPNPSELL
                     GSKHMMEMLVELRGRYERIIIDSPPISAVTDAVILSKIVDGVVLVIRAHQTNREVIRY
                     AITQLRNVSAHIFGAVLNGVDMGRDSYYYYQYYYYYYGQDGERKKKTRSRRSLRSRRY
                     YGKDAEK"
     gene            10068..10886
                     /locus_tag="PLT63_06265"
     CDS             10068..10886
                     /locus_tag="PLT63_06265"
                     /inference="COORDINATES: protein motif:HMM:NF039343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98363.1"
                     /translation="MIDLHSHILPELDDGATSWEQALEMARVAVADGVTEVVCTPHWV
                     PGKFDNSRPVILEQVETCRRLLADAQIPLKVHPGAELRIDINLPGLIKNRQVLTVNDG
                     GVYALIELPDESIPDHLDEFFWNLELLRIRPIISHVERNAVLREEPHRLYHWAVNGYL
                     AQITASSLTEHFSEEIQDFAVFILEHRLAQMLVSDGHGLRSRSPRLSAGLAVAAQILG
                     AQTARRMVWDVPRQILNGEAVRFPEPVPIDEPRRIDIFDYLPKRFGDKIKSLLK"
     gene            11126..11452
                     /locus_tag="PLT63_06270"
     CDS             11126..11452
                     /locus_tag="PLT63_06270"
                     /inference="COORDINATES: protein motif:HMM:TIGR04176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family EPS-associated transcriptional
                     regulator"
                     /protein_id="HOD98364.1"
                     /translation="MKATIEKTQILRDLARNPYISQRELAERHRISLGKVNYAIRALI
                     KKGYIKLQNFYKSNNKRKYAYVLTPEGMHEKTKLTASFLSWKMKEYERIKQEIADLKQ
                     ELDASE"
     gene            11685..>12673
                     /locus_tag="PLT63_06275"
     CDS             11685..>12673
                     /locus_tag="PLT63_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98365.1"
                     /translation="MTKAPVKNDQQVSVSPSGEVFPLPTPADYNKELKRLQTLVKKHR
                     REGREIVVVMGVGFVGAVMAGVVADSVWKESGVRSLKLASGGDFELKSQTPGNIAENF
                     GKGVSETSQAAATAATATTAAATSAAATAAATSAASAATTATAATAASSSPSSAPVKN
                     ANFKDLTPGFPNKFVIGMQRPSPRSYWKIPLLNRGIAPVESEDPEVAPLIARCVLEKK
                     TLTATFTYDALSLADVVVVDVQCDYFKETLGNCRQGHADIAALEESLKVIGERITPDC
                     LVLIETTVPPGTTEYVAYPIIKKAFEKRGLMMPAAAAATAPIAATAATAPTATA"
BASE COUNT         2709 a         4109 c         3728 g         2127 t
ORIGIN      
        1 aacaatcccg acatcatcac cgaactcctg aagcgctaca acggccacgt gtacttccac
       61 cacaactact ggtgcagcac cgccggccat cataaggagc tgtgctccat gatgagggaa
      121 aagtacgagc tgacggaaat cgccgcggct caggagcagc acgtccacta cggcctgtac
      181 cggatccggt tcaaagaaaa tccgcactga atccggctgc agccggcgcc cggacggcaa
      241 gaccgagaaa cttcccaaag agaaccgccc ccattgtgag aaccccatcc ccacctcaac
      301 cctccctttg aaggagaggg agctgcaagg gggaggcggt tgcaaaggga agaagctgcg
      361 ggaagacggg tttttaaatt ttgctaatct cttcgggtgc aattccccga agcttgatga
      421 actcgtaaaa agtcatcaag cttgctttga catggggaat ttttacctgt gatacctcgc
      481 agcttgctgc gaggcggttc atttaccggg accatttgag gaagcgccgg gcggcggcgg
      541 tgaggtggac ggcggcgccg ggctccacca tggcgaggag tccggcgata atcttcgggc
      601 ggcggtagaa ggcgcggtag gaccggcggc ggaaggcgtc cacggtggcg gcggagaggc
      661 cgggcagttc gaggacggcg ctgttctggg aaaacgggga gaattggccg ccgccgtcgc
      721 cggccggtgg gatgccggcc ccgggaccgg cggggatacg gggcggcgct gttgattcgc
      781 cttcagtcgt cgaggcgttt ttttccggtc ctggtgccgc cattgattcg ccttcgtgcg
      841 cggaggaaga atccgcttcc ttgtcagcgg cgcggcccca agtgttgccg caggcgttgc
      901 cgtcgccgcc ccccgtcttc agccagcccc taacggttgc ctcctcgtag aggcgggtgc
      961 cggggaaggg agcggcggcg tagaactggg cgatgttcag gggcagctcg caggccaggc
     1021 gcaatgtggc ggccatcgtc gcttccgtct cgccggggag tccgaggatg aagtgccccg
     1081 ccgtccgcac ccccgccgcc cgggccaggt gcaccgcccg ccgggaatcc gccgtggtga
     1141 tcctctttcc cgacgccgcc aggatgccgt cgtcccccgc ttccaagccg aaactgatca
     1201 tccacagccc cgcccttttc atcagcgcca gggtctcgcc gtcaatggtg tccacccggc
     1261 tgttgcaggt ccaggtgatc cggagccccg ccgccagaat cccctcgcag aagctccgga
     1321 catacttccg gtccgccgtg aaggtgtcgg cccagatgaa gaattccgtc acgccgaagc
     1381 gccggatatt gtccttgatt tccgcgatga cgttcgccac gggccgcttg cggagcttcc
     1441 ggccgtaata gaccggagcc gtgcagaagc tgcatgcata ggggcagccc cgcacggggg
     1501 cgacgatgag aaacggccgc ccggaaaggg gaaggcggta aggcgccaga tcgaaatgtt
     1561 cccatgccgg tgcgggaatc tcctccgggg gcagccaccc ggcatcggga ttgtggatga
     1621 cggcgcccgc cgcatcctta tatgatatgc ccacaacatc ggacagccgc cagcgttccc
     1681 gccgtccgcc gccgctcccg ccgccgccaa tgccaccacg aacgccgccg ccggcagtgc
     1741 cgacaacccc ggcgcggtcg ggggcgctgc tttctccctc gctgcggtta ttactggccg
     1801 aggcgccgcc gcctccctcg ggattgaggg tgatggggcc gcctccgccc gcctcatgtg
     1861 atatgcccgc gatcgcgccg ggtgttcggg tgattcgccc ctcaccgttt ccccggcggc
     1921 agagttcccg gataattccc tccggctccc ctctgatcac cacgtccacg tcggaattct
     1981 ccaggacttc cccgggcatg acgctgacgt gggtccccat gacccccgtg agggtctccg
     2041 gggatgccgc cttcacgagg gtggcgatgt acaggtcatg ggccagggtg ggagtgccgg
     2101 tggtccagag ggcggcgtcg gggcgcaaag cggcgatccg ggcctccagg tcccggacgg
     2161 gaagccccag ggcggggaaa tcgacgccgt gcacctcatg gccgtcgccg gcgagaaagg
     2221 ccgccgccgt cgccagggtc accggcggcc actgggaggc ccagaccccc gcctcttggg
     2281 tgcagcgccc ctcccgggtg aatccccggc cgtccggcgc cgggggattg aataagatca
     2341 atttcatgac cggtaataac cccatccccc ttctatgcct ccccctttta agaaggggag
     2401 gaatagaaag aaatcagaac cgtcccgatt tttacaaaac ttttacagga atcagaacct
     2461 gccccgttta tttggtgagg tacaggtact tgaccattgt ccatacgtag cggacggcga
     2521 ccttgcggag gctgatcttg gagacgccgc ccttgcgctt gtgctcgtgg gacggcactt
     2581 cgcccatgcg gtagcccttc ttcaaggtct tgatgatcat ctcctgctcg atggtggtga
     2641 tgtcctccac cagccccagg tctcgcagga cctccgtccg gatggcccgg aagccgttct
     2701 ggctctccga gagctggact ccgaagcggt gattgacaca ggcggtgatg agggaactgc
     2761 ccatgaggcg gaagcactcg tcgaagccgc cgtggagttc gctggagccg ccgatgagcc
     2821 gggagcccga cacgtggtcg gccttgccct cccggatcgg ggcgatgagg gcggggatgt
     2881 cgtcgggatc gtgggagccg tcggcgtcta tgaagaccgt tatgggccgc cgcaggtggg
     2941 agatgacgca tctgatggcc tcgcccttgc cccggcggtt gtccatgacg atccgcgccc
     3001 ccctcgccgt ggccacgcgg acggtatcgt cccgggagtg gccgtccacc accaccacgt
     3061 cgtcggtata acgcaggcag cctgttatta ttgtttcgat ggtggaccct tcgttcaggg
     3121 ccggaatcac caccgtaagc tcaggaaatt tcgttgtctc ttcaaccatc ggcattatga
     3181 tcctcgattt gaatgatctc cccgcataaa aaactatgaa aaaactcttg acatccgggg
     3241 gcgatttcaa tagcatcata gccgctctgt gtaaatctat ttgttgtttc ttaagcgttt
     3301 tctcggagaa ttcgcggtgt tgagcttgct tttcgggtaa gccggaagtc gtatgtcgtg
     3361 atattatttt gtttgttatg gagggaggca ttatgcgaaa aaagcaattc tattcggcgg
     3421 ttgcgacagc cctgatcttc atggcggtaa cggtgggtct ctcatgggcc caggatttca
     3481 ccgtcatgga aaagccgagt ggcgaaccca ggatcaatat cggcaatctc accatcatcc
     3541 cgggcctgca aggcgtgatg acatatgacg acaacatcta caagggcaac ggcaagacct
     3601 acgccaacgc caagacgacg agagaggaga aggtcgtctc cgacatgatc tatcacctca
     3661 aacccggcat catggccaac ttcatcattc cggaacgggg cgggatcacc gccggctgga
     3721 ccctggaccg ggcctggtac aacacggaga ccaacaacaa cttccagaac aacggcggtt
     3781 tcctcaactt cgactacaag atgcccgaag gcatcctcat cggccttaaa gacacctact
     3841 cctacaacac cgacccctat ggtaatgccg accagtacaa cgtcggcacc ctcaccgagc
     3901 gctggtccaa cgaattcaac gccaagctcg gctacaccat cggcggcaat ttccgcccca
     3961 tgctctacta caactacttc attcagcagt acaacaacac catgaaggac tgggctcaga
     4021 actacggcca gaaccagttc ggcgccggcc tccagtaccg cgtcatgccc aatacctggg
     4081 cgttcgtccg gtacgtttac acccagaagt cctacatcga caacctcgat cccacggtca
     4141 ccaagggcga ctccaagacc aacgccgccc agttcggatt cgcctgggat tccggggcca
     4201 agctcagcgg cgagttcaac ttcggttatc agtggcgccg ttacgacaac gaatacgtga
     4261 acgtcaacaa caccggcgtc cgccgcgacg aatccaactc ctggtccgcc gccacgaacg
     4321 tcaacttcat ccccatggaa aagacggttc tcggcctgag catctggagg acccttcgcg
     4381 actccggctc caacaccaac gagaacttct gggacagcgg cgtcggcctc aacctgagcc
     4441 acaacttctt cacgaaattc accttcaaac tcggcggcag cttcgcagcc aacgactaca
     4501 accggccgcc ggcgggcagc cccaccaatg tgattctcga caagaagcgg caggatagca
     4561 actggagctt caacaccggt ctcgactacg ccgttcagga ctggcttggt tgcggggtta
     4621 attatgccct cgtgacgaag aactccaatt atgaagaaaa tgattatgtg gacaaccagc
     4681 tcagtgtcaa cctcaagatc gtttactaag ggaaggggag ggacaatcat gattgccttg
     4741 aaaaaacaga tgtacgtaaa agagttgttc ggtaagcgga ggcgtcaggc gaccttagcg
     4801 ttcttctcca tgaccctgat cgccttgatg atcgcctttg cggccggcgg cgccgaggcc
     4861 gccatcgcca agatcaccgg cgtcaagggt gccgtgatcg ttaatcaggc gggtaacgtc
     4921 tccgacgtca ccgctcccgg cctggcattg aacgacggcg atcagctcct caccaaagag
     4981 ggggaggccc aggtaacctt caatgacggc gcgattctca agatgcagcc ctataccaag
     5041 accatgatcc aggaacggaa ggagaccagg gggacgctct tcaaggccac ggagaacgcc
     5101 cggcgtctca cctgctatac cggtaagctc tggttcaaat ccggggcctc caagaccaag
     5161 aattacctcc agtcgcccac ggccgtcgcc ggcctgaggg ggacggacgc cgatttcgga
     5221 ttcaatcccg agcggatgca gaccctcctc aacatgtaca gcggacaggc cgccgtcatc
     5281 ggcaacgtta tccggggctt cttccagaac cccggcgtct cggcggccca gaagagccag
     5341 gtttaccaga acctggagaa ggcctacgcc gtcaccgtgg agacgaagaa ggccaacgaa
     5401 aaccccgccc tgacccagga gcagaagacg atcaacaccg cggcggcaaa ggtggaggcc
     5461 ctgcaggtcg ttgagcaggc cgcccagatc ctcgttcaga ccaaccccga tccggtggtc
     5521 aagacccagg cccaggccac gtcccaggtg gtgaccgcca ccattaccac cgtgaagatc
     5581 caggagcagg tcgccaagac ggagatcatc cgccaggcgg cggtccagga ggtccagaag
     5641 gcccaggcgg cgggcgatac ccagaaggcc gcggaggccc agaaagtggt ccagcaggcc
     5701 acggtcgccg tgcggcagga gcagcaggcg gttcagcagg cggagcagcg ggtccagcag
     5761 gccacgaccc tcctggcggc gggcaacgtg accgcggtgg cggcccaggt ccagcaggtc
     5821 cagcggatcg agcagcaggt ccagcagcag gcgcagcaac tccagcaggt ggcgcagcag
     5881 gtggtcacca cggtggcgac gacgctgccg accaccatcg ccacgacgat cgccaccacc
     5941 gtagccgcta cgggggaaac gaccgcggcg acgacggcgg ccacaaccac ggccaccacg
     6001 gcggcgacga cggcggaaac caccactacg agcgtggcga ccaccatccc gctgcccact
     6061 tcctccacct cgtcctcgac ctcttcgttt gccacgacca cgagcccgaa gtaacggcat
     6121 tgtgaagttt tattgacgct gaacattttt cccccccttc aaaaaagggg gggaaatttt
     6181 ttcgtaaatg aggtaaacgg gggtgccccg aagcaggcgg tccggatggt tgaggcaatg
     6241 accaaggacg cggatgcgcc gcggaccggg gatgcctcaa gacttttggg aagtgaagat
     6301 gcgacaccta aacgaaaaag cactaaaacg actggaaatc attcttttta ttgccggtgc
     6361 gatgctcctg ctgatcgcct gtgccgccgg cgggcccgtc gtcacggtga cggagttggg
     6421 cgattcatcc aaggccctga ggaacatcga cctgaagaag ggcgacaagg ccaaactgga
     6481 ggagctgaaa aagaccatca tcgccacccc ccgggatccc ggcgccgccg ccctgacgga
     6541 catcctggaa aagagcccct cctacaccgt cgccgaatat ctggagaaat atcccgaggg
     6601 caaggacacg acgagcgact acaagatcgg cggctacgac gtcctgagca tccaggttta
     6661 cgaagagaag gacctctccc gggaatccgt gcgggtcacc gccgacggct acatcacctt
     6721 tcccctgatc gggaggctta aggtggcgga gaagaccacg tcggagatcg agaagctcat
     6781 cgcgaaggaa ctggcggcga aggactatct tctggacgcc cacgtctccg tcatggtggc
     6841 gaaatacgaa ggccggaagt tctccgtcct cggagcggtg acgagtcccg ggctctaccc
     6901 ccttcaggcc caggagcggg tcctcgacgg gatatccaag gccgggggca tagccatcgc
     6961 ccgctccacc gacgccaagc agcaggaggc ccaggcccag gagggaatgg tcatcaggac
     7021 cctgcatccg gggaaggacg acgagaagaa gatcgccatc accttcgacc tccaggggct
     7081 gctcaaggga aaggaccagc aggccaacat cctcctcatg gagaaggacg tggtctatat
     7141 ccccagcgcg gactacttct acatcatcgg cgaggtcaag accccgggct cctacgcctt
     7201 caacaagaag gacatcacca tcgccgaggc ggtgagcatg gcggggggct tcacccgtat
     7261 cgccgcccgg aacagcaccc ggatcgtccg ggtggagaag ggcgtggaga agatctacaa
     7321 ggtcaacatg gacgccatca ccaaatccgg caagatgacc caggcggtct tcatccgccc
     7381 caacgacctc attgtcgtgc cggagagctt tttctaatcg cgtggcgtgg cctccgatcc
     7441 ttatgcccct tccccttgcc ggggggaggg taaggggaag gggagacagg gatgtatttt
     7501 cgtacaatac ttttctttaa attctgctga acgggagtaa gcgccaaacc tatggaaatc
     7561 ggtcaaaaac aggtaaatct tcaggaatat atccgggtgc tcatgaagca ccgctggacg
     7621 atcctcacgg tcttcgccgt gatcgtggtg agtgtgacga tcttttcctt cacggcgacg
     7681 cccatctacc aggcaaccac ccgcatcgtc atcgagaagg aaaaccccaa ggtcgtatcc
     7741 atccaggagg tcatgtccgt ggactcctcg ggcctggact actaccagac ccagtacaag
     7801 atcctcgaga gcaagtcggt ggccaacgag gtgatcaagc gcctcaattt ggaaaacaac
     7861 gagaccttca accccaaacc cggcgtgatc gcccaggtcg tggaatggat cctttccccc
     7921 atcgcctatg tcaagtccct catgaaaacc acggacaagg acgccgccct gatggggcag
     7981 cccgggatcg ccgagcccac ctcgccgctg gtggggaagt tgctcaacgc tgtcaaggtc
     8041 acgcctatcc ggaacagccg cctggtggac gtgagcttcg aatccccgga cccagtcctg
     8101 gcggcccggg gggcaaacac cctggcgaag gcctatatcg acctcaacct ccagacgaag
     8161 ctcaaggcca cccaggacgc ggtgaactgg ctaaatcagc agatcgacca ggaaaagaag
     8221 aaggtggaca cggcggacca ggccctcctg cgctacaagc aggagcaggg gatcgtcacg
     8281 gacttctcca ccaacgccga aacgatcacc gcccagaagc tggcggagct gaacaagcag
     8341 gtggtcgagg cgacctcgag gcgcgtcgag gcggagaccc ggtaccgcca ggcccaatcc
     8401 ctggccggcc gggccgacag cgcggcctcc atacccgagg tcatgagcaa ccccctgatc
     8461 caggagatca agaagcagga ggtggagatc tccaagcgcc tctccgagct ctcgaagcgc
     8521 tacgggagca accaccccca gatcatcgcc ctgaacaacg agatgcggac cctcaacgcc
     8581 cgcaaggcgg cggagatcca gcgggtggtg gattccctcc ataaccagta ccgcgtcgcc
     8641 ctggccgggg agcagtccct caagggcgcc ctggggcagc agaaggggga gacgctggcc
     8701 atgaaccaga aggccatcga ctacaccgtc ctgaaacggg aggcggagac gagccgggag
     8761 atgttcgatc tcctggtgaa gcgcttcaag gaggcctccc tcaccgaaga catccgcacc
     8821 ggcaacatcc gggtcatcga tcccgcccag atccccaagg ggcccgtgaa gcccaagaag
     8881 ctccagaaca tcctgctggc catcatcgtc ggcctgtcgc tgggcgtcgg cctggccttc
     8941 ttcctggagt acatcgacaa caccatcaaa ctccccgacg aggtcacggg gatactgaag
     9001 atcccctacc tgggtcccgt gcccgccatc gccgccgtgg ccgccaagga gggcggcgga
     9061 aacccgggcg gcgggggcga ttccggtggc gggggcgacg ggagcggcgg agtcgccggc
     9121 gccatcagcg gcgtactcgg caacgtcctg ggtggcgcca tcagcgacgt cgggaagggc
     9181 gcccgaggcg cggcggggcc cgatgccgac cccacccagg acatcgtcgt cgtccactcg
     9241 ccgaaatcca cggccagcga ggcctaccgg gggatccgga ccagcctgct gttctcctcc
     9301 gccgacgtgg ccccccagat catcctggtc accagctccg gcccccggga ggggaagacg
     9361 acctgctccg ccaatatcgc cgccgccatg gcccaggcgg ggagccgggt gatcgtcgtg
     9421 gactgtgacc tccgccgccc ccgggtccat cagctcttcg ggagggaccg ctccgtgggg
     9481 acctccaaca tcctggtggc gaactgcacc atcgacgagg ccatccagca gacggacatc
     9541 cccaatgtgg acatgatcgc ctccggacca gttccgccca acccatcgga attgctgggt
     9601 tccaagcaca tgatggagat gctggtggag ctgcgtgggc gttacgagcg gatcatcatc
     9661 gattcgccgc ccatctccgc tgtcaccgac gccgtcatcc tctccaagat cgtcgatggc
     9721 gtggtcctgg tcatccgggc ccaccagacc aaccgggagg tcatccggta cgccatcacc
     9781 cagctccgga acgtcagcgc gcatatcttc ggggcggtcc tcaacggcgt cgatatgggc
     9841 cgtgacagct actattacta ccagtactac tattactact acggccagga cggcgagcgc
     9901 aaaaagaaga cccgcagccg ccgctccctc cgcagccgcc gctactacgg caaagacgca
     9961 gaaaaatagg gggtcaggtc tttaaaatta gcgtttttgg ataatcatta attcggggca
    10021 tcgggggtat cgccccattt accctaaatg atagaaaggc cgccgcgatg atcgaccttc
    10081 acagccacat tttaccagaa ctggacgacg gcgccacctc ctgggaacag gccctggaga
    10141 tggcccgcgt ggccgttgcc gacggcgtca cggaggtggt ctgcaccccc cactgggtgc
    10201 cggggaagtt cgacaacagc cgccccgtca tcctggagca ggtggaaacc tgccggcgcc
    10261 tcctcgcgga tgcccagatt cccctcaagg tccatcccgg cgcggaactc cgcatcgaca
    10321 tcaacctacc cgggctgatc aagaaccggc aggtcctgac cgtcaacgac ggcggggttt
    10381 acgccctcat cgagttgccc gacgaatcca ttcccgatca cctggacgag ttcttctgga
    10441 acctggaact gctgcgtatc cgccccatca tcagccatgt ggagcgcaac gccgtcctcc
    10501 gggaggaacc ccaccgcctc taccactggg cggtgaacgg ttacctggcc cagatcacgg
    10561 cctccagcct gacggagcat ttttccgaag aaatccagga ttttgcggta ttcatcctgg
    10621 agcaccgcct cgcccagatg ctggtctccg acggccacgg actacgcagc cgctcgcccc
    10681 gcctttccgc cgggctggcc gtggcggccc agatcctggg ggcgcagacc gcccggcgca
    10741 tggtctggga cgtcccccgg cagatcctca acggcgaggc cgtgcgcttt cccgagcccg
    10801 tccctatcga cgagccccgc cgcatcgaca tcttcgacta cctgccgaaa cgcttcggcg
    10861 acaagatcaa atccctcctg aaataatcag catttcggga caaatccgac atggtattat
    10921 tctttacatg gtctttcttc tatgttatag cccgcccatt gcgtgttcag acttgaacgc
    10981 tgcacaacag gccggcgggc aggacgcctg cacagcgggg cgagcggata gcgcctcaac
    11041 ctccgtcggc gaaacggcgg gaacgccagt ggggccagcc gcggcacagg cagcatcgcc
    11101 atcaaagggg ggagaccggc ggtagatgaa ggccacgatc gagaagacgc agatcctgcg
    11161 ggatctggcc aggaatccct atatatccca gcgggagctt gcggagaggc accgcatcag
    11221 cctcggcaag gtcaactacg ccatccgcgc cctgatcaag aagggataca tcaagctcca
    11281 gaacttctat aaatccaaca acaaacgtaa atatgcatac gtgctgaccc cggaggggat
    11341 gcacgagaag acgaagctca cggcctcctt cctgagctgg aagatgaagg aatacgagcg
    11401 cattaagcag gagatcgccg atctgaagca ggaactggac gcctctgaat aatcgggaat
    11461 aatcggggga catatcactg aataaccaga gccgcgggaa taattagcgg acatgtcact
    11521 gattaaccag aactgctgta ttttacataa ttgatatagg atgccacttt cccactcgcc
    11581 tcatccgaag ccaccccgga tgtaaccgag agggcggagc tgtagcagat ggctgccaac
    11641 cgatcccggt agcttaaaaa cacctgaaag gattcaaccc ccctatgacc aaagcccccg
    11701 ttaagaatga tcagcaagta agcgtctcgc cctccggcga ggtcttcccc ctgcccactc
    11761 ccgcggatta caataaggaa ctcaagcgcc tccagaccct tgtgaagaag catcgccggg
    11821 aaggccggga gatcgtcgtc gtcatgggcg tcggcttcgt cggcgccgtc atggccggcg
    11881 tcgtggccga ctccgtctgg aaagaatcgg gggtcaggtc tttaaaatta gcatctggcg
    11941 gcgattttga attgaaatcc cagaccccag ggaatattgc ggaaaatttc ggaaagggtg
    12001 tttctgaaac ctcccaggcc gccgcgaccg ccgcgaccgc caccaccgcc gcagcaacat
    12061 cagcggccgc caccgccgca gcaacatcag cggccagcgc cgccaccacc gcgaccgccg
    12121 cgaccgccgc ctcctcctct ccgtcctccg ccccggtgaa aaatgctaat tttaaagacc
    12181 tgacccccgg gtttcccaac aaattcgtca tcgggatgca gcggccctcg ccgcgctcct
    12241 actggaagat ccccctcctc aaccgcggga tcgcccccgt ggagtccgag gaccccgagg
    12301 tggcgcccct catcgcccgc tgcgtcctgg agaaaaagac cctcaccgcc accttcacct
    12361 acgacgccct gtcgctggcc gacgtggtcg tcgtcgatgt ccagtgcgac tacttcaagg
    12421 aaaccctcgg caactgccgc caggggcacg ccgacatcgc tgccctcgag gagagcctga
    12481 aggtcatcgg cgagcgcatc accccggatt gcctcgtcct catcgagacc accgtgcccc
    12541 cgggcaccac ggagtacgtc gcctatccca tcatcaagaa ggccttcgag aagcgcggcc
    12601 tgatgatgcc cgcggcagcc gccgccacag cgcccatcgc cgccaccgcc gccacagcgc
    12661 ccaccgccac cgc
//
LOCUS       DAOSMF010000048        12635 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_5789_length_12635_cov_19.732989, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000048 DAOSMF010000000
VERSION     DAOSMF010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 12635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12635
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5789_length_12635_cov_19.732989"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="PLT63_06280"
     CDS             complement(<1..390)
                     /locus_tag="PLT63_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98366.1"
                     /translation="MTIRTRLMLVTVLAIAMTMAVWGWVQLQVFDNLLTEQQAQRLGE
                     LADTVSTYYEYFPTRRGLSALDATLKDHVQTDPRLARIDLLTIEKSAVEFVAGAGRIA
                     YDWPENTIAAAIETMKPQYLSINTEGGP"
     gene            complement(482..877)
                     /locus_tag="PLT63_06285"
     CDS             complement(482..877)
                     /locus_tag="PLT63_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98367.1"
                     /translation="MSLLCIVVATSLAVGQPEPGPAKTATVKTVPIKPAPKKAPPRET
                     RMRATGKVIEISPTALKIERNVKGTVETMDFVLPKPQDKIAVGDEVGVNYITKDHQHI
                     AKRVGKVAKKKVFSPPKGIKSPPPYPAAK"
     gene            1327..2055
                     /locus_tag="PLT63_06290"
     CDS             1327..2055
                     /locus_tag="PLT63_06290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HOD98368.1"
                     /translation="MERKSFKYYDMIMALFVMVVVMSNILSSAKIVDWGISLFGIPLA
                     FDAGTILFPVSYIFGDILTEVYGYRRSRRVIWMGFSCLILSSLLLYFVKIMPGEGIWE
                     KTAGQAAYDAVLSGMTTGGIVAASLLGYWLGEFSNSYVLAKMKILTGGRWLWTRTVGS
                     TLVGEGIDTMVFVSVAVVCGVFPAALFMTLAVTNYLFKVGVEVIMTPATYAAVDALKK
                     AEQEDFYDFRTNFNPFSRGSNIYS"
     gene            2056..2694
                     /locus_tag="PLT63_06295"
     CDS             2056..2694
                     /locus_tag="PLT63_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98369.1"
                     /translation="MKFSICLLSLVSILFIPVPSAGADRDLLTKQVLEQATVKYRTDF
                     AVEAPLEIWDKVLAHPLLMARLWNVYNFQPAYEVKDQKEGVLVIDPSGIKGKLAMTES
                     RPDGRSFYGWGSVDHWAIPSFISAEGIFRFRYTGDGRRVRGTFEAFMKGDNKATDFLM
                     KLVRGKVKNLLHRRFTNNLNDMKKIIADIVQNPDQIRGRLTGGDLKEFNNAF"
     gene            2835..4217
                     /locus_tag="PLT63_06300"
     CDS             2835..4217
                     /locus_tag="PLT63_06300"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOD98370.1"
                     /translation="MTDWKETAGMVIVIGAGAAGMMAAGRAAEAGARVLLLEKTERPG
                     KKILVSGKTRCNLTNTRELQEFIPMYGAAGSFLRAAFHRFFREDLLVLLRRYGVETKA
                     ERGGRIFPVSDRATDVVEAFRRYLDEHGVELVTRQRVLSLVRREGAVTGVMTAGGTLP
                     ADAVIVAAGGATWPATGSTGDGFELARGVGHRIEKLRPALVPLVVRETVLAKSLQGVN
                     LRNIRLTAFRGIPGDFDPAFIPDRDVGRGTGGRKARRPVIESRCGEMMMTHFGIGGPV
                     TLLMSLAVVKALEEGPVSVALDLKPALDYRQLRARLQRDLDAHGKRQCRGILAGLLPR
                     KFVTPLAAQAGIPPDKPANQVTAAERDRLVTVLKSLCFEITSSLPMATAIVTAGGVSL
                     PEVDPRTMASRLATGLYFCGEVLDVDADTGGFNLQAAFSTGWVAGESAAAYAARRHGH
                     APPNPELFLS"
     gene            4266..4997
                     /locus_tag="PLT63_06305"
     CDS             4266..4997
                     /locus_tag="PLT63_06305"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98371.1"
                     /translation="MMEAEEGLMRLGIVAALAAEARILSPGVVPMQTVVPFRGAGLLI
                     LSGIGSGRAAGAARKLIDHGAEALLSWGIAAGLAADLAAGTLVVPETVVDDAGETFAT
                     DRAWRKRVTGRLGAFLTVSNGMLVQGRAMLVTGQDKINLATATGAVAMDMESGAVAAV
                     AREAGIPFLAVRAISDPVGMSIPPGVLAAVDRYGRVRSLRMFLRLARHPGEVMGIVAL
                     GWSFRAACRTLRAAGGDESVFPPAG"
     gene            5028..6017
                     /locus_tag="PLT63_06310"
     CDS             5028..6017
                     /locus_tag="PLT63_06310"
                     /inference="COORDINATES: protein motif:HMM:TIGR03466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOD98372.1"
                     /translation="MKILITGATGFIGGAVLRQLAAAGHDVRALVRPGGDRRNLTGLA
                     VETSVGDLRDLPSLERALRDCRILFHVAADYRLWVPRPAELYETNVAGTRNIVMAAAA
                     AGVERIVYTSSVAVLGLHHDGRAADEDTPVTAADMIGHYKRSKFIAEAEVRKLVTERG
                     APVVIVNPSTPVGPGDVKPTPTGRIIVDAVSGRMPAYVDTGLNFVHVDDVAAGHLLAL
                     ERGRIGERYILGGVNMTLKKALDEIAALSGCRRPRIRLPHGLVLVLAAISEAWSLATR
                     TEPRVNLTGVRLARKKMYFSTAKAQRELGYTIRPVREAFRDAIAWFRQQGYLQ"
     gene            6014..6151
                     /locus_tag="PLT63_06315"
     CDS             6014..6151
                     /locus_tag="PLT63_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98373.1"
                     /translation="MSSPRAAAGAESLRSGAIAREFKMRGGSGSAMAGTGRFGSVPGE
                     A"
     gene            6159..7319
                     /gene="hpnI"
                     /locus_tag="PLT63_06320"
     CDS             6159..7319
                     /gene="hpnI"
                     /locus_tag="PLT63_06320"
                     /inference="COORDINATES: protein motif:HMM:TIGR03472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohopanetetrol glucosamine biosynthesis
                     glycosyltransferase HpnI"
                     /protein_id="HOD98374.1"
                     /translation="MDISIPQALLLIPVIAGSVYEVIRLVIVKRFLAGHMDRDGVPPG
                     GWPPVTILKPLRGLDKGLAENLRSACRQDYPRYQVVLAVQDRDDPALPVMEEIRREFP
                     DRVELAVENVVVGFNGKINNLVGALRHARHDILVISDSDVRLPEDYLRCIVAPLAGPG
                     GGKVAYACTLYRAAGAGAWYEKLGLLTYNAAFIPDIIFAYVTNAAPFCIGASVALRRR
                     DLEAAGGFAALGDYLAEDYEMGRRLLARGGRMVLLPREVVITVDLPSSARWWAGQVSW
                     DLKTRSARPTGFFAAVVTRAIPFAALFAALRLADGVGMAVLVTAIVIRLGTAAALLGV
                     LRDREGRRALWLLPVRDVAGFFSWAAALVSRTVVWRGTRYVLDRHGRLAGVR"
     gene            7373..8245
                     /gene="hpnK"
                     /locus_tag="PLT63_06325"
     CDS             7373..8245
                     /gene="hpnK"
                     /locus_tag="PLT63_06325"
                     /inference="COORDINATES: protein motif:HMM:TIGR03473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid biosynthesis-associated protein HpnK"
                     /protein_id="HOD98375.1"
                     /translation="MRALIITADDFGFSPAVNEAVEEAHRRGVLGTASLMVGAPATRD
                     AVARAVRLPGLKVGLHIVVAGAPPVLSPSEIPALIDPEGKLSSRLFRAGVNFYFRPAV
                     RAQLTREIRAQFELFRGYGLALDHVNCHKHLHLHPTVFALITEIGAAYGLKAVRLPYE
                     PLSGAAPDLLRKGGAGQLAASLFLLPWTGLLKRKIRAAGLRANDFIFGMRDSGRMESA
                     RVLRLLPRLPAGVTEMYFHPAARSCPELTAEAPGARHEAEFAALTDPAFPAALAELGI
                     RRVSFSDLPLNIHA"
     gene            complement(8266..9702)
                     /gene="hpnJ"
                     /locus_tag="PLT63_06330"
     CDS             complement(8266..9702)
                     /gene="hpnJ"
                     /locus_tag="PLT63_06330"
                     /inference="COORDINATES: protein motif:HMM:TIGR03471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid biosynthesis associated radical SAM
                     protein HpnJ"
                     /protein_id="HOD98376.1"
                     /translation="MKTLLLNPPSFAGFDGGAGARYQATREITAFWYPTWLAYPAGLI
                     PESRLLDAPAHGVSPEETVQIAADFAFVVIFTSTAGFANDIRLAELMKTANPALRIAM
                     VGPHVTVLPEQSLRAGTAVDFVVRKEFDHAVAEIARGKPAAEIAGISFREGERIVHNP
                     DRPPLEDLDSLPFAVDIYRRDLDFRQYDIPFIKYPYVSFYTSRGCPAQCTFCLWPQTM
                     SGHRIRTRSIGNVVAELKRAFRHFPEAREIFFDDDTFTWNRQRIMEFCRAFKPLGFTW
                     SCNARVNTDYEVLREMKDAGCRLLVVGFESGDPVILKNIRKGATVEQARELMKNCRRL
                     GLQVHGDFQIGHPGETRETIARTIRFAVELDPDTIQVSISHPFPGTRFYDYLVANGYL
                     VAAEMADAQGHQLPVISYPDLSRDEIVAAVQDFYRRYYYRPRIIFRILRGALGNSDDR
                     RRIVKEGREFLGFRSQSRKNIRHLKRDH"
     gene            complement(9659..12505)
                     /locus_tag="PLT63_06335"
     CDS             complement(9659..12505)
                     /locus_tag="PLT63_06335"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOD98377.1"
                     /translation="MKIKRFVKSALGGLVAFQIKRPWTVMFAVAVISVLAALYTANNL
                     GIQASQSDLISPQEQLIQIARQFHDFEQLDSFIVVIEGPETRALEFLKRLAPRIEADR
                     RNFAQVFYRIDPEALKPWLLLYLDGQDLRRLRDKLAENKRFLQEISRLPTLENFFAQL
                     NHELSSRVVDELFTGFLEDHREGGEPLDPAFLIRVLEEIKRSLAGQGPFNSPWEALFS
                     ERVAGDTSREGYFWTADKRCLLAFVTPVRLANDFTGTKRSLAALREAVAEVRAEFPDI
                     MAGVTGPEALNADQMSAAMTDMELATVLSLAGLTVLFIIFRRSIRRPVFEMITLVVAL
                     TWCFGLTTLAVGHLNILSITFAPLILGLGIDNGAHWFARYQEAEQSGHFSSKEELLRV
                     TMERIGPGILLAGTSLAISFVPLALTGFKGLAELGVICGLGMLVMTIGTLIILPTLIM
                     VFDRPFRGRWRTVHAPQARPLLPLTRRRSVIILAGAAVALALSAWQAAAVRFDLNMLH
                     LQSPQVESVIWENRLLAGSGLSSIFGEMLAPSLEELRTLSAALKSLPTVERLESIDNI
                     LPRDQEAKRILIEKLRPVLRGVEIAPPPDREVEIAALKEILGRIRFKMLDAREADQKL
                     RGQMAEARGLIDNIRRMLDQGSPEKVQRTLDRFESDLRADLTDKVKLLQANIQSGPLT
                     VAQLPPALRERFVSPDGRFLVKIFPRGDAWEPRFLERFVRELRSVSPEVAGDPVTLSV
                     FTREFRNSTIFAALFSIVFMFLFLLFMLREFKAALLALFPLLAGTVWTFGLMRPLGID
                     LNLANGIFLPLITGAGVEYGIIIVHRWRQERHRGKDAAALPAATGWGVVLAGLTTTFG
                     FGTLMISSHRGIFSLGMLTAIGSLVVLAAAVIILPAILEVMERPRGGSPPRSLAATDA
                     SAPRPHCQSGLQTIALPETTDENPVTKSPLVRGL"
BASE COUNT         2216 a         3722 c         4177 g         2520 t
ORIGIN      
        1 ggggccgcct tcggtattga tgctcagata ctggggcttc atcgtctcga tggcggcggc
       61 gatggtgttt tccggccagt cgtaggcgat ccgtccggcc ccggcgacga attcgacggc
      121 gcttttctcg atggtcagca gatcgatgcg ggccaggcgg ggatcggtct ggacatggtc
      181 cttcaaggtc gcatcgaggg cggacaaacc gcggcgggtg gggaagtatt cgtaataggt
      241 gcttacggtg tcggccagct ctcccaggcg ctgggcctgc tgttcagtga ggagattgtc
      301 gaatacttgc agttgcaccc atccccagac ggccatggtc atggctatgg ccaggacggt
      361 tacgagcatc aggcgggtgc gaatggtcat ggccgggact ttaacataac ttcccggagc
      421 tgcaagaagt ttttgccgcc ccgggaagtg gcggcaaaat gccttcgttc ggaccggacg
      481 attatttggc cgccgggtag ggcggtgggg actttatgcc tttaggagga gagaagacct
      541 tttttttggc gactttgccg acccttttgg cgatatgttg atggtctttc gtgatgtagt
      601 tgacgccgac ttcatcgccc acggcgattt tgtcctgggg tttgggaagg acgaaatcca
      661 tggtttccac ggtgcctttg acattccgtt cgatcttcaa cgccgtcgga gagatctcaa
      721 tgacctttcc cgtggcccgc atgcgcgtct cccggggcgg agccttttta ggtgctggtt
      781 tgatggggac ggttttcacc gtagccgtct tggccggacc aggttctggc tggccgacgg
      841 ccagagaggt cgcaacgact atgcaaagaa gactcaaaga tattgacagc caccgtgtca
      901 ccgacgaact cctgtttttc ccactcggtg acgcaattat tatgccacct ccgggcggaa
      961 gccgaagaaa gaacatccta agcttttgaa aacatgagtc tatttcatga caacacccaa
     1021 atatggtttt tcctgccgtc ggggaggaag gtgaattaat ccccatgaat tgaggaattt
     1081 tgcatcatgt ccttgaccac cggagagttt tcgtgttagc cccccaatgc cggaaaggtt
     1141 gcgattcctt tcctccacgc tttccggaga ggagacgggg tgaaatcccg gcgggatggg
     1201 cggtttgact ggttctgacc gcatccccgt ggtgaccgca tttatcgtcc aagctatctg
     1261 ccgaaggaga cggccgattc gtcttggttc ggaggtttta tcccggcaaa gatcagagga
     1321 ggggacttgg agagaaaatc atttaaatac tatgacatga tcatggctct gttcgttatg
     1381 gtcgtggtca tgagcaacat actttcctcg gccaagatcg tagattgggg catctccctg
     1441 ttcgggatcc cgttggcttt cgatgctggc acgatcctgt ttccagtaag ctatatcttt
     1501 ggcgatatcc tcaccgaggt ttacggctac cggcgatctc ggcgggtgat ctggatgggc
     1561 ttttcctgtc ttatcctttc gagcctgctt ttgtatttcg tcaaaatcat gcccggggag
     1621 ggaatttggg agaaaacggc cgggcaggcg gcttacgatg ccgttctgtc cggcatgacc
     1681 accggaggaa tcgtggcggc cagccttttg gggtactggc tgggagagtt ttccaattcc
     1741 tacgtcctgg cgaagatgaa gatccttacc ggggggcgct ggctctggac ccgcaccgtg
     1801 ggcagcaccc tggtcggtga ggggatcgac accatggtgt tcgtgtccgt ggcggttgtc
     1861 tgcggtgttt tccccgccgc ccttttcatg accttggcgg tgaccaatta tctgttcaag
     1921 gtcggcgtcg aggtgatcat gactccggcg acttacgccg ccgtcgacgc cctcaaaaaa
     1981 gcggagcagg aggatttcta cgatttccgg accaacttca atcccttctc ccgcggctcg
     2041 aatatctatt cctgaatgaa attttctatt tgcttgctca gcctggtctc gatattgttc
     2101 atccccgttc ccagcgccgg ggccgaccga gatttgctga ccaagcaggt actggagcag
     2161 gccacggtca aatatcgaac cgatttcgcc gtcgaggcgc ccctggaaat ctgggacaag
     2221 gttttggccc acccgctgct catggccagg ttgtggaatg tttataattt tcagccggcc
     2281 tacgaggtga aggatcaaaa ggagggggtt ctggtcatcg atccatccgg cattaaggga
     2341 aaactggcca tgaccgaatc caggccggac ggccgcagct tctacggctg ggggtccgtc
     2401 gatcactggg ccattccgtc ctttatcagc gccgaaggaa tctttcgttt tcggtatacc
     2461 ggggacggcc ggcgggtgcg ggggaccttc gaggccttca tgaaggggga caacaaggcc
     2521 accgatttct tgatgaagtt ggtccggggg aaggtgaaaa acctgctgca ccgccggttt
     2581 accaataacc tgaacgacat gaaaaagatt attgccgata ttgtccaaaa tcccgatcag
     2641 atccgtggcc gcctcaccgg cggtgacctc aaggagttca acaacgcctt ttgacacccg
     2701 tcttttgttt tcttcttgat cgctgtagcc gccgttcttg acggtagggg ggaaaaaaga
     2761 caattccggg gtaaatacgg taggacaggg gggtaatcgt tcccgccgtg atggggaaag
     2821 ggggtgattc gccggtgacg gattggaagg aaacagcggg aatggtgatt gtcatcggcg
     2881 ccggtgcggc ggggatgatg gcggcgggcc gggcggccga ggccggggcc cgggtactcc
     2941 tcttggagaa aacggagcgg ccgggcaaga agatcctcgt gagcggcaag acgcgctgca
     3001 acctcaccaa tacccgggag cttcaggaat tcattcccat gtatggagcg gccggctcat
     3061 tcctccgtgc ggcttttcac cgctttttcc gggaagacct cctggtcctg ttgcggcgct
     3121 atggggtgga gacaaaggcc gagcggggcg gacggatctt tcccgtttcc gatcgggcca
     3181 ccgatgtggt tgaggccttt cggcgctatc tggacgagca cggggtggag ctcgtgactc
     3241 ggcagcgggt cctctccctg gtccggcggg agggagcggt gaccggggtg atgacggcgg
     3301 gaggtacctt gcctgcagac gcggtaattg tcgctgccgg tggcgccacc tggccggcaa
     3361 cggggtccac cggtgacggc ttcgagctgg cccgcggcgt cgggcaccga atcgaaaaac
     3421 tccgacccgc cctggtgccg ctcgtcgtcc gggaaaccgt cctggcgaag tccctgcagg
     3481 gggtcaacct gcgcaacata cggcttacgg ccttccgggg gatacccggt gatttcgatc
     3541 cggccttcat tcccgaccgg gacgtcggcc ggggaacggg ggggaggaag gcccggaggc
     3601 ccgtcatcga gagccgctgc ggggagatga tgatgaccca tttcgggatc ggtggtccgg
     3661 tgacgttgct gatgagcctg gccgtcgtta aggccttgga ggaaggaccg gtgagcgtcg
     3721 ccctcgatct caagccggcc ctggattacc ggcagctccg ggcgcgcctc cagcgggacc
     3781 ttgatgccca tgggaagcgg caatgccggg ggattctcgc cggactcctg ccccggaaat
     3841 ttgtgacgcc cttggcggcc caagctggga ttcccccgga caagccagcc aaccaagtta
     3901 cggcggcgga acgggaccgc ctagtgacgg ttctaaaatc actgtgcttt gagatcacca
     3961 gctcccttcc catggcgacg gccattgtca ccgcgggagg ggtttccctg ccggaggtgg
     4021 atccccggac catggcatcg cgcctggcaa cgggtctgta tttctgcggg gaagttctgg
     4081 acgtcgatgc cgacaccggg ggcttcaacc tccaggccgc cttctcaacg ggctgggtgg
     4141 ccggggaatc cgccgccgct tacgccgccc gccggcacgg acatgctccg ccaaatcctg
     4201 aactcttttt gtcttgacag cggggaggcc gttaatctac tcatcaggtc ggaggtgccc
     4261 taacgatgat ggaggcggaa gaaggattga tgcgcctggg catcgttgcc gcgctggcgg
     4321 cggaggcccg catcctctct ccgggggtgg tgccgatgca aaccgtggtt cccttccggg
     4381 gcgccgggct gctgattctg tcgggcatcg gttccgggcg tgccgccggc gccgcccgga
     4441 agctaatcga tcacggcgcg gaggcgcttc tgagctgggg gatcgcggcg gggctggcgg
     4501 cggacctcgc ggccgggacc ctcgtcgtcc cggagacggt ggtcgatgac gccggcgaaa
     4561 cctttgcaac tgatcgtgcc tggcgaaaac gggtaaccgg acgccttggc gcttttctga
     4621 ccgtgagcaa tggcatgctg gtccagggcc gcgccatgct ggtgacagga caggacaaga
     4681 tcaatctggc gaccgccacc ggtgcggtgg ccatggacat ggagagcggc gctgtcgcgg
     4741 cggtagcccg tgaggcgggg atcccttttc ttgctgtgcg ggccatatcc gatccggtgg
     4801 ggatgagcat ccctcccgga gtcctggcgg ccgtcgacag atacgggcgg gtgcgatcct
     4861 tacggatgtt tctccgtctg gcccgtcacc cgggggaagt catgggtata gttgccctgg
     4921 gttggagctt ccgtgccgcc tgccgcacgt tgcgggcggc aggcggggat gagagcgtct
     4981 ttcccccggc aggttgagcc ttttccgcgc acggggcacg aggaattatg aaaatcctga
     5041 tcacgggggc tacgggtttt atcggcgggg cggtgctgcg gcaactggcg gcggcggggc
     5101 atgatgtgcg ggctctggtc cggccgggcg gcgaccggcg gaacttgacg gggctggcgg
     5161 tggaaacatc tgtcggggat ctgcgggatc tcccctcact ggagcgggcg ctgcgggatt
     5221 gccgtattct cttccatgtg gcggcggact accgcctctg ggtcccccgt ccggctgagc
     5281 tttacgagac gaacgtggcc gggactcgga acattgttat ggcggccgcc gcggccggtg
     5341 tggagcggat agtttacacc agcagcgtgg ccgttctggg cctgcaccac gacggccgtg
     5401 ccgccgacga ggacaccccc gtgaccgccg cggatatgat cggtcattac aagcgctcga
     5461 aatttatcgc cgaggccgaa gtccggaagc tcgtcaccga gcggggggct ccggtggtca
     5521 tcgtcaatcc ctccactccg gtaggtccgg gggacgtgaa gccgacgccg accggtcgga
     5581 tcatcgtgga cgccgtttcc gggcggatgc ccgcctatgt ggatacgggg ctcaatttcg
     5641 tccatgtgga cgacgtggcg gcgggacacc tcctggccct ggagcggggc cggatcggcg
     5701 aacgctatat cctgggcggc gtcaacatga ccctgaagaa ggccctggac gagatcgccg
     5761 cgctctcggg atgccggcgg ccccggatcc ggctgcccca tggcctggtc ctggtcctgg
     5821 ccgcgatatc cgaagcctgg tccctcgcca cccgcaccga accccgggtc aatctcacgg
     5881 gcgtgcggtt ggcccggaag aagatgtatt tctccactgc gaaggcccag cgtgaactcg
     5941 gctatacgat ccggccggtc cgggaggcct ttcgggatgc catcgcctgg ttccggcagc
     6001 agggctatct acaatgagct ctcccagggc cgccgcgggc gcggaatcgt tgcgctccgg
     6061 ggcgatagcc cgggaattca agatgcgggg cggttccggt tccgccatgg cggggacggg
     6121 gcgcttcggg tccgtccccg gggaggcatg acgtcattat ggacatctcc attccccagg
     6181 cgctgttgtt gatcccggta atcgccgggt ccgtctatga agtcatccgc ctcgtgatcg
     6241 taaagagatt tttggccggg catatggaca gggacggggt tccgccggga ggctggccgc
     6301 ccgtcaccat cctcaaaccc ctccgcggcc tcgacaaggg attggcggag aatctccgca
     6361 gcgcctgccg gcaggactat ccccggtatc aggtcgtcct tgcggtccag gaccgggacg
     6421 atccggcgtt gccggtgatg gaggagatcc ggcgggaatt tcccgaccgg gtagaactgg
     6481 cggtggagaa cgtcgtcgtg gggttcaacg gcaagataaa caacctggtt ggggcccttc
     6541 gccacgcccg gcatgacatc ctggtgatca gcgacagcga cgtccgcctg cctgaggatt
     6601 atctccggtg catcgtcgcc cctctcgcgg ggccgggggg cgggaaggtc gcctatgcct
     6661 gcaccctgta ccgggccgcc ggcgccggcg cctggtatga aaaactgggc ctcctcacct
     6721 acaacgccgc cttcatcccc gacatcattt ttgcctatgt caccaatgcg gcgccattct
     6781 gcatcggcgc ctcggtcgcg ctgcggcgcc gggatctgga ggcggcgggg ggatttgccg
     6841 ccctggggga ctatctcgca gaggactacg agatggggcg gcgtctcctg gcccggggcg
     6901 gaaggatggt cctgctcccc cgggaggtgg tgatcaccgt agatctgccg agttcggccc
     6961 ggtggtgggc gggccaggtg tcctgggatc tgaaaaccag atccgcccgg cccacgggtt
     7021 tttttgccgc ggtagtgacc cgggccattc cctttgccgc cctcttcgcc gccctgcgtc
     7081 ttgccgacgg tgtggggatg gccgtcctcg ttaccgccat cgtgatccgc ctggggactg
     7141 cggccgccct actgggggta ttacgggacc gggaaggacg gcgagccctg tggctcctcc
     7201 cggtacggga tgtggcgggg tttttttcct gggcggcggc gctggtaagc aggaccgtcg
     7261 tatggcgcgg cacccgatat gtcctggacc ggcatgggcg gctcgccggg gtgcgatgat
     7321 cgcggcaccg ccctgaaggc ggttagacgg ccacttttaa gacaggttga tcatgagggc
     7381 gcttatcatc accgctgacg atttcggttt ttctccggca gtaaacgaag cggtggagga
     7441 ggctcatcgg cggggggtcc tgggaacggc gagcctcatg gtgggggcac cggccactcg
     7501 ggacgccgtg gcgcgggcgg tccggcttcc aggtctgaaa gtgggtctcc atatcgtggt
     7561 ggcgggagcg ccgcccgttc tatctccatc ggaaatcccc gctcttatcg atcctgaggg
     7621 gaaactctcc tcccgcctct tccgggcggg ggttaatttc tatttccgcc cagccgtccg
     7681 ggctcaactc acccgggaga tccgtgccca gtttgagctg tttagggggt acggcctggc
     7741 gctcgatcac gtcaattgcc acaagcacct ccatctccac cccacggtct tcgccctgat
     7801 caccgagatc ggcgccgcct atggtctgaa ggccgtccgt ttgccctatg agcccctgtc
     7861 cggcgccgcc ccggacctcc tccggaaggg cggcgcggga caactggcgg cctcactgtt
     7921 tctcctgccc tggaccgggc tcctgaagag gaagatccgg gcggcggggt tgcgggccaa
     7981 cgactttatc ttcgggatga gggattccgg acggatggag tcggcgcggg tcctgcgctt
     8041 gctgccccgg ctccccgccg gggttacgga gatgtatttc catcccgccg cccggagttg
     8101 tccggagctg accgccgagg ccccgggcgc ccgtcatgaa gcggagtttg ccgccttgac
     8161 ggacccggcg ttccccgccg ccctggccga actgggtatc cggcgggtca gtttttccga
     8221 tcttcctttg aacatccatg catgactgtc tgaatgagac cgggattaat gatccctctt
     8281 cagatggcgg atattcttcc tgctctggga gcgaaaaccg agaaactccc tcccttcctt
     8341 cacgatccgg cgccggtcgt cgctgtttcc cagcgccccg cggaggatcc ggaaaatgat
     8401 gcggggacgg tagtaatagc gtcggtaaaa atcctgcacc gccgccacga tctcatcgcg
     8461 gctcagatcc ggatagctga tgaccgggag ctgatgacct tgggcatcgg ccatttccgc
     8521 cgccacgagg tagccgttgg ccacgaggta gtcgtagaac ctggtgccgg ggaagggatg
     8581 ggatatggag acctggatgg tatcgggatc gagctcgacg gcgaaccgga tggtcctggc
     8641 gatggtctcc cgggtctcgc ccggatggcc gatctggaag tcgccatgga cctggaggcc
     8701 cagccgccgg cagttcttca tcagttcccg ggcctgttcg accgtcgcgc ctttcctgat
     8761 gttcttcagg atgaccgggt ccccggattc gaatcccacc accaggaggc ggcatccggc
     8821 atccttcatc tccctgagga cctcgtagtc cgtattgacc cgggcattgc acgaccaggt
     8881 gaaacccagc ggtttgaagg cccggcagaa ttccatgatc cgctgccggt tccaggtaaa
     8941 ggtgtcgtcg tcgaaaaaga tctcccgggc ctcgggaaaa tgccggaatg cccgtttcaa
     9001 ctccgccacg acattgccta tggagcgggt cctgatccgg tgcccgctca tggtttgcgg
     9061 ccagagacag aaggtgcact gggccggaca gccccgggag gtgtagaagg agacataggg
     9121 gtatttgatg aaggggatgt cgtattgccg gaaatcgaga tcccgcctgt agatgtccac
     9181 ggcgaaggga agggagtcca gatcttccag gggcgggcgg tcggggttgt ggacgatccg
     9241 ttctccttcg cggaagctga tcccggcgat ctccgccgcc ggcttccccc gggcgatttc
     9301 ggccacggcg tggtcgaact ccttccggac aacgaaatcg acggcggtcc cggcccggag
     9361 actctgttcc ggcaggaccg tcacgtgagg cccgaccatg gcgatccgca gcgccgggtt
     9421 ggcggtcttc atgagctccg ccagccggat atcgttggcg aaccccgcgg tgctggtaaa
     9481 aatgacgaca aaggcgaaat cggcggcgat ttgcaccgtc tcctccgggg aaacgccgtg
     9541 ggccggggcg tcgagaaggc ggctctccgg gatcagaccg gccgggtagg ccagccaggt
     9601 cggataccag aaggcggtta tctcccgggt ggcctgatac cgggcgccgg cgccgccgtc
     9661 aaagcccgcg aacgaggggg gatttagtaa cagggttttc atccgttgtc tccggtaaag
     9721 ctattgtttg tagtccgctt tgacagtggg gacggggcgc ggaagcatcc gtcgccgcaa
     9781 gactccgggg tggggaaccc cctctcggtc gctccatgac ctccaggatg gccggcagaa
     9841 taatcaccgc ggcggccagg acgaccaggc tcccgatcgc cgtgagcatt cccaggctga
     9901 agatcccccg atgggacgag atcatcagcg tgccgaagcc gaaggtggtg gtcaagcccg
     9961 ccaggaccac cccccaaccc gtggcggcgg gaagcgccgc cgcatctttc ccgcggtgtc
    10021 gctcctgccg ccaccgatgt acgatgatga tgccgtattc cacccccgcc ccggtgatga
    10081 ggggcagaaa gatgccgttg gccagattga gatcgattcc taaggggcgc atcaggccga
    10141 aggtccagac cgtgcccgcc agcaggggga acagggccag cagggcggcc ttgaattccc
    10201 gcaacatgaa cagcagaaag aggaacatga agacgatgga gaacagggcg gcaaaaatcg
    10261 tgctgtttcg aaactcccgg gtgaaaacgc tcagcgtcac cgggtctccg gcgacctcgg
    10321 gagacacgga gcggagttcc cggacgaaac gttccaggaa ccggggctcc caggcgtcgc
    10381 cccgggggaa gatcttgacg aggaatcttc cgtccggtga tacgaaacgc tcccgcaggg
    10441 ccggcggcaa ctgggcgacc gtcagcggtc ccgattgaat attggcttgc agcagcttga
    10501 ccttgtcggt cagatcggcg cggaggtcgc tttcgaatcg gtccagggtg cgttgaacct
    10561 tctccgggga cccctgatcc aacatccggc ggatattatc gatgagacct cttgcctctg
    10621 ccatctgccc ccggagcttt tggtccgctt cccgcgcgtc taacatcttg aagcggatcc
    10681 ttcccagaat ctccttgaga gcggcgattt ccacttcccg gtccggaggg ggggcgattt
    10741 ccacccccct caggaccgga cgcaacttct cgatcagaat ccgtttcgcc tcctgatccc
    10801 gagggagtat gttgtctatg ctctccaggc gctccaccgt gggcagggat ttcagggcgg
    10861 cgcttaaggt tctgagctcc tccagggagg gggcgagcat ttccccgaag atgctggaca
    10921 gacccgagcc agcaaggagc cggttttccc agattaccga ttccacctgc ggtgactgga
    10981 gatggagcat gttcaaatcg aaccggacgg cggcggcctg ccatgctgac agggccagcg
    11041 ccacggcggc gccggcaagg atgatgacag accgccgtct ggtcagtggg agcagcggcc
    11101 tggcctgtgg ggcgtggacg gtgcgccagc ggccacggaa cggtctgtcg aagaccatga
    11161 tcagggtggg gaggatgatg agggtgccga tagtcatgac cagcattccc aatccgcaga
    11221 tgacgcccag ctccgccagt cccttgaaac ccgtcagggc caggggcaca aaggagatgg
    11281 ccaggctcgt tcccgccagg agtatccccg ggccgatccg ttccatggtt acccggagca
    11341 actcctcttt cgacgagaaa tggcctgact gttccgcctc ctgatagcgg gcgaaccagt
    11401 gggcgccgtt gtcgatcccg agacccagga tgaggggcgc aaaagtgatg gacaggatgt
    11461 tgaggtggcc gaccgcgagg gtggtcaggc cgaagcacca tgtcagggcg acgaccaggg
    11521 tgatcatctc gaacaccgga cggcggatgc ttcgccggaa aatgatgaac aggaccgtca
    11581 ggccggccag ggaaagtacc gtggcgagtt ccatgtcggt catggcggcg ctcatctgat
    11641 cggcgttcag ggcctcggga ccggtgaccc cggccatgat gtccgggaat tccgcccgga
    11701 cctccgccac cgcctcccgg agggccgcca gggagcgctt ggtgccggtg aagtcgttcg
    11761 cgaggcgaac cggggttacg aatgcaagga ggcagcgttt gtccgcggtc cagaaataac
    11821 cttcccgaga ggtgtccccg gcgacccgtt cggagaacag ggcctcccag ggggagttga
    11881 aggggccctg acctgccagg gaccttttaa tttcctccag cacccggatc aggaaagccg
    11941 ggtcgagggg ctcgcctccc tcccgatggt cctccagaaa tcccgtaaac agttcgtcca
    12001 ccacccggct cgacagttcg tggttgagct gggcgaagaa attttccagg gtgggcaggc
    12061 gggagatctc ttgcagaaag cgtttgtttt ccgcaagctt gtcgcggagg cgccgcaggt
    12121 cttgtccgtc gagatacagg agcagccagg gtttcagggc ttccggatcg atgcggtaaa
    12181 aaacctgggc gaagttccgc cggtccgcct cgatacgggg tgcaaggcgc ttcagaaatt
    12241 ccagggccct tgtctcggga ccctcgatga ccacgatgaa ggagtccagt tgttcgaaat
    12301 catgaaactg tctggcgatt tgtatcagtt gctcctgcgg ggagatcaga tcggactggc
    12361 tggcctggat gcccaggttg tttgccgtgt agagcgccgc cagaaccgat atcaccgcga
    12421 cggcgaacat gaccgtccag ggacgtttga tctggaaggc caccagtccg cccagggccg
    12481 attttacgaa ccgcttgatc ttcaccaggt tttcatcccc gtcacatatt ccagctcggc
    12541 ccgggaaagg ttgtaacgga agagggcttc cacatatttt ccctggttgc ggccgtacat
    12601 gtcgatcgcc cggagcatgt tgtccgcgct cccga
//
LOCUS       DAOSMF010000049        12541 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_5842_length_12541_cov_17.729057, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000049 DAOSMF010000000
VERSION     DAOSMF010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 12541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12541
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5842_length_12541_cov_17.729057"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="PLT63_06340"
     CDS             complement(<1..572)
                     /locus_tag="PLT63_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98378.1"
                     /translation="MKIDETNIIGKVEDSLRACLQRVPFLEIDSLKRGLNLGDAPMDI
                     VADVRVAEQRQRLAIEVKSNGQPRVARTAAYRLARVLESDPGLYGVFAAPYISPRTAE
                     ICFQEKIGYLDLAGNCRLAFGQVYIEQQGNPNPFAEKRDLRSLYSPKAARVLRVLLTD
                     PKRPWRTQALAEEADVSLGQVANVKSLLEAR"
     gene            complement(698..3307)
                     /locus_tag="PLT63_06345"
     CDS             complement(698..3307)
                     /locus_tag="PLT63_06345"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOD98379.1"
                     /translation="MAKKNDTQTGDLYKKNIPTMPEGYYSGDKPNPNLKSFVEQHIKE
                     RPFNPVSDDYDVPAFDKPIETTKATAIYNMHTYWSKKPHDAIRQYIRHYTKPGDLVLD
                     PFCGSGGTALASLMEGRKAIAIDRSPAATFITKNYCTPVDVDELQKAFEGLKRKVKPE
                     IDWLYETCCDRCGGKATTAYTVYSQVFQCPRCLEKVPLFDCVEIDGETAKGKPKKISA
                     CPQCHKRGIVEEISTRAKKFGAVPVLVSYLCEGGCKPVRGERRHNDPNHKKREYFGKY
                     DLAKIREIEAKEIPHWIPPNKMMNVADDSVPWGDNWRLGRNFRKIIELHTKRNLWALA
                     LIFSEIEKLSCSTDARDWLRGCVTGIILGVSKMNQYRPGVSFPLNIMVGTYYMPQISK
                     EEFILKHFENKVERVSKGIIEISRMLSSSALLITTDDAARAVAQIPSSSVDHIFTDPP
                     YAGKIQYGELNFVWEAWLNLDTKWHAEEIIVNELRDKSEEDWTYAMRRTMAECIRVMK
                     PGRWLALCYHDTSEGTWALVQDIMAETGFIVDQADRALFIDTGGKTYNQTQSDKVNKR
                     DLVINFRKPKLGEVTATVALTGDEDKTTFNEKVRQIIRDYIGANPGCSKDRIYDEVVS
                     RMVRSGQMEAHDFDELLRQVAEEVKTPIMKNLFEQKDADLFGSHEIGRWYLKEDELTV
                     ADAAETAREDAAADKLGAFMKEILKKRPGDQGVHYSDLFEHYIYAVKDKPRQQMADIL
                     PDYFYKTEQGTWRLPETEEEERAKGEARVKGLGRRIKRYIAQLDQGVVIPDHERPNDA
                     TLSEWIRHCKRSGLYEQGKLLYEKGGLNLDNLTEEAMVNVEEDYQVCVRMLARTGGVA
                     IDSKPKRGKKASS"
     gene            complement(3312..6272)
                     /locus_tag="PLT63_06350"
     CDS             complement(3312..6272)
                     /locus_tag="PLT63_06350"
                     /inference="COORDINATES: protein motif:HMM:NF020249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PglZ domain-containing protein"
                     /protein_id="HOD98380.1"
                     /translation="MLQSWIISKLEPVKECNRVLVRDSLQLLGEGDGTIHRFACENGY
                     TVVKAATNLAFRDLYDQVCADPEIAKVLVIDRAPARRRAASSVTKAPPPFYPDLLALT
                     SPECRIDLDLRQFLKEATGDDNWPAETNDPRYARLIVRNLEAVQRAHKNLRAANPTRF
                     EDHDFRTIVAFAALGVAESAFKKPNAEDYWKIGLLSYEALEELETLTPEITKPIREEL
                     RKAPAPFCHFANHSADLVVRAFYLSVVLSQHSENWNLLLANIDPDLKPLSDIKPDILK
                     DAAIRLVGLDRDQAHHDLTNVEKALSKENLQFLLLDQLKLDQAAGFAAALERERYSTT
                     VASLALLMALNNLVSATPAKPEQQRIMQALFPEDGKKTTRFIDIRPGSTFNQLKEAYQ
                     LADQCNRLRDQLALAMKNLKVTKPEDLSYKHFREIWNDQKINRLEYYLSALQRLAQTG
                     NLLIRHEDELPSLFSNTLAALQQRLKAIKEEVNRQLDDMNARFQELVAREYPNWAKEI
                     GKDGSKADGLPLFASQMPPILTSQFLRRCLKPYWDFQKEKAVLLVFDGMRYDIWDELL
                     RPLFEDRMDIIADLPASSLLPSETHITRKAIFAGTFPESFDTHAAENVLLKEGLAREF
                     GYAGEVEVVSPDTAGTGETVRYRAGNLDVYIFELCDKELHKIAMKTLPDGRQVPSRPL
                     SFVYEQHLKNIVDTEVMAIIRNLVPATKVIVTADHGFGQVGREPLWFSNECLNEPTDC
                     SYLNCWLRVPISDSEIPGKVRNSVVAFTPEQLRMPAKEVRFNKKLGHDVHKEFKAVVF
                     PKTGFSFSRQGSPYHPDAYTHGGISIQELMIPMVVLKVKKHEEGLLILDNITGPQEVV
                     EGEEVEFHLPISKSTHLAAKEKEIRADVDATWSCDPGERPLPHQVLYVSAQGGEAIIR
                     FKPDPSDATVEERKNGLMERTLTVTVSYREGYRISRKMQSRKFAVRLNSEQIVRRIPP
                     SLGNILGLTPKSMR"
     gene            complement(6247..10620)
                     /locus_tag="PLT63_06355"
     CDS             complement(6247..10620)
                     /locus_tag="PLT63_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98381.1"
                     /translation="MSKEDLLTTRKTLDEICTVPDKIISVYSLEKHVWQDSNSEPARK
                     ARKPELQTIEEFQINPVRPFLNDVFRQIAAPYKPERKDSPIGQGYWIQAEFGSGKSHL
                     LCLLAALVLGDKKSWDVVKQKEDKAGRGKRESLYQFWEDGLQTKSTKQKGIFVIVKTL
                     VGAGGGTVGLTDKGQRLSEYILDAANEQIQVELGKNLSLFPTELLADRFINEDLDRYR
                     ADLRKFLRDPRFFEEDEFEDVDSFLKDIQDNKSPEYKRSCGNKLWRFYDEYLKVKPNI
                     AAETEDILKNMVEVILAEGYSGVLLVLDEVSLFMKNRDDNQRTDDEKTLVVLSNRLAK
                     VHNLPIWTVCAAQQAIESKMGVKNIIADDRLKLVELLKDDKDYYDIVLARVREIKDLG
                     AISNYYLYYKRGFTWPNSIGETEFSHFFPFHKPAIEVLRLVTHELTTARSAIHFMHQT
                     LKHHIKNKGNELIRLWELFDEAVRYEEDPSGVHAGLVAIRTKKEVEYKAYETCKRQID
                     GQTKGALKVYREKAVKIVQTLFLYHVAKYKQQGLSPEEISNAVLVERSSDSTPDENIQ
                     HYENISENLKKEMRQIVQNADEDGKPLYRFDPVFSGVDPRAEFKKARDEAEANKVMQR
                     EAWDHLVGLDTWPIRTRQMTIDLANGNSSIFREISPFIGPWEDHANAKAGDQTIDITW
                     QGRNITGLVGMRNIGKLAAENIPLPSINTDETDNDFEVIVGTAPVANDSVVKMLTQRN
                     DPRIIVWTPAELNSEERDKVLDFAAYRKLVSDWQGKESEDAVTVINWVATTLQTELGK
                     IVKVVGSSYARGRIDSSKNNQMEFHVAGELTSIITPLVDKVLAGTYESRDIKFDPPFV
                     FNRDDGVKIINGIVKTGLIPKNAKPNKDISAAQNFGFGLKIMKKSAEKELDLTDNRFI
                     KEMQDFINSKLTEPAQTMKIETIYKNFMGIGGPKDYGLNRRMVQIFLLCLARHGKIRI
                     GIGPKAGLPFPMIDYSNMGDTEFSAKILDAFTEIQKMEKPENWEVLRPYAEKILAQTI
                     PSTHDDAVISGYRTKLQEEFGRQKEATERLSAKAKGLFETLKCPNPYTHELSQVTALF
                     AHDISSGNDIQMALYALKQAFGYKAFDDNQADKAEVDDLANRLKNYRDLQRFLEYETE
                     LRAIQSYCDTTLPELPQLAKTRKIQRKIVEKTANLQPYIDSEVKLKTELVGKIPPGPE
                     ESNTYGLLVKEYSTVYLALHDNVLDRAEGCRNKISEILNGDDLKAFKIMEKITALLPA
                     VSDALEERLQLLADGVFGCPSPSKASVEERLRNNPVHHCNLSFENYAAILDEIDATAA
                     RAYGALNGTINGKLEVFINPAVRARLEQGKKEPIIAKLLDCKSVADVRNLLIKACLKD
                     PAIVDIINRYLKRIVVKTVSLAEFVPSVSTIEKEQVGQLMTEFGTFLEKQFEDMEGDL
                     EALPMLKLEGEKKHAAKLDNQ"
     gene            complement(10649..11224)
                     /locus_tag="PLT63_06360"
     CDS             complement(10649..11224)
                     /locus_tag="PLT63_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98382.1"
                     /translation="MNLGEGFVLFVRATTPRTTEAAKPDKRGNYHYLQSFFQGLPTDQ
                     RQLALSFDEIGAIIQKKLPHIALVDRPWWANTKSSPQGASWTSAGWQVENVFLKARLI
                     VFRKRGANPLASIPRYVRCLLEGAAAMGRPDNHTLMQWIGFCRRVGWFFEGAMLYERS
                     GLSFDALDDVAKAEAEEHYAVCKRELTRYHR"
     gene            complement(11437..11958)
                     /locus_tag="PLT63_06365"
     CDS             complement(11437..11958)
                     /locus_tag="PLT63_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98383.1"
                     /translation="MKDTEIIEALISKLTVPGGRHLYGVLGTYQALKEFSVKLQEAKT
                     TDGRRFRKPLSVNRGILEMIPDDEFKHLAENEAKRPEPTAGHVAKAFETFLRSKLRGK
                     GLVVLAHMEMLFAYHLELNLLRTMAADDDRILLLLPGRRSGGRIIMFPEIEDGNYTLP
                     PSLIAENHLWELK"
     gene            complement(11955..>12541)
                     /locus_tag="PLT63_06370"
     CDS             complement(11955..>12541)
                     /locus_tag="PLT63_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98384.1"
                     /translation="NASYITQRMFPNGKADQALRLFARKYEGLQELRDVCFYRFCKAE
                     PVMIDVISEVIIPAIGIGKIKRRRIHDYLSERFPESKSIADCTKAIVDALSAGAIVHA
                     DRTQLSFAYREISPRSFAFVLHSEFPEPGMHDLAKLEQNRHIVAMLWNPTRILPTLYE
                     LRNLELISKISEIDNVRQFTTKWRLEEAVARITS"
BASE COUNT         2621 a         3387 c         3298 g         3235 t
ORIGIN      
        1 cgagcctcaa gaaggctttt gacgttggca acctgcccca ggctgacatc cgcctcctcc
       61 gccaatgcct gggtcctcca cggcctcttg ggatcagtca ggagcacccg caacacccgc
      121 gccgccttcg gggagtagag ggaacgcaag tcccgctttt ctgcaaaagg attggggttg
      181 ccctgctgtt cgatatagac ctggccaaag gctaggcggc agtttccggc aagatccaaa
      241 tatccgatct tctcctgaaa gcagatctcg gctgtccggg gtgagatata aggggccgca
      301 aacacgccat acaagccggg gtcgctctcc agtacacggg cgagccgata ggctgccgta
      361 cgggcaacgc ggggctgacc attgctcttc acctcgatcg ccaaacgctg ccgctgctct
      421 gcgactcgca catcggcaac gatatccata ggcgcgtcgc cgagattgag ccctcttttc
      481 agggagtcga tctccagaaa aggaacccgc tgcagacagg ctcttaagct gtcctcgacc
      541 tttccaatta tatttgtctc atcaattttc attgaaaagc aacctttcag catttgctga
      601 aaaggtatat tatagtgaaa attgtcagcc gtcaagtagc attttcactg attgtggcat
      661 tttcagcgtt tgctgaaaat agtctttagg gtgaaaatca ggaagatgcc ttctttcctc
      721 gttttggctt gctatcaatg gcgacgccac cggtacgtgc cagcatccgt acgcaaactt
      781 gatagtcctc ttcaacgttg accattgctt cttcggtgag attgtccagg ttgagaccgc
      841 ctttctcgta aagaagcttg ccctgttcgt ataggccgga gcgcttgcag tggcgaatcc
      901 attccgacag tgtcgcatca ttaggccgtt cgtgatcggg aatgaccacg ccctggtcaa
      961 gctgcgcgat gtagcgtttg atacgccgtc ccaatccctt gacccgtgcc tcgcccttgg
     1021 cacgttcctc ctcctctgtt tccggaagac gccaagttcc ctgttcggtc ttgtagaaat
     1081 aatccggcag gatatcggcc atttgctgcc taggcttatc tttcactgcg taaatgtaat
     1141 gttcgaaaag gtcactgtag tggacgcctt ggtcgccggg acgcttcttt agaatttctt
     1201 tcatgaatgc accgagctta tccgctgctg catcctccct tgccgtttca gcagcgtcgg
     1261 caacggtcag ttcatcctct ttcagatacc atctgccgat ctcatgactg ccgaacagat
     1321 ccgcatcttt ctgctcgaac aggtttttca tgatcggcgt tttgacttcc tcggccactt
     1381 gccgtagaag ctcatcaaag tcatgcgcct ccatttggcc gctgcgtacc atcctgctga
     1441 caacctcgtc gtatatccgg tctttggaac agccgggatt cgcgccgatg taatcgcgaa
     1501 taatctggcg gaccttctca ttgaaggtcg ttttatcttc atcgccggtg agtgcgacgg
     1561 ttgccgtcac ctcgccgagc tttggcttgc gaaaattaat gacgaggtcg cgcttgttga
     1621 ccttgtcgga ttgagtctga ttataggtct ttccacccgt atcgataaat aatgcccgat
     1681 ctgcttgatc cacaatgaat cctgtctctg ccatgatatc ttgtaccaaa gcccaggttc
     1741 cctccgaagt atcgtgatag cagagcgcta accagcggcc aggcttcata acccggatac
     1801 actctgccat agttcttcgc atagcatagg tccaatcttc ttcggactta tcgcgtaact
     1861 catttacgat aatctcctct gcatgccatt tagtgtcaag gttcagccat gcctcccata
     1921 caaaattcaa ttcaccatat tgaattttcc ccgcataggg aggatctgta aaaatgtggt
     1981 ccaccgagga acttggaatt tgcgcaactg cgcgtgccgc gtcatcagtt gtaatcaaaa
     2041 gagcagatga tgaaagcatt cttgaaattt ctattattcc tttgctgaca cgttcaacct
     2101 tattctcaaa gtgctttagt ataaattctt ccttacttat ttgaggcatg taataagtcc
     2161 ccaccattat gtttaggggg aacgagacac caggacggta ttgattcatc ttgctgacgc
     2221 caagtataat tcccgtaaca catccacgaa gccagtctct ggcatccgtg ctacagctta
     2281 atttttctat ctctgaaaaa atcaatgcta gcgcccacag gttacgtttt gtatgcaact
     2341 caatgatttt gcggaaatta cgcccaagcc tccagttgtc accccacgga acagaatcat
     2401 ccgcgacatt catcattttg tttggcggaa tccagtgagg aatctctttg gcctcaatct
     2461 cgcgaatttt agcaaggtca tacttcccga agtactcacg ctttttatgg ttcggatcgt
     2521 tatggcgccg ctcgccgcgc accggtttac agccaccttc gcagagatag ctcaccagta
     2581 ccggtaccgc gccgaacttc ttggcccgtg tgctgatctc ctccacgatg cccctcttgt
     2641 gacattgtgg gcacgcacta atcttttttg gttttccctt ggccgtttct ccatcgatct
     2701 ctacacaatc aaatagtggc accttctcca aacaccgcgg acactgaaat acctgggaat
     2761 ataccgtgta ggccgtagtc gctttaccgc cgcaacgatc gcagcaagtc tcgtagagcc
     2821 aatcaatctc tggctttacc ttgcgcttga gcccctcgaa agctttctga agttcgtcta
     2881 catcgaccgg agtgcagtag ttcttggtga tgaacgtcgc cgcgggagac cggtcgatag
     2941 cgatggcctt tcgtccctcc atgagcgaag ccagggctgt gccgcctgac ccgcagaagg
     3001 gatcaagaac gagatcgcca ggcttggtat aatgccggat atactggcga atggcatcat
     3061 gaggcttctt cgaccaatag gtgtgcatgt tgtaaatggc cgtggccttg gtggtctcga
     3121 tgggtttgtc gaatgcaggc acatcgtaat catccgacac cggattgaaa ggcctctcct
     3181 tgatgtgctg ttcaacaaaa gatttcaggt tcggattcgg cttgtcgccg gagtagtacc
     3241 cttccggcat cgtggggata ttctttttat agaggtctcc cgtttgtgta tcgtttttct
     3301 tggccatcgc tttacctcat gctcttggga gtcaatccta aaatattgcc caacgagggt
     3361 ggaatccgcc ggacgatctg ctccgaattc aagcgcacag cgaacttccg ggattgcatc
     3421 ttccgactga tccggtaacc ctcacgatag cttaccgtga cggtaagcgt acgctccata
     3481 aggccatttt tgcgctcttc gactgtcgca tcactgggat cgggtttgaa ccggatgatc
     3541 gcttcccctc cctgtgctga gacataaagc acctggtggg gcagtggtct ttcgccagga
     3601 tcgcaactcc aagtcgcatc tacgtcggcg cggatttcct tttcctttgc cgcaaggtgc
     3661 gtcgacttgc tgatgggaag atggaattca acctcctcgc cttcgacgac ctcctgcggt
     3721 ccggtaatgt tatcaaggat caaaagccct tcttcgtgtt ttttcacctt gaggacgacc
     3781 attggaatca tcagctcctg gatggatatg cctccgtggg tataggcgtc aggatggtaa
     3841 ggcgatccct gccggctgaa ggaaaaaccg gttttgggga aaacaacggc tttgaattcc
     3901 ttgtgaacat catgcccaag cttcttattg aaacggacct ccttggctgg catccgcaac
     3961 tgttccggag taaacgccac gacgctgttt cgcaccttgc cggggatttc ggaatccgat
     4021 atcggaactc taagccagca attcagataa gaacagtctg taggttcgtt aagacattca
     4081 ttagaaaacc agaggggttc acggcctacc tggccgaagc cgtgatcggc agtcacgatg
     4141 accttggtag cgggcacgag attgcggatg atggccatga cctccgtatc gacgatattt
     4201 ttcaggtgct gctcatagac aaacgacaga ggccgggaag ggacctggcg cccgtcggga
     4261 agggttttca tggcgatctt gtgcagttcc ttgtcgcata actcgaagat gtagacatcc
     4321 agatttcctg cccggtatcg gaccgtttcg ccggttccgg ccgtatccgg tgagacgacc
     4381 tcaacctctc ccgcatatcc gaactcccga gcaaggcctt ctttcagcag gacgttctcg
     4441 gctgcatgcg tgtcaaagga ttcgggaaat gttcctgcga atatcgcctt gcgggtgatg
     4501 tgggtttccg agggaagaag ggatgaagcg ggcaaatcgg cgatgatgtc catccgatcc
     4561 tcaaagaggg gccgcagcag ttcgtcccaa atgtcgtacc gcatcccgtc aaaaaccagc
     4621 agtaccgctt tttctttctg aaaatcccag tatggcttga ggcagcgccg gagaaactga
     4681 ctggtcagga taggaggcat ctgggaggca aacagcggca ggccgtcggc cttgctgcca
     4741 tcttttccga tttcttttgc ccagttggga tactcacgcg ccaccagctc ctgaaaacgc
     4801 gcattcatgt cgtccaactg ccggttgacc tcctccttga tagccttcaa tcgttgttgg
     4861 agcgcagcaa gcgtgttact gaaaagcgac ggcagctcgt cttcgtggcg gatgagcaaa
     4921 ttgcccgtct gggcgagacg ttggagggcc gaaaggtagt attcaagccg attgattttc
     4981 tgatcattcc aaatttcacg aaaatgcttg tacgagagat cctccggttt cgtaaccttc
     5041 aggttcttca tcgccagggc aagctggtcc cggagccggt tgcattggtc ggcgagctga
     5101 taggcttctt ttagctggtt gaatgtggag ccggggcgga tgtcgatgaa ccgggtcgtt
     5161 tttttaccgt cttcaggaaa gagggcctgc atgatccttt gctgttccgg tttggccggc
     5221 gtcgcagaga caagattgtt gagagccatc agcagagcga gggaggcaac ggtcgtcgaa
     5281 tatctctccc tctccagtgc ggcggcgaaa cctgccgctt ggtccagctt tagctgatcg
     5341 aggaggagaa actgcaggtt ctccttgctc agagccttct ccacgttggt cagatcgtgg
     5401 tgggcctgat cgcgatcaag accgacaaga cggatggcgg cgtctttgag gatatccggc
     5461 ttgatgtcgg acaacggctt gagatcgggg tcgatgttgg ccagaagcag attccagttc
     5521 tcgctgtgct gggacaggac cacagaaagg tagaaggccc gcacaaccag atcggcgctg
     5581 tggttggcaa agtggcagaa tggcgcaggc gccttgcgca actcttctcg gatcggtttt
     5641 gtgatttccg gcgtgagtgt ttccaattct tccagggctt cgtaactgag cagcccaatc
     5701 ttccagtagt cttcggcgtt gggtttcttg aaggcggact cggcaacgcc gagcgcggcg
     5761 aaggcgacaa tggtcctgaa gtcgtggtct tcgaaacggg tgggattggc tgcccgcaga
     5821 ttcttatggg cgcgttgaac cgcttcaagg ttccgcacaa tgagccgggc ataacgagga
     5881 tcgttggtct cggccggcca gttgtcatcg cctgtcgcct ccttgagaaa ctggcgcaga
     5941 tcaagatcga ttcgacattc gggagaggta agggcaagta ggtcgggata gaacggcggc
     6001 ggcgccttgg taacggatga tgcggcacgt cgtcgcgccg gcgcacgatc gatgacgagg
     6061 accttggcga tctccggatc ggcgcacacc tggtcgtata ggtcgcgaaa ggccagattg
     6121 gttgcggctt ttactactgt atagccgttc tcgcaggcaa acctgtgtat cgtgccgtcg
     6181 ccctcgccaa gcagttgcag cgaatcccgg accaggacgc gattgcactc cttgacgggc
     6241 tcaagcttac tgattatcca gctttgcagc atgcttcttt tccccttcca gtttcagcat
     6301 cggcagggcc tccaagtctc cttccatatc ctcgaactgt ttctccagga aggtaccgaa
     6361 ttccgtcata agctggccga cctgctcttt ttcaatcgtg gaaacactag ggacaaattc
     6421 cgccagagag acggtcttca ccacaatccg tttcagatag cggttgatga tatccacgat
     6481 ggcgggatct ttcaggcatg ctttgatgag caggttgcgc acatcagcga ccgatttgca
     6541 gtccaggagt ttcgcaatga tcggttcttt tttcccctgc tccaggcgtg cacggaccgc
     6601 cggattgatg aacacttcca gtttgccgtt gatagttcca ttgagtgcgc cgtaagccct
     6661 tgcggcggtt gcgtcgatct catcaaggat ggcggcataa ttctcgaagg agagattgca
     6721 gtgatggaca ggattgttcc gcaggcgttc ctccaccgat gcctttgacg gcgagggaca
     6781 accgaacacg ccatctgcga ggagctgcag cctttcctcc agtgcgtccg acaccgccgg
     6841 cagcaaggcc gtgatcttct ccatgatctt gaaagccttc agatcatcgc cgttgagaat
     6901 ttcactgatt ttgttccgac agccttccgc ccggtcgagc acattatcgt gcagggccag
     6961 atataccgtg ctgtactcct tcaccaggag tccgtacgtg ttggactcct ccggtccagg
     7021 cgggatcttg ccgaccagct cggtcttcag cttgacctcg gaatcgatgt agggctgaag
     7081 gtttgctgtt ttctccacaa tctttcgctg gattttgcgc gtcttggcaa gctggggaag
     7141 ctccggcagg gttgtgtcgc agtaggactg aatggctcgc aattcggtct catactccaa
     7201 gaaccgctgc aggtcgcgat agttcttcag acggtttgcc agatcatcga cctccgcctt
     7261 gtcagcctgg ttgtcgtcga acgccttgta gccaaacgcc tgcttgaggg catacagcgc
     7321 catctgaata tcgttgccgc tgctaatgtc atgggcaaac aaggccgtga cctgggaaag
     7381 ttcatgggtg taaggatttg ggcacttcag cgtttcaaag agcccttttg ccttagcgga
     7441 cagacgctca gtcgcctcct tttgcctgcc gaattcttct tgtagcttgg tccgatagcc
     7501 ggagatcacc gcatcgtcat gggtggaggg aatcgtttgt gcaagtatct tctcggcata
     7561 gggccgcagt acttcccagt tctcaggctt ctccatcttc tggatttccg tgaaggcatc
     7621 cagaatcttt gccgagaatt ccgtatcgcc catattagag tagtcgatca tcgggaacgg
     7681 cagcccggcc ttcggaccaa tgccgattcg gatcttgccg tgtctggcca agcagagcag
     7741 gaatatctgg accatgcgcc gattcaggcc gtagtccttc ggtccaccga tccccatgaa
     7801 gtttttataa atggtttcga tcttcatggt ctgggcgggt tccgtcagtt ttgagttgat
     7861 aaaatcctgc atctccttga taaagcggtt gtccgttaaa tccagttcct tctccgcgct
     7921 ctttttcata atcttcaggc cgaagccgaa gttctgagcg gcgctgatat ccttgttggg
     7981 cttggcgttc ttgggtatga gccctgtctt gacgatccca ttgatgatct tcacgccatc
     8041 gtcccgattg aacacgaatg gcggatcaaa tttgatgtcg cgcgattcat aggtgccggc
     8101 aaggacctta tcgaccaatg gggtgatgat gctggtcaat tccccggcca catgaaactc
     8161 catttggttg ttcttcgacg aatcaatccg gccgcgggca tagctgctgc cgaccacttt
     8221 gacaatcttt cccaattcag tctgtagggt ggtggcgacc cagttgatga cggttacggc
     8281 gtcctcgctt tccttgccct gccaatcgga cacgagcttt cgataggccg cgaagtcgag
     8341 cactttgtcc cgctcctcgc tattgagttc ggcgggcgtc cagacaatga tgcgcggatc
     8401 gttgcgctgc gtgagcatct ttaccacgga atcgttggcc accggcgcag tgcccacaat
     8461 cacttcgaag tcgttatctg tttcatcggt attgatggag ggcaagggga tattctcggc
     8521 ggccagtttc ccgatatttc tcatccccac gagccctgtg atgttccttc cctgccaggt
     8581 gatgtcgatg gtctggtcgc cagccttggc gttggcgtga tcctcccatg gtccgatgaa
     8641 cggcgaaatc tccctgaaga tggaactgtt gccattggcc aggtcaatgg tcatctgacg
     8701 ggtgcggatg ggccaggtat cgagaccgac cagatgatcc cacgcctccc tttgcatgac
     8761 cttgtttgcc tccgcctcat cccgcgcctt cttgaattca gcacgaggat ctacgccgga
     8821 aaaaacgggg tcgaaccggt aaagcggttt gccatcctcg tcggcattct gaacgatctg
     8881 ccgcatctct tttttcagat tttcggagat attttcatag tgctggatgt tttcatcggg
     8941 tgtggaatca gaggaacgct cgacgagcac ggcattagaa atctcttccg gcgacaggcc
     9001 ctgctgtttg tatttggcga catggtagag gaacagggtc tgaacgatct ttacggcctt
     9061 ttcccgataa accttcaagg cgcctttggt ctgaccgtct atctgacgct tgcaggtttc
     9121 ataggccttg tactcgacct ccttcttggt cctgatcgcc acaaggcctg catggacgcc
     9181 gcttgggtcc tcttcatacc gcacggcttc atcgaagagc tcccagaggc ggatcagctc
     9241 attccccttg tttttgatat ggtgtttcag ggtctggtgc atgaagtgaa tggccgaccg
     9301 ggccgtggtc aattcgtggg tcacaagccg gaggacctct atggccggtt tgtgaaaggg
     9361 gaagaaatgg ctgaactccg tttccccgat gctgttgggc caggtgaagc cgcgcttgta
     9421 gtagagatag taattgctga ttgctccgag atccttgatc tcccgcaccc tggccaggac
     9481 gatgtcgtag tagtccttgt catccttcag gagttccacc aacttcagac ggtcatcggc
     9541 gatgatattc ttcaccccca ttttgctctc gattgcctgc tgggccgcgc agaccgtcca
     9601 gatggggagg ttgtggactt tggccaaacg attcgaaagg acgacgaggg ttttttcgtc
     9661 atcggtgcgc tggttatcgt cgcggttctt cataaacagc gagacctcat cgaggaccag
     9721 caataccccg ctgtatcctt ccgcaaggat aacctcgacc atgttcttga ggatatcctc
     9781 ggtctcggct gctatattcg gcttcacctt cagatattcg tcatagaagc gccacaactt
     9841 gttgccgcag ctccgcttgt actcggggga tttgttgtcc tggatgtctt tgagaaaact
     9901 atcgacatct tcaaattcgt cttcctcgaa gaagcgaggg tcgcggagaa attttcgaag
     9961 atcggcgcga tagcggtcga gatcttcgtt gatgaagcga tcggcaagca gctcggtggg
    10021 aaagagagac aagtttttcc ccagctcgac ctggatttgc tcgtttgccg catcgaggat
    10081 gtactcagac agccgttgcc ccttgtccgt cagaccgaca gtgccgcccc cggcaccgac
    10141 aagggtcttg acaatgacga atatcccctt ctgcttggtg cttttcgttt gaaggccgtc
    10201 ttcccagaac tggtagaggg actccctttt gccccgacct gctttgtcct ccttctgctt
    10261 gacgacatcc cacgatttct tgtctccgag gacaagtgcc gcgagcaagc agagcaaatg
    10321 agacttgccc gaaccgaatt cggcttgaat ccagtatcct tggccgatgg gactgtcttt
    10381 gcgttcaggc ttgtagggcg cggcgatctg acgaaagaca tcattgagaa agggccggac
    10441 cggattgatc tgaaattctt ctatggtctg caattccggc ttgcgcgcct tgcgtgcagg
    10501 ctcggaattt gagtcttgcc agacatgctt ctccagtgag taaacagaga taatcttgtc
    10561 cgggacggtg caaatctcat ccagcgtttt tcgggtggtc aagagatctt ctttcgacat
    10621 taaaacctcc tcatgtcggt agataaagct atcgatggta acgtgtcagt tcacgtttgc
    10681 agacggcata atgctcttcc gcctccgctt tcgcgacgtc atcgagggcg tcgaatgaca
    10741 atccgcttct ttcataaagc atggcgcctt caaagaacca gccaaccctt ctgcagaatc
    10801 cgatccattg cataagggta tgattgtcag gccggcccat ggctgccgcg ccttccagga
    10861 gacaccggac atagcgggga atactggcta atggatttgc cccgcgcttg cggaaaacga
    10921 taagccgagc cttgagaaac acattttcca cttgccatcc cgcagaggtc caggaagccc
    10981 cctgtgggct tgactttgta ttggcccacc aaggtcgatc gaccaaggct atatgcggca
    11041 gcttcttttg aatgatggcc ccaatttcgt cgaatgataa agccagttgc cgctgatccg
    11101 tcggcaaacc ctgaaaaaat gattggagat agtggtaatt gccgcgtttg tcgggctttg
    11161 cagcctctgt cgttctgggt gtggttgcgc ggacaaacaa aacgaagcct tcgcctaggt
    11221 tcactttatc gactttccat cccgcattaa gccatgattt cgcgtggttg ctactcaggg
    11281 tgtttgccca ccagcatcgg tcaacccgcg cggttcgggg cagtacggac cccatgattg
    11341 attccatgtt gtcaaacgtc aacctcaagt cccgttcgtc gggacccttt acggccagat
    11401 aatctctaaa agggcggtac ttcgacattg tggcggttac ttcaactccc atagatggtt
    11461 ttcggcgatg aggctgggcg gcagcgtgta attgccgtcc tcgatctccg gaaacatgat
    11521 aatacgtccg ccgcttcttc ttccgggaag caggagaaga atcctgtcgt catcggcggc
    11581 catcgtgcgc agtaggttga gctccagatg gtaggcaaaa agcatttcca tgtgggcaag
    11641 gaccacgagc ccctttccgc gaagttttga gcggagaaac gtctcgaagg ccttggcaac
    11701 atgacccgct gtcggctcgg gtcgcttcgc ctcgttttca gccaggtgct tgaattcgtc
    11761 atctggaatc atctccagta tgccccgatt gacggataag ggtttcctga atcttcttcc
    11821 gtcggtcgtt ttcgcttcct gcaatttcac cgaaaattcc ttgagggctt gatacgtgcc
    11881 gagcacacca tagagatgac gccctcccgg aaccgtcagc ttgctgatga gagcttcgat
    11941 aatttccgta tctttcatga agtaatcctt gccaccgcct cttccaggcg ccacttggtt
    12001 gtgaactgtc ggacattgtc aatctctgag atcttgctga tcagttctaa atttctcagc
    12061 tcgtaaagcg tgggcaggat gcgcgtcggg ttccacagca tcgccacgat atggcggttc
    12121 tgctcaagtt ttgccaaatc gtgcatcccc ggctcaggga attcgctgtg aagcacaaaa
    12181 gcaaaagatc ggggcgatat ctctcgatag gcgaaagaaa gctgtgtgcg gtccgcgtgg
    12241 acaatggcgc cagcggacaa ggcatcaacg atcgcctttg tgcaatctgc aatgctcttc
    12301 gactccggga aacgttcgga gagataatca tgaatgcggc ggcgcttgat tttcccaatc
    12361 cctatggcgg gaataataac ttctgatatc acgtcaatca tcacaggttc ggccttgcag
    12421 aaacggtaga aacaaacatc gcgcagctcc tgcaatcctt catatttacg ggcgaaaagt
    12481 cttagcgctt gatccgcttt gccattcggg aacatccgtt gcgtgatgta gctggcgttg
    12541 c
//
LOCUS       DAOSMF010000050        12537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_5845_length_12537_cov_24.253325, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000050 DAOSMF010000000
VERSION     DAOSMF010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 12537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12537
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5845_length_12537_cov_24.253325"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="PLT63_06375"
     CDS             complement(<1..688)
                     /locus_tag="PLT63_06375"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOD98385.1"
                     /translation="MINAKHLLLMTAIILCLAGMLPRGALCREQISPKVMNLEQIVAL
                     AVAQNPEIEEVQSDVLAARSDLQQVEAAYYPQIEAIAVAGPMRDAKEPLVVNGRITDP
                     SPGLSFSSIGIFGKFDITATQPLYTFGKISNRKEAAARGVKAKEHAVADKKGQIVLRV
                     RQLYYGLVLASSGLNYADESMDFFASAAQRIRKLLELESTTVKESDLYQVEAYRSEAV
                     RFRSEAEKGMK"
     gene            complement(727..1716)
                     /locus_tag="PLT63_06380"
     CDS             complement(727..1716)
                     /locus_tag="PLT63_06380"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HOD98386.1"
                     /translation="MKKFHLMALVLGVSLLVFLIWKIGPAALWHDFYLLGWGLVPFIL
                     MEGVVKIFHTVAWRQCLTHPHSSLPFSRLYNIDLAGGAINYFTPTATVGGEVVKGMLL
                     AAEHQGPGAATGVIINKLSYALSQFATALIGSLIVIRQISLPAYGYWALLAGNLFLGA
                     GLAGFLIVQYQGKLGAALRWLATRLRLESLKRTVAYITEIDQDLRQFYRDNPRGLWAS
                     IGWHLTGFVVSMVKTWYFLSLFTGDNFFTAAAVWLLATWLDLLVFFIPLEIGLQESIR
                     VMVFSVLGFTTALGLTYGIALRLEQIFWGVAGLGVYWAIMPDSRTKAVPTGEG"
     gene            complement(1736..2377)
                     /locus_tag="PLT63_06385"
     CDS             complement(1736..2377)
                     /locus_tag="PLT63_06385"
                     /inference="COORDINATES: protein motif:HMM:NF017319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOD98387.1"
                     /translation="MDMNHNMRWLSTIVCLLLLAAVLIPAARGSAQDPGEATAGESVR
                     DMLDQVLAIQTDPRLQDRQHRDERRRAIKAVIARNFHFDGMARQALGKYGEGLDASQR
                     ASFLALFQDLFQDSYTKLVLDFLKRERILYDREEVAPDRSLIKTRIVRMSEEIPVEYS
                     LAPVDGKWLVQDVKIDGISITENYRRSFARVIQRDSFRVLLDKMRLQRQAMEK"
     gene            complement(2479..3621)
                     /locus_tag="PLT63_06390"
     CDS             complement(2479..3621)
                     /locus_tag="PLT63_06390"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HOD98388.1"
                     /translation="MSFSGHDETNAPGAIAREQGELGNMHAADICRPDLDFEFEALKK
                     VSRSFALTIPELPADLGRVVTNAYLICRIIDTIEDDEALPLDVKARFFKEFVQVLNAE
                     APAVPFAENLTARLSDGVPEAERELVKNTPVIIGGNLSLTPPQRQVIRKCGTVMAQGM
                     LEFQRLKKPGGLEDLEALNRYCYFVAGVVGEMLTELFCDHAPAIAGRRQELLALAASF
                     GQGLQMTNIIKDLWEDQRQGTCWLPRSVFAAVGCDLNRLTPGDGGEAFARGIAEMIGI
                     TRGHLNNALSYTLLIPPEEVGIRRFCLWAIGMAVFTLRNINKNKLFCSGGEVKISRQT
                     LRSIIMVCNMTQRNDFLLAGLFAWITHDLPAARTNAAGSRGGPPLP"
     gene            complement(3672..4679)
                     /gene="hpnH"
                     /locus_tag="PLT63_06395"
     CDS             complement(3672..4679)
                     /gene="hpnH"
                     /locus_tag="PLT63_06395"
                     /inference="COORDINATES: protein motif:HMM:TIGR03470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HOD98389.1"
                     /translation="MGIPLIQKLRISRYIYRQKATGRSRFPMVLMLEPLYRCNLACAG
                     CGKICWPEEVLDRRLSVAECLSAAEECGAPVVSIAGGESLIHADLPQIVAGLTVRKKF
                     VYLCTNGILLKKKLAAYRPSPYLTFSIHLDGFRELHDRIVGKDGVFDTAVRAMQLLKN
                     RGFRFTVNCTIFNGVSAVQAGEFFDFIMHLGAEGITVSPGYDYPGAPQKGIFLKRPES
                     KQIFREVFRLGRRRKWRFNHSRFFIDFLSGNRIYDCAPWSTVTRNIFGWQRPCYLFAD
                     GYAASFRALMEETAWEHYGPGKNPQCEHCMLHSGFEGTAVMDMMTHPVQALAGYLKPL
                     G"
     gene            complement(4686..5483)
                     /locus_tag="PLT63_06400"
     CDS             complement(4686..5483)
                     /locus_tag="PLT63_06400"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOD98390.1"
                     /translation="MTIEPRTFTDIHHALIIAAGRGRRFHADTEHRPKPLLEIDGQPL
                     IMHVIGRAARAGIDSFTVITGYRGEDIEAYLTRETPAGICIRCIRNTEWERPNGISVL
                     AARGAVPDAFALMMADHLFDPRLLGKLFSTPLIEGHCRLAVDFHPPGVPDLEDATKVK
                     ANDGQVLDIGKEIPYYNGIDTGVFLCTEGIFPALETAVSRERESLSEGVRELARRGRM
                     EAMDVTGLFWLDIDDETGLFTAKAALRGISPLHAQLARGQTDDSMGR"
     gene            complement(5473..6819)
                     /locus_tag="PLT63_06405"
     CDS             complement(5473..6819)
                     /locus_tag="PLT63_06405"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOD98391.1"
                     /translation="MRKRRLTTLAMGKKLLIIQPSHYRSRSERTVVKIDKRKVVPLTL
                     PYLAALTPADWEVKLLDEQLDGIDFAAPADLVAITVWTINSLRAYDIADRFRQRGLPV
                     IMGGPHMSFHGDEASEHCDAVGIGEGENIWRQMLADAENGSLRKLYRSDRLCDLQGLP
                     GPRYGLLNMDKYGYFKTFSVQSSRGCPFKCEFCSERLYLGSQYRCRPIAEVVEEIKRC
                     GSRYILFADSNFAGRVENAMKLMEAIIPLKLRWSALWSLRLCADKEFLNLAQRSGLLH
                     VNIGIESISADTLVEMNKKINMVNQEEILRDLRKRGISYSLNFIFGSDADREDIFPAT
                     LSFLMRNKVPAAYLNILTPHKGSALYDRLEAENRIIDSDGIGRWPGMSCHIKHPHFSP
                     ADLLRYVEELHNKFYSYRSMLARLPMPFTRAAVASWVVNFTERNSFQIGAENFSDY"
     gene            complement(6816..8069)
                     /locus_tag="PLT63_06410"
     CDS             complement(6816..8069)
                     /locus_tag="PLT63_06410"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOD98392.1"
                     /translation="MTDQEQKEGKISIILTPDTEGIKKVFGVPAARRLALLCTQIGFG
                     EIHIVGGTDHFKELLSDLAAPDGFHPVGDPGELDDAANAILASHPRPEGGKILVMRAD
                     LVVDKANLQNLAQMGEEPQIPCLESDGSGARIFLSSYRNLAAVMKQAWTLGGNSGDTC
                     SEFSILSGKNGLPCRLKGAGNGKNAERKLVDAIRAQTKATDGFMSRHVSRHISLMMST
                     RLALTRVTPNQVTLVGAGIGLLGAFFIAQGGYWHQLAGCLLFLFCIIIDGIDGEIARM
                     KLQESEFGHKLDIVTDNIVHVAIFSGLAAGCYRQTGDHLYIQLLAVLLGGFALCGAVV
                     YLRLLKPEVESFRSPALGRLLTMMSNRDFAYLMVLLAAVGRLNWFLIGAAFGSYIFAA
                     ALWMATGAEKNVSAAARTDSEMPTR"
     gene            8401..9504
                     /locus_tag="PLT63_06415"
     CDS             8401..9504
                     /locus_tag="PLT63_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HOD98393.1"
                     /translation="MNKIRIALIGIGNCASALIQGIEYYKDKSPQELIGLMHWQIGQY
                     RPGDIEVVAAFDIDERKVGRDVSEAVFQLPNCTTVFNPHIPAAGVIVHMGRILDGMAD
                     HMNDHEDKYTFVPARRKEATKKDIVAELKRTGAEILINYLPVGSEKASRYYAECALAA
                     GVAFINCIPVFIASDTRWARRFTERNLPIIGDDVKSQMGATVIHRVMTHLFQARGVKL
                     EKTYQLNTGGNTDFLNMLDRSRLQSKKESKTEAVQSQTKKRLESENIHIGPSDWVPWQ
                     KDNKVCFLRMEGKIFGNIPMNLEMRLSVEDSPNSGGVIIDAIRCCKLALDRGKGGVLF
                     SPSAYFMKHPPRQFFDEDAYQMLEDFIREKRDS"
     gene            complement(9520..11028)
                     /locus_tag="PLT63_06420"
     CDS             complement(9520..11028)
                     /locus_tag="PLT63_06420"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOD98394.1"
                     /translation="MSMESFDLIVIGGGPGGHAVAEEAALQGARVCIIERNGWGGTCT
                     HRGCVPTKALLVCSRHYVDLKKIKRFGISLGEAGFDYAVIRRHRDQMTRISALGVEKT
                     LQAAGVTMRNGYGRILSSGKVQWTSATGESETLAASHLVIAWGSEPSLPPFLLPKQDI
                     DGRESPASRRGTSSRSEALISESGALSSVAAASANDGRRSPDDVPGRIPGRVLTSDAF
                     LQLAALPQSVVIIGGSVIGVEFATFLAEVGTKVEIVEILPSILPYEEEEAAEFLAREL
                     QKMGIKIHTALSVGSLEEKGGGVIVGGDRDGNRLELEAEYALVCTGRRPRIDEESLRE
                     MDIRYDRRGIAVNEHQETNIAGIYAIGDVTGGIMLAHRAQQQGRALASRLFGDGTVVY
                     REEMVPSVTYTHPPIARIGLTERQARENGWNVEIRRTDYGVNIMARTELAGPGFAKFI
                     FHAGRLVGATIAGEGAPDLIAALTLAMTVGADLKTLRGWILPHPTLSEVLRL"
     gene            complement(11301..12188)
                     /gene="lipA"
                     /locus_tag="PLT63_06425"
     CDS             complement(11301..12188)
                     /gene="lipA"
                     /locus_tag="PLT63_06425"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOD98395.1"
                     /translation="MKEARSLPEAEGGEYLRKPPWLKVRLPGTKGFSRVRDTLTRHGL
                     HSICQEARCPNMAECFQDGTATFLLMGDVCTRNCRYCNVKHGVPGPVDPGEIPRLVAA
                     VEYLNLKYAVLTSVTRDDLYDGGAAVFANAVTSLRAALPAIRIEVLIPDFQGHKEDLE
                     KVIAAGPDMINHNIEVVPSLFTDLRPQGNYAQSLEILRHLSRGKTASCSKSGFMIGFG
                     ETGEDIRRLLADLAAVRCQAVTVGQYLQPTRCHAPVRKYYHPDEFAAIREEALALGIP
                     HVEAGPMVRSSYHAGAAAL"
     gene            complement(12280..12471)
                     /locus_tag="PLT63_06430"
     CDS             complement(12280..12471)
                     /locus_tag="PLT63_06430"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOD98396.1"
                     /translation="MDKLYLSETDKKIGGVCGGIGEFFGKDATLFRILFILLILFSFG
                     VGVIAYLAMWLVIPKRPQM"
BASE COUNT         2540 a         3686 c         3676 g         2635 t
ORIGIN      
        1 tcttcatgcc cttttcggct tcggaacgga agcgtaccgc ttcggaacga taggcctcca
       61 cctggtagag atcactttcc ttgaccgtcg tggactccag ctccaggagc ttgcggatcc
      121 gctgggccgc cgaggcgaag aaatccatcg actcgtcggc gtagttcagc ccggaactcg
      181 ccagcaccaa cccgtagtag agctgtctga cccggaggac gatctgtccc ttcttgtccg
      241 cgacggcatg ctccttggcc ttgactcccc tggccgccgc ctctttgcgg ttggatatct
      301 tgccgaaggt atagaggggc tgcgtggccg taatatcgaa tttgccgaag atgccgatgc
      361 tcgaaaagct gagccccgga gaaggatcgg ttatcctccc gtttaccacc aacggctcct
      421 tggcgtcccg catcggtccg gccaccgcga tggcctcgat ttggggatag taggccgcct
      481 cgacctgttg gagatcgctg cgggccgcca ggacatcgct ttgcacctcc tcgatttccg
      541 gattttgcgc taccgccagg gcgacgatct gctccaggtt catgaccttc ggactgatct
      601 gctcccggca cagggcacct cggggcagca tccccgccag gcacaggatg atcgccgtca
      661 tgagcagcag atgtttggca ttgatcatga gcgccctcca cttcgaaata gattgcatcc
      721 tacccttcag ccctccccgg tcggtacggc ttttgtacgg ctgtccggca tgatggccca
      781 ataaactccc aggccggcca ccccccagaa gatctgttcc aaccgcaggg cgatcccata
      841 ggtcaggccc agggccgtgg tgaatcccag gacgctgaag accatcacgc ggatactctc
      901 ctggaggccg atttccaggg ggatgaaaaa aaccaggagg tcaagccagg tggccagcaa
      961 ccacaccgcc gccgccgtaa aaaagttgtc gccggtgaac aggctgagga aataccatgt
     1021 tttcaccatg ctgacaacga aaccggttag gtgccagccg atggacgccc agagcccccg
     1081 gggattgtcc cggtagaact gccggagatc ctgatcgatt tcggtgatat atgccactgt
     1141 ccgcttcagg ctttcgagcc ttaggcgggt cgccagccac cggagcgccg cgccgagctt
     1201 cccctggtac tgaacgatca gaaaccccgc cagtcctgcg cccagaaaaa ggtttcccgc
     1261 caggagcgcc caataaccgt atgccggcag cgagatctgc cggatgacga tcagcgagcc
     1321 gatgagggcc gtggcgaact gggagagggc ataggacagc ttgttgatga tgacccccgt
     1381 tgccgcaccc ggtccctgat gttccgctgc gagcagcatg cccttgacaa cctcgccgcc
     1441 caccgtcgcc gtcggggtga agtagtttat ggcgccgccg gccagatcga tattgtagag
     1501 gcgggaaaag ggcagggacg agtgggggtg ggtcagacac tgccgccagg cgacggtgtg
     1561 gaatatcttt accacccctt ccatgaggat gaaggggacg agtccccagc ccaaaagata
     1621 aaaatcgtgc catagcgccg ccggaccgat tttccagatc agaaacacca ggagggaaac
     1681 gccgaggacc aaagccatga gatggaattt cttcatgccg gcattcaagg gacgatcact
     1741 tttccatggc ctgccgctgg aggcgcatct tgtctaggag gactcggaag gaatcccgct
     1801 ggatgacccg ggcgaaggaa cgtcggtagt tttccgtgat gctgatcccg tcgatcttca
     1861 cgtcctggac gagccatttt ccatccacgg gcgccaggga atattccacc gggatctcct
     1921 cgctcatccg gacgatccgg gtcttgatca gactccggtc cggagccacc tcttctctat
     1981 cgtagaggat ccgttcccgc ttcaggaaat ccaagacaag tttcgtgtag gaatcctgga
     2041 acaggtcctg gaagagggca agaaaggacg cccgctggga tgcgtcgagt ccttccccgt
     2101 attttcccaa cgcctggcgc gccatgccgt cgaaatggaa gtttcgggcg atgaccgcct
     2161 ttatcgcccg ccgccgttca tcacgatgct gccgatcctg aagccgggga tcggtctgga
     2221 tggccagcac ctgatcgagc atgtcccgaa ccgattcgcc ggccgttgcc tcccctgggt
     2281 cctgggcgct tccccgggcc gccggaatga ggaccgccgc caggaggagc aggcacacta
     2341 tggtcgaaag ccaacgcatg ttatggttca tatccatttt attcccgcct ttattccgct
     2401 gatctgaagg cgaagtcagc gaagctgatt gatgctcata aatcagcgaa actggttaat
     2461 gttcaagaat cgggaccttc aaggcagcgg tggtccgccg cgagatcccg ccgcattcgt
     2521 tcgggccgcg gggaggtcat gggtaatcca ggcaaataat ccggcgagga gaaaatcgtt
     2581 gcgttgcgtc atgttgcaga ccatgatgat ggaccgtaac gtctggcggg agatctttac
     2641 ctcaccgccg ctacagaaca acttgttttt gttgatgttg cgcagggtga aaacggccat
     2701 gccgatggcc cacagacaga atctcctgat gccgacttcc tccggtggga tcaggagggt
     2761 gtagcttagg gcgttattta ggtgcccccg ggtgatgccg atcatctcgg cgatcccccg
     2821 ggcgaaggcc tccccgccgt cgcccggggt cagcctgttc agatcgcacc ccacggcagc
     2881 aaaaacggaa cggggcagcc agcatgttcc ctggcgctgg tcttcccaaa gatccttgat
     2941 gatattggtc atctgcaatc cctgtccgaa cgaggcagcc agggccagga gttcctggcg
     3001 ccgtccggcg atggcgggcg cgtggtcgca gaacagttcc gtgagcattt cccctactac
     3061 tcccgccacg aaatagcagt agcggttcag ggcctccaga tcttcgagcc cccccggttt
     3121 cttgagccgc tggaactcca gcatgccctg ggccatgacg gtcccgcatt ttcttatcac
     3181 ctgccgctgc ggaggggtca gactgaggtt acccccgatg atcaccgggg tgtttttgac
     3241 cagttccctc tccgcctccg ggacgccgtc cgagagtcgt gccgtcaaat tctccgcgaa
     3301 ggggacggcc ggggcctcgg cattcaggac ttgcacgaat tccttgaaga agcgggcctt
     3361 gacatccaaa ggcagggcct cgtcgtcctc gatggtgtcg atgattcggc agatcagata
     3421 ggcattcgtc accacccggc caagatcggc gggcagttcg ggtatcgtca gggcaaacga
     3481 tcgggacacc ttcttgaggg cttcaaactc gaagtccaga tcaggacggc aaatatccgc
     3541 agcatgcatg tttcctaatt ctccctgctc tctggcaatc gctcccggtg cattggtttc
     3601 gtcgtgaccg gaaaagctca tttgcctgcc gttagcttgc atatctcgcc ggccaatttc
     3661 aagtccataa attacccgag tggtttaaga taaccggcca gggcctggac ggggtgggtc
     3721 atcatatcca tgactgcggt cccttcaaaa ccgctgtgga gcatacagtg ttcgcattgg
     3781 ggattcttgc cgggaccgta atgttcccag gccgtttctt ccatgagggc ccggaaactg
     3841 gccgcgtagc cgtcggcaaa gaggtagcac ggcctttgcc atccaaagat attcctcgtc
     3901 acggtgctcc agggggcgca gtcatagatg cggtttcccg acaaaaaatc gatgaaaaag
     3961 cgggagtggt tgaaccgcca ctttcgtctt cggcccagtc tgaatacctc ccgaaagatc
     4021 tgcttgcttt cagggcgttt gagaaagatc cccttttggg gcgccccggg ataatcgtaa
     4081 ccgggggaaa cggtgatgcc ctcggccccg agatgcatga tgaaatcgaa gaattctccg
     4141 gcttggacgg cgctgacgcc gttgaaaatg gtgcagttga ccgtaaatcg gaagccccgg
     4201 tttttcagga gctgcatggc ccggacggcg gtgtcgaata ccccgtcctt gccgacgatg
     4261 cggtcgtgta gctcccggaa gccgtcgagg tggatggaga aggtcagata gggggaaggg
     4321 cggtaagccg ccagtttctt tttcagaagg atgccgttgg tgcacagata gacgaatttt
     4381 ttgcgcacgg tcagaccggc gactatctgc ggcaagtcag catggatgag cgattccccc
     4441 ccggcaatgg agaccaccgg ggcgccgcac tcttccgctg cggacaggca ctccgccacg
     4501 gagagccgcc gatccagtac ctcctcgggc cagcatatct tcccgcaccc ggcgcaggcg
     4561 aggttgcacc tatacagtgg ttccagcatc aggaccatgg gaaagcggct ccgtccggtg
     4621 gccttttggc ggtatatgta acgggagatg cgcagtttct gaatcagtgg tatgcccatg
     4681 gtcaatcaac gtcccatcga gtcgtccgtc tggccccggg ccagttgagc atggagcggc
     4741 gatattcccc ggagcgccgc tttggcggtg aacaggccgg tctcgtcgtc gatatctaac
     4801 cagaacaggc cggtgacatc cattgcctcc atccttcccc gccgagccag ttcccggaca
     4861 ccttccgaca gactttcccg ctcccgggag acggcggttt ccagggcggg gaagatcccc
     4921 tccgtgcaga gaaatacccc ggtgtcgatg ccgttgtaat aggggatttc tttcccgatg
     4981 tccagcactt gtccgtcgtt ggcctttact ttagtagcat cctccaggtc cggcactccc
     5041 gggggatgga agtccacggc gaggcggcag tggccttcga taaggggggt ggagaacagt
     5101 tttcccagaa gacgcgggtc gaagagatga tccgccatca tgagcgcaaa ggcgtcgggc
     5161 acggcccccc gggccgccag gaccgagatg ccgttcggac gctcccattc agtgttgcgg
     5221 atacagcgaa tgcatatccc ggccggcgtt tcccgggtga gatatgcctc gatatcttca
     5281 ccccggtacc ccgtgatgac ggtgaaactg tctattcccg cccgggcggc gcggccgatg
     5341 acatgcatga tcaagggctg gccgtctatt tcaagaagtg gtttgggacg atgctccgta
     5401 tccgcatgga agcggcggcc gcggccggca gcgatgatca gggcatgatg tatatcggta
     5461 aatgttcggg gttcaatagt cactgaaatt ttccgctcct atctggaaac tgtttctttc
     5521 cgtaaaattg acgacccaag aagcaacggc cgcccgggta aagggcatgg gcagccgggc
     5581 cagcatggaa cggtaactgt agaatttatt gtgcaactcc tcaacatacc gcaaaagatc
     5641 ggcgggagaa aaatggggat gtttgatgtg gcaggacatc cccggccatc ggccgatacc
     5701 gtcgctgtcg atgatccggt tttccgcctc gagccggtca tagagggccg atcccttgtg
     5761 aggggtcagg atgttcagat aggccgccgg caccttgttc ctcatgagga aggaaagggt
     5821 ggccgggaag atatcctcac gatcggcgtc ggagccgaag atgaaattca gggaatagct
     5881 tatcccccgc ttcctcaggt ccctcaggat ctcctcctgg ttgaccatat ttatcttctt
     5941 gttcatttct accagggtat cggcgctgat gctctcgatc ccgatattga cgtggagcag
     6001 accgctgcgt tgggccaagt tcaggaattc cttgtcggcg cagaggcgca gagaccatag
     6061 ggctgaccat ctcaacttca gcgggatgat ggcctccatg agtttcatgg cgttttcgac
     6121 cctgccggca aagttgctgt cggcaaatag gatgtagcgg gaaccgcacc tcttgatctc
     6181 ttccacgacc tcggcgatgg ggcggcaccg gtattgggac ccaagataga gacgctccga
     6241 gcagaattcg catttgaatg ggcatccccg ggaagactgt acggaaaagg tcttgaaata
     6301 tccgtatttg tccatgttca acagtccgta gcggggcccg ggcagccctt ggaggtcgca
     6361 aagtctatcg gagcggtaga gcttccgcag acttccgttt tccgcatcgg cgagcatttg
     6421 ccgccatatg ttttcccctt cgccgatgcc cacggcgtcg cagtgttcgg acgcctcgtc
     6481 gccgtggaag gacatgtggg ggcctcccat gatgacgggc aaaccccgct gccggaaacg
     6541 gtcggcgatg tcgtaagccc ggagggaatt gatcgtccag accgtaatgg ccaccagatc
     6601 ggccggggcg gcgaagtcta ttccgtcgag ctgctcgtcc aagagcttca cctcccagtc
     6661 ggcgggggtg agcgccgcca gataaggcag ggtgagtggg acgaccttgc gcttgtcaat
     6721 cttgaccacc gtccgttcgg agcggctccg gtagtgggac ggttggatga tgaggagttt
     6781 ctttcccatg gccagggtcg tcaaccttcg ttttctcaac gggtcggcat ttccgaatcg
     6841 gttcgggctg ccgccgagac attcttttcg gcgcccgtcg ccatccagag tgcggcggcg
     6901 aagatatagc tgccgaaggc cgccccgata aggaaccagt tcaacctccc caccgcggca
     6961 agcaggacca tcaggtaggc aaaatcacgg ttgctcatca tggtcaggag ccgccccagg
     7021 gcgggggatc ggaaagattc cacctccggt ttgaggaggc gcagatacac taccgcgccg
     7081 cacagcgcga accctcccaa aaggacggcg aggagctgaa tatagagatg atcccccgtc
     7141 tgccggtaac accccgcggc cagaccggag aagatcgcca catggacgat gttgtcggtg
     7201 acgatgtcaa gcttgtggcc gaactcgctt tcctggagtt tcatgcgcgc tatttctccg
     7261 tctatgccgt cgattatgat gcagaacaga aagaggagac agccggcaag ctggtgccaa
     7321 tagccgccct gggcgataaa aaacgcgccc agcagtccga ttcccgcccc caccagggtc
     7381 acctggttgg gggtcactcg ggtcagggcc agccgggtgc tcatcattag ggagatatgc
     7441 ctgctcacat gccgggacat gaatccatcc gtggccttgg tctgggccct gatggcgtcg
     7501 accagcttgc gttctgcgtt tttgccgttt ccggcgccct tcaggcggca gggaagtccg
     7561 tttttaccac tgaggataga gaattctgaa caggtatcac cgctgttgcc gcccaatgtc
     7621 caggcttgct tcattacggc ggcgaggttc cggtaagagc tcaggaatat ccttgcccca
     7681 ctcccgtcgg attcgagaca ggggatttgc ggttcctcgc ccatctgcgc cagattttgc
     7741 agattggcct tatcgacaac caaatcggcc ctcatgacga ggatctttcc tccctccgga
     7801 cgcggatggc tcgccaggat ggcgttggcc gcatcgtcca gttcccccgg gtctcccact
     7861 ggatggaaac catctggtgc ggccagatcg gacaacagct ccttgaaatg atccgtaccg
     7921 ccaacgatat ggatttcacc gaatccgatt tgggtgcaca ggagggcaag ccgccgcgcc
     7981 gccgggacgc cgaacacctt ttttatcccc tccgtgtcgg gggtgaggat tatggagatt
     8041 tttccttctt tttgttcttg gtccgtcatt ttctatctgc atgctttttt caaactctat
     8101 ctcgctatca caaaccaccc tcgatggcaa agaaaaaggt ccgtaacttg tcttttctct
     8161 cttccttttc ggctcggttt catcgtgagg caaagggacg gcggcaatgt ggtccgcaag
     8221 cggaagcgga cgctccggaa aatcacgcgg aatagtttgc cggtgcagag attaaggcca
     8281 tcgttttgaa aggttggcat attgcgttat ggctctccgg cgtttttccc ttgacacctg
     8341 acaatcccaa gttatatggg atcagggaca agaacaggcg agggggatat ggaaacgggg
     8401 atgaataaaa tcagaatcgc tctgatcgga atcggcaact gcgcctcagc gctgatccag
     8461 gggattgaat attacaaaga taaatcgccc caagaactca tcggcctgat gcactggcaa
     8521 atcggccagt atcgtccggg tgatatcgaa gttgtggccg ccttcgacat cgacgagcga
     8581 aaagtaggtc gggacgtatc cgaagccgtc ttccagcttc ccaattgcac tacggttttc
     8641 aatccccata tccccgccgc cggtgtcatc gtccatatgg gaaggattct ggacggcatg
     8701 gccgatcata tgaacgatca tgaggacaaa tacacctttg ttccggccag acggaaagag
     8761 gcgacgaaaa aggatatcgt tgccgaactg aagcgaacgg gggcggaaat cctcatcaat
     8821 tacctgccgg tggggtcgga aaaggcgtcc cgctactatg cagaatgcgc cctggcggcg
     8881 ggagtagcct tcattaattg cattcccgtg ttcattgcca gcgatacccg gtgggcccgc
     8941 cgttttacag agcggaatct gccgattatc ggcgacgacg tcaaatccca gatgggcgcc
     9001 actgttatcc accgggtcat gacccatctt ttccaggccc ggggcgtcaa actcgagaag
     9061 acttaccagt tgaacaccgg cggcaatacc gactttctca acatgcttga ccgcagtcga
     9121 ctgcaatcca agaaggaatc caagaccgaa gccgtgcagt ctcagaccaa aaagcgcctc
     9181 gagagcgaaa acatccatat cggtcccagc gattgggtgc cctggcagaa ggacaacaag
     9241 gtctgtttcc tccgcatgga ggggaagatc ttcggcaaca tccccatgaa tctggaaatg
     9301 cgtctttccg tggaagattc ccccaattcc ggaggcgtca tcatcgatgc catccggtgc
     9361 tgcaagctgg ccctcgacag ggggaagggg ggggtgctgt tctctccttc cgcttatttc
     9421 atgaagcatc ctcctcggca gtttttcgac gaagacgcct accagatgct cgaggacttt
     9481 atcagggaaa aaagggacag ctaaatcaac ccgaggacat tacagacgca agacctcgga
     9541 aagggtcggg tgagggagta tccatccccg gagggttttc aaatcggcgc cgacggtcat
     9601 ggcgagcgtc agggcggcga tgaggtccgg agcaccttcc ccggcgatgg tggcaccaac
     9661 gaggcggcct gcgtggaaga tgaacttggc gaagcccgga cccgccagtt ccgtgcgggc
     9721 catgatgttg acaccgtagt cggtgcggcg tatctctacg ttccagccat tctccctggc
     9781 ctgccgctcc gtgaggccga tgcgggcgat ggggggatgg gtgtaggtta cggaggggac
     9841 catctcctcg cgatatacca ccgtaccgtc accgaagagg cgactggcga gggcgcgccc
     9901 ctgctgctgg gcccgatggg cgagcatgat accgccggtg acatccccga tggcatagat
     9961 cccggcgata ttggtttcct ggtgttcatt gacggcgatg ccgcgccggt cgtaccggat
    10021 atccatctcc cgcagcgact cctcgtcgat ccgcggccgt cgccccgtgc agaccagggc
    10081 gtattccgcc tctaattcca acctgttccc gtcccgatca ccgccaacga tgactccgcc
    10141 tcccttttcc tccagcgagc cgaccgacag cgccgtgtgg attttgatgc ccatcttttg
    10201 cagttccctg gccaggaact ccgccgcctc ctcttcctca taggggagaa tgctgggaag
    10261 aatttccacg atttccactt ttgtgcccac ctcggcaagg aatgtggcga attcgacccc
    10321 gatgacgctt ccgccgatga tgacaacgct ttgcggcaac gccgcaagct ggaggaaggc
    10381 atcggaagtc aggactcttc ccggaatccg gccgggtacg tcgtctggtg atcgccgccc
    10441 gtcgtttgca gatgccgccg caactgaaga caatgccccg ctttctgaaa ttagcgcttc
    10501 gctacgagag gaggttccac gacgggacgc cggtgattcc ctaccgtcaa tgtcctgttt
    10561 cgggagcagg aacggaggca gggagggctc tgagccccag gcgatgacca gatgcgatgc
    10621 cgccagggtt tcgctttccc cggtggcgct tgtccactgg acctttcccg aagacaggat
    10681 gcggccgtac ccgtttctca tggttactcc ggctgcttgc agggtttttt ccacccccag
    10741 ggcggagatc ctggtcatct ggtcccggtg gcgccgaatg acggcgtaat cgaagcccgc
    10801 ctcacccagg gagatcccga agcgcttaat cttcttcagg tccacgtaat gtctgctgca
    10861 gacaagcagc gccttggtgg ggacacagcc ccggtgggtg cacgtccccc cccagccgtt
    10921 ccgctcgatg atgcagactc gggcaccctg aagggccgcc tcttccgcca ccgcatgacc
    10981 gccggggccc ccaccgataa cgatcaggtc aaaactttcc attgacatga caacccctct
    11041 tttccacttg aatattcgtt tctacattca ctaatagttt tcctcacctt tatataataa
    11101 tttcttgccg caagtacggg aatgactttg cgtctgcctt tgccggaatg gaaatcccct
    11161 ggattcccgt gtgtgcggga acggcagaat ggggcattat gcaagctatc tcaacggtta
    11221 cggtaggtta tggcgtccat gatcctttgc acctgccgtg ggtgcaaaat tattttcccg
    11281 gcaatgtccc ggggcggcga tcagagcgcc gccgcaccgg catgatacga actccggacc
    11341 atgggccccg cctcgacgtg gggaatgccc agcgccaggg cctcttcccg aatggcggcg
    11401 aactcatcgg ggtggtagta cttccggact ggggcgtggc accgcgtcgg ctgcaggtac
    11461 tgtcccactg tcaccgcctg gcagcggacg gcggcaagat ccgccaggag ccgccggatg
    11521 tcctccccgg tttccccaaa accgatcatg aagcctgatt tggagcacga agccgtcttt
    11581 cccctgctga ggtgccggag gatttccagg gactgcgcat agttgccctg ggggcgcaga
    11641 tcggtgaaca gagagggcac cacctctata ttgtggttga tcatgtcggg tccggcggcg
    11701 atcactttct caagatcctc tttatgaccc tgaaaatcgg ggatgaggac ctcgatacgg
    11761 atagcaggaa gcgccgcccg cagggacgta acagcgttgg caaagactgc ggcgccgccg
    11821 tcgtaaaggt cgtcccgggt gacggaggtg aggacggcgt acttcaggtt cagatattct
    11881 acggccgcca caaggcgcgg aatctctccg ggatcgaccg ggccggggac tccgtgtttg
    11941 acgttgcaat agcggcaatt gcgggtgcag acgtcgccca ttaggaggaa ggtggccgtg
    12001 ccgtcctgga aacattccgc catgttaggg caccgggcct cctggcagat ggaatggagg
    12061 ccgtggcggg ttagggtatc ccggacgcgg ctgaaccctt tcgtgccggg cagtcttact
    12121 ttcagccagg ggggcttgcg caggtattcc ccgccttccg cctccggaag actccttgct
    12181 tctttcacca ctgcttcctc tccggcggcg ggaacccgtt gcgggggctg gtcaagccgg
    12241 cgcttctctg ctgtggccgc atgatgatct ccttatccct cacatttgcg gccgcttggg
    12301 gatcaccagc cacatggcga ggtaggcgat gacgccaact ccgaaggaaa acaggatcaa
    12361 aaggatgaag aggatccgga acagggtggc atccttgccg aaaaactccc ctatgccgcc
    12421 gcagacgccg ccgattttct tgtcggtttc ggatagatac aacttgtcca taactgtctt
    12481 ttctcccgta taaatatgat gccctagcaa aaagtgattt tcttcctccc cctgcaa
//
LOCUS       DAOSMF010000051        12503 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_5865_length_12503_cov_25.061456, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000051 DAOSMF010000000
VERSION     DAOSMF010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 12503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12503
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5865_length_12503_cov_25.061456"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="PLT63_06435"
     CDS             <1..594
                     /locus_tag="PLT63_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98397.1"
                     /translation="ADITEAAMKLLQGGFEQLRLYFLVGLPTETAADIEAVIELIWRL
                     ARITTTTAGPVEKRFQRLTVSINQFIPKAGTPFQWHPLEDIQTIRKKIQSIAGAFRGR
                     GNIRIAAEPAKSNYLQALFARGDRRVGRFLLALHNLEGNWMRARKEAALDPDFFVYRL
                     RGATEILPWDFIATGVPKTLLRKEFEAAQPMAANKIY"
     gene            642..717
                     /locus_tag="PLT63_06440"
     tRNA            642..717
                     /locus_tag="PLT63_06440"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:675..677,aa:Ala,seq:ggc)
     gene            complement(846..1388)
                     /locus_tag="PLT63_06445"
     CDS             complement(846..1388)
                     /locus_tag="PLT63_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98398.1"
                     /translation="MKRILIIALGIIMLATGVAMADSKPIQLSLTPGIAIFDRNTTIE
                     GFSLGIWSENPQKAFALGFANGSTGNSAGFSLGFLLNYADSYKGVQWALVNYTQKSFV
                     GWQSGFVNYTDDAFKGLQTGFVNYAGQLTGLQLGFFNYAAKASSGVQLGVINIIPQNK
                     WFTNFPNELAPGMIIFNWRF"
     gene            complement(1568..2536)
                     /locus_tag="PLT63_06450"
     CDS             complement(1568..2536)
                     /locus_tag="PLT63_06450"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOD98399.1"
                     /translation="MKWTGKRVGLALGGGGVRGFSHIGVLKVLEQEDIGIDLIAGTSA
                     GALIGGAYAGGLSPREIHRRVESYLQSPAFKNSALQALGSTVNPRKKNALEKASHLLR
                     TRYLMVRAIFHPAILPSVEFESLVDYFIADINIEDTRIPFLAVTTDLISGERVVFKEG
                     SLRKAVLASCAVPGAVEPIRQGEWLLADGGITSLVPVHAVKQADVDVVIAVVVDRDAR
                     GAPVFETAQDIFQRAGEIAADKLEAIELQDADVIIRPEVGDLHWMDFSRSRDLIHAGE
                     VAARASLARIHQALPLPRRLARLARLAFPFGQKNVRKATIKAGERK"
     gene            complement(2612..5437)
                     /gene="uvrA"
                     /locus_tag="PLT63_06455"
     CDS             complement(2612..5437)
                     /gene="uvrA"
                     /locus_tag="PLT63_06455"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016099277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOD98400.1"
                     /translation="MEFIRIKGASQHNLKHVNIDIPRDKMVVITGVSGSGKSSLAFDT
                     IYAEGQRRYVESLSAYARQFIGQMDKPEVESIEGLSPAIAIEQRTAGQNPRSTVGTVT
                     EIYDYLRLLFARIGVPHCYQCGREIRSQTIDMMVEAILAFPDRTKFNILAPVIRGKKG
                     EFHKELKKLLKDGFVRIRVDGELRELGEEIVLDKQKRHDIEVIVDRLVRKDGIRKRLR
                     DSLETAAHLAEGLVSVQIAGGEELLFSEKYACPECGISVPELAPRMFSFNSPYGACNE
                     CDGLGSKMFFDERLVVPDGGLSLREGAIVPWAGRDSLSYHQMLDSLAGHYGFDINTPF
                     AELPPPVQKALLYGSGKEKIRFYTDRRGRRYFYSKSFEGVLNELNRRYLETQSNAVRM
                     ELSRYINLRECPACHGARLKRESLAVTVGGRNIDDLCRLSIREVCEFFSGLSLSTKEV
                     QIAERILKEIRERLNFLLDVGMDYLSLDRPSGTLSGGEGQRIRLATQIGSGLVGVLYV
                     LDEPTVGLHQRDNMRLIATLKKLRDLGNTVLVVEHDADMMLAANQIIDMGPGAGLDGG
                     EVVFQGSPEEIRRDGKSLTGQYLAGGKAIPLPTARRVASDRQIVIEGAHEHNLKNVDL
                     RVPVGLFTVVTGVSGSGKSTLIIETLYKAMARRINRQRGGMGKIGRIADLGGIERVIL
                     MNQQPIGRTPRSNPVTYTGVFTHIRDLFTGLPEARIRGYKPGRFSFNVKGGRCESCEG
                     NGLIKIEMHFLPDVYVTCDVCRGRRFNQDTLDIYYREKNIADVLNMTVQQALGFFENI
                     PAIRQKLQLLADVGLGYIRLGQSATTLSGGEAQRIKLSRELGKRANSNTLYILDEPTI
                     GLHFDDVQKLLTVLMRLVDMGNTVVVIEHNLDIIKSADHIVDLGPEGGPGGGRIVATG
                     TPEEVAANTASMTGQFLKKTLAQASGG"
     gene            complement(5443..6411)
                     /locus_tag="PLT63_06460"
     CDS             complement(5443..6411)
                     /locus_tag="PLT63_06460"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOD98401.1"
                     /translation="MKKIYLIMIAAILAIALSVPAAGEVVDRIVAIVNDDVITLSELN
                     ENFDPYQKKIEASLTGQEKERMIAEGKTTILKRLIDSKLIEQEAKKSGLTVKDDEVMG
                     FVKEVLKGRNMQMSELIQALNKDGLTFEGYKQELREQMMRQRLIRKEIRSKIVITDDE
                     IGDYYRQHRDEYEGKEAVRLKQILIPVPAMADDLLRKRAREVAEGALKRIKGGESFET
                     VAVQFAQGSAGAAGGDIGFVEKGSMLPEVEKAALNLDIGVVSSVIETPQGLYIIQVVD
                     KRGAGLKPITEVRQEIRMKLENEKATVKFEEWIAELRAKSLVEVKL"
     assembly_gap    6593..6692
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6693..9943)
                     /gene="mfd"
                     /locus_tag="PLT63_06465"
     CDS             complement(<6693..9943)
                     /gene="mfd"
                     /locus_tag="PLT63_06465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOD98402.1"
                     /translation="MVRKIVRPAMRSPDPALEELLARIEEREKVVSVQGLEATARAFL
                     MALLHRRITRPLLIVAPTEKEARQLGRDLSFFSGAGQVFLFPPWDAVSTDLFAWQREV
                     ELARCEGLCRLLTGECPLMVVPVQALLQKLPSRQTISAYLNRVALGDFLDRDEWVLHL
                     LQGGYHRVTLVEGKGEFAVRGHVVDLFPPLAPHPYRLEFVGDEIEAIREFAPASQRSR
                     REIDEFLLTPAREIIMTESRQALGIRNARLRAGDLGLPRLVRSRLLERMADGLAMSVN
                     PLLFPLFYEGETPPGMLLDYLSPDSLLIWDDPLAIDRSGEELANETDRLLLKARREER
                     FYLEREAHYWTGAELDRTGEPFQHLHIHSLVMGRQPAGVIFRTVGMTGIKSGIQIRDG
                     ERGVLSPLVERLKAWMEEGYQIFFVCRGREGRQRLAQLFAQHDLTFTGLPAEGPMGPE
                     PAPGVPDAAGADRNGLTGDAAFLDVFPIPSGTAGSLRIMEGNLATGFVYDALGLVVLS
                     DEELHGKKIWGRRFRPTREGYFLKSFGELREGDHVVHKEHGIGRYGGLQKLTVEGVEN
                     DFLLIEYLDQDKLYIPVDRLDRIQRYLGPDGATPRLDRLGGTSWEVVKERVKKSIRET
                     AEELMAIYAAREVMDRRSFSPPDGLYNDFCAAFEYEETPDQDRAIEDIHLDMSRTKPM
                     DRLICGDAGFGKTEVALRAAFRAVMEARQVAILVPTTILAEQHYQTFSRRFRPYPVRV
                     EVLNRFRTKSEQAGIVAEINRGTVDIVVGTHRLLQKDVRFRELGLVIIDEEQRFGVQH
                     KEKLKKLRTLVDVLTLTATPIPRTLHLSLVGIRDLSIIHTPPEDRQPIKTHVIEFNED
                     IIREAILWELDRQGQVFFVHDRVRSIYSMARFIEKLVPRARVAVLHGQMKAAEIETTM
                     VRFIQQQCNVLVCTTIIGSGVDIPTANTIIINRADRFGLAQLYQIRGRVGRAREEARA
                     YLLVPKGAMLSRDAQKRLEVIMEFSEPGAGFRIAANDLEIRGGGSLLGVSQSGHVTAV
                     GYELYTELMEKTVRELKGETVPEEEVRPEINLGLPALIPADYMPDEQRRLVTY"
     gene            complement(9950..10861)
                     /gene="nadA"
                     /locus_tag="PLT63_06470"
     CDS             complement(9950..10861)
                     /gene="nadA"
                     /locus_tag="PLT63_06470"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOD98403.1"
                     /translation="MNQKELQEEIRRLLREKNAILLAHYYERDEVQEIADYRGDSLGL
                     SIEAARTDAETIVFAGVHFMAESASILSPDKTVLLPRQEAGCPLADMVTAPQLAAARE
                     KHPEAKVVTYINSAAIIKAHSDICCTSANALRVVGSLPGAGPILMIPDGNLARYVARQ
                     TGRQIIPWEGCCPVHDRLTAAAVRAVRGLHPDAVFAAHPECKGEVLDLADFVGSTTGI
                     IGYAGRPEVTKLIVGTERGVFYELQTRYPTKTFIPAAEDFLCLDMKLTTLEDIHAALI
                     ERRYIVKVPEAVRIPARRALDRMLELS"
     gene            11131..12366
                     /locus_tag="PLT63_06475"
     CDS             11131..12366
                     /locus_tag="PLT63_06475"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HOD98404.1"
                     /translation="MIPVDEARQIILDGINPLPLEKVNILEALGRVIGEDVAAPRNLP
                     PKNNSAMDGYAVRAEDTKAATTQNPIVLTVTEDIPAGTIPKQPLGAGQAARIMTGAPL
                     PGGADAVVRMENTRKDGGKVHILAAAVSGLDIRVAGEDVAAGETVVGRGTVIRPAEVG
                     MLAALGRSFVYVHQRPTVAILATGDELVDIDEPPSDWKIISSNSYATAAQVLESSAAI
                     LQLGIVKDCREDLISKFREASRADLIISSGGVSVGDYDLVKDIMKEVGNTMQFWQVAM
                     RPGKPLAYGAIQGVPVFGLPGNPVSSMVSFEQFVRPSLLKMMGHKNIFRRQIRAILED
                     DLEKMAELRYFIRGWVRREAGRYYVRSTGEQGSGILKSMVRANALIVLPEAETSIARG
                     TEVQVQLLDTSLEHADRPD"
BASE COUNT         2436 a         3789 c         3637 g         2541 t
ORIGIN      
        1 gccgacatca ccgaggcggc gatgaagctc ctgcaagggg ggttcgagca gctccggctc
       61 tatttccttg tcggcctccc cacggaaacg gcggctgata tcgaggccgt catcgagcta
      121 atatggcgtc ttgcccggat caccacaaca acagccggac cggtggaaaa acgtttccaa
      181 aggctcaccg tcagtatcaa ccagttcatt cccaaggcgg ggacgccttt tcagtggcac
      241 cccttggagg acatccaaac gatccggaag aagatccagt ccattgccgg ggccttccgg
      301 ggccggggca acatcaggat cgctgcggaa ccggcaaaaa gcaattatct ccaggccctc
      361 ttcgcccgcg gcgaccggag ggtggggcgg tttctcctcg ccctccataa tctggaaggc
      421 aactggatgc gcgcccgtaa ggaagccgct ctcgatcctg attttttcgt ttatcgcctc
      481 cggggggcaa cggagatcct cccctgggat ttcattgcca ccggcgtccc caaaaccttg
      541 ctacgtaagg aatttgaagc tgcgcagccc atggcggcga ataaaatcta ttgacaatat
      601 ttttccggct gtgttatggg gcgggctcga attgcccatg tggggttgta gctcagttgg
      661 gagagcgctt gaatggcatt caagaggtca ggggttcgac tcccctcagc tccaccaaga
      721 aaatcaaggg gttgcggatc gttaccgcaa cccctctttt tttcccacag agattcagga
      781 tcggggccac ggctgatcgc acccccccaa aaagcctcca gtgaagttat cttgatcgct
      841 acggtttaaa aacgccagtt gaagataatc attcccggcg ccaattcgtt gggaaaattt
      901 gtaaaccatt tattctgggg gatgatgttt atgacgccga gctgcacccc ggaagaggcc
      961 ttggcggcat aattaaaaaa accgagctga agtcccgtaa gctgcccggc atagttcacg
     1021 aaaccggtct gcaggccctt aaaggcatca tccgtgtagt tcacgaaacc cgattgccat
     1081 ccgacgaagc ttttctgcgt gtaattgacc agcgcccatt gcacaccctt ataactatcc
     1141 gcgtagttga ggagaaaacc caggctgaaa ccggcgctgt taccggtaga tccattggca
     1201 aagcccaggg cgaacgcttt ctgcggattt tcgctccaaa taccgaggct gaaaccttca
     1261 atcgttgtat ttctgtcaaa aatggcaata cccggggtaa ggctgagttg aatcggtttc
     1321 gagtcagcca tcgccacacc ggtcgcaagc atgatgattc ccagcgcaat gataagtatc
     1381 cttttcatat tccccttccc ctttcattga tgaatttcaa caacggcctc tgccaacaca
     1441 tacaattggc actacggatt ctttccgacg tttcctctat tgctaatttt aataccatat
     1501 tattccctcg gtgttcaagc cggattttat cgaaccgtcc gggtgttttt cggctgaaca
     1561 tatcagtcta ttttctctcc cctgccttaa tggtcgcctt ccggacgttt ttttgaccaa
     1621 agggaaaggc caacctggcc aggcgggcca gacggcgcgg caggggcaaa gcctgatgga
     1681 tgcgggccag actggctcgg gccgccacct ccccggcatg aatcagatca cgggatcggc
     1741 tgaaatccat ccagtgcaga tcaccaactt ccggtctgat gatgacatcg gcatcctgaa
     1801 gttcgatggc ctccaacttg tctgcggcga tctcccccgc ccgctggaag atgtcctggg
     1861 cggtctcaaa aacaggggcg ccccgggcat cccgatccac taccacggca attaccacat
     1921 ccacatccgc ctgtttcacc gcatggacgg gaaccaggct ggtgatgccg ccgtcggcca
     1981 gcaaccattc tccctggcgg atgggctcca ccgctccggg aacagcacag ctcgccagca
     2041 cggccttgcg cagggaacct tccttgaaca ccaccctttc cccgctgatg agatccgtgg
     2101 tcaccgccag aaagggaatg cgggtgtctt cgatatttat gtcggcgatg aaataatcga
     2161 ccagcgactc gaactccacc gagggcagaa tcgccggatg aaaaatagcc cgtaccataa
     2221 gatagcgggt tctcaggagg tgggacgcct tttcaagggc atttttcttc cgggggttga
     2281 cggtgctgcc cagggcctga agggcggagt tcttgaaggc aggactctgc aaatagcttt
     2341 ccacccgccg gtggatctcc cggggggaca ggccgccggc ataagcgccg ccgatgaggg
     2401 ccccggcgct ggtgccggca atgaggtcga tgccgatatc ctcctgctcc aggaccttga
     2461 ggacgcctat gtgggagaac ccccgaacgc cacccccgcc tagagccaat cccacccttt
     2521 tccccgtcca tttcatcttg accgcctttt tttacgattc tctaagctct tcccgtacca
     2581 attgccaaag cggaactgtt tttccggaat ctcaacctcc agaggcctgc gcaagcgttt
     2641 tcttcaggaa ttgccccgtc atggacgctg tgttggccgc cacctcctcc ggggttcccg
     2701 tggccacgat cctcccgccg ccgggaccgc cttcgggacc cagatcgacg atgtggtcgg
     2761 cggacttgat aatgtcgagg ttatgttcga tgaccacgac ggtgttgccc atatccacca
     2821 ggcgcatgag gacggtcagg agcttttgga catcgtcgaa atgcaggccg atggtcggtt
     2881 cgtctagaat gtagagggta ttgctgttcg cccgctttcc cagttcccgg gagagtttga
     2941 ttcgctgggc ctcgcccccg gacagggtgg tggcggactg gccgaggcgg atatagccga
     3001 gtcccacatc ggctaaaagc tgaagtttct gccggatcgc cgggatgttc tcgaagaatc
     3061 ccagggcctg ctgaaccgtc atgttcagga catcggcaat attcttttcc cggtaataga
     3121 tatccagggt gtcctgattg aagcgtctgc cccggcagac atcgcaggtg acatagacgt
     3181 cgggaaggaa gtgcatctcg atcttgatga ggccgttgcc ctcacaggat tcgcaacgcc
     3241 cacccttgac gttgaagctg aaacggcctg gtttgtaacc ccggattcgc gcttccggca
     3301 gccccgtaaa gaggtcgcgg atgtgggtga agaccccggt ataggtcacg gggttggaac
     3361 ggggggtcct gccgatgggc tgctggttca tgaggatcac ccgctcgatc cccccgagat
     3421 cggcgattct gccgatcttg cccatccctc cccgctgccg gttgatccgc cgggccatgg
     3481 ccttgtatag ggtctcaatg atcagggtgc tcttccccga gccggacacc cccgtcacga
     3541 ccgtgaagag ccccacgggc acccggagat ccacattctt caggttgtgc tcatgggcgc
     3601 cttcgatgac gatttgacgg tccgatgcca cccttctggc cgtcggcagg ggaatggctt
     3661 ttccccccgc gagatactgg cccgtcaggg acttcccgtc ccggcggatc tcctcggggc
     3721 tcccctgaaa caccacctcc ccaccgtcca gacccgcccc cggacccatg tcgatgatct
     3781 gattggcggc cagcatcatg tcggcgtcgt gttcaaccac caggacggtg ttgcccaggt
     3841 cgcggagctt tttcaaagtg gcgatgaggc gcatgttgtc ccgctggtgc agccccaccg
     3901 tgggctcgtc gaggacatac aggacgccca cgagcccgga gccgatctgg gtggccaagc
     3961 ggatccgctg gccctctccg cccgaaaggg ttcccgatgg tctgtccagg ctcaggtaat
     4021 ccatccccac atcgaggaga aaattgaggc gctcccggat ctccttgaga atccgctcgg
     4081 cgatttgcac ctcctttgtc gagagggaaa ggccggaaaa gaattcgcat acctcccgaa
     4141 tggacaaccg gcagagatcg tcgatgttcc ggcctcccac cgtgacggcc aggctttccc
     4201 tttttagccg cgccccgtga caggcggggc attcccggag attgatgtat cgggaaagtt
     4261 ccatgcggac cgcattggac tgggtctcca gatagcgccg gttcaactcg ttcaggacac
     4321 cctcgaagga cttggaataa aaataacggc ggccgcgccg gtcggtatag aaacggatct
     4381 tctctttgcc cgatccgtac agcagggcct tctgcacggg cggcggcagc tccgcgaagg
     4441 gggtgttgat gtcgaaaccg taatgcccgg ccaaggaatc gagcatctgg tgataactca
     4501 gggagtcgcg gcccgcccag ggaacgatgg ccccctcccg gagggaaagg ccaccgtcgg
     4561 ggacaacgag acgctcgtca aagaacatct tgctgcccag accgtcgcat tcattgcagg
     4621 ccccgtaagg gctgttgaag gaaaacatcc gcggggccag ttccggaacg ctgataccgc
     4681 attcggggca ggcgtatttt tcgctgaaga gtagttcctc tccgccggcg atctggacgg
     4741 aaactagacc ttcggcaagg tgggcggccg tttccaggga atctcggagg cgcttccgga
     4801 tgccgtcctt acggacgagc cgatcgacga tcacctcgat atcatgacgc ttctgtttat
     4861 ccagaacgat ttcttccccc agttccctga gttcgccgtc cacacggatg cggacaaagc
     4921 cgtccttgag cagtttcttc agttccttgt gaaactcccc cttcttgccc ctgatgacgg
     4981 gggccaggat gttgaatttg gtccggtcgg gaaaggcaag gatggcctca accatcatgt
     5041 cgatggtctg ggaacggatc tcccggccgc actggtagca atgagggacg ccgatccggg
     5101 cgaagaggag gcggagatag tcgtatatct ccgtgaccgt gccgacggtg gaacggggat
     5161 tctgaccggc ggtgcgctgc tcgatggcga tggccggcga cagtccctcg atggactcta
     5221 cctccggctt gtccatctgc ccgatgaact gccgggcgta ggcagagagg gactccacat
     5281 aacgccgctg gccctcggca taaatggtgt cgaaggcgag ggaggacttg ccggagccgc
     5341 tgaccccggt gatgaccacc atcttatccc gggggatgtc gatgttgacg tgtttgagat
     5401 tgtgttggga agctcccttg atcctgataa actccataaa gctcagagct tgacctcgac
     5461 cagggatttg gcgcggagtt cggcgatcca ctcctcgaat tttacggttg ccttctcgtt
     5521 ttccagtttc atccggatct cttggcggac ctcagttatg ggcttcaatc ccgccccccg
     5581 cttgtcgacg acctgaatga tgtataatcc ctggggagtc tcgataacgg aactcacgac
     5641 gccgatatcc agattcaagg ccgccttttc cacttccggc agcatgctgc ccttttccac
     5701 gaagccgatg tcaccaccgg ccgctcccgc cgatccctgg gcaaactgca ccgccaccgt
     5761 ctcgaaactc tctccgcctt tgatcctctt gagagcgcct tccgcaacct cccgggcccg
     5821 cttgcggagc aggtcatccg ccatggccgg cacggggatc aggatctgct tcagccgcac
     5881 cgcctccttg ccctcgtatt catctcgatg ctgccgataa tagtccccta tttcatcatc
     5941 ggtaatcacg atcttggaac ggatttcctt gcgaatcagg cgttgccgca tcatctgttc
     6001 ccggagttcc tgtttgtagc cctcgaaggt gagaccgtcc ttgttcaggg cctgaatcag
     6061 ctccgacatc tgcatgtttc tgcccttgag aacttccttg acgaacccca tgacctcgtc
     6121 gtccttgacg gtaaggccgg attttttcgc ctcctgctcg atgagcttgc tgtcgatcag
     6181 ccgtttcagg atggtcgtct ttccttccgc gatcatcctt tccttctcct ggccggtcag
     6241 actggcctcg attttcttct gatagggatc gaagttttca ttgagttccg aaagggtgat
     6301 tacatcgtcg ttcacgatgg ccacgatgcg atccaccacc tcgccggccg cgggaacgga
     6361 cagggcgatc gccagtatcg ccgctatcat gatcagatat atcttcttca tgttttcacc
     6421 tgctttctcc taatcctcac cgcctctacc acgatcagaa ttattttgcc atagttaagg
     6481 acccttcgcc ccgcaaggcg cgcaacagtt ccttgacggc ccccgtaagc atcgatcccg
     6541 ccaatccggg aaggggcacg atgagcttga aatccggcgt cagtttcacc ccnnnnnnnn
     6601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnttataggt cacgagccgc cgctgttcgt
     6721 cgggcatgta atccgcgggg ataagcgccg gcagccccag attgatttcc ggccggactt
     6781 cctcttccgg aacggtttcg cccttgagtt cccgcaccgt cttctccatg agctccgtgt
     6841 acagctcgta ccccaccgcg gtgacatggc cggattgcga aacccccagg agacttcctc
     6901 ccccgcggat ttccaggtcg ttggcggcga tccggaagcc ggccccaggc tccgaaaatt
     6961 ccatgatcac ctccaggcgc ttctgggcgt cccgggagag catggccccc ttgggaacca
     7021 aaagataggc ccgggcctcc tcccgggcgc ggcccacccg gccccggatc tgatagagct
     7081 gggccaggcc gaaacggtcg gcgcgattga tgatgatcgt attggcggtg gggatgtcca
     7141 cgccggaacc gatgatggtg gtacaaacca ggacgttgca ttgctgctgg ataaaacgga
     7201 ccatcgtcgt ctcgatttcc gccgccttca tctgcccgtg gaggaccgcc acccgggctc
     7261 ggggaacgag cttttcgata aaccgggcca tggaatagat agatcggacc cggtcgtgga
     7321 caaaaaatac ctgcccctgc cggtccagtt cccacagaat ggcttcccgg ataatatcct
     7381 cattgaattc gatgacatgg gtcttgatgg gctggcggtc ctccgggggg gtgtggatga
     7441 tggacaggtc gcggatgccg acaagggaga gatggagggt tcggggaatg ggcgtggccg
     7501 taagggtcag gacatccacc agggtgcgga gcttcttgag cttttcctta tgctgtaccc
     7561 cgaagcgctg ttcctcgtcg atgatcacca gtcccagctc ccggaagcgc acgtcctttt
     7621 gcaggaggcg atgggtcccc acaacgatgt ccaccgtacc cctgttgatt tccgctacga
     7681 tcccggcctg ttcggacttg gtgcggaaac ggttgaggac ctccacccgg acgggataag
     7741 gacggaagcg ccgggaaaag gtttgatagt gctgttccgc caggatcgtc gtcggcacga
     7801 ggatggcgac ctgcctggcc tccatgacgg ctcggaaagc cgcccgcaga gcgacttccg
     7861 tcttgccgaa cccggcgtcg ccgcagatga ggcgatccat gggctttgtc cggctcatat
     7921 cgagatggat gtcttcgatg gcccggtcct ggtccggggt ctcctcgtat tcgaaggcgg
     7981 cgcagaaatc gttgtacagc ccgtcgggag gggagaagga ccggcggtcc atgacctccc
     8041 gggcggcata gatcgccatt agctcctccg ccgtttcccg aatggatttt ttcacccgct
     8101 ccttgacgac ttcccaagag gtgccgccca ggcggtcgag acgcggcgtc gccccgtccg
     8161 ggcccagata tctctggatc cggtccaggc ggtccaccgg gatatagagc ttgtcctgat
     8221 cgagatactc gatgaggaga aaatcgtttt ccacgccttc cacggtgagt ttctggaggc
     8281 ctccgtaacg gccgatgccg tgttccttgt ggaccacgtg atctccttcc cggagttcgc
     8341 cgaaggactt gaggaaatag ccctcccggg tgggtcggaa gcgccgcccc caaatctttt
     8401 tcccgtggag ttcctcatcg gagagaacga ccagccccag ggcgtcgtaa acaaaaccgg
     8461 tggcaagatt gccttccatg atccggaggc tgccggccgt accggaggga atggggaaga
     8521 catcgaggaa ggccgcatcg ccggtgagcc cgttccggtc cgccccggcc gcgtcgggga
     8581 cgccgggagc gggttcggga cccatgggac cttccgccgg caggccggtg aaggtaaggt
     8641 cgtgctgggc aaaaagctgg gcgagtcgct gccgtccctc ccggccgcgg catacgaaga
     8701 agatctgata accttcttcc atccacgctt tcagtctttc caccaagggt gaaaggaccc
     8761 ccctctcacc atcccggatc tggatgcccg acttgatgcc agtcatcccc accgtgcgga
     8821 agataactcc cgccggctgc cgacccatga ccaggctgtg gatatggagg tgttggaagg
     8881 gttctccggt ccggtcaagc tcggcacctg tccagtagtg ggcctccctc tccagataaa
     8941 aacgctcttc ccggcgggcc ttcaagagca gccggtcggt ctcgttggca agttcctccc
     9001 cggaccgatc gatggcgagg ggatcgtccc agatcagcag actgtccggg gagagataat
     9061 ccagcagcat ccccggcggc gtttcgccct cgtaaaagag gggaaagagc agggggttga
     9121 cggacatggc cagtccgtcg gccatgcgct ccaaaagccg ggaccgcacc agccggggca
     9181 atcccaggtc gccggcccgg agccgggcgt tacggatgcc cagggcctgc cggctctcgg
     9241 tcatgataat ttcccgggcc ggcgtcagaa ggaactcgtc gatctcccgg cgggagcgct
     9301 gggaggcggg agcgaactcg cggatggctt cgatctcgtc accgacaaac tccagacggt
     9361 agggatgggg tgccagaggg gggaacagat caacgacatg gccgcgtacg gcgaactccc
     9421 ccttcccttc caccagggtt acccggtgat aaccgccctg gaggagatgg aggacccatt
     9481 catcgcggtc gagaaaatcc cccagggcaa cccggttcag ataggcggag attgtttgac
     9541 gggacgggag tttctgcagg agggcctgca cggggacgac catcagcggg cactcgccgg
     9601 tcaagagccg gcaaagacct tcgcatcttg ccagctccac ctcccgctgc caggcaaaga
     9661 gatccgtgga taccgcgtcc caggggggaa agagaaagac ctgtccggct ccggaaaaaa
     9721 aggacaggtc acggccaagc tgccgggcct ccttttccgt cggggcgacg atcagcaggg
     9781 gccgggtgat ccgccggtgc agcagggcca tgaggaatgc ccgggccgtc gcctccaatc
     9841 cctggacgga aacgaccttt tcccgctcct cgatccttgc cagcagttcc tccagggccg
     9901 gatcggggct ccgcatcgcc ggccggacga tctttctgac catgagcggc taagaaagct
     9961 ccagcatccg gtccagggcc cgccgggcgg gaatccggac cgcttccgga accttgacga
    10021 tataccgcct ctcgattagg gcggcatgaa tgtcttctag ggtggtcagt ttcatatcca
    10081 ggcagaggaa atcctccgcg gcggggatga aggtcttggt cggatagcgg gtttgcagct
    10141 catagaagac gcccctttcc gtgccgacaa tgagcttcgt cacctccggg cgcccggcgt
    10201 agccaatgat gccggtggtg gagccgacaa aatcagccag atcaaggacc tcgcccttgc
    10261 actcgggatg ggcggcgaag acggcgtccg gatgcagccc ccggacggcc cgcaccgccg
    10321 cggcggtgag ccggtcatgg acgggacagc agccctccca ggggatgatc tgacggccgg
    10381 tctgccgggc aacgtaacgg gccagattac cgtcggggat catgagaatc ggccccgcgc
    10441 cggggaggga accaaccacc cggagggcgt tggcggaggt gcagcagatg tcgctgtgcg
    10501 ccttgataat cgccgccgaa ttgatgtagg tcacgacctt cgcctcgggg tgtttctccc
    10561 gggcggcggc aagctggggc gccgtcacca tgtcggccag gggacagccg gcctcctgtc
    10621 gcggcagcag aaccgtcttg tcgggggaga ggatggaggc gctttccgcc atgaaatgca
    10681 ccccggcaaa gacgattgtc tcggcgtccg tccgggcggc ctcgatgctc aatcccaggg
    10741 aatcgccgcg ataatcggct atttcctgca cctcgtctcg ttcgtagtag tgggccagga
    10801 ggatggcgtt tttttcccgc agcagtctgc ggatctcttc ctgcagctct ttctgattca
    10861 tggactttca actccctggg ggtcgtcccc gagcttgaca ctggcatccg aacaacatct
    10921 gcggacacgc cccttttttc tatcgcggag aactctctat aatataaaca cctttacttc
    10981 aagggcaaac atgccgtcgc ccagccggag ccgacgcggc cattctcccg cccccgccgc
    11041 cttccgtcga ctccggcccc gagcgcggag ccgcacccgg ctttgtcggt aatagttgac
    11101 aaggccctcc atttaagata gcttcccagt atgattcctg tagatgaagc ccggcagatc
    11161 atcctcgacg gcatcaaccc gctacccttg gaaaaggtaa atattctgga ggctctgggg
    11221 cgggtcatcg gcgaagatgt ggcggcgccc cgcaatttgc cccccaagaa caactccgcc
    11281 atggacggat acgccgtccg cgccgaagac accaaagccg ctacgaccca aaatccaatt
    11341 gtccttaccg tcaccgaaga tatccccgcc ggaacgatcc ccaaacaacc gctgggggcg
    11401 ggacaggcgg cgcggatcat gaccggggcg cccctgccag gcggggccga cgccgtggta
    11461 cgcatggaga acacgcgcaa ggacgggggg aaggttcata tcctggccgc ggcggtctct
    11521 ggactggata tccgcgtggc cggcgaggat gtcgccgcgg gggaaacggt agttggccgc
    11581 ggtacggtta tccgtcccgc ggaagtggga atgctcgccg cgttaggcag atcattcgtc
    11641 tatgttcatc agcgccccac cgtggcgatt ctggccaccg gcgacgagct cgtggacatc
    11701 gacgagccgc cttccgactg gaaaatcatc agcagcaaca gttacgccac cgccgcccag
    11761 gttctggaaa gcagcgccgc gattctccaa ttgggaatcg tgaaagactg tcgggaggac
    11821 cttatctcca aattccggga ggcatcccgg gcggatttga tcatctcctc cggtggcgtc
    11881 tccgtaggcg actacgatct ggtcaaggac atcatgaaag aagtgggcaa caccatgcaa
    11941 ttctggcagg tggccatgcg gcccggcaag cccctggcct atggcgccat ccagggggtg
    12001 cccgttttcg gtttgcccgg caatcccgtt tcgtccatgg tctcctttga acagttcgtt
    12061 cgtccctccc tgctcaagat gatggggcac aaaaatattt tccgacggca gatccgggct
    12121 atcttggaag atgatcttga aaaaatggcc gagttgcggt attttattcg cggctgggtg
    12181 cgccgggagg cggggcgata ctacgtaagg tcaacggggg aacaaggatc gggaatcctc
    12241 aagtccatgg tccgggccaa cgctctcatc gtcctgccgg aggcggagac atcgatcgcc
    12301 cggggaacgg aggttcaggt gcagctcctg gacacatccc tggagcacgc cgatcgtcct
    12361 gattaagggg catagaagct tgaaggcgct atcgctctca ttgcataatc gggcgccgga
    12421 aacattgtaa aaagcagcat tttccaaacg gaagtgccaa ggtcactggt gggcctcccg
    12481 gacttcaaat ccggtgtggg gcg
//
LOCUS       DAOSMF010000052        12340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_5945_length_12340_cov_23.450631, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000052 DAOSMF010000000
VERSION     DAOSMF010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 12340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12340
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5945_length_12340_cov_23.450631"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            241..864
                     /locus_tag="PLT63_06480"
     CDS             241..864
                     /locus_tag="PLT63_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HOD98405.1"
                     /translation="MKALRKLYDWVLHWADTPYGAPALFILSFMESSFFPIPPDPLLI
                     ALVLGGRDKAFRFAANCTAASVLGALFGYAIGYYVWWSGPDKFSAVAWFFFQHIPGFS
                     VELFRYIQGLFEKWNFWIIFTAGFTPIPYKVFTVSGGAFNVNLPMFLIASIISRGARF
                     FLVAFLIWKFGPLIKSFIDRYFNWLAVLFTILLIGGFLLVKWWGASL"
     gene            1022..1630
                     /locus_tag="PLT63_06485"
     CDS             1022..1630
                     /locus_tag="PLT63_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOD98406.1"
                     /translation="MKSLDKRKEIISVAMELLAENGFHGVPMSMIADRANVAVGTIYR
                     YFAGKDELINALFQELEARITAAIRGSFPTTGSFRERFLYLNGIILRYLIDNPLHFRY
                     LEQFMNSPYGVSMRRDRLLEKEGRDDIFMTLFEEAIAARIVKDIPINALFSLAIAPLI
                     FLVRDHILGFVTLNEDLIRQTAEACWDAVRRNDCESGADRAR"
     gene            1703..2911
                     /locus_tag="PLT63_06490"
     CDS             1703..2911
                     /locus_tag="PLT63_06490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOD98407.1"
                     /translation="MTTHEQIKSIAAGMLCGLLILTGCGQGGNTGKGRAGGPPEVAVV
                     TVQPQQVLVTTELSGRTSANLIAEVRPQVGGIIQQRLFTEGSDVKAGQVLFKIDPAHY
                     QAVYDNAKAALGRSEANLSAIKLRAERMRELVAEKAVSQQEYDDATAALKQNQADIQF
                     WKATLETASINLKYTSVTAPISGRIGKSNVTVGALVTAHQPVPLATIQQLDPMYVDVP
                     QATTELLRMRRHLAEGRLHRNGQLRNKVQILLEDNTRYPYDGALQFRDVTVNQTTGSV
                     ILRVIVPNPKGVLLPGMFIRAVVEEGINKNALLVPQQGVSRDPKGNPYCLIAGAAGKV
                     EQRPITIERAVGNQWLVTEGLAPGEQVIVEGILRTKPGATVKVVPFVDNAGKTGGETG
                     PAPPAAAKAN"
     gene            2924..>3543
                     /locus_tag="PLT63_06495"
     CDS             2924..>3543
                     /locus_tag="PLT63_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOD98408.1"
                     /translation="MLSKFFLERPVFAWVIAIVMMVLGGLAIYNLPISQYPPIAPPSI
                     AIYSLYAGASAETVENSVTQIIEQKMTGFDDMLYISGTSDSSGASRIELTFKPGTDPD
                     LAWAKVQNKLQLAMTSLPEVVQRTGVTVSKSTRNYLIIVGLISEDGSMDGTDLRDYAQ
                     SNLEKVLARVPGVGEVQIFGGQYAMRIWLNPDKLNDYRLTMEDVIQA"
     assembly_gap    3544..3643
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3644..>4926
                     /locus_tag="PLT63_06500"
     CDS             <3644..>4926
                     /locus_tag="PLT63_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOD98409.1"
                     /translation="VRVAIKEVVQTLFEAILLVFLVMWLFMGNLRATLIPTIAVPVVI
                     LGTFAVLGAFGFSINMLTMFAMVLAIGLLVDDAIVVVENVERIMTDEGLPPREATAKS
                     MEQITPALIGIGLVLAAVFGPMAFFPGSTGVIYRQFSVTIAASMLLSVVVALILTPVL
                     CATLLKPVAKGHEAAEGAVFFLRPFFLWFDRAFFGLRDRYVQVVGRVLSRKKRYLAVF
                     AVIVVLLGALFMRMPTSYLPDEDQGILLCQILLPTGSTLEQTQEVADQVQRHFHENEK
                     EAVESCMTIIGIGFSGRAQNNGMVFVRLKDWHLRDRDDLKVKAIAGRAMGAFAKIRNA
                     MVFAFPPPAVVEMGNARGVDFQLLDRGGLGHGALMNARNQLLGMAAKDPRLANVRPNG
                     MEDIPEFRIDVDWEKTGAMGIPLPSVHNTIAAAFGG"
     assembly_gap    4927..5026
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5027..5218
                     /locus_tag="PLT63_06505"
     CDS             <5027..5218
                     /locus_tag="PLT63_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98410.1"
                     /translation="LVVIFAPLFYVVIERLFGKHNHGAAVVSGGLGSLLKKLAGRLPG
                     KRGPGKTPENDGDIVTEDR"
     gene            5220..6644
                     /locus_tag="PLT63_06510"
     CDS             5220..6644
                     /locus_tag="PLT63_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HOD98411.1"
                     /translation="MKRSRMVFTGCLSVLVIAMVLLNGCTLAPKYTRPAAPMPADWPT
                     GEAYGKEAAAPGNRAAAEMKWEEFFTDPELRQVIAAALANNRDLRLASLNVEKARALY
                     GIQRAELFPAFNAVGAGSKGRTPADLSYSGTETTYEQYSVNFGVMAWEIDFFGRIRSL
                     KDRALEEYLATDQARRGAQILLVSAVANAYLNLAADRESLKLTTSTLAAQEGAYKLIK
                     KRYDVGLASEIDLRRAQSQADTARGDVARFTQLVAQDENALQLLVGAPLTVLKLPSEL
                     GGLNPFRDISPGLSSEVLLSRPDILAAEHRLRAANASIGAARAAFFPRISLTTSFGTA
                     SADLSGLFKDGSSTWNFAPQIIMPIFDARLWSAYDATKVEKEISLTQYERAIQTAFRE
                     TADALAVRGTVNRQLAAQKSLVEAFAETYRLSAIRYAKGVDSYLSVLDAQRSLYGAQK
                     GLIGIQLLRLANEVALYKALGGGA"
     gene            6708..7523
                     /locus_tag="PLT63_06515"
     CDS             6708..7523
                     /locus_tag="PLT63_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOD98412.1"
                     /translation="MESSSPQTLPLWTRPFAAVGRRAIRWVNHLGAVAIFFILATLKV
                     VHPKQLAKIAYQIYEIGARSVPIILLVGLFTGMVLGLQTYHALVTVGAQGALGTLVAL
                     SLVRELGPVLTAIMITARAGSAITAEIGIQRISEQIDALDTMHIDPLRYLISPRIVAA
                     VISFPILTALFDIIGIIGGYFSGVVLMGANAGAYIYRVQASMELKDITDGFIKAVVFA
                     VIVTAVCCYQGYFAHMREDSRGAKAVGLSTTAAVVISCVLILVADYIVTSLLI"
     gene            7534..8394
                     /locus_tag="PLT63_06520"
     CDS             7534..8394
                     /locus_tag="PLT63_06520"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOD98413.1"
                     /translation="MDAPLIEFQDVTKSFGPRTVLDRVNLRIHAGQVTTIIGLSGAGK
                     SVLLKHIIGLLKPDSGRILFQGRPLDEMTGSERKSSLAQISYMFQDNALFDSLSVYDN
                     IALPLRETTDLSRSEINRRVKARVEQMELADAVNKYPSELSGGMQKRAALARALVTDP
                     RIVLFDEPTSGQDPIRKNAILGMIAQYQRRIGFTAILVSHEIPDVYFISNRILALYDR
                     TVVFQGTPAELEDFDHPFLDDIIRSLEGLHRELTDPYARTRFAMAHRELEKRRTGKDI
                     HVLPAENHHG"
     gene            8437..8925
                     /locus_tag="PLT63_06525"
     CDS             8437..8925
                     /locus_tag="PLT63_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990531 - phospholipid-translocating
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005548 - phospholipid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HOD98414.1"
                     /translation="MQKYKLETVVGVFLVLGLVCIGYMTVKLGHVSLLGDDSYPLSAR
                     FTSVTGLRAGSVVYIAGIEVGRVERLYIDQENQQAVVEMRIRKDIRIYDDAIAAVKTE
                     GLIGDMHLRIDPGGGGDLLKSGGVITDTQPPLDIGELVGKYAFGDVKKPGDENKEQKE
                     KP"
     gene            8922..9518
                     /locus_tag="PLT63_06530"
     CDS             8922..9518
                     /locus_tag="PLT63_06530"
                     /inference="COORDINATES: protein motif:HMM:NF017319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOD98415.1"
                     /translation="MSKKTFAVLGIILMLLSALPVHAGAPRNTVEENVNKVLSILRDP
                     KLKGEGAKETKKARLRALYDVMFDEIELSRRTLARNWNKFAPAERKEFVLLFKQVLEK
                     AYIDKILSYTNEKIVFDREITRGENLAEVQSRIIASGKEIPISYRVIMKNNTWKVYDV
                     VVENVSLVQNYRTQFNGILAKSTPEELLVTLRKKVKGP"
     gene            9554..10609
                     /locus_tag="PLT63_06535"
     CDS             9554..10609
                     /locus_tag="PLT63_06535"
                     /inference="COORDINATES: protein motif:HMM:NF016236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaA family lipoprotein"
                     /protein_id="HOD98416.1"
                     /translation="MIPALRSCLIAALAFVFLAGGCAHGPKQAPESPAPAALTRVTAD
                     PAVVTAAPAPESGASARPKDKEITAAAPAPRIAANSPNAAPLPPVAKATNPGPDPSSS
                     EPGAQADTEETSGEADEGLEGDFIATDDEGEADSAAGPREEALTIADPWEPFNRAMYH
                     FNDNLYFWVLKPVAQGYAKALPEGVRISIRNVFVNIAFPARFVNCVLQLDLPCAAVET
                     GRFVINSVWGLGGLFDLASDEAINLPKQRRDLGQTLGVHGIGPGFFINWPIFGPSSPR
                     DTVGLMGDILMDPLAWVSPWYAATGIAAGYKLNETSLSIGDYEALKEAAIDPYVAIRD
                     AYVQYRYHKIKQVTDRK"
     gene            10677..>12340
                     /locus_tag="PLT63_06540"
     CDS             10677..>12340
                     /locus_tag="PLT63_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HOD98417.1"
                     /translation="MKFLPSVVSYFLKGESTRQNIRMLLRFFLALFVMITAYSVIFHL
                     LMAAEGQRHSWITGLYWTMVVMSTLGFGDITFHSDVGRIFSILVLLSGVIFLLILLPF
                     AFIKFFFAPWIEAETRKRYAQELPPGTKNHVIITAYDQVTMALIEKLGIYKRDYVVIV
                     DEYERSKELYDTGVRIAVGHIDDPETYRKMRVEQAAMVVATNRDEMNTNIAFTVRETT
                     ETVPVVATSDSPHSVDILYLAGCTRVLELTEILGRSLAGWTLGGDFRANVLGRFDNLI
                     IAEAPAISTPLVGKTLAESRLREEIGVTVIAVWERGRFVAPTPEAAINRSTALVFAGT
                     EEQIARYDEVYGFYQLTRHAGDPVIIIGGGRVGKAIAQRFKEREVDYLIVEKNPRRTE
                     DDTCYITGDAADIGTLKRAWIEKTPAALITTHDDATNIYLAKYLRSLRPDMQIICRAN
                     TDRNISTLHRAGADFVMSYASLGANAIFNYLRKEDMFMLAEGLNIFTIPLPPALAGKT
                     LAQSRIRPKTGCSVIGIRRGDNVAINPDPFLPVEADSELILIGSPEA"
BASE COUNT         2417 a         3981 c         3530 g         2212 t
ORIGIN      
        1 gctgaagaag atggccaatt agattgcctt gacgaaaaaa atgcgctatg gctggtcgcg
       61 ttggtgcagg tgggaaagat gggctgaccc cgccccaaac cgcgggacat ataggcttcc
      121 tcagacaaag gggaccggcg gccgcaactt ccggggcgga ctagggatcg cattgccggg
      181 gcaaaacgag atagaagaag tgacgttcac aagttcgtta taaccgatac actcctaacc
      241 atgaaggcgc tgcggaaact ctacgactgg gttctccact gggccgacac cccctacggg
      301 gcgccggccc tgttcatttt gtcctttatg gaatcctcct tctttcccat cccgccggac
      361 cccctcctca tcgccctggt cctggggggg cgggacaagg ccttccgctt cgccgccaac
      421 tgcaccgcgg cctcggtgtt gggggcgctg ttcggctacg ccatcggcta ctacgtctgg
      481 tggtccggcc cggataagtt ctccgccgtg gcctggttct tcttccagca catcccggga
      541 ttttccgtgg agctgttccg ctacatccag ggcctgttcg aaaaatggaa tttctggatc
      601 atcttcaccg ccggcttcac ccccatcccc tacaaggtgt tcaccgtctc cggcggggcc
      661 ttcaacgtca acctgccgat gttcctcatc gcctccatca tcagccgcgg ggcccggttc
      721 tttctggtcg ccttcctgat ctggaagttc ggacccctga tcaaaagctt catcgaccgc
      781 tacttcaact ggctcgccgt cctttttaca attctcctca tcggcggctt cctcctcgtc
      841 aagtggtggg gcgcgagcct gtagcggcat gaatttcccc gtccacgcca tcccgaatta
      901 caccggaaaa gaggcagggg aggcgacgaa cgggaaaaaa atcttgacac cagggaggat
      961 atggcataca agcggcgagc ctgaatgaac gttcattcgc ttccaccata acatttgaga
     1021 catgaaaagc ttggacaagc gcaaagagat tatttcggtg gccatggaac tcctggcgga
     1081 aaacggtttc cacggggtgc ccatgtccat gatcgccgat cgggctaatg tggccgtggg
     1141 gacgatctac cggtatttcg ccggcaagga cgagctgatc aatgccctct ttcaggagct
     1201 ggaagcaagg ataactgccg ccatacgggg aagttttccg accaccggct cctttcggga
     1261 gcgatttctc tatctgaacg gcatcattct ccggtatctg atcgataatc cccttcattt
     1321 ccgctatctc gaacaattca tgaattcacc ctacggggtt tccatgcgcc gggacagatt
     1381 gctggaaaag gaaggccgtg acgatatctt catgaccctt ttcgaagagg ccatcgccgc
     1441 ccgcatcgtc aaggacatcc ccatcaacgc ccttttttcc ctggccatcg cgccgctcat
     1501 ctttctggtc cgggaccata ttctgggttt cgtcaccctg aacgaagacc tgatccggca
     1561 gaccgccgaa gcttgctggg atgccgtccg gaggaacgat tgcgagagcg gggccgatag
     1621 ggcccggtaa tggccggacg gccgcgacgg tgtggagaca aaacgatgaa acaccaaaaa
     1681 ttacccctta ccgaggtgac gtatgaccac tcatgagcag atcaaatcca tcgccgccgg
     1741 gatgctgtgc ggactcctga tcctcaccgg ctgcgggcaa ggcggcaata ccggcaaagg
     1801 tcgggccggc ggtccccccg aggtggccgt cgtgacggtg caaccccagc aggtgctggt
     1861 caccaccgag ttgtccggcc ggacctccgc caatctcatc gccgaggtgc ggccccaggt
     1921 gggaggcatc atccagcagc gcctgttcac cgaaggatcg gacgtcaagg ccggacaggt
     1981 cctcttcaag atcgaccccg cccactatca ggccgtctat gacaacgcca aagcggccct
     2041 cggccgctcc gaggccaacc tctcggcgat caagctccgg gccgaacgga tgcgggaact
     2101 cgtcgccgag aaggcggtca gccagcagga atacgacgac gccacggccg ccctgaaaca
     2161 gaaccaggcg gatatccagt tctggaaggc gacccttgag acggcgagta tcaaccttaa
     2221 atacacctcc gtcaccgccc ccatctcggg acggatcggc aagtccaacg tcacggtggg
     2281 cgccctggtg acggcccatc agcccgtgcc cctggccact atccagcagt tggaccccat
     2341 gtacgtcgat gtcccccaag ccaccaccga gctgctacgg atgcggcgcc acctcgccga
     2401 ggggcgtctc catcgcaacg gacagctccg gaacaaggtc cagatcctcc tcgaagacaa
     2461 cacccgttat ccctacgacg gcgccctgca gttccgggat gtcaccgtca atcaaaccac
     2521 aggatcggtc atcctccggg tgatcgtacc caatccgaaa ggtgtcctcc tccccgggat
     2581 gttcatccgg gcggtggtgg aagaggggat caacaaaaac gccctcctgg tgcctcagca
     2641 gggagtatcc cgggatccca agggaaaccc ctactgcctc atcgccggcg ccgcaggcaa
     2701 ggtggagcag cgtcccatca ccatcgaacg ggccgtgggc aatcagtggc tcgtgaccga
     2761 ggggctggcg ccgggcgagc aggtcatcgt cgaggggata ctgcggacca aacccggcgc
     2821 caccgtgaaa gtcgttccat ttgtcgacaa cgcgggcaaa accgggggcg aaaccggacc
     2881 ggcaccgccg gccgcagcca aggcaaactg acggagggca ccgatgctgt caaaattctt
     2941 cctggagcgc cccgtttttg cctgggttat cgccattgtc atgatggtcc tggggggact
     3001 ggccatctac aacctcccca tctcccagta tccgcccatc gccccgccat ccatcgccat
     3061 ttattcctta tacgccggcg cctccgccga aacggtggaa aacagcgtca cccagatcat
     3121 cgaacagaag atgacgggct tcgacgacat gctctacatc tccggcacca gcgattcctc
     3181 cggggcctcc cggatcgagc tgaccttcaa gcccggcacc gaccccgacc tggcctgggc
     3241 gaaggtgcag aacaagctcc agctcgccat gacgagcctc ccggaagtgg tgcagcgcac
     3301 gggggtcacg gtgagcaaat ccacccggaa ctacctgatc atcgtgggcc tcatctccga
     3361 agacggcagc atggacggca ccgatctcag ggactatgcc cagtcgaacc tggaaaaggt
     3421 cctggcccgg gttcccgggg tcggcgaggt ccagatcttc ggcggccagt acgccatgcg
     3481 catctggctc aaccccgaca agctcaacga ttaccgcctc accatggagg acgtgatcca
     3541 ggcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngtgcggg tggccatcaa
     3661 ggaggtcgtc cagaccctct tcgaggcgat cctcctggtc tttctcgtca tgtggctctt
     3721 catgggcaac ctccgggcca cccttatccc cactatcgcc gtgcccgtgg tgattctggg
     3781 aaccttcgcc gtcctcgggg ccttcgggtt ctccatcaac atgctcacca tgttcgccat
     3841 ggtcctggcc atcggcctgc tggtcgatga cgccatcgtc gtcgtggaaa acgtggagcg
     3901 gatcatgacc gacgagggcc tcccgccccg ggaggccacg gccaagtcca tggagcagat
     3961 caccccggcc ctgatcggca tcggcctggt cctggccgcg gtcttcggcc ccatggcctt
     4021 tttccccggc tcgacggggg tcatctaccg ccagttctcc gtcaccatcg ccgcctccat
     4081 gctcctgtcg gtggtggtgg ccctgatcct caccccggtg ctctgcgcca ccctcctgaa
     4141 accggtggcc aagggacatg aagcggcgga aggcgccgtc ttctttctgc gcccgttttt
     4201 cctgtggttc gaccgcgcct ttttcggcct ccgggaccgc tacgtccagg tggtggggcg
     4261 cgtcctgtcc cggaaaaagc gctacctggc cgtattcgcc gtgatcgtgg ttctcctcgg
     4321 ggcgctcttt atgcggatgc cgacgagtta cctccccgat gaagaccagg ggatcctcct
     4381 ctgtcagatt ctgctgccca cgggttccac cctggagcag acccaggaag tggctgacca
     4441 agtccagcga catttccatg aaaacgaaaa agaggcggtg gaatcgtgca tgaccatcat
     4501 cggcatcggt ttttccggcc gggcgcagaa caacggcatg gtcttcgtaa gactcaagga
     4561 ctggcacctc cgggaccggg atgatttgaa ggtcaaggcc atcgccggcc gggccatggg
     4621 ggccttcgcc aagatccgca atgccatggt cttcgccttt ccgccccccg ccgtggtgga
     4681 gatgggcaat gcccgggggg tggatttcca gttgctggac cggggcggcc tcggtcacgg
     4741 cgccctgatg aacgcccgga accagctcct cggcatggcg gccaaggacc cgcggctggc
     4801 caacgtccgt cccaacggca tggaggatat cccggagttc cgcattgacg tggactggga
     4861 aaagacgggg gccatgggca tccctctgcc ttccgtccac aataccatcg ccgcggcctt
     4921 cggaggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnctgg tggtcatctt
     5041 cgcccccctc ttctatgtgg tcatcgaaag gcttttcggt aagcacaatc acggggcggc
     5101 ggtcgtctcc ggggggctcg gatcactgct gaagaaactg gcgggacggc tcccgggaaa
     5161 acgagggccg ggaaagaccc cggaaaatga cggtgacatt gtcacggagg atcgctaaga
     5221 tgaagagaag ccgaatggtc ttcaccggtt gtctgtctgt cctggttatc gccatggtcc
     5281 tcctgaacgg ctgcaccctg gccccgaaat acacccgtcc cgcggccccc atgcccgcag
     5341 actggccgac gggggaggcc tacggcaagg aggccgccgc gccgggaaat cgggcggccg
     5401 cggagatgaa atgggaggaa ttcttcaccg atccggagct gcgccaggtc attgccgcgg
     5461 ccctggccaa caaccgggac ctccgcctgg cctccctgaa cgtggaaaag gcccgagcgc
     5521 tctacgggat ccagcgggcc gaactctttc ccgccttcaa cgccgtcggc gccgggagca
     5581 aggggaggac gccggccgac ctttcctact cgggcacgga gacgacatac gagcagtaca
     5641 gcgtcaattt cggcgtcatg gcctgggaaa tagacttttt cggccggatc cgcagcctga
     5701 aggaccgggc cctggaggaa tatctggcca cggatcaggc ccgccgcggc gcccagatcc
     5761 tcctggtgtc cgccgtggcc aacgcctacc tcaacctcgc cgccgaccgg gaaagtctca
     5821 agctgaccac ctcgaccctg gcagcccagg agggggccta taaactgatc aagaagcgtt
     5881 acgacgtggg attggcgtcg gaaatcgacc tccgccgcgc ccagagccag gcggataccg
     5941 ccaggggaga cgtcgcccgt ttcacccagc tcgtcgccca ggacgagaac gccctgcagc
     6001 tcctcgtcgg cgctcccttg accgtcctga agctgccgtc ggagttaggc gggctgaatc
     6061 ccttccggga tatttccccc ggcctatcct ccgaagtcct cctgagccgt cccgacatcc
     6121 tggcggcgga acaccgcctt cgggccgcca acgccagtat cggcgccgcc cgggccgcct
     6181 tcttcccccg catctccctg acgaccagtt tcggcaccgc cagcgccgat ttgtccgggc
     6241 tcttcaagga cggctccagc acctggaact tcgcgcctca gatcatcatg ccgattttcg
     6301 acgcccgcct ctggtccgct tacgacgcca ccaaagtgga gaaggaaatc agcctgaccc
     6361 aatacgagcg ggcgatccag accgccttcc gggaaaccgc cgacgccctg gccgtccggg
     6421 gcacggtaaa ccggcagctt gcggcgcaga aatccctggt ggaggccttt gccgaaacct
     6481 atcgcctttc cgccatccgc tatgccaagg gcgttgacag ctacctgagc gtcctcgatg
     6541 cccagcggtc cctctacggc gcccagaagg gattgatcgg catccagctc ctccgcctcg
     6601 ccaatgaagt ggccctgtac aaggccctgg gcggaggggc ttgaaaaaat caccttaata
     6661 tgatcttaga gaagaggaaa agacggacga cttcatgaga ggacggaatg gaaagcagca
     6721 gcccgcagac attaccatta tggaccaggc cctttgccgc cgtagggcgg agagccatcc
     6781 gctgggtaaa ccatttgggt gccgtagcga tcttcttcat cctcgcgacg ctgaaggtcg
     6841 tccaccccaa gcagttagcc aagatcgctt atcagatcta tgaaatcggc gcccggtccg
     6901 tgccgatcat cctcttggtg ggtctcttca ccggcatggt cctggggctc caaacctacc
     6961 acgccctggt gaccgtgggc gcccagggcg ccctggggac cctggtggcc ctgtcgctgg
     7021 tccgggagct gggaccggtg ctcaccgcca tcatgatcac ggctcgggcc ggctcggcca
     7081 ttacggcgga aatcggcatc cagcgcatct cggaacagat cgacgccctg gacaccatgc
     7141 atatcgatcc cctccgctac ctcatcagcc cgcggatcgt cgccgccgtc atcagctttc
     7201 ccatcctcac cgccctgttc gacattatcg gcatcatcgg cggctacttc tccggggtcg
     7261 tcctcatggg ggccaacgcc ggcgcctata tctaccgcgt ccaggccagc atggaactga
     7321 aggacatcac cgacggcttc atcaaggcgg tggtcttcgc cgttatcgtt acggccgtct
     7381 gctgctacca gggctacttc gcccatatgc gggaagacag ccgcggcgcc aaggcggtgg
     7441 gactctcgac gaccgcggcg gtggtcatat cctgcgtcct catcctcgtg gcggactata
     7501 tcgtcacctc cctgttgatt tgaggcagac gccatggacg cccccctgat cgaattccag
     7561 gatgtcacca aaagcttcgg accccggacg gttctggacc gcgtgaacct ccggatccac
     7621 gccgggcagg tgacgacgat catcgggctc agcggggccg gcaagagcgt cctgctcaag
     7681 catatcatcg ggctcctcaa accggactcc ggacggatcc tctttcaggg ccgacccctg
     7741 gatgaaatga cgggaagcga acggaaatcc agtctcgccc agatcagcta tatgtttcaa
     7801 gacaacgccc tcttcgattc cctgtctgtt tacgacaata tcgccctgcc gctgcgggaa
     7861 accacggatc tttcccggtc cgagatcaac cggcgggtaa aggccagggt ggagcagatg
     7921 gagcttgccg acgcggtaaa caaatatcct tccgagcttt ccggggggat gcagaaacgg
     7981 gcggccctgg cccgggccct cgtcacggac ccccggatcg tccttttcga cgaacccacc
     8041 tcgggacagg atccgatccg caaaaacgcg atcctgggca tgatcgccca gtaccagcgc
     8101 cggatcggct tcacggcgat cctcgtcagc cacgagattc ccgacgttta cttcatctcc
     8161 aaccgcatcc tggccctcta cgaccggacg gtggtctttc agggaacgcc ggcggagctg
     8221 gaggatttcg atcatccctt cctggatgac attatccgca gtctggaagg gctgcaccgg
     8281 gaactgacgg acccttacgc ccgcacacgg ttcgccatgg cccaccggga actggaaaag
     8341 agaagaaccg gcaaagacat tcacgtcttg cccgcggaaa accatcacgg atgaacagcc
     8401 ggacggcgcc gcgccgcaag gccgggagga attgacatgc agaaatacaa actggaaacc
     8461 gtagtcggcg tctttctcgt cctgggactc gtctgcatcg gctatatgac ggtaaaatta
     8521 ggacatgtat cgctcctggg cgacgattcc tatcccctgt ctgcccggtt tacctccgtt
     8581 acggggctcc gggcgggcag cgtggtctat atcgccggga tcgaggtcgg ccgggtggag
     8641 cggctgtaca tcgatcagga aaaccagcag gcggtggtgg aaatgcggat caggaaagat
     8701 atccggattt acgacgacgc catcgccgcc gtcaagacgg aaggattgat cggcgatatg
     8761 cacctccgca tcgatcccgg cggcggcggg gacctgctca agtcgggggg tgtcatcacg
     8821 gatacgcaac cgcccctgga tatcggcgag ctcgttggca aatacgcctt cggcgacgtg
     8881 aagaagccgg gggatgaaaa taaggagcaa aaagagaaac catgagcaag aagacttttg
     8941 cggtcttggg catcatcctg atgctgctgt ccgcgctgcc tgtccatgcg ggggcgcccc
     9001 gaaacaccgt ggaggaaaac gtcaacaagg ttctgtccat cctccgcgat ccgaagctca
     9061 agggggaggg ggccaaagaa accaaaaagg cgcgactgcg ggccctctat gatgtcatgt
     9121 tcgacgagat cgagctgtcc cggcggaccc tggcccggaa ctggaacaag ttcgcccccg
     9181 ccgaacggaa ggaattcgtc ctgcttttca aacaggtgct ggagaaggcc tatatcgaca
     9241 agatcctctc ctacaccaac gaaaagatcg tcttcgaccg ggagatcacc cggggggaaa
     9301 acctggcgga ggtgcagtcg cgcattatcg cctcgggaaa ggaaatcccc atctcctacc
     9361 gggtgatcat gaaaaacaac acctggaagg tctatgacgt cgtcgtcgag aacgtcagtc
     9421 tggtccagaa ctaccgcacc cagtttaacg gcatcctggc gaaaagcacg ccggaagagt
     9481 tgctggtcac cctgcggaaa aaggtgaaag gaccctgagc acgatgtccg gagagaaccg
     9541 cagcgtcggc agtatgattc ccgctttgcg atcgtgcctg atcgcggcgc tggcgttcgt
     9601 ttttctcgcc gggggctgcg cccacggccc caaacaggcg ccggagtcgc ccgccccggc
     9661 ggcgctgacg cgggttacgg ccgaccccgc cgtggtcacg gccgctccgg ccccggaatc
     9721 gggagcgtct gcccggccca aagacaagga gatcacggcg gcggccccgg cgccacggat
     9781 tgccgccaat tccccaaatg ccgcgccatt acccccggtc gccaaggcga cgaatccggg
     9841 gccggatcct tcctcgtccg aacccggtgc acaggcggac acggaagaaa cctccggcga
     9901 agcggacgag ggcctcgagg gcgatttcat cgccactgac gacgaagggg aagcggactc
     9961 agccgccggc ccccgggagg aggccctcac catcgccgac ccctgggagc ccttcaaccg
    10021 ggccatgtat catttcaacg acaatctcta cttctgggtc ctcaaaccgg tggcccaggg
    10081 atacgccaag gcgcttccgg agggggtgcg gatcagtatc cggaacgtct ttgtcaacat
    10141 cgccttcccg gcgcggttcg tcaattgcgt cctccagttg gatctgccct gcgccgccgt
    10201 ggaaacgggg cggttcgtca tcaattcggt ctggggtctc gggggtctgt tcgacctggc
    10261 ctccgatgag gcaatcaacc tccccaagca gcgccgggat ttgggtcaaa ccctgggcgt
    10321 ccacggcatc ggccccggtt tcttcatcaa ctggcccatc ttcggtccct ccagcccccg
    10381 ggataccgtc gggctgatgg gggacatcct catggaccct ctggcctggg tctccccctg
    10441 gtatgccgcc accggcatcg ccgccggtta caaactcaac gagacatccc tgagcatcgg
    10501 cgactacgaa gccctcaagg aagcggccat cgacccctat gtggcgattc gggatgccta
    10561 tgtgcagtac cggtaccaca agatcaaaca agtgacggac cgcaaatgaa gcggaagccc
    10621 gcccccggca cggacaagcc gccgccggtc tcggacaggc aattccggga gaagcgatga
    10681 aatttttacc ctccgtagtc agctacttcc tcaaagggga gagcacccgg cagaacatca
    10741 ggatgctgct gcgcttcttt ctcgcgctgt tcgtcatgat caccgcctac agcgtcattt
    10801 ttcacctcct catggcggcg gaagggcaga ggcactcctg gatcacaggc ttatactgga
    10861 ccatggtcgt catgagcacc ctgggcttcg gcgacatcac cttccattcc gacgtggggc
    10921 gcatcttctc catcctcgtg cttttatccg gggtaatctt cctgttgatc ctcctgccct
    10981 tcgccttcat caagttcttt ttcgccccgt ggatcgaggc ggaaaccagg aaacgctatg
    11041 cccaggaact gcctccgggg acaaaaaatc acgtcatcat caccgcttac gatcaagtaa
    11101 ccatggccct catcgaaaag ctgggcattt acaaacggga ttatgtggtg atcgtggacg
    11161 aatacgagcg gtcgaaggaa ctctatgaca caggggtccg aatcgccgtg ggccacatcg
    11221 acgatccgga aacctaccgg aagatgcggg tggaacaggc cgccatggtc gtggcgacga
    11281 accgcgacga gatgaacacc aacatcgcct tcaccgtccg ggagacaacg gaaaccgttc
    11341 ccgtggtcgc cacctccgac tcaccccact ccgtcgatat cctctatttg gcgggatgca
    11401 cccgggtcct ggaactgacg gagatcctcg gtcgttccct ggcgggctgg accctcggcg
    11461 gcgattttcg cgccaatgtc ctggggcgtt tcgacaacct catcatcgcc gaagcccccg
    11521 cgatcagcac cccgctggtg ggtaaaaccc tggcggaaag ccgccttcgg gaagagatcg
    11581 gggtaacggt catcgccgta tgggaacggg gcagattcgt ggcgccgacc ccggaggccg
    11641 cgatcaatcg ctccactgcc ctggtcttcg ccggcacgga agagcagatc gcccgttatg
    11701 acgaggtgta cggattctac cagttgaccc gccacgccgg cgacccggtg atcatcatcg
    11761 gcggcggacg ggtgggaaag gcgatcgccc agcggttcaa ggagcgggag gtggattacc
    11821 tcatcgtgga aaagaacccc cggcgcacgg aggacgacac ctgctacatc accggcgacg
    11881 ccgccgatat cggcaccctg aagcgggcct ggatcgaaaa gacccccgcc gccctcatca
    11941 ccacccacga cgacgcgacc aacatctatc tcgccaagta cctccggagc ctccgtccgg
    12001 acatgcagat aatctgccgg gccaacacgg accggaacat ctccaccctg caccgggccg
    12061 gggcggattt cgtcatgtcc tacgcctccc tgggcgccaa cgcgatcttc aactacctcc
    12121 ggaaggagga catgttcatg cttgccgaag gcctgaacat ctttaccatt cctctgcccc
    12181 ccgccctggc gggtaagacc ctggcccagt cccggatccg tcccaaaacg ggctgctccg
    12241 tcatcggcat ccgccggggg gataacgtcg ccatcaaccc cgatcccttc ctccccgtgg
    12301 aggccgattc cgagctgatc ctcatcggct cccccgaagc
//
LOCUS       DAOSMF010000053        12149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_6071_length_12149_cov_18.089797, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000053 DAOSMF010000000
VERSION     DAOSMF010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 12149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12149
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6071_length_12149_cov_18.089797"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="PLT63_06545"
     CDS             <1..363
                     /locus_tag="PLT63_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98418.1"
                     /translation="GDFLAARLEHLKCVESWKQSGDGAAVNAAMAEYSAFVRRDPIFK
                     ALVAVLVRIVQENPGILQSDISKSAESSSWPALYNYNRPLSREDISYALYFAHEFGIV
                     ARTKKGRSYELRLASNSL"
     gene            360..1430
                     /locus_tag="PLT63_06550"
     CDS             360..1430
                     /locus_tag="PLT63_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98419.1"
                     /translation="MKHLLLCPACHHPFGVSHRFCTCCGARLDPLAALERSIRGIGHP
                     EFPFLDPVYSPGSRIPCRDFSGFMAECLQANDSQTVARLFRYLIAGNPGVGVSDDWGA
                     DWVQEVNALPLSQYVQTGNPIVIHEIKALLILAGLGGGPRQFHITDGIPAHLARALSC
                     HHLTWLPAMAELHERVNQFIRHMRREAPFWKEFPLIKTDRLLNFPLPESALRTLLENF
                     SPSVRALALYFADDFRWSIPKSISYGDRRFGIHPEEAAAEIENTDLCEHANAPPGNLL
                     KAWEKDRFLQHCHNLGIETKSSWSKSRLLDELLQRNPELMERSVRRESAVRLNPKYAE
                     DMKRIKDHASFLEDHIKALIFL"
     gene            complement(1506..1826)
                     /locus_tag="PLT63_06555"
     CDS             complement(1506..1826)
                     /locus_tag="PLT63_06555"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HOD98420.1"
                     /translation="MNRGEVYWVNLDPTVGSEIQKKRPCVLLGANPINQARRTVVVVP
                     LSSAGQVRPPLTVAVQCLGRAAVAVCDQIRAVDKSRLLERAGEVSDEDLRAIEDGLRQ
                     VLCL"
     gene            complement(1813..2046)
                     /locus_tag="PLT63_06560"
     CDS             complement(1813..2046)
                     /locus_tag="PLT63_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98421.1"
                     /translation="MTKVLVSMPARVLSRMRALVPDRQRSRFITRLVEAELAKREAEL
                     FQSARAVDQDEALNAEMREWDVTAADGVDHEPW"
     gene            complement(2207..2668)
                     /gene="mobC"
                     /locus_tag="PLT63_06565"
     CDS             complement(2207..2668)
                     /gene="mobC"
                     /locus_tag="PLT63_06565"
                     /inference="COORDINATES: protein motif:HMM:NF017524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HOD98422.1"
                     /translation="MRTQQLLVRLSEAEQAELRRCAAAAGVAVSEYVRGRLLGPAAPA
                     AAAGMEAETWRGGRALLQVRVRPETKAAIRARAERAGLSLSAYVRRAALERDVVHRFD
                     HAAVHQLRRIGVNLNQTVRVMHQGQFSEAVRESLRRCLVALEEQLGKIPTR"
     gene            3562..4755
                     /locus_tag="PLT63_06570"
     CDS             3562..4755
                     /locus_tag="PLT63_06570"
                     /inference="COORDINATES: protein motif:HMM:NF016670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HOD98423.1"
                     /translation="MIRADRLLSQQEMGWLFRPLAICPFPAKPFKGEGPEETPLLWSR
                     KAGNVSVEIIGHPKYGVPYGQDILIVLYLAVEAQRQKSRKITVNFYRDFMRMFDMNPN
                     DGRKYGLVKQSMTRIRNALFKWEVEQGEHREKGATYVYIEEWDLYFDPKNPTQRPLLD
                     QYILLSERFWHEISTHKIPFNLEAVKHLKAKISHLNFYIWLSYRVWRAWQEKEKQGLP
                     SGEAFVPFWGEYGLQGQMSSVIEKKFEFRREVRKWLDSVKELWPKCPVDINGDGLKIQ
                     VAGKDQLDIMPEDTDSPRIFPNVESQSITAFARTDICPKCGKPRTLQPGKINQQKNFR
                     YPDWWSCSGKCSAEALNANCPDCNMPMEVVNKGRQDYHYQCLNCGRIEGGEAYWLKYS
                     VFVQR"
     gene            4823..5122
                     /locus_tag="PLT63_06575"
     CDS             4823..5122
                     /locus_tag="PLT63_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98424.1"
                     /translation="MNKLFPLILRTLFSIWTVSAQRVYAGQSIEEILALPEDQIDTGH
                     ACLVLAKDAFPDLNVAAFDRLLDKIAGRPRACSTRCRRTSPRNWTASGSPSTAMR"
     gene            5092..5511
                     /locus_tag="PLT63_06580"
     CDS             5092..5511
                     /locus_tag="PLT63_06580"
                     /inference="COORDINATES: protein motif:HMM:NF017953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HOD98425.1"
                     /translation="MRIAFDGDAVIFSGEAERIFQEQGLEAFEHHEKENADRPLPEGP
                     FARLLKTLADLQRQNRPDCPIRLALMTARSSPAHERVLKTLRAWQVRIDEMFFLGGVS
                     KDEVLRAFQPHIFFDDRDAHCIPAARVAPTAQVPTDI"
     gene            5745..6155
                     /locus_tag="PLT63_06585"
     CDS             5745..6155
                     /locus_tag="PLT63_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98426.1"
                     /translation="MNYLAYFSIMLVSSILCLTPVTDDEAVRSPIIKEEFVGIRFFID
                     DVEIGSAYGSESTPIKFGLKGKSVTISPIIRDRKKKIVQLIILEDTKGQTKKTLERTK
                     ISPSMPGKITILHDKIIIVKLIDITSTPECSRPR"
     gene            6181..6735
                     /locus_tag="PLT63_06590"
     CDS             6181..6735
                     /locus_tag="PLT63_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98427.1"
                     /translation="MFKNFFVSLMILFWLVGGVKAGDNSPYIITLSFLESNHSKAFSV
                     DVSEDMMATIDISALHEVYGIKVEVLDPETRTICVKFYSVINANYGEAKLVETKVISR
                     RDIYAATENTNAEFVFRAQFSGLTEKAIRGDALLATRCDTCCVTCERIPGWPTTVCGI
                     RAICDTECCTCDLSQPCPVAPPKH"
     gene            complement(7016..7228)
                     /locus_tag="PLT63_06595"
     CDS             complement(7016..7228)
                     /locus_tag="PLT63_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98428.1"
                     /translation="MSYEEDEENAYRTYDDIVGRSIRRGTRKAVKALTFGLLGGTDPL
                     TELGTDAAEELITKVIPRSQENSEDD"
     gene            7399..8163
                     /locus_tag="PLT63_06600"
     CDS             7399..8163
                     /locus_tag="PLT63_06600"
                     /inference="COORDINATES: protein motif:HMM:NF018290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOD98429.1"
                     /translation="MIVSILNQKGGVGKTTASVHLAYAFAVAGRKTLLIDLDPQANAG
                     TTFFEELPKTGTIREPMLDRRYDIRESVYQAHEDGVVVPNLFIIPANIHLEAAERNIY
                     AMPHREKILKNQLKKIERDYDFILVDCPPRLNQLTINAIYAADLLLIPTVLDRYSLDG
                     ISDLFTTIEEVKEGEFPHYLIFKNAYDSREKKSNDLAESILRDLENHICVTAIRRTSL
                     FKQAQQHNQTLFAYDPKSPACEDFINLSKEITSYAL"
     gene            8153..8464
                     /locus_tag="PLT63_06605"
     CDS             8153..8464
                     /locus_tag="PLT63_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98430.1"
                     /translation="MPSKKSGQADPASKFKLRKRTEGILEKEDISEIVGTGVKKAKPI
                     TYTLRYSENESQMLQKLTDEVNAFSTVKTISKNDVIRALIVMGYDLSREKILNALRSL
                     I"
     gene            8679..8951
                     /locus_tag="PLT63_06610"
     CDS             8679..8951
                     /locus_tag="PLT63_06610"
                     /inference="COORDINATES: protein motif:HMM:NF017243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HOD98431.1"
                     /translation="MILLRSVDSDRNRFRQYSLDLQPGLIDGMSLVVTWGRLNARRLR
                     RRVYWFPDAAAARRRARAIYRRRLRHHYRVERIGGWLAQPDVLETT"
     gene            8948..9577
                     /locus_tag="PLT63_06615"
     CDS             8948..9577
                     /locus_tag="PLT63_06615"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HOD98432.1"
                     /translation="MKTIAYLRVSKESQDADNQRLAILDYAHRNKFRVDEFIEIVVSS
                     RRSPQERGLDELLKKLAAGDRLIVSELSRLGRSVGQIIILVHELVRRGVAFTAIKENI
                     ALGQRRYDLQGKVMVTLFGLFAEIERDLISERTKSGLAAARARGQRLGRPPGTFSSRL
                     DSRRPEIEQLLALKVSKASIAKIMGVSRSALVAFIERRGLAAANTRGSR"
     gene            9574..10302
                     /locus_tag="PLT63_06620"
     CDS             9574..10302
                     /locus_tag="PLT63_06620"
                     /inference="COORDINATES: protein motif:HMM:NF013964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HOD98433.1"
                     /translation="MSENPKNPKRRPARSRDIPIRTKRLIIQEAGGVCPFCGEQDADS
                     FDFHHIHGKDGESPHAPDNLIYVCKNCHGRITAGAISEADVHLTKQILKFQGNPFLKQ
                     QAESNVLNFTGGINTGTVANEYHYHNHGKSPKIPRPTPTSGFIGRDLLKRNYLLHLIK
                     RYQEFAKAQKDREYKYYRIYTSIKQQFGVTWDMIPIELFDSACRYVQERIDATALGRN
                     RKAHNQPNYSTFQEYCQKYIEKRK"
     gene            10299..11999
                     /locus_tag="PLT63_06625"
     CDS             10299..11999
                     /locus_tag="PLT63_06625"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOD98434.1"
                     /translation="MNQLYFGDNLTVLRESIPAASVDLVYLDPPFNSKATYNVLFKAP
                     NGHDSDAQITAFEDTWHWGHQAEQEFDELLHQPNTRAAEMVRAFRQFLGENDMMAYLV
                     MMANRFLELHRVLKPTGSLYLHCDPTASHYLKVVLDAIFGSENYVNEISWKRTSAKSD
                     YSQGAVHWPRVRDILFYFQKNSRCGATFFPQFLPHDEEYIRSKYPYLDASGRRYGLWD
                     MTGPGGAAKGNPRYEVMGITKFWRYSREKMEELIRENRVVQPRPGAVPRQKRYLDESA
                     GVAIGDSWDDIPPINSQAQERLGYPTQKPLALLERILRASSNEGDLVLDPFCGCGTAV
                     HAAQKLNRQWIGIDITHLAVSLIEKRMKDAFPGIQFEVHGTPKDAAGARALAGRDKYQ
                     FQWWACSLVNAQPYLGRKKGADSGVDGVLYFLDGPDAARPEERPGEIVAEAQVPYAGA
                     PVTSKKIVVSVKGGEHLHVAMIRDLVGVVEREKAEMGLFVSLVEPTQPMRVEAAKAGF
                     YVSPYFPDRKYPRIQLLTVPGLLEQREHPLYPDFSGGGLTFKQARREAGQRDQKKLFE
                     "
BASE COUNT         2742 a         3486 c         3326 g         2595 t
ORIGIN      
        1 ggggatttct tggcggctcg cctggaacat ctcaaatgcg tcgagtcctg gaaacaatcc
       61 ggcgatgggg ccgcggtgaa tgcggccatg gccgaataca gcgcgttcgt ccggcgcgac
      121 cctattttca aggcgctggt cgcggttctc gttcggattg tccaggaaaa ccccggcatt
      181 ttgcaatccg acatctccaa atctgccgaa tcatcgagct ggcctgccct ctataactac
      241 aaccgcccac tgtcccggga ggacatttcg tacgctctct atttcgccca cgagttcggt
      301 atcgttgcgc ggacaaaaaa ggggcggtct tacgagctgc gcctggcttc caacagtcta
      361 tgaaacatct tcttctctgc cccgcatgcc atcatccctt cggggtgagc caccgttttt
      421 gcacctgctg cggagcgcgg ctggatccac tggccgctct ggaacggagc atccggggga
      481 tcggtcatcc ggaatttccc tttctagatc cggtttattc gccggggtcc cgcatcccct
      541 gccgggactt ttccggcttc atggccgagt gtttacaggc caacgactct cagaccgtgg
      601 ctcgcttgtt ccgttatttg atcgcgggaa atcccggcgt gggcgtttcg gacgattggg
      661 gggcggactg ggtgcaggaa gtgaacgctt tgcccttgtc gcagtatgtc caaaccggaa
      721 acccgatcgt catccatgag atcaaagcgc ttctcatcct ggccggtttg ggcggcggac
      781 cacgacaatt tcatattacc gacggaatcc ccgctcacct cgcccgggcg ctgtcctgcc
      841 accacctcac ctggctgccc gccatggcgg aactacatga aagagtcaat cagttcatcc
      901 gccacatgcg gcgggaagcc cccttctgga aagaatttcc gctcattaaa acagaccgct
      961 tgctcaattt cccgttgccg gagagtgcgc tgcgcacgct gctggaaaac ttctcgcctt
     1021 ccgtccgtgc ccttgcgctt tatttcgctg atgacttccg ctggtccatc cccaagagta
     1081 tctcttacgg ggaccggcgg ttcggcatcc acccggagga agcggccgcg gaaatcgaga
     1141 ataccgatct gtgcgagcat gcaaacgccc cgcccggcaa cctgctgaag gcctgggaaa
     1201 aagaccggtt cctgcagcat tgccacaatt tggggatcga aaccaaatcc tcttggtcca
     1261 aatcccggtt gttggatgaa ctgttgcagc ggaatcccga actcatggaa cggtcggtgc
     1321 gccgggaaag cgcggtccgc ctcaatccca aatacgccga agacatgaaa cggatcaaag
     1381 atcacgccag tttcctggaa gaccacatca aggccctgat tttcttgtga tgcgctttaa
     1441 gcgcgggcgc tgaccagcgg cgcgggggag ggggccgccc ccgcccgacg cgggcgcggg
     1501 ccggtttaca agcagagcac ctgccgcaag ccatcctcga tggcccgcag atcctcgtcg
     1561 ctaacctccc cggcgcgctc cagcagccgg ctcttgtcca ccgcccggat ctggtcgcac
     1621 accgccaccg ccgcccggcc cagacactgc accgccacgg tcaggggcgg ccggacctgc
     1681 cccgccgaag acagcggcac caccaccacc gtccgccggg cctggttgat cgggttggcg
     1741 cccagcagca cgcacgggcg ctttttctga atctcggaac ccaccgtggg atcgaggttg
     1801 acccaataca cctcaccacg gttcatggtc cactccgtcg gccgcggtca cgtcccactc
     1861 gcgcatctcg gcgttcagcg cctcgtcctg gtccaccgcg cgagcggact gaaacaactc
     1921 cgcctcccgc ttcgccagct ccgcctccac caggcgggtg atgaaccggc tgcgctggcg
     1981 gtcgggcacc agcgcccgca tccgggacag cacccgcgcc ggcatggata ccaacacctt
     2041 ggtcatgatc ggcctccttt tcctgttagg atataatata tccacgtgct tggcaagccc
     2101 cgagcgagaa atccaatttt gaaacgggtc cggcggtatc ccgatttccc gctcgtggcg
     2161 gctccgttgc ccgccccgcc gcctcgccgg gagaacgccc ccccggttac cgcgtcggga
     2221 tcttccccag ctgctcttcc agcgccacca ggcaccgccg cagggactcc cgcacggctt
     2281 cgctgaactg gccctgatgc atcacccgaa ctgtctggtt gaggttcacc ccgatccgcc
     2341 gcagctggtg gaccgccgca tggtcgaacc ggtgcaccac gtcccgctcc agcgccgccc
     2401 ggcgcacgta cgccgacagg ctcagccctg cccgttccgc cctggcccga atcgccgcct
     2461 tggtctccgg gcgcacccgc acctggagca gcgcccgccc gccccgccaa gtctccgctt
     2521 ccatcccggc ggccgcggcc ggcgccgccg gtcccaagag gcggccgcgg acgtactccg
     2581 acaccgccac accggcggcg gccgcacacc gacgcagctc cgcctgctcc gcctcgctca
     2641 ggcgcaccag caactgttgc gttctcatgc cggctccttt ctccctctcc gggggtccgg
     2701 gggcaacgcc cctggcgggg tccgggggta tcccccggcg ggcatttgta tatacgcagt
     2761 atagacaaat gcgtatcctg ccctcaagtg atttctctct cggggggcgg ttcagccgat
     2821 cgtgcccgac cgcttgccca gagggttgcg tccgacccca aaagggacac ccgcccggcc
     2881 gccgtttcca ccgttgcgga gacggcccgg cgggtcgatt tcgcaacgcc agcggcgcac
     2941 aggggggtat tccaggatcg gggtgcgtcc agatcgcccg tcctggagcc tcctgtgcgg
     3001 tcaattttcg ggctgtctgg ctccagggag cgtttccccc tgtttcgcct ccgccgtggg
     3061 gaaacgttca gggagggtaa aacagaccgg gggcgagtcg atcgttgctc ctggggcatc
     3121 ctggagggca ggaacgggag cgttcacgtc ggtcacggtg ggttttttag cggtgcggag
     3181 agagaggaac taaacatctc gtcggcgggt gcggaatgtg ggaaacccgt ctggcgggtt
     3241 ttccacattt ccacccgcct acttgataca tatcctattt ggtaaataca ttctatttga
     3301 taagcatgaa taaattcaca tggaaaacag gaccttttcg aagtcctgca tgaatagatt
     3361 cacatagccc gcatgaatgg attcacatgc gaattgtgga aaattcctat tcatgactgt
     3421 ctccatgaac atgtatagat tcacatgtct aacatgttga aaaaattgag ttgaaaaacg
     3481 aacggagaat cattaatatt tcaattcatg aaagaagacg ccaaaagaga taaacgggag
     3541 aatcaaacag ccgccgtggc catgattcgg gcggatcgat tgcttagcca gcaagaaatg
     3601 ggatggctct ttcgtcccct ggccatttgt ccgtttccgg cgaagccgtt caagggggag
     3661 ggacctgagg aaacaccgct tctctggagc agaaaagccg gcaacgtatc cgtagagatc
     3721 atcgggcacc ccaagtatgg cgttccctat ggccaggata ttttaatcgt gctctatttg
     3781 gccgtagaag ctcagcgaca gaaatccaga aaaatcaccg tcaattttta tcgtgacttc
     3841 atgcgcatgt ttgacatgaa ccccaacgac gggaggaagt atgggcttgt aaagcagagc
     3901 atgaccagga ttcggaatgc cctgtttaaa tgggaagtgg aacaagggga acaccgggaa
     3961 aagggagcga cctacgttta cattgaggaa tgggatcttt acttcgatcc gaaaaacccg
     4021 acccagcggc cgctgttgga tcagtacatc ctcctctctg agcgtttctg gcacgaaatc
     4081 agcacacaca agatcccgtt caatctggaa gcggttaaac atttaaaggc gaaaatttcc
     4141 catttgaact tctatatttg gctttcgtac cgcgtgtgga gggcctggca ggaaaaggag
     4201 aagcagggat tgccttcggg ggaagccttt gtcccgtttt ggggagagta tgggctccaa
     4261 ggccagatgt cgtccgtgat cgaaaagaaa tttgaatttc gccgggaagt gagaaaatgg
     4321 ttagattccg tcaaggagct gtggcccaaa tgtcccgtcg atatcaacgg tgatggatta
     4381 aaaattcagg tggccggcaa ggatcaactg gacataatgc cggaggatac cgattctccg
     4441 aggatttttc caaacgttga gagccaatca ataacggctt ttgcccgaac ggacatctgt
     4501 cccaaatgtg ggaaaccgag aacgcttcag ccaggaaaga ttaaccaaca aaagaatttt
     4561 cgctatccag actggtggag ttgttccggc aaatgctccg cggaagcgct taatgcgaac
     4621 tgccccgatt gcaacatgcc catggaagtg gttaacaaag gtcggcagga ctaccattat
     4681 caatgcctca actgtggccg aattgaagga ggagaagctt actggctgaa atacagcgtg
     4741 tttgttcagc ggtgaatcat gctcccaaat tagtgagcaa cgatggtaaa atattgccga
     4801 ctttcctctg ttgggtggat tgttgaacaa gctgtttcca ctgatcctcc ggactctctt
     4861 ttcaatctgg acagtctcgg cccagcgggt atacgcgggc caaagcatcg aggagattct
     4921 ggccctgccc gaggaccaga tcgacaccgg ccacgcctgc ctggttctgg cgaaggacgc
     4981 ctttcccgat ctgaacgtcg ccgccttcga tcgcctgctg gacaaaatag ctggacggcc
     5041 gcgggcctgc tctacgcgct gccggcggac ttcaccgcgg aactggacgg catccggatc
     5101 gccttcgacg gcgatgcggt gatcttttcc ggcgaggcgg agcggatttt tcaagagcag
     5161 gggctggagg ccttcgagca ccacgagaag gagaatgccg accggccgct gccggagggc
     5221 ccgttcgccc ggcttctcaa gacccttgcc gacttgcagc ggcagaaccg gccggactgc
     5281 cccatccggc tggctctgat gacggcccgg agcagccccg cccacgaacg ggtgctcaag
     5341 acgctgcgcg cctggcaggt gcggatcgac gaaatgttct tcctgggtgg cgtgtccaag
     5401 gacgaggtac tgcgggcgtt tcagccccat atcttctttg acgaccgcga cgcccactgc
     5461 atcccggccg cccgggtggc ccccactgcc caggtcccga ccgacatctg acatgtaccg
     5521 cggcaggggc acaaaggcga tataagttgg aacgccagaa acaaggttca gcttggtgga
     5581 aattgccgtc tgtggctttt accagactgc acgaacttgc tgccgcgagt gtaagtaacg
     5641 tacatattca agaaaattct gttgacatac agaggtttta ctgtctaatt attgaagcat
     5701 gaaaaagagt agaattacaa agaagtaatc aaagaggtat tgctgtgaat tatctcgcct
     5761 acttttctat tatgcttgtg tcatccattc tttgtctaac tccggttacg gatgatgagg
     5821 ctgtacgctc tccaattatc aaagaagaat ttgtaggcat cagatttttt attgatgatg
     5881 ttgaaattgg atctgcatat ggatctgaat ccactccgat aaaatttgga ctgaaaggga
     5941 aatcggttac tatctctccg atcatcagag atcgcaagaa aaagattgtt caattgatta
     6001 ttttggaaga tacaaagggt caaaccaaga agacacttga acgaacaaag atatctccgt
     6061 caatgcctgg taaaatcaca atactacacg acaagataat aattgttaaa ttgatcgata
     6121 taacaagcac gcctgaatgc tctagaccaa gatagccaca ttctattgcc aggaggcggt
     6181 atgttcaaaa atttctttgt ttcattgatg attctcttct ggttggttgg tggtgtgaag
     6241 gcgggtgata actcgccgta cattattacc ctcagttttt tagaatctaa tcattcaaag
     6301 gcgttttctg ttgatgtatc ggaggatatg atggctacga tcgacatttc agcattgcat
     6361 gaagtgtatg gaataaaagt tgaggtctta gatccagaga cgcggacaat ttgtgtcaaa
     6421 ttctactccg ttatcaacgc aaattatggc gaggcgaaac tagttgaaac aaaagtaatt
     6481 tcccgtcgtg atatttatgc tgctacagag aacaccaatg cggaattcgt atttcgtgcg
     6541 caatttagtg gattgacaga gaaagcgatt cgtggtgatg cactcttggc aactcgttgc
     6601 gacacatgtt gtgttacttg tgagcggatc ccgggttggc caaccacagt atgtggcatt
     6661 cgagcaatat gcgacaccga atgttgcaca tgtgaccttt ctcaaccgtg tcccgtagct
     6721 ccaccaaaac actgaaattt tattggtatc aaattaaagg tcaatcatcc ccatcgttta
     6781 ggggggatga ttgacctttc gcttctgatt attgagagga taaatagcag cggtgggtca
     6841 tgccggccgt cttcgacatg cctggatgta gagaccatcg aatcctccgg gagcttatcc
     6901 cggattgggc ggcagccgtt tcagcagacg gttcctgcct gactgcaaaa ccgaccctct
     6961 ataatacccc aagacggtcg attggagttt gaccctggcc cggagtaccc gctgttcagt
     7021 cgtcttcact gttctcctgg gaacggggga tcactttcgt gattagctct tccgccgcgt
     7081 ccgtgcccag ttcggtcagc gggtctgttc cacccaacaa accgaaggtc agggccttta
     7141 cggctttgcg agttcccctc cggatggacc gccccacgat gtcgtcatag gtgcggtagg
     7201 cgttttcctc gtcttcttcg tagctcatta caacctcctt ttttccatta taccaaaagc
     7261 aattgtttac ttggctattc gtgcgaaatc cgctattggt aaagtttttg gcgaaacttt
     7321 tataaatttt cggcattatc attgacttct catgtgactt caattatggt ttcatatatg
     7381 acttcttaag gagctgccat gatcgtttca attctgaacc aaaagggcgg cgtcgggaaa
     7441 acaacggctt ccgttcacct ggcttacgcg tttgccgtcg ccggccggaa gaccttgctg
     7501 atcgacttgg acccgcaggc caacgcggga accactttct tcgaggagct gccgaagacg
     7561 ggcacgattc gtgaacctat gcttgatcgc cgttacgaca ttcgggaatc ggtttaccag
     7621 gcccacgagg acggcgtagt ggtccccaat cttttcatca tccccgcaaa catccacctg
     7681 gaggcagcgg agcgaaacat ctacgccatg ccgcaccggg agaaaattct gaaaaaccag
     7741 cttaaaaaaa tcgaaagaga ctacgacttc atcttggtcg actgcccacc caggttgaac
     7801 cagttgacca tcaacgccat atacgcggcc gacctactcc ttatccccac cgtcctagac
     7861 cgctactcgt tggacggcat ctccgacctg ttcaccacca tcgaggaggt caaggagggt
     7921 gaatttcccc attacctgat cttcaagaac gcctacgaca gccgggagaa aaaatccaac
     7981 gacctggccg aatcgattct gagagatctg gaaaaccata tctgcgtaac ggcgatccgt
     8041 cgcacgtccc tgttcaagca agctcagcaa cacaaccaaa ctctttttgc gtacgatccg
     8101 aaatcgcccg catgcgagga cttcataaac ctatcaaagg agattaccag ctatgccctc
     8161 taaaaaatct ggtcaggctg atcccgccag caagttcaaa ctccgcaagc ggactgaggg
     8221 aattctggag aaagaggata tctcggaaat cgttgggacc ggcgtcaaga aggccaaacc
     8281 catcacctac actcttcgtt actcagaaaa cgaaagtcag atgctgcaaa aattgacgga
     8341 tgaagtgaac gcattttcaa ccgttaaaac aataagtaaa aatgatgtaa taagggcctt
     8401 gatcgttatg ggatatgacc tatccagaga gaagatattg aatgcattga gaagtcttat
     8461 atgacttcac aaacggtttt attcttgaat tcatattgtg tgtcattctt gaagcgggac
     8521 actcactgga aaaacttctc ccggttctga gaattttgcc ggtcccggtg gtgattattt
     8581 aaacgaccgt tattctggtg agtatttagt cttctatagg gtgtcttaaa actgcgggaa
     8641 atgatatcat taactctgat ttataggttt tccgcatcat gatcctactt cgctccgtcg
     8701 attcagatcg caaccggttc cgccaataca gcctggatct ccaacccggc ttgatcgacg
     8761 gcatgagcct ggtggtcacc tggggccggc tgaacgcccg ccgcctgcgc cggagggtgt
     8821 actggttccc agatgccgcc gccgcccggc gccgggcacg ggcgatttac cgccgccggc
     8881 tccgtcacca ctaccgggtg gagcggatcg gcgggtggct cgcccagcca gatgttctgg
     8941 agacgacgtg aaaaccatcg cctacctgcg ggtgtccaag gaaagccagg acgcggacaa
     9001 ccagcgcctg gccatcctgg actacgccca ccggaacaag ttccgggtgg acgagttcat
     9061 cgagatcgtc gtctcctccc gccggtcccc gcaggaacgc gggctcgacg agctgctgaa
     9121 gaagctggcc gccggcgacc ggctgattgt cagcgagctg tcccgcctgg gccgctcggt
     9181 ggggcagatc atcatcctgg tccatgaatt ggtccggcgg ggagtggcct tcaccgccat
     9241 caaggaaaac atcgccctgg gccagcgccg ctacgacctg cagggcaagg tgatggtgac
     9301 cctgttcggc ctgttcgcgg agatcgagcg ggacctgatc tcggaacgga ccaaatccgg
     9361 gctggcggcg gcccgggccc ggggccagcg cctgggccga ccgccgggca ccttcagttc
     9421 acgcctggac agccgccgtc cggagatcga gcagctgctc gcgttgaagg tgtcgaaggc
     9481 cagcatcgcc aagatcatgg gcgtgtcccg gtccgccctg gtggccttca tcgagcggcg
     9541 gggactggcg gcagccaata cgcgagggtc gcgatgagcg aaaatcctaa aaatcccaag
     9601 aggagacccg cgagatcccg tgacattccg ataaggacga agcggctgat catccaggaa
     9661 gcgggcgggg tgtgcccttt ttgtggtgag caggatgccg attccttcga ttttcatcac
     9721 atccacggaa aggacggcga gtctccccac gccccggata atctgattta cgtgtgcaag
     9781 aattgccatg gccgaatcac cgccggagcc atctcggaag cggacgtcca tctcacgaaa
     9841 caaatcttga aattccaggg aaaccctttc ctgaagcaac aagcagagtc gaacgtcttg
     9901 aatttcacgg gaggaatcaa tactggcacc gtggccaatg aataccacta tcacaaccat
     9961 gggaagtcgc ccaagattcc ccgacctact ccaacgagcg gttttatcgg ccgtgattta
    10021 ctcaaacgaa actacttact gcatttaatc aagcgctatc aagaattcgc caaagcgcag
    10081 aaggaccggg aatataagta ctatcgtatc tacacttcaa tcaaacaaca attcggagtc
    10141 acgtgggata tgattcccat cgaactcttt gactcggcct gccgatatgt gcaagaacga
    10201 attgacgcaa ccgccctggg gcgaaatcgc aaggcccaca atcagccgaa ctacagcacc
    10261 tttcaggaat attgccaaaa atacatcgag aagcggaaat gaaccagctc tactttggtg
    10321 acaatctcac ggtcctgcgg gaaagcatcc cggccgcgag cgtggatctc gtttacctgg
    10381 acccgccgtt caattccaag gccacctaca acgtgctatt caaagctccg aacggccatg
    10441 actcggacgc ccagattacc gcttttgagg acacttggca ctgggggcac caggcggagc
    10501 aggaattcga cgagctgctg catcagccta acacccgagc ggcggaaatg gtgcgcgcct
    10561 tccgccagtt cctgggcgaa aatgacatga tggcctacct ggtgatgatg gccaaccggt
    10621 tcctggaatt gcaccgcgtt ctgaagccca caggcagcct ctacctgcat tgcgacccca
    10681 cggctagcca ctatttgaag gttgtattag atgccatctt tggatcggaa aattatgtca
    10741 acgaaattag ctggaaacgg accagcgcga agagcgatta ctctcaaggc gcggttcatt
    10801 ggccgcgggt gcgcgacatc cttttctact tccagaaaaa ctcgcgctgc ggggccacat
    10861 tctttccgca gttcctgccg catgacgaag aatacatccg gagcaagtac ccctatctcg
    10921 atgcctccgg ccggcggtac ggcttatggg acatgacggg acctggcgga gcggccaagg
    10981 gcaatccccg gtatgaggtt atgggtatca ccaagttctg gcgttattcc agggaaaaga
    11041 tggaggaatt gattcgggaa aaccgggtcg tgcagcctcg acccggagcg gtcccgagac
    11101 agaaacgcta cctggacgag tcggccggcg ttgcaatcgg ggattcgtgg gacgacatcc
    11161 cgccgatcaa ttctcaggct caagagcgcc tgggttaccc cacacaaaag ccgctggccc
    11221 tgctggagcg aattctccgg gcgtccagca acgagggaga cttggtgctc gatcccttct
    11281 gcggctgcgg taccgccgtc catgcggcac aaaaactaaa ccggcagtgg atcgggatcg
    11341 acattaccca tctggcggtg tccctgatcg agaaacggat gaaggatgcc ttccccggca
    11401 tccaattcga ggtccacggc acgccgaagg atgcggccgg cgcccgggcg ctggccgggc
    11461 gggacaagta ccagttccag tggtgggcct gctccctggt caacgcccag ccgtacctgg
    11521 gccggaagaa gggggccgat tccggggtgg atggggtgct ctatttcctc gacgggccgg
    11581 acgccgcccg gcccgaggag cgacccggtg aaattgtggc cgaagcccag gtcccctatg
    11641 ccggcgcgcc ggttacctcc aagaaaatcg tggtgtcggt caagggcgga gagcatctcc
    11701 acgtcgccat gatccgcgac ctagtgggtg tcgtggagcg cgaaaaggcg gagatgggcc
    11761 tgtttgtttc cctcgtcgaa cccacccagc cgatgcgggt ggaagccgcc aaggcgggct
    11821 tctacgtctc cccctatttc cccgaccgga agtatccccg gattcagctc ctcaccgtcc
    11881 ccggcttgct ggagcagcgg gagcatcccc tctacccgga cttttccggc gggggattga
    11941 cctttaagca ggcccgacgg gaagcgggtc agcgggatca aaagaaactc ttcgagtgag
    12001 tcgagacaga gaaaaattca atgcccggga gttccacatg agaaacgaag acgcccagct
    12061 tcatgcccta tgggccaggc aagccgaaca gcggggggat ttcttggcgg ctcgcctgga
    12121 acatctcaaa tgcgtcgagt cctggaaac
//
LOCUS       DAOSMF010000054        12084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_6104_length_12084_cov_22.653587, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000054 DAOSMF010000000
VERSION     DAOSMF010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 12084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12084
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6104_length_12084_cov_22.653587"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(391..466)
                     /locus_tag="PLT63_06630"
     tRNA            complement(391..466)
                     /locus_tag="PLT63_06630"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(431..433),aa:Asn,seq:gtt)
     gene            complement(542..1249)
                     /locus_tag="PLT63_06635"
     CDS             complement(542..1249)
                     /locus_tag="PLT63_06635"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOD98435.1"
                     /translation="MAKMIINGHEIQAKAGATVLEEARKLKIEIPTLCYHKDLSSFGA
                     CRLCTVEIKVKGKWQLAASCQTEAAEGLEVRTDTGRIRESRKLAAELLYSRYPETAAV
                     REIAAKLGVDVRDAAAESHDCILCGLCTRTCHEIVGANALNFRDRGLGREIETPKIEF
                     NSAACIGCGSCAFVCPTGFVRMESVDGKRIIWDKVFKMAACEVCGRYFAPVEQLEYIS
                     RTTGVPVSSLMTCVSCR"
     gene            complement(1291..3216)
                     /locus_tag="PLT63_06640"
     CDS             complement(1291..3216)
                     /locus_tag="PLT63_06640"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOD98436.1"
                     /translation="MNKVAFSSWNGKIIDNRTPAKGKGKAQEPAAGAFPAALEGYSYD
                     ALMGWNGLVVFDPKADVLALTLAYIAEARKLSCGECSVCMIGIDRVREILLGMSAGKG
                     AAKDLAEIEEIVKGVMANSKCNFGRATALTPVLDAVKYFKSAFLEAGKGEKKAAGEYR
                     SAVTAPCMEACPATLDIPGYIELIRNNKFKESLDLIRERCILPGVIGRACTHPCEAAC
                     VRKDMDGPLAIRLLKRAAADAELRGGSALPAPREEKKEKIAIIGAGPAGLAAAYNLRL
                     MGYGVTIFEALPKAGGMASVGIPDYRLPKDILNHEIDLIKRMGVDIRFNSKVEALNMA
                     DFQKQGYAAVFTAIGAHVGAKIGCKGEEIVSEDVVQGAEFLRGLSLGKKVVPVNKVAI
                     IGGGNVALDCARNCVRLGFKNVEIVYRRSRAEMPGSKEEIEEALHEGVKITYLTAPIG
                     IVRKAGKVTAIECVKMKLGDPDESGRKRPIPIKGSEFTLKTDMVIAATGQKPDLGLLP
                     AKEKGSLTTAWGTIKADPASSSTEATRIFAGGDCVTGPATLIEALNAGNKAAKSIAAY
                     LQGETFVDELSFEGIDVSQQREMGFIPPAPAGEVGLLDAAERIKSFAEVEGGFDADAA
                     MKEAQRCLRCYRLMVWQ"
     gene            complement(3278..4231)
                     /gene="porB"
                     /locus_tag="PLT63_06645"
     CDS             complement(3278..4231)
                     /gene="porB"
                     /locus_tag="PLT63_06645"
                     /EC_number="1.2.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019164 - pyruvate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate synthase subunit PorB"
                     /protein_id="HOD98437.1"
                     /translation="MMNTVENFEIYAPKLIEKREFFSPGHRACQGCGEVLALRLLLKA
                     LGEDTVVSNATGCMEIISSAYPHTAWNLPYIHVAFENAAAVASGVEAGLKALRRKGKI
                     ADRYVKSVAIAGDGGTVDIGLQALSGAMERGHDMIFFCLDNEAYMNTGIQRSSATPFM
                     AATTTSPAGKAIKGQRTGKKNVPEIMVAHNVPYVATACPSYPLDLMSKVEKARNTEGP
                     SYIHIFSVCPTGWRAASNETVKLGRMAVETGFFPLYEVENGKYRLTIDYPEKRRPMAD
                     YLKLQGRFRHLDPADIAKVQALVDKEFRKLMHKVKTSVPWS"
     gene            complement(4228..5391)
                     /locus_tag="PLT63_06650"
     CDS             complement(4228..5391)
                     /locus_tag="PLT63_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOD98438.1"
                     /translation="MGKVVGMEVGIAAAEAVGLCNIDVAAVYPITPQSHIAEHLSDIV
                     HDGRIDAEFITVESEHSSISACAGASATGARAYTATSSQGLMLMHEILPIVSAMRLPV
                     VMGNANRAVSGPLNIWNDHTDIMPQRDSGWIVMHAENGQEVIDMTIQSFKIAEHPDIM
                     LPTVLNMDGFQLTHMIEPLEFPSQDEVNAFLPDRVPFATLHPDNPLSMGCFNMPEIFA
                     ETMKAKDAALINSKKTIIKIWNEWGKMFGRSYKPVETYKNKGAETLILTMGSMGETAS
                     VAVDELRAAGQSIGLVKIRLWRPFPLDELRAAIKGCETLIVMDRAISFGGAGGPLGTE
                     IKSLMFHDHDRPRIVDFIMGLGGRDVKVEDFIAMVERAAKKKDETYEYYGVRE"
     gene            complement(5394..5687)
                     /locus_tag="PLT63_06655"
     CDS             complement(5394..5687)
                     /locus_tag="PLT63_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOD98439.1"
                     /translation="MKQKKWPEAWHEVTPGCIVFEPGNASTYKTGSWRAQRPIWDNKK
                     CIKCGICYVFCPEACISETAEGFFAADLDYCKGCGICAHECWPQAIVMVEEEV"
     gene            complement(5709..6257)
                     /locus_tag="PLT63_06660"
     CDS             complement(5709..6257)
                     /locus_tag="PLT63_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase subunit
                     gamma"
                     /protein_id="HOD98440.1"
                     /translation="MIEIRWHGRGGQGAVTSVELLALAAIEEGKYAQGFPAFGPERRG
                     APVMAYNRVSDKPIKIRSGIYHPDVVVVLDPSLVGLVNVTDGLKPDGLLIINTSKPVP
                     TIREQLKYKGKLATVDATHIAREELGVPIANTTMLGAVMKATKVLKLASLDEPIQERF
                     GKIADKNKNALKRAYDDVKLAK"
     gene            6435..>6946
                     /locus_tag="PLT63_06665"
     CDS             6435..>6946
                     /locus_tag="PLT63_06665"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOD98441.1"
                     /translation="MTKAAEMIRKALAEGRQSLSEYDAKTFLGLFGVPVSKDILAADG
                     RAAAAAAESLGYPVVLKAVGNRLTHKTEVGGVVLHVKTAAEVIAESARLMRIPGCETL
                     MVSPLVGGARELVCGLTRDPHFGPAVMFGLGGILTEVLNDTVFRIAPLTEADAEAMLS
                     GIRAKKILDP"
     assembly_gap    6947..7046
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7047..7267
                     /locus_tag="PLT63_06670"
     CDS             <7047..7267
                     /locus_tag="PLT63_06670"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOD98442.1"
                     /translation="DAEAMLSGIRAKKILDPFRGEAAANRQALAKILRAIGRIGVEHE
                     EVAEIDVNPLKIGPDGLPVAVDALVVLK"
     gene            complement(7259..7861)
                     /locus_tag="PLT63_06675"
     CDS             complement(7259..7861)
                     /locus_tag="PLT63_06675"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOD98443.1"
                     /translation="MIRPERTIEKLKRGEKVMIAALGDSLTQGWMVRRGYVDFFRELL
                     PARYPQAVLTMIRKGIPGDTADNGLYRLRYDILEYNPDCIIVQYAINDAFMGYTAGQF
                     RRTIGEIIDEIQADGDADIILTTSSYIGDNADARIIGEFYDQLAELGEKFRLPVAKVH
                     EYWKRCIDAGTPYTTLVQYDMVHPTEDGYRLMAEAILELF"
     gene            complement(7876..8424)
                     /locus_tag="PLT63_06680"
     CDS             complement(7876..8424)
                     /locus_tag="PLT63_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006785305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HOD98444.1"
                     /translation="MTEAHSLKTPLTDADCAALKAGDMVLLSGVVFTARDAAHGRLRE
                     ALRRGEEMPFDLNGAVIFYAGPTPAPPGRVIGAIGPTTSYRMDPFAPLLMERGLKGMI
                     GKGKRSQEVIAAMVRHRAVYFGAIGGVAALTSRCVRNAAVIAYEDLGPEAILRLELAE
                     MPVVVVNDTWGGDLYERARKDR"
     gene            complement(8428..9279)
                     /locus_tag="PLT63_06685"
     CDS             complement(8428..9279)
                     /locus_tag="PLT63_06685"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOD98445.1"
                     /translation="MNPVRNIEAPRITAKVREMFLRANTCLGGDVEDALRRARDGEET
                     ELGRQVLERILENAAVARNERLPICQDTGLAVVFVELGQDVRVVGGALREAVEEGVRQ
                     AYREGYFRKSVCDPLSRANTGDNTPAVIHLDVVPGDAIRLTAMPKGGGSENMSTVTML
                     LPAAGRDGIRDHVVETVLRAGPNPCPPVIVGVGIGGAMEEAVLLAKKSLLRPLGETNR
                     HDGRLALLEAEILAEINRRGGGPQGFGGNASALAVHVEMKPCHIACLPVAVNVQCHAA
                     RHREAEI"
     gene            complement(9293..9892)
                     /gene="recR"
                     /locus_tag="PLT63_06690"
     CDS             complement(9293..9892)
                     /gene="recR"
                     /locus_tag="PLT63_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOD98446.1"
                     /translation="MSRYAPPIERLITELARFPGIGEKTAARLANFILHESLEDARRL
                     AASIMEVKERIRLCRLCFNLTEEELCTVCADPARERGTVCVVQDPDALIAIETTGEYH
                     GTYHVLHGVLAPLDGIGPDDLHLRELLARVDAHSVREVIIATNPDVQGEATALLINKL
                     LAGRPVAISRIAMGVPMGGDLKYTDRMTLGKALEFRRGM"
     gene            complement(9905..>10137)
                     /locus_tag="PLT63_06695"
     CDS             complement(9905..>10137)
                     /locus_tag="PLT63_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOD98447.1"
                     /translation="KTVEVSSGGGMVSVVVNGKFEVMSLKIEKDVVNPDDVEMLQDLV
                     MAAVNEGVRKAQEMNAAEMAKITGGLKIPGLS"
     assembly_gap    10138..10237
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10238..11880)
                     /gene="dnaX"
                     /locus_tag="PLT63_06700"
     CDS             complement(<10238..11880)
                     /gene="dnaX"
                     /locus_tag="PLT63_06700"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOD98448.1"
                     /translation="MDSPAKEYQVMARKWRPQGFEEVAGQDHVVRTLQNAVKLDRIAH
                     AFIFSGPRGVGKTSIARILAKALNCERGPTPTPCQVCIHCREITDGIALDVHEIDGAS
                     NRGIDEVRELRENIKFLPASARFKVYIIDEVHMLTREAFNALLKTLEEPPPHIVFIFA
                     TTETHKIPATILSRCQCFDFRRISRKEIAVNLRKIATGEGIEISDRGVAWIAAAGDGS
                     MRDAQSVFDQVVSYAGETVADEHIEELLGLTDRRFVVAVAAAVLARDGAQCLKLIDEA
                     YYAGMDMPVFYQLLLEHFRNLVLAKVVPEAEGMTDMSREDWSRLQEQTALVSRETLQR
                     LLEILMAEEETMRRTQNPRLVMEMALTRMARLTPLIPIDEILARMTTLESRLRSGMPA
                     PAGETEAGSEDPEAKAVKEPPVSPYGKTAPAPPHPLEEGGGWEGFKDFIRRSHPSLCS
                     KLAPGQLLAREGDVLRIGFPRHNVFFEDIEKHQKRTIAELARKFFQRPDLEVVIEALR
                     GEAAGVNGKPANGPGNFPMNSEIRQEALRNPLLQKVMDV"
     gene            complement(11904..12002)
                     /gene="ffs"
                     /locus_tag="PLT63_06705"
     ncRNA           complement(11904..12002)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLT63_06705"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
BASE COUNT         2182 a         3710 c         3507 g         2485 t
ORIGIN      
        1 taaaatgcgt ttttaagggt caagcccatt gcacaaagaa acaaaggggt ccttgtgcgg
       61 atggagtgac cccttgccct cagatcaatc tcacttccta tgtcagcaac gtattgttaa
      121 cataggacat ttaacgatat tcctttcaat aagctccacg agattggggc aatgatcagg
      181 gctgtttaaa acatcacata tgatacactg aaataaagta acagtaattt tttatttaat
      241 aatttcaaac cgggggggaa aaaaattact ttgaaaaatt tgcatcaaga aatgatgcat
      301 tacgagggaa aatgggtaaa ggatgggtaa aggatgccgc acataagcaa aagggggcta
      361 accatcatct ggctaacccc ctttatttat tggctcctca gcgcggactc gaaccacgga
      421 cccgctggtt aacagccagc tgctctaccg actgagctac tgaggaacgt gtcggtgtta
      481 aaaacgagac cgccttttaa tgcagggcgg tcttcttgtc aatgtttttt tgacttgcgg
      541 gctaccggca gctcacgcag gtcatcaaac tgctgaccgg cacgccggtg gtccggctga
      601 tgtattcgag ttgctccacc ggagcgaaat accggccgca gacttcacag gccgccatct
      661 tgaacacctt gtcccagatg atccgtttgc cgtccacgga ctccatgcgg acgaagcccg
      721 tggggcagac aaaggcgcag gaaccgcaac cgatgcaggc ggcggagttg aattcgatct
      781 ttggcgtttc gatctcccga cccagccccc ggtccctgaa attcagggcg ttggcgccga
      841 cgatttcatg acaggtccgg gtgcacagac cgcagaggat gcagtcatgg ctttccgccg
      901 ccgcgtcccg gacatccacg cccagtttcg ccgcgatctc ccggaccgcc gcagtttcag
      961 gataacggga gtaaagaagc tcggcggcca gcttccggct ctcccggatc ctgcccgtat
     1021 ccgtccgcac ttccagaccc tccgccgcct ccgtctgaca cgaggccgcc aactgccatt
     1081 tgcctttgac cttgatctcc accgtgcaga gccggcaggc tccgaaggag ctgagatcct
     1141 tgtgataaca aagagtcggg atctcgatct tcagctttct cgcttcctcg aggaccgtag
     1201 cgcccgcctt tgcctgtatt tcatgcccat tgatgatcat cttcgccatg attattcctc
     1261 tatcctgtga aattgttccc tgttagctat ttattgccac accatgaggc gatagcacct
     1321 caggcagcgc tgggcctctt tcatggcggc gtcggcgtca aagccgccct cgacctcggc
     1381 aaaactcttg atacgttccg ccgcgtccag aagccccacc tcacctgccg gggccggggg
     1441 gataaagccc atttcccgct gctgcgagac gtcgatgcct tcgaaagaga gttcatccac
     1501 gaaggtctcc ccctggaggt aagcggcgat gctcttggcc gccttgttgc cggcattcag
     1561 ggcctcgatg agtgttgccg gcccggtgac gcagtcgccg ccggcaaata ttctcgtcgc
     1621 ctcggtggaa ctgctggcgg gatccgcctt gatggttccc caggccgtgg tgagcgatcc
     1681 cttttccttg gcgggcagga gccccagatc gggcttctgg cccgtggcgg cgatcaccat
     1741 gtccgtcttc agggtaaact cggaaccctt gatgggaatg ggacgcttgc ggccgctttc
     1801 atcgggatca cccagtttca tcttcacgca ctcgatagcc gtcaccttgc ccgccttgcg
     1861 gactatacct atgggggcag tgaggtaggt tattttcacg ccctcgtgaa gggcttcctc
     1921 gatctcttcc ttggaaccgg gcatttcagc ccgggaacgg cgataaacga tctccacgtt
     1981 cttgaatccc aggcggacgc agttccgggc gcagtccagg gccacgttgc cgccgccgat
     2041 gatcgccacc ttgttaaccg gaaccacctt cttccccagg ctcaggcccc ggagaaactc
     2101 cgctccctgc accacgtcct cggagacgat ctcttctccc ttgcagccga tcttggcacc
     2161 cacatgggcg ccgatggcgg tgaataccgc cgcatagccc tgtttctgga aatcggccat
     2221 attcagggcc tccaccttgc tgttgaagcg gatgtcaacg cccatgcgct tgatcaggtc
     2281 gatctcgtga ttgagaatgt ccttggggag gcggtaatcc ggaatgccga ccgacgccat
     2341 gccgcccgcc ttgggtaggg cctcgaaaat ggtcacgccg tagcccatga ggcggaggtt
     2401 ataggccgcc gccaatcccg cagggccggc gccgataatg gcgatcttct ctttcttttc
     2461 ttcccgcggg gcgggcaggg cggaacctcc ccgcagctcc gcatcggccg cggcccgctt
     2521 caggagccgg atggccaggg gaccgtccat gtccttgcgt acgcaggccg cctcgcaggg
     2581 atgggtgcag gcccggccga tgacgccggg gaggatgcat cgctccctga tgaggtccag
     2641 agattccttg aatttattgt tacggatcag ttcgatgtag cctggaatat ccagcgtggc
     2701 cggacaggcc tccatgcagg gggccgtcac ggcgctgcgg tattccccgg cggccttttt
     2761 ctcccctttc ccggcctcga ggaaggcgga tttgaagtac ttgacggcgt ccagaaccgg
     2821 ggtcagggcc gtcgcgcggc cgaaattgca tttgctgttg gccatgacgc ccttgacgat
     2881 ctcctcgatc tcggcaaggt cctttgccgc gccttttccc gcggacatgc ccagcaatat
     2941 ctcccgaacc cggtcgatgc cgatcatgca tacggaacac tccccgcagg aaagctttct
     3001 ggcctcggcg atgtaggcca gagtaagggc aagcacatcc gccttggggt caaagaccac
     3061 caggccgttc caccccatga gggcgtcgta gctgtacccc tccaaggccg cggggaacgc
     3121 ccctgccgcc ggctcctgcg cctttccctt tcctttggcg ggggtcctgt tatcgatgat
     3181 cttcccgttc caactgctaa aagccacttt attcatgctt ccgtcacctt tatcttgatt
     3241 tcatgaactg tgaatgagtc ttatcctccc cgaggcgtca actccacggg accgatgtct
     3301 tgaccttgtg catcagtttc cggaattcct tgtccaccaa ggcctgaacc ttggcgatat
     3361 ccgccggatc cagatgacgg aaccgaccct ggagttttag gtagtccgcc atgggacgcc
     3421 gcttttcggg gtagtcgatg gtgagacggt acttgccgtt ttccacctcg tagaggggga
     3481 agaaaccggt ttccaccgcc atccggccca gtttgacggt ctcgttagag gccgcccgcc
     3541 agcccgtggg gcatacggaa aagatgtgga tatacgacgg cccctcggta ttgcgcgcct
     3601 tttccacttt gctcatcagg tcgagaggat aactcggaca ggccgtggcg acatagggta
     3661 cgttgtgggc caccatgatc tccgggacgt tcttcttccc cgtccgctgg cccttgatgg
     3721 ccttgccggc cggagaggtg gtggtggccg ccatgaaggg cgtggcactg gagcgctgga
     3781 tgccggtgtt catgtaggcc tcgttgtcca ggcagaagaa gatcatgtca tggccccgct
     3841 ccatggcccc ggaaagggcc tggaggccga tatccaccgt tccgccgtca ccggcaatgg
     3901 cgacggattt cacatagcgg tcggcgatct tgcccttccg gcgcagggcc ttgagccccg
     3961 cttccacgcc cgacgccacc gcggcggcgt tttcgaaggc cacatggatg tagggcaggt
     4021 tccaggctgt atggggatag gcggaggaga tgatctccat gcaccccgtg gcgttggata
     4081 cgacggtatc ctcgcccagg gccttcagga ggaggcgcag ggccaagacc tcaccgcaac
     4141 cctgacaggc gcgatgcccg gggctgaaaa attccctttt ctcgatcagt ttcggtgcgt
     4201 atatctcgaa gttctctacg gtgttcatca ttccctcacc ccataatatt cataggtttc
     4261 gtctttcttc ttggcggccc gctccaccat ggcgatgaaa tcctccacct tcacgtcccg
     4321 gccacccaga cccatgataa aatcgacgat acgggggcgg tcgtggtcgt ggaacatcag
     4381 tgacttgatc tccgtgccca acggaccgcc ggcgccgccg aaggagatgg cgcgatccat
     4441 gacgatgagg gtctcgcagc ccttgatcgc cgcgcggagt tcgtcgaggg ggaagggtct
     4501 ccaaagtctg atcttcacca atcctatgga ctgccccgcc gcacggagtt catcgacggc
     4561 caccgaggcc gtctcgccca tggagcccat ggtcaggatc agggtttccg cccccttgtt
     4621 cttgtaggtc tccaccggct tgtagctccg gccgaacatc ttgccccatt cgttccagat
     4681 cttgatgatc gtcttcttgg agttgatcag ggcggcgtct ttcgccttca tggtctcggc
     4741 gaatatctcg ggcatattga agcaccccat ggagagggga ttgtcggggt gaagagtggc
     4801 gaagggaacc cgatcgggga ggaaggcgtt tacctcatcc tggcttggaa attccagcgg
     4861 ctcgatcatg tgggtcaact ggaatccgtc catgttgagg accgtgggca gcatgatatc
     4921 gggatgctcg gcgatcttga aggactggat ggtcatgtcg atgacctcct ggccgttttc
     4981 cgcgtgcatg acgatccagc cgctgtcccg ctggggcatg atgtccgtgt ggtcgttcca
     5041 gatgttcagg gggccggaaa cggcgcggtt cgcgttgccc atgaccacgg gcaggcgcat
     5101 ggccgacacg atgggcagga tctcgtgcat gagcatcaag ccctgggagc tggtggccgt
     5161 gtaggcccgg gcgcccgtcg ccgaggcgcc ggcgcaggcg ctgatggagg agtgttccga
     5221 ttccaccgtg atgaactcgg cgtcgatccg cccgtcatgg acgatgtccg aaagatgctc
     5281 ggcgatgtgg gactgggggg tgatgggata gacggcggcg acgtcgatgt tgcagaggcc
     5341 caccgcctcc gccgccgcaa ttccgacttc catgccaaca actttaccca tggtcacacc
     5401 tcctcttcca ccatgacgat ggcctggggc cagcattcat gggcgcagat gccgcagccc
     5461 ttacagtaat ccaggtccgc cgcaaagaat ccctctgccg tttcggaaat gcacgcctca
     5521 gggcagaaca cgtaacagat cccgcattta atacacttct tgttgtccca gatgggtctt
     5581 tgggcgcgcc agcttcccgt cttgtaggta ctggcgttgc ccggttcgaa cacgatgcaa
     5641 ccgggggtga cttcatgcca agcttccggc catttcttct gtttcatgga aacacctcga
     5701 ttatcttttt acttagccag tttaacatca tcataagccc tcttgagggc gttcttgttc
     5761 ttgtcggcga tcttgccgaa ccgctcctgg atgggctcgt caagcgaagc aagcttcaga
     5821 accttggtcg ccttcatcac cgcccccagc atggtggtgt tggcaatagg gacgcccagt
     5881 tcctcccggg cgatatgtgt ggcatccacc gtcgccagct tgcccttata cttgagctgt
     5941 tcccggatgg tcggaaccgg tttcgaggta ttgatgatga gcaatccatc aggcttcaga
     6001 ccgtccgtga cgttcacgag gccgaccagc gagggatcga ggaccacgac cacatcgggg
     6061 tggtaaatgc cggaacggat cttgatgggc ttatcggata cgcggttgta cgccatgacc
     6121 ggcgcccccc gacgctccgg accgaacgcc ggaaatccct gggcgtattt accttcttcg
     6181 atcgccgcca gcgccagcaa ctcgacagac gtgaccgcgc cctggcctcc ccttccatgc
     6241 caccgtattt caatcatgtt catctatctc ccgttttttc ttccttgatt ttattgaatg
     6301 aggcgaaacg aagctttccg tactaaaaat gaaaatttgt gtcaatggta atttgaaaca
     6361 aaccggaaaa aatattgaca agcgccggtg tctcttctat gaacggggca aattcgccgc
     6421 cgaggtcgaa atcaatgaca aaagccgctg aaatgatccg gaaggcactt gccgaaggcc
     6481 gccaatccct tagtgaatac gacgccaaga cgttcctcgg tcttttcggg gtgcccgttt
     6541 ccaaggatat tctggccgca gacgggagag ccgccgcggc cgccgccgag agcctcggct
     6601 atccggtggt actgaaggcc gtcggcaatc gcctcaccca caaaaccgag gtggggggcg
     6661 tggtcctcca cgtgaagacc gcggcggaag tcatcgccga atccgcgcgg ctgatgcgga
     6721 ttcccggctg cgaaaccctc atggtttctc ccctggttgg gggggcgcgg gagcttgtct
     6781 gcggcctcac cagagaccct cacttcgggc cggcggtcat gttcgggctg ggaggcatcc
     6841 tcacggaggt tttgaacgat acggtcttcc gcatcgcccc cctgacggaa gccgatgccg
     6901 aggccatgct ttccggcatc cgggccaaaa agatcctcga tcccttnnnn nnnnnnnnnn
     6961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7021 nnnnnnnnnn nnnnnnnnnn nnnnnnccga tgccgaggcc atgctttccg gcatccgggc
     7081 caaaaagatc ctcgatccct tccgggggga agccgccgcc aaccgtcagg ccttggcaaa
     7141 aatcctcagg gccataggcc ggatcggcgt ggagcatgaa gaggtggcgg aaatcgacgt
     7201 caatcccctg aagatcggcc ccgacggcct cccggtcgcc gtggacgcct tggtcgtttt
     7261 aaaataactc gagaatcgcc tccgccatga gacggtagcc gtcttccgtg gggtggacca
     7321 tatcgtactg gaccagggtc gtgtagggcg tcccggcatc aatgcatctc ttccagtatt
     7381 catggacctt cgccacgggg aggcggaact tctcccccaa ctccgccaac tggtcgtaaa
     7441 actccccgat gatccgggcg tcggcattgt caccgatata actcgacgtc gtcaggatga
     7501 tgtccgcgtc accgtctgcc tggatctcat ctatgatctc cccgatggtg cgccggaact
     7561 gcccggcggt gtagcccatg aaggcgtcgt tgatggcgta ctggacaatg atgcaatcgg
     7621 gattgtattc gaggatgtcg tagcggaggc ggtagaggcc gttgtccgcc gtatcgccgg
     7681 gaatgccctt gcggatcata gtcagaaccg cctggggata gcgggcgggg agcagctccc
     7741 ggaagaaatc gacgtagccc cggcggacca tccacccctg ggtcagggaa tcccccaggg
     7801 cggcgatcat gaccttttca ccccgtttca gtttttctat ggttcgctcc ggcctgatca
     7861 tcgtctccta aattttcagc ggtctttccg ggcccgctcg taaagatccc ctccccaggt
     7921 gtcgttcacc accaccaccg gcatctctgc cagttccagc cggaggatgg cctccggacc
     7981 aagatcctcg tatgcgataa cggcggcgtt gcggacgcac cgggaggtca gggcggccac
     8041 cccgccgatg gcgccgaaat agacggctcg gtggcgcacc atggcggcga tcacctcctg
     8101 agaacgtttc cccttgccga tcataccctt caggccccgc tccatcagga ggggcgcgaa
     8161 ggggtccatc cggtagctcg tcgtcggacc gatggcgccg atgacgcgtc cgggtggcgc
     8221 tggggtcgga ccggcataga agatcaccgc gccgttaaga tcgaaaggca tctcttcccc
     8281 tcggcggagg gcctcccgga ggcggccgtg ggcggcgtcc cgggcggtga agaccacccc
     8341 gctcaggagg accatgtcgc cggctttaag ggcggcgcag tccgcatcgg tgaggggcgt
     8401 cttgagggag tgcgcttccg tcatggctca gatctccgcc tcgcggtgcc gggccgcgtg
     8461 gcactggacg ttcaccgcca cggggaggca ggcgatgtgg caaggcttca tttctacatg
     8521 caccgccagg gccgaggcat tgccgccgaa accctggggg cctccgcccc gccggttgat
     8581 ctccgccagg atctccgcct cgagaagggc caggcgtccg tcgtggcggt ttgtctcccc
     8641 caggggccgg aggagggact ttttggctag gagcaccgcc tcctccatgg cgccgccgat
     8701 cccgaccccg acgatgaccg gcgggcaggg attgggtccc gcccgcagga ccgtctcgac
     8761 cacgtggtcc ctgatcccgt cccgtccggc cgccggcaga agcatggtca cggtgctcat
     8821 attctcgctg ccacccccct tgggcatggc cgtcaggcgg atcgcatcgc cgggaaccac
     8881 atccaggtgg ataaccgccg gggtattgtc accggtattg gccctgctta aggggtcgca
     8941 taccgatttg cggaaataac cctcccggta tgcctgccgc accccctcct caacggcctc
     9001 ccgcagcgcc cccccgacca ctcggacgtc ctgtcccaat tccacgaaaa ccacggccaa
     9061 tccggtatcc tggcaaatcg gcaggcgttc attccgggcg acggcggcgt tttccagtat
     9121 cctctccaga acctggcggc ccagctccgt ttcctcgccg tcccgggccc gacgcagggc
     9181 atcttccaca tcaccgccga ggcaggtgtt ggcacgcaga aacatctccc tgaccttggc
     9241 ggtgatccgg ggggcctcga tattccgaac ggggttcatc gacggcggtc cctcacatcc
     9301 cccggcggaa ttccagggct ttgcccaggg tcatccgatc agtgtacttc agatctcccc
     9361 ccataggaac ccccatggcg atccgggaga tcgccaccgg ccggccggcg aggagtttgt
     9421 tgatgagcag cgccgtggcc tcgccctgga cgtcgggatt cgtggcgatg atcacctccc
     9481 ggacggagtg ggcgtcaacc ctggccagca gttcccggag gtgaaggtcg tcggggccga
     9541 tcccgtccag aggcgccagg accccgtgca ggacgtggta ggtcccgtgg tactccccgg
     9601 tggtctcgat ggcgatcagg gcgtcgggat cctggacgac gcataccgta cccctttccc
     9661 gggccgggtc ggcgcagacg gtgcacagct cttcctccgt gaggttgaag cacaaccggc
     9721 acaggcggat ccgctccttg acctccatga tgctcgcggc caggcgccgg gcatcttcca
     9781 gggattcatg aaggatgaaa ttggcgagtc gggccgcggt cttctcaccg atgccgggga
     9841 agcgggccag ttccgtgatg aggcgctcaa tgggcggggc gtatcgggac atgggacgcc
     9901 gccgctacga caagccgggg atcttcagtc ccccggtgat cttggccatc tccgccgcgt
     9961 tcatctcctg tgccttgcgg accccctcat tgaccgccgc cattacgaga tcctgaagca
    10021 tttcaacatc atcaggatta acaacgtcct tttcgatctt cagggacatc acctcgaact
    10081 tgccgttgac gaccacggag accatgccgc ccccggagga aacctcgacg gtcttcannn
    10141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaaa acgtccatca ccttttgcag
    10261 cagggggttt ctcagggcct cctgcctgat ttcactgttc atgggaaaat tgccgggacc
    10321 attggcaggc ttgccattga cccctgccgc ctccccccgg agggcctcga taaccacctc
    10381 caggtcgggc cgttgaaaga atttccgggc cagctcggcg atggtccgct tctgatgctt
    10441 ctcgatatcc tcaaaaaaga cgttgtggcg gggaaatccg atccgcagga catccccttc
    10501 ccgggccagc agttgccccg gggcgagttt ggagcacaac gacggatggg agcgacggat
    10561 gaaatcctta aagccctccc agccgccccc ttcttccaac gggtggggcg gcgccggcgc
    10621 ggtcttcccg tacggagaca ccgggggttc tttcaccgcc ttcgcctccg gatcttccga
    10681 ccctgcctcc gtttcccccg ccggcgccgg catcccggat cgcaggcggc tctccagggt
    10741 cgtcatgcgg gcgagtatct cgtcgatggg aatcaggggg gtgagacggg ccatgcgcgt
    10801 gagagccatc tccatcacca gacgcggatt ctgggtacgg cgcatcgtct cctcctcggc
    10861 catgaggatc tccaggaggc gctggagggt ctcccgggaa accagcgccg tctgctcctg
    10921 gagcctggac cagtcttccc ggctcatgtc cgtcatcccc tcggcctccg ggacgacctt
    10981 ggccaaaacg agattgcgga agtgttcgag gaggagttgg tagaacaccg gcatgtccat
    11041 accggcgtaa taggcctcgt cgatcaattt caggcattgc gccccgtccc gggccaaaac
    11101 cgccgccgcc acggccacga caaaccgccg gtccgtgagt cccaatagtt cttcgatatg
    11161 ctcgtcggca acggtttccc cagcgtagga gaccacctgg tcgaaaacgc tctgggcatc
    11221 ccgcatgctg ccgtcgccgg cggcggcaat ccacgccacg ccccggtcgc tgatctcgat
    11281 cccctccccg gtggcgatct tccggaggtt gaccgcgatc tcctttcggg agatgcgccg
    11341 gaagtcgaag cactggcagc gggagagaat ggtggcgggg atcttgtggg tctccgttgt
    11401 ggcaaagatg aagacgatgt gggggggcgg ctcctccagg gtcttgagga gggcgttgaa
    11461 ggcctcccgg gtcagcatgt ggacctcatc gatgatatag accttgaaac gggcggaggc
    11521 gggcaaaaac tttatgtttt ccctaagttc tcggacctcg tcgatgccgc ggttggaggc
    11581 gccgtcgatc tcgtggacat ccagggcgat gccatcggtg atctcccggc agtggatgca
    11641 aacctggcag ggcgtcggtg tgggtccccg ctcgcagttc agggccttgg cgagaatccg
    11701 ggcaatggag gtcttgccca ccccccgggg gccgctgaag ataaaggcgt gggcgatgcg
    11761 atcaagtttg acggcgttct ggagggtgcg gaccacatgg tcctggccgg cgacctcctc
    11821 gaatccctgg ggacgccatt tccgggccat gacctgatat tccttagccg ggctgtccat
    11881 atccttgttc gacagttaat aaagatagcc gggtttcccc gcagcacacg tcggttattg
    11941 ctaccgctgc ttccttccgg acctgacggg gtttacaatc cgccgttgca cgggacccgg
    12001 ctattgatcc tttccaaagt tgcggcatta tagaaagggg cttctccatc tgtcaaggtt
    12061 tacttttaaa attgatgccc cctg
//
LOCUS       DAOSMF010000055        12056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_6114_length_12056_cov_26.340138, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000055 DAOSMF010000000
VERSION     DAOSMF010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 12056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12056
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6114_length_12056_cov_26.340138"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /gene="murF"
                     /locus_tag="PLT63_06710"
     CDS             <1..972
                     /gene="murF"
                     /locus_tag="PLT63_06710"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOD98449.1"
                     /translation="LELGTNTPGEIARLARMALPDIGLITNIGLAHLAGFTDLAGVAA
                     EKRDLFRELSAAGTMIVNKDDPFLRDAGRDWQGGVVTFGLHAAANVYADGIVPVLVSG
                     AGVAGQDGTREKYRTETRFTLHLGEERQEITIPVAGEHQIVNALAAAASAWAAGASGE
                     DIALGLGSFTPIPGRMEIIALAGGAFVINDAYNANPSSVREALRTLQQLKGRGRGIAV
                     LGDMLELGPREAAWHEEIGGLLADTGVERVYLRGRLSGATAAGASKHGMPAAAIIVAE
                     DQEEIARDLGAYLQAGDWVLVKGSRRLEMDKVTAALRRAHGEAGGSR"
     gene            969..2048
                     /gene="mraY"
                     /locus_tag="PLT63_06715"
     CDS             969..2048
                     /gene="mraY"
                     /locus_tag="PLT63_06715"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOD98450.1"
                     /translation="MIYHLLYPLHTVFSSFNVFRYITFRTIYAAITALVICFLLGPWL
                     IRKLQEHQIGQSIREDGPNSHLVKQGTPTMGGILIIFAVVTSTLLWANLSVWYIWLVI
                     FVTVSYGLIGFVDDYRKISRKNSRGVPGKVRLAGEVGIALFVGLVLYMKPGFTANVTI
                     PFFKTALPNLGWGYIFLAAFIIVGAANAVNLTDGLDGLAIGPITICFMTYLFFAYFAG
                     NIKIASYLQIPYVAGTGELSIFCGAMVGAGLGFLWYNTYPAQVFMGDVGSLSLGGALG
                     TLAVLTKQEILLAIVGGIFVLETFSVIFQVGWFKMSGGKRIFRMAPIHHHFELKGWAE
                     PKVIVRFWIISILLALVALSTLKLR"
     gene            2057..3391
                     /gene="murD"
                     /locus_tag="PLT63_06720"
     CDS             2057..3391
                     /gene="murD"
                     /locus_tag="PLT63_06720"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOD98451.1"
                     /translation="MELQGKNIAVIGLAKTGLATAEFLLGRGAKVTATDMNPQSLAAA
                     ALPGMKTAPHDPEILTGVDLVVPSPGVPPANPILAAAAGAGIPILSELELAGRFLRTP
                     LIAITGTNGKTTTTALIGHILGQSGRRVFVGGNIGTPLIGYAGGPQEDHWAVIEVSSF
                     QLQWSETFHPAVAVLLNVTADHLDYHGSFTAYRAAKERLFANQTAADTAILNGDEPGT
                     AALAPRLAARTLRFHSGGTAATGMYPEGEQLVYVSPTGEKEIYPRSMVKLPGTHNLEN
                     VMAAVMAARSCGCTPAEIAAAVASFAGFPHRIEYAGQWRGVSFYDDSKGTNVDAVRRA
                     LETFAAPVILLMGGRDKAGDFRPLIPLVRQKAKRLVLFGEAREAIGVVLGGLVATDSR
                     PTLREGVAAAAAGATPGDVVLLSPGCASFDEFRDYKARGKFFQDQVKALAHD"
     gene            3384..4490
                     /gene="ftsW"
                     /locus_tag="PLT63_06725"
     CDS             3384..4490
                     /gene="ftsW"
                     /locus_tag="PLT63_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HOD98452.1"
                     /translation="MIKFDSTDRSPDIILLIVTLILVTVGTVMIYSSSSIMAMERFKD
                     GQFFLKKQMIFVVIGLGAMIFMTRIPYEELKKFAYPGVIISLVLLTLVLIPKIGVRVG
                     GASRWLKVGFFAFQVTELVKIFVVLFLAHFLTRKAMYAKELMRGVVIPLVVTMLLVGL
                     IVIEPDFGTAVLITMILMLMLYLAGSRVRYLAGLVGMLIPVGIGLLFYKSYRVERLMT
                     FLDPWRDPQNSGFQIIQSLISFGSGGTFGVGVGDGRQKLFYLPEPHTDFILSIIAEES
                     GFIGVAIIICLFAVFVIRGFLISFKAPDKFSTLLAAGLTMIIALEAFVNIAGVMGLIP
                     LKGLALPFLSYGGTSLIMSLTIVGILLNISAHEQ"
     gene            4509..5609
                     /gene="murG"
                     /locus_tag="PLT63_06730"
     CDS             4509..5609
                     /gene="murG"
                     /locus_tag="PLT63_06730"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HOD98453.1"
                     /translation="MIIAGGGTGGHLFPGVAVAEAFMNLDRLHEVLFVGTERGLEKRV
                     LPELGYPLATLDVEGIKGRSWGNSLQAALKIPKSMMQAFKIMKKFSPHVVLGVGGYAS
                     GPAVLTAHFLGIRTAIAEQNAIPGVTNRILANFVDRIFLSFPDPDGEFPARKSVVAGN
                     PIRASFVAAAADHQQQQREREDIIFDILVFGGSQGAHAVNMAVLDAAALLGELREKIR
                     IVHQTGAKDVPEVRAGYEKLEITAAVHPFIMDMAAAYREADLLICRAGATSVAEITAM
                     GKAAILIPFPFAIHDHQTKNAEVLVRAEAALMIPERELTGAKLAAAIRELAADPERIA
                     RMSARSRSLGRPEAAAEIAAACVAMAERRGLH"
     gene            5657..7060
                     /gene="murC"
                     /locus_tag="PLT63_06735"
     CDS             5657..7060
                     /gene="murC"
                     /locus_tag="PLT63_06735"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOD98454.1"
                     /translation="MTDTRRMDGMRRKVKTVHFVGIGGIGMSGIAEVLLNLGYGITGS
                     DLGQTDITQRLATLGAVIFKGHDEIHLGDADVVVTSTAVKSDNPEVLAAHRRNIPVIP
                     RAEMLAELLKMKFSIAVSGSHGKTTTTSMISTVLGQGGLDPTMVIGGKLASIGSNAKM
                     GGGEIIVAEADESDGSFLKLGPCIAVITNIDREHLDHYRDIDEIKAAFLQFANIVPFY
                     GSTVLCLDNLHIREILPQIKRKTITYGLEPPADYRAADINIRGTWTSFNVLYREELLG
                     AAVLNVPGLFNVYNALATIAVAREIDMDFPAITAGLKTFTGVQRRLEVKGIGGGVTVV
                     DDYGHHPTEIRETLAAARTVWPGRLLVVFQPHRYTRTRALFEEFTGAFSDADLLVITD
                     IYAASEAPIPGVNSENLAAAIRKQEQQEVMYIADFDAISDRLLALARPGDVIITQGAG
                     SVWKIGEEYLQKAAAKR"
     assembly_gap    7179..7278
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7279..7830
                     /gene="murB"
                     /locus_tag="PLT63_06740"
     CDS             <7279..7830
                     /gene="murB"
                     /locus_tag="PLT63_06740"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:TIGR00179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOD98455.1"
                     /translation="LEFCAGIPGTVGGAVTMNAGAYGHEIKDVLSELIMMDRMGKTSD
                     WRRDRLQFFYRRFVPPIEDGLIMAATFRLAPGNPEEISNKIMEIKDLRRRKHPLEYRS
                     AGSVFKNPPGQPAGRLIEETGLKGMRCGGAQISEKHGNFIVNRGGATAEDILALIGII
                     QERVGREKNVRLETEVVVVGEGP"
     gene            7827..8693
                     /locus_tag="PLT63_06745"
     CDS             7827..8693
                     /locus_tag="PLT63_06745"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HOD98456.1"
                     /translation="MKIWRKEKVKYRNYREEVKKYRRRRQTGTVLMEAAKASLILTAI
                     GVTAAALVFGYLYLISHPYFAIREINVRGCKELTEKEILTAAAVRTRQNIFAVSTKEV
                     QEKIRANPWIKEVSVGRELPDRLIIDLKERNAVSLVKKDDGFYLMDFDGITFKKLELT
                     DDMDIPILTGYQGKGEESRLLLMRTMELLRHLATKNDYPNIKNVSEINGNERYGLSVF
                     TNSGLCLFLGFDNYESKFKRLFPVMVDLERRNLKTGHLYIDLSDPVKITVQRREVLAP
                     ALEATGKKGFKT"
     gene            8713..9933
                     /gene="ftsA"
                     /locus_tag="PLT63_06750"
     CDS             8713..9933
                     /gene="ftsA"
                     /locus_tag="PLT63_06750"
                     /inference="COORDINATES: protein motif:HMM:NF007009.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOD98457.1"
                     /translation="MAKKANIIVGLDIGTTKTCAVVGELTDQGIDIIGIGSSPSEGLR
                     RGVVVNIDSTVEAIKKAVEEAEKISGNQIRSVYVGIAGGHIKGQNSLGIIAVKEREVD
                     EEDVQRAVEASKAIAIPLDREILHTLPQSYIVDDQDGIRDPIGMSGVRLEAKVHIVTG
                     ASASIQNIAKSVSRVGLDIEEIVLEQLASSEAVLSSDEKDLGVAMLDIGGGTTDIAVF
                     SEGSIKHTAVLPVGGNYVTSDIATGLRTPAGEAEKLKIKYGCAYSPLIPKDEAIEVPS
                     VGGREPREVSRQILGRIIEPRMEEILSLAQKEIVRSGYEELMAAGVVLTGGASIMEGA
                     PELAEHVFNLPVRRGQPTGIGGLTDIVNSPMYATGVGLVLYGIKHVSRDYKRDGGSLG
                     KWFQGIKKWFIEFF"
     gene            9949..11100
                     /gene="ftsZ"
                     /locus_tag="PLT63_06755"
     CDS             9949..11100
                     /gene="ftsZ"
                     /locus_tag="PLT63_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOD98458.1"
                     /translation="MFELTDLSTTNTARIKVIGIGGGGSNAVNTMISYNLQGVDFIVA
                     NTDTQALGASTSPIKIQLGSEVTKGLGAGANPEVGRQAATETKDRLREHLEGADMVFI
                     TAGLGGGTGTGGAPVIAEICKDLGVLTVAVVTKPFQFEGKRRNYQAEEGVDELRRIVD
                     TLIVVPNQRLLSLGGRNLSLLEAFKKADDILYHAVKGISDLIIIPGLINLDFADVKNT
                     MSNMGLALMGTGTANGENRAVEAAQRAISSPLLEDNSIHGAHGVLLNITGGPDMSLFE
                     VNEASSLIQAEAHEDANIIFGTVIDENMGDEIRITVIATGFEDISGRKNTVVKMSSMG
                     GMKRDDLSKPAYLRKDKVIDSAMTVKMGLIEDNEEPDLEIPTFLRRQAD"
     gene            11110..>12056
                     /locus_tag="PLT63_06760"
     CDS             11110..>12056
                     /locus_tag="PLT63_06760"
                     /inference="COORDINATES: protein motif:HMM:NF040068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98459.1"
                     /translation="MSWAIRKKFRELLNREDGYEKKSWGSALTICLAYPNRYRTGMGN
                     LGFQTVYKLLNRQSAVICERVFLPDPGDEASFSPGPLPLFSLESQRPLRDFDIVAFSL
                     SFENDYPHILKILSLGGIPLLAGERTAGDALIIAGGICVTMNPEPLADFFDLFLIGEA
                     EALLPDFLAALRHHREKGDKKDELLLGLQRDVPGAYIPRLYEVTYHNDGLIAAMTPVV
                     PFLPRKIGKKWAADIQDLATESVIMAPDAEFGAMYLMEISRGCSRGCRFCAAGYLCRP
                     VRFRRREYLQGAIARGMAKGKKIGLIGTAVSDHPQLIEI"
BASE COUNT         2423 a         3647 c         3681 g         2205 t
ORIGIN      
        1 ctggagctgg ggacgaacac cccgggggag atcgcccgac tggcccggat ggccctgccg
       61 gacatcggcc tgatcaccaa tatcggcttg gcccatctgg cgggattcac ggacctggcc
      121 ggggtggcgg cggagaaaag agacctgttc cgggagctgt cggcggcggg gacgatgatc
      181 gtaaacaagg atgatccctt cctccgggat gccggacggg attggcaagg cggggtcgtc
      241 accttcggat tgcatgccgc ggcgaacgtt tacgccgacg ggatcgtccc ggtgctggtc
      301 tccggggctg gcgtcgccgg gcaggacggg accagggaaa agtaccggac ggaaacccgt
      361 ttcactctcc atctggggga ggaacggcag gaaataacca tacccgtcgc gggcgaacat
      421 cagatcgtaa atgcccttgc tgctgccgca tccgcctggg cggccggcgc ctccggagag
      481 gatatcgccc ttggcctggg cagtttcacc ccgattccgg ggcggatgga aatcattgcc
      541 ctggccggcg gcgccttcgt catcaacgat gcctacaatg ccaatccgtc ctcggtccgg
      601 gaggccctcc ggaccctgca acagctcaag ggacggggac gcgggatcgc ggtcctgggg
      661 gacatgcttg aactgggacc ccgggaagcc gcctggcatg aggaaatcgg gggccttctg
      721 gcagacaccg gagtggaacg ggtttatctc cggggaaggc tctcaggagc gacagcagca
      781 ggggcctcga aacacggcat gccggcggcg gctatcattg tggcggagga tcaggaagaa
      841 atcgcccggg acctcggggc atatctccag gcgggggact gggtcctcgt caagggctcc
      901 cggcggctgg agatggacaa ggtgaccgcg gcgctccgcc gggctcatgg ggaagcggga
      961 ggcagccgat gatctatcat ctcctctatc cactccatac ggtcttctcc tctttcaacg
     1021 tcttcaggta catcaccttc cggacgatct atgcggccat caccgccctg gtgatctgtt
     1081 tcctcctggg accctggctg atccgcaagc tccaggagca ccagatcggc cagtcgatcc
     1141 gggaggacgg cccgaacagc cacctggtga agcaggggac cccaaccatg gggggaatcc
     1201 tgatcatctt tgccgtggtg acctccaccc tcctgtgggc caacctctcc gtctggtaca
     1261 tctggctggt catcttcgtc acggtgagct acgggctgat cggcttcgtg gacgactacc
     1321 ggaagatctc ccggaaaaac agccgggggg tgccggggaa ggtccggctc gccggcgagg
     1381 tcggcatcgc cctcttcgtg ggtctggtgc tgtacatgaa gccgggattc accgccaacg
     1441 tcaccatccc cttcttcaag accgccctgc ccaacctggg ctgggggtac atatttctgg
     1501 cggccttcat catcgtcggc gccgccaacg ccgtcaacct caccgacggt ctggacggcc
     1561 tggccatcgg ccccatcacg atctgcttca tgacctatct gttcttcgcc tacttcgcgg
     1621 ggaacatcaa gatcgcctct tatctccaga tcccctacgt ggccggcacg ggggaactgt
     1681 cgatcttctg cggcgccatg gtgggggccg gactgggctt tctgtggtac aacacctatc
     1741 ccgcccaggt attcatgggc gatgtggggt ccctgtccct ggggggcgcc ctggggaccc
     1801 tggccgtcct cacgaaacag gagatcctcc tggccatcgt gggcggcatc ttcgttctgg
     1861 agaccttttc cgtgatcttc caggtgggct ggttcaagat gtccgggggg aagcggatct
     1921 tccggatggc ccctatccat catcatttcg agctcaaggg ctgggcggag ccgaaggtca
     1981 tcgtccgttt ctggatcatc tccatcctcc tggccctggt ggccctgagc accctgaagt
     2041 tgaggtaggc gcgattatgg agctgcaagg aaagaacatc gccgtcatcg ggctggccaa
     2101 aacggggctg gccacggcgg agtttctcct cggccggggg gcgaaggtga cggcgacgga
     2161 catgaatccc cagtctctgg ccgctgcggc gctgccgggg atgaagaccg caccgcatga
     2221 tccggagatt cttacggggg tggacctcgt cgtcccctcc cccggcgtgc caccggcgaa
     2281 tcccatcctt gccgcggcgg cgggggcggg catcccgatc ctcagcgagc tggaactcgc
     2341 gggacgcttt ctccgcacgc ccctcatcgc catcaccggc accaacggga agaccacgac
     2401 cacggcgctc atcggccaca ttctaggaca aagcggccgg agagtctttg tgggcggaaa
     2461 catcggcacc cccctcatcg gctacgccgg cggcccccag gaggaccact gggcggtgat
     2521 cgaggtgagc agcttccagc tccagtggag cgaaacgttc catcccgccg tggcggtcct
     2581 cctcaatgta accgcggatc atctggacta ccatgggtca ttcaccgcct accgcgcggc
     2641 caaggagcgc ctcttcgcca accagacggc agcggacacg gcgatcctca acggcgacga
     2701 accggggacg gcggcgctgg ccccgcgcct ggccgcccgg acgctccgtt tccacagcgg
     2761 cggcaccgcg gcaaccggga tgtacccgga gggagagcaa ctggtctatg tctccccgac
     2821 gggggaaaag gaaatctatc cccggtccat ggtgaagctg ccggggaccc acaacctgga
     2881 gaacgtcatg gcggcggtta tggcggctcg gagctgcggc tgcaccccgg cggagatcgc
     2941 tgcggcggtc gcgtccttcg ctggcttccc ccatcgtatc gaatacgcgg ggcagtggcg
     3001 gggtgtctcc ttctacgacg actccaaggg gaccaacgtg gacgccgtcc ggcgggcgct
     3061 ggaaacattt gcggcgccgg tgatcctcct catgggggga cgggacaagg cgggagactt
     3121 ccgacccctg atccccctgg tgcggcagaa ggccaaacgg ctcgtcctct tcggggaggc
     3181 ccgggaggcc attggcgtgg tcctcggcgg cctcgtggcg acggattccc gcccgaccct
     3241 ccgggagggc gttgccgcgg cggcggcggg ggcgacgcct ggggacgtgg tcctcctgtc
     3301 gccggggtgc gccagcttcg acgagtttcg ggattacaag gcccggggga aattctttca
     3361 ggatcaggta aaggcccttg cccatgatta aattcgactc caccgacaga agtccggaca
     3421 tcatcctgct catcgtcacc ctcatcctcg tcaccgtggg gacggtgatg atctacagct
     3481 ccagctccat catggccatg gagcgcttca aggacgggca gttcttcctc aagaaacaga
     3541 tgatcttcgt cgtcatcggc ctgggggcta tgatcttcat gaccaggatc ccctatgaag
     3601 agctcaagaa gttcgcttat cccggggtga tcatctccct ggtcctcctc accctggtcc
     3661 tcatccccaa gatcggcgtc cgggtcggcg gcgcctcacg atggctgaag gtgggttttt
     3721 tcgccttcca ggtcacggag ctggtgaaga tcttcgtcgt actgtttctc gcccatttcc
     3781 tcacccggaa ggcgatgtat gccaaggaac tgatgcgggg ggtcgtcatc cctctggtgg
     3841 tgaccatgct cctggtgggg cttatcgtca tcgagccgga tttcggcacc gccgtcctga
     3901 tcaccatgat cctcatgtta atgctctacc tggcgggaag ccgggtccgc tatctcgcgg
     3961 gactcgtagg tatgctcatc cccgtgggga taggcctgtt gttctacaag agttaccggg
     4021 tggagcgact gatgaccttt ctcgacccct ggcgggaccc ccagaactcg ggatttcaga
     4081 tcattcaatc cctcatctcc ttcggatccg gggggacctt cggggtgggc gtgggcgacg
     4141 gccggcagaa gctcttctac ctgcccgaac ctcatacgga tttcattctc tccatcatcg
     4201 ccgaggagag cggtttcatc ggcgtggcca tcatcatctg cctgttcgcc gttttcgtga
     4261 tccggggatt cctgatctcc ttcaaggccc cggacaagtt cagtacgctc ctggcggcgg
     4321 ggttgaccat gatcatcgcc ctggaggcct ttgtcaacat cgccggcgtc atggggctca
     4381 tccccctgaa gggactcgcc cttccgtttc tgagttacgg tgggacatcc ctcatcatga
     4441 gcctcaccat cgtgggcatc ctgctcaaca tctcggccca tgaacaatga ggggagaaaa
     4501 cagtgaagat gatcatagcc ggaggcggca cgggaggaca tctctttccg ggcgtggccg
     4561 tcgccgaggc ctttatgaat ctggatcgcc tccacgaggt gctcttcgtg ggtacggagc
     4621 gggggctgga aaagcgggtc ctcccggagc tggggtatcc cctggccacg ctggacgtgg
     4681 agggcatcaa ggggcgttcc tgggggaatt ccctccaggc cgcactgaag ataccgaaaa
     4741 gtatgatgca ggcatttaaa ataatgaaga aattttctcc ccatgtcgtc ctgggcgtag
     4801 gagggtacgc ctcggggccg gcggttctca ccgcccattt cctcgggatc aggacggcca
     4861 tagccgagca gaacgccatc cccggggtga cgaaccgtat cctggcaaac tttgttgatc
     4921 ggatctttct gtccttcccg gatccggacg gggagtttcc cgcccgcaaa tccgtggttg
     4981 ccggcaatcc gatccgagcc tccttcgtgg cggcggccgc agaccaccaa caacaacaac
     5041 gggagcggga ggatattatt ttcgacatcc tcgtattcgg gggcagccag ggggcccatg
     5101 ccgtcaatat ggcggttctg gacgccgccg ctcttttggg ggagcttcgg gaaaagatcc
     5161 ggatcgttca ccagaccggg gccaaagacg tcccggaggt gcgggcggga tacgaaaagc
     5221 tggaaataac ggcggctgtc catcccttta tcatggacat ggccgccgcc taccgggagg
     5281 cggacctgct gatctgccgg gccggggcga cttcggtggc ggaaattacc gccatgggca
     5341 aggcggcgat cctgatcccc ttccccttcg ccatccacga ccatcagacg aagaacgccg
     5401 aggtcctcgt ccgagccgaa gcggcgctga tgatcccgga acgggagctg accggcgcga
     5461 aacttgccgc cgccatcaga gaactggccg ccgatccgga gcgaatcgca cgcatgagcg
     5521 cccggtcccg gtccctgggc cgcccggagg ccgctgcgga gatcgccgcc gcctgcgtcg
     5581 ccatggcgga acggcggggc ttgcattaac attgccgggg gagtataagc ggcgaatttt
     5641 ttaaaaggtg aagaccatga cagacaccag aagaatggac ggcatgcggc ggaaggtaaa
     5701 gacggttcat ttcgtcggca tcggcggcat cggcatgagc ggcatcgccg aagtgctcct
     5761 gaacctcggt tacggcatta cgggctcgga tctgggacag acggacatca cccagcgcct
     5821 cgccaccctg ggcgccgtga tcttcaaagg gcatgacgag attcatctgg gcgacgccga
     5881 cgtcgtggtg acctccacgg ccgtaaagag cgacaacccc gaggtcctgg cggcccaccg
     5941 gcgcaacatc cccgtcatcc cccgggcgga gatgctggcc gaactcctga agatgaaatt
     6001 ttccattgcc gtctcgggca gtcacggcaa gactaccacc acctccatga tctccacggt
     6061 cctcggccag ggcggcctgg acccgaccat ggtcatcggc ggcaagctgg ccagcatcgg
     6121 cagcaacgcc aagatgggcg gcggggagat catcgtcgcc gaggccgatg aaagcgacgg
     6181 ctcctttctc aagctcggcc cctgcatcgc cgtcatcacc aatatcgacc gggagcatct
     6241 ggaccactat cgggacatcg acgagatcaa ggcggccttc cttcaatttg ccaatatcgt
     6301 ccccttttac ggttccaccg tcctttgtct cgacaatctc cacatccggg agatcctgcc
     6361 ccaaataaaa aggaagacga tcacttacgg tctggaacca ccggcggact accgggccgc
     6421 cgacatcaac atccggggta cctggacctc cttcaacgtc ctgtaccggg aggagctcct
     6481 gggagcggcc gttctcaacg tccccggatt atttaatgtt tacaatgccc tggccaccat
     6541 cgccgtggcc agggaaatcg acatggattt tccggccatc accgccggtt tgaagacctt
     6601 caccggggtc cagcggcgtc tggaggttaa gggaatcggc ggcggggtta cggtggtgga
     6661 cgactacggc caccatccga cggagatccg ggaaaccctg gcggcagccc gcaccgtctg
     6721 gccggggcgg ctcctcgtgg tcttccagcc ccatcggtac acccggaccc gggccctctt
     6781 cgaggaattt accggcgcct tcagcgacgc cgacctcctg gtgatcaccg acatctacgc
     6841 cgccagcgaa gccccgatcc cgggggtcaa ttcggaaaat ctggcggcgg ccatccggaa
     6901 acaggagcag caggaagtga tgtacatcgc cgatttcgat gccatttcgg accggctgct
     6961 tgccctcgcc cggcctggcg acgtaatcat aacccagggt gcgggatcag tgtggaaaat
     7021 cggtgaagaa tatctccaga aggcagcggc aaagagatga agaaaggcgt tcgtgaggaa
     7081 ttgaagcgca tcgccgggga tggcgtccgg ttcgatgagc ctatggccaa ccacacctcc
     7141 ctgggggtgg ggggacccgc cctggcggtc gtttatccnn nnnnnnnnnn nnnnnnnnnn
     7201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7261 nnnnnnnnnn nnnnnnnnct ggaattctgc gccggcatcc caggcaccgt gggcggcgcc
     7321 gtgactatga atgccggggc ttacggccat gagatcaagg acgtgctgag tgaactgata
     7381 atgatggacc ggatgggaaa gaccagcgac tggcggcgag accggctcca gtttttttac
     7441 cgccgctttg tgccgcccat cgaagacgga ttgatcatgg cggcaacctt ccgcctcgcc
     7501 ccgggcaacc cggaggaaat aagcaataag attatggaaa taaaggactt gcgaaggcgg
     7561 aagcaccctc tggaataccg gagtgccgga tcggtcttca agaatccccc cgggcagccg
     7621 gcaggccggc tcattgagga aacgggtctc aaaggcatgc ggtgcggtgg cgcccaaata
     7681 tctgaaaaac atggaaattt catcgtcaac cggggcgggg ccacggctga ggacatcctc
     7741 gcgctgatcg gaataatcca ggagcgggta ggccgggaaa agaatgtccg cctggagacg
     7801 gaagtcgtcg tggtgggaga aggtccgtga agatctggcg taaggaaaaa gtaaagtatc
     7861 gtaattaccg ggaggaggta aaaaagtacc ggcggcggcg ccagacggga accgtcctga
     7921 tggaggcggc caaggcctcc ctgatcctga cggccatcgg cgtcaccgcg gcggcgctgg
     7981 tcttcggata cctctatctc atcagccatc cctacttcgc catccgggag atcaacgtgc
     8041 gcggctgcaa ggaattgacg gaaaaagaga tcctcaccgc cgccgccgtc cggacccgtc
     8101 agaacatctt cgcggtgagc acgaaagagg tgcaggagaa aatccgcgcc aacccctgga
     8161 tcaaagaagt gtcggtagga cgggagcttc cggatcgtct gatcatcgac ttgaaggagc
     8221 gcaacgccgt gtcgctggtg aagaaggacg acggcttcta tctcatggat ttcgacggga
     8281 tcaccttcaa aaagctggaa ttgaccgacg acatggatat acccatcctc acgggttacc
     8341 agggtaaggg cgaagaaagc cggctcctgc ttatgcggac catggaactg ctccgccatc
     8401 tggcgacaaa aaacgattat ccgaacataa aaaacgtctc cgaaataaac ggcaacgagc
     8461 gttacggact gtcggtcttc accaacagcg ggttgtgcct ctttctgggt ttcgataatt
     8521 atgaaagtaa attcaaacgg ctgtttccgg tgatggtcga tctggaacgc cgcaatctta
     8581 agaccggtca tctctatatc gatctgagcg acccggtgaa gatcaccgtc cagcgtcggg
     8641 aggtcctggc gccggcgctg gaggccacgg gcaaaaaagg attcaagaca taaaggaaag
     8701 aggaagacag gcatggctaa aaaagcgaac atcatcgtcg gtctggacat cggcaccacc
     8761 aagacctgtg ccgtcgtcgg cgaactcacc gatcagggaa tcgatatcat cggtatcggt
     8821 tccagcccct ccgagggcct gcgcaggggg gtcgtggtaa acatcgacag caccgtggag
     8881 gccataaaaa aggcggtgga ggaagccgaa aaaatctccg gcaatcagat ccgctccgtc
     8941 tatgtcggca ttgccggcgg gcacatcaag gggcaaaaca gcctggggat cattgccgtt
     9001 aaagaacggg aagtggatga ggaagatgtc cagcgggccg tggaggcctc caaggccatt
     9061 gccattcccc tggatcggga gattctccat accctgcccc aatcctacat cgtcgatgat
     9121 caggacggca tccgggatcc catcgggatg tccggggtcc gtctggaagc caaggtgcac
     9181 attgtcaccg gcgcctccgc ctccatccag aacattgcca aatcggtaag ccgggtcggc
     9241 ctggacatcg aagagatcgt cttggaacaa ctggcctcca gcgaagcggt gttgagcagc
     9301 gacgagaaag acctcggggt ggccatgctg gacatcggcg gcggcaccac ggacatcgcc
     9361 gtcttctcag agggcagcat caagcatacc gcggtgctcc ctgtgggcgg caactatgtg
     9421 accagtgaca tcgccaccgg cctcagaacc cccgccgggg aagcggaaaa gctgaaaata
     9481 aaatacggct gcgcctattc ccccctgatt cccaaggacg aagccatcga ggtccccagc
     9541 gtgggtgggc gggaaccccg ggaggtatcg cgccagatcc tgggccgcat catcgagccc
     9601 cggatggagg agatcctctc tttggcccag aaggagatcg tccggtccgg ttatgaggaa
     9661 ctcatggccg ccggggttgt cctcaccggc ggcgcctcga tcatggaggg ggcgcccgag
     9721 ctggctgaac atgtcttcaa tctgccggtc cgcaggggac aacccacggg gatcggcggg
     9781 ctcaccgata ttgtcaacag tcccatgtat gccaccggcg tgggactcgt tctatacggc
     9841 atcaagcatg tatccagaga ttataagcgg gacggcggat cattaggcaa atggttccag
     9901 ggcataaaga aatggtttat cgaattcttt taatggggag gaaagaaaat gtttgaactt
     9961 acggacttat caactaccaa cacggccagg atcaaggtta tcggcatcgg tggaggtggc
    10021 agcaatgcgg tgaacacgat gatctcctac aatctccagg gggtcgattt catcgttgcc
    10081 aacaccgaca cgcaggccct gggcgcatcc acgtcgccga tcaagatcca gttaggatcg
    10141 gaagtcacca agggactcgg ggcgggagcc aatccggagg tgggacggca ggccgccacg
    10201 gaaaccaaag accgcctccg agaacatctt gaaggcgccg acatggtgtt catcaccgct
    10261 ggactcggcg ggggcaccgg aacgggtggg gcgccggtaa tcgctgagat ctgcaaggat
    10321 ctcggcgtcc tcaccgtggc ggtggtcacc aagcccttcc agttcgaagg caagcgccgg
    10381 aactatcagg ccgaggaagg ggtggacgag ctgcggcgga tcgtcgacac cctcatcgtc
    10441 gtccccaacc agcggctcct gagcttggga gggcgtaatc tctccctcct ggaggccttc
    10501 aagaaggccg acgacatcct ctatcatgcc gtcaagggga tctccgacct cattatcatt
    10561 ccgggactca tcaacctgga tttcgccgac gtgaagaaca ccatgtccaa catggggctg
    10621 gccctcatgg ggaccggcac ggccaacggt gaaaaccgtg ccgtggaggc ggcccagcgg
    10681 gcgatctcct cgcccctcct ggaggacaac agtatccatg gggcccacgg agtgctcctc
    10741 aatatcaccg gcggtcccga catgagcctc ttcgaagtca acgaggcctc ttccctcatc
    10801 caggccgagg cccacgaaga cgccaacatc atcttcggaa cggtgatcga cgagaacatg
    10861 ggcgacgaga tccgcatcac cgtcatcgcc actggtttcg aagacataag cggccgtaag
    10921 aacactgtgg tcaaaatgtc ttccatgggc ggcatgaagc gggacgacct ttccaaaccc
    10981 gcctacctcc ggaaagacaa ggtaatcgac agcgcgatga cggtaaagat gggactcatc
    11041 gaagacaacg aggagcccga tttggagatc cccactttcc tgagaaggca ggcggactga
    11101 cggagttcca tgtcctgggc gatccgcaaa aagttccggg aattgttaaa ccgtgaagac
    11161 ggttatgaaa aaaagtcctg gggaagcgcc ctcaccatct gcctggccta tccgaaccgc
    11221 taccggacgg gcatgggcaa cctgggcttc cagacggtat ataagctgtt gaaccggcaa
    11281 tccgctgtta tctgcgaacg ggtttttctt cccgaccccg gggacgaagc atctttctcc
    11341 ccggggcccc tccccctttt cagtctggaa tcccagcgcc ccctccggga tttcgacatc
    11401 gtcgcctttt ccctatcctt tgaaaacgac tatccccaca tcctcaagat cctttcactg
    11461 ggcggcattc ccctccttgc cggggaacgt accgccgggg acgccctcat aatcgccggc
    11521 ggcatctgcg tcaccatgaa tcccgaaccc ctggcggact ttttcgacct cttccttatc
    11581 ggcgaggcgg aagcgctcct ccccgacttt ctcgccgcgc tacgccacca ccgggaaaag
    11641 ggtgataaaa aagatgagct gctcctcggt ttgcagcggg acgtccccgg ggcatacata
    11701 ccgaggctgt atgaagtcac ctatcacaac gatggcctca tcgccgccat gacccccgtc
    11761 gttcctttcc tcccccggaa gatcggaaaa aaatgggcgg cggatatcca ggatctggcc
    11821 acggaatcgg taatcatggc gccggatgcg gaattcgggg ccatgtacct catggagatc
    11881 agccgcggct gcagccgcgg ctgccgcttc tgcgccgccg gttatctctg ccggccagtg
    11941 cggttccgga ggcgggaata cctccagggc gccatcgccc gggggatggc caagggaaaa
    12001 aagatcggtc tcatcggcac ggccgtctcc gatcatccgc aactgattga aatctg
//
LOCUS       DAOSMF010000056        11983 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_6158_length_11983_cov_23.092807, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000056 DAOSMF010000000
VERSION     DAOSMF010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 11983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11983
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6158_length_11983_cov_23.092807"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(377..1681)
                     /gene="tyrS"
                     /locus_tag="PLT63_06765"
     CDS             complement(377..1681)
                     /gene="tyrS"
                     /locus_tag="PLT63_06765"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOD98460.1"
                     /translation="MKNVYDVFLERGFVEQVTDDEAIGRLLDEPGVTCYIGFDPTATS
                     LHIGSLVPIMALAHMQRHGHRPIALVGGGTGLIGDPSGKTEMRQILTRDKIDYNARCL
                     QQQLARYLDFGAGRALLLNNADWLTKLNYIEFLRDIGRHFSVNKMLAAESYKMRLEKG
                     LNFIEFNYMLLQAYDFLYLFKNYDCIVQMGGNDQWGNMLAGSDLIRRMEGKAAHSLTF
                     PLITTSAGHKMGKTERGTVWLDSALTFPYEYYQYWVNTEDEDVERFLCLFTFLPVEEI
                     REVRNLADARLNMAKSVLAFEATKITHGETAALEAWRSSVRAFQARPVDAELFPSSSI
                     PRKRSITLINGGTDDLSAIPSLKKSHADMEKGIPAYELFHETGLCASRGEARRLIEQG
                     GAYINENPVVSFDEKITESHINDQGLVLLRKGKKKHFLIVVR"
     gene            complement(1740..2528)
                     /locus_tag="PLT63_06770"
     CDS             complement(1740..2528)
                     /locus_tag="PLT63_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HOD98461.1"
                     /translation="MRLLFVGDIVGKPGRRAVHDLLPELVRNRQIDFVIANGENAAAG
                     FGLTREIIEELFGNQIDVLTTGNHVWDKKEILDCINDYESLLRPANYPAPAPGRGSVV
                     MPAVNGRHVGVVNLMGRIFMHPLECPFRTAEREIERLRMRTQVIIVDIHAEATSEKIA
                     LGWFLDGKVTAVLGTHTHVQTADERILPSGTAYITDVGMTGPFDSVIGTRKEIILERF
                     LSQLPNKFEVAKGDVRLQAVLMEIDVKSGTALQIERLSLPLHQE"
     gene            complement(2566..4119)
                     /gene="rny"
                     /locus_tag="PLT63_06775"
     CDS             complement(2566..4119)
                     /gene="rny"
                     /locus_tag="PLT63_06775"
                     /inference="COORDINATES: protein motif:HMM:TIGR03319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOD98462.1"
                     /translation="MIMILIIVAAVAFGLIAGLGVNHFLLKKKRAASESLAGRIVEEA
                     KKEAENIKKESLLQAKENLLKVKTEFEKDTRDRKMELDAVERRIRAKEENLEKRMDAL
                     TQKENAIEIRERGLQQKEQTLHDKHEHLNRSIEEQKEKLQKIACISSEDAKNMLIQSM
                     ESEARIEAATTVRKIEEEAKRTADKKASEIIAYAIQRYSGEFVTENTVSVVNLPSDEM
                     KGRIIGREGRNIRAIEAATGIDLIVDDTPEAVVLSSFDPVRREVARISLERLISDGRI
                     HPGRIEDIVKKVRTEVDTIIRETGERTCFDVGVHDVNPEIVTLLGSLKFRKSFSQNVL
                     QHSIEVAHLTGMMAAELKLNVKEAKRAGLLHDIGKAIDHEIEGPHATIGADFARRFGE
                     SPRIVQAIATHHDDGRTNTLLGVLVQAADTLSAARPGARREMLESYVKRLEELEAIAN
                     SFNGVDRCFAIQAGREIRILVENEKISDNDAVMLCRDIVKKIETDLTYPGQIRVTVIR
                     ETRVTDYAK"
     gene            complement(4335..4531)
                     /gene="ssrS"
                     /locus_tag="PLT63_06780"
     ncRNA           complement(4335..4531)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PLT63_06780"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(4541..4819)
                     /locus_tag="PLT63_06785"
     CDS             complement(4541..4819)
                     /locus_tag="PLT63_06785"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HOD98463.1"
                     /translation="MKKRYHIKVMGQDLSVVSDAEEEHVAQVVRYVNDKVEEIGRQAK
                     HFNSLNIAILAALNIADEFFRKKVAEETRYQQLESRSEQLINMINEAI"
     gene            complement(4853..5071)
                     /locus_tag="PLT63_06790"
     CDS             complement(4853..5071)
                     /locus_tag="PLT63_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98464.1"
                     /translation="MEFVKFTELEERIKRILEEKAALKTHCQELEELLKNKDIELEEV
                     NKQLKGLHEEKETVRTKVDSLLELLQDI"
     gene            complement(5128..6255)
                     /gene="dusB"
                     /locus_tag="PLT63_06795"
     CDS             complement(5128..6255)
                     /gene="dusB"
                     /locus_tag="PLT63_06795"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOD98465.1"
                     /translation="MKIDKLDFDVPVFLAPMAGITNLPFRTIVRRCGCQLAFTEMVSV
                     NGLVRGAERTCRYLDGASEDRPLGVQIFGAEAEIMAAGARIATDRGADLIDINMGCPV
                     KKVVRAGAGSALLKDPAKVGRIVSAVRRATSLPLTVKIRAGWRRSVVNAIEIARIAEA
                     EGADAVIVHPRTADQGFSGRADWSIIRAVKDALGIPVIGNGDIRRAADVGRMMTATAC
                     DGVMIGRAALGNPWIFQEIIDGWPVLRQQGSLGEARVDVARMVSASISEDAGDPTVNS
                     ATQESLLVLEQSDVAVNRRLPVSFIERERVIREHLDLERRYTVPKIGVQNFRKHILWY
                     TKGLPGSASFRQRLSELKDVETLLVALGIYFCQLNNALDFS"
     gene            complement(6304..7056)
                     /locus_tag="PLT63_06800"
     CDS             complement(6304..7056)
                     /locus_tag="PLT63_06800"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOD98466.1"
                     /translation="MEDKSIGAVILAAGKGTRMHSDLAKVLHTIDGRPMLAYSVDLAR
                     AVGAERIVAVIGHQAQQVREFCAAPGLLFVEQRPQLGTGHAVLQARKAFAGFAGRVLV
                     LCGDVPLLRTNTIAALIDHHDATGAAVTVMTVVLSDPGNYGRVVKDANGEVMKIVEAR
                     DATQAEKEIREINTGIYCADSGFLFSAVTRIGNCNAQREYYLTDIMEIARRDGVRTQA
                     YVAADAVEVMGINTPEELACARRIVAAWEDVA"
     gene            7168..8502
                     /gene="purD"
                     /locus_tag="PLT63_06805"
     CDS             7168..8502
                     /gene="purD"
                     /locus_tag="PLT63_06805"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOD98467.1"
                     /translation="MPNILLVGNGAREHVLAETLVRSAHRPRLFAYMKANNPGIADLS
                     QEVRMGGYGDTAAIVDLARHREVDFAVIGPEDPLHAGVVDALRTAGIPAVGPSRSLAR
                     LETSKSFTRSLLEKYGIPGNATFRVFSSMTGLEEFLATLPEVVVKPDGLTGGKGVMVQ
                     GDHFHTKEEALPICRQILEEHGRVIVEEKLVGEEFSLQCLCDGKHVVATPPVQDHKRR
                     FVDDKGPNTGGMGSYSCADHLLPFLRREDILAGIEITRQVAAAIRRETGEYYRGIMYG
                     GFMITPTGLRLLEYNARFGDPEAMNILPLLETDFVDLCSAIINGTLDRLAVSFSSRAT
                     VCKYIVPKGYGLPKDHPDYRSTAARIEVGDTGKARLYYSSVDKREDGLYMTTSRAIGV
                     VGIAAELAEAESIAENAVAALKGPVAHRPDIGTATLIDKRVRHMAEIRKDFP"
     gene            8660..10231
                     /gene="purH"
                     /locus_tag="PLT63_06810"
     CDS             8660..10231
                     /gene="purH"
                     /locus_tag="PLT63_06810"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOD98468.1"
                     /translation="MYLNEIKRALISVTEKQGIVEFARELASFGVEILSTGGTAAKLR
                     NSGLAVLEVSDYTGFPEMMDGRLKTLHPKIHGGLLARRDNPNHMEALRSHHISLIDMA
                     IINLYRFEETVAKPGCTLEEAVENIDIGGPTMLRAAAKNYRFVSVVTDPADYDKIIQE
                     MKRTGGKVSAETNFYLAVKTFQLTARYDGAISNYLGRIKPDGSQADFPQTFTFQFARA
                     QDLRYGENPHQKAAFYKESKPFLSAVANARQLQGKELSYNNIMDADAAWQMASDFNLP
                     AAVVIKHANPCGAATATGDDIRTAFEKALKTDPISAFGGIVAMNRPVDAGTAAELTRI
                     FLEVIIAPAFSDEARSILGTKKNVRILEIPVAASSAPQDYDFRRVVGGLLIQERDTDA
                     FDIRQARVVTKRTPTADEYQALDFAWRVVKHVKSNAIVLAAKDQLIGVGAGQMSRVDS
                     VKIARMKANLPTAGSVLASDAFFPFRDGVDMAAEAGVTAIIQPGGSVRDEEAIQAADE
                     YNIAMIFTGRRHFKH"
     gene            10265..10795
                     /gene="purE"
                     /locus_tag="PLT63_06815"
     CDS             10265..10795
                     /gene="purE"
                     /locus_tag="PLT63_06815"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOD98469.1"
                     /translation="MSNQPVPKVSVVMGSDSDFSVLEEATLILNRFGIPYELFLTSAH
                     RSPARTAAFAQRAAERGIEVIIVGAGAAAHLAGVIASATHLPVIGVPIDATALRGLDA
                     LLATVQMPGGIPVATMAIGKAGARNAALYAIRILSLHDVALKNGLLDYIASMSREVEI
                     KHEKMQQAHSSKKQIS"
     gene            10951..11613
                     /locus_tag="PLT63_06820"
     CDS             10951..11613
                     /locus_tag="PLT63_06820"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOD98470.1"
                     /translation="MKIRNNRIIAAAELDKAVGILAAGGVVAFPTETFYGLAADAGNE
                     GAIDQIFTLKGRSFRNPIALIIAAPPDLEALTEGISDNDHKLMAAFWPGPLTLVFRAV
                     PAVSFRLTAGTGKIGVRISSHPIAQALAAGLGRPITATSANLSGHPGCTAADEVSKQL
                     NSDSLTILNGGPTPGGKGSTILDVTVHPPIILREGMISKAAIANILSGFKPDFKKTRL
                     DY"
     gene            11782..>11983
                     /locus_tag="PLT63_06825"
     CDS             11782..>11983
                     /locus_tag="PLT63_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98471.1"
                     /translation="MKKNPISIFVILIFLCSCLSFAVPIAVGSETYQQPTTNLLDQGI
                     SQYREENYEEAIEILTKARQQDK"
BASE COUNT         2756 a         3499 c         2922 g         2806 t
ORIGIN      
        1 gcacgccgcc agcgtttgtt ctgagccagg atcaaactct ccagttttaa tccttgctga
       61 gaattacctc aattttttat ttgtgtttgt ttgggaccca caaatcaatc atcccactat
      121 tcaattgcca aagaacccag ttggaaacga gctaagttaa atagaaaaac tttcctctgt
      181 tgtcaagcct ttttttaacg caactaaaaa aatttcaaca gcgctaagat tgaattgtct
      241 acagagaatt ccctaaaaag tcaagagcta attttgaaga attcattttc ctgtcgaaga
      301 ctgctttaaa gaacagacgc ggcttctact gtggatcgac cgaagagtca agcatttttt
      361 tacagttttt tttgcgttat cttacgacaa taagaaaatg cttctttttt ccctttctta
      421 ggaggacaag accctggtcg tttatatgac tttccgtgat cttttcatcg aaagaaacga
      481 cgggattttc atttatatat gccccgccct gttcgataag ccgccgtgcc tctccacggg
      541 atgcgcagag tcccgtctca tgaaagagtt cgtaggccgg aatgcctttc tccatatccg
      601 cgtgtgactt ttttagcgag ggaatagccg acagatcgtc agtcccgcca tttatcaacg
      661 ttatgcttcg ctttctaggt atggagctgg aaggaaacag ctccgcgtct acaggccggg
      721 cctgaaaagc ccgcaccgag gaccgccagg cttccaacgc cgctgtttcg ccgtgggtga
      781 tcttcgtggc ctcgaaggcg aggacgcttt tggccatgtt cagacgggcg tcggccaggt
      841 ttcgcacctc gcggatttcc tccacaggca gaaaggtgaa gaggcaaaga aaccgctcca
      901 cgtcttcgtc ttccgtattg acccaatact ggtagtactc atatgggaaa gtgagggctg
      961 agtcgagcca gaccgtcccc cgctccgtct tgcccatttt gtgtcccgcg gaggtggtga
     1021 taagcggaaa ggtcaaactg tgagccgcct tgccctccat acgacgaatg agatcgcttc
     1081 ccgccagcat attgccccac tggtcgttgc ctcccatctg gacgatacag tcgtagtttt
     1141 tgaataggta aagaaaatca tatgcttgta gaagcatata gttgaattcg ataaaattca
     1201 gacctttctc gaggcgcatt ttataactct ccgctgccag catcttgttc acactgaaat
     1261 ggcgaccgat gtcccttaaa aattcgatgt aattcagttt tgtcagccaa tcggcattgt
     1321 tgagcagcag ggcccgaccg gcgccgaaat cgagatagcg ggcaagttgc tgctgcaggc
     1381 accgggcatt gtaatctatc ttatcccggg tcaaaatctg tcgcatctcc gtctttccgc
     1441 tgggatcacc gatcaatccc gtaccgccac caaccaaggc gatgggacgg tggccgtgtc
     1501 gctgcatgtg ggccagggcc atgatcggca ccagagaacc gatatggagg ctcgtcgccg
     1561 tgggatcgaa accaatatag caggtaaccc ctggttcatc gaggagcctc ccgatggcct
     1621 cgtcgtccgt tacctgttcc acgaagcccc gttccagaaa cacatcatag acgttcttca
     1681 tttactccat gcctcctgcc aagcatttac atccccatga ccgatcatgt aacctccagc
     1741 tactcctgat gcaacgggag ggaaagcctc tcgatctgca acgctgtccc gctcttcaca
     1801 tctatttcca tgagcaccgc ctgaagccgc acatcaccct tggccacctc aaacttgttg
     1861 ggaagctggg agagaaaacg ttcgagaatg atttccttgc gggtaccgat cacggaatcg
     1921 aaaggcccgg tcatccccac atcggtaatg taagccgtgc cgctgggaag gatccgttca
     1981 tccgccgtct ggacatgggt gtgagtcccg agcaccgccg tgaccttacc atcgagaaac
     2041 cagcccaggg cgatcttttc cgatgtggct tccgcatgga tatcaactat aatcacctgg
     2101 gtcctcatcc ttagtctctc gatctcccgt tctgcggtgc gaaagggaca ttccaaggga
     2161 tgcatgaaga tccgccccat gaggtttacc acccccacat gacggccatt gacggcaggc
     2221 attaccacac tgccccggcc gggagccggg gccggataat tggctggccg taagagggat
     2281 tcatagtcgt ttatgcaatc aaggatctcc ttcttatccc aaacatgatt gcccgtggtc
     2341 aggacatcaa tctgatttcc aaagagttcc tcgataatct ccctggtcaa gccgaaaccc
     2401 gcggccgcat tttccccgtt ggcaatgacg aagtctattt gccggttccg cacgagttcc
     2461 ggaaggagat cgtgcaccgc ccggcgtccg ggtttaccta ctatatcgcc gacaaataaa
     2521 agcctcatcc tttctccagt tcctataacc aaccgcttgg tcggctcatt tcgcgtagtc
     2581 agtaacccgg gtctcccgga tgaccgtaac ccggatctga cctggataag tcagatccgt
     2641 ctcgattttc tttactatat cccggcagag catcacggcg tcattgtcgg aaatcttttc
     2701 gttttccacc aggatgcgga tctcccgtcc ggcctgaatg gcaaagcacc tgtccacccc
     2761 attgaaggaa ttggcaatgg cctccaactc ctccaaacgt ttgacgtacg attcaagcat
     2821 ttcccgtcgc gccccgggcc gggccgccga aagggtatct gccgcctgga ctagaacccc
     2881 cagcagggtg ttcgtccgcc cgtcatcgtg atgagtggcg atggcctgga caatgcgcgg
     2941 cgattcaccg aatcggcggg caaaatcagc gccgatcgtg gcgtgtggac cctcaatttc
     3001 atgatctatg gccttgccga tatcatggag caatcccgcc cgtttggcct ccttgacgtt
     3061 gagtttcaat tccgccgcca tcatccccgt cagatgcgct acctcgatgg aatgctgcag
     3121 gacattctga gagaagctct tccgaaactt cagactgccc aggagcgtga cgatctccgg
     3181 attgacgtcg tggaccccga cgtcaaaaca ggtccgctcc cccgtttctc taatgatcgt
     3241 atccacctcc gtgcgcacct tcttgacgat atcctcgatc cggccgggat ggatccggcc
     3301 gtcggaaatc agacgttcca gggagatcct ggcgacttcc cgccgcacgg gatcgaaact
     3361 ggacagcacc actgcttccg gcgtatcatc cacgatgaga tcgatacctg tcgcggcctc
     3421 gatggcccgg atgtttcttc cctcccggcc gatgatcctg cctttcatct catcactggg
     3481 cagattgacc acggatacgg tattttcggt aacaaattca ccagaataac gctgtatagc
     3541 ataggctata atttcgctgg ccttcttgtc cgccgtcctt ttcgcctctt cctcgatttt
     3601 gcgaaccgtc gtcgctgcct caatgcgcgc ttcgctttcc atggactgaa tcagcatatt
     3661 cttagcatct tcagatgata tgcaagctat tttttgcagt ttttccttct gttcttcaat
     3721 gctccggttc agatgctcat gcttgtcatg aagggtctgt tccttctgct gcaatcctct
     3781 ttcccttatt tcaattgcgt tttccttttg cgtcagggca tccatgcgtt tttcgaggtt
     3841 ttcctcctta gcccgaatgc gccgctcgac ggcatcgagc tccattttgc gatcccgggt
     3901 atctttttca aactccgtct tgaccttcag caggttttcc ttcgcctgga gcagggattc
     3961 cttcttgatg ttttccgctt ccttctttgc ctcctcgaca atacggccag caagactttc
     4021 cgaggcggcc cgcttttttt tcaacaggaa atggttaact ccgagaccgg cgatcagacc
     4081 aaaggcaact gccgccacaa ttattaatat cattatcact gtactcaaat tttgatacct
     4141 ccttatatgt ataccccccc gcatcgggaa tcgctgcgac cccgattacc ggtcaggcct
     4201 tttgcatgtg ctcggaatag aagctctcct gcccggcttt catcgcctag actaaaccga
     4261 aatatcctgc ggaaataagt aaattttata aagggatttg atttgaaagg gtcgctaatt
     4321 gcttctaaag aaagaaaacc cccgcgattg ccgtgttaac cacctttttg aaccgttccc
     4381 aaaagtgggc gcctaaattt gctgcgtcag gcttcctacc ttccgttgtc acggatgcca
     4441 gcagacatgc acacggcgaa caaggacagg ttcccgattc caaggtgttg gatcaaaaaa
     4501 catcagttaa tcacaaacat cgcaggggat gtaaaaagct ctaaattgcc tcgttgatca
     4561 tgttgatcaa ctgctccgat ctgctctcca attgctggta gcgtgtttcc tcggcaacct
     4621 ttttccggaa aaattcatcc gcgatattga gggccgccag aatagctatg ttcaggctgt
     4681 tgaaatgctt tgcttgcctt ccgatttctt caaccttgtc attaacatac cggaccacct
     4741 gcgccacatg ctcttcctct gcatcgctta ccaccgacag atcttgtccc atcaccttga
     4801 tatgataacg ctttttcaac ctgcataact ccaggagagc caattgcggc gcttatatat
     4861 cttgaagcaa ctcgagcaac gaatccacct tggtgcgtac ggtctccttt tcctcgtgca
     4921 atccctttag ttgtttattt acttcttcca gctctatatc tttattttta aggagctctt
     4981 ccaattcctg gcaatgagtt ttcaaggccg ccttttcctc gagaatcctt tttatcctct
     5041 cttccagttc cgtaaatttt acgaattcca ccctgtccct ccaccttatc ccttattatt
     5101 aacgacgatt gaaatatgcc aaaaggctca tgaaaagtca agggcattgt tgagttggca
     5161 aaagtatatc cccaatgcaa ccagcagagt ctcaacgtcc ttaagttcgc ttaggcgctg
     5221 ccggaaggag gcgcttccag gcagaccctt ggtgtaccaa aggatatgtt tacgaaaatt
     5281 ctgtaccccg atcttgggaa cggtataacg gcgttccagg tccagatgct cccgaataac
     5341 ccgctcacgc tcgataaagg agacgggcag gcgccgattt accgcgacat cactttgttc
     5401 caggaccaat agactttctt gggttgcact atttacagtt gggtcaccag catcctcgga
     5461 tatggatgcc gaaaccatcc tagcgacatc gaccctggcc tcccccaggc ttccttgttg
     5521 acgcaatacc ggccaaccat ctataatctc ttggaatatc cagggattac ccagagcagc
     5581 gcgaccgatc atcaccccgt cgcaggccgt tgcggtcatc atgcgcccga catccgcggc
     5641 ccggcggata tcgccgttgc cgattaccgg aatgcccaga gcgtctttaa ccgcccggat
     5701 gatcgaccag tccgcccgac cgctgaaccc ctgatcagcg gtccggggat gaactatgac
     5761 ggcatccgcc ccctccgcct cagcaatccg ggcaatctcg attgcgttga ccaccgagcg
     5821 ccgccacccg gcccggatct taaccgttaa aggcagggat gtggccctgc gtacggcact
     5881 cacaatccta cccacctttg cgggatcctt gagcagagcg gaccctgctc cggcacggac
     5941 tacttttttc accggacagc ccatatttat atcgattaga tcggcacccc tgtccgttgc
     6001 gatacgtgcc cccgccgcca tgatctccgc ctcggccccg aagatttgca cgcccagggg
     6061 acgatcctca gaggcgccgt ccagataccg acaggtccgt tccgctcccc ggaccagacc
     6121 attgacactg accatctccg taaaggccag ctggcaaccg cacctgcgga caattgttcg
     6181 gaaggggaga ttggttattc cggccatcgg cgcgagaaaa acggggacat caaaatccag
     6241 tttgtcaatc ttcattcccg ggcaccctcg tatagtttag ttctttttgc ctgcatctcc
     6301 tgcttaggcc acgtcttccc aggcagcaac aatacgacgg gcacaggcaa gttcttccgg
     6361 ggtattgatg cccatcacct ctacggcgtc cgccgccaca taggcctgtg tccgcactcc
     6421 gtcccgccgg gcgatttcca tgatatctgt taggtagtat tcccgctggg cgttgcaatt
     6481 gccgatccgg gtcacggcag agaagaggaa cccgctgtcg gcacaataga tccccgtatt
     6541 tatctcgcgg atttcctttt ccgcctgcgt ggcatcccgg gcctccacta tcttcattac
     6601 ctccccgttt gcatctttta ccacccgccc gtaattgccg ggatcggaga gtacaaccgt
     6661 cattacggtt acggccgctc cggtcgcatc atgatgatcg atcaacgccg ctatcgtgtt
     6721 ggttctcagc agggggacgt caccgcagag cacaagcact ctccccgcaa agccggcaaa
     6781 tgccttccgg gcctggagga cggcatggcc cgtacccagt tgaggtcgtt gttccacgaa
     6841 taacagacct ggggcagcgc aaaattcccg cacctgctgg gcttgatggc cgatcaccgc
     6901 tacaattcgt tccgccccca ccgccctggc cagatcgaca ctgtatgcca gcatgggccg
     6961 cccgtcgatc gtatggagga ccttcgccaa atcggagtgc atccgtgtcc cttttcccgc
     7021 cgctagaata acggccccta tggatttatc ttccatattt aatcatcttt cctttcacca
     7081 atgtttcatt gactgaacag tcctttttcg atatttaaca cggccgtggg gattcaacaa
     7141 ccgtaaaaga aagggaggaa cgaggacatg cccaacatac ttcttgtagg caacggggcc
     7201 cgggaacacg tcctggcgga gacactggtc cgttctgcac acaggccccg gctgttcgct
     7261 tacatgaaag caaacaatcc cggcattgcg gacctttccc aagaggtgcg gatgggaggg
     7321 tacggtgata cagcggccat cgttgatttg gcccggcatc gagaagtgga tttcgccgtc
     7381 atcggtcctg aagatcccct tcacgccggt gtcgtagatg ccctgagaac ggcgggcatc
     7441 ccggcggtag gaccctcccg atccctcgcc agactggaaa catcaaaatc cttcacccgc
     7501 agtcttttgg agaaatacgg tatccccgga aatgccacgt tccgggtctt cagctccatg
     7561 acagggctgg aggaattcct ggctaccctg ccggaagtgg tggtgaaacc cgacggcctg
     7621 accggaggta aaggggtaat ggttcagggg gaccatttcc ataccaagga ggaagccctg
     7681 ccaatctgcc ggcagatact ggaagaacat ggacgggtta tcgtggaaga aaagctcgtc
     7741 ggggaggaat tttccctcca gtgcctctgc gacggaaaac atgttgttgc cacgcctcct
     7801 gtccaggacc acaaacggcg gttcgtcgac gacaagggac ccaatacggg gggcatgggc
     7861 tcttattcct gcgcggacca tctcctcccc tttctccgtc gggaagacat cctggcgggc
     7921 attgagatta cccgccaggt cgccgcggcc atccgtcggg aaacggggga atactaccgt
     7981 gggatcatgt acggtggctt catgataacc ccgacgggcc ttcgccttct cgaatacaac
     8041 gcccgattcg gtgatccgga agccatgaat atcctcccgc tcctggaaac tgatttcgtg
     8101 gacctctgca gcgccattat caacggcacc ctggatcgac tggcggtatc cttctcttcc
     8161 cgggcaacag tctgtaaata tatcgtcccc aaagggtatg gtctgcccaa ggaccacccc
     8221 gactatcggt cgactgcggc ccggatcgaa gtgggggaca cgggaaaggc ccggctctat
     8281 tattcttccg tagacaaaag ggaagacgga ctgtatatga ccacctcccg ggccatcggc
     8341 gtcgtcggaa tcgccgccga acttgcggaa gcagaatcta tcgccgaaaa tgccgtggcg
     8401 gcgttgaaag gtcccgtagc ccaccggccg gacatcggca cggcgaccct aatcgacaaa
     8461 cgggtccgcc atatggctga aatcaggaaa gattttccat aagatcaaca atatattagt
     8521 tgaataaatg atatcgtttg ccgttctgct tgcagtcggg accgataaca aaataattcc
     8581 tcaaacacct atgcaggtcg ggaggaaaag cactaaattt gaaaggtttt ttaatactct
     8641 aaaaagcagg gaggcaagaa tgtatctgaa cgagatcaag cgggcgctga tcagcgtcac
     8701 cgaaaagcag ggaatagtgg aattcgccag agaattggca tcattcgggg tggaaatcct
     8761 ttccaccggg ggaacggccg ccaaattgcg caacagcggc ctggcggtcc tggaggtttc
     8821 agattatacg ggcttccccg aaatgatgga cggccgcctc aagacccttc atcctaagat
     8881 ccacggcggc cttctggccc gccgcgacaa tcccaaccat atggaagcct tgcgttccca
     8941 tcacatcagc ctgatcgaca tggcgatcat aaatctctac cgcttcgagg aaacggtggc
     9001 caaacccggt tgcaccctgg aagaggcagt ggaaaacatc gacatcggcg gacccaccat
     9061 gctccgtgcg gcggctaaaa attaccgatt cgtatccgtg gtcaccgatc cggcggatta
     9121 cgataaaatt attcaggaaa tgaagcggac gggcggcaag gtgtcggcgg aaacaaattt
     9181 ctacctcgcc gtcaagacct tccagttgac cgcccgttac gacggcgcta tctccaacta
     9241 tctgggccgg atcaaacccg atggatctca ggcggatttt ccccaaacat tcacctttca
     9301 gttcgccagg gcccaggatc ttcgttacgg cgagaacccc catcagaagg cagccttcta
     9361 caaagaaagc aaaccctttc tttccgccgt cgccaacgcc cgccaattgc agggcaaaga
     9421 gctttcctac aacaatataa tggatgccga cgccgcctgg caaatggcgt ccgatttcaa
     9481 tcttcccgcc gccgtggtga taaagcatgc caacccctgc ggggcagcaa ccgcgaccgg
     9541 cgacgacatc cgaacggcct ttgaaaaagc tttgaagacc gatcccattt ccgccttcgg
     9601 cggcatcgtg gccatgaaca ggcctgtgga cgcgggcacc gccgcggagc tgaccaggat
     9661 cttcctcgag gtaatcatcg cacctgcctt tagcgacgag gcgcgttcca tcctgggcac
     9721 taaaaagaat gtccggatcc tggaaatccc cgtggcagcc tcatccgctc ctcaggatta
     9781 cgatttccgc cgggttgtcg gcggattgct catccaggaa agggatacgg atgccttcga
     9841 tatccgccag gcccgagtcg tcaccaaacg cacccctact gcggatgaat accaggctct
     9901 ggatttcgcc tggcgggtag taaaacacgt aaagtccaac gccatcgtcc tagccgccaa
     9961 ggaccagctc atcggcgtgg gggcgggaca gatgagcagg gtggattccg ttaaaatcgc
    10021 tcggatgaag gccaatctcc ccacggccgg ttcggtcctg gcctctgatg ccttcttccc
    10081 cttccgggac ggagtggaca tggcagcgga ggcaggcgtc acggccatca tccagccggg
    10141 aggttccgtc cgggatgaag aggcgatcca agcggcggac gagtacaaca tcgccatgat
    10201 cttcacgggc cggcggcatt tcaagcactg agggtaaaaa agcagcagat ataggaggcc
    10261 acgtatgagc aaccaaccag ttcccaaagt cagtgtggtt atgggcagtg attccgattt
    10321 ttccgtcctg gaagaagcga ccctgatcct gaaccggttc ggcatccctt acgaactctt
    10381 tctgacttcc gcccaccgct cccccgcccg gaccgccgca tttgcccaga gagcggcgga
    10441 gcggggaata gaagtcatca tcgtcggcgc cggcgcggcg gcccatttgg cgggagtcat
    10501 cgcctccgcg acccatttgc ccgtcatcgg cgttccgatc gacgccaccg ccttgcgtgg
    10561 tctcgacgcc ctgctggcca ccgtccaaat gcccggtggc ataccggtgg caacgatggc
    10621 catcggcaag gcgggggcaa ggaacgccgc cctatatgcg atcaggattt tatcactcca
    10681 tgacgtcgct ctgaaaaatg gactgctcga ttacatagca agcatgtccc gggaagtgga
    10741 aataaaacac gaaaaaatgc agcaagcgca tagctcaaaa aaacaaattt cttgatccgg
    10801 gccgcgctga agcacttaga caccaaggct ccaggaaggg agaccgggcc gttgagagca
    10861 tcccttttac caccgtcatc atggccgtaa ggcagtgtac aaaaattacg aacatcaatc
    10921 gtctcaatga tttgatttca caaatatttc atgaaaattc ggaataacag gattatcgcg
    10981 gcagcggaac tcgacaaggc agtaggcatt cttgccgccg gaggcgtggt cgcctttccc
    11041 accgaaacct tttacggcct agccgccgat gcgggcaacg aaggagctat cgatcaaatt
    11101 tttaccctca aaggtcgttc ctttcgtaat cccatcgcgc tgatcatcgc cgcaccgccc
    11161 gatctcgagg ccttgacgga agggatatcc gacaacgatc ataaactaat ggcggctttt
    11221 tggcccggcc ccctgacctt ggtattccgg gcggtgcctg cggtcagctt ccgcttgacc
    11281 gccggcaccg gcaagatcgg tgtccgcatc tccagccacc ccatcgccca ggcgctggcc
    11341 gcaggactgg gccgcccgat taccgccacc agcgccaatc tctctggtca tcccggatgt
    11401 actgccgctg atgaagtcag caagcaactg aattctgatt cgctcactat tttaaatgga
    11461 ggaccaaccc cgggaggaaa aggatcgacc attctggatg ttaccgttca tcctcccatt
    11521 atcctcagag aggggatgat ttccaaagcc gccatcgcaa atatcttgag cggattcaaa
    11581 ccagacttca aaaaaacgcg tcttgactat tgattgatgc ttctctataa tctctataat
    11641 ctattaaaat atgagttagc cgaaattatt atatattata gaataatttc acaaatagcg
    11701 gatattttca tccttcgttg tgcctgcgcc gggcatgggg gttaatcaaa taaacaagct
    11761 aataattcga gcgataaact aatgaaaaag aatcccatca gcatctttgt tatactcata
    11821 tttttatgct cttgcttatc attcgctgtt cccatcgccg taggatctga aacatatcaa
    11881 caacctacca ccaatctact agatcaggga attagccaat acagagagga gaattatgaa
    11941 gaagccattg agatactgac aaaggctcgt cagcaagaca aga
//
LOCUS       DAOSMF010000057        11931 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_6186_length_11931_cov_22.095571, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000057 DAOSMF010000000
VERSION     DAOSMF010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 11931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11931
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6186_length_11931_cov_22.095571"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1932
                     /locus_tag="PLT63_06830"
     CDS             <1..1932
                     /locus_tag="PLT63_06830"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOD98472.1"
                     /translation="CPPSEAAELAKALGIELEEHGFCKTALENPVETSRPGIFVCGAF
                     GGPKDIPETVMEASGAAARASGILASRRGTMISEEEVPLESDLRGLGPRTGVFVCHCG
                     INIGGVVKVPEVVEYCKTLPNVVFATDNLFTCSQDTAVKMGEVIKEQNLTRVVVASCS
                     PRTHEGLFQENCEKAGLNRYLFEMANIRDQNSWVHMHEPDAATEKAKDLVRMAVAKAQ
                     FLKPLKPGQLTVNHAALIIGGGLAGITAALSLADQGFESYIVEKEPVLGGNYRKLYYT
                     LEGLDTRKHLKDLLEKVQASKLIHAYAGATIAKLEGFIGNYKTTITTRDGEEQFEHGV
                     VIVATGGYELETMEYLHGQSPQVVTQRELEKLIAEKDDKALQAGSVTMIQCVGSRIPE
                     RPYCSRYCCSEAMKNALKLKEMDPAREVTVIYRDIRTFGLKEDYYKKARELNVRFIRY
                     DENRKPEVIQQGDKLSIRVFDPIMNGPMEFKTDLLALSVGTVPNPENVAIGNMLKVPT
                     NQDGFFLEAHVKLRPVDFATDGVFMCGMAHAPKLSEDAIVQANAAVSRACTILTKDFI
                     EAEGKTAFVNKERCSACGLCEENCPFGAIRVNVVEGCAEVNAILCKGCGVCTASCRMN
                     AVDLNGFTNEEELEQIFNI"
     gene            1981..2418
                     /locus_tag="PLT63_06835"
     CDS             1981..2418
                     /locus_tag="PLT63_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:2197..2199,aa:Sec)
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HOD98473.1"
                     /translation="MGENAANKEWVPKIVAIVCNWCTYAGADLAGISRIQYPPNVRII
                     RVPCTGRINPFYVVKALQTGADGVLVSGUHPGECHYISGNLVARRKFATLKSFLDYIG
                     IEGDRTIFTWVSASEGERFANVIKGVTTKVQALGPATKLVKEL"
     gene            2506..3516
                     /locus_tag="PLT63_06840"
     CDS             2506..3516
                     /locus_tag="PLT63_06840"
                     /inference="COORDINATES: protein motif:HMM:NF016328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOD98474.1"
                     /translation="MENIEKKLREEAKRLLEEKRVDALVGYEAGTLPLTATPCFITNP
                     EEADRLVWSAFCTQNLAKYVHDLISQHRNAQKRVKPEDRKKKVVGVVARGCTTRSIVL
                     HLQERQYGRDEVVIIGVPCTGYVDRKGLTSALDGADIQEGSLAGDRLTIQTTGGKKEV
                     SLQDVLADNCRTCKFNNPIICDVMIGEKAPEMPGAAREYAEVEEFEKLSTEERWEYFT
                     KEMSKCIRCYACRQACPSCYCPTCFVEQSQPQWVGLGEDATDTHVFQLMRLFHMVGRC
                     VDCGSCVSVCPMGVDLRKFLKKLDKDAFDFFGNRAGASLEDPPPLSTFKEHDKEDFIY
                     NP"
     gene            3582..4127
                     /locus_tag="PLT63_06845"
     CDS             3582..4127
                     /locus_tag="PLT63_06845"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOD98475.1"
                     /translation="MVNGVNMKTFLEEVNEKINGVPLQRCYHCRKCTAGCPLAFAMEH
                     NPNKVIRMIQMGMKKEVLGSSTIWLCASCETCITRCPNEVDIARMMDVLREISIAAGI
                     GAREKNVLSFHEAFLASIKSRGRINEPFMMVDYKLRSGDLFSDMGMGISMFMKGKLSL
                     VSPKTRDMNTIRKIFDKTKHA"
     gene            4157..5005
                     /locus_tag="PLT63_06850"
     CDS             4157..5005
                     /locus_tag="PLT63_06850"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HOD98476.1"
                     /translation="MKVSYYPGCSLHGTAKEYDQSVKTVSRALGVELKEIEDWSCCGA
                     TSGHATNFKLSVALPARNLMIAKGEAKEMMVPCAACFNRFKSAQYHLRHDEALKKEVE
                     EIVGQKYTPGLDVKSPIDILYNDIGLDALKAKVVKKLTGLRPVSYYGCLLVRPPEVCN
                     FEDYENPYMLDGILATLGADVKNWSYKTDCCGGSLTISRTDIVGKLVDKLMTMAREAG
                     ANCVVTACPVCMANLDARASENLRLPVFYFTELVALALGLPGTSTWFKLHNVDPLPLV
                     SSLGLL"
     gene            5042..6064
                     /locus_tag="PLT63_06855"
     CDS             5042..6064
                     /locus_tag="PLT63_06855"
                     /inference="COORDINATES: protein motif:HMM:NF028488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOD98477.1"
                     /translation="MEKRLIKKDALTGIIGKIAQEAVVFAPVKVEDNVLFKALEKGEE
                     PYLAYANSKNAPKNFFFPRSEVMLRYTRTTKGVEFTGGQKEAETTVLFGVRPCDARSF
                     VLLDLLFDQEKYKDPYYINKRQKTTIVAMACVHPPYATCFCTSVDGHPVSSEGADILL
                     SDLGDQFLAEFVTPKGEQLVKHFGDVKADATAEAKKEAAAEQAAKEIKSHLPGREVKP
                     IMDANFNSPFWNTVHQKCLACGTCTYLCPTCHCFDISDEMKQSDGRRLRNWDSCMFPM
                     FTKETSGHNPRPTQKERWRQRAMHKFSYYPENFNAIACVGCGRCVMYCPVNIDIRKII
                     TDISKL"
     gene            6080..6919
                     /locus_tag="PLT63_06860"
     CDS             6080..6919
                     /locus_tag="PLT63_06860"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HOD98478.1"
                     /translation="MQNPYIPYPVEVIKIVTEVDTKDIKTFRFAFVNKEDEEKFKYIP
                     GQFAELSIFGKGESPIGIASSPTQPGYVEFTVQKAGVVTSALHEMEVGTRMGLRGPLG
                     NSWPIEYLEGKNVVIVGGGFAFTTLRSLINYMIFGDNRKRFGKITVVYGARNPGLLIY
                     KDELEAWSKRDDIDLNVTVDKGDAGWKGREGFVPTVCKEVGPSPENAVTVICGPPIMI
                     RFTLPVFFELGFSKETIITSLEMRMKCGIGKCGRCNVGSKYVCKDGPVFTFAELDKLT
                     RDF"
     gene            7014..7850
                     /locus_tag="PLT63_06865"
     CDS             7014..7850
                     /locus_tag="PLT63_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HOD98479.1"
                     /translation="MSRIVYKEQLSESVVRMDLEAPEIARKRRAGQFLVLKVNERGER
                     IPLTIVDSDGEKGTVTIIYQIVGKTTALMATLAVGDDIQDVQGPLGNPTEIENYGHVV
                     CIGGGVGVGVIYPITKALQEAGNRVTSIIGARNKSLIILEEEMRAASDKLVVATDDGS
                     YGVHGFVSNVLQGLIDAGETIDRVFAIGPVPMMKVIANLTRPYGIKTIVSLNAIMVDA
                     TGMCGACRVAVGGQTKFTCVDGPEFDGHQVDFDLLTSRLRMYCEQEGVAYEQHRCGWQ
                     GN"
     gene            8070..9494
                     /gene="gltA"
                     /locus_tag="PLT63_06870"
     CDS             8070..9494
                     /gene="gltA"
                     /locus_tag="PLT63_06870"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HOD98480.1"
                     /translation="MEEKTEKPRKEKIPRQAMPEQDPGERIRNFEEVPRGYAPETAIL
                     EAKRCIQCKKPGCVEGCPVDVQIPKFIKQIAEGDFLGAARTLKETNSLPAVCGRVCPQ
                     EDQCEKTCILGKKGEPVAIGRLERFAADYERNGGAVAIPEIAPSNGKKVAVIGAGPSG
                     LTIAGDLVKLGYDITVFEALHKAGGVLVYGIPEFRLPKAIVEAEVDYLRKLGVKIVVN
                     AVVGRLKTIDELFAAGFDAVYVAVGAGAPVFMNIPGENLSGIYSANEYLTRSNLMKAY
                     RFPEYDTPIVRGRNVAVIGGGNVAMDSVRTALRLGAENAYIIYRRSEVEMPARKEEVH
                     HAKEEGVQFHLLTNPVAYLGEGGWVSGVKCLRMELGEPDASGRRSPVPVAGSEFVIEV
                     DTVVVAVGTMANPIVPATTPGLETNRRGYIITKGESGETSRPGVYAGGDIVTGSATVI
                     LAMGAGRKAAQAIHRRLSGDDPES"
     gene            9936..10574
                     /locus_tag="PLT63_06875"
     CDS             9936..10574
                     /locus_tag="PLT63_06875"
                     /inference="COORDINATES: protein motif:HMM:NF014997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HOD98481.1"
                     /translation="MQMPTQYTLTEELYHSITHGIGVVLAIAGLAVLVLPALQQDDAW
                     RVISFSIYGGSLTLLYLASTLYHSFRDEQIKLFFRHFDHLSIFLLIAGTYTPVVLICL
                     RGIWGWTLFGLIWAVAVGGMIYELLFLGRYRWITVTIYLGMGWLAVIAVKPMITMVPL
                     GLLWWLLAGGLFYTGGVVFYVRKQMRYHHVLWHIFVLFGSACHFLGFLFFLT"
     gene            10710..11927
                     /locus_tag="PLT63_06880"
     CDS             10710..11927
                     /locus_tag="PLT63_06880"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOD98482.1"
                     /translation="MMKRLNVVVFTCLAILVLVLQAGLAVGDNSPMNLRFLTEQYPPY
                     NFQENGLLKGISVDLLDRISSKQGMNLKIQEIAVLPWTEGYRQVLKDRNKVLFSMTRT
                     PEREKMFQWVGPISPTRVVLFTRKDATRRMTDRWETQKLRIGAIRDDIGHQLALKEGK
                     ANWRLVLRPRSQDLVHLLEEGAIDAWAYEETAGRWFIKHAAANPGDIEAARVLFEGEL
                     YYAFHREASPVIIAAFQQTLDELKNEKDADGSSAYEKIFDRYLKPRYVKDQITAEQVI
                     KLVSLTAAALRADATATLKEINAGRHPYRDRENPALYVFAYDQDINMVAHGDNKMMVG
                     KNFRGKTDVEGKSFRDEIVKKALSKGSGWEDYIYTSPKQSGLYFKTTYYQLATGSDGG
                     KYIVCAGKFKDQP"
BASE COUNT         2696 a         3322 c         3541 g         2372 t
ORIGIN      
        1 tgcccgcctt ccgaggcggc ggaactggcg aaggccctgg gcatcgagct ggaagaacac
       61 ggcttctgta aaaccgccct ggaaaatccc gtggagacat cccggccggg catctttgtg
      121 tgcggcgcct tcggcggccc caaggacatc cccgagacgg tcatggaggc cagcggcgcg
      181 gcggcccgag cgtcgggcat cctggcatcc cggcggggga ccatgatctc cgaggaggaa
      241 gtccctctgg aatcggatct ccgggggctg ggcccccgga cgggtgtttt cgtctgtcac
      301 tgcggcatca acatcggcgg cgtcgtcaaa gtgcccgagg tggtcgaata ctgcaagacc
      361 ctccccaacg tggtctttgc cacggacaac ctctttacct gctcccagga tacggcggtg
      421 aaaatgggcg aggtgatcaa ggaacagaac ctgacccggg ttgtcgtggc ttcctgctcc
      481 ccccggaccc acgaaggact cttccaggag aactgcgaaa aagcgggact caatcgttat
      541 ctcttcgaga tggccaacat tcgcgatcag aactcttggg tgcatatgca cgagcccgat
      601 gcggccacgg aaaaggccaa ggatcttgtg aggatggctg tcgccaaggc gcagttcctc
      661 aaaccgctga agccgggcca gttgacggtc aatcatgccg ctctcatcat cggcggcggc
      721 ctggcgggga ttacggctgc cctctccttg gccgatcagg gcttcgagtc ctatatcgtg
      781 gaaaaggagc cggttctggg cggcaattac cggaagctct attacaccct ggaggggctg
      841 gatacccgca agcatctgaa ggatctactg gaaaaggtgc aggccagcaa actaatccat
      901 gcctatgccg gggcgacgat cgcgaagcta gaaggtttca tcggcaatta caagactacc
      961 atcacgacgc gggacggaga agagcagttc gagcacggtg tggtcattgt agccacgggc
     1021 ggttatgaac tggagacgat ggaatatctc catggccagt ccccccaggt cgtcacgcag
     1081 cgggagctgg agaaactcat tgccgagaag gacgacaagg ctctccaagc agggagcgtg
     1141 accatgatcc agtgcgtggg ctcccggata cccgagcggc cctattgcag ccgttattgc
     1201 tgttccgagg cgatgaagaa cgccctgaag ctcaaggaaa tggacccggc ccgggaagtc
     1261 acggtgatct atcgcgatat ccgcaccttc ggcctgaagg aggattacta taaaaaggcc
     1321 cgggaactga acgtccggtt catccgctac gatgaaaacc ggaaaccgga agtgattcag
     1381 cagggcgaca aactctccat ccgggtcttc gatcccatca tgaacggtcc catggaattc
     1441 aaaacggatc tgctggctct tagcgtgggc accgtgccca atccggagaa cgtggccatc
     1501 ggcaatatgc tcaaggtgcc cacgaatcag gacgggttct tcctggaggc ccatgtgaag
     1561 ctccgccccg tggattttgc caccgacggc gtcttcatgt gcggcatggc ccacgccccg
     1621 aagctcagtg aggacgccat cgtccaggcc aatgccgcgg tttcccgggc ttgcaccatc
     1681 ctcaccaagg atttcatcga ggcggaaggc aagacagctt tcgtgaacaa ggagcgctgc
     1741 tccgcctgcg gcctctgcga ggagaactgc cccttcggcg ccattcgggt gaatgtggtg
     1801 gaaggatgcg ccgaggtcaa cgccatcctc tgcaagggat gcggggtctg taccgcctcg
     1861 tgccggatga atgccgtgga tctcaacggc ttcaccaacg aagaagagct ggagcagata
     1921 tttaatatct aaactaaggt caacggccga gaaggcgcat ggccacggga aggagaatat
     1981 atgggtgaaa atgcagcaaa caaggaatgg gtgccaaaaa tcgtcgccat cgtctgcaac
     2041 tggtgtacct acgcgggcgc cgatctggcc ggcatcagca ggattcagta tccgccgaac
     2101 gtgcgcatca ttcgcgtgcc ctgcacggga aggatcaacc cgttttacgt tgtgaaagcc
     2161 ctgcagacgg gagccgacgg cgttctggtt tccggctgac accccggtga atgccactat
     2221 atttcgggaa atctggtggc gcgccgcaag tttgcgacgt tgaaaagttt cctggactac
     2281 atagggatcg aaggtgaccg gaccatcttt acctgggtct cggcatcgga gggggagcgg
     2341 tttgccaatg tcatcaaagg cgtgaccacg aaggtacagg cccttggtcc ggcgacgaag
     2401 ctggtgaagg agttgtaaag accgttttgg gggtgccggg cgcggcggaa aagccctgca
     2461 tttgcttttg catgaagaca atatcaattc gaggattcat ataaagtgga aaacatcgag
     2521 aaaaagttaa gggaagaggc aaaacggctt ctggaagaaa aaagggtgga tgccctggtg
     2581 ggttacgagg cgggcaccct gccccttacg gcgacgccct gtttcatcac gaatccggaa
     2641 gaggccgacc ggcttgtctg gagcgccttt tgtacccaga acctggccaa atacgttcac
     2701 gatctgatct cccagcatcg taatgcccag aaacgggtca agccggaaga ccggaaaaaa
     2761 aaggtggtcg gtgtcgtggc ccggggctgc accacccgct ccatcgtgct ccaccttcag
     2821 gagcgccagt acggacggga cgaggtggtg atcatcggcg tgccctgcac cggctacgtg
     2881 gatcgcaagg gcctgacgtc ggccctggac ggcgcggata tccaggaggg gtcgttggcg
     2941 ggagaccggc ttaccatcca gacgacgggg gggaagaagg aggtttctct gcaggacgtc
     3001 ctggcggaca attgccgcac ctgcaagttc aacaatccca ttatctgcga tgtgatgatc
     3061 ggcgaaaagg ccccggagat gcccggagcg gcccgggagt acgccgaggt cgaggaattc
     3121 gagaaacttt ccacggagga gcgctgggag tattttacca aagagatgtc caagtgcatc
     3181 cgttgctatg cctgccgcca ggcctgtccc tcgtgctact gccctacctg ctttgtcgag
     3241 cagagccagc cccagtgggt gggcctgggg gaagacgcta cggacaccca tgtcttccag
     3301 ttgatgcgcc tcttccacat ggtggggcgc tgtgtggact gcggttcctg cgtctccgta
     3361 tgtcccatgg gcgtcgatct gaggaaattc ctcaagaaac tcgacaagga cgcctttgat
     3421 ttcttcggca accgggcggg ggcttctctg gaagatccac cgcccctttc caccttcaag
     3481 gaacacgaca aggaagattt tatctataac ccctaaatat gaccggcaac aagatcctgg
     3541 tcaaatcctt cggctgtctg cctttaaccg aggatgaacg gatggtgaat ggagtcaaca
     3601 tgaagacctt tctggaagaa gtaaacgaga aaataaacgg cgtgcccctg cagcggtgct
     3661 atcactgccg gaagtgcacc gccggctgtc ccctggcctt cgccatggaa cacaacccca
     3721 acaaggtcat caggatgatc cagatgggaa tgaaaaagga ggtcctgggt agttccacca
     3781 tctggctctg tgcatcctgc gaaacctgca tcacccgctg tcccaacgag gtggacatcg
     3841 cccggatgat ggatgttctc cgggagatat ccattgccgc cggcatcggg gccagggaaa
     3901 agaatgtcct ttccttccac gaggcgttcc tggccagcat caagtcccgg ggccggatca
     3961 acgagccgtt catgatggtg gactacaagc tcagatccgg ggatctattc tccgatatgg
     4021 gcatgggcat ctccatgttc atgaagggca aattgtccct cgtctctcca aagacccggg
     4081 acatgaatac gatccggaag atattcgata aaacgaagca cgcgtaacgc ggcaagcgac
     4141 agcaaggagg caagtgttga aagtttctta ttatccggga tgttccctcc acgggacggc
     4201 gaaggaatac gaccagtccg tcaagacggt gagccgcgcc ttgggcgtgg aactcaagga
     4261 aatcgaagat tggtcctgct gcggcgccac ctcgggccat gctacgaact tcaagctttc
     4321 cgtggccctt cccgcccgga atctcatgat cgccaagggg gaggccaagg agatgatggt
     4381 gccctgcgcg gcctgtttca atcgcttcaa atcggctcag taccatctcc ggcatgacga
     4441 ggccctcaaa aaggaagtgg aagagattgt cggccagaaa tacacaccgg gattggacgt
     4501 caagagcccc atcgacatcc tctacaacga tatcggcctg gacgccctca aggccaaggt
     4561 ggtcaagaag cttaccgggt tgcggcccgt gtcctactac ggctgcctcc tggtccgtcc
     4621 cccggaggtc tgcaatttcg aagattacga aaatccctac atgctcgacg gcatcctggc
     4681 gactctgggg gcggacgtga aaaactggtc ctacaagacg gattgctgcg gcggcagcct
     4741 caccatcagc cggacggata tcgtcgggaa actggtggac aagctcatga ccatggcccg
     4801 ggaggccggg gcgaactgtg tggtcaccgc ctgtccggtg tgcatggcca atctcgacgc
     4861 ccgggccagc gagaacctgc gcttgcccgt cttctatttt acggagctgg tggccttggc
     4921 gctcggcctg cccggaacct cgacgtggtt caaactgcac aatgtggatc ccctgccgct
     4981 ggtcagcagc cttgggctcc tttaacaaca atatccaata tttctgatac tgaaggacga
     5041 tatggaaaaa cggttgatca aaaaagacgc tctgacaggg atcatcggga agatcgctca
     5101 agaggcagta gtttttgccc cggtgaaggt ggaagacaat gtcctcttca aggcgctgga
     5161 aaagggggag gaaccttacc tggcttatgc caacagcaaa aacgccccga agaacttctt
     5221 cttcccccgc tccgaggtca tgctgcgcta cacccggacg accaaagggg tggagtttac
     5281 cggcgggcaa aaggaggcgg agacgacggt tctgttcggg gtccggccct gcgacgcccg
     5341 gagcttcgtt cttctcgatc tcctcttcga tcaggaaaag tacaaggatc cctattatat
     5401 aaataaaagg caaaagacca caatcgttgc tatggcctgc gtccatcccc cctatgcgac
     5461 ctgcttctgc acctccgtgg acggtcatcc ggtctcttcg gaaggggcgg acatcctcct
     5521 ctccgacctg ggagatcagt tcctggccga gttcgtcacc cccaagggcg aacagcttgt
     5581 caagcatttc ggggacgtca aggccgacgc caccgccgag gcgaagaagg aagccgcggc
     5641 cgaacaggcg gccaaggaga tcaaatccca cctccccgga cgggaagtga agcccatcat
     5701 ggatgccaac ttcaacagtc ccttctggaa caccgtccac cagaaatgcc tggcctgcgg
     5761 gacctgcacc tacctctgcc cgacctgcca ctgcttcgac atctccgacg agatgaagca
     5821 gagtgacggc cggcgcctgc ggaactggga ttcctgcatg tttcccatgt ttaccaagga
     5881 gacttcgggg cataaccccc gtccgaccca gaaagaacgg tggcgccagc gggccatgca
     5941 caagttcagc tactacccgg agaatttcaa cgccatcgcc tgcgtgggtt gcggccgttg
     6001 cgtcatgtac tgccccgtca atatcgacat ccggaagatc attaccgata tatcgaaact
     6061 atagagctgg gagaacgtca tgcaaaaccc ttacataccc tatcctgtgg aggtaataaa
     6121 gattgtcacc gaggtggata cgaaggacat caagaccttc cgtttcgcct tcgtgaacaa
     6181 ggaggacgag gagaagttca agtatatccc cggccagttt gcggagttgt ccatcttcgg
     6241 caagggagag tcgcccatcg gcatcgcctc gtcacccacc cagcccggct acgtggagtt
     6301 tacggtccaa aaggcgggcg tcgtcacctc ggccctccat gagatggaag tggggacgcg
     6361 gatgggcctc cggggtcctt tgggaaactc ctggcccatc gagtacctcg aagggaagaa
     6421 cgtggtcatt gtgggcggcg ggttcgcctt caccaccctg cgctccctga tcaactacat
     6481 gatcttcggt gacaaccgga agcgtttcgg caagatcacc gtggtttacg gcgcccggaa
     6541 tccggggctc ctgatctaca aggacgagct ggaggcctgg agcaaacgcg acgacatcga
     6601 tctgaacgtc accgtggaca agggcgacgc cggctggaag ggccgggaag gctttgtgcc
     6661 cacggtctgc aaggaggtcg gccccagccc ggaaaacgcc gtgacggtta tctgcggccc
     6721 ccccatcatg atccgcttca ccctcccggt cttcttcgag ctgggctttt ccaaggaaac
     6781 catcatcacc tccctggaga tgcggatgaa atgcggcatc ggcaagtgcg gccgctgcaa
     6841 cgtggggagc aagtacgtct gcaaggacgg ccccgtgttc accttcgccg aactggataa
     6901 actgacgagg gacttttgaa gacttcgtaa gcagttcaca taccccataa gtgatcagaa
     6961 ggagccccgc cggctccttc tgcgcatcta agcaaagaaa aaggagtata ttcttgagca
     7021 ggatcgttta caaggaacaa ctatcggaaa gcgtggtccg gatggatctg gaggccccgg
     7081 agatcgcccg gaagcgccgg gcgggccagt ttctcgttct caaggtgaac gagcggggag
     7141 agcggatacc tctgaccatc gtggattccg acggggaaaa ggggaccgtg acgatcatct
     7201 accagatcgt cggaaagacc acggccctca tggccacgct cgccgtgggg gacgacattc
     7261 aggacgtcca gggccccttg ggcaatccca cggagatcga gaattacggc cacgtagtct
     7321 gtatcggcgg cggcgtgggc gtgggggtca tctatcccat caccaaggcc ctccaggaag
     7381 cgggaaaccg ggtgacctcc atcatcggcg cccggaacaa gtccctgatc atcctggagg
     7441 aggagatgcg ggcggcgagc gacaaactcg tcgtggccac cgacgacggc agctatgggg
     7501 ttcacgggtt cgtaagcaat gtcctccagg gtctcattga tgccggggag acgatcgacc
     7561 gggtcttcgc catcgggccc gtgcccatga tgaaggtcat cgccaacctc acccggccct
     7621 acggtatcaa gaccatcgtc agcctcaacg ccatcatggt ggatgccacg gggatgtgcg
     7681 gggcctgccg ggtcgccgtc ggggggcaga cgaaattcac ctgtgtcgat ggaccggaat
     7741 tcgacggcca tcaggtggac ttcgatctcc tgacgagccg cctgcggatg tactgcgagc
     7801 aggagggagt ggcctacgaa cagcaccggt gcggctggca gggcaactga gaatgatggc
     7861 gccatgttcc cctttgggac ctgagacgtt gggattgttg cttcacggca ggccttacgt
     7921 ctttcccgca ccggtgggaa gccggtgttt tcgaatactt tccgggtgac ggctcaaccc
     7981 cggcatgact aacggcctcg ctttacgagg atctcacccg gtcttacgcc gggggtaatg
     8041 atatttatca gcggcgatat gaggaaataa tggaagaaaa gacagagaaa cccaggaaag
     8101 aaaagatccc ccgccaggcc atgccggagc aggatcccgg ggagcggata aggaatttcg
     8161 aggaagtgcc ccggggatat gcaccggaga cggcgatcct ggaggcgaaa cgctgcatcc
     8221 agtgcaagaa gccgggttgc gtggagggct gccccgtgga tgtccagatt cccaagttca
     8281 tcaagcagat tgccgaaggc gattttctgg gcgcggcccg aaccctgaag gaaaccaaca
     8341 gcctcccggc ggtttgcggc cgtgtctgtc cccaggagga ccagtgcgag aagacctgca
     8401 tcctcgggaa gaaaggggag ccggtggcca tcggtcgtct ggagcggttc gccgcggatt
     8461 acgagcggaa cggcggggcc gtcgccattc cggagattgc cccgtccaat gggaagaagg
     8521 tggcggtgat cggggcgggt ccctcggggc tcaccatcgc cggcgatctc gtcaagctgg
     8581 gctatgatat cacggtcttt gaggccctcc acaaggcggg aggtgtcctg gtttacggta
     8641 tccctgaatt ccgccttccc aaggccatcg tggaggccga ggtagactat ctccgcaaac
     8701 tgggggtcaa gatcgtcgtc aacgccgtag tcggccgcct gaagaccatc gacgagctgt
     8761 tcgccgcggg attcgacgcc gtctatgtgg cggtcggcgc cggagcgcca gtgttcatga
     8821 acatccccgg tgaaaacttg agcggcatct attccgccaa tgaatatctc acccggtcga
     8881 atcttatgaa ggcctaccgg tttcccgaat acgacacgcc catcgtccgg ggcaggaacg
     8941 tggccgtcat cggcggtggt aatgtggcca tggactcggt gcggacggct ctgcgcctgg
     9001 gggcggaaaa cgcctacatc atctaccggc gcagcgaggt ggagatgccc gcccggaagg
     9061 aagaggttca tcacgccaag gaggaggggg tgcagttcca tcttttgacc aatcctgtgg
     9121 cgtatctggg agagggcggc tgggtgagcg gtgtcaaatg cctccgaatg gagctggggg
     9181 aaccggacgc ctcgggccgg cggagccctg tccccgtggc cggatcggaa tttgtcatcg
     9241 aagtggatac cgtggtggtc gccgtgggca ccatggccaa tcccatcgtc cccgctacca
     9301 ctccggggtt ggagaccaac cgccggggct atatcattac caaaggtgag tcgggagaga
     9361 cgagccgtcc gggggtctat gccgggggtg acatcgtcac cggttccgcc acggtgatcc
     9421 tcgccatggg ggcgggacgc aaggccgccc aggccatcca ccgccgtctt tccggagatg
     9481 acccggaatc ctgatcccgc tgcgggaagg caagatcaat ctggagatgc ggatgcgcac
     9541 ctttcccggt catgcccggg acgcggaggc caatcgagta atggcctggg acaggaaggg
     9601 acatatcccg atcctggaga tgacagcaaa catcactccg atctttccac ctatatttgg
     9661 cttcccacct gtctccggga ggaacctgcc gctgctggga aacagctcgg gagaaatgaa
     9721 caaaaacggg gagcaatgtc gttaaatcct ttattatatc aattggttaa tatatgccgg
     9781 agcaccggcg aagtttattc atcggcaaac aacgacgcat attttatcaa caatagcgtt
     9841 atgatgttgg tcaggggtga gatgaatatg gatgacatcg tgcaaattgt tctgatgttt
     9901 tgctctttta cttgctcttg tggtatggcg catacatgca gatgccaacg caatacaccc
     9961 tgaccgagga attataccac agcatcaccc atggcattgg tgtggtactc gccatagcgg
    10021 gcctcgccgt gctcgttctt cccgccctgc agcaggacga cgcatggcgt gtcatcagtt
    10081 tttccatata tggaggatct cttaccctcc tctacttggc ctcgacactc taccacagtt
    10141 ttcgtgatga gcagatcaag ctttttttcc gccacttcga ccatctttca atttttcttc
    10201 ttattgccgg gacttatacg cccgtagtcc tcatctgcct gcgcggaata tggggttgga
    10261 cgcttttcgg ccttatctgg gccgtcgccg tgggtggcat gatctatgag ttgctgttcc
    10321 tcggccgtta tagatggata acggtaacga tctatctggg gatgggatgg ttggccgtga
    10381 tcgcggtaaa accaatgatc acgatggttc ccctgggcct cctatggtgg ttgctggcgg
    10441 gtggtctctt ctacaccggc ggcgttgttt tctatgtccg taagcagatg cggtatcacc
    10501 atgtgctgtg gcacatcttt gtccttttcg gcagcgcttg tcacttcctt ggatttctgt
    10561 ttttcctgac ttaaaagtat atgcggatga agcaatcttc ccctaaggat aatgaattcg
    10621 taaaaaaacc ctcatgcccc gtggagcacc acgaagcatg aaaatggcat tttcatataa
    10681 aggcggaata acttcaggga ggggaaatta tgatgaaaag attgaacgtt gttgtattta
    10741 catgtctcgc aatcctcgta ttggttctcc aggccggcct cgccgtcggc gataattcac
    10801 caatgaactt gagatttctg acggaacaat atccacctta caatttccag gaaaacggcc
    10861 tcctcaaggg gatctccgtc gatctcctcg accggatctc ctccaagcag ggaatgaatc
    10921 tcaagataca ggagatagcg gttctccctt ggacggaggg ctaccggcaa gtcctgaagg
    10981 accggaacaa ggtccttttc tccatgacga gaacccctga acgggaaaag atgtttcaat
    11041 gggtcggtcc catctccccg accagggtcg tcctcttcac ccgcaaggat gcgacgcgcc
    11101 gcatgactga caggtgggaa acccaaaaat tgaggatcgg cgcgatccgt gatgacatcg
    11161 gacatcaact ggcattgaaa gaaggaaaag caaattggcg actggtcttg aggcctcgct
    11221 cccaggattt ggtgcatctg ctcgaagagg gggcgattga cgcctgggct tacgaagaga
    11281 cggccggacg gtggttcata aaacatgcag ccgccaaccc gggtgatata gaagctgccc
    11341 gggtcctctt tgagggggag ctgtattatg cctttcatcg ggaagcatcg ccggtcataa
    11401 ttgccgcttt ccagcaaacg ctggacgaac tgaaaaacga aaaggacgcc gacggatcga
    11461 gtgcttatga aaagattttt gaccgctatc tgaaaccaag atacgtcaag gatcaaatca
    11521 ccgctgaaca ggtcataaaa cttgtcagtc ttacggccgc cgctttgcgg gctgatgcca
    11581 ccgccacgct gaaggagata aacgccgggc ggcatcctta ccgagacagg gaaaatccgg
    11641 ccttgtacgt ctttgcctat gatcaggaca tcaatatggt cgcccatggc gacaataaaa
    11701 tgatggtggg aaaaaacttc cgcgggaaga cggatgtgga aggcaagtcc tttcgggatg
    11761 aaatcgtaaa gaaggcccta agcaaaggca gcggctggga agattacatc tataccagcc
    11821 ccaagcaaag cggtctctac ttcaagacca cctattatca acttgccacc ggcagtgacg
    11881 gcgggaaata catagtctgt gccggaaaat tcaaggatca accttgacga c
//
LOCUS       DAOSMF010000058        11727 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_6316_length_11727_cov_14.934973, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000058 DAOSMF010000000
VERSION     DAOSMF010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 11727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11727
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6316_length_11727_cov_14.934973"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="PLT63_06885"
     CDS             complement(<1..202)
                     /locus_tag="PLT63_06885"
                     /inference="COORDINATES: protein motif:HMM:NF014398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98483.1"
                     /translation="MAAKNSMGFHDPDQLLNTLGRSIDMAHQAIATTNRAGTSSYDLS
                     GCIIYASICRRIKTMLILRHFSM"
     gene            complement(583..1131)
                     /locus_tag="PLT63_06890"
     CDS             complement(583..1131)
                     /locus_tag="PLT63_06890"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOD98484.1"
                     /translation="MKPTEELAHEHKVIQHVLKTAAAEARSIHDREIVRTALMRMMLD
                     FFRNFTDKCHHAKEEQHLFPMLEKRGMAHDAGPIAVMLAEHAEGRRRLADIAELLPTA
                     GEGDREAVLAIMENLMAYVDLLEGHISKENNVLFPMAERLLGETDMEELEKAFALVEE
                     IETGEGVHEKYHRLAHEIAGYA"
     gene            complement(1128..2336)
                     /locus_tag="PLT63_06895"
     CDS             complement(1128..2336)
                     /locus_tag="PLT63_06895"
                     /inference="COORDINATES: protein motif:HMM:NF008887.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HOD98485.1"
                     /translation="MKIEVTANVDWVGKIDWELRKFHGEEYSTHRGSSYNSYLVRDEK
                     TALIDTVWGPFATEFVQNLAGEIDLDNIDYVIANHAETDHSGALPELMRHIPGKPVYC
                     TANGVKSLRGHYRQDWNFQVVKTGDRLSLGKKEFIFIEAPMLHWPDSMMEYLTGDAVL
                     FSNDAFGQHIAREHIFNDQVGQPELYAEAVKYYANILTPFSPLVTKKINEVVGFNLPL
                     NAICPSHGVIWRDNPLQIVQQYLQWADSYQENQITIVYDTMWNGTRKLAEGIARGIAA
                     SDPDVTVKLFNIARSDKNDVITEIFKAKAVLLGSPTINRGFLSSLALLLEMAAGLKFR
                     NKKAAAFGCYGWSGEAVQLMNEGLIKAGFEVVSPGLKTSWEPGDDELAKAHDFGASLP
                     SLIKTGKEHS"
     gene            complement(2467..2937)
                     /locus_tag="PLT63_06900"
     CDS             complement(2467..2937)
                     /locus_tag="PLT63_06900"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOD98486.1"
                     /translation="MFTASEVVEFAERIHENGANFYQYAVKLVRNQEAKDLFSELAAE
                     EKEHRKVLAGIFAGLEKAIPAETYAGEYAEYLRNYIDNNLIFTKEAMDKDVVSIKDAC
                     SAIDFADRQELESICYYQGIKALVPQVQHYLIDNLISEERKHFVRLTNLRRCVL"
     gene            complement(3025..3735)
                     /locus_tag="PLT63_06905"
     CDS             complement(3025..3735)
                     /locus_tag="PLT63_06905"
                     /inference="COORDINATES: protein motif:HMM:NF012400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b5 domain-containing protein"
                     /protein_id="HOD98487.1"
                     /translation="MKEFDSESLSTFNGKDGQPCYFAHGGRVIDVSGSRLWKTGVHMK
                     RHQAGRDLTLDIKAAPHGPDVLDRYPHVGTFAEETKRHDLPAPLETMLERLPFLRRHP
                     HPMAVHFPMAFSIAPALFYSLYLITMVKSFETTALHCLGASILFLLPAVITGFLTWRI
                     NYQAKTMRAVRIKIRASFILLAVALGSFLLRLQSPKGFAAASWEGVIYFLLLLALVPI
                     VAVIGWHGGSLTFPVEGE"
     gene            complement(3798..4220)
                     /locus_tag="PLT63_06910"
     CDS             complement(3798..4220)
                     /locus_tag="PLT63_06910"
                     /inference="COORDINATES: protein motif:HMM:NF040052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6306 domain-containing protein"
                     /protein_id="HOD98488.1"
                     /translation="MQLRQSQEVISFFNSMLAAERAGVEVLSGLLKEVADKDLKIVLE
                     RFLRDEGMNCQIFSTLIRNTGEDPGKTTGDFVQKVKRLDNIHDKLNLLVKGQEWVAGQ
                     IRKNRPLLVYGSEAVFMESIKIQHEENVDFLKRLLGPA"
     gene            complement(4431..4880)
                     /locus_tag="PLT63_06915"
     CDS             complement(4431..4880)
                     /locus_tag="PLT63_06915"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOD98489.1"
                     /translation="MFAPKNILVPTDFSEYADAALKKAVDIAQQYRGKIYLLHVIDEQ
                     IRQCAVDYCIGSEVVERLETESVKASNEKLQKEVAAVTDARQLEIVFDVQKGNPAEVI
                     LAEQQAKGIDLIVIASHGKTGLIKHLIGSVADKVVKGAKCPVIVIKP"
     gene            complement(4914..5303)
                     /locus_tag="PLT63_06920"
     CDS             complement(4914..5303)
                     /locus_tag="PLT63_06920"
                     /inference="COORDINATES: protein motif:HMM:NF015581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HOD98490.1"
                     /translation="MINYGFTKELAVPCQAVIAQVREALGKEGFGVLAEIDIQRKMKE
                     KLGVDMAGYLILGACNPPYAYQAILAEENIGLMLPCNVAVYEKKNMTVLAIVRPTVAM
                     QMIDNMELKKLAEAIEEKLKAVFDSIK"
     gene            complement(5410..5556)
                     /locus_tag="PLT63_06925"
     CDS             complement(5410..5556)
                     /locus_tag="PLT63_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98491.1"
                     /translation="MKQQSSDQGWEQLQEHIAGFLSQALRSDIQKIEYKPQFTPPLME
                     VPPE"
     gene            complement(5594..6205)
                     /gene="wrbA"
                     /locus_tag="PLT63_06930"
     CDS             complement(5594..6205)
                     /gene="wrbA"
                     /locus_tag="PLT63_06930"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HOD98492.1"
                     /translation="MKVLIVYYSMYGHIHRLGKAVGEGAGSVTGAEVGICRVPETLAE
                     DVLAKMGALEAQKAQAHVPVCQTEDLAASDAIIFGTPTRFGNMCGQMRQFLDGTGGLW
                     SRGALVGKVGSVFTSSATQHGGQESTILSFHLNLLHHGMVIVGLPYTFAGQMRIDEVT
                     GGSPYGASTIAGGDGMRMPSENELAAARYQGTHVAEIAAKLFR"
     gene            complement(6555..8984)
                     /locus_tag="PLT63_06935"
     CDS             complement(6555..8984)
                     /locus_tag="PLT63_06935"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOD98493.1"
                     /translation="MKLADGQRRLQEAEELLQTHIAARQQAEESLQHEQDNLEVRMLE
                     RTAELESARAMAEAAHQRLYGVLETLPVYVALLSEDYRVPFANRFFRSRFGDSQGRHC
                     FEYLFCRTAPCENCESFKVMQTGQPHRWEWLGPDGRTYDIFDFPFTDANGARMIMEVG
                     VDITEHRQAQEELRRHRDNLEILVAERTWELDRSEKRHRSYIEMTGQLGWTTDVNGRV
                     TEDIPAWRRFTGQSKEEVKEAGWTRALHPDDREEAERTWKNAVATGDRYEVEYRLRRY
                     DGVYRTFLARGVPVFKDDGSVLEWVGTCIDITEHKRGEEALRENERRLNHAQRIAHLG
                     SWELDVVNNRLSWSDEVYRIFGAQPQEFTATYETFLAAVHPEDRYAVDAAYSNSVRGG
                     QAGYEIEHRIVRKATGEIRTVQERCNHIRDASGRIVRSVGMVHDITERKEMEEELVKA
                     HDMLEQQVEERTAELRTALTEVKALKDRLEAENIYFRQEIKLHHQHAAILGQSEALRY
                     ALYRAEQVAPTTTTCLILGETGTGKELFAFAIHDMSPRKERSMITVNCAALPANLIES
                     ELFGREKGAFTGADTRQVGRFEVANGSTLCLDEIGEMPLELQAKLLRVIQHHEFERLG
                     SSCTVKVDVRIIATTNRDLEAEVRKGRFRQDLYYRLNVFPITVPPLRQRKDDIPIMVR
                     AFVERLSRKMGKQIDSIPRDMMQVLIDYSWPGNVRELESVIERAVILAPGSVLQLADN
                     LEISPPVLSAATKTLVETERNHILRILADTGWRIEGKEGAASILGLHPSTLRARMHKL
                     GIVRPGRGETD"
     gene            complement(9646..10305)
                     /locus_tag="PLT63_06940"
     CDS             complement(9646..10305)
                     /locus_tag="PLT63_06940"
                     /inference="COORDINATES: protein motif:HMM:NF024929.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HOD98494.1"
                     /translation="MTAAADKSAPPKDGHAASSPDGGPAAAPLGNAPADGPVIVDSRM
                     TFAAAIAGTKAPPEVIAQLCLVDVRYYSFDGRRHQGQLVVHRAVRQDVVEIFQLIDIT
                     RFPIGKAVPIVAYRWSDTASMRDNNTSAFNYRTVAGTKRLSRHALGLAVDINPFRNPV
                     VYDHGRISPRGAVYRPGEPGVLTREHPVVREFLRRGWQWGADFKTMKDYHHFDHPLPG
                     Q"
     gene            complement(10479..11489)
                     /locus_tag="PLT63_06945"
     CDS             complement(10479..11489)
                     /locus_tag="PLT63_06945"
                     /inference="COORDINATES: protein motif:HMM:NF027594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4922 domain-containing protein"
                     /protein_id="HOD98495.1"
                     /translation="MTIIDDDVAPELIWSEFTGMPGEPSLARRCGELFAQQLYNWQEF
                     AAAHMEMNGAWLRELNAAGHTLLVQYNPGRSVSATAAVDASSVRRRPCFLCHYRLPAA
                     QQAVRYRGDFLILVNPRPIFSPHFTVAHVRHLPQRLADHVGTLLTLAKDLGNDFTVFY
                     NGPRCGASVPDHHHFQICPAGAVPVEKEWEAGSRIMNGNRPGVKFGVWNLPGRSAVVM
                     EGPDREALAALFTELIAALGTATDSGEEEPLLNLLATHREGRWRLFLFPRRKHRPDAY
                     YREGDDALLVTPGAVEMGGVIITVREGDFLRMTAPLAVEIYGEVSLHAEEVGEIIDKY
                     LG"
BASE COUNT         2467 a         3387 c         3084 g         2789 t
ORIGIN      
        1 tcatagagaa gtgcctcaat atcaacatcg tttttattct tctgcaaatg cttgcataga
       61 taatacagcc cgataaatca taggaagagg ttcccgcccg attggtggta gcaattgcct
      121 gatgggccat atcgatggag cggccaaggg tgtttagcag ctgatcggga tcatggaacc
      181 ccatgctgtt cttggcggcc acgatatccc aaaaccattg tgcctttcag accaattcct
      241 tcgccttcat aagttctgac ttgttgacat tggcaatggc ccccgccttg ccaataacct
      301 tgtggccctt ggccactgcc tgtcctgccg tcctctgaag ctgccagacg ttgttctggc
      361 ccgacttgac tcgattgagg agacttaatg atggaagagg ctaccttccc ggcggggacc
      421 atttttccgc aatttaaatt tagtggtcct aacatcgatt ctaaaacgaa tcactttttt
      481 aaaaaaattg gaatttcaga acgatcagtg aatggcgtca atagcgacca ttgcagaaat
      541 cataatgatg actcaaagtt cagattagcg agttgttttc tttcatgcat aaccggcgat
      601 ctcatgggca agacgatgat acttttcatg cacgccttct cccgtctcga tctcctcaac
      661 cagggcaaac gccttttcca gttcttccat gtccgtctca ccgagcaatc tttcagccat
      721 gggaaacaag acgttattct ctttggatat gtgcccctca aggagatcca cataagccat
      781 gaggttttcc ataatcgcca gaacggcctc cctgtcgccc tctccagcgg tgggaagcaa
      841 ctcggcgata tcggccagcc tgcgcctgcc ttcggcatgc tccgccagca ttaccgcaat
      901 gggtcccgca tcatgtgcca tacccctttt ttcgagcatg gggaagagat gctgctcttc
      961 tttggcatgg tggcatttat cggtgaagtt cctgaagaag tcgagcatca ttctcatcag
     1021 ggctgtcctg acgatttccc ggtcatggat ggaccgcgcc tccgccgcgg cggttttcaa
     1081 cacgtgttgg atgaccttgt gttcgtgtgc cagttcttct gttggtttca tgagtgttcc
     1141 tttcctgtct tgataaggga gggaagtgac gccccaaagt catgagcctt agccaattcg
     1201 tcatctccgg gttcccagga ggtcttgaga ccgggggaga cgacctcgaa tccggccttg
     1261 ataagccctt cgttcatcag ttgcaccgcc tcgccgctcc atccgtaaca gccgaaggcc
     1321 gccgcttttt tgttccggaa tttcaacccc gcagccatct ccagcaggag cgccagggag
     1381 gacaagaagc ccctgtttat ggtcggtgag ccgagaagga cggccttcgc cttgaagatt
     1441 tccgtgatca cgtcgttctt gtcgctgcgg gcgatattga atagtttgac cgtcacgtcg
     1501 ggatcgcttg ccgcaatccc tctggcgatc ccttccgcca gtttgcgcgt gccgttccac
     1561 atggtgtcgt agacaatggt aatctggttt tcctgataac tgtcggccca ctgtaaatac
     1621 tgctgcacga tctggagggg attgtcacgc cagatgacgc cgtggctggg acagatcgcg
     1681 ttcaggggga ggttgaaacc gacaacctcg ttgatcttct ttgtcaccag cggtgaaaag
     1741 ggggtgagga tgttggcgta gtatttgacg gcctccgcat acagctcggg ttgtcctact
     1801 tggtcgttga agatatgctc tctggcgata tgctgaccga aggcgtcgtt ggagaagagg
     1861 acggcgtccc cagtcaggta ttccatcatg ctgtccggcc agtgtagcat cggcgcttcg
     1921 atgaatatga actccttttt cccgagggac aggcgatcac cggtcttgac gacctggaaa
     1981 ttccagtcct ggcggtagtg tccccggagg gattttacgc cgttggccgt acagtagacc
     2041 ggcttgccgg gtatgtgccg catgagctcg ggcagggctc cggaatggtc cgtctccgcg
     2101 tgattggcga tcacgtaatc gatattgtcc aggtcgatct cgccggctag attttggacg
     2161 aattccgtgg caaaggggcc ccaaacggta tctatcagcg ccgttttctc gtccctcacc
     2221 agataggagt tgtatgacga tccccggtgg gtcgagtatt cctcgccatg gaacttcctc
     2281 aactcccagt cgatttttcc gacccagtcg acatttgccg taacctcgat tttcatagtc
     2341 ggtcctctct ttctatagta tttgttttcc agatttcccc atacggtatt aacggcggaa
     2401 cgtccttgac ttaaatcaat ccggaaggac ggatcacccg aacgcattat cggccgggtt
     2461 tgccggctat aggacgcatc ggcgcagatt ggtcaatctg acgaaatgct tcctttcctc
     2521 actgatcagg ttgtcgatca ggtaatgctg cacctgggga acaagggcct tgatcccctg
     2581 atagtaacag atggattcca gttcctgccg gtcggcgaaa tcgatagcgg aacaagcgtc
     2641 ctttatcgaa accacgtcct tgtccatggc ctcctttgtg aatatgaggt tgttgtcgat
     2701 atagttgcgc agatattcag cgtattcgcc ggcgtacgtc tccgccggta tagctttttc
     2761 caagccggca aaaatcccgg ccaaaacctt ccggtgctcc ttttcctcag ccgcaagctc
     2821 ggaaaacaga tctttcgcct cctggttccg gaccaatttg actgcgtact ggtagaaatt
     2881 cgctccgttc tcgtgaatcc gctcggcgaa ttcaacgact tcactggcag taaaaatatt
     2941 tatgattgcc ctccaaaaac ggtgcctgca aggttgaacg ataaaatata gctattgagc
     3001 cgctgcggct atcccagccg ttcttcattc cccctccacg ggaaaggtca ggcttccccc
     3061 atgccacccg ataacggcaa caatgggaac gagggcaagg aggagcagaa aatagatgac
     3121 gccttcccaa gacgcggcgg caaaaccctt cggagactgg agccgcagga gaaatgatcc
     3181 gagggcaacg gcaagcagga taaaggaggc gcggattttt atccgcaccg cccgcatggt
     3241 tttcgcctga taattgatcc gccaggtcaa gaaaccggtg atgacggcgg gtagaagaaa
     3301 aaggatgctc gctcccaggc agtgcagggc cgtggtctcg aaagatttca ccatcgttat
     3361 aaggtagaga gaatagaaaa gcgccggtgc aatcgagaat gccatgggga aatggacggc
     3421 catcgggtgc ggatgacggc gtaaaaaggg caacctctcc agcatcgttt ccaggggggc
     3481 aggtaaatcg tgccgctttg tttcttccgc aaatgtaccc acatgaggat atctatccag
     3541 cacatccgga ccatggggtg cggccttgat atccaaggtg agatcccttc ccgcctgatg
     3601 gcgcttcatg tgcacccctg ttttccatag cctgctcccg ctgacatcga taacccggcc
     3661 gccatgggcg aagtagcagg gttgtccgtc tttgccgtta aaagttgaca atgattccga
     3721 atcgaattct ttcatgccgg catctcctgg tggatattca agcaaaggga aggtcccttt
     3781 tatctccggc gttccgatca cgccgggccg agcagtcgtt tcagaaaatc cacattttcc
     3841 tcatgctgga tcttgatgga ttccatgaac acggcttcgg acccataaac cagcaagggg
     3901 cggttctttc tgatttgccc ggcaacccat tcctgtccct tgacaagtaa gttcagcttg
     3961 tcatgaatat tatccagcct tttaactttt tgtacaaagt cgcccgttgt tttcccgggg
     4021 tcctcccctg tgtttctgat cagcgtagag aatatctggc agttcatgcc ctcgtccctg
     4081 agaaatcttt ccaagacgat cttcaaatcc ttgtctgcga cctctttcag caatccgctc
     4141 agaacttcca ccccggccct ttctgccgca agcattgaat tgaaaaatga aatgacctcc
     4201 tgactttgtc gcaattgcat cttcatccct cctgacaaaa tattacgcaa gctatggcgc
     4261 tcgattgtca accggcagct tttcctgcgg gtggcgccgt caattcggcg cctcaagggg
     4321 gagatgtccc cgcatcaatg gcgccaggca tcgttggatt cccagaggcg aatcctgctt
     4381 ccggcattcc ccctcttatg gacgccggaa gcagttaaaa tcaggcgggg ctagggtttg
     4441 atcacgatca cgggacattt cgcccctttc acgaccttat ccgccaccga gccaatgaga
     4501 tgctttatga ggcccgtttt gccatgggag gcgatgacga tcagatcgat cccctttgcc
     4561 tgctgttctg caagaattac ctctgccggg ttgcccttct gaacgtcaaa aacgatttcc
     4621 agttgcctgg catccgtgac ggccgcgacc tccttttgca gcttctcgtt cgacgctttg
     4681 acactctccg tttcaagccg ttccacgact tccgatccga tgcagtagtc cacggcgcac
     4741 tgacggatct gttcgtcaat gacatggagc agatagatct tgccccggta ctgctgggca
     4801 atatcgacgg cctttttcaa tgccgcatcc gcatactcgg aaaaatcggt gggaaccagg
     4861 atattcttcg gtgcaaacat agttcttcct ccttccattg tttaatttaa tctctatttt
     4921 atgctgtcaa agacagcttt cagcttttcc tcgatggcct ctgccagctt tttaagttcc
     4981 atgttgtcga tcatctgcat ggcaaccgtg ggccgtacta tggccaggac cgtcatgttt
     5041 ttcttttcgt aaacagcgac gttgcaggga agcatgagac cgatgttctc ctcggcgaga
     5101 atcgcctggt aggcataggg aggattgcag gcgcccagga taagatatcc ggccatatct
     5161 acgcctaact tttccttcat ttttctctgg atatcgatct ccgccagcac cccgaaccct
     5221 tcttttccca gtgcctcccg cacctgagca atgaccgcct gacaaggaac agccaactct
     5281 ttggtgaatc cgtaattaat catacgcaac ctccattgta ctctctccac agtccggtaa
     5341 tgcttccgga gaaaacgctg ccccttggag gaccgttcag acacgccact tctgtgcttt
     5401 gctccggtat cattccgggg gcacttccat gagcggagga gtaaattgcg gtttatattc
     5461 gattttctga atatcgctcc tgagagcctg actaagaaaa cccgcaatgt gctcctgaag
     5521 ctgctcccag ccctgatccg atgactgctg cttcatgtcc ctttacctcc ttccttattt
     5581 ccggatacta tcctcaccgg aacaacttgg cggcaatttc tgccacgtgc gttccctggt
     5641 agcgcgccgc tgcgagctcg ttttcactcg gcatccgcat gccatcgcct ccggcaatcg
     5701 tcgaggcccc atagggcgat cctcccgtca cctcgtcgat tcgcatttgt ccggcgaagg
     5761 tataaggaag tccgacaatc accatgccat ggtgcagcag atttagatga aaggataaaa
     5821 tggtcgattc ctgcccgcca tgctgggtgg cgctggaggt aaatacgctc cccaccttgc
     5881 cgaccagggc gccccgggac cagagcccgc cggtgccatc gagaaactgg cgcatttgac
     5941 cgcacatgtt gccgaaacgg gtgggagtgc cgaagatgat ggcatcggaa gccgccaagt
     6001 cctccgtttg gcacacaggg acatgggcct gggccttttg cgcctcgaga gcgcccatct
     6061 tcgccaggac gtcttccgcg agggtttccg gaacgcggca gatcccgacc tcggcaccag
     6121 tcacgctgcc cgccccttct cctaccgcct tccccagccg gtggatatgg ccgtacatcg
     6181 agtaatagac aatcaatact ttcataacgc ctctcctttc cttttcgaga taaatgttct
     6241 tctagccgtt tctggcagga atcatcatat gcggccggtg tcgatgcctg ttaagattgg
     6301 gatgagcaat tagcctgcca acaggggcaa gagaaaggaa gtagcgttat tgttgtataa
     6361 taacaactgg ttatcttgaa tcggggaagc ggcggttaaa gtgggaaagg tgtttttgac
     6421 ctcaatatat ggtggggcct caatatattg agggacatgt cacggatcgt gacatgggta
     6481 aagagtttat atgaaaacct ccgcaaataa ccggatattt tcatgttttg ctgtacccga
     6541 ccggggcagg aaggttaatc agtttccccc cttcccggcc ggacgatccc gagcttgtgc
     6601 atcctggcgc gtagcgtgct ggggtggaga ccaaggattg acgcggcgcc ttcctttcct
     6661 tcgatgcgcc agccggtgtc ggcaaggatc ctgaggatgt gatttcgttc cgtctcgacc
     6721 agcgttttcg tggcagcgga aaggacggga ggcgagatct ccaagttgtc cgcaagctgc
     6781 agcaccgaac cgggggcgag gattacggcc ctttcgataa cgctttccag ttcccggaca
     6841 ttcccgggcc atgagtaatc tataaggacc tgcatcatgt ccctggggat cgaatcgatt
     6901 tgtttgccca tcttccggga aaggcgctcc acaaaggccc ggaccattat cggaatgtcg
     6961 tctttacgct gtcggagcgg gggaacggtg atgggaaaga cattgagccg gtaataaaga
     7021 tcctgccgga accgtccctt gcggacctct gcttccaggt cccggtttgt cgtcgcgata
     7081 atccggacat cgaccttgac ggtgcaggac gagcccaaac gttcgaattc gtggtgctgg
     7141 atcacccgca gcagtttggc ctgcaactcc agcggcattt ccccgatctc atccaggcag
     7201 agggtggagc cattggccac ctcgaagcga cctacctgcc gggtatcggc cccggtgaac
     7261 gcccccttct ctctgccgaa cagctcactt tcaatcagat tggccggcag ggcggcacaa
     7321 ttgacggtga tcatgctccg ctccttgcgg gggctcatgt cgtgaatagc aaaggcgaac
     7381 agttccttgc ccgtcccggt ttcacccagg atgaggcagg ttgtagttgt gggggcaacc
     7441 tgttccgccc ggtaaagggc gtatctgagg gcttcgcttt gcccgagtat cgcggcatgc
     7501 tgatggtgta gcttgatctc ctggcgaaaa tagatattct cggcctccag gcgatccttt
     7561 aaagccttca cttcggttaa tgctgtccga agctctgccg tccgctcctc aacctgctgt
     7621 tccagcatgt cgtgggcctt taccagttcc tcctccatct ccttgcgctc ggtgatgtca
     7681 tgcaccatcc cgaccgaccg gacgatccgc cccgaggcat cgcggatatg gttgcaccgc
     7741 tcctggacag tgcgtatttc ccccgtcgcc tttctgacaa tgcggtgttc gatttcatag
     7801 ccggcctggc cgccgcgcac cgaatttgaa taggcggcat ccaccgcgta gcggtcctca
     7861 ggatgcacgg ccgccaggaa agtctcgtag gtggcggtaa actcctgcgg ctgggcgccg
     7921 aaaatccgat atacctcgtc ggaccaagac agccgattgt tcacgacatc caattcccaa
     7981 ctacccagat gggcgatccg ttgtgcgtgg ttcagtctcc gttcattttc acggagcgcc
     8041 tcttcacccc gtttgtgctc ggtaatatcg atgcaggtgc ccacccattc cagcacactg
     8101 ccgtcatctt tgaagacggg cacaccccgg gccaggaagg ttctgtaaac cccatcgtat
     8161 ctgcgtaagc ggtattcgac ttcataccgg tctccggtcg caacggcatt tttccaagtc
     8221 cgctccgcct cttcacggtc atcggggtgc agagcccgcg tccatcccgc ttccttgacc
     8281 tcctctttgc tctgaccggt aaaccttcgc cacgccggga tgtcttccgt gacccgacca
     8341 ttgacatcgg tcgtccagcc aagctgcccg gtcatttcta tgtacgaccg gtggcgcttc
     8401 tcgctcctat ccagctccca ggtgcgttcc gcgaccagga tctccagatt gtcgcgatgc
     8461 ctcctgagct cttcttgcgc ctgcctgtgt tcggttatat ctacgcccac ttccatgatc
     8521 atccgggcgc cgttggcgtc cgtgaatgga aagtcaaaga tgtcgtaggt gcgcccgtcc
     8581 ggtcctagcc actcccagcg atggggctgt ccggtctgca tgaccttgaa gctctcgcaa
     8641 ttttcgcacg gcgcggtgcg gcaaaacagg tattcaaaac agtgcctgcc ctgggaatcg
     8701 ccgaagcggc ttctgaagaa acggttggca aacggtaccc ggtagtcttc agaaaggagc
     8761 gccacatata cggggagcgt ctccagcaca ccgtacagtc gctggtgcgc ggcctcggcc
     8821 attgcccggg ccgactccag ttccgccgtc ctttccagca tgcgaacttc gaggttatcc
     8881 tgctcatgct ggagactttc ctcagcctgc tgtcgggcgg cgatatgggt ctgcaacagt
     8941 tcctcggcct cctgcaggcg cctttgccca tcggcaagtt tcaaaagaag ttcatcccat
     9001 tcggtatgcg ttggctgctt tgcctttggg tttgttttgg attttcgtgt aataaaatta
     9061 tccctccttt atttctctta gcaagttatg tcatattatc ctatatatat gaatatttca
     9121 attaaatacg cttttattgc ttcggcagta tagtatagca atcttaacaa tccaaattac
     9181 ggcaaatgca aaccttctat tagtgtcccc tcggagatgg ttgaaaccca tcgcctttat
     9241 gttggaggtt cttcgaaatt ctggagtaat gagcactata taccgttata caattcgttc
     9301 gacacggaca cggaatacat cagcttgaag ccgattatga ttccgtttcc cctttatata
     9361 ctacgcataa caggttcaaa gctacgacgg gagcccgtta ttccaaaatc acactttcaa
     9421 gaagtacgca ttaaccggat gaacctaaaa aacgattccc atcatggttg ggatagtgac
     9481 cacgccgaag acgcccgtca cctataacat tcccgcaagg ctttcctttg ccgccggata
     9541 catgagaaca ggaaacagcg gttaacaggg accgagaaca aagatggtga agaggcccca
     9601 ggagaaataa acgagtccga aggcaatgag tgcccaggcc gcccctcatt gacctggaag
     9661 cgggtgatcg aagtgatggt aatccttcat ggttttgaaa tcggcccccc attgccagcc
     9721 tcggcggagg aattcccgga ccacggggtg ctcccgggta aggactccgg gctccccggg
     9781 ccggtaaacg gctccccggg gggatatgcg cccgtgatca tagacgacgg gattccggaa
     9841 ggggttgatg tccacggcga gtcccagggc gtggcgcgac aatcgcttcg tccccgccac
     9901 ggtccgatag ttgaaggcgg aggtgttgtt gtcccgcatg gaggccgtat cggaccagcg
     9961 ataggcgacg atgggcacgg ctttcccgat aggaaaccta gtaatatcaa taagttgaaa
    10021 gatttccacc acgtcctgcc gcacggcccg atgcaccacc aactgcccct gatggcgccg
    10081 cccgtcgaag gagtaataac ggacatcaac gaggcatagc tgcgcgatga cctccggcgg
    10141 ggccttggtc ccggcgatgg ccgccgcgaa ggtcatccgg ctgtctacga ttaccggacc
    10201 gtccgccggg gcattcccca gcggcgcggc ggcggggcca ccatcagggg acgacgcagc
    10261 atggccatcc ttgggcgggg cggatttatc ggcggcggca gtcatgtaag tcccccctcc
    10321 tgagatcaca acaaacaaca gcaccgtaat aagacatatt attctcctgc cgagcagaaa
    10381 cggcgtcttt gacggcaaag cgcctttttt gtcatccatg gggcgatccg gtaaaaagtc
    10441 tcaagacatc ctccccgtca tcaccaatca ctggcccgtc acccgagata tttatcaata
    10501 atttcgccaa cttcctccgc atgcagggac acctccccat aaatttccac ggccagggga
    10561 gcggtcatcc ggaggaaatc cccctcccgg acggtaatga tcacgccgcc catctccacg
    10621 gcccccggcg tcaccaggag ggcgtcgtcc ccttcccggt agtaggcgtc gggacggtgt
    10681 ttccgccggg gaaagaggaa caggcgccag cgtccctccc ggtgagtcgc cagcagattg
    10741 aggagcggtt cttcctcccc gctgtccgtc gctgtcccca gcgcagcaat aagctccgta
    10801 aacaaggcgg ccagcgcctc acggtccgga ccttccatga ccacagcgct ccggccgggc
    10861 aggttccaga cgccgaactt cacccccggc cggttcccgt tcataattct gctccccgct
    10921 tcccactctt tttccacggg aacggcgccc gccggacaaa tctgaaagtg atggtgatcc
    10981 ggcaccgagg ccccgcagcg gggcccgttg taaaagaccg tgaaatcatt ccccaggtcc
    11041 ttggccaggg tgagaagcgt accgacatgg tccgccagac gctggggaag gtgccggaca
    11101 tgggcgacgg tgaagtgggg cgaaaagatc ggccggggat tgacgaggat caggaaatcc
    11161 ccgcggtagc ggacggcctg ctgcgccgcc ggcaggcggt aatgacatag gaagcagggg
    11221 cgacgccgca ccgatgaggc gtcaacggcg gcggtggcgc tgaccgaccg gcccggattg
    11281 tactgtacca gcagggtatg gccggcggca ttcagttccc ggagccacgc gccgttcatc
    11341 tccatatggg cggcagcaaa ctcctgccag ttgtagagct gctgggcaaa cagctccccg
    11401 caccggcggg cgagggacgg ctccccgggc ataccggtaa attcgctcca gatcagttcc
    11461 ggggcgacat catcgtcgat aattgtcaag attgcacctc caggatatga cgattataac
    11521 aaaccttgaa caccctcccc ccttcaatgg ggggcgagcg atatgaggtc ttagttccct
    11581 atagctccct ccccttctaa ggggaggaat aagattgcct tttacggggg cgtcagattt
    11641 gacttgccat gtaatccgca tcaccggcga caaggcgctg cctcgccatg atctccatcg
    11701 tccgcagccg gtccttgtag gcgtcgt
//
LOCUS       DAOSMF010000059        11693 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_6346_length_11693_cov_15.223406, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000059 DAOSMF010000000
VERSION     DAOSMF010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 11693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11693
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6346_length_11693_cov_15.223406"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="PLT63_06950"
     CDS             <1..233
                     /locus_tag="PLT63_06950"
                     /inference="COORDINATES: protein motif:HMM:NF015737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HOD98496.1"
                     /translation="FLYDFIGDQQQATATFTGGGASFATKGFDPPRASGNLGAKLTLM
                     TKNNLSFALNYDYEWKAGYYSHNGYFHIRYAF"
     gene            411..1253
                     /locus_tag="PLT63_06955"
     CDS             411..1253
                     /locus_tag="PLT63_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98497.1"
                     /translation="MVQKFRGLSEALTGLILMLIFVWCCSGCVGPGGAVADRIGSRAG
                     FEKTFLWTREFTLTAYWRGRRPGAPLNLYLEGDGSAWISRSWRSDDPSPRDPVALALA
                     ARDPGANVAYLARPGQYAAPGSPPVDPAFWSDRRFAPAVVAALGEAADLLLAKTRAKG
                     VNLIGYSGGAALAVLMAVQRNDVLSLRTVAGNLDPEAVNRHHRVSPLAGSLNPMDAAA
                     QLRDLPQRHFAGAEDAVVPPFAAGNFIRRQGGDDRRVIIVPGVGHGNGWRERWWELLS
                     FPLF"
     gene            complement(1245..1367)
                     /locus_tag="PLT63_06960"
     CDS             complement(1245..1367)
                     /locus_tag="PLT63_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98498.1"
                     /translation="MAQHKKIERKREIDRRRKRRKERLRAKAREQRAAEAKKSK"
     gene            complement(1441..2382)
                     /locus_tag="PLT63_06965"
     CDS             complement(1441..2382)
                     /locus_tag="PLT63_06965"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOD98499.1"
                     /translation="MRVHFWGTRGSLPASLKASQVRAKVIGALRAAQGKVFGDEHALA
                     AFVDRELPFSIRGSYGGNSSCIEIRGGEDYVICDAGTGLRDFGNSLPGARGVFHIFLS
                     HLHWDHIQGFPFFTPAYIPGNRIKIYGFHPGLEAAFATQQSPPFFPIPLPAMRAEISF
                     HPLRVDEIYEFAGFQVRGARQHHPGDSYGYRFTKNGRSVVYSTDSEHKKEAECSNYAY
                     LDFIRDTDLLIFDAQYTLLDAIGDKEDWGHSSNVIGVEMAVEAQVKRLCLFHHEHTYD
                     DETFDELLADTRTYLKILSETSALEIYLAYDGLEIGI"
     gene            complement(2415..2912)
                     /locus_tag="PLT63_06970"
     CDS             complement(2415..2912)
                     /locus_tag="PLT63_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOD98500.1"
                     /translation="MPIYEFYCHKCNTVYSFFSKGVNTDKIPKCPKCKRINLKRQMSV
                     FARIKTGGEEGPEAAGMPPIDEAAMEKAMTMLAGEADKLNEDDPRQAADLMRKLTEAT
                     GMNLGPAMEEALRRMEKGEDPEQIDAEMGDLLEAEEPFLFDGPKGKKGAAKSKPQIDE
                     TLYDL"
     gene            3750..5378
                     /gene="dacB"
                     /locus_tag="PLT63_06975"
     CDS             3750..5378
                     /gene="dacB"
                     /locus_tag="PLT63_06975"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HOD98501.1"
                     /translation="MKNRSPRASNKYRYLCCGLALLLLGAAVPLAADTVVIPDEIQAI
                     VSAPRYCHAWWGLLAQDVHTGRIICAWHPDLLFHPASVTKLFSAAAALEQLGGDYRFH
                     TPLYINGNLDSRGVVRGDLILVASGDITMGGRTRPDGRIAYSGIEQSAVGDKSGVQNA
                     PAKTNPLAGIRRLARQATSAGIKAVTGEVIVDDRLFPTSSVYSVKNPGMVIFHRSPIM
                     INDNLFDIVISPTRRGMPAAVDWRPRLLPLLQVVSTVRTVAPAQTPRLEVTPSGFGKF
                     LISGEIPEGHPPIVRETAVPDAASYARALLIAALRKEGIKMKASPATPNPFRRLPQAA
                     AYQRFVKVAELVSPPLTEYLRMILKDSHNPGAEIIPHLLALQAGGNSFAEGMQRQKLL
                     LSRFSADPGSISLADGSGLSQANLVTPRAVVALLAHMTRQRHGLFFREALPLLGVDGT
                     LMRSGRDSPARGRISGKTGTLITVDLLHDSAIMDGKSLAGYMMTAAGREVVFAFFVNF
                     VHSTDVRGREETRKFTGEIGADLLRIAEAIHLAN"
     gene            complement(5466..6749)
                     /locus_tag="PLT63_06980"
     CDS             complement(5466..6749)
                     /locus_tag="PLT63_06980"
                     /inference="COORDINATES: protein motif:HMM:NF036774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgO family outer membrane protein"
                     /protein_id="HOD98502.1"
                     /translation="MKRLLLLLSICTLLTLGGCAATKKAERTDPASLLHVNGTVQEIS
                     GSEITLLLKAPEWEKASASPIQDIARRVIAKSILIEGIATDMDGKPAIVREVRGNSVR
                     VSLTEPANYPPGTPVKLKIPKKTIAIVDFEVIKGKEKEIGRVTMEELTSALIDSGQFV
                     VVERSKLKAVMAELQLSRSGLAGETSEQAIGKLLMAELLLTGTLSEQRGEWNINLRLV
                     NVRSGQAVAAINMRSGLFKPSELRDSGQLPGDFEEAFLDPSWLAIRVGKTTFYDASVD
                     RTSGYEGSKRSMRIDFHLQKGVKPLMARVENRKKRDLTLYDGIEFYARATEKMRIMAM
                     ILTSQPDDPNKIDGWMGMAIVDKDWEKIRVPFDGMVIARGWIKQGAAGYGSQSGDQVL
                     RLNRVEDIQIGVHQGHNRDNLQGTIWLDGIRFYRD"
     gene            complement(6817..8220)
                     /locus_tag="PLT63_06985"
     CDS             complement(6817..8220)
                     /locus_tag="PLT63_06985"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HOD98503.1"
                     /translation="MTVIDRLQRLTKEAAIPPRKAFLTPGEGPPAGEAHRSSGESEAS
                     CLKIGALRERIDAILARRPGGGTDVSPPCRQDPGHPLEELVSGTEITTAGGRFFCAAS
                     TWGMGHCHGGQRLGDLPSLDMDGAAILANDALLAGLDPTRGVFLDTETTGLAGGTGTV
                     AFLIGLGWFEGERFVVKQLFLRDFAEERAVLLFLKELMADRQFLVSFNGKAFDANLLA
                     ARFVMNRLADPLAGLPHLDLLHPARRLLRRRLDNVRLGTLEGALLGLEREGDIPGWEI
                     PQRYFQWLRHRDGRLVADIFAHNRLDVLSMVFLAARLTELIAGPVSGAAALTPPDSDL
                     LAAARLCIQRGQTARAESILTDLRRRSGPITARQAAALLSLIHKRAGFWTRAVGIWQE
                     MLVMDRDSGGDALFPLLELAKWNEHRAHDYRTALNLACRALAMLPPQGTAAEAEDLRR
                     RIARLKRRLAGRDHPAT"
     gene            complement(8275..10050)
                     /locus_tag="PLT63_06990"
     CDS             complement(8275..10050)
                     /locus_tag="PLT63_06990"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD98504.1"
                     /translation="MGATEALREALLDLEEARKLEAQQRRMAEALLAGLHVLVASGSA
                     TDIFPKLFDVLREPLDFRAAFVLAAGSDGLLVPIAASDVRFKNTLWNPGTAFKRAMKG
                     KPIAVFDTEAVEEWRRQGDELRKQNRSALVFSVGTETRPALFICTHPARGHFSLLHVN
                     LARRFSVLAVQALMKMEADQRVANLEERLKNEARLAELNRKLAESEKKLARAGKLEAL
                     GLLAGSVAHDLNNILSGIVSYPDLLLMSPDLPQKQRRIIETIRASGLRAAAVVQDLLT
                     VSRGVAVIQDTVRLNDLIEQFLAGPEYLEIMKDTEGISVQVNLDERPRNIRASRIHVE
                     KALANLFVNALDAVRGGEDGIIGIKTETVHVDRPRNGYEDIALGEYAILTVSDNGPGI
                     SEEDRERIFEPFYTKKKMGKSGTGLGLTVVWNIMQDHNGYIDLRTGGKGTQFSLYFPV
                     VRESIADREPLAALSDYRGRGERILVVDDQEEQRLIACNMLSSLGYEAVAVAGGEEAV
                     AYLENEAADLVLLDMVMSPGIDGRETYERIIRIRPDQKAVIASGYSLNDDVLTVQRLG
                     AGAFLKKPYSLEELGMAVKRELFRG"
     gene            complement(10054..11259)
                     /locus_tag="PLT63_06995"
     CDS             complement(10054..11259)
                     /locus_tag="PLT63_06995"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HOD98505.1"
                     /translation="MKIAVSWSINPDSAAAAEEAFRILMEKLADKPRLLLVHCSCLYD
                     HAALLSRLTDLAPDVPIQGGTSCLGVMTGEGFHSRDGFGLGLLGIADSEGDYGAGMAD
                     IGADPQKAARNALQEALARAGHPGEAPAAILVSSAPGKEELLIQAIEEHVGSGVPIIG
                     GTAADNDMSGQWRQFADGRVSEGAVSIAVLFPSGDIGFSFHSGYEPTEHKGRATRTSG
                     RILHEIDGRPAALVYNDWTGGLLTDILPAGGSLVPTATFTPLGNPVGHVRGVPYYRLS
                     YPVEALPDGSLLLFTDVRTGDEIFLMRGNPGNLVSRTGRAAAAALDAAPFDAADARGA
                     LVLFCTGCLLAIKDRIEEPRASLKAILQDTPFLCAFTLGEQGCFIGGENRHGNLMVAV
                     LVFGPARRE"
     gene            complement(11440..>11693)
                     /locus_tag="PLT63_07000"
     CDS             complement(11440..>11693)
                     /locus_tag="PLT63_07000"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOD98506.1"
                     /translation="GFNIEADFRKCEFSILVHDDFQGQGLAHKMIDILIGIADEKGLE
                     EFYGYIEPQNRKMVRLCEKLGMSAEKSPDDLTRVSLRLR"
BASE COUNT         2180 a         3598 c         3489 g         2426 t
ORIGIN      
        1 agtttctcta tgacttcatc ggcgaccagc agcaggccac cgccaccttt accgggggtg
       61 gcgcctcctt tgcgaccaaa ggctttgacc caccccgggc gagcggcaat ctgggggcca
      121 agctcaccct gatgacgaaa aacaatctct cctttgccct gaactacgac tatgagtgga
      181 aggcgggata ttacagtcat aacggctatt tccatatcag gtacgccttc tgagtcgcca
      241 ggccgtaggc ctttgcccac gtcctcccgc gccggctttt ggcaactcgc aaaccgctcg
      301 ctgcatcaag tcggaaccgg tccggccggg ccgccgggga tggcgacggt ttacgaagtc
      361 gggcggccag tgggatgact ggtgagggag cgtatgcaag gttcgcagct atggttcaaa
      421 aatttcgtgg gctttccgag gccttaaccg gactcatcct gatgctgatc tttgtctggt
      481 gctgttccgg gtgtgtcggt ccgggtggag cggtggccga ccggatcgga tcgcgggcag
      541 ggtttgaaaa aacctttcta tggacccggg aatttacgct taccgcttac tggcggggcc
      601 gccggccggg agctcccttg aatctctacc tcgaagggga tgggtccgcc tggatttccc
      661 gttcctggcg ctccgacgat cccagccccc gagatccggt ggcactggca ctggcggccc
      721 gggatcccgg ggcgaacgtg gcctatctcg cccggcccgg ccagtatgcc gcgccgggat
      781 cgcccccggt cgatcccgcc ttctggtccg accgccgctt tgcccccgcg gtggtggcag
      841 cgctcggcga ggccgcggac ctccttttgg cgaagactcg ggcgaagggg gtcaatctca
      901 tcggctattc gggcggggcc gccctggccg tgctgatggc cgtccagcga aacgatgtcc
      961 tgagcctccg gaccgtggcg gggaacctgg acccggaggc ggtgaaccgc catcatcgcg
     1021 tcagccccct ggccgggtcc ctcaatccta tggatgcggc ggcacaactc cgtgacctgc
     1081 cccagcgtca tttcgcaggc gccgaggatg ccgtggtgcc ccccttcgcg gccgggaatt
     1141 ttatccgccg tcagggcggg gatgaccggc gcgtcatcat cgttcccgga gtgggccacg
     1201 gcaacgggtg gcgggaacga tggtgggaac tgctgagctt tcccctattt tgatttcttc
     1261 gcttctgccg cccgctgttc tcttgccttg gcccggagac gttccttccg ccgtttgcgc
     1321 cggcgatcga tttctctttt ccgttcgatc ttcttgtgtt gtgccattcg tttctccctg
     1381 taaatttatc agtattccaa actatttacg aataaaacac cgcgggcgga tcaatgccgt
     1441 tcagattccg atctccaagc cgtcgtaggc caggtatatc tccaaggcgc ttgtttcgga
     1501 tagaatcttc agataggtcc gcgtgtccgc cagcagctca tcaaaggtct cgtcatcgta
     1561 ggtatgttcg tgatggaaca gacaaaggcg tttgacctga gcctccacgg ccatttccac
     1621 gccgatgacg ttgctggagt gcccccagtc ctccttgtcc ccgatggcgt cgagcagggt
     1681 gtactgggcg tcgaagatga gcaggtccgt atcccggatg aaatcgaggt aggcgtaatt
     1741 ggaacattcc gcctcctttt tgtgttccga atccgtggaa tatacgacgc tccggccgtt
     1801 tttcgtgaaa cggtacccat aggagtctcc gggatgatgc tgtcgcgccc cccgtacttg
     1861 gaaacctgca aactcataaa tctcgtccac ccgcaggggg tggaaggaga tctccgcccg
     1921 catggccggc aggggaatgg gaaaaaaagg cgggctttgc tgggtcgcga aggccgcttc
     1981 gaggccggga tgaaaaccgt agattttgat ccggttgcca ggaatgtagg cgggagtgaa
     2041 aaacggaaag ccctggatgt ggtcccagtg aagatgagat aggaagatgt ggaagactcc
     2101 tcgggcgccc ggcaggctgt tgccgaagtc ccgcaggcct gtgccggcat cgcagatgac
     2161 gtaatcctcg ccgccccgga tctcgatgca ggaactgttg ccgccgtagc ttccccggat
     2221 ggaaaaaggc aactcccgat ccacgaaggc ggcaagcgcg tgttcatcgc caaagacttt
     2281 tccttgggcc gcccggagtg ctccgataac cttggcccgg acctgggacg ccttgaggga
     2341 ggcgggcagg gagccccgag ttccccaaaa atggacgcgc atgtagtgcc ccctccttta
     2401 ccgatatgta tcgtctatag atcgtaaagg gtttcgtcga tttgcggttt tgacttcgcg
     2461 gcccctttct ttcctttcgg accgtcaaac aggaacggtt cctccgcttc caggagatct
     2521 cccatttccg cgtcgatctg ttccgggtcc tctccctttt ccatgcggcg cagggcttcc
     2581 tccatggccg gaccgagatt catccctgtg gcctcggtga gtttccgcat gaggtcggcc
     2641 gcctgtcggg ggtcgtcttc gttgagttta tcggcttcac cggccagcat ggtcatggcc
     2701 ttttccatgg ccgcctcgtc gatgggaggc atgcccgctg cttcgggacc ttcttcgccg
     2761 ccggtcttga tcctggcaaa tacggacatc tgacgtttta ggtttatcct tttgcatttc
     2821 gggcacttcg ggattttatc cgtatttacg cctttggaaa aaaagctata gacggtgttg
     2881 cacttatgac aataaaactc gtaaataggc atccgcaatg ctcctgaaat ttttttcagc
     2941 aatataatca tcctgttagg aaaagcaagg gaaattttat cttggtttcc ctgacacgat
     3001 acgccgcccc gcccctgatc ttctttctct aaaaatcgag gttgatccga acgcgtccaa
     3061 ttcatcattg atgccgccgt gaacggtacg tacggggaag taggcgcact caaggcccag
     3121 ccctctccag ggacggaatt ccgcgagcag gaagggcacc cccacggcga cccggtagcg
     3181 ccggtccttt tgaaagacat agccaatgcc ggggatgggc atgtgggccc agaattcccg
     3241 gttgttgttg taattgagaa agagcagccc cccggagctg ggcggccgcc ggcgccgcca
     3301 ggatgagcag ggccggcagg caaagcagca ttaagaacat cgagcgggaa aaaatgttat
     3361 catgattatc ggacctgata caatcattgt ctgatgaaaa gcaaggcctt tttgccgttc
     3421 taaaccagcc cggcaattga cattgacagg acgaggtctt ttttgcatag aaaacggaag
     3481 atgcagtcat cgcctcttgc cagcgaagag acgatcaaaa tatgatgaca tcgtaaaagg
     3541 tcaaaaaatg tcccatttgg gacggcatcg taaaaagatc cgcaggcgag gcgcgcgcat
     3601 cctgaggaat gaggcgtaca tggacgtacg tcgcagtgac gagggatgaa gcgctttcga
     3661 agcatccgga aagcgaagct tcatgtccat actttttacg atgccgtcaa atttgaaaag
     3721 attcccgaaa gagatcaggc ccgaaggcca tgaagaatag atcgccacga gcaagtaata
     3781 aataccgcta cctgtgttgc ggtctggccc tcctgctgct gggcgccgcg gttcccctgg
     3841 cggccgatac ggtcgtaatt ccggacgaga tccaggccat tgtctccgct ccccgctact
     3901 gccatgcctg gtgggggctg ctggctcagg acgtgcatac cggccggatc atctgcgcat
     3961 ggcacccgga cctccttttc caccccgctt ccgtgacgaa actcttttct gcggccgccg
     4021 ccctggagca actcggcggt gattatcgtt tccatacccc cctttatatt aacgggaact
     4081 tggatagcag gggggttgtc cggggggatc tgatcctcgt cgcctccggt gatattacca
     4141 tgggcggccg cacccgcccg gacgggcgca tcgcctacag cggtattgag caatccgccg
     4201 tcggcgacaa gtccggagtg cagaacgccc cggcgaaaac caaccccctg gcggggatcc
     4261 gccggctggc ccgtcaggcg acgagcgccg ggatcaaagc cgtcaccggc gaggtcatcg
     4321 tcgatgaccg cctgtttccc accagtagcg tttacagtgt caaaaacccc gggatggtga
     4381 ttttccaccg cagccccatc atgatcaacg acaatctctt cgatatcgtg atctcaccca
     4441 cccgccgggg gatgccggcg gcggtggact ggcgtccccg gctcctgccg ctgctccagg
     4501 tcgtctcgac ggtccgcacc gtggcgccgg cccaaacgcc ccgtctggaa gtgacgccgt
     4561 cgggctttgg aaaattcttg atttccggtg agatcccgga aggacatccc ccgattgttc
     4621 gggagacggc cgtccccgac gccgcctcct atgcccgggc gctcctcatt gccgccttgc
     4681 ggaaggaggg catcaagatg aaggcctccc cggcgacgcc caatcctttc cggcggctac
     4741 cccaagcagc ggcttatcag cggttcgtca aggtcgccga gcttgtctcg ccgcctttga
     4801 ccgaatatct ccggatgatt ctcaaagaca gccacaatcc cggcgccgag atcatccccc
     4861 atctactggc cctgcaagct gggggaaatt cattcgcgga aggcatgcaa aggcaaaagc
     4921 tgctgctgtc ccgatttagc gccgatcccg ggagcatttc cctggccgac ggctccggcc
     4981 tgtcccaggc caatctggtt accccccggg cggttgtcgc cctgctggcc catatgacca
     5041 ggcagcgtca tggccttttt ttccgggagg cgctgccgtt gctcggagtg gacggcacgc
     5101 tcatgcgcag cggccgggac agtcccgccc ggggcagaat cagcggtaag acgggcacac
     5161 tgatcaccgt ggatctgctc cacgacagtg ccatcatgga cggcaaatcc ctggcgggat
     5221 acatgatgac ggcggccggc cgggaagtgg tctttgcctt cttcgtcaat tttgtccaca
     5281 gcaccgatgt acggggaagg gaagagaccc ggaagtttac cggggagatc ggcgcggatc
     5341 tcctccggat cgccgaggcc atccacctcg ccaactgaac taaggttagc ggttagcggc
     5401 gttttaggag cgtcgccggc ccgattccgg tcaatatccg gacgggcgca ttatccacaa
     5461 ggtgatcagt cgcggtaaaa acgaatcccg tccagccaga tcgttccctg cagattgtcg
     5521 cggttatgcc cctgatgaac tccgatctgg atgtcttcaa cccggttgag gcgcaggacc
     5581 tggtctccgg actgggaacc gtatccggcc gctccctgtt tgatccagcc gcgggcgatc
     5641 accatgccgt caaaggggac gcggatcttt tcccaatcct tgtccacgat ggccataccc
     5701 atccagccgt cgatcttgtt gggatcgtcg ggctgagagg tgaggatcat ggccatgatg
     5761 cgcatctttt ccgtggcccg ggcatagaac tcgatgccgt cgtagagggt caaatcccgc
     5821 tttttcctgt tttccaccct tgccatcagg ggtttaaccc ccttttgcag atgaaagtcg
     5881 atccgcatgg atcttttcga tccttcgtag ccggacgtcc ggtccacgct cgcatcgtaa
     5941 aaggttgtct tgccgacgcg gatggccagc caggacggat cgagaaaggc ctcctcgaag
     6001 tcccccggca actggccgct gtccctcagt tccgaaggct tgaatagacc ggagcgcatg
     6061 tttattgccg ccaccgcctg gccggaccgc acgttgacca ggcgcagatt gatattccat
     6121 tccccccgct gttcggaaag ggtgccggtg aggagcagtt ccgccatgag caacttgccg
     6181 atggcctgct cggatgtctc cccggccaga ccgctcctgc tcaactgcaa ttccgccatg
     6241 acggctttca gctttgacct ttccacaacc acgaactgac ccgagtcgat caaagccgag
     6301 gtaagctctt ccatggtcac ccggccgatt tccttttcct ttcccttgat gacttcgaaa
     6361 tccacgatgg cgatggtctt cttggggatc ttcagcttca ccggggtgcc cgggggataa
     6421 ttcgctggtt cggttaggga cacccggacg ctgttccccc gcacttcccg gacgatggcc
     6481 ggttttccat ccatatccgt ggcaatgccc tcgatcagga tgcttttcgc gatcacccgc
     6541 cgggcgatgt cctggatggg cgatgcggac gccttttccc attccggcgc cttcaagagc
     6601 agggtgatct cgctgccgga tatttcctgc acggtcccat tgacgtgcag caggcttgcc
     6661 gggtccgttc gctccgcctt cttcgtggcg gcgcagcccc ccagggttag cagggtgcat
     6721 atagacagta ataataggag ccttttcatg aaaatcctcc gtatttcttt ctcggaggag
     6781 ggcgacagat tcgctctctc cgatcattca tcaccttcac gtcgccggat ggtcccgccc
     6841 cgccaggcgg cgcttcaggc gggcaatgcg gcgccggaga tcttccgcct ccgctgccgt
     6901 cccctggggg gggagcatgg ccagcgcccg acaagcgaga tttagggccg tccggtagtc
     6961 atgagcccgg tgctcgttcc acttggccag ttccagaagg ggaaagaggg cgtcgccgcc
     7021 ggagtcccga tccatgacaa gcatctcttg ccaaatgccg acggcccggg tccagaaccc
     7081 cgcccgttta tggatcaatg agagcagagc agcggcctgc cgggccgtga tcggaccgga
     7141 gcgccgtcgg agatcagtaa ggatgctttc cgcccgggcc gtttggcccc gctggataca
     7201 aagccgtgcc gccgccagca ggtccgaatc ggggggcgtc agggccgcgg cgccggagac
     7261 cggtccggcg atcagctccg ttagacgggc ggcaaggaac accatggaga gtacgtcgag
     7321 gcggttgtgg gcgaagatgt ccgccaccag ccggccgtct cgatgacgga gccattggaa
     7381 gtaacgctgg gggatctccc agcccgggat gtctccctcc ctttccagtc ccagtaaggc
     7441 cccttccagg gttccgaggc ggacgttgtc gaggcggcgc ctcaggagcc gccgggccgg
     7501 atgcagcaga tcgagatggg gaaggccggc caggggatcg gcgagacgat tcatgacgaa
     7561 ccgggcggcc agcagattgg cgtcgaaggc cttgccgttg aaggagacga ggaactgccg
     7621 gtccgccatc agctccttca gaaagaggag gaccgcccgt tcctccgcga agtcccgcag
     7681 gaagagctgt ttgacgacga acctctcccc ctcgaaccac cccaggccga tgagaaaggc
     7741 cacggtgccc gtgccccccg ccagtcccgt tgtttccgtg tccagaaaga cgccccgggt
     7801 gggatcgaga ccggccagca aggcgtcgtt ggccaggatg gcggcgccgt ccatgtccag
     7861 agagggcaga tcgccgaggc gctgcccgcc gtgacaatgg cccatacccc aggtgctggc
     7921 ggcgcagaag aagcgcccgc ccgccgtggt tatctccgtt ccggagacaa gctcctccag
     7981 gggatgcccc gggtcttggc gacagggggg ggatacgtcc gtgccgccgc cgggccgccg
     8041 ggccaggatg gcgtcgatac gctcccgcag ggcgccgatt tttaaacatg aagcttcgct
     8101 ttcccctgat gaccggtgcg cctcccccgc cggcggccct tcaccggggg tcaagaaggc
     8161 tttgcggggc ggaattgccg cctctttcgt cagccgctgc aatctgtcta ttaccgtcat
     8221 ttgccggaga cctttccatt gccggtccgg gcggatggac ccatataaac cgcttcaacc
     8281 ccgaaagagt tctcttttga ccgccattcc cagttcctcc aggctgtagg gcttcttgag
     8341 aaaggcgccg gcgcccagcc gctggacggt cagaacatcg tcgttaaggc tgtaaccgct
     8401 ggcgatgacg gccttttggt ccggacggat ccggattatg cgctcgtatg tttcgcggcc
     8461 gtcgatgccg ggactcatga ccatgtcgag gagcaccaga tccgccgcct cgttttccag
     8521 gtaggcgacc gcctcttccc ctccggccac ggccacggct tcgtagccca gggaggagag
     8581 catgttgcat gcgatgaggc gctgctcttc ctggtcatcc acgaccagta tcctttcccc
     8641 ccggccccgg tagtcgctta gggccgcaag tggctcccgg tcggcaatgg attccctgac
     8701 taccgggaaa tatagggaga attgtgttcc tttcccaccg gttcgcagat cgatgtagcc
     8761 gttgtggtcc tgcatgatat tccataccac ggtcaatccc agccccgtgc cgctcttccc
     8821 catctttttt ttcgtataga agggttcgaa aatccgctcc cggtcctcct cggaaatgcc
     8881 cggaccgttg tccgataccg tcagaatcgc atattcgccc aaggcgatat cctcgtatcc
     8941 gttccgtggc cgatccacat ggactgtttc ggtctttatg ccgataatac catcctctcc
     9001 gccccggacg gcgtcgaggg cattgacgaa gagattcgcc agagccttct ccacgtgaat
     9061 ccgggaggcc cggatgttac gcggccgttc gtcgaggttg acctggacgg aaatgccctc
     9121 cgtgtccttc atgatctcga gatattcggg gccggcaagg aactgctcga tgaggtcatt
     9181 caaccgcacc gtatcctgga taaccgcgac gccccgggag acggtcagca gatcctggac
     9241 cacggccgcg gcccggagcc cggaggcgcg gatggtttca atgatccgtc gctgcttttg
     9301 cgggagatcc ggcgacatga ggagaaggtc gggatagctg acgattccgg agaggatgtt
     9361 gttgaggtcg tgggcgacgc tgcccgcaag aagccccagg gcctcgagtt ttccggccct
     9421 ggccagcttt ttctcacttt ccgccagctt gcggttaagc tccgccagcc gggcctcgtt
     9481 tttcaaccgc tcttccagat tggctacccg ctgatcagct tccattttca tcagggcctg
     9541 tacggccagc acggaaaaac ggcgggccag gttcacgtgg aggagggaaa agtgaccccg
     9601 cgccggatgg gtgcagataa aaagcgccgg cctggtttcc gttcccacgg aaaagacaag
     9661 ggccgaacga ttctgtttcc gcagttcatc tccctgccgg cgccattctt ccaccgcctc
     9721 cgtatcgaag acggcgatgg gtttcccctt catggcccgt ttaaaggcgg tgcccggatt
     9781 ccagagggta tttttgaaac ggacgtcgga ggcggcgatc ggcaccagga gaccgtcact
     9841 gcccgctgcc aggacgaatg cggcacggaa atccagtggc tcacgcaaga catcgaaaag
     9901 cttgggaaaa atatcggttg ccgatccgga agcgaccagg acgtgcagac ccgccaggag
     9961 cgcctccgcc atccggcgtt gttgagcctc cagctttcga gcttcttcga ggtcgaggag
    10021 ggcttcccgc aatgcctccg tggcgcccat ggcttattcc cttcgcgccg gtccgaagac
    10081 aaggacggcc accataaggt tgccgtgacg gttttcaccg ccgatgaagc agccctgttc
    10141 acccagggta aaggcgcaca gaaacggcgt gtcctgcaaa atcgccttca gacttgcccg
    10201 tggttcctct attcgatcct tgatggccaa aaggcaaccg gtgcagaaca ggaccaatgc
    10261 tccccgggcg tcggcggcgt cgaagggggc ggcatccagg gccgccgccg ccgcccgccc
    10321 cgtcctactc acgagatttc cgggattgcc gcgcatcagg aatatttcgt cacccgtccg
    10381 gacgtcggta aacagaagga gggagccgtc cggcagggcc tccacgggat aggacaatcg
    10441 ataatacgga accccgcgga cgtgccccac ggggtttccc aggggtgtga aagtggcggt
    10501 gggaaccagg ctcccccccg cggggagaat atccgtaagg agaccgcccg tccaatcatt
    10561 atataccagg gcggccggcc tcccgtcgat ttcgtggagg atccggcccg aggtccgggt
    10621 cgcccgcccc ttatgctccg tgggttcata accgctgtgg aaggaaaatc cgatatcgcc
    10681 ggaggggaaa agcacggcaa tggaaaccgc gccttcggac accctgccgt cggcgaactg
    10741 cctccattgt ccgctcatgt cgttgtccgc cgccgtgccg ccgatgatcg ggacgccgct
    10801 gcccacatgt tcctcgatcg cctggatgag gagttcctcc tttccggggg cgctgctgac
    10861 gagaatggcc gcgggagctt ctcccggatg gccggcgcgg gccagggcct cttgcagggc
    10921 gttgcgggcc gccttttgcg ggtcagcgcc gatatcggcc atgccggcgc cgtaatcgcc
    10981 ctcggaatcc gctatcccca aaagacctag gccaaagccg tcccggctgt gaaaaccttc
    11041 tcccgtcatg acgcccagac aggacgtacc accctggatg gggacatccg gcgccagatc
    11101 ggtgaggcgg ctaagcaggg cggcgtggtc gtaaagacag gaacagtgca ccagtaaaag
    11161 acggggcttg tccgccagct tttccatgag gatgcgaaat gcctcctcgg cggcggcggc
    11221 ggaatcggga ttgattgacc aggaaacggc aattttcatg atgaacctcc tgctgtgtgg
    11281 cgtcgcttcg tattcggtat agcgaccatg cccgcggtcg ggatcgccgc gccggagaac
    11341 ttacctgtga ttgaagcctg agttgaattt aaggcaattg ccggggagtg tcaagttttt
    11401 aagcattccg gggcggaatc ccgatcgtcc cgtctcgtct cagcgcagac gcagactgac
    11461 cctggtgaga tcgtcgggcg atttttctgc gctcatgccg agtttttcgc acagccggac
    11521 cattttccgg ttttgtggtt cgatatagcc gtaaaattcc tccaaacctt tttcgtcggc
    11581 gatgccgatg aggatgtcga tcatcttgtg cgccagtccc tgaccctgaa agtcgtcatg
    11641 gaccaggatg gagaattcgc atttccggaa atcggcctcg atgttgaacc ccc
//
LOCUS       DAOSMF010000060        11683 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_6353_length_11683_cov_25.348383, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000060 DAOSMF010000000
VERSION     DAOSMF010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 11683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11683
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6353_length_11683_cov_25.348383"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="PLT63_07005"
     CDS             complement(<1..202)
                     /locus_tag="PLT63_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98507.1"
                     /translation="MLKETADMSTPESGGVFDATPADHDDANNKASQVPIVASGAFIK
                     VAIATLGCKVNQYESAAMAECLT"
     gene            356..697
                     /locus_tag="PLT63_07010"
     CDS             356..697
                     /locus_tag="PLT63_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98508.1"
                     /translation="MEAIWSSIHTHQFAVVAIVIAALIVIYFLFKNLIKLALLFLLIM
                     ILTGGYFYLTAPKKSPEDLNRAIEQTQEQTAEIIDKGKVAYEKGKEVVERGKELTDRF
                     GKVIGGNPRRD"
     gene            complement(771..1265)
                     /locus_tag="PLT63_07015"
     CDS             complement(771..1265)
                     /locus_tag="PLT63_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOD98509.1"
                     /translation="MKALTKKDRSPNIAPTNSDGSEIMAEEQIVSRKIVIHYTADNVD
                     QPIICQLVKTHDLVFNILKARIFPKREGIIVLELSGNQRNFDNGIRFLKDKGLKVEPL
                     SKSVNQNSDKCVHCGACTAFCPTDALSFEPGTRKVVFDPEKCNGCELCVSACPVRAME
                     VNIF"
     gene            complement(1284..1751)
                     /locus_tag="PLT63_07020"
     CDS             complement(1284..1751)
                     /locus_tag="PLT63_07020"
                     /inference="COORDINATES: protein motif:HMM:NF019494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HOD98510.1"
                     /translation="MAEKLLYKKYGNRRLYDTAKSSYVNLEDITRAIRADHQVQVLDA
                     KTGEDVTAFILTQVILEESRKKNFLLPVPLLHLLIRFGENLLNDFFQQYLEQILNNYL
                     SYRSVTDNQFKKWLDLGIDYSAINPINPFKPFFDIFPPAAGAGEEDKKGKGNR"
     gene            2019..2318
                     /locus_tag="PLT63_07025"
     CDS             2019..2318
                     /locus_tag="PLT63_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98511.1"
                     /translation="MEQGKIAKQMIDFHKATFDNTFNAMSILQEQTERMVNMFLEQSP
                     WVPEEGKNAIQDWVKAYKKGRMDFKTSADDSFKKVEDFFAGFNKQAKETKVKAGK"
     gene            2347..2613
                     /locus_tag="PLT63_07030"
     CDS             2347..2613
                     /locus_tag="PLT63_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98512.1"
                     /translation="MDPKQISKQMFDFNKMAFERSFAAMVVMQDQAEKMFNMWLDQNK
                     WFPEEGKKAMSEWVKTYKKGREDFKSKVEEGYDKIEQYLAPAEK"
     gene            2653..>4096
                     /locus_tag="PLT63_07035"
     CDS             2653..>4096
                     /locus_tag="PLT63_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98513.1"
                     /translation="MISKYQQQVLKRAASLYTDRLTETPGKMLGVATDILKAGQLYGL
                     GVMAYFADFMVPFWTAWQSFNRLEKEKLRRTFFLQSLADYYELMQFNLQIAEQGMQGT
                     VNGFRDFYRKETAQALMSLLNSLYGEEEEDVGKYAARLVQLTDGVINRYPEAIKNIGG
                     EFGFHFDDDGYIKTAETDRFFLYQVLSRDKSITPRKDGKPVVIIHPYVLGPNILAFLP
                     DEKKSYVHAFADQGIPTYIRILKDIQTTEAVQIMTGEDDALDTRRFCELLMVTHGRPV
                     TLNGFCQGGFMALLDILSGELDGLVDALITCVAPMDGTRSRSLVEYMQHLPGRFRDLN
                     YAIKKLPNGNGIVDGKVMSWVYKLKSMEKEAPFVTFYRDLSNVGGEGISKTAAAMNHW
                     LIYDRIDLPEGITKLSFDSYTIPVTEDGTLPVKLFGRTLNFKGIQEKGIPWLLCYAER
                     DDLVDKDAALAPVDFIDVEVTPFPKGHGAIA"
     assembly_gap    4097..4196
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4363..4875
                     /locus_tag="PLT63_07040"
     CDS             4363..4875
                     /locus_tag="PLT63_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98514.1"
                     /translation="MDRNFYEFWGQMFLNLAKGQKQIEDFRKWFGQGLMGYESMNAMF
                     RKAYGLDRIEEESPDYAEIWKKSVETFQKSFREGLSLMEVVPKGEYEKLAQKCAALEK
                     TVAEQEETIRTLKRLLTEEASGAPAAALEDLQGLLRDQQTQFQELVKNMTLLFEKEPV
                     VTRGTKKKGH"
     gene            complement(5062..5481)
                     /locus_tag="PLT63_07045"
     CDS             complement(5062..5481)
                     /locus_tag="PLT63_07045"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HOD98515.1"
                     /translation="MKGRTIDTIKVGDAAEFTKTVTETDVYLYAGITGDLNPAHINET
                     YAAGTFFKGRIVHGILTAGFISAILGMQLPGPGTIYMSQSLKFLAPVRMGDTITARVE
                     VVEVITEKNRLRLQTTCRNQEGTVVLDGEALVSPPKA"
     gene            complement(5572..6621)
                     /locus_tag="PLT63_07050"
     CDS             complement(5572..6621)
                     /locus_tag="PLT63_07050"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOD98516.1"
                     /translation="MPEKQTSYLKTFIKVSDAFGTTTDMDALLQLIVDSAIGTMKAKA
                     ASLFLADEGREIFKAVAQRGLSADYLHAGYGHAEKIIPILKQDGFIYYKDATTDIRLE
                     NREGKKAEGIGSIIVVPAMVKGELIAILTLYTSKIREFTRDEIEFLQLLAGQGGWAIQ
                     IKKLVDQLRDNTKLFLNLAADIAASLDIKTILQTLTEEVAKALRVKAASIRLLNDERT
                     VLQLVASYGLSDKYLKKGPISAEKSIAEALNGKPVAITDAYTDKGVQYKKEKKEEGIV
                     SILCVPIKAKNEVIGVLRLYCDKKREFTEDEIGLVTALAYQGGLAIHNASMYLMLQND
                     LRDMKEATWTHKCWF"
     gene            complement(6637..7935)
                     /gene="phaC"
                     /locus_tag="PLT63_07055"
     CDS             complement(6637..7935)
                     /gene="phaC"
                     /locus_tag="PLT63_07055"
                     /inference="COORDINATES: protein motif:HMM:TIGR01836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III poly(R)-hydroxyalkanoic acid synthase
                     subunit PhaC"
                     /protein_id="HOD98517.1"
                     /translation="MEQPKISLDLILANIASEAEKAQETVTKASEVLLGPLESNLAMT
                     PYDVVYEEDRVKLKHYRTPGEITMKTPLLVTYALINRETMLDLQPDRSVVQTFLNSGV
                     DLYMIDWGYPTRKDRFLTIDDHVNGYMDNIVEFIREETGAPKINLMGICMGGTFCVIY
                     SALHPEKIKNLITTVTPTNFDTDKGLLHIWMRAIDTDKMINAFGNLPGDVMNIGFLLL
                     NPARLMIDKYVGFFENMGSKSFVENFVRMEKWIFDSPDVPGETFRQFASDFYKKNLLI
                     QNKLEIGGRHVDLKNITMPLLNFYGMYDHLVPPEACGMLTKCVGSTDTEDITLDTGHI
                     GIYVSSKCQREFAPKIVRWLRERDGEAKFPAAYTVDTAATAQPVMDALAVAAGKADEK
                     DKAGPRPRAKRVARASGRKQSAAKRQTVKPSLTQIIQDTI"
     gene            complement(7960..8868)
                     /locus_tag="PLT63_07060"
     CDS             complement(7960..8868)
                     /locus_tag="PLT63_07060"
                     /inference="COORDINATES: protein motif:HMM:NF021246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(R)-hydroxyalkanoic acid synthase subunit
                     PhaE"
                     /protein_id="HOD98518.1"
                     /translation="MEEKKSDQINLDAVMQDWMKASTDFWTSMLKAVPTAAPAFKVSE
                     VGEGIKNRYAESMEAALKRMQSVSTTFNDPQVADAVLKGLNTMPEFILKIVRASWEAS
                     AKIQKQAMEKAGKIGQKTEAYQFENLDQDVFKLWREIYEEEFRQYFQIPQLGLTRYYQ
                     ERMNRLADQANILQAALSEFFYLLYLPMEKSMQVLQDQVEELSKEGKVPEKAKEYYNL
                     WVKILEGHYMNLFKSPEYLAAFREMLDQVENFANAKNEVLQDLLQSLPVPTNRDMDNI
                     YKDLYLLKKRVRVLEKELGAKSKEKK"
     gene            complement(9089..9568)
                     /locus_tag="PLT63_07065"
     CDS             complement(9089..9568)
                     /locus_tag="PLT63_07065"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOD98519.1"
                     /translation="MAATRVFIHGLESTSQGTKGVYFRESYPGMIIEDYTGSLEERMT
                     KLEAVLAVKEDLILVGSSYGGLMAAIYACRHPERVRRLILLAPAINLEEFTPYTSHAL
                     TIPVHLYHGDQDDVVPTNEVWPVAEAVFSDLRYHLVEDDHSLHNTFPALPWEELLQD"
     gene            9857..11092
                     /locus_tag="PLT63_07070"
     CDS             9857..11092
                     /locus_tag="PLT63_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HOD98520.1"
                     /translation="MARIVPFRALRPDRHYAARIAALPYDVIDVEEAREIAGGNPWSF
                     LHVEKSEIDLPDYPHVEEGSVFQTAARNLEKMREKNILCQDPEPCLYVYAQEMAGRRQ
                     YGIVAAVSVREYEAGLIKKHEHTRADKEQERIGHVDAVSAQTGPVFIAYRKRGDIDAI
                     VSRIAQSPPEYDFVASDGIAHRVWVVADPAAGRLTAAFAQVPALYIADGHHRAAAAAA
                     VARRRRERDPVPSPDRPYESFLAVLFPHDQLRIMDYNRAVRDLHGLSGSDFLARISEN
                     FKVFADFSDRSPRRRREFGMYLDGTWRRLVFRKELPVSPDPVRALDVSILQDYLLGPI
                     LGIGDPRTDRRIDFIGGIRGMEELERLVDSRRFAVAFSMYPTTMGELMGVADTGLVMP
                     PKSTWFEPKLRSGLFVHLI"
     gene            11265..>11683
                     /locus_tag="PLT63_07075"
     CDS             11265..>11683
                     /locus_tag="PLT63_07075"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOD98521.1"
                     /translation="MAKTGRTYPTDNRLREGETVDELLGGRLWVIQERWGYRFSLDAL
                     LLANFVRMKNGASLLDLGTGSAVIPMLLCRRWACTRAVGVEIQGELADMARRSLELND
                     LTGAITIIEGDVRDVAKLVPFRSFDAVTCNPPYRKLRS"
BASE COUNT         2428 a         3098 c         3297 g         2760 t
ORIGIN      
        1 cggtcagaca ttccgccatg gccgccgatt cgtattgatt taccttgcat cccagggtgg
       61 cgatggccac cttgatgaat gccccggagg ccacgatggg gacttgagat gccttattgt
      121 tcgcatcatc atggtccgcc ggggtggcat caaaaacacc gcccgattcc ggtgtggaca
      181 tgtcggccgt ttccttcagc ataacatatt cttttctctg ctcttttccc tcatgggccg
      241 aaaagcataa ctgcggcccg attcttttgt caaggtgaaa ggatactgga tttttttccc
      301 gaattccgct aataagtacc taggtttctt tgtcccaaac ggaaggcatc catccatgga
      361 agcaatatgg tcttcaatcc atacccatca gtttgccgta gtggcgatcg tcatcgccgc
      421 cctgatcgtt atctattttc ttttcaaaaa cctcatcaag cttgccttgc tttttctcct
      481 catcatgatc ctgaccgggg gttatttcta ccttactgct ccgaagaaat ctcccgaaga
      541 cctcaaccgg gccatcgagc aaacccagga gcagaccgcc gagattatcg acaaggggaa
      601 agtcgcttat gagaagggta aggaggttgt agaacggggc aaagaactta ccgaccggtt
      661 cggcaaggtg attggcggca acccccgccg ggattgattc ctgcggcgtg aatatgccgg
      721 ccataccgcg gatattatgg tcttgggcgg cgaccggcgg ggcgggagaa tcaaaatatg
      781 ttgacctcca tggctcgcac cggacaggcg gagacgcata gttcgcaccc gttgcacttt
      841 tcgggatcga agaccacctt ccgagtgccg ggctcaaaag agagggcgtc ggtgggacag
      901 aaggccgtgc aggccccgca atgaacgcac ttgtcggaat tctggttgac gctcttggat
      961 aagggctcca ccttgagacc tttatccttg agaaaacgga tgccgttgtc gaaattccgc
     1021 tggttgccgc tcagctccag gactattatg ccttcccgct tggggaagat ccgggccttg
     1081 aggatgttga ataccaggtc atgggttttg acgagctggc agataatcgg ctggtcgacg
     1141 ttgtcggccg tgtagtgaat gacgattttt ctcgatacga tttgttcctc cgccatgatc
     1201 tcgctcccat cggaattggt tggggcgatg ttaggggagc ggtctttttt tgtcaaggct
     1261 ttcatgacga tcctttcatt tatctatcgg ttgcccttgc ctttcttgtc ctcttccccg
     1321 gcaccggccg ccgggggaaa gatatcgaag aacggcttga agggatttat ggggttgatc
     1381 gcggaataat cgatgcccaa atccagccac tttttgaact gattgtccgt cacggaacgg
     1441 taggataggt agttattgag gatctgttcc aggtattgct gaaaaaagtc gttgaggagg
     1501 ttttccccga accggatgag gaggtgcagg aggggtacgg ggagcagaaa attttttttt
     1561 ctcgattcct caagaatcac ctgggtgagg ataaaggcgg taacgtcctc cccggttttg
     1621 gcgtccagga cctgaacctg atggtccgca cggatggccc gggttatgtc ttcgaggttg
     1681 acataactgc tcttggccgt atcgtagagg cgtcggttgc cgtatttttt atagaggagc
     1741 ttttctgcca taagcgcttt ccttgtatgc aggatgtctt taagccttag caggtatcat
     1801 gttataacgc aacaaaaaaa actgtttatg ggcagaaaag tggttaagcg gaggctttta
     1861 atttgataac aactcgataa ataaaagatt attttatgac ggcggtgaat gccctgttta
     1921 tttgtgggca aaaattattt tgcaatgcaa catttttcct tgacaaacaa aaaaggaagg
     1981 ctataatagc ccaaaaacaa acaggaggta tcaaaaaaat ggaacaggga aagatagcca
     2041 aacagatgat cgattttcac aaggccacct tcgacaacac cttcaatgcc atgtcgattt
     2101 tacaggaaca gacggagcgg atggtgaaca tgtttctcga acagtctccc tgggtgcccg
     2161 aagaggggaa aaacgccatt caggactggg tgaaggccta caagaaggga cgtatggatt
     2221 tcaagacttc cgccgacgac agcttcaaga aggtggaaga ttttttcgcc gggttcaaca
     2281 agcaggccaa ggaaacaaag gtcaaagcgg gcaaatagcc tgaaataatt aaaaggagga
     2341 atgaatatgg atccgaaaca gatttccaaa cagatgttcg atttcaacaa gatggctttt
     2401 gagcggtcct ttgcggccat ggtggtcatg caggatcagg cggagaagat gttcaatatg
     2461 tggctcgacc agaacaagtg gttccccgag gaagggaaga aggccatgtc ggagtgggtg
     2521 aagacctaca agaagggtcg ggaagacttc aagtccaagg ttgaagaggg atacgacaag
     2581 atcgagcaat atctggcgcc ggcggaaaaa tagggcatgt agataaaatc gatttggttg
     2641 cagggaacaa ttatgatctc caagtatcaa caacaggttt tgaaacgtgc cgcgtcccta
     2701 tacacggaca ggttaaccga gacccccgga aagatgctgg gtgtagccac ggatatcctc
     2761 aaggccggcc agctttacgg cctgggcgtc atggcatatt ttgccgattt catggttccc
     2821 ttctggacgg cgtggcagag cttcaaccgc ctggaaaagg aaaagctccg ccggactttc
     2881 tttctccaga gcctggccga ctattacgag ttgatgcaat tcaatcttca aatcgccgag
     2941 caaggcatgc agggcacggt caacggtttt cgtgatttct accggaagga aaccgcccag
     3001 gccctcatgt ccctgctcaa cagcctctac ggggaggaag aagaggatgt gggcaagtat
     3061 gccgcacgtc tggttcaact taccgacggc gtcatcaatc gttaccccga ggcaatcaaa
     3121 aatatcggtg gcgaattcgg cttccacttc gacgacgacg gttacatcaa gacggcggag
     3181 acggaccggt tcttcctgta tcaggtcctt tcccgggata aaagcatcac cccccgcaag
     3241 gacgggaaac cggtcgtcat cattcatccc tatgtgctgg gaccgaatat cctggccttt
     3301 cttcccgatg aaaagaaaag ctatgtccat gcctttgccg atcagggcat ccccacctac
     3361 atccggatcc tgaaggatat ccagactacg gaggcggtgc agatcatgac gggggaagac
     3421 gacgccctcg atacccgccg gttctgtgaa ttgcttatgg taacccatgg ccgtcccgtg
     3481 accctgaacg gtttttgtca gggcggtttt atggcccttc tggatatcct ctccggcgaa
     3541 ctcgatggct tagtggacgc cctgatcacc tgtgtcgctc ccatggacgg cacccgcagc
     3601 agatccctgg tggaatacat gcagcatttg ccgggtcggt tccgggactt gaattatgcc
     3661 atcaagaagc tgccgaacgg caacggcatc gtggacggca aggtcatgag ctgggtctat
     3721 aaattgaaga gtatggagaa ggaggccccc ttcgtcactt tttaccggga cctgagcaac
     3781 gttggcgggg aagggatcag caagacggcg gcggcgatga accactggct gatctatgac
     3841 cggatcgatc tgccggaggg cattaccaaa ctgagtttcg attcctatac gattccggtg
     3901 accgaagacg gcaccctgcc ggtaaagctc ttcggcagga ccctaaattt caaggggata
     3961 caggaaaagg gcattccctg gctgctctgc tacgcggaac gggatgatct tgtggacaag
     4021 gacgctgccc tggcgcctgt ggatttcatc gacgtggaag taacgccttt tcccaagggg
     4081 cacggggcga tcgcgannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngatt
     4201 tcatcgacgt ggaagtaacg ccttttccca aggggcacgg ggcgatcgcg acctcctggt
     4261 ccgatccgcg ttccgcctgc gccctccaca cccggttcgg caatggttgc cggggacccg
     4321 tgcgattcca gctcgatctg gaggaaggga agacaacgaa caatggatcg taatttctac
     4381 gagttttggg gacagatgtt tctgaatttg gccaaggggc agaagcagat cgaggatttt
     4441 cgtaaatggt tcggccaggg gctgatgggt tatgagtcca tgaatgcgat gttccgcaag
     4501 gcatacggac tggaccggat cgaagaagaa tcgccggatt atgcggaaat ctggaagaag
     4561 tcggtggaga cctttcagaa atcctttcgg gagggcctat ccctgatgga ggtggttccc
     4621 aaaggagaat atgaaaaact cgcgcaaaag tgcgccgctc tggaaaaaac cgtggcggag
     4681 caggaggaga cgattcgtac cctgaagcgt ctcctcaccg aagaggcgag cggcgctccc
     4741 gccgccgccc tcgaggatct ccaggggctg ctcagagatc aacagaccca gtttcaggaa
     4801 ctggtgaaaa acatgacgct cctgttcgaa aaggagccgg tggtcacccg ggggaccaag
     4861 aagaaggggc actgactccc cgccgttttc cccgtcacgt tcggtccata ctgtttccta
     4921 actcggaacg aatcaatcat ccggctgcat tccctgttgc cggatcgata ttatcttcgc
     4981 gatgcgagtt ttgctacctg ataaggcgcc ctatggggcg tggatcgctc ttcaattccg
     5041 gtggttcatc atgtggcggt ttcaggcctt gggagggctt acgagcgcct cgccgtccag
     5101 gacgacggtt ccctcctgat tccggcaggt ggtttggagt cggagcctgt ttttctccgt
     5161 gattacctcc acgacctcta cccgcgcggt gatcgtgtcc cccatgcgga ccggggcgag
     5221 aaatttcaag gactggctca tatagatcgt tcccggaccg gggagttgca tacccaggat
     5281 ggccgagatg aagcccgccg tcaatatccc gtggacgatg cgacccttga aaaacgtccc
     5341 ggcggcatag gtctcgttga tatgggccgg attcaaatcc cccgtgatcc cggcgtagag
     5401 atagacgtcc gtttccgtga cggtcttggt aaactccgcc gcgtccccca cctttatcgt
     5461 atcaatggtt cgtcccttca tcttcctttc ctcccgtctc gattgtttac ccggcggcgg
     5521 actttcgccc ccgccgttcc gcatgaacat cgggcacttt cctcccggcg gttaaaacca
     5581 gcatttgtgg gtccaggtcg cctctttcat gtccctcaga tcgttctgca acatgagata
     5641 catgctggca ttatggatgg ccagcccccc ctgataggcc agagcggtaa cgagtccgat
     5701 ctcgtcctcc gtgaattccc gcttcttgtc gcagtaaaga cgcagaaccc cgatgacctc
     5761 gttcttggcc ttgatcggta cgcagaggat ggagacgatc ccctcctctt tcttttcctt
     5821 cttgtattgc acccccttgt ccgtataggc atcggtaatg gccaccggtt tgccgttgag
     5881 ggcttcagcg atgctcttct cggcagaaat aggacctttt ttcaaatact tgtcgctcaa
     5941 accatagctc gccaccagtt gcagcaccgt tctttcgtcg ttgaggagac ggatcgaggc
     6001 cgcttttacc cgcagggcct tggccacttc ctcggtgagg gtttgcagaa tcgtcttgat
     6061 atccagactg gcggcgatat cggcggcgag gttcaggaag agtttcgtgt tgtcccgcag
     6121 ttgatcgaca agtttcttga tctggatggc ccagccgccc tggccggcca ggagttgcag
     6181 gaattcaatc tcgtctctgg tgaattccct gatttttgat gtgtaaagag tcaggatggc
     6241 aatgagttca cctttgacca tggcaggaac gacgataatg gaaccgatgc cttccgcttt
     6301 cttgccctcc cggttttcca ggcggatgtc cgtcgtggca tccttgtagt agatgaagcc
     6361 atcctgcttc agaatgggga tgatcttttc ggcgtgtccg taaccggcat ggagatagtc
     6421 ggcggacagg ccacgctggg cgacggcctt gaagatttcc cgtccctcat ccgccaaaaa
     6481 gaggctggcc gcctttgcct tcatggtccc aatggcgctg tccacgatca actgcaacag
     6541 ggcgtccata tcggtggtgg tgccgaaggc gtcactgact ttgatgaacg tctttaaata
     6601 actggtctgc ttttccggca ttttcctttc ccccctttaa atcgtatcct ggatgatttg
     6661 tgttagcgat ggtttgacgg tctgcctttt cgccgctgat tgttttctcc ccgacgcgcg
     6721 ggcgaccctc ttcgcccttg gcctcggacc ggctttgtct ttctcatcgg cctttcctgc
     6781 cgcgacggca agggcgtcca tgaccggttg cgccgttgcg gccgtatcca cagtgtaggc
     6841 cgccgggaat tttgcttccc cgtcccgttc ccgaagccac cggacgatct tgggggcaaa
     6901 ttcccgctgg cacttggagc ttacgtagat gccgatatgt cccgtgtcca gggtaatatc
     6961 ctccgtatcg gtgctgccga cacatttggt aagcatgccg catgcctccg ggggaacgag
     7021 atggtcgtac attccataaa agttgaggag cggcatggtg atgttcttca aatcgacgtg
     7081 ccggccgccg atttcgagct tattctggat caggaggttc ttcttgtaga agtcggaggc
     7141 aaactggcgg aaggtctcgc cgggaacatc ggggctatcg aagatccatt tttccatccg
     7201 gacgaagttt tccacaaagg acttgcttcc catgttttcg aagaagccca catatttatc
     7261 gatcatcaac cgggccggat tcaggagtag gaaaccgatg ttcatcacgt ctccgggcag
     7321 attgccgaag gcgttgatca ttttgtccgt gtcgatggcc cgcatccaga tgtgcagcag
     7381 ccccttgtcc gtatcgaagt tcgtcggcgt aacggtggtg atgagatttt tgatcttctc
     7441 cggatggagg gcggagtaaa tgacgcaaaa cgtgccgccc atgcagatgc ccatgagatt
     7501 gatcttggga gcgcccgttt cctcccggat gaactcgacg atattgtcca tgtagccgtt
     7561 gacatggtcg tcgatggtga gaaaacggtc cttcctggtc gggtaacccc agtcgatcat
     7621 gtagaggtcg acgccgctgt tgaggaaggt ctggacaacg ctccgatccg gctggagatc
     7681 cagcatcgtc tcccggttga tgagggcgta cgttaccagc aggggcgtct tcatggtaat
     7741 ctccccgggg gtgcggtagt gcttcagctt gacccggtcc tcttcgtaaa ccacatcgta
     7801 gggagtcatg gccaagttgg attccagtgg gcccaggagg acctccgacg ctttggtgac
     7861 tgtttcctgc gccttttccg cctccgaggc gatattggct aaaatgagat ccagggatat
     7921 cttgggttgt tccattgtcg tcacctcctt caataggggt tatttctttt ctttactttt
     7981 tgcgcccagt tccttttcga gaacccgcac gcgtttcttc agcaggtaga gatccttgta
     8041 gatgttgtcc atatcccggt tggtgggcac gggtagggac tggaggagat cctgcaagac
     8101 ctcgtttttg gcgttcgcga agttttccac ctggtcgagc atctcccgga aggccgccag
     8161 gtattccggg gatttgaaga ggttcatgta atgcccttcg aggatcttta cccaaagatt
     8221 gtaatactct ttggcctttt ccggcacctt tccctccttg gatagctctt ccacctggtc
     8281 ctggaggacc tgcatggatt tttccatcgg cagatagagg aggtagaaga actccgacag
     8341 ggcggcctgg aggatgttcg cctgatccgc caggcgattc atccgttcct gataataccg
     8401 ggtcaatccc aattgcggga tctggaaata ttggcgaaat tcctcctcgt atatttccct
     8461 ccagagcttg aagacgtcct gatccaggtt ctcgaactgg taggcctcgg ttttctggcc
     8521 gatcttgccg gccttttcca tcgcctgctt ctggatcttc gccgacgcct cccacgacgc
     8581 ccggacaatc ttcaggatga attccggcat ggtgttgagc cctttgagta cggcatcggc
     8641 aacttgcgga tcgttgaacg tcgtcgagac ggactgcatc cttttcaggg ccgcttccat
     8701 tgattcggcg taacggttct tgattccctc cccgacttcg gataccttga aggcgggtgc
     8761 ggcggtgggg acggccttga gcatggacgt ccagaagtcc gtggaggcct tcatccagtc
     8821 ctgcatcacg gcgtcgagat tgatctggtc cgactttttc tcttccatat tggacacctc
     8881 aaaaaataaa gaaagctgat aaggctgtcg cctgttaata atcggggggt atgattgaaa
     8941 gctgtaccct ataatatgat aaaaaagcct ccagtgtcaa tagtggttaa ttctaatgaa
     9001 cggcggtttt cctacttttt taagctggca tcctgttttg tattgtatcg gtcaaggatg
     9061 cgaagatacc ggcgtccctg acgccgtttc aatcctgaag cagctcctcc cagggcagcg
     9121 cgggaaaggt gttatggagg gagtggtcgt cttccacgag gtggtagcgg agatcggaaa
     9181 agacggcttc cgccactggc cacacttcgt tcgtggggac cacgtcgtcc tgatctccgt
     9241 gatagagatg gacgggaatg gttaaggcgt gactcgtata gggggtaaat tcctctagat
     9301 tgatggccgg ggccaggagg atcaagcggc gaacccgctc cggatggcgg caggcgtaaa
     9361 tagccgccat aagccccccg taactggagc ccacaagaat caggtcctcc ttcactgcca
     9421 ataccgcttc cagcttcgtc attcgctctt cgagcgaacc ggtgtaatcc tcgatgatca
     9481 tcccggggta cgattcccgg aaatagaccc cttttgtacc ctgactggtg ctttccagcc
     9541 cgtgaatgaa aaccctcgtt gccgccatga cctgcctccc tgttttcagc ccgacgcctg
     9601 tccgctccgg gccacggatt ctttgccgtt cccatatcat ctttgccgtc aaacgggaat
     9661 agctacttgt ggcgattccg gaccgattcc ggacctgacg cgggattgga gccggcgcca
     9721 atagcgacgt atgtaatcga ggacattgtg ccgccgggac aaagatttcc gggttcggta
     9781 tcccttccct cacgttgctt tcggataacg agtgtggtag aaatcccgga gcgaaaacat
     9841 tggaaaggtt tcattcatgg cgcgtattgt cccctttcgg gccctccgtc cggatcgtca
     9901 ttatgccgcc cgaatcgcgg ctctccccta cgacgtcatc gacgtggagg aggcccggga
     9961 gatagccggc ggcaatccct ggagttttct ccacgtggaa aaatcggaga tcgatcttcc
    10021 ggactaccct catgtcgagg aggggtcggt ctttcagaca gccgcccgga atctggagaa
    10081 gatgcgggag aaaaatatcc tctgccagga tccggagccc tgtctgtacg tttatgccca
    10141 ggaaatggcc ggccgtcgcc agtacggcat cgtcgccgcc gtcagcgttc gggaatacga
    10201 ggcggggctg atcaagaaac acgaacatac ccgggcggac aaggagcagg agcggatcgg
    10261 acacgtggat gccgtcagcg cccagacggg cccggttttt attgcctacc ggaagcgagg
    10321 ggacatcgac gccatcgtaa gtcggatcgc ccaatccccg ccggaatacg atttcgtcgc
    10381 ctccgacggc attgcccacc gggtctgggt cgttgcggat ccggcggcgg ggagattgac
    10441 ggccgctttc gctcaggtgc cggcgcttta cattgccgac ggacatcacc gggcggcggc
    10501 tgccgccgct gtagcccgga gacgccggga gcgggatccg gtcccttccc ctgatcgacc
    10561 ttatgaatca ttcctggcgg tccttttccc ccacgaccag ctccggatca tggattacaa
    10621 ccgtgccgta agggatctcc acgggctttc cggcagcgat tttttggcgc gaatttccga
    10681 gaatttcaag gtcttcgccg atttctcgga ccgttccccc cgccgccgcc gcgaattcgg
    10741 catgtatctg gatggcacat ggcggcgcct cgtcttccgt aaggaattgc cggtttcccc
    10801 ggacccggtg cgtgccctgg acgtttctat cctccaggac taccttctgg gcccgattct
    10861 cggcatcggc gatccccgga cggaccggcg catcgatttc atcggcggga ttcgggggat
    10921 ggaggaactg gaaaggcttg tagattcccg gcgctttgcc gtggcctttt ccatgtaccc
    10981 cacgaccatg ggggagctta tgggggttgc cgacaccggt ctggtcatgc cccccaaatc
    11041 gacctggttc gaaccgaagc tccgcagcgg tctgttcgtt cacctcatct gaagggttcc
    11101 gacggaggcg acgatgggat ggggcggcgc tttccgggag ccggtttcgt tgttgcgcgt
    11161 ccggatcggc ggacctttgc cgccggtgcc cggatcacca ccggagggga tggatgaaag
    11221 acagccggcc gcgttgagcc gggaaatcaa tgggaaggct ggggatggcg aaaacgggcc
    11281 gtacgtatcc gaccgataac cggcttaggg agggggaaac ggtggatgaa ctcctgggag
    11341 ggcgtctgtg ggtcatccag gagcgctggg gctatcgctt ttccctggac gccctactgt
    11401 tggccaattt cgtccggatg aaaaatggcg cctccctact ggatctgggg acgggaagcg
    11461 ccgtgatccc catgctcctg tgccgccgtt gggcctgtac ccgggccgtg ggggtggaga
    11521 tccagggcga actggccgac atggcccggc gaagtctgga gctcaacgat ctcacggggg
    11581 cgattaccat cattgagggc gatgtccggg acgtggcgaa gttggtcccg ttccgatcct
    11641 tcgatgccgt gacctgcaac cccccctacc ggaagttgcg ctc
//
LOCUS       DAOSMF010000061        11248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_6642_length_11248_cov_19.171178, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000061 DAOSMF010000000
VERSION     DAOSMF010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 11248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11248
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6642_length_11248_cov_19.171178"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="PLT63_07080"
     CDS             <1..229
                     /locus_tag="PLT63_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98522.1"
                     /translation="VAGRWLVFIAAPLFGVFSRQMTRKIGREAQALFAGVLEIEEVST
                     CSRDHGSLGGEKHRPECGTAREERTGKVYDL"
     gene            251..655
                     /locus_tag="PLT63_07085"
     CDS             251..655
                     /locus_tag="PLT63_07085"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HOD98523.1"
                     /translation="MQAEHIVWDPKFSIHIDEIDAQHRELFAAVNELIDLLAGSGGEL
                     YPVMKKLVDYLSRHFRAEQKLMIETGYPYYARHVGEHRLFTDKVEEFLGKYKEQDKSL
                     IQAMVLFLRHWVYSHTTTIDMKFADFLKDRAK"
     gene            685..1134
                     /locus_tag="PLT63_07090"
     CDS             685..1134
                     /locus_tag="PLT63_07090"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOD98524.1"
                     /translation="MEAETRLIAGDLIIQEDCGNIDWDAVRGCLRAVGMAYRDPELHR
                     RAFLNSFAVVFLRNGEQLIGFGRAISDGAYQAAIYDVAVSPPYQRRGLGMLIMERMLA
                     RISHCNVILYANPGREDFYLRLGFRPMRTGMAKYLKPAVMKERGLIS"
     gene            complement(1184..2737)
                     /locus_tag="PLT63_07095"
     CDS             complement(1184..2737)
                     /locus_tag="PLT63_07095"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOD98525.1"
                     /translation="MYTAEFTIYDLIARNASVHARREAVTGGGYPLSFGEFHHRCDAF
                     VAGLRGEGLAAGDRIALLAGNEPDFLLLCVAAARLGAIVVPVNWRLGSEEIVYILADT
                     APRFLFCGREFRDQARRAATGMPSLEKRFVFAGDGEEATTAETGSDDFLTWPAEDFTA
                     ANVSLSPVAGRGADPFLIIHTAAMGGKPRGCILSQDNLMATAVQLAHLLRLDDRDAYV
                     GLLPLFHIGGMAMTLATMLAGGRNILVPRFDPPAVLRIIAASGGTFFGTFPPMLGALL
                     DAAEKGEYDTAGLRLACGVDAPATIERFLRVHPRAEFFSLYGQTEVMPVSGGNYRDCP
                     GGIGRPSLLARVAICDDMDEEVPPGTTGEICVRSPAVFRGYWRLPGETAAAARSGRHH
                     TGDLGRLDEEGFLWYAGRKPEKELIKTGGENVYPAEVEKAILAHQAIAETCVFGVPDP
                     EWGEAVRAVCVLAEGARVSAGELGDFVAARIARYKRPKYVIFVTALPKTPAGTVDREA
                     VKRNWGTKA"
     assembly_gap    3046..3145
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3164..3862)
                     /locus_tag="PLT63_07100"
     CDS             complement(3164..3862)
                     /locus_tag="PLT63_07100"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HOD98526.1"
                     /translation="MEKIDPRKYRRIVFFTGAGMSAESGVPTYRGRGGVWSQYRWEEF
                     ACQEAFDADPEKVLQFHELRRAAVLACAPHAGHAAVAALEKSHPGVTVVTQNIDGMHQ
                     RAGSGKVIELHGSLWHLRCPEHGITEDIGEKYRRFRCEQCGNWLRPDIVWFGDMLDQY
                     VMSAAIAAIRSCDLFVSIGTSGVVYPAAGFPSFAKGNEARCIEINPEANVISSLYDEA
                     IREPAGKALPELFA"
     gene            4015..5133
                     /locus_tag="PLT63_07105"
     CDS             4015..5133
                     /locus_tag="PLT63_07105"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOD98527.1"
                     /translation="MKRFIVFLSVFSLLACFAAAPALAADNIKVGAAFALSGSIAVYG
                     EGFKKCVDLAVEEINAKGGIKGKKIEIVYEDNKSNPKDCVSAVRKLISVDRVPVIFGP
                     AGSSNFMAAAPVAQESRVVMLSAQGTAEKLTQAGKYIFRIIPSNSKQGQRLAKLAFDQ
                     GYRRLPVMYINNDWGLDLKDGFVTSFRKMGGVVTDIIPFDEKKTDYRTELLRAAKGNP
                     RAIVNLTYINEGAVQFKQAYELGIKTQWLCGSAARAPKMVELAGKAAEGVMGTYPSVP
                     QDTKAYQAFKGAYKKKYGNDKIPIFGDYNYDMVYLTARAIEKGGYTAEGIRKALPAVS
                     KGYYGVTGDKTFDRQRGMEKADIGIWIVKGGKIEDYKK"
     gene            5162..>5935
                     /locus_tag="PLT63_07110"
     CDS             5162..>5935
                     /locus_tag="PLT63_07110"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOD98528.1"
                     /translation="MDSLPYYLQTIINGLLAGCIYALFAMGLTLIYGVLNFVNFAHGE
                     LIMWGAYFLLLALAEPFALPLYAAVPVALGMTALLGAGMDFAVFRHLRRSNRLTLLIA
                     AIGLSFFLRNAAQLFWGAEVRTYGFEIRPGLRWFGVSLTPQQLVIIVISFLCIVGVYL
                     LFFKSRIGKSMRALADNLDLARISGIHTGKAILWAWIISSLLAGTGGILMAMDTNLHP
                     EMGVANLIKAFAATLIGGVGNLWGALIGGLIIGLAENIAV"
     assembly_gap    5936..6035
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6036..6434
                     /locus_tag="PLT63_07115"
     CDS             <6036..6434
                     /locus_tag="PLT63_07115"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOD98529.1"
                     /translation="DDETAAAILGKNPFAYKMKSFVIGSLFAGLAGVLWAHFITFISP
                     GDFTLTETIVVLLIVVLGGKGTLWGPVVGAAVLIFFQESIRLLRLPPEWSGILGPLQQ
                     MVFGLLLVILMIFRPEGLIREKGKETFARD"
     gene            6421..7167
                     /locus_tag="PLT63_07120"
     CDS             6421..7167
                     /locus_tag="PLT63_07120"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD98530.1"
                     /translation="MLEIKNVTKSFGGTQAVAGCSLSLAGLKIAGLIGPNGSGKTTLF
                     NLITGLIRPDAGEIFFQDRRIDGGMPHRLAAMGLIRTFQLSRVFPRLTVMDNMLLAPK
                     GQAGERLRSLLLRFPLVRRQEGEHREKACELLDMLGIIHLQDQFAGNLSYGQQKLLEL
                     GRAVMAEPQLILLDEPTAGINPRLIRTMIDMILKMQDQGRTFFIIEHNMDVAAELCEH
                     IFVLDHGERIASGSPREIQNDPRVIEAYLG"
     gene            7173..>7651
                     /locus_tag="PLT63_07125"
     CDS             7173..>7651
                     /locus_tag="PLT63_07125"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOD98531.1"
                     /translation="MEGKDIYAGYGKIEVLHGASIRAGEGEIVCIIGPNGSGKSTLLK
                     ALFGLITPRRGKVLFAGRDVTHLAPEEKVRRGIAFIPQGRNVFTSLTVRENLEMGGTP
                     LGDEREVARAIGEVLESYPVLKDKLHHRAGNLSGGEKQLLALARADMIKPRMILMDEP
                     "
     assembly_gap    7652..7751
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7752..8455
                     /locus_tag="PLT63_07130"
     CDS             <7752..8455
                     /locus_tag="PLT63_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98532.1"
                     /translation="IGGAFATDSRGRIFVVHRGKLGGSRKGVGKALFEKRYRGVWMEM
                     DDGGEEASVAVVGVLPSPRFARQVAQFVGKIEKIKDMAAPSAQTEIGFDDLRLREELL
                     GTRRCELFRDTAGECDHGLVVKDLAAALKIIGCKFGNDGLRDLLALDPRGEVTIFQIK
                     TDNLYKSIQEGATRLLVNSLRATRPPRLVLVLPAAPDTGKEERLRRLNIDVLTYRWEG
                     NHAVFPGLETFLDIP"
     gene            complement(8632..10359)
                     /locus_tag="PLT63_07135"
     CDS             complement(8632..10359)
                     /locus_tag="PLT63_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOD98533.1"
                     /translation="MRRHLTMTRLRRLSQALFLLLFLWLFLQTESQGANELGYPVKIF
                     LDADPLLWITTALASRSFYGAFALALTVIVATILLGRIFCGWICPLGTLHQLAGKLKR
                     ERSAARRKGGGSITVVRLLEGLRGRRNAVSRRHNSVTGAGSTGSRQETIAGIEVPRRR
                     VRNWGQYRIKYLLLVFILVLSMLGVQAAGLFDPLTLLIRSLSLSVYPFLSYALRSIFT
                     MLYEWHIPLVSSGAEVVYELLKRSVLPFSQPLFFQGTAIGLLFFALLALNLRESRFWC
                     KYLCPLGALLGLLSRFALLKRSVSEGCNDCGRCRRECQGGAIGAKGAEWVAAECLYCF
                     NCDDPCPQHAVSFGFSSRPASFSLDLGRRRVLGAAVAGALTAPLLRITPLARAGAAEP
                     TLIRPPGALAEEEFLKRCVRCGECMKVCITGGLQPTLLEAGLEGIWTPVLVPRIGCCE
                     YRCTLCGQVCPTGAIGRLGLREKAATKIGLAMIDKGRCLPFAHDMSCIVCEEVCPTSP
                     KAIWLEEVRVRDRRGREMLLKQPHVDLELCVGCGICENKCPVLGRPAITVTSLGESRS
                     RDNQLLLVP"
     gene            complement(10372..>11248)
                     /locus_tag="PLT63_07140"
     CDS             complement(10372..>11248)
                     /locus_tag="PLT63_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOD98534.1"
                     /translation="AVLSGAALALPLGADSLRSLAEAAGRPDLAVARGVPPARITRAA
                     VAELGGMGRFVSRGDIVVVKPNIGWDRTPEYAANTNPEVVAELVRLCYEAGAKRVKVF
                     DFPVNDARRTYRQSGIAAAATAAGAQVSYIDDRKFKDTALRGQLLKSWPLYTEILAAD
                     KVINVPIAKVHGIATLTLGMKNWMGVMGGWRGRIHQRIDESLVDIARVIRPSLIVLDA
                     VRILVDNGPQGGSLADVRRLDTVIAGTDQVAVDAFGTTLFGMKADTLDCVRIGHRAGL
                     GNMDLSRLLIKRVSV"
BASE COUNT         2184 a         3446 c         3347 g         1971 t
ORIGIN      
        1 ggttgccgga cggtggctgg tcttcatcgc cgccccgctc ttcggcgtct tctctcgcca
       61 gatgacccgg aagatcggcc gtgaagccca ggcgctcttc gctggggtcc tggaaataga
      121 agaagtttcc acctgttcca gagaccacgg ctccctgggc ggggaaaaac accgccccga
      181 atgcggcacg gcccgggaag aaagaaccgg caaggtttac gatctttaat aatgcggagg
      241 ccgtaaagac atgcaagcag agcatatcgt ctgggacccc aaattcagta ttcatataga
      301 cgaaatcgac gcccagcacc gggaattgtt cgccgccgtc aacgagctta tcgatctgct
      361 ggccgggagc ggcggagagc tttatccggt aatgaaaaag ttggtggact atctgtcccg
      421 gcattttcgc gccgaacaga aattgatgat cgaaacggga tacccctatt acgcccggca
      481 cgtcggggag caccggcttt ttacggacaa ggtcgaggag tttctgggca aatacaagga
      541 gcaggataag agtttgatcc aggctatggt gctctttctc cggcactggg tttattccca
      601 taccaccacc atcgacatga agttcgccga tttcttaaag gacagggcca aatgatcctc
      661 ggcgccggag accaaggcgg catcatggag gcagagactc gcctaatcgc tggggatctg
      721 atcattcagg aggattgcgg caacatcgac tgggatgcgg tgcgcggatg cctgcgcgcc
      781 gtgggtatgg cctatcgcga tccggaattg catcgccggg ccttcctcaa cagcttcgcc
      841 gtggtatttc tccgtaacgg ggaacaactg atcggtttcg ggagggcgat ctccgacggg
      901 gcttatcagg cggcgatcta tgacgttgcc gtgtcgccgc catatcagcg ccgggggctg
      961 ggcatgctga tcatggaaag aatgctcgcc cggatctccc actgcaatgt catcctctat
     1021 gccaaccccg ggcgggaaga tttttatctg agattgggat tccgccccat gcgcacgggg
     1081 atggccaagt atttgaagcc cgccgtcatg aaggaacgag gcctgataag ttgaatgcgg
     1141 cgggtcggag gcgaatacct atgcccgccg gtggtgaagt cggtcaagcc ttcgttcccc
     1201 agttcctctt caccgcctcc cggtcaacgg tacccgccgg cgttttgggc agcgccgtga
     1261 cgaagatcac atacttcggc cttttgtagc gggcgatccg ggccgccacg aagtccccca
     1321 gttccccggc gctcactcgg gcgccctccg cgaggacgca aaccgcccgc acggcctccc
     1381 cccattccgg atcgggaacg ccgaagacgc acgtctcggc tatcgcctgg tgggccagga
     1441 tggccttttc cacctcggcg ggatagacgt tctcaccgcc ggtcttgatg agttccttct
     1501 ccggtttccg gcccgcgtac cacaggaagc cttcctcatc cagacggccc agatcgccgg
     1561 tatgatgccg gccgctccgg gcggcggccg ccgtttcccc gggcagacgc cagtagccgc
     1621 ggaatacggc gggggagcga acgcagatct ccccggtcgt ccccggcggg acctcctcgt
     1681 ccatatcgtc gcagatggcg acccgggcca gcagggacgg acggccgatg ccgcccgggc
     1741 aatcccggta attgcccccg gaaacgggca tgacctcggt ctggccgtag aggctgaaga
     1801 attccgcccg gggatggacc cgcaggaatc gttcgatggt cgccggggcg tccactccgc
     1861 aggccaggcg caggcccgcc gtatcgtatt cccctttttc cgccgcatcg aggagcgcgc
     1921 ccagcatggg gggaaaggtc ccgaaaaagg tgccgccgga tgcggcgatg atacggagga
     1981 cggcgggagg gtcgaatcgc ggtacgagga tgttccggcc gccggccagc atcgtcgcca
     2041 gggtcatggc catcccgccg atatggaaga ggggcaggag acctacatag gcgtcccggt
     2101 cgtcgaggcg gagcagatgg gccagttgca ccgccgttgc catgaggttg tcctgactca
     2161 agatacatcc ccggggtttg ccgcccatcg ccgccgtatg aatgatgagg aacggatcgg
     2221 caccgcgccc ggcgacggga gacaatgaga cgttcgcggc ggtaaaatct tccgccggcc
     2281 aggtgaggaa atcatcactg ccggtctccg cggtggtcgc ttcttctcca tcgccggcaa
     2341 agacgaagcg cttctccagg gaaggcatcc ccgttgcggc ccggcgagcc tggtcccgaa
     2401 actcccggcc gcagaacagg aaccggggtg cggtgtcggc gaggatatag acgatctctt
     2461 ccgaccccaa tcgccagttc accggcacga cgatggcgcc gagccgggcc gccgccacgc
     2521 agagcaggag aaagtccggc tcgtttcccg ccagaagggc gatccggtcc ccggcggcaa
     2581 gtccctcccc ccgcaaaccg gcgacaaagg cgtcacagcg gtggtgaaat tccccgaagc
     2641 tcaggggata gccaccgccc gtcaccgcct cccggcgcgc atgcacggac gcattgcggg
     2701 caatcaggtc ataaatcgta aactcggcag tgtacatggt cgctccctta cgatgtcttt
     2761 atcatgaaaa cacatccata cgatctcact taaaccattt tcatcgtgcg cgggtgttgg
     2821 acaaaaatga taattcatat gaaaaagcct ctatatgctt tactccatat caggacacca
     2881 tcatgtgcgt ttcatgcacg tcaggcttac ccagccacca cttccgcccg cgcggatttc
     2941 tctttcacgg cggtgccgcc tctataattc acccggcggc cgatcaggcg acgaaacaac
     3001 cggggctcct aagcaaagag ttccggaagc gccttccccg ccggcnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnacgaa acaaccgggg ctcctaagca aagagttccg
     3181 gaagcgcctt ccccgccggc tcccggatcg cctcatcata aagggaggat atcacgttgg
     3241 cctcgggatt aatttctatg caccgggctt cgttgccctt ggcgaagctg ggaaagcccg
     3301 ccgccggata gacgacccca gaggtcccga tgctcacgaa gagatcgcag gaccggatcg
     3361 ccgcaatggc ggcgctcatc acgtactggt ccagcatgtc tccaaaccag acgatatccg
     3421 gtcgcagcca gtttccgcat tgctcgcagc ggaaacgccg gtacttctcg ccgatatcct
     3481 ccgtaatgcc gtgctccgga cagcgaaggt gccagagact cccatggagt tcgatgactt
     3541 ttccacttcc cgcccgctgg tgcatcccgt cgatgttctg ggtcaccacc gtcaccccgg
     3601 gatggctttt ttccagcgcg gcaacggcgg catgtccggc atggggggcg caggccagga
     3661 ccgccgcccg gcggagctca tgaaattgga gcaccttttc cggatcggcg tcgaaggcct
     3721 cctggcaggc aaactcctcc cagcgatact ggctccagac gccgccgcgg ccccggtagg
     3781 tggggacgcc gctctccgcc gacatcccgg cgccggtgaa aaagacgata cggcgatatt
     3841 tccggggatc aattttttcc atgtcttcac ctcccgatga tctgcctgat tagcacggcc
     3901 cttgattctc cgcaaccccc atggtgaatc tcattgtcac cggccctttt tcattgacag
     3961 ggccggacat taactatagg aaagatgcct caataaatag cgggaggtcg catcatgaaa
     4021 agattcatcg tttttctatc ggtattttcc ctgctggcat gcttcgccgc cgccccggcc
     4081 ctggcggccg acaacatcaa ggtcggcgcc gccttcgccc tttcgggaag catcgctgtt
     4141 tacggcgagg ggttcaagaa gtgcgtggat ctcgccgtgg aggaaatcaa cgccaaggga
     4201 ggcatcaagg gaaagaagat cgagatcgtc tatgaggaca acaagagcaa ccccaaggat
     4261 tgcgtatcgg cggtacggaa gctgatctcc gtggacagag tccccgtgat cttcggcccg
     4321 gcgggcagct cgaacttcat ggccgcggcc ccggtggccc aggaaagccg ggtggtgatg
     4381 ctgtcggccc agggcacagc ggaaaagctt acccaggccg gcaaatacat tttccggatc
     4441 atcccctcca attccaagca ggggcagcgc ctggcgaaac tggccttcga tcaaggttat
     4501 cgccggcttc ccgtgatgta catcaacaac gactgggggc tggaccttaa ggacggcttc
     4561 gttacaagct tccggaagat gggtggcgtc gtcacggata tcatcccctt tgacgagaag
     4621 aaaacggatt accgcaccga gctcctgcgg gccgccaagg gcaacccccg ggccatcgtc
     4681 aatctgacct acattaacga aggggcggtg cagttcaagc aggcttacga gctgggcatc
     4741 aagacccagt ggctctgcgg gtccgccgct cgggccccca agatggtgga gctggccggc
     4801 aaggccgccg agggcgtcat gggaacctat ccctcggttc cccaggacac caaggcctac
     4861 caggccttca aaggcgccta caagaaaaag tacggcaacg acaagatccc catcttcggc
     4921 gactacaact atgacatggt atatctgacc gcccgggcca tcgaaaaggg cggctacacg
     4981 gcagagggga tccgcaaggc tctaccggcg gtttccaaag gctactacgg cgtgaccggg
     5041 gacaagacct ttgatcggca gcggggaatg gagaaggccg atatcggtat ctggatcgtc
     5101 aaaggcggca agatcgagga ctataagaag tagcagccgg agcggtttct cctttaacag
     5161 catggattcc ctcccctact acctgcagac gatcatcaac ggccttctgg cgggctgcat
     5221 ctatgccctc ttcgccatgg ggctgaccct gatttacggg gttctcaact tcgtcaactt
     5281 cgcccacggc gagttgatca tgtggggggc ctattttctc ctcctggccc tggcggaacc
     5341 tttcgccttg cccctatacg cggccgtccc cgtagccctg gggatgacgg cgcttttggg
     5401 ggccggcatg gatttcgctg tgttccggca cctacggcgg tcgaaccggt tgacgctgct
     5461 gatcgccgcc atcggcctgt cctttttcct ccgcaacgcc gcccagcttt tctggggggc
     5521 ggaagtgagg acctacggat tcgagatccg gccggggctc cggtggttcg gtgtttccct
     5581 caccccgcag caactcgtca tcatcgtcat ttcgttcctt tgcatcgtcg gcgtttactt
     5641 actcttcttc aaatcccgga tcggcaaatc catgcgcgcc ctggccgaca atctggacct
     5701 ggcgcggatc tccggcatcc ataccggcaa ggcgatccta tgggcctgga taatctcgtc
     5761 gctccttgcc gggacggggg gaatcctgat ggccatggac accaacctcc atcccgagat
     5821 gggggtggca aacctcatca aggccttcgc ggcaaccctc atcggcgggg tgggaaacct
     5881 ctggggcgcc ctgattggcg ggctgatcat cggtctggcg gaaaacatcg ccgtcnnnnn
     5941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngacga cgagacggcg gcggcgatcc
     6061 tcggcaagaa cccttttgcc tacaagatga aatcctttgt catcggctcc ctcttcgccg
     6121 gtctggccgg cgtcctctgg gcccacttca tcaccttcat cagccccggg gattttaccc
     6181 tcaccgaaac catcgtcgtt ttgctcatcg tggtgctcgg gggaaaaggg actctgtggg
     6241 gtcccgtggt cggggcggcg gtgctgatct tttttcaaga atcgatccgg ttgctgcgac
     6301 tgccgccgga atggagcggc atcctgggac cgctgcagca gatggtcttc ggcctgctgc
     6361 tggtaatcct gatgatcttc cgtccggaag gcctgatcag ggaaaagggg aaggaaactt
     6421 ttgctcgaga ttaagaacgt cacgaaatcg ttcgggggga cccaggccgt ggccggctgc
     6481 tccctcagcc tcgccggact gaagatcgcc gggcttatcg gccccaacgg ctccggcaag
     6541 acaactcttt tcaacctcat caccggcctg atccgtcccg atgccgggga aatattcttt
     6601 caggaccggc gcatagacgg cggaatgccc caccgtctgg ccgcaatggg actgatccga
     6661 accttccagc tatcccgggt cttcccccgc cttacggtga tggacaacat gctgctggcc
     6721 cctaagggac aggcggggga acgcctccgg tccctgctgc tccgtttccc cctggtccgc
     6781 cggcaggaag gggagcaccg ggaaaaggcc tgcgaacttc tggatatgtt ggggatcatt
     6841 cacctccagg atcagtttgc cggcaatctc tcctacggcc aacagaagct cctggaactg
     6901 ggccgggcgg tgatggccga accgcagctc atcctcctgg acgaaccgac ggcggggatc
     6961 aatccccgcc tcatccggac catgatcgac atgatcctga agatgcagga ccagggacgg
     7021 accttcttca tcatcgaaca caacatggac gtggcggcgg agctttgcga acatatcttc
     7081 gtcctcgatc acggggagag aatcgcctcc gggtcgcccc gggagatcca gaacgatccc
     7141 cgggttatcg aggcctacct gggatgacgg gcatggaggg aaaagatata tatgcaggct
     7201 acgggaagat tgaagtcctc cacggggcgt cgatccgggc cggcgagggt gaaatcgtat
     7261 gcatcatcgg ccccaacggc tccggaaaat caaccctcct caaggccctg ttcggcctga
     7321 tcacaccccg gcgagggaag gtcctcttcg ccggaaggga cgtgacccac ctcgccccgg
     7381 aggaaaaggt ccggcggggg attgccttca tcccccaggg acgcaatgtc tttacctccc
     7441 tgacggtcag ggagaacctg gagatggggg gaacgccgct gggcgacgag cgggaggtgg
     7501 cccgggccat cggtgaggtc ctggaatcct atcccgtcct gaaggacaag ctgcaccacc
     7561 gggcgggaaa cctctcggga ggggaaaagc agctcctcgc cctggcccgg gccgacatga
     7621 tcaaaccccg gatgatcctc atggacgagc cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7741 nnnnnnnnnn nggatcggcg gggccttcgc cacggacagc cgggggcgga tcttcgtcgt
     7801 ccaccgcggc aaattagggg gcagtcggaa gggcgtcggc aaggccctct tcgaaaagcg
     7861 gtatcgcggg gtctggatgg aaatggatga cggcggggag gaggcatccg tggccgtggt
     7921 cggcgtcctc ccctcccccc gcttcgcccg gcaggtcgcc cagttcgtcg gcaagatcga
     7981 aaagatcaag gatatggcgg ccccctccgc ccagacggaa atcggcttcg acgacctccg
     8041 cctccgggag gaactcctgg ggacgcggcg gtgcgagctg ttccgggaca cggccgggga
     8101 gtgcgatcac ggcctggtcg ttaaggatct ggccgccgcg ctgaaaataa taggctgcaa
     8161 attcggtaat gatggtctcc gggacctcct ggccctggat ccccggggcg aggtgacgat
     8221 cttccagatc aagacggaca acctctataa gagcatccag gaaggggcca cccggctcct
     8281 tgtcaacagc ctccgcgcca cccgcccccc ccgcctggtt ctggtcctcc ccgccgcccc
     8341 cgacaccggc aaggaggaga ggctccggcg cctcaatatc gacgtcctca cctaccggtg
     8401 ggaaggaaac cacgccgttt tccccggact ggaaactttc ctggacattc cttaaccagc
     8461 ggcaggttgc ttccctcgtt cgtccgaaat agccacgaaa agcttactgc cttcccgttg
     8521 atatcatccc cggtaacgag cggaagatca ccagcaatgg gggcggcgca gggaaattgt
     8581 gcgtacggga ccgatagggt ggcacggcgg cgctggccgg ctttttgccg ctcaagggac
     8641 caggaggagc tggttgtccc gggaccggga ttcccccagg ctggttacgg tgatcgccgg
     8701 ccggcccagg accgggcatt tgttttcaca gattccacac cccacgcaga gttcaagatc
     8761 tacatggggc tgtttcaaga gcatctcccg cccccggcga tcccgcacgc gaacctcctc
     8821 gagccagatg gccttggggg aagtagggca aacctcctcg cagacgatgc aggacatgtc
     8881 atgggcaaag ggcaggcacc gccccttgtc gatcatggcc aggccgatct tcgtcgccgc
     8941 cttttcccgc aggccaagcc tgccgatggc ccccgtggga cacacctggc cgcagagggt
     9001 gcaacggtat tcgcagcagc cgatgcgggg caccaggacc ggggtccaga tcccctccag
     9061 acccgcttcc aggagggtgg gctggaggcc gcccgtaatg cagaccttca tgcattcccc
     9121 acagcggacg caacgcttaa gaaactcctc ctccgccagg gcgccggggg gacggatcag
     9181 ggtcggctcg gccgctccgg ccctggccag gggggtgatc cgcagcagcg gggccgtgag
     9241 cgccccggca acagcggcgc cgaggacccg ccttcggccc agatctaagg aaaaggacgc
     9301 cggtcgggag gaaaatccaa aagaaacggc atgttgcggg cagggatcgt cgcagttgaa
     9361 gcaatacaga cattccgcag ccacccattc cgcccccttt gctccgatcg ccccgccctg
     9421 acactcccgg cggcagcgac cacagtcgtt gcagccctcg ctaaccgatc tcttcaggag
     9481 ggcaaaacgg gacagcaggc ccaggagggc ccccaggggg cagaggtatt tgcaccagaa
     9541 gcgggactcc cggaggttga gggccaaaag ggcaaagaag agcaggccga tggcggtccc
     9601 ctggaaaaaa agaggctgcg aaaaggggag gacactcctt ttcaacagct cgtaaacgac
     9661 ctcggcgcca ctgctgacca gggggatatg ccactcatac agcatggtga agatggagcg
     9721 gagggcgtaa ctcaagaacg gataaacact cagggacagg gaacggatga ggagggtcag
     9781 gggatcgaaa agcccggccg cctggactcc tagcatggac agaacgagaa tgaagacgag
     9841 gagaagatat ttgatccggt attgtcccca gttcctgacc cggcgccggg gaacctcaat
     9901 gccggcaatg gtttcctgac gacttccggt acttcctgcg ccggtgacgg agttgtgtct
     9961 ccgggaaacg gcattccttc ttcccctcag gccttccagc aaccgaacga ccgtaatgga
    10021 acctcccccc ttcctgcggg cggcgcttct ttcccgcttc agttttcccg ccaactggtg
    10081 gagggtcccc aaagggcaga tccagccgca aaatatccgc cccaacagga tggtggcgac
    10141 gatgaccgtc agcgccaggg caaaggcgcc gtaaaaagag cgggaggcaa gggcggtcgt
    10201 tatccagagc aggggatcgg cgtcaaggaa gatcttgacg ggatagccga gttcgttggc
    10261 cccctgcgat tccgtctgga gaaagagcca gagaaagagg agaagaaaca gggcctgact
    10321 gagacggcgc agcctggtca tggtcagatg ccgacgcatt aatttaccct cctatacact
    10381 gacacgcttg atcagaagcc gggagaggtc catgttcccc aggccggcgc gatgcccgat
    10441 gcggacacaa tccagggtgt cggccttcat gccgaagagg gtggtcccga aggcatccac
    10501 ggccacctgg tccgtcccgg cgatcacggt atccaggcgc cggacatcgg caagactccc
    10561 cccctgggga ccgttgtcca caaggatgcg cacggcgtcc aggacgatca gggacgggcg
    10621 gatcacccgg gcgatgtcca cgaggctctc gtcgatgcgc tggtgaatcc gtccccgcca
    10681 gccccccatg acccccatcc agttcttcat ccctagggtc agggtggcga tgccgtgcac
    10741 cttggcgatc gggacgttga tcaccttgtc cgccgcgagg atctccgtat agagaggcca
    10801 ggacttcagc agttgacccc ggagggccgt atctttgaat ttacggtcgt ctatatagga
    10861 gacctgggcc cccgccgccg tcgccgctgc ggcaatgccg ctctgccggt aggtccgccg
    10921 ggcgtcgttg acgggaaagt cgaacacctt cacccgtttg gccccggcct cataacaaag
    10981 tcggaccagc tccgccacga cctcgggatt ggtgttggcg gcgtattccg gtgtccggtc
    11041 ccagccgata ttgggcttga cgaccacgat gtcgccccgg gagacgaatc tgcccatccc
    11101 gcccaactcc gccaccgccg cccgggtgat tcgggccggc gggacgcctc gcgccacggc
    11161 cagatcgggt cgcccggccg cctccgccag ggaacgcagg gaatcggccc ccagcggcag
    11221 ggccagggcc gccccggaca gaacagcc
//
LOCUS       DAOSMF010000062        11236 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_6650_length_11236_cov_24.011537, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000062 DAOSMF010000000
VERSION     DAOSMF010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 11236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11236
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6650_length_11236_cov_24.011537"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /gene="infC"
                     /locus_tag="PLT63_07145"
     CDS             <1..335
                     /gene="infC"
                     /locus_tag="PLT63_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOD98535.1"
                     /translation="MDYGKYRYQQSKKLQDARKSQTTIQIKEIRLRPKTEEHDLEVKI
                     RHIKKFLQQNDKVKITMMFRGREIAYSDLGRKIMETIRDALDEVGAMEMQPKLEGRNM
                     IMIVVPKK"
     gene            364..561
                     /gene="rpmI"
                     /locus_tag="PLT63_07150"
     CDS             364..561
                     /gene="rpmI"
                     /locus_tag="PLT63_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005950095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOD98536.1"
                     /translation="MPKLKTHRGAAKRFSLTGTGRVKRAKAFSNHILSTKSPKRKRNL
                     RRSAVADKTNEAALKKLLPYL"
     gene            623..982
                     /gene="rplT"
                     /locus_tag="PLT63_07155"
     CDS             623..982
                     /gene="rplT"
                     /locus_tag="PLT63_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOD98537.1"
                     /translation="MPRVKRAITAKKKRRKILKFAKGFFGARRRLLRTATEAVNRAMK
                     YAFRDRRMRKRDFRKLWITRISAAARLNEISYSRLIDGMKKAEVAIDRKILADLAVHD
                     PQAFKEIVTMAKGQIAS"
     gene            979..1995
                     /gene="pheS"
                     /locus_tag="PLT63_07160"
     CDS             979..1995
                     /gene="pheS"
                     /locus_tag="PLT63_07160"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOD98538.1"
                     /translation="MIQELKELAQRAETELQGAHTEEALNVLRTKYLGRKGLLTQMLR
                     QLGSVSTTERPLFGRLSNEIKEKISASLDAALERIKDSRRNELLCREAIDITLPGRAV
                     RIGHRHPVTQVMEEICGIFSRLGFTVEEGPEIETDYYNFEALNIPKDHPARDMQDTFY
                     VEDNIVLRTHTSPVQIRTMEKMQPPVRILSPGKVYRPDSDVSHTPMFHQIEGLLVDRG
                     ITFGDLKGTLTFFLQEVFGADILLRFRPSFFPFTEPSAEVDIRCVMCGGEGCRVCGQS
                     GWLEILGSGMVDPAVFANVGYDPEGYSGFAFGLGVERIAMLKYGISDIRLFFDNDVRF
                     LEQF"
     gene            1999..4416
                     /gene="pheT"
                     /locus_tag="PLT63_07165"
     CDS             1999..4416
                     /gene="pheT"
                     /locus_tag="PLT63_07165"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOD98539.1"
                     /translation="MKVSLKWLRDYVDVDMEPAVLAESLTMAGLEVEAVEEAGPDFDG
                     IVVARILTVRPHPQSYKLHLCEVTIGSETLPIVCGAPNTKAGTNVALARVGATIPGGY
                     TIKSSRLRGELSEGMLCSEEELGIGEDNTGIMILPPELPLGMDLKEALGLLDTVLDIG
                     ITPNRADCLSIIGIAREVAAITGNTLRYPETAVRENEEDIDGITSVTIEDPDLCPRYT
                     ARIIKNITIKPSPAWMRERLEAVGLRAINNIVDVTNFVMMELGQPLHAFDYRFLEEGR
                     IVVRRSRNGEHFVSLDEKDRVLRADTLMICDGVKPVAIAGIMGGLNSEVKDDTSTILL
                     ESAYFHPASIHRSSRWLGMGTDAAFRFERGIDPEGVVRALNRAAQLMAELGDGEICRG
                     YIDVYPRQISVSKDIPLRGRRVSEILGTNISAREAAGILERLEMTIVPAGGDDYLVTP
                     PTCRVDIAREIDLIEEIARIHGFHNIPVTMPASGVAPGRENSSRRREELLRTVMNGLG
                     YTEAINYSFINPAMAEQLGLPEGDPLRSFVKIRNPLTEEQAVMRTTLVYGLLEVMNRN
                     ARNGCADLKIFELGRVFFARGKEELPEEKACLAALVTGSRYDSSWHHHGIGADFYDLK
                     GVLESLFAVLKITGAKFIAPAAEWSFLHPGRSCRVMIGEKVAGFLGEIHPQVQERLDL
                     RSRALVLQLELDSLAVPAAAGRSFREISKYPASSRDVAFLVPAVMEGEALCRAAYNQH
                     EVLLESVSIFDVYTGKNLPEGMKSVGLRFSYRSGDRTLREEEINSAHGRIVKTMVAAT
                     GAKVRGE"
     gene            4444..4719
                     /locus_tag="PLT63_07170"
     CDS             4444..4719
                     /locus_tag="PLT63_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HOD98540.1"
                     /translation="MTKIDIIQDVYEKLGFSKKDSARIVESVFEIMKECLGRGEKIKI
                     SGFGNFVVKEKKSRRGRNPQTGKEIEISARRVLTFKSSQVLRKALNG"
     gene            4757..5098
                     /locus_tag="PLT63_07175"
     CDS             4757..5098
                     /locus_tag="PLT63_07175"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOD98541.1"
                     /translation="MDESIPDKTYFRIGEVSSILGVEPYVVRYWESEFKSVRPVRTGS
                     DQRLYRRKDLEQLLLIRNLLYRDNFTIRGAKKQLSRKNPDPSRDGLPNEDRRLLREVK
                     AELSKIRELLS"
     gene            complement(5321..5614)
                     /locus_tag="PLT63_07180"
     CDS             complement(5321..5614)
                     /locus_tag="PLT63_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PxxKW family cysteine-rich protein"
                     /protein_id="HOD98542.1"
                     /translation="MICQTIRTGADCVFMKSNGCAFKDGACKTIVEKCEGCGKIVELA
                     TGRFCQVYMDPASRWLMGNCPRATHVKLVIKENTQKINPLKASKRASRAKKKK"
     gene            5774..6520
                     /locus_tag="PLT63_07185"
     CDS             5774..6520
                     /locus_tag="PLT63_07185"
                     /inference="COORDINATES: protein motif:HMM:NF015570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HOD98543.1"
                     /translation="MKTSDLPGFFLRSLFVMSSLNFRRMQNLGFAYAILPLTRNMDEN
                     KERIVAFLQRQLEYFNTHPYLSGAVLGATIRLAEEEDAVAPTDQTATTKLKKSLMGPY
                     AAIGDNFFWGSLRPLAGIIAAILAYEGFIWAPFVFLLIFDPLHLWIRWQGFLEGYRQG
                     WRGIAYIQSWKLPLLAHYLRWVSLIILGVMTGLLTADIHLFPGQFWLRQGLNIAVLAA
                     VLLFYIAVRRGLSPLLIFYVLTAVCLVVMI"
     gene            6522..6791
                     /locus_tag="PLT63_07190"
     CDS             6522..6791
                     /locus_tag="PLT63_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOD98544.1"
                     /translation="MQEIRTFEVKNKLGIHARVAAKLVEEARRYKARIFFEYEGQEVN
                     GKSLLGILTLACGSGGRMTVKVEGSDAAKALSALERLFADKFGED"
     gene            6810..8591
                     /gene="ptsP"
                     /locus_tag="PLT63_07195"
     CDS             6810..8591
                     /gene="ptsP"
                     /locus_tag="PLT63_07195"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HOD98545.1"
                     /translation="MPVEKNKKTMVLKGIGVSPGVVTGRAYLFDRLDAQISFYKLNKA
                     GLITKEIRRFREALRASERQLKEIKSKLTEVAGLEPIYIMDVHIMILKDRSFVANVVQ
                     RIREMSVNAEWAVRLTIDKYRDIFEKMDDEYLRDRFNDIRYVGQMILRNLAGRRQETI
                     SPLGEGVIVIATDLSPADTAQMMIDKVLAFATDMGSKTSHTAIVARSIAIPAVVGLER
                     VTRQVRTNDLIIIDGTAGLVIVNPDPELLHRYEEKKHHYQTVREEALKYAKFPAVTRD
                     RCRIQIAGNIEFIEEIPSAVAHGAEGIGLYRTEFIYINREELPTEEDHFLNYRRVIGV
                     EGLEWATIRTFDLGGDKFFSDPKLAKEMNPQMGLRAIRFCLKEINLFKVQLRAILRAS
                     AFGKAKILFPMISGIEEIRAAKKILGEVRDDLKQQGISVGEDIEIGVMIEVPSAVIIA
                     EELAKEVDFFSIGTNDLIQYVLAIDRINDRVTYLYEPLHPAVLRMIRKVVEIGHRAGI
                     HVAMCGEMAGDPTYAMILLAMEIDELSMNPLAIPKVKEIIRETTLKEARQLMRRVMKM
                     ASAVEIREHVEKTMREKFSGLLAGNGS"
     gene            8610..>11236
                     /locus_tag="PLT63_07200"
     CDS             8610..>11236
                     /locus_tag="PLT63_07200"
                     /inference="COORDINATES: protein motif:HMM:NF039580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOD98546.1"
                     /translation="MTTLIGKITGWFRDSRHYRPESLYEETNFLLDWLVRKALWKISF
                     DTEVAENLRKLGNEGLVVYALKDRSQLNSLILWDLARRKGIPMPVHSHDVNMTLWQPF
                     SASLRVIASAWSRIFFRRRGEGDGNGFLAVRNHLAAGRALIIHLGGSEFFENKRVNDV
                     LNGLREAQEALDRPLYIIPVLVTYGRRREKEKESIINILFGQTDNTMFLQRLVSFLRF
                     AGKIYVMPTEPVEIARFCRENTEQLRGEAVYSLRSELLSRIDEEKSALIGPFLKSRQE
                     HIGMVLKESDLIGFMEREAAAGKKDYATVVKEAKKYLNEIAADYQEAYIEVWDRVLSW
                     LWNNIYDGVVVDLEGMARIRNVSKKMPFVIIPCHRSHIDYLLLSYVFYQHRIQLPLIA
                     AGTNMAFGPFGYLFRKSGAFFLRRSFRGNALYGEVFAKYLEVLIREGLPIEFFIEGGR
                     SRTGKMVMPKYGLLSMIIQAFQRHAAEDLAAIPVYIGYDRVIEEKSYLKELSGVPKER
                     EKTTDVIKSSRILRKRYGSVYVNIGAPIYLQAFLAAQDKSFAEMDVEERQSLYRKMGY
                     EIALAINKVSVVTPFALISAGLLSHDRRGISHDELMAILKEFYEYLIHMKVSLAATFV
                     RRDKAINDALLLFDQSGLISRLGEEEGEEPELEEIVYSLDDDKRLNLEYYKNNILHFF
                     LPISFVASSMLAYPQDHIPLGLILDDYRFLKRLFWNEFIFDTEQEDGGEVNNALAYLL
                     DREMIAIRGPEEGAEACFEVKGKGRTNLRPFAGLIENYIQSYWIVVRACAYLRKGPRS
                     EREWLKGIHKLGAIMFRKGEIRRAEALSEANYGNALIALQEEGILAVSENTEKKDRRD
                     RRIFSLTNDRRAHENLRRRL"
BASE COUNT         2719 a         2869 c         3221 g         2427 t
ORIGIN      
        1 tcatggatta cggcaagtat cgataccagc agagtaaaaa actccaggat gcgagaaaaa
       61 gccagacgac gatacagatc aaggaaatac gcctgcgacc gaagacggaa gagcatgatt
      121 tggaggtgaa gatccgtcat atcaaaaagt ttctgcaaca gaacgacaag gtgaagatta
      181 ccatgatgtt ccggggccgg gagatagctt attcggatct ggggcggaag attatggaaa
      241 cgatacgcga cgccctggac gaggtgggag caatggagat gcagccgaag ctggaaggca
      301 ggaacatgat catgatcgtg gttccaaaga aataaacatt ttatagcgag gagacgagca
      361 accatgccaa aattaaagac tcatcggggc gccgccaagc gtttttccct taccggcacg
      421 ggaagggtga agagggccaa ggcgttttcc aaccatattc tgtcgacgaa atcgccgaag
      481 cggaaaagaa atctccggag atcggcggtg gcggacaaga ccaatgaggc cgccctcaaa
      541 aaattactgc cctatctgta acttcgaggc ggtaacgatc cgaatgcatc cggttccaaa
      601 ccaatgaagg agaaaagaat aaatgcccag ggtaaaaaga gccataacgg ctaagaagaa
      661 acgtagaaag atattgaagt ttgcgaaggg gttttttggt gcgcgccggc gcctactgcg
      721 gacggcgacg gaagcagtca acagggcgat gaaatacgcc ttccgcgacc gccggatgag
      781 aaaaagagac ttccgaaaac tctggatcac caggatcagc gctgccgccc gtctgaacga
      841 gatcagctac agccgtttga tcgatggcat gaaaaaggcg gaggtagcca tcgaccgcaa
      901 gattctcgcc gatctggccg tccatgatcc gcaggccttc aaagagatcg tgaccatggc
      961 caaaggacaa atcgcttcat gatccaggaa ctaaaggaac ttgcgcagcg ggccgaaacg
     1021 gagttgcagg gagcccacac cgaggaggca ctcaatgtac tgcgaacaaa atatctgggt
     1081 cgcaagggtt tgcttactca gatgctgcgc caattgggaa gcgtctccac taccgagcgg
     1141 cctctgtttg gaagattaag caacgagatc aaggaaaaaa tctccgcctc cctggatgcc
     1201 gccctggaga gaatcaagga ttctcgccgg aatgaactgc tgtgccggga ggccatcgat
     1261 ataacccttc ccggtcgggc tgtccggatc ggtcaccggc atccggtaac ccaagttatg
     1321 gaggagatat gcgggatctt ttcccgtttg ggatttaccg tcgaggaagg gccggagatc
     1381 gagacggatt attataattt cgaggcgttg aacattccca aggatcatcc ggcccgggac
     1441 atgcaggaca ccttctatgt agaagacaac atcgtcctga ggacccacac ctcgcccgta
     1501 caaatccgga ccatggaaaa gatgcagccg ccggtgcgga tattatctcc cggcaaggtc
     1561 tatcgtcccg attccgatgt ttcccatacc ccaatgttcc atcagataga agggctcctg
     1621 gttgaccggg gcatcacctt cggggacctc aagggtaccc tgactttctt tctccaggag
     1681 gtattcggcg cggatatcct gttgagattt cggccaagtt tctttccctt caccgaaccc
     1741 agcgccgagg tggacatccg ctgcgttatg tgtgggggcg aaggttgccg ggtatgcggc
     1801 cagagcggct ggttggaaat cttgggttcc ggcatggtgg atccggcggt attcgcaaat
     1861 gtcggctatg atcctgaagg atattcaggg tttgcctttg ggctgggggt ggaacggatt
     1921 gccatgctga agtacggtat atccgatatc cgccttttct tcgataacga cgtgcgcttt
     1981 ttggagcaat tttaacctat gaaggtaagt cttaaatggc tccgggacta tgtcgatgtc
     2041 gatatggaac cggcggttct ggcggaaagc cttaccatgg cgggtctgga agtggaggca
     2101 gtcgaggagg cgggacccga tttcgacggg atcgtggtgg ccagaattct taccgtgcgc
     2161 ccccatcccc aatcgtataa gctgcacctg tgtgaagtca ccatcggctc ggagacactg
     2221 cccattgtat gcggtgcccc caacaccaag gccggcacca acgttgcctt agcccgggtc
     2281 ggcgccacaa ttccgggagg ctataccatc aagagttcac gcctccgcgg cgaactctcc
     2341 gaggggatgc tctgttcaga agaggaattg ggaattggcg aagataatac ggggatcatg
     2401 atcctccctc cggaactgcc cttgggaatg gaccttaagg aggctctggg gctgttggat
     2461 acagtcctgg atatcggcat tacgcccaat cgcgccgatt gcctgtccat catcggcatc
     2521 gcccgggagg tggcggcgat tacgggaaat accctgaggt atccggagac agccgtccgg
     2581 gaaaatgaag aggacatcga cgggatcacc tcggttacta tagaggatcc agatctttgc
     2641 ccccgttata ccgcccgaat catcaagaac atcacgataa agccttcgcc ggcgtggatg
     2701 cgggaacgcc tggaggccgt cggtctgcgg gccataaaca atatcgtgga tgtcaccaat
     2761 tttgtcatga tggagctggg ccaacccctc catgccttcg attaccggtt tcttgaggaa
     2821 ggacgcatcg tcgtccgacg ttcccgcaac ggggagcatt tcgtctccct cgacgaaaaa
     2881 gaccgggtgc tgagggccga tactctgatg atttgtgatg gtgtcaaacc cgtggccatt
     2941 gcgggcatca tgggcggatt gaattcggag gttaaagacg acacctccac cattcttctg
     3001 gagagcgcct atttccatcc ggcctccatc caccgttctt cccgctggct gggcatgggt
     3061 accgatgcag ccttccgctt cgaacgaggc attgaccccg aaggggtggt gcgggcattg
     3121 aaccgggcgg cacaacttat ggcggaactt ggagatggag aaatctgcag gggatacatc
     3181 gatgtttacc cgcggcaaat cagtgtctca aaagacatac ccctgcgggg gcggcgggtg
     3241 agcgagatat tgggaacgaa catttccgcc cgtgaggcag ccggaattct ggagcgattg
     3301 gagatgacca ttgttcccgc gggtggtgac gactatctgg ttacccctcc cacctgccgg
     3361 gtggatatcg cccgggagat cgatctgatc gaagagattg cccggatcca tggtttccat
     3421 aatattcccg tcaccatgcc cgcatcaggg gtcgctccgg gacgggagaa ttccagccgt
     3481 cggcgcgagg agcttctccg cacggtgatg aacggactgg gctatacgga ggcgatcaat
     3541 tacagcttca tcaacccggc gatggcggaa caactcggtc tgccggaagg agatccactg
     3601 cgttcgtttg taaagatccg caatcccctg acggaagaac aggccgtcat gaggaccacc
     3661 ctggtctatg gtctgctgga ggtcatgaac cggaatgccc ggaacggatg tgcagacctg
     3721 aaaatattcg aactcggccg ggttttcttt gcccggggca aggaggaatt gccggaagaa
     3781 aaggcttgcc tggccgccct ggtcaccggc agccgttatg attcctcctg gcaccaccat
     3841 gggatcgggg cggattttta cgatctgaag ggcgtgctgg aatcactttt tgctgtcctg
     3901 aaaataaccg gggcaaaatt tattgcgccg gcggctgaat ggtcttttct tcatcccgga
     3961 cggtcctgcc gcgtcatgat cggggaaaag gtcgcgggtt tcctcgggga gattcatccc
     4021 caggtgcagg aaaggctgga tttgcgcagt cgcgccctgg tgttacaact ggagctggac
     4081 agccttgcag ttcctgccgc ggcgggaaga tcattccggg aaatatcgaa gtatcccgcc
     4141 agttcccggg atgtggcgtt tctagtaccg gcggttatgg aaggagaggc actgtgccgg
     4201 gctgcttata accaacatga agtattactt gaaagtgtct cgatttttga tgtatatacg
     4261 ggcaaaaatc ttccggaagg aatgaagagc gtcggtctgc ggttttcgta tcgatccggt
     4321 gacaggacct tacgggagga agaaatcaac agtgcccatg gccggatcgt aaaaaccatg
     4381 gttgcggcga caggggcaaa ggtccgtggt gaataaaacg gcgagatgac aggaggagaa
     4441 acaatgacga agattgatat tattcaggac gtatacgaga aactcggctt ttccaagaag
     4501 gattctgcac ggattgtcga atcggtattt gaaatcatga aagaatgcct gggtcgggga
     4561 gaaaagataa agatatcggg attcggcaac ttcgtcgtca aggagaagaa gtcgcggcgg
     4621 ggccgtaatc cacagacggg caaagaaata gagatttcgg cccgtcgcgt ccttaccttc
     4681 aagtcgagcc aagtcctgcg aaaggcgctc aacggctgat ccgggatcgt aaaccaacaa
     4741 tgacgggagt ctgattatgg atgaatcgat tcctgacaag acttacttcc gcattggaga
     4801 agtgagcagc atcctcggtg tggaacctta tgtggtccgc tactgggagt cggagttcaa
     4861 atcggtacga cctgtccgca ccgggtccga tcagcggctt taccgccgta aggatctcga
     4921 gcaactgcta ctcatcagga acctcctgta tagagacaac tttactattc ggggtgcaaa
     4981 aaaacagctt tccagaaaaa atccggatcc gagccgtgac gggctgccaa atgaggatcg
     5041 acggctcctc agagaagtaa aagcggaatt gtctaaaatc agggagcttc tttcctgaga
     5101 ccgccagagc cgctcgggga agatccgcca aggctaatac gacagccatc ttccctccac
     5161 cggcggtgat ccaaacggat agatcatttc cgccttcccc ccggtgttcc tttaaattcc
     5221 tgatccttgc cgacatctca gccgaggccc gccggtattg acggtctctt tttacaaatc
     5281 gccggagttg tcttccatcc ctgcgggacg cgagggcagg ttatttcttt ttcttcgcgc
     5341 gactcgcccg cttggaggcc ttcaaggggt tgatcttctg ggtgttttcc ttgattacca
     5401 gcttaacgtg ggtggcccgg gggcaattgc ccataagcca tctgctggcc ggatccatgt
     5461 atacctggca gaagcgaccg gtcgccagct cgactatctt gccgcagcct tcgcattttt
     5521 ccacaatcgt tttacaagcc ccatccttga aggcgcagcc attgctcttc atgaacacac
     5581 aatcggcgcc cgttctgatc gtctgacaaa tcatcttttt cacctcaaaa aaaagttggt
     5641 atttctaaca aataaccatg gttctgtcaa ggataaaggg gtcaaaacat ggggaatgaa
     5701 ttgacttaaa aggtccgtcg tgctagaaga accgctatct actgccatgg cggctgggga
     5761 gaaaaggttg aggatgaaga catcggatct gcccggtttt tttttgcggt ccctcttcgt
     5821 gatgtcgtcc ctgaatttca ggagaatgca gaatctgggt tttgcctatg ccattttacc
     5881 cctgacccgc aacatggatg aaaacaagga aaggatcgtg gcctttcttc agcgccagct
     5941 cgaatacttc aacacccatc cctatctatc cggcgccgtc cttggcgcaa cgataagact
     6001 ggcggaagaa gaagacgccg ttgcccccac cgatcaaacc gcgacaacga aactgaaaaa
     6061 atcccttatg ggtccctatg ccgccatcgg cgacaatttc ttctggggga gtctccgacc
     6121 cctggccgga atcatcgcgg cgattctcgc ttatgagggg tttatctggg cgccgttcgt
     6181 ttttcttctg atcttcgatc ccctgcattt atggatccgt tggcaaggat ttcttgaagg
     6241 ctaccgccaa ggatggcggg ggatcgctta tatccagtcc tggaaactgc cgctgctggc
     6301 tcattacctg cgctgggttt ccctgatcat ccttggggtg atgaccgggt tgcttaccgc
     6361 ggacatacac ctttttccgg gacagttttg gctgcggcag ggactgaata ttgccgtgct
     6421 ggcagcggta ctgctgtttt atattgccgt gcgccgggga ttgtcgccgc tactgatctt
     6481 ttacgtgctg accgccgttt gtttggtggt gatgatttga tatgcaggag ataaggacct
     6541 tcgaggtaaa gaacaagctg ggaatccatg cccgggtggc ggcaaagctg gtagaggagg
     6601 ccagaagata caaggcgaga atattctttg aatatgaagg acaagaggtc aacggcaaga
     6661 gcctcctcgg cattctgact ttggcgtgtg gatcgggagg cagaatgacg gtcaaggtag
     6721 agggcagcga tgccgccaag gcgctgtctg ctctggagcg actgtttgcc gataaattcg
     6781 gagaggatta gcaaaaggta acaatagtta tgcctgtaga aaagaacaaa aaaactatgg
     6841 tgctcaaagg aatcggtgta tcacctgggg tggtgacggg acgggcttac cttttcgatc
     6901 gcctcgatgc ccagatttcc ttttacaaac tcaacaaggc aggcctgata acaaaggaaa
     6961 tcaggcgttt ccgggaagca ttgcgggcct cggagcggca gttgaaggaa atcaagagta
     7021 aactgacaga ggtagcgggc ctggaaccga tctatattat ggatgtccat attatgatcc
     7081 tcaaagacag atcatttgtc gccaacgtgg tccagcgaat tcgtgaaatg tctgtaaacg
     7141 cggaatgggc cgttcgtttg accattgata aataccgcga catatttgag aaaatggacg
     7201 atgaatacct gcgggaccgc ttcaacgata tccgttatgt cgggcagatg atcctccgca
     7261 atcttgccgg acggcgccag gaaacgatct cccccctagg tgaaggggtg atcgttatcg
     7321 ccacggattt gtcacctgca gataccgccc agatgatgat cgacaaggtg ctggcttttg
     7381 ctaccgacat gggaagtaag acctcccata cggccattgt ggcccgatcc atagccatcc
     7441 cggcggttgt gggactggag agggtaacca gacaggtccg gacgaacgat ctcatcatca
     7501 tcgacggcac cgccggtctg gtcatcgtca atcccgatcc ggagctcctg caccgctacg
     7561 aggagaaaaa gcaccattac cagacggttc gggaagaagc gcttaaatat gcaaaatttc
     7621 cggctgttac cagagaccgt tgccggatcc agatcgccgg caatatagag ttcatcgaag
     7681 aaattccctc cgccgtcgcc cacggagccg agggaatcgg actgtatcgt accgagttca
     7741 tatacatcaa ccgggaagag ctgccgacgg aggaggacca cttcctcaat tatcgccggg
     7801 tcatcggtgt agaggggctg gaatgggcaa ccatccgcac cttcgatctg ggtggcgaca
     7861 agttcttttc cgacccgaaa ctggccaagg aaatgaatcc ccagatgggg ctccgggcca
     7921 ttcgtttctg cctaaaggaa atcaatctgt tcaaggtgca gctccgggcg atcctccggg
     7981 ccagcgcctt cgggaaggca aagatcctct tccccatgat ttcgggcatc gaagaaatac
     8041 gtgccgcgaa gaagatcctc ggagaggtcc gtgatgatct gaaacaacaa ggaatctcgg
     8101 tgggcgagga tatcgagatc ggtgtcatga tcgaggtgcc atcggcggtc atcatcgccg
     8161 aggaactggc caaggaagtc gattttttca gtatcggcac caacgatctg attcagtacg
     8221 tcctggccat cgaccggatc aacgaccggg ttacctatct ctatgaaccc ctccaccccg
     8281 ccgtcctcag gatgatcaga aaagtggtgg aaataggcca ccgggccggc attcatgtgg
     8341 ccatgtgtgg ggaaatggcc ggagacccca catatgcgat gatcctgctg gccatggaaa
     8401 tcgacgaact gagcatgaat cccctggcca ttcccaaggt aaaggaaatc atcagggaaa
     8461 cgaccctgaa agaagcgcga cagctcatga gaagggtaat gaaaatggcc tcggcggtgg
     8521 aaattcggga acatgtggaa aagaccatgc gggaaaaatt ttccggtctt ttggccggta
     8581 acggatctta ggagcacagg tggccatcca tgacgacttt gataggcaag ataaccggct
     8641 ggttccggga cagccgccat taccggccgg aaagtctgta cgaagaaacc aatttccttt
     8701 tggactggct ggtccggaag gccctgtgga agatctcttt tgatacggag gttgccgaga
     8761 atctgagaaa actcggcaac gaaggtctgg tggtttatgc cctgaaggac cggagccagc
     8821 tcaacagcct catcctctgg gatctggccc ggcggaaggg gatacccatg ccggtacaca
     8881 gccatgacgt aaacatgacg ctatggcagc ccttttccgc ctccctgcgc gttatcgcct
     8941 cggcttggag tcgtattttc ttccgccgtc ggggtgaggg agacggcaac ggcttcctgg
     9001 ccgtaaggaa tcatctggct gctggtcggg ccctgatcat tcatttaggg ggatcggaat
     9061 tcttcgagaa taaaagagtc aacgatgtcc tgaacggtct ccgggaggca caggaagctc
     9121 tcgatcggcc gctgtatatc ataccagtgc tggttaccta cggacggaga cgggagaaag
     9181 aaaaggagag catcatcaac atcctcttcg gccagacgga caacacaatg ttcctccagc
     9241 gactggtctc gtttctccgc tttgccggca agatatacgt catgcccacg gagccggtgg
     9301 agatagcaag attttgccgg gaaaacaccg agcagctccg gggggaagcg gtctatagtc
     9361 tgcgcagtga actgctcagc cgcatcgatg aggagaagtc ggcgctgatt ggaccttttc
     9421 tcaagtcccg ccaggagcat atcggcatgg tgctaaagga atcggatctc atcggcttca
     9481 tggaaagaga ggccgctgct ggcaagaagg actatgccac cgtcgtgaag gaagccaaaa
     9541 aatacctcaa cgagatcgcc gcggattacc aggaggcgta tatcgaggtg tgggaccggg
     9601 tactttcatg gctatggaac aacatctatg acggcgtggt tgtggacctg gagggcatgg
     9661 ccaggatccg caatgtttcg aaaaagatgc ccttcgtcat cattccctgc caccgaagtc
     9721 acatcgatta tttactttta tcttatgtct tttaccagca tcgcatccaa ctgcccttaa
     9781 ttgccgccgg caccaatatg gccttcggcc ctttcggtta tctcttccgc aagtccgggg
     9841 cgttctttct taggcggagc tttcgtggca acgccctgta cggggaggtc ttcgcaaaat
     9901 atctggaggt gctcatccgg gaaggactgc ccattgaatt tttcatcgag ggagggcgga
     9961 gccgcaccgg caagatggtg atgccgaaat acgggctgct gtccatgatc attcaggcct
    10021 tccagaggca tgcggcggag gatctggcag ccattcccgt ctatattggc tatgaccggg
    10081 tgatcgaaga gaaatcatac ctcaaggagc tgagcggggt gcccaaggag cgggaaaaga
    10141 ccacggatgt catcaagagt tcacgaatcc ttcgtaaacg gtatggcagc gtctatgtga
    10201 atatcggcgc gccaatatat ctgcaggctt tccttgcggc tcaggacaag agctttgccg
    10261 agatggacgt ggaggagcgt cagagcctct atcggaagat ggggtatgaa atcgccctgg
    10321 ccataaacaa ggtttccgtg gtcacgccct tcgctctgat ctcggcgggg ctactgtccc
    10381 atgaccgccg cgggatcagc cacgacgaat tgatggcgat cctcaaggag ttttacgaat
    10441 atctgatcca tatgaaggtc agcctagcgg ccacctttgt ccgccgcgac aaggccatca
    10501 acgacgccct cctgttgttc gatcaatcag gactgatttc gcgacttggg gaagaggagg
    10561 gagaggaacc ggaactggaa gaaattgttt acagcctcga cgacgacaaa cgtctcaacc
    10621 tggaatacta caaaaacaac atcttgcatt tcttcctccc catcagcttc gtcgccagct
    10681 ccatgctggc ttatccccag gaccatatcc ccctgggact gatcctggat gattacagat
    10741 tcttaaaacg tttgttctgg aacgagttta tcttcgacac ggagcaggag gacggcggcg
    10801 aggtcaacaa cgccctggcg tatctcctgg atcgggaaat gatcgcaatc cggggaccgg
    10861 aagagggcgc tgaggcctgc ttcgaggtga aggggaaagg gcggaccaac ctccgtccct
    10921 ttgcgggact gatcgaaaat tacatccagt cctactggat cgtcgtccgg gcgtgcgcct
    10981 acctccgaaa aggaccccgc tccgagcggg agtggctgaa aggcatccac aaactggggg
    11041 cgattatgtt ccgtaagggt gagatccgcc gggcggaagc gctatccgag gccaactacg
    11101 ggaatgcctt gatcgccctg caggaggagg ggattcttgc agtttcggaa aacacggaga
    11161 agaaagaccg tagggacaga agaatattct ccctgacaaa cgaccggcgg gcccacgaaa
    11221 acttgcgccg gcgtct
//
LOCUS       DAOSMF010000063        11218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_6661_length_11218_cov_17.900385, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000063 DAOSMF010000000
VERSION     DAOSMF010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 11218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11218
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6661_length_11218_cov_17.900385"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2048)
                     /locus_tag="PLT63_07205"
     CDS             complement(<1..2048)
                     /locus_tag="PLT63_07205"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOD98547.1"
                     /translation="MRNFLEPGSVAIIGAPRKTGSDTYNGVEMMLRYGYQGRIFPINP
                     KADKICGLKVYPYITDVPETADLAVISLGRDHLLPAFKDCIQAGIRSVIIISQGFADA
                     DGIGKKLQAEIVKLARESGTRIIGPNTMGVLNNFQNFTTAFIDLNKPDKVPPVSLVAQ
                     TGLIQVGSQDFSYHAWGKALDLGNASDVDFVDALEYFETDPQTQVIVLHMEGIIRGRD
                     FLKAASRISVNKPIIIYKTGRTKGGARAAISHTASLVGEDEINDAAFRRAGIIRVKNA
                     SELRDAIRSLVKLEDMAGPRLGILTPTGAGGIMAVDACEDFGLTIGKLPEGLASKLKQ
                     GIPDWVHIGNPVDIWPVGMLGGSYSQAFKIALMELLKSPSIDGVLAMTVNVHSHLHKG
                     IDVVEAVRAARIEAGNRKPIAMWIYGEHTGSILERYESIDGVACFDSIDQAMQGLSFC
                     YRYHQIRNRSLSVQRRFPYKRELAESLLQKGRERKVLLGEDALALLSVFGIPAIQGKL
                     AGTWKEIQAAADMLSYPLVLKLSGDAFLHKSEWGGVVTGIHNKGELHRAFQDMKKNVR
                     ERNPMLKIGFQVQEQAKGKELLLGLKRDHQFGHILACGFGGIYTEIFKDISRELVPVS
                     RREAEKMLSSLKIHPLLKGVRGEAGVDWEGLLDIVERLSFLAAEVPDIAELDINPLMA
                     "
     gene            complement(2077..2439)
                     /locus_tag="PLT63_07210"
     CDS             complement(2077..2439)
                     /locus_tag="PLT63_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98548.1"
                     /translation="MRQNDLVNPRRGYYLIILFAAIFNTSSRLAAGYLFDKCRRMSIL
                     LMVFLLQAFNLMLFVNYVTPILLASGTVFTGLCYGAFFALFPLATAVFFGVTNFGINY
                     GLIFLAWGFVGMILNNDS"
     gene            complement(2655..3527)
                     /locus_tag="PLT63_07215"
     CDS             complement(2655..3527)
                     /locus_tag="PLT63_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOD98549.1"
                     /translation="MKYEGMIIRPPSEARSLLLQVTVGCSHNKCTFCRAYKAKKFRIK
                     SFAEIEEDILEASRYISIEKVFLVDGDALIIPQKRLLEIMLSIRQHLKSVKKIGTYGN
                     AKSILRKTPEELAQLRELGMQIVYLGVETGNDDLLKRINKGATYAQLVEAGRRIKDAG
                     IKLSVTVLLGIGGIEKSSEHARDTAKILTDIDPDYVGALTVIVAPGTPLYEEYISGRF
                     VLPDPFGYLKELEIMVADSDFTNCLFTSNHASNYLPINVSLPDEKEKTVRMIQQVIQS
                     GDNRRLRPEYIRGL"
     gene            3814..5598
                     /locus_tag="PLT63_07220"
     CDS             3814..5598
                     /locus_tag="PLT63_07220"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOD98550.1"
                     /translation="MSIFLGTGMAEPRTLVNHLMASDQSNLQDLEIIQLISLGDTVSV
                     DDRYSKKYRLKTFHSGWIASASIREGRVDLIPSRFSRISRLFKSGAIKIDMAFIQITP
                     FNESGYASLGVGVDVTRHAMEHARVVVGEINEQIPRTLGDTLVHVEEFDFFVMATEPP
                     IYLERWPVDQVYNKLAAYVTSVVPDGSCLSFSIGPLYDALALHLIHKKNLGIHTPFFT
                     DALMDIIESGAVTNRCKTFFPGKSLASTALGTKELMRWLDRNPLVEFQPIDVTQEPKN
                     IGANSNFIAILPARKADLTGDIGLHAGRGNVIAGRGAVQELFAGAALSTNGRTLFALP
                     SRNRKGESNIVLSIVDYPYRFSNRDSLDMIVTEYGVAYLTGRTIRERAQALIDIAHPD
                     DRMNLIQQAKEAKFLYADQIYLPESGHLYPEDIACTHTFKNGLVVRIRAIKPSDEDEM
                     RRLFYRFSEQAIYYRYFNPIKTMPHDRMQEYVNIDYRTVISIVVVIEEAEREKIIAEA
                     RYIRLKDMDQSYADIAFIVDEDYHGLGIASFLLTSLIRIAKERGVRGFTADVLIDNAP
                     MLRVFEKAHFSVKGLPRQGICSLTFTFE"
     gene            complement(5730..6623)
                     /locus_tag="PLT63_07225"
     CDS             complement(5730..6623)
                     /locus_tag="PLT63_07225"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOD98551.1"
                     /translation="MIIESKGQIHDNLYAIGSSEFPGYLLKGKRPVLFDAGMTFMGPL
                     YLVELENLLGDINSLAFHFLTHSHFDHAGASPFLKRSIPGLKIGASPAAAETFRKPNA
                     IELIKNLSRSLEEKNSALIGGADVTFNGLEIDLVLSDGMEIELADGLVCRVIATPGHT
                     RDSLSFYIPKMKAIITGEAIGAFDRNFTIHPEFTSSYQDYMASLEKLAALDIEIILMC
                     HYFTLTGADARGYMQKAIMGAQAFKERIEHYLMNANGDRQAVVKKIFQEDYEATGAIL
                     QDSRPYLINLDAKVRVVAEVR"
     gene            6949..7917
                     /locus_tag="PLT63_07230"
     CDS             6949..7917
                     /locus_tag="PLT63_07230"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HOD98552.1"
                     /translation="MMFQTDITRMFGIKYPIVVGTMAHLSCAEMVAAASNAGALGVLS
                     SATFLTGEAFRNEVKKVKDLTDRPFAVNLNLFPSMKPIDNMEYLEIIADEGIKIIETS
                     GNKAPEEIAKKAKEHGILMIHKCVGVRYALKAQSIGAAVVTVVGYENGGATGTLDITT
                     LCLVPRVVDALNIPVIGGGGVADGRGFLALLALGAHGVIMGTPFLVAEECPIHRNLKE
                     ALIRATELDTMIVMRGIKNSHRVWTNEASKKVAELERQQAGIEDIIHAASGERVQKMY
                     RNGDVNAGIISCGQGVGLIKKIRPLKEIIEEIVFQADELRRNLVAS"
     gene            complement(7969..9105)
                     /locus_tag="PLT63_07235"
     CDS             complement(7969..9105)
                     /locus_tag="PLT63_07235"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOD98553.1"
                     /translation="MILFLLTFFFLYGGLHLYFFLRLRAAFSLPVGLQITVGPLLLLL
                     LVSPLLVRMAERHGYEAATCLLAYTGYLWMGFIFLFFVVSVCLDLYRLAVFLNERIGH
                     QDLSHLFPPARIIFLGPLVIAIILNVYGYYEAMSVRTEHLVIKTAKLPAQVEKFRIVQ
                     ISDLHIGMIVRDGRLKRFLAAVEAAAPDVLVSTGDLVDGQLDNLYEALARLKNVPVKY
                     GKFAVTGNHEFFAGVTRAVEFTEKAGFTVLRGEGVNVAGIVNIAGVDDPAVTRRGEGK
                     QVREAEFLSSLPPGVFTVLLKHQPVADPDAEEKFDLQLSGHTHRGQIFPFNMVTKFVY
                     YYHSGDFYLPDGARLHVSRGTGTWGPPVRLLAPPEITVIDLVRK"
     gene            9280..10911
                     /locus_tag="PLT63_07240"
     CDS             9280..10911
                     /locus_tag="PLT63_07240"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HOD98554.1"
                     /translation="MIYITIMAAPEFDLLIINGCVYDGLGHLGVEVDLAVKRDRIALL
                     SKGIATARAGTVIDARGMAVAPGFIDPHSHTDIELLVNPRAESKIRQGVTTEIAGNCG
                     YTVFPLSQLNYEERRRYCAEQYDVPLIWRDLEGFFSELESKGIAVNYATFMGHGTLRS
                     AVFGTQDRPPTPAEMSAMKALIRENMQMGALGLSTGLIYVPGCFADRQELISLCRETA
                     VYGGIYSTHMRNESDYLVEAVEEAITIAQEAHISLQISHLKLAYPRNWSKIHWVLSRI
                     SEVNAQGLEILADRYPYTASSTVLSSFMPPWIQQGTTSEYLARLTDPANEEKIRNYVQ
                     IQEEKVGSWDNVFISSVLRKDNEHLAGKSIRRAARDAGKEPYIFIRDLLISEEDQVWM
                     INFSLNEDNFKRIILHPLVIIGSDGWARAPYGPLGKDKPHPRSYGTFPRMLGRYVREE
                     GIMSMERAIEKMTSLTARKFGLYGRGIIKEGNFADLVVFAPEQIIDRSTWTDPHQYPE
                     GIPYVIVNGIPVIYEGEHTDAFPGRILRKSQTVVL"
     gene            complement(11031..>11218)
                     /locus_tag="PLT63_07245"
     CDS             complement(11031..>11218)
                     /locus_tag="PLT63_07245"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOD98555.1"
                     /translation="VTEAINAEEEAFGERRLIETVSSQRHRSAREIISRVEEEVMSFA
                     GGQPQFDDITMMVLKVL"
BASE COUNT         2720 a         3003 c         2841 g         2654 t
ORIGIN      
        1 tcggccatca ggggattgat gtccagctcc gcgatgtccg gtacctcagc agccaggaaa
       61 gacaaccttt ccacaatatc caaaagcccc tcccagtcaa caccggcttc accgcggact
      121 cctttcaaga gcggatggat tttcagagac gaaagcatct tttcggcctc ccttctgctg
      181 acgggcacga gttcgcggct gatgtccttg aagatttccg tgtatatccc tccaaaaccg
      241 caggcgagga tatggccaaa ctgatggtca cgtttaagac ccagaagtag ttccttccct
      301 ttggcctgtt cctgtacctg gaagccgatt tttaacatgg gattacgttc gcgtacattt
      361 ttcttcatgt cctgaaaggc ccgatgaagt tcccctttgt tgtgaattcc tgtcaccaca
      421 cccccccact cactcttgtg aagaaaggca tcaccggata acttaaggac caagggatag
      481 gaaagcatgt cagccgctgc ttgaatttcc ttccatgttc cggcgagctt cccttgaatc
      541 gcggggatgc cgaagacgga aagaagggcc agggcatcct ctcctagcag aaccttgcgc
      601 tccctacctt tctgaaggag tgattcggcc agctcacgtt tataagggaa tctccgctgg
      661 acagagaggc tccggttcct gatttgatgg tagcggtaac aaaacgataa tccctgcatg
      721 gcctggtcaa tggaatcaaa acaggcgaca ccgtctatgc tctcataccg ttccaagata
      781 gagccggtat gttcgccata tatccacata gcgataggct tccggttccc tgcctcgatc
      841 cgtgcagctc gcaccgcctc aacgacgtcg atacccttgt ggagatgtga gtgcacatta
      901 actgtcattg ccaagacgcc atcgatactc ggtgatttca acagttccat cagggctatt
      961 ttgaaagcct gggaataact gcctcccagc ataccaacgg gccagatgtc gacgggattg
     1021 ccgatatgca cccagtcagg tatcccctgc ttgagtttac tggcaagccc ttccggcaat
     1081 ttcccgatcg taagcccaaa atcctcacag gcgtccaccg ccatgatgcc tcctgcaccg
     1141 gtgggggtca ggattcccag ccgaggcccc gccatatctt caagcttcac cagggaccgg
     1201 atggcgtctc tgagttcgga agcattcttt acgcgaataa tccccgcacg gcgaaaagcg
     1261 gcgtcattta tctcatcctc tccgacaaga gaagccgtat gggagatagc cgccctggcc
     1321 cctcccttgg tccggccggt tttataaatg attatcggct tgttcacaga tatccgggaa
     1381 gccgccttca gaaaatcacg gccccgaatg ataccttcca tgtgcaggac tatcacctgg
     1441 gtctgtggat cagtctcgaa atattccagg gcatcgacga aatccacatc gctggcattc
     1501 ccgaggtcaa gagcctttcc ccaggcatgg taggagaaat cctgtgagcc cacctggata
     1561 agtcccgtct gggcgacaag ggataccggc ggaaccttgt ccggcttgtt cagatcgata
     1621 aaagcggtgg taaaattttg gaaattgttt aaaactccca tcgtgttggg accaataatc
     1681 cttgtaccac tctcgcgggc aagcttaaca atctcggcct gcaacttctt gccaatcccg
     1741 tcggcatctg caaaaccctg actgatgatg atcaccgaac ggatgccggc ctgaatacag
     1801 tctttaaagg caggaagcag gtggtccctg ccaagcgaga ttaccgccag atcagccgtc
     1861 tccggcacat ctgttatgta gggatacacc ttgaggccac agattttatc ggctttcggg
     1921 ttgatcggaa atatgcgtcc ctgatacccg tagcggagca tcatctcgac cccattatag
     1981 gtatccgatc ccgtttttct tggtgcaccg atgatggcca cagatcccgg ttctaaaaaa
     2041 ttcctcattt taagacctcc ataattaaaa aattatttat gagtcattat tcaagatcat
     2101 tccgacaaat ccccaggcaa gaaatatcag accataattg attccgaaat ttgtcacgcc
     2161 gaaaaaaacc gctgtggcga gtgggaacaa agcaaagaag gcgccataac agaggcctgt
     2221 aaatacagtc cccgaggcca gcaatatggg agtgacgtaa ttgacaaaca gcatcagatt
     2281 aaaagcttgg agtagaaata ccatcaaaag aatactcatg cgacggcatt tatcaaataa
     2341 ataacctgct gccagtctgc tgctcgtgtt gaatatggcg gcgaataaaa tgatcaggta
     2401 atatcctcgc ctcgggttta ccaggtcatt ctgtcgcatt tttgtggagg gtccttgaac
     2461 tcaatcggat tcctttactg taagatcaaa atgagtatca aaattttcca gctactgatg
     2521 gatttgtgca cggcatactg cttcagatta tacttaaaca ttaaagtgtg tcaagcagaa
     2581 ttagttgtcg ctttatctgc cttgccctgg aaccgtcccg tttacgggac gatcccgact
     2641 ctcaagattc ttctttataa tccccgaata tattccgggc gtaatcgacg gttgtcgcca
     2701 gattggatga cttgttgaat catccggacg gttttttcct tttcatccgg caaactgacg
     2761 ttaatgggga ggtaattcga tgcatgattg gacgtgaaca ggcagttcgt aaaatcacta
     2821 tcggcaacca taatttccag ttccttcaga tatccgaaag gatccggaag cacaaaccgg
     2881 ccactgatat actcttcgta tagaggagtc ccgggagcga cgataaccgt cagggcgccg
     2941 acataatcgg ggtcgatatc cgtaaggatt ttcgctgtat cgcgagcatg ttcgctgctt
     3001 ttttcgatcc cgccgatgcc gagcagcacc gtaacagaga gtttgatccc ggcatccttg
     3061 atcctccggc ccgcttcaac cagttgggcg taggtcgcgc ccttgttgat cctcttgagg
     3121 aggtcatcat tcccggtttc cacacccaaa tagacaatct gcataccaag ttcacgcaat
     3181 tgcgctagct cctcgggcgt cttgcgcaga atgctcttgg cgttgccgta ggtccctatc
     3241 tttttaacgc tcttaaggtg ctgcctgatg gataacataa tctccagcag tcgcttctgg
     3301 gggatgatca acgcatcacc atccacgaga aaaactttct ctatggagat atagcggctg
     3361 gcttccagga tgtcttcctc aatctccgca aaggatttga tgcgaaattt tttcgcttta
     3421 taggcacgac aaaaggtgca tttgttgtgg gagcagccaa cggttacctg caaaagcagg
     3481 ctacgggcct cgctgggtgg tctaataatc attccttcat atttcaattt attgtattac
     3541 cccttaaaga ccgctgccag aggccgcgga tacttgttcc tttctgtttg attgtctatt
     3601 gcgttggcta tttttaatcc attatgacga aaaaggcaag ggtgacgacg gacctgatta
     3661 taattgacac acaagtactt ttttcttata cttcctcaaa atggaagttt tacattcatt
     3721 gggggattga ttcccatgac cgatagaaga agcgtttcct ggagggaaaa ggtcgtatca
     3781 cccggagacg tcctgaaacg gattgaaccg gggatgagca tctttctggg aacaggcatg
     3841 gctgaacccc gtacgctggt caatcacctc atggcatccg atcaatcgaa tctccaggat
     3901 ctggaaataa ttcagttaat cagccttggg gatacggtgt ccgtggatga ccgctattcc
     3961 aagaaatacc ggctaaaaac ctttcactcc ggctggattg ccagcgcctc catcagagag
     4021 ggtcgggttg atctgatccc aagccggttt tcccgcattt ccaggttgtt taaatcgggg
     4081 gcgatcaaga tcgatatggc cttcattcag attaccccct ttaacgaatc cggctatgcc
     4141 agtctcggtg tgggggttga tgtgacgcgg catgccatgg aacatgcaag ggtagtggtg
     4201 ggcgagatca acgagcaaat tcccagaacc ctgggcgata ccctggtgca cgtcgaggaa
     4261 ttcgactttt ttgtcatggc aacagaaccg cctatttacc tggagcggtg gcctgtcgat
     4321 caggtttata ataaacttgc ggcgtatgtt acctctgtcg taccggatgg aagctgtctc
     4381 tcattttcca tcggtccgct ttatgatgcc ttagcactgc atctaatcca caaaaaaaac
     4441 ctgggaattc atacgccgtt ttttaccgat gccctgatgg acatcatcga aagcggcgca
     4501 gtgacgaacc gttgtaagac attcttccca ggaaaatctc tggcctcaac ggccttgggt
     4561 accaaggaac tcatgcggtg gctggatcgt aacccgctgg tggaattcca acctattgat
     4621 gtcacacagg agccgaagaa catcggagcg aatagcaact ttatcgcgat tctgccggcg
     4681 agaaaagcgg atctgacggg ggatatcggt ctgcacgcgg gccggggaaa tgtgatcgcc
     4741 ggtcgcggtg ccgtgcagga gctttttgcc ggtgcggcgc tttcaacaaa tggtcggacg
     4801 ctctttgccc tacccagccg aaatcgtaaa ggagaatcaa atatcgtcct atccatcgtg
     4861 gattatccct accggttttc caaccgggac tctctggata tgatcgtaac ggagtatggc
     4921 gtggcttatc tgacgggacg gacgatccgg gagcgtgcgc aagcattgat cgacatcgct
     4981 catccggatg acagaatgaa tctgattcag caggcaaagg aagccaaatt cctctatgcc
     5041 gatcaaatat atcttcccga gtcggggcat ctatatccgg aagatatcgc ctgcactcac
     5101 accttcaaga atggtttggt cgtccgtatc cgggcgatca aaccatcgga cgaagatgaa
     5161 atgcgtcgtt tattctaccg gttctccgag caggcgattt attaccgcta cttcaatccc
     5221 atcaaaacca tgccgcacga ccgcatgcag gaatatgtca atattgatta cagaacggtc
     5281 atatcgattg ttgtagtgat agaggaagcg gaacgggaaa agatcatcgc cgaagcccgt
     5341 tatattcgtt tgaaggatat ggaccaatca tacgccgata tagcctttat cgtggatgaa
     5401 gactatcatg gcctgggcat cgcatctttc ctgctcacct cgctcattcg tattgccaag
     5461 gaacgcggcg ttagaggttt tactgccgat gtactgatcg acaatgcgcc catgctcaga
     5521 gttttcgaaa aagcccactt ctctgtcaaa ggcctgcccc gccaggggat ttgctccctg
     5581 acttttacct ttgaataaaa caacccctat tcacacatcc ttcaaatgat gttcattgaa
     5641 taattcaatt tcaggaccgg gattttcttt ccgaatttga tgttatcatg gattgtcccg
     5701 gaggagaact atatcatggg gagttcgaat tacctgacct cagcgaccac ccgcaccttg
     5761 gcatccaggt tgatcaggta cggccgggaa tcctgaagaa tcgctccggt agcttcgtaa
     5821 tcctcctgga aaatcttttt cacgaccgcc tggcgatcgc cgttggcatt catcaagtaa
     5881 tgttcaattc tttccttaaa agcctgggcg cccataatgg ctttctgcat gtatccccgg
     5941 gcgtctgcgc cggtcagtgt aaaatagtgg cacatcagga tgatttcgat gtccagtgcg
     6001 gccagttttt ctaaggaagc catatagtcc tgatagctgg atgtgaattc cggatgaatg
     6061 gtaaaattcc tgtcaaaggc ccctatcgcc tcgccggtaa taatggcttt cattttaggg
     6121 atatagaagg atagggaatc cctggtatga ccgggggttg cgataacgcg gcagacaaga
     6181 ccgtcagcca gttcgatctc cataccgtct gaaaggacaa ggtcgatttc gagaccgtta
     6241 aaggtcacgt ccgcaccgcc tatcagggcg ctgttttttt cttccagtga gcggctgaga
     6301 ttcttgatga gttcaatggc gttaggcttg cggaaagtct cggcggccgc tggactggcc
     6361 ccaatcttaa gcccgggaat gcttcgtttc agaaagggtg acgctccggc atgatcgaag
     6421 tgagaatggg tcaagaaatg aaaagccaag ctattgatgt ctcccagaag gttttccagc
     6481 tcaacaagat aaagaggccc cataaacgtc atcccggcgt cgaatagcac cggccgttta
     6541 cccttcagta gatacccggg aaattcggaa gaccctattg catatagatt gtcgtggatt
     6601 tggcccttgc tttcaataat cactcgtctt tctccttata gtcgttatgg ccatttcatg
     6661 gccgacttgg caactcctca attcaacaag aacttgcctt tgtctttttt gatcatatgt
     6721 aataatagcc tgagccgtca attaaattat gggataaata ggatttctcc atctgtgcgt
     6781 ctttgtctct gattataggg gaaaatacat ggcattgact acctcaacta caaaaaactc
     6841 tatgaaccct gccagaactg cgatataggt ggcgggcagt tacattatat tttcaaaatg
     6901 tgggtcatca atgactatcg tttccattga tgcccaggga tcgggggaat gatgtttcag
     6961 accgatatta ccaggatgtt cggtatcaaa tacccaattg tggtggggac catggcgcac
     7021 ctgtcctgcg ctgagatggt agcggcagct tccaatgccg gggcgctcgg tgtcttatcc
     7081 tcggctacat tcttgaccgg agaagcgttc cgcaatgaag tcaagaaagt caaagacctt
     7141 accgacaggc cttttgccgt caatttgaat ttattcccgt ccatgaaacc catcgataac
     7201 atggagtatc tggaaatcat cgctgatgag ggaatcaaaa ttattgaaac ttccggcaat
     7261 aaagcacctg aagaaatcgc gaaaaaggcg aaagagcatg gaattctgat gatccataaa
     7321 tgtgtcggag tgcgctatgc cttgaaggcg cagtcaatag gagcggcggt ggtcactgtt
     7381 gtcgggtacg aaaatggagg ggccacgggt actctggaca tcaccacgct ttgcctcgtt
     7441 ccccgagtcg tcgatgccct gaacatcccg gtaatcgggg gaggaggcgt tgcggacggg
     7501 cgcggttttc tggcgctact ggcgctgggg gctcatggtg tcatcatggg aacccctttc
     7561 ttggtggccg aagagtgccc aattcaccga aatttaaaag aggcgctgat ccgggccacc
     7621 gaactggata cgatgatcgt aatgcgcggc atcaaaaatt ctcatcgcgt ctggactaat
     7681 gaagcgtcaa agaaagttgc ggagctggaa aggcagcagg ccggaataga ggacattatt
     7741 catgcggctt ccggagagag ggttcagaaa atgtaccgga acggagacgt gaatgccggc
     7801 attatctctt gcgggcaagg agtgggattg attaagaaaa taaggcccct gaaggagatt
     7861 atcgaagaga ttgtatttca agccgacgaa ttgcgccgca atctggtggc ttcctgaagt
     7921 ttggcgtaat attctccgtt gctttaatac attacatccc caccttggtc atttccgcac
     7981 cagatcgatg acggtaattt ccggaggggc aaggagacgc accggtggtc cccaggtccc
     8041 ggtgccccgg ctcacatgga ggcgggcgcc gtcgggcagg tagaaatcgc cgctgtgata
     8101 gtaatagaca aactttgtca ccatattgaa gggaaagatc tggccgcgat gggtatggcc
     8161 ggacaactgg agatcgaatt tttcctccgc gtccggatcg gcgacgggct ggtgcttaag
     8221 caggacggta aagactccgg gcggcagcga ggaaaggaat tccgcttccc gcacttgctt
     8281 gccttcccct ctcctcgtga ccgccggatc gtcaacgccg gcaatgttga ctattcccgc
     8341 cacattgacc ccctcacctc tcagcacggt aaagccggct ttttcagtga attcgacggc
     8401 ccgggtaacg ccggcgaaga actcatgatt tcccgttacg gcaaatttcc cgtatttgac
     8461 cgggacattt ttcagccggg ccagggcttc ataaagattg tcgagctggc cgtcaacaag
     8521 atcaccggtg gataccagca catccggcgc cgccgcctcc accgccgcca gaaacctttt
     8581 caaacggccg tcgcgaacaa tcattccgat atggagatcg gagatctgga cgatccggaa
     8641 tttctccacc tgggcgggga gtttcgccgt tttgatcact aaatgttccg tgcggacgct
     8701 catcgcttcg tagtatccgt aaacattgag gataattgcg atgacgaggg ggccaaggaa
     8761 aattatccgc gccgggggaa acagatggga cagatcctga tggccgatcc gctcattgag
     8821 gaaaaccgcc aggcggtaca agtccagaca gacagaaacg acaaaaaaga gaaatataaa
     8881 ccccatccag agatagcctg tataagccag gaggcaggtg gcggcttcat aaccgtgacg
     8941 ctccgccatg cggacgagca gggggctaac gagcagcagg agcagcaacg ggccaacggt
     9001 gatctgcaat cctacgggaa gagaaaaggc cgcccggaga cggaggaaga aatacagatg
     9061 cagcccgccg tatagaaaga aaaatgtgag gagaaaaagg atcattatgt acaagggtac
     9121 ctagtactta gcaaacatgg ggttcatcgt cgtccctgcc cccacgccga tgctcctgcc
     9181 gtccttcacc cagaccgcgg cgaccgtcgc cgaaatcgtt ttatccttca tgtttttaaa
     9241 tccgcctttc cgatcggccc ttgccgcgat gatgcattaa tgatatatat caccattatg
     9301 gcggcgccgg aattcgatct actgattatc aacggctgtg tctatgacgg cctcggtcat
     9361 ctgggtgtgg aagtggacct tgccgtgaag agggatcgca tcgccctgct gtccaaaggt
     9421 atcgcaaccg ctcgtgccgg caccgtcatc gatgcccggg gcatggccgt ggcccccggc
     9481 tttatcgatc ctcacagcca caccgacatc gaactcctcg tcaatccccg ggcggaaagc
     9541 aagatccgcc aaggcgtcac cacggaaatc gccggcaact gcggctatac cgtctttccc
     9601 ctctcccaac tcaactatga ggaacggcgg cgctattgcg ccgagcaata cgacgttccc
     9661 ctcatctggc gcgatctgga gggcttcttc agcgagttgg aaagcaaggg catcgctgtc
     9721 aactacgcca ctttcatggg tcacggcacg ctgcggagcg ctgtcttcgg cactcaggac
     9781 cggccgccga cgcccgcgga aatgtcggcc atgaaggcgc tgatccggga gaacatgcag
     9841 atgggcgccc tgggactttc cacggggctc atctatgtgc cgggatgttt cgccgaccgc
     9901 caggagctca tctcgctctg ccgggaaacg gcggtttacg ggggcatcta cagcacccac
     9961 atgcgcaacg aatcggatta cctcgtcgag gcggtggaag aggccatcac catcgcccag
    10021 gaggcgcata tcagtcttca gatctcccac ctgaaacttg cctatccccg gaactggtcg
    10081 aaaatacact gggtcctctc ccggatatcc gaagtgaacg cccaggggct ggagattctc
    10141 gccgaccgct atccctatac cgccagctcc accgtcctgt cgtccttcat gccgccgtgg
    10201 atccagcagg gcaccacctc ggagtacctg gcccgcctca cggacccggc caatgaagag
    10261 aaaatcagga actacgtcca gattcaggag gaaaaggtcg gttcctggga caatgtcttc
    10321 atctcctcgg tcctccgcaa ggacaacgaa cacctggccg gaaagagcat ccgccgcgcc
    10381 gcccgggacg ccggcaagga accctacatc tttatccggg acctcctcat ctccgaggaa
    10441 gaccaggtct ggatgatcaa cttttccctc aatgaagaca acttcaaaag gattatcctc
    10501 catcccctgg tgatcatcgg ctccgacggc tgggctcggg ctccctacgg tcccctggga
    10561 aaagacaaac cccacccccg ctcatacggg actttccctc ggatgctggg aagatacgtc
    10621 agagaagagg gaattatgag catggaaagg gccatcgaaa agatgacttc gctgacggcc
    10681 cggaaattcg gtctctatgg ccgtggaatc attaaagaag gcaattttgc cgacctggtg
    10741 gtctttgctc ccgagcagat aatcgacaga tccacctgga cggaccccca tcaatacccc
    10801 gagggcatcc cctacgtcat cgtcaacggc atcccggtca tctacgaggg agaacataca
    10861 gacgccttcc ccggccgcat cctccgaaaa agccagaccg tcgtcctgta gaaccaccgg
    10921 ccggagatcg catccttgct gccatctgcc ggttacaccc cgcctatgcc ttgttgagtc
    10981 cgtggaatgg ggcggcccgc gatctgcgtc ctgtcgcaat tgtttaccta ttacagaacc
    11041 ttcagcacca tcatggttat gtcgtcgaat tgcggctgcc caccggcaaa gctcatcacc
    11101 tcttcctcta cccggctgat gatctcccgg gcggaccggt gccgctgcga actgacggtc
    11161 tcaatgagcc gccgctcgcc gaaggcctcc tcttcggcgt tgatcgcctc cgtcaccc
//
LOCUS       DAOSMF010000064        11121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_6727_length_11121_cov_19.933942, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000064 DAOSMF010000000
VERSION     DAOSMF010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 11121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11121
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6727_length_11121_cov_19.933942"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /locus_tag="PLT63_07250"
     CDS             <1..775
                     /locus_tag="PLT63_07250"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98556.1"
                     /translation="SQDPSPQKEIDKQGLQELIGAYTAAAGRAWKAGFDGIQIHMAHG
                     YLLSQWLSPAFNNRTDAYGGDIENRCRLHREICGAIRETVGRDFPLLIKINCEDYIEN
                     GLTRKDALAVVKSLTAGGVDAVELSGGTIASGRLSPSRSRINAPEKEAYFREAARIFK
                     ENQDVPLLLVGGIRSREVAAGLLAKGAADFISLSRPLIREPGLIRRWEDGDHRPAACI
                     SDNLCFAPAFEGRGIYCVTAEREAARCGKIVDETAVEIP"
     gene            812..1054
                     /locus_tag="PLT63_07255"
     CDS             812..1054
                     /locus_tag="PLT63_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98557.1"
                     /translation="MGVTDRNSHVQINVLLSLAIFMTRLDAYIVAIAIPPIAENFYAT
                     SSEAAPVILSYMLFMTSSLLILGKYSDRTDMKKVLI"
     gene            complement(1180..1836)
                     /locus_tag="PLT63_07260"
     CDS             complement(1180..1836)
                     /locus_tag="PLT63_07260"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOD98558.1"
                     /translation="MAQKELQSPAFFAASRKEVLLFTLLVGAAVAAAYFYLDLPLARA
                     GNELPFQVGVFFSYVTVLGESTWSLIAAALLGLAARFLWRRDDWMRRSLFVFTAVAAS
                     GLITDLIKWLAGRWRPKAYFTDHFYGFDLFGVGYEQTSFPSGHATTIWACGVALAILF
                     PRWRLLWYALAILVSMSRVIIGAHYLSDVLAGAYVAVMTVLVLVRLRYFRLGRPDAGA
                     "
     gene            complement(1841..3508)
                     /locus_tag="PLT63_07265"
     CDS             complement(1841..3508)
                     /locus_tag="PLT63_07265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOD98559.1"
                     /translation="MKVNWDRLTLLFLGGMTLLRLLVAPTFGLGVDEAHYFLYAYFLD
                     WSYVDHPPLVGWVHWPLYKLFGTNEFLVRLPAIILFAAVSLFLHRWVLSFSNSPLVAF
                     LSVVAVNSSFLFNALSVMLLPDCFLLLIIFPLMTAMGNIAAAPRGQARRRDFVMLGLL
                     LGLAGLAKYTAVLFLPPLLVYLLLKKRYDLLYAPGMYLAAGIALVVISPVLVWNIQND
                     FISFHYQGGHVLAATAPSAKSFLVSLLAQFGAYSPPLFLVAFYGLYRSGRETDDRIRL
                     AVLFGVTILLFFFYSSLYERTLPHWPSLFYLLFIPVGAYSLLGSSSRVQRNLFFFSVG
                     FSLTLTLFLAAALPAKWFTFPDYRSPFRDIVGYELIAREGDALLRRGPAGAEREDRRD
                     KASGELSPGRADGAERSGRGIVPVPPVAPAPEYGRPARNGGQGPIAEASPTGESGKAV
                     VAPAPRRAVAVTNWTMGSRMIYYSLPYGREVFVLDNRPDQFDVWQPAAPLGYDLLFVK
                     THFHGLDICPAYRCTACEVAGKLDIVLNGGKVDSVEFVWCRNYQGIR"
     gene            complement(3819..5837)
                     /locus_tag="PLT63_07270"
     CDS             complement(3819..5837)
                     /locus_tag="PLT63_07270"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOD98560.1"
                     /translation="MESPLFKDIIVIFLVSVTVIYLFSKLRIPSIVGFIVAGILAGPA
                     GLKLVGSEHNVKVLAEFGVVALLFTIGLEFSLRTLLKMKGAVLIGGGVQVGLSILAGM
                     AFAMMGGYPAGQAVFFGCLLSLSSTAIVLKLLQDQSQIDSPQGRAALAILIFQDIVAI
                     PMILLTPLLGGAGGDVGGSLLTLLLKGVLTIVAVYIGARWVIPYILFNVAKTKNRELF
                     LLVIIVICLAVAWMTNAIGLSLAFGAFLAGLIISESEYSHDAIGHILPFRDVFLSFFF
                     VSIGMLLDIEVVFSEPVIVCLLILGVILLKTAAAFLSVLFEGLPLRAALIAGLSLSQI
                     GEFSFVLSLVGVKYGLMGGQVYQVFLAVSVITMVLSPFLLRIAPAVADQVVRWPLPDR
                     WKTGSPIHNHVTRTRRRDHLIVIGYGLNGRNVSRAAAKAAIPYMILEMNPETVRRERK
                     AGEPIYYGDATHEAVLRHADIEYARAMVIVINDPPATRRITELAKQMNSKLHVIVRTR
                     FIQEMKVLYDLGADEVIPEEFETSVEIFSRVLRKYLIPKPEIEQFIAEVRAAGYDMLR
                     NVAPESITCSDLKICFPDAEIATFRIGGDAPAAGRSVAELALRKRHGVTLLTIRRGEG
                     MIYNPEAETLILAGDILVVFGAPEKLAAAADFFSPPVAEPAMETVEGR"
     gene            5980..6696
                     /locus_tag="PLT63_07275"
     CDS             5980..6696
                     /locus_tag="PLT63_07275"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOD98561.1"
                     /translation="MAVLEWTKDENVAVITMTNGENRHNPTFTEAILKAFDEIEANGD
                     ITAVVITSNDPKNWSQGIDLLWMMDVFGKKDFQAIKDFMYGLNRIFKRILLYPMPVVA
                     AINGHAFGDGSIMACACDFRFMRSDKGFFCFPEVDISIPFLPGMFALLRKAMPYYKFE
                     EAVFSGRRMGAKELEEHHVIVKACADNEELMKESVAFAKAFKKKRAIFGEMKRRMHKH
                     IVELMDKEDPEYIEPLKLMM"
     gene            6712..7857
                     /gene="lptF"
                     /locus_tag="PLT63_07280"
     CDS             6712..7857
                     /gene="lptF"
                     /locus_tag="PLT63_07280"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HOD98562.1"
                     /translation="MNKIIDRYILKEIAFPFFMILFILTFVLLMGKILQLMDLMVNKG
                     IRVTDIALLVIYLMPSFLLFTIPISLFVAIMMGLGRLSGDNELLVLKAAGLSLHRLAR
                     PILVAVAACFLLTAVTSYYLAPHGNFAVKTLLFDLARQKASVGIKEKVFNDEFSGILL
                     YADRVPVSGEHMEGVLISDTRNTAEQRTIIAERAYLVADPRAMTVTLRLENGATHSVD
                     RNLNNYRRMDFGRYDLKLDFGTAMAGDAEKTKASTEMTVGEMVRRMRDKKLKDEDLRE
                     LAIEINKKFSIPFSTLVFGILGIPLGIRKHRTVKYWGFTIGLAVVITYYLLRLGGEAL
                     AETGRLTPFIGAWAPNIILGVAGIWLFMRAAAEKPLWTGFRGRRASP"
     gene            7854..8936
                     /gene="lptG"
                     /locus_tag="PLT63_07285"
     CDS             7854..8936
                     /gene="lptG"
                     /locus_tag="PLT63_07285"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HOD98563.1"
                     /translation="MRILDRYLIREYLKLFLLLMVSIISIYLIIDFFGKIRMFISNNA
                     TFTQMAAHFFYMIPTIVSQTTPAVILLATLMTFGAMSKHNEIVAVKANGVSLYRLSLP
                     LVFICLAVSIVLFFFSELITPRSFHRAEYIRLVEVQKQEALGIFKQNEIWYRGSNTIY
                     NVRMFDVNNNSLHGITIHYLDPSFRLVKRIDAGKARWRDGKWVFENVLISSQSPAGGF
                     PKLERHDIQEIDLPEKPDDFKQVQKEADKMGFFELYPYIRKLEREGYDATRYRVDLHG
                     KPAFSVVSLILLAIGISFSLRSERSGGVAQSIGAGVVIGFSYWLVFAFSLSLGRSGNL
                     PPILAAWLPNLLFGGAAYYLIRRVNT"
     gene            complement(9078..10502)
                     /gene="ahcY"
                     /locus_tag="PLT63_07290"
     CDS             complement(9078..10502)
                     /gene="ahcY"
                     /locus_tag="PLT63_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOD98564.1"
                     /translation="MAYKKIDPKLAYKVADINLAAWGRKEMKLAENEMPGLMAVRKKY
                     GPQKPLQGKKVMGSLHMTIQTAMLIETLKELGADLRWASCNIFSTQDHAAAAIAAAGS
                     AAVFAWKGESLEDYWWCTEQAMTWPDGSGPDLLVDDGGDATLMVHQGVKVEKHPEILN
                     EKQENKEFAIIMERLARGYKRDPQRWQRIAAGIIGVSEETTTGVHRLYQMQQRGELLF
                     PAINVNDSVTKSKFDNLYGCRESLADGIKRATDIMVAGKVVVICGYGDVGKGSAQSMR
                     GFGARVIVTEVDPICALQAAMEGYEVQTMEEVVATADIFVTATGCCDVITGAHMERMK
                     DEAIICNIGHFDSEIAMNYLEGNPRCRKETIKPQVDKWTLASGRSIIVLAEGRLVNLG
                     CATGHPSFVMSNSFTNQCLAQMELAKKRYRPGVYTLPKLLDEEVARLHLDRLGVKLTK
                     LTKAQADYLGVPTRGPFKPDHYRY"
     gene            complement(10637..>11121)
                     /locus_tag="PLT63_07295"
     CDS             complement(10637..>11121)
                     /locus_tag="PLT63_07295"
                     /inference="COORDINATES: protein motif:HMM:NF014792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HOD98565.1"
                     /translation="PTGEWQAVNSCSAADSGVTGRKLVVQLYGGYPGAQIGGGSIINK
                     TPEKVDCSATFGARYVAKNIVAAGLAKRCSVQIAYAIGIARPISIYVNTFGGGQLEDD
                     KLGKIVSEIFDLTPRGMIEAFDLLNSRTYKKIARTLFLDDYPWEKTDRVEALRQAAGR
                     "
BASE COUNT         2290 a         3489 c         3331 g         2011 t
ORIGIN      
        1 gagccaggat ccatcccccc agaaggaaat agataaacag ggtttgcagg aactcattgg
       61 cgcttatacc gcggcggcgg ggcgggcctg gaaagctggc ttcgatggca tccagatcca
      121 catggctcac gggtatctcc tgagccagtg gctctccccg gccttcaaca accggaccga
      181 cgcctacggc ggggacatcg aaaaccgctg ccggctccac cgggagatct gcggggcgat
      241 ccgggaaacc gtgggccggg acttccccct cctgatcaag atcaattgtg aagactatat
      301 tgaaaacgga ctgacccgaa aggatgccct ggcggtggta aaatccttga cagcgggggg
      361 ggtggacgcc gtcgaactga gcggagggac catcgccagc ggccgcctct cccccagccg
      421 gtcgcggatc aatgccccgg agaaagaggc ctacttccgg gaggcggcca gaatcttcaa
      481 ggaaaaccag gacgtgccgc tccttttggt gggcgggatc cggtcccggg aggtcgccgc
      541 cggcctcctg gccaaaggcg ctgccgactt catctccctg agccgccccc tcatccggga
      601 accgggcctg atccgccgct gggaagacgg agaccaccgt cccgccgcct gcatctccga
      661 caatctctgc tttgcccccg ccttcgaggg ccgggggatc tactgtgtca ccgcggagcg
      721 ggaagcggct cgatgtggaa agatcgtcga tgaaacggcg gtggagatac catagacaaa
      781 aaccgatcca taaactcacc gtcaacaggc aatgggcgtt acggaccgga actctcatgt
      841 tcagatcaac gtgcttttga gcctggccat ctttatgacc aggctcgacg cctacatcgt
      901 ggccatcgcc atacctccca tcgcggaaaa cttttacgcc acctccagcg aagcggcgcc
      961 ggtcatcctc tcatacatgc tttttatgac cagttccctg ctgatcctcg gcaagtactc
     1021 cgaccggacg gacatgaaga aggtcctgat ctaaggcttt ccgcggctta ttgccggcga
     1081 cggtcatgat caggagacgg ttgggccgcg atccgggaca gccttcaagg tggttgtcga
     1141 gtaggaccgc ctccggcggg gcggcggtca cggatcccct caggccccgg catcggggcg
     1201 ccccagtcgg aaataccgca ggcgcaccag cacgagcacc gtcatgacgg ccacgtaggc
     1261 cccggccagg acgtcgctga gataatgggc gccgatgatc acccggctca tgctgaccag
     1321 gatcgccagg gcataccaga ggaggcgcca gcggggaaaa aggatcgcca gggccacccc
     1381 gcaggcccag atggtggtgg catggcccga ggggaaggag gtctgctcgt agcccacgcc
     1441 gaagagatcg aatccgtaaa aatgatcggt gaaataggcc ttgggacgcc accggcccgc
     1501 gagccatttg atgagatccg taatgaggcc cgaggcggcg acggcggtga aaacgaagag
     1561 ggaacgacgc atccagtcgt cgcgccgcca gaggaagcgg gccgccagac ccaggagggc
     1621 ggcggcgatc agggaccagg tcgattctcc caacacggtg acgtacgaaa aaaaaacgcc
     1681 cacctggaaa ggcagctcat tgccggcccg ggcgaggggc agatcgaggt agaaataggc
     1741 cgccgcgacg gccgcgccga ccaggagggt aaagagcagg acctccttcc gggaagcggc
     1801 gaagaacgcc ggggactgca attccttttg cgccattcga tcacctgatc ccctggtagt
     1861 tgcggcacca gacgaattcc acggaatcca ccttgccgcc gttgagcacg atatccagct
     1921 tccccgccac ttcgcaggcg gtgcagcgat aggccggaca gatgtccagc ccgtgaaaat
     1981 gagtcttcac gaagaggaga tcatacccca gcggcgccgc cggttgccac acgtcgaatt
     2041 gatccggacg gttgtccagg acgaagacct cccggccgta gggaaggctg taatagatca
     2101 tccggctccc catcgtccag ttggtcaccg ccacggctcg ccggggggcg ggggcaacga
     2161 cggccttccc gctctcgccc gtcggggatg cttcggcgat gggcccttgc ccgccattcc
     2221 gtgccggccg cccgtattcc ggcgccgggg cgacgggcgg aaccgggacg atccccctgc
     2281 cgctccgctc cgcaccatcc gcgcgtcccg gggaaagctc cccggaggct ttgtcccgcc
     2341 gatcttcccg ttccgctccc gctggtcccc ggcgcagcag ggcgtccccc tcccgggcga
     2401 tcaactcgta gcccacaatg tcccggaagg gcgaccggta atccgggaag gtgaaccatt
     2461 tagccggcag ggccgcggcg agaaaaagcg tcagcgtcag gctgaagccg acggaaaaaa
     2521 agaagagatt cctctgtacc cggctgctgg aaccaaggag agaataggcg cctacgggga
     2581 tgaacaggag atagaacagg ctcggccagt gggggagggt ccgctcgtac agggaagagt
     2641 aaaagaagaa caacaggatc gtcacgccga aaaggacggc gaggcggatg cggtcgtcgg
     2701 tctcccgccc gctccgatag aggccgtaaa aggccactag aaacagcggg gggctgtagg
     2761 ccccgaactg ggccaggaga gagacgagga acgacttggc gctcggggcc gtcgccgcca
     2821 ggacatgccc cccctggtaa tggaaactaa tgaaatcatt ctggatattc cagactaaaa
     2881 cgggactgat cacgacgagg gcgatccccg ccgccaggta catccccggg gcgtacagga
     2941 ggtcgtagcg tttcttcagg aggagataga cgagcagggg cggcaggaag aggacggcgg
     3001 tgtatttggc caggccggcc aggccgagga ggagccccag catgacgaaa tcccgcctcc
     3061 gcgcttgtcc ccgcggagcc gcggcgatgt tccccatcgc cgtcatgagg gggaagatga
     3121 tcaggaggag gaagcagtcg gggagaagca tgacgctcag ggcattgaag aggaaggagc
     3181 tgttcacggc cacgacggac aggaaggcga ccagcgggga gttggaaaag gaaaggaccc
     3241 accggtggag gaacagcgag acggcggcaa agaggatgat cgccggcagc cgcaccagga
     3301 attcattcgt tccgaacagt ttgtaaaggg gccagtggac ccagcccacc aggggggggt
     3361 gatccacgta actccagtcc aggaaatagg cgtagagaaa ataatgggcc tcatcgacac
     3421 ccaggccgaa ggtgggggcc accagcaggc gcaggagggt catgccgccc agaaagagca
     3481 gggtcaggcg gtcccagttc accttcacgg gcggaaacct cttgacgcac ggggccggcg
     3541 cccacgctcc tcactcctca tccccggctc gcttagcgct gcccgtttgt cggccatcgt
     3601 taacccctga cccggacaag ccggaaccgc acaggaatcc aaatttccgt caaaaaatgc
     3661 ccggacctta tcgctgggcg gctgacgatg aactcccttc ctatcacatc catttatcga
     3721 ccatttcgct ctcttttcgg tggaattgca tcggccatgg aaacaacggt tgagttcctt
     3781 tgataattgc gccgaaacga tcccccgtcc tgcatgcctc atcggccctc caccgtttcc
     3841 atcgccggct ccgcgaccgg gggggaaaag aaatccgcgg cggcggccag tttttccggg
     3901 gcgccgaaga ccaccaggat atcccccgcc aggatcagcg tttccgcttc cgggttgtag
     3961 atcatcccct ccccccggcg gatggtcagg agggtcaccc cgtgacgctt ccgcagggcc
     4021 agctccgcca cgctccggcc cgctgcgggg gcgtccccgc cgatccggaa ggtggcgatc
     4081 tccgcgtcgg gaaagcagat cttcaagtcg gagcaggtaa tggattccgg cgcgacgttg
     4141 cgcaacatgt cataacccgc cgccctcacc tcggcgatga actgctcgat ctccggcttg
     4201 gggatcaggt atttccgcaa cacgcggctg aagatctcca cggaggtctc gaattcctcg
     4261 gggatcacct cgtcggcccc cagatcgtac aggaccttca tctcctggat gaagcgggtc
     4321 cggacgatca cgtggagttt ggaattcatc tgtttggcca gttcggtgat ccgccgcgtc
     4381 gccgggggat cgttgatgac gatgaccatc gcccgggcgt actcgatgtc ggcgtggcgc
     4441 agtaccgcct cgtgggtggc gtcgccgtag tagatcggct ccccggcttt ccgctcccgc
     4501 cgcaccgtct cgggattcat ctccaggatc atgtagggga tcgccgcctt ggccgcggcc
     4561 cggctcacgt tccggccgtt gaggccgtaa ccgatgacga tcaggtgatc ccggcgccgg
     4621 gtccgcgtca cgtggttatg gattggtgag ccggtcttcc agcggtccgg caggggccag
     4681 cgcaccacct gatcggccac ggccggggcg atccgcagga gaaaggggct caacaccatg
     4741 gtgatcaccg atacggccag aaagacttga tagacctgac ctcccatgag tccgtacttc
     4801 acccctacca gggagaggac aaaggagaat tcgccgatct gactgaggga caacccggcg
     4861 atgagggccg cccgcagcgg cagtccttcg aagagcaccg acaggaaagc cgccgccgtc
     4921 ttcagcagga tcacccccag gatcagcagg cagacgatga ccggctcgga aaacaccact
     4981 tcgatatcga ggagcatgcc gatggatacg aagaaaaagc ttaaaaatac gtcccggaag
     5041 gggaggatat ggccgatggc gtcatggctg tactccgatt ccgagatgat cagcccggcc
     5101 aaaaaggccc cgaaggccag ggacaggccg atggcattgg tcatccaggc gacggccaga
     5161 cagatcacga tgatcaccag gagaaacagc tcccggttct tcgtcttggc gacattgaac
     5221 aggatatagg ggatgaccca gcgggcgccg atgtaaacgg cgacgatcgt caagaccccc
     5281 ttgagcagca gcgtgaggag cgatccgccg acgtcgcctc ccgccccgcc caacagaggg
     5341 gtcaggagga tcatggggat ggcgacgatg tcctgaaaga tgaggatggc cagggccgcc
     5401 cgtccctgcg ggctgtcgat ctgggactga tcctgaagga gcttcagcac gatggccgtg
     5461 ctgctcaggg agaggaggca gccgaaaaac actgcctgcc cggcaggata cccccccatc
     5521 attgcgaagg ccatgccggc caggatggaa agacccactt gcacgccccc gccgatgaga
     5581 accgcccctt tcatcttgag gagcgtccgg agggaaaact ccagaccgat ggtaaacagc
     5641 agcgccacga cgccgaattc cgccaggacc ttcacgttgt gctcggaacc caccagtttc
     5701 agtccggcgg gaccggccag gatgccggcg acgatgaagc cgacgatgga ggggatgcgg
     5761 agcttggaaa agagatagat gaccgtcacc gagacgagga aaatgacgat aatgtctttg
     5821 aacagcggac tttccatgtc tccttgtacc cttcggccgg gggaaatgtc aaatggatta
     5881 tcgacgctaa gattattgaa atttcacggc tattgtgcta actcacagca gcgtcgccaa
     5941 tagtcgccgc ctaaattacc cctagggagg aacatagata tggccgtatt ggaatggaca
     6001 aaagatgaga acgtcgccgt cattaccatg accaatggcg aaaaccgcca caaccccacc
     6061 tttaccgagg ccattttaaa ggcctttgac gaaatcgaag ccaacggcga catcaccgcc
     6121 gtcgtcatca cctccaacga ccccaagaac tggtcccagg gcatcgacct gttgtggatg
     6181 atggacgtct tcggaaagaa ggacttccag gccatcaagg acttcatgta cggcctcaac
     6241 cggatcttca agcggatcct tctctacccc atgcccgtcg tcgccgccat caacggccat
     6301 gccttcgggg acggcagcat catggcctgc gcctgcgact tccggttcat gagatccgac
     6361 aagggattct tctgcttccc cgaggtggac atcagcatcc ccttcctccc cggcatgttc
     6421 gccctgctga gaaaggccat gccctactac aaattcgagg aagccgtctt cagcggcagg
     6481 cgcatggggg ccaaggaact ggaggagcat cacgtcatcg tcaaggcctg cgcagataat
     6541 gaagaactca tgaaggagtc cgtggccttc gccaaggcct tcaaaaagaa gcgggccatc
     6601 ttcggggaga tgaaaagaag gatgcacaag cacatcgtgg agctcatgga caaggaagac
     6661 ccggaataca tcgaacccct gaagctcatg atgtagatct ccatgaggat catgaacaag
     6721 atcatcgatc gctatatcct gaaggagatc gccttcccct ttttcatgat cctcttcatc
     6781 ctcaccttcg tcctgctcat ggggaagatc ctccaactca tggacctcat ggtgaacaag
     6841 gggatccggg tcacggacat cgccctgctg gtcatctacc tgatgccgtc gtttcttttg
     6901 ttcaccatcc ccatctccct gttcgtggcc atcatgatgg ggctgggccg tctctccggc
     6961 gacaacgaac tcctggtcct gaaggcggcg ggcctgagcc tccaccggct ggcccgcccc
     7021 atcctcgtgg ccgtggcggc ctgcttcctg ctcacggccg tgaccagcta ctatctcgcc
     7081 ccccacggca atttcgccgt caagaccctc ctgttcgacc tggcccggca gaaggccagc
     7141 gtcgggatca aggaaaaggt cttcaacgac gaattcagcg gcattctcct gtacgccgac
     7201 cgggtccccg tgagcggtga gcacatggag ggggtcctga tctccgatac ccggaacact
     7261 gccgaacagc gcaccatcat cgcggagagg gcctatctcg tcgccgatcc ccgggccatg
     7321 accgtaacgc tgcgcctgga aaacggcgcc acccacagcg tggaccggaa tctcaacaac
     7381 taccggcgaa tggacttcgg ccgctatgac ctcaagctgg atttcggaac ggcgatggct
     7441 ggcgacgccg agaagaccaa ggccagcacc gaaatgaccg tgggagaaat ggtgcggcgg
     7501 atgcgggaca aaaagctcaa ggacgaggat ctgcgggagt tggccatcga aatcaacaag
     7561 aagttctcca tccccttctc caccctggtg ttcggcatcc tcggcatccc cctggggatc
     7621 agaaaacacc gcaccgttaa atattgggga ttcaccatcg gcctggccgt ggtgatcacc
     7681 tattatctcc tgcgcctggg cggcgaggcc ctggcggaaa cggggcggct gacgccgttc
     7741 atcggcgcct gggcccccaa catcatcctg ggggtcgccg ggatctggct gtttatgcgg
     7801 gcggcggcgg aaaaacccct ttggaccgga ttccggggaa ggcgtgccag cccatgagaa
     7861 tactggaccg ctatctcatc cgggaatatc tgaagctctt tttgctgctg atggtttcca
     7921 ttatttccat ctatctgatc atcgacttct tcggcaagat ccggatgttc atcagcaaca
     7981 acgccacgtt cacccagatg gcggcccatt tcttctacat gatccccacg atcgtttccc
     8041 agaccacacc cgccgtgatc ctcctggcga ccctcatgac cttcggcgcc atgtcgaagc
     8101 acaacgagat cgtagccgtg aaggcaaacg gcgtcagcct ctaccgcctc agcctcccgc
     8161 tggttttcat ctgcctggcg gtgagcatcg tcctcttctt cttcagcgaa ctcatcaccc
     8221 cccgctcctt tcaccgggcg gaatacattc gcctggtgga ggtccagaag caggaggccc
     8281 tggggatctt caagcagaac gagatctggt accggggaag caacaccatc tacaacgtcc
     8341 ggatgttcga cgtgaataac aactccctcc acggcatcac catccactac ctcgatccct
     8401 ccttccggct ggtaaagcgc atcgacgcgg gaaaggcccg ctggcgggac ggcaaatggg
     8461 tcttcgagaa tgtcctgatc agttcccaat cccccgccgg cggattccca aagctggaga
     8521 ggcatgacat ccaggagatc gatcttccgg aaaaacctga tgatttcaaa caggtgcaaa
     8581 aagaggcgga caagatgggc ttcttcgagc tgtatcccta catcaggaag ctggaaaggg
     8641 agggctacga cgccacccgc taccgcgtcg atctccacgg caagccggcc ttcagcgtgg
     8701 tcagccttat cctgctggcc atcggcatca gtttctccct ccggtcggag cggagcggtg
     8761 gggtggccca gagcatcggt gccggggtcg tcatcggctt ttcctactgg ctggtcttcg
     8821 ccttttccct ctccctgggc cgttccggaa atctcccccc catcctggcg gcctggctcc
     8881 ccaacctgct cttcggcggg gcggcctact acctgatccg ccgggtcaat acctgacccg
     8941 cgccttccct aggggttttg gggtacaccc gagccgcggc ctggtcttgt gattcggctt
     9001 cgtttcggcc gatggagaga tcaccccggg tgacgcacgg cgaccatgca aagccctgag
     9061 cacccgcagc gtcgccttca ataccggtag tggtcgggtt tgaagggacc gcgcgtcggg
     9121 acgccgagat agtcggcctg ggccttggta agcttggtca gcttgacgcc gaggcggtcc
     9181 agatggaggc gggccacctc ctcgtcgaga agtttcggca gggtgtaaac cccgggtcga
     9241 tagcgcttct tggccagttc catctgggcc aggcactggt tcgtgaagct gttgctcatg
     9301 acgaagctcg ggtggccggt ggcgcacccc agattgacga gccgtccctc cgccaggacg
     9361 atgatggaac gcccggaggc gagggtccat ttatcaacct ggggtttgat ggtctccttt
     9421 cggcatcggg ggttgccctc caggtaattc atggcgatct cgctgtcgaa gtggccgatg
     9481 ttgcagatga tcgcctcgtc cttcatccgc tccatatgag ccccggtgat gacgtcgcag
     9541 caacccgtgg ccgtgacgaa gatgtccgcc gtcgccacca cctcttccat ggtctggacc
     9601 tcgtaacctt ccatcgccgc ctgaagggcg cagataggat ccacttccgt aacgatgacc
     9661 cgggcgccga agccccgcat ggactgggcc gaacccttgc caacgtcgcc gtagccgcag
     9721 atcaccacca ccttgcccgc caccatgatg tccgtggccc gcttgatgcc gtccgccagg
     9781 gactcgcggc agccgtagag gttgtcgaac ttggatttcg tcaccgaatc gttgacgttg
     9841 atggcgggaa agaggagttc cccgcgctgc tgcatctggt agaggcgatg gacgcccgtt
     9901 gtcgtctcct ccgacacccc gatgatcccc gcggcgatgc gctgccagcg ctgggggtcc
     9961 cgtttgtatc cccgggccag acgttccatg atgatggcga attccttgtt ttcctgcttt
    10021 tcattcaata tttccggatg cttttccact ttcacgccct ggtgaaccat aagggtggca
    10081 tcgccgccgt catccacgag caggtcgggc cccgatccgt cgggccaggt catggcctgt
    10141 tcggtgcacc accagtaatc ctccaggctc tcccccttcc aggcgaagac cgccgctgaa
    10201 cccgccgcgg cgatcgccgc ggcggcgtga tcctgggtgg agaagatgtt gcaggaggcc
    10261 cagcgcagat ccgccccgag ttccttcagg gtctcgatga gcatggccgt ctggatggtc
    10321 atgtggaggc tccccatgac ctttttgccc tggaggggtt tttggggccc gtattttttc
    10381 cggaccgcca tgagccccgg catctcgttt tccgccaatt tcatctcctt tcgcccccag
    10441 gccgcgagat ttatatcggc caccttgtaa gccagtttcg gatcgatctt cttgtaagcc
    10501 acaactcctc cttgaggtga taatttttgt tgaataaccg cacgggcctc ttaggccggc
    10561 ccgcgtgagg ctccgccgcc ggggacaggc ggcaagggac caaaactccc cgccccggct
    10621 tcccatgccg gcgcgatcaa cggcccgccg cctgccgcag cgcctcgacc cggtcggtct
    10681 tctcccaggg atagtcatcc aggaagagag tccgggcgat cttcttgtag gtccggctgt
    10741 tgagaagatc gaaggcctcg atcatccccc ggggggtcag atcgaagatt tccgaaacga
    10801 tcttccccag tttgtcgtct tcgagctgac cgccgccaaa ggtgttgacg tatatggaaa
    10861 tgggccgggc aatgccgatg gcataggcga tctgaacgga acatcttttg gccagcccgg
    10921 cggcgacgat atttttggcc acataacggg ccccgaaggt ggcggaacag tccacctttt
    10981 ccggcgtctt gttgatgatc gacccgccac caatctgcgc ccccggatag cctccgtaga
    11041 gctgcaccac cagttttctc ccggttaccc cggaatccgc ggcgctgcac gaattgaccg
    11101 cctgccattc ccccgtggga t
//
LOCUS       DAOSMF010000065        11021 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_6791_length_11021_cov_24.942641, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000065 DAOSMF010000000
VERSION     DAOSMF010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 11021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11021
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6791_length_11021_cov_24.942641"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="PLT63_07300"
     CDS             <1..183
                     /locus_tag="PLT63_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98566.1"
                     /translation="RRTAALLAKVFTGPVEIYPFLPSGGAAPEEGARRLIDEIAARIA
                     DRMAALTTPPANDQSL"
     gene            321..1352
                     /locus_tag="PLT63_07305"
     CDS             321..1352
                     /locus_tag="PLT63_07305"
                     /inference="COORDINATES: protein motif:HMM:NF002558.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOD98567.1"
                     /translation="MMFSAVLVNPPGGDPGVYLEMKYRRRALMFDLGEIYNLPPRKIL
                     KIEYICISHTHMDHFIGFDHLVRLCLGRNREIALYGPPGFLGQIEGRLGAYNWNLVEE
                     YVNDFDIVVTEVSPEGWQRTRRFSCRHGFRPEGEGEERPFDGLLCADRYFSLRGAFLD
                     HRTPCLAFRLEERRRINIRKDALAELGLPTGSWLNDLKDCLIDDRPPDFPVRIRWKEA
                     GGMKRERQVPLGELVEKIVRITPGRKIAYITDAVYSAANARTILALAGGVDILFIEAP
                     FLHADADQAARKHHLTARQAGLLAGTAGVKRMIIFHFSPKYRGREEELYDEAAAAFRE
                     NRGRRETPV"
     gene            complement(1368..1802)
                     /locus_tag="PLT63_07310"
     CDS             complement(1368..1802)
                     /locus_tag="PLT63_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98568.1"
                     /translation="MQSSRSNDRRHGEKGLPKNTGSDPPGPGGAAAAAGSAPGAEDPS
                     RLTANEHAAYPDLLRGQLEKIPGLRKVSFYTQEFGNWHKETGELLGKVFGGNSHVCQA
                     FQAVMFTPLFLSCQGGDTVFAEAYQQGLTEVHALLRSCFDRT"
     gene            complement(2025..2438)
                     /locus_tag="PLT63_07315"
     CDS             complement(2025..2438)
                     /locus_tag="PLT63_07315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00640.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HOD98569.1"
                     /translation="MSSNSTIRILMAKLGEGYEDAVGKLCIAFREAGYEVVYVENQRP
                     EAIVASALQEAVDHIGITTLPGARIEDIARVMELLKKEDLSYVRVTAGGFLDYNDIHK
                     VKEAGVVEFFPKGTSISELITWAKANITLTDDWSK"
     gene            complement(2478..2897)
                     /locus_tag="PLT63_07320"
     CDS             complement(2478..2897)
                     /locus_tag="PLT63_07320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOD98570.1"
                     /translation="MRFQSWECQYSTSAGTDVIDITGDVAAKVREAGIGTGQVLISVP
                     GSTASITTIEYESGVVRDLVEALERIAPASIPYRHDARWGDGNGYAHVRAALLGPSVV
                     VPISGGRLVLGTWQQLVLLDFDNRPRQRRIFLQVLGI"
     gene            3334..3660
                     /locus_tag="PLT63_07325"
     CDS             3334..3660
                     /locus_tag="PLT63_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HOD98571.1"
                     /translation="MVPDDENTLPRYDGDTETDTFVYLDEPAMFRVILHNDHYTTMDF
                     VVEVLMRIFQKPAAEAMKIMMNVHRHGHGVCGVYTYDIAVTKVNRVHAMAKSRGYPLK
                     SSYERV"
     gene            3680..5938
                     /gene="clpA"
                     /locus_tag="PLT63_07330"
     CDS             3680..5938
                     /gene="clpA"
                     /locus_tag="PLT63_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HOD98572.1"
                     /translation="MKISEELDSIFRAAYTEAKLRSHEYLTPEHILFAALFYESSSEI
                     IRSCGGDVKSLQNRLEDFFATKLPKQKRETDPVQSASFQNVIERAVWHTTSAQKGELD
                     IGDILISIFDERESYAAFFLHREGITRLDLLNYISHGITASPREKPGTGEGEKKDPAG
                     PKENKILATFTTELVQKARSGELDPLIGREDILERTMQVLCRRAKNNPVHVGEAGVGK
                     TAITEGLAQLIAQDKVPKPLKSAKIYSLDMGALIAGTRFRGDFEERLKKVIAELQKIQ
                     NVVLFIDEIHTIVGAGAASGGAMDASNILKPVLTSGKMKCIGATTYDEYRKCLEKDRA
                     LSRRFQSIDIPEPSVEDTVNILKGLKDRYESYHQVAYTEEALRAAAELAARHINDRYL
                     PDKAIDVMDEAGAKTRLYRGEDGGTVTISPEDVERIVARMARIPEKSVSSSEGARLRF
                     LDMELKTRIFGQDQAIDRVVQAIRRSRAGFGDVNKPVASLLFAGPTGVGKTELARQLA
                     LSLGIPLHRYDMSEYQEKHSVSRLVGAPPGYVGFEEGGLLTEAIRKTPHAVLLLDEIE
                     KAHPDIFNTLLQVLDYATLTDNNGRKADFRNVIIIMTSNAGARDIGRVEIGFGERRIN
                     RQAVSEAIKKIFSPEFRNRLDAMVTFNGLTRADVISIVKKQLREFQQQLTEKGVTLET
                     TDAAVEWLAEKGYSDDFGARQIARLIQEEVKDFFVTEVLFGRLMAGGRVCVDVADDKI
                     DLAILDACIPAH"
     gene            5916..6701
                     /gene="aat"
                     /locus_tag="PLT63_07335"
     CDS             5916..6701
                     /gene="aat"
                     /locus_tag="PLT63_07335"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HOD98573.1"
                     /translation="MPVFRLTERLMFPPPRLAADGGLLAVGGDLTPERLLLAYRQGIF
                     PWFSEGEPILWWSPDPRFVLFPDHLRISRNMQRILRKGVFAVTFDTVFADVVTACREQ
                     RRRGEGTWITPEMVSAYIRLHQLGLAHSVEAWREGRLVGGLYGVSLGRCFFGESMFSR
                     VANASKVALVTLVGKLAVRGFQVIDCQVYTEHLRRLGAEPIPRESFLALLDRTLAAPT
                     FRGSWRFLNEDSEVTPGRGKAPSTVLLASRRRLNTPYFVQNLQ"
     gene            6766..7329
                     /locus_tag="PLT63_07340"
     CDS             6766..7329
                     /locus_tag="PLT63_07340"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOD98574.1"
                     /translation="MKKKEMDFFTALYEVASVINASLEPARVLEKIVQSVVQAMDLKA
                     ASIRLLDDREKKLVLGGAYGLSEDYIHKGPILVDESGVDRKALRGRTIYLRDAQTDKG
                     FQYGEKARAEGIKSVLVTPLMVEKKAIGVLRGYSDRIREFSERELKFLQAVSHLSAIA
                     IENARLHQLLQVRCDLMAAHKYRIDDN"
     gene            7343..8353
                     /gene="gap"
                     /locus_tag="PLT63_07345"
     CDS             7343..8353
                     /gene="gap"
                     /locus_tag="PLT63_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOD98575.1"
                     /translation="MIRLGINGFGRIGRQVLKAVMERYPQTLQVAAVNDLFDVETNAH
                     LLKYDTNYGRFPGEVTVKKDCFVINGQQIRNFAFRDPASIPWDDEGVDIVVESTGLFL
                     TGPKAAAHLQGGARKVIITAPAKEEDITIVMGVNHKQYVPQKHHIISNASCTTNCLAP
                     PVLVIHQVFGIEKGMMTTVHSYTNDQRILDLPHKDLRRARAAAQNIIPTTTGAAKALA
                     FVIPDLAGRFDGYSLRVPTPTVSIVDFTAELKTKTTTDYLRKVLVMASRKSLKGIMAC
                     EEERLVSTDFRGNEHSSVIDLEFTVVLRENLAKVVAWYDNEWGYSCRVADLANFIVQK
                     GL"
     gene            8360..9694
                     /locus_tag="PLT63_07350"
     CDS             8360..9694
                     /locus_tag="PLT63_07350"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOD98576.1"
                     /translation="MKIRILVIDDDEVACEFLQEALNRAGYEVRYFTSAKAALADNLS
                     RYDLLMSDIRMPGIDGLEFLKRVREQWPELPMILMTAYGSLETTMNALRLGAWDYISK
                     PFSPEAIREMVKKVLDVRDLRQKRGRGGQKIREDHQFIGSSATMVDFYKQVARVADAE
                     ASVLIEGESGTGKELTARSLHQLSIRRDKPFVVVHCGAIPDTLLESELFGYEKGAFTG
                     ADHSHAGLIESAQDGTIFLDEITEMSTGLQAKLLRFMQDGEVRRLGGHTVRHVSLRVL
                     AAANRNIDEEIKKGTFRSDLLYRFVVRLHIPPLREHKEDLPQLVESLLKKLGSSSVRI
                     SDEVMELFLAYDWPGNVRELQNVLQQTLLLSPFGLILPEYLPERFQRRKKTDIQGLTP
                     LEEAEREQIRQCLQQVAWNQTRAAQALGIDRKTLRTKIQRYGLIRGIDNHVS"
     gene            complement(9851..>11021)
                     /locus_tag="PLT63_07355"
     CDS             complement(9851..>11021)
                     /locus_tag="PLT63_07355"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD98577.1"
                     /translation="AIETMKPQYLSINTEGGPALALLYPDVSEKDRSIHFVGMISFSQ
                     TRTEILGRAWVLLLYSSIGLLSVIFIVLLVSYDRLIGRSLRTIIKTIDEFQEGNYRHR
                     IDLKRPDELGHLATHFNDMATEIEQVLARNRELTQHLENRVQEETIKVVELQNQVNQL
                     QRLTAMGHLTATLAHDLGTPLHSIAGLARLLLERGGWPPDVSRKLELIVQQTQRLHAV
                     IQNIRRVTRPPEPHFEATTVEELLNETLPLVEPLIQKANIVLKVQAPEGLPPLHVDRY
                     RIQTALMNLIQNATEALAARENGAITVTAALDAPNRRIAISIGDNGPGIPPELLARIY
                     EPFFSTHVEERLRGLGLVIVQDIMKIHSGALEISSSPGGGTTASIYLPVYDQAAG"
BASE COUNT         2210 a         3259 c         3326 g         2226 t
ORIGIN      
        1 cggcgaactg cggcgctgtt ggcaaaggtc tttaccggtc cggtggagat ataccctttc
       61 cttccctcgg gcggggcggc gccggaagag ggggcgcgac ggcttatcga cgaaattgcc
      121 gcccggatcg ccgaccgcat ggcggcattg accacgccgc cggcaaatga tcagtccttg
      181 taacgaagat agtgtatgct tgccgggaca acctcccgaa gcttcctttc tttctctgag
      241 gaaaaagtca aagtgaaacc gtcactgcct cggccggcgt atcgcgccgt caaccgctct
      301 tcttcccgtc tggaggggtg atgatgttca gcgctgtcct ggtgaatcct cccgggggcg
      361 atcccggggt ctatctggaa atgaaatacc ggcgccgggc cctcatgttc gatctggggg
      421 agatctacaa cctgccgccc cggaagatcc tgaagatcga gtatatctgc atcagccata
      481 cccatatgga tcacttcatt ggtttcgacc acctggtgcg cctctgcctg ggaaggaacc
      541 gggagattgc cctgtacgga cccccgggat ttcttggcca gatcgagggg cgcctagggg
      601 cgtacaactg gaacctggtg gaggaatatg ttaatgattt cgatattgtc gttacggagg
      661 tgagtccgga gggctggcag cggacccggc gtttttcctg ccgccacggc ttccggccgg
      721 agggggaagg ggaagagcgg ccctttgacg gcctcctgtg tgccgaccgt tatttcagcc
      781 tccggggggc ttttctggat caccggacgc cctgcctggc cttccgcttg gaagagcggc
      841 gccggatcaa catccgcaaa gacgcccttg ccgaactggg gcttcccacg ggatcctggc
      901 tgaacgatct caaggactgt ctcatcgacg accgcccgcc ggatttcccc gtgcggatcc
      961 ggtggaagga ggcggggggg atgaagcggg aaagacaggt gcccctgggg gaactggtgg
     1021 aaaagatcgt ccggatcact ccgggccgga agatcgccta cattaccgac gccgtttaca
     1081 gcgccgccaa cgcccggacg attctcgccc tggccggggg cgtggatatc ctcttcatcg
     1141 aagcgccctt cctccacgcc gatgccgacc aggccgcccg taaacatcac ctcacggccc
     1201 gtcaggccgg actgcttgcg gggaccgccg gggtgaagcg gatgatcatc ttccattttt
     1261 cccctaaata ccgaggtcgg gaagaggaac tgtacgacga agccgcggcg gcattccggg
     1321 aaaaccgggg ccgccgggag acacccgtgt agggaagtgg cgcccgttca ggttctgtcg
     1381 aagcaggacc gcaacagtgc atgcacctcc gtcagcccct gctggtaggc ctcggcaaag
     1441 acagtgtcgc cgccctggca actgaggaac agcggggtga acataacggc ctgaaaagcc
     1501 tggcagacgt gggaattccc gccgaaaacc tttcccagca gttccccggt ttccttgtgc
     1561 cagtttccga actcctgggt atagaaggaa acctttctca atccagggat tttttccagt
     1621 tgtccccgca ggaggtccgg ataggcggcg tgttcattgg cggtgaggcg cgacggatcc
     1681 tcagcgccgg gtgctgaccc ggcggcggcc gcggccccac ccgggccggg cggatcgctt
     1741 cccgtattct ttgggaggcc cttttcccca tgccgacggt cattgcttcg tgaggattgc
     1801 attgcccctc tttcctcgtg ggcggctgtc accgtgacgc cgccttttca tctgaaacac
     1861 tcgccaaact agctgtattc attgataata tctaatatgc ttccctgaag gtgcccggtt
     1921 gggaaaaggg tcggaaattt tccttaccct tggtgacgga acgccgcctt gcctctggag
     1981 ctttttactt cgctgtcccc atcatgactt ataacgaatt catcttattt ggaccagtcg
     2041 tccgtcaggg tgatgtttgc tttagcccag gtgatgagtt ccgatatgga ggttcccttg
     2101 gggaagaact ccaccacccc ggcctccttt accttgtgga tgtcgttgta atccaggaag
     2161 ccgccggcgg tgacccgcac ataggataga tcctcctttt tgaggagctc catcacccgg
     2221 gctatgtctt cgatccgggc gccggggagg gtggtgatgc caatatggtc caccgcttcc
     2281 tggagggccg aggcgacgat ggcctcgggg cgctgattct ccacgtatac gacttcgtag
     2341 cccgcctccc ggaaggctat gcacaatttt cccaccgcgt cctcgtaacc ttcccccaac
     2401 ttcgccatca ggatcctgat cgtgctgttg ctgctcatgt tgtccccctt attccctctg
     2461 atatacgaga gttatattta tattcccaat acttgcagga aaatacgacg ctggcggggg
     2521 cgattgtcga agtcaaggag aacgagctgc tgccaggttc ccaggaccag acggccgccg
     2581 ctgatgggaa cgaccaccga cggccccagg agggcggccc ggacgtgggc gtagccgttg
     2641 ccgtctcccc agcgggcatc gtgccggtaa gggatgctgg cgggggctat gcgctccagg
     2701 gcttcgacta ggtcccggac gacgccgctt tcatattcga tggtggtgat ggacgccgtg
     2761 gaacccggta cggaaatcaa gacctggcct gtgccgatcc ctgcctcccg taccttcgct
     2821 gcaacgtcgc cggtaatgtc gatgacgtct gtgccggccg atgtggagta ttgacactcc
     2881 cagctctgaa atctcatgtt ccctctctgt ttgccgggta gatctttatc gtaagaacca
     2941 caaaggattt tttccggaag ccgggccaaa gcgaggcgtc gacaaacgcc cttcctgatt
     3001 gtgccatgac ccttgttttc cagaatatga aggatttatt tttattgccc ccggatctgt
     3061 tatagttttt tctgccgagt gtcggtttcc cggcggtggg caattggccg ggtccgcgct
     3121 gcttgacttc tatatcaaaa ggaagaaaaa aagtcacctt ccgccgtcca cccgtccact
     3181 gaaaggccaa aaactaatga atcaatctgg agatacccaa taaacgctca atattcatgg
     3241 agaatatggc tgtctacttt ctttgcatag accccttgca tttctggcaa caattcataa
     3301 aatagaactt cagttcggtg caggagtaag cccatggttc cggatgatga aaacacactg
     3361 ccccggtacg acggggacac cgaaacggac acgttcgtgt atctggatga gccggccatg
     3421 ttccgggtga tccttcacaa cgatcattac accaccatgg attttgtcgt ggaggtgctc
     3481 atgaggatat ttcaaaagcc ggccgcggag gccatgaaga taatgatgaa cgtccatcgg
     3541 cacggtcacg gcgtctgtgg cgtctatacc tacgatatcg ccgtgaccaa ggtaaaccgg
     3601 gtccacgcca tggcgaagag ccgcggctac cccctcaaga gcagttatga aagggtttga
     3661 agcacatcag gagtaagtca tgaaaatcag tgaagaactt gattccatat tccgggcggc
     3721 atacacggag gcgaagctcc gttcccacga atatctcacg ccggagcata tcctcttcgc
     3781 ggccctgttc tatgaatctt ccagcgagat catacggtcc tgcggtgggg atgtgaagag
     3841 tctccagaac cgtctggagg attttttcgc caccaagctg ccgaaacaga agcgggagac
     3901 cgacccggtg cagagcgcca gttttcagaa cgtcatcgaa cgggcggtct ggcacaccac
     3961 ctcggcccag aaaggcgaac tcgatatcgg cgacatcctg atctccatct tcgacgagcg
     4021 tgaatcctac gccgcctttt tcctccaccg cgagggcatc acgcgcctgg atctgctgaa
     4081 ttacatctcc cacggtatta cggcgtcccc gcgggaaaag cccgggacgg gggaggggga
     4141 gaagaaggac ccggcgggcc cgaaggagaa caagatcctg gccaccttca cgacggaact
     4201 ggtgcagaag gcccggtcgg gggaactgga tcccctcatc ggccgggagg acatcctcga
     4261 gaggaccatg caggtcctct gccgccgcgc caagaacaac cccgtccacg tcggggaagc
     4321 gggggtaggc aagacggcca ttaccgaggg cctggcccaa ctgatcgccc aggacaaggt
     4381 tcccaaacca ctgaaaagcg ccaagatcta ttccctggac atgggtgccc tcatagcggg
     4441 gacccggttc cgcggggatt tcgaggaacg actgaaaaag gtgatcgccg agctccagaa
     4501 gatccagaac gtcgtcctgt tcatcgacga gatccatacc atcgttggtg ccggcgccgc
     4561 ctccgggggg gcgatggatg cctcgaacat cctcaaaccg gtgcttacct ccggaaagat
     4621 gaagtgcatc ggcgctacca cctacgatga gtaccggaaa tgtctggaaa aggaccgggc
     4681 cctgtctcgg cgctttcaat ccatagacat ccccgaaccg tcggtggagg atacggtgaa
     4741 tattctcaag ggcttgaagg accgttacga atcctatcac caggtcgcct atacggaaga
     4801 agccctgcgg gcagccgcgg aacttgcggc ccggcacatc aacgaccgct accttcccga
     4861 caaggccatc gacgtcatgg acgaagcagg ggccaagacc cgcctgtacc gtggggagga
     4921 cgggggaacc gtcaccattt cccctgagga tgtggagcgt atcgtggccc ggatggcccg
     4981 gattcccgag aaatccgttt cctcgtcgga aggggcgcgc ctccgctttc tcgatatgga
     5041 gctgaagacc cggatcttcg gccaggatca ggccatcgac cgggtcgtcc aggccatccg
     5101 ccgctcccgg gcgggcttcg gggatgtgaa caagcccgtg gcgtctcttc tgttcgccgg
     5161 tcccaccgga gtcggaaaga cggaactggc ccggcaactg gccctctccc tgggtatccc
     5221 cctgcaccgc tacgacatgt cggaatatca ggaaaaacac agcgtatcca ggctggtcgg
     5281 cgccccgccc ggctatgtgg gcttcgagga ggggggactc ctgacggagg ccatccggaa
     5341 gactcctcat gccgtcctcc tcctcgacga gatcgagaag gcccatccgg atatcttcaa
     5401 taccctcctg caggtactgg actatgcgac gctcacggac aacaacggcc gtaaggctga
     5461 tttccgcaat gttatcatca tcatgacgtc caatgccggg gcccgtgaca tcggccgggt
     5521 ggagatcggc ttcggggaac ggcgtatcaa ccgccaggcg gtaagcgaag ccatcaagaa
     5581 gatcttcagc cccgaattcc gcaaccgcct ggatgccatg gtgaccttca acggactgac
     5641 gcgggcggac gtgatcagca tcgtgaagaa gcagctccgg gaattccagc agcaactgac
     5701 ggaaaaggga gtcaccctgg agacgaccga cgccgcggtg gagtggctgg cggaaaaggg
     5761 ctattccgat gactttgggg cccgccagat cgcccggctt atccaggagg aggtcaagga
     5821 cttcttcgtc acggaggtcc tcttcggccg actcatggcc gggggccggg tctgcgtcga
     5881 tgtggcggac gacaagatcg acctggcgat cctcgatgcc tgtattccgg ctcactgaac
     5941 gactgatgtt ccccccgccc cgcctcgcgg cggatggggg cctactggcc gtcggcggcg
     6001 atcttacccc tgagcgcctc ctgctcgcct atcgtcaggg gatctttccc tggttttccg
     6061 aaggggaacc aatcctctgg tggtctccgg atccccgttt cgttctcttt ccagatcacc
     6121 tccggatctc ccgaaacatg caacggatac tgagaaaggg cgtctttgcc gtcactttcg
     6181 ataccgtctt cgcggacgtg gtgacggcct gccgggagca gcggcggcgc ggggagggga
     6241 cctggatcac cccggagatg gtgtccgcct atattcgcct ccaccagtta ggcttggccc
     6301 actccgtgga ggcctggcgg gaggggcggc tcgtcggcgg cctctatgga gtctctctgg
     6361 ggaggtgctt tttcggggag tccatgttca gcagggtcgc caacgcctcc aaggtggccc
     6421 tcgtcaccct ggtgggaaaa ctggccgtcc ggggcttcca ggtcatcgac tgccaggtct
     6481 atacggaaca tctccgccgc ctgggggcgg agccgatacc ccgggagagc tttctggccc
     6541 tgctggaccg gaccctcgcc gcccctacct tccggggttc ctggcgcttt ctcaacgagg
     6601 attcggaggt gacgccggga aggggaaaag cgccttccac cgtcctgctc gccagtcgtc
     6661 gccgcctgaa caccccctat tttgtccaaa accttcaata aataattgtc atcatgagaa
     6721 aaaaatatta aggattcagt aaagaccatt ggaggaggtg tggggatgaa gaagaaggag
     6781 atggattttt ttaccgcttt gtatgaagtg gccagcgtga tcaatgcctc cctcgaaccg
     6841 gcgcgggtct tggaaaagat agtccagtcg gtggtccagg ccatggacct caaggccgcc
     6901 agcatcagac tcctcgacga ccgggaaaaa aaactcgtcc ttggcggcgc ttacgggctc
     6961 tccgaggatt atatccacaa gggaccgatc cttgtagacg aaagcggggt ggacaggaag
     7021 gccctccggg gccgcacgat ctatctccgg gacgcccaga cggacaaggg cttccaatac
     7081 ggggagaagg cccgggcgga aggcatcaag tcggtcctgg tgacgccgct gatggtggaa
     7141 aaaaaggcca tcggcgtctt gcgggggtat agcgaccgga tcagggagtt cagcgagagg
     7201 gaactcaagt tcctgcaggc ggtgtcccat ctgagcgcca tcgccatcga aaacgcccgc
     7261 cttcaccagc tcctccaggt gcgctgcgat cttatggcgg cccacaaata ccgcatcgac
     7321 gacaattgag gaggtaagaa gaatgattcg cctgggtatc aacggattcg gaagaattgg
     7381 acggcaggtc ctcaaggccg tcatggagcg gtatccccag accctgcagg tggcggcggt
     7441 aaacgacctc ttcgatgtgg agaccaacgc ccatctgctc aaatacgata ccaattacgg
     7501 ccgctttccg ggagaagtga cagtgaagaa ggactgtttc gtcatcaacg gccagcagat
     7561 aagaaacttc gccttccggg accctgcgtc cataccctgg gatgacgagg gggtggacat
     7621 cgtagtggag agtacgggtc ttttcctgac cgggcccaag gcggcggccc atctccaggg
     7681 tggggcgagg aaggttatca tcaccgcccc cgccaaggag gaggatatca ctatcgtcat
     7741 gggggtcaac cacaaacagt atgttcccca gaagcaccat atcatctcca acgcctcctg
     7801 caccaccaac tgcctcgccc cgccggtgct ggtgatccac caggtcttcg ggatcgagaa
     7861 gggtatgatg acgacggtcc attcctatac caacgaccag cggatcctcg atctgcccca
     7921 caaggatctg cgccgggccc gggcagcggc ccagaacatc atccccacca ccaccggggc
     7981 ggccaaggcc ttggccttcg ttattcccga tctggccggg cgcttcgacg gctattccct
     8041 ccgcgtgccc acgcccacgg tttctatcgt ggacttcacg gcggagttga agactaagac
     8101 cacaacggat tacctccgca aggtccttgt catggcgtcg cggaaatccc tgaaggggat
     8161 catggcctgc gaggaggaac ggctcgtttc cacggacttc cggggtaatg aacattcctc
     8221 cgtaatcgat ctggagttta ccgtggtcct ccgggaaaat ctggccaagg tcgtggcctg
     8281 gtatgacaac gagtggggct attcttgccg ggttgctgat ctggccaatt ttatcgtcca
     8341 aaaaggtctg tagggagaga tgaaaatccg tatcctggta atcgacgacg acgaggtggc
     8401 ctgtgaattt ctccaagagg ccctgaaccg cgcgggttat gaagtgcggt acttcacttc
     8461 ggcgaaggcc gccctggctg acaatcttag ccgttacgat cttctcatgt cggatatccg
     8521 tatgccgggg atcgacggac tggaatttct taagcgcgtc cgggagcagt ggccggagtt
     8581 gcccatgatc ctcatgacgg cctatggctc cctggagacg acgatgaacg ccctgcgtct
     8641 gggggcctgg gattacatca gcaagccctt ctcgcccgag gccatccggg agatggtgaa
     8701 aaaggtcctc gacgtacgtg atttgcgtca gaagcgcggc cggggggggc agaagatccg
     8761 ggaagaccat cagttcatcg gctcatcggc gacgatggtg gacttctaca agcaggtggc
     8821 acgggtggcc gatgccgagg ccagcgtcct cattgagggg gagagcggta cgggaaagga
     8881 acttacggcc cgatccctcc accagttgag catccgccgg gacaagcctt tcgtcgtcgt
     8941 tcattgcggc gccatccccg acaccctcct ggagtcggag ctgttcggtt acgaaaaggg
     9001 ggccttcacc ggggccgacc actcccatgc cggattgatt gagtccgccc aggacggcac
     9061 catctttctc gatgaaatta cggagatgtc cacgggactc caggcaaagc tgctgcgctt
     9121 catgcaggac ggcgaggtcc gccgcctggg cggccacacg gtgcgccatg tatccctccg
     9181 ggtcctggcg gcggccaacc gaaacatcga cgaggagata aaaaaaggca ccttccgctc
     9241 cgatctcctc taccgtttcg tcgtccgtct ccacatcccg cccctccggg agcacaagga
     9301 ggatctgccc caactcgtgg agtccctgct gaaaaagctg ggatcgtctt cggtgcggat
     9361 ctccgatgag gtcatggagc ttttcctcgc ctatgactgg ccgggtaatg tgcgggaact
     9421 gcagaacgtc ctccagcaga cgctgctctt gtccccgttc gggttgattc tgccggaata
     9481 tcttcccgaa cggttccaac ggcggaagaa gacggacatc caggggttga cgccgctgga
     9541 ggaggcggaa cgggagcaga tccgccaatg ccttcagcag gtggcctgga atcagacccg
     9601 ggcggcccaa gctctgggta tcgaccgcaa gaccctccgc accaagatcc agcgctatgg
     9661 attgataagg ggcatcgata accacgtctc ctgaaaccgc gtaccgagcg gacggtttcg
     9721 atcatgtcgc ggcggcttcc tgatttggat gcaggttgtg actttcccgt cggcggcgat
     9781 cggcgggact cgtcgcttca tgtgcctgcc ggggcgggag acgggatgcg accggccgcc
     9841 ttacgacggt ttaccccgcc gcctgatcgt acacgggcag gtagatgctg gccgtcgttc
     9901 ccccaccggg gctgctggag atctccaggg cgccggaatg gattttcatg atgtcctgga
     9961 cgatgaccag ccccaggccg cgcagccgtt cttccacatg ggtgctgaag aatggttcgt
    10021 agatccgggc caacagctcc ggggggatgc ccggcccgtt atcgccgatg gaaatggcga
    10081 tccgcctatt cggggcgtcg agagcggcgg tgacggtgat cgcgccgttt tcccttgcgg
    10141 ccagggcctc ggtggcgttc tggataaggt tcatcagggc ggtctgaatc cggtaacgat
    10201 ccacatggag cggcggcagg ccttccgggg cctgaacctt caagacgatg ttcgcttttt
    10261 gaatcagggg ttccaccagc ggcagggttt cgttgaggag ttcctccact gttgtagcct
    10321 cgaagtgggg ctccggcggc cgggtgaccc gccggatgtt ctgaatgacc gcatggaggc
    10381 gctgggtctg ctgaacgatg agctccagct tgcggctcac gtccggtggc cagccgcccc
    10441 tttccaggag gagcctcgcc aggccggcga tggagtgcaa gggcgttccc agatcgtggg
    10501 ccagggtggc ggtcagatga cccatggcgg taagtcgttg gagctggttg acctgattct
    10561 gcaactccac gaccttgatc gtctcctcct ggacgcgatt ttccagatgc tgagtcagct
    10621 cccggttcct tgccaggacc tgctcgatct ccgtggccat gtcattgaaa tgcgtggcca
    10681 ggtgccccag ttcatccggt cttttcaggt cgatgcggtg ccggtagttt ccttcctgaa
    10741 attcgtcgat cgtcttgatg atggtccgca gcgaccggcc gatcagccgg tcgtaactga
    10801 cgagaaggac aatgaagatg acgctcaaca ggccgatgct gctgtaaagc agcaacaccc
    10861 aggcccggcc cagaatctcc gtccgtgtct gggaaaaact tatcatccct acgaaatgga
    10921 tgctcctgtc cttttccgac acatcggggt agagcagcgc cagggcgggg ccgccttcgg
    10981 tattgatgct cagatactgg ggcttcatcg tctcgatggc g
//
LOCUS       DAOSMF010000066        10884 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_6893_length_10884_cov_22.694616, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000066 DAOSMF010000000
VERSION     DAOSMF010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 10884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10884
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6893_length_10884_cov_22.694616"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            274..1161
                     /gene="xerD"
                     /locus_tag="PLT63_07360"
     CDS             274..1161
                     /gene="xerD"
                     /locus_tag="PLT63_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HOD98578.1"
                     /translation="MNDYLATYLDYLTVERGASPHTLDAYRRDLRRFIGFMTERGRED
                     IGTVTPEDVLAFIAYLRGLDLSANSVNRSLAAIRGFYRFLSKERKTLASPVDRLATGK
                     TWMLLPNVLSIHDIDRLLAQPGMNSPAAMRDSAILEMLYATGLRVTELIELTVHGIDW
                     QAGFVVVMGKGRKERVTPMSRTAYDLLRRYVEEARGALLKGRMSNSLFVNKSGGKFTR
                     QGLWKLVKKYARAAGMEEKVHPHTFRHTYATHLLEGGADLRAVQVMLGHADIATTQIY
                     MHITRDRLKSVHKRFHPRG"
     gene            1168..3204
                     /locus_tag="PLT63_07365"
     CDS             1168..3204
                     /locus_tag="PLT63_07365"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD98579.1"
                     /translation="METDLIRKRKPERYRQYRQYRKLVFAAAAGLGAVAILAGIIWGL
                     AAIFPKNMPPVSSGWQRVKNFTSFTVLGKQPQLYYLDIEKNGKVYRITPRDHFEVTYK
                     DEFIITGVASDDLRGKGITVDIDGTGRRNDFQVLMKGIEFVDRLMKQVGAGGETHTGG
                     DYRIHVRYRDREIGTIPLKIVLLPQDWLRHAQGSASERHQIESLRKAIAANPNDMGIR
                     KVLAGIYYRQGMFKAAGDEYERILKINPHDGGALKELARCHQSQKNYEQAVKALERLS
                     AVQPNDASVFTSLGNTYAAMGAWSKAASSYQQSLRLNSEDGKVHFLLGKAYEAGNRMK
                     EAIGSYQAALSKNPTVDEIVTALANANLKAGNNDEAIKWYNELIKRQPRNATAYANLG
                     LAYGNKKQPGKEMENYRKALSLNPKDPVIQYNLAAALQKSGKAPEAAKHYRKALELRP
                     DDADALIRLADMEMKNKNYDQAIRLYEKALPKVKNKASLYANLGFAYGELKVYKSSAE
                     NYERALKAGLRNAQLYYNLGFTYAKIGKRKESIANYEKFAAAQPTENVLNILMEAYMR
                     EKRYDEAIKTARRLLTITKKKGRAYANLGRAYGAKGDADLAIEMYRQALKYDREDDAV
                     YAGLGEAYEKKALYQEALKAYQTANQLNPENQRAARRIPALKIRIIQEKHQGEE"
     gene            3236..4066
                     /gene="kdsA"
                     /locus_tag="PLT63_07370"
     CDS             3236..4066
                     /gene="kdsA"
                     /locus_tag="PLT63_07370"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HOD98580.1"
                     /translation="MKTIRIDRITIGAGRPLALIAGPCVIEDERMTRRIAAFLKKLTN
                     RLNIPFIFKASYDKANRTSLQGYRGPGAAQGLEILAGIRADYRLPVLSDVHRFEEIEA
                     AAAVLDVVQIPAFLCRQTDFVMTVARKARTINVKKGQFLAPWDMKNVIEKVEAAGNEN
                     LLITERGTSFGYNNLVTDMRSLPILRNFGYPVIFDATHSVQLPGGMGTSSGGQREMIP
                     FLARAAVAAGVDGIFFEVHPEPETARCDGPNSLHLAELEELLAALVKIDRLVKSSIRE
                     "
     gene            4063..4602
                     /locus_tag="PLT63_07375"
     CDS             4063..4602
                     /locus_tag="PLT63_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HOD98581.1"
                     /translation="MNMEITPELRERIIPLRLLILDVDGVLTDGRIVMNDHGEESKFF
                     DVKDGHGLKVLMRCGLEVVWITGRKSHVVEYRAKDLGVQEYHQLIWNKVEVYEEILVR
                     KGLAPAEVAYVGDDIVDIPLLKRVGFAVAVNDAVSEAKQAAHYVTSRRGGRGAVREVC
                     DLILQGQEKWDRLLARYFS"
     gene            4604..5155
                     /gene="lptC"
                     /locus_tag="PLT63_07380"
     CDS             4604..5155
                     /gene="lptC"
                     /locus_tag="PLT63_07380"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HOD98582.1"
                     /translation="MKKTLPAITAVLVLMAASILFYLWEQGRKEADKALKILPDHVDM
                     QVRDVLYTDVSADGAKWEIRAKTVTYGRQDNLAVFDQVAVMMIMTDGRTYRLTAEEGR
                     YWTATKDMEVKGDVLVISDQGDHITTDKLHYTEKDKLLHTDAPVMLENEKIILRGKGM
                     RLYTKTRRIELLSQIKATMKTGR"
     gene            5160..5726
                     /locus_tag="PLT63_07385"
     CDS             5160..5726
                     /locus_tag="PLT63_07385"
                     /inference="COORDINATES: protein motif:HMM:NF015901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HOD98583.1"
                     /translation="MTVNIRCMPIWLGFLSVVFGLLLPVPDVSGQALDKKLPREPIQI
                     TSNRLEAFNDQRLVVFAGNAVAVQGRKVIRADKLLVYYKKGADDGKAEPGTALGQGGD
                     LDRIEAKGKVRITELNRVVTGEDAVYYQDDQKIVVSGNALMREGDSVVRGERITVLLN
                     ENRGLVEGTAGKRVTATIYPGENSKKKK"
     gene            5750..6466
                     /gene="lptB"
                     /locus_tag="PLT63_07390"
     CDS             5750..6466
                     /gene="lptB"
                     /locus_tag="PLT63_07390"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004091254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOD98584.1"
                     /translation="MLAAKGLVKVYGGRRVVNNINLAIEAGEVVGLLGPNGAGKTTTF
                     YMIVGLIRPDGGNIFLDGDDISGSPMYLRAKKGLNYLPQEPSIFRKLTVEENIMAILE
                     TLDMDHGQRRERMESLLGELDLTALAGNPAYSLSGGERRRVEITRALVTSPKFILLDE
                     PFAGIDPLAVADIQKIIYKLKAKGIGVVISDHNVRETLSVCDRAYIVNEGSILVEGDP
                     DTIASCETAKKIYFGEDFCL"
     gene            6479..7942
                     /gene="rpoN"
                     /locus_tag="PLT63_07395"
     CDS             6479..7942
                     /gene="rpoN"
                     /locus_tag="PLT63_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOD98585.1"
                     /translation="MAFELKQNLKLSQQLIMTPQLQQAIKLLQLSRLEMVDMINQEME
                     ENPLLEEVSIGEDMIEEAPAETDSLRSLETDEIKPVDHTVEITGEGDGREDFDWNNYL
                     EDYGPMGVTYQRAEDEGASWDNILTKTTSLTDHLMWQLKLSPMTETEMRVGEQIIGNL
                     DQNGYLQASIEEIADQEHAAADFVTRVLKTVQSFDPPGIAARDLTECLLIQARLLGET
                     NPLVETIIRDHLKDLEMKNYPQIARKLKVEIDEVVQAVMVISNMDPKPGSAFQEEKVQ
                     TVIPDVYVYKSGDDYKIVLNDDGMPRLRISNFYREIMAGMDGGRDVDTGKRYIKERVQ
                     SATWLIKSIQQRQKTIYRVAESIVNHQRNFFEKGINYLKPLVLRDIAMDVEMHESTIS
                     RVVTNKYMQTPAGLFELKYFFSSGLSKGNGENIASKSVKEEIRKLIAEENPRKPLSDN
                     EIVMMLKASGIKIARRTVAKYREMINILPSSRRKKFF"
     gene            8022..8561
                     /gene="raiA"
                     /locus_tag="PLT63_07400"
     CDS             8022..8561
                     /gene="raiA"
                     /locus_tag="PLT63_07400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOD98586.1"
                     /translation="MKISVTFRNTDGEEWFKNYLEEKLGKLKKYLDRPVDVRVVLSVE
                     KFRNVAEINLTDNGMIVNTREEAKEMVLAIDEAVEKIERRLKKHREKMRVHKGAGVRS
                     GEDIMESTPEGALEDIHEPRFVETRKVVLNPMSHEDAIMELETSKNLFIIFRDTATEN
                     VAVIYRRDDGNYVLVETNS"
     gene            8578..9039
                     /locus_tag="PLT63_07405"
     CDS             8578..9039
                     /locus_tag="PLT63_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOD98587.1"
                     /translation="MKIAEILKKDFIIEQLSSRTKRDVLEELAAVILGGRPGYHAEET
                     VNVLLGREKLGSTGIGDGIAIPHGKLTIIDELTISFGISQEGIDFNAMDGRPVHLFFL
                     LLAPENAAGQHLKMLAKLSKMLKDAAFRNDLLTARSRDEIMQVIINKDEFC"
     gene            9139..9606
                     /locus_tag="PLT63_07410"
     CDS             9139..9606
                     /locus_tag="PLT63_07410"
                     /inference="COORDINATES: protein motif:HMM:NF019118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphatase C-terminal
                     domain-containing protein"
                     /protein_id="HOD98588.1"
                     /translation="MKHIHGCLIQWMGKGILLIGESGAGKTTCALEMVRRGGIWVADD
                     LVELTIGADRRPLGKAHKKIRGLAWLPSKGIFSVTRAFPSFHAIPECRIDFVVELIKK
                     LEKTKEKYLTVGEIWKAMDLRAAFLSVMVDDNVQRTVPRIIRRLRCREGSKEG"
     gene            9608..10465
                     /gene="rapZ"
                     /locus_tag="PLT63_07415"
     CDS             9608..10465
                     /gene="rapZ"
                     /locus_tag="PLT63_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HOD98589.1"
                     /translation="MQIVVITGMSGSGKSTALRALEDLGFFCVDNLPIVLLPKFLKIQ
                     SDPSKMISKVAMVMDLREQGFLEKYPRIFKRLREQGYVIEILFLDAGDEALLHRFSET
                     RRSHPLAVKGSIMEGIAMERQKLAPLKLMADRIIDSTSSNVHQLKDVIQRIFLSPGAE
                     KRLIIHVTSFGYRYGLPVDADIVLDVRFLPNPYFVDSLKKHDGHHPAVRDYILASDEG
                     RRFIEKLFALMSFLLPLYEKEGKVRLHIALGCTGGKHRSVAMANELAAYFSDKGYNIQ
                     ANHRDIGKS"
     gene            complement(10664..>10884)
                     /locus_tag="PLT63_07420"
     CDS             complement(10664..>10884)
                     /locus_tag="PLT63_07420"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOD98590.1"
                     /translation="FSLYAHLSSIDVKTGQEVKKGVVLGKSGLTGLAGGDHLHFSILV
                     GGQFVNPTEWWDPHWIQDNITKKMDVSF"
BASE COUNT         2862 a         2668 c         3130 g         2224 t
ORIGIN      
        1 gggaattgaa ccgcgggaaa ttctttcccg atgattatga aaccagctgg gcaaatatag
       61 cacaggtcgg aagactccga ctataaaaga gttcctacga tcgatcacta aggtggactc
      121 tttcctttga ctgggatcgc gatggccggg aatacagccg gcaagaaatt tccggcagca
      181 tgagattgcc gcatgtccat ttctccggtc accgtcggtt acggcaggaa aaaagctttg
      241 cgtacttatt cgatttgagc tacaaaaaaa gatgtgaatg attacctggc gacatatctc
      301 gactacctga cggtggaaag aggcgcctcc ccacatacgc tggatgccta ccgacgcgat
      361 ctgagacgtt ttatcgggtt catgacggaa cggggacggg aagacatcgg aacggttaca
      421 ccggaagatg tcttggcttt cattgcctat ctccggggtt tggatctatc cgctaattcc
      481 gtgaaccgat ccctggcggc catccgcggt ttttaccgtt ttctgtccaa ggagaggaaa
      541 acgctggcca gccccgtgga tcgtctggcc acgggcaaaa cctggatgct tctccccaac
      601 gtgctcagca tccatgatat cgatcgcctc ctggcgcaac cagggatgaa cagcccggcg
      661 gcgatgagag attcggcgat tctggagatg ctctatgcaa ctggattaag ggttacggaa
      721 cttattgaat tgacggtcca cggcatcgat tggcaggcgg gctttgtggt ggtcatggga
      781 aagggaagga aggaacgggt aacgcccatg agccggacgg cttacgatct cctgaggcgt
      841 tatgtggagg aggcgcgcgg cgcactcctg aagggacgga tgagcaacag tctgtttgtc
      901 aataaatcgg gcgggaaatt tacccggcag ggactatgga agctggttaa gaaatacgcc
      961 cgggccgccg ggatggaaga aaaggtgcat ccccacacct ttcgccatac ttatgccacc
     1021 catctgttgg aggggggagc ggatctgagg gcggtgcagg ttatgctcgg tcatgccgac
     1081 atcgccacca cccagatcta catgcatata actcgagatc gactgaaaag cgtccataaa
     1141 cgatttcatc cccgtggttg agtgatcatg gaaaccgatt tgatccgcaa aagaaaaccg
     1201 gaacgttaca ggcagtatag gcagtataga aagttggtat tcgcggcggc ggccggcctc
     1261 ggtgcggttg ctatcctggc cggaattata tggggattgg cggccatttt tccgaagaat
     1321 atgccacctg tgtcttccgg atggcagcgg gtaaagaatt tcaccagctt cacggttttg
     1381 ggcaagcagc cgcaattata ctatctggat atagaaaaga atggcaaggt ttatcgaatc
     1441 acgccccgag accatttcga ggtgacctac aaagacgagt tcatcattac cggggtagcc
     1501 agcgacgatc tccgagggaa aggcataacc gtcgatattg acggaaccgg acggcgcaac
     1561 gatttccagg ttctcatgaa agggatagag ttcgtggaca ggctgatgaa acaggtaggg
     1621 gccggcggag aaacgcatac cggcggcgat tatcgaatcc atgtccgata ccgtgatcga
     1681 gaaatcggca cgatcccctt gaagatcgtt ttgcttcccc aggattggct gagacacgca
     1741 cagggatcgg cgagcgaacg gcatcagatc gagtccctga gaaaggcgat tgcggcaaat
     1801 cccaacgaca tggggatcag gaaggttctg gcggggattt attaccgcca gggcatgttc
     1861 aaggcggcgg gcgacgaata cgagcgcata ttgaagatca atccccatga cggcggcgcc
     1921 ctcaaggaac tggcacgttg ccatcaaagc caaaaaaact acgaacaggc ggtcaaggcc
     1981 ctcgaacgtt tgtcggcggt acagcccaac gatgcctcgg tattcaccag tctgggaaac
     2041 acctatgcgg ccatgggtgc gtggtccaaa gcggcatcat catatcagca gtctctgcgc
     2101 ctcaactcgg aggacggcaa agtacatttc ctcttgggaa aggcatacga agcgggcaac
     2161 cgcatgaaag aagccatcgg ttcctaccag gctgctctga gcaaaaatcc gaccgtggac
     2221 gaaatcgtca ccgccctggc caatgccaat ctcaaggcgg gaaacaacga cgaggccatc
     2281 aagtggtaca atgaactgat caaaagacag ccgcgcaatg ccaccgccta tgcaaatctt
     2341 ggcctggctt acggaaacaa gaaacaaccc ggcaaggaaa tggaaaatta ccggaaggcg
     2401 ttgagtttga atccgaagga tccggttatc caatacaatc tagctgccgc cctacaaaaa
     2461 tcaggaaagg cgccggaagc cgccaaacat taccgcaagg ccctggaact tcgacccgac
     2521 gacgccgacg ccctgattcg actggcggac atggaaatga aaaacaagaa ttacgaccaa
     2581 gcgatcagac tctacgaaaa ggccttgccg aaggtaaaaa acaaggcctc tttgtatgcc
     2641 aatctcggct ttgcctatgg tgaactgaag gtttacaaat catcggcgga aaattacgag
     2701 cgggcactca aagctggatt acggaatgcc cagctttact ataacctcgg ctttacctat
     2761 gccaaaattg gcaaaaggaa ggaatcgatc gccaactatg agaaatttgc cgccgcccag
     2821 ccaacggaga atgtgctgaa tattctcatg gaggcttata tgcgggagaa gcgttatgac
     2881 gaggcgatca agacagcgag aagactcttg acgattacca agaagaaagg gcgggcatat
     2941 gccaacctgg gccgggccta tggcgccaaa ggggacgcgg acctggctat cgagatgtac
     3001 cggcaggcgc tgaagtatga ccgggaggat gatgccgttt atgccggtct tggggaggcc
     3061 tatgaaaaga aggctcttta tcaggaggcg ttgaaggcct atcaaaccgc caatcagctc
     3121 aatcctgaaa accagcgggc ggcaagaagg atcccggctc tgaagatcag gatcatccag
     3181 gaaaaacacc aaggggagga gtgaaaatgg aaaacgaggg acgcggcggg cggaaatgaa
     3241 aaccatccgg atagacagaa tcaccatcgg cgccggtcga ccgctggcgc tcatcgccgg
     3301 tccttgtgta atcgaggatg agcggatgac caggcggatc gcggctttcc ttaaaaaact
     3361 caccaaccgg ctgaacatcc ccttcatttt taaggcctct tatgataagg ccaaccgcac
     3421 ctcgctacag ggctatcggg ggccgggagc agcgcagggt ttggagattc tggcggggat
     3481 cagggcggat tatcggcttc ccgtcctgtc tgacgtccat cgcttcgagg aaatcgaggc
     3541 cgcggcggcc gtccttgatg tggtgcagat ccccgctttc ttatgtcgcc agacggactt
     3601 cgtcatgacg gtggcgcgga aagcccggac aatcaatgtc aaaaaggggc agtttctcgc
     3661 tccctgggat atgaagaacg tcatcgaaaa ggtggaagcg gcgggaaatg aaaatttgtt
     3721 gatcacggaa cgaggcacgt cctttggtta caacaacctg gtaaccgata tgcgatcgct
     3781 gcccatcttg aggaatttcg gctatcccgt gatcttcgat gccacccaca gcgtccagtt
     3841 gccgggagga atggggacat cgtcgggagg acagcgggaa atgattcctt ttttggcccg
     3901 ggccgccgtg gccgccggcg tggacggaat attcttcgag gttcatccag aaccggagac
     3961 agcccgttgt gatggtccca attcattaca cttggcggag ttggaggaat tgctggccgc
     4021 cctcgtgaag attgaccgcc tggtgaaaag cagtattagg gaatgaacat ggaaataacc
     4081 ccagaactga gagagagaat aataccccta cgcctcctca tcctcgatgt ggacggggtc
     4141 cttaccgacg gtcgcatcgt tatgaacgat catggcgagg agtcgaaatt cttcgacgtc
     4201 aaggacggcc acggcttgaa ggtcctcatg cggtgcggtc tggaggtcgt atggataacc
     4261 ggccggaagt cccacgtagt cgagtaccgg gcgaaagatc tgggtgtgca ggagtatcac
     4321 caattgatct ggaacaaggt ggaggtctat gaggaaattc tggtcaggaa gggattagct
     4381 ccggcggagg tggcctatgt gggtgacgac attgtcgata tccccctgtt gaaacgagtc
     4441 ggtttcgccg tagccgtcaa tgacgccgtt tccgaggcaa aacaagccgc ccactatgta
     4501 accagccggc ggggaggacg gggtgccgtc cgtgaggtat gtgacctgat cctccagggc
     4561 caggagaaat gggaccgtct cctggcgaga tatttttcct gaaatgaaaa aaactctgcc
     4621 ggcgataacg gcagtattgg tattaatggc cgcctccatc ttgttctacc tctgggagca
     4681 gggaaggaag gaagccgata aggccctgaa gatcctgccc gatcatgtgg atatgcaggt
     4741 gcgggacgtt ctctacaccg atgtttccgc cgacggcgcc aaatgggaaa tcagggccaa
     4801 gacggtcacc tatggacgcc aggacaactt ggctgtcttc gatcaggtag cggtaatgat
     4861 gatcatgacc gacggacgga cataccggct caccgcggag gagggacgct actggacggc
     4921 caccaaggat atggaggtca agggggatgt attggttatt tccgatcagg gggatcacat
     4981 taccacggat aaactacatt atacggaaaa agacaaacta ttgcatacgg atgcaccggt
     5041 aatgctcgaa aatgaaaaga taatattgag aggaaagggt atgagactct acacgaaaac
     5101 ccgtcgcatc gaattgttgt cacaaataaa ggccaccatg aagacaggaa gataagaaaa
     5161 tgaccgtaaa tattagatgc atgccgatct ggctgggatt cctctccgtc gttttcggtc
     5221 tgctgcttcc ggtcccggat gtgtcggggc aggccctaga taaaaagctt cccagagagc
     5281 ccattcagat tacctccaat cggctggagg ccttcaacga ccaacgcctc gtcgtttttg
     5341 ccggtaacgc cgtggccgtg cagggccgaa aggtgatccg cgccgataag cttcttgtat
     5401 attacaagaa aggggcggat gacgggaaag cggagccggg tacggccctc ggccagggcg
     5461 gggatctcga tcggatcgaa gccaagggta aggtgcgcat caccgaactt aaccgggtgg
     5521 ttacgggaga agatgccgtt tattatcaag atgatcaaaa gatagtggtg agcggcaacg
     5581 cgttgatgcg ggaaggggac agtgtcgtcc ggggagaacg aattacggtc ctcctcaatg
     5641 aaaacagggg tcttgtggaa ggtaccgcag gcaaaagggt gacggcgacc atctatcccg
     5701 gtgaaaatag caagaaaaag aaatagtaaa acggcatcgg gggaatagca tgctggcggc
     5761 gaagggactg gtcaaggtat atggagggcg gcgggtggtg aacaacatca acctcgccat
     5821 cgaggcgggg gaggtggtcg gactcctcgg tcccaacggc gccggcaaga ccacaacctt
     5881 ttatatgatc gtcggattga ttcgccctga tggcggcaac atctttcttg acggggatga
     5941 cataagcgga agccccatgt atctccgggc aaaaaaaggc ttgaattatc ttccccagga
     6001 accgtcgatt ttccgaaagc tcacggtaga ggagaatatc atggcgatcc tggaaaccct
     6061 ggatatggat cacgggcagc gccgcgagcg catggagtct ctcctcgggg agctggacct
     6121 gacggccttg gcaggaaatc ccgcctattc cctttcgggg ggggagcggc ggcgggtgga
     6181 gattacccgg gccctggtaa cttcgccaaa attcatcctt ttggatgaac cctttgccgg
     6241 tatcgacccc ctggcggttg cagatataca aaagatcata tataaactga aggcaaaggg
     6301 aatcggcgtg gtcatttcgg atcataatgt ccgtgaaacc ctttcggttt gtgatcgcgc
     6361 ctatatcgtc aacgaaggaa gcatcctggt agagggggat cctgacacga tcgcctcctg
     6421 cgagacggcg aaaaagatct atttcgggga agacttctgc ctgtaaggaa acttttgtat
     6481 ggcttttgaa ctcaaacaga atctgaaact ctctcaacag ttgatcatga cgccgcaact
     6541 gcagcaggcg ataaaactcc tgcagttgtc gcgattggag atggtggaca tgatcaatca
     6601 ggagatggag gagaacccgc ttctggagga ggtctccata ggcgaggata tgattgagga
     6661 agccccggcg gaaaccgatt ccctgcggag cctggagacc gacgagatca agccggtgga
     6721 tcacaccgtc gagatcacag gggagggcga cggacgggaa gacttcgact ggaacaatta
     6781 tctggaagat tacggaccga tgggtgttac ctaccagcgg gccgaggacg agggggcctc
     6841 ctgggacaac atcctgacca aaacgacgtc cctcaccgat catctaatgt ggcaactgaa
     6901 gctctcgccg atgacggaaa cggagatgag ggtgggagaa cagataatcg gcaacctcga
     6961 tcaaaatggg tatctccagg cgagcatcga ggagattgcc gatcaggaac acgcagccgc
     7021 agactttgtg acccgggtcc taaaaactgt acagtccttt gatcccccgg gtattgccgc
     7081 tcgggatctt acggaatgtc ttttgatcca ggcgcgcctg ctgggtgaaa ccaatcctct
     7141 ggtggaaacc atcattcgtg atcacctcaa ggatctggaa atgaagaact atccccagat
     7201 cgccagaaag ctcaaggtag agatcgatga agtggtgcaa gccgtcatgg tcatcagcaa
     7261 catggatccg aagccgggaa gcgcctttca ggaggaaaag gttcagaccg tcatccccga
     7321 tgtctatgtg tacaagtcgg gggatgacta taagatcgtc ctgaacgatg acggcatgcc
     7381 gcggttgcgg atcagcaact tctaccggga gatcatggcg ggaatggacg gcggccggga
     7441 cgtcgatacc gggaagaggt acatcaagga acgggtccaa tcggcgacct ggttgatcaa
     7501 gagcatccag cagcgccaga aaaccatata cagagtcgcg gaaagcatcg ttaaccatca
     7561 gcgcaatttc ttcgaaaagg ggatcaatta tctcaagccc ctggtcctcc gtgatattgc
     7621 catggatgtg gaaatgcacg aatcaacaat cagcagggtg gtgacgaata agtacatgca
     7681 gacacccgcc gggctttttg aacttaaata ttttttcagc agtggccttt cgaagggcaa
     7741 cggagaaaac atcgcctcca agagcgtcaa ggaggaaatc cgcaaactga tcgctgagga
     7801 aaatcccagg aagccattga gcgacaacga aattgtcatg atgctgaagg cctcgggaat
     7861 caaaatcgcc cggcggacgg tggcaaagta tcgagaaatg attaatattc tgccatcatc
     7921 acggcgtaag aagttcttct gaagaaaaaa ggggttgact tttcccggcg acaccaatat
     7981 agcacaagac ctttttacga gtaagacaac gggaggaaag aatgaagatc tctgtgacct
     8041 tcaggaatac agacggggaa gagtggttca aaaattacct ggaagagaag ctcggtaaac
     8101 tgaaaaaata cctcgatcgt ccggtggatg ttagggtggt gctgtcggtg gaaaaattcc
     8161 ggaatgtggc ggaaattaat ctcacggaca acggcatgat cgtcaatacc agggaagagg
     8221 caaaagagat ggtgctggcc atcgacgagg cggtggagaa aatcgagcgg cggttgaaga
     8281 aacaccgcga aaagatgcgg gtacacaagg gcgccggtgt cagatccggc gaggatataa
     8341 tggaaagtac accggaaggg gcactggaag atatccacga accccgattc gtggagaccc
     8401 ggaaggtagt cttaaatccc atgtcccatg aagatgcgat tatggaactg gagacctcga
     8461 aaaatctctt catcatcttc cgtgataccg cgacagaaaa tgtcgccgtc atatatcggc
     8521 gggatgacgg caattacgtc ctggtggaaa ccaacagcta aatccggaaa gatgcagatg
     8581 aagatcgcgg aaatactcaa aaaggacttc ataatcgaac aattgtcatc ccggaccaaa
     8641 cgagatgtcc tggaggaact ggccgccgtc atcctgggcg gccggccagg ttatcacgct
     8701 gaggagacgg tgaatgtcct tttggggaga gaaaaactgg ggagtaccgg aatcggagat
     8761 ggcatcgcca tcccccatgg gaaactaacc atcattgatg aattgaccat ttcctttgga
     8821 atcagtcaag aagggataga tttcaacgcc atggacgggc ggcccgtcca tctgttcttt
     8881 ctccttctcg cgccggaaaa cgcggcggga cagcacctga aaatgttggc taaactgtcg
     8941 aaaatgctga aagatgctgc ttttcgcaac gatctcctca ccgcccggtc cagggacgag
     9001 atcatgcagg tcatcatcaa caaggatgaa ttctgctgat cggtccggat cgcagttgcc
     9061 cctcttcgga aaaagcggtt tgttatcgac gtgatttgcc cttccagcag tgaataaccc
     9121 cgggaagtgg acgggaccat gaagcatatt cacggttgcc ttatccaatg gatgggaaag
     9181 gggatcctgc tgatagggga aagcggcgcc gggaagacga cctgcgccct ggaaatggtc
     9241 cggaggggag gcatatgggt agccgacgat cttgtggaac tgaccatcgg cgccgaccgg
     9301 cgtcccctag gcaaagccca taagaagata cgcgggcttg cctggttgcc gtcgaaaggc
     9361 atcttcagcg tcacccgtgc atttccatct tttcacgcca ttccggagtg ccggatcgat
     9421 tttgttgtcg aactgatcaa aaaacttgaa aaaacgaagg aaaaatattt aactgtgggc
     9481 gagatctgga aggccatgga tttgagggct gcttttttgt cagtcatggt cgatgacaac
     9541 gtccaacgca ccgtgccaag gatcatccgg cgactccgat gtcgggaggg cagtaaggag
     9601 ggataagatg cagattgtgg tgatcaccgg catgtcgggt tccggtaaga gtaccgctct
     9661 gcgggctttg gaggatctcg gttttttttg cgtggataat ctgccgatcg tcctgctgcc
     9721 taaattcttg aagatccagt ccgatccatc caaaatgatc tccaaagtgg ccatggttat
     9781 ggatttgcgg gagcagggct tcctggaaaa atacccccgg atcttcaaac gcctccggga
     9841 gcagggctat gtcatcgaga tcctgtttct ggatgccggg gacgaggcct tgctgcatcg
     9901 gttcagtgaa acgcggcgca gtcatcccct ggccgtgaag gggtcgatta tggagggcat
     9961 cgcaatggag cggcaaaaac tggctccgct gaagctgatg gccgacagga tcatcgacag
    10021 cacctcctcc aacgtacatc agttgaaaga cgtcatacag aggatcttcc tgtcacccgg
    10081 ggcggaaaaa cggctgatca ttcacgtgac atccttcgga taccgctatg gcctcccggt
    10141 ggatgcggac atcgtcctgg acgttcgctt tctccccaat ccctattttg tggattcctt
    10201 gaaaaaacac gacggtcatc atccggcggt gcgggattac atcctggcct ccgatgaggg
    10261 acggcgattt attgaaaaac tctttgccct catgtctttt ctattgcccc tgtatgaaaa
    10321 ggaaggcaag gtacggctac atatcgccct tggctgtacc gggggaaaac atcgttccgt
    10381 cgccatggcc aacgaactgg ccgcgtattt ctccgacaag ggctacaata tccaggccaa
    10441 ccaccgggac atcgggaaaa gctgaaggac ctggggagat agtggcgatt tccgaacaat
    10501 gaaagttata tgtgcttgca gaaatctcgc ggctctcttc cttacactac caaacttaat
    10561 cttaacccct tgcccgctga ccaaacccta aaaaaagagg tggaaaatca tttttacgaa
    10621 gtcgtgagta tttactcacc gtaaccaatc gcagccctgc gatttaaaaa gagacatcca
    10681 tcttcttggt aatattgtcc tggatccagt gagggtccca ccactccgta ggattcacga
    10741 attgaccccc aaccaggatg gaaaagtgca aatgatcgcc gccagccaga ccggtgaggc
    10801 cgcttttccc gagaaccacc ccttttttga cctcctgccc ggttttcaca tcgatgctcg
    10861 acaagtgggc atacaggctg aaga
//
LOCUS       DAOSMF010000067        10863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_6907_length_10863_cov_19.110381, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000067 DAOSMF010000000
VERSION     DAOSMF010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 10863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10863
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6907_length_10863_cov_19.110381"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            252..599
                     /locus_tag="PLT63_07425"
     CDS             252..599
                     /locus_tag="PLT63_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98591.1"
                     /translation="MPWENVGSCGSGQMPYDKDWIVQCYELALSYLNFIVGTAPEGCE
                     LGIMWHEHELGDYPSIGLFWDYPQSDPPWKFISKCESLLERFDEAVNWSEINPDAVAE
                     DMEEDSEYEDDEY"
     gene            941..1237
                     /locus_tag="PLT63_07430"
     CDS             941..1237
                     /locus_tag="PLT63_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOD98592.1"
                     /translation="MTIIMNKSDLIAALAAKENFFEKNAKDVINLIFDGFTNALKNGD
                     RIEIRGFGSFSVRNYESYRGRNPKTGKKIEVSEKRLPYFKVGRELKKRVDAPNK"
     gene            1567..1842
                     /locus_tag="PLT63_07435"
     CDS             1567..1842
                     /locus_tag="PLT63_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98593.1"
                     /translation="MKKTLGTLVAAIVVVLCTGTFALAEYAAAGVGNFPYFQLGCLIV
                     GGMVIISLKHKYEKMYTGEVAGVFALYTVLIALFTNPVIEGIKNLVG"
     gene            1881..2120
                     /locus_tag="PLT63_07440"
     CDS             1881..2120
                     /locus_tag="PLT63_07440"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HOD98594.1"
                     /translation="MAARQETRKALYHKYNPVMIVSAYGFVIAIASFLFLYLGFKIDQ
                     YFGTAPNFMFGMFFLALFLSIGRLYQEAWLKRKEV"
     gene            2784..4283
                     /locus_tag="PLT63_07445"
     CDS             2784..4283
                     /locus_tag="PLT63_07445"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HOD98595.1"
                     /translation="MSLSFHDIKSHLWKCAEILRGSAVDRTDWKAYILPLLFFKRICD
                     VWDEETAEAASLFGDVEPIDYPEIHRFIVPESCHWRDIRETPSNIGAALSRAMREIER
                     ANPDTLYRVFGAADWGNRELLTDAILKDLIEGLSEASLGNAAVSSDILGDAYEYLIGK
                     FADITKRKKAGEFYTPRSVVRMMVDILAPKAGESIYDPACGTGGMLLGIVEHVQRAGG
                     DPRTFFGKIYGQEKNLTTASIARMNLVLHGIEDFLIVREDTLRQPAFTDSSTGGLSTF
                     DCVIANPPFSLKEWGREIWESDPWGRTAFGLPPESYGDYAWVQHMVASMAERTGRMAV
                     VLPQGALFRKGAEGDIRQKLLEKDLIEAVIGLAPNIFYGTGLAPAVMVLRRVKPADRK
                     GKVLIVDASSLFRKGRAQNYLDPEHAQQIVEWVGTFRDVEDRAKVIGLAEIRAEDWTL
                     NISRYVLPPVADNIPSLPEAVAAFKEALTEARTAEARLREVLIEGGWLK"
     gene            4280..5803
                     /locus_tag="PLT63_07450"
     CDS             4280..5803
                     /locus_tag="PLT63_07450"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HOD98596.1"
                     /translation="MTSTAPHLSQQELESYLWGAAKLLRGLVDAGDYKQYIFPLLFFK
                     RLSDVWDEEYEAALADTGDASYARDTADDRFAVPEGAHWSDVRSASRDVGRALLNAFR
                     AIEAANPERLSGVFGNASWTDKAQMPDSILKNLIENFSQHTLNLSAVPEDELGNGYEY
                     LIKQFADDSGHTAQEFYTNRTLVHLMTQMLEPKAGESIYDPTAGTGGMLISALAEVKR
                     QGGDVRTLGLYGQEVIHITAAIARMNLVLHGVEDFQVVAGNTLSNPAFIERDRLRTFD
                     VVLANPPYSIKRWNREAWTSDPWGRNFLGTPPQGRADYAFFQHILKSLDPKTGRCAIL
                     FPHGVLFRNEEADMRRKLVELDLLECVLGLGPNLFYNSPMEACVVICRSCKPPDREGK
                     VLFIDAVHEVARERAQSFLKLEHQKRILAAYQTFADDPGFAKVATKKEILDKDGNLSI
                     PRYVRPVGNGNNNGAGDVPSLKKMWAAFEASGREFWEQMDALVDMLDGVVAGEAEDA"
     gene            5796..6989
                     /locus_tag="PLT63_07455"
     CDS             5796..6989
                     /locus_tag="PLT63_07455"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HOD98597.1"
                     /translation="MPEKLKQGWTRVAFGDVVQLCRERSGAPLDDGFDRYVGLEHLEP
                     GDLKIRRWGNIADGTTFTNVFRTGQVLFGKRRAYQRKVALADFEGVCSGDIYVFKTKN
                     ETLLSELLPFICQSDGFFNHAIGTSAGSLSPRTNWESLASYEFALPPPEEQRLIAEVL
                     LAHLETRKGFQNAVRCAERLRMSLLRWHFSDCGSECLVALRSLVDNGDVELKTGPFGT
                     VLAAKEYQTSGWPIVNPTDMKSGRICHEGGPCVDDATANRLDVYRLREGDILLARKAD
                     LSKAVLAATEHTGWIGGSDTIRLRCTSSKVLPGYLHLALQTDGAARMLYSYAHGTVMP
                     GLNEKALYRISINLPDGPEQQAVVDHFRLIEARERELLEREVACAVLGRVASEQLLTP
                     ERSLM"
     gene            6986..9955
                     /locus_tag="PLT63_07460"
     CDS             6986..9955
                     /locus_tag="PLT63_07460"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019812 - type I site-specific
                     deoxyribonuclease complex [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HOD98598.1"
                     /translation="MTNFTESNTVEAYIRDRLAGPVRITRPNEIQGQVASFGKPRLGW
                     NFASPMDVPRQPQEVMVERWVREALIRLNPEIAAQPDRADDVLYKLRAIIMGVRSDGL
                     VKGNEEFAAWLNGERSMPFGPNGEHVTIRLLDFSDLTQNKYVVTTQYTIRAGATEKRA
                     DLVLLINGIPLVVIEAKTPVRASQSWLDAAIQIHEDYERNVPELFVPNLLSIATEGKE
                     FRYGSIGLPLDLWGPWRIESGIATPALQEVAKGVDSLLRPHVLLDLLANFTCYATDKK
                     KHRIKIVARYQQFDGANKIVDRVVAGYPKKGLIWHFQGSGKSLLMLFAARKLRLHPAL
                     KNPTVMIIVDRIDLDTQISSTFYAADTPNLVKADSRTELQRLLGQDVRKIIITTIFKF
                     GEADGVLNDRSNIIAMVDEAHRTQEGDLGRKMRDALPNAFLFGLTGTPINRADRNTFY
                     AFGADEDSAGYMSRYGFEESVRDGATKPLHFEPRLLEMHIDKEDIDQAFREMTGSLSD
                     LDRDQLAKTAAKMAVLVKSPERIQKICTDIARHFQENVAPNGFGAQVVTYDRESCVLY
                     KKALDEYLPPEVSDIVMTVNSGETEYAAYNRDRESEEKLLDRFRDPADPLKVLIVTSK
                     LLTGFDAPILQAMYLDKPLRDHTLLQAICRTNRPYGETKTHGLIVDYLGVFDDVAQAI
                     QFDEEGITRVVTNINELKAKLPEALQKCLTYFAGVDRTVTGYEGLIAAQNCLPDNNTR
                     DRFAADYSYLSRLWEAISPDPILSPHEADYRWLSQVYDSVKPSTGSGRLLWHALGAKT
                     IELIHQHVHIDAIRDDLETLVLDADLLEAVLGTPDPNKKAKEIEIKVSRWLRKRLHDP
                     RFKALGERLEELKVRHERGLLTSVEFLKELLNLARDVVTAERAQPPVEREVQGKAALT
                     KLFEEVKNPETPIIVARVVNDIDEIVRYVRFDGWQNTHAGEREVKKALRKTLFKYRLH
                     QDQELFNRAYGYIRQYY"
     gene            complement(10140..10706)
                     /locus_tag="PLT63_07465"
     CDS             complement(10140..10706)
                     /locus_tag="PLT63_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008591948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HOD98599.1"
                     /translation="MLIGYARVSTDDQNLDLQRDALTQAGCERITEDRLSGAKAERPG
                     LKLALDIARDGDVLVVWRLDRLSRSLKDLIEMVTLLESKGISLKSLHEAIDTSSSSGK
                     LIFHIFGALAEFERNLIRERTQAGLTAARARGRRGGRPRALDLNKRILAVKLYDKKEH
                     TVSQICQMMGISKPTLYKYIEAEREVTS"
BASE COUNT         2670 a         2786 c         2936 g         2471 t
ORIGIN      
        1 agctcttgaa actattgcag accaataggg atctgaattt cctgatgtcg ctgcaagaaa
       61 acgacctgac agagctactg attgccgtca gggagagaac cgaagatgcg agggaaggga
      121 ggtaaaacaa accggggagg atagagtaca attttgagga tgaactcata aacttaacct
      181 gaaaggtgca ttttgtccaa tgtggggata cacatctacg cctccgttgg gcatgaaata
      241 cggtgaaact tatgccttgg gagaatgttg ggagctgcgg ttccggacaa atgccatatg
      301 ataaggactg gatagtgcaa tgctacgaac ttgccttgtc atatttgaat ttcattgttg
      361 gcacagcccc ggaagggtgt gaacttggga taatgtggca tgaacatgaa ctcggtgatt
      421 atccgtcaat cggtttgttc tgggattatc ctcaaagcga cccaccatgg aagtttataa
      481 gcaaatgtga aagtctatta gagcgttttg atgaagcagt taattggtca gaaatcaatc
      541 cagacgccgt cgctgaagat atggaagaag atagtgaata tgaagacgat gaatattgaa
      601 aaattgcctt ggcaaagcag attcacgccg tcatcaagaa gacgcgaggc aagggaagta
      661 aatcaaaccg tggaggatag cgggaaattt agtgatcttt taagcctaac ctgaaagatg
      721 ccttttgtcc aatgtgggga tactcatctt tgcctccgtt gtaacgcaca gaatccgtag
      781 cgaactctat gcagcgggag aggacctgat cgccgtcagc gtcgagggcg aacacagtgg
      841 agggactttc aacctatgag tttacgccca tgccgggcgt aggcaagcca gggcctacgt
      901 ttccaacgca cacgcggatg gcaaaaccaa aaggaggata atgacgatca tcatgaacaa
      961 atcagacctt atcgcagctc tggccgctaa agaaaatttc ttcgagaaaa acgcaaaaga
     1021 tgtaataaac ctgatcttcg atggttttac caatgcattg aagaatggcg accggattga
     1081 aatcagaggc ttcggaagct tttcggtgag gaattatgaa tcatacagag gaaggaaccc
     1141 gaagacggga aagaaaattg aggtgagcga aaagaggttg ccatacttca aggtagggag
     1201 ggaattgaag aagagagtgg atgctccgaa caagtaacgg cccgaacaac aagaaaaaat
     1261 agctcaacat cggcaaggcc tggaccacgc tgcggacggg cagctactca ttttcataaa
     1321 gcctgatgcc ttcaatacgc agcttttcaa tcattccatt caacccattt aaagattctt
     1381 tgaaagatcc tcctcaaaca tccccctaat tgccgatcca gatcgcgccg ctttccaata
     1441 atacgcaaca ttatcaaaca tataaaccac caacatctcc cttcaaatca gttggcacgc
     1501 ttcccgcatt atctaaagtc aaacagacgc aaacaaaaaa ataaaaataa atggaggaca
     1561 cagaaaatga aaaagacatt aggaaccctg gtagcggcaa tcgttgttgt tctttgcacc
     1621 ggtactttcg ccctggccga gtatgcggcg gcgggtgtcg gcaacttccc ttacttccag
     1681 cttggatgcc tgatcgtggg cggcatggtg atcatcagcc tgaaacacaa gtacgagaag
     1741 atgtacacgg gagaggtcgc tggtgttttc gctctctaca cggtcctgat cgccctcttc
     1801 accaatccgg tgatcgaagg catcaagaac ctggtcggct aataaacatc atcagcaatc
     1861 tttgaaagga ggaaggggaa atggcagcac ggcaagaaac cagaaaggct ctttaccaca
     1921 agtacaatcc cgtgatgatc gtgagcgcct acggcttcgt catcgccatc gcctccttcc
     1981 tgttcctcta cctgggcttc aagatcgacc agtacttcgg cacggccccc aacttcatgt
     2041 tcgggatgtt tttcctggcc ctcttcctct ccatcggtcg tctctaccag gaggcctggt
     2101 tgaagcgaaa agaggtttga aaaggagccc catcgagaca taaaagacac gtcgtttaaa
     2161 gatctgtgtc ccaaataaaa aaccccctga tgaaccggtc gtcagggggt ttttgttttc
     2221 cggcacacct acccgacagc tatggttttc ccagtttgat tgaaaaaagt ggcggcttcc
     2281 tcttgctcct gctctcacga agaggcctgg cttgctgttc tgttgtaccg tgttcacggt
     2341 ggattctgta tgctcctact gatcacggcg cacacaaggc gacagtggga ggtagtgtcc
     2401 cggaggtccc tctgcaatcc tgcattatag gtcctcttag tccttcctcg aagggagcaa
     2461 gacctccatc ccggcaaatc ctttccaaga ggtgtcactt tttcgttgac aagctctatg
     2521 ggtcgtgaat acgaattatc taacaaaaga ggcataagga gaaatgatcc gcaactccaa
     2581 atctggtgcg ttcaagatcg ttgcgggtga gactgatgcc gaatgcctgg tgcccgatca
     2641 ggttgaatac cagtcaccat aaccaccata ccggaaagag ccaggaagtc gcgaaaggcc
     2701 cttcgcaatg aagagcgata gaggcatcca ttaccacaga atctgcaacg aattctatgc
     2761 agggtcagtc ctttttattt gatatgtcgt taagctttca tgacattaag tcccatctat
     2821 ggaaatgtgc tgagatactc cgtggaagtg ccgtggatcg cactgactgg aaggcttata
     2881 ttctaccctt actttttttt aaacgcatct gcgatgtatg ggacgaagag actgcggaag
     2941 ctgccagtct ctttggtgat gtggaaccca ttgattatcc agaaattcac cgatttatcg
     3001 tacctgagtc atgtcactgg cgagatattc gagaaactcc atcaaacatt ggcgccgctc
     3061 tatcgcgtgc gatgcgtgaa attgagaggg ccaatcctga caccctttac cgcgttttcg
     3121 gagcggctga ctggggcaac cgagagctgc tgaccgacgc cattcttaaa gacctcatcg
     3181 aggggctttc agaagcttct ctcggcaatg ccgccgtcag ttccgacatc ttaggcgacg
     3241 catacgagta cctgattggc aagtttgccg atattacaaa acggaaaaag gccggggaat
     3301 tttacacgcc gcgcagtgtt gtgcgtatga tggtggatat attggcacct aaagcaggtg
     3361 aaagcattta cgacccagcc tgtggaaccg gaggcatgtt acttggaatc gtagagcacg
     3421 ttcaacgcgc aggtggcgac ccgcggactt tcttcggcaa aatctacggc caagagaaga
     3481 atctaacaac ggcctccatt gcccgcatga atctggtgct gcacggtatt gaggactttc
     3541 tgattgtacg tgaagatacg ctccgccaac cggcctttac ggattccagc actggtggtc
     3601 tatccacctt cgattgtgtc atcgccaatc cgcccttttc gctcaaggaa tgggggcggg
     3661 agatatggga atccgatcca tggggccgca cggcctttgg cctgccaccg gaaagttacg
     3721 gggactatgc ttgggttcaa catatggtgg catccatggc ggaacggacc ggtcgtatgg
     3781 ccgtggtgct gccgcagggt gcgcttttcc gcaaaggggc tgagggcgac atccgacaaa
     3841 agctcttaga aaaagatctg atcgaggccg tcataggtct tgccccgaac atcttttacg
     3901 ggaccggcct tgccccagcc gtcatggtct tacgtcgcgt aaagcccgcc gaccgaaagg
     3961 gcaaggtcct gatcgtcgat gcatccagtc tgtttagaaa aggtcgcgct caaaactatc
     4021 ttgacccgga acacgcccag cagatcgtcg aatgggtcgg taccttcagg gatgtggaag
     4081 atcgtgctaa ggtcattggt ctggcggaaa tcagggcgga agactggacg ctaaatattt
     4141 cccgctatgt gctgccgccc gttgcggata acataccatc cctgccagag gcggttgcag
     4201 ccttcaagga agcccttacc gaggcccgta cagcggaggc ccgtctgcgc gaggttttga
     4261 tcgaaggggg ctggcttaaa tgacctctac cgcgccgcat ctaagtcaac aggaactcga
     4321 atcctatctg tggggagcgg ccaagctgct ccgtgggctg gtggatgccg gcgattacaa
     4381 acagtatatc tttccgcttc tcttcttcaa gcggctatct gatgtttggg atgaggagta
     4441 cgaggctgcc ctcgcggaca ccggcgacgc ttcgtatgcc cgtgacaccg ccgatgaccg
     4501 ctttgccgtc cccgaaggcg ctcactggtc cgatgtgcgg tccgcctctc gcgacgtggg
     4561 gcgagctttg ctcaacgcct ttcgagctat tgaggccgcc aatcccgaac gtctctccgg
     4621 tgtgttcggc aatgcctcct ggacggacaa ggcgcagatg cccgacagca tcctcaaaaa
     4681 tctcatcgag aacttttcac agcatacttt gaacctgtcc gccgttcccg aggacgagct
     4741 tggcaacggt tacgagtacc tcattaagca attcgccgac gacagcgggc atacggcgca
     4801 ggagttttac accaacagga ccctcgttca tctcatgacg cagatgctcg aaccgaaagc
     4861 aggagagagc atctacgacc cgacggccgg cacaggcggc atgctcatct ctgccctggc
     4921 cgaggtcaaa agacagggtg gggacgttcg caccctgggc ctctacggac aggaagtgat
     4981 ccacatcacc gccgccatcg cccggatgaa ccttgtcttg cacggcgtgg aagatttcca
     5041 ggtcgtcgcc ggcaataccc tgagcaaccc cgctttcatc gagcgggacc gcctgcgcac
     5101 atttgatgtg gtcctggcca atccgcccta ttcaatcaag aggtggaacc gcgaggcatg
     5161 gacaagcgat ccatggggca gaaatttcct cggcacgccg ccgcagggac gggccgacta
     5221 tgctttcttc cagcacatcc tcaagagcct cgatcctaaa actggccggt gcgcgattct
     5281 cttcccccat ggcgtgctgt tccgcaatga agaagccgac atgcggcgca agcttgtcga
     5341 gttggacctg ctggaatgcg tccttgggct ggggccgaat ctcttttaca actcgcccat
     5401 ggaagcctgc gtcgtaatct gccggagctg caagccaccc gaccgcgaag gcaaagtatt
     5461 gttcatcgat gccgttcatg aagtggcgcg ggaacgggcg caaagttttc tcaagctgga
     5521 acatcagaag cgcattttgg ccgcctatca aacctttgcc gatgatcccg gttttgcgaa
     5581 ggtggcgacg aaaaaagaga ttctggacaa agacggcaat ctctcgattc cgcgctatgt
     5641 ccgacctgtg ggaaacggca acaacaatgg cgccggagat gttccaagtc tgaaaaaaat
     5701 gtgggcggcc tttgaagcca gtggtcgaga gttctgggag cagatggacg cgctggtgga
     5761 tatgctcgac ggcgttgtgg ccggagaggc ggaagatgcc tgagaagctg aaacaagggt
     5821 ggacaagggt tgcttttgga gatgtggtgc aattatgccg tgaacggtcc ggcgcccccc
     5881 ttgacgatgg ttttgatcgc tatgttggcc ttgaacacct cgaacccggc gatctgaaaa
     5941 ttcggcgctg gggcaatatc gcggatggca ccaccttcac caacgtgttc cgtaccggtc
     6001 aagttctttt tggtaaacgc cgtgcctatc agcgcaaagt tgctttagct gactttgagg
     6061 gtgtttgttc cggtgacatc tatgtgttca aaacaaaaaa tgaaaccctt ctgtctgaat
     6121 tactgccatt tatctgccag agcgatggat tctttaacca tgctattggc acctcggcgg
     6181 gatcgctatc gccgcgaaca aactgggaga gccttgcaag ttatgaattc gccctgccgc
     6241 cgccggagga gcaaaggctg attgcggaag tgcttttggc tcatttggag acaaggaaag
     6301 gttttcagaa cgcagttcgg tgtgcagaga ggctccggat gtcgctgcta cgatggcact
     6361 tttcagattg cggatcggag tgccttgtcg cccttcgcag tcttgtagac aacggcgatg
     6421 ttgagttgaa gaccggacca ttcggaactg tccttgcagc gaaggagtat caaacatcgg
     6481 ggtggcctat cgtgaaccct actgacatga agagcggccg gatctgtcat gaaggagggc
     6541 catgcgtgga tgacgcaacg gccaaccgcc ttgatgtcta tcggttacga gaaggcgaca
     6601 tcctactagc aaggaaagct gatctcagca aggctgttct tgctgcgacg gaacacactg
     6661 gttggattgg tggttcggac acaattcgtc ttcgctgcac ttcttcaaag gttcttccgg
     6721 gttatcttca tcttgcttta cagactgatg gcgcggcgcg gatgttatac tcctacgctc
     6781 atggaaccgt tatgccaggg ctgaatgaga aagcccttta tcgtatcagt atcaatctgc
     6841 cagatggccc agagcagcag gccgttgtcg atcactttcg tctgatcgag gctcgtgagc
     6901 gtgagttgct ggagcgtgaa gtggcctgtg cagttttagg ccgagttgcg tccgagcagc
     6961 ttttgacacc tgaaaggagt ctgatgtgac taacttcacc gaatccaaca ccgtcgaagc
     7021 ctacatccgt gaccggctgg ccggcccggt cagaatcaca cgtcctaatg agattcaggg
     7081 acaggtggca tcctttggga agccccgtct cggttggaat ttcgcatcgc cgatggatgt
     7141 gccgcgacag ccgcaggaag taatggtcga acgatgggtc cgagaagccc tcatccgctt
     7201 gaatccggag atcgccgcgc agcccgaccg ggcggatgat gtgctctaca agttgcgtgc
     7261 catcatcatg ggcgtgcgca gcgacggtct ggtcaagggc aacgaagaat ttgccgcctg
     7321 gctgaacggc gagcggtcga tgcccttcgg cccgaacggc gaacatgtca ctatccggct
     7381 gttggatttc agcgatttaa cgcagaacaa atatgtcgtc accacgcaat acaccattcg
     7441 cgccggggct acggaaaagc gcgccgacct cgttttgctg atcaacggca tccctctggt
     7501 ggtgattgag gccaagacgc cggtccgggc aagccagagc tggctggacg cggcgatcca
     7561 gattcatgag gattacgagc gcaacgtgcc ggagctgttt gtgccgaacc tgctttccat
     7621 tgccaccgag ggaaaggagt tccggtatgg ctccatcgga ctgccgctcg atttgtgggg
     7681 gccgtggcgt attgaatccg gcatcgcaac accggccctg caagaggtcg ccaagggagt
     7741 cgattctctc ctgcggccgc atgtccttct cgacttactg gccaatttta cctgctatgc
     7801 cacggacaag aagaaacacc ggatcaagat cgtcgcccgt taccagcaat tcgacggtgc
     7861 taacaagatc gtcgatcgcg ttgtcgcagg gtatccgaaa aagggtttga tctggcattt
     7921 ccagggatcg ggaaagtcgc tcctgatgct tttcgcggcg cgcaaactgc gtctgcatcc
     7981 ggcgcttaag aaccctaccg tcatgattat cgtggatcgc attgacctgg acactcaaat
     8041 atccagcacc ttttatgccg ccgacacccc caatctggtt aaggccgaca gccggacaga
     8101 actgcagcgg ctcctgggac aggatgtgcg gaaaatcatc atcacgacga ttttcaagtt
     8161 cggcgaggcg gacggcgttc ttaacgaccg gagcaacatc attgccatgg tggatgaggc
     8221 gcaccggacg caggaaggcg atcttggtcg caagatgcgt gatgcgctgc ccaacgcctt
     8281 tctgttcggt ttgaccggca caccgatcaa ccgggccgat cgcaacacct tctatgcctt
     8341 cggggcagat gaggattcag ctggttacat gagccgatac ggatttgagg agtctgtccg
     8401 ggacggagcg accaagccgc tgcattttga accacgcttg ctggaaatgc atatcgacaa
     8461 ggaagacatt gaccaagcat ttagggaaat gaccggaagt ttgagcgatc tggaccggga
     8521 tcaactggcc aagacagcag ctaagatggc cgtgctggta aagtcgccgg aacgtatcca
     8581 aaaaatttgc acggatattg cgcgacactt ccaggaaaat gttgcaccga acggattcgg
     8641 agcgcaggta gtcacatacg accgggaaag ctgtgtcctt tacaaaaagg cgctcgacga
     8701 gtatttgccg ccggaggtgt cggatatcgt aatgacggtc aatagcggtg agacggaata
     8761 cgccgcctac aaccgagatc gcgaatcgga agagaagttg ctcgaccggt tccgcgatcc
     8821 tgcggacccg ctgaaggtgc tgattgtgac ctcaaaactc ctgacgggat tcgatgcacc
     8881 aattcttcag gcgatgtacc tcgacaaacc gcttcgcgac catacgcttt tgcaggccat
     8941 atgccgcacc aaccggccct atggcgagac taagacgcac gggctgatag tcgattacct
     9001 gggcgttttc gatgatgtgg cccaggccat ccagtttgat gaggaaggga tcacccgtgt
     9061 tgtcaccaac atcaacgaac tgaaagccaa gctgccggag gccttgcaaa aatgcctgac
     9121 ctatttcgcg ggggtggacc gcacggttac aggctatgaa ggattgatcg cggctcagaa
     9181 ttgccttccc gacaacaaca cacgcgaccg attcgcggcg gactacagtt atctaagccg
     9241 attgtgggag gcgatttcgc ctgatccaat cctgagtccc catgaagccg actatcgctg
     9301 gctgtcgcag gtgtatgatt cggtaaaacc gtcaaccgga agcggccggt tattgtggca
     9361 tgcattaggc gccaagacta tcgaactgat ccaccagcat gtgcatatcg acgccatccg
     9421 cgatgatctg gaaacgctgg tcctggacgc ggacctgctg gaggccgttt tggggacacc
     9481 ggatccgaac aagaaagcca aggaaataga aatcaaagtg tcccgatggc tacggaaacg
     9541 tctccatgac ccacgtttca aagccttggg cgaacggctg gaggaactga aggtacggca
     9601 tgaacgaggc ctgctgacaa gtgttgagtt tctcaaagaa ctcctcaacc tggcccgtga
     9661 tgtcgtgacg gcggaacgtg cccagccgcc ggtagagcgt gaggtacaag ggaaagctgc
     9721 attgacaaaa ctctttgaag aggtgaaaaa tcctgagaca cccatcattg tcgcccgcgt
     9781 ggtgaacgac atcgacgaga tcgtgcgtta tgtccgattc gatggctggc aaaataccca
     9841 tgctggggaa cgggaagtta agaaagcttt gcggaagact cttttcaagt atcggctcca
     9901 ccaggaccag gaactattta atagggccta tgggtatatc aggcagtatt actgaggccc
     9961 tatgaaaaca tagctgcgtt ttcatggggc ccatttcgag ttgtattcta ccagtttccg
    10021 ctaaggtcta acgaaactat caccggccgg cggaacgccg gtccgcgtgg attgtatggt
    10081 tgggatagat tttatgattc atattggatc tgcgggagcg agcgtacaga aacctacggt
    10141 tatgatgtaa cctcccgttc cgcttcaata tatttataga gtgttggttt ggaaataccc
    10201 atcatctggc aaatctggga aaccgtgtgc tccttcttat cgtatagctt caccgccagt
    10261 attcttttgt taagatccag cgcccttggc cttccccctc ttctgccccg tgcccttgca
    10321 gcggtaagtc ccgcctgagt tcgctctcgg atcaggttac gctcgaattc agctagggct
    10381 ccaaagatgt gaaagatcag ttttcccgag ctcgacgacg tgtcgatagc ttcatgcaaa
    10441 cttttcagac tgatgccctt cgattcgaga agtgtgacca tttcgatgag gtccttaagt
    10501 gaccggctga ggcggtccaa acgccacacc acaagaacat caccatccct tgcaatatct
    10561 aacgctaatt tcaatccagg acgctcagct tttgcgccac ttaaccgatc ttccgtaata
    10621 cgttcgcacc cagcctgggt gagtgcatcc ctctggagat ccagattttg atcatcagta
    10681 gaaactcgtg catagccaat tagcatgaac agcctcagga aattgaagtt aatttaccca
    10741 ttatatgatg aactaatttt acattgattt atttaccaag ataatttacc atttgcaagg
    10801 gcaaattggg tggtttttca actttcccct atcggtaaag aaaacctccg ttttatttac
    10861 tga
//
LOCUS       DAOSMF010000068        10576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_7146_length_10576_cov_18.585876, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000068 DAOSMF010000000
VERSION     DAOSMF010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 10576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10576
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7146_length_10576_cov_18.585876"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..935
                     /locus_tag="PLT63_07470"
     CDS             <1..935
                     /locus_tag="PLT63_07470"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HOD98600.1"
                     /translation="GEAKTVFRLASMTPMEHTYNQGAVRFAQLVRERSRGRIEIAVYP
                     NGELAKGERELLEAIQEGSIDFYVGSTGPLSGFSPSMGILDLPFLFRDYSHADQVLDG
                     SVGNRLLTDLEKANLKGLAFWENGFRNLTNSKRAVKTPGDARGLKIRTMENKLHLAAW
                     KAVGVQPIPMAYGELYGALQRKEIDGQENPVAVIYTGKFNEVQRYLSLTQHVYSPALL
                     IVSPKTWLNLGKEDQDMLIKTAREVAPYQRRLGRESEEKQIAALAAAGMTVSKDIDKA
                     AWYRVMEAAMGDFIRSFGKDQVAAIINAGEGGAR"
     gene            1055..2689
                     /locus_tag="PLT63_07475"
     CDS             1055..2689
                     /locus_tag="PLT63_07475"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOD98601.1"
                     /translation="MTFNTSIVHSRSKKIFLTFFALASLLPILIAIFIMRQYVLPFLS
                     AEDAERLRFILQLGASTMLFFPLLSFFLMFRWLNSLENVTTEIVSKTQAVASREEEFA
                     GQKIDKTNDYADPVAPPQPEENEIQSLIRSFNTIFQTAADQLAERNHIKDLLARLIGV
                     SANLTSELDFDRLFPLIIGNVTQAMEAERTSLYVVDWDRRELWTMVAEGVQQIRLPLG
                     QGISGRVAATGEIINVPDAWELPYFDRSFDERNNFRTRSVLCQPIRGRFGKTIGVVQV
                     INKQGKDRFEEEDETFLKGLVSQVGIALENSLLVDEMKLSFEASIKTLSATVDSRHPM
                     TAGHSERVTEYAQMIAKVMKLGKEEQEAIKYAAILHDIGKIGIRDEILLKEGRFTPED
                     WEEMKTHPRKTKTILEQFHFPRHLRNVPEIACRHHERIDGTGYTDGLTGDQIPLGAKI
                     IAVADVFDALTSKRDYPKYTPEDTLDAAPIPLSEVIELMKSQAGSHFDLRVMNAFLNC
                     LPEALLRHRGDHFPTAYVDDVLKTLQTPSQPAAASH"
     gene            complement(2683..3465)
                     /locus_tag="PLT63_07480"
     CDS             complement(2683..3465)
                     /locus_tag="PLT63_07480"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HOD98602.1"
                     /translation="MLVVGNDIVDLTDPYNQGKLRDRRFRGRVFTAAEERRIDASPRP
                     DVTLWAFWAAKESAFKAAVKLKPGSGFIPKEYMVVKYEMDLPAAGLSSLPDDYPIMGL
                     DGEKGTGEDRKQEQTVVLYSGETVAASPRKSGLIATPVGSMPLEFTISEQWIHCLALG
                     RRGCEDTATPDRTIHVVMESRGMCIEPSAAVRRAAVHHLADIFRTSPYYLEIRRARTS
                     YGYGGPPQVFLAGRQTDVDISLTHDGSYIAFALVTPETSTDQ"
     gene            complement(3350..4069)
                     /locus_tag="PLT63_07485"
     CDS             complement(3350..4069)
                     /locus_tag="PLT63_07485"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOD98603.1"
                     /translation="MDATRRHWAELRRRHPGPWLICGNHLTLIDSMLLTYGLMSLSSH
                     FKEFRALPWNLPERANFQKQLWKGLLCYLAKCIPVSRGGDREEMQTTLGKCNWVLQNN
                     RVLMIFPEGGRSRAGRIDQENFSYGIGRFLQDHPSCRVLCVYLRGDRQDAYSDLPARG
                     ESFTITIDVFEPRRPVKEGLRGQRELAGQIVSHLAQMEEEYFAGRRQRHCGFDRSVQP
                     GKASGPALPRPGLHGSGRTAD"
     gene            complement(4259..4507)
                     /locus_tag="PLT63_07490"
     CDS             complement(4259..4507)
                     /locus_tag="PLT63_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HOD98604.1"
                     /translation="MEEKEIFKEMIDILRIYAKDKAGLDTATMATHILDDLKVNSARL
                     VDVIIKCEDVFGITIEDDEADKIRTIGDAVNLIKNKLG"
     assembly_gap    4553..4652
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4654..5934)
                     /locus_tag="PLT63_07495"
     CDS             complement(4654..5934)
                     /locus_tag="PLT63_07495"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HOD98605.1"
                     /translation="MNKRVVVTGMGVTAPNAHGLDDFTTALRAGRSGIRHIKELEALN
                     FACQVGGIPENFDAVRRSYFDHEKLMSINDNIGYASVSAIDAWRDAGFEVPEGDGEDV
                     DWDTGVIIGSGIGGMDTIARTVVPMVNDGKVRRMGSRIVEQVMNSGTSARIAGLLALG
                     NRVTSNSSACSTGNEAIIEAAWRIRAGLAKRMLAGGSEGASPYIWSGFDAMRVICRKF
                     NDDPERASRPLSASAAGFVPGAGGAVLVLEDLETAQRRGARIYAEVAGGAVNCGGQRR
                     GGSMTAPNPEGVRRCIHQAMADAAVSPHEIQAINGHLTATYADPVEVKNWAAALNRTP
                     ADFPFINSTKSMIGHCLGAAGAIESVAVILQLYGGFLHPSVNSEDVHPEIEAFGDSIP
                     QQALDMPDLRVIAKAGFGFGDVNSCLIFRSWEGA"
     gene            complement(6041..6526)
                     /locus_tag="PLT63_07500"
     CDS             complement(6041..6526)
                     /locus_tag="PLT63_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hydroxyacyl-ACP dehydratase"
                     /protein_id="HOD98606.1"
                     /translation="MAADQTMRETILAAVPQQPPFRFIEEIRELDEAGIVGSYRFRRE
                     EYFYRGHFPGQPVTPGVILIEAMAQTGVVAQGLYLSLLKSGAALRDLRKITTLFTLAE
                     EVEFLDIVAPGEEIVFRGRTIYFRRGSLKSAVTGERKNGQIVCRGILAGAGIDRSAGC
                     E"
     gene            complement(6516..7244)
                     /locus_tag="PLT63_07505"
     CDS             complement(6516..7244)
                     /locus_tag="PLT63_07505"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOD98607.1"
                     /translation="MEDKRVALVTGGGTGIGAACVRSLAATGFLVGIHYHRSEEGARA
                     VLAETGEGLLLPGDLTRSEDIDALVEQLKSATGRLDVLVHNAGVSLNADINAMRLEQF
                     DAQRLLARGVWYLTKRVLRQFMLRRNSGRIITISSVVGHTGNGGQIPYTMEKAALDAF
                     TKSLAKELSGRNILVNAVAPGFIDTAMTASLPEVARERIMANIPLQRMGRPEEVAEVV
                     TFLATAGTYITGSVIHVNGGLYGG"
     gene            complement(7354..7914)
                     /gene="fabA"
                     /locus_tag="PLT63_07510"
     CDS             complement(7354..7914)
                     /gene="fabA"
                     /locus_tag="PLT63_07510"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HOD98608.1"
                     /translation="MEKSDFDLPELIAFSYGKLVTDPPAGFDARLPAPPFLMIDRILI
                     LEADGKRGRIVAEKDIQIDAWYFQCHMPGDPVQPGCLGVDAIWQLQGFYGVWRGGLGA
                     GRALGCEGVNFTGQVRPYHRVVRYEIAIRRFTALAESGASVIIGDGAISVDGELIAEV
                     HQARTGLFRGIVYGDYPWLPPATMQT"
     assembly_gap    7970..8069
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8077..9201)
                     /locus_tag="PLT63_07515"
     CDS             complement(8077..9201)
                     /locus_tag="PLT63_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HOD98609.1"
                     /translation="MLYLHGLGHFHPENVITNRFLADLDIGTSDEWVLERVGIRERRT
                     SLELDYIRETKNQDPRGASEASRYNNAETGAAAARQAMRQAGIAAADVGLVISGSSAP
                     DHVTPSEAATVAAELGIEAPCLDINAACATFGVQLSFLDRMRPETLPSFILVVNPENL
                     TRCVDYADRNTAVLFGDGSAAAVVSAKEPARLRLMDCHCASQPSAWQKAVIPRMGYFH
                     QEGNAVQGFAIRKMTEAVRIHRERWKSGLSSPNACRSGLKGAADRVEKRVDADSGSGE
                     TMVLTAEGEVAAGGNGRRLVFIGHQANLSVLNTVCERSAISDDCHWRNVALFGNTGSS
                     GAPAVMSQRQKDLRPGDVLIMALVGAGLTWATLVWQAGEC"
     gene            complement(9204..10403)
                     /locus_tag="PLT63_07520"
     CDS             complement(9204..10403)
                     /locus_tag="PLT63_07520"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOD98610.1"
                     /translation="MDLNLSATQGMYVETVRRFVKTEILPGLLEVERKHAFPFSVVRR
                     AWELGILNLSIPAVIKGFELDALSSALIIEELAYGDSGIATSAMCNDLANAVIAQHGI
                     PSQQEAFLRPFVEEPLLASFCLTEPGAGSDNSAMTTFMVRREDGSYLLNGTKCFITNA
                     SFASQFTVFCKVGKPAGNFMACVIVPVPPPALEAIAGGFPTGREIFLPAGGCIAVGKP
                     EDKLGQRLSNTAAVTFEEVVVAPEQIIGDRRRGFQYVIDVLDYARPMVAAIGVGLARR
                     ALAVTIDYTRERRQFGQRICDLPVARETLTAMWKKVELAKLALIKAAVTLDEKGSDRG
                     LYASLAKNAAAEAAIFCANEGLHLHGGYGFMNEYEIAKLARDAHIIDIYEGVREVQNM
                     IIGREIC"
BASE COUNT         2183 a         3237 c         2882 g         2074 t
ORIGIN      
        1 aaggtgaagc gaagaccgtc ttcaggcttg cctcgatgac gcccatggaa catacctaca
       61 atcagggggc ggtccggttc gcccaactgg tcagggaaag gagccggggg cgcatcgaga
      121 ttgccgttta tcccaatgga gagttggcca agggggaacg ggaattactg gaagccattc
      181 aggagggaag tatagacttt tacgtaggtt ccacaggccc cctcagcggc ttcagtccgt
      241 ccatgggcat cctggatctg ccctttctgt tccgtgacta cagccacgcg gaccaggtac
      301 tggacggttc ggtcggcaac aggctcctga cggatctcga gaaggccaat ctcaagggtc
      361 tggccttctg ggaaaacggc ttccgcaacc tgaccaactc gaaaagggcc gtaaaaacac
      421 cgggcgatgc cagggggctg aagatccgca ccatggaaaa caagctgcat ttggccgcct
      481 ggaaggccgt cggcgttcag cccataccga tggcctatgg cgaactctac ggcgccctgc
      541 aacgaaagga aatcgacggc caggaaaatc ccgtcgccgt catctacacc ggcaaattca
      601 acgaggtaca gcgctacctc tccctgaccc agcatgtcta ttccccggcc cttctgatcg
      661 tcagccctaa aacctggctg aaccttggca aggaagacca ggatatgctc ataaaaaccg
      721 cccgggaagt ggccccctat cagcggcgat tgggaaggga aagcgaggag aagcagatcg
      781 cggctctggc cgccgccggc atgacggtaa gcaaagatat cgacaaggct gcctggtacc
      841 gggtcatgga agcggccatg ggagatttta tcaggtcatt cggcaaggat caggtggcgg
      901 cgatcatcaa tgccggtgaa ggcggagcaa gataagctcc accggcaact tcgcactccc
      961 gatccgtttc cgatcccggg atgaaatccc accgggatgg ttgccgtaaa gaagtcccgg
     1021 tccagatcat caatgcatcg ggagttgtga acttatgacc tttaacacat ccatcgtcca
     1081 ttcccgttcc aagaagatat ttctaacatt ttttgctttg gcatccctgc tccccattct
     1141 catcgccatt ttcatcatgc gccagtacgt gcttcccttc ctgtccgccg aagatgcgga
     1201 gcgtttgagg tttatcctcc aactcggcgc gagtaccatg ctatttttcc cgctgttgag
     1261 cttttttctc atgttccggt ggcttaattc cctcgagaat gtcacgacgg aaatcgtctc
     1321 caagacccag gccgtggcca gcagggaaga ggagttcgca ggccagaaga tcgacaaaac
     1381 caatgactat gccgaccccg tggccccgcc gcagccggag gaaaatgaaa tccagagtct
     1441 catccgttct ttcaatacca tcttccagac cgcggccgac cagcttgccg aacgcaacca
     1501 catcaaggat ctgctcgccc gattgatcgg ggtgtccgcc aaccttacct cggagttgga
     1561 tttcgaccgg ctgtttcccc tcatcatcgg caacgtgacc caggccatgg aggcggaaag
     1621 gaccagcctc tacgtcgtgg attgggaccg ccgggagctt tggacgatgg tcgccgaagg
     1681 ggtgcaacag atccggctgc cgctggggca gggcatcagt ggccgggtcg cggcaacggg
     1741 agaaatcatc aacgtgccgg atgcatggga attgccctat tttgaccgct ccttcgatga
     1801 gagaaataat ttccggaccc gttccgtcct ttgccagcca atccgggggc gattcggcaa
     1861 gacgatcggc gtggtgcagg taatcaacaa acagggaaag gatcgtttcg aggaggagga
     1921 tgaaactttc ctgaagggat tggtttccca ggtgggcatc gccctggaaa actccctgct
     1981 ggttgatgag atgaaacttt ctttcgaggc ctcgatcaaa accctctccg ccaccgtcga
     2041 ttcccgccat ccaatgaccg cgggacattc cgaacgggtc acggaatacg cccagatgat
     2101 cgccaaggtg atgaagctgg gcaaggaaga gcaggaggcc atcaaatacg ccgccatcct
     2161 tcacgatatc ggcaagatcg ggatccggga cgagatcctg ctgaaggagg gacggtttac
     2221 cccggaggat tgggaggaaa tgaagaccca tccccggaag accaagacca tcctggagca
     2281 gttccatttc ccccggcacc tgcgcaacgt tccggaaatc gcctgccggc accacgaacg
     2341 aatcgacggc accggctaca ccgacggcct cacgggcgat cagatacccc tcggggccaa
     2401 gattatcgcc gtcgccgacg tcttcgacgc cctgacatca aagcgcgact atcccaaata
     2461 caccccggaa gataccctcg acgccgcccc gataccgctt tccgaggtga tcgaactgat
     2521 gaaatcccag gcggggagtc atttcgatct tcgggtaatg aatgccttcc tgaattgtct
     2581 tcccgaagcc ctgctacgcc accgggggga tcactttccc accgcctatg tggacgatgt
     2641 actcaagacg ctgcaaacgc cttctcagcc cgcggcggcg tctcattgat ccgttgacgt
     2701 ttcaggtgta acgagggcaa aagcaatata ggagccgtca tgggttaggc tgatatcgac
     2761 gtcggtctgc cggccagcga gaaatacctg gggcggcccc ccataaccat aagaagtcct
     2821 ggcccggcgg atttccaggt aatacgggga agtccggaaa atatcggcca gatgatgaac
     2881 tgcggcccgc cgtaccgcgg cggacggttc aatgcacatc ccgcgacttt ccatcacaac
     2941 atgaatcgtc ctgtcagggg tggcggtatc ttcacatccc cggcgcccca acgcaagaca
     3001 gtgaatccat tgttcggaaa ttgtgaattc caaaggcatg gatcccaccg gagtggcgat
     3061 caaaccggat ttacgagggg aagcagccac tgtctctccc gagtacaaaa ccacggtctg
     3121 ctcctgcttt cgatcctccc ccgttccttt ttcgccatcc agccccatga tgggataatc
     3181 gtcgggaagc gaagacagcc ccgccgcggg gagatccatc tcatacttga caaccatgta
     3241 ttcttttggg atgaatcccg atcccggttt cagctttacc gccgccttga atgccgactc
     3301 tttggcggcc cagaacgccc agagcgttac atcgggacgc ggtgaagcat caatccgccg
     3361 ttcttccgct gccgtgaaga cccggccgcg gaagcgccgg tcccgaagct ttccctggtt
     3421 gtacggatcg gtcaaatcca caatgtcgtt gccgacgacc agcaaaatat tcctcctcca
     3481 tttgcgccag gtgggaaaca atctgtcccg ccagctcccg ctgtcctcga agcccctcct
     3541 ttaccggtcg ccggggttca aagacatcga tggtgatggt aaatgactcc cccctggccg
     3601 gaagatcact gtaagcatcc tgacgatctc cccggagata gacacagagc acccggcatg
     3661 acgggtggtc ctggagaaag cgtccgatgc cgtaagagaa attctcctga tcgatcctgc
     3721 ccgcacggga acgccccccc tccgggaaga tcatcaacac ccgattattt tgcagaaccc
     3781 agttgcattt ccccagggtt gtttgcatct cctccctgtc cccgccccgg ctgaccggaa
     3841 tgcacttggc cagatagcag agcagccctt tccagagctg tttctggaaa ttggcccttt
     3901 cggggaggtt ccagggaagg gcgcgaaatt ctttgaaatg agagctcagg gacatgaggc
     3961 cataggtcag gagcatggaa tcgatgaggg tcaggtgatt tccacagatc agccaggggc
     4021 cggggtgtct gcgccgcaac tccgcccagt gacgacgtgt ggcatccaaa tcccggagac
     4081 ggtatccccg gcatcgtaaa aaaagaaaat agagggggcc gacgaccgct acagtgagca
     4141 atcccatggc ctcctgccgc catagattat aatgaactcc agccaccgta ctcatcattc
     4201 agcttgcagc attgctatcg tctcttatca tccaatcaac ggcaccatcg tacccccatt
     4261 atccgagctt gttcttgatg agattcacgg catcgccgat agtgcgaatc ttgtcggcct
     4321 cgtcgtcttc gatggtgatc ccgaagacgt cttcgcattt gatgatgaca tccaccagac
     4381 gtgccgaatt caccttaagg tcatcgagga tgtgggtggc catggtcgcc gtatccaaac
     4441 ctgctttgtc cttggcatag atcctcaaga tatcgatcat ttccttgaat atttccttct
     4501 cttccatatt cctctcctta cattttttat gatttattcc cggcagcggc tannnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnatcatgcc ccctcccagc tcctgaagat
     4681 caggcaactg ttcacatcgc cgaaaccgaa gcccgccttg gcgatgacgc gtaaatccgg
     4741 catgtccagc gcctgctggg ggatgctgtc cccaaaggcc tcgatctcgg gatggacatc
     4801 ctcactgttg accgaaggat ggagaaagcc gccgtagagc tgcaatatca cggcgactga
     4861 ctcgatagcc cccgccgccc ccagacaatg gccgatcatg gatttggtgg aatttatgaa
     4921 cggaaaatcc gccggggttc gatttagggc cgcggcccag ttcttcacct ccacgggatc
     4981 ggcataggta gccgtcaggt ggccgttgat cgcctgaatc tcgtggggcg aaacagcggc
     5041 gtcggccatg gcctgatgga tgcaccggcg gaccccctcc ggattggggg ccgtcatgga
     5101 accgccccgg cgctggccgc cgcagttcac cgccccgccg gccacctcgg catagatccg
     5161 cgccccccgc ctttgggctg tctccaggtc ctccaggacc agaacggcgc ccccggcgcc
     5221 gggaacaaaa ccggcggcac tggcgctcag ggggcgggag gcccgctccg gatcgtcatt
     5281 gaacttccgg cagatcaccc gcatggcgtc gaagccggac caaatatagg gcgaggcgcc
     5341 ttcggagccc ccggcgagca tccgcttcgc cagacccgcc cggatccgcc aagccgcttc
     5401 gatgatcgcc tcgttgcccg tggagcaggc cgaggagttg gatgtcaccc ggttgcccag
     5461 ggccaggagg ccggcgatgc gggcgctggt tccgctgttc atcacctgtt ccacaatccg
     5521 gctgcccatg cggcggactt tgccgtcatt gaccatgggc actaccgtcc gggcgatggt
     5581 gtccatcccg ccgatgccgg aaccgataat caccccggta tcccaatcca cgtcctcacc
     5641 gtccccttcg ggaacctcaa aaccggcgtc acgccaggca tcgatggcgg atacggaggc
     5701 gtatccgata ttgtcgttga tggacatgag cttttcgtga tcgaaatagc tccggcgcac
     5761 cgcatcgaaa ttttccggaa tgccgcctac ctgacaagcg aagttgagcg cctccagctc
     5821 cttgatatgg cggatccccg aacgtcccgc tcttagggcg gttgtaaagt cgtccaatcc
     5881 gtgggcattc ggggcggtca ctcccatacc ggtaaccacc actctcttgt tcataaccaa
     5941 aatacgctcc tatctgcttt tcgcaattcg caagggatac ggtctcttac catcccgatc
     6001 atcacccgtc cggaacatgc ggcctctatt tacttaccct tcattcacaa ccggctgacc
     6061 tatcaatccc cgcccccgcc aagatccccc ggcatacaat ttgcccgttt ttccgctctc
     6121 ccgttacggc ggatttcagg ctgccccggc ggaaatagat cgtacggccc cgaaagacaa
     6181 tttcctctcc gggggccacg atatccagga actccacttc ttcggccagg gtgaacaggg
     6241 tggtgatctt cctcaagtcc ctgagggccg ccccggactt gagcagcgaa agatagaggc
     6301 cctgagccac cacccccgtc tgggccatgg cctcgataag gatcaccccg ggggtcaccg
     6361 gctggccggg gaagtgtccc cggtagaaat actcttcccg gcggaaacga taggagccca
     6421 cgatccccgc ttcgtccagt tcccggatct cctcgatgaa gcgaaagggt ggctgctggg
     6481 gcaccgccgc caggattgtc tcccgcatcg tctgatcagc cgccatagag gcctccgttg
     6541 acatggatga ccgaaccggt gatgtaggtg ccggccgtgg cgagaaaggt cacgacctcc
     6601 gccacctctt ccgggcgtcc catgcgctgc agggggatat tggccatgat ccgctcccga
     6661 gccacctcag gcagggaagc cgtcatcgcc gtatcgatga agccgggggc cacggcattg
     6721 acaaggatat tacgaccgga aagctctttg gccagggatt tggtaaaggc atccagggcg
     6781 gctttttcca tggtgtaggg gatctggccg ccgttaccgg tatggccaac gacactggat
     6841 atagtgatga tccttccgga attccttcgg agcatgaact gacgtaggac ccgcttcgtc
     6901 aaataccaaa cccctctggc aaggaggcgc tgggcatcga actgttccag acgcatagcg
     6961 ttgatgtcgg cgttgaggga aacaccggca ttatggacca ggacgtccag acgtccggtg
     7021 gctgacttta gttgttccac cagggcgtct atatcctcgc tgcgggtcag atcccccggc
     7081 aggagcagac cttcccccgt ctcggccagc acggcccgcg ccccttcttc actccggtgg
     7141 taatggatgc ctaccagaaa tcctgtcgcc gccagggagc ggacacaggc ggcgccgatt
     7201 cccgtgcctc ccccggttac gagggcgact cttttatctt ccattgcttc tctgcccttt
     7261 cattccggtc gaaatcgaca accggttatc catgttctcc agagatcgcc aatgcatatt
     7321 ctctcatccc atccgccgga aaaggcatgt tcctcacgtc tgcatcgtgg ccggaggcag
     7381 ccagggatag tcgccataga caataccacg gaacaggccg gtccgggcct gatggacctc
     7441 ggcaataagc tcgccgtcta ccgaaatggc gccgtcgccg atgatgacgc tggccccgga
     7501 ttcagcaagg gcggtaaatc tccggatcgc tatctcgtaa cggacgacac ggtgataagg
     7561 ccgcacctgt cccgtgaaat tcacgccctc gcagcccagg gcccgtccgg cccccaggcc
     7621 gccccgccag accccgtaga agccctggag ctgccagatg gcatccactc ccagacatcc
     7681 cggctggacc ggatcgccgg gcatgtggca ctggaaatac caggcgtcga tctggatatc
     7741 tttctccgcc acgattcttc cccgctttcc gtccgcctcc agaatcagga tgcggtcgat
     7801 catcaggaat ggcggggccg gcaggcgggc atcgaaaccg gcggggggat cggtcacgag
     7861 ctttccatac gaaaatgcga tcaactcggg tagatcgaag tcacttttct ccataaactt
     7921 ctgataaggc aacatcatcc tgctctgtcc tcaatgcctg tggccacttn nnnnnnnnnn
     7981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng aagatttcaa cactcgccgg cctgccagac
     8101 cagcgtcgcc cacgtcaggc ccgcgccgac cagcgccatg atcagcacat cccccggccg
     8161 gagatccttt tgacgctggc tcataacggc gggggcgccg gaacttcccg tgttgccgaa
     8221 aagggccaca ttacgccagt ggcagtcatc ggatatcgct gaacgttcac agacggtatt
     8281 cagcacactg aggttcgcct gatggccgat aaagaccaga cgtcggccgt tgccgccggc
     8341 ggcaacttct ccctcagccg tcagcaccat ggtctcaccc gacccgctat ccgcatcgac
     8401 ccgcttttcc acccggtctg ccgccccttt caaacctgaa cggcatgcgt ttggtgaaga
     8461 caggccactc ttccagcgct cccgatggat ccggacggct tcggtcatct tgcggatggc
     8521 gaaaccctgg acggcatttc cttcctggtg aaaatatccc atccggggaa tgactgcctt
     8581 ctgccaggct gacggttgcg aggcgcagtg gcagtccatg agccgcaacc ttgccggttc
     8641 ctttgccgaa acaaccgcgg cggcgctccc atccccgaaa aggacggctg tattgcgatc
     8701 cgcataatcg acacagcgcg tgagattctc cgggttgacg acgaggatga aggatggcag
     8761 agtttccggc ctcatccgat ccagaaaaga caattggacg ccaaaggtgg cgcaggcggc
     8821 gttgatatcc aggcagggcg cctcgatacc cagttccgcc gccacggttg ctgcctcgga
     8881 gggggtgaca tggtcgggag cggagctgcc ggaaatgacc aaaccgacgt ccgccgcggc
     8941 aatccccgcc tggcgcatgg cctgacgggc cgccgccgct ccggtctcgg cattgttgta
     9001 acggcttgcc tcggaggccc cccggggatc ctgattcttt gtctcccgaa tgtagtcaag
     9061 ctccagggag gtgcgccgct cccggatccc cacccgctcc agaacccatt cgtcactcgt
     9121 cccgatgtcc aggtccgcca ggaagcggtt ggtgatgacg ttttccggat gaaaatgtcc
     9181 caaaccgtgg agataaagca tggtcaacag atctcccgac cgatgatcat gttctgcacc
     9241 tcccgcaccc cctcgtatat atcgatgata tgggcatccc gggccagctt ggctatctca
     9301 tattcgttca tgaatccgta gccgccatgg agatggagcc cttcattggc gcagaaaatg
     9361 gccgcctcgg cggcggcgtt cttggccagg gaggcgtata acccccggtc cgatcctttc
     9421 tcatccaggg tgacagcggc ctttatgagg gcgagcttag ccagttccac ctttttccac
     9481 atcgccgtca gcgtttcccg ggccaccggg aggtcgcaga ttcgctggcc gaactgccga
     9541 cgttcccggg tatagtcgat ggtcaccgcc agggcccggc gggccagacc gactccgatg
     9601 gccgccacca tggggcgggc ataatcgagg acgtcaatga cgtactgaaa tccccggcgc
     9661 cggtcgccga tgatctgttc cggggcaaca accacttctt caaaggtgac cgccgcggta
     9721 ttggataagc gctgtcccag cttgtcctcg ggcttgccca cagcgataca ccccccggcc
     9781 ggcaggaaga tttccctccc cgtcggaaag cccccggcaa tagcttccag cgcgggaggt
     9841 ggaacgggaa cgatgacaca agccatgaaa ttccccgctg gcttgcctac cttacagaaa
     9901 acggtgaact gggaggcgaa ggacgcgttg gttatgaagc acttggtgcc gttgagcaga
     9961 tatgatccat cctcccgccg gaccatgaac gtggtcatgg cgctgttgtc cgaacctgcc
    10021 cccggttccg ttagacaaaa ggatgccagc aggggttctt ccacaaaggg acgcaaaaag
    10081 gcctcctgct gggaaggtat gccgtgttgg gcgatgacgg cattggccag gtcattgcac
    10141 atggcggagg tggcgatccc ggaatctcca taagcgagtt cctcgatgat cagggcggag
    10201 gacagggcat ccaactcgaa gcctttgatc actgccggga tgctcaggtt gaggattccc
    10261 agttcccagg cccggcgaac tacggagaag gggaaagcgt gctttctctc aacctccagg
    10321 agaccgggaa ggatctccgt tttcacgaat ctccgcaccg tttctacata catgccttgc
    10381 gttgccgaca aatttaaatc catggctcag ttgctcccga aaatccgcgc cgctccggaa
    10441 agggtgacca cggagaggca gttgaccccc agggtcttga agaaatctcg caatggccga
    10501 cccgggccta tctcatagat tgtgtcggct tcggcggcga tgagttccat gttctcccgc
    10561 cagcggaccc ggcttc
//
LOCUS       DAOSMF010000069         9941 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_7730_length_9941_cov_25.448108, whole genome shotgun sequence.
ACCESSION   DAOSMF010000069 DAOSMF010000000
VERSION     DAOSMF010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 9941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9941
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7730_length_9941_cov_25.448108"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..846)
                     /gene="pyrF"
                     /locus_tag="PLT63_07525"
     CDS             complement(82..846)
                     /gene="pyrF"
                     /locus_tag="PLT63_07525"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOD98611.1"
                     /translation="MTNIRSDLPRERLIFALDIGRGGIDEALAWVDRLQGHVGLFKVG
                     KEAYTIYGPTLVERIQERGARVFLDLKYHDIPNTVARAAAGAVGLGAAMFNVHAAGGR
                     RMMAAAAAAAQDEAARLSKPLPLLIAVTVLTSLDDDDLREIGFRIGSLDAVRNFACLA
                     RESGLAGVVASPREIGAIRAVCGEDFVIVTPGIRGTDPIAGDDQKRTLAPETAIAAGA
                     DYLVVGRPIRMAVDPAAEADRIVAAIAAGLKARPGS"
     gene            complement(827..1099)
                     /gene="rpoZ"
                     /locus_tag="PLT63_07530"
     CDS             complement(827..1099)
                     /gene="rpoZ"
                     /locus_tag="PLT63_07530"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOD98612.1"
                     /translation="MARITVEDSLRQAKNRFALVLLTSQRAIQLLKGSRPLEEGRNNR
                     EIVTALREIAVGKVMYAHPENLKYTKENFRPIIDQTEFTEDDEYTE"
     gene            complement(1282..1890)
                     /gene="gmk"
                     /locus_tag="PLT63_07535"
     CDS             complement(1282..1890)
                     /gene="gmk"
                     /locus_tag="PLT63_07535"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOD98613.1"
                     /translation="MSNNSVIMVVSAPSGTGKTSICRPLLREFPALRFSVSFTTRPPR
                     RGEADGRDYHFVTPDAFREKIQAGDFVEWAENYGHLYGTSRGVIEEIRGRGEDILLDV
                     EPRGARALKEIYPKGVFVFVLPPDLAELQRRLWKRGDGDEEVRRWRYQKARDEIQEVF
                     WYDYIVVNDRLEQAIERMRAIYLAEKCRRERQMGIVSPFLAE"
     gene            complement(1880..2764)
                     /locus_tag="PLT63_07540"
     CDS             complement(1880..2764)
                     /locus_tag="PLT63_07540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOD98614.1"
                     /translation="MIRSMTGFGREEGAAAGKRIQVEMKSLNHRYLEISLRVPAALAA
                     LEAEIKKKIGERFSRGKIEVTIRLESDGHQEEAPALTLNLPLVRQYHALLTRIKTELN
                     LPGEVTLEMMTGFRDLYVPGEAEENLSALWAGLERVLLQAMDRLAAMRKAEGEALGRD
                     LATRMEMISGLLEDITRRSPAVVQEYRQRLAERVRELTQGMVIDEARLHQEVAILAEK
                     SDINEEVVRLESHINQFGEFLTAGEAVGRKLDFLIQEMNREINTIGSKSQDGEIARRV
                     IEIKSELAKLREQIQNVE"
     gene            complement(2757..3302)
                     /locus_tag="PLT63_07545"
     CDS             complement(2757..3302)
                     /locus_tag="PLT63_07545"
                     /inference="COORDINATES: protein motif:HMM:NF025743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HOD98615.1"
                     /translation="MSIPRPAPPVNLFVSVIGTEGEVIAAAIALLMERFGEADYISPC
                     REFAYTDYYEREMGRGLRRRFVSFAGHISPESLPEIKLWTNDLERRLIAGETRRVNID
                     PGYVAAAHLILATGKGYDHRPYLRSGIYADLTLVYRAGAFRPLPWTYPDYAAPETLAI
                     IQSVRRKYLGQLQKIRKENHD"
     gene            complement(3299..4288)
                     /gene="rfaE1"
                     /locus_tag="PLT63_07550"
     CDS             complement(3299..4288)
                     /gene="rfaE1"
                     /locus_tag="PLT63_07550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HOD98616.1"
                     /translation="MRRLFTKKRALEVMDQFTQAKVLVVGDIMVDHFIRGQVARISPE
                     APVPVVDVRSDHLLWGGAANVMNNIHAMGGRVYGVGVVGGDDTGRRLLAELAAKDIAT
                     MGIFVEDRRPTTLKTRIVAQGQQVVRFDRESRVPVSEEAIAGIIDYMESLRGDLGAVV
                     ISDYSKGVVTPSLIEGVRRIFAGSTVVVCVDPKKNDFSLYRDFDIVTPNHHEASRAVG
                     FEITDRSSLVKAGRELIRKYGFRALLVTRGEEGMSLFERDGTIRHTEFPAEAREVFDV
                     TGAGDTAIGVFALAMASGATFREAAFMANRAAGIAVGKVGTATVTRKELKRTL"
     gene            4477..5199
                     /locus_tag="PLT63_07555"
     CDS             4477..5199
                     /locus_tag="PLT63_07555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOD98617.1"
                     /translation="MFRSILLVLISVIITAFFSTCVVVFSLLGAAENSLHKVARTWAK
                     VLLAISNVQVRVIGTEYVDGETPQIFMSNHQSDFDILIILAFLPGQFRWIAKKELFRI
                     PVFGRAMKNAGYIEIDRQHHQRALQNLAEAARKIREGKSVMSFPEGTRSKDGSIKPFK
                     KGMFHLAMEAGVPIVPITIIGADEIMPKRSLKVNPGRITMIIDAPIDIDEYTDDARTE
                     LMERVRGIIVKNFDMWREWKDS"
     gene            5184..7355
                     /locus_tag="PLT63_07560"
     CDS             5184..7355
                     /locus_tag="PLT63_07560"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HOD98618.1"
                     /translation="MERLMTEKPALGRRNAEIIGTICFAAGLFLTLCLASYNPDDPSF
                     TRFYPGTVEVHNLIGAFGAYTSDTLIRIFGFSSLFFPVALFVCAGKFFLHSDFRITVS
                     HAGGALLFLLSCSGLAALIIREVPLYDHPLRAGGLIGDAVRSYLTYYFNLAGAMIILL
                     LMMVLASMFLIRLSLVALGRALFGCCQKGWGWMRARYERFGSPGKAASPPGERAAQAP
                     TATPPRIDEPPVAPSGGKEKPGKAKQTHFDFFSLPGTFNLPQLELLEERERRDTRMKK
                     DSLLTNARILERKLADFGVEGKVTEIHPGPIITLYELEPAPGVKINRITNLADDLALA
                     LKAASIRIIAPLPGKAVIGIEIPNLEREAVHLRDVLDSEVFSGARSKLPIALGVDIMG
                     SPVVQDLTRMPHLLIAGTTGSGKSVSLNAMICSILFRSAPEEVKFLMIDPKRIELSAY
                     EGIPHLIHPVVVDPKKAAQVLKWAVEEMERRYAFIGDMGGKSIEAYNKQAEKEKLPRL
                     PYIVIVIDELADLMLVAPRNVEESLARLAQMARAAGIHLIIATQRPSVDVITGVIKAN
                     FPTRISFQVSSKVDSRTILDQLGAEALLGAGDMLFIPPGTSKVTRIHGAFVTDREIGR
                     IVEFLKQQGLPDYDASLKEYESTLESPERDGEVFDEKYDEAVELVADLGQASISLVQR
                     YLKIGYNRAARIIERMEAEGVVGPSDGVKPRKVLAKKLPSR"
     gene            7426..8784
                     /gene="rimO"
                     /locus_tag="PLT63_07565"
     CDS             7426..8784
                     /gene="rimO"
                     /locus_tag="PLT63_07565"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOD98619.1"
                     /translation="MMPDREAKKFHIVSLGCPKNLVDSEVMAGILMRRGFRLTTDARE
                     AGIIIINTCAFILPAKEEAIEAILTAATYKKTAACHRLIVAGCLPQRHGAELARAIPE
                     VDLFIGTAEVPHIAEHLTGMATPRYDGPRVRTGRPDFLMHAGHPRRLMTPSYTAYLKI
                     AEGCGNSCSYCIIPNLRGPARSRTPEDIVAEARKLVGRGVKELILIAQDTTAYGRDLP
                     DRPSLTGLLRELAALEELVWIRLLYSHPAKITPELIRILNGDPKICPYLDMPIQHIDD
                     EILGAMNRRGNGRKIRSLLKTLRAEGPDIALRTSLIVGFPGETKKKFSELMDFVRTAR
                     FDHLGVFPYYREEGTAAAALPARIPAAEKERRRELIMDAQADISGQLLRRRLDTVETV
                     LIEGKSGQADYPFVGRTRRQAPEIDGITFVRGRGLRPGRFVSVRITAVTTYDLFGEVV
                     DN"
     gene            8843..9820
                     /locus_tag="PLT63_07570"
     CDS             8843..9820
                     /locus_tag="PLT63_07570"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOD98620.1"
                     /translation="MNDIQDVFKRYWDDLQRVEDYMSAALHSDISLIPQVSGHLIGSG
                     GKRFRPLLLMAAAALCGYQGERRHPLAAVIEFIHTSTLLHDDVIDNADMRRGKTAANH
                     VWGNAASVLVGDFLYSEAFRLMTEHGDMEIIRVLARATNLMAGGEVFQLMKKGDPEIT
                     EADHLTIIEKKTSHLISAACTVGGLLAGAPAGQIAALGRFGLDLGFAFQIIDDALDYT
                     ASADEFGKAIGKDLAEGKMTLPLIRTLQNCPAAERQMIVEIIRDQDRDETSLQVVFSI
                     VQRHDGIPYALNMAQNYIEAGKNQLEVFSDSSPRRDLMVLADYVVTRKF"
BASE COUNT         2035 a         3217 c         2740 g         1949 t
ORIGIN      
        1 agtgacgaag gatgaagcgc aacgccgcat atggactttt tacgatgtcg tcaaccttat
       61 ttcatccggc cgtcgcaacc ttcaggaccc gggtctggcc ttcagccccg ccgcgatggc
      121 ggccacgatc cggtccgcct cggcagccgg gtcaacggcc atgcggatcg gacgaccaac
      181 aaccaggtaa tccgcgccgg cggcaatcgc cgtttccggg gccagggtcc gtttctgatc
      241 atctccggca atggggtccg taccgcggat cccgggcgtc acgattacga aatcctcccc
      301 gcaaacggcc cggatggccc cgatctcccg gggtgaagcc acgacccccg ccagtccgga
      361 ctcccgagcc aagcaggcga aatttcgcac cgcatccaga gagccgatgc gaaaacctat
      421 ctcccggagg tcgtcatcat cgaggctggt caggacggtg acggcgatga ggaggggaag
      481 gggtttactc agtcgcgccg cctcgtcctg ggccgccgcc gccgccgccg ccatcatcct
      541 tcttcccccg gccgcatgga cattgaacat ggccgccccc aaccccaccg caccggcggc
      601 agcacgggcc acggtgttgg gaatgtcgtg atatttgaga tcgagaaaga cccgggcgcc
      661 ccgttcctga atcctttcca ccagggtcgg gccatagatc gtgtaggcct cctttcccac
      721 tttgaacagg ccgacgtgcc cctgtaagcg gtccacccag gcgagggcct catcaatgcc
      781 cccccggccg atgtccaaag cgaagatcaa ccgctcccgc gggaggtcac tccgtatatt
      841 cgtcatcttc tgtaaactcg gtctgatcaa taatcggccg aaaattctcc ttcgtgtact
      901 tcagattctc cggatgggca tacatcacct tcccaactgc gatctcccga agcgccgtga
      961 ctatttcacg gttgttccga ccttcctcca ggggtctgga ccccttcaga agctggatcg
     1021 cccgctggga agtgagcaac accagcgcaa agcggttctt ggcctggcgt aacgagtctt
     1081 cgacggttat tcttgccata gggatcagta tcctccttaa aaattacgcg acattatttc
     1141 caagcggcac gccccgttca cctctcggaa ggttttcatt gccggcgaca gaacagcggt
     1201 gggcatggtc accagaacat taatgaacag caatcacctg cggtgattta taaccataga
     1261 ataagcgtag cgtaggcgat tttactccgc caggaaaggg gatacgatcc ccatctgccg
     1321 ctcccgccgg catttctccg ccagatagat tgcccgcatc cgttcgatgg cctgttcgag
     1381 ccggtcgttg acgacaatgt aatcatacca gaaaacttcc tggatttcat ccctcgcttt
     1441 ctgataacgc cagcgccgca cttcctcgtc tccgtctccc cgcttccaca gacgccgctg
     1501 caattccgcc aggtccggag gaagaacgaa gacaaagacg cctttgggat agatctcctt
     1561 cagggcccgg gccccccggg gctccacgtc caggaggata tcctcccccc gcccgcggat
     1621 ttcctcgatc acgccccttg aggtcccgta gagatgaccg taattctccg cccattccac
     1681 gaaatcaccg gcctggatct tctcccggaa ggcgtccggg gtcacgaagt gataatcccg
     1741 tccatcagcc tcgccccggc ggggcgggcg ggtggtgaag gaaacggaaa accgcaaggc
     1801 cggaaactcc cggagcagcg ggcggcagat cgacgtcttg cccgttccgg aaggagccga
     1861 aactaccata atcactgaat tattcgacat tttgtatctg ttcccgcagt ttcgccagtt
     1921 cgctcttgat ctcgatgacc cggcgggcaa tctcaccgtc ctggcttttc gaaccgatgg
     1981 tgttgatctc ccggttcatc tcctggatga ggaaatccag cttacgcccc acggcctccc
     2041 cggccgttaa aaactccccg aactggttga tatggctctc cagccttacc acttcttcgt
     2101 tgatgtcact cttctcggcg aggatggcca cctcctgatg gaggcgggcc tcatcgatca
     2161 ccatgccctg ggtcaattcc cgaacccgct cggcgaggcg ctggcgatat tcctgcacca
     2221 ccgccggcga tcgccgggtt atatcttcga ggagaccgga gatcatctcc atccgggtcg
     2281 ccaggtcccg gcccaaagcc tccccttcag ctttcctcat ggcggcgagt ctgtccatgg
     2341 cctgcagcag cacccgctcc agccccgccc acagggccga cagattctcc tccgcctccc
     2401 cggggacata aaggtcccgg aatcctgtca tcatctccag ggtcacttct cccggaaggt
     2461 tcaactccgt cttgatccgg gtcagaaggg cgtgatactg acgcaccagc ggaaggttta
     2521 gggtcagtgc cggcgcctcc tcctgatggc catcgctctc caggcggatg gtgacctcga
     2581 tcttgccccg ggaaaagcgc tccccgatct ttttcttgat ctccgcctcc agcgccgcca
     2641 acgccgccgg cacgcggagg gatatttcca gataccgatg gttgagagac ttcatctcca
     2701 cctggatccg tttgccggcg gcagcaccct cctcccggcc gaacccggtc atacttctaa
     2761 tcatgatttt ccttcctgat cttttgcaac tgcccgagat atttcctccg gacagattgg
     2821 attatcgcca aggtctccgg ggccgcgtaa tcgggatacg tccagggcag gggccgaaac
     2881 gccccggccc tgtaaacgag ggtcaagtcg gcgtaaatac ccgagcgcag ataggggcgg
     2941 tggtcgtaac ccttgcccgt cgccaggatg agatgggcgg cggcgacgta gcccgggtcg
     3001 atgttcaccc gccgcgtctc cccggcgata agacgacgct ccaggtcatt ggtccagagc
     3061 tttatttccg gcagggactc cggggaaata tgtcccgcaa aggaaacgaa acgacgacgc
     3121 agtccccgcc ccatctcccg ctcataataa tccgtatagg caaactcccg gcaggggctg
     3181 atataatccg cctccccaaa gcgctccatg aggagggcga tggccgcggc aatgacctct
     3241 ccctccgtcc cgatgacgct cacaaaaaga tttaccggcg gggcgggacg ggggatgctc
     3301 ataaggtgcg cttcagttct tttctggtga ccgtcgccgt tcccacctta cccacggcaa
     3361 tgcccgccgc ccggttcgcc atgaaggccg cctcccggaa cgtcgccccc gaggccatgg
     3421 ccagggcaaa gacaccgatt gccgtgtcgc cggccccggt gacgtcgaaa acctcacgtg
     3481 cttccgcggg gaattccgta tggcggatcg tcccatcccg ttcgaaaagg ctcatcccct
     3541 cctcaccccg cgtaacgagc agggcacgga aaccatattt cctgatcaat tcccgcccag
     3601 ccttcaccag cgaggagcga tctgtgatct cgaagcccac ggcccgggat gcttcgtgat
     3661 ggttgggggt cacgatgtcg aagtcccggt agagagaaaa atcgttcttc ttgggatcta
     3721 cgcagacaac cactgtcgaa ccggcgaaga tgcggcggac gccctcaatc agggacggcg
     3781 tcaccacccc tttgctgtaa tcggaaatga ccaccgcccc caggtctccc cggaggctct
     3841 ccatatagtc aataatcccg gcaatcgcct cttcgctcac ggggacgcgg ctctcccggt
     3901 cgaaacgaac cacctgctgg ccttgggcaa cgatgcgggt cttcagggtc gtaggccggc
     3961 ggtcttcgac gaaaatcccc atcgtggcga tatccttcgc cgccagttcc gccagaagac
     4021 gccggcccgt gtcgtcgccg cctacaacgc ctaccccgta aacccggcct cccatggcgt
     4081 ggatattgtt catgacgttg gccgcgccgc cccagagcag gtgatcggag cggacatcca
     4141 caaccggcac cggggcctcg ggggaaatcc gcgccacctg accgcggatg aagtggtcca
     4201 ccatgatgtc cccgacgacc agtaccttcg cctgggtaaa ttgatccatg acttccagtg
     4261 ctcgtttctt agtaaacagt cggcgcataa aaaaggcgct ccttgacggt attttccaat
     4321 ttaaggaact tgtctgttta ttttaagaaa aggttttttg tcaatgacta aaatgtcccc
     4381 gtctttccca aacaaataca ttgacttcac ggcatatatc ctttaaatac aacatgtttc
     4441 gcaatacaat cacccatcgg gggaaacaag aggattatgt tccgttccat cctgctcgta
     4501 ttgataagtg ttataatcac ggcgtttttc tccacttgcg tcgttgtttt ctccctcctg
     4561 ggagcagcgg aaaactccct ccataaggtt gcgcggacct gggccaaggt cctcttggcc
     4621 atctccaatg tccaagtccg ggtgatcgga acggagtacg tggatgggga gacgccgcaa
     4681 atcttcatgt ccaatcatca aagcgacttt gacatcctga tcatcctggc ttttctaccc
     4741 ggccagttcc gctggatcgc caagaaggaa ctctttcgga tccccgtctt cggccgggcc
     4801 atgaaaaacg ccggctatat agagatcgac cgccagcatc atcagcgcgc cctgcaaaac
     4861 ctcgccgagg cggcccggaa gattcgggag gggaaatcgg tgatgagctt tcccgaaggc
     4921 acccggagca aggatggaag catcaaaccc ttcaaaaaag ggatgttcca tctggctatg
     4981 gaagcgggag tacccatcgt ccccattacg attatcggcg ccgacgagat tatgcccaag
     5041 cggtccctta aagtcaatcc cggccggatc accatgatta tcgacgcccc catcgacatc
     5101 gacgaataca ccgacgatgc tcggacggag ctgatggagc gggtccgggg catcatcgtg
     5161 aagaattttg acatgtggcg ggaatggaaa gactcatgac ggaaaaaccg gccctcggca
     5221 ggagaaacgc ggagataatc ggcaccatct gtttcgccgc cgggttgttt ctgactttgt
     5281 gcctcgcctc ctacaatccg gacgatcctt cctttacccg cttttatccc ggcacggtgg
     5341 aggtccacaa tctcatcggc gccttcggcg cctatacctc cgacaccctg atccggatct
     5401 tcggtttcag ctccctcttt tttcccgtgg cactgttcgt gtgcgcggga aaattctttc
     5461 tccatagcga tttccgcatc accgtctccc atgccggcgg cgccctgctt ttcctccttt
     5521 cctgctcggg attggccgcg ctgatcatcc gggaggttcc tctgtacgat cacccgctcc
     5581 gggccggcgg cctgatcggt gacgccgtac gttcctacct gacctactat ttcaatctcg
     5641 ccggcgccat gatcatcctt ctgctcatga tggtcctggc ctccatgttc ctgatccgcc
     5701 tgtcccttgt ggccctgggc cgggcgcttt tcggatgctg ccaaaaaggc tggggttgga
     5761 tgcgggcacg ctacgaacgg ttcgggagcc cgggcaaggc cgcttcgcca ccgggggaaa
     5821 gagccgcgca ggccccgacc gccacaccgc cccgcatcga cgaaccaccg gtggcaccat
     5881 cgggcggcaa ggaaaaaccc ggcaaggcaa agcagaccca cttcgacttc ttttccctgc
     5941 cgggaacctt caatctgccc cagttggagc tcctggagga acgggaacgc cgggacacgc
     6001 gtatgaagaa ggacagcctc ctcacgaacg cccggatact agagagaaag ctcgccgact
     6061 tcggcgtgga gggcaaggtt acagagatcc atcccggccc tatcatcacc ttatacgaac
     6121 tggaaccggc accgggggtc aagatcaacc ggatcaccaa tctggcggac gatctcgccc
     6181 tggccctgaa ggcggccagt atccgcatca tcgcccccct gccgggaaag gcggtgatcg
     6241 gcatcgaaat ccccaacctg gaaagggagg cggtgcacct gagggacgtc ctggattccg
     6301 aggtcttcag cggggcccgg agcaagctcc ccatcgccct gggggtggat atcatgggct
     6361 cccccgtggt ccaggatctg acccgcatgc cccatctcct catcgccggc accacgggat
     6421 cgggaaaaag cgtctccctt aacgccatga tctgcagcat cctcttccgc tccgcccccg
     6481 aagaggtcaa gttcctcatg atcgatccga agcgaatcga gctttccgcc tacgaaggga
     6541 ttccccatct catccatccc gtggttgtgg atcccaagaa ggcagcccag gtgctcaaat
     6601 gggcggtgga ggagatggag cggcgctatg ccttcatcgg cgacatgggc ggcaagagca
     6661 tcgaggctta caacaagcag gcggaaaagg aaaagctgcc ccgcctcccc tatatcgtca
     6721 ttgtcatcga cgaactggcc gacctcatgc tggtggcccc gcggaacgtg gaggaatccc
     6781 tggcgcgact cgcccagatg gcccgggcgg cggggatcca cctcatcatc gccacccagc
     6841 gtccctccgt ggacgtcatc accggggtca tcaaggccaa cttccccacc cgcatctcct
     6901 tccaggtctc ctccaaggtc gattcacgga ccatcctgga ccagttggga gcggaggccc
     6961 tgctgggcgc cggcgacatg ctcttcatcc ctccggggac ctcgaaggtt acccggattc
     7021 acggcgcttt cgtcacggac cgggagatcg gcaggatcgt ggaatttctc aagcagcagg
     7081 gactgccgga ctatgacgcc tcgctcaagg agtatgagtc caccctggaa agtcccgaac
     7141 gggacggcga ggtcttcgat gaaaaatacg acgaggcggt ggaactcgtc gccgacctgg
     7201 gccaagcttc catctccctg gtgcagcgct atcttaagat cgggtataat cgagctgccc
     7261 ggatcatcga acggatggag gcggaggggg tcgtggggcc ctcggacggc gtgaaaccac
     7321 gcaaggtgct ggccaagaag cttccctccc gatgaaggat tgcctgggtg gcgcaatata
     7381 ccctggacag atccccgggg ggaaaggggc agaaggtggc ggccgatgat gccggacagg
     7441 gaagcaaaga aattccacat cgtcagcctc ggctgcccga agaacctcgt cgattcggaa
     7501 gtcatggcag gcatcctgat gcgccggggt ttccgactca ccacggatgc tcgggaagcc
     7561 gggataatca tcatcaatac ctgcgccttc atcctacccg ccaaggaaga ggccattgag
     7621 gccatcctta cggccgcgac atataaaaag accgcggcct gccaccgttt gatcgtcgcc
     7681 ggctgcctgc cccagcgtca tggagcggaa ttggcccggg cgattccgga ggtggacctc
     7741 ttcatcggca ccgcggaagt tcctcacatt gccgagcacc tgactggcat ggctacacca
     7801 cgatacgatg gtccccgggt cagaaccggc cgaccggact ttctgatgca tgccggtcat
     7861 ccccgacgcc tgatgacgcc gtcctacacg gcctatttaa aaatcgccga gggttgcgga
     7921 aatagctgtt cttactgcat tattcccaat ctacgcggtc cggcccggag ccgcaccccc
     7981 gaagacatcg tagccgaagc ccgcaagctg gtcggccggg gggttaagga actgattctc
     8041 atcgcccagg acaccacggc ctacgggcgg gacctgcccg accgaccctc ccttaccgga
     8101 ctcctccggg aactggccgc tctggaagag ctggtctgga tccgcctcct ctattcccac
     8161 ccggcaaaaa tcaccccgga actcatccga atcctgaacg gggacccaaa gatctgtccc
     8221 tatctggaca tgccgatcca gcatatcgac gacgagattc tcggggccat gaaccgccgg
     8281 ggcaacggcc ggaagattcg atccctccta aagacgctcc gtgcggaagg tccggacatc
     8341 gccctgcgca cctccctcat cgtgggtttt cccggcgaaa caaagaagaa attcagtgag
     8401 ttgatggatt ttgtccgaac ggcgcgtttc gaccacctag gggtcttccc ctattaccgg
     8461 gaagagggca cggcagcggc agccctgcca gctcgaattc cggcggcgga gaaggagcgg
     8521 cggcgggaac tgatcatgga cgcccaagcg gacatctccg gccaactcct ccggcggcgg
     8581 ctagataccg tggaaacggt cctcatcgag ggtaaaagcg ggcaagccga ttatcccttc
     8641 gtgggccgaa cccgccgcca ggccccggag atcgacggca ttaccttcgt ccgggggagg
     8701 gggctccgcc ccggccgctt cgtttccgtc cgcatcaccg cggttaccac ctacgatctt
     8761 ttcggcgaag tcgttgacaa ttaaccctga aggaaatata aggtaaggac acgggcagga
     8821 atccaacccc cttggtttac ggatgaacga tattcaagat gttttcaaaa gatactggga
     8881 tgatcttcaa cgggtggaag actacatgtc cgccgccctg cattccgaca tcagcctgat
     8941 cccccaggtg agcggccatc tcatcggcag cggtggcaag cgttttcggc cgctgctcct
     9001 catggccgcc gccgccctat gcggttatca gggggaacga cgccaccccc tggccgccgt
     9061 aatcgaattc atccacacct ccacgctcct ccatgacgac gtcatcgaca acgccgacat
     9121 gcgccggggg aagacggccg cgaatcatgt gtggggtaac gccgccagcg ttcttgtcgg
     9181 tgattttctc tattcggagg cgttccggct catgacggag catggagata tggagatcat
     9241 ccgggtcctg gcccgggcca cgaacctcat ggccggcggg gaggtctttc agttgatgaa
     9301 aaagggcgat ccggagataa ccgaggcgga ccacctgacg atcatcgaaa agaagacctc
     9361 ccatctcatc tccgccgcct gcaccgtcgg gggattgttg gccggtgcgc cggcaggaca
     9421 gatcgccgcc ctgggccgct tcggcctcga tctggggttt gccttccaga tcatcgatga
     9481 cgccctggat tatacggcca gcgccgatga attcgggaag gccatcggca aagacctcgc
     9541 agaaggaaag atgaccctgc ccctgatccg gaccctccaa aactgcccgg cggcggagcg
     9601 gcaaatgatc gtcgagatca tccgggacca ggaccgggat gagacatccc ttcaggtggt
     9661 tttctccatc gtgcagaggc acgacggcat tccctacgcc ctgaatatgg cccagaacta
     9721 tatcgaagcg gggaaaaatc agctcgaagt cttttccgac tcctctcccc ggcgtgatct
     9781 catggtcctc gcggattacg tcgtcacacg aaaattttaa ccacaaatca cctacggtga
     9841 tttgtgacct acagcatcat tcaaggatag acttccatcc ccggcgccgc cccgccttcc
     9901 ccgtgtcggc gatcctccct aacggcctca ggcagaaagt c
//
LOCUS       DAOSMF010000070         9675 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_7969_length_9675_cov_18.594283, whole genome shotgun sequence.
ACCESSION   DAOSMF010000070 DAOSMF010000000
VERSION     DAOSMF010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 9675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9675
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7969_length_9675_cov_18.594283"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="PLT63_07575"
     CDS             <1..515
                     /locus_tag="PLT63_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98621.1"
                     /translation="FRFHPSFEKEGIPALSLKLIIGKRVLKKNIYQNRQGVPLFSANI
                     RNPFGFVHTANAGNLKFGGCLWSIDSDFDCRGVAAGESYSITDHCGQVEICSYQIDPH
                     YLARQIRQSGFDRGFNRDYRPSLGIMANLELDLPVNNNGEFDLVIMQEWTAFQEEIDR
                     VGEDLEKLMA"
     gene            complement(769..2886)
                     /locus_tag="PLT63_07580"
     CDS             complement(769..2886)
                     /locus_tag="PLT63_07580"
                     /inference="COORDINATES: protein motif:HMM:NF017973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOD98622.1"
                     /translation="MTKKIKTVIGALRYTVKTLAEKRGIILTVGGDTACTDGKRVNLP
                     VLPEDDETARLLAEGYVDHEAAHVKWTDNKVWDDYCKVKPKLCVNILEDVRIERLQGA
                     KYPGAMINLRRLLHEVVKQGGLAVPPPEDAVGRLMLWLIARERRLVLGQDALADRENT
                     VEGHCRKQYGNDFCDRFVALADQAAGCQSTQDCADLADEIRELIKNPPTPPPPPPPPP
                     PKPDPKSDPESGQDKGKDEEGDKGKEGQEGEDESKGSNADSSDDQEGEEQPEAGDSGS
                     DPDSSPDDGEPSAPSSSDGGNSQSDDGNDADEEGEDQNGSGGATGSGEEEEKGEDSNS
                     SSSSDSQADDGKDADPSPSGPQNAKGAGGEDADSDSTPDDGEPSDPSSSSDSSSDSSR
                     IAKALKALANAEEPEEGEAPDLGSQLAKALNEIVQTGAKGDGDTPLVIANESPNPRAD
                     VPLLPQQEDGMREAVVKTSRMTAQLAGLLQSARRKPSYPRQVGTRIDPRSCHLIAVNT
                     PENRVFASRREKVAVNTAVTLLIDRSRSMEWGRQHMLWPAMEATYSTSVALSRIEGVV
                     HSIACYPGMDGEEENVTVLKKFGERPDMRQYNGMYGDGGTPIAEALMWAGMVLTHRHL
                     EKRKIVVLFTDGEPNDDNLAAQAVGVLRKSGVEVYAVFFGKSTFREWIDEDSSRVLDD
                     IQELPKVFITLLKQTLLRKAKAA"
     gene            complement(2998..3957)
                     /locus_tag="PLT63_07585"
     CDS             complement(2998..3957)
                     /locus_tag="PLT63_07585"
                     /inference="COORDINATES: protein motif:HMM:NF022792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3150 domain-containing protein"
                     /protein_id="HOD98623.1"
                     /translation="MYKNILEQIVLLHLEMRVWSGRRKLDPEDLKLADGSELPPESLA
                     RLGSKKVIDPDRLNDLLTIRGDAHRLALATGTRLMGGYAIPIGKMAELAPKLNALMEK
                     SRELKNKLLEGLTEAIEEWKGKNPGWEALIERADISPEAVGKAISFSWQVFNIAPVEG
                     QEEGLEAEVTGLAGQLRREIETEARVLWKKSFQGKAEVTQRALRPIRAMVRKIHGLAF
                     LEPELEDLVNGICDAITALPQKGPIRGKDFASACGIVGLLGNIPNAVEEAEAVIAAAK
                     ADEEAEDQEGNLLEEEAADQTVTSPPFLSAGHAAVPAPPAEWF"
     gene            complement(4137..4568)
                     /locus_tag="PLT63_07590"
     CDS             complement(4137..4568)
                     /locus_tag="PLT63_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98624.1"
                     /translation="MYNKIAPPPGAEWGICLKRRGEYWIAFQTASAIEVRYGVKEPLV
                     EQTLPASPAIMKTLIKQREDKWFKMVAEWNPTELWVPRQPPSQPHSSPTTGGGKYRKH
                     GQLPPPPPNRPVPPPPPPKPMSIIDIVRAWQEEVPPEEEWF"
     gene            complement(4581..4970)
                     /locus_tag="PLT63_07595"
     CDS             complement(4581..4970)
                     /locus_tag="PLT63_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98625.1"
                     /translation="MSLPTNWPVTIPANAKAVVWMRSDSVSGGKDWIGIATDREFITH
                     WGKTGHVNQSRVKEKNGFLGSALSNLMAAEAGKSRKGYWVVARMTRDAPAPSLNDPPP
                     PPSKPSAIAKAVEAWQKEALATEGEWF"
     gene            complement(4987..5934)
                     /locus_tag="PLT63_07600"
     CDS             complement(4987..5934)
                     /locus_tag="PLT63_07600"
                     /inference="COORDINATES: protein motif:HMM:NF019348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOD98626.1"
                     /translation="MEESINKLFEGLKAPDAVKMEVREDTTGHPMVPRQKPYRFRQEP
                     MSDFLGWLKGASGFDPLLITGPTASGKSSLAEQVAARLNQPAYIVPCHERMEVPDLFG
                     RFVVRDGSMTWVDGPVIAGLKDPAGAWIVMDEFDSMEPGALLGLNPVLEGRKFIVPET
                     GELIDPVAHGAKIVCTGNTAGLGDQTGEYLATKRQNVATMGRFAVMEVGYPTKEEEIE
                     ILLLHRPDLPKPLGEGMIKVANLVREQYVAGEMECVFCTRSLVRWAHLALFFRRKKGV
                     NYIMHSLDRAIGFKYDPVIREALHQLAQRKFGNDAIIVN"
     gene            complement(6230..6610)
                     /locus_tag="PLT63_07605"
     CDS             complement(6230..6610)
                     /locus_tag="PLT63_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98627.1"
                     /translation="MAKLPPIQPPEESEWGVRTEGYNASGVLRDTAMFLTSNGEGIFV
                     GSFNDGCGKRRTNLIKAKDHREYIIRYLEARRNLGYQVTEIWTLGKGWQAHPATMPVE
                     VPQIRRVRLSTTLKWWQEQGPEWF"
     gene            complement(6623..7666)
                     /locus_tag="PLT63_07610"
     CDS             complement(6623..7666)
                     /locus_tag="PLT63_07610"
                     /inference="COORDINATES: protein motif:HMM:NF024554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HOD98628.1"
                     /translation="MTERRNGGLLGMINRLWQKMSRPKPRQQRRAKERISDHEVEEAR
                     RVNLAHLIESLGYKPRWFSAEKAMFSSPLREEKTPSFTVSYFKGRWGWKDWGTGDSGD
                     TISFVQAYYGLSFIEAVRKLNGGTYSSIPSSAPPPSARSEYDEDKIKFVRKLHSDRTS
                     LMSEKERLIIRLYFEDKGVAFYPEMAVVTYHSFRDKKSFVAIPMPHARCIKGLECREI
                     GGTDRKTLGNKTLWFLSRKSSKVLVTESILDALAGEIVLSDNSLSLLSINGVGNVGKL
                     DGVLAKLRPKEVLLALDNDHPGREAQKTAVRIASRYADRVIEIDHHVRAGAKDLHKLL
                     PPARQATPVARAE"
     gene            7932..9320
                     /locus_tag="PLT63_07615"
     CDS             7932..9320
                     /locus_tag="PLT63_07615"
                     /inference="COORDINATES: protein motif:HMM:NF014581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HOD98629.1"
                     /translation="MDDLSELLQINDRCSVSKHHSQITRLGWGVNLRDLNKEREIFIQ
                     DSERSGHFGCFGTTRIGKSKLMENIVEQDIKKGCNVVIIDPKGDPELLGRVCQAAAEC
                     GRLREVMMLNPIFPDSSIMLDPLAYYYMEEELINHIISGIKAKEDYFIGIAQEVSHAV
                     IAGFALKQRVKNEVEGKNEPMSINFLKVKQNSSWHTLTKFKESIASLPGSEDLVASME
                     QILSSPQDFFAKVSSSLRTTLSALTSGTTGKIIGKAEYNEFVRRIESGEGVILFCNTG
                     SMLTDRTAHIIGRVLLSMIKSMVGRVFASGRKLKPPLCLHLDEGHNILYRGVQELFNK
                     GGGANVWIHFYTQSISQMEEEVGLEVTTSIIDNINTWMYMLVNHPATAQYMEDSSPTK
                     KKFQSILSLGGGISLRETEEKQILASKVLQLPKRHFYLRTYGELYRGITHDVSPLMVE
                     VEMPRIAGSSPA"
     gene            complement(9324..>9675)
                     /gene="traN"
                     /locus_tag="PLT63_07620"
     CDS             complement(9324..>9675)
                     /gene="traN"
                     /locus_tag="PLT63_07620"
                     /inference="COORDINATES: protein motif:HMM:NF018666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="conjugal transfer protein TraN"
                     /protein_id="HOD98630.1"
                     /translation="GSYLGFNLCHFVGDLKTGDFLGMKLKSRNVYCCFNSILARLVHE
                     QGRPQVNRGWGSAEQPDCAGFSIGEFAALDFSQMNLAEYMQYVQTKSNISPAEMEAIK
                     ERAITSAGAGSPPP"
BASE COUNT         1944 a         2795 c         2675 g         2261 t
ORIGIN      
        1 cgtttcgctt tcatccctcc tttgaaaaag agggaattcc cgctttatct cttaagctta
       61 ttatagggaa aagggtgctt aagaaaaaca tttatcagaa ccggcagggt gtgccgcttt
      121 tctccgcaaa catacgtaac ccttttggat ttgtacatac tgcaaacgca ggcaacctga
      181 agttcggagg atgtctttgg agcatagatt cggactttga ttgccgaggt gtggctgcgg
      241 gtgagtctta ttcgatcaca gatcactgtg gccaagtgga aatatgctct taccaaatcg
      301 atccacatta tctcgcacgg cagattcgtc aatctgggtt tgaccgagga tttaaccgag
      361 attatagacc atctttggga attatggcta atttggagtt ggacttgcct gtcaataaca
      421 acggtgaatt cgatcttgtt attatgcagg aatggactgc atttcaggaa gagattgatc
      481 gagttggtga agacttagag aaactgatgg catgaggttc aaaaatggtg caacctttcc
      541 ttctatcatc aattcttgaa actgccggat ctaaagctta taagagaata tctctcgaga
      601 gaacctggat taaccggcaa gcccctcaac cgtggaattc cgaaaaccca gacgaccttg
      661 gatcttgagg aaaaagaatc ggatcaggca tcgttattct aatcgttttt gttttaggcc
      721 aaaaaaaagg cgcccggtat gaaaccggac gccttttgcg tgtgctgctc aggccgcctt
      781 cgcctttcgg agaagggtct gcttgagcag cgttataaat actttcggca attcctggat
      841 gtcgtcgagg accctgctgc tgtcctcgtc gatccattct cggaatgtgc tcttgccgaa
      901 gaatacggcg tacacctcta cgccgctctt tcggagaaca ccgaccgcct gtgctgccag
      961 attatcgtcg ttcggctctc cgtcggtgaa gagcacgaca atctttcttt tctcgaggtg
     1021 gcgatgggtc agtaccatgc cggcccacat gagggcttcg gctatgggcg ttcccccgtc
     1081 accgtacatg ccgttatact ggcgcatatc cggcctttcc ccgaatttct tcaggacagt
     1141 gacgttttct tcttcaccgt ccatccccgg atagcaggcg atgctgtgga ccacgccttc
     1201 tatccggctc agagcaacgc ttgttgagta cgtggcctcc atggccggcc acaacatgtg
     1261 ttgccttccc cattccattg acctgctccg gtcaatcaga agcgttaccg ctgtgttgac
     1321 cgccaccttt tcccgccgag aagcaaacac ccggttttcc ggggtgttca cggcgatgag
     1381 gtggcaggaa cgcggatcaa tgcgcgttcc gacctggcgg ggataggacg gcttccgcct
     1441 cgccgactgt agcaggccgg cgagctgcgc cgtcatgcgc gacgtcttga cgaccgcctc
     1501 gcgcatccca tcctcttgct gtgggagcaa ggggacgtcc gctcgggggt tcggcgattc
     1561 attcgctata acaagcggag tatcgccgtc gcccttggca cctgtctgaa caatctcatt
     1621 cagggccttg gcgagctgag aacccaggtc tggggcctcc ccttcctctg gttcctcggc
     1681 gttcgccagg gccttgagag ccttggctat gcgcgaagaa tcggaggaag aatcggagga
     1741 ggaggaggga tcggacggtt cgccgtcgtc aggcgtagaa tcagaatcgg catcctcccc
     1801 gccagcccct ttagcgtttt ggggtccgga tggagacgga tcggcgtcct tcccgtcgtc
     1861 agcctgggag tcggaggatg acgacgaatt gctgtcctcg cctttctcct cctcctcacc
     1921 ggaaccagtg gcgccgccgg agccgttctg gtcctcgcct tcctcgtcgg cgtcgttccc
     1981 gtcgtcggac tgggaattac cgccatcgga ggaggaggga gcggacggtt cgccgtcgtc
     2041 aggcgatgaa tcgggatcgc tccctgagtc gccggcttcc ggttgctcct cgccttcctg
     2101 gtcgtcagag gaatcggcat tgctgccctt gctttcatcc tcgccttcct ggccttcttt
     2161 gcccttgtcg ccttcctcat cctttccctt gtcttgacca gactcggggt cggacttggg
     2221 atcaggtttt ggtgggggag gcggcggtgg gggagggggc gtcggcggat tcttgatcaa
     2281 ctccctgatc tcgtccgcca ggtctgcaca gtcctgcgtg gactggcatc ccgccgcctg
     2341 gtccgccagg gcgacgaacc tgtcgcagaa gtcattgccg tactgcttcc ggcaatgccc
     2401 ttccacggtg ttctccctgt ccgccagggc gtcctgcccc aggaccaacc gccgttcgcg
     2461 ggcgatcagc cagagcatga gtcgcccgac agcatcctcc gggggaggga cggcaagtcc
     2521 tccctgtttg acgacttcat ggagaagccg tcggaggttt atcatcgccc ccggatattt
     2581 cgcgccctgg aggcgctcta tgcgaacgtc ctccaggata ttcacacaaa gcttgggttt
     2641 gactttgcag tagtcatccc aaactttatt gtcggtccac ttaacgtggg ccgcctcatg
     2701 gtcaacgtag ccctcggcca gaaggcgggc cgtctcgtca tcttccggca ggaccgggag
     2761 gttgacgcgc ttaccgtctg tacaggcggt gtcgccgcca acggtaagga tgatgccccg
     2821 cttctcggcg agggttttga cggtgtaccg gagggcgccg atcaccgttt ttattttttt
     2881 tgtcattttt cctcctttgc catgcctgcc cttacgggtt caggacttac aggcaggaaa
     2941 gtaatgtaat gtatggggga atgggtaacg cttccgccag ccacgccccc gtttcagtta
     3001 aaaccactca gccggcgggg ccggcaccgc agcatgtccc gcactcagaa agggagggga
     3061 agttaccgtc tggtcggcag cttcctcttc caggaggttg ccctcctggt cctccgcttc
     3121 ctcatcggct ttagcagcag cgataaccgc ttccgcttct tcaaccgcgt tgggaatgtt
     3181 tccgaggagg ccgacaattc cacacgcgga ggcaaagtcc ttcccacgga tgggaccttt
     3241 ctggggaagc gccgtaatgg catcgcaaat gccgttcacc agatcttcca gttccggctc
     3301 gaggaaggcc aagccgtgga tcttgcgaac catagcccgg atgggccgga gggccctttg
     3361 agttacctcc gccttcccct ggaacgactt cttccagagg acacgggcct cggtttcgat
     3421 ttcccggcga agttggccgg caaggccggt aacctccgcc tcaaggcctt cctcctgccc
     3481 ctcgacaggt gcgatgttga acacctgcca ggaaaaggaa atggcctttc caacggcttc
     3541 cggggagatg tcggcccgct cgatcaaggc ttcccacccc ggattttttc ccttccactc
     3601 ctcaatggcc tccgtcaggc cttcaaggag tttgttttta agctcgcgac tcttttccat
     3661 aagagcgtta agcttcgggg ccagttccgc catcttccca atgggaatgg cgtaaccgcc
     3721 catgaggcgg gtccctgtcg ccagggcgag gcgatgggca tctcccctga tcgttagaag
     3781 gtcattcaac cggtccgggt cgatgacctt cttggatccc aggcgggcca gggactctgg
     3841 ggggagttcc gacccgtcgg ccagtttgag gtcttccggg tccagtttgc gccggcccga
     3901 ccagaccctc atttcgagat ggagcagaac tatctgctca aggatgtttt tatacatatt
     3961 tcctcctttg ccatgcctgc ctctttcgag gctcaggagc ttacaggcgg ggaaacaaat
     4021 gccaaagctg tgagcacacc ccgtgcggga tgctatagcg ccccgcaggt acgcgcccag
     4081 gttttgacat tattataatg tttagggttt ttccggggcc ttggacaaag ccccgtttag
     4141 aaccattcct cttccggtgg cacctcttcc tgccacgccc gcactatgtc gatgatcgac
     4201 atgggcttcg gcggaggagg gggcggtaca gggcggtttg gcgggggagg gggaagctgt
     4261 ccgtgcttcc ggtacttccc cccgcccgtt gttggtgagg agtggggttg acttggcggc
     4321 tgtcgtggaa cccacagttc tgtcggattc cattcggcca ccattttgaa ccacttgtcc
     4381 tccctctgct tgatcagagt tttcatgatc gctggagagg cggggagcgt ttgctccaca
     4441 agcggttctt tcaccccata tctgacctcg atggcggagg cggtttggaa tgctatccaa
     4501 tattcgccgc gccttttgag acagattccc cattcagcac cagggggagg ggctatttta
     4561 ttgtacatgc cccacctcct ttagaaccac tcgccttcgg tggcgagtgc ttccttttgc
     4621 caggcctcga cagccttagc tatcgcggac ggcttggagg gaggaggggg aggatcgttg
     4681 agggaaggag ccggggcatc acgggtcatt cttgccacga cccagtaccc ctttcggctt
     4741 ttgccggcct cggcagccat caggttgctg agagccgagc ctaagaaccc atttttctcc
     4801 ttaacccgcg actgattgac atggccagtt ttaccccagt gagtaataaa ctctctgtcg
     4861 gtggcaatgc cgatccagtc cttgccgccg gacacggagt cactccgcat ccagacgact
     4921 gctttagcat tggccggaat ggttaccggc cagtttgtgg gaagggacat ggtatcaccc
     4981 ccctccctaa ttcacaatga tggcgtcgtt cccgaacttg cgttgcgcaa gctggtgcaa
     5041 cgcctccctg attaccgggt catatttgaa cccgatagcc cggtccaggg agtgcatgat
     5101 gtaattcact ccctttttcc gccgaaagaa gagcgccagg tgtgcccatc gcaccaggga
     5161 cctggtgcag aagacgcact ccatctcgcc cgccacgtat tgctcgcgga cgagattggc
     5221 gaccttgatc attccctccc caagaggctt ggggaggtcc gggcggtgta acaggaggat
     5281 ttcgatttcc tcctccttgg tgggataacc cacctccatc acggcaaacc gtcccatggt
     5341 ggcgacgttc tgccgtttgg tggcgaggta ctctccggtc tgatcgccca gaccggcagt
     5401 gttgccggta cagacgatct tcgccccgtg ggcaacgggg tcgatgagtt ctccggtttc
     5461 gggaacgatg aacttccgtc cctccagcac cgggttaaga cccaacagag cgcccggctc
     5521 cattgagtcg aactcatcca tgacgatcca ggcaccggca gggtctttca accccgcgat
     5581 gaccggacca tcaacccagg tcatcgaccc gtcccggacg acgaagcgcc cgaacaagtc
     5641 gggcacctcc atacgttcgt gacagggcac gatgtaggcg ggctgattca gccgtgccgc
     5701 cacctgctcg gccaggctgg atttgccgga ggccgtgggg ccggtgatga ggagcgggtc
     5761 aaacccgctg gcccctttca gccaacccag gaaatcagac atcggctcct gacggaaccg
     5821 ataaggtttc tgcctgggca ccatgggatg cccggtcgtg tcctcccgga cctccatttt
     5881 tactgcatca ggggctttga ggccctcgaa cagtttattt attgactctt ccatcttttc
     5941 ctccttgcca tgcctgccat ttctgggtca ggacttacag acgagaaaaa cacgcactac
     6001 gccgctgtcg cgggcgtggg cgccaggaac tcttgaaatt actttctcat ccctggaaac
     6061 tcagcgccgc aacacggcct ctggtcaact tatgtggtta gatttggttc cccgaattgc
     6121 aactgtgaac acatccccca cgggatgcta atagcgcccc gcaggatcac gctcaggttg
     6181 aagaggggag ctatcggatt ttacgggggc ggcaggacgc ctccccgttt tagaaccatt
     6241 cggggccttg ctcctgccac cacttcaacg tcgtggacag cctgacccgc ctgatttgcg
     6301 ggacctccac tggcattgtg gcaggatggg cttgccatcc ttttccgaga gtccatattt
     6361 cggtgacctg atagccgaga tttctcctgg cctccaggta tcttattatg tactccctat
     6421 ggtcttttgc cttaatgagg tttgtgcggc gctttccgca gccatcatta aagctcccga
     6481 cgaatattcc ctccccattg gaggtcagga acatcgccgt gtctcgcagg acgcccgaag
     6541 cgttatagcc ttcggttcgg acaccccatt ccgactcttc gggcggttgt atcggtggca
     6601 gcttcgccat cggatacccc gcctactccg cccttgcgac aggtgttgct tgtcgcgccg
     6661 gcggtaacag cttgtgcagg tctttcgcgc ctgcacggac atggtgatca atttcgatca
     6721 ctctatccgc gtagcgcgat gcaatccgca ccgctgtttt ttgggcctca cggcccggat
     6781 ggtcattgtc cagggcgagc aggacctctt tgggtctcag ctttgccagg acgccatcca
     6841 gcttcccgac gtttccgacg ccgttgatgg acaacagtga cagggaattg tcggaaagga
     6901 cgatctcgcc ggccagggcg tcgaggatcg actccgtgac cagcactttg gatgacttcc
     6961 gggacaagaa ccataaggtc ttgttcccga gggtcttcct gtccgtgccg ccgatctccc
     7021 gacactcaag ccccttgatg cagcgggcgt gaggcatcgg aatggcgacg aacgactttt
     7081 tatccctgaa ggagtggtac gtgaccaccg ccatttcggg ataaaaagcc acccctttgt
     7141 cctcgaagta gagccggatt atcaggcgct ccttttcgga catcagggac gtgcggtcgc
     7201 tgtgcagctt ccgcacgaac ttgattttat cctcgtcgta ttccgaccgg gccgatggcg
     7261 gcggggcgga cgacgggatg ctgctgtacg tgccgccgtt cagcttcctg acggcctcga
     7321 tgaagctgag gccgtagtaa gcttggacga aggagattgt gtccccggaa tctccggttc
     7381 cccaatcctt ccaaccccac cgccccttga agtacgagac ggtgaacgac ggcgtttttt
     7441 cctcccggag aggcgacgaa aacatcgcct tctccgcgct gaaccacctc ggcttgtagc
     7501 cgaggctttc gatcaggtgg gcgaggttga cccttcgggc ctcctcgacc tcatgatcgg
     7561 aaatgcgttc ttttgcccgg cgctgttgcc ggggtttggg ccgtgacatc ttctgccaca
     7621 gccggttaat cattcccaac aacccgccgt tcctcctttc cgtcatttat cctcctttgc
     7681 catgcctgcc cttgcgggtt caggagctta caggctgacc ctacgttccg cgcctgcccc
     7741 cggagagggt caggtcttgc cgaaacgcaa aaaaggcgca aatcatccat tggcatttct
     7801 gcatcaatgg cgactcgcgc ccttttcggt ttccctacga aaataactaa ctgcctatat
     7861 aatacttccc ggaaagggga aaatgacgaa aatcatggca ggggaagtaa tatacgagca
     7921 tggaggaatg tatggacgat ctatctgaat tgcttcaaat caatgacagg tgctcggtga
     7981 gcaagcatca cagccagata acccgcttag gatggggggt aaatctacgg gatctcaaca
     8041 aggaacgaga aatctttatc caggattccg aacgtagcgg ccatttcggc tgttttggca
     8101 ccacgcggat cggcaagtcc aagctgatgg agaatatcgt cgagcaagac atcaagaagg
     8161 gctgtaacgt agtcatcatt gatccaaagg gcgatcccga gctactgggc cgggtatgcc
     8221 aggctgctgc cgaatgcgga aggctcaggg aggtcatgat gctgaatccg atttttccgg
     8281 acagctctat catgctcgat cccctggcat attattatat ggaggaggaa ctgattaacc
     8341 acatcatctc cgggataaag gcaaaagaag actacttcat tggcattgcg caggaggtgt
     8401 cacacgccgt catagcagga ttcgccctca agcagcgggt caagaacgaa gtggagggaa
     8461 aaaatgaacc gatgtccata aacttcctca aggtaaagca aaactcatct tggcatacgc
     8521 tgacgaaatt caaggaatca atcgcatccc ttccgggcag cgaggacttg gtggcaagca
     8581 tggagcagat attgtcgtcg ccccaggact ttttcgccaa agtgtcgtct tcgctcagaa
     8641 ccacgctatc agccctgact tcgggcacga cggggaaaat cattggcaag gccgaatata
     8701 acgaattcgt aagacgcata gaatccggcg agggtgtgat cctattctgc aataccggct
     8761 ccatgctgac tgatcgcacg gcccacatta tcgggcgggt tttgctgtcg atgatcaaga
     8821 gcatggtcgg gcgcgtattc gcctccggac ggaagctcaa gcctccgttg tgtttgcact
     8881 tggacgaagg ccacaacatc ctgtaccggg gtgtgcaaga gctattcaac aagggcggtg
     8941 gcgccaatgt ttggattcac ttctatacgc agagcatatc acaaatggaa gaggaagtcg
     9001 gcctggaggt tacaaccagc atcatcgaca acatcaacac ctggatgtat atgctcgtca
     9061 atcatcccgc cacggcgcaa tacatggagg attcgtctcc tacaaagaag aagtttcaat
     9121 ccatcctgtc cctcggaggc ggcatatccc tgcgtgaaac tgaagaaaag cagatattgg
     9181 cgtcaaaggt gcttcagttg ccgaagcgcc acttctacct gcggacatac ggcgaacttt
     9241 accggggcat tacccatgat gtaagtccgt tgatggtcga agttgaaatg ccgcgcattg
     9301 cgggatcgtc cccggcgtga tccttatggg ggaggacttc cggcgcctgc cgacgttatc
     9361 gcccgctcct tgatggcctc catctcggcg ggggatatgt tcgattttgt ctgaacgtac
     9421 tgcatgtact ccgctaaatt catttgcgag aaatccaagg cagcgaattc cccaatggag
     9481 aaccccgcgc aatcaggctg ttccgccgag ccccacccac gattgacctg tggccggcct
     9541 tgctcatgga caagacgggc aagaatggag ttgaagcaac aataaacatt gcggcttttc
     9601 agcttcatcc ccaggaaatc gcccgttttg agatcgccta caaaatggca aaggttgaac
     9661 cccaagtatg aacct
//
LOCUS       DAOSMF010000071         9638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_8002_length_9638_cov_24.248252, whole genome shotgun sequence.
ACCESSION   DAOSMF010000071 DAOSMF010000000
VERSION     DAOSMF010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 9638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9638
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8002_length_9638_cov_24.248252"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="PLT63_07625"
     CDS             complement(<1..567)
                     /locus_tag="PLT63_07625"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006680776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOD98631.1"
                     /translation="MWFLRDLQFAHPAETVALLQDTGVDPHGIAAMQPKMQHLNIHLE
                     GVSCPAANIIKQEMLAVGGDAAVARGAVSCRLPATDVVLMGTRKQLGRFAGKIAGQPF
                     GLSVLAERLKGLIEKIDRDSWKLITPRREIAVGEKTLVMGIVNVTPDSFSDGGRYLEH
                     DRAVDHALRLVAEGADVIDIGGESSRPGA"
     gene            complement(579..2399)
                     /gene="ftsH"
                     /locus_tag="PLT63_07630"
     CDS             complement(579..2399)
                     /gene="ftsH"
                     /locus_tag="PLT63_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOD98632.1"
                     /translation="MNQLQKNIALWLVISLVFVLLYHLFTQPKGQQESVVYSDFVAYV
                     ERGQVREVTIQGDNITGKLTDGKNFKTFMPKDATIVDKLKAANVRIAAKPAEDSPWYM
                     TILISWFPMLLLIGVWIFFMRQMQGGGGKAMAFGKSRARLMTDKTNKVTFEDVAGIDE
                     AKAELQEVIEFLRDPKKFSRLGGRIPKGLLLVGSPGTGKTLLARAIAGEADVPFLSIS
                     GSDFVEMFVGVGASRVRDLFNQAKKHAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTL
                     NQLLVEMDGFESNEGVILVSATNRPDVLDPALLRPGRFDRQVVVPLPDVKGREKILAV
                     HARKVPIDNDVDFQVIARGTPGFSGADIENIINEAALYAARTNKEKVSMADFEFAKDK
                     ILMGTERRSMVISDEEKRNTAYHEAGHALIARLLPGTDPIHKVTIIPRGRALGLTQQL
                     PIDEKHTYRRRYLLNSIAILLGGRAAEELVLNDFTTGAGNDIERATALARKMVCEWGM
                     SETMGPLSYGKKEEAIFLGREFATHKDYSEETAQKIDAEVARIVSDRYEEAKKLLTEN
                     LDTLNRLAAELLEKEVLNATEIDAIIGIPMRETEPDNAEV"
     gene            complement(2482..3972)
                     /gene="tilS"
                     /locus_tag="PLT63_07635"
     CDS             complement(2482..3972)
                     /gene="tilS"
                     /locus_tag="PLT63_07635"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOD98633.1"
                     /translation="MLKKVRKAIEEFALLSDGDTVLVALSGGPDSVALLAVLRMLAEE
                     YGVSVAAAHLNHGLRGEAEAEECFVRELCSRRAVPLTVERADIQARKKAAGRSTEDMA
                     RVVRYEFLERVAATVGAHRIALGHHLQDQAETVLMNLFRGSGSEGLKGMTPMRDGLYI
                     RPFLHIERREIMAFLTQEGLDCVMDRSNDDRRHLRNRIRHDLLPLLRRDYNRNIDRAL
                     ARTAAVLKEEDEFFKSQVKRLLTAWNIAVTPEGFSLEVEKIRVQPVALSRRLVKHLLE
                     RLTDVEQGIFFHHVQAVLELAFAPRPSGRLHLPHGLEAKRDYGRLILARPETGGRDFA
                     FAYTVATPGELTIRETGDILTFRISEDFDCRRWEGAGDRQGCRERTRPLPGAKDRANC
                     GDSRPETDDQVTATACLDGDRTGTPVMVRNFRAGDRMRPLGMAGEKKLQDIFVDGKIG
                     RNERKRLPLIVADKRIAWIPGVCVHEEFKITSETKRILRIDYRRPA"
     gene            complement(4000..4644)
                     /locus_tag="PLT63_07640"
     CDS             complement(4000..4644)
                     /locus_tag="PLT63_07640"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOD98634.1"
                     /translation="MIKIDLFVFDLDGTLADTGQDIAAAVNHTLEKLGMATLEHKRIM
                     NYVGDGVQELIRRSLGTDHHYALAEALTVFRAYYGEHMLDKTALYPGVEECLAHFAGK
                     KKMVISNKKHEFTVKIVKSLGIAHYFDDIIGGDFRPYLKPDARLVDHYLDHYHIPREK
                     AAIVGDGHNDILLAKNAGIISCAFLNGFGERQRLLALRPDVTCENLAELKNIFC"
     gene            4745..5719
                     /locus_tag="PLT63_07645"
     CDS             4745..5719
                     /locus_tag="PLT63_07645"
                     /inference="COORDINATES: protein motif:HMM:NF016190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44 domain-containing protein"
                     /protein_id="HOD98635.1"
                     /translation="MEKRPWTKYGAFLVIALFMAVFVLELDAYARVGGGRSFGSRGSR
                     SAFSTSKPSPSQPSSPTRSMTSPSPASPQSPQRGGFLRTMGAGLAGGLLGGLLFSSLG
                     FGHGGGFGGGIGLFDIILIGGILYGIYWFLKRRRQQEAMATSQTSFYQASGLHAQALP
                     SNAQPVDVSSRDDYGADHRQGLTHIRLMDPQFNEDNFQTLCMDIFFKIQGAWANRDMA
                     SARSLLTDEMFRILQSEAEQMQRDKKFNKLENIAVRSVEIAEAWQEEGRDYITVRFLA
                     NLLDYTVSERGELLDGSKTDPVKFEEYWTFVRPVGNNPWQLSGIDQGR"
     gene            5720..6598
                     /locus_tag="PLT63_07650"
     CDS             5720..6598
                     /locus_tag="PLT63_07650"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HOD98636.1"
                     /translation="MKPKDRIAIVGLGKVGTAMGYLLKRAGYPIAAIADRNPAALAKA
                     HPYTGGRICPTIAAAPQDADAVLITTTDDAIASACRELAGALHPGQKVIHCSGAGSLD
                     LLISARESGALIASIHPIQSFAHVEGAIDNIPGSTFGITAGEEIREWAVDFVLALDGA
                     PFFVPDTDKPLYHAAACIASNYLTTLLYMVEEIYLHLGLNREEAIRAFWPLVRGTLRN
                     IESRGTVEALTGPIARGDIGTVEKHLLAFQDKLPEFLPAYRALGITATDLGLKKRSLM
                     EEKANMIKQLLQGGKT"
     gene            6595..7413
                     /gene="panB"
                     /locus_tag="PLT63_07655"
     CDS             6595..7413
                     /gene="panB"
                     /locus_tag="PLT63_07655"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041584852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOD98637.1"
                     /translation="MSTQVQITRVTTAKIRDMKKKGEKITMLTAYDYPTAVAMDSAGV
                     DIFLVGDSLGMVVLGYDSTLPVTMEDMLHHTKAVCRGTQRAMVIGDMPFMSYQTSVAE
                     GVRNAGRFLQEAGAQAVKLEGGREVAEVTSRIAAAGIPVMAHIGLTPQSVHQLGGYKV
                     QGKGDKAAQRLLEDAKILEEAGAFSIVLECVPKGLAKEITAALEIPTIGIGAGVDCDG
                     QVLVVHDMLGLFERFTPKFVKQYARINDHIREAVEKYCAEVKSGTFPDDAHSFM"
     assembly_gap    7502..7601
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7783..8739)
                     /locus_tag="PLT63_07660"
     CDS             complement(7783..8739)
                     /locus_tag="PLT63_07660"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOD98638.1"
                     /translation="MKKRLLCMMILLVAVLVSCASTQSSRYREGSNVGQETGMTVEDE
                     RRLTEEALPKMLKDYPPAQNKELQKYVSDLGMKIVKANNLEGNPYHYDFTVVDVTDVN
                     AFAMPAGKIFVTAPLIAAASNEAELAGVISHEIGHVVARHSAERMYAMEKEGKKTWMY
                     AAGGAVLGAAVGYGLGRMICGPDDTACHAKAALLGGAVGAGGGMLAQKYTFLVNSRED
                     EMEADRLGFKYAVGAGYDKDQVGKFYEKLLAMEKSASKGGGGISKSLSDAMSTHPPSE
                     ERVQQMHELAATTPAGKTVVNTTSFNRAKQIAAGMGKKSRQP"
     gene            complement(8919..>9638)
                     /locus_tag="PLT63_07665"
     CDS             complement(8919..>9638)
                     /locus_tag="PLT63_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase cyclase
                     subunit"
                     /protein_id="HOD98639.1"
                     /translation="RVVKGVHFVDIRDAGDPVENAAFYEREGADELAMLDIAATVENR
                     KTRLEWVKSVAAVITIPLTVGGGIDSLDDIELVLAAGANAVSMNSAAVRNPELVREAV
                     KKFGSEKITVAIDARRNPALPSGFELVVAGGTRPVGKDAIAWAGQCQELGAGVILPTS
                     MDGDGALTGYDLEFIQTIADVVTVPVVASGGAGKLEHFHDAVVKGGASILLAASVFHF
                     RTFSIRQVKEYLRDRGLTVRL"
BASE COUNT         1945 a         2980 c         2595 g         2018 t
ORIGIN      
        1 ggcgccggga cgcgacgatt ccccaccgat gtcgatcaca tcggcgcctt ccgccaccag
       61 acggagggca tggtccacgg cgcgatcatg ttccaggtac cttcctccgt cagagaagga
      121 gtcgggcgtc acattgacta tacccatgac cagtgtcttt tcccccacgg cgatttcccg
      181 gcggggagtg atcaatttcc aggagtcacg gtcgattttt tcgatcagtc ccttcaacct
      241 ttccgcaagc accgacagcc cgaatggttg acccgcgatc ttcccggcga accttcccag
      301 ttgtttcctc gtgcccatga ggaccacatc cgtagcggga agccggcagg acaccgcacc
      361 ccgggccacg gcggcatccc cgcccacagc gagcatttcc tgcttgatga tgtttgctgc
      421 cgggcaggaa accccctcca ggtgaatgtt gaggtgctgc atcttcggct gcatggccgc
      481 gataccgtgg gggtcaacac cggtatcttg aaggagggcg acggtctcgg cagggtgggc
      541 aaactgcagg tctcttaaaa accacatagg gaaatcattc agacttctgc attatccggt
      601 tcggtctccc gcatggggat gccgatgatg gcatcgatct ccgtggcgtt aagaacctct
      661 ttttccaaca gttccgctgc caggcgattg agggtgtcca gattctccgt caggagtttt
      721 ttggcctcct cgtaacgatc cgaaacgatg cgggcgacct cggcgtcgat cttctgggcg
      781 gtctcttcac tgtaatcctt gtgggtggca aactcccggc caaggaagat ggcctcttcc
      841 ttttttccgt aactcaaggg acccatcgtt tcgctcattc cccactcgca gaccattttc
      901 cgcgccagcg ccgtagcccg ttcgatgtcg ttgcccgctc cggtcgtgaa atcgttcagc
      961 accagttctt ccgccgcccg tccccccagg aggatggcga tgctgttcag aaggtatcga
     1021 cggcgatagg tatgcttttc atcaatgggc aactgctggg tcaaaccgag ggccctgccg
     1081 cgggggatga tcgtcacctt gtggatggga tccgttcccg gcagcagcct ggcgatcaga
     1141 gcatgaccgg cttcatgata ggccgtattg cgcttctcct catcgctgat caccatgctc
     1201 cgccgctccg tgcccatgag gatcttgtcc ttggcgaatt cgaaatccgc catgctcacc
     1261 ttctccttgt tggtccgggc ggcatagaga gccgcttcat tgatgatgtt ctcgatatcg
     1321 gcgccggaaa aacccggcgt cccccgggca atgacctgga agtccacatc gttgtcgatg
     1381 ggcaccttac gggcgtggac ggccaggatc ttctctcgtc ccttcacgtc cggcagggga
     1441 acgacgacct ggcggtcgaa acgtcccggc cgcaacagcg ccggatccag aacatcgggg
     1501 cggttcgtcg ccgacacgag aatcaccccc tcattggatt cgaaaccgtc catctccacg
     1561 aggagttgat tgagggtctg ctcccgctca tcgtggccgc cgccgagacc ggcgccacgc
     1621 tgtcggccca cggcgtctat ttcatcgata aagataatgc agggggcatg ttttttcgcc
     1681 tggttgaaga ggtcccggac ccgggaggcc ccgacgccga caaacatctc cacgaagtcg
     1741 gaaccgctga tggacaggaa tggcacatcc gcctcgccgg caatggcccg agccaatagg
     1801 gtcttccccg ttcccgggga tccgaccagc aggaggccct tggggatgcg cccccccaaa
     1861 cgggaaaact ttttcggatc ccgcaggaat tcgatgacct cctggagttc cgccttggcc
     1921 tcgtcgatcc ccgccacatc ttcgaaggtc accttgttcg ttttgtccgt catgagcctg
     1981 gcgcggcttt ttccaaaggc catggccttc cctcccccgc cctgcatctg gcgcatgaag
     2041 aatatccaga cgccgatgag aagcagcatg ggaaaccagg agatgagtat cgtcatgtac
     2101 cacggggaat cctcggcggg tttcgcggcg atgcgcacat tggcggcctt gagcttatcg
     2161 acgatcgtgg cgtccttggg catgaaagtc ttgaaatttt ttccgtccgt gagcttgccg
     2221 gtgatattgt ctccctggat ggtcacttcc cgaacctgtc cccgttccac ataggcgacg
     2281 aaatcactgt aaacaacact ttcctgttgt cccttgggct gggtaaaaag gtgatacagc
     2341 aggacgaata ccagactgat caccagccac agggcgatat ttttctgcaa ttgattcaaa
     2401 aggatgtctc ctgatacttg aattcgccgc tgaggcgatg cccctccctt acaataacct
     2461 tactctcatt tcaatggatt tctatgccgg acgccggtaa tcgatccgca agatcctctt
     2521 tgtttccgaa gtgattttga actcttcatg aacgcacaca ccggggatcc aggcgatccg
     2581 cttgtcggcc acaataaggg gcaatcgttt gcgctcgttc ctgccgatct ttccgtccac
     2641 gaagatgtcc tgcagcttct tttcgcccgc catccccaag gggcgcattc gatccccggc
     2701 gcgaaagttt cgcaccatga ccggcgtacc ggtccggtcg ccgtcgagac aggccgtagc
     2761 cgtaacctgg tcgtcggttt ccgggcggga atcgccgcaa ttcgcccggt ctttcgcgcc
     2821 cgggaggggc cttgtccgct cccggcaacc ctgacgatcc cccgcccctt cccagcgccg
     2881 gcaatcaaaa tcctcgctga tccggaatgt caggatatcc cccgtctccc gaatcgtcag
     2941 ttctccgggt gtggccaccg tataagcgaa ggcgaagtcg cggccgccgg tctccggacg
     3001 ggccaagatc agccgcccgt agtcccgctt cgcttccaga ccatggggaa ggtggagacg
     3061 tcccgacggc cgcggcgcga aggccagttc cagaaccgcc tggacatgat gaaaaaaaat
     3121 cccctgctcc acatctgtta agcgctccag cagatgtttc accagccggc gggaaagggc
     3181 gaccggctgc acccggattt tctctacttc caggctgaaa ccctccgggg taacagcgat
     3241 gttccaagcc gtcaggagac gctttacctg actcttaaag aattcgtcct cctccttaag
     3301 cacggcggcg gtccgggcca gggcacgatc gatattccgg ttgtaatccc gccggagcag
     3361 cggcagcaga tcatggcgaa tgcggtttcg caagtgacgg cggtcgtcgt tggagcgatc
     3421 cataacgcaa tccagtccct cctgagtgag gaaggccatg atttcccggc gttcaatgtg
     3481 gagaaaaggg cggatataaa ggccgtcccg cattggggtc atccccttga gcccctcgga
     3541 accgctccct cggaagagat tcatcagtac cgtctccgcc tggtcctgga gatgatgccc
     3601 cagggcgatg cggtgcgcac ctactgttgc cgccacccgt tccagaaatt cgtagcgtac
     3661 cacccgggcc atatcttccg tcgatcttcc cgccgccttt ttccgcgcct ggatatccgc
     3721 ccgctccact gtcaacggca ccgcccggcg ggaacacagc tccctgacga aacattcttc
     3781 cgcctccgct tccccacgga ggccgtgatt gagatgcgcg gcggccacgg agactccgta
     3841 ctcctcagcc agcatccgca gcaccgcaag gagggccacg gaatccggac ccccggacag
     3901 ggccacgagg accgtatcac cgtcactgag cagggcgaat tcttcgatgg ccttccggac
     3961 ctttttcaac atggcgaaac cgattaggga aatattatct cagcaaaata tatttttcag
     4021 ttccgccagg ttttcgcagg tcacatcggg tcgcagggca aggagccgct gccgttcccc
     4081 gaaaccgttg agaaaggcgc aacttataat ccccgcattt ttcgccagga ggatatcgtt
     4141 atgaccatcg ccgacgatcg ccgctttttc ccggggaata tggtaatgat cgaggtaatg
     4201 atcgacaagg cgggcatcgg gtttgaggta gggccggaaa tctccgccga tgatgtcatc
     4261 gaaataatgg gcaatgccga gacttttcac tattttaaca gtaaactcgt gtttcttgtt
     4321 gctgatcacc atctttttct ttcccgcgaa atgggccaga cattcttcca ctccgggata
     4381 cagagccgtt ttgtccagca tatgctcgcc gtaataggct ctaaagacgg tcagcgcctc
     4441 cgccagggcg taatgatgat ccgtccccag ggatctccgg atgagctcct gcaccccgtc
     4501 accgacatag ttcatgatcc gcttatgctc cagcgtagcc atacccagct tctccagggt
     4561 atgattgacg gcggcggcaa tatcctgacc ggtatccgcc agcgtgccgt cgagatcaaa
     4621 aacaaataaa tcaattttta tcatatgtta ccctattcat gcaaaattgt ccttgactgg
     4681 tatatagaac tcgggtttga atggcaagca aatcgaggaa taattttttt caaggagcat
     4741 ggatatggaa aaacgaccct ggaccaaata cggggccttc ttggtcatcg ccctgttcat
     4801 ggccgtattc gttctggaac tcgatgccta tgcccgggtg ggcggcggcc gttccttcgg
     4861 cagccgtggc tcacgatctg ccttcagcac ctccaagccg tctcccagcc agccgtcgag
     4921 tcccacccgc agtatgacca gcccatcacc ggcgtcgcca cagtcgcccc agcggggagg
     4981 attcctccgc accatgggcg caggacttgc gggaggactt ctgggcggtc tcctcttcag
     5041 cagcctcggc ttcggtcacg gcggcggttt tggcggcggc atcggccttt tcgatatcat
     5101 tctcataggc ggaatcttgt acggaatcta ctggttcctg aaacgtcgcc gtcagcaaga
     5161 agccatggcc acttcccaga cgtcctttta tcaggcctcc ggactgcatg cccaggcttt
     5221 gccaagcaat gcccaaccag tcgacgtatc atcccgggac gactatggcg ccgaccaccg
     5281 gcagggtctc acccacatcc ggttgatgga cccacaattc aacgaagata attttcagac
     5341 cctctgcatg gatatcttct ttaagatcca gggggcatgg gcaaatcggg acatggcgtc
     5401 cgcccggtct cttctcaccg acgagatgtt ccggatcctc cagagcgaag cggagcagat
     5461 gcaacgggat aaaaagttca acaagctgga aaacattgcc gtccgcagcg tggaaatcgc
     5521 tgaggcctgg caggaggaag gccgggatta tatcacggtc cgttttctgg ccaaccttct
     5581 cgattacacc gtctcggaac ggggagagtt actggacggc agcaagacgg atccagtcaa
     5641 attcgaggaa tactggacct ttgtccgccc cgtgggcaac aacccctggc agttgtccgg
     5701 catcgatcag ggcaggtaaa tgaaaccgaa agatcggata gccattgtcg ggttgggcaa
     5761 ggtgggaacc gccatgggat acctgttgaa aagggccggt tatcccatcg ccgcaatagc
     5821 cgaccgtaat cccgcggcat tggcgaaggc ccacccctat accggcggcc ggatttgccc
     5881 aaccattgcc gccgcgcccc aggacgccga tgccgtactc atcactacca ccgacgacgc
     5941 catcgcctcc gcctgtcgag agctcgccgg cgctctccac cccggccaga aggtgattca
     6001 ttgcagcggc gccggttccc tcgacctttt gatctccgcc cgggaatcgg gagccttaat
     6061 cgccagtatc catcccatcc agtccttcgc ccatgtggag ggggccatcg acaacatccc
     6121 cggcagcacc ttcgggatca ccgccggcga ggaaatcagg gagtgggccg tggatttcgt
     6181 cctcgctctg gacggggcgc cctttttcgt tcccgacacg gacaagcccc tataccacgc
     6241 cgccgcatgt atcgcctcca actatctgac caccctgctc tatatggtgg aagaaatata
     6301 tctgcacctc ggactgaacc gcgaggaggc cattcgcgcg ttctggcccc tggtccgggg
     6361 caccttgagg aatatcgagt cccggggaac cgtagaggca ttgacgggac ccattgcccg
     6421 gggagatatc gggacggtgg aaaagcacct gctcgctttt caggataagc tgcctgaatt
     6481 tctccccgcc taccgggcac tgggcataac cgccacggat ctgggactta aaaagcggag
     6541 tttgatggaa gaaaaggcaa acatgatcaa acaactactt cagggaggaa aaacatgagc
     6601 acacaggttc aaattaccag ggtgactacg gccaagattc gggacatgaa gaaaaaaggt
     6661 gaaaagatca ccatgcttac cgcctacgac tatcccacgg cggtggcaat ggattccgcc
     6721 ggcgtggata tctttctggt cggggattct ctgggtatgg tcgtactcgg ctacgacagc
     6781 acactgcccg tcaccatgga ggacatgctc caccacacca aagcggtctg tcggggaacc
     6841 cagcgggcca tggtgatcgg agacatgcca ttcatgtctt atcagacatc cgtcgccgag
     6901 ggcgtccgca atgccggccg gtttctccag gaagcgggcg cccaggcggt aaagttagaa
     6961 ggtggacggg aagtggcgga ggtcacgagc cgcatcgccg ccgccgggat tcccgtcatg
     7021 gcccatatcg gtctgacccc ccagtcggtc caccaactgg gaggctacaa ggtccagggc
     7081 aagggagaca aggcggccca gcgccttttg gaagacgcca agatcctgga agaagcgggg
     7141 gccttttcca tcgtcctcga atgcgtcccc aagggtctgg ccaaggaaat caccgccgcc
     7201 cttgaaattc ctaccatcgg catcggcgcc ggggtggatt gcgatggtca ggtcctggtc
     7261 gtccacgaca tgctgggtct cttcgagcgg ttcacgccga agttcgtgaa acaatacgcc
     7321 cgcattaacg atcacatccg ggaagcggtg gaaaaatact gcgcggaggt gaagagcggc
     7381 accttccccg acgacgccca ctcgtttatg tagcatccgg ccaaaggcat atgcaggtcg
     7441 atgagttgcg tcagccacgg tcataaatga tccggaaccc gttgttcacg ccgccccgca
     7501 gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nccccgcagt tagctgcggt
     7621 ggtgtatcca aaacggcaag gaacgaagtg tgcttcaggg aatataaccc cggtagaatg
     7681 aactaccctg cagcaagcta cggattacct aaaaccccgg ggaacgatgc aagcttcggg
     7741 gaatataacc ctgaaagatt aatattatca cgcggttgtt cgtcacggct gccgtgactt
     7801 ttttcccatt ccggctgcga tctgtttggc cctgttgaac gatgtggtat tgacgactgt
     7861 ttttcccgcc ggggtcgtag cggccagttc gtgcatctgt tgcacccgtt cctcactggg
     7921 cggatgggtg ctcatggcat cggagagact cttggaaatt ccgccgcccc ccttggaggc
     7981 gcttttttcc atcgccagga gtttttcgta aaatttcccc acctggtcct tgtcgtatcc
     8041 cgcccccaca gcgtatttga atccaaggcg gtcggcctcc atctcgtcct cccgggagtt
     8101 taccaggaag gtgtacttct gggccagcat cccgccacct gctcctaccg ccccacccag
     8161 gagggccgct ttggcatggc aggccgtgtc gtcgggaccg cagatcatcc tgcccagacc
     8221 gtagcccacg gcggcgccta aaacagcccc acccgcggcg tacatccagg tcttcttccc
     8281 ttctttttcc atggcgtaca tccgctcggc ggaatggcgg gcgacaacgt gaccgatctc
     8341 gtgggagatc accccggcaa gttccgcctc attggacgcc gcggcaatga gcggcgccgt
     8401 gacgaagatc tttcccgccg gcatggcaaa ggcgttaacg tccgtcacat ccaccacagt
     8461 gaagtcatag tgataaggat tgccctccag gttgttcgcc ttgacgatct tcatccctaa
     8521 atcggacaca tatttctgca gttccttgtt ctgcgccgga ggatagtcct tcaacatttt
     8581 cgggagggct tcctcggtga ggcgcctttc gtcctccacg gtcatgccgg tttcctgacc
     8641 tacattgctc ccttcccgat aacgggaact ctgggtggag gcgcaggaga caagcaccgc
     8701 caccaataga atcatcatac acaacaatct ttttttcatc gccatttcac ctcataaaaa
     8761 ttcgtctcga tcaattatgc ccaaagaccg ccatgaacgg ctccgatatc ggcgattcct
     8821 ccctgccgca gttcctcacg ccactaaaaa agtgcatcat tctttggcag cctcggataa
     8881 aggaaatcgt ctcaatcagc cggcctggcc gtcgatcttt acaaccggac cgtcagcccc
     8941 cggtcccgaa gatattcctt gacctggcgg atgctgaagg tccggaaatg aaagaccgac
     9001 gccgccagca ggatggaggc gcctcccttg actacggcat cgtggaaatg ctccagtttt
     9061 cccgccccac cggaagccac tacgggcacc gtcacgacat cggcgatggt ctggatgaat
     9121 tccaggtcat agcccgtgag tgccccatca ccgtccatac tcgtgggcag gatcaccccc
     9181 gcccccagtt cctgacattg tccggcccag gcgatggcat ccttacccac cggcctggtc
     9241 ccccccgcca caaccagttc gaacccggac ggcagggccg ggttgcgccg ggcgtcgatg
     9301 gccacggtga tcttctccga gccgaacttc ttcactgcct cccggaccaa ctcgggattc
     9361 cggaccgcgg cgctgttcat ggaaacggcg ttggccccgg cagccaggac cagttctata
     9421 tcgtccaggc tgtcgatgcc accgcccacc gtgaggggga tggtgatgac ggcagccacg
     9481 cttttgaccc attcgagcct ggtcttgcgg ttctctaccg tcgcggcgat gtccagcatg
     9541 gccagttcat ccgccccctc ccgttcataa aaggcggcgt tctccacagg atctccggca
     9601 tcgcggatat ccacgaagtg cacccccttg accacccg
//
LOCUS       DAOSMF010000072         9116 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_8559_length_9116_cov_25.392672, whole genome shotgun sequence.
ACCESSION   DAOSMF010000072 DAOSMF010000000
VERSION     DAOSMF010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 9116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9116
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8559_length_9116_cov_25.392672"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1577)
                     /locus_tag="PLT63_07670"
     CDS             complement(<1..1577)
                     /locus_tag="PLT63_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HOD98640.1"
                     /translation="MTLPFLFACGSRDTEAPPTAATGSGPAYGDIIVEGSIGDASNLI
                     PILSSDATSHQIGGLVYNGLVKYDRDLKIVGDLAESWKISPDGLDIVFHLRKGVKWHD
                     GEPFTARDVLFTYQVTIDPKTPTAYAGDFLKVKKVTVLDDHTLRVCYDKPFAPALMSW
                     GSPILPSHLLFGRDITTSPLSRRPVGTGPYRFREWVAGQKIVLAANPDYFEGRPYLDG
                     YVMRIIPDTATMFLELRAGGIDQMGLTPLQHTRQTENNLFRKNYRKYRYLSFSYVYMG
                     YNLKNPLFADKRIRQAIAYAVNKEEMIRGVLLGLGQAATGPYKPGTWAYNNRVRTYPY
                     DPGRAKSLLAEAGWRDSDGDGILDKDGRPFTFEIITNQGNEVRAKCAEIIQKQLADVG
                     IRVKIRILEWAAFVNDFINKRRFEATILGWTIPQDPDIYDVWHSSKTGPDELNFISYK
                     NAEVDRLIERGREVFDQQERKRCYDRIQEILAEEQPYLFLYVPDALPIISARFRGIDP
                     APQGITHNFIKWYVPKE"
     gene            1977..3566
                     /gene="cimA"
                     /locus_tag="PLT63_07675"
     CDS             1977..3566
                     /gene="cimA"
                     /locus_tag="PLT63_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HOD98641.1"
                     /translation="MKTREIMMYDTTLRDGSQGEQINFTAEEKLRIAKRLDEMGFPYI
                     EGGWPGSNPKDVRFFELAKGVKFQNARLTAFGSTRRADLRVEECPNIQALIAAETPVV
                     TVFGKTWDLHVTEILQISPEQNLAMIGETVAYLKEKGKEVIYDAEHFFDGYRRNPDYA
                     RRTVAAALTAGADWVVLCDTNGGTLPHEIEAMTAEIARLVPPGRMGIHAHNDGSLGVA
                     NSLAAVRTGATMVQGTINGYGERCGNADLIPIIANLQLKMGKICLSEEAMSHLTNLSN
                     YVADVANISPLNSRPFVGRSAFAHKGGVHVSAILKNPLAYEHIAPELVGNKQRVLVSD
                     LAGKSNIEYKARELGIDLGQNNGLSRRIVDEIKRMEDEGYQFDAAEGSLSLLIKKITG
                     EFVEPFTLECFNVIATKTAHHPSQSQATIKITVGGEVELTAAEGNGPINALDNALRKA
                     LTKFYPQLNRMHLTDFKVRIVEGSEGTAAKVNTTIESQDEDGTWTTVGVSENVIEASW
                     LALVDSLQYKLSKDRDRMNNR"
     gene            3698..4306
                     /locus_tag="PLT63_07680"
     CDS             3698..4306
                     /locus_tag="PLT63_07680"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOD98642.1"
                     /translation="MLVAINSQNPQPRLIRKAVEVLREGGIIIYPTDTVYGLGCDLFN
                     KRGIEKIYEIKKRNKKQPFSFVCADLKDISKYAFVTDYAYRTMRRLLPGPYTFVLQAS
                     RLVPKIILPKRQTTGIRVPDHPICLALVKELGQPIISTSVKGEDGETLNNPVLIRERF
                     GHCVDLIIDGGVCPGGQSTVISLEDDVVDIIRVGRGDVSAFE"
     gene            4327..6654
                     /locus_tag="PLT63_07685"
     CDS             4327..6654
                     /locus_tag="PLT63_07685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOD98643.1"
                     /translation="MGKQMLINAVHPEQKRMAIVDDGKLVDYNIQMAVKEPITGNIYK
                     GIVMKVERGLQAAFVNYGGKKDGFLPLRDVSQDYFTERREGENGPEGGHGHGRSFLKV
                     GQEVIVQVMREVSERKGALLTTYVSLPGRYLVLMPNKQGGGISRKIEDEEDRKRLKEI
                     MGQIKLDEGMGFIIRTAGMSRTKQELARDYQHLSRLWKEIQKAAASIPAPAVIYQESD
                     FGVCSLRDYFTSEIEEILVDDTETFRKMRAYCKTVSPRTVKIIKLYKDMVPLFDRYSL
                     EDQIRVIYQERVDLKSGGYLIINPTEAMITIDVNSGRGSNKRNVEETAFRTNLEAAEE
                     IARQLRLRDLGGLIVIDFIDMMDRQHQLEIERAFKKALSVDRSRIQMSKISKFGMLEL
                     SRQKKQSTIQEISYTACPHCKGTGFRPSLEYIALSAFRKIESQAVKRIYSGLKVSLPH
                     PVSDYLLNQKRLEISRLESMCEMSIHISGHADMPWDEVRMEYEAREGAPEGVTEKDKS
                     AETAKEHHPGGAGKPREREAPRGVRSGVAGKETEGAAAGPAAEAAAAPGETPKKKSRR
                     RSRHRRKKSAADGASSAVNKVTETGETETTATTAPAAVMETDAGTFGEPSNIDDDAGQ
                     GVAEDSPFMASEVAPAMEAKEGTDGSGIAAVVGSEVVTAEMPEDVRKIEKPPPKRTRS
                     RKTGKVALAVETTPASDGPLPVETTGDLPEKEGETAKAPRKKRRSPGRTSQRRKPAAA
                     ASETPETGGETPPGKEVPAEFPDALDEVPPEAAGP"
     gene            complement(6813..7625)
                     /gene="proC"
                     /locus_tag="PLT63_07690"
     CDS             complement(6813..7625)
                     /gene="proC"
                     /locus_tag="PLT63_07690"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HOD98644.1"
                     /translation="MLKGKKIGVIGGGKMGGALIGGILAHHLVPAEAVIVADVDKGQR
                     EELEKNHAVKTTDDNRLAAKSAQIIILAVKPQNMAEVLAGISGVVDKNKLVISIAAGI
                     PIRFISERLKAGARIIRTMPNTPALIGEGMAALAAGAEATGEDMDTALHIFNAVGKTV
                     VVKEEQLDAVTGLSGSGPAYAFIMIEALADGGVHMGLSRDTALTLAAQTLQGAARLFL
                     SGERHPGQLKDMVTSPGGTTIAGIRALEDGKLRGTLMAAVEAATLRSKALGS"
     gene            7874..8647
                     /locus_tag="PLT63_07695"
     CDS             7874..8647
                     /locus_tag="PLT63_07695"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOD98645.1"
                     /translation="MIAKREIDGIQIAGWVSGERWRDGIRHLVFIHGSGGDHTHWAYQ
                     YGDLKNDFNVLAVDLPGHGQSAGKGERSVGAYVEWMRKILAGFEVEKPILIGHSLGAA
                     ISLSFAVQYGELPAGVVPVGGGVTMWVNPAILAGLLEDPLPIIEMAAKLSLAKQNREK
                     FAKILSAGFAKANPVVMQGDFSACNDLNLTEEVKKIKIPTLVVCGTEDKMMPPEHSQY
                     LKDNIPCARMVLIENAGHLVMMENPEAFNQHIREFAGSL"
     gene            8671..8970
                     /locus_tag="PLT63_07700"
     CDS             8671..8970
                     /locus_tag="PLT63_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005020590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HOD98646.1"
                     /translation="MFVMGNFIVALAKIIDLVLSVYVWIIIFRAVISWVNADPYNPIV
                     NFLYRATEPVLAPIRRRLPFGGSGIDVSPIIVILIVYFLQFFLVKTMIQAAVYFH"
BASE COUNT         2025 a         2550 c         2773 g         1768 t
ORIGIN      
        1 tcttccttcg gaacgtacca cttgatgaaa ttgtgggtga tcccctgggg ggccggatcg
       61 atgccccgga accgggcgct gatgatgggc agggcgtcgg ggacgtacag gaagaggtag
      121 ggctgctcct cggccaggat ctcctggatc cgatcgtagc accgcttccg ttcctgctgg
      181 tcgaaaacct cccggccccg ttcgatcagt cggtccacct cggcgttttt ataggagata
      241 aagttcagct catcgggtcc ggtcttgctg gaatgccaga catcatagat gtcgggatcc
      301 tgggggattg tccagccgag aatcgtcgcc tcgaagcgcc gcttgttgat gaaatcgttt
      361 acgaaggccg cccattccag gatccggatc tttaccctga tacccacatc ggccagttgt
      421 ttctggatga tctcggcgca tttcgcccgt acttcgttgc cctggttggt gatgatttcg
      481 aaggtgaagg gtctgccgtc cttgtccagg atgccgtcgc cgtcactgtc tctccagccc
      541 gcctccgcca ggagagattt cgcccgcccg ggatcgtaag gataagtccg cacccggttg
      601 ttgtaggccc aggtgccggg cttgtaaggt cccgttgctg cctgccccag gcccagcagc
      661 accccccgga tcatctcctc cttgttgacg gcgtaagcga tggcctgccg gatgcgctta
      721 tcggcgaaga gcggattctt cagattgtag cccatataga cgtaggaaaa ggaaagataa
      781 cggtatttgc ggtaattctt tcggaacagg ttgttttccg tttgccgggt gtgctggaga
      841 ggtgtcagtc ccatctggtc gatgccgccg gcccgcaatt cgagaaacat cgtggccgtg
      901 tcggggatga tccgcatgac gtatccgtcg aggtagggcc ggccctcaaa gtaatcggga
      961 ttggcggcca ggacgatctt ttgtcccgcc acccattccc ggaagcggta ggggcccgtg
     1021 ccgacgggcc gccgggaaag gggcgaagtg gtgatgtccc ggccgaacag aagatgactg
     1081 ggcaggatgg gggaccccca gctcatgagt gccggggcga agggcttgtc gtaacaaacg
     1141 cggagggtgt gatcgtccag gaccgtaacc ttctttacct tcaggaaatc cccggcgtaa
     1201 gcggtgggcg tcttgggatc gatggtgacc tgataggtaa acaggacgtc ccgggcggtg
     1261 aagggctctc cgtcgtgcca tttcacccct ttgcggaggt ggaagacgat gtcgaggccg
     1321 tcgggcgata tcttccatga ttcggccaga tcaccgacaa tcttcagatc ccggtcgtat
     1381 ttcaccaggc cgttgtaaac cagaccacca atctggtgcg aggtggcgtc gctggacagg
     1441 atggggatga ggttgctggc atcgccgatg gaaccctcga cgatgatgtc tccgtatgcc
     1501 gggcccgagc cggtggcggc ggtcgggggc gcctcggtat ccctgcttcc gcaggcaaac
     1561 agaaacggta atgtcattat gagggatatc gccatcagtc ggcgaaagaa tgaccggccc
     1621 gcactccttc cccccatatt ttttacgggt gttcctcccg ataaatgctt atgaatatgc
     1681 gcaaacatag cgccgccgat cctaatcgat gtggcccaca tttgcaatgg agaagatcgc
     1741 catatttgtg ttgacaaaag atccggaacg gattaatgag aacaagcgaa taagaaattt
     1801 accatgagcg gcatgaaact gaacacgatc atcggcatta ttattatcgg cataattagg
     1861 ccggacaata ccggggcctg atcgtggttt agtgtttaga taagaaataa agcaactcac
     1921 aaaatccgtt atcaggtccc ctggtagcgg attttttttc gggaagaaag gaaattatga
     1981 agacgcggga gataatgatg tacgatacca ccctccggga tggttcgcag ggagaacaga
     2041 tcaatttcac cgccgaggaa aagctgcgca ttgccaagcg gttggatgag atggggttcc
     2101 cctatatcga aggaggctgg ccgggttcca accccaagga cgtgcgtttt ttcgaactgg
     2161 ccaagggtgt gaagttccag aacgcccgac tgacggcctt cggcagcacc cgccgtgccg
     2221 atctgcgggt ggaggaatgt cccaacatcc aggccctgat tgccgcggaa accccggtag
     2281 tgaccgtttt cggcaagacc tgggatctcc atgtcacgga gatcctgcaa atttctccgg
     2341 aacagaacct ggccatgatc ggggaaaccg tcgcctacct caaagagaag ggaaaagaag
     2401 tgatttacga cgccgagcat ttcttcgacg gctaccggcg gaatcccgat tacgcccggc
     2461 ggaccgtagc cgcggcctta acggcgggtg ccgactgggt cgttctgtgc gacaccaacg
     2521 gcgggacgct gccccatgaa atcgaggcca tgacggcgga aatcgcccgc ctcgtgccgc
     2581 cggggagaat gggtatccat gcccacaacg acgggagcct gggagtggcc aactccctgg
     2641 cggcggtccg gaccggcgcc acgatggtcc aggggaccat caacggctac ggggagcgtt
     2701 gcggcaatgc cgatctgatt cccatcatcg ccaatctcca gttgaaaatg gggaagatct
     2761 gcctctccga agaggcgatg agtcacctga cgaacctgtc caattacgtg gccgacgtgg
     2821 ccaacatctc ccccctgaat tcccggccct ttgtcggccg cagcgccttt gcccacaaag
     2881 ggggggtgca tgtcagtgcc atcctaaaga atcccctggc ctacgagcat atcgcccccg
     2941 aactggtggg aaacaagcag cgggtcctag tctcggatct ggcggggaag agcaacatcg
     3001 aatacaaggc ccgggaactg ggcatcgatc tgggacagaa caacgggttg agccggcgga
     3061 tcgtggacga gatcaagcgc atggaagacg aggggtatca attcgatgcg gcggaagggt
     3121 cgctgtcgct gctgatcaag aaaatcaccg gggagtttgt ggaacccttc accctggaat
     3181 gcttcaatgt cattgccacc aagacggccc atcacccgtc ccagtcccag gctacgatca
     3241 agatcaccgt gggcggcgag gtggaactga cggcggcgga gggcaacggt cccatcaacg
     3301 ccctggacaa cgccctacgg aaggccctga ccaaattcta tccccagttg aaccggatgc
     3361 acctcaccga cttcaaggtc cggatcgtcg aggggagcga aggaacggcg gccaaggtca
     3421 acacgaccat cgaatcccag gacgaagacg gtacctggac caccgtcggc gtttcggaaa
     3481 acgtcatcga ggccagttgg ctggccctgg tggacagcct ccagtacaag ctcagcaagg
     3541 accgggacag aatgaataac cggtgatgcc gtccagttcg ttttcattgg aaaaatgaga
     3601 gaatcctccc ccgacgcggt gatgatattc cattgctaat ctccaccgtt tctgatacag
     3661 gacgcgctgt tacggaataa cggttcgaga aaagaagatg ctggtcgcca tcaacagcca
     3721 gaatccccag ccgcgcctga tcaggaaggc cgtggaagta ctccgggaag gggggatcat
     3781 tatctacccc accgatacgg tctatggttt gggttgcgac ctcttcaata aaagaggcat
     3841 agaaaagata tacgaaatca agaaacgaaa caagaaacag cccttcagct ttgtctgcgc
     3901 cgatctcaag gacatcagca aatacgcctt cgtaaccgat tacgcctacc gcaccatgag
     3961 acgcctgttg ccgggacctt ataccttcgt cctgcaggcc tcgcggctcg ttcccaagat
     4021 catcctgccc aagcggcaga cgacgggcat ccgcgtaccg gatcatccca tctgtctggc
     4081 cttggtcaaa gaactgggac agccgatcat cagcaccagc gtcaagggtg aagacgggga
     4141 gaccctgaac aacccggtcc tgatccggga acgcttcggg cactgcgtcg atctgattat
     4201 tgacggcggc gtctgtccgg gaggacagtc cacggtgatc agtctggaag acgatgtcgt
     4261 ggatatcatc cgtgtcggac ggggcgatgt ctcggccttc gaatagaaca tttacaggta
     4321 aaaaacatgg gaaaacaaat gcttatcaac gcggtacatc cggaacagaa acggatggcc
     4381 atcgtcgatg acggcaaact agtggattac aatatccaga tggccgtcaa ggagcccata
     4441 acgggcaaca tctacaaagg tatcgtcatg aaggtcgaac gaggtctcca ggcggccttc
     4501 gtcaactatg ggggaaagaa ggacggtttt cttccccttc gggacgtgag ccaggattat
     4561 ttcaccgagc ggcgggaagg agaaaacggg ccggaaggcg gtcacggcca cggccggtcg
     4621 tttttgaagg tgggacagga agttatcgtc caggtcatgc gggaggtgag cgagcgcaaa
     4681 ggggccttgc ttaccacgta cgtctccctg ccggggcgtt accttgtcct gatgccgaac
     4741 aagcagggcg gcggcatttc ccggaagatc gaggacgagg aggaccggaa acgcctcaag
     4801 gaaattatgg gccagatcaa gctggatgag gggatgggat tcatcatccg cacggcggga
     4861 atgagtcgga ccaagcagga gctggccagg gactatcagc acctctcccg gctctggaaa
     4921 gagattcaga aggccgcggc atctattcca gcgccggcgg tcatctatca ggagagcgat
     4981 ttcggcgtct gttccctccg ggattatttc acctcggaaa tcgaggagat ccttgtggac
     5041 gatacggaga ccttccggaa gatgcgggcc tattgcaaaa ccgtctcacc ccgcacggtg
     5101 aagatcatca agctctacaa ggatatggtt ccccttttcg accggtacag cctcgaagat
     5161 cagatccggg tcatttatca ggaacgggtg gatctcaaat ccgggggcta tctgatcatc
     5221 aatcccacgg aggcgatgat taccatcgac gtcaattccg gccgggggtc gaataagcgt
     5281 aatgtggagg aaaccgcctt ccgcaccaac ctcgaggccg cggaggaaat cgcccgccag
     5341 ttgcgtctcc gggacctggg gggccttatc gtcatcgact tcatcgacat gatggaccgc
     5401 cagcatcagc tcgagatcga gcgggccttc aaaaaggccc tgagcgtgga ccgttcccgc
     5461 attcaaatgt ccaagatctc gaaattcggc atgctggagc tttccaggca gaagaaacaa
     5521 tcgacgattc aggagataag ctacaccgcc tgcccccact gtaagggcac cggtttccga
     5581 ccgtctctgg aatatatagc tttgagcgcc ttccggaaaa tagagtccca ggccgtgaag
     5641 agaatctact cggggctgaa ggtaagcctg ccccatcccg tgtccgatta tcttttgaac
     5701 cagaagcgtc tggagataag caggctcgaa tccatgtgcg agatgtcgat ccatatttcc
     5761 gggcatgcgg acatgccgtg ggacgaggtc cggatggagt acgaggcccg ggagggggcg
     5821 ccggagggcg tcacggagaa ggataaaagc gccgagacgg ccaaggagca ccatcccggc
     5881 ggcgcgggga aaccccggga gcgggaggcc ccccgtgggg ttagaagcgg ggtcgcgggg
     5941 aaggagaccg agggggcagc ggcaggaccg gcggcggaag cggcggccgc cccaggtgaa
     6001 acgccgaaga agaaatcacg ccggcgctcc cggcaccgcc ggaaaaaatc cgccgccgat
     6061 ggggcgtcct cggccgtgaa taaggtgacg gagaccggcg aaacggagac cacggcgacc
     6121 acggcgccgg cggcggtcat ggagacggat gccggaacat tcggagaacc atccaacatc
     6181 gatgacgatg ctggccaagg ggtggcggaa gattcgccct tcatggcttc ggaggtggcg
     6241 ccggcaatgg aagcgaagga aggtaccgac ggatcgggaa tcgcagcggt agtggggagt
     6301 gaggtagtaa ccgcagagat gccggaggat gtcagaaaga tcgaaaagcc gccgcccaaa
     6361 aggacgcggt cgcgtaaaac agggaaggtc gcccttgccg tggaaaccac gccggcatcg
     6421 gatggccctt tacccgtgga gacgaccggt gacctgccgg aaaaggaagg ggagaccgct
     6481 aaggcgcccc ggaaaaaaag acggagcccg ggccgtacct cccagcgccg aaagccggca
     6541 gccgcagctt ccgaaactcc ggaaaccggc ggtgaaacac cgccgggtaa ggaagtccct
     6601 gccgaatttc cggatgccct agacgaagta ccgccggaag cggcaggccc ctgagcacca
     6661 taccggcaga accgcctgtt cagtgatgcc gacttcactc acggccattt ttcctcccct
     6721 ccgtcgtaat gggagcgggc tgactcataa gagtatatag ttgaatatat tgattaaaaa
     6781 atctcctccc cttcaagggg aggattggcg attcagcttc ccagggcctt ggaacgcagg
     6841 gtcgccgctt ccaccgcggc catgagggtg ccccggagct ttccgtcctc caaggctctg
     6901 attccggcga ttgtcgttcc tcccggcgag gtcaccatgt ctttcaactg gccgggatgc
     6961 ctttccccgg aaagaaagag ccgtgccgcg ccctgcaggg tctgggcggc cagggtgagg
     7021 gccgtgtcgc gactgagtcc catatgcacc ccgccgtcgg cgagggcctc gatcatgatg
     7081 aaggcatagg cgggcccgct gccgctcaag ccggtcaccg cgtccagttg ctcttccttg
     7141 acgacgacgg tttttcccac ggcgttgaaa atatggaggg ccgtatccat gtcctccccg
     7201 gttgcttccg ctccggcagc aagtgcggcc attccttccc cgatcagggc gggggtgttg
     7261 ggcatggtgc ggatgatcct cgcccccgct ttgagtcgct cagagatgaa ccggatggga
     7321 atgccggcgg cgatggaaat gacgagcttg tttttgtcca caaccccgga gatgccggcc
     7381 aggacctcgg ccatgttctg cggtttcacg gccaggatga ttatctgagc cgatttggcg
     7441 gccagtcggt tgtcgtccgt ggtctttacc gcgtgatttt tttccagctc ctcacgctgc
     7501 cctttatcca catcggcaac gatcaccgcc tccgcgggaa ccagatgatg ggcgaggatg
     7561 ccgccgatga gggccccccc cattttaccg cccccgatca ccccgatttt tttccctttc
     7621 aacataacgt ctcccccctt gattgaataa tgaacgtgcg aatctgtaat cctttttttg
     7681 ttccggtgtc aatagattga tgggaaaaat acccggttgc ggaagataag gaagatgtgt
     7741 tacgaaaagt acaccggtgg gtctgggaaa aacggccgcg ccggccgggg gaacataacc
     7801 ggcttttttc atcacggcgg cagcagcgct tgggccgggc atcccgaaat agtcagacgc
     7861 cggggaggtg gaaatgatag cgaaaaggga aatcgacggc atccagatcg ccgggtgggt
     7921 aagcggcgag cgatggcggg acggcatccg ccatctcgtc ttcattcatg gctccggggg
     7981 agatcacacc cactgggctt atcagtacgg cgacctgaaa aacgatttca acgtactggc
     8041 cgtggatctg cccggacacg ggcagtcggc ggggaagggg gagcggagcg tcggcgccta
     8101 cgtggaatgg atgcgaaaga tcctggccgg tttcgaggtc gagaaaccga tcctcatcgg
     8161 ccactccctg ggagccgcca tcagtctatc cttcgccgtt caatacgggg aactgccggc
     8221 gggcgtcgtt cccgtcggtg gcggggttac catgtgggtc aatccggcaa tcctcgccgg
     8281 tctcctggaa gacccgctgc ccatcatcga aatggcggct aagctttccc tggccaagca
     8341 gaaccgggaa aagttcgcga agatcctctc ggcgggtttt gccaaggcga atccggtggt
     8401 catgcaaggt gatttctcgg cctgcaacga tttgaacctg accgaagagg tgaagaaaat
     8461 caagatcccc acgctggttg tctgcggaac ggaagacaag atgatgccgc cggagcattc
     8521 ccaatatctc aaggataata tcccgtgcgc ccggatggtg ttgatcgaaa atgccggaca
     8581 tctggtcatg atggaaaacc ccgaggcatt caaccagcac atccgggaat ttgccgggtc
     8641 tctgtagagc cgccacaaga ggaagccgcc atgttcgtaa tgggtaattt tatcgtcgct
     8701 ctcgccaaga tcatcgatct ggtcctgagc gtctatgtct ggataatcat cttccgggcc
     8761 gtcatctcgt gggtcaatgc cgatccatac aaccctatag taaattttct ataccgggcc
     8821 acggagccgg tgctggcgcc cattcgccgc cggctgccct tcggcggttc ggggatcgac
     8881 gtctcgccca tcatcgtcat cctgattgtg tacttcctcc agtttttcct cgtcaagacg
     8941 atgatccagg cggctgttta cttccactga tgattcggga aaccccggaa ggttttttgc
     9001 tgaacgtcaa ggcgctgccc cgttcctccc ggtgcgggat ctgcggcatc cacggcgacg
     9061 ccttgaagat caaggtgacg gcgccccccg tggacgggcg ggcgaacgag gagatc
//
LOCUS       DAOSMF010000073         9101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_8577_length_9101_cov_19.956887, whole genome shotgun sequence.
ACCESSION   DAOSMF010000073 DAOSMF010000000
VERSION     DAOSMF010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 9101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9101
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8577_length_9101_cov_19.956887"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="PLT63_07705"
     CDS             complement(<1..364)
                     /locus_tag="PLT63_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOD98647.1"
                     /translation="MTAETAYSDLEVYLARRKAVVEEALDRFLSPETGVPERIMAAAR
                     YSLFAGGKRLRPILCLAAAEAVSGAWSDALPCACALEMIHTYSLIHDDLPAMDDDDYR
                     RGRPTNHIVFGEGLAILAG"
     gene            complement(367..594)
                     /locus_tag="PLT63_07710"
     CDS             complement(367..594)
                     /locus_tag="PLT63_07710"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOD98648.1"
                     /translation="MAKEKFEEALKKLEALVKTMESGDLTLDDSLKAFEEGIRLIRFC
                     TRKLDEAERKVEILLRDDDDRPGAVPFPPEG"
     gene            complement(770..2143)
                     /gene="xseA"
                     /locus_tag="PLT63_07715"
     CDS             complement(770..2143)
                     /gene="xseA"
                     /locus_tag="PLT63_07715"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOD98649.1"
                     /translation="MQEVWTVSELNERIKVLLESSFEFLWVEGEVSNLRRPASGHSYF
                     TLKDQRSQIRAVLFRTASKPPFVLEDGMQIACRARLTAYTPRGEYQLIVERVEPRGLG
                     ALQMAFEQLKDKLQTEGLFDARHKKPFPFLPRGIGVITSPTGAVIRDILQITGRRFPA
                     VPVLIAPVRVQGPEAAPEIVRALALLNWESGESGAYPDLEVIIVARGGGSLEDLYPFN
                     TEEVARAVFRSRLPVVSAVGHETDYTITDFVADLRAPTPSAAAEMVVPDRDDLRRQVG
                     DAYGRLLQARQRRADRWRERIGAAAGRLKSPDRILADCRLLLDDHRDRLCLALNRHIL
                     SRRHDLQYLRTRLDHVCPTGTIDRIRVVLVHRREQFAAGIGAFFRRQRAVMDRQGVSL
                     QALSPLEVLRRGYSITRRFPDGPIVKRAADLVTGDRLSVRLAAGEIRARVELVDTEEK
                     KEGGSLF"
     gene            2275..3294
                     /locus_tag="PLT63_07720"
     CDS             2275..3294
                     /locus_tag="PLT63_07720"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: protein motif:HMM:NF002699.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HOD98650.1"
                     /translation="MKKKDLLKKPVHHIDITSYDTTDLVEAMQKMSFSARDLGEAAAI
                     YDRMLKDRKCAIFLTIAGSTSAAGCMQIYVDLVRHNMVDAIVATGATIVDMDFFEALG
                     FRHYQGSPHADDRVLRSLYIDRIYDTYIDEEELQLCDRTIREIADALPPRPYSSREFI
                     RETGKYLTVNAKKKDSLIQVAYENDVPIFCPAFSDSSAGFGLVLHQHHRPECHVTIDS
                     VKDFRELTMIKIAAETTGLLMIGGGVPKNFAQDTVICAEILGKDVPMHKYAIQITVAD
                     VRDGACSSSTLKEAQSWGKVDVTFEKMVFAEATTVLPLLANYAYRKGGWKKRKHWKFT
                     NIFPG"
     gene            complement(3352..5607)
                     /locus_tag="PLT63_07725"
     CDS             complement(3352..5607)
                     /locus_tag="PLT63_07725"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOD98651.1"
                     /translation="MISREQTEVRDLRHDLDKFIGEQPAEAKKVQEKIRDLNRELRHV
                     LLVHGISENTPMECRYTLKKMDVLKESVRRGIDPIKNQIKMIDQFEKETQGRKEEYVK
                     LAEDVSLAASVPIIRYYVGELDGLLSSTVKARQLLAFFPNIAEDFLLRLEQRRATVEK
                     RLIDLWKTYFFKPLPSSYFTAEAWHWTSIVAVNWWKFAPLFGLTPGEEQKSAFLDFIT
                     QLLGFWLAVSFGGYFFLAWLGKKYPASRPVRHFLPALIWVTLGLSILVNTEISEHFLF
                     GTYQSFGEIFLTGGAVSLAWNLRKTISTAVEPRSGYNILWPLWFIYAVGVFVQVLRIP
                     MVVTTLVFVSLFLLSILYYYLLGKRITNARDRKLAVLTTVLMAVLVLTALLGWGNLTM
                     LVSTFCFMIVLNIELGASLSGYFWRIIHSGRLKSHLLIMLSNMILPLIFIGLLALMVI
                     WASLYMGGMPLLQRIVAWEIDWGVLRLRITTLLTLTALFFLTRSGIAFSHTAIQFQRS
                     KWENIEEGAVKSLQTLSSYILWSCYVIVSLHFLGVGISNLTIIAGGLSVGVGIALQDL
                     LKNFVSGLMLLFGRSIHPGDEIQVDDVRGSVKKINIRSTVVQTNEDSTIFLPNADLVA
                     KKIVNWTHKDPRGRAEIVVGVAYGTDMDLVRELLIRCALTNPDVLKTPPPYVLFSDFG
                     DNALILHLRFWIMHVVLATDRVKSAIRFDIDRVFRENNIEIAYPQQDIHIRSLAGWSG
                     CLEKPPAPPPA"
     gene            complement(6060..6135)
                     /locus_tag="PLT63_07730"
     tRNA            complement(6060..6135)
                     /locus_tag="PLT63_07730"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6100..6102),aa:Ala,seq:cgc)
     gene            6337..7419
                     /locus_tag="PLT63_07735"
     CDS             6337..7419
                     /locus_tag="PLT63_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HOD98652.1"
                     /translation="MAKIDAFFQLMNEQGASDLHLVTGQQPAIRIRGEIERIKYDLLD
                     NDGLKAMLYEIAPEHKIKQFEETGDVDFAYEIPGLARYRANFFMQKWGCAAVFREIPS
                     NILTAEQLGLPAVVSKLASLPRGLVLVTGPTGSGKSTTLAAIIDQANRTRKDHIITVE
                     DPIEFVHKSQQCIVNHREVGVHTKSFSSALRGALREDPDIILVGEMRDLETIRLAVEA
                     ASTGHLVFGTLHTTSAAKTVDRVIEVFPASEQAQIRSTLADGLRAVVAQVLFKRIDKK
                     GRCAALEILIATSAVRNLIREAKTFQIPSMIQTGKKYGMQLLDDAIYELVNKGWIDPD
                     EAYAKANDKTRFRPLLKKPPADFTDA"
     gene            7580..8758
                     /locus_tag="PLT63_07740"
     CDS             7580..8758
                     /locus_tag="PLT63_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HOD98653.1"
                     /translation="MRKQEIDFILGKMLDSHGNVSDLNITVGKPFQVEASGQLTGVEL
                     DPPFSKLTPFQSEIFAMNLINQDHRLTETLIAEGSCDSSYELPGKARFRVNIFSQRGN
                     YSIVLRKLETRIPTCEDLNLPKAFYKMAEEKNGIILVTGATGSGKSTSLAAVLDKIND
                     TKSVHIVTLEDPVEFSHPHKKATFNQREMGNDFDTFASGLRAALRQAPKVILVGEMRD
                     RETVEIGLSAAETGHLVVSTLHTVDAGQTINRILGMFSTEEETQIRIRLADTVRWIVC
                     QRLLPKVGGGRVAAFEIMGSNLRIKDTILHGESEGKTFYEIIQASKAFGMITFDDYIV
                     ELYEKGLITEETARAYASNKGIVGRGIDSVKSTKGEATTELGKLSIDKDYGKPKPKWP
                     "
     gene            8864..>9101
                     /locus_tag="PLT63_07745"
     CDS             8864..>9101
                     /locus_tag="PLT63_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98654.1"
                     /translation="MEEQKTLAKDVAAADYNASDMPFDFVEEGEQTALICEADGSVRE
                     KIVAAMKATGYRTSEAANARDALKKTRFHVYDVVV"
BASE COUNT         2075 a         2684 c         2511 g         1831 t
ORIGIN      
        1 cccccgccag gatcgccagc ccctcgccga aaacgatatg attcgtcggc cggccccgcc
       61 ggtaatcgtc atcgtccatg gccggcaggt cgtcgtgaat cagggagtag gtatggatca
      121 tctccagggc gcaggcgcag ggcagggcgt ccgaccaggc gccgcttacc gcctcggcgg
      181 cggccagaca gaggatgggg cggagccgct tgccgcccgc aaagaggctg taacgggcgg
      241 cggccattat ccgttccggt acgccggtct ccggggagag aaaacggtcc agggcctcct
      301 ctaccacggc cttgcgccgg gccagataga cctccaaatc gctgtatgcc gtttccgccg
      361 tcattatcag ccctccggag ggaaggggac ggcgccgggg cggtcgtcgt cgtcccggag
      421 caggatctcc acctttcttt ccgcctcgtc gagtttccgg gtgcaaaaac ggatcagccg
      481 gatcccctcc tcgaaggcct tgagggaatc atccagggta agatcgccgg attccatggt
      541 ttttaccagc gcttctaact tcttcagggc ctcttcaaac ttttcttttg ccatgttcac
      601 ctctcccgcc gctcaccgca cacctgcggc ccggtccttt ccatccgttt tcccatgcgc
      661 agtcctgaaa attaataatt gcttcgtaaa acataatccc tatcatcttc cttcccggtt
      721 acgcccccct tgcccgccgg cacggaaaaa atttgatcgg caacggggat caaaaaagcg
      781 atccgccctc cttcttctcc tcggtatcca ccagttccac ccgggcgcgt atttcccctg
      841 ccgccaaacg aacggaaagt cgatctccgg tgacgaggtc ggcggccctt ttcacgatcg
      901 ggccgtcggg gaaccggcgc gtgatgctgt aaccacgccg caggacctcc agggggctta
      961 gggcctgaag gctgacgccc tgccggtcca tgacggcccg ctgccgacgg aaaaaggcgc
     1021 cgatcccggc ggcaaactgt tcccgccgat ggacaaggac cacccggatg cggtcgatgg
     1081 ttccggtcgg gcagacgtga tccaaacggg tccgcagata ctgcaagtca tggcgccggg
     1141 aaagaatgtg acggttcagg gccaggcaaa gccggtcccg atgatcgtcc aggaggagtc
     1201 ggcaatcagc gaggatgcgg tccggtgact tgagccgtcc cgccgccgcg ccgatccgct
     1261 cccgccatcg atccgcccgg cgctgccggg cctggagcag acgcccatat gcatcgccga
     1321 cctgccggcg caggtcatca cggtcgggga ccaccatttc cgccgccgcc gagggggtag
     1381 gcgcccgcag gtcggcgacg aaatccgtga tggtataatc cgtctcatgt cccacggcgg
     1441 aaactacggg gaggcgggac cggaataccg cccgggccac ctcttccgta ttgaaggggt
     1501 ataggtcctc cagggaaccg ccgccccggg cgacgatgat gacctcgaga tccggataag
     1561 caccggactc cccactttcc cagttgagca gggcgagggc ccggacgatc tccggcgccg
     1621 cctcgggtcc ctggacccgc accggggcga tcagaacggg aacggcaggg aaccgccgtc
     1681 ccgtaatttg caggatgtcc ctgatcaccg ccccggtggg cgaggtaatg acgccgatgc
     1741 cccggggcag aaaggggaac ggttttttat gccgggcatc gaaaagtccc tccgtctgga
     1801 gcttgtcctt gagttgttcg aaggccatct ggagggcccc cagtcccctg ggttccaccc
     1861 gttcgacgat gagttgatac tctccacggg gggtataggc ggtcaggcgg gcccggcagg
     1921 cgatctgcat accgtcttcc aggacgaagg gcggctttga cgccgtccgg aacagcaccg
     1981 cccggatctg gctgcgctgg tctttcaggg tgaaatagct gtgtccggag gccggacggc
     2041 gaagattaga aacttctccc tccacccaga ggaactcgaa agatgattcc aaaaggacct
     2101 ttatccgttc gtttagttcg cttaccgtcc agacttcctg catccgccga cccacctttg
     2161 tcgaaggagg cgtatcagaa atgggatagg atgacaagat ggtttttcat attggattga
     2221 catttatcct tctatgattt aaggaaaata atcactttta tcactgagga ggatatgaag
     2281 aaaaaggacc ttttaaagaa gccggtgcac catatcgaca tcacctcgta cgacacgacg
     2341 gaccttgtgg aggccatgca gaagatgtcc ttttccgccc gggacttggg cgaagccgcc
     2401 gccatctacg accgaatgct gaaggacagg aagtgcgcca tatttctcac cattgccggc
     2461 agcacctccg ccgcagggtg tatgcagatc tacgtggatc tcgtccgcca caacatggtg
     2521 gacgccatcg tggccaccgg tgcgaccatc gtggatatgg actttttcga ggccttgggc
     2581 ttccgccact accagggatc gccccatgcc gacgatcggg tcctccgctc cctctatatc
     2641 gaccggatct acgacaccta tatcgatgag gaggaattgc agctctgcga ccggacgatc
     2701 cgggagatcg ccgacgccct tcccccccgg ccctactcct cccgggaatt catccgggag
     2761 acgggcaaat acctgaccgt aaatgccaaa aagaaggatt ccctcattca ggtcgcttat
     2821 gaaaacgacg tccccatctt ttgtcctgcc ttctccgaca gtagcgccgg cttcggcctg
     2881 gtgctccacc agcaccatcg cccagaatgc cacgtaacca tcgattcggt caaggatttc
     2941 cgggagttga ctatgatcaa aatcgccgcg gagaccacgg ggctcctcat gatcggcggc
     3001 ggcgttccca agaacttcgc gcaggacacg gtgatctgcg ccgaaatcct cggcaaggat
     3061 gtccccatgc acaagtatgc catccagatt accgtggcgg acgtccggga cggtgcctgc
     3121 tccagttcca ccctcaagga ggcccagtcc tggggcaagg tggatgttac cttcgaaaag
     3181 atggtcttcg ccgaggccac gacggtccta ccgctgctgg ccaactacgc ctaccggaag
     3241 ggaggctgga agaagcgcaa acactggaaa tttaccaata tatttcctgg ataggcttac
     3301 ccgggaggtg agaaaggcta tttacactta ccgcctccat cggcggcagc gctaagctgg
     3361 cggcggcgcc gggggttttt ccaggcaacc gctccatccg gccagagaac ggatgtggat
     3421 gtcctgctgc gggtatgcta tttcgatatt gttctccctg aaaacccggt cgatatcgaa
     3481 ccggatcgct gattttaccc tgtccgtggc cagcactaca tgcatgatcc agaagcggag
     3541 gtgcaagata agcgcattgt ctccgaagtc gctgaacagg acataaggag gaggggtttt
     3601 cagcacatcc ggattggtca aggcgcatcg gatgaggagt tcccggacta ggtccatgtc
     3661 ggtgccgtag gcgaccccca cgacaatttc ggcccttccc cggggatcct tgtgggtcca
     3721 attgacgatc tttttcgcca ccagatcggc attcggcaga aagatggtcg aatcttcgtt
     3781 ggtttgcacc acggtactgc ggatgttgat cttttttacg ctgccgcgga cgtcatcgac
     3841 ctgtatctcg tcaccgggat ggatagagcg cccgaaaagg agcatcaggc cgctgacgaa
     3901 attcttgagc agatcctgca acgcgatgcc gacacccacg gatagtccac cggcaatgat
     3961 cgtcaggttg ctgatcccca ccccgagaaa gtgcagggag acgatcacat agcacgacca
     4021 gaggatataa gaagacagtg tttgcaggga cttgacggcg ccctcttcga tattttccca
     4081 tttgcttctc tggaactgga tggccgtatg ggaaaaggca atcccggaac gggtcaggaa
     4141 aaataatgcc gtcaaggtca agagggtggt gatccggagc cgtagtaccc cccagtcgat
     4201 ttcccaggca acaattcgct gcaacagcgg catccctccc atgtacagag atgcccagat
     4261 gaccatcagg gccaaaagcc cgataaagat caacggtagg atcatgttgc ttaacataat
     4321 gagcaggtgg gatttaagcc ggccggagtg gattatcctc cagaagtagc cgctcaggct
     4381 ggcgcccagc tcgatattca ggacgatcat gaagcagaaa gtggagacaa gcatcgtcag
     4441 gtttccccaa ccaagcaagg ccgtcaggac caggaccgcc attaggaccg ttgttaagac
     4501 ggccaatttc cggtcccggg cattggtgat tcgttttccc agaagatagt aataaagaat
     4561 ggagagaaga aacagggaaa caaataccag tgtcgtcacc accatgggta tgcggagtac
     4621 ctgaacaaat accccaacgg catagatgaa ccacagaggc cagaggatgt tgtagccgct
     4681 ccgcggctcc acggcggtag atatcgtttt ccgcaggttc caggccaggg aaacggcgcc
     4741 gccggtaaga aagatctccc caaacgactg gtatgtacca aagagaaaat gctcagaaat
     4801 ctctgtattg actaagatgg aaagcccgag ggttacccag attaaggctg gcaggaaatg
     4861 gcggaccggc ctgctggccg gatatttttt ccccagccag gccaggaaga aataaccccc
     4921 aaacgacacc gccagccaga atcctaacag ttgggtgata aaatcaagaa aggcgctttt
     4981 ctgttcttcc ccgggtgtca atccgaaaag cggtgcaaat ttccaccagt tgacagctac
     5041 aatggaagtc caatgccagg cctcggcggt gaagtaggac gagggcagcg gtttgaaaaa
     5101 atatgtcttc cacaaatcga tcagtcgttt ttccaccgtc gcgcggcgtt gctccagccg
     5161 caggagaaaa tcttccgcta tattggggaa aaaagcgaga agctgcctgg ccttgacggt
     5221 gctggacaac aggccgtcca gttctccgac gtaataccgg ataatgggaa cggaggcagc
     5281 caaggataca tcttccgcca atttgacgta ctcttctttt cgtccctgcg tctccttctc
     5341 aaactggtcg atcattttga tctgattctt gatggggtcg atgccacggc ggacgctttc
     5401 cttcaggaca tccatctttt tcaaggtata acggcattcc ataggggtgt tttcggaaat
     5461 gccgtggacc aggagcacat gccgcagttc ccggttcaaa tccctaatct tttcctggac
     5521 tttttttgct tccgcaggct gttcgccgat aaatttgtcc agatcatgtc gtaggtccct
     5581 tacctccgtc tgttcgcggg agatcatatc gttccaggcg gaaaaattat ccaagggcag
     5641 ggagagagag gggaacagca acaccgccaa aagcacgaag caaaaaacga attcggttgg
     5701 cgtataggtt tgatattctg aagttctatg gcacataggt ggcaaccacc ggcggagaca
     5761 atgcattttg ccggcaacgg actttaatag ttgcatcttt ttgccctatt tacgcatcgc
     5821 aaatctcacc ttgtcaggag tatttttaat gaacctgcga tgatggctcc cgttttgatg
     5881 aacgtcatgg ctccctgacc gtcttttctg gttcccagtg caacattcaa tcacattacc
     5941 tagctatttt caagaagttg tttgccttgt tccggcgata taacacctga gacaagatta
     6001 aaaaaacccg ctgtttccag cgggtttggt acgtcctcgg acatcctcgg actatgtctt
     6061 ggtggagatg agggggctcg aacccctgac ctcggcattg cgaacgccgc gctctcccag
     6121 ttgagctaca tccccttgtc gccacggtag atggcacgcc gttgtggccg ccacctttct
     6181 gactgcctcc atatatgatt ttccgtgtcg ctgtcaagag gacatatcat cgggtatgaa
     6241 cggtgcgctt ccctcattga caggtgtatt ttatttgaat cttgacaagc cttggccatt
     6301 tccctatact tccctgaaac tttccggagg ttcaccatgg caaaaatcga tgcctttttt
     6361 cagttgatga acgagcaggg ggcctcggac ctccatctcg tcactgggca gcagccagcg
     6421 atccgtatcc ggggggaaat cgagcggatc aaatatgacc tgctggacaa cgacggactc
     6481 aaggctatgc tctatgagat cgccccggag cataaaatca aacagttcga agagacggga
     6541 gacgtcgatt tcgcctatga aattcccgga ctggcacgct accgcgccaa tttctttatg
     6601 cagaagtggg gctgcgccgc cgtcttcaga gagatcccca gcaacatcct taccgccgaa
     6661 cagctcgggc tccccgcggt agtctccaaa ctggcctcct tgccccgggg actggttctc
     6721 gtcaccggcc cgaccggcag cggcaaatcc accaccctgg cggccatcat cgatcaggcc
     6781 aaccggactc gaaaggacca catcatcacc gtggaagatc ctatcgaatt cgtccacaag
     6841 agccagcagt gcattgtgaa ccaccgggag gtgggtgtcc acaccaagag tttttcctcc
     6901 gcccttcgcg gcgccctccg cgaggatccg gacatcattc tcgtcggcga aatgagagat
     6961 ctcgagacga tccgcctggc cgtggaagcg gcctccacgg gtcacctcgt cttcggcacc
     7021 ctccatacca cgtcggcagc aaaaacggtt gatcgggtca tcgaggtctt ccccgccagc
     7081 gagcaggccc aaattcgctc caccctcgcc gacggcctcc gcgccgtggt ggcccaggtc
     7141 ctcttcaaac gcatcgataa gaagggccgt tgcgccgccc tcgaaatcct catcgccacc
     7201 tccgccgtcc ggaacctgat ccgggaggcc aagaccttcc agataccttc catgatccag
     7261 acgggaaaga aatacggcat gcagcttctc gacgacgcca tctacgagtt ggtcaacaag
     7321 ggttggatcg atcccgacga ggcttacgcc aaagccaacg acaaaacaag gttccgtccc
     7381 ctgttgaaaa agcccccggc ggacttcacc gacgcataaa gacgggcggt gccggaagcg
     7441 gttaccggca gctaaagcgg ggaggctacg ggagggattt catacccgac gccgacgtcc
     7501 tcccgataat acaagctgag gcaactatgg aactttatcg ccatccacca aggatagcgg
     7561 acagggagga aagcttgaga tgagaaaaca ggaaatcgac ttcattttag ggaaaatgct
     7621 cgactcccac ggcaatgtct cggatctgaa catcaccgtg ggcaagcctt ttcaggtcga
     7681 ggcttccgga cagttgaccg gggtggaact cgatccgcct ttcagtaaac tgacgccctt
     7741 ccaatcggaa atattcgcca tgaacctgat caatcaggac caccgcctca cggaaaccct
     7801 cattgccgaa ggctcctgcg actcctccta cgaactgccc ggcaaggccc gttttcgcgt
     7861 caacatcttc tcccagcggg gcaattactc catcgtcctt cgcaagctgg aaacccggat
     7921 tcccacctgc gaggatctta atctccccaa agccttctat aagatggcgg aggaaaagaa
     7981 tgggatcatc ctggtcaccg gggccacggg aagcgggaaa tcaacctccc tggccgctgt
     8041 cttggacaag atcaacgaca ccaagtccgt tcacatcgtc accctggagg accccgtgga
     8101 attttcccat ccccacaaga aggcgacctt taaccagcgg gaaatgggaa atgatttcga
     8161 caccttcgcc agtggtctcc gggcggcgct ccgccaagcc ccgaaggtca ttctcgtcgg
     8221 ggaaatgcgg gaccgggaga cggtggaaat cggcctgtcc gccgccgaaa cgggccattt
     8281 ggtcgtcagc acccttcata ccgtggacgc cggtcagacc atcaaccgca tcctcggcat
     8341 gttttccacg gaggaggaaa cccagatccg catccgcctg gccgatacgg tccgctggat
     8401 cgtctgccaa cgcctccttc ccaaggtcgg gggcggccgg gtggcggcct tcgagatcat
     8461 gggctccaat ctccgaatca aggataccat ccttcacggc gaatcggagg gaaaaacctt
     8521 ctatgaaata atacaggcca gcaaggcctt cgggatgatc accttcgacg actacatagt
     8581 ggagctttac gagaaaggcc tgatcaccga agaaaccgcc cgggcctatg cctccaacaa
     8641 gggtatcgtc ggccggggca tcgattccgt gaaaagcacc aaaggtgagg cgaccacgga
     8701 actcggcaag ctttccatcg acaaggacta cggaaagccg aaacctaaat ggccgtaatc
     8761 gcaacgtagc gaatatccgc cggcgaacaa ccgggcaaga tcctggcagg cgaaaggact
     8821 tatctatact cgcgattctt tctaaggggg acggacaaaa ccaatggaag agcagaagac
     8881 tttggcgaaa gatgtggccg cggcggatta taatgcctcg gacatgccct tcgacttcgt
     8941 ggaggagggg gagcaaaccg ccctgatctg tgaagcggac gggtcagtgc gggaaaagat
     9001 cgttgccgcc atgaaggcga cgggataccg gaccagcgag gcggccaacg cccgggacgc
     9061 cttgaaaaag acccgttttc acgtttacga cgtcgtggtc g
//
LOCUS       DAOSMF010000074         9056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_8633_length_9056_cov_26.052328, whole genome shotgun sequence.
ACCESSION   DAOSMF010000074 DAOSMF010000000
VERSION     DAOSMF010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 9056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9056
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8633_length_9056_cov_26.052328"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1121)
                     /gene="gatB"
                     /locus_tag="PLT63_07750"
     CDS             complement(<1..1121)
                     /gene="gatB"
                     /locus_tag="PLT63_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOD98655.1"
                     /translation="MEFEAVIGLEIHIELNCPTKMFCDCPNDPAAEPNANTCPICLWF
                     PGAIPRFSQAALEKAALLCLALGAELQPRSAFDQKVYYYPDLPKGYQLSQAHLPLSRG
                     GGIDITDEDGTQKRLRIHHIHMEEDVAKLVHEMEGRTPISLVDFNRAGAPLVEIVTEP
                     DFRTPHHAMEFLKALRTQVRYAGASECSMENGTMRVDANISVRPRGTEQMNTKVEVKN
                     MNSVRHVGDAIAYEIGRQSASVQAGEEVVLHTRLWDPDKKATFPMRAKFEGPCIPDPA
                     VPVIELSPAWIETMRSRLPEMPTVRAERFMARYGLTGEEATYLSADPELASYFEALIE
                     AGIAPRTAMHWLTTQLAPAVKDRGRELGNSPVTPARLAAL"
     gene            complement(1124..>2187)
                     /locus_tag="PLT63_07755"
     CDS             complement(1124..>2187)
                     /locus_tag="PLT63_07755"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HOD98656.1"
                     /translation="DYTAREDATAVSRLRQAGAALVGLTRSSEFGFGLDGSQAGMAVR
                     SGAADLELVIDLTGESRLAAARAGICGFKTSWGIVSRYGLIGLIPSMECCGVLAGGVP
                     PIREFLRAAAGPDERDFSLPDEETLDFSGGRIDAGETTVGVVTEALDSLTPEARGAFA
                     DSLSALTAAGFTLREVSIPDFPLFSLVHQIVGAVEASSSAGRYDSVRYGRRAAGAKNW
                     NEMYLLSRGAAFGTLLKSYLFQGAFFQFQAYGAYDNACRLRARLLAAMATATAQADFL
                     VLPGENPSAPATAVTPEDIYAQFSFTAFANVTGQPALYLPPPTGEARPGWQLTGPRRS
                     DARLLAVGESLQGIRQGGK"
     assembly_gap    2188..2287
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2288..4026)
                     /gene="aspS"
                     /locus_tag="PLT63_07760"
     CDS             complement(<2288..4026)
                     /gene="aspS"
                     /locus_tag="PLT63_07760"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOD98657.1"
                     /translation="MDFSERIFCGELTREHLDREVLLCGWVDAYRDHGGLLFIHLRDR
                     SGIVQIVFSPEFTPAEVCRGAASLRTEYCLAVQGEVRKRQAGTENPNIETGHLEVFVS
                     TLTVLSEAEPLPFAISEKAMTAGASVGGAEQVNEELRMQYRYLDIRRPAMQRNLTLRH
                     RIFQCVREYLDSRGFVEVETPVLTMSTPEGARDYLVPSRLHDRSFYALPQSPQLFKQL
                     LMIGGMERYFQLARCFRDEDLRPNRQPEFTQLDIEASFVDEEYIYELLEELTVRMFAV
                     GGIALPRPFPRMTYTEAMDTTGSDRPDLRFGLCMVDATDIFAATTYGIFRQIRERGGI
                     IKGINIRGQSEKLSKNVLQNEYAKEIAPSFGAKGMTWMRVEGGKLESNIVQFFSAAEQ
                     EALKGRFQLADGDVLIMVADPSPAVVNSALGQLRLHLADRLGLIPPDVFCPVWVTDFP
                     LFEPTDDGGVTSSHHPFTAPDRTDFDPADVAELLSLHSRAYDLVVNGEELGGGSIRIN
                     DRRVQRRIFAALGLSEQEVREKFGFFLRAFDFGAPPHGGLALGMDRVASMILKTPSIR
                     EVIAFPKNRSAACP"
     gene            complement(4138..5052)
                     /locus_tag="PLT63_07765"
     CDS             complement(4138..5052)
                     /locus_tag="PLT63_07765"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOD98658.1"
                     /translation="MASRPRRLIPFLAFFFRRKVLRQKQPLLASFKLTYRCNLACRAC
                     PFHLRAGEAGDHMSWATATAGLDALRRQGVLIVVFEGGEPLLWRDGSHGIGDLIAYAR
                     RRFPRVAVTTNGTQPLDVPADLLWVSIDGLPETHDRLRSRSFARVWENLCSSRHDRLF
                     VHFTINRENRRDLRPLLDQLRKIRMFKGMTVQLFYPYGQGEAPLALDGRERREVLEAV
                     IGMKREGRPILNSAGRLRTMIANDWRCHDDLLVNVDPDGAITRGCYVKSRGRIDCRAC
                     GFSPVAEASGALDLRPGSLWAGWSIFLS"
     gene            complement(5065..6291)
                     /gene="rpsA"
                     /locus_tag="PLT63_07770"
     CDS             complement(5065..6291)
                     /gene="rpsA"
                     /locus_tag="PLT63_07770"
                     /inference="COORDINATES: protein motif:HMM:NF005208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HOD98659.1"
                     /translation="MATSENMEGNEEKSFAELFEQTPVRQDFLKPGQKVEVKIVKITN
                     DWIFLDLGGKSEGYLDRKELADEEGTVAVKEGDVLTAYFLSSRHNEKLFTTRIGAGDS
                     AREYLEEIWQSGVPVEGVVEKEIKGGYDIKLPGGMRGFCPFSQMGLARMDKGQDFLGR
                     TLAFQIVEYSERGRNIILSNRAIREAERKKEEEALKETLREGMVVSGTVVSLRDFGAF
                     VDIGGVQGLLPVSEIGWERVTDINERLTVGQVLEVAITKLDWENGRISLSLKSTLADP
                     WDEVRSTYPEGTIQRGTVVRLTPFGAFVNLGPGVDGLLHVSKLGKGKRIAHPRDAVRE
                     GDSLEVRIDAIDGERKRISLSLATEEAADQDRPGEAAAGKDEDDYRQYMRQGPKTMGS
                     LGDLMKQKASSGKKRR"
     gene            complement(6471..>7232)
                     /locus_tag="PLT63_07775"
     CDS             complement(6471..>7232)
                     /locus_tag="PLT63_07775"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOD98660.1"
                     /translation="LMGCTQEQMIFHPEVLPRDYRYNFSDSFTEVNIPVAGAVINALH
                     FRIPEARGVVLYLHGNAGSLRTWGDVARDFTSRGYELFIFDYRGFGKSTGRIDSESQL
                     LDDALAVYERLRETTPAERIVVYGRSIGTGMAAWLARTGKPGLLILESPFYSLADLAA
                     HHYPLLPRRLISLILKYPFPTDRWLPEVACPVYLIHGTKDDIIPYDAAVRLEKLIRSP
                     HELIRIEGGGHNDLSEFGAYDQVLDRLLGGTAKRP"
     assembly_gap    7233..7332
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7333..7587
                     /locus_tag="PLT63_07780"
     CDS             <7333..7587
                     /locus_tag="PLT63_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98661.1"
                     /translation="IVLLTVSLALAAGPKTYQVTGPVLEMTNDVITVQKGKEKWQIAR
                     DKDTKVKGDLKVGSKVTIEYIMKAVAVEAKADAKKDAKKK"
     gene            7609..>9056
                     /locus_tag="PLT63_07785"
     CDS             7609..>9056
                     /locus_tag="PLT63_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HOD98662.1"
                     /translation="MKDAAPALPIARGEKGDIAILPRMANRHGLIAGATGTGKTVTLR
                     VLAEQFSAVGVPVFLADVKGDLGGLCRPGGDNAKVNERIGELKLEGFEFAGYPVVFWD
                     VFGAKGHPLRTTISEMGPLLLGRILRLNDTQSGVLNLIFKIADDNGLLLLDLKDLRSV
                     LQFVGDNAEKFRTEYGNVSKASIGAIQRNLLVLEEQGGDLFFQEPALDVNDLLQTDAD
                     GRGVINIIAADRLMQSPRLYAAFLLWLLSEFFEQLPEVGDPPKPRLVFFFDEAHLLFA
                     DAPKALVEKIEQVVRLIRSKGVGVYFVTQNPLDLPETVLGQLGNRVQHALRAFTPRDQ
                     KAVRAAAETFRVNPRLDTAQAILELAVGEALVSVQDAKGIPTVVERAWILPPRSRLGV
                     LEPAEMERTIRGSVLYGHYERTLDRESAYEKLQQKAAEIQAAAAQAAPAAPAAGREKP
                     SELQKYVGAFVKSAAHSVGSQIGRQIIRGVLGS"
BASE COUNT         1525 a         2779 c         2992 g         1560 t
ORIGIN      
        1 agcgcggcga ggcgggcggg ggtgacgggg gagttgccca gctcccggcc gcggtctttc
       61 acggcggggg ccagttgcgt ggtcagccag tgcatggcgg tccggggggc gattcccgcc
      121 tcgatgaggg cctcgaaata ggaggccagt tccggatcgg cgctcaagta ggtcgcctcc
      181 tccccggtga gcccgtaccg ggccatgaag cgttcggccc gtaccgtagg catctcggga
      241 agacgggacc gcatcgtttc gatccaggcc ggcgacagct cgatgaccgg gaccgccgga
      301 tcggggatgc agggtccctc gaacttggcc cgcatgggga aggtggcctt cttgtcgggg
      361 tcccacaggc gggtatggag gaccacctcc tccccggcct ggacggaggc gctctgccgg
      421 ccgatctcgt aggcgatggc gtcgcccacg tggcggacgg agttcatgtt tttcacctcc
      481 accttggtgt tcatctgctc cgtgccccgg ggccggacgg agatgttggc atcgacgcgc
      541 atggtgccgt tctccatgct gcactccgag gctccggcgt agcgcacctg ggtccggagg
      601 gccttgagaa actccatggc gtgatggggc gtccggaagt ccggttcggt gacgatctcc
      661 acgagcggcg ccccggcgcg gttgaaatcc accaggctga tgggcgtccg cccctccatt
      721 tcatgcacca gcttggccac gtcctcttcc atatggatgt ggtggatccg cagccgcttc
      781 tgggtgccgt cttcatcggt gatgtcgatc ccgccgcccc gggacagggg gagatgggcc
      841 tgggacaact gatagccttt gggaagatcg ggatagtaat ataccttctg atcgaaggcg
      901 ctccggggct ggagctccgc gcccaaggcg agacagagga gcgccgcctt ttccaaggcg
      961 gcctggctga accgggggat ggccccggga aaccacaggc agatcgggca ggtgttggcg
     1021 ttgggttccg cggcggggtc gttggggcaa tcgcagaaca tcttcgtcgg gcaattcagt
     1081 tcgatgtgga tctccaatcc gatgacggct tcgaattcca tgtctattta cctccctggc
     1141 ggatgccttg cagtgattcc cccacagcga ggaggcgggc gtcgctccgt ctgggcccgg
     1201 taagctgcca tcccggccgg gcctcccccg tcggcggcgg taggtacagg gcgggttgcc
     1261 ccgtgacatt ggcaaaggcc gtaaaggaaa actgcgcgta tatgtcttcc ggggtgacgg
     1321 cggtcgcggg ggcggaggga ttttccccgg gcaggacgag aaaatcggcc tgggcggtcg
     1381 ccgtagccat ggcggccagc agcctggccc ggaggcggca ggcgttgtcg taggccccgt
     1441 aggcctggaa ctggaaaaag gccccctgga agaggtagct cttcagaagg gttccgaagg
     1501 cggcgccccg ggagaggagg tacatctcgt tccagttctt cgccccggcg gcccggcggc
     1561 cgtaacgcac cgaatcgtag cgcccggcgc tggacgaggc ctccacggcg ccgacgatct
     1621 gatggaccag gctgaacagg ggaaaatcgg ggatggatac ctctcggagg gtgaatcccg
     1681 ccgccgtcag ggcggagagg ctgtccgcga aggccccccg cgcctcgggg gtcagggagt
     1741 ccagcgcctc ggtgacgacg ccgacggtcg tttccccggc gtcgatccgc ccccccgaaa
     1801 aatcaagcgt ctcctcgtcg ggaagggaaa agtcgcgctc gtcgggaccc gcggcggccc
     1861 gcaggaattc ccggatcggc ggcacgcccc cggcgaggac gccgcagcat tccatggagg
     1921 ggatcaggcc gatgagaccg taacgggaga cgatccccca actggtcttg aaaccgcaga
     1981 tcccggcccg ggccgccgcc aggcgggatt cgccggtaag gtcgataacc agttccaggt
     2041 cggcggcccc gctccggacg gccattccgg cctgactgcc gtcaaggccg aagccgaatt
     2101 cactgctccg ggtcaggccg accagggccg ccccggcttg ccggaggcgg ctcaccgccg
     2161 tggcgtcttc ccgggcggtg taatcggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnggg caggcggcgg aacggttctt cgggaaggcg atgacctccc ggatggacgg
     2341 ggtcttcagg atcatggaag ccacccggtc catccccagg gccaggccgc cgtggggcgg
     2401 ggcgccgaag tcgaaggccc ggaggaagaa gccgaatttt tcccgcacct cctgctccga
     2461 aagacccagg gcggcgaaga tccggcgctg cacccgccgg tcgttgatgc ggatgctgcc
     2521 gccccccaac tcctcgccgt tgaccaccag gtcgtaggcc cgggagtgga gggagaggag
     2581 ttccgccaca tcggcgggat cgaagtcggt gcggtccggg gcggtgaagg gatggtggct
     2641 ggaggtgacg cccccgtcgt cggtaggctc gaagaggggg aaatcagtga cccagacggg
     2701 acaaaagacg tccggcggga tcaggccgag gcggtccgcc agatggaggc gcaactgacc
     2761 cagggcggaa ttcaccacgg ccggggaagg atcggcgacc atgatcagga cgtcaccgtc
     2821 cgcaagctgg aagcggcctt tcagggcctc ctgctcggcg gcgctgaaga actggacgat
     2881 gttggactcc agctttcccc cttccacgcg catccaggtc atgcccttgg cgccgaagga
     2941 cggggcgatt tccttggcgt attcgttttg caggacgttc ttgctgagct tctccgactg
     3001 gccccggatg ttgatcccct tgatgatccc gccgcgctcc cggatctgcc ggaaaatccc
     3061 gtaggtcgtc gcggcgaaga tgtcggtggc gtccaccatg caaagaccga agcgcaggtc
     3121 gggacggtcg gagccggtgg tgtccatggc ctccgtgtag gtcatccggg ggaacgggcg
     3181 ggggagggcg atgccgccca cggcgaacat cctcaccgtc aattcttcca acaattcata
     3241 gatgtattct tcatcgacga aggaggcctc gatgtcgagc tgggtgaatt ccggctggcg
     3301 gttcggccgg agatcctcgt cgcgaaaaca gcgggcaagc tgaaagtatc gctccatccc
     3361 gccgatcatg agcaattgct tgaagagttg gggcgactgg gggagggcgt agaagctccg
     3421 gtcgtggagg cggctgggga cgaggtagtc ccgggcaccc tcgggggtgc tcatggtgag
     3481 aacgggggtt tccacctcca cgaacccccg gctgtcgagg tattcccgga cgcattgaaa
     3541 gatccggtgc cggagggtca ggttgcgttg catcgccggg cggcggatgt ccaggtagcg
     3601 gtactgcatc cgcagctcct cgtttacctg ctcggcgccg ccgacggagg cgccggccgt
     3661 catggccttc tcggagatgg cgaagggcag gggttccgcc tcgctaagga cggtgagcgt
     3721 ggagacgaag acctccagat gtccagtttc gatgttgggg ttttccgttc ccgcctggcg
     3781 cttccggact tcgccctgga cggcgaggca atattcggtg cgcagggacg cggcgccgcg
     3841 gcagacctcg gcgggagtga attcggggct gaagacgatc tggacgatac cgctccggtc
     3901 ccgaaggtga atgaacagca gcccgccgtg atcccggtag gcatccaccc agccgcacag
     3961 gagcacctcg cggtccagat gctcccgggt caactccccg caaaagatac gttccgaaaa
     4021 gtccatgcgc cgatccttcc tccccgccgc ggcaccgggc cggcggccgt ttgcacaatt
     4081 ttgtaccgcg gaacgtatca aaaccccggc gatatttcaa ggtgagaatg gcgggggtca
     4141 gctcaggaag atggaccagc ccgcccaaag ggagccgggg cgaagatcca gcgcccccga
     4201 ggcctcggcg acgggggaga agccgcaggc ccggcagtcg atccggcccc ggctcttgac
     4261 gtagcagccc cgggtgatgg cgccgtcagg atccacgttg acgaggaggt cgtcgtggca
     4321 gcgccagtcg ttggcgatca tggtccggag gcgcccggcg gagttgagga tgggccgccc
     4381 ctcccttttc attccgatca ccgcctccag gacctccctc ctctcccgcc cgtccagggc
     4441 cagcggtgct tccccctggc cgtaggggta gaagagctgg acggtcatgc ctttgaacat
     4501 cctgattttc cgcagttggt ccagaagggg gcggaggtcg cggcggtttt cccggttgat
     4561 cgtgaagtgg acgaagaggc ggtcgtggcg ggaggaacag aggttttccc agacccgggc
     4621 gaaggagcgg gagcggagcc ggtcgtgggt ctccggcaat ccgtcgatgc tcacccagag
     4681 gagatccgcc ggcacgtcca gaggttgggt gccgttggtc gtcacggcca cccgggggaa
     4741 gcgccggcgg gcgtaggcga tcaaatcccc gatgccgtgg gagccgtccc gccacaggag
     4801 cggctccccg ccctcaaaga cgacgatgag gacgccctgg cgccgcaggg cgtcgaggcc
     4861 ggcggtggcc gtcgcccagc tcatgtggtc ccccgcctcc ccggcgcgga gatggaaggg
     4921 acaggcccgg caggcgagat tgcaccggta ggtcaacttg aaactggcca ggaggggctg
     4981 tttttgcctg aggaccttgc gccggaagaa aaaggcgaga aaggggataa ggcggcgggg
     5041 ccgggaagcc atcgcggcgg cgcctcagcg gcgttttttc ccgctcgagg ccttctgttt
     5101 catgaggtcg cccaaagaac ccatggtctt cggcccctgc cgcatgtact gccgataatc
     5161 gtcttcatcc ttaccggccg ccgcttcccc cggccggtcc tgatcggccg cctcctccgt
     5221 cgccagggac agggagatcc gcttgcgttc cccgtcgatg gcatcgatcc ggacctccag
     5281 ggagtctcct tcccggaccg catcccgggg atgggcgatc cgttttccct tgcccagttt
     5341 ggagacgtgg aggaggccgt ccacccccgg tccgaggttg acgaaggccc cgaagggggt
     5401 caggcgcacc accgttcccc tttggatggt cccttcggga taggtgctcc ggacttcgtc
     5461 ccagggatcg gccagggtgg acttcaggga caggctgatc cgcccgtttt cccagtccag
     5521 cttcgtgatg gccacctcca ggacctggcc taccgtcagg cgctcgttga tgtccgtgac
     5581 ccgctcccag ccgatttccg acaccgggag gagcccctgg acgccgccga tatcgacgaa
     5641 ggcaccgaag tcacggaggg aaaccacggt gccggagacg accatcccct cacggagggt
     5701 ttctttcagg gcctcttcct ccttctttct ctccgcctcg cggatggccc ggttggagag
     5761 aatgatgttg cggccccgct ccgaatactc gacgatctga aacgccaagg tccggcccag
     5821 gaagtcctgt cccttatcca tccgggccag ccccatctgg gagaagggac agaagccccg
     5881 catccccccc ggcagcttga tatcgtagcc gcccttgatc tccttttcca ccaccccttc
     5941 caccgggacc ccgctctgcc agatctcttc cagatattcc cgggcggaat cccccgcccc
     6001 gatccgggtg gtgaagagtt tttcgttgtg tctcgaagac aggaaataag ccgtcaggac
     6061 atcgccttcc tttacggcga cggtcccctc ttcgtccgcc agctccttcc ggtccagata
     6121 gccctcgctc ttgccgccca gatcgaggaa gatccaatca ttcgttattt ttacgatctt
     6181 tacttccacc ttctgcccgg gtttcaggaa atcctgccgc accggagtct gttcaaaaag
     6241 ttcggcaaaa ctcttctctt cgttgccttc catgttttcg cttgtcgcca tttcccttcc
     6301 cacctgtccg tccatatttt gttttcccgg gaatgaagcc gccttccctt ctcctctcca
     6361 tagcgacaaa tcgtctcgat gacaatgtct tttttaccac ggtcatgcgg ctaggctcat
     6421 accgtggccg gaggggtttt tgcgggtgcg ccgattctat gtccgggcgg ttatggccgc
     6481 tttgccgtcc cgcccagaag ccggtcgagg acctggtcgt aggcgccgaa ctcactgagg
     6541 tcgttgtgcc cgcctccttc gatgcgaatc agctcgtggg gtgagcggat cagcttttcc
     6601 agcctcaccg cggcgtcgta ggggatgata tcgtccttgg tgccgtggat gagatagacc
     6661 gggcaggcca cctccgggag ccatcggtcg gtaggaaagg gatatttcag gatcagggag
     6721 ataaggcgcc ggggcagcag gggatagtga tgggcggcca ggtcggcgag gctgtaaaag
     6781 ggcgactcca ggatcaggag gcccggtttg ccggtccggg caagccaggc ggccatgccg
     6841 gtgccgatgg agcggccgta aaccacgatc cgctccgccg gggtggtttc ccgaaggcgt
     6901 tcgtaaaccg ccagggcgtc gtcgaggagc tggctctcgc tgtcgatgcg ccccgtgctc
     6961 ttgccgaagc cgcgatagtc gaagatgaac agttcgtagc cccgggaggt gaaatcccgg
     7021 gccacgtcgc cccaggtacg gaggctgccg gcgttcccgt ggaggtaaag gaccaccccg
     7081 cgggcctccg ggatgcggaa gtggagggcg ttgatcaccg cccccgccac gggtatgttt
     7141 acttccgtaa atgaatccga gaagttgtag cgataatccc gggggaggac ttcgggatgg
     7201 aagatcatct gctcctgggt gcagcccatc agnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7321 nnnnnnnnnn nnatcgttct gttgaccgta tccctggccc tggcggcggg tcccaagacc
     7381 tatcaggtca ccggccccgt cctggagatg accaatgacg tcatcaccgt ccagaagggc
     7441 aaggaaaagt ggcagatcgc ccgggacaaa gacacgaagg tgaagggaga cctgaaggtc
     7501 ggttccaaag tgaccatcga gtacatcatg aaggcggtgg ccgtcgaggc taaggcggat
     7561 gcgaagaagg atgccaagaa gaaataagcg caggaaggga gagggatcat gaaggacgcg
     7621 gcgcccgcat tgccgatcgc tagaggagaa aagggggata ttgccatcct accgcgcatg
     7681 gccaaccgtc acggactcat tgccggcgcc accggcacgg gcaagacggt gaccctgcgg
     7741 gtgctggcgg aacagttcag cgccgtcggc gttcccgtct ttctggcgga cgtcaagggg
     7801 gatctagggg gcctctgccg ccccggggga gacaacgcca aggtaaacga gcggatcggc
     7861 gagctgaagc tcgagggctt tgagttcgcc ggttatcccg tggtcttctg ggatgtcttc
     7921 ggcgccaagg ggcatcccct ccggaccacg atctccgaga tggggccctt gctgctgggg
     7981 cgcatcctgc ggctcaacga tacccagagc ggagttctga acctcatctt caagatcgcc
     8041 gacgacaacg gcctgctgct gctggatctc aaggacctcc ggtccgtcct gcagtttgtc
     8101 ggcgacaacg cggagaaatt ccggacggaa tatggcaacg tctccaaggc cagcatcggc
     8161 gccattcagc gaaacctcct cgtcctggaa gagcagggcg gggacctgtt tttccaggag
     8221 ccggccctgg atgtcaacga cctcctccag accgatgccg acggccgggg ggtgatcaac
     8281 atcatcgcag cggaccggct gatgcagtcg ccgcggcttt atgccgcctt cctgctgtgg
     8341 ctcctgtcgg agttcttcga gcagcttccc gaggtgggcg acccgcccaa accccgcctc
     8401 gtctttttct tcgacgaggc ccatctcctc ttcgccgacg cccccaaggc cctggtggag
     8461 aagatcgagc aggtcgtccg gctcatccgg tccaaggggg tgggggtgta tttcgtcacc
     8521 cagaaccccc tggatctccc ggaaacggtc ctgggccagc tcggcaaccg ggtccagcac
     8581 gccctgcggg ccttcacccc ccgggaccag aaggcggtcc gggccgccgc cgagaccttc
     8641 cgggtcaatc cccggctcga cacggcccag gccatcctgg agctggccgt gggcgaggcc
     8701 ctggtgtcgg tccaggatgc caaagggatc cccaccgtcg tggagcgggc ctggattctt
     8761 ccgccccgga gccgcctggg ggtcctggaa ccggcggaaa tggagcggac catccgggga
     8821 tcggtgctgt acggccatta cgagcggacc ctggaccggg agagtgccta cgagaagctg
     8881 cagcagaagg cggcggagat tcaggccgcg gcggcccagg cggcgcctgc cgccccggcg
     8941 gccggcaggg aaaagccttc ggagttgcag aagtacgtgg gcgccttcgt aaagagcgcc
     9001 gcccattccg tggggagcca gatcggccgg cagatcatcc ggggcgtcct cggatc
//
LOCUS       DAOSMF010000075         8927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_8802_length_8927_cov_22.796100, whole genome shotgun sequence.
ACCESSION   DAOSMF010000075 DAOSMF010000000
VERSION     DAOSMF010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 8927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8927
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8802_length_8927_cov_22.796100"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2045)
                     /locus_tag="PLT63_07790"
     CDS             complement(<1..2045)
                     /locus_tag="PLT63_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOD98663.1"
                     /translation="MLYKPTLIIPGMKNGVRVDTRALEAEIQEAVWAGHRCIEIHAYG
                     QHGIGGRIWKAGDEEILIRVLGSAGQRVGSMGFPNTTIDVFGPCSDDVGWLNAGARII
                     IRGNATNGVANAMAQGKIYIAGDIGARGMTMTKHNPRFAPPELWVLGSVGDSFAEFMA
                     GGVAVICGYDTPRQENVMGYRPCVGMVSGRIFFRGPHQGYSEEDAKLSPLSDEDWQWL
                     KDNMAAFLTATGRTELYAVLTAERASWQLLTARQPYEKAARATRTMGRFRAEIWDREL
                     GAGGLIGDLTDLPRTTVAVVPTGELRRFVPFWENERHLPPCQVSCPTGIPVQKRWSLI
                     RQGKTAAAVDLALRYTPFPATVCGYLCPNLCMQGCTRRNVQLPPLDVAALGRASLEAQ
                     PPAPAPASGKTVALIGGGPAGLSAAWQLWMQGHAPVVYEYRERLGGKIAAAIPRSRIP
                     DQVVEHELRRVADHIEQVALKRPLTKKEFLKLKGKHDAVIIAVGAQKPRLIPVPGQER
                     AVSALDFLQASKAGKAQAGRRVVIIGAGNVGCDAAAEAARLGAEDITLIDIQEPASFG
                     TERQHAEAAGAKFLWPRATKAVTEQGVELADGSLLPADTVIMAVGDTPDLAFLPEEFI
                     RNRGYVVTDDRYQTSDPQVFTIGDAVRPGLLTEAIGAGRIAARAIDDLLRGRRDAYD"
     gene            complement(2057..3691)
                     /locus_tag="PLT63_07795"
     CDS             complement(2057..3691)
                     /locus_tag="PLT63_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HOD98664.1"
                     /translation="MESMGKMTPSTLSRCDLPWRIVWDREACTLCGRCTAVCPVRAIE
                     LGVHRKRVVQAAVGLPEKPGNVFTVYHGIDQRTDAAHACIGCGMCDLVCPNGAIRPVR
                     STEVDKLRFHVNQGGAPRRRGGRRNDPRSVLDEIKFIRISMLTDPALDAGRHDFELRT
                     LLGRILPAEELVKTTSEKGWTPPVREIYPLIIGGMSFGALSPNMWEGLQMGVAYLNEE
                     RGMPVRICTGEGGCPPRLLRSRFLKYVILQIASGYFGWDEIIHALPEMKEDPCAIEIK
                     YGQGAKPGDGGLLMWYKVNKLIAAIRGVPQGISLPSPPTHQTKYSIEESVAKMIQSMA
                     MVWGFRVPVYPKISATSTTLAVLNNLTRNPYAAALAIDGEDGGTGAAYNVSMNHMGHP
                     IASNIRDAYLNLVKIGKQNEIPLFAGGGIGKNGNLTANAAALIMLGASGVQIGKHIMQ
                     AAAGCLGSESDRCNICNIGLCPKGITSQDPRLYRRLDPEQVAERVVDVYASFDQELRK
                     IMAPLGRSTSLPIGMSDALGIADAAAAARLQIRHVL"
     gene            complement(3670..>4589)
                     /locus_tag="PLT63_07800"
     CDS             complement(3670..>4589)
                     /locus_tag="PLT63_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase"
                     /protein_id="HOD98665.1"
                     /translation="DRYMLDLDFMVKYKLSIRPAKTPPPGTPKRDNYLVRVYDYPPEW
                     LSLRPEEIEHRLMLTRLKLRRMGEKDGSMIIFSFWPDVIMIKEVGDPMVLAEYLGLDN
                     EEFQARIILAQGRQNTNYSIDIYACHPFFIQGFATMTNGENTAFVPIREYLTSRRYEG
                     YLGYQSDSEVFTHILHFSQKKLGMGLDMYKHLLTPLKDEELQLHPQGERLRAVKHACR
                     PLIIDGPNCVIGCLPDRTLFMVQDSKKLRPGIIGGSSGMFAFSSEMCGLDAAIPARNK
                     SEDFQPMKYDTVMVPPHRQEVVRWNQWER"
     assembly_gap    4590..4689
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4690..6194)
                     /locus_tag="PLT63_07805"
     CDS             complement(<4690..6194)
                     /locus_tag="PLT63_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOD98666.1"
                     /translation="MTTHSYEELAALHAIAKTLAQPWELRDQLEQVLGELSSRLGMKR
                     GMISLLDRDTGEAWLEVAHGIKLDGLEITYRIGEGITGKVAQTGLPLAVPNLEKERLF
                     LDRTGARRHLDRNALSFLCVPIMYDSRVVGILSADKGTQEVTDLDREMAILSSVAELI
                     AKAVHILGLEEENRRLRRLVNSQRPPSLDIIGHSKGMQEVFGLIRHVADSGTTVLILG
                     ETGTGKELIAKAIHVNSGRSKGPLVQVNCAAIPDTLIESELFGHEKGAFTGALRQHRG
                     RFEEANNGTIFLDEVAELSAAAQAKLLRVLQEKRFQPLGSPRLVTTNARIIAATNRRL
                     EECAAAGSFRTDLYYRLSVFPIYIPPLRDRGNDIILLADHFVIKYAKEMNKPVKRIAT
                     PVIDAFLSHNWPGNVRELENCIERAVLLAEGDTIEIVHLPPSLQTRNRDEGEREGGRF
                     SVVIESQERAMIVEALKEAHGNQSQAARNLGVTKRIIQYKIQKLGIDPWKYR"
     gene            6332..8917
                     /locus_tag="PLT63_07810"
     CDS             6332..8917
                     /locus_tag="PLT63_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOD98667.1"
                     /translation="MVSFSRVSSGIQGLDEVLNYLRSGDNVVLQVDDIEDYRRFVLPF
                     VEEALRNNERVVYMRFARHAPLLDPGRVTVYKLNADTGFESFSTQVHNIIRQEGRDVF
                     YVFDSLSDLLLAWATDLMIGNFFVITCPYLFELNTVAYFAILRGRHSFKTVARIRETT
                     QVLLDIYNYQGRICVHPLKAWHRYTPTMFLPHITEGDKYLPIMNSMDASRLFSFLAHK
                     SSVNAERHLDYWDRIFLRAREVLADPEAVVEKQEMIAQLSRVLIGRERRMLALARDYF
                     TLEDLLNIKDRLIGTGFIGGKSVGMLLARNILRRDGDFDWSRHLELHDSFYIGSDVFY
                     SYIVQNGWWKILMAQKTKEGYFTNARELQARMLRGVFPEEVKEQFQLMLEYYGQSPII
                     VRSSSLLEDAFGNAFAGKYESFFCVNQGTPEQRYEALEDAVRRIFASTMNEDALTYRL
                     QRGLDQHDEQMALLVQRVSGSHRRHYFFPEVAGVGLSYNTFVWNQGMDPRAGMLRIVF
                     GLGTRAVNRVENDYPRIVALDAPLVKPHGALEDIRRFSQHDVDVLNLEDNRFHSLSLS
                     QVVGEGLDARLDLIGVRDTEAAERLRATGRRVPDLWVLTFDEFMTATPFTEIMSRMLK
                     TLEEVYSYPVDIEFTANFNAEGRLQVNLLQCRPFQTKGQNVRMAIPGKIKKQRILLRQ
                     EGNFMGGSVSQPLNRIIFIDPQGYARLSLSEKYDVARLVGRLNKLIEDRENTPTALFG
                     PGRWGTTTPAMGVPVTFSEINNIAAIAEIAYQDGSLIPDLSFGTHFFQDLVEMDIFYM
                     AIYPDKKGVLFNREWLAGMANLLGELAPRDRQLEEVVRVCDARQRGLLLLSDVVTQRL
                     ICYGN"
BASE COUNT         1467 a         2763 c         2870 g         1727 t
ORIGIN      
        1 ttgtcgtagg cgtcccggcg gccccgcaat aggtcgtcga tggcccgggc ggcgatccgt
       61 cccgccccga tggcctccgt cagcagcccc ggccgcacgg cgtcgccgat ggtgaagacc
      121 tggggatcgc tggtctggta acggtcgtcg gtgacgacgt aaccccggtt gcggatgaac
      181 tcttccggca ggaaggccag atcgggggtg tcgcccacgg ccatgatcac cgtatcggcg
      241 gggagcaggg agccgtcggc aagctccacg ccctgttccg tgacggcctt cgtggcgcgg
      301 ggccagagga acttcgcccc ggcggcctcg gcgtgctgac gctccgtccc gaaggatgcc
      361 ggttcctgga tgtcgatgag ggtaatgtcc tcggcgccca ggcgggcggc ctccgcggcg
      421 gcgtcgcagc ccacgttgcc cgcgccgatg atgaccaccc gtcggccggc ctgggccttt
      481 ccggccttgc tggcctggag gaaatccagg gcgctcacgg cccgttcctg tcccggcacg
      541 gggatcagcc gcggtttctg ggcccccacc gcgatgatca cggcgtcgtg cttccctttg
      601 agcttcagga actccttttt cgtcaggggc cttttcaggg ccacctgttc gatatgatcc
      661 gccacgcgcc gcagttcgtg ttccaccacc tggtcgggga tgcggctcct ggggatggcg
      721 gcggcgatct tgccccccag ccgctcccgg tactcgtaaa ccaccggcgc atgcccctgc
      781 atccataact gccaggcggc ggagaggccc gccggaccac cgccgatgag cgccacggtc
      841 tttcccgagg ccggggcggg cgcgggcggc tgcgcctcca gcgaggcccg gcccagggcc
      901 gccacatcca gggggggcag ttggacgttc cgtcgggtgc agccctgcat gcagagattg
      961 gggcagagat agccgcagac cgtggcgggg aagggggtgt agcgtagggc caggtccacg
     1021 gccgccgctg tttttccttg ccggatcagg ctccagcgct tctggacggg gatgcccgtg
     1081 ggacaggaga cctggcaggg ggggagatgc ctttcgtttt cccagaacgg cacgaagcgg
     1141 cgcaactcgc cggtgggaac caccgcgacg gtggtccggg gcaggtccgt caaatcgccg
     1201 atgagccccc ccgctcccag ttcccggtcc cagatttccg cacggaaccg gcccatggtc
     1261 cgggtggccc gggcggcctt ttcgtagggc tggcgggcgg tgaggagctg ccaggaggcc
     1321 cgttcggcgg tgaggacggc atagagttcc gtccgtcccg tggcggtgag gaacgccgcc
     1381 atgttgtcct tcagccattg ccagtcctcg tcgctcagcg gggacagctt ggcgtcctct
     1441 tcgctgtagc cctgatgggg gccccggaag aagatccggc cgctcaccat ccccacgcag
     1501 gggcggtagc ccatgacgtt ttcctggcgg ggcgtgtcgt agccgcagat caccgccacc
     1561 ccgccggcca tgaattcggc gaaggaatcg cccacgctgc ccaagaccca gagttccggg
     1621 ggggcgaagc gggggttgtg cttggtcatg gtcatgcccc gggccccgat gtctccggcg
     1681 atgtagatct tgccctgggc catggcattg gccacgccgt tggtggcgtt gccccggatg
     1741 atgatccggg cgccggcgtt gagccacccc acatcgtcgg agcaggggcc gaagacgtcg
     1801 atggtggtgt tgggaaagcc catggagccc acccgctgcc ccgccgaccc gaggacccgg
     1861 atcaggatct cttcgtcccc ggccttccag atgcggccgc caatgccgtg ctggccgtag
     1921 gcatggatct cgatgcagcg gtgacccgcc cagaccgcct cctggatttc cgcctccagc
     1981 gcgcgggtgt ccacgcggac gccgtttttc atgccgggga tgatgagggt gggtttataa
     2041 agcatgccgg gcgctcctat agtacatggc ggatctgaag tctggcggcg gccgcggcgt
     2101 cggcgatgcc cagggcgtcg gacatgccga tgggcaggga cgtagagcgg ccgaggggcg
     2161 ccatgatctt acgcagctcc tggtcgaagc tcgcatagac gtccaccacc cgttccgcga
     2221 cctgttccgg gtccaggcgg cggtagagcc ggggatcctg ggaggtgatc cccttggggc
     2281 aaaggccgat attgcagata ttgcaccggt cggactcgga accgaggcag cccgccgcgg
     2341 cctgcatgat gtgcttgccg atctgcactc cgctggcccc cagcatgatc agggcggcgg
     2401 cgttggcggt gagattgccg ttcttgccga tccccccccc ggcgaacagg gggatctcgt
     2461 tctgcttgcc gatcttgacg agattgaggt aggcgtcccg gatattgctg gcgatgggat
     2521 ggcccatgtg gttcatggag acgttgtagg ccgccccggt gccgccgtct tcgccgtcga
     2581 tggccagcgc ggcggcgtaa ggattccggg tcaggttgtt gagcaccgcg agcgtcgtgc
     2641 tcgttgccga gatcttggga tacacgggca cccggaaccc ccagaccatg gccatggact
     2701 ggatcatctt ggccaccgac tcctcgatgg agtacttggt ctggtgggtg ggtgggctgg
     2761 ggaggctgat gccctggggc acgccgcgga tggcggcgat gagcttgttc accttgtacc
     2821 acatgagcag gccgccgtcg ccgggcttgg ccccctggcc gtatttgatc tcgatggcgc
     2881 aggggtcctc cttcatctcc gggagggcgt ggatgatctc atcccagccg aaatagccgc
     2941 tggcgatctg gaggatgacg tacttcagga aacgggagcg caggagccgg ggcgggcagc
     3001 cgccctcgcc ggtgcagatc cggacgggca tgccccgctc ctcgttcagg taggccaccc
     3061 ccatctggag gccttcccac atgttgggag acaaggcgcc gaaggacatg ccgccgatga
     3121 tcagggggta gatctcccgc accgggggtg tccagccctt ttcactcgtg gttttgacca
     3181 gttcctccgc cgggagaatc ctgcccagga gggtccgcag ttcaaagtca tggcgtcccg
     3241 cgtccagggc cgggtcggtg agcatggaga tgcggatgaa cttgatctcg tccaggacgc
     3301 tccggggatc gttgcgccgg ccgccccggc ggcggggcgc cccgccctga ttgacgtgga
     3361 agcggagctt gtccacctcg gtgctgcgga cggggcggat ggccccgttg gggcagacca
     3421 ggtcgcacat gccgcagccg atgcaggcat gggccgcgtc ggttcgctgg tcgatgccgt
     3481 gatagacggt aaagacgttg cccggcttct ccgggaggcc gacagccgcc tggacgaccc
     3541 gtttccggtg gacccccagt tcgatggccc ggacggggca gacggcggtg cagcgcccgc
     3601 agagggtgca ggcctcccga tcccagacga tccgccaggg gaggtcgcag cggctcaggg
     3661 tggagggggt catctttccc attgattcca tcttaccacc tcctgccggt gcgggggcac
     3721 catgacggtg tcgtatttca tgggttgaaa gtcctcgctc ttgttccggg ccgggatggc
     3781 ggcgtcgagg ccgcacatct ccgaggagaa ggcgaacatg ccggaagagc cgccgatgat
     3841 gccgggccgc agcttcttgc tgtcctggac catgaagagg gtccggtcgg gcaggcagcc
     3901 gatgacgcag ttggggccgt cgatgatcag gggccggcag gcgtgtttga cggcccgcag
     3961 ccgttccccc tggggatgga gctggagttc ctcgtccttg aggggtgtca ggagatgctt
     4021 gtacatatcc aggcccatcc cgagcttttt ctgagagaaa tggaggatgt gggtaaagac
     4081 ctccgaatcg gactgatagc ccagatagcc ttcgtaccgg cgcgacgtga ggtattcccg
     4141 gatcggtacg aaggccgtgt tctccccgtt ggtcatggtg gcgaaaccct ggatgaaaaa
     4201 ggggtgacag gcgtagatgt ctatggagta gttggtgttc tgccgcccct gggccaggat
     4261 gatccgggcc tggaattcct cgttgtccag ccccaggtat tccgccagga ccatggggtc
     4321 ccccacctcc ttgatcatga tcacgtcggg ccagaaactg aagatgatca tggagccgtc
     4381 cttttcgccc atccggcgga gcttgagccg ggtgagcatg agacggtgct caatctcctc
     4441 cggacgcagg gacagccact ccggtgggta gtcgtaaacc cgaaccagat agttgtcccg
     4501 tttcggtgtt cccggaggag gcgtcttggc cggccggatg gagagcttgt attttaccat
     4561 gaaatccagg tccagcatgt accggtccan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnc ggtacttcca gggatcgatg cccaacttct ggattttata ctggatgatt
     4741 ctcttggtga cgccgaggtt gcgggcggcc tgactctggt tgccgtgggc ctccttgagg
     4801 gcctcgacga tcatggcccg ctcctggctc tcgatgacga cactgaatct ccccccctcc
     4861 cgttctccct catcccggtt cctggtctgg agagaaggag gaagatgaac gatttcaatg
     4921 gtatctcctt cagccaaaag cacggcccgc tcgatgcagt tttccagttc ccggacgttt
     4981 cccggccagt tgtgggacag aaaggcatcg atgaccggcg tggcgatcct ctttaccggt
     5041 ttattcattt ctttggcgta cttgatgacg aaatggtcgg ccaggaggat gatgtcgttg
     5101 ccccggtccc ggaggggggg gatgtagatg gggaaaacgc tcaaccgata gtagaggtcc
     5161 gtccggaagg aaccggcggc ggcgcattcc tccagccgcc ggttggtggc ggcgatgatc
     5221 cgggcgttgg tggtcacgag ccggggggaa cccaggggct gaaaccgctt ttcctggagg
     5281 acccggagga gcttggcctg ggcggcggcg gacagctccg ccacttcgtc gaggaaaatc
     5341 gtgccgttgt tggcctcctc gaatcggcct cgatgctggc gcagcgcccc ggtgaaggcc
     5401 cccttttcat ggccgaacag ctcgctttcg atcagggtgt ccggaatggc cgcgcagttg
     5461 acctggacga gggggccttt gctccggccg ctgttcacgt ggatggcctt ggcgatcagt
     5521 tccttgccgg tgccggtctc gccgaggatc aggacggtcg tgccggagtc ggcgacgtgg
     5581 cggatcaggc cgaacacctc ctgcatccct ttggaatgac cgatgatgtc cagggagggc
     5641 ggccgctgcg agttgaccag gcggcgcagc cgccggtttt cctcctccag ccccaggatg
     5701 tgcaccgcct tggcgatcag ttccgccacg gaggagagga tggccatctc ccggtccagg
     5761 tccgtcacct cctgggttcc cttgtccgcc gagaggatgc cgaccacccg ggaatcgtac
     5821 atgatgggga cgcagagaaa ggacagcgcg ttgcggtcca gatgccgccg cgccccggtc
     5881 cggtccagga agaggcgctc cttttccagg ttcggaaccg ccagcggcag gcccgtctgg
     5941 gccactttcc ccgtgatgcc ctccccgatc cggtaagtga tctccaggcc gtccagcttg
     6001 atcccgtggg ccacttccag ccaggcctcg ccggtgtccc gatccagaag cgagatcatc
     6061 ccccgtttca tccccaggcg gctgctcaac tcccccagta cctgttcgag ctgatccctc
     6121 agttcccagg gctgggccag ggtcttggcg atggcgtgga gggccgccag ttcttcgtag
     6181 gagtgagtgg tcatttcgta caaaaatgga caacgataaa aattattcat ctcttataca
     6241 atttagttaa aaatagcaat gggcggcctc gatcttttct ggtatggatt ttgaaacaaa
     6301 tatcgtacca aattctttcg ggaggtcgcc catggtctct ttttcccgtg tcagttcggg
     6361 cattcaaggt ttggacgagg tcctcaatta tctccggagc ggcgacaatg tcgttctcca
     6421 ggtggacgac atcgaagatt accgccgctt tgtcctcccc ttcgtggagg aggcgttgcg
     6481 gaacaacgag cgggtcgtct atatgcgttt tgcccgccat gcccccctgc tcgatcccgg
     6541 ccgggtgacc gtctataaac tcaacgcgga tacgggattt gaatcctttt ccacccaggt
     6601 gcacaatatc atccgccagg aaggccggga tgtgttctat gtcttcgatt ccctctccga
     6661 tctccttttg gcctgggcca cggacttgat gattggcaat ttctttgtca ttacttgtcc
     6721 ttaccttttt gaactcaata cggtggccta tttcgccatc ctccgggggc gccattcctt
     6781 caagaccgtg gcccggatcc gggagacgac acaggtcctt ctggatatct acaactatca
     6841 gggccggatc tgcgtccatc ccctcaaggc ctggcaccgt tataccccga cgatgtttct
     6901 gccccacatc accgaggggg acaaatacct ccccatcatg aacagcatgg acgcctcgcg
     6961 gctcttttcg tttctcgccc acaagagttc cgtcaacgcc gagcggcacc tggattactg
     7021 ggaccggatc ttcctgcggg cccgggaagt cctggcggac cccgaggccg tcgtggaaaa
     7081 gcaggagatg atcgcccagc tctcccgggt cctcatcggc cgggagcggc ggatgctggc
     7141 cctggcccgg gactacttca ccctggagga cctgctgaac atcaaggatc gcctcatcgg
     7201 gacggggttc atcggcggca agtcggtggg gatgctcctg gcccgcaata tcctccgccg
     7261 ggacggggat ttcgactggt cgcgccatct cgaactccac gactccttct atatcggttc
     7321 cgacgtcttc tattcctata tcgtccagaa cggctggtgg aagatcctca tggcccagaa
     7381 gaccaaggag ggctacttca ccaatgcccg ggagctacag gcccggatgc tccggggcgt
     7441 cttcccggag gaggtcaagg agcagttcca gctcatgctg gaatattacg gccagtcgcc
     7501 catcatcgtc cgctccagca gcctcctcga ggatgccttc ggaaacgcct tcgccggcaa
     7561 gtacgagagc ttcttctgcg tcaaccaggg gacgccggag cagcgatacg aagccctgga
     7621 ggatgccgtc cggcgcatct tcgccagcac catgaacgaa gacgccctca cctatcgcct
     7681 ccagcggggc ctcgaccagc acgacgaaca gatggccctc ctggtgcagc gcgtctccgg
     7741 atcccaccgc cggcattatt tctttcccga agtggcgggg gtgggtctct cctacaacac
     7801 cttcgtctgg aatcagggca tggatccccg ggccggaatg ctgcgcattg tcttcggcct
     7861 gggcacccgg gcggtcaacc gggtggagaa cgattatccc cgcatcgtcg ccctcgacgc
     7921 ccccctggtc aaaccccacg gcgccctgga ggacatccgc cgcttttccc agcatgacgt
     7981 ggatgtcctg aatctggaag acaaccgctt tcactccctt tccctctccc aggtggtcgg
     8041 cgagggcctg gatgcccggc tggacctgat cggggtccgg gatacggagg cggcggagcg
     8101 cctccgggcc accgggcgga gggtccccga tctctgggtc ctcaccttcg atgagttcat
     8161 gacggccacg ccgtttacgg agatcatgtc ccggatgctc aagaccctgg aagaggttta
     8221 ctcgtatccg gtggatattg aatttaccgc caatttcaac gccgaagggc ggctgcaggt
     8281 gaatctcctc cagtgtcggc ccttccagac caagggacag aacgtgcgga tggcaatccc
     8341 ggggaagatc aagaaacagc gcatcctcct ccgtcaggag gggaacttca tggggggcag
     8401 cgtctcccag cccctaaaca ggatcatctt tatcgacccc cagggctacg cccgtctttc
     8461 cctttccgag aagtacgacg tcgcccggct ggtcggacgg ctgaacaagc tcatcgaaga
     8521 ccgggaaaat acacccaccg ccctcttcgg gccgggacgc tgggggacca ccactccggc
     8581 catgggcgtt cccgtcacct tctcggaaat aaacaacatc gccgccatcg cggagatcgc
     8641 ctaccaggac ggcagcctga tccccgacct gtcgttcggg acccatttct tccaggatct
     8701 ggtggagatg gacatctttt acatggcgat ttatcccgac aagaaagggg tgcttttcaa
     8761 ccgggaatgg ctggcgggga tggccaacct cctgggggaa ctggcgcccc gggaccggca
     8821 attggaggaa gtggtccggg tttgcgacgc ccggcaacgg ggcctgctcc tgctgtccga
     8881 cgtggtgacg cagcggctga tctgctacgg gaattagttc ggcggcg
//
LOCUS       DAOSMF010000076         8861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_8870_length_8861_cov_15.598115, whole genome shotgun sequence.
ACCESSION   DAOSMF010000076 DAOSMF010000000
VERSION     DAOSMF010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 8861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8861
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8870_length_8861_cov_15.598115"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /gene="traN"
                     /locus_tag="PLT63_07815"
     CDS             <1..746
                     /gene="traN"
                     /locus_tag="PLT63_07815"
                     /inference="COORDINATES: protein motif:HMM:NF018666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein TraN"
                     /protein_id="HOD98668.1"
                     /translation="MAMKSAYTLYSTMTTGITGASGTAAAMAESVTTTFNLLGQNVGL
                     ATVATEKLSTGVTMTTLTSELGATGVLTETSAAGATTASIAPYSIAQPLMYQLATAAS
                     VVGVIISAYVIATTVYQMVFACTEEDMVTSISLGFALCHLVGELKTGDFLGMKLKSRN
                     VYCCFNSILARLVHEQGRPQVNRGWGSAEQPDCAGFSIGEFAALDFSQMNLAEYMQYV
                     QTKSNISPAEMEAIKERAITSAGAGSPPP"
     gene            complement(750..2138)
                     /locus_tag="PLT63_07820"
     CDS             complement(750..2138)
                     /locus_tag="PLT63_07820"
                     /inference="COORDINATES: protein motif:HMM:NF014581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HOD98669.1"
                     /translation="MDDLSELLQINDRCSVSKHHSQITRLGWGVNLRDLNKEREIFIQ
                     DSERSGHFGCFGTTRIGKSKLMENIVEQDIKKGCNVVIIDPKGDPELLGRVCQAAAEC
                     GRLREVMMLNPIFPDSSIMLDPLAYYYMEEELINHIISGIKAKEDYFIGIAQEVSHAV
                     IAGFALKQRVKNEVEGKNEPMSINFLKVKQNSSWHTLTKFKESIASLPGSEDLVASME
                     QILSSPQDFFAKVSSSLRTTLSALTSGTTGKIIGKAEYNEFVRRIESGEGVILFCNTG
                     SMLTDRTAHIIGRVLLSMIKSMVGRVFASGRKLKPPLCLHLDEGHNILYRGVQELFNK
                     GGGANVWIHFYTQSISQMEEEVGLEVTTSIIDNINTWMYMLVNHPATAQYMEDSSPTK
                     KKFQSILSLGGGISLRETEEKQILASKVLQLPKRHFYLRTYGELYRGITHDVSPLMVE
                     VEMPRIAGSSPA"
     gene            2403..3446
                     /locus_tag="PLT63_07825"
     CDS             2403..3446
                     /locus_tag="PLT63_07825"
                     /inference="COORDINATES: protein motif:HMM:NF024554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HOD98670.1"
                     /translation="MTERRNGGLLGMINRLWQKMSRPKPRQQRRAKERISDHEVEEAR
                     RVNLAHLIESLGYKPRWFSAEKAMFSSPLRVEKTPSFTVSYYKGRWGWKDWGTGDSGD
                     AISFAQAYYGLSFIEAVRKLNGGFSFNVPSSAPAPSARSEYDEDKIKFVRKLHSDRTA
                     LMSEKERRIIRLYFEDKEVTLYPEMAVVMYHSFKDKKSFVAIPMPHARCIKGLECREI
                     GGTDRKTLGNKTLWYLGRKSSRVLVTESILDALAGEIVLSDDTLSLLSINGVGNVGKL
                     DGVLAKLRPKEVLLALDNDNPGREAQATAVQIASRYADRVIEVDHHIRAGVKDLHKLL
                     LPARQAKSVAKAE"
     gene            3456..3827
                     /locus_tag="PLT63_07830"
     CDS             3456..3827
                     /locus_tag="PLT63_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98671.1"
                     /translation="MEPILPPYEAEWGVRTEGHNASGVLKDTAMFLTSYGEGIFVGSL
                     NDGCGKRRTNLIKAKDHREHIIRYLEARRNLGYQVTEIWTLGKGWQAHPATMPVEVPQ
                     VRRVRLSTTLRWWQEQGPEWF"
     gene            4010..4252
                     /locus_tag="PLT63_07835"
     CDS             4010..4252
                     /locus_tag="PLT63_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98672.1"
                     /translation="MKITWFFDVVLAGGQKVRRVVVEDKEGRKHLLDIPLYPLRKGER
                     GVLLKRGAKLVPAAAAEAAVKRIIPWEEGGNLVITR"
     gene            4414..5361
                     /locus_tag="PLT63_07840"
     CDS             4414..5361
                     /locus_tag="PLT63_07840"
                     /inference="COORDINATES: protein motif:HMM:NF019348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOD98673.1"
                     /translation="MEESINKLFGGLKAPDTVTMNVREDTAGHPMIPRQKPYRFRQEP
                     MSDFLGWLKGASGFDPLLITGPTASGKSSLAEQVAARLNQPAYIVPCHERMEVPDLFG
                     RFVVRDGSMTWVDGPVIAGLKDPAGAWIVMDEFDSMEPGALLGLNPVLEGRKFIVPET
                     GELIDPVAHGAKIVCTGNTAGLGDQTGEYLATKRQNVATMGRFAVMEVGYPTKEEEIE
                     ILLLHRPDLPKPLGEGMIKVANLVREQYVAGEMECVFCTRSLVRWAHLALFFRRKKGV
                     NYIMHSLDRAIGFKYDPVIREALHQLAQRKFGNDAIIVN"
     gene            5378..5767
                     /locus_tag="PLT63_07845"
     CDS             5378..5767
                     /locus_tag="PLT63_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98674.1"
                     /translation="MSLPTNWPVTIPANAKAVVWMRSDSVSGGKDWIGIATDREFITH
                     WGKTGHVNQSRVKEKNGFIGSALSNLMAAAAGKSRKGYWVVARMTHDAPAPSLNDLPP
                     PPSKPSAIAKAVEAWQKEAFATEGEWF"
     gene            5789..6220
                     /locus_tag="PLT63_07850"
     CDS             5789..6220
                     /locus_tag="PLT63_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98675.1"
                     /translation="MYNKIAPPPGATWGICLKRRGEYWIAFQTAAAIEVRYGVKEPLV
                     EQTLPASPAIMKTLIKQREDKWFKMVAEWNPTELWVPRQPPSQPHSSPTTGGGKYRKH
                     GQLPPPPPNRPVPPPPPPKPMSIIDIVRAWQEEVPPEEEWF"
     gene            6398..7372
                     /locus_tag="PLT63_07855"
     CDS             6398..7372
                     /locus_tag="PLT63_07855"
                     /inference="COORDINATES: protein motif:HMM:NF022792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3150 domain-containing protein"
                     /protein_id="HOD98676.1"
                     /translation="MYQNILGQIVLLHLDIHVWSGRRKLAPEDLKLADGSELPPESLA
                     RLGSKKVIDPEKLNDLMAVRSEAHRLALTTGTRLMGGYAIPVGRMAELKEKLDALMSK
                     GRELKNELLDGLTQAVEEWKEKNPGWEALIDRADINPETVGRAISFSWQVYNIAPVEG
                     QEEGLEAEVSGMAGQLRHEIEVEARVLWKKSFQGKPEVTQRALRPIRAMAKKIQGLVF
                     LEPELEELVNGICDALSGLPKKGPVKGKDFTSVCGIVGLLGNIPTAVAEAEAVIAAAE
                     AEAEAEDTEGQEGNLLEEETADTQAVRTPPFMAASAQAPPPAPPAEWF"
     gene            7484..7636
                     /locus_tag="PLT63_07860"
     CDS             7484..7636
                     /locus_tag="PLT63_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98677.1"
                     /translation="MNEHKIRRRQALVNKRKRLKGRLLTAAAIMGVCVAVKAMQTYIV
                     TLIAAF"
     gene            7657..>8861
                     /locus_tag="PLT63_07865"
     CDS             7657..>8861
                     /locus_tag="PLT63_07865"
                     /inference="COORDINATES: protein motif:HMM:NF017973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98678.1"
                     /translation="MKKISTVIGALRHTLNVLADKRGLTLTVSGDTACTDGKRVNLPI
                     LPENDATAARLAEGYVDHESAHVKWTDFDSWKGYGQKHPAGMSWINLLEDVRIERLQG
                     EKYPGAAINLRQLLYEVVAKGGFPIPPPNEPVGAFELWLAARERRLVLGQDALADREQ
                     IVEADCRKRYGNDFCDRFVTLADGVGFCQSTQDCADLAEKIMDLIKNPPMPPPPPPPP
                     QTDPQSDPESGQDKGKDEDEGDEGKEGREGEDEGKGSNANADPSADQEGEEQTEGGDS
                     GSDPDSSPDDGEPSDPSPFSDGGNSQSDEGNDAGEEGEDQDGSGNAAGSGKDGEKDEE
                     SNSSTSSGSQSDDGTEDGDPSPTGPQSSNGAGGDSSAVPGDADPSPESSGIAKALKAL
                     ANAEEPEEG"
BASE COUNT         2057 a         2484 c         2594 g         1726 t
ORIGIN      
        1 cgatggcgat gaaatcggct tacaccctgt attccactat gaccacgggc ataacagggg
       61 catctggaac ggcggcggca atggcagagt cagttaccac gacattcaac ctcttgggac
      121 aaaatgttgg attggcaacg gtagcaacgg aaaaactatc aacaggcgtc actatgacca
      181 cattgacatc tgagttgggg gcaactgggg tactaaccga aacctcagca gcaggcgcaa
      241 caaccgcctc aatagcgcct tattccatag cacaaccatt gatgtaccaa ttagcgacag
      301 cggcctcggt cgtcggagtg ataatttccg cttatgtcat cgccaccacc gtttatcaaa
      361 tggtctttgc ctgtaccgaa gaagacatgg ttacatcaat atctctggga tttgccctgt
      421 gtcatctggt aggcgaattg aagaccggcg atttcctggg gatgaagctg aaaagccgca
      481 atgtttattg ttgcttcaac tccattcttg cccgtcttgt ccatgagcaa ggccggccac
      541 aggtcaatcg tgggtggggc tcggcggaac agcctgattg cgcggggttc tccattgggg
      601 aattcgctgc cttggatttc tcgcaaatga atttagcgga gtacatgcag tacgttcaga
      661 caaaatcgaa catatccccc gccgagatgg aggccatcaa ggagcgggcg ataacgtcgg
      721 caggcgccgg aagtcctccc ccataaggat cacgccgggg acgatcccgc aatgcgcggc
      781 atttcaactt cgaccatcaa cggacttaca tcatgggtaa tgccccggta aagttcgccg
      841 tatgtccgca ggtagaagtg gcgcttcggc aactgaagca cctttgacgc caatatctgc
      901 ttttcttcag tttcacgcag ggatatgccg cctccgaggg acaggatgga ttgaaacttc
      961 ttctttgtag gagacgaatc ctccatgtat tgcgccgtgg cgggatgatt gacgagcata
     1021 tacatccagg tgttgatgtt gtcgatgatg ctggttgtaa cctccaggcc gacttcctct
     1081 tccatttgtg atatgctctg cgtatagaag tgaatccaaa cattggcgcc accgcccttg
     1141 ttgaatagct cttgcacacc ccggtacagg atgttgtggc cttcgtccaa gtgcaaacac
     1201 aacggaggct tgagcttccg tccggaggcg aatacgcgcc cgaccatgct cttgatcatc
     1261 gacagcaaaa cccgcccgat aatgtgggcc gtgcgatcag tcagcatgga gccggtattg
     1321 cagaatagga tcacaccctc gccggattct atgcgtctta cgaattcgtt atattcggcc
     1381 ttgccaatga ttttccccgt cgtgcccgaa gtcagggctg atagcgtggt tctgagcgaa
     1441 gacgacactt tggcgaaaaa gtcctggggc gacgacaata tctgctccat gcttgccacc
     1501 aagtcctcgc tgcccggaag ggatgcgatt gattccttga atttcgtcag cgtatgccaa
     1561 gatgagtttt gctttacctt gaggaagttt atggacatcg gttcattttt tccctccact
     1621 tcgttcttga cccgctgctt gagggcgaat cctgctatga cggcgtgtga cacctcctgc
     1681 gcaatgccaa tgaagtagtc ttcttttgcc tttatcccgg agatgatgtg gttaatcagt
     1741 tcctcctcca tataataata tgccagggga tcgagcatga tagagctgtc cggaaaaatc
     1801 ggattcagca tcatgacctc cctgagcctt ccgcattcgg cagcagcctg gcatacccgg
     1861 cccagtagct cgggatcgcc ctttggatca atgatgacta cgttacagcc cttcttgatg
     1921 tcttgctcga cgatattctc catcagcttg gacttgccga tccgcgtggt gccaaaacag
     1981 ccgaaatggc cgctacgttc ggaatcctgg ataaagattt ctcgttcctt gttgagatcc
     2041 cgtagattta ccccccatcc taagcgggtt atctggctgt gatgcttgct caccgagcac
     2101 ctgtcattga tttgaagcaa ttcagataga tcgtccatac attcctccat gctcgtatat
     2161 tacttcccct gccatgattt tcgtcatttt cccctttccg ggaagtatta tataggcagt
     2221 tagttatttt cgtagggtaa ccgaaaaggg cgcaagacgc cattgatgca gaaatgccaa
     2281 tggatgtttt gcgccttttt ttcgtttcgg caagtcctga ccctctccgg gggcaggcac
     2341 ggaacgtagg ttcagcctgt aagctcctga cccgtaaggg caggcatggc aaaggaggaa
     2401 aaatgacgga aaggaggaac ggcgggttgt tgggaatgat taaccggctg tggcagaaga
     2461 tgtcacggcc caaaccccgg caacagcgcc gggcaaaaga acgcatttcc gatcatgagg
     2521 tcgaggaggc ccgaagggtc aacctcgccc acctgatcga aagcctcggc tacaagccga
     2581 ggtggttcag cgcggagaag gcgatgtttt cctcgccgct ccgggttgaa aaaacgccgt
     2641 cgttcaccgt ctcgtactac aagggacggt ggggttggaa agattgggga accggagatt
     2701 ccggtgacgc aatctctttc gcccaggctt actacggcct cagcttcatc gaggccgtca
     2761 ggaagctcaa cggcggattt tcgttcaacg tcccgtcgtc cgccccggcg ccatcggccc
     2821 ggtccgagta cgacgaggac aagatcaagt tcgtgcggaa gctgcacagt gaccgcacgg
     2881 ccctgatgtc cgagaaggag cgccgaatca tacggctcta cttcgaggac aaggaagtga
     2941 ctctttatcc cgaaatggcg gtggtcatgt accactcctt taaagataaa aagtcattcg
     3001 tcgccatccc gatgccccac gcccgctgca ttaagggttt ggaatgtcgg gagatcggcg
     3061 gcacggaccg gaagacttta ggcaacaaga ccctgtggta tttgggtcga aagtcttcac
     3121 gagtgctggt cacggagtcg atccttgacg ccctggccgg cgagatcgtc ctttccgatg
     3181 ataccctatc gctgttgtca atcaacggcg tcggaaacgt cgggaagctg gacggcgtcc
     3241 tggcgaagtt gaggcccaaa gaggtactcc tcgccctgga caacgacaat ccgggccgtg
     3301 aggcccaggc aacggctgtg cagatagcgt cgcgctacgc ggacagagtg atcgaagttg
     3361 atcaccatat ccgtgcaggc gtaaaggatc tgcacaagct gttactgccg gcgcgacagg
     3421 caaaatctgt cgcaaaggcg gaataagggg gcgtagtgga accaatactc cccccctacg
     3481 aggccgaatg gggcgtccga accgaaggcc ataatgcttc gggcgtcctg aaagacacgg
     3541 cgatgttcct gacctcttat ggggagggaa tattcgtcgg gagcctcaat gatggctgcg
     3601 gaaagcgccg cacaaacctc attaaggcaa aagaccatcg ggagcacata ataagatacc
     3661 tggaggccag gagaaatctc ggctatcagg tcaccgaaat atggactctc ggaaaaggat
     3721 ggcaagccca tcctgccaca atgccagtgg aggtcccgca agtcaggcgg gtcaggctgt
     3781 ccacgacatt gaggtggtgg caggagcaag gccccgagtg gttctaagcc ggggcgaggc
     3841 gtcctgccac ctccgtaaaa tccgataact cccctctctg caacctgggc gtgtacctgc
     3901 gggacacttt agcatcccgc atggggatgt gctcacagtt gcggaaccgg ggtctcaagc
     3961 caggcaagac ctgaacctcg caagggcagg catggcaaag gagaaaggga tgaaaatcac
     4021 ctggtttttt gatgtcgttt tggcaggcgg gcagaaggtc cgccgcgtgg ttgtagagga
     4081 caaggagggg cggaagcacc tcctggatat acccctgtat ccccttcgca agggggagag
     4141 gggggttctc ctcaaaagag gggcaaagtt agtgcccgcc gccgccgccg aggcggcggt
     4201 caaacgaatc attccgtggg aagaaggagg aaacctcgta ataacgaggt aaacttcctc
     4261 ttccccgttc ctatccccgc aaggggggtc tattgccctc cttacgggca cgtaatactt
     4321 gggggaagct cccgagggcc tggcgagccg tcaccgctat ttctcaagcc tgtaagccct
     4381 gaaccgaatg gcaggcatgg caaggaggaa aagatggaag aaagcataaa taagctgttc
     4441 gggggtctca aggcccccga tacagtaacc atgaatgtcc gggaagatac tgccggacat
     4501 ccgatgatcc ccagacagaa gccgtatcgg ttccgtcagg agccgatgtc cgactttctc
     4561 gggtggttga agggggcaag cgggtttgac cccctcctca tcaccggccc caccgcctcc
     4621 ggcaaatcca gcctggcaga gcaggtggca gcgcggctga atcagcccgc ctacatcgtg
     4681 ccctgtcacg aacgtatgga ggtgcccgac ttgttcgggc gcttcgtcgt ccgggacggg
     4741 tcgatgacct gggttgatgg tccggtcatc gcggggttga aagaccctgc cggtgcctgg
     4801 atcgtcatgg atgagttcga ctcaatggag ccgggcgctc tgttgggtct taacccggtg
     4861 ctggagggac ggaagttcat cgttcccgaa accggagaac tcatcgaccc cgttgcccac
     4921 ggggcgaaga tcgtctgtac cggcaacact gccggtctgg gcgatcagac cggagagtac
     4981 ctcgccacca aacggcagaa cgtcgccacc atgggacggt ttgccgtgat ggaggtgggt
     5041 tatcccacca aggaggagga aatcgaaatc ctcctgttac accgcccgga cctccccaag
     5101 cctcttgggg agggaatgat caaggtcgcc aatctcgtcc gcgagcaata cgtggcgggc
     5161 gagatggagt gcgtcttctg caccaggtcc ctggtgcgat gggcacacct ggcgctcttc
     5221 tttcggcgga aaaagggagt gaattacatc atgcactccc tggaccgggc tatcgggttc
     5281 aaatatgacc cggtaatcag ggaggcgttg caccagcttg cgcaacgcaa gttcgggaac
     5341 gacgccatca ttgtgaatta gggagggggg tgataccatg tcccttccca caaactggcc
     5401 ggtaaccatt ccggccaatg ctaaagcagt cgtctggatg cggagtgact ccgtgtccgg
     5461 cggcaaggac tggatcggca ttgccaccga cagagagttt attactcact ggggtaaaac
     5521 tggccatgtc aatcagtcgc gggttaagga gaaaaatggg ttcatcggct cggctctcag
     5581 caacctgatg gctgccgctg ccggcaaaag ccgaaagggg tactgggtcg tggcaagaat
     5641 gacccatgat gccccggctc cttccctcaa cgatctcccc cctcctccct ccaagccctc
     5701 cgcgatagct aaggctgtcg aggcctggca aaaggaagca ttcgccaccg aaggcgaatg
     5761 gttctaaacg gaaggaggtg gggcagacat gtacaataaa atagcccctc cccctggcgc
     5821 tacctgggga atctgcctca aaaggcgcgg cgaatattgg atagccttcc aaaccgccgc
     5881 cgccatcgag gtcagatacg gggtgaaaga accgcttgtg gagcaaacac tccccgcctc
     5941 tccagcgatc atgaaaactc tgatcaagca gagggaggac aagtggttca aaatggtggc
     6001 cgaatggaat ccgacagaac tgtgggttcc acgacagccg ccaagtcaac cccactcctc
     6061 accaacaacg ggcgggggga agtaccggaa gcacggacag cttccccctc ccccgccaaa
     6121 ccgccctgta ccgccccctc ctccgccgaa gcccatgtcg atcatcgaca tagtgcgggc
     6181 gtggcaggaa gaggtgccac cggaagagga atggttctaa acggggcttt gtccaaggcc
     6241 ccggaaaaac cctaaacatt ataataatgt caaaacctgg gcgcgtacct gcggggcgct
     6301 atagcatccc gcacggggtg tgctcacagc tttggcattt atttccccgc ctgtaagccc
     6361 tgaaccagca gtggcaggca tggcaaagga ggaaaagatg taccaaaata tactggggca
     6421 gatagtcctg ctccacctcg atattcacgt ctggtccggg cgccggaagt tggccccgga
     6481 agatctgaaa ctggcggacg gctcggaact ccctccggag tccctggccc ggctcggaag
     6541 caaaaaggtc atcgacccgg aaaagctgaa tgacctgatg gccgtcaggt cagaggctca
     6601 ccgcctcgcc ctgacgacgg gaacccgcct catgggtggg tacgccatcc cagtagggag
     6661 gatggcggag ctgaaggaaa agctcgatgc cctgatgtcg aagggccgcg agcttaaaaa
     6721 cgaactcctt gatggcctga cccaggccgt cgaggagtgg aaagaaaaga atccgggatg
     6781 ggaggccctt atcgaccggg ccgacatcaa cccggagacc gtcggacggg ccatctcctt
     6841 ctcatggcag gtgtataaca tcgcacctgt cgaggggcag gaggaaggtc ttgaggcgga
     6901 ggtcagcggc atggccggac aactccgcca cgagatcgag gtggaagccc gcgtcctctg
     6961 gaagaagtca ttccagggga agccggaggt tactcaaagg gccctccggc ccatccgggc
     7021 catggctaag aagatccaag gcttggtctt cctcgagccg gaactggagg aactcgtgaa
     7081 cggcatctgc gatgccctct ctggacttcc gaagaaggga cccgtcaaag ggaaggactt
     7141 tacctctgtg tgcggaatcg ttggcctcct cggaaacatt cctaccgcag tggcggaggc
     7201 ggaggcggtt attgctgccg ctgaagctga agccgaggcc gaggatacgg agggacagga
     7261 gggcaacctt ctggaagagg aaaccgccga tacgcaggcg gtaagaaccc ctccctttat
     7321 ggcagcatcc gcccaggctc ctccgccggc cccgcctgct gagtggtttt aatgaaacgg
     7381 gggcatgact ggcggaaacg tcaccattcc cccgacattg catcgttttc ccgcctgtaa
     7441 gctcctgaac ctcgcaagag gcaggcatgg caaaggagta aagatgaacg agcataaaat
     7501 ccggcgccgg caggcgcttg tcaacaagcg gaaacgcttg aaaggcaggc tcctgaccgc
     7561 tgccgccatc atgggtgtct gtgtcgccgt aaaagcgatg cagacctata tcgtaaccct
     7621 gatagccgct ttctgaggcg gcgaaaggag gagaaaatga aaaagatttc cacggtgatc
     7681 ggcgccctgc gccacaccct caacgtcctc gccgacaagc ggggcctcac acttaccgtt
     7741 agcggcgaca cagcctgcac ggacggcaag cgcgtcaacc tcccaattct gccggagaat
     7801 gacgcgacgg cggcccgcct cgcggagggc tacgttgatc acgagtcggc ccacgtaaaa
     7861 tggaccgact tcgactcatg gaagggatat ggtcagaaac atcctgcggg gatgtcatgg
     7921 ataaacctcc tggaggacgt tcgcatagag cgcctccaag gtgaaaaata tccgggggcg
     7981 gcgatcaacc tccgacagct tctctacgaa gtcgtcgcaa aggggggatt ccccattcct
     8041 cccccgaatg aaccagtcgg agcgttcgag ttatggctgg cggcccgtga acggcggttg
     8101 gtcctggggc aggacgccct ggcggaccgc gaacaaatcg tcgaggccga ttgccggaaa
     8161 cggtacggca acgacttttg cgaccgcttc gtaaccctgg cggacggtgt gggtttttgc
     8221 cagtccactc aggactgcgc agacctggcg gagaagatca tggatttgat caagaatccg
     8281 ccgatgccgc cgccccctcc cccgcctcca caaactgatc cccagtccga ccccgagtct
     8341 ggtcaagaca aggggaagga tgaggatgaa ggcgacgaag gcaaggaagg ccgggaaggc
     8401 gaggatgaag gcaaaggcag caatgccaat gcagatccct ctgccgacca ggaaggcgag
     8461 gagcaaacgg aaggcggcga ctcagggagc gatcccgatt cgtcgcctga cgacggcgaa
     8521 ccgtccgatc cctccccctt ctccgatggc ggcaattccc agtccgacga agggaacgac
     8581 gccggcgagg aaggcgagga ccaggacggc tccggtaacg ccgccggttc cggcaaggac
     8641 ggagagaagg atgaggaaag caactcttcc acatcctccg gctcccagtc cgacgacggg
     8701 accgaagatg gtgatccgtc cccaaccgga ccccaaagtt ccaacggggc tggtggggat
     8761 agttccgccg tacccggcga cgccgatccc tcccccgaat cttcaggcat agccaaggct
     8821 ctcaaggccc tggcgaacgc cgaggaacca gaggaagggg a
//
LOCUS       DAOSMF010000077         8855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_8876_length_8855_cov_20.374659, whole genome shotgun sequence.
ACCESSION   DAOSMF010000077 DAOSMF010000000
VERSION     DAOSMF010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 8855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8855
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8876_length_8855_cov_20.374659"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..922)
                     /locus_tag="PLT63_07870"
     CDS             complement(<1..922)
                     /locus_tag="PLT63_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOD98679.1"
                     /translation="MKTNRDVVNKKNILCIGAGYVGGPTMAMIAAKCPDYRVTVVDIN
                     TQRIAAWNSANLPIYEPGLDELVRTVRGRNLFFSTDIDGGIRDNDIIFVSVNTPTKTF
                     GAGAGMAADLQYWEKTARQILENSISNKVVIEKSTLPVKTALAMERILNSQPNGISFD
                     VLSNPEFLAEGSAIHDLENPDRILIGSRETERGRSARNSIVEIYARWIPRDRIITTNI
                     WSSELSKLVSNAFLAQRISSINAISALCEKTEADVTEVARAVGMDSRIGAKFLNASVG
                     FGGSCFKKDILNLVYLCRFYGLEEVADYWES"
     gene            complement(939..2120)
                     /locus_tag="PLT63_07875"
     CDS             complement(939..2120)
                     /locus_tag="PLT63_07875"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOD98680.1"
                     /translation="MNATPDMMFDVLIIGAGVVGLALARQLAHRDRKLVVLERNERFG
                     QETSSRNSEVIHAGIYYPREFLKSRLCVEGNGLLYAWCRRHDIPHARIGKLIVAVNED
                     EEAELARIKAMAAGNGVSDLEILGKRRLRSLEPAVAAQAALLSPTTGIVDSHRLMQSL
                     LAAAETQGAILSCRAGVTAIHRIAGGYELEINHGAYRVATKILINSAGLHADAVAYRA
                     GIDIDAAGYRLRPCKGNYLRPSPSPSLRHLVYPLPLKNNVGLGIHATVDLSGRIRFGP
                     DSRYLNGEEIRMLQDGEIAKTNPTAEKANLYAIDEGREKDFAAAAGKYLPGITEAILF
                     PDMSGIRPKLQGPGEPAADFLIREESDRGFPGLIDLIGIESPGLTACLAIAAYVEKNF
                     F"
     gene            complement(2117..3058)
                     /locus_tag="PLT63_07880"
     CDS             complement(2117..3058)
                     /locus_tag="PLT63_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOD98681.1"
                     /translation="MKRILITGGAGFLGSHLCDRLLAQGHDVLCLDNLFTGNKNNIRH
                     LIGHSNFEFVRHDITQPIYLEVDEIYNLACPASPVHYQYNPIKTIKTSVMGAINTLGL
                     AKRVKAKILQASTSEVYGDPEIHPQHEEYWGRVNPIGIRSCYDEGKRAAECLMMDYRR
                     QNGLRIKIVRIFNTYGPRMAIDDGRVVSNFIVQALRGQDITVYGDGSQTRSFCYCDDL
                     IDGIIRMMDTPDEVVGPVNLGNPSEFTIFELAENVIRLTGSTSRIVFRPLPQDDPAQR
                     RPDIARAERLLSWQPRVPLAEGLAKTVAYFREVLFSQ"
     gene            3325..4185
                     /gene="rsmI"
                     /locus_tag="PLT63_07885"
     CDS             3325..4185
                     /gene="rsmI"
                     /locus_tag="PLT63_07885"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOD98682.1"
                     /translation="MTDNLEKRSLGKLYVTATPIGNLEDITLRAIRILREVDEIYAED
                     TRRTRKLLQAYQIDTPIYSLYDAVEKKRSRTVIDRLAAGSSIAYVSDAGTPGISDPGY
                     LLISEALQAGMPVVPVPGVSAVVAALSASGLPMHAFVFQGFLPAKPGQRRRMLNDCRT
                     EKRTMVFYESPRRLRSCLEDIGKLMGTRPIVVARELTKLHEEFIRGNAEEILETLSGR
                     SLKGEITLIIGGRQEKERGYSDEELMDMLRELGREASLSLRDRVARVTEMTGLPRKNI
                     YQIALTGKFS"
     gene            4236..4478
                     /locus_tag="PLT63_07890"
     CDS             4236..4478
                     /locus_tag="PLT63_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98683.1"
                     /translation="MSERVRGPEDMIYDLVDVVEEGNPDLGDMSVRKPKIALVNPNLQ
                     EEMLKKVEETTERLVRELFPVIAERIIREEIEKLKQ"
     gene            4524..5135
                     /locus_tag="PLT63_07895"
     CDS             4524..5135
                     /locus_tag="PLT63_07895"
                     /inference="COORDINATES: protein motif:HMM:NF017459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF799 family lipoprotein"
                     /protein_id="HOD98684.1"
                     /translation="MKKIERGRNLRRCLLIAMLAISLITGCAKKIPHFLTPEYDKRGV
                     RLIAILPVANQSKNAEAAALFRDKVLEAVYFQGYPKIPLNAIDEKLSPFFNDYRRPTP
                     ETMPPRQVGELLGVDAVLYVTLNECDTSFLLLYAATEVTATFDLRSTKTNESIWNTKY
                     SLTERNFEITRDRLRMKSCQVFEPAMEEIITKAMETLPLGPDA"
     gene            5153..5908
                     /locus_tag="PLT63_07900"
     CDS             5153..5908
                     /locus_tag="PLT63_07900"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HOD98685.1"
                     /translation="MKYKLILSFFFIAMLTWLIPGDLLAGRPLTVKVDKGEAVVSHLE
                     GEVQVLPQGKDTWRILREKANLWGGDEIVVGSNSRLEITLAGDSRLRFAEETHLHLER
                     AGAGKSADVKVHLVVGRTWAKVSKTMGLKRKFEISCDNAVTGVRGTIYRLNVHRDASA
                     LVRVYEGEISVTGATKAMETETTLGPPSKITGPHKITGPHKVSREEWTMIIRSMQQVY
                     IRADGSADKPQAFSAEEDRDAWVDWNRSRDRVL"
     gene            5908..6465
                     /locus_tag="PLT63_07905"
     CDS             5908..6465
                     /locus_tag="PLT63_07905"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOD98686.1"
                     /translation="MDAGRINIPNTLTVLRIILVPAIVILLMQGDFFKALILFTLSGI
                     TDGLDGFLARVLHQQTELGAYLDPIADKALMVSCFVTLSIKHVIPGWLSVIVVSRDCI
                     ILIGIAMLAIMSVSFQIRPTMISKLTTVFQLLTIFVVLLVHTLAIVVDFRMVLLGLYI
                     VTSALTVLSGLNYIFKGIRFINSAS"
     gene            6539..6613
                     /locus_tag="PLT63_07910"
     tRNA            6539..6613
                     /locus_tag="PLT63_07910"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6571..6573,aa:Val,seq:gac)
     gene            6683..8602
                     /gene="thrS"
                     /locus_tag="PLT63_07915"
     CDS             6683..8602
                     /gene="thrS"
                     /locus_tag="PLT63_07915"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOD98687.1"
                     /translation="MMREITITWPDRQQSTLPNGVAVKDAIAVWKPELLATTVAVKIN
                     GKTVDLSAQVSEDALVELIDVASKEGLSILRHSASHIMAQAVQDIFPGAKVSIGPSIE
                     DGFYYDFEYETTFTPEDLQKIEQRMKEIAATDAPFSRRELSREEAVALFQDKGETYKV
                     ELINDLPPDVRTVSVYTQAGYEDLCRGPHLPSTIMVKAFRLLNVAGAYWRGDERNRML
                     QRIYGTCFATQEALDEYLRMMGEAKKRDHRRLGRELDLFQMNDEAGPGLIIFHPKGMM
                     LRYLIEEWERQEHLKRGYDMVMGPQILKDDLWKRSGHFDHYRENMYFTEVEGQGYGIK
                     PMNCLSHMLIYKAKIRSYRDLPLRYFELGTVHRHEKTGVLHGLMRARQFTQDDAHILC
                     TPEQLNEEIRAIADFVAYAMKIFGFEYEVELSTRPEKSIGSDADWELATSALEGALKD
                     GRMGYEINEGDGAFYGPKIDFKLKDALKRKWQCATIQCDFTLPERFDLSYVGSDGEKH
                     RPVMLHRVILGAIERFMGVLIEHYAGAFPVWLSPVQASILTVTEKQIPYGDQVHQALV
                     DAGIRVEKSFTNDKLGYKIREAQMQKMPYMLVIGDREVAERTISPRRRDGKNMGAMTV
                     TDFIAHVREEVSNYR"
     gene            8641..>8855
                     /gene="infC"
                     /locus_tag="PLT63_07920"
     CDS             8641..>8855
                     /gene="infC"
                     /locus_tag="PLT63_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012607480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOD98688.1"
                     /translation="MNINEEIKAVQVRLIEEGRQLGILPLNEALAIAAKAGLDLVEIA
                     PNADPPVCRVMDYGKYRYQQSKKLQDA"
BASE COUNT         1997 a         2318 c         2623 g         1917 t
ORIGIN      
        1 cgctttccca gtagtcggcc acctcttcca gaccgtagaa ccggcagaga tagacgagat
       61 tgaggatgtc cttcttgaag caggagccgc cgaagccaac gcttgcgttg aggaactttg
      121 cgccgatccg gctgtccatg cccacggcgc gggccacctc cgtgacatcc gcttcggttt
      181 tttcgcacag ggcggagatg gcgttgatgg aagagatccg ctgggccagg aaggcgttgg
      241 acaccagttt ggacagttca ctgctccaga tattggtggt gatgatccgg tcccggggga
      301 tccagcgggc gtagatttcc acaatggagt tccgggccga tcttccccgc tccgtttccc
      361 gggagccgat gaggatgcgg tccggatttt ccagatcatg aatcgctgat ccttcggcga
      421 gaaattctgg gttggacagg acatcgaagg aaatgccgtt gggctggcta ttgaggatcc
      481 tctccatggc cagggccgtc ttgaccggta gggtgctctt ttcgattaca accttgttcg
      541 agatggaatt ttccaggatt tgacgtgctg tcttttccca gtactggaga tccgctgcca
      601 tgccggcacc ggcgccaaag gtcttcgtgg gggtgttgac gctgacaaag atgatgtcgt
      661 tgtcgcgaat ccccccgtcg atgtcggtgc tgaaaaagag attcctcccc cgcacggtcc
      721 ggacgagttc atccagtcca ggctcgtaga tcggcagatt agcggaattc caggcggcga
      781 tccgctgggt atttatgtcc accaccgtca cccggtagtc gggacacttg gcggcaatca
      841 tggccatggt gggtccgccg acatagcccg ctccgatgca gaggatgttc tttttattta
      901 ctacatcacg atttgttttc atggttttct ctcccggatc aaaaaaaatt cttttccacg
      961 taggcggcaa tagccagaca ggccgtcaat cctggggatt cgataccgat gagatcgatg
     1021 agcccgggaa agccccggtc gctctcctcc cggatgagga aatccgctgc cggttcgccg
     1081 ggaccctgca gcttgggacg gatgccgctc atgtccggga agaggatagc ctcagtaatc
     1141 cctggaagat acttaccggc ggcggcggcg aagtcctttt cccggccttc gtcgatggcg
     1201 taaagattgg ctttctcggc ggtgggattg gttttggcta tttcgccgtc ttgcagcatg
     1261 cggatttcct caccgttcag gtaacggcta tccggtccga aacggatacg acccgacaga
     1321 tccacggtgg catgaatgcc cagcccgaca ttgttcttca ggggaagggg atagacgaga
     1381 tgccggagcg acggggaggg ggaaggacgg agataattgc ccttgcaggg ccgcagacgg
     1441 tagcccgcgg cgtcgatgtc gattccggcc cggtaggcta cggcatcggc gtggagtccc
     1501 gcactgttga tcaagatctt cgttgccact cggtacgccc cgtggtttat ttccagttcg
     1561 tagcctccgg cgatgcggtg gatagccgtg acgccagcgc ggcaggaaag gatcgcccct
     1621 tgagtctccg cggcggcaag cagggactgc ataaggcgat gactgtccac aatccccgtg
     1681 gtaggcgaga gcagggcggc ctgggccgca accgccggct ccagagaccg gagccgcctt
     1741 ttccccagaa tttccaggtc cgagacacca ttgccggcgg ccatagcctt gatgcgggca
     1801 agctccgcct cctcgtcttc attgacggcg acgatgagtt tgccgatccg ggcgtgaggg
     1861 atgtcatggc ggcggcacca ggcatagaga agcccgtttc cttccacgca caggcgactc
     1921 ttcagaaatt ctcggggata gtagatcccg gcatgaatca cctcgctgtt ccgggaactc
     1981 gtttcctgtc caaaacgctc gtttctttcc aggaccacca gttttctgtc ccggtgggcc
     2041 agttgcctag ccagggcgag gccgacaacg ccggcgccga tgatgagcac atcgaacatc
     2101 atatccgggg tggcgttcat tgtgaaaaca acacttcccg gaagtatgcg accgtcttgg
     2161 cgagaccttc ggccagcggc acccgaggtt gccacgacag gagacgttcc gcccgggcaa
     2221 tgtcggggcg gcgctgtgcg ggatcgtcct ggggcagggg ccgaaagacg attcgggaag
     2281 tcgatccggt gaggcggatc acattctccg ccagttcgaa aatggtgaat tccgacggat
     2341 tgccgagatt cacgggtccg acgacctcat cgggagtgtc catcatcctg atgataccgt
     2401 cgattaggtc gtcgcaatag cagaaggagc gggtttggga tccgtcgccg taaacggtta
     2461 tgtcttgtcc cctcagggcc tgaacgatga agttgctgac cacccgtcca tcgtctatgg
     2521 ccatgcgtgg accatatgta ttgaatatcc taactatttt tattcgcaac ccattctggc
     2581 ggcggtaatc catcatgaga cattccgcgg cccgtttacc ctcgtcgtag cagctgcgga
     2641 taccgatggg attgactcgt ccccagtatt cctcgtgctg gggatggatt tccgggtcgc
     2701 catagacctc tgaagtcgag gcctgaagga tcttggcctt taccctcttt gccagcccca
     2761 gtgtattaat cgcgcccatg acactggttt ttatggtctt gatgggattg tactgataat
     2821 ggaccggcga ggcgggacaa gcgaggttgt agatctcgtc cacctccaaa tagatcggct
     2881 gggtaatgtc gtggcggacg aactcgaagt tggaatggcc gatcagatgg cggatattgt
     2941 tcttgttgcc ggtaaagaga ttgtccagac acagaacgtc gtgaccctgg gcaaggagcc
     3001 ggtcgcagag gtgggagccc aaaaaacccg cgccgccggt gatcaatatt cgtttcatct
     3061 tcatggcgga aagaccttgt taaaatacat atgccacctg ttttctcccc tatccgcgga
     3121 ggggagaaaa cagcggccaa gccaagtcgc gtttccagat ggtaaaacgg aaaattttcg
     3181 gttatataaa gggtcgtctt ggcagtgtca agacaattaa gaacatcaat ccttgtttca
     3241 ggcctctcac gacgcgggca gggcacggaa aggagaagat aatgcagcgg ctgtaaagcc
     3301 ctttacggcc gccggcccgg aaaaatgact gacaaccttg aaaagagatc cctgggaaaa
     3361 ctttacgtta cggccacgcc cataggcaac ctcgaggata tcaccctgcg agcgatccgc
     3421 atcctccggg aagtggatga gatctacgcg gaagacaccc gccggacaag aaagctgctc
     3481 caggcttacc agattgacac cccgatttac agcctctacg atgccgtgga aaagaaacgg
     3541 tcgcggaccg ttatcgaccg actggcggcg ggaagcagca tcgcctatgt ctccgatgcg
     3601 gggactcccg ggatatccga tcccggttat ctcctgatca gcgaggccct gcaggccggg
     3661 atgccggtgg ttcccgtccc tggcgtttcg gcggtcgtcg cagcgcttag cgcctcgggg
     3721 ctgcccatgc atgccttcgt attccagggt tttttgcccg cgaagcccgg acagcgccgg
     3781 cgaatgctga acgactgccg taccgagaag cggaccatgg tcttttatga atccccgcgg
     3841 cggctccggt cgtgtctgga agacatcggg aagctcatgg gaacgcgacc catcgtcgtc
     3901 gcccgggagc tgacgaaatt acacgaggag ttcatccggg gcaacgccga ggagattctc
     3961 gagacgcttt ccgggaggag cctcaaagga gagatcaccc tgattattgg aggccggcaa
     4021 gaaaaggagc ggggatacag cgatgaagaa cttatggata tgctccggga gttgggccgg
     4081 gaggcttctc tcagcttgcg ggaccgggtg gcgagagtca cggagatgac ggggcttccc
     4141 cggaaaaata tctaccaaat tgctttgaca gggaaatttt cttgaactgt gatcgcgggt
     4201 atggtagcag agacgaaaac ctgggaggag acaggatgag cgaacgggtt aggggaccgg
     4261 aggacatgat ctatgatctg gtcgatgtcg tcgaagaggg gaatccggat cttggcgaca
     4321 tgtcggtgcg aaaaccgaag atcgccctgg tgaatcccaa tcttcaggaa gaaatgctca
     4381 agaaggtgga ggaaaccacg gaaaggctgg ttcgcgaatt gtttccggtt attgctgaac
     4441 ggatcattag ggaagagatc gagaaactca agcagtgaga cccccgacat ccataaaaag
     4501 cgccggacaa gcgaagagaa taaatgaaga aaattgaacg cggacggaat ctccgtcgct
     4561 gtctgctgat cgccatgctt gcgatcagcc tgataactgg atgcgcaaag aagatccccc
     4621 attttctgac cccggaatat gacaaacggg gcgtgagact cattgccata ttgccggtgg
     4681 cgaatcaatc gaaaaatgca gaagcggcgg cgctgtttcg ggacaaggta ctggaggcgg
     4741 tatatttcca gggatatccc aaaatccctc tgaacgcgat cgacgagaaa ttatcacctt
     4801 tttttaacga ctatcggcgt cccactccgg aaaccatgcc gccccggcag gtaggtgaac
     4861 tgctgggcgt ggacgctgtc ctctatgtta ccctcaacga gtgcgatacc tcctttcttc
     4921 tcctttatgc cgccaccgaa gtcacggcca ctttcgattt gcggagcacc aagaccaacg
     4981 aaagcatctg gaataccaag tattctctta cggagaggaa ttttgagatc accagggatc
     5041 gcctccgcat gaagagttgt caagtcttcg agccggcaat ggaggagata attaccaagg
     5101 ctatggaaac cctccccctg ggaccggatg catgaagaaa aggtgaagct tcatgaagta
     5161 caagctgatt ttatctttct ttttcatagc catgctgacc tggttgatac cgggggattt
     5221 gttggccgga cggcccctga cggttaaggt ggataagggc gaggccgttg tcagccatct
     5281 cgaaggtgag gtccaggtcc ttccccaggg caaagatact tggcggattc taagggaaaa
     5341 ggccaacctt tgggggggcg atgagattgt tgtcgggagc aattcgagac tggagatcac
     5401 ccttgccggc gattcgcggc tccgtttcgc cgaggagacc cacttacatc tagaacgggc
     5461 cggggccggc aagtcggcgg atgtcaaggt tcaccttgtc gtgggccgga cctgggcaaa
     5521 ggtgagtaag acgatggggc taaagagaaa attcgagata tcctgcgaca acgctgttac
     5581 aggggtgcgg gggacgatct accggctgaa cgtgcaccgg gatgcctcgg cgctggtgcg
     5641 ggtatatgag ggtgaaatct ccgttaccgg cgcgacgaag gcgatggaga ccgagacgac
     5701 cctcggccct cccagcaaga ttaccggacc gcacaagatc actggtcccc acaaggtttc
     5761 ccgggaagag tggacgatga tcatccgttc catgcaacag gtgtatatcc gtgcggacgg
     5821 atcggcggat aagccacagg cgttttccgc ggaagaagac cgcgatgcct gggtggactg
     5881 gaaccgaagc agggacaggg tgctttgatg gatgcggggc ggatcaacat ccccaacacc
     5941 ctgacggttt tgcggatcat tctcgtgccc gcgatcgtca ttctcctcat gcagggtgat
     6001 tttttcaagg cgctgatcct ctttaccctg tccgggatca ccgacggtct ggatggcttc
     6061 ctggcccggg ttctccatca gcagacggaa ctgggggctt acctcgatcc gattgccgat
     6121 aaggccctga tggtgagttg cttcgtaacg ctgtccatca agcatgtcat tcctggctgg
     6181 ttgagcgtta tcgtcgtgag cagagactgc atcattctca tcggcatcgc aatgctggcc
     6241 atcatgtccg tatcctttca gatccgacct acgatgatca gtaaattgac cacggtattt
     6301 caacttttaa cgattttcgt cgtcctgctg gtccatacct tggcgatagt agtggatttt
     6361 aggatggttt tacttggatt atatattgtt acatcagcat tgactgttct ttcggggctg
     6421 aactacatct tcaaggggat acgtttcatc aattcggcat cgtaaaatat atagatttgc
     6481 cttgacataa aaggtttttc ctgttaagcg catggaccgt tcgcagataa ataagtttag
     6541 gcacgtagct cagggggaga gcgccatcct gacgcggtgg atgtccaggg ttcaaatccc
     6601 tgcgtgccta ccatgacatc gagagagggc atcagcgaaa tctgaacgtg gtgccctctc
     6661 tttaattcgt atcagggtga gaatgatgag ggagataaca attacctggc ctgatcgaca
     6721 gcagagcact ttgccgaacg gggttgccgt aaaagacgcc attgccgtct ggaagccgga
     6781 actgctggcg acgactgtgg cggtgaaaat caacggtaaa accgttgatc tgtcagctca
     6841 ggtttcggaa gacgccctgg tggaactcat cgatgtggcg tccaaggagg gcctgagcat
     6901 ccttcgccac agcgcctctc atatcatggc ccaggcggtg caggatatct ttcccggggc
     6961 caaggtaagc atcggtcctt ccatagagga tggtttttac tacgatttcg agtatgaaac
     7021 caccttcacg cccgaagatc tacagaaaat cgaacagcgg atgaaggaga tcgccgccac
     7081 cgatgccccc ttctcccgcc gggagctttc ccgggaggag gctgtggcct tgttccaaga
     7141 caagggagag acctacaagg tcgaactgat caacgatctc ccacccgatg tccggaccgt
     7201 gagtgtttac acccaggcgg gatatgaaga cctctgccgg ggtcctcatc tgccctcgac
     7261 gatcatggtc aaggccttcc gtctccttaa tgtggccggc gcctattggc ggggcgacga
     7321 gcgcaacagg atgctccaga ggatttacgg gacctgcttt gccacccagg aggcgctgga
     7381 cgaatacctg cggatgatgg gggaagccaa aaagcgggat caccgccgtc tgggccggga
     7441 gctggatcta ttccagatga acgacgaagc ggggccgggg ttgatcatct ttcatcccaa
     7501 ggggatgatg ctccgctatc tcatcgagga atgggaacgg caggagcacc tgaagcgggg
     7561 ctacgatatg gtcatgggtc cccagatcct caaagacgac ctctggaaaa ggtcgggaca
     7621 tttcgaccat tatcgggaaa acatgtattt caccgaggtg gagggtcagg gatacggcat
     7681 caagcccatg aactgcctgt cccacatgct tatctacaaa gcgaagatcc gcagttatcg
     7741 cgatctgccg ttacgttact tcgagctggg aacggtgcac cgtcatgaaa aaaccggggt
     7801 gttgcatgga ctcatgcggg ctcggcagtt cacccaggat gacgcccata tcctctgcac
     7861 ccccgaacaa ctcaacgagg agattcgggc gattgccgat ttcgtcgcct atgccatgaa
     7921 gatcttcggt tttgaatatg aagtagaact gagcaccagg ccggaaaaat ccatcggctc
     7981 cgacgcggac tgggaactgg cgacttcggc tctggaaggg gccttgaaag acggccgcat
     8041 gggttacgag atcaacgagg gtgacggggc gttttatgga ccaaagatcg acttcaagct
     8101 caaagacgcc ctaaaaagga aatggcagtg cgccaccatc cagtgcgact ttacccttcc
     8161 ggaacgtttc gacctgtcct atgtaggctc tgatggggaa aagcatcgcc ccgtcatgct
     8221 gcaccgggta attctcggtg ccattgagcg cttcatgggg gtcctgatcg aacattatgc
     8281 cggagctttt ccggtttggc tctcaccggt gcaggcgtcg atcctgacgg ttacggaaaa
     8341 gcaaattcct tacggagatc aggtccatca ggcgcttgta gatgccggta tccgggtcga
     8401 gaagagtttt accaacgata aactgggcta caagatcaga gaggcccaga tgcagaagat
     8461 gccttatatg ctggtcatcg gtgaccggga agtggcggaa cggaccattt ccccccgacg
     8521 acgcgacggg aaaaacatgg gggccatgac ggtgacggat tttatcgccc atgtccggga
     8581 agaggttagc aattatcgct aagctatgct aattttagga ggtgacgtat cgctaaggag
     8641 ttgaatatca acgaagaaat caaggcagtg caggtaaggc tcattgaaga gggcaggcag
     8701 ttgggtattc tgcccctcaa cgaagccttg gccatcgctg ccaaagccgg tctcgatctg
     8761 gtggagattg cgcccaatgc ggatccaccg gtctgccggg tcatggatta cggcaagtat
     8821 cgataccagc agagtaaaaa actccaggat gcgag
//
LOCUS       DAOSMF010000078         8711 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_9041_length_8711_cov_22.929413, whole genome shotgun sequence.
ACCESSION   DAOSMF010000078 DAOSMF010000000
VERSION     DAOSMF010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 8711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8711
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9041_length_8711_cov_22.929413"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /locus_tag="PLT63_07925"
     CDS             <1..797
                     /locus_tag="PLT63_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOD98689.1"
                     /translation="GTVGEPINPEAWNWYYNVIGRGECPIVDTWWQTETGGILITPLP
                     GAIDIKPGMATVPFMGVWPVLVDDEGKEFTETVASGALCIKMPWPGIMRGVYGDPKRF
                     QETYFEQFPGYYVTGDGCRRDKDGYYQITGRIDDVINVSGHRMGTAEVESALVAHAKV
                     AEAAVVGYPHEVKGQSIYAYVTLKSGIAKSDDLKKELVAHVRTLIGPIATPEKIQWAD
                     GLPKTRSGKIMRRILKKVAAGDIADLGDTTTLADPSVVDDIVKNRQ"
     gene            945..1718
                     /locus_tag="PLT63_07930"
     CDS             945..1718
                     /locus_tag="PLT63_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOD98690.1"
                     /translation="MNIVACVKQVPDTEAQIKVKGDGSGIEEGGIKWVMNPYDEFGVE
                     EALKLKEKLGGDVTIVSVGPARTMETVRTALAMGAEKGIQIDDPALYNTDAYATAEAL
                     AAAIKGIPYDIIFCGQRAIDDDSAQVGSILAELLGIPQVTFVTKLEINGTAVKATRPI
                     EGASVVVETSLPCVITATKGLNEPRYASLPGIMKAKKKPVDMKKAADLGVSGAAKAKV
                     VKFAPPPARPPGKIICADDTPEGKAAELAKLLREEAKVI"
     gene            1747..2718
                     /locus_tag="PLT63_07935"
     CDS             1747..2718
                     /locus_tag="PLT63_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOD98691.1"
                     /translation="MAKGVWIVAEQRDGALRKVSFELASTARKLADQLGDEVAAVLCG
                     SGVEAMAAELGKYGVDKVYVADNPALEPYTTDAHAAAVAKVVKENDPAILLVAASAQG
                     KDLAPSVVGKLATGMATDCTDVKIDGGKLVAVRPMYAGKCFGEVVIDTFPQMASLRPN
                     VFTIVECAKAGAVVKFDAGLGEAKTKVAEIQKEASGKIDVAEANIIVSGGRGMKGPEG
                     YGILEELAALLGAAVGASRAAVDAGWRPQSDQVGQTGKVVSPNLYIACGISGAIQHLA
                     GMSSSKYIAAINKDPEAPIFARADYGVVDDLFKVVPALTQEVKKLLA"
     gene            2854..5061
                     /locus_tag="PLT63_07940"
     CDS             2854..5061
                     /locus_tag="PLT63_07940"
                     /inference="COORDINATES: protein motif:HMM:NF014695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HOD98692.1"
                     /translation="MELSNFSIGHEGREVFWNAEHFEPILFALTFVALAIFAYGVYRR
                     WLMWTAIGKPEDRTDDRNERIKTLLKNGLLQLKTLADPYPGIMHALIFFGFVVLFFGA
                     AFDATEFHVAEPLGVPFLVGKFYLVFSFLMDLFGLAVLAGVLMALDRRYLTKPDRLGY
                     RGTPDNTADDAIVLLIIAGIIVTGFIIEALRIHVTWQQTPWEVWSFAGWTIAKAFSGV
                     DVGAAKAMHKWTWWIHTAFALSFIAYIPFSRLMHIITTPANYFMATRKPVGVLEPIKD
                     FENAESFGVGTLEEFTWKQIFDSDACTRCGRCQDGCPAYLTGKPLSPKKLVQDLKTYW
                     LEKAPGAVATRKEQLLAEGGDLEKINPKLKTLFQWLDKVDKVVDENLPKGPEPTEKAL
                     VGEVVDLDELWACTNCMYCMENCSASIEHVPKIVGMRQYKVLMEADFAPELQLTYRNM
                     ENNSNPWGVGSHLRADWAKELGVKTLAEDPNVEYLFYVGCAGSFDDRGKKVSVALAKI
                     LKAAGVSFGILGTEESCCGDSAMRGGNEYLYQTMAQANIEVMNGYGVKKVICTCPHGY
                     NALKKDYPNFGGNFEVYHHTEIIAELIEKGKIKIKNPVNDVRFTYHDSCFLGRYNQVY
                     DQPRKIIKAIPGARLVEMERNHGKSFCCGAGGARMWMEEHGDRINDARAKQAIDVGAQ
                     TIAVGCPFCLTMMSDGLKTHGKEEQMVALDLAEIVWKAMGLEAEPEPVEVCQA"
     gene            complement(5198..6289)
                     /locus_tag="PLT63_07945"
     CDS             complement(5198..6289)
                     /locus_tag="PLT63_07945"
                     /inference="COORDINATES: protein motif:HMM:NF028132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOD98693.1"
                     /translation="MNRYFESERGKFARYDSTLGWTGKENVQDDFLWMDVSHRVRQNR
                     YGCRGGEYPFGRTGKPRLLVLGDSYAWGFGVEEQQLFTSLLERNRNRDVEVVNLGVSG
                     YGTDQEYLLWKNKGHQWKPDQVLLLVTLWTDLWDNTISRQYGYEKPVFEPNASGELVL
                     KNTPVPREERHWSHGDRSNTERRSAWFSDGIGHSAVAAALVNFCVKSSTIRKLLEEHG
                     IIPIRQGGYGWEVQMYAPEGSDEMDKPWQVTFALIAKLAAEVKGQGGRLVVAVAPSPV
                     QVYPELWRERLGGRELGNKALDPSTPNKRLEAWCRQRGIQFIDLLTPLQKAGRTTPYL
                     YFPLNLHWTANGHQVVAEVLAQELSLFHP"
     gene            6690..8381
                     /locus_tag="PLT63_07950"
     CDS             6690..8381
                     /locus_tag="PLT63_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOD98694.1"
                     /translation="MAEENIYVKKQWLKSYEQGVPEAVAYEDLCMPEILDRTAKQFPD
                     RPALIFQGTTISFRGLQDMVDRFATCLTAFGIKKGDAVALLLPNIIPCVAAYFAVLKI
                     GAIAVMNNPLYSDPELDHQFNDSGSKVLITLDVLAPRMIALRPKTKVRQIVYTCIGDY
                     LPTLKRVLGRWLKKYPFADVPATADVYSWKECLAKYSPNPPAVKNSFEDIAMYQYTGG
                     TTGVSKGVMLTHANLSKQVQQLEAWFPKFTKGTEIMLGALPYFHVFGLSVSMNLSIHM
                     GWSQVLIPRPQPAPLLEAIRNFRPTFAPLVPTMYIGMLNHPDLKKTDMSCIKGAFSGS
                     APLPVEVIHAFEKATGAVIVEGFGMTETTPVTHVNPFAGGARKVGSIGVPISDTLCRI
                     VDLDSGTTDMPVGQPGELIIKGPQVMKGYKDKPEETAACLKDGWMFTGDIATMDEDGY
                     FYIVDRKKDMIISGGYNVYPRDIDEVYYEHPKVQEACSIGIPDAKRGENVKLFVVLKE
                     GETATQEELIEYGKTKLAAYKLPVEVEFRKELPKTNVGKILRKQLRAEEMEKRKK"
     gene            complement(8524..>8711)
                     /locus_tag="PLT63_07955"
     CDS             complement(8524..>8711)
                     /locus_tag="PLT63_07955"
                     /inference="COORDINATES: protein motif:HMM:NF024591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-aminobenzoate-CoA ligase"
                     /protein_id="HOD98695.1"
                     /translation="HPAVAAARVESKEDMMRGEIVKALIVRKPGVEADEKEILRHCRA
                     YLSSYKLPREVEFISNF"
BASE COUNT         1868 a         2526 c         2552 g         1765 t
ORIGIN      
        1 tcggcaccgt cggcgagccc atcaaccccg aggcctggaa ctggtactac aacgtcatcg
       61 gccggggcga atgccccatc gtcgatacgt ggtggcagac cgagacgggc ggcatcctca
      121 tcacccccct gcccggcgcc attgacatca agcccggtat ggccaccgtg cccttcatgg
      181 gcgtctggcc cgtcctcgtc gatgacgaag gcaaggaatt caccgagacg gtggcctccg
      241 gcgccctctg catcaagatg ccctggccgg gcatcatgag gggcgtctat ggggatccca
      301 agcgctttca ggagacctac ttcgaacagt tccccggcta ctatgtcacc ggcgacggct
      361 gccggaggga caaggacggc tactatcaga tcaccggccg catcgacgac gtcatcaacg
      421 tctccggtca ccgcatggga acggcggaag tggaaagcgc cctcgtggcc catgccaagg
      481 tcgccgaagc ggcggtcgtc ggttatcccc acgaagtcaa gggccagtcg atttacgcct
      541 atgtcaccct gaagagcggc atcgccaaga gcgacgacct gaagaaggaa ctcgtcgccc
      601 atgtccggac cctcatcggg cccatcgcca cgccggagaa gatccagtgg gccgatggcc
      661 tgcccaagac ccggagcggc aagatcatgc gccggatcct gaagaaggtg gccgcgggcg
      721 acatcgccga cctcggagac accacgaccc tggccgatcc gtccgtggtt gacgatatcg
      781 tgaagaaccg gcagtaaacg ggtaaaaact tttgtttccc cgcctccggc cccggtggtc
      841 agggggcggg gaaattcggt tgccgcgatc ctaataaatc tgacatcaat tatctgaccg
      901 atgtgcccgg cggcgccggg cgtcggttcg ggaaggagga caatgtgaat attgttgcat
      961 gtgtaaagca ggttccggat acggaagccc agatcaaggt caaaggtgac ggatctggca
     1021 tcgaagaggg cggcatcaaa tgggtcatga acccctacga tgaattcggc gtcgaagaag
     1081 ccctgaaact gaaagaaaaa ctcggcggcg atgtgaccat cgtttccgta ggtccggccc
     1141 ggaccatgga aacggtccgt accgccctgg ccatgggggc cgaaaagggc atccagatcg
     1201 atgatcccgc cctttacaac accgatgcct atgcgacggc cgaagccctg gcggcggcca
     1261 tcaaaggcat cccctacgat atcatcttct gcggccagcg ggccatcgac gatgactctg
     1321 cccaggtcgg gtccatcctg gccgagctcc tcggcatccc ccaggtgacc ttcgtgacaa
     1381 agctggagat caacgggacc gccgtcaagg ccacccggcc catcgaaggg gcgtccgtgg
     1441 tcgtcgagac ctccctgccc tgcgtcatca cggccaccaa gggcttgaac gaaccccgct
     1501 atgcatctct gcccggcatt atgaaggcga agaagaaacc cgtggacatg aagaaagcgg
     1561 ccgatctcgg cgtctccggc gctgccaagg ccaaagtcgt caagtttgcg ccgccccccg
     1621 cgcgtccccc cggaaagatc atctgcgcgg acgatacgcc ggaaggcaag gccgcggagc
     1681 tggcgaaact ccttcgggaa gaggcgaagg ttatctaatt atcaatgaac acggaggaat
     1741 gagaagatgg caaaaggtgt atggatagta gcagaacagc gagacggcgc cctgaggaag
     1801 gtcagcttcg agctggcgag cacggccagg aaactggcgg atcagcttgg cgacgaggtg
     1861 gccgccgtcc tgtgtggttc cggcgtcgag gccatggccg cggagctggg aaaatacggt
     1921 gtggataaag tctatgtggc ggacaatccc gccctggaac cctatacaac ggacgcccat
     1981 gccgcggcgg tggcgaaggt cgtgaaagag aacgatccgg ccatcctgct tgtggcggct
     2041 tccgcccagg gcaaggatct ggccccgagc gtcgtcggca aactggcgac gggaatggcc
     2101 acggattgta cggacgtcaa gatcgacggc ggcaaactgg tggccgtccg ccccatgtat
     2161 gccggcaagt gctttggcga agtggtaatc gatacgtttc cccagatggc ctccctgcgg
     2221 cctaacgtct tcacgatcgt ggagtgcgcc aaggcgggcg cggtagtgaa gttcgatgcc
     2281 gggctgggcg aagccaagac caaggtggcg gagatccaga aagaggcctc cggtaagatc
     2341 gacgtcgccg aggcgaacat catcgtctcc ggcggccgtg gtatgaaggg tccggaaggt
     2401 tacgggatcc tcgaagaact ggccgcgctc ctcggcgccg ccgtcggcgc ctcacgcgcc
     2461 gcggtggatg ccggctggcg tccccagtcc gaccaggtcg gccagaccgg caaggtcgtt
     2521 tccccgaacc tctacatcgc ctgcggtatc tccggtgcca tccagcatct cgccggaatg
     2581 agctcttcga agtacatcgc ggcgatcaac aaggatcccg aggccccgat cttcgcccgg
     2641 gccgactacg gggtggttga tgacctcttc aaggtcgttc ccgcgctgac ccaggaagtg
     2701 aaaaagctgt tggcttaaga atcataaccg gggacgccac caaggcgttc ccggtttttt
     2761 catggttgta caacctataa atgtcacgag actcttgaat gccggcggcg gaagatacgc
     2821 cggggccttc cggtctcgag tcagggagga tatatggagc tatctaattt ttcgatcggc
     2881 catgaagggc gggaggtatt ctggaatgcc gagcatttcg aacccatcct ctttgccttg
     2941 acctttgtcg ccttggccat ctttgcctat ggtgtgtatc ggcgatggct gatgtggacg
     3001 gccatcggca aaccggaaga ccggacggac gaccgcaacg aacggataaa aacgcttttg
     3061 aagaatgggc ttctccagct caagaccttg gccgatcctt atcccggcat catgcatgct
     3121 ttgatatttt ttggatttgt cgtgctcttt ttcggcgccg ccttcgatgc cacggaattt
     3181 cacgtagccg aacctttagg ggtcccgttc ctggtcggca agttctatct ggtcttttcc
     3241 ttcctcatgg atctcttcgg gctggcggtg ctggccgggg tcctgatggc tctcgaccgc
     3301 cgttatctga ccaaaccgga tcgcctcggc taccggggta cacccgataa caccgcggat
     3361 gacgccatcg tcctgcttat catcgccggg atcatcgtta cgggattcat tatcgaggcc
     3421 ctgcggatcc atgtcacgtg gcagcagacc ccctgggagg tttggtcctt tgccggctgg
     3481 accatcgcca aggccttctc cggcgttgac gtgggcgccg ccaaggcgat gcacaagtgg
     3541 acctggtgga tccacaccgc ctttgccctg agtttcatcg cctacatccc cttttcccgg
     3601 ctcatgcaca tcatcaccac cccggcgaac tacttcatgg cgaccaggaa gcccgtgggc
     3661 gttctggagc cgatcaagga ttttgaaaat gccgaatcct tcggggtggg gacgcttgag
     3721 gagtttacgt ggaagcagat cttcgattcc gatgcctgca cccggtgcgg gcgctgccag
     3781 gacggctgcc cggcctatct gaccggcaag cccctgtcgc cgaagaagct ggtccaggac
     3841 ctcaagacct attggctgga aaaggccccc ggcgctgtgg cgacaaggaa agagcagctt
     3901 ttggcggaag ggggagacct ggagaagatc aacccgaaat tgaaaaccct cttccagtgg
     3961 ctggataaag tggacaaggt ggtggacgaa aatctgccca agggtccgga accgacggaa
     4021 aaggccttgg tcggcgaggt cgttgatctc gacgaactct gggcttgcac caactgcatg
     4081 tactgtatgg aaaactgctc cgcctccatc gaacatgttc ccaagatcgt cgggatgcgg
     4141 cagtacaagg tcctcatgga ggcggatttc gcccccgaac tgcagttgac ctaccggaac
     4201 atggagaaca actccaaccc ctggggcgtg ggttctcacc tccgcgccga ctgggcgaag
     4261 gaactgggcg taaagaccct ggcggaagat cccaatgtgg aatacctctt ctatgtcggt
     4321 tgtgccggat cgttcgacga ccggggcaag aaggtctccg tagccctggc caagatcctc
     4381 aaggccgccg gggtaagctt cggcatcctc ggtaccgagg agagctgctg cggtgattcg
     4441 gccatgcgcg gtggcaacga gtacctctac cagaccatgg cgcaggccaa tatcgaggtg
     4501 atgaacggtt acggggtcaa gaaggtcatc tgcacctgcc cccacggcta caacgccctg
     4561 aagaaggatt atcccaactt cggcggcaat ttcgaggtct atcaccacac ggaaatcatc
     4621 gcggaactca tcgagaaggg caagatcaag atcaagaacc cggtgaacga cgtccgcttc
     4681 acctatcatg attcctgctt cctgggtcgt tacaaccagg tctatgacca gccccggaag
     4741 atcatcaagg ccatccccgg ggcccgcctc gtggagatgg aacgcaacca cggcaagagc
     4801 ttctgctgcg gcgccggcgg cgcccgcatg tggatggaag agcacggcga ccgcatcaac
     4861 gacgcccggg ccaaacaggc catcgacgtg ggcgctcaga ccatcgccgt cgggtgtcca
     4921 ttctgcctca ccatgatgag cgacggcctt aagacccacg gcaaggaaga gcagatggtc
     4981 gccctggatc tggcggaaat cgtctggaag gccatgggcc tcgaagcgga acccgagccg
     5041 gtggaggtct gtcaggcata atctctatcc ggagcggatg gatccacaag gatatcgata
     5101 ttccataagg gtagattgaa aaaaatttcg gaagcccccg gacagtcgga actgattgtc
     5161 cgggggcttc tctttctagg aacggtatgg caagcgatta aggatgaaac aatgataatt
     5221 cctgggcgag cacttcggcg actacctgat ggccattagc cgtccagtgt aaatttaggg
     5281 gaaaataaag atagggagtt gtccttcccg ctttttgaag aggcgtcagt aaatcgatga
     5341 attgaatccc tctttggcgg caccatgctt ccaggcgctt gttgggtgtc gacgggtcga
     5401 gcgccttatt tcctagttcc cgcccgccca gtctttcccg ccagagctcc ggatagacct
     5461 gcacggggct gggcgctacc gctacgacca atcttcctcc ctgtcctttt acctccgcag
     5521 ccagcttggc gatgagggcg aaggttacct gccagggctt gtccatttca tcggaccctt
     5581 ccggggcata catttggact tcccatccgt aaccgccttg ccggatcgga atgatcccgt
     5641 gttcctccaa caacttcctt atggttgagc tttttacgca aaaattgaca agcgccgcgg
     5701 ctaccgctga atgaccgatg ccgtcggaga accaggcgga ccggcgctcg gtgttgcttc
     5761 tatcgccatg ggaccagtga cgttcttctc gcgggacggg ggtatttttt agcaccagct
     5821 cccccgaggc attgggttca aagacgggtt tctcatagcc gtattgccgg gatatggtat
     5881 tatcccagag atcggtccac aaagttacca ataacaatac ctgatccggt ttccattgat
     5941 gtcccttgtt cttccagagc aagtattcct gatcggtgcc gtagccggaa acgccgaggt
     6001 tgacaacttc cacgtcccgg ttcctattcc tctccaacaa agaagtgaaa agctgttgtt
     6061 cttcgacacc gaatccccag gcatatgaat cgcccagcac caagaggcgc ggtttgccgg
     6121 tcctgccaaa ggggtattca ccgccccggc agccatagcg gttttgtcgg acacgatgag
     6181 atacatccat ccaaaggaaa tcgtcctgga cgttttcctt tcccgtccat cccaaggtcg
     6241 aatcatagcg tgcgaacttg ccccgttccg attcaaaata acgattcatc ttcaacagat
     6301 tgacgggggg aggagcaaga aaacggagga tgatttcccc gattatcagt cccccgacaa
     6361 ccccggcgat gatcagtgct gccttgatta ggatggatct ggccttttcc caagcggatg
     6421 ttccgcctgg cggtgctgac ggagatttcc cgcgcctttg tttacgacgg accgtatgct
     6481 tggcctcctt tacttaatgg gttcgagccg gatagattat gagggaatgt ttgtcaagac
     6541 aattttctcg atagcaaatt aatccacttt tttgtcttct ttgtgctata atgtcgactt
     6601 gtgggggcta atcgatctgt cgtatgtggg ggcgtgatgg tattatcgtt gccgtggaca
     6661 ataatttctc tgaaaggagt atggagatta tggcggaaga aaacatttac gtgaaaaaac
     6721 agtggttaaa gtcatatgaa cagggagttc ccgaagccgt ggcctatgaa gatctctgta
     6781 tgccggaaat tcttgatcgt acggccaaac agtttcctga tcggccggcc ttgatttttc
     6841 agggaacgac gatcagtttc cgggggttgc aggatatggt ggatcggttc gccacctgtc
     6901 tgacagcttt cgggatcaag aagggggacg ccgtcgccct gctgctgccc aacatcattc
     6961 cctgcgtggc ggcctacttc gccgtcctga agatcggcgc catcgccgtc atgaacaacc
     7021 ccctctattc cgatccggaa ctcgaccatc agttcaacga ttccggttcc aaggtcttga
     7081 tcaccctgga tgtattggcg cccaggatga ttgccctccg tcccaagacc aaggtcaggc
     7141 agatcgtcta tacctgcatc ggcgactatc ttcctacctt gaaaagggtg ctcggccggt
     7201 ggctgaagaa ataccccttt gccgatgtcc cggcgacggc cgatgtctat tcctggaagg
     7261 aatgtctggc aaaatattcc ccgaaccccc cggcggtgaa gaacagcttt gaagatatag
     7321 ccatgtacca gtacacgggg gggacgacgg gggtgtccaa gggcgtcatg ttgacccatg
     7381 cgaacctgag caagcaggtc cagcagttgg aggcgtggtt tcccaagttc accaaaggca
     7441 cggagatcat gctgggggcg ctgccctatt ttcacgtctt cgggctctcc gtctccatga
     7501 acctctccat ccacatgggc tggtcgcagg ttctcatccc ccgtccccag cccgcgccgc
     7561 tcctcgaggc gatccgcaat ttccgcccca ccttcgcccc gctggtgccg acgatgtaca
     7621 tcgggatgct caaccatccc gacctgaaga agaccgacat gagctgcatc aagggggcct
     7681 tctccgggag cgcgccgctg cccgtggagg tgatccatgc ctttgaaaag gctaccggtg
     7741 cggtgatcgt cgaaggtttc gggatgacgg aaacgacacc ggtcacccac gtgaatccct
     7801 ttgccggcgg cgcccggaag gtggggagca tcggcgttcc catctcggat accctgtgcc
     7861 gcatcgtcga tctcgatagc gggaccacgg acatgcccgt cggccagccg ggggaactga
     7921 tcatcaaggg tccccaggtc atgaagggtt acaaggacaa gccggaggag accgccgcct
     7981 gtctgaaaga cggctggatg tttaccggcg acatcgccac catggacgaa gacggctact
     8041 tctacatcgt ggatcgcaaa aaggacatga tcatttccgg tggctacaac gtctatcccc
     8101 gggatatcga cgaggtttac tacgagcatc cgaaggtgca ggaggcctgc tccatcggca
     8161 tccccgacgc caaacgcggt gaaaacgtca aactgttcgt ggtcttgaag gaaggggaga
     8221 cggctaccca ggaagagctg atcgaatacg gcaagacgaa gctcgccgcc tacaaactgc
     8281 ccgtcgaggt ggagttccgc aaggagctgc ccaagaccaa tgtgggcaag atccttcgca
     8341 aacagctccg ggcggaggag atggagaaac ggaaaaaata ggccgcaggc agtaagcaat
     8401 tggtggcctg gcagccatcc tggatagtga atgaactcgg ggagcggatt taactcgtca
     8461 tctgttcccc gagttcattt tttacctcgg gaaatcgccg tcgtggtttt ttttcaaaaa
     8521 ctgttaaaag ttgctgatga attccacctc ccgggggagc ttgtaggagg atagataggc
     8581 ccggcagtgg cggaggatct ccttctcgtc ggcttccacg cccggtttcc ggacgatcag
     8641 ggccttgacg atctcccccc gcatcatgtc ctccttgctt tccacccggg ccgccgccac
     8701 ggcggggtgc a
//
LOCUS       DAOSMF010000079         8671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_9090_length_8671_cov_23.629875, whole genome shotgun sequence.
ACCESSION   DAOSMF010000079 DAOSMF010000000
VERSION     DAOSMF010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 8671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8671
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9090_length_8671_cov_23.629875"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..2739)
                     /locus_tag="PLT63_07960"
     CDS             complement(34..2739)
                     /locus_tag="PLT63_07960"
                     /inference="COORDINATES: protein motif:HMM:NF015644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HOD98696.1"
                     /translation="MKKANLIFIAVLLVILVVAPLLSSLLNLYIDWLFFVETGYTAVF
                     TKTLTTQIAAGFLFGLLFLGAALINLFFARRVEFSRPDQYNISGTPITINLAPLRTIQ
                     RAVTFFILLVVTFMMGKWGATLWSEILLFTNALTVGQNDPVFGRDIGFYLFQYPFFES
                     LKQFVDFTLILCAILAGIVYFLGGGILVNQRQITIDPRVKRHLGILIGLMIINMGPGF
                     YLEALRMLYAEHGVIFGASYTDVHAKLAAYRLLAFLAPAAGAVLIAGMFKRQRRLTMI
                     ALGALFAVYFIGIMIYPALLQKFKVAPNEMVMETPYIENNIRMTRLGYDLDRIEEIPF
                     DVDYNLTGRDIDKNDATIRNIRLWDHSPLLRTYSQLQQIRTYYRFQDVDNDRYTVNGQ
                     YLQVMLSPRELSYADLPSKNWINERLIFTHGNGITLGPVSRISREGLPEFFIKDIPPA
                     GSTDLKVQRPEIYYGEMSNDYVIVKTKIPEFSYPTPEGGNIYTSYGGAGGVKTGSLTR
                     RLLFSLKYQTEKIFFSSDITADSKILFFRNVKERFQKIAPFLLFDHDPYLVITDDGRL
                     VWMVDAYTVSSRLPYSKPSVRRINYVRNAVKATLDAYDGTVTYYVSDPSDKIIQVYRR
                     IFPALFKDLAVMPEDLKRHIRYPQWLLQLQASVYAAYHMTDPKIFYNKENLWEIPVFE
                     EKTMQPYYTIMKLPSEKREEYILLLPYTPAKRDNLAAWLAARCDAPHYGKLVVYTFPR
                     DRLVFGPKQVDARINQDSYISQQLTLWSQRGSRVIRGSLLVIPVERSLLYVQPLYLAA
                     SDAVGLPELRRVIVAYENEVVMEENLELALQRLFGSRKAGAVTTTTAGAPAAAAAPGR
                     ASVKELAAEAMKTYQRSVDMQRQGNWAGYGEEIRKLEQLLKKMAN"
     assembly_gap    2818..2917
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2940..4148)
                     /locus_tag="PLT63_07965"
     CDS             complement(2940..4148)
                     /locus_tag="PLT63_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HOD98697.1"
                     /translation="MADFWQHGMITTLQKLKDRPVAAIEEELRIIARRRNMTLLLPAL
                     YSEFETPAIPKIINELKKVNYLYQIVLSLDRADREQFQRVREIMAALPTATRVVWHDG
                     PRMQKLYRVLRENDFTLDMPGKGRSVWMTMGYILSDRSVYAIALHDSDIVNYEREILA
                     RLLYPVAHPAISLEFSKGYYARVTDRLYGRVVRLFYTPLIRTLRRVLGFNPFLSYLDN
                     FRYALSGEFALISSLARDLRISPTWGLEVSLLSEVYQRTSVNRICQVEVIESYEHKHQ
                     LLDKEKPDQGLIRMANEIAQTLFRVLSQDGVVMSEAFYRTLLTAYIQESRIAIEKYHA
                     LTLLNGIHYDRHSEIEAVEAFVGSLRTAIDEFISDPVGVPMLPAWVRVEAAVPDFLDR
                     LQEAVEADNQ"
     gene            complement(4141..5403)
                     /locus_tag="PLT63_07970"
     CDS             complement(4141..5403)
                     /locus_tag="PLT63_07970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOD98698.1"
                     /translation="MEPTRFTTTLREHVRRKIELLRETDIVVGVPAYYSAPYIVHVIQ
                     TIIKGLNEYYPGYRALVMISDGGSTDDTREVANLVTVDSFHINKIVTIYRGMPGKGTG
                     LKAIFEAADFLKAKAVAVFDSDLISITPEWIKNVLEPIFLGYDYVAPDYRRYKLDGTI
                     TNTIAYNLTRALYGCRIRQPIGGDFGISAPLVHRYLQEEVWETDVARFGIDIWMTTTA
                     IVGGYRICQTRLGAKIHGEKDPTADLGPMFRQVVGTTFQLMDQNERYWRNVTGSQPVP
                     SFGVYTGSQPKEFIIDHDALIDYFRTGYRNFRGVWRNVIMEGDYEIIRQLARNGARDR
                     FRMPIETWVRMVYCYAGAFHATPRQRFKLLDTLIPLYYGRVASLVNELKDKDGNAAEE
                     HFETQAFMFETLKDHLRAVWTRKEIRNG"
     gene            complement(5403..6722)
                     /locus_tag="PLT63_07975"
     CDS             complement(5403..6722)
                     /locus_tag="PLT63_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HOD98699.1"
                     /translation="MDLRTPAVEIYRDAVASVLPERLLAAVLQAGPEGLRIGGRRYPW
                     PVRRKLWLLGSGKASLAMARAALKIIGRERLAGGLIISSYDDGSLGELPVAVGAHPLP
                     DERSLAAAERLMEIMGSLMREDLFIYFLSGGASALLEKPLAPITGDDLREVYRLLMTR
                     SIPIEEMNVVRKHLSMVKGGQLAARTKARGAVLVMSDVIGDDLEAIGSAPLYRDRSTY
                     REAHRILDYHGLWPLLPASVRAVVEAGGDGALAETPKEAPRRIAHHVIAGNAILLRQA
                     RRRAAALGMEARIVTSSLRGEAREVAKVLMAIGEEEQKRRRRSSRTLCLLWGGETTVT
                     LRGRGRGGRNQELCLAFLRELRSRRGLCLLSAGTDGIDGNSDAAGAVVDAASHRTAAG
                     RGLSLEDYLQANNAYEFFRQTGDLIITGPTGVNVMDLTLLLVAREKP"
     gene            complement(6697..7566)
                     /locus_tag="PLT63_07980"
     CDS             complement(6697..7566)
                     /locus_tag="PLT63_07980"
                     /inference="COORDINATES: protein motif:HMM:TIGR01486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HOD98700.1"
                     /translation="MPAPTLPGGRTIVFTDLDGSLLDHHSYSHAGAAPVLARIREEGI
                     PLIIVTSKTRAEVVEIQREFGNADPFIVENGGAAYFPRSGPYRFLAAGEEAAGYRRIG
                     FGRSYGEIRDFLAAFKSEEKKREIIKGFGDMELEDIMALTGLDAVRAGAAKRREFSEP
                     FLIAGETDAAAACLRELAAEARRRGFEITRGGRFHHLLGAGQDKGRAVAATVKTLRGL
                     AGGKITTVGVGDSDNDLTMLAAVDIPVLIPHPHRGFPAGTPAGAVRATRPGSEGWNEA
                     VGKALDGLTNTGR"
     gene            complement(8036..>8671)
                     /gene="pal"
                     /locus_tag="PLT63_07985"
     CDS             complement(8036..>8671)
                     /gene="pal"
                     /locus_tag="PLT63_07985"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HOD98701.1"
                     /translation="KNVMAAINKIKADYGDRVCIYTIHVDNDAKGNILAQNIIKEAGC
                     GEAVLAESLKDPEAMKEFVKRIFLDAAPVKAAAPAPVQEQAQPAAAAAAPAVAAAFDL
                     ANIHFDFDKSSLRPEDRNILKGHAEWLMKNKDANIIVEGHCDERGTTEYNLALGERRA
                     TETKKYLVELGVPEGRIKTISYGEERPLDPGHTEAAWAKNRRAQFVLNKNP"
BASE COUNT         1552 a         2482 c         2814 g         1723 t
ORIGIN      
        1 ccagccatag cgcatttttt tcgtcaaggc aatctaattg gccatcttct tcagcagttg
       61 ttccagcttc cggatctctt cgccgtaacc ggcccagttg ccctggcgct gcatatccac
      121 ggaccgctgg taggtcttca tcgcctcggc cgccagttcc ttcacggagg cgcgtccggg
      181 agcggcggcc gccgccgggg cgccggccgt tgtcgtcgtc accgccccgg ccttccggct
      241 gccgaagagg cgctggaggg ccagttcgag attctcctcc atgaccacct cgttttcgta
      301 ggccacgatg acccggcgca gctccggaag gcccacggcg tccgacgcgg ccaggtagag
      361 gggctgcacg tagaggaggg accgctccac ggggatgacg aggaggctcc cccggatgac
      421 ccgggagccg cgctggctcc agagggtgag ctgctgggag atataggaat cctggttgat
      481 ccgggcgtca acctgcttgg gaccgaagac caggcgatcc cgggggaagg tatagacgac
      541 gagcttgccg taatgagggg cgtcgcaccg cgcggccagc cacgcggcga ggttgtcccg
      601 cttcgccggt gtgtagggaa gcaggaggat gtactcttcc cgcttctcgc tggggagctt
      661 catgatggtg tagtacggct gcatcgtttt ctcctcgaag acgggtatct cccagagatt
      721 ctccttgttg tagaagatct tcgggtccgt catgtgatag gcggcataaa ctgaggcctg
      781 caactggagg agccactggg ggtagcggat atggcgtttt agatcctccg gcatgacggc
      841 caggtccttg aagagggccg ggaatatccg ccggtaaacc tggatgatct tgtccgaggg
      901 gtcgctcacg tagtaggtta ccgtgccgtc gtaggcgtcg agggtggcct tgacggcatt
      961 gcgtacgtag ttgatccgcc ggaccgaggg tttggagtag ggcaggcgcg acgataccgt
     1021 ataggcgtcc accatccaaa ccagccggcc gtcgtcggtg atcaccaggt agggatcgtg
     1081 gtcgaagagg agaaaaggcg cgatcttctg gaatctttcc ttgacgttgc ggaagaagag
     1141 gatcttactg tcggcggtga tgtcggagga gaagaagatc ttttccgtct ggtatttcag
     1201 ggagaagagg agacggcggg tcaatgagcc cgtcttcacg ccgccggcgc cgccgtagga
     1261 ggtatagatg ttgcctcctt ccggggtggg atagctgaac tcggggatct tggtcttgac
     1321 gatcacgtaa tcgttggaca tctctccata atagatctcc ggtcgctgca ccttgagatc
     1381 cgtggagccg gccggcggga tgtccttgat gaagaattcc ggcagaccct cccggctgat
     1441 ccggcttacc ggacccaggg tgatgccgtt gccgtgggtg aagatcaggc gttcgttgat
     1501 ccagtttttg ctgggcagat ctgcgtagga gagttcccgg ggcgacagca tgacctggag
     1561 atactgaccg ttcacggtgt agcggtcgtt gtccacgtcc tgaaaccggt agtaggtccg
     1621 gatctgctgc aactggctgt aagtgcgcag gagcggggag tgatcccaga ggcggatgtt
     1681 ccggatcgtc gcgtcgtttt tgtcgatgtc ccgtcccgtg agattgtagt ccacgtcgaa
     1741 ggggatctcc tcgatgcggt ccagatcgta gcccaggcgg gtcatgcgga tattgttttc
     1801 gatgtagggg gtttccatga ccatctcgtt gggggcgacc ttgaacttct ggaggagggc
     1861 cggatagatc atgatgccga taaaatagac ggcgaacagg gcgcccagag cgatcatggt
     1921 caggcgccgc tgccttttga acatcccggc gatcaggacc gccccggctg ccggggccag
     1981 gaaggcaagg aggcggtagg ccgcgagctt ggcatggaca tccgtatagc tcgccccgaa
     2041 gatcacgccg tgttccgcgt agagcatcct cagggcctcg agatagaagc ccggacccat
     2101 gttgatgatc attaggccga tgagaatgcc caggtgtcgc ttgacccggg gatcgatcgt
     2161 tatctgccgc tggttgacga gaatcccgcc gcccaggaaa tagacgatcc ccgccaggat
     2221 ggcgcagagg atgagggtaa aatcgacgaa ctgcttgagg gattcgaaaa agggatactg
     2281 gaagagataa aagccgatgt ccctcccgaa gaccggatcg ttttgaccga cggtcagggc
     2341 attggtgaac aataggattt cggaccacag ggtggcgccc cattttccca tcatgaaggt
     2401 gacgaccagg aggatgaaga aggtaaccgc ccgctggatg gtccggaggg gcgcgaggtt
     2461 gatcgtgatg ggggtgccgc tgatgttgta ctgatccggg cgggagaatt cgacgcgccg
     2521 ggcgaagaag agattgatca aggcggcgcc caggaacagg aggccgaaaa ggaagcctgc
     2581 ggcgatctgg gtggtcagcg ttttggtgaa gacggcggtg tagcccgttt cgacaaagaa
     2641 gagccagtcg atgtagaggt tgagcaggga agacaagagc ggcgccacga cgaggatgac
     2701 cagcaggacg gcgataaaga tcagattggc ctttttcatt tgcactcctt caaggtttgt
     2761 tcctgttcat aatcgggaac gatttataaa gcaagcaccg gcttgccggc gggatgcnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnccg gcgggatgcg cgccgaaatt
     2941 cactgattgt ccgcttccac ggcctcctgg agccggtcca agaaatccgg cacggctgcc
     3001 tccacccgga cccaggcggg gagcatggga acccccacgg ggtcgctgat gaattcgtcg
     3061 atggccgtcc ggagggagcc gacgaaggcc tccaccgctt cgatctcgct gtgccggtcg
     3121 tagtggatgc cgtttaagag ggtcagggcg tggtactttt cgatggcgat ccgtgattcc
     3181 tggatatagg ccgtaagcag ggtgcggtag aaggcctcgc tcatcaccac cccgtcctgg
     3241 gaaaggaccc ggaagagggt ctgggcgatc tcgttggcca tcctgatcag cccctggtcg
     3301 ggcttctcct tgtccaggag ctggtgcttg tgctcgtagg attcgatgac ctccacctgg
     3361 cagatgcggt ttaccgaagt gcgctggtaa acctcgctca gcagcgatac ctccagcccc
     3421 caggtcgggg agatccgcag gtcccgggcc agactgctga tgagggcgaa ttcccccgag
     3481 agggcgtagc ggaaattgtc caggtaggac agaaaggggt tgaagcccag gacccggcgg
     3541 agcgtccgga tcaggggcgt atagaacagc ctgaccaccc ggccgtagag gcggtccgtc
     3601 acccgggcgt aatagccttt gctgaattca aggctgatgg ccggatgggc gacgggatac
     3661 aggaggcggg ccaggatctc ccgctcgtag ttgacgatgt cgctgtcgtg aagggcgatg
     3721 gcgtaaacgg agcggtcgga aaggatatag cccatggtca tccagaccga tctacccttg
     3781 ccgggcatat cgagggtaaa gtcgttctcc ctgagaaccc ggtagagttt ctgcatccgg
     3841 ggcccgtcgt gccagacgac cctggtggcc gtgggcaggg cggccatgat ttcccggacc
     3901 cgctggaatt gctcccggtc ggcccggtcg agggaaagga cgatttggta gagataattc
     3961 acctttttca attcatttat gatcttcggg atggccggtg tttcgaattc cgagtagagg
     4021 gccggcagca gcagggtcat attccgccgc cgggcgatga tgcgcaactc ctcttcgatg
     4081 gccgccacgg ggcgatcctt gagtttttgc agggtggtga tcatgccgtg ttgccaaaaa
     4141 tcagccattt ctaatctcct ttcttgtcca aacggcccgc aggtgatctt tcagggtctc
     4201 gaacatgaag gcctgggttt caaaatgttc ttccgcggcg ttgccgtcct tgtccttgag
     4261 ctcgtttaca agcgaggcga cccgtccgta gtacaggggg atgagggtgt ccagaagctt
     4321 gaagcgctgg cggggagtgg cgtgaaaggc cccggcgtaa caatagacca tccggaccca
     4381 ggtttcgatg ggcatccgga acctgtcccg ggcgccgttg cgggccaact gccggatgat
     4441 ttcatagtcc ccctccatga ttacgttccg ccagacgccc cggaaattcc ggtagcccgt
     4501 ccggaagtaa tcgatcaggg cgtcatgatc gattatgaat tccttcggct gggacccggt
     4561 gtaaaccccg aagcttggga cgggctggga gccagtgacg tttcgccagt agcgctcgtt
     4621 ttggtccata agctggaacg tggtgccgac gacctggcga aacatgggac ccagatcggc
     4681 ggtgggatcc ttttccccgt ggatcttggc gcccagtctc gtctgacaga tccggtagcc
     4741 ccccacgata gccgtggtgg tcatccagat gtcgatgccg aagcgggcca cgtcggtttc
     4801 ccagacctcc tcctggagat agcgatggac gagcggcgcg gagatgccga aatcaccgcc
     4861 gatgggctgg cggatccggc agccgtagag ggcccgggtc aggttgtagg cgatggtatt
     4921 ggtgatggtg ccgtcgagtt tgtagcgccg gtaatccggg gctacatagt cgtaacccag
     4981 aaagatgggt tccaggacgt tcttgatcca ttccggggtg atggagatga gatcggaatc
     5041 gaagacggcc accgccttgg ccttgaggaa atcggcggcc tcgaagatcg ccttgaggcc
     5101 ggtgcccttg ccgggcatgc cccggtagat ggtgacgatc ttgttgatat ggaaggaatc
     5161 gacggtgacc agattggcca cctcccgggt atcgtccgtg gagccgccgt cggagatcat
     5221 gaccagggcc cggtatcccg gatagtactc gttgagcccc ttgatgatgg tctgaatgac
     5281 gtggacgatg tagggggcac tgtaataagc ggggacgccc acgacgatgt ccgtctcccg
     5341 gagaagttcg atcttccggc gcacatgctc ccggagggtg gtggtgaatc gagttggttc
     5401 cattacggtt tctcccttgc taccagcagc agggtcaagt ccatgacatt gacccccgtg
     5461 gggccggtga tgatgagatc accggtctgc cggaaaaatt cgtaggcgtt gttggcctgc
     5521 agataatctt ccagggacag tccccgcccg gcggcggtgc ggtggctggc cgcgtccacc
     5581 acggcgccgg cggcgtcgga attgccgtca atgccgtccg tcccggcgct caggaggcat
     5641 aacccccgcc gggagcggag ttccctgagg aacgccaggc agagctcctg atttcttccc
     5701 ccgcggccgc ggccccggag ggtgacggtg gtctcgccgc cccagaggag gcacagggtc
     5761 ctgctggagc ggcgccgccg cttttgctcc tcttccccga tagccatgag aaccttggcg
     5821 acctcccggg cctcaccgcg gagggaagag gtgacgatcc gcgcctccat gcccaaggcc
     5881 gccgctcgcc gccgggcctg ccggagcagg atggcgttgc cggcgatgac gtggtgggcg
     5941 atgcgtcgcg gcgcctcttt aggggtctcc gccagcgcgc cgtcgccgcc cgcctccacc
     6001 accgcgcgga ccgatgcggg cagcaggggc cagaggccgt ggtaatccag gatgcggtgc
     6061 gcctcccggt atgtggaccg gtcgcggtac aggggggcgg agccgatggc ctccaggtcg
     6121 tcgccgatga cgtcggacat gaccagtacc gccccccggg ccttggtgcg ggccgcgagc
     6181 tggccgccct tgaccatgga caggtgtttc cggaccacat tcatctcctc gatggggatg
     6241 ctacgggtca tgaggagccg ataaacctcc cggaggtcgt cgcccgtaat gggggccagc
     6301 ggtttttcca ggagggcgga ggcccccccg gaaaggaaat agatgaacaa gtcctcccgc
     6361 atgagggagc ccatgatctc cataagcctt tccgcggccg ccaggctccg ttcgtccggg
     6421 aggggatggg cgcccacggc caccggcagc tccccgaggg aaccgtcgtc atagctggag
     6481 atgatgagac ccccggccag ccgttcccgg ccgatgatct tcagggcggc ccgggccatg
     6541 gccagggagg ccttgccgct ccccagaagc cagagtttcc gccggaccgg ccaggggtag
     6601 cggcggccgc cgatgcggag cccctccgga cccgcctgga ggaccgccgc cagcagccgt
     6661 tccggaagaa cggatgccac cgcgtcccga tatatctcaa cggccggtgt tcgtaagtcc
     6721 atccaatgcc tttcctaccg cttcgttcca tccctcgctt cccggtcgcg tcgcccggac
     6781 ggccccggcc ggcgtcccgg cgggaaatcc ccggtgcggg tgggggatga ggacggggat
     6841 gtccaccgcg gcgagcatcg tcaaatcgtt gtcgctatcc ccaacgccga cggtcgttat
     6901 cttcccgccg gcaaggccgc gcagggtttt cacagtcgcg gccacggccc ggcccttgtc
     6961 ctgtcccgcc cccaggagat gatggaacct cccgccccgg gtgatctcga agccccggcg
     7021 ccgggcctcc gccgccaact cccggaggca ggcggccgcg gcatcggttt ccccggcgat
     7081 caggaatggt tcgctgaatt cccgccgttt ggccgccccg gcccggactg cgtcaaggcc
     7141 ggtcaacgcc atgatgtcct ccagctccat atcgccgaaa ccctttatta tttcacgttt
     7201 tttctcttcg ctcttgaatg cggcgagaaa atcccggatc tctccgtagc ttctgccgaa
     7261 cccgatccgc cggtagcctg ccgcctcctc ccccgccgca aggaaacgat agggaccgct
     7321 tcgggggaaa tatgccgccc cgccgttttc cacgatgaag gggtcggcgt tgccgaactc
     7381 ccgctggatc tccaccacct cggcccgggt cttgctcgtg acgatgatca gcggtatccc
     7441 ttcctccctg atccgggcca ggaccggggc cgccccggcg tgggaataac tgtggtgatc
     7501 cagaagggac ccgtcgagat cggtgaagac gatcgtccgt cccccgggga gagtcggcgc
     7561 gggcatcagg ggcgataccc ctgcgatccc ttgtccgacc cgggtaattc gggggcggac
     7621 gctttcgaga caaggtgaaa atccatggct ttcgttttct cgggacgact tgtcccggga
     7681 atttcctacg tttcatgccg gcacggcgtt tgcgggtaac accggaaatg gttgagctgt
     7741 cttttacttc atgcctggcc cgtctcggca tggggggttt tatgcgtgct taggattccg
     7801 gcctttttag taacgacggg gaaaaagacg gtcaagcatg gtcgcgagag atggaaagtg
     7861 ccttcgggcg tgcggggcgg gacgccgata acacatcgtc atatttcgct cggcacgaaa
     7921 acaaggtagc agaaaaatgg ccgtctgtaa aggacagagg gaaaagaaag gcctttcccg
     7981 gcctgctgca tccgggaaag gccttgatcg cggctgttgt tagaccttgt cgcggttacg
     8041 gattcttgtt caggacgaac tgcgcccggc ggtttttggc ccaggccgcc tcggtgtggc
     8101 cgggatccag gggacgctct tcgccgtagc tgatggtttt gatccggcct tccggaacac
     8161 ccagctcgac caggtatttc ttcgtttccg tggcccggcg ttcgccaagg gcgaggttgt
     8221 attccgtcgt gccgcgttcg tcgcagtgac cttccacaat gatgttggcg tctttattct
     8281 tcatgagcca ctcggcatgt cccttgagga tattgcggtc ctcgggacgg agcgacgact
     8341 tgtcgaaatc gaaatggata ttggccagat cgaaggctgc cgccactgcg ggcgccgccg
     8401 ccgccgcggc cggctgtgcc tgctcctgca ccggcgccgg tgccgccgcc ttaaccgggg
     8461 ccgcgtcgag gaatatccgc tttacgaatt ctttcatggc ctcgggatcc tttaagcttt
     8521 cggcgagcac cgcttcgccg cagccggctt ccttgatgat attttgagca aggatgttgc
     8581 ccttggcatc attgtccaca tggatcgtat agatgcatac ccggtcgccg tagtccgcct
     8641 tgatcttgtt gatggcggcc atgacgtttt t
//
LOCUS       DAOSMF010000080         8336 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_9552_length_8336_cov_23.407439, whole genome shotgun sequence.
ACCESSION   DAOSMF010000080 DAOSMF010000000
VERSION     DAOSMF010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 8336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8336
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9552_length_8336_cov_23.407439"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2177)
                     /gene="alaS"
                     /locus_tag="PLT63_07990"
     CDS             complement(<1..2177)
                     /gene="alaS"
                     /locus_tag="PLT63_07990"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOD98702.1"
                     /translation="MTMTASEIREQFLRFFEGHGHTIVPSSSLIPKDDPTLLFTNSGM
                     VQFKNCFLGLEDRGYSRAASSQKSVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKKESIAWGWEFLTVNMGLPKDKLWITIYKDDDEAFEIWNKQMGVPAERIVRMGMESN
                     FWMMGETGPCGPCSEILYDQGPSVGCGRPECSVECDCDRHLEIWNHVFTQFDRDQDGN
                     FQPLPKPNIDTGMGLERLAAIVQGVQSNYDTDLFTPIIAGIAGIAGRAYGANAEADVS
                     MRVIADHGRAVTFLIGDGVLPSNEGRGYVLRRILRRAARHGKLLGIDRPFLPEVVSLV
                     VTVMQEAYPDLIDKESYIRKVVVNEEQRFIDTLDTGLRILGEEVALLKKEGRQVIPGD
                     VVFKLYDTFGFPVDLTADIVRKDGLTLDMEGFEAAMEAQREKARESWKGSGEQAVADS
                     YKKLSVRGVTTAFIGYHGVTEAAAVVTAILKKDAEVEELVAGENGEIFVDETPFYGEK
                     GGQVGDTGVIAGDGYLFEVWDTQCPTDTLITHIGKMKEGKIRVGDTVQLRVDETSRRA
                     TEAHHSGTHVLNAALKKVLGDHIKQAGSSVTPERFRFDFTHFSRIEAEELDAIENIAN
                     EYIRRNAEVNTRVLPKEEAMKTGAAAVFDEKYSDNVRVVKMGDFSMELCGGTHVNRTG
                     DIGALKVVGESAVAAGVRRIEAVTGGEAIKYFKAVEAELKKSAG"
     gene            complement(2158..>2638)
                     /locus_tag="PLT63_07995"
     CDS             complement(2158..>2638)
                     /locus_tag="PLT63_07995"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HOD98703.1"
                     /translation="LARAKQVCWRLLARRDHGEEELARKLTKKGFPGEVVTGTIDYFR
                     GLGYLNDKLFAAGLARRLAEDKGLGDRRLHSLLVQKGISASLAEEAITAIRQEWSEEQ
                     AVRSLIKKKTKSRPLREDPQGKQRLFRYLLGRGFAPGLIYELINRAEEEDGHDDDGK"
     assembly_gap    2639..2738
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2752..3765)
                     /gene="recA"
                     /locus_tag="PLT63_08000"
     CDS             complement(2752..3765)
                     /gene="recA"
                     /locus_tag="PLT63_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOD98704.1"
                     /translation="MGVDMDRNKAVDLAISQIERQFGKGAIMKLGGSEKIDVPVIPTG
                     TLSLDIALGVGGVPRGRIIEIYGPESGGKTTLALHIVAEAQRQNGLAAFIDAEHALDV
                     GYARKIGVNTDELLIAQPDTGEQALEIAETLVRSGALDVLVVDSVAALVPRAELEGEM
                     GDAQMGLQARLMSQALRKLTGSISKSRTTVIFINQLRMKIGVFFGSPETTTGGNALKF
                     YASMRLDIRKVSSLKQGQDVIGMRTRVKVVKNKVAPPFRETEFDIIFGEGISREGDLL
                     DLAAENAIIEKSGAWYSYKGERLGQGRDNTRNFLKEHPEVLQEVESGVREKFGLKAAA
                     VAE"
     assembly_gap    3990..4089
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4090..5327)
                     /locus_tag="PLT63_08005"
     CDS             complement(<4090..5327)
                     /locus_tag="PLT63_08005"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HOD98705.1"
                     /translation="MHIGIITIGNELTSGRIQDANTAFLARQFHLQGWQVVASLSVGD
                     EEEMIAAALAFILPRAETVIVTGGLGPTADDITTAALAHIFGLPLITDEETLQHIRGI
                     FTRYGLKWTPNNAKQAVFPQGAEIIANPTGTAPGFSLRREGKVIAVIPGVPAEVRRMV
                     PAGVVPLLKRAFPGHARHTAVRTLKTFGLSEAAVDQAVAGVELAGVEIGFYPRFPENH
                     IVLSVRDEHDEAAARARLAAVEEEVRRRLDRYIFARDGETLEGVVGALLRDQGRTIAV
                     AESCTGGLVTDRLTDVPGSSAYLERGLTTYSNRAKMELLGVPEEVLQAHGAVSEETAR
                     LMAEGVRLRSGTDLGLATTGIAGPDGGSESKPVGTVYIALADGVETRCRHYRYRWERR
                     RIKMIASQAALLMVKRYLAG"
     gene            complement(5344..5778)
                     /locus_tag="PLT63_08010"
     CDS             complement(5344..5778)
                     /locus_tag="PLT63_08010"
                     /inference="COORDINATES: protein motif:HMM:NF016489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HOD98706.1"
                     /translation="MATGCGSGYFPFASGTAGTIAAIPLYLALSSLSGPLYLLTAVAF
                     TFLAIYAAREAEVIFGQQDSPRIVVDEWVGFLWTMLFIPPTLPHVVGGLVLFRFFDIV
                     KLFPANVFQDRLPGGYGVVMDDVAAGVYANIGLHLLIRGGII"
     gene            complement(5974..6549)
                     /locus_tag="PLT63_08015"
     CDS             complement(5974..6549)
                     /locus_tag="PLT63_08015"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOD98707.1"
                     /translation="MRKGQEEVEKKPEEKVEELLKINKLLEQENAEKKVAEEALKKRE
                     SELEATAKSLEELNAALKVLLKHREDDKNEVGVKVIANVKELVLPYVEKLKATPLSDT
                     QATLVDIVEMHLNEITSPFLMKLTSTYLGFTPKEIQVASLIRDGKKTKEIAKVMGVSK
                     SAIDLHRNHIRNKLGLNNKKVNLRSYLSALP"
     gene            6870..7736
                     /locus_tag="PLT63_08020"
     CDS             6870..7736
                     /locus_tag="PLT63_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98708.1"
                     /translation="MKKTLVAIVVVFVMSFAAGAAAENTTIFKIGEDVLIEEGSRVNH
                     VLTINGQITVTGTVEGNVIAMGDSVVLTRKALVKGNVVVLGGVLVKAKGADVQGTITE
                     INSSNISEVITHVLSDEWEGWSWVFAIFSLTIFFSILIIAILMVLLIPKPIQVIAASI
                     REETMKITLWGLLGLVLVVPLAILLTVSVIGIVLIPLEMILVVVAGLLGLIAVSQLVG
                     RKLYALFKRPGQHICRETFWGLVVLWSVGWIPYIGWMIKVLALMMGLGAVIYTRFGTH
                     APRRLSSSNINV"
     gene            complement(7848..>8336)
                     /locus_tag="PLT63_08025"
     CDS             complement(7848..>8336)
                     /locus_tag="PLT63_08025"
                     /inference="COORDINATES: protein motif:HMM:NF014867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HOD98709.1"
                     /translation="IAAELRVSVENVHATVLGGHGDTMVPLPRYTTVAGIPLPELLTP
                     ERIATLVERTRNGGAEIVSLLKTGSAFYAPASAAVEMAEAILRDRQKILPCAVHLQGE
                     YGIDGLFVGVPVKLGRGGVEEIIRLNLTDGELADLKKSAAAVGELVAAVKRINYPESR
                     IS"
BASE COUNT         1447 a         2643 c         2340 g         1706 t
ORIGIN      
        1 cccgccgatt tcttcagctc cgcctccacg gccttgaagt acttgatcgc ctcgcccccc
       61 gtcaccgcct cgatcctccg gacccccgcc gccaccgccg actccccgac gaccttcagg
      121 gcgccgatgt cgccggtgcg gttgacgtgg gtgccgccgc agagctccat gctgaagtcc
      181 cccatcttca ccacccggac gttgtcgctg tacttctcgt cgaagaccgc cgccgccccg
      241 gtcttcatcg cctcctcctt ggggaggacc cgggtgttca cctcggcgtt ccggcggatg
      301 tattcgttgg cgatgttctc gatggcgtcc agctcctccg cctcgatgcg gctgaagtgg
      361 gtgaagtcga agcggaaccg ctccggggtc accgacgagc ccgcctgctt gatatggtcg
      421 cccagaacct ttttcagggc ggcgttgagg acgtgggtgc ccgagtggtg ggcctccgtg
      481 gcccgccggg acgtctcgtc cacccggagc tgcaccgtgt cgcctacccg gatctttccc
      541 tccttcatct tgccgatatg ggtgatcagg gtgtccgtcg ggcactgggt gtcccagacc
      601 tcgaagagat agccgtcacc ggcgatcacc cccgtgtccc ccacctggcc gcccttttcc
      661 ccgtagaagg gcgtttcgtc cacgaagatc tcgccgtttt ccccggcgac aagttcctcg
      721 acctccgcgt ccttcttcag gatcgccgtg accacggccg cggcctcggt gacgccgtga
      781 tatccgatga atgccgtggt tacgccccgg acggagagct tcttgtagct gtccgccacc
      841 gcctgctcgc cgctgccctt ccaggattcc cgggccttct cccgctgggc ctccatggcc
      901 gcctcgaacc cttccatatc cagggtcaga ccgtccttgc ggacgatgtc cgccgtgagg
      961 tccacgggaa acccgaaggt gtcgtagagc ttgaagacca cgtcgccggg gatgacctgc
     1021 cggccctctt tcttgaggag ggcgacctcc tcgccgagaa tccggagccc cgtgtccagg
     1081 gtatcgatga accgctgctc ctcgttgacc acgaccttgc ggatatagga ttccttgtcg
     1141 atcagatccg gataggcctc ctgcatcacc gtcacgacca gggaaacgac ctcggggagg
     1201 aaggggcggt cgatccccag gagtttgccg tgccgggccg cccggcggag gattcgcctc
     1261 aggacatagc cccgcccctc gttgctgggc aggacgccgt cgccgataag gaaggtgaca
     1321 gcccgcccgt ggtcggcaat gacccgcatg gaaacgtcgg cctcggcatt ggcgccgtaa
     1381 gcccgcccgg caatcccggc gatcccggcg atgatcgggg tgaacaggtc cgtgtcatag
     1441 ttgctctgca ccccctggac gatggccgcc aggcgctcca gacccatgcc cgtgtcgatg
     1501 ttcggcttgg ggaggggctg gaagttgccg tcctggtccc ggtcgaactg ggtgaagacg
     1561 tggttccaga tctccaggtg gcggtcgcag tcgcactcca cggagcattc cgggcggccg
     1621 cagcccacgg acggcccctg gtcatagagg atctccgagc aggggccgca gggacccgtc
     1681 tcgcccatca tccagaagtt gctttccatc cccatgcgga cgatccgctc cgccgggacc
     1741 cccatctgct tgttccagat ctcgaaggcc tcgtcgtcat ccttatagat ggtgatccag
     1801 agcttgtctt ttgggagccc catgttcacc gtgaggaact cccagcccca ggcgatggac
     1861 tccttcttga agtagtcacc gaaggagaag ttgcccagca tctcgaagaa ggtgtgatgg
     1921 cgggcggtgt aaccgacgtt ctccaagtcg ttgtgtttcc ccccggcccg gacggacttc
     1981 tgggaactgg cggccctgct gtagccccgg tcttccaggc ccaggaagca gttcttgaac
     2041 tggaccatcc cggaattggt gaacaggagg gtcggatcgt ccttggggat cagactcgaa
     2101 ctcggcacga tggtgtgtcc gtggccctcg aagaatctca gaaactgctc tcttatttca
     2161 cttgccgtca tcgtcatgcc cgtcttcctc ctccgcgcgg ttaatcagct cgtaaatcag
     2221 tcccggggca aatccccggc ccagaagata ccggaaaagc cgttgcttgc cctgcggatc
     2281 ttccctgagc ggtctgcttt tcgtcttttt tttgattaag gatcttactg cctgttcttc
     2341 gctccactcc tgccggatgg cggtgatcgc ttcttccgcc agggatgcgg atattccctt
     2401 ttgcacaagc aaggagtgga gccgcctgtc tcccaggcct ttatcctccg ccaggcggcg
     2461 ggccaggccc gccgcaaaca gcttgtcgtt taaatacccc agtccgcgaa aataatcaat
     2521 cgttccggta acaacctctc ccgggaaacc cttttttgtc agtttccggg ccagctcctc
     2581 ctccccgtga tcccgccggg ccaaaagtcg ccagcagacc tgtttcgccc tggccagcnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnct gcaccttgcc gtcattctgc
     2761 cacggccgcg gccttcagac cgaatttttc ccgcaccccg gattcgacct cctggaggac
     2821 ttccggatgc tctttgagga aattgcgggt gttgtccctt ccctgaccca gacgctcccc
     2881 cttgtaggag taccaggcgc cactcttttc gatgatggcg ttttccgccg ccaggtcgag
     2941 gagatcgcct tcccgggaaa tcccttcgcc gaagatgatg tcgaactccg tctcccggaa
     3001 cggcggggcg accttgttct tcaccacctt gactcgggtt cgcatgccga tgacgtcctg
     3061 cccctgtttg agggagctca ccttccggat gtcgaggcgc atggaggcgt agaatttcag
     3121 ggcgttgcct cccgtggtgg tttcgggact gccgaagaag accccgatct tcatgcgcaa
     3181 ctgattgatg aagatcaccg tggtccgcga tttgctgata ctccccgtga gcttccggag
     3241 ggcctgggac atgaggcggg cctggaggcc catttgggca tcccccatct ccccttccag
     3301 ctccgcccgg ggcaccaggg ctgccacgga atcgaccacg aggacgtcga gggcgccgct
     3361 gcgcaccagg gtttcggcga tctccagggc ctgctcgccc gtatccggct gggcgatgag
     3421 cagctcgtcg gtattgaccc cgatcttccg ggcatagccc acatccaggg cgtgttccgc
     3481 atcgatgaag gccgccaggc cgttctgcct ctgggcctcg gcgacgatgt ggagggccag
     3541 cgttgtcttg ccgcccgatt caggaccata gatctcgatg atccgccccc ggggcacacc
     3601 gcccactccc agggcgatgt ccaggctgag ggttcccgtg ggaattaccg ggacatcgat
     3661 cttctccgat ccccccagct tcatgatggc ccctttgccg aactgccgct cgatctgact
     3721 gatggccaga tccacagcct tgttcctgtc catatctacc cccatcgcca tcctccttct
     3781 ctaattgttg aatattatcg ttaataaagc tgtctaagca atgaaacggc taaagtcaaa
     3841 aaatgggtcc cgccctcatc aaccccggag gggaaaggcg gccaaccggg tatagatcgc
     3901 cccccggggc gtcagttcgc ttttgaacag ggcgatctca ttcaccggga tccgcccggc
     3961 cgccacggcc gcctccgtct ccatcgcccn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnt cccccgccag ataccgtttc accatgagga gggccgcctg ggaggcgatc
     4141 atcttgatcc ggcgccgctc ccagcggtaa cggtaatggc ggcaccgggt ctccaccccg
     4201 tccgccaggg cgatatagac cgtccccacc ggcttggact ccgacccgcc atccggcccc
     4261 gcaatcccgg tggtcgccag ccccagatcc gtccccgacc ggagccgcac cccttccgcc
     4321 atgagccggg ccgtctcctc actcaccgcg ccatgggcct ggagcacctc ctccgggacc
     4381 cccaggagct ccatcttcgc ccggttgctg tatgtcgtaa gaccccgctc cagataagcg
     4441 gagcttcccg ggacatccgt caggcggtcg gtgacgaggc cgcccgtgca ggattccgcc
     4501 acggcgatgg tccgtccctg atcccggagc agcgccccta ccaccccctc cagggtctcc
     4561 ccgtcccggg cgaagatata acggtcgaga cgccggcgga cctcctcctc caccgccgcc
     4621 agccgcgccc gggccgccgc ctcgtcgtgt tcgtcccgga ccgacaggac gatgtgattc
     4681 tcgggaaaac ggggatagaa gccgatctcc acacccgcca gttccacccc ggccacggcc
     4741 tgatccaccg ccgcctcgga gaggccgaag gtcttcaggg tccgcacggc cgtatggcgg
     4801 gcatggccgg ggaaggcccg tttcaggagc ggaacgacgc ccgccggcac catccgccgc
     4861 acctccgccg gaacgccggg gatcacggcg atgaccttcc cctcccgccg cagggagaag
     4921 ccgggggcgg tgcccgtggg attggcgatg atctccgctc cctggggaaa gacggcctgc
     4981 ttggcgttat tcggggtcca tttcagtccg taacgggtaa agatccctcg gatgtgctgg
     5041 agggtctctt catcggtgat cagcggcagg ccgaagatat gggccagcgc ggccgtcgtg
     5101 atgtcgtcgg cggtgggtcc caggccaccg gtgacgatca ccgtctccgc ccggggaagg
     5161 atgaacgcca gggccgcagc gatcatctcc tcttcgtccc ccacggagag ggaggccacg
     5221 acctgccagc cctgaagatg gaactgccgg gcgaggaagg cggtattcgc atcctggata
     5281 cgcccgcttg tcagttcgtt gccgatggta attatgccga tatgcatgtg cgccgtacct
     5341 cccttaaata atcccgccgc ggatcaggag gtgcaggccg atattggcat agacccctgc
     5401 cgccacgtcg tccataacca ctccataccc gcccggcagc cggtcctgga agacattggc
     5461 gggaaagagc ttcacgatgt cgaaaaaacg aaacagcacc aaccctccca cgacatgggg
     5521 aagcgtcggg ggaatgaaga gcatcgtcca gaggaacccg acccattcat ccacaacgat
     5581 gcggggggaa tcctgctggc cgaagatcac ctccgcctcc cgggcggcat aaatggccag
     5641 aaaggtaaag gcgaccgccg tcagcaggta cagcggcccg gacagcgacg acagcgccag
     5701 atagaggggg atggcggcga tggttcccgc cgtccccgaa gcgaagggga aatagccgct
     5761 gccgcacccg gtggcgatga ctttgatcag ggatttattc agacctcacc tcacccaagt
     5821 ggtgggccag gctatcccct tttctcgcgg ccgtcaacag gaaatccctt gggaaattcc
     5881 ccaggccgac ggggcggaca tctcctgccg gcaggcactc ccggaggggt gcctgccgcg
     5941 gaaatccgtc tttatactcg ggggatgatg atgctaaggc agcgctgaca gataggaccg
     6001 caggttgacc ttcttgttgt ttaatcccag cttgttgcgg atgtgattcc ggtgcaggtc
     6061 gatggcgctt ttggaaacgc ccatgacttt ggcgatttcc ttggtcttct tcccgtcgcg
     6121 gatcaggctg gccacctgaa tctccttggg ggtgaagccc agataggtgg aggtcagttt
     6181 catgaggaac ggggaagtga tctcgttgag gtgcatttcg actatatcca cgagggtcgc
     6241 ctgggtatca ctcagcggcg tcgccttgag tttctccaca tagggcagca ccagttcctt
     6301 gacattggcg ataaccttga ccccgacttc attcttgtcg tcttcacggt gtttcaacag
     6361 gactttgagg gcggcattga gttcttccaa agatttggca gtcgcttcaa gctccgattc
     6421 ccgtttcttc agggcctcct cagccacttt cttttccgcg ttttcctgtt ccagcagctt
     6481 gttgatcttg agcaattcct ctactttctc ttccggcttc ttttccactt cttcctgtcc
     6541 tttacgcatt ttttactctc ctcttattat ttacaacaat gctgttaatg gtattgatcc
     6601 agtgacaaat acgattaaga gctgaattat atataatctt tctgataaca tacattttat
     6661 atcagctata atctggtggt actaatatcg aaacggagca agaaatgcaa gcgttttttg
     6721 cggtaattat caagacgggg gcaaaaagta ttttttatta taatatcgga ataatgccgt
     6781 cacttagtca ttgacagcgg gtggtctttt cgatactaac ggccatgatt aatgggaccg
     6841 caatgaagta aagataatat ggaaacaaca tgaagaaaac tctggtagcg attgtggtgg
     6901 tttttgtcat gagtttcgcg gccggcgcgg cggcggaaaa caccacgatc ttcaaaatcg
     6961 gtgaagacgt cctgattgaa gaaggatcac gggtgaacca tgtcctgacc atcaacggtc
     7021 agatcaccgt caccggtacg gtggagggta atgtcatcgc catgggcgat tcggtggtgt
     7081 tgaccagaaa ggccctggtg aagggaaatg tggtcgtgct ggggggcgtc ctcgtcaagg
     7141 ccaagggggc ggatgtccag gggaccataa cggagatcaa ctcctcgaac atctccgaag
     7201 tgatcaccca cgtcctgagc gacgagtggg aaggttggtc ctgggtcttc gcgatctttt
     7261 ccctgaccat ttttttcagc atcctgatca tcgccatcct catggtcctc ctgatcccca
     7321 aacccatcca ggtcattgcc gcgtccatcc gggaggaaac aatgaagatc accctctggg
     7381 gactcttggg gctggtcctc gtggtccccc tggcgatcct tctcaccgtc tccgtcatcg
     7441 gcatcgtcct catccccctg gagatgatcc tcgtggtcgt ggcgggactc ctgggcctta
     7501 tcgccgtctc ccagcttgtg ggccgcaagc tctacgccct tttcaaacgg cccggccagc
     7561 acatctgccg ggagacgttc tgggggcttg tcgtcctgtg gagcgttggc tggataccct
     7621 acatcggctg gatgatcaag gtcctggccc tcatgatggg tctgggcgcc gtgatctaca
     7681 cccgcttcgg aacccacgcc ccgcgccgcc tgtcttcgtc caacatcaac gtctaacagg
     7741 ttttttacga gaccatcgac tctgaccgtg tctcacaaga agtcgcaaat caccctcccc
     7801 cttgaaggga aaaataatgg ggaggaataa aatgactttc tatgcggtca gctaatacgg
     7861 ctctctggat aatttatgcg cttgacggcc gccacgagct cgcccacggc ggcggccgat
     7921 ttcttgaggt ccgccagttc cccgtcggtg aggttcaggc ggatgatctc ctccacgccc
     7981 cccctcccca gcttcacggg gaccccgacg aacaggccgt cgatcccgta ttccccctgg
     8041 agatggacgg cgcagggcag gatcttctgc cggtcccgga ggatggcctc cgccatctcc
     8101 accgccgccg aggccggggc gtagaaggcg ctgccggtct tgaggaggct cacgatctcc
     8161 gccccgccgt tccgggtccg ctccaccagc gtggcgatcc gctccggcgt caggagctcc
     8221 ggcaggggaa tgccggccac cgtggtgtag cgcggcaggg gaaccatggt gtcaccgtgg
     8281 ccgcccagga cggtggcgtg gacgttctcc acggaaaccc gcagctccgc ggcgat
//
LOCUS       DAOSMF010000081         8198 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_9760_length_8198_cov_17.945597, whole genome shotgun sequence.
ACCESSION   DAOSMF010000081 DAOSMF010000000
VERSION     DAOSMF010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 8198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8198
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9760_length_8198_cov_17.945597"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            198..425
                     /locus_tag="PLT63_08030"
     CDS             198..425
                     /locus_tag="PLT63_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98710.1"
                     /translation="MKFKTLMMGVCAALAVVSVEPMTGLLEVSVALVGAAVGAVIADP
                     IAEEAAEVKKQIQAVKEAVAQRRAAAAPVVA"
     gene            460..648
                     /locus_tag="PLT63_08035"
     CDS             460..648
                     /locus_tag="PLT63_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98711.1"
                     /translation="MQENNLFKDHTLQEVLDELDMIECFEVPGQKLQVGETTKHQMGI
                     FPKLGVKPPEPRYNNAGI"
     gene            complement(1047..2090)
                     /locus_tag="PLT63_08040"
     CDS             complement(1047..2090)
                     /locus_tag="PLT63_08040"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HOD98712.1"
                     /translation="MRFGRYVGLLLLGTVFLCCSCSAPKIKTTVLAPAQYHDAAKLKK
                     IAFLPFDGPGGNEFAMELEGALGSIQVGGSPFFTLVERQKIDQTIQEQRFSHSGLVAV
                     EDAVRLGKILGTRGIVTGTVTTSAVNDQRYREERRACIERERRVDKDGRVREGSCIRW
                     RNYYVGCTYREAIFSVSPKFIDVETSRIVYASTLSEKQISQACEDRNTPLASRQTLLA
                     AVKSRVINGIKKDIAPHYVIMEIQLMDDTDGMNKEAKELFKSGLEFAENDRLDRACEL
                     WDRALPLAGQSPALIYNVAFCLEIKGDLDQSLAMYRRADRLTTKPDDLISAAMKRLSE
                     KISSRDKLNDQVR"
     gene            complement(2265..2945)
                     /locus_tag="PLT63_08045"
     CDS             complement(2265..2945)
                     /locus_tag="PLT63_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98713.1"
                     /translation="MRKKTPRSAMIAPIVMMLLFLGGCTPVYLVQVNGFTDPDAAGRI
                     VPNVKIHVAENARAANPLLDREIRVKIEKILKSKGFIITSRETADLFCTFNYGVGTGL
                     TQIGSIPVTSPPVTSSVVVSQPDGTAQVSAIMTPGTTTYIPYARTAYDRWLNIVVLEA
                     ESFRAGEKDKILWYGDLVSSGTSRDLRTVIDYLLVAACEEFGTDTKRGLVKEIKADDE
                     RLKGFRRD"
     gene            complement(3010..5190)
                     /locus_tag="PLT63_08050"
     CDS             complement(3010..5190)
                     /locus_tag="PLT63_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98714.1"
                     /translation="MGEAVAESRKNDVRHHWRFFRAGGFDQVRLESGQDLTVLGQLDQ
                     KLWIALSCPVTGLEFDRKTLELIDTDNDGRIRAPEIIAAVSWAASLLKNPDDLLKGSA
                     ELPLAAIDDRSEEGSRLLSSAKHILATLGKDGAAAITPTDTADTEKIFSQTRFNGDGI
                     IPPEVAGDPELARIITTIMDCCGADMDRSGQPGIAQEKVDTFYTDLQAYAAWYDEAAV
                     GKGICPLGEDTEAAFGTLNAVRAKMDDYFTRCRLAAFDDRALNALNRQEEEYIAIAKG
                     NLNVAAEEFAGFPLAMIAAGRPLPLREGVNPAWETAVAALAATMVAPLLGARESLSEA
                     DWRQLQAAFASYEDWLTRKPATVVESLGIERVREILAGNGKAAIEELMVRDRAVEPEF
                     TAIADLDRLVRYHRDLITLLKNFVSFSDFYTRKDKALFQAGVLYLDSRSCDLCIRVDD
                     IAKHASLATMSQTYLAYCHCTRKNEEGYSTEMDIAAAFTDGDADCLMPGRNGVFYDRQ
                     GRDWDATIVKVIEHPISIRQAFWSPYKRIARMINEQIEKMAASRDKDVDDKARTGVAD
                     SAQKIEDGKAAAPAKPPFDVAKFAGIFAAIGLAVGAIGTALAAVITGFMKLIWWQMPL
                     AIAGIMLIISGPSMVIAWLKLRQRTLGPILDANGWAVNARAKINIPFGRSLTKVAALP
                     PGAERSLEDPFAEKKSPWPKLLFLLAIILALLYFFADKCPLLNR"
     gene            complement(5352..5537)
                     /locus_tag="PLT63_08055"
     CDS             complement(5352..5537)
                     /locus_tag="PLT63_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98715.1"
                     /translation="MSKLELTREEEKHLLEILERYYPSLRIEVVNTDDREFRRALKQR
                     EAFMKDLIGRLKVSLAT"
     gene            complement(5586..5843)
                     /locus_tag="PLT63_08060"
     CDS             complement(5586..5843)
                     /locus_tag="PLT63_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98716.1"
                     /translation="MYFMMICTWDPKDEKEVRTRRATWEWPEDVKVISEFYDLQGCRT
                     IYVVETDAKGLIAARAAWIDVLYFEIFPVYPIGETKELLMK"
     gene            6197..6979
                     /locus_tag="PLT63_08065"
     CDS             6197..6979
                     /locus_tag="PLT63_08065"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOD98717.1"
                     /translation="MNGTYSGAPAMMIIDSHMHCGIQNVDQPFGMIKGYLAQAGIDGA
                     CLYAPVEDIYDRYDYYFQDNALWAACRQRANRYLLDIQQEERDIHAYYFVWNDFRKDE
                     LAKGYQGVKWHRHDSEPEYHYDDPRCEAFLQEVYRLRLPIVLEESYGNTLALIKRIDG
                     RTQVIIPHLGGLNGGFYALFHSGVWNEENVYADTALAPRREMAAFLERYGSDRLIFGS
                     DFPFGIPYSELRKVRDLNLAEDEYAKVVCGNILKLINPRGLC"
     gene            complement(6957..7256)
                     /locus_tag="PLT63_08070"
     CDS             complement(6957..7256)
                     /locus_tag="PLT63_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98718.1"
                     /translation="MKKKLESLALTRISACFTISSLAGSDTGAAIVALAELEDMLDKF
                     YAEHKELVSPKEYELAKESPDAFIALIDRTHRHYLRLAEEGGGDSQKTVNKVPAD"
     gene            complement(7470..7781)
                     /locus_tag="PLT63_08075"
     CDS             complement(7470..7781)
                     /locus_tag="PLT63_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98719.1"
                     /translation="MELSDPTRKAFLTLFRKGVYYRATGVVLMEMGADLPVQYSLFYD
                     PVRADKVRSLYEAMDEIGSRFGKHTLRLGGSYLIEVAGKGKRGAATVREATQFFGETK
                     R"
     gene            complement(7844..>8198)
                     /locus_tag="PLT63_08080"
     CDS             complement(7844..>8198)
                     /locus_tag="PLT63_08080"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOD98720.1"
                     /translation="ADITGLRRPLRSSYEAIARRIKIEIQRDTGLTVSVGLSLTKVMA
                     KVGSKHRKPDGLTVIPGRAIASYLQDLQMEKIWSIGPATTDADYLFAQLLRNLESACL
                     KARRYKLAPRRLAFF"
BASE COUNT         1698 a         2242 c         2347 g         1911 t
ORIGIN      
        1 tctgataggg aagggtagat gaacgtcatt ggggaaatgg gattgtcgat atggatgccg
       61 gcgagatttg gtgtcgagtt tagaaaatat atgccgacat agagctccgt acagatcgaa
      121 aggacctaaa ggatttgata ttcagaaatt tgatatagaa attgtggtgt tatcaaaatc
      181 gaaaggagga cgtggatatg aagttcaaga cattgatgat gggggtatgc gctgccctgg
      241 cggttgtttc cgttgaacca atgaccggat tgctggaggt gtcagtggcc ctggttggtg
      301 ctgcggtggg agcagtaatt gccgatccta ttgcagaaga agcagcggag gtcaaaaaac
      361 agatccaggc agtgaaggaa gctgtggcac aaagaagagc ggcagcggca cccgttgtgg
      421 cttgagtgtt caattcattt gtcctacatt acaaggaaaa tgcaggagaa caaccttttt
      481 aaggaccata ccttacagga agttctggat gaactcgata tgattgaatg ctttgaagtc
      541 cctggccaga aactgcaggt tggtgaaaca acgaaacacc agatgggaat ttttcccaaa
      601 ctgggggtca agcctccaga gcctcgttac aataacgcgg gaatttagga tacatagtcg
      661 catcgcctat ggctgcggcg ccgtgaataa gtgaaatcga tgtcgcccat gcggatgacg
      721 gtcttgttgc ccccatccat acatcctgag gcacaggaag caatgcacag ccggcatgaa
      781 tcacagtaat tgtcttccag cggcaatggc gcggtgggaa tcaatgctgc cgatgtaacc
      841 agcgacccca agatcaccgc ctcaccatac tcactgataa tgacatttcc ggaaagaccg
      901 aaatggccga tgccggacct gacagccaaa tatctatgag aaacatgcgg cccggggtat
      961 gtccctcgcc tccggcaaag tttattccgt agaatggccg gccatactgt tcatgtcttt
     1021 atatggacct gatctcttct gccgggttac ctcacctgat cgttcaattt gtctctgctg
     1081 ctgatttttt ccgagagccg tttcatggcg gcggagatca ggtcgtcggg tttggtcgta
     1141 agccgatccg cccggcggta catggcgagg gactgatcca ggtccccctt tatttccaaa
     1201 cagaaggcga cgttataaat cagcgccggc gattgaccgg ccagaggcaa cgcccggtcc
     1261 cagagctcgc aggcgcgatc caggcggtcg ttttccgcaa attccaggcc ggatttgaac
     1321 aattcttttg cctctttgtt catgccatcc gtatcatcca tcagttggat ctccatgatg
     1381 acgtaatgag gcgcgatatc cttcttaatt ccgtttatga cacggctttt gaccgcggca
     1441 aggagcgtct gccggctggc cagaggcgta ttcctgtcct cgcatgcctg ggaaatctgt
     1501 ttctccgaca gggtggaggc ataaacgatc cgactggttt ccacgtcgat gaacttcgga
     1561 ctgaccgaga aaattgcctc gcggtaggtg cagccgacat aatagttacg ccagcggatg
     1621 caggaacctt cccgaactct cccgtccttg tccactcttc tttccctttc aatgcaggcc
     1681 cgtcgttctt cccggtatcg ctggtcgtta accgccgaag tcgtaacggt gccggtgaca
     1741 attcctcggg tgcccaggat ctttcccaga cggactgcgt cttccaccgc tacgagtccg
     1801 ctgtgggaaa acctctgctc ctggatggtt tggtcgattt tctgtctttc caccagcgtg
     1861 aagaaagggc ttccccccac ctggatgcta cccagcgccc cttccagctc catggcgaat
     1921 tcatttcccc cgggaccgtc gaaaggcaga aaggcgatct ttttcagttt cgccgcgtcg
     1981 tgatattgag ccggcgccag taccgtcgtc ttgattttag gggccgaaca ggaacaacaa
     2041 agaaaaacgg tgcccaaaag aagaagaccc acatatcgtc cgaacctcat gatttcctcc
     2101 tgtttgcatc tcgaacccat agcgatggac ttgatacttt cataaacacc ggtaagacct
     2161 acaagcgcga cgaaccggca tttcatcccg ccggtattgg ttataatgtc cagcagatct
     2221 ttacgctcgg ggaggacatc cccggcgggc cgctggcgcg ggcatcaatc gcgccggaag
     2281 cccttgagcc tttcatcatc cgcttttatt tctttgacca gaccccgttt cgtgtccgtc
     2341 ccgaattcct cgcaggccgc cacgagcagg taatcgatta ccgtccgcag gtccctgctc
     2401 gttccggaac tgacgaggtc accgtaccag agaattttgt ctttttcccc tgcccggaaa
     2461 gactcggcct ccaggacgac gatattcagc caccgatcgt aagccgtcct ggcataaggt
     2521 atataggtgg ttgtcccggg tgtcatgatc gcgctcacct gggcggtgcc gtccggttgg
     2581 gaaacaacca ccgaggatgt cacgggggga gacgtcacgg gaatcgatcc gatctgtgta
     2641 agcccggtcc ccactccata attgaaggtg cagaacaggt cggccgtttc ccggctggtg
     2701 ataataaacc ccttgctttt caatatcttt tcaatcttta ccctgatctc tcgatccagc
     2761 aggggattcg ccgcccgggc attttcggcg acatgtatct tgacgttcgg cacgatgcgg
     2821 ccggcggcgt cggggtcggt aaagccgttg acctggacca agtaaacggg ggtgcagccc
     2881 cccaaaaaca gcagcatcat cacgatgggg gcgatcattg cagaccgggg tgtttttttt
     2941 ctcacggtat ccgtatttcc tttatcggcg cctgagcagc ggcggaagat ctttccgtcc
     3001 cattcagcct taccggttca gcagagggca tttatcggcg aaaaaataga gcagggccag
     3061 gatgatggca agcaagaaga ggagctttgg ccagggactc tttttttccg cgaagggatc
     3121 ctcgagggat cgttccgccc cggggggtaa ggcggccacc ttggtcaggg agcgaccgaa
     3181 ggggatattg atcttggccc gggcgttgac cgcccagccg ttggcgtcga ggatgggtcc
     3241 gagggtgcgc tgccggagtt tcagccaggc aatgaccatg gaaggtccgg agatgatcag
     3301 catgatgccg gcgatggcca ggggcatctg ccaccagatc agtttcatga atccggtgat
     3361 caccgcggcc agggccgtgc cgatggcgcc gacggccagg ccgatggcgg cgaagatacc
     3421 ggcgaacttg gcgacatcga aaggaggttt ggccggtgcc gccgccttcc catcctcgat
     3481 tttttgggcg ctgtcggcga cgccggtccg ggccttatcg tctacgtcct tgtctcggga
     3541 ggcggccatc ttctctatct gctcgttgat catccgggcg attcgtttat aggggctcca
     3601 gaaggcttgg cgaatactga tgggatgctc gatgaccttc acgatggtgg catcccagtc
     3661 ccggccctgg cggtcgtaga aaactccgtt ccgtcccggc atcaggcagt cggcgtcccc
     3721 gtccgtaaag gcggcggcga tgtccatctc cgtgctgtag ccttcttcgt tcttccgcgt
     3781 gcagtggcaa taggccagat aagtctggct catggtggcc agggaggcgt gtttggcgat
     3841 atcgtcaaca cggatacaga gatcgcaact gcggctgtcc agatagagca caccggcctg
     3901 aaacagagcc ttgtctttgc gggtataaaa atcagaaaag gagacgaagt ttttcaacag
     3961 ggtgatcaga tcgcggtggt agcggacgag acggtccaag tcggcgatgg cggtgaattc
     4021 cggctccact gccctgtccc tgaccatcag ttcctcgatg gcggccttgc cgttgccggc
     4081 gaggatctcc cggacccgtt ctatcccgag gctttccacc actgttgcgg gtttgcgggt
     4141 caaccagtct tcgtaagagg caaaggcagc ttgcagttga cgccaatccg cctcggagag
     4201 gctctctctt gcccccaaga gcggcgccac cattgtggcg gcaagagcgg cgaccgccgt
     4261 ctcccaggcg gggttgacgc cttcccggag gggcaggggc ctgccggcgg cgatcatggc
     4321 taaaggaaag cccgcgaact cctccgcagc gacgttgagg ttgcccttgg cgatggcgat
     4381 gtattcctct tcctggcgat tgagagcgtt cagtgcccgg tcgtcgaagg cagcgagacg
     4441 acacctggtg aaatagtcgt ccatcttggc gcgcaccgcg ttcagggtac cgaaggccgc
     4501 ctcggtatcc tctcccaggg ggcaaatgcc ttttcccact gccgcctcat cataccaagc
     4561 ggcgtatgcc tgcaggtcgg tgtagaacgt atcaactttt tcctgggcga tgcccggttg
     4621 gccgctccgg tccatgtcgg ccccgcagca atccataatg gtggtgatga tccgtgccag
     4681 ttccggatcg ccggcgactt cgggggggat gatgccgtcg ccgttgaagc gggtctggga
     4741 gaagatcttt tccgtatccg cggtgtcggt gggggtgatg gcggcggcgc cgtccttccc
     4801 gagggtggcg aggatatgtt tcgccgagct caagagtctg cttccctctt ccgaccggtc
     4861 atcaatggcc gcgagaggta gctccgccga tcccttgagc agatcgtcgg gatttttcaa
     4921 cagtgatgcc gcccaggata cggcggcgat gatttccggg gcgcgaatgc gaccatcatt
     4981 atcggtatcg atcagctcca gggtcttccg gtcgaactcc agaccagtga cgggacagct
     5041 cagggcaatc cagagtttct gatccagttg tcccaggacg gtcaaatcct gtccagattc
     5101 cagccgcacc tgatcgaatc ctccggctcg gaaaaatctc cagtgatgtc ggacgtcgtt
     5161 ctttctgctt tccgcgactg cttctcccat gatgagttcc cctttcagag tagtggcggg
     5221 tattataaaa cgcaattcaa ttataggcaa tttatatctt aggggactcc cttccccggg
     5281 acggggaagg gagtgactgg cggccggtgt ggtgggttta gctgtcttta cggagtccga
     5341 ggtcttgatg cctatgtggc cagggagact ttcaggcggc cgatcaggtc tttcatgaag
     5401 gcctcccgct gtttcagcgc ccgtcggaat tccctatcgt cggtattgac cacttcgatc
     5461 cgcaaagacg gatagtatct ttccaggatc tccaggagat gtttttcttc ttcccgggtg
     5521 agttcaagtt tggacatggt atccctcctc ctttctcaca taactgatga agaccacgcc
     5581 acaacttatt tcatcagcag ctccttcgtt tcgccaattg gatagacggg gaaaatctcg
     5641 aaatacaata cgtcgatcca ggcagctcga gcggcaatga ggcctttggc gtccgtctcg
     5701 accacataga tggtacggca tccctgcagg tcgtaaaact ctgatatgac ttttacatcc
     5761 tccggccatt cccatgtcgc ccttctggtc ctgacttctt tttcgtcttt tgggtcccag
     5821 gtgcagatca tcatgaaata cataattctc acctccttgc ggttcttggg gaattcatcg
     5881 ctgacgtccg cagctctttt ccatggacat taagcttcgc ttttccgcct ccgggagatc
     5941 aatcgctctc ccggaggcgg gttatgggca ctttgcaggg caggttgatc atttcggcct
     6001 cctttttccg gcgccggaag tggcgcgaat gaaaaaagta tgctatgttg atgaaggttt
     6061 aaaggttatt tgttctggtt gacgatctac ggctggaaat aacgcgtcgg ttgaaataca
     6121 agctgctctt tctgatcata atataaaaca gagaaacagt gatgtcaact ggaccggcgg
     6181 tgcacgtcgg gagaaaatga atggaacata ttctggggca ccggcaatga tgattatcga
     6241 ttcccacatg cattgtggca tccagaacgt ggatcagcca tttggtatga tcaaggggta
     6301 tctcgcccag gccggcatcg acggggcctg cctgtatgcc ccggtggagg atatctacga
     6361 ccgctacgat tactatttcc aagacaacgc cctctgggcg gcctgccggc agcgggcgaa
     6421 ccggtatctc ctagacatcc agcaggagga acgggatatc catgcctatt atttcgtctg
     6481 gaacgatttc cgcaaggatg agctggcgaa ggggtaccag ggcgtcaagt ggcatcgcca
     6541 cgattccgaa ccggagtacc actacgacga tccccgttgc gaggcctttc tacaggaggt
     6601 ttaccggctg cggctaccca ttgtcctcga ggagtcctac gggaacaccc tggcgctgat
     6661 caaaaggata gacggccgga cccaggtgat cattccccat cttggggggt tgaacggggg
     6721 gttctacgcc ctctttcatt ccggggtctg gaacgaggaa aatgtttacg ccgacaccgc
     6781 tctggccccc cgccgggaga tggccgcgtt tctggagagg tacggtagcg acaggctcat
     6841 cttcggcagc gattttccct tcggtattcc ctacagcgaa ctgcggaagg tgcgggacct
     6901 gaacctggcg gaagacgagt atgccaaggt ggtctgcggg aacatcttga aactgatcaa
     6961 tccgcgggga ctttgttgac cgttttttgg gaatccccgc cgccttcctc cgccaggcgg
     7021 agataatgac ggtgtgtccg gtcgatgagg gcgataaagg cgtcggggct ctcctttgcc
     7081 agttcgtatt ctttcggaga gacaagttcc ttgtgctccg cgtagaactt gtcgagcata
     7141 tcctccagct ccgccagggc cacgatcgcg gcgccggtgt cgctgcccgc cagggaactg
     7201 atggtgaaac aggcgctgat cctggtcaag gcaagtgatt ccagcttttt tttcatgagt
     7261 catcctcccg tcgatggact gcgacttgtt gtcaagccgc gggaatctaa atgatttgtt
     7321 ccacaaagat caagagattt ttatcaatcc cgggtggctg ccgcctttct tacgggaagg
     7381 cctggagatt tatcacgcaa tatccgtaag atggcggctg acaacgggga agatcaaacc
     7441 ttcgcgcgca ggatcgagag tcccaggtgc taccgcttcg tttctccgaa gaattgcgta
     7501 gcttccctta ccgtcgccgc cccccttttc ccctttccgg ccacctcgat gaggtacgag
     7561 ccgccgagac gcagggtatg cttgccgaac cggctgccga tttcatccat ggcttcatag
     7621 aggctgcgca ctttgtccgc ccgcacgggg tcgtagaaca gggagtactg aacggggaga
     7681 tcggcgccca tctccatgag caccaccccg gttgcccggt aatatacccc cttccggaag
     7741 agggttagga aggccttccg ggtcggatca gataactcca ttgtgcatgc ggaaggccgg
     7801 ttgagaagca cttcacagcc gtggtataaa aagttattgg tcttcagaaa aacgctaggc
     7861 gccgcggggc cagtttgtaa cgcctggcct tcagacaggc cgattccagg ttgcggagca
     7921 gttgggcgaa gagataatca gcatccgtgg tggcggggcc gatgctccag atcttctcca
     7981 tctgcagatc ctgaaggtag gaggcgatgg cccgtccggg gatgacggtc agcccgtccg
     8041 gcttccggtg tttcgatccc accttggcca tgaccttcgt gaggctcagt cccaccgaga
     8101 cggtgagtcc ggtatccctt tgaatttcaa tctttattct tcgggcaatg gcctcgtagg
     8161 aggatctcag cgggcgccgt aaaccggtga tgtccgca
//
LOCUS       DAOSMF010000082         8158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_9827_length_8158_cov_7.956806, whole genome shotgun sequence.
ACCESSION   DAOSMF010000082 DAOSMF010000000
VERSION     DAOSMF010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 8158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8158
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9827_length_8158_cov_7.956806"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="PLT63_08085"
     CDS             <1..210
                     /locus_tag="PLT63_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98721.1"
                     /translation="QVLPAVMTPASALSFLVVLMLFIPCAATVVVMKQELENWRWFGA
                     SLALMLGLSFVLGFAAYRLALVIGL"
     gene            336..1475
                     /locus_tag="PLT63_08090"
     CDS             336..1475
                     /locus_tag="PLT63_08090"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOD98722.1"
                     /translation="MNFQLTEEQELIRRNAREFAERHVAPVAAAVDEKAFHPRELFAE
                     LGAGGWLGIPLPERYGGSGADLLTHAVVVEEISRACSATGFTLSFHAGIIGMSLHIFG
                     TEAQKERYLVPLAQGRHMGAFSLTEPGAGTDVMAVATTAVRDGGYYVLNGTKTFVSNG
                     PFADTYIFFCWTDRAAGKKGMSAFIVSKGAQGLQPGEPFHKMGLRGSQTSEVVLRDCR
                     VPEENMLGAPGQGLPIAMQGFEHGRVGIAAQATGILQAALDESLRYAKERVQFGVPIA
                     RHQAIAWMIADMDADLAAARLLTYQAAWLKDRQLPFGKAASIAKLFATEAAMRHTVKA
                     VQIQGGYGYIKGAKVERLMRDAKIAEIYEGTSEAQRLVISGSLLR"
     gene            1600..3150
                     /locus_tag="PLT63_08095"
     CDS             1600..3150
                     /locus_tag="PLT63_08095"
                     /inference="COORDINATES: protein motif:HMM:NF005760.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HOD98723.1"
                     /translation="MNGAEALLRTAGAGGIEVCFANAGTTEMPIVMALDAVPGIRPVL
                     GLFEGVCTGAADGYGRLKGSPAMTLLHLGPGLGNGIANLHNARRAGTPIFNVIGDHAS
                     WHLAADAPLTMDIAALAGTVSGWQRQNAAAAELPQDTALAIAAARSGMIATLVVPVDH
                     QGAACPEAPAGIPPFAFAPPDHQAVAEAACFLRTATRPLLILGGRALSAPGLTAAARI
                     RAAVGCDLLVETFPAVMARGAGLPAPAKIPYFPDHAVAVLKPYDQVLLAGARPPVAFF
                     GYEGLPGRLLGEEQRRLRLDGEGQDAAAALTALADFLAAPADGESLRDVLADYRRPEL
                     PAGKLTAEKAGLVLAALQPEGAVIVEEALTSGFSYFTPAAQVAPHTYVTITGGAIGQG
                     LPCATGAALACPDRRVIAFQADGSAMYTLQALWTQARESLNVTTLICANRGYQILQVE
                     MLRAGYLSPGPGALYLTELGKPCLDWVQISRGMGVPAVAVATAEDLARELSAALASPG
                     PRLIEMVL"
     gene            complement(3316..3666)
                     /locus_tag="PLT63_08100"
     CDS             complement(3316..3666)
                     /locus_tag="PLT63_08100"
                     /inference="COORDINATES: protein motif:HMM:NF016847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOD98724.1"
                     /translation="MIHRYWESLKIFLREAVPEKEPIPGAVIAIQTFGDFLGFNPHCH
                     ILVTDGCFCGSRGMFRMAPPRELKKLEAIFRHKVFRMVLNKGKTSEGMVKMLSTWRLS
                     GFNVFCRNRKRRDA"
     gene            3702..3926
                     /locus_tag="PLT63_08105"
     CDS             3702..3926
                     /locus_tag="PLT63_08105"
                     /inference="COORDINATES: protein motif:HMM:NF024934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOD98725.1"
                     /translation="MKNGRHKCPTAYLLYLSLERGGKDIIKLNISKPQLANLLGTGPE
                     PLPRAFGNMKSRKLVVEEGADIRLIKRGLL"
     gene            complement(4007..4135)
                     /locus_tag="PLT63_08110"
     CDS             complement(4007..4135)
                     /locus_tag="PLT63_08110"
                     /inference="COORDINATES: protein motif:HMM:NF014398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HOD98726.1"
                     /translation="MVTAENSMGFHNPDQALNTLGRSIDTAHQSIAAANRAAGASF"
     gene            4373..4807
                     /locus_tag="PLT63_08115"
     CDS             4373..4807
                     /locus_tag="PLT63_08115"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOD98727.1"
                     /translation="MKSEDRMIYLLSTARQALIDHTNKSLLKAGIKTTIAQAGVLFLL
                     IQRDMRNMSEFGQVFGIDKSAVTYLIDKLEKNGLVERNVQEGNRRAFLIHITPTGIKE
                     AKKAGEIVSGINQEITTSFDSREIEAYKKVLNGIIEKFRPPK"
     gene            4974..5324
                     /gene="hypA"
                     /locus_tag="PLT63_08120"
     CDS             4974..5324
                     /gene="hypA"
                     /locus_tag="PLT63_08120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HOD98728.1"
                     /translation="MHELPVTESILRIVLRHAEEAKAGKVIGISLRIGEMTDVIDEWL
                     KRYFDYLSKGTLAEGAKLRVERMPVIFRCEVCGETFPVKIREIKDVVCPACGGEKAIF
                     VSGREFFIKEIEVI"
     gene            5324..6022
                     /gene="hypB"
                     /locus_tag="PLT63_08125"
     CDS             5324..6022
                     /gene="hypB"
                     /locus_tag="PLT63_08125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HOD98729.1"
                     /translation="MSEVRLIEIKEEILVDNDKVAEKIRKQLWARQVFMLNLMSSPGA
                     GKTSLIIATLRSLNESMRAGVIEGDIDSLVDAEKVAQEGIPAVQLRTGGACHLDAVMV
                     ELALREMDLSALDLIIIENVGNLVCPAEFDTGAMRKVMILSVPEGDDKPLKYPLMFTV
                     CDVLVVNKTDYLKLSDFDMKTLKERVLKLNPQIKIFEVSCKTGEGMAAWTQWLRAEIE
                     TFKKMTEEEDHGRV"
     gene            6009..7091
                     /locus_tag="PLT63_08130"
     CDS             6009..7091
                     /locus_tag="PLT63_08130"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOD98730.1"
                     /translation="MDVYEKLREILDAHPTGAPPSPVFDKILRILFTPPEAELLTHMN
                     FSPKPVETIAAAAGAPVAEADRMLGALADKVLVFGREKNGKRSYGLLPAIPGLFEYPF
                     MRGEMTPELVHLGKLWEDYHRDGLGESFAGSPTPVTRVVPVEKSVAAPLQVHPYEEVK
                     KLIDSVDFISLGQCACRVTLKRCDKPTETCLFFDAPARFLVEKKYARQITRDEAYAVL
                     ERAEAEGLVHTSTNTADRVGFICNCCRCCCIILRGLTELNLPHAFAPSGFQAEVQADV
                     CIGCGLCADDRCPVGAIGMKDDLAVVETVKCIGCGLCVTACPVEAISLVRRTAAPEIP
                     ATNQEMLGKLLTEKGKIERFTKLMKS"
     gene            7102..7812
                     /locus_tag="PLT63_08135"
     CDS             7102..7812
                     /locus_tag="PLT63_08135"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOD98731.1"
                     /translation="MLSPELAALTVTAASLGFFHTLLGPDHYLPFVVMARARRWSQAR
                     TAWITLFCGLGHIGSSVVLGLAGITLGMAVTSLEGIEALRGDLAAWALIACGLVYFAW
                     GLKKAYRYRPHEHSHFHENGTAHTHRHIHGTDHAHVHGDNSRITPWVLFIIFVLGPCE
                     PLLPVLMYPAAKESLAGMLWVTGVFGTVTILTMMGIVLFSLRGISLIPLKGIERYTHA
                     LAGGTICLCGIAIQYLGV"
     gene            complement(7826..8014)
                     /locus_tag="PLT63_08140"
     CDS             complement(7826..8014)
                     /locus_tag="PLT63_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98732.1"
                     /translation="MAGRHALLIGDVLRKPARDRNVSPERKGEQIVRYNGHYSNVSRG
                     KRQTTGNDDAVPYLNFPI"
     gene            complement(8038..8158)
                     /locus_tag="PLT63_08145"
                     /pseudo
     CDS             complement(8038..8158)
                     /locus_tag="PLT63_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008328818.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
BASE COUNT         1670 a         2415 c         2459 g         1614 t
ORIGIN      
        1 caggtactgc ccgcggtgat gaccccggca tcggccctgt cctttctcgt cgtgctcatg
       61 ttgttcatcc cctgcgcggc cacggtggtg gtaatgaagc aggagctgga aaactggcgt
      121 tggttcgggg cctcactcgc cctgatgctg ggactgtcct ttgtcctggg cttcgccgcc
      181 taccggctgg ccctggttat cggcctgtga aatagatgaa taaattgggc gggtctcttt
      241 tttataacaa gcggtcccgc ctgccgtggt tggatctgaa ttaacagaca aagtgcggcg
      301 gcgtgcccaa gtatgtgaca aggagggtat gacagatgaa ttttcaactg acggaagaac
      361 aggaactcat tcgccggaat gcccgggagt ttgcggaaag gcacgtagcg cccgtggcgg
      421 cggcggttga cgaaaaggcc tttcatccca gggagctatt cgccgaactc ggcgccggcg
      481 gctggctggg gattcccctt ccggagcgat acgggggcag cggggccgat ctcctgaccc
      541 atgccgtcgt ggtggaggag atctcccggg cctgctccgc cacggggttc accctgtcct
      601 ttcatgccgg catcatcggc atgtcgctgc atatcttcgg cacggaggca cagaaagagc
      661 gctacctcgt tcccctggcc caggggcggc acatgggcgc cttttccctg acggaaccgg
      721 gggccggcac cgacgtcatg gccgtggcga ccaccgccgt ccgggacggc ggctactatg
      781 tcctcaacgg gaccaagacc ttcgtctcca acgggccctt tgccgatacc tacatcttct
      841 tctgctggac ggaccgggcg gcgggtaaaa aaggcatgag cgccttcatc gtttccaaag
      901 gcgcccaggg cctccagccc ggcgagcctt tccacaagat gggcctccgg ggatcccaga
      961 cctcggaggt ggtcctgcgg gactgccgcg ttccggagga aaatatgctg ggggcgccgg
     1021 ggcagggcct ccccatcgcc atgcagggct tcgagcacgg ccgggtgggc atcgccgccc
     1081 aggcgacggg gatccttcag gcggcccttg acgaatcgct ccgttatgcc aaagagcggg
     1141 tccaattcgg ggtccccatc gcccgccacc aggccattgc ctggatgatc gccgacatgg
     1201 acgccgatct ggccgcggcc cgcctgctta cctatcaggc cgcctggttg aaggaccgtc
     1261 aactgccctt cgggaaggcg gcgagcatcg ccaaactctt cgccacggaa gcggccatga
     1321 gacataccgt caaggccgtc cagatccagg gcggctacgg atacatcaag ggggcgaagg
     1381 tcgagcgact catgcgggac gccaagatcg ccgagattta cgagggcact tcggaggccc
     1441 agcggctggt gatctccggg agcctcctac gctgaaaaaa cgtgatattt caatgtgatg
     1501 agcaacgtct ccatgccggt gaaaccatga ttccttttgg ggagatcaga aaagaaatgg
     1561 tacgggcgtt gttatctcgc ggaaggaggg cgtgaaatca tgaacggagc ggaggcgctg
     1621 ctcaggacgg ccggggcggg aggcatcgag gtctgcttcg ccaatgcagg gaccacggag
     1681 atgcccatcg tcatggccct ggatgcggtt cccggcatcc ggccggtgct ggggttgttc
     1741 gaaggagtct gcaccggtgc cgccgacggt tatggtcgcc tgaagggttc gccggccatg
     1801 acgctcctcc acctgggacc ggggctgggc aacggtattg ccaatctcca caacgcccgg
     1861 cgggccggaa cgcccatttt caatgtcatc ggcgatcacg cctcctggca tttggccgcc
     1921 gacgccccgc tgaccatgga catcgccgcc ctggccggga cggtttccgg ctggcagcgg
     1981 caaaacgccg ccgcggccga actgccccag gacaccgccc tggccatcgc cgccgcccgt
     2041 agcgggatga tcgccaccct cgtcgtcccg gtggatcatc agggggcggc ctgcccggaa
     2101 gcgccggcgg ggatcccgcc ttttgccttt gctcctccgg accaccaggc agtggcagag
     2161 gccgcctgct tcctgcgcac cgccacccgt cccctcctca tcctgggcgg ccgggccttg
     2221 agcgcccccg gcctgacggc cgccgcccga atccgggccg ccgtcggctg cgacctgctg
     2281 gtcgaaacct ttcccgccgt catggcgcgg ggcgcggggc ttcccgcccc ggccaagatc
     2341 ccctatttcc ccgatcatgc cgtcgccgtc cttaagcctt acgatcaggt cctccttgcc
     2401 ggcgcccggc cgcccgtggc cttcttcggc tacgaaggtc tgcccggccg cctcctggga
     2461 gaggagcagc ggcggttgcg cctcgacggg gaagggcagg atgcggcggc ggccctgacg
     2521 gccctggccg actttcttgc tgcccccgcc gatggggaga gcctccggga tgtcctggcg
     2581 gactaccggc gccccgaact accggcgggg aaattgaccg ccgagaaggc cggtctggtc
     2641 ctggccgccc tccagccgga aggagctgtg attgtcgagg aggccctgac ctcgggattt
     2701 tcctatttta ccccggcggc ccaggtggcg ccccatacct atgttaccat taccggcggg
     2761 gccatcgggc aggggttgcc ctgcgccacc ggggccgccc tggcctgtcc cgaccggcgg
     2821 gtgatcgctt tccaggccga cggcagcgcc atgtacaccc tccaggccct ctggacccag
     2881 gcccgggaat ccctgaatgt caccaccctg atctgcgcca accggggcta ccagatcctc
     2941 caggtcgaga tgctccgggc gggttatttg tcgccgggcc ccggggcgct gtacctgacc
     3001 gaactgggga aaccctgcct ggactgggtg cagatcagcc ggggaatggg cgtaccggcc
     3061 gtcgcggtgg caacggcgga ggatcttgcc cgggaactgt ccgccgccct ggcctccccc
     3121 ggcccccgcc tcatcgagat ggtcctgtag gacatgacca cgtataggct tgcaccgaaa
     3181 atcaggcgcg gggacattgt atatggatcg gcggcatgcc ttttgaacaa tcggtatgtc
     3241 atttaggcgc atcgctcgtc aggcaggcaa tccccgcctc cgcggggaca agtttgaggc
     3301 gcttttcggg caaacctatg cgtcacgcct cttgcggttt ctgcagaaga cgttgaatcc
     3361 cgaaagtcgc catgtggaga gcattttcac catcccttca cttgttttac ccttattcag
     3421 caccatccgg aacaccttgt gccggaagat ggcctccagc ttttttagct cccggggcgg
     3481 ggccatccgg aacatgcctc tgctgccgca gaaacatccg tcggtgacga ggatgtggca
     3541 gtgcggattg aagccgagga agtctccgaa agtctgtatg gcaatgacgg cgccgggaat
     3601 gggttccttc tcgggcaccg cttcccggag aaatatcttc agcgattccc aataccgatg
     3661 gatcacctgt tcgatatagg gctgccagaa accgtattgc cgtgaaaaat ggtcgtcata
     3721 aatgtccgac cgcctatctg ctctacctgt cactggagcg gggagggaaa gacatcatca
     3781 agctgaacat ttcaaagccg cagcttgcca atcttctggg cacggggccg gagcccttac
     3841 cccgcgcctt tgggaacatg aaaagccgca agcttgtcgt ggaagaagga gccgatatca
     3901 ggctgatcaa acgcggcctg ttgtaagagc ttgccgagag cggcaaaggc gcccgatgag
     3961 aaaatctcgc ggttacatcc cggcagacaa tgcaaattcg agtattctaa aaggaagctc
     4021 ccgccgcccg attggcggcg gcaatggact gatgggccgt atcgatggag cggccaaggg
     4081 tgttgagcgc ttggtcggga ttatggaatc ccatgctgtt ttcggcggtt acaatgtctc
     4141 aaattggccg acttgcaggt tatacaggcg tcggggctgg ccggcgcgac cctttttgtt
     4201 ttcttcagat cctggatggc atagggatgg ccgcgttctt ccgtatagtc cgtgctgaat
     4261 gccatacact tgaaacggag gaatgcctgg aagacgataa atcaaaaaac ttgacatatc
     4321 ctttattgtt gaatattaaa ctgttgagac ttaaacacaa cgagaggaaa ccatgaaatc
     4381 cgaagatcgc atgatctatc ttttatctac agccaggcag gcattgatag accatacaaa
     4441 caaatcactt ctaaaggccg gaattaagac gaccatcgcc caagccggag ttctatttct
     4501 tcttattcag cgggacatgc ggaatatgtc cgaattcggc caagtctttg gaatcgacaa
     4561 atccgccgtg acttacctga tcgacaaact ggagaaaaac ggcttagtag agcgcaatgt
     4621 tcaggaggga aatcgccggg cctttttaat tcacataacc ccgacgggga taaaagaggc
     4681 aaaaaaagcg ggggaaatcg tcagcggcat caatcaagaa ataacaacga gtttcgattc
     4741 ccgggaaata gaagcctata aaaaagtttt gaacggcata attgagaaat tcagaccccc
     4801 taaatgagag agaaatgtaa acaagcctga cgcagcttat gggggacgtt tgtcatataa
     4861 aataaaattg gagcgctcat ggaaaccttt accgcatatg aaatcaattt cctgtcaccc
     4921 gccgagggcg aaaaactgaa aaaaccatga gtaattactg aggtataaac cagatgcacg
     4981 aactccccgt aaccgagagc atcctccgga ttgtactccg gcacgccgag gaagcgaaag
     5041 caggcaaagt catcggcatc agcctgagga tcggggagat gaccgatgtc atcgacgagt
     5101 ggcttaagcg ctatttcgat tacctgagca agggaaccct tgccgagggc gctaaactgc
     5161 gcgtcgagag gatgcccgtg atcttccgtt gcgaggtctg cggggaaaca ttcccagtca
     5221 agatccggga gatcaaggat gtcgtctgcc ctgcctgcgg gggagaaaag gcgatattcg
     5281 tttccggccg ggaattcttt atcaaagaaa ttgaggtgat ctgatgtccg aggtgcgact
     5341 aattgagatc aaagaagaga tccttgttga caacgacaag gtcgccgaaa aaatccgcaa
     5401 gcagctttgg gcaaggcagg tcttcatgct caacctcatg tcgtcgccgg gcgccggaaa
     5461 gacaagcctg atcatcgcaa ccctgcgcag tcttaatgaa tccatgcggg ccggcgtcat
     5521 cgagggggac atcgactccc tggtagatgc ggagaaagtg gctcaggagg gcatacctgc
     5581 cgtacagctc cggacgggcg gggcctgtca cctcgacgcc gtcatggttg aactggccct
     5641 gcgggagatg gatctctccg ccctcgatct catcataatc gagaacgtcg gcaatctcgt
     5701 ctgtcccgcc gagttcgaca caggcgccat gaggaaggtc atgatcctga gcgtccccga
     5761 gggtgacgac aagcccctca agtaccccct gatgttcact gtctgtgacg tcctggtggt
     5821 caacaagaca gactacctga agctttccga cttcgacatg aagaccctga aggaacgggt
     5881 tttaaagctg aatccgcaga taaagatatt tgaggtctcc tgcaagacgg gggagggcat
     5941 ggctgcctgg acgcaatggc tccgggcaga gattgagaca tttaaaaaaa tgacagagga
     6001 ggaagaccat ggacgtgtat gaaaaattac gggagatcct cgatgcgcac cccacgggag
     6061 cgcccccctc gccggtattt gacaagatct tgagaatact cttcaccccc ccggaggcgg
     6121 aacttctgac ccacatgaat ttttcaccca aaccggtgga gactattgcc gctgctgcgg
     6181 gggcgcccgt tgcggaggcc gatcgcatgc tgggcgccct ggccgataag gttctcgtct
     6241 ttggccggga aaagaacggg aagcgctcct atggtctgct gcctgccatc ccgggacttt
     6301 tcgaataccc cttcatgcgg ggagagatga cgccggaact ggtgcacctg ggcaaactat
     6361 gggaggatta ccatcgggac ggcctcggcg aatcttttgc cggaagcccc acccccgtga
     6421 cacgcgtcgt tcccgtcgaa aaatccgtcg ccgcgcccct ccaggtccat ccctacgagg
     6481 aggtcaagaa gctcatcgac agtgtggact tcatcagtct cggccagtgc gcctgccggg
     6541 tgaccctcaa acgctgtgac aagccgacgg agacgtgcct cttcttcgac gctccggccc
     6601 ggttcctcgt agagaagaaa tacgcccggc agatcacccg cgacgaggcg tatgccgtcc
     6661 ttgagcgcgc ggaagcggag ggactggttc acaccagcac gaacacggcc gaccgggtcg
     6721 gtttcatctg taactgctgc cgctgctgct gcattatttt acggggtctg acggaactca
     6781 atcttcccca tgcctttgcg ccgagtggtt ttcaggcgga agtgcaggcg gatgtgtgca
     6841 tcggctgcgg cctctgcgcc gatgaccgct gccccgtcgg cgccatcggg atgaaagacg
     6901 accttgccgt tgtcgaaacc gtaaagtgca tcggctgcgg cctctgcgta acggcctgtc
     6961 ccgtggaggc catctccctg gtcaggcgga ccgcagcgcc ggagattccg gccaccaacc
     7021 aggagatgct cgggaaactc ctgacggaaa agggcaagat agagcggttc acaaagctca
     7081 tgaagtcctg agggagccgt catgctgtcg ccggagctgg ccgcgctgac cgttaccgcc
     7141 gcttccctgg ggtttttcca taccctcctg gggccggatc actatctgcc ctttgtcgtc
     7201 atggcccggg caaggcggtg gtcccaggcc cgaaccgctt ggatcacgct gttctgcggg
     7261 cttggccaca tcggcagctc tgttgtgttg gggctcgccg gcatcaccct cggtatggcc
     7321 gtgacgagcc tggaggggat agaggccctc cggggcgacc tcgcggcctg ggcactcatc
     7381 gcttgcggac tcgtatattt cgcctggggc ctcaagaaag cctacagata tcgcccacat
     7441 gagcatagtc atttccacga gaacggtacg gcgcacaccc accggcatat ccacgggacg
     7501 gatcacgccc acgttcacgg cgataacagc agaatcaccc cctgggttct cttcatcatc
     7561 tttgttctcg gtccctgtga gcccctgctt cctgttctca tgtatccggc ggcaaaggaa
     7621 agccttgcgg gtatgttgtg ggtgacgggc gtctttggca cggtcactat cctgaccatg
     7681 atgggaatcg ttctattctc gctaaggggg ataagcctga ttccgctcaa gggaatcgag
     7741 agatacacgc acgccctggc aggggggacg atctgcttgt gtgggatcgc cattcaatat
     7801 ctgggggttt gagacggcgg agaactcaga tgggaaaatt cagatagggt acggcatcgt
     7861 cgtttcccgt cgtctgcctt tttccccgtg agacattgct gtaatggccg ttatacctta
     7921 ctatctgctc gccttttcgt tccggggaca cgttgcgatc cctggcgggc ttccgcaaca
     7981 cgtccccgat gaggagcgca tggcggccag ccattccagc gcaggaaaaa ccttgaactc
     8041 caccacgcgc ttctgatggc aggatgggca aaaatggcgg cgcttgcagg aaaaggccgg
     8101 caaatactca tggccgcagt catgacattt gacacgtgca aagccgttgt gcggatcg
//
LOCUS       DAOSMF010000083         8107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_9900_length_8107_cov_20.603080, whole genome shotgun sequence.
ACCESSION   DAOSMF010000083 DAOSMF010000000
VERSION     DAOSMF010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 8107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8107
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9900_length_8107_cov_20.603080"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..982
                     /locus_tag="PLT63_08150"
     CDS             <1..982
                     /locus_tag="PLT63_08150"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOD98733.1"
                     /translation="TMKQLAIGVIISVILVYFSLRGIDYQGLIRGLQGIAPGYAAAAL
                     AIMILMQAVRSVRWGVILSPIHKVGQFDLFAVTNVGFLAIASLPARLGEFARPLLIAK
                     KAPIKMSSALATIFIERIFDSLTIFLIFAVTMLMTPLPSWLIRSSAIFLLLTLAILSF
                     MVFMLFRRELCLRLLAPLINRLPGRYAVLADRLLHQFIDGFGMMAKPRLLAQTAALSV
                     FFWLIDAFAIYMMFKAFHLTLPFIAAFVVLVILMIGIAIPAGPGFVGNWHFFCILGLT
                     LYGVPKTEALSFAIVYHFLSIGVVIILGLLFLPANRFSLADLKNMARKPD"
     gene            complement(1185..3374)
                     /locus_tag="PLT63_08155"
     CDS             complement(1185..3374)
                     /locus_tag="PLT63_08155"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HOD98734.1"
                     /translation="MGIISKLMVLVMFLLFSGCAAMTPDQTRLHLEAEIGRSGDATEH
                     QLSQLCEAYLHLRRYDRLFECCERLENNLRKEYRGIGYSASVPYVLRAQAYIDLGQFE
                     KAIGEAKKGFDIASGEKNHIHIKIAALGLLGSAYALHEDRENAIKVINQLKSPQASTG
                     FIVQKVINIHMTNIYVALKEYQKAIDVIQQDSGIIIPEIDKIYRSFQLNKCFLETGQT
                     NKAKSGYDELLKIPQISDIGGIYWLILFDRGRIAEQEGNLREAVGFYRQAVDVIEQQR
                     STINTEADKIGFVGDKQSAYHRLISVLYRDGQYATAFEYVERSKSRALVDMLAAKKDF
                     AVRSSDELAIRDLLAENTANEAELSVQDLSLNKSELRSIVIKNRGKLREQSPELASLV
                     SVTSLATAEIQSNIPGDEVLVEYYYAEKDMIAFILSAQGLKAIKLNSDNLADDIQQFR
                     KLLDNPASSGYMESSHRLYARLIQPIESSLGHRNLIIVPHGSLHYLPFNVLHNGKGYL
                     IDHYSIRVLPSASVIKYLTAKKSSQPANILVFGNPDLGDPRYDLVYAQNEAIAVAKTQ
                     PLSKVLLRKEATETAFKKYGGGFNYIHFATHGQFNADAPLSSALWLARDAESDGMLTV
                     DKLYSTKLDADLVTLSACETGLGKIANGDDIVGLSRGFLYAGSSSIVASLWKVDDLAT
                     SDLMTRFYSTMKTKDKREALRSAQLDTMSKYPHPFYWASFQLTGNAK"
     gene            complement(3704..5110)
                     /locus_tag="PLT63_08160"
     CDS             complement(3704..5110)
                     /locus_tag="PLT63_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOD98735.1"
                     /translation="MRKTILIVDDEESICQSLAGILRDEGYETISAGSGEEALKLIEE
                     EMPNLVLLDIWLPGMDGIDTLKIIRSEYPQIRVIMMSGHGTIETAVKATKLGAYDFIE
                     KPLSLDKIILLVNHALDIIALEEENVLLKQKVTQQYELTGGSISIMELKEAIGIIAPT
                     NAWVLIMGENGTGKELVARSLHRLSRRNGKPFIEVNCAAIPEDLIESELFGHEKGAFT
                     GATQKKRGKFDLAHEGTIFLDEVADMSLKAQAKILRILQEKKFERVGGTKMIHVDVRV
                     IAATNKDLEKEMEEGRFRQDLYYRLNVIPLQIAPLRERREDIPPLVDRFLRDFAAKEG
                     EDPKTITEEALALLMDHDWPGNVRELKNIVERLVIMTPGQAITAADIPLFSKESGHGL
                     ESKPGLGARTPALDSESLRAARMDFEKQYILRKLRENDGNISRTAEAIGLERSNLHKK
                     IKSYGLDVDRQEGTGGRQ"
     gene            complement(5103..7361)
                     /locus_tag="PLT63_08165"
     CDS             complement(5103..7361)
                     /locus_tag="PLT63_08165"
                     /inference="COORDINATES: protein motif:HMM:NF039586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD98736.1"
                     /translation="MTRDRSAPLLDVKELRRRKRERILIVVTVILVVLLTFLETHLTK
                     QETILPISSNVLIFGLININIILIILLIFLIMRNLVKLVYERRHGVIGSRIRTKLVAA
                     FVSLTLIPTFVLFLVSINFLSYSIENWFNIRIGDALNSTLEMAQTSYKYTSDYAKFYA
                     RQISGDITESRLYEKERGKYLRTLVEQRQKAYNLGLLEVHFDSMREKVIVQDSQNSHI
                     VPLNLPPNLVEEVFAGKEVVAEHSSGSGNIISGVAPIYSLLATGEVVGMVAVGYYIPR
                     AFVDKMAVISKTSEEYKQLSLLKNPIKFTYIVTLSIVTLLIVFSATWFGLFLAKQITV
                     PIQDLADATGRIARGDLDYRIDIAADDEIGTLVDSFNSMTKDLKKSKQNLELANLDLE
                     QRRKYMEAVLSNVSAGVVSVDKHDVITIINRAAERMLEIKTEKVLNRPYQEVLSAEHL
                     ALVKELLGELKESENGFVERQLAITLKDRFLTILITTTVIRDDEGNYLGMVLVFEDLT
                     QLQKAERAAAWREAARRMAHEIKNPLTPIQLSAQRLQRKYGDRLQEDGDVFQECTKTI
                     IGQVEVLKNLVNAFSRYARMPVTNPSLNDLNEVLGDAIVLFQDAHKDIAFTFQKGEAI
                     PSLHIDPEQIKRVMLNLLDNAVAALGLEERRIEVRTGYDAQRRRAVVEVADNGVGVPA
                     KFKSKIFEPYFSMKRAGTGLGLAIVSAIIAEHRGQVGVRDNKPRGTVVTFELPAPEAE
                     AAANHAKKGDYA"
     gene            complement(7358..7969)
                     /locus_tag="PLT63_08170"
     CDS             complement(7358..7969)
                     /locus_tag="PLT63_08170"
                     /inference="COORDINATES: protein motif:HMM:NF025692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HOD98737.1"
                     /translation="MQLRFLFLWPSAKFGEKTLSRLIITILFIMGSWLCPAGSAAREA
                     QIGDVLVARQPDRFTVFARVTNCFTSGMESAIMAGVPTTFTFLVDFYRERNFWPDKKI
                     AAVVVRQTVKYDNVKKIFLVSMDHNGNPTAFQDLEGAKRAMAELTAHIAIPRNVDRNR
                     GTYYLLIKAKLDKVRLPLYMEYVFFFVSLWDFETDWYRKEVAF"
BASE COUNT         1715 a         2247 c         2112 g         2033 t
ORIGIN      
        1 aactatgaaa caactagcta tcggcgtcat catcagcgtc atcctggtct atttctccct
       61 gcggggcatc gactatcaag gcctgatccg ggggctccag ggcatcgcgc cgggatatgc
      121 cgcggcggcc ctggccatca tgattctcat gcaggcggtc cggtccgtcc gctggggggt
      181 tatcctcagc cccatccaca aagtcggaca gttcgacctc ttcgccgtaa ccaacgtcgg
      241 ttttctggcc attgcctccc tgcccgcccg gctgggcgaa ttcgcccgtc cgctcttgat
      301 cgccaaaaag gcccccataa agatgagctc cgccctggcc accatcttca tcgagcggat
      361 cttcgacagc ctcacgatat tcctgatctt cgccgtcacg atgctgatga cccccctgcc
      421 ctcctggctg atccgctcca gcgccatctt tctccttttg accctggcca ttctaagctt
      481 catggtcttc atgctcttcc ggcgcgaact gtgtctgaga ctgctggcgc ccctgatcaa
      541 ccggctgccg gggcgctatg cggtcctggc ggaccggctc ctccaccaat tcatcgacgg
      601 cttcgggatg atggccaaac cgcgcctgct cgcgcagacg gcggcgttgt ccgttttttt
      661 ctggctcatc gatgcatttg ccatctatat gatgttcaag gcctttcacc tcacccttcc
      721 gttcatcgcc gccttcgtcg tcctggtcat cctcatgatc ggcatcgcca tccccgcggg
      781 tcccggcttt gtgggcaact ggcatttctt ctgcatcctg ggccttaccc tttacggcgt
      841 gccgaagacg gaggccctct cctttgccat tgtctatcac ttcctgtcca tcggcgtcgt
      901 aattatcctg ggtctactgt tccttcccgc aaaccgtttc tcccttgccg acctgaagaa
      961 tatggccaga aaacccgatt gaacctctta tcccgaataa gtattttccg cgctccgttc
     1021 ttccgtaaaa aacggggagc actgtcgtca tttcacctat tatattataa agttatcctc
     1081 tgccggagca ccggcggaca tttttccccg gcggaaaacg atcccaagaa ctccatatgc
     1141 tccatcctcg ccggtcctgg gaggcctacc aacaatagtt ctgtttattt cgcattcccc
     1201 gtcagttgaa atgatgccca gtaaaatgga tggggatatt tactcatggt gtctaactgg
     1261 gcagaccgga gagcttcgcg cttgtccttt gttttcatag tggaatagaa ccgcgtcatc
     1321 agatcggagg tggcaagatc atcaactttc caaaggctcg caacaatgga actgcttccc
     1381 gcatagagaa accccctcga caatcctacg atatcatcac cattggcaat cttgcccaaa
     1441 cccgtttcgc aggcacttaa agtcaccaga tcggcatcaa gttttgtgga atagagtttg
     1501 tccaccgtca gcatcccatc actttcggca tctctggcca accagagggc agaacttaag
     1561 ggtgcatctg cattgaactg tccatgggtc gcaaaatgta tatagttaaa tccgccgcca
     1621 tattttttga atgccgtttc cgtcgcttct ttacgcaaca ataccttgga tagaggctgt
     1681 gttttggcaa cggcgatggc ctcattctgg gcatagacaa ggtcatatcg gggatcaccc
     1741 agatcgggat taccaaatac aaggatgttt gcgggttggg aagatttttt agccgtcagg
     1801 tacttgatca cactggcact cggcaggacg cggatgctgt aatgatcaat gagatacccc
     1861 ttcccattat gcagcacatt aaagggaaga tagtgcaatg aaccatgggg aacaatgatg
     1921 agattgcgat ggccgaggga gctttctatg ggctgaatga gtctggcata caggcggtgg
     1981 gaggattcca tgtaaccgct tgaggctgga ttatcgagga gtttgcgaaa ctgctggatg
     2041 tcgtcggcaa gattatcgct gttcagctta atggctttca atccttgggc agacaggatg
     2101 aaagcaatca tgtctttctc ggcatagtaa tactcgacaa ggacttcatc cccggggatg
     2161 ttcgattgga tttctgcggt agcaagtgac gtgacactaa caagggatgc cagttcaggt
     2221 gactgttccc tcagttttcc cctgtttttg atcacaatgc ttcgcagctc cgatttgttc
     2281 aaggataggt cttggacgct cagctccgcc tcgttggcgg tattttctgc aaggaggtca
     2341 cggatcgcca gttcgtcact gctccggacg gcgaaatcct ttttggctgc cagcatatcg
     2401 accagggcgc gcgatttgga acgttccacg tactcgaaag ccgtggcgta ttggccatcc
     2461 cgatacaaaa ctgaaatcag acggtggtaa gcgctctgct tatcccctac gaaaccgatc
     2521 ttgtctgcct ccgtattgat ggtagaacgc tgctgttcaa tgacgtcgac cgcttgacgg
     2581 tagaagccga ctgcctcccg gaggtttcct tcctgctcgg ctatacgtcc ccgatcaaag
     2641 agaatcaacc agtagattcc gccaatatca cttatttgtg gtatttttaa aagttcatca
     2701 tatccggatt ttgctttgtt ggtctggcct gtctccagga agcacttgtt tagttgaaat
     2761 gatcgataga tcttgtcgat ttctggaata attatccctg aatcttgctg gatgacgtcg
     2821 atcgcttttt gatattcttt tagagcaaca taaatgtttg tcatgtgaat gtttataacc
     2881 ttttgaacta tgaatccagt ggacgcttga gggctcttta gttgattaat tactttgatt
     2941 gcattttctc tgtcctcatg tagggcataa gctgatccaa gaaggcccaa tgcagcgatt
     3001 ttaatatgta tatgattctt ttccccggac gctatatcaa agcccttttt ggcttctccg
     3061 atcgccttct caaactggcc aaggtcgata taggcttgcg ctcggagcac ataaggcacc
     3121 gaagctgagt agccaatgcc tctatactcc ttccggagat tgttctctag ccgttcacaa
     3181 cattcgaaaa gtcgatcata tcgccgcaaa tgtaaatagg cctcacacag ttgtgacagt
     3241 tggtgttcag ttgcatcacc ggatcggccg atctcagctt ccaaatgtag acgagtctga
     3301 tccggagtca tggcggcaca gccagagaaa agtaaaaaca taactaagac catcaatttt
     3361 gaaataatgc ccatgctacc ccccctctct tctcgttgag aacgtggctt ttgccagcac
     3421 gttcccctta cagcgtattc atacgggatg gaactttgat tgctgttcta cgctatccgt
     3481 tcttccgtaa aaaacgggga gcactgtcgt catttcacct attatattat aaagttatcc
     3541 tctgccggag caccggcgga catttttacc cggcggaaac cgagcccaag aactccatat
     3601 atttggcggt atcggcgcta ccagcgtcta cataaaaaag gagacggttc tcttttccaa
     3661 accatcccca atcagatcca acccccgtag cctttcccac cgcctattgt ctgccgcccg
     3721 tcccctcctg tctatccaca tcaaggccgt agctcttgat ctttttatgg aggttgcttc
     3781 gttccagacc gatggcctcc gcggtccggg agatgttgcc gtcgttttcc cggagtttgc
     3841 gcaggatgta ctgtttttcg aaatccatcc gtgccgcccg gaggctttcc gaatccaggg
     3901 cgggagttcg cgcgccgaga ccgggtttac tttccagccc atgcccgctc tccttggaaa
     3961 acaaagggat atcggcggcc gtaatggcct ggcccggggt catgatcacc agacgctcga
     4021 caatgttctt caattcccgg acgttgcccg gccagtcgtg gtccatgagc aacgccaggg
     4081 cctcctcggt tatcgtcttg ggatcttccc cctccttggc ggcaaaatct ctgaggaaac
     4141 gatctaccag ggggggaatg tcctcgcggc gttcccgcag gggagcgatc tggaggggaa
     4201 tgacattgag gcggtagtag agatcctggc gaaatcgccc ttcctccatt tccttttcca
     4261 gatccttgtt ggtggcggcg atcacccgga catccacatg gatcatcttg gtccccccca
     4321 cccgctcgaa cttcttctcc tggagaatcc ggaggatctt ggcctgggcc ttgaggctca
     4381 tgtccgccac ctcgtcgagg aagatggtgc cttcatgggc caggtcgaat ttcccccgct
     4441 ttttttgcgt cgccccggta aaggcccctt tctcatggcc gaatagttcg ctttcgatga
     4501 gatcctcggg aatggccgca cagttgacct cgatgaaagg tttgccgttg cgccggctca
     4561 agcggtgcag ggatctcgcc accagttcct tccccgttcc gttttcgccc atgatgagga
     4621 cccaggcgtt ggtgggcgcg atgatgccga tcgcctcttt gagttccatg atggagatgc
     4681 tgccgccggt cagttcgtat tgctgggtca ccttctgctt gagcaggacg ttttcctcct
     4741 ccagggcgat gatgtccagg gcgtgattga ctaagagaat tatcttgtcc agggagagcg
     4801 gtttttcgat gaaatcgtag gcccccagct tggtggcctt gacggccgtt tcgatggtgc
     4861 cgtggccgga catcatgatc acccggatct ggggatattc ggagcggatg atcttgaggg
     4921 tgtcgatgcc gtccatgccg ggaagccaga tgtctaaaag gaccagattc ggcatttcct
     4981 cctcgatgag cttcagtgcc tcctccccgc tgcccgccga gatcgtttca taaccctcgt
     5041 cccggaggat gcccgccagg gattggcaaa tgctctcttc atcatcgaca atgaggatcg
     5101 tcttacgcat aatctccttt cttggcatgg ttcgccgctg cttctgcttc cggcgcgggc
     5161 agttcgaagg tcacgacggt cccccgtggt ttattgtccc gaacgcccac ctggcctcga
     5221 tgctcggcga taatggcgct gacgatggcc agacccagcc cggtgccggc ccgtttcatg
     5281 gagaaatagg gctcgaagat cttgctcttg aattttgccg gcaccccgac gccgttgtcg
     5341 gccacctcca cgacggcgcg gcgcctctgg gcatcatagc cggtccggac ctcgatccgg
     5401 cgttcttcca gccccagggc ggccacggca ttgtcgagga ggttcagcat cacccgcttt
     5461 atctgctccg gatcgatatg gaggctggga atcgcctccc ccttttggaa ggtgaaggca
     5521 atgtccttgt gagcatcctg gaaaaggaca atggcatcgc cgagaacctc gttcaagtcg
     5581 ttcagggatg gattggtcac cggcatacgg gcgtaccggg aaaaggcgtt gaccaggttc
     5641 ttgaggacct ccacctgtcc aatgatcgtt ttggtgcatt cctgaaagac atcgccgtct
     5701 tcctgaagac gatccccata tttccgctgg agacgctggg cggacaactg aataggggtc
     5761 aggggatttt tgatctcgtg ggccatgcgg cgtgcggcct cccgccaggc cgccgcccgt
     5821 tccgcctttt gcagttgggt aagatcctcg aacaccagga ccatccccag ataattgccc
     5881 tcgtcatccc tgatgaccgt ggtcgtgatg aggatggtga ggaaacggtc cttgagggtt
     5941 atggcaagct gccgctccac gaatccgttt tcactttctt tgagttcccc cagcaactcc
     6001 ttcaccagcg ccaggtgttc ggcgctcagg acttcctgat agggacggtt caggaccttc
     6061 tccgtcttga tctccagcat ccgttccgcg gcccggttaa tgatggtgat tacgtcatgt
     6121 ttgtccactg aaaccacccc agcggaaacg ttgctgagaa ccgcctccat gtatttccgt
     6181 cgctgttcga gatcgagatt ggccagttcc aggttttgct tacttttttt gaggtccttg
     6241 gtcatggaat tgaaggaatc cacgagtgtc ccgatctcat cgtccgcggc aatatcgatc
     6301 cggtaatcga gatcaccccg ggcgatgcgg cctgtggcgt cggcgagatc ctggatgggt
     6361 acggtgatct gtttggccaa aaagaggccg aaccaggtcg cggaaaaaac aatgaggagg
     6421 gtcacgatgg acagggtcac gatataggtg aatttgatcg ggttcttgag aagactcaac
     6481 tgcttgtact cttctgatgt ctttgatatt actgccattt tatccacaaa agcgcgggga
     6541 atgtaatagc ctaccgccac catgcccacg acttcaccgg tggccagcag actgtagatg
     6601 ggggcgacgc cactgatgat gttgccgctg ccagaagagt gttccgccac gacctccttc
     6661 cccgcaaaca cctcctcgac aagattcgga ggcaggttca gcggaacgat atgggagttt
     6721 tgcgagtcct ggacaatgac cttttcccgc atactatcga aatgcacttc caacagacca
     6781 aggttgtagg ccttctgccg ctgttccacc aaggtgcgca gatactttcc ccgctccttt
     6841 tcgtaaaggc ggctttccgt aatgtccccg ctgatctgac gggcataaaa cttagcgtaa
     6901 tcggaagtat atttatacga ggtctgggcc atttccagcg tgctgttcag agcgtcgcca
     6961 atgcggatat tgaaccagtt ctcgatgctg taagacagga aattgatgga taccagaaac
     7021 aggacaaagg tcggaatgag ggtcaggctg acaaaggccg ccaccagctt ggtccggatc
     7081 cgggaaccga tgaccccatg gcggcgctcg taaaccagct tgacgaggtt cctcatgatg
     7141 agaaatatta acaggatgat caggatgata ttgatgttga tgaggccgaa gatgaggaca
     7201 ttgctggaga taggcaggat agtttcctgc ttggtaagat gggtctcgag aaaggtgagc
     7261 aggacgacta ggatgaccgt gacgacgatg agaatacgct cccgcttccg gcggcgcagt
     7321 tcctttacgt ccaaaagggg cgcggaacga tcccgggtca aaaggccacc tccttacggt
     7381 accagtccgt ctcgaaatcc cagagggaga cgaaaaagaa tacatattcc atatacaggg
     7441 gaagccggac cttgtcgagc ttggccttga tcaaaagata ataggtgccc cggttgcggt
     7501 cgacatttcg gggaatcgct atatgggccg tcagctccgc catggcccgc ttggcgcctt
     7561 cgaggtcttg aaaagcggtg ggattaccgt tgtgatccat ggaaacgaga aagatctttt
     7621 taacattgtc gtatttgacg gtctgccgga ccaccacggc ggcgattttt ttgtcgggcc
     7681 agaaattgcg ttcccggtag aaatcgacga gaaaggtaaa agttgtaggc accccggcca
     7741 taatcgccga ttccatgccg ctggtgaaac agttcgtcac gcgggcaaat acggtgaatc
     7801 tatcgggctg tctggcaacc agcacgtcgc cgatctgggc ctcccgggcc gcggatcccg
     7861 cggggcacag ccaggagccc atgatgaata atatcgttat aatgagtcgt gacaatgtct
     7921 tttccccgaa cttcgccgac ggccatagga aaaggaagcg gagctgcatg catgatatcc
     7981 ggatcgagtt gccaaaccca tttgccgacg gcggtgcaac tgcggccacg ccatcctccc
     8041 tgagagcctg atctgctgcc tgatggaacc tggaagagca tgaattaccg atcaccatga
     8101 tggctcc
//
LOCUS       DAOSMF010000084         7940 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_10202_length_7940_cov_15.033989, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000084 DAOSMF010000000
VERSION     DAOSMF010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 7940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7940
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10202_length_7940_cov_15.033989"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="PLT63_08175"
     CDS             <1..408
                     /locus_tag="PLT63_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98738.1"
                     /translation="YDELAEAMELTWEERQSRSYRSLLGKVRGMNDLDSLRALGKKVY
                     ALGLSRDQAGVLWYEYRKTERRIMAEIGKSLSVSARRLLQQVRNANGNLAALGARLFK
                     IQRGTVKMADPPRKHEWIVIWAAYHDRQAALRP"
     gene            573..782
                     /locus_tag="PLT63_08180"
     CDS             573..782
                     /locus_tag="PLT63_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HOD98739.1"
                     /translation="MRTYTAVIEKCPDTGLFVGYIPGMRGAHSQGETLDELNRNLKEV
                     VELLLEDGEPELEAEFVGTQSVVVA"
     gene            complement(1317..1484)
                     /locus_tag="PLT63_08185"
     CDS             complement(1317..1484)
                     /locus_tag="PLT63_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98740.1"
                     /translation="MNLYDDEIYDHKMEEIVVHLEYFLLDLMRVAAKRLIDNNADLFA
                     NSQPQTSNDDF"
     gene            complement(1503..2312)
                     /locus_tag="PLT63_08190"
     CDS             complement(1503..2312)
                     /locus_tag="PLT63_08190"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOD98741.1"
                     /translation="MNDSYRTFFGLTKEPFSAELGIKELLETPDLKAVKDRFDYAVRL
                     GAVALVTGEIGSGKSTALRYAAGHLHTSEYCTLHVTATSGSITEIYRQLLDELGASNV
                     SFSRALLLKRIRSEIMELVQGKKINVVLLIDEASLLRLEVLTELHTLCQFEQDSKPWL
                     PLILAGQSNLVDKLMYRTSLPLASRIVARSHLEGVNRQGMGDYLKHHLAIAGVKTTLF
                     DDTAVTAIHQGSGGLFRKANHLARGALIAAAHDQSMTVSAHHVRLASTEIF"
     gene            complement(2305..3609)
                     /locus_tag="PLT63_08195"
     CDS             complement(2305..3609)
                     /locus_tag="PLT63_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HOD98742.1"
                     /translation="MTEEEKKRVAVFRFGVIYDLINGGDLEKGQHAQLLRQKCARKWE
                     IPHSGKTRIGRSTILRWIRLYRLSNGKIESLYPDDRSDRGESRVLDGETALSLIRLRQ
                     GMPQATAAVLIKTMEERGMVAPGKKLHLSSVYRFLHRHGIMKAKGRQPEDRRKFESEL
                     PNDMWQSDVMHGPLVEAGGKMKKSYLIALLDDHSRLVAHGEFYLAEGISSYVDALEKA
                     LLKRGLPRKLYVDNGPAFRSLHLDHIAASLGIALIHSRPYKPEGRGKIERFFRTVREE
                     FLTGFRGGALADLNEAFDAWLTDVYHARKHSSTGQTPFVRFTANVACLRAAPKDLRDH
                     FRKVARRNVAKDRTLTLDGKLYEAPVALIGRRVEVLYHENDPDRIEIRSEKQSYGIFM
                     PVDIHVNCRVKRCADRTDLAVAEGRSYQGGRLWSGRGEVPHE"
     gene            5176..5418
                     /locus_tag="PLT63_08200"
     CDS             5176..5418
                     /locus_tag="PLT63_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98743.1"
                     /translation="MKKLSPYIEHCLNCAITGQDPNPDDKFYRKLELWDEIINTHVWG
                     MNAALRISQQAEEAMRIKARENNDNDSNEQERRNES"
     gene            5415..5822
                     /gene="ssb"
                     /locus_tag="PLT63_08205"
     CDS             5415..5822
                     /gene="ssb"
                     /locus_tag="PLT63_08205"
                     /inference="COORDINATES: protein motif:HMM:NF012651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOD98744.1"
                     /translation="MINTVTIVGNLGQDPEIRYTASGVPVAGFTVAVNEIYKGKDGQK
                     VKKVHWFRVTAFQRLAEIVGEYCTKGVKVGVHGQLQQRTWQGEEGFNRSIVEIRAREL
                     ELLGGRNGNQSKPEGDGPSERTSLFNEDDDVPF"
     gene            complement(5896..6210)
                     /locus_tag="PLT63_08210"
     CDS             complement(5896..6210)
                     /locus_tag="PLT63_08210"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HOD98745.1"
                     /translation="MNAMRPIHPGEVLKEELAEINMTANAFAKALHVPANRITAILNG
                     TRSITADTALRISIFFGTTPEFWLNLQTAYDLKVAKNSVGEKVKQEVRSFIPAIPCLK
                     HA"
     gene            complement(6224..6502)
                     /locus_tag="PLT63_08215"
     CDS             complement(6224..6502)
                     /locus_tag="PLT63_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005936145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOD98746.1"
                     /translation="MIRSFACKHTEKLYNGGRVPRFQSIMQQAERRLQILDSATCIED
                     LMNLPSNRFESLGGDRTGQQSIRINQQWRICFEWREDGPYNVEIVDYH"
     gene            complement(7231..7524)
                     /locus_tag="PLT63_08220"
     CDS             complement(7231..7524)
                     /locus_tag="PLT63_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOD98747.1"
                     /translation="MPYNLTFKESALKEWGKLDRPIREQFKKKLAKRLERPRVETDRV
                     SGMDDAYKIKLRAVGLRLVYVVEECTKTLTVYAVGKRDKKEAYRKAKDRINIT"
     gene            complement(7514..7759)
                     /locus_tag="PLT63_08225"
     CDS             complement(7514..7759)
                     /locus_tag="PLT63_08225"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HOD98748.1"
                     /translation="MDYLVAKSSVSITDLKKNPSAVIKEAEGAPVVILNHNRPSAYIV
                     PAETFEAMLEKLDDLEIKKTIRKRRGEKSVPVSLDAL"
BASE COUNT         1778 a         2229 c         2046 g         1887 t
ORIGIN      
        1 tatgatgaac ttgccgaggc catggaattg acctgggaag agaggcaatc acgatcatat
       61 cgttccttgc tgggcaaggt tcgcggcatg aatgatcttg attccctccg ggctctcggc
      121 aagaaggttt atgcccttgg cctctcccgt gatcaggccg gggtgctctg gtatgagtac
      181 cggaaaacgg agcggcggat catggccgaa atcggcaagt cactcagtgt ctccgcccgg
      241 aggctattgc agcaggtccg gaatgccaac ggcaatctgg ccgccctggg cgccaggctc
      301 tttaagattc aacggggcac ggtcaagatg gccgatccgc cccggaagca tgaatggatc
      361 gtcatctggg ctgcctatca tgatcgccag gcagccttgc ggccatgagg gccattgaac
      421 cctgggtttc tgtttgattc cttattggtc cagacagaaa ttcaggagtg ttaaaaattc
      481 cttacttctt gacatttccg aggttatcat acattttatg actgcaaatc gatttttatc
      541 aggatagacg tgcaaaaaga aggaggcgta atttgagaac atataccgcc gtaatcgaga
      601 agtgtccaga tacggggctc tttgtcggct acattccggg aatgcgcggc gctcattccc
      661 aaggagaaac cttggatgag ttgaaccgta atctgaaaga ggtcgtggag ctgctcttgg
      721 aagatggtga gccggaactg gaggctgaat ttgttggcac gcagagcgtg gtcgtggcct
      781 gagcatggga tcgcgtgctg tactcaaacc agcggaggtt attgctattc tcgtaagaat
      841 ggggtttagc gaatcccgcc agaagggaag ccacaaacaa ttccgccacc cggatgggag
      901 agggacaacc gtaccttgcc atccgggaag agacatctca cctatcctgt tgcgtcagat
      961 tgcccgagac atcggcttga ccatcgaaga attcctgaaa gaacgctaat cccacccacc
     1021 gcccccttaa agcagtgagc aaacaccgcg ccagacaaac acgctgtcac gccgtttgca
     1081 gcatctcttt tcccgcccgc ccaaaccctt ttccggctgt catgccgcct gcatagctgc
     1141 gtacaagaca tgaccggcta agccgccatg ccctgtgact cgcgacaggc atcccgcctg
     1201 tagattttca gattagccgg tcagtaatag tctgattata tgggactgga ttgccggttt
     1261 tcggattagg aagaacggga cgcctgtttg tgagcgcccc gttcttcctc tttttatcag
     1321 aagtcgtcgt tactggtttg cggttggctg ttcgcaaaga gatcggcgtt attgtcgatg
     1381 agcctttttg ccgccacccg catgagatcg aggaggaagt attcgagatg gaccacgatt
     1441 tcctccattt tgtggtcata gatttcatcg tcatagagat tcatgttgtt ttccttttct
     1501 tatcagaaga tttccgtgga agccaggcgg acatgatgtg ccgagacggt catggattgg
     1561 tcatgagcgg cggcaataag ggcgcctctg gccagatgat tggcctttct gaacaggcca
     1621 ccggaacctt gatggatggc cgtgacggcc gtgtcgtcga agagggtggt tttcacgccg
     1681 gcgatggcca ggtgatgttt gagatagtct cccatccctt gccggttgac gccttccaga
     1741 tggcttctgg cgacaatgcg cgatgccagg ggcagggagg tccgatacat cagcttatcg
     1801 acgagattgg actgcccggc taagatcaag gggagccagg gtttggagtc ctgttcaaac
     1861 tggcagaggg tatggagttc cgtcaacacc tccagccgca gcagggatgc ttcgtcgatc
     1921 aacagaacga cattgatctt tttgccctgc acaagttcca tgatttcact gcggatgcgc
     1981 ttcagcagga gggcccggga aaaactgacg ttgctggcgc ccaactcatc gagaagctga
     2041 cggtagattt cggtgatgga tcccgaagtg gccgtgacgt ggagcgtgca atactcggag
     2101 gtgtgcagat gaccggcggc atatctcaaa gcggtggact tgccgctgcc gatctccccg
     2161 gtgacaaggg ccacggcgcc cagacgaacg gcatagtcga aacggtcttt gaccgcttta
     2221 agatccggag tttccaggag ctccttgatg ccgagttcgg cgctgaaggg ttctttcgtc
     2281 aggccgaaga aagtgcgata ggaatcattc atggggaacc tcccctctgc cggaccagag
     2341 acgtcctccc tgataagagc gaccctcagc gacggccaga tcggtcctgt cggcgcatcg
     2401 cttgacccgg caattcacat ggatatccac cggcatgaag attccgtatg actgcttttc
     2461 cgagcggatc tctatccggt ccgggtcgtt ttcatggtac aggacctcca cccgcctgcc
     2521 gatcagcgcc acgggggctt catagagttt cccgtcgagg gtcagggtcc tgtctttggc
     2581 cacgttgcgt ctcgccacct tgcggaaatg atcccggaga tctttcgggg cggcccgcag
     2641 acaggcgaca ttggcggtaa aacgtacaaa cggggtctgt ccggtggagc tgtgcttccg
     2701 ggcatgatag acatcggtca gccaggcgtc gaaggcctcg ttcagatcag ctagggcgcc
     2761 gcccctgaat ccggtgagaa actcctcccg gaccgtccta aagaaacgtt cgatcttccc
     2821 ccgcccttct ggtttgtacg gcctagagtg aatcagggca atgcccaggg aggcagcaat
     2881 atgatccagg tgaagggaac gaaaggcggg accgttgtcc acgtatagtt tccgcggcag
     2941 cccccgcttc agcagggctt tttccaaggc gtcgacatag gacgagatgc cctccgcgag
     3001 ataaaactcc ccatgggcaa ccagccggga atggtcatcg agcagggcga tgagatagct
     3061 tttcttcatc ttgccccccg cttcgaccag cggtccgtgc atcacatcgg actgccacat
     3121 atcgttgggc agttccgact caaacttgcg acggtcttcc ggttgacgcc ccttggcctt
     3181 catgatgccg tgccgatgga ggaaccggta aaccgaagag agatgcagct tcttccccgg
     3241 agccaccatc ccccgctctt ccatcgtttt gatcagaacc gccgccgttg cttgcggcat
     3301 cccctgccgc aaacggatca gggaaagcgc cgtttcacca tccagcaccc ggctctcgcc
     3361 ccggtcggaa cgatcatcgg gatacaatga ttcaatcttc ccgttgctca gtcgatacag
     3421 ccggatccac cgcaaaatgg tgctgcggcc gatccgggtc ttccccgaat gggggatctc
     3481 ccacttccgg gcgcacttct gccggagcaa ctgggcatgc tgcccttttt ccagatcgcc
     3541 cccgttgatc agatcgtaaa tcacaccgaa tcgaaagacc gccacccgct tcttttcttc
     3601 ttccgtcaca gagaatctcc tttcttggtt tttgaagacc tctacaacgg aacgtctctt
     3661 ccggcgactg aacccttcgg tagggtccgt caaaacggcg gcatccggca ggctatatgg
     3721 aacggctcac ggggatccgg ccgacgccca tcaaataatc gaaggcgtca agaagattcc
     3781 ccttccaggt gttatccaag tatgcccaaa ccctcctctt caacgccctc aaccaatgac
     3841 cttggcaagg agaggacttc ccgggcgccc aacgtccttc catcaatcgg aaggacaatc
     3901 tggagcgaat ctccgccaca gatgactgaa atcgccgaaa atacccgcag ggcctcatcc
     3961 gtatcacgca ccggcaggac ggacaccgcc aacgccttaa atatacaaac ccggaaaccc
     4021 catcaaaata cgccgtcaca aatccatgtc cccatacatg actcgcacgg cagcagggac
     4081 aacgctccgg ccttgaccac tcatactccc gccccttctc aaaaatctcc ttgacctcgc
     4141 acggaacact gattaccatc tccacacctc atatgggatt ctcactgtct tctctatgag
     4201 ccaagggcgg tgtctcttcc gaaaagtcca ccgcccttcc ataaaaaaac ctctctcccg
     4261 agaggttcat ctaatccgat aatcccaacc gacctgtcaa tctattctga aaataacacc
     4321 ccttttctta accacataat atgacaatct acaccgccat ccttatcccc cgcccgccct
     4381 ggcggtctcc ttcgcccgat ggtgtcatgc tgcgcatcac ctcatcgggc ttgtcgggcc
     4441 cgctcccatc cgcccccagg ggctccccct tgtttcgttg tgccccagag ccgtcacagc
     4501 ccctcgcttc gcgacgggac tgtcacgact ctggaattcc tgtttctttc ttcgttcgca
     4561 ccggctacca tgcgacctca ctttgctcag acgctcggaa gccgggctca ctttcaagtg
     4621 tttacagtac gcccgcttgc ccctgacgcc tgacggcgac aggaggcttc gggccgcctt
     4681 cttatttctc tccggggcgt cgcagacgat cgctgcgcgg cgactgcttc gcccctgcga
     4741 cttatcgaac cttttctatc ttttcgcctc ggcccggcag acttcgctcc gctcagactg
     4801 cccaggccgg ccgttatcac agccttcgcc gtgacccgta ccgcgcttcg ccaggaacgg
     4861 gacacggctt ccggctgctt ttcctgctgg tgctccggga ggcagcagac acttgctgcg
     4921 ccgtgactgc cgcgtccctc cggtctttat tcaaactctg cccgcatccg gccggcctcg
     4981 cttccctcgg acgcccggaa gcagggcttc tctgaatggt attgccctat cgcctcctgt
     5041 cacctcctga agtcggagac atgaggctgc gggcatttct ttctctcctc gtcggggcga
     5101 cacatgcatg acgcccctcc ttaaataaat ttcatcagag ctgtcacagg gcaaaatact
     5161 ttgaggaggt cggctatgaa gaaactgtca ccctatatcg aacattgtct caattgcgcc
     5221 atcacgggcc aggacccgaa tccagacgac aagttctacc gaaagctgga gctctgggat
     5281 gaaatcatca acacccatgt ctgggggatg aatgcggctc tgagaatctc ccaacaagcg
     5341 gaggaagcga tgagaatcaa ggcaagggaa aacaatgaca acgacagcaa cgaacaagag
     5401 aggaggaatg aatcatgatc aacacagtga ctatcgtagg gaatctgggg caggatccgg
     5461 aaatccggta taccgcgagc ggcgtccccg tcgccggctt caccgttgcg gtcaatgaaa
     5521 tttacaaggg caaggacggc cagaaggtca agaaggtcca ctggtttcgg gtgacggcct
     5581 tccagcgcct ggcggagata gtcggcgaat attgcactaa gggcgtcaag gtcggcgtcc
     5641 acggccagct tcaacagcgc acctggcagg gcgaagaggg cttcaaccgg agcatcgtag
     5701 agatccgggc cagggaactg gaactcctgg gcggcaggaa cggaaatcag tccaagccgg
     5761 aaggggatgg gccatcggaa cggacttcct tatttaacga ggacgatgat gtcccgttct
     5821 gaaacaaaaa atgatctcgg cgatacttcc ggaaaagaca taacggcgca ttgggtgacg
     5881 ccaatgcacc gttatttagg cgtgtttcag gcaaggtatg gcaggtataa aagatctgac
     5941 ctcttgcttt accttttcgc cgacagaatt ttttgccacc ttcaaatcat acgctgtctg
     6001 aagattcagc cagaattccg gcgtggttcc gaaaaaaatg gaaatgcgca gcgcggtgtc
     6061 ggcggtaata gagcgggtac cgttcaggat ggctgttatc cggttggccg gcacgtgcag
     6121 tgccttggca aaggcattgg cggtcatgtt gatttcggca agctcttcct tcagcacttc
     6181 gccgggatgt atggggcgca tagcgttcat ggcgttcctc ctttcaatga taatctacga
     6241 tttctacgtt gtatggaccg tcttcccgcc attcgaagca aatacgccac tgctggttta
     6301 tgcggatgct ctgctggccg gtccggtctc ctcccagtga ttcaaagcga ttactgggaa
     6361 gattcatcag gtcctcgatg catgtggcgc tgtcgagtat ctgtaatcga cgctcagcct
     6421 gctgcattat ggattgaaaa cggggcactc ttccaccgtt gtaaagcttt tcggtatgct
     6481 tgcaggcaaa tgatcgaatc atggcttgca ggttacactt gtttacgttt tacgtcaagc
     6541 gtaaatattt agatatttgg ttgataaaga ggggagagtg tcgggaccga cctatacaat
     6601 catttgagaa tttacatgca ccagcaatga catgcgcagc agctaatgaa aaggcacccc
     6661 atgtaaaacg cgggatgcct tttctgttga tttggttttg attcaccgtc cgctgcgaat
     6721 aatgaccttt gggttgtttt ggaaaatctg tagaaaaaat gtttctaacg gatttctaac
     6781 gtaaccccct caaaaacatt tttccctgtg ttatggtgaa acccaagaag taatcataac
     6841 ctcttgatcg acttgagata acctcaaatt atgtttatgt tactatattc ttactattct
     6901 gctcataacc caaaggtcag aggttcgaat cctctccccg ctaccattaa tatcaagggg
     6961 ttagccaata aatggttagc ccctttttct tttttctacc ctatttatac ccattggcgg
     7021 taattggagg gaaatcagag gtagcgagtc cccttttttg atcccgtcgt tgagatagaa
     7081 tttctcaacc tcactctcaa aatagccctg tgggacaaat tcgcgcaagt cctctaagat
     7141 ctgtgctcgc agttgtttta caatccacca atccatgcca tctccgtcat tttgtgctct
     7201 taactgagaa ccatttccaa taggatattg ctacgttata ttgatcctat ctttagcctt
     7261 gcggtaggcc tccttcttat cccttttccc cacggcatag accgtcaagg tcttcgtgca
     7321 ctcttccaca acataaacca gccgcagacc gacggcacgg agcttgatct tgtaagcgtc
     7381 atccataccg ctgaccctgt ccgtttccac gcgcggacgt tccagccgct tcgccagttt
     7441 cttcttgaat tgttctcgaa tcggcctgtc cagtttgccc cactccttca aggccgattc
     7501 cttgaaggtg aggttataag gcatcaagag agaccggaac ggatttttcg cctcgtcgct
     7561 tgcggatggt ttttttaatt tccagatcgt ccagcttttc cagcatggcc tcgaaggtct
     7621 ccgccggcac aatataggcg gaaggccggt tgtgattgag aataaccacc ggcgccccct
     7681 ccgcttcctt gatgacggca gagggattct ttttcaggtc tgtgatgctg accgacgatt
     7741 ttgccaccag atagtccata gctcatcctc caataaaacc aatattcggt tctaaatata
     7801 tgtcatattg gccgagagaa caagcaatat cttgggtgtt tgctgaatgt aaaaagaaag
     7861 attgcgcttt tgccgaatgt catataacct gaattcccgg attattgtaa cgaggttggt
     7921 ggagtgatcc ccaatctgga
//
LOCUS       DAOSMF010000085         7872 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_10308_length_7872_cov_24.028016, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000085 DAOSMF010000000
VERSION     DAOSMF010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 7872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7872
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10308_length_7872_cov_24.028016"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="PLT63_08230"
     CDS             complement(<1..349)
                     /locus_tag="PLT63_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HOD98749.1"
                     /translation="MRGLYLVTDRDLCGGRPLEEVVAAAVAGGAACVQLREKHLSTRA
                     FVAEARAIKKLLADQGVPLIINDRLDVALAAEADGVHIGQDDMPYPLARRILGKGKII
                     GLSVETWEDVEEAE"
     gene            complement(354..1166)
                     /gene="thiM"
                     /locus_tag="PLT63_08235"
     CDS             complement(354..1166)
                     /gene="thiM"
                     /locus_tag="PLT63_08235"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HOD98750.1"
                     /translation="MTYRPEDVWEDLERIRTRRPLVHNITNYVVMNSTANALLAIGAS
                     PVMAHADQEVEDMVGIAAALVINIGTLSEQWTKSMFMAARRAGQLGIPIVLDPVGAGA
                     TPYRTRTARQLLEAIAPAVIRANASEIIAMVAADKRTKGVDSTAASSEALTAARTLNA
                     EFGSVVCVSGAEDYIVGGGRLFIVGNGHALMARVTGLGCTASALCGAFAAVRREDIIR
                     ATASAMAVMGIAGETAARADGPGSMQVMFLDALYGLTRRDIEERLKLTVQEL"
     gene            1412..>3396
                     /gene="carB"
                     /locus_tag="PLT63_08240"
     CDS             1412..>3396
                     /gene="carB"
                     /locus_tag="PLT63_08240"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOD98751.1"
                     /translation="MPKRSDINTVLIIGSGPIVIGQACEFDYSGTQACKALRKLGYRI
                     ILVNSNPATIMTDPGMADVTYIEPLNIPSLTEIIENERPDAILPNLGGQTGLNLTSEL
                     HKQGILDKYGVKVIGVRVDAIERGEDRIAFKEAMNRLGIEMPKSEPAYTVEEAEKIAA
                     ELGYPVVIRPAYTMGGTGGGLVYNLEELRTVSSRGISASMVGQILIEESVVGWEELEL
                     EVVRDAKNQMITVCFIENVDPMGVHTGDSYCTAPMLTISPELQARLQKYSYAIVEAIE
                     VIGGTNVQFAHDPKTGRVLVIEINPRTSRSSALASKATGFPIAFISSLLAAGLTLDEI
                     PYWREGTLDKYTPSGDYVVVKFARWDFEKFPGSLDRLGTQMRAVGEVMSIGKNYKEAF
                     LKAIRSLEKGRYGLGFANDLRAKSLDELMELLAEPSSERQYIMYEALRKGAEVETLHR
                     RTHIKPWFIEQMKALVELEERLLAYKGKILPDELLIQAKKDGFADRYLAQIMGGEETE
                     IRRRRLALGVAECWDAVPVSGVEDAAYYYSTYNAPDRVGVSANKKIMVLGGGPNRIGQ
                     GIEFDYCCVHAAFALRDEGYESIMVNCNPETVSTDYDTSNKLYFEPLTVEDVLAIYEK
                     EQPEGVIVQFGGQTPLNIAQELARAGVRIIGTSPETI"
     assembly_gap    3397..3496
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3497..4471
                     /locus_tag="PLT63_08245"
     CDS             <3497..4471
                     /locus_tag="PLT63_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HOD98752.1"
                     /translation="LIDKFLENAIEVEADAISDGSDAFVPAIMEHIELAGVHSGDSAC
                     VIPPISISARHMDTINDYTRRIAVEFGVIGLMNIQYAIADDIVYILEANPRASRTVPL
                     VSKVCNISMARIATQVMLGGKLADFKIKQRNIPHFGVKEAVFPFNMFQEVDPLLGPEM
                     RSTGEVLGLADSFGLAFFKAEEAAQQVLPGEGTVLLTVNDWDKRGVLEVARDFNELGF
                     RIMTTTGTNRFLAEEGIPSETLLKMHEGRPNIVDAIKNGLINLIINTPSGKLSQYDDS
                     YIRKAAIKYKIPYITTIAAAVAAVKGITAFRKGHGRAKSLQSYHADIR"
     gene            4526..>5685
                     /locus_tag="PLT63_08250"
     CDS             4526..>5685
                     /locus_tag="PLT63_08250"
                     /inference="COORDINATES: protein motif:HMM:NF024927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98753.1"
                     /translation="MSEIRDNCGIFGVYSKFPCVYDIYNGIDFLQHRGQEFCGIATFD
                     RHIHQVTHHGKVGNAFNEQDLLYLTGNWGIGHVSLWERQPMAWQSRLGNISVAFSGNV
                     INAEEIIAAMKDQGHSFYRSYNIEVIAKTIMEADDMVTGIAALTGRIKGAYSLVILAE
                     EGIYATRDIYGFRPLILGQNGTRFAVSSESRALQNLDMEVVRDVRPGEIILIDAKGFH
                     TLRQLPSPRKAHCAFEWAYTASIDSVIDGLYVQEARNRLGQALAQRDLDERDTSADIV
                     APVPMSGIGHALGYHQRSQIRYQEVFLYNRYADRSYTQSSQTAREKMAKRKLSLLKST
                     VKDKRIIICDDSIVRGTQIRDKVRDLKDSGAREVHVRIACPPLMYPCDFGVST"
     assembly_gap    5686..5785
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5786..6850
                     /gene="ribD"
                     /locus_tag="PLT63_08255"
     CDS             <5786..6850
                     /gene="ribD"
                     /locus_tag="PLT63_08255"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOD98754.1"
                     /translation="NPMVGAVIVKDGRIIGEGYHVCCGESHAEINAIRGARESIAGAT
                     FYVTLEPCTHQGRTPPCVQALIKEQPARVVIGAIDPNPVVAGRGVAALRAQGIETVVG
                     VREEECRRLNEKFFKFMTTGLPFVTLKYAQTLDGRIAGAGGHSRWISSLPARRYAHRL
                     RSVHDAVLVGTGTVRHDDPELTVRLVRGKNPLRIILDSRLDIPLKAGVFRNQDRAPTL
                     VVAAVNAPEDKLRALAGLGVETLLVAAAPGGINLPELFRKLGQRGITSVLVEGGGAII
                     TSVLRERLADRLAIVVAPKIIGRGVDAVGELGLTTMDQALQLTAVQWRRLGPDILCTG
                     DIAGNGYGPAKGESAGRGTE"
     gene            6904..>7872
                     /locus_tag="PLT63_08260"
     CDS             6904..>7872
                     /locus_tag="PLT63_08260"
                     /inference="COORDINATES: protein motif:HMM:NF040257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98755.1"
                     /translation="MQRHFTKKRKIRVIQYGLGYIGLETVRAILKKEALTIVGAVDTN
                     PRLAGKDLGELLKLPAPLGVTVSDQPRELLADVKADVVIHTTGSRIPSIYQQFEDILE
                     KKINIVSASEELLFPSAENAIMAERIHLLAMEKRVTVLGTGVNPGFVMDALPLFLTGV
                     CQEVREIHVQRVVDAATRRYALQKKIGTGMTVAEFQREIDKQALGHVGLRESLHLIID
                     KLSRPVDFVEESVAPVIAAHPIRTEYFEIKPGDVAGIEHLAKGIKDGKEILTLELRMY
                     VGAPDPHDAIQIVGTPDIKLRIEGGVAGDQATAAVLVNSIPAVLDAK"
BASE COUNT         1583 a         2492 c         2275 g         1322 t
ORIGIN      
        1 cctccgcctc ctccacgtcc tcccaggtct ccacggaaag accgatgatc tttcccttcc
       61 ccaggatgcg ccgggccagg ggatagggca tgtcatcctg gccgatatga acgccgtcgg
      121 cctccgcggc cagggctacg tctagacggt cattgatgat cagggggaca ccctggtccg
      181 ccagcagttt cttgatcgct cgggcctccg cgacgaaggc ccgggtggaa agatgcttct
      241 ccctgagctg cacgcaggcg gcgccgccag ccaccgccgc cgcgacgacc tcctccaggg
      301 gccgcccccc gcacagatcc cggtccgtaa ccagatacaa gccccgcatc gtcctaaagc
      361 tcctgaacgg tcagtttgag ccgttcctcg atatcccgac gcgtcaatcc atacagggcg
      421 tcgagaaaca tgacctgcat ggaacccgga ccgtcggcgc gggcggcggt ctccccggcg
      481 atccccatga cggccatggc ggatgccgtc gccctgatga tatcctccct cctcacggcg
      541 gcaaaggccc cgcacagggc tgaggcggtg cacccgaggc ccgtcacccg ggccatcagg
      601 gcatggccgt tgcccacgat gaacagccgc cccccgccga cgatgtaatc ctccgcaccg
      661 ctcacgcaga ccacggaacc gaattccgcg ttcagggttc gggcggccgt caaggcctcc
      721 gacgacgccg cggtgctgtc cacgcctttg gtccttttgt ccgccgccac catggctatg
      781 atctccgagg cgttggcccg gatgacggcg ggagcaatag cctccaggag ctgccgcgcc
      841 gtccgggtcc ggtagggggt ggccccggcg ccgacgggat cgagaacgat ggggatgccc
      901 agttgcccgg ccctgcgcgc cgccatgaac atggacttcg tccactgctc gctcaaggtg
      961 ccgatgttga tcaccagggc ggcagcgatt cccaccatat cctccacctc ctggtccgcg
     1021 tgggccatga cgggagaagc accgatggcc agcagggcgt tggcggtgga gttcatcacc
     1081 acatagttgg taatgttatg aaccagcggt ctgcgggttc tgatccgttc caggtcctcc
     1141 cagacatctt ccggccggta cgtcatttcc gtttctcctt tgcagaatga ttcacgccat
     1201 ctgacggcat caggctgggg gaagttgttc catatttccc gccgggagtc aaaaattttt
     1261 ttcgcgaatt caccttgaca ggaggcccgt acttccctat attccgcaag cccggcgcgg
     1321 tgttattatt gaagaaagct atataattgc gccacattgt atgaacggca atcggctccg
     1381 gggagccggc tgccaaacaa ggaggcgtcc catgccaaag agaagcgaca tcaacacggt
     1441 cctcatcatc ggttccggac ccatcgtcat cggccaggcc tgtgaattcg attattccgg
     1501 aacccaggcc tgcaaggccc tgcgtaagct cggttacagg atcatcctcg tcaattccaa
     1561 tcccgccacc atcatgaccg acccgggcat ggccgacgtc acctacatcg agcctctgaa
     1621 tatcccctcc ctcacggaaa tcatcgaaaa cgagcgtccc gacgccattc ttcccaatct
     1681 cggcggccag acgggtttaa acctcacctc ggagctgcac aagcaaggca tcctggataa
     1741 atacggcgtc aaggtcatcg gtgtccgcgt cgatgccatc gaacggggag aggaccggat
     1801 cgccttcaag gaagcgatga accgcctcgg gatcgagatg ccgaagagcg aaccggccta
     1861 taccgtcgag gaggcggaaa agatcgccgc ggaactgggc tatcccgtgg tcatccgtcc
     1921 cgcctacacc atggggggaa cggggggcgg cctggtttac aacctcgagg aactccgtac
     1981 tgtgtccagc cgcggcatct cggccagcat ggtgggccag atcctcatcg aggagtccgt
     2041 ggtcggctgg gaggagttgg agctggaagt ggtccgggac gccaagaatc agatgatcac
     2101 cgtctgcttc attgaaaatg tggaccccat gggcgtgcat accggggact cctactgcac
     2161 ggccccgatg ctgacgatca gtcccgaact ccaggcccgg ctccagaaat attcctacgc
     2221 catcgtggag gccatcgagg tcatcggcgg gaccaacgtc caattcgccc acgacccgaa
     2281 gacgggacgg gtattggtca tcgagatcaa cccccggacc tcccggtctt ccgccctggc
     2341 ctccaaggcc acgggcttcc ccatcgcctt catctcttcc ctcctcgctg cgggcctcac
     2401 cctggacgag atcccctact ggcgggaggg gaccctggat aagtacaccc cttcggggga
     2461 ttacgtcgtc gtcaagttcg cccgttggga tttcgagaaa ttccccggtt ccctggaccg
     2521 cctgggcacc cagatgcggg ccgtgggcga ggtgatgagc atcggcaaga attacaagga
     2581 ggccttccta aaggccatcc gctccctgga aaagggacgt tacggcctgg gtttcgcgaa
     2641 tgacctccgc gccaaatccc tcgatgaatt gatggaactt ctcgcggagc cctccagcga
     2701 gcgccagtac atcatgtatg aagccctcag gaagggggcg gaggtggaaa cacttcaccg
     2761 gcgcacccat atcaagccct ggttcatcga acagatgaaa gcactggtgg agttggaaga
     2821 gcggcttctg gcatacaaag gcaaaatact gcccgatgag ctgctcatac aggcgaaaaa
     2881 agacggcttc gccgaccggt atctcgccca gatcatggga ggcgaggaaa cggagatccg
     2941 gcgccgacgt ctcgccctgg gggtggcgga gtgctgggac gccgtgccgg tgagcggcgt
     3001 ggaggacgcc gcctactatt attccaccta taacgccccg gatcgggtgg gggtatccgc
     3061 caacaaaaag atcatggtct taggcggtgg tcccaaccgc atcggccagg ggatcgaatt
     3121 cgactactgc tgcgtccatg ccgccttcgc cctccgggac gaaggttatg aatccatcat
     3181 ggtcaactgc aaccccgaaa cggtctccac ggactatgac acctccaaca agctctactt
     3241 cgaacccctg accgtggagg acgtcctggc gatttacgaa aaggagcagc ccgaaggggt
     3301 aatcgtccag ttcggcggcc agacgcccct gaacatcgcc caggagctgg cccgggccgg
     3361 cgtcaggatc atcggcacct ccccggagac catcgannnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnctca tcgacaagtt tctggaaaac gccatcgagg tggaggccga
     3541 cgccatctcc gacggttcgg acgccttcgt tcccgccatc atggaacaca tcgaactggc
     3601 gggggtccat tccggcgact ccgcctgcgt gatcccaccc atcagcatct ccgcccgcca
     3661 catggacacc atcaacgact acacccggcg gatcgccgtg gagttcggtg tcatcggcct
     3721 gatgaacatc cagtacgcca tcgccgacga catcgtctat atattggagg ccaatccccg
     3781 ggcctcccga accgtgcccc tggtttccaa ggtctgtaac atctccatgg cccgcatcgc
     3841 cacccaggtc atgctggggg gaaagctggc cgatttcaaa atcaaacagc gtaacattcc
     3901 ccacttcggc gtcaaggagg ccgtcttccc cttcaacatg ttccaagaag tcgaccccct
     3961 gctggggccg gaaatgcgct ccaccggcga ggtgctggga ctggccgatt ctttcggcct
     4021 ggccttcttc aaggcggagg aagcggcgca gcaggttctg cccggagagg gaacggtgct
     4081 gctcaccgtc aacgactggg acaagcgggg ggtcctggaa gtcgcccggg atttcaacga
     4141 actgggtttc cggatcatga ccaccaccgg gaccaaccgt ttcctcgcgg aggagggaat
     4201 tccttcggaa acgctcctga agatgcacga gggacggccc aatatcgtgg acgccatcaa
     4261 gaacgggctc atcaacctga tcatcaacac ccccagcggc aagctgagcc agtatgacga
     4321 ttcctacatc cgcaaggcgg ccatcaagta caaaatcccc tacatcacca ccatcgccgc
     4381 ggccgtggcg gcggtaaagg gaataacggc attccggaag gggcacggcc gggcgaagtc
     4441 cctgcaaagc taccatgccg atatcaggta gaaccagaca acaggtaaca gaccagagaa
     4501 gaccagacga caggcgacag gcaagatgag tgaaatcaga gacaattgcg gaatattcgg
     4561 cgtttattca aagtttccct gtgtttatga catctacaac gggatagatt ttctccagca
     4621 ccgcggccag gaattctgcg ggatcgccac tttcgaccgg cacatccacc aggtgactca
     4681 ccacggcaag gtgggaaacg ccttcaacga acaggatctt ctctacctca ccggtaactg
     4741 ggggatcggc catgtaagcc tctgggaacg ccagcccatg gcctggcagt cccgcctcgg
     4801 caacatctcc gtggccttca gcggcaatgt catcaacgcc gaggagataa tcgccgccat
     4861 gaaggaccag ggccattcct tctatagaag ctacaatatc gaagtgatcg ccaagacgat
     4921 catggaggcc gacgacatgg tgaccgggat cgccgccctg accgggcgca tcaaaggcgc
     4981 ctattccctg gtgatcctcg ccgaggaagg gatctacgcc acccgggaca tctatggctt
     5041 ccgccccctc atcctcggtc agaacgggac ccgcttcgcc gtcagctccg aatcccgggc
     5101 cctccagaac ctcgacatgg aggtcgtccg cgatgtccgt ccgggggaaa tcatcctcat
     5161 cgacgccaag ggattccaca ccctccggca actgccatcg ccccgaaagg cccattgcgc
     5221 cttcgaatgg gcctatacgg ccagcatcga ctccgtcatc gacggcctgt acgtccagga
     5281 ggcgagaaac cgcctcggac aggccctcgc ccagcgggac ctggacgaac gggacacgtc
     5341 cgcggacatc gtggccccgg tgcccatgtc cggcatcggc catgccctgg gctaccacca
     5401 gcgctcccag atccgctatc aggaggtgtt tctctacaac cgctatgccg accgcagcta
     5461 tacccagtcc agccagacgg cccgggaaaa gatggccaaa cggaaacttt ctctcctcaa
     5521 atcgaccgtg aaggacaagc ggatcatcat ctgcgacgat tccatcgtcc ggggcaccca
     5581 gatccgtgac aaggtgcggg acctgaaaga ctccggcgcc agagaggtcc atgtccggat
     5641 cgcctgcccg cccctcatgt acccctgcga cttcggcgtc tccacnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnaatcc catggtcggc gccgtcatcg tcaaggacgg
     5821 ccggatcatc ggggaaggct atcacgtctg ctgcggcgag agccacgccg agatcaacgc
     5881 catccgcggg gcccgggagt ccatcgccgg cgccaccttc tacgtcaccc tggagccctg
     5941 cacccaccag gggcggacgc ccccctgcgt ccaggccttg atcaaggagc agccggcccg
     6001 ggtggtgatc ggtgccatcg atccgaatcc cgtcgtcgcc ggccgggggg tggcggccct
     6061 gcgggcccag ggcatcgaaa ccgtcgtcgg cgtccgggag gaggaatgcc gccgcctgaa
     6121 cgagaagttt ttcaagttca tgaccaccgg tcttcccttc gtcaccctga aatacgccca
     6181 gaccctcgat ggccgcatcg ccggcgccgg aggtcattcc cggtggatca gctcactgcc
     6241 ggcccggcgc tacgcccacc gcctgcggag tgtccacgac gccgtgctcg tcggcaccgg
     6301 cacggtgcgc catgacgatc ccgaactgac ggtccgcctg gtccggggga aaaatcccct
     6361 gcggatcatc ctggacagcc gactggacat cccgcttaaa gccggcgtct tccggaacca
     6421 ggaccgggcg ccgaccctgg tcgtcgccgc cgtgaacgcc ccggaagaca aattacgggc
     6481 cctggccggg ctgggcgtgg aaacgcttct ggtcgccgcc gcaccggggg gaatcaacct
     6541 gccggagctg ttccggaaac tgggtcagcg gggcataacc tccgtcctgg tggagggggg
     6601 cggcgccatc atcacctcgg tgctccggga gaggctggcg gaccgactcg ccatagtcgt
     6661 ggcgcccaag atcatcggcc ggggcgtgga tgccgtgggt gagttgggcc tcaccaccat
     6721 ggatcaggcc ttgcaactta cggcggtgca atggcgtcgc ctcggtccgg atattctctg
     6781 caccggggat atcgccggca acggctacgg gccggctaaa ggcgaatcgg cgggccgggg
     6841 gacggaataa aacagcgggg tcgaatcgga ttcgacggca aaacgaacaa ggtagggctg
     6901 gctatgcaga gacattttac aaaaaagaga aagattcggg tcattcagta cggtttggga
     6961 tacatcggcc tggaaacggt ccgggccatt ctcaaaaagg aggcgctgac catcgtcggc
     7021 gccgtggata ccaacccgcg cctggcggga aaggatctgg gggaattgct gaagctgccg
     7081 gcgccgctgg gggtaacggt cagtgaccag ccccgggaac tcctggccga cgtcaaggcc
     7141 gacgtggtga tccacaccac cggttcgagg atcccgtcca tctaccagca gttcgaggat
     7201 atcctcgaaa aaaagatcaa catcgtctcc gccagtgaag aactgctctt cccttcggcg
     7261 gaaaacgcga tcatggcgga acggatccat ctgctggcga tggaaaaacg ggtcaccgtc
     7321 ctggggacgg gggtcaatcc cggcttcgtc atggacgccc tgccgctgtt tctcaccggc
     7381 gtctgccagg aagtgcggga aatccacgtc cagcgcgttg tggacgccgc cacccggcgc
     7441 tacgccctgc agaagaagat cggcacgggc atgaccgtgg cggagttcca gcgggaaata
     7501 gacaaacagg ccctggggca tgtggggctc cgggaatccc tccatctgat catcgacaaa
     7561 ctcagtcggc ccgtggactt cgtggaggag tccgtggccc cggtaatcgc cgcccatccc
     7621 atccggacgg aatatttcga gatcaaaccg ggagacgtgg cggggatcga acatctcgcc
     7681 aagggcatca aagacgggaa agagatactg accctggagc tccggatgta tgtcggcgcc
     7741 cccgaccccc acgacgccat tcagatcgtg ggcactccgg atatcaagct gaggatcgag
     7801 ggtggcgtcg ccggcgacca ggccacggcg gccgttctgg tcaactccat cccggcggtg
     7861 ctggacgcca ag
//
LOCUS       DAOSMF010000086         7744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_10515_length_7744_cov_23.812459, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000086 DAOSMF010000000
VERSION     DAOSMF010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 7744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7744
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10515_length_7744_cov_23.812459"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="PLT63_08265"
     CDS             complement(<1..218)
                     /locus_tag="PLT63_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase-like
                     domain-containing protein"
                     /protein_id="HOD98756.1"
                     /translation="MLERIRIFSGNATPDLAARISKNLGVPLGKANVSIFSDGETRVE
                     INENVRGMDVFIIQSTCPPVNVTLMELL"
     gene            complement(252..326)
                     /locus_tag="PLT63_08270"
     tRNA            complement(252..326)
                     /locus_tag="PLT63_08270"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(292..294),aa:Gln,seq:ttg)
     gene            complement(401..1261)
                     /gene="ispE"
                     /locus_tag="PLT63_08275"
     CDS             complement(401..1261)
                     /gene="ispE"
                     /locus_tag="PLT63_08275"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOD98757.1"
                     /translation="MTFEMNNPVRCMSPAKVNLYLKVLSRRPDGYHDLATLMQRISLY
                     DEMFFRLTSAAGIVLHCPGSSLPEDKANIVHRAAESFFHRVGFSGGVEIILKKSIPLA
                     AGLGGGSSNAATTLLTLNEFAGRPCSRTDLMEIGVALGSDIPFFILEATAWAFGIGDR
                     LEAVAHPPQFHFLLVNPGFELSTRKVYEGLNLTLTKEPIHYSIPRFFECHDVIKGLHN
                     DLETVSINLYPVLDEIKALLLSHGAAGALMSGSGPTIFGVFDNEDMAMKAEESLQGYG
                     TWSLFRAHTI"
     gene            complement(1382..1804)
                     /locus_tag="PLT63_08280"
     CDS             complement(1382..1804)
                     /locus_tag="PLT63_08280"
                     /inference="COORDINATES: protein motif:HMM:NF020473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HOD98758.1"
                     /translation="MEEGKQGKGFVIKDRRLFDETGEVRREDDMGQPPTEESRPQSTS
                     DAPAREEEELFLPEVNFMNFILSLSTTAMFHFGDFPDPVSGKSKKNMAAAKHTIDTIT
                     MLAAKTKGNLDDNEKNVLDAVLFELRMRYVKECGAPAS"
     gene            complement(1937..4543)
                     /gene="clpB"
                     /locus_tag="PLT63_08285"
     CDS             complement(1937..4543)
                     /gene="clpB"
                     /locus_tag="PLT63_08285"
                     /inference="COORDINATES: protein motif:HMM:TIGR03346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HOD98759.1"
                     /translation="MRFDKMTLKVQEAVQESQALANRYGHQTIEVEHLLLALTNQPDG
                     IVTAILKKLGADPGALAAVLQKSLEKLPRVEGSGSTPPYLSARLNKIIDQAMTEAARL
                     KDEYISAEHLLVAMSEEKAGAAAEILRQTGISKDSIFKILVDIRGKQRVTDPNPEDKY
                     QALKRYAKDFNEIARKGGFDPVIGRDEEIRRIMQVLSRRTKNNPVLIGEPGVGKTAIV
                     EGLAQRIINGDVPENLKNKRVIGLDIGALVAGAKYRGEFEERLKAVLREVIDAEGEII
                     LFIDEIHTVVGAGASEGSMDASNMLKPALARGELRCVGATTLNEYRKHIEKDPALERR
                     FQPILVREPTVEDTIAILRGLKERYEIHHGVRIKDAAIVAAATLSHRYISDRFLPDKA
                     VDLIDESASRLRIELDSMPAEIDAIDRKIIQLEIERQSLKKETDASSQERREKIEREL
                     SELRESMDQMKLHWSSEKEAIKSIQTIKGRIEALKNEEMKAQREGNLARAAEIRYGKL
                     VELHQEMEHKQERLAAAQEDKQMLKEEVDAEDVAEVVANWTGIPLSRMMESDVEKLIH
                     MEERLKLRIIGQDEGIAAVSSALRRARSGLQDPNRPIGSFIFLGPTGVGKTELARALA
                     EFMFDNEQAMIRIDMSEYMEKHSVARLIGAPPGYVGYDEGGYLTEAVRRRPYAVLLFD
                     EIEKAHQDVFNILLQILDDGRLTDGHGRTVDFKNTVIIMTSNVGSQWLQDGELADEEK
                     RARSTDALRATFKPEFLNRIDDIITFRSLNLSDIERIVEIQIGIIQKRLAERKLTLEL
                     TDRAKDYIARNSYSSVYGARPLKRTLQKLVLDPLALKLLSGAFTEGDRITADLDTHDE
                     LQFARKEVKKKK"
     gene            complement(4557..5423)
                     /locus_tag="PLT63_08290"
     CDS             complement(4557..5423)
                     /locus_tag="PLT63_08290"
                     /inference="COORDINATES: protein motif:HMM:NF014510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HOD98760.1"
                     /translation="MKPIHNVMLEFGCRIAQEIEARALLLYADAAFELPLYEEGKRGF
                     DLILVTKDNHVPERFAGSSLSLNVPNVNLTRVGQIKIAVTKGIALGLFKKGDKIVCLS
                     GVPKFGYVDSIFVIDVGKEFEILTSEYLTDLTEKVYPEVFGAILNLSIELAAQGREGR
                     KVGTTFVIGDHDRVLQLSRQMIINPFMGYSENERNILNPELKETIKEFSAIDGAFVIR
                     EDGTIITAGRHLNAALESKDFPHGLGSRHIAAAGITSITNATAIVLSESTGNVSIFKN
                     GKIFVSIEKPIE"
     gene            5576..6433
                     /gene="rpoH"
                     /locus_tag="PLT63_08295"
     CDS             5576..6433
                     /gene="rpoH"
                     /locus_tag="PLT63_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HOD98761.1"
                     /translation="MDVPALTGTLDRYIAEINQFPLLTPEEEFSLAVRLKKFNDMEAA
                     EKLVVSNLRFVVKIAHEYRNYGIKLADLIQEGNIGLMHAVKKFDPYKGYRLISYAVWW
                     IRAYMQNYIIKTWSLVKIGTTQAQRKLFFKLGQAKRKLEVLSQKNPEFGEIAASLGVK
                     SIEIEEMDLRMSNRDVSLDAFINDDSDATHIDYLTYDGEDQETALIRKEEMDILKSHI
                     AGALNKLTEKEKYIIKKRVMADDPMTLQEIGDRYKITRERARQIEKQALKKLRLSIPY
                     LGETPPLLE"
     gene            complement(6443..>7744)
                     /gene="dnaB"
                     /locus_tag="PLT63_08300"
     CDS             complement(6443..>7744)
                     /gene="dnaB"
                     /locus_tag="PLT63_08300"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOD98762.1"
                     /translation="EAEQSVLGGILLDNQGLNAVLEVITGRDFYSEAHRRIFEAMINL
                     SERGEPVDLITLSSILKDRTQLDKAGGAAYLAGLVDNVPSAANIVHYAKIVKEKAILR
                     GLINTATEILGKTYAPGADVDTVLDEAEQAIFEISEKKIRPAFFPIKDIVKDSFRTIE
                     ELYARKELITGVPTGFEKVDDLTSGLQNADLIIIAGRPSMGKTAFALNIAQYVGIHRA
                     LPVCLFSLEMAKEQIAMRMLAATAKVDSQRIRKGFLGETDWPKLTTAAGILSEAPIFI
                     DDTAAITVLEMKAKARRLKAEHGLGLVIIDYLQLMRSGTAKDSREQEISEISRSLKAL
                     AKELSVPVIALSQLNRKVEDRTNRRPQMADLRESGAIEQDADVIAFIYRDEVYNKAED
                     NPEKGMAEVIIAKQRNGPIGTVKLAFLEKFTSFENLARRGD"
BASE COUNT         1587 a         2067 c         2161 g         1929 t
ORIGIN      
        1 atcaggagct ccatgagggt gacattcacg ggcgggcacg tcgattggat gataaagaca
       61 tccatccccc gcacattttc gttgatttct acccttgttt ctccgtcact gaagatcgat
      121 acattggcct tgcccagggg gacgcccaag ttcttgctta ttctagccgc tagatccggt
      181 gttgcgtttc cggagaagat acgaattcgt tccagcatct gattcctctt ctgcaacgtg
      241 ctgcagcgta ttggctgggg cgggaggatt cgaacctccg tatgcaagat ccaaattctt
      301 gtgtcttacc gcttgacgac gccccattgt tgtccgtggt taacgaggaa cggctcgggt
      361 gtgaagaaac aaagagagag gattgtgccg aaacgtttat tcaaatggtg tgggcacgaa
      421 aaagagacca agtcccgtat ccctgcaatg attcctccgc cttcatcgcc atatcttcgt
      481 tgtcaaagac accgaaaatc gtgggaccgc ttccagacat cagcgcgcct gcggcgccat
      541 gactgaggag aagcgccttg atttcatcca agacggggta aagattgatg gacacagtct
      601 ccaagtcatt atgcagtccc ttgatcacat catggcattc aaaaaatcga gggatgctat
      661 aatggattgg ctcttttgtc aatgttaaat tcaaaccttc ataaaccttc cgggtggaaa
      721 gttcgaaacc gggatttacc agcaggaagt ggaactgtgg cggatgggca acggcttcca
      781 gtcggtcgcc gattccgaac gcccaggccg ttgcttccag gatgaagaat gggatgtctg
      841 acccgagggc gacgcctatt tccatcaggt cggttcgcga gcagggacgg ccggcaaatt
      901 cattgagggt gagcagggtc gtggcagcgt tggaactgcc tcctcccagt ccggcggcca
      961 ggggtatgga tttcttgagg atgatctcca ccccgccgga aaagccgacg cggtgaaaga
     1021 aactctcggc ggcgcgatgg acgatgttcg ctttgtcttc gggcagactc gatcccgggc
     1081 aatggaggac gattccggct gccgaggtga gccggaaaaa catctcatca tagagactga
     1141 tccgctgcat gagcgtggcc aggtcatggt atccgtccgg acgacgggaa aggactttca
     1201 gatacaggtt taccttggcc ggagacatgc atcgcaccgg gttgttcatc tcaaaggtca
     1261 tgatttggca agattcggat tcgccgtggt ttttcgtatc ggagaacata tcgcgaccct
     1321 ggtaaacttg gttcacaaac ccgggcggca acgttcttac tgcacaagct ggaggtgacc
     1381 ttcacgatgc tggtgccccg cattccttga catagcgcat gcgcagttcg aaaagaacag
     1441 catcgagaac gtttttctcg ttatcgtcca gatttccctt tgttttagcg gccagcatcg
     1501 taatggtatc gatggtgtgc ttggcggccg ccatattttt tttggacttg cctgagacgg
     1561 gatcgggaaa atcaccgaaa tgaaacatag ccgtcgtact taaggacaga atgaaattca
     1621 tgaaattgac ctcgggaagg aatagttcct cttcttcccg ggccggtgca tcgctggtgg
     1681 attgggggcg gctttcctcc gttgggggtt gccccatgtc atcttcccgg cgaacctcac
     1741 cggtttcatc gaacaggcgg cgatccttga ttacaaatcc ttttccctgt tttccctctt
     1801 ccatatacca ctcctttttg catttgcact ggttcccatt ttaccgcctg cctcttccgt
     1861 ccggaacgcc cggcggatcc tgaatcgatt ctccggtact cttagggaaa gggggtgacg
     1921 gttgggatct gcctgtctat ttcttctttt tgacctcttt cctcgcaaac tgtaattcat
     1981 cgtgggtgtc gagatcggcg gtgattctgt ctccttcagt gaaagccccg gagaggagct
     2041 tgagcgccag gggatctagg acaagctttt gcaacgtccg ttttaggggt cgggcgccgt
     2101 aaacggagct gtagctgttg cgggctatat aatcttttgc ccggtctgtc aattccaggg
     2161 taagcttgcg ctcggccaga cgcttctgga taataccgat ttgaatttca acgatccgtt
     2221 cgatatcact caagttcaat gagcggaagg tgataatgtc atcgatccgg ttgagaaatt
     2281 ctggtttgaa ggtagcgcgg agggcgtcgg tgctacgggc ccgtttttcc tcatcggcaa
     2341 gttcgccatc ctgaagccat tggctgccga cgttggaggt catgatgatt accgtatttt
     2401 tgaagtccac ggttctcccg tgaccatccg tcaaccgccc gtcatcgagg atttggagga
     2461 ggatgttgaa cacatcctga tgggcctttt caatctcatc gaaaaggagg accgcgtatg
     2521 gccgtcgccg gactgcttca gtgagataac ccccttcgtc gtaacctacg tatccgggcg
     2581 gggcgccgat caaacgggct acagagtgtt tttccatgta ttcggacatg tcgatgcgga
     2641 tcatggcctg ttcgttatca aacatgaact ccgctagggc ccgggccagt tccgtctttc
     2701 ccacacccgt ggggccgagg aagataaagg agccgatggg acggttgggg tcctggagcc
     2761 cggaacgggc gcggcgaagg gcgctggaca cggcggctat gccttcgtcc tgaccgatga
     2821 tccgcagctt gagccgctct tccatgtgga tcagcttttc gacatcgctt tccatcatcc
     2881 gggacagtgg aattccagtc cagtttgcca cgacctcggc gacatcttcg gcgtccacct
     2941 cttctttcag catctgcttg tcctcctgag cggcggcaag gcgctcctgc ttatgttcca
     3001 tctcctggtg gagttccacg agtttcccat aacggatttc cgccgcgcgg gcgagatttc
     3061 cctcccgctg ggccttcatc tcctcgtttt tcagggcctc aatccggccc ttgatggtct
     3121 ggatgctctt gatcgcctct ttttcgctgc tccagtgcag tttcatctga tccatggatt
     3181 ctctgagttc cgacagttcc ctttcgatct tctcccgtcg ctcctgggaa gaggcgtcgg
     3241 tttccttttt gagggattgc cgttcgatct ccagttggat gatcttccgg tcgatggcgt
     3301 caatctccgc tggcatgctg tccagttcga tccgcaaccg ggaagctgac tcgtcgatga
     3361 ggtccacggc cttgtcagga agaaaacggt cgctgatata acggtgggag agggtggcgg
     3421 cggcgacgat ggccgcgtcc ttgatccgga cgccgtgatg aatctcgtag cgttctttga
     3481 gcccccggag gatggcgatc gtgtcctcca cggtcggctc acggacgagt atgggctgaa
     3541 aacgcctctc caacgccggg tccttttcga tatgtttccg gtattcattg agggtggtgg
     3601 cgccgacgca acgcaactcc ccccgggcca gggccggctt gagcatgttc gaggcatcca
     3661 tggaaccttc ggaggccccg gcgcccacca cggtgtggat ttcatcgatg aagaggatga
     3721 tttccccttc cgcgtcgatt acctccctga ggacggcctt gagacgttcc tcgaattctc
     3781 cccgatattt ggccccggcg acgagggcgc caatatcgag gccgatgacc cgtttatttt
     3841 tcagattttc gggtacgtcc ccgttgatga tccgctgggc cagcccctcg acgatggccg
     3901 tttttcccac cccgggctcg ccgattaaaa cggggttgtt cttggtccgc cgggagagga
     3961 cttgcatgat ccttcggatt tcctcgtccc tgccgatgac aggatcgaag ccaccttttc
     4021 tcgctatttc attgaaatct ttggcgtagc gtttgagggc ctggtattta tcctccggat
     4081 tcgggtccgt tacccgctgt ttgccccgga tgtccacgag gatcttgaag atactgtcct
     4141 tgctgatgcc ggtttgccga agaatttccg ccgccgcccc tgccttctct tcggacatgg
     4201 cgacgaggag atgctccgcg ctgatgtatt catctttcag gcgggcggct tcggtcatcg
     4261 cctgatcaat gatcttgttc aaacgggcac tcagataggg cggtgtcgat cctgaacctt
     4321 ccaccctggg cagtttttcc agtgatttct gcagaaccgc cgccaacgcg ccggggtccg
     4381 ccccgagctt cttgaggatc gccgtgacga tcccatcggg ctggttagtt agggccagca
     4441 gcaggtgctc gacctcgatt gtctggtgac cgtatctatt ggccagggct tgggattcct
     4501 gaacagcttc ctggaccttg agggtcattt tatcaaatct cataatattt cctcacttat
     4561 tcaataggtt tttcgatact cacgaagatc ttgccgttct tgaaaatact gacgttgccg
     4621 gtggattcgg aaaggacgat cgccgtggcg ttggtgatgc tggttattcc ggctgcggcg
     4681 atatggcggc ttcccagccc gtggggaaag tctttgctct ccagtgcggc attgagatgc
     4741 cgccccgcgg tgatgatcgt gccgtcttcc ctgatgacga aggccccgtc aatggcggag
     4801 aattccttga tcgtttcctt cagctcggga ttcaggatgt tacgttcgtt ttccgagtag
     4861 cccataaagg gattgatgat catttgacgg gagagctgca ggacccgatc gtggtcgccg
     4921 atgacaaagg tggttcccac tttccttcct tcccgcccct gggcggccag ttcgatggac
     4981 aggttcagga tcgctccgaa cacctcagga taaacctttt ccgtcaggtc tgtcaaatat
     5041 tccgaggtca ggatttcaaa ttccttgccg acatcgatga cgaagatact gtccacgtaa
     5101 ccgaatttgg gaaccccgct caagcagact attttgtccc cctttttgaa aaggcccagg
     5161 gcgatgccct tggtgacggc aattttgatc tgtcctaccc gggtcagatt gacattgggg
     5221 acgttaaggc tcaacgagga tccggcgaac ctttccggga catggttgtc tttagttacg
     5281 agaatgagat cgaagccgcg cttcccctcc tcgtagagcg gcagttcgaa ggcggcgtcg
     5341 gcgtacagga gcagggcccg ggcctcgatt tcctgggcga tgcggcatcc gaattccagc
     5401 attacattgt gtatgggttt catgtttttt tcatcccttt tgatggttat ctaaattaat
     5461 tatgctttcc tccctgtcaa gacgacgccc ggggtgaaaa atgccttgac agcggcggta
     5521 aagaagcata tatatcctaa cctttcgaaa ttgtttgcat atgaggtaag accacttgga
     5581 tgttcccgca ttaaccggca cgcttgaccg ttatatcgct gaaataaatc agttccccct
     5641 gctaactccg gaagaggaat tcagtctggc ggtgcggctg aaaaaattca acgacatgga
     5701 ggcggcggaa aaacttgtcg tctcgaacct gcgctttgtc gtcaagatcg cccatgaata
     5761 ccgtaattac ggtatcaagc tcgcagatct gatccaggag ggcaacatcg gactgatgca
     5821 tgccgtcaag aaattcgacc cctataaagg ctatcgtctg atctcctatg cggtctggtg
     5881 gatccgggcc tatatgcaga actatatcat caagacctgg agcctggtaa aaataggcac
     5941 cacccaggcg cagcgcaaac tgtttttcaa gctcgggcag gcgaaaagga aactcgaggt
     6001 gctgtcgcag aagaatcccg agttcgggga aatcgccgcc tcgctggggg taaaatccat
     6061 cgagatcgag gagatggatt taaggatgag caatcgcgac gtttcccttg atgcgttcat
     6121 aaatgacgac agcgatgcca ctcatatcga ctacctgacc tacgacggcg aagatcagga
     6181 gacggcgctg atcaggaaag aggagatgga tatcctcaag agtcatattg ccggcgcttt
     6241 gaacaaattg acggagaagg aaaaatacat catcaaaaaa cgcgtcatgg ccgatgaccc
     6301 catgacgctc caggagatcg gcgaccgcta caagattacc cgggagcggg caaggcagat
     6361 agaaaagcag gccctgaaaa aactgcgtct ctccattcct tatctagggg agaccccccc
     6421 cctactcgaa taaaccaaat cctcagtcgc cgcggcgagc gagattttca aaggaagtaa
     6481 atttttccag aaatgccagt ttgacggtgc cgatggggcc gttgcgctgc ttggcgatga
     6541 tgacttccgc catgcccttt tcgggattgt cctcagcctt gttgtatacc tcgtcccgat
     6601 agatgaaggc gataacgtcg gcatcctgct cgatggcacc cgattcccgg agatccgcca
     6661 tctgggggcg gcggtttgtg cggtcctcga ccttgcggtt gagctgggaa agggcaatga
     6721 cggggacgct taactctttg gccagggcct tcaaagagcg gctgatctcg gagatttcct
     6781 gctcccggga atctttagcc gtaccgctac gcatcaactg gagataatcg atgataacca
     6841 gtcccaatcc gtgctccgcc ttcagacgcc gagccttggc tttcatttcc aaaacggtga
     6901 tcgcggcggt gtcgtcgatg aagatcggcg cctccgagag gatgccagcg gcggtggtga
     6961 gtttcggcca gtctgtttcc cctaaaaaac ctttgcggat acgctgcgaa tcaactttgg
     7021 cggtggcggc gagcatccgc atggcgattt gttccttggc catttccagg gagaacaggc
     7081 ataccgggag agctcggtgg atgccgacat attgggcgat gttgagggcg aaggccgtct
     7141 tgcccatgct aggtctaccg gcgattatga tcaggtcggc gttctgcaag ccggaggtga
     7201 gatcgtcaac cttttcgaag cccgtaggca cgccggtaat aagttctttt cgggcgtaga
     7261 gttcttcgat ggtccggaag ctgtccttga cgatgtcctt gatggggaag aaggcagggc
     7321 ggatcttctt ctccgagatt tcgaagatgg cctgttccgc ctcatcgagg accgtatcca
     7381 catccgcgcc gggggcgtag gtcttgccga ggatctctgt ggcggtattg atcaggccgc
     7441 gcaagatagc tttttctttg actatcttag cgtagtgaac gatattggcc gccgacggca
     7501 cgttgtccac cagaccggcg agataggccg caccgcctgc cttgtcgagt tgtgtccggt
     7561 ccttgaggat gctgctcaag gtaatgaggt ccaccggttc gccgcgttcg gatagattga
     7621 tcatcgcttc gaagatccgg cggtgggcct cgctgtaaaa atcacggccg gtaatgacct
     7681 cgagaacggc gttcaatccc tggttgtcaa ggagtatccc tcccagaacg gattgttccg
     7741 cctc
//
LOCUS       DAOSMF010000087         7485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_10970_length_7485_cov_24.550471, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000087 DAOSMF010000000
VERSION     DAOSMF010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 7485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7485
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10970_length_7485_cov_24.550471"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1341
                     /locus_tag="PLT63_08305"
     CDS             <1..1341
                     /locus_tag="PLT63_08305"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF006396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HOD98763.1"
                     /translation="PIIAQLACMEVGKTRDGHSVTEAFRILTDAGADIIGLNCRLGPA
                     ELLRTLEQSVVLPDTLLSVFPNAGRLGIMEGEFGYTSSPAYFADRARHFHAQGARIIG
                     GCCGTTPDHIRAVSAVLKDLRPLARINPEAPPEPAPRPAVISSGGTGTITDKVKEWPT
                     VLVEFDPPKDLDIERYMIGAASLQDAGADSITMADNSLAQTRMSNLALGAIVKMRLGI
                     DPLVHIACRDRNLIGQQSHLMGLHALSIHQILVVTGDPARYGDLPGASSVFDVSSFDL
                     IRMVKQLNAGISFSGQPLRERGRFVVGAAFNPHVTNLTGAFNRLGKKIEAGADFILTQ
                     PVYDRGTIEMIAERTRHLPVPIFIGIMPITSARNAQFLHNEVPGIRLTDTVLKRMGKY
                     PAAGPDARREGLDLARELVDAARECFRGFYLMTPFSYYELTATLTRHVKDGGGG"
     gene            1496..2227
                     /locus_tag="PLT63_08310"
     CDS             1496..2227
                     /locus_tag="PLT63_08310"
                     /inference="COORDINATES: protein motif:HMM:NF015961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOD98764.1"
                     /translation="MAKRDKYTFSFWEKLLLHLAPPIAAGAIKLWNRTCRVVVRENEE
                     RELAALHENGGCIYATWHQRMFWFFHDFGSRHVTMMISRSKDGEYANAVALRLGFYSV
                     RGSNSLKGREAMYYLVELLKERAGHKAGMMADGPNGPPRQLKMGTIKIARETGLPIIP
                     MMYGAKRRIVFQSWDRYFLPCPFTDIVVLHGEPVFVPVDADEEECERLRRLVTDRMNE
                     MADRCDTWWGGIPVGKPGFDLPASS"
     gene            complement(2356..2724)
                     /locus_tag="PLT63_08315"
     CDS             complement(2356..2724)
                     /locus_tag="PLT63_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOD98765.1"
                     /translation="MTRDRISAGRRGEELAADCLARVGYTILARNWRCPLGELDIVAR
                     DGETLVFVEVRTRRSARFGSPEESVGFSKRQKLSRLAAFYLHRHGLFDVPARFDVVAV
                     HLLPSGPELELIRDAFDLIS"
     gene            complement(2721..3341)
                     /locus_tag="PLT63_08320"
     CDS             complement(2721..3341)
                     /locus_tag="PLT63_08320"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOD98766.1"
                     /translation="MNYFEERARQRGFRRIAGIDEAGRGPLAGPVVAAAVVLPDRYSH
                     PDINDSKKLTSRSRELLFEVIRTDALAVGIGVVDSRVIDELNILRATLLAMREAVADL
                     QVVPDCLLVDGNQSVPLEIPQETIVKGDGRSVSIAAASIVAKVSRDRIMEIYHRQFPY
                     YNFFKNKGYGTAEHRRAIREHGFCKIHRRSFHIKDTTQDNYELIFQ"
     gene            complement(3358..3705)
                     /gene="rplS"
                     /locus_tag="PLT63_08325"
     CDS             complement(3358..3705)
                     /gene="rplS"
                     /locus_tag="PLT63_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOD98767.1"
                     /translation="MNVVELIEKEQIRTDLPAFKPGDSIKVFVRIVEGQKSRIQAFEG
                     VVIRKRRGSCSSSFTVRKISYGIGVERTFPLHSPIIDRIEVVTRGRVRRSRLYYLRGL
                     KGKKARIKEEGKS"
     gene            complement(3864..4451)
                     /locus_tag="PLT63_08330"
     CDS             complement(3864..4451)
                     /locus_tag="PLT63_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOD98768.1"
                     /translation="MSRGIIVAAQPECYLALLHYPVLNKHRQVVTTAVANLDIHDIAR
                     AARTYGIAHYYIVTPIAAQRSLVETIINHWRRGYGAEYNHSRREAFSRVCVREDFEQV
                     CRDIGERAGKKPRVVATGAALEKGLISYGELRKALVEGDTPWLLVFGTGWGLADSIVA
                     RADCSLPPVRGGSDGYNHLSVRSAVAVVLDRLFGC"
     gene            complement(4528..5292)
                     /gene="trmD"
                     /locus_tag="PLT63_08335"
     CDS             complement(4528..5292)
                     /gene="trmD"
                     /locus_tag="PLT63_08335"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOD98769.1"
                     /translation="MRFDILTLFPEMFVSPFAGSILGKARERGLIDLQVHDIRSYAPG
                     KHRVTDDAPYGGGGGMVMKVEPLALALEALAMPSEAGPVILMSPQGAPFRQEIATELA
                     VCERILLICGHYEGVDERVREHLVNRELSIGDYVLTGGELAAMVVVDAVARLIPGVLG
                     NSESARADSFAAGLLEYPQYTRPQKYRGWEVPEVLVNGNHREIENWRRRQSLLRTWRR
                     RPELLAGAELSEADRTFLRELEAAEKEDRRETRPGR"
     assembly_gap    5359..5458
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5510..5740)
                     /locus_tag="PLT63_08340"
     CDS             complement(5510..5740)
                     /locus_tag="PLT63_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOD98770.1"
                     /translation="MKELIKYMAQALVDHPDQVEVSEVVGEQTSVIELRVAKEDLGKV
                     IGKQGRTAKAMRTVLSAASTKIRKRTVLEIIE"
     gene            complement(5814..6050)
                     /gene="rpsP"
                     /locus_tag="PLT63_08345"
     CDS             complement(5814..6050)
                     /gene="rpsP"
                     /locus_tag="PLT63_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOD98771.1"
                     /translation="MAVKIRLTRMGGHKKPFYRIVAADSESPRDGRFLEVLGYYDPAK
                     DMEGVSLKDDRIKDWLSKGAKPTLTVSQLLRKKG"
     gene            complement(6137..7471)
                     /gene="ffh"
                     /locus_tag="PLT63_08350"
     CDS             complement(6137..7471)
                     /gene="ffh"
                     /locus_tag="PLT63_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOD98772.1"
                     /translation="MFENLTEKLDAVFKKLKGRSRLDEENIQGALKEIRMALLEADVN
                     FKVVKSFIEDVKTRSLGQEVVESISPGQQVVKIVHDRLVELMGGTSSQLRFGSRFPAP
                     IMLVGLQGSGKTTTAVKLAKYITKQGKRTLLVSVDVYRPAAMDQLRILGKDASMPVFD
                     AGDVKDPVTICRRAVEGAKSRGYEALVIDTAGRLHIDLEMMAELSRIKEAVQPAEILF
                     VADAMTGQEAVNVAATFNERLGIDGVIMTKLDGDARGGAALSLKAVMGKPIKFVGVGE
                     KVEALEVFHPDRMATRILGMGDIISLVEKAQATVDEQQARELEKKIRKNEFNLEDFRN
                     QLLQIKKMGSLKDIFSMIPGMNKIKALKQVDPDDKELVRVTAIIDSMTKKERQNHLLI
                     DGQRRKRIALGSGTTVQDVNRLLKNYGDMRKIMKKMTSKEGMKALRRGNFPF"
BASE COUNT         1415 a         2357 c         2066 g         1547 t
ORIGIN      
        1 ccgatcatcg cccaactggc ctgcatggag gtgggaaaga cccgggacgg ccattccgtc
       61 acggaggcgt tccgtatcct gaccgatgcc ggggccgaca tcatcggcct caactgccgg
      121 ctgggtcccg ccgaactcct gcgcaccctg gaacagtctg ttgttcttcc agacacgctc
      181 ctctccgtgt ttcccaacgc cggtcgcctc ggcatcatgg aaggggaatt cggctatact
      241 tcgtctcccg cctacttcgc cgatcgggcc cggcatttcc acgcccaggg agcccggatc
      301 atcggcggct gctgcggcac caccccggat cacatccggg cggtatccgc agttcttaag
      361 gatctccggc ccctggcccg catcaatccg gaagcaccgc cggaaccggc gccccgtccc
      421 gccgttatct caagcggggg gaccggaacc ataacggaca aggtaaagga atggcccacg
      481 gtgctcgtgg aattcgaccc acccaaagac ctggatatcg agcgttacat gatcggggcg
      541 gcctcgctgc aggatgcggg ggcggactcc attaccatgg ccgacaactc cctggcccag
      601 acgcggatga gcaatctggc cctgggagcc atcgtcaaga tgcgcctggg catcgatccc
      661 ctggtgcaca tcgcctgccg ggaccgcaat ctcattggcc agcagtcgca tctcatgggg
      721 ttgcatgccc tgagcatcca ccaaatcctc gtcgtcaccg gcgatccggc gcgctatgga
      781 gaccttcccg gcgcgagttc cgtcttcgac gtctcctcat tcgacctgat ccgcatggtc
      841 aaacagctta acgccggtat ttccttttcc ggccagccgc tccgggagcg ggggcgtttc
      901 gtcgtcgggg ccgccttcaa tccccatgtc accaatctca ccggcgcctt caaccgtctg
      961 gggaaaaaga tcgaagcggg ggcggatttc attctgaccc agccggtata tgaccgggga
     1021 actatcgaga tgatcgccga gcggacccgg catctgcccg tgcccatctt catcggcatc
     1081 atgcccataa ccagcgcccg caatgcccaa ttcctgcaca acgaggtgcc ggggatccgc
     1141 ctcaccgaca cggtgctcaa gcgtatgggc aagtaccccg ccgcgggccc cgatgcccgc
     1201 cgggaaggtc ttgacctggc gcgggagctt gtggatgcgg cccgggaatg cttccgggga
     1261 ttttatctga tgacgccgtt ttcctattac gagctgactg ccacgctgac ccggcatgtt
     1321 aaggacggtg gcggcgggtg accggaaccg gggagcgatg gcgacccgcg cggtccatac
     1381 ccaggggttt ttatctggag ttcacaacca cgggcgttga acatcttcag gccagcggca
     1441 tctattccaa cgcggatatg agcgagcggc ggcgctgcct cggggaatga gatctatggc
     1501 caaacgagac aaatatacct tcagtttctg ggaaaagcta ttgctgcacc tggcgccgcc
     1561 catagccgcc ggggcaataa agctctggaa ccgcacctgc cgggtggtcg tccgggagaa
     1621 tgaagaacgg gagctcgccg ccctgcatga gaacggcggc tgcatctatg caacctggca
     1681 tcagcggatg ttctggtttt tccatgactt cggctcccgc cacgtcacca tgatgattag
     1741 ccgcagcaag gacggtgaat atgccaatgc cgtggccctg cgcctgggct tctattccgt
     1801 ccggggcagc aattccctga aggggcgcga ggctatgtat tatctcgtgg agctgctgaa
     1861 agagcgggcc ggccacaagg cggggatgat ggcggatgga ccgaacgggc cgccccgaca
     1921 gctcaagatg ggaaccatta aaatcgccag ggaaaccgga ctgcccatca tccctatgat
     1981 gtacggcgcc aagcggcgca tcgtctttca atcctgggac cgctacttcc tcccttgtcc
     2041 atttacggac atcgtcgtcc ttcacggcga gccggttttc gttcctgtcg atgccgacga
     2101 agaggaatgc gagcgcctgc ggcgcctggt caccgaccgt atgaacgaga tggccgaccg
     2161 ctgcgacacc tggtggggag ggatcccggt gggcaaaccg ggtttcgacc tgcctgcctc
     2221 ctcctgacac aaaccggcct gcgcaagcac cgggaaggca aatcattccg gaagctccta
     2281 tcgccggaga tgtcatgctt ttctaacatt gccggcaccg tgccggctaa cagttatcca
     2341 tttatttaaa tatattcagg aaatcaggtc aaaggcgtcg cggatcaact ccagctccgg
     2401 accggagggg aggagatgca ccgccaccac gtcgaaccgg gcggggacgt cgaacaggcc
     2461 gtgacggtgg agataaaagg cggcgaggcg ggacagtttc tgccgcttgg aaaatcccac
     2521 gctttcctcg ggggagccga aacgggcaga ccggcgggtg cgaacttcca caaacaccag
     2581 ggtctccccg tcccgggcca cgatatccaa ttcgcccagg gggcagcgcc agttacgggc
     2641 cagaatcgtg tagccgaccc gggctagaca gtccgcagca agctcctctc cccgccgtcc
     2701 ggcgctgatc ctatccctgg tcattggaag ataagctcgt aattatcttg agttgtatct
     2761 ttaatgtgga aggaccggcg atggatcttg cagaagccgt gctcccggat cgcccggcgg
     2821 tgctcggcgg ttccatagcc cttgttcttg aagaaattgt agtagggaaa ctggcggtga
     2881 tagatctcca tgatccgatc ccggctgacc ttggcgacga tggaggcggc ggcgatggag
     2941 acgctccgac cgtccccctt gacgatcgtt tcctgaggaa tctccagggg aacgctctgg
     3001 ttgccgtcca cgaggaggca gtcgggaacg acctggagat cggcgacggc ctcccgcatc
     3061 gccaggaggg tcgcccgaag gatgttgagc tcatctatga cccgggagtc caccaccccg
     3121 atccccacgg ccagggcgtc ggtgcggatc acctcgaaaa ggagctcccg gctccgggag
     3181 gtgagttttt ttgaatcgtt gatgtcgggg tgggaatatc gatccggaag aacgacggcg
     3241 gcggcaacga cgggaccggc caggggtccc cgacccgctt cgtcgatccc cgcaatgcgc
     3301 ctgaatcctc gttgccgggc ccgttcttca aaataattca tgtcttaaag acaatggtca
     3361 cgacttccct tcctctttga tccgggcctt tttacctttc aggccccgga ggtaatacag
     3421 gcgggaacgt cggacccggc cccgggtgac gacctcgatc cggtcgatga tcggggaatg
     3481 aagcgggaag gtacgctcca caccgatccc gtaagagatc ttcctgacgg tgaagctgct
     3541 ggaacagctc ccgcgccgct ttctaatgac gacgccttcg aaggcctgta tgcgggactt
     3601 ctgaccttcg acgatccgga cgaagacctt gatgctgtcc cccggcttga acgcgggcag
     3661 atccgtcctt atctgttctt tctcaatcaa ttcaactaca ttcataactc tcaacacctc
     3721 gcgtgattat tgtctgccat ctaaaataca accctgtttg ccgtcctctc ccggcgcacg
     3781 gccgaaagcc agcgcttaca caatcggacg gcatattatg agcattaagc ttcgttccct
     3841 tccgccagcc ccgacgcgga ccctcagcac ccaaagagcc tgtccagcac caccgccacg
     3901 gcggagcgta cggacaggtg gttatagccg tccgaaccgc cccgcaccgg gggcagggag
     3961 caatcggccc gggcaacgat gctgtccgcc agaccccatc cggtgccgaa aaccaacagc
     4021 cagggggtgt ctccttccac aagcgccttc cgcaactctc cgtaggatat caatcccttc
     4081 tccagagcgg cgccggtggc gaccacccgg ggtttcttac cggcgcgctc cccaatatcc
     4141 cgacagacct gctcgaaatc ttcccggacg cacacccggg aaaaggcctc ccgtcgagag
     4201 tggttgtact ccgccccgta tccccgccgc cagtgattga taatggtctc gaccagagac
     4261 cgctgcgccg cgatgggcgt cacgatataa tagtgggcga tgccgtaagt ccgggccgcc
     4321 ctggcgatat cgtgtatatc caggttggcc accgcggtgg tcacaacctg ccggtgcttg
     4381 ttcagaaccg gataatgcaa aagagcgaga taacattcag gctgcgccgc cacgataata
     4441 ccccgggaca tacattcccc cgcctggcgt acggatcatt tttcagacgg tacgggcggg
     4501 tctgtttcac cttaccgggc tgcgccatca tctccccgga cgtgtttccc ggcgatcctc
     4561 tttttccgcc gcctccagct cccggaggaa tgtccggtca gcctcggaaa gctccgcccc
     4621 ggccagcagt tcgggacggc ggcgccaggt ccgcagcagg gattgtcggc ggcgccaatt
     4681 ttcaatttcc cggtgatttc cgttcaccag gacctccgga acttcccagc cccggtactt
     4741 ttggggtcga gtatattggg gatattcgag gagtccggcg gcgaaggaat ccgcgcgggc
     4801 ggattcgctg ttgcccagga cgccgggaat gagccgggcc accgcatcga cgaccaccat
     4861 cgccgcaagt tcgcccccgg tgaggacata gtcgccgatg gaaagttcgc ggtttaccag
     4921 atgttcccgg acccgctcat cgacaccctc gtaatggccg cagatcaata aaatccgctc
     4981 acagacggct agttccgtgg cgatttcctg gcggaacggt gcgccctggg gactcatgag
     5041 gatcaccggg cccgcctcgg acggcatcgc cagggcttcc agggccaggg ccaggggttc
     5101 caccttcatg accatgcccc cacccccgcc gtagggtgcg tcatcggtca cccggtgctt
     5161 gcccggggca tagctcctga tgtcatggac ctggaggtcg attagccccc gttcccgggc
     5221 cttcccgagg atgctgcccg cgaagggaga aacgaacatc tccgggaaaa gggtgaggat
     5281 atcgaagcgc atccaccggc ctcacaaacc tgcaagcagc cttaccgtaa tgatccggtt
     5341 ggtccggtcc acgttcctnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg
     5461 gcagccgtgg accttgacga tttcgccgat ttcaaagaac tccgtcaccc tattcgataa
     5521 tttccagaac ggtccgtttc cggatcttgg tggaagccgc gctcaaaacc gtacgcatag
     5581 cttttgcggt tctcccctgt ttgccgataa ccttgcccag gtcttccttt gctaccctca
     5641 gttcgatgac agacgtctgc tcgccgacca cctcggatac ctcgacctga tcggggtgat
     5701 ccaccaatgc ctgcgccatg tatttgatca actctttcat cgctttcacc tccactggat
     5761 tagtccgtta aaccttttca agatgtcgac tcgaagagcc cattgcccgg cttttatccc
     5821 tttttcctca atagctgaga tacggtgagg gtaggtttgg cgcccttgga cagccaatcc
     5881 ttgatccggt cgtctttgag acttacgccc tccatgtcct tggcgggatc ataatacccc
     5941 aaaacttcca ggaaacggcc gtcacgcggc gattccgaat ccgccgccac aatcctgtaa
     6001 aacggtttct tgtgtccacc catgcgggtc aatcttattt ttaccgccat cctctttacc
     6061 gatcacctcc tgttgttgtt cttgccggca ttttgccgat cgaaagatcg gaccatatat
     6121 catatctttt tcttatttaa aagggaaaat ttccccgacg gagggccttc attccctcct
     6181 tggaagtcat cttcttcatg atcttccgca tatccccata gttcttcaag aggcggttga
     6241 cgtcctgaac cgtggtaccg ctccccaggg caatgcgctt ccgtcgctgg ccatcgatca
     6301 ggagatggtt ctggcgctcc tttttggtca tggaatcgat gatcgccgtc acccggacca
     6361 gttccttatc atcgggatcg acctgcttga gggccttgat cttgttcata cccggaatca
     6421 tggaaaagat gtccttcagg gatcccatct tcttgatctg cagcagttgg ttccgaaagt
     6481 cctccaggtt gaattcattc ttccggatct tcttttcaag ttcccgggcc tgctgctcat
     6541 ctaccgtggc ctgggccttt tccaccaggg agatgatgtc tcccatcccc aggatccgcg
     6601 tcgccatgcg atccggatgg aagacttcca gggcctccac cttttccccg acgcccacga
     6661 atttgatggg cttgcccatg acggccttca gggacaaagc ggcgcctccc cgggcgtcgc
     6721 catccagctt ggtcatgatg acgccgtcga tccccagtcg ctcgttgaag gtcgcggcga
     6781 cgttcacggc ctcctggccg gtcatggcat cggcgacaaa gaggatctcc gcgggctgga
     6841 cggcctcttt gatgcggctc aattccgcca tcatctccag gtcgatatgg agccgcccgg
     6901 cggtgtcgat gacgagcgcc tcgtaccccc ggctcttggc gccttccacg gctcggcggc
     6961 atatcgtcac gggatctttg acatctcccg catcgaatac cggcatggag gcatctttcc
     7021 ccaggatccg caactgatcc atggcggcgg gacgatagac gtccaccgat accagcaggg
     7081 tcctctttcc ctgcttcgtg atgtatttgg caagtttgac ggcggtggtc gtcttgccgc
     7141 ttccctggag gcccaccagc atgatcggcg cggggaaacg gcttccgaac cggagctggc
     7201 tgctcgtccc ccccatcagt tccaccaggc ggtcatgcac gatcttcact acctgctgcc
     7261 caggactgat gctttcgacg acctcctgac ccagggagcg ggtcttgacg tcctcgatga
     7321 aactcttcac caccttgaaa ttgacatccg cctccagaag ggccatgcgg atctccttca
     7381 gggcgccctg gatgttctct tcatccaggc ggctccggcc cttcagtttc ttgaagacgg
     7441 catcgagttt ttcggtaaga ttttcaaaca taaaccacct tgttg
//
LOCUS       DAOSMF010000088         7340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_11231_length_7340_cov_23.078518, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000088 DAOSMF010000000
VERSION     DAOSMF010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 7340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7340
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11231_length_7340_cov_23.078518"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            320..1264
                     /gene="ftsY"
                     /locus_tag="PLT63_08355"
     CDS             320..1264
                     /gene="ftsY"
                     /locus_tag="PLT63_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOD98773.1"
                     /translation="MLDVFKKKGFFDKLKAGLAKTRDILLTDVDDLILGEKKIDQELF
                     DELEEVLIRADIGPAFTYELIEKMKDQVKRKELSRADLLKKILRDTIKEILTGQDAAL
                     KIPPEGIYTVMVVGVNGTGKTTTIGKIAYELKSRGERVLLVAADTFRAAAIEQLEVWS
                     NRAGAPLIKQRPNADPAAVVFDAIQAAKAGKGRILIVDTAGRLHTKLNLMEELKKMKR
                     IVARELPGGPQEVLLVVDATTGQNGLVQAKMFKEEVGVTGIVLTKLDGTAKGGIVIRI
                     AKELNIPIRYIGVGEGLEDLQPFDGNAFVEALFGAERQ"
     gene            1272..2198
                     /gene="ftcD"
                     /locus_tag="PLT63_08360"
     CDS             1272..2198
                     /gene="ftcD"
                     /locus_tag="PLT63_08360"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HOD98774.1"
                     /translation="MKILECVPNFSEGRSPKKVREITAAAAAVPGLSILDYSWDTDHH
                     RSVVTFLGAPEDVYEAALAAGGKALEIIDLRGHDGVHPRIGAVDVVPFIPLAETQMAE
                     AVYWAHRFGRTFAERHGVPVFFYGEAAVNPARRQLPDIRRGGLAGLIDRLCNRGWYPD
                     AGPSACPERAGAVAVGARMPLIAYNINLKTADIALAKRIAGRIREANGGLPGVRAIGV
                     FLASRKLAQVSMNLTDFRVTSLQDAFDFVSREAAAAGMEVLESELIGLAPQAALNAET
                     AAAILLRDFSPARIIETHLRNVEEPRAGADPP"
     gene            complement(2365..2565)
                     /locus_tag="PLT63_08365"
     CDS             complement(2365..2565)
                     /locus_tag="PLT63_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HOD98775.1"
                     /translation="MPEGKVKWFNEQKGFGFIEKDDGGDVFVHYSTIQGSGFKTLQEG
                     QRVSFDIQQGKKGPAAENVKPL"
     gene            3019..4584
                     /locus_tag="PLT63_08370"
     CDS             3019..4584
                     /locus_tag="PLT63_08370"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HOD98776.1"
                     /translation="MMKRLKICLINPRFEPSYWGMDYALPIYPGSKLCTMTTGALPHL
                     AGLIPDHDVYLLDENVEEIDFGTMKDYDLVGVTGMIVQKDRMRSILLRLREIKVFTAV
                     GGAYASVEEDFFTGLCDVLFSGEADDTWPEFVAAFAAGSPCKKLYRQENRTVMTTLNK
                     PRYDLLKVDRYASGSVQFSRGCPFQCEFCDIIVTFGRRPRTKSPEQLLEELDDMRKVG
                     FFSCFIVDDNFIGDKKAAKALLRLIIPWQRKHGYPLRLMTEASINLADDAELLELMYQ
                     ANFRSVFIGIETPRLSSLQETKKYQNTSGGTLKEKIDRIRNAGLDINGGFIVGFDHDD
                     IAVLDDQFSFIQENGILLAMVGMLVAVPKTPLFKRLEKEGRLRREDPNCNIVPKQMSS
                     GELQQGYWDLLTRLYAPEAFLDRYFQVFLFPEFNRRRAEICDLANEGKRLPTLAYGLI
                     LLWNLFWTLFRDGSLGKVGRVYVRYFFGRSIGYRNDIIGFAQYMNRCATHWHFYKFTR
                     EGVAGRLRLFNSG"
     gene            complement(5559..6083)
                     /locus_tag="PLT63_08375"
     CDS             complement(5559..6083)
                     /locus_tag="PLT63_08375"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HOD98777.1"
                     /translation="MPIYEFAGKAPTIAKTAFIHPEAVVIGEVTIGTRCHIAAGAVIR
                     GDCGPIVIGPSTSIQDNATVHVNPGERVNVARNVIVGHNAVLHDVTIGERCVIGMGAI
                     LLPGVVCEREAIVAAGAVVSQGLHIPARKIVAGNPAKIIKDITPAMEAYVTEGIEQYN
                     RFTDLYRRTMKRLE"
     gene            6340..7062
                     /locus_tag="PLT63_08380"
     CDS             6340..7062
                     /locus_tag="PLT63_08380"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOD98778.1"
                     /translation="MGTIIDITLEEIRCIINEGLDSEGALRILKILDGPRYSKEFIEE
                     LTIYSELLPLGRERPFSPEQRYLHFLWDAFDKLPICLNANVGILFRRLIAERLFKRCG
                     DGFIAEENFRFNFGQYIEVGDFVFFNRGVFVDSKGGVIIGNNVAITEDVRIFTHSHSE
                     SSHIIREYKPVIIKDYAKIYTGAVILPGVTIGEQAIVASHSLVTKDVPDNTLVAGMPA
                     QVVRERKTEGRVGDDLDHIWLF"
BASE COUNT         1686 a         1921 c         2024 g         1709 t
ORIGIN      
        1 gcctctggag ctttttactt cgtcgtctcc atcatgactt tttacgaatt catcttgcgt
       61 tgcctttctc cccgcgtttt ccgtccgcgg agtccttctt tcccatgtgt cccccccttt
      121 tgtcttcgcc tcccgtcttt acttctccgg gaaaaacggt taaaggaggc ttgccttttt
      181 caacggaaaa aggggacggt gaaaaagggg gacagttctc tttttcggga accttacccc
      241 tcctcagatc cagagggcag gcgtgagaaa cgatgcttga tttctcccgt cggtcacagg
      301 aggaaaaacg aggtgaagca tgctggatgt atttaagaaa aaaggttttt ttgacaaact
      361 gaaggccgga ttggccaaga ccagggatat cctgctcacc gatgtggacg atctcattct
      421 gggagaaaaa aagatcgatc aggagctgtt tgacgagttg gaagaggtgc tgatccgtgc
      481 ggacatcggg ccggccttca cctatgaatt gatcgagaag atgaaggacc aggtcaaacg
      541 gaaagaatta agccgcgccg atttgttgaa gaagatcctg cgagatacca taaaggagat
      601 cctgacgggt caggatgccg ctttaaagat cccgcccgag gggatctaca ccgttatggt
      661 ggtgggggtc aacgggaccg gcaagaccac gaccatcggc aagattgcct atgaactgaa
      721 aagccggggg gagcgggtcc tcctggtggc cgcggacacc tttcgtgcgg cggccatcga
      781 gcaattggag gtttggagca accgcgccgg cgccccgctg atcaagcagc ggcccaacgc
      841 cgatccggcg gcggtggttt ttgacgccat ccaggcggcg aaagcgggga aagggcgaat
      901 cctcatcgtc gatacggcgg gacggctcca caccaagcta aatctcatgg aagaactgaa
      961 aaagatgaag cgcatcgtag cgagagagct cccgggcgga ccgcaggagg tgctcctggt
     1021 cgttgatgcc accacgggac aaaacgggct ggttcaggcc aagatgttca aggaggaggt
     1081 aggggtcacg ggcatcgtcc tgacgaaact cgacgggacg gccaagggcg ggatagtcat
     1141 ccggattgcc aaagagctta atatccctat tcgctatatc ggtgtcggcg aaggtctgga
     1201 agacctccag ccctttgacg gcaatgcctt tgtggaagcc cttttcggtg cggaaaggca
     1261 gtgaggcggc gatgaagatc ctcgaatgcg tccccaattt cagtgaaggc cggtcgccga
     1321 aaaaggtccg ggagattacg gcggccgcgg cggccgtccc cgggctaagc atcctggact
     1381 attcctggga caccgaccat caccgcagtg tcgtgacttt tctaggggcg ccggaagatg
     1441 tttatgaagc ggccctggcg gcaggcggca aggccctgga gataatcgac ctgagaggcc
     1501 atgacggggt tcacccccgg atcggcgccg tggacgtcgt gcccttcatc cccctggcag
     1561 agacgcaaat ggcggaagcg gtttactggg cgcatcgctt tggccgcacc tttgcggaac
     1621 gtcacggtgt ccctgttttc ttctatgggg aggcggcagt caatccggcc cgccggcagc
     1681 ttccggacat ccgccggggc ggactggcgg gtctgatcga ccggctgtgc aacagaggct
     1741 ggtatccgga tgccgggcca tccgcctgtc ccgagcgcgc cggggcggtg gcggtggggg
     1801 cccggatgcc cttgatcgcc tataacatca acctgaagac cgcggatatt gccttggcca
     1861 agcggattgc cgggcgaatc cgggaggcca acggcggtct gcccggggtc agggcaatcg
     1921 gggtctttct ggccagccgc aaactggccc aggtgtccat gaacctgacc gattttcgtg
     1981 tcacctccct ccaggatgcc tttgatttcg taagccgtga ggcggctgcg gcggggatgg
     2041 aggtcctgga gtcggaactg atcggcttgg ccccgcaggc cgccctgaat gcggaaacgg
     2101 ccgcggcgat ccttctgagg gatttttctc cggcaagaat aatcgagacc catctgcgga
     2161 atgtggaaga accgcgggcc ggtgcggatc caccctgaca cacatcggca aagctgggta
     2221 tcgttcataa tttccgccgg gtccgaccgc tacgaacctt ttccggtttc tgccagaaat
     2281 tctttccgga tcgggaagca agaagatcaa aaaaagacgc cgcggtcgat gaaaccgcgg
     2341 cgtcttgatc ggttacgctg agggttatag aggctttacg ttttccgctg ccggaccctt
     2401 cttgccctgc tggatgtcaa agcttacgcg ctggccttcc tgaagggtct taaaaccgga
     2461 accctggatg gtgctgtagt gaacaaaaac gtcgccgccg tcatcttttt cgatgaagcc
     2521 gaagcctttt tgctcattga accatttaac ttttccttct ggcatttcct gcatcctcct
     2581 ttctgtaaag tcggattgca tctctgacag aaagataagc tccgctgtac ttcaaaatgc
     2641 ctttttccaa tctttactac ttgttctccc ccccgcttgt cacagcggcg gagataagat
     2701 ctatgggtcg gcatatagta cggcgcctga gcgctgtcaa gcattttgtc aaaatatttt
     2761 catgcccaag attcggtgaa gatgccgaag atatttacat cgtttgtggc gcagccatgg
     2821 agaagaaagg ttttgtaaaa cgttaatcaa taattcttga ctttgttggg aaacagacat
     2881 attaaaagtt ggatgtgtct gccgccatag ggcggcgccg gcccgaaagc ataagacgag
     2941 gcctcacaaa agagattttg aagtgtcatg gacagccgcc atgttctttg agatggcggc
     3001 tttttttgta cctcctgaat gatgaaacga ctgaagattt gcctgatcaa tccccggttc
     3061 gagccttcct attgggggat ggactacgcc ctgcccattt accccggcag caagctgtgc
     3121 actatgacca ccggcgcctt gccccatctg gccgggctga tcccggatca cgacgtctat
     3181 cttctcgatg aaaatgtcga ggaaatcgat ttcgggacca tgaaggatta cgatctggtg
     3241 ggcgttacgg gcatgatcgt ccagaaggac cggatgcgct ccattctcct ccgtctccgg
     3301 gaaataaagg ttttcaccgc cgtgggcggc gcctatgcct ctgtggagga ggatttcttc
     3361 accggcctgt gcgatgtgct tttttccggc gaggccgatg atacctggcc cgagtttgtg
     3421 gccgccttcg ctgccggcag tccgtgtaaa aaactctacc gccaggaaaa tcgaacagtg
     3481 atgaccaccc tgaataaacc gcgctacgac ctcctgaagg ttgaccggta cgcgtcggga
     3541 tcggttcagt tttcacgcgg ttgtcctttt caatgcgagt tttgcgacat tattgttacc
     3601 tttggacgcc gcccgcggac caagagcccg gaacagcttc tcgaagagct ggacgatatg
     3661 cgcaaagtcg gatttttctc ctgctttatc gtggacgaca atttcatcgg cgacaaaaag
     3721 gcggccaagg cattgctccg gctcatcatc ccgtggcagc gcaaacacgg ttatcccctg
     3781 cggctcatga cggaggcaag catcaacctg gccgacgacg cggaactgct ggaactcatg
     3841 tatcaggcaa attttcgctc cgtcttcatc ggtatcgaga cgccgcgcct gtcgtccctc
     3901 caggagacga agaaatatca gaacacctcg ggggggaccc tgaaggaaaa aatcgaccgg
     3961 atccggaatg ccggtctgga catcaacggc ggcttcatcg tcgggttcga tcatgacgac
     4021 atcgcggtgt tggatgatca attctccttc attcaggaaa acggcattct cctggccatg
     4081 gtgggtatgc tggtggccgt gcccaagacg ccgctcttca aacgcctgga aaaggagggt
     4141 cggctgagaa gggaagatcc caactgcaat atcgtgccca aacagatgtc ctccggagaa
     4201 ctccagcagg gctactggga tctcctcacc cgcctctacg cgcccgaggc ctttctcgac
     4261 cgttatttcc aggtttttct gtttcccgag ttcaaccgcc ggcgggcgga gatatgcgat
     4321 ctggccaacg aagggaaaag acttcccact ctggcctacg gtttgatcct cttatggaat
     4381 ctgttctgga cactcttccg ggacggcagc ttgggcaagg tcggaagagt ctatgtccga
     4441 tatttttttg gccgcagtat cggttatcga aacgacatca tcggtttcgc ccagtatatg
     4501 aaccgatgtg caacacattg gcatttctac aagtttaccc gggaaggggt ggcgggacgc
     4561 ctgcgcctgt tcaattccgg ttgagaaatc atcagacaaa ttccgatctg aatcgtcaaa
     4621 aatccttacc cgagccgtca cctttttccc agcccatatt catccggagc ccgattgttg
     4681 aaaatggcgt tccggattat actgcccccg aaagaaacaa aggagaaaaa accatgaaaa
     4741 ccatcctcgg tgcacgaatc gaattcaatc cccagcgcga cgtcctgggg aagatgatag
     4801 agagaaatcg gaacggtttt aataatcatc cagccggtct gaaaggatta taacccggag
     4861 cggggaagga ggcgaaagcc atgagaaaga ccggtgccag agacagagaa agattcgcca
     4921 agaaggcggg caagacctgg ttggatccca tgtgcacccc ggtggaaccc cggtaacggc
     4981 gggttcacgg cagaggtgcc ggttgttaac cgcagagaga gagaggagga gcaaatcatg
     5041 aagaaggata aacggacata ccgggattgg acggacccca tgtgcacgcc ttatcggtat
     5101 aaaaaaggct gagcgtacgg ggaacccgca tatcggggcc gccgtaaata ttggcggtgc
     5161 ttgccggaag aggcgataag aaacggaacg tgcggatacc tgcctctctc aaaaattaag
     5221 ggcggagtcg atgagacttc gcccttagtt ttttaaaacg ccggggacat gccggcggcg
     5281 gttaccgaac ccgacaaaat cgctctcgtg gagacgccgg tgataaatga gtgcatgcca
     5341 acctccttgc ctcgactaat gccccgatag accttttcac ggggccgtag tttctgttat
     5401 tgacggcatc gtaaaaagtc cggatgcttc gaaagcgctt catccttcgt cacggcgacg
     5461 tacgtcccag tacttatcat tcctaatgat tatagcgtct taactgctga gcattttacg
     5521 atgccgtccc aaatgggaca ttttgggctc gctacttctt attccagcct tttcatggtg
     5581 cgccgataca gatcggtgaa acggttgtat tgttcaatcc cctccgtcac atatgcttcc
     5641 atggctggtg ttatgtcctt gattatcttc gccggattgc cggccacgat ttttcttgcc
     5701 ggaatatgca ggccttgtga gaccaccgct ccggccgcga cgatggcctc acgctcgcag
     5761 acgaccccgg gcaggaggat ggcccccatg ccgatgacgc atcgctcccc gatggtcaca
     5821 tcgtgaagga cggcattatg gccgacgatc acgtttctgg ccacgttcac cctttctccg
     5881 gggttgacat gcactgtggc attgtcctgg atgcttgtcg acgggccgat tacgatcggg
     5941 ccgcagtcgc cgcggatcac cgccccggcg gcaatgtggc atcgagtgcc gatcgttact
     6001 tcgccgatga ccaccgcctc cggatggatg aacgcggtct tcgctatggt cggcgccttt
     6061 ccggcgaatt catagatggg catggttatt cctctatttc ctaatcacct tgatatttct
     6121 cggttgttaa tgtaaccatt tcccccaggg atctcaaaaa gccccataaa aggcgggaca
     6181 gaatgagact aaaaacaggc taagcgaatt acgattaatc gatcgcataa atcaagtaaa
     6241 aatgtgtctg atatataggc ccgtttgcca tcgcttatac tgccgctata atccttgctg
     6301 ctttcgccgc cgacaatgaa agcgaggtca aattaagaca tgggaacaat tatcgacata
     6361 acccttgaag aaattcgctg tattataaat gaagggctgg attcggaagg cgcgcttcgt
     6421 attctcaaga ttcttgatgg tccccggtat tcaaaggaat ttatagaaga acttactatc
     6481 tatagcgaac tgcttccatt gggccgcgaa aggccctttt ccccggagca gcgttatctg
     6541 catttcctgt gggatgcctt cgacaagctg cccatctgcc tcaacgcaaa tgtcggaatc
     6601 cttttccgta ggcttatcgc tgagcgactt ttcaaaagat gcggcgacgg ctttattgcc
     6661 gaagagaatt ttaggttcaa ttttgggcaa tatattgagg ttggagattt tgtctttttc
     6721 aaccgcgggg tctttgtcga ttcaaaagga ggcgtaatta tcggcaacaa tgttgctata
     6781 acagaagatg tgaggatatt cacccacagc cattctgaat catcgcacat catcagggag
     6841 tataaaccag taattataaa agattacgcc aaaatttaca ccggtgccgt cattctgcct
     6901 ggtgtcacca taggagaaca ggctatcgtg gcatcacatt ccctggttac aaaagacgtt
     6961 cctgacaata cactcgtggc cgggatgccg gcacaggtgg tccgggaacg gaagacagag
     7021 gggagagtcg gcgatgatct ggatcatata tggctttttt agtaaggcag gttcctcttt
     7081 gttatcctaa aaactatacc acctctttct atatttactg atgcccccgg ggcgattcga
     7141 acgcgcggca cacggattag gaattaaaaa taccctatcc aaaacaggct aaatatgcga
     7201 ttttacagcg ttgtgctttg gctctcttgg ctataattta gtcaagagag ccactttttt
     7261 tactttttta cccaccaagt cacccactat ttcttcggtg ggtttgaggg ttttggcgca
     7321 ggtggcgatg gcggaacata
//
LOCUS       DAOSMF010000089         7305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_11298_length_7305_cov_15.459586, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000089 DAOSMF010000000
VERSION     DAOSMF010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 7305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7305
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11298_length_7305_cov_15.459586"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="PLT63_08385"
     CDS             <1..320
                     /locus_tag="PLT63_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98779.1"
                     /translation="SDYESESRTFESCRARQYNQGVNQIMVSPFPVDVHWQYIPSSRR
                     FSWIRFLDIFFPNVLRGVYLLKGHRYILTTVPEMVFLVDTERDYDGSVFVDVGFVLII
                     HID"
     gene            complement(436..693)
                     /locus_tag="PLT63_08390"
     CDS             complement(436..693)
                     /locus_tag="PLT63_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98780.1"
                     /translation="MERLSDKKAKRLILNVSPKATHYNNAQSEKGEDRDEGSGGKKGC
                     NLPTDQAMELEFRLGMEVINGSELREGVNKFSQGMGKHGQF"
     gene            complement(1494..3728)
                     /locus_tag="PLT63_08395"
     CDS             complement(1494..3728)
                     /locus_tag="PLT63_08395"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOD98781.1"
                     /translation="MGTVHRTGCVLCPQNCGLEVEVENNRIVKVRGDKGNVRSLGYVC
                     RKGLNIANHQHNADRLKYPLKRVGDRFERISWDQAIDEIAAKLKEIVGEYGPKSFAYM
                     GGGGQGCHFEAAFGVRLMRGLGSRYHYSPLTQEFSGLFWVNGRIYGRQNVHTEIDAEE
                     TDFLLVFGWNGMESHQIPQAPRQLQKIARDPNRQLVVVDPRRSETAKVADIHLPIRPG
                     TDALLMRAMIAVILQEGWENREYMERHTSGMEAIRDLFMSFDAPRAVKTCHLDFDQVR
                     EICRQFATRRSCLRYDLGIMMNRHSAVSSYLAATLLAICGRIGVRGGNIFHGQMIPLG
                     SHSDERDPRTWRTVATNFPAIVGCFPPAAMPEEILSDRPDRLRAVIVSGAHPLRSYPD
                     TTAYEEAFKRLDLLVTAELAMTETAVLSHYILPARSGYESWDGTFFPLTFPGIYFQMR
                     RPIIEPEGEPLELGEIHLRLADRLGLIPPIPESLYRAAEEGHVAFGLALMEYAGKEPA
                     AMGAMPFILGKTLGKAKGSVHLAALWGMMQTAPKALRKNAARAGFKPGMMMGEELFQK
                     IMDHPEGLWIGRVDPEDNLNSLRTEDGKINLEIPELRAELEGLDPECEEAALMPAPVF
                     PFVLMAGRHFRMNANTLMRNPVWNEGKRDCTLLIHGDDAAALGIRDGQTVRVTTEAGS
                     VEIEAEVTETAHRGHVVIPHGFGMVYEGKVHGVNVNRLTKNTNRDWLGTPMHRYVPCR
                     IEAI"
     gene            complement(3764..4126)
                     /locus_tag="PLT63_08400"
     CDS             complement(3764..4126)
                     /locus_tag="PLT63_08400"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOD98782.1"
                     /translation="MAQKVAELNITPVQALVLGFLNEEDGISSSELGKRAELDSATMT
                     GIIDRLETAQLIERHGNPADRRSIRIHLTEHGRKLAGVAVRLIVEANREFLGFLTESE
                     QMELRSLMNKIRDQAGRG"
     gene            4545..5483
                     /locus_tag="PLT63_08405"
     CDS             4545..5483
                     /locus_tag="PLT63_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98783.1"
                     /translation="MRGKKITLIVWLALFLFFYGAVAGAAAATPAEGYKKAEQKTEPK
                     AEPVKPTADLTVSVLSNYISKGDELSRNSIVIQPEVIVGYKGFSLAIWGNLDTRPYLS
                     ETSTYKGDKRQNWTETDLTLAYNGSFGIVNAGMAYAYFGNAAFWNSDATGAGPDMKDD
                     QEVSLILGLNTILAPKLKVGRSFDSTQIWYLSLGVSHAFQINEYLGLTLAATGSYLVS
                     QDENRLKINSNGTDRKPDGSLNDRYNNFHDAVISLTLPVKPTAYLTITPQLSYIFPMV
                     DDAKYDMMFRSMQCDTTFSDRQSSYLVGGVSATLSF"
     gene            complement(5499..6404)
                     /gene="zupT"
                     /locus_tag="PLT63_08410"
     CDS             complement(5499..6404)
                     /gene="zupT"
                     /locus_tag="PLT63_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HOD98784.1"
                     /translation="MNEFWTAMGLTVFAGMATGVGSVIAFTAGRTNYRFLSAATGFSA
                     GIMLYVSFVEIFPEGLAVLSLHYGADRGPWFNAAAFFGGMLIIGLIDNLVPAAVNPHE
                     THAEAEARPLHDPAAPPPASAASALSGPEKAAAGFHNHGRPEYNLMRMGLFTALAIGI
                     HNFPEGMVTFLAALEDTALGIVIALAVALHNIPEGISVSVPIYYATGNRRKAFLYSLA
                     SGLAEPVGAVIGYLAILFFLGGQGEAIPPQLMGLLFGGIAGVMVYISLDELLPASRAY
                     GKGHDSLLGLVAGMLVMALSLLLLK"
     gene            complement(6401..>7305)
                     /locus_tag="PLT63_08415"
     CDS             complement(6401..>7305)
                     /locus_tag="PLT63_08415"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOD98785.1"
                     /translation="GLAFAVLCWAANTVLARGVIQEVHPMALSFWRWVFALVMIFPFG
                     FLSVKRDLPVIKSNILPLVVLSFFSVAVYNSLLYVAAHFTTANNMSIVTATMPAITIV
                     AAWLIIHERPLVFQILGVVISTTGMLWIIFQGSPANVRNLVFNYGDLLVIVSVASWSL
                     YSVFLRKFSIVLHPLSLLTMTIALGLLFIAPFYIGEIIVTGTFSFTSRVLPVFLFLGL
                     FPSILAYLCWNIGVGRVGPSTAAMFLYLLPIFAAFLSFGFLGERISAYHLLGGALIFI
                     GLYLAKPKNPERKNMFYQKDRDKG"
BASE COUNT         1565 a         2000 c         2062 g         1678 t
ORIGIN      
        1 cgtcagacta cgaatctgaa agtcgcacgt tcgaatcgtg ccgggcgcgc caatataatc
       61 aaggggttaa ccaaattatg gttagcccct ttcccgttga tgttcattgg caatacatcc
      121 ccagctcacg ccgattttcc tggatacggt ttcttgatat attttttcca aatgtcctcc
      181 ggggtgttta tctcctgaag ggtcacagat atatcctgac caccgttcca gaaatggtat
      241 ttctggtcga tactgaaagg gattacgatg gttccgtttt tgtcgatgta gggtttgtct
      301 tgattatcca tattgattga tgtcctttat ttatgattgt tatcggcaag agcttggtca
      361 tgactcaatt aaaattcctg acgctttttc tctcatgaat tactgaaaat atgtccttca
      421 tattcgcatc cttgtctaaa attgaccgtg ctttcccata ccttgcgaaa acttgtttac
      481 tccttcccgt agctcgctgc cgttaatcac ttccattccc aggcgaaatt ccagttccat
      541 tgcctgatct gtcggcagat tgcatccttt ttttccacca gaaccttcat ctctgtcctc
      601 tcccttttcc gactgtgcat tgttataatg ggtcgccttg ggactaacgt tgaggatcaa
      661 ccgcttcgcc tttttatcag ataatctttc catttcccac ttgataatca gaataaaaaa
      721 gggcgaagtc tgttcaactc cgccctttac cttgagagag gcaggtatca atcatgtccg
      781 tttttgccgc cgctctcgcc agatcttgcc gcaggggaag atcttatatc tgctttgcta
      841 tcagcctttc ttgtactgat aaggcgtagc catcgggtca acccaaccct ggaatgcttt
      901 cttttccttc ttcatgatcc ttgcctcctc tcttgatttg tgaatcctgc ttcttttcct
      961 gtctcaagtt accgggtttc catcggggtg cacatggggt ccaaccaggt cttgcctgcc
     1021 ttcttggcga atctctcttc tttcttggct cctgtcttct tcatggtcct atctcctctc
     1081 tctcaataaa ttttataatc ctttgagacc ggcctgatga aaacggccgg tcatgtttct
     1141 ttctatcgtc tttcccagga tgtctctccc gggagtgaac tcgatccttc gggcgccggt
     1201 ggttgtcatg gtctttatgt ctcctctgtc tttctttcgg ccctaagata atcccgaaaa
     1261 caatttttaa caatcagggt aggtctgaat atggaatggg aaaacaatgc agagaaatct
     1321 cggtaacggt ggcgaagaag atcggatttt gtccgatgga ttgtgtgatt ggtaattatg
     1381 gatatcggca agatctgcca ccaaggaatg taataggtga gggaattctc aggaatgacc
     1441 tgcccaaatc acttttgagg ggccttccat attccttcaa ctatttcaga aattcaaatg
     1501 gcctctatcc ggcatgggac atagcggtgc atgggcgtcc ccagccaatc acggttcgta
     1561 ttcttcgtca gtcggtttac gttcacgcca tggaccttgc cctcgtaaac catgccgaag
     1621 ccatggggaa taacgacgtg gccccgatgt gcggtttccg ttacctccgc ttcgatctcc
     1681 acggaaccgg cctccgtcgt gacccggacc gtctggccgt ccctgatgcc aagcgccgcc
     1741 gcatcgtctc cgtgaatcaa caaggtgcag tcacgcttgc cttcattcca gacagggttg
     1801 cgcatcaggg tgttggcatt catccggaaa tgcctcccgg ccatgaggac gaaggggaag
     1861 acgggagcgg gcatcagggc cgcctcctcg cactccgggt cgaggccttc caactcggcc
     1921 cgaagctccg gaatttcgag gtttatcttt ccgtcctccg tccgtaagga gttgaggttg
     1981 tcctccgggt ccacccggcc gatccagagt ccctccggat gatccatgat cttctggaac
     2041 agttcctctc ccatcatcat ccccggtttg aatccggccc gggcggcgtt tttacgtaag
     2101 gccttggggg cggtctgcat catcccccac agggcggcga gatggacgga ccccttggcc
     2161 ttgcccaggg tttttcctaa aatgaacggc atggcgccca tggccgccgg ttccttcccg
     2221 gcatattcca tcagggcgag gccgaaggcg acgtgaccct cttcagcggc ccggtacagg
     2281 ctttccggga tcggggggat cagtcccagg cgatcggcga ggcgcagatg gatctcaccc
     2341 agctccaggg gttccccttc cggttcgata atcggcctgc gcatctggaa gtatatcccc
     2401 ggaaaggtca gcgggaagaa ggtgccgtcc caggactcat atcccgaccg cgccgggagg
     2461 atatagtggg ataaaacggc cgtctccgtc atggccaact cggcggtcac gaggagatcc
     2521 agacgtttga acgcctcctc gtaggccgtt gtatcgggat aggaacggag gggatgggcg
     2581 ccggagacga tgacggctcg gaggcgatcg ggacggtcgg acaggatttc ctccggcatg
     2641 gcggcaggag ggaaacagcc cacgatggcg ggaaaattcg tcgccactgt ccgccaggtc
     2701 ctgggatcgc gctcgtcgga gtggcttccc agggggatca tctggccgtg gaagatgttg
     2761 cccccacgaa cgccgatccg gccgcagatt gccaggaggg tcgcggcgag gtaagacgac
     2821 acggcgctgt ggcggttcat catgatcccc aggtcgtagc gcaggcatga tcggcgggtc
     2881 gcaaactgcc ggcatatctc gcgtacctgg tcaaaatcga ggtggcatgt ttttacggcc
     2941 cgtggggcgt caaaactcat aaacaggtcc cggattgcct ccatgccgga ggtgtgcctt
     3001 tccatgtatt ccctgttttc ccatccctcc tgaaggatga cggcaatcat ggcccgcatg
     3061 aggagggcat cggtccccgg acggatgggc agatggatat cggcgacttt ggcggtttcg
     3121 gaccggcggg gatcgacgac gacgagctgt cggttcggat ccctggcaat cttctgaagc
     3181 tgtcggggcg cctggggaat ctggtggctc tccatgccgt tccaaccgaa gaccaggagg
     3241 aagtccgtct cctccgcatc gatttccgta tggacattct ggcggccgta tatccggccg
     3301 ttgacccaga agaggcccga aaactcctgg gtcagggggc tgtaatggta gcgtgagccc
     3361 aggccccgca tcaggcgaac gccgaaggct gcttcaaaat ggcatccctg tcccccgccg
     3421 cccatgtagg caaaggattt gggcccgtat tccccgacaa tttccttcag ctttgcggca
     3481 atttcgtcta tcgcctggtc ccaggagatc ctttcgaagc ggtcgcccac ccgtttcagc
     3541 gggtatttca gacggtcggc gttatgctgg tggttggcga tattcaatcc cttccggcag
     3601 acatatccca ggctgcggac gttgcccttg tctcccctta ccttgacgat gcggttgttc
     3661 tccacttcca cctccaggcc gcagttctgg gggcagagga cacatccggt ccggtgtact
     3721 gttcccatga tcattccttt ccttgcttgc tgattgatct ctttcagccg cggccggcct
     3781 gatcgcggat cttattcatc aggctgcgca gttccatctg ttcactctcc gtcaggaagc
     3841 cgagaaattc ccggttcgcc tcgactatca gccggaccgc caccccggcc aatttccttc
     3901 cgtgttccgt cagatggatc ctgatggacc gacgatcggc gggattgccg tggcgttcaa
     3961 tgagttgggc cgtttccagc cgatcgataa ttcccgtcat ggttgcgcta tccagttcag
     4021 cccgctttcc aagttcactg gaacttattc cgtcttcttc gttgagaaaa cccagcacca
     4081 gggcctggac cggtgtaatg ttcaattccg ctactttttg agccaaaaat cggctcccga
     4141 tctgattagc cttattgagg agaaagaata tgcaaaagtc cggtttcatg atttgctccc
     4201 gataatgctt gtctgcaata tacttgtgtg caagtataaa tgaaggatgc ggaattgtca
     4261 aggatcagcg gttaatgggt caagtggcaa aggggagaaa ctgatctgaa agaatggttt
     4321 tgcaaggggt gtggtaagat gaaggagcgg cggcgaaaaa aacaaagggc ggagggaagg
     4381 acgggtcggg ttttgtccta tggagcgtct gcattgtgcg aaaaatattc tgtgtcgcgg
     4441 aatttggacg ctgatcaatc tcccctttcc ttttgccggc aaatcggcta tacggcgttt
     4501 cttcaggcgg cattgaacaa ggaaaagaag tgaggatggt cgatatgaga ggcaaaaaga
     4561 ttacccttat cgtttggttg gcgttgttct tgttttttta cggcgccgtt gccggcgctg
     4621 cggcggcgac cccggcggaa gggtacaaaa aggccgagca gaagaccgag ccaaaggccg
     4681 agccggtgaa accgacggcc gacttgacgg tgagcgttct tagcaactac atctccaagg
     4741 gcgatgaact atcccggaac agcatcgtca ttcagcctga ggttattgtg ggctacaagg
     4801 gattttccct tgctatctgg ggaaacctgg atacccgtcc ctatctgtcc gagacgagca
     4861 cctacaaggg cgacaaacgg cagaactgga cggagaccga cttgaccctg gcctataacg
     4921 ggtccttcgg aatcgtaaat gccggtatgg cctatgccta tttcggcaac gccgccttct
     4981 ggaattccga cgccactggg gccgggccgg atatgaagga tgaccaggag gtctccctga
     5041 tcctgggcct gaatacgatc ctggccccga aactgaaggt gggccgctcc ttcgacagca
     5101 cccagatctg gtatctgtca ctgggcgtct ctcatgcctt tcagatcaac gaatacctcg
     5161 ggctgacttt agccgccacg ggcagctatc ttgtcagcca ggatgaaaac cgcctgaaga
     5221 tcaacagcaa cgggacggac cgcaagccgg acgggtctct gaacgaccgg tacaataatt
     5281 tccatgacgc cgtgatttcc ctgactctgc ctgtcaaacc gacggcgtat cttaccatta
     5341 caccccagct atcctacatc tttcccatgg ttgacgacgc aaaatacgat atgatgttcc
     5401 ggagcatgca gtgtgatacg accttttccg accgtcagag ctcctatctg gtggggggcg
     5461 tatcggcgac gttgagtttc tgagcccgga gcggggattc atttcagtaa gaggagactc
     5521 agggccatga ccagcattcc cgcgacgagc cccaagaggc tgtcgtggcc ctttccatag
     5581 gcccggctgg cgggcagcag ctcgtccagg ctgatgtaaa ccatgacgcc ggctataccg
     5641 ccgaacagga ggcccatgag ctggggcggg atcgcttcgc cttggccacc caggaagaaa
     5701 aggatcgcca gatagccgat gaccgctccg accggttcgg ccaggccgct ggccagggag
     5761 tatagaaagg ccttacggcg gttgcccgtg gcgtagtata tgggtacgga aacgctgatc
     5821 ccttccggga tgttgtgcag ggcgacggcc agcgcgatga cgattcccag cgccgtgtct
     5881 tccagggcgg cgagaaaagt gaccatgccc tcgggaaaat tatggatgcc gatggccaaa
     5941 gcggtgaaca gacccatgcg catgaggtta tactcggggc ggccgtggtt gtgaaagccg
     6001 gcagcggcct tttccggtcc ggacaaggcg ctggcggccg atgccggcgg cggcgccgcc
     6061 gggtcatgaa gaggccgtgc ttccgcttcg gcgtgggtct catggggatt cacggccgcc
     6121 ggaaccagat tgtcgatcag gccgatgatc agcatgccgc cgaagaacgc ggcggcgttg
     6181 aaccagggac cccggtcggc gccgtagtgc agtgagagga cggccaggcc ttcgggaaag
     6241 atctcgacga aggatacgta gagcatgatc ccggcggaga agcccgtggc cgcggagagg
     6301 aaacggtagt tcgtccgccc ggccgtgaag gcgatgacgc tccccacgcc cgtggccatc
     6361 ccggcaaaga cggtcagacc catcgctgtc cagaattcat tcatcccttg tccctatcct
     6421 tttgataaaa catgttttta cgttcgggat tttttggttt ggccaggtac agcccaatga
     6481 agatcagtgc cccacccagt aaatgatagg cgctgatccg ctctcccaga aatccgaagg
     6541 aaagaaaagc ggcaaatatt ggtagtaaat acaagaacat ggccgcagta ctcgggccga
     6601 cccggccgac gccgatattc cagcagaggt aggccaggat cgagggaaag aggcctaaaa
     6661 acagaaacac cggcagcacc cgtgaagtga aactgaaagt accggtaacg atgatttccc
     6721 cgatgtagaa cggggcgatg aacagcaacc ccagggcgat ggtcatggtc agcaggctta
     6781 agggatgcaa aacaatactg aatttgcgca gaaacactga atagagggac caactcgcca
     6841 cggacacgat caccagcagg tccccgtaat tgaagaccag attccggaca ttggccggag
     6901 agccttggaa gatgatccag agcatcccgg tcgtagagat gacgacgccc aggatctgga
     6961 agaccagcgg ccgttcatgg atgatcagcc aggccgcgac gatggtgatg gccggcatgg
     7021 tggccgtgac gatggacatg ttgttggccg tagtgaaatg ggcagcgaca tacaaaagag
     7081 aattgtaaac ggcgacgctg aaaaaggaga gcacgacaag cggcagtatg ttgcttttga
     7141 tcacgggcag atcccgcttt acggacagga atccgaaggg gaaaatcatg acgagggcga
     7201 agacccagcg ccagaaggac agggccatgg ggtgcacctc ctggatgaca ccccgggcca
     7261 ggacggtgtt ggcggcccag cacaggacgg cgaaggccag gccgg
//
LOCUS       DAOSMF010000090         7281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_11345_length_7281_cov_24.708829, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000090 DAOSMF010000000
VERSION     DAOSMF010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 7281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7281
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11345_length_7281_cov_24.708829"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1319)
                     /locus_tag="PLT63_08420"
     CDS             complement(<1..1319)
                     /locus_tag="PLT63_08420"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOD98786.1"
                     /translation="MNLGRMIEETANKYPGNMAVIHEKCRMTFAELNGAANALAHYLC
                     SLGIGRGDKVALMLPNCPEFIISYFAAQKLGAVAVTINVMSTPYELRHLLGNSDARVL
                     IAQSVAVRRYEEIKEDLPLCRHALITAGMDTDCPFTRVLRDHPRTWEMPAIDPDDPAV
                     MIYTSGLTGRPLGAQLTHANLMTQAVLLEEAYNGTTADRGLAVIPFFHSFGAAANMLT
                     AIRTGARIVLMERFTLESIFATIERERVTFTCSVPRMFLGMLFHQGGEKYDLSSLRLC
                     ITGGSTMPADFIPLFREKFGAPLVEGYGLTEASPICTITRLEQENKPGAIGTPIGGVE
                     ARIIADDNTDVPRGETGELIVRGKNVMKGYYKDERATAEVIRDGWLHTGDLGRMDADG
                     YIFLTGRKKRMVITSGFNVYPREVEIVLEMHPAVAAARVESKEDMMRG"
     gene            1580..2023
                     /locus_tag="PLT63_08425"
     CDS             1580..2023
                     /locus_tag="PLT63_08425"
                     /inference="COORDINATES: protein motif:HMM:NF021438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HOD98787.1"
                     /translation="MKKTMLMAGVIFFFSVSLALAAGSPVGKWKTIDDETKKEKSIVE
                     VYEVNGKIYGKIVQLLQEKDGGAAKLCTKCPGGDKNKPMLGLVFLKDLKADGSEYTGG
                     TIMDPNNGKIYKCKLEVVEGGGKMKVRGFLGVSLLGRTQIWHKVK"
     gene            2245..3102
                     /gene="folD"
                     /locus_tag="PLT63_08430"
     CDS             2245..3102
                     /gene="folD"
                     /locus_tag="PLT63_08430"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041584935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HOD98788.1"
                     /translation="MPEIINGNEIARVIREEIRKETVVMREITGVTPGLAVVLVGDDP
                     ASRIYVRRKGRACAEAGFLSREIRLPAAAAEGELLDIIVQLNGDPAVHGILVQLPLPR
                     QIRTQMIIEAIAPAKDVDGFHPVNVGRLMAGNPGPVSCTPRGVMELLDRTGVDIAGRE
                     AVIVGRSNIVGKPLALLLLARHATVTICHTRTQRLPDVTRRADILVAAAGSPEMIRGD
                     MIKEGAVVIDVGINRRDDGRLVGDVAFAEAERRAAYITPVPGGVGPMTIAMLLRNTLE
                     AARRITAPV"
     gene            complement(3208..4359)
                     /locus_tag="PLT63_08435"
     CDS             complement(3208..4359)
                     /locus_tag="PLT63_08435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOD98789.1"
                     /translation="MDPLKRYRQRLRIWSNDQRLEAERLHYEGLFASRRLSVPDEEAL
                     RAACRERFPSLRPKPRGALKILAVYHDYNWEETALKPALQKFGTVYHFDWFRGPGAAA
                     GEWRRSGRPLMNRELLAYARKLNRGRDLDVIFTYLSGEQITPAAMASLNALGVPIINL
                     ALNDKENFVGKIRGGQALGVRDICRFFTLCWTSTEDARKKYVVEGALPIYLPEGANPE
                     LHRPREGEKTIDVSFVGQRYGNRPEVVARLAAAGIKAVVRGPGWPDGPLSTEDMVRLY
                     SQSRINLGFGGVSGHSDAFCLKGRDFEIPMSGGLYLTEHHPELERVYEIGWEIVTYRD
                     FDDLLVKIRRLLSNPALAEEIRRRGRERALRDHSWESRFQKVFAISGVI"
     gene            complement(4542..5471)
                     /locus_tag="PLT63_08440"
     CDS             complement(4542..5471)
                     /locus_tag="PLT63_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98790.1"
                     /translation="MVKIPAAEMGLKTISGDFSFQRLEIDGIDNRAVLTANKPEMAAE
                     EICLFHPDLADGNACLQSLQERVEAAMAHRAPFPVVRFADGEYAFYARNLECNGLYRQ
                     AESRAAIRAALPFHVEALTRLSRTGLLAPLVFPGNIAPPRRGIFSLFSGFRSRPSAVT
                     FLDFLHRHDVSLTGGNYLPFYAVYAWLSSPAFTRLTDGKRVCLIGSDWDEQACRDWFA
                     RGDSGPRLSFVKIPAEFVATRWPEMKAAVVGTVPADTELCLTGAGIGALPVCVDVAAS
                     LSIPVLDAGHVFNMMNDRIDKSNGDRLYTRYRR"
     gene            complement(5465..5836)
                     /locus_tag="PLT63_08445"
     CDS             complement(5465..5836)
                     /locus_tag="PLT63_08445"
                     /inference="COORDINATES: protein motif:HMM:NF039992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SxtJ family membrane protein"
                     /protein_id="HOD98791.1"
                     /translation="MELDRLGTVKTVNILTLAVLIAYLIFGAVWLVWVAALLALGNAL
                     ESRVTSALARGWLKFAERLGAFNSRVILTLTFYLILTPLAFLYRIFNRHLVEHFLANK
                     RKSYFEDLNKKYAPGDFEKSW"
     gene            complement(5915..6061)
                     /locus_tag="PLT63_08450"
     CDS             complement(5915..6061)
                     /locus_tag="PLT63_08450"
                     /inference="COORDINATES: protein motif:HMM:NF039953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HOD98792.1"
                     /translation="MEILKDFWGFLKERKKWWLLPMIIMLLLMGLLIMSSGSAVAPFI
                     YTLF"
     gene            complement(6242..>7281)
                     /locus_tag="PLT63_08455"
     CDS             complement(6242..>7281)
                     /locus_tag="PLT63_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98793.1"
                     /translation="KLKKAAKLIIVNIVLLLIVLSLLEGMLLLLVRNPDILKHCPRRI
                     GNMIGYLYARERPTIQFEAECARHDPVLGYTLKPGSCTFGGREFTNRYDINSLGVRDG
                     EAALGHPEIIVAGDSFAMGWGVEQDETFAALLQHRTGLKVLNAAVSSYGTVRELGILR
                     SVPRDRLKYLIIQYCGNDREENREFYLQNGRLPVMTAERYEEYREIYQESQRYYPGKY
                     LGMKIGKRWEEMEQKRRQEQAPKLDRDEIDLFLYALQNSGLDLEKATLIVFVMNGRNP
                     NDNREFPAALKRKLAAGDYPSYLKDMLVLDFSSRLGPEHFYVLDDHLNATGHAVVAAE
                     LAQVIAGRGRP"
BASE COUNT         1404 a         2118 c         2328 g         1431 t
ORIGIN      
        1 ccccgcatca tgtcctcctt gctttccacc cgggccgccg ccacggcggg gtgcatctcc
       61 aggacgatct ccacctcccg gggatagacg ttgaagccgc tggtgatgac cattctcttt
      121 tttctgcccg tgaggaagat atatccatcc gcgtccatcc tccccaggtc gccggtatgg
      181 agccagccgt cccggatgac ctccgcggtg gcccgctcgt ccttgtagta gcccttcatg
      241 acgttctttc cccggacgat cagctcgccc gtttcccccc ggggcacgtc agtattgtcg
      301 tcggcgatga tccgggcctc gacgccgcca atgggcgtgc cgatggcgcc gggtttgttt
      361 tcctgctcca gccgggtgat ggtgcagatg ggcgaggcct ccgtcaggcc gtaaccttcc
      421 accagcggcg caccgaattt ttcccggaag agggggatga aatccgccgg catggtggaa
      481 ccgccggtga tgcataaccg cagggaactc aggtcatact tttccccgcc ctggtgaaag
      541 agcatcccga gaaacatccg ggggacggag caggtgaagg tgacccgttc cctttcaatg
      601 gtggcgaaga tgctctccag ggtgaagcgc tccatgagaa ctatgcgggc cccggtgcgg
      661 atcgcggtga gcatattcgc cgccgctccg aaggagtgga agaagggaat caccgccagc
      721 ccgcggtcgg cggtcgttcc gttatacgcc tcttccagga gcaccgcctg ggtcatcaga
      781 ttggcgtggg tcaactgcgc ccccagcggg cgtccggtga ggccggaggt gtaaatcatt
      841 accgccggat cgtcggggtc tatcgccggc atctcccagg tccgggggtg gtcgcggagg
      901 acccgggtga agggacagtc cgtatccatg ccggcggtga tcagggcgtg ccggcagagg
      961 ggcagatctt cctttatttc ctcgtaacgc cggacggcga cgctctgggc gatcaagacc
     1021 ctggcgtcgc tgttgcccag aagatgtcgc agttcgtagg gagtggacat gacattgatg
     1081 gttacggcca cggcgcccag cttctgggcc gcgaagtagg agatgatgaa ttccggacag
     1141 ttgggaagca tcagggcgac cttgtcaccc cggccgatcc ccaggctgca caggtaatga
     1201 gccagggcgt tggcggcgcc gttgagctcc gcaaacgtca tccggcattt ttcatggatg
     1261 acggccatat tgccgggata tttgttcgcg gtctcctcta tcatccttcc caggttcata
     1321 attgtctctg cgcccccctt tatcattgtt gattgcggat cggactccgg atcggcaaat
     1381 gcccgcgccc cggccggtca tcaggccggc gaacccggcg tccggagatc ttcccgccac
     1441 ttttcgcgac ttttgcttag cacatgcctc cggccttttc aatgctggag atggtctttt
     1501 ttcttgatca cggcggggat ttcgtatagg aatgaaccgc ctgcgggcgt gctcaataaa
     1561 cttcaaggag gttgcagcga tgaaaaaaac gatgttgatg gccggggtga ttttcttctt
     1621 ttccgtttct ctggccctgg ccgccgggtc gccggtgggg aagtggaaga ccatcgacga
     1681 tgaaacgaag aaagaaaaat cgattgtcga ggtttacgag gtaaacggca agatctatgg
     1741 caagatcgtc caattgctcc aggaaaagga tggcggggcg gcgaagctgt gcacgaagtg
     1801 tccgggaggc gacaaaaaca agcccatgct gggcctggtc ttcctgaagg atctcaaggc
     1861 ggacggcagt gaatacaccg gcggcaccat catggatccc aacaacggca agatttacaa
     1921 gtgcaagctg gaggtcgtgg aagggggcgg caagatgaag gtccggggtt ttctcggggt
     1981 gtcgctgctg ggacgcaccc agatctggca taaggtgaaa tgagtgatat tgaacaatat
     2041 actaaggttg ccgacatcgt aaaaggtcaa aaaatgtccc atttgggacg gcatcttaaa
     2101 aagatccgca ggcgaggcgc gcgaatcctg aggaatgagg cgtactggga cgtacgtcgc
     2161 agtgacgagg gatgaagcgc aacgaagcat ccggactttt tacgatgccg tcaaggttgc
     2221 atagtaaata tacgcaagat tcttatgccc gagattatta acggcaatga aatcgcccgg
     2281 gtgatccggg aggagatccg taaggaaacc gtagtgatgc gggagataac gggggtgact
     2341 cccggtctgg ccgtggtcct tgtcggcgat gatccggcgt cgcggatcta tgtccggcgc
     2401 aagggccggg cctgcgcgga ggcgggcttc ctgtcccggg agatacgcct gccggcggcg
     2461 gcggcggaag gggagctttt ggatattatc gtgcaactga acggggatcc cgccgttcac
     2521 ggcatcctcg ttcagttgcc gctaccccgc cagatccgca cccagatgat catcgaggcc
     2581 atcgctccgg ccaaggacgt ggacggcttc catcccgtga acgtgggccg cctcatggcc
     2641 ggaaatcccg ggccggtgtc ctgcacgccc cggggcgtca tggaacttct ggaccgcacc
     2701 ggcgttgaca tcgccgggcg ggaggcggtc atcgtcggcc ggagcaacat cgtcgggaaa
     2761 cccctggccc tgctcctgct cgcccgtcat gccacggtca ccatttgtca tacccggacg
     2821 cagcggctgc ccgatgttac ccggcgggcc gatatcctgg tcgcggcggc ggggagcccc
     2881 gagatgatcc ggggggacat gatcaaggag ggggccgtgg tcatcgacgt ggggatcaac
     2941 cgccgggatg acggccggct ggtgggggat gtggccttcg cggaggcgga acgccgggcc
     3001 gcctatatca cccccgtacc cggcggcgtc ggtcccatga ccattgccat gctcctgcgc
     3061 aacaccctcg aggcggccag gcggatcacc gctccggttt gaagcgccaa taatataagg
     3121 tgaacccgca aaaagcgatg gcgcaggaaa tgccaatatt tgctcccctt atctctttcg
     3181 gagcgcggtt ccgcgtccgg gggcgaatca tattacgccg ctgatcgcga ataccttttg
     3241 gaagcggctc tcccaggaat ggtcccggag cgcccgttcc cgcccccggc gccggatttc
     3301 ctccgccaac gcggggttgg aaaggagccg gcggatcttg accagcaggt cgtcgaaatc
     3361 ccggtaagtc actatttccc agccgatttc ataaaccctt tccagttccg gatgatgttc
     3421 cgtgaggtag agaccgccgc tcatggggat ctcgaagtcc cggcccttga ggcagaaggc
     3481 gtccgaatgg ccgctgacgc cgccgaagcc gagattgatc cggctttggg aataaaggcg
     3541 caccatgtct tccgtgctca gggggccgtc gggccatccc ggtccccgga cgacggcttt
     3601 gatccccgcg gcggccagcc gggcgacgac ttccggacga ttgccgtagc gctgccccac
     3661 gaaggagacg tcgatggtct tttccccctc ccggggacgg tgcagctccg ggttggcccc
     3721 ttcgggcagg tagatgggca aggccccttc cacgacgtat tttttccggg catcctccgt
     3781 gctggtccag caaagggtga agaagcgaca gatgtcccgg acacccaggg cctggccgcc
     3841 ccggatcttg ccgacgaaat tctccttgtc gttcagggcg aggttgatga tcggcacgcc
     3901 cagggcgttc agggaggcca tggcggcggg cgtgatctgc tcgccggaaa gataggtgaa
     3961 gatgacatcg agatcccggc cgcggttcag ctttcgggca taggccaata gttcccggtt
     4021 catcagggga cggccggaac ggcgccattc accggccgcg gcgccgggac cgcggaacca
     4081 gtcgaaatga taaacggtgc cgaatttctg caaggccggc ttcaaggccg tttcttccca
     4141 gttgtaatcg tggtaaacgg ccaagatctt caacgccccc cggggcttgg ggcggaggga
     4201 gggaaagcgc tcccggcagg cggcgcggag ggcttcctca tcgggaacgc tcaggcgccg
     4261 ggaggcaaaa agcccttcat aatgaagacg ttccgcttcc aggcgctggt cgttgctcca
     4321 tatccggaga cgctgtcgat atctcttcaa cggatccatg gggagggcct tctcgatggt
     4381 gttcctgaac tcggttgaat cctctcgggg tatattaccc gaagcgtgct tcgttccttg
     4441 aggttttaat gcctcgcgtc ctgccgcgag gagattcatt gggttgaccg ccggcgctcc
     4501 gggcgaaccg tcgtccccgc gggtaacaaa gccgccggcg cttaccggcg ataacgggtg
     4561 taaagccggt ccccgttcga cttgtcgatc cggtcgttca tcatgttgaa gacatgcccc
     4621 gcgtccagaa cggggatgga aagggacgcg gcgacgtcaa cacagacggg aagggccccg
     4681 atgcccgccc cggtgagaca gagctccgtg tcggcgggta ccgtcccaac gacggccgct
     4741 ttcatttccg gccaccgggt ggccacgaat tcggcgggaa tcttgacgaa actgagccgt
     4801 ggcccgctgt cgccgcgggc gaaccagtcc cggcaggcct gctcgtccca gtccgaaccg
     4861 atgagacaga ctctcttccc gtccgtcaag cgggtgaaag cgggagagga aagccaggca
     4921 tagacggcgt agaagggaag atagttgccg ccggtaaggg agacgtcgtg gcggtgcaga
     4981 aaatcgagaa aggtaacggc cgacggacga ctccgaaaac ccgaaaacag ggagaaaatc
     5041 ccccggcggg gcggggcgat gttgccggga aacaccaggg gggccagcag gccggtccgg
     5101 gagaggcggg tgagggcttc gacatggaag ggcagggccg ccctgatggc cgcccgggat
     5161 tccgcctgcc ggtaaagacc gttgcattcc aggttccggg cgtaaaacgc gtattcgccg
     5221 tcggcgaaac ggacgacggg gaagggggcg cggtgggcca tcgcggcctc cacgcgttcc
     5281 tgaaggcttt gcagacatgc gtttccgtcc gccaggtcgg ggtgaaaaag gcagatttcc
     5341 tcggcggcca tctccggctt gttggccgtc agcacggcgc ggttgtcgat cccgtcgatt
     5401 tccaggcgct gaaaagagaa atctccggag atcgtcttaa gccccatctc tgccgccggg
     5461 atctttacca tgacttctcg aagtccccgg gggcgtattt tttgttcagg tcttcaaaat
     5521 aactcttacg cttgttggcc aggaaatgct ccacgaggtg gcggttgaaa atccggtaca
     5581 ggaaggccag gggggtgagg atcagataaa aagtcagcgt caggatgacc cgggagttga
     5641 aggccccgag ccgctcggcg aacttaagcc agccccgggc cagggctgag gtgacccggc
     5701 tttccagggc gttgcccagg gccagcaggg ccgccaccca taccagccag acggcgccga
     5761 agatcaaata ggcgatcagg acggccaggg tcagtatgtt taccgttttg acggttccta
     5821 aacgatcgag ttccactttc ttcgtctctt cccctccttg ccccgcggga acgacccgga
     5881 gaatcagaag gagatcgcct aaccccaacg ggggttagaa cagggtgtag ataaagggcg
     5941 ccacggcgga gccgctggac atgatcagga ggcccatcag cagcagcatg atgatcatag
     6001 gcaggagcca ccattttttc cgttctttca aaaagcccca aaaatctttc agaatctcca
     6061 tatcggcacg tctttcctat ccattgcttt tcgttggctt gaaggttggg cgtcaagacc
     6121 cggcctccca cggcgggcgg gcccggatgg aatgacggcg cccccctcat atcgcgaaag
     6181 ccttttcgtt tcggcctgtt cgtatagcaa tgagtttttg attatgcaag ggaaaataat
     6241 ttcagggccg cccccggccg gcgatgacct gggccaattc ggcggcaacg acggcgtgac
     6301 cggtggcgtt gagatggtcg tccaggacat agaagtgttc cggacccaac cgagaggaga
     6361 aatcgaggac cagcatgtct ttcaaataag acgggtagtc gcccgcggcc agctttcttt
     6421 tcagcgccgc cggaaactcc cggttgtcgt tgggattacg gccgttcatg acgaagacga
     6481 tgagcgtcgc tttctcgagg tccaggccgc tgttttgcag ggcgtagaga aacaggtcga
     6541 tctcgtcccg atcgagcttc ggcgcctgct cctgccgtcg tttctgttcc atctcctccc
     6601 agcgtttccc gatcttcatc cccaagtatt tccccgggta gtaccgctgt gactcctggt
     6661 aaatctctct gtattcttca tatctttctg ccgtcatgac ggggaggcgc ccgttctgga
     6721 ggtaaaactc ccggttctcc tcccggtcgt tgccgcagta ttgaatgatg aggtatttca
     6781 ggcggtccct ggggacgctc cggaggatcc ccagttcccg aaccgtcccg taagaggaaa
     6841 cggcggcatt gaggaccttg agacccgtcc ggtgctgcag gagggcggcg aaggtttcgt
     6901 cctgttccac cccccagccc atggcgaagg agtcgccggc gacgatgatc tcgggatgcc
     6961 cgagggccgc ctcgccgtcc cggacgccca ggctgttgat gtcatagcga ttggtgaatt
     7021 cccgtccgcc gaaggtgcag gacccgggct tgagggtgta gcccaggact gggtcatgac
     7081 gggcgcattc cgcctcgaac tggatcgtgg ggcgctcccg ggcgtagagg tagccgatca
     7141 tgttgccgat ccgccggggg cagtgtttga ggatgtccgg gttgcggacg agaaggagca
     7201 gcatcccctc caggagagac aggacgatca ggagcaggac gatattgacg atgatcaact
     7261 tggcggcttt tttcaacttt g
//
LOCUS       DAOSMF010000091         7112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_11682_length_7112_cov_22.842851, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000091 DAOSMF010000000
VERSION     DAOSMF010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 7112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7112
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11682_length_7112_cov_22.842851"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            359..895
                     /locus_tag="PLT63_08460"
     CDS             359..895
                     /locus_tag="PLT63_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98794.1"
                     /translation="MTGGEDIDFSGLEIDLILEDGMERELGSWLTCWVIATPGHTRDS
                     LSFSLPELEAMIMGEAVGVFDRNFKIHHEFTSCYRNYLFSFYILSFLSIEILMMSYFF
                     TLTGVNAREYVHKSLVATREFKARIERYLTDCHGNCQEVAERIFQEVFTATGAILQDA
                     RPYRTNLEAKIRAIAEGR"
     gene            complement(1436..1675)
                     /locus_tag="PLT63_08465"
     CDS             complement(1436..1675)
                     /locus_tag="PLT63_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98795.1"
                     /translation="MKKLLVIVMVLMISGCAAAIKESELGKHDSIYKDMSHLWFSWVG
                     YRDVDQNDVNKSTARGWWGITTPYRPDDAKGSGKP"
     gene            complement(1732..1908)
                     /locus_tag="PLT63_08470"
     CDS             complement(1732..1908)
                     /locus_tag="PLT63_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98796.1"
                     /translation="MATIMPDSDDVQRAIKWISQALEEKPDQSVGKMVEQAVFKFDLS
                     PVDSEFLIGFFKKR"
     gene            complement(1926..4535)
                     /locus_tag="PLT63_08475"
     CDS             complement(1926..4535)
                     /locus_tag="PLT63_08475"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOD98797.1"
                     /translation="MNIKRLLAKSSLESRSLYYRLTIIFSLFFLFPLLGFFFFAFKYN
                     ILHDEYIPIFFISLLFFYLFGYSLVRRIFDTIVSVSKKMAVSMAESGKVVTSDEVNEL
                     EEIVTSFQMLEKDLRSTFADLRQKSAQITTLKELSDLCYVTFDTEDLFYITLERALKI
                     VNADVGSVLLLENPDRETFLVQASIGLGGIIKKGDRIDFTTSVAKFAVINKSPLLVND
                     IETDSRFGRRNREQYGTKSFLCMPLKGINEVIGVMTMSRRASDSCFTQEDADILTPLL
                     SNAAFTYDNLALMKENESQRRLVKVMDMGFKMIGAKSMDLDLVHTYLYQMRDAVIYDL
                     AVIMLVRKDENATVKIFDYLAYIPTDLVRNKIYPYRGTVLEKVMQQGSALVIKNPAEL
                     GTSDVERDLNDKHQLKSICLAPLQSQGEPAGVLFLGSQQASFFHEKKDHVDLAASLIS
                     LAIDRGRLTSLVEKRDREMDFLKQIGSLLASSTFDMDEVIKHTMQMIQVMINVEAGSL
                     LLREDDQLVFKESFNTNKEVNLSALKNLKLLLGKGIAGYAATRGEAVIVNDVKTSQYF
                     YPLLDEKTGFVTKSVLSVPLVSKGRVLGVIEVLNKRRGEFNAGDMQLLQSIGTSVSIA
                     LENASLYRETLAMAEQERCIRGVFQKFVPKEVVDRIVHNVQMDQQVAEELRMLTMLNI
                     DLRDFSRLSMKIGPRKTVAMLNLFFHVMGEIIFKHRGIVDKYLGDGFLAVFGAPVSYP
                     QDADNAIAAALEMQQSMKELSCRAIELINMPLSMGISIHTGEAVVGNIGFEKKMDYSV
                     IGDSVNIVFRLQGLTKRRANSILITEKTLQSAIGSVVEVKSIEIVDDPSLISNLAVYE
                     VIGQQQRDKLSTN"
     gene            4745..5185
                     /gene="rpsF"
                     /locus_tag="PLT63_08480"
     CDS             4745..5185
                     /gene="rpsF"
                     /locus_tag="PLT63_08480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOD98798.1"
                     /translation="MTRRYETIFITPADIPDEELQTLIDRCAAIITGRKGLLVKVEKW
                     GKRKLAYEIKKQLRGFYVLLDFAGQTDIVNELERNFKIDDKILKYMTVKKDEVVDLQA
                     LEGELSAAAPEAKPLESAAPAASEEKEAAGNDSTLAVTPTSSGE"
     gene            5200..5463
                     /gene="rpsR"
                     /locus_tag="PLT63_08485"
     CDS             5200..5463
                     /gene="rpsR"
                     /locus_tag="PLT63_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOD98799.1"
                     /translation="MTPVRSPRPQRPQKKFFHRRKFCRFCTDSNLKIDYKDPHTLRDF
                     VTERGKIMPRRITGNCSKHQRALTLAVKRARMIALLPFAVSES"
     gene            5542..6522
                     /locus_tag="PLT63_08490"
     CDS             5542..6522
                     /locus_tag="PLT63_08490"
                     /inference="COORDINATES: protein motif:HMM:NF021506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HOD98800.1"
                     /translation="MTGEEGRNSLMIRGIFRGTLTTSLFFLTVALVPFLSSMLLIFIP
                     FPVCFSFAKLGRTGGLAVLIFSLLILVVVQHTIASQVSMTAFIILGGLGMIISEFLKR
                     NLTVGWTVSLAAATGTLIMGTMILASSMMHGKTPGDHIYHYVQAMVAENIKLYGELDL
                     PAEQLKFLKDNSTRIARVLKYTFPALSLIGISIIVLINVLVSRAVFRLRAIPFPDFGN
                     LTRWKAVEHLVWLFILSGIGLLIPLAEIKFLSLNILAICLFVYFLQGLAIGEFFFHKR
                     KAPFFLRFLFYFLIMIQQYLMLLIAAAGLFDLWLDFRKLSTPAPKVNKNP"
     gene            6543..6989
                     /gene="rplI"
                     /locus_tag="PLT63_08495"
     CDS             6543..6989
                     /gene="rplI"
                     /locus_tag="PLT63_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOD98801.1"
                     /translation="MKVILKENLEDLGKIGDIVNVSDGFARNYLIPKGFAAEATSKSI
                     QSLEHERKRILQKAEKEKQQAAALVEKLAGFTCVIARRVGEQDKLFGSVNNKDIEKAL
                     KEMGLEIDRKTIVMSEPIKTLGEFPVKIKLPAGLAAEINIQVVKEG"
BASE COUNT         1796 a         1846 c         1689 g         1781 t
ORIGIN      
        1 tttgcccagc tggtttcata atcatcggga aagaatttcc cgcggttcaa ttccccctgc
       61 cagggaggta acaacatgaa cctaagagca tcaagctttg cccacgtgat caaaaatcaa
      121 gtcatgatcc agactatatc gacgaattag aaaagatttt caccgacagc aaccatctgc
      181 gctgccactt tctcacccat tcctacttcg accatgccgg cgcctctccc tgtctgaaga
      241 ggcttatccc caaactgaag atcggcgctg ctctggcggc agcggaaaca ttccgtaaat
      301 ccagtgccgt ggaactgatc cgcgaactca gcgccccctg ggaagagaga tacaaatttt
      361 gactgggggc gaggacattg acttttccgg tctggaaatc gatctgattc ttgaagacgg
      421 catggagcgg gaactggggt cgtggctgac ctgctgggtt atcgctaccc ctggacacac
      481 ccgtgattcc ttgtcttttt ccctccccga gttggaagcc atgattatgg gcgaggcggt
      541 cggcgttttc gaccgcaatt tcaaaattca tcacgaattc acttcatgct atcgtaatta
      601 cctgttttca ttctatattt tatctttcct atcaatagaa atactgatga tgagttattt
      661 tttcacgctc accggcgtca atgcccgtga atatgtgcat aaatccctgg tggcaacccg
      721 ggaattcaaa gcaaggattg aacgttacct tactgattgc catggaaatt gccaggaggt
      781 ggcggaaagg atctttcagg aggtttttac agccaccggg gccattctcc aggatgcccg
      841 cccctaccgt accaatctgg aggcgaaaat tcgggccata gccgaaggaa gataatctgt
      901 ccctccacta ttcacccagg accggagata ttgccgatcc taccatcttt caataggttc
      961 agtaatatat aaatataccc gtgactggct gttacccgac gagagcatcg ttttgcataa
     1021 ataagtctag cattgggaca acgataataa gttaaaaaga taatatacta ccttttcatg
     1081 ctcgtcaaag ttcacggaac atgaggtatt tcttgataca gcatcaagct cgatgactcc
     1141 gtaaaagtcc agcaagcaaa gcctaacgtt cctaaaccgg acatctgcca cagatttagg
     1201 caaggcacgc tcataatgag ggacaatgag cgcttatgct taagccgtcg tgaccagaga
     1261 tgtaacgttt tgccacagtc ggactgtata cgaagccatc aagcttgaca gcggttatct
     1321 gcccggaaat caccgcggca gactgcgatc cccgggttaa gatacatcgg aacggacacc
     1381 tgtcgcccat tccgtttccg ccccactttt caactaatct ctgcggtaaa ggatattaag
     1441 gctttcccga tcctttcgca tcatcgggtc gatatggggt ggtgataccc caccatccgc
     1501 gggccgtcga tttgttcaca tcgttttgat ccacatcccg ataaccaacc caactgaacc
     1561 atagatgact catatctttg taaatagaat catgcttccc caattcggat tctttgatcg
     1621 ccgcggcgca gccggaaatc ataagtacca tgacaattac caacagtttt ttcatcgctt
     1681 atcctccttt gaagttttta ccgtttatat ggaacggtgg ttcatcatca tttaacgttt
     1741 cttgaaaaaa ccgatcagga attcactgtc caccggagaa agatcgaatt tgaaaaccgc
     1801 ctgctccacc atcttcccaa cggattgatc cggcttttct tccagagcct gagaaatcca
     1861 cttgatcgcc ctttgtacgt catcgctatc cggcatgatc gtagccataa agttctacct
     1921 ccttcttaat tagttgataa cttatccctt tgttgctggc cgatgacctc gtaaacggcc
     1981 agattgctga tcagcgaagg atcatcgaca atttcaatac tcttcacttc caccacggaa
     2041 ccgattgccg actgcagggt cttctccgtt atcagaatcg aattcgctct ccgcttcgta
     2101 agcccctgaa gacggaagac gatattcacg gaatccccga tcaccgagta gtccatcttc
     2161 ttttcgaagc cgatattgcc caccaccgcc tcgccggtat ggatgctgat gcccatgctc
     2221 aggggcatgt taatcaactc tatcgcccgg caactgagct ctttcatgga ttgctgcatc
     2281 tccaaggcgg cggcgatggc gttatcggca tcctggggat aggaaacggg ggcaccgaaa
     2341 acggccagga atccatcacc aagatattta tcgacgattc cccggtgttt gaagattatc
     2401 tcacccatga catggaaaaa aagattgagc atggccaccg ttttccgcgg tccgattttc
     2461 atagatagac gggaaaaatc acgcagatcg atattgagca tcgtcagcat gcgcagctcc
     2521 tcggcaacct gttgatccat ctggacgttg tgcacgatcc tgtccactac ttccttgggc
     2581 acgaatttct ggaacacccc ccggatgcat cgttcctgct ccgccatggc gagggtctcc
     2641 cgatacagac tggcattttc caaagctata ctcaccgagg tgcctatgga ctggagaagt
     2701 tgcatgtctc cagcgttgaa ttctccccgt cgtttgttga gtacctcgat cacccccagg
     2761 accctcccct tggatacgag gggtacagaa aggacggatt tcgtcacaaa accggtcttt
     2821 tcgtccagca ggggatagaa atattgggaa gttttcacat cattgactat taccgcctct
     2881 ccccgcgtcg ccgcataacc ggcaatgcct tttccgagca gcagcttgag attcttcagt
     2941 gctgacagat ttacctcctt attggtattg aaggattcct tgaacacgag ttgatcatct
     3001 tcccgcaaca gcaacgaacc cgcctcgacg ttgatcatca cctggatcat ctgcatggtg
     3061 tgcttgatga cttcatccat gtcaaaggtc gaggaagcga ggaggctgcc gatctgcttg
     3121 agaaaatcca tctcccgatc cctcttttcg accagggaag tcaggcgccc ccgatctatg
     3181 gccagcgata tgaggctagc tgccagatcc acgtgatctt tcttttcgtg gaaaaaggag
     3241 gcctgctggg aacccaagaa aaggactccc gccggttcac cctggctctg caacggcgcc
     3301 aggcagattg atttgagctg atgtttgtca ttaagatccc gctcgacgtc acttgtcccc
     3361 aattccgccg gattcttgat aaccagggcg ctcccctgtt gcatgacctt ttccaggacg
     3421 gtgcctcggt aaggataaat cttgttcctg accaaatccg tcggaatgta tgccagataa
     3481 tcgaagatct tcaccgtggc gttttcgtct ttcctaacca acatgatgac ggccagatcg
     3541 taaatgacgg cgtcccgcat ctgatacagg taggtatgga caaggtcgag atccatgctt
     3601 ttagctccga tcatcttgaa gcccatatcc atgaccttga ccaagcggcg ctgtgattcg
     3661 ttctccttca tcagggccag attgtcatag gtaaacgccg cattgctcaa caggggcgtg
     3721 aggatatcgg catcttcctg tgtgaagcag ctatccgagg cacgccgcga catagtcatg
     3781 accccgatga cttcgttaat tcccttcaga ggcatgcata ggaaggattt cgtcccgtac
     3841 tgctcccggt ttctgcgacc gaaacggctg tcggtttcga tatcattcac cagcaacggt
     3901 gatttgttga taacagcgaa tttggcaaca ctcgtcgtga aatcaattcg atctcccttt
     3961 tttattatac cgcccaggcc aatgcttgcc tggacaagga aggtctctcg atcaggattt
     4021 tccaggagca ataccgaacc tacatcggca ttgacgattt tgagggcccg ctccagggta
     4081 atatagaaga gatcttccgt gtcgaaggta acataacaga gatcagatag ctccttgagg
     4141 gtcgttatct gagcggattt ttgtctcaaa tcggcaaagg tagaccgcag atctttctcc
     4201 aacatctgga aggaggtcac gatctcttcc agttcattga cttcatcgct tgtgacgacc
     4261 tttccggatt cggccatgga gaccgccatc tttttggaaa cggagacaat cgtatcgaaa
     4321 atccggcgca cgagggagta gccaaaaaga taaaagaaca acaggctgat gaagaaaatg
     4381 ggaatatatt catcatgaag gatgttgtac ttgaaagcga aaaagaaaaa acccaggagg
     4441 ggaaagagaa aaaacagact gaagattatc gttagtcggt agtaaagact tctgctctcg
     4501 agagatgatt tggcaagcag tctttttatg ttcatcttgt tccctagtat atccaaatga
     4561 gatcggcttt cctatttacc tgaatgcgtt ttgttttaca agttcattca taacttggtt
     4621 tggatgcgca caaagtcctt gctttccaaa acggcttgat gtaataagtg cgcactttta
     4681 taatcattgc cggaactcct tgttcaggcc cggagcctgg actttagagc cgagaggagg
     4741 aaagatgacg agaagatacg aaaccatctt cattaccccc gctgacattc ctgatgagga
     4801 actgcagacc ttgatagata ggtgcgctgc gatcattacc ggtcgtaaag gcctgctggt
     4861 caaggtggaa aaatggggca agcgtaagct ggcctatgaa atcaagaagc agcttcgtgg
     4921 tttttacgtg ctgctggatt tcgctggcca aacggacatt gtcaatgaac tggaaagaaa
     4981 tttcaagatt gacgacaaga ttctcaaata catgactgtg aagaaagacg aggttgtcga
     5041 tctccaggcc ctggaaggag aattgagcgc cgccgccccg gaagccaaac cattggagag
     5101 tgctgccccg gccgcatctg aggaaaaaga ggccgccgga aatgattcca cgttagcagt
     5161 aacgccaacg tcatcaggag aataagtgga gaagaggaaa tgacacctgt aagaagccca
     5221 aggccgcaac ggccgcaaaa gaagtttttt caccggcgaa aattctgccg gttctgtacg
     5281 gattccaatc tgaagataga ctacaaggat ccccacacat tgcgggactt cgtcaccgag
     5341 cggggaaaga tcatgccgag aaggataacg gggaattgct ccaaacacca gcgtgccctg
     5401 actctggccg taaagcgggc ccggatgata gccctgcttc cctttgccgt atccgaaagc
     5461 tgatattcac caggttcggc tgtcgcccgg ccaattcgcc ccctcgggcg tcccggccgg
     5521 tatggagatg gttaaacggg catgaccggg gaggaaggaa gaaacagcct catgatccgg
     5581 gggatcttcc gaggtacatt aactacctcg ctcttctttc taaccgtggc gctggtacct
     5641 tttctcagtt ccatgctgct gattttcatt ccgttcccgg tatgtttctc ctttgcaaag
     5701 ctcggccgga ccggcggcct ggccgtatta atcttttccc tgcttatcct cgtcgtggtg
     5761 cagcacacca ttgcttcgca ggtatctatg actgcgttta tcatcctggg aggtctgggg
     5821 atgatcatct cggaattctt gaagaggaac ctcaccgtgg ggtggacggt ttccctagcg
     5881 gccgccaccg ggactctcat catgggtaca atgatccttg caagcagcat gatgcacgga
     5941 aagactcctg gggatcatat ctatcattat gtgcaggcaa tggtggcgga aaacatcaaa
     6001 ttgtatggcg agctcgacct gcccgccgaa cagttgaagt tcctgaaaga taattccact
     6061 cggatagcca gggttctaaa gtatactttt ccggccctgt cccttatcgg tatctccatc
     6121 atcgtcctga tcaacgtcct tgtatctcga gcagttttca ggctccgagc gatccccttc
     6181 ccggacttcg gcaacctgac ccgctggaag gccgtggagc acctcgtctg gctatttatc
     6241 ctatccggaa tcggtcttct gatccccctg gcggagatca aattcctgtc cctgaatatc
     6301 ctcgccatct gcttgtttgt ttatttcctt cagggtctgg cgatcggcga atttttcttc
     6361 cacaagagaa aggcgccctt tttcctgcgc ttccttttct attttctcat tatgattcaa
     6421 cagtatctca tgttgttaat cgctgccgcc ggtcttttcg atctgtggct cgattttcgc
     6481 aaactctcca caccagcgcc aaaagttaac aaaaaccctt agcaataaat taggaggata
     6541 agatgaaagt catattgaaa gaaaatctgg aagacctggg aaagatcggc gatatcgtga
     6601 acgtttccga tggatttgcc agaaattact tgattccgaa gggtttcgcc gccgaagcaa
     6661 ccagtaaatc cattcagtcc ctcgaacatg agcgcaaacg gatactccaa aaagcggaaa
     6721 aggagaagca acaagctgcc gctctcgtgg aaaagcttgc cgggttcacc tgtgtcattg
     6781 ctaggcgggt gggggaacag gataagctct ttggttccgt gaacaacaag gacatcgaaa
     6841 aggccttgaa ggaaatgggc ctggaaatcg accgtaaaac catcgtcatg tccgaaccga
     6901 tcaagaccct gggggaattc ccggtgaaaa tcaaacttcc cgccggtttg gcggcggaaa
     6961 tcaacattca ggtcgtaaag gaaggataaa ggataaaacc ttgccggacg ccagagagac
     7021 agatttctcg cttcatcgca tccctcccca gaacatcgag gcggaacaat ccgttctggg
     7081 agggatactc cttgacaacc agggattgaa cg
//
LOCUS       DAOSMF010000092         7080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_11749_length_7080_cov_21.814377, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000092 DAOSMF010000000
VERSION     DAOSMF010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 7080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7080
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11749_length_7080_cov_21.814377"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="PLT63_08500"
     CDS             complement(<1..845)
                     /locus_tag="PLT63_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98802.1"
                     /translation="MSREIKVQDLNPQSFIDEKVKEIRRIVGKGLAINALSGGVDSSV
                     VTALGHRALGDRLRSYYIDTGLMRQGEGKQVVDLFAALGIKVQIVPAGREFFRALKGI
                     TDPELKREAITQTFYRQVFKKLIMDSGAKCLFHGTILTDIDETVAGIKRQHNIFAQIG
                     IDPQKEFGYKIIEPLVELRKDGVRKVGQALGLPEALFNRIPFPGPALAARVLGEVTPE
                     RVRIVRKATTIVEKILAGTGSFQYLAVLHEDRVTGMRDGKRVFGMQIEVRCWESLDAR
                     IAAPT"
     gene            complement(878..2059)
                     /locus_tag="PLT63_08505"
     CDS             complement(878..2059)
                     /locus_tag="PLT63_08505"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOD98803.1"
                     /translation="MAMAKKIEGFITQSSWIRRMFEEGIRLKQEFGAGNVYDFSLGNP
                     NVEPPEEFRQALREVAAADIPGKHAYMPNAGYPETRRAVADYLFREQGVAITAEQVVM
                     TVGAGGALNVVFKTILDPGDEVLIPTPFFVEYRFYVDNGGGITVTVPTREDFSLDLTA
                     IEAAINKNTKAVLINSPNNPTGRVYDVASLEGLAAVMRRKSGDYGRTIYLVSDEPYRD
                     IVYDGVQVPGVMKLYGHSILVFSYSKSLSLSGERIGYIAVHPECEDAADLMGGLVLCN
                     RMLGFVNAPALMQRVIARLQGVNVDAGIYKRKRDLLCEGLASVGYEVSKPEGAFYLFQ
                     KTPIADDVAFVRALQEERILAVPGSGFGGPGFIRIAYCVDDATIKNAMAGFGRVLNKF
                     Q"
     gene            2279..2740
                     /locus_tag="PLT63_08510"
     CDS             2279..2740
                     /locus_tag="PLT63_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98804.1"
                     /translation="MDIIILTALGIAATAFIILRRWVESRRYGKFQPSDEITAAFESF
                     RVEKNKRYYLSGPDNYPHAIIGIEKEWRLETDLWKQRDLSGQTMKELVQNMHSRALER
                     NLTLYGFSILDDRDRNIGVWYSIMGLDMTVEIRGEYSVVIHTPPIDTYGVN"
     assembly_gap    2743..2842
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2843..4018
                     /locus_tag="PLT63_08515"
     CDS             <2843..4018
                     /locus_tag="PLT63_08515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOD98805.1"
                     /translation="GKVNIMKRSLAAIAIFFLGVFPVLISACANSSQDASGAKDGRER
                     AVPVTAATVIRKEMPIEIKTFGNVQAKSSVQIKAEVDGALAKVHFRKGQNIRRGDLLF
                     SIDPRSYQAALAQAQANLLRDRALLEHALLTAKRARELIQRGYIARSDFDKAQSEADA
                     LSATIKADEAAIAHAKLQVERCSIRSPINGKAGDLLVSEGALIKAKDMTMVVINQLRP
                     IEVFFSIPQADLPAVRSEMSQRKLKVKAYLPKDNRPPEEGELFFIDNAIDKSTGSVQL
                     AGTFDNERDLLWPGQYVLVTLVLAVQKDALVVPSIAVQNGQEGRFVFVVKPDRTVEVR
                     PVTTRMAAGGETVIEKGLQAGEQVVTDGHLGLVPGTRIEIKNPASDKADGAIPQGRS"
     gene            4015..>7080
                     /locus_tag="PLT63_08520"
     CDS             4015..>7080
                     /locus_tag="PLT63_08520"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOD98806.1"
                     /translation="MNLSGLFIRRPIMTMLCMLSIVFAGLIGYRLLPVSDLPTVDFPT
                     ISVSASLPGASPETMASTVATPLEKEFATIAGIDSMSSVSNQGSTRITLQFVLSRDID
                     AAAQDVQSAISRAIRRLPENLPTPPSFRKVNPADQPILYLTLTSPTLPMSVLDEYGQT
                     MIAQRISMVAGVAQVQVYGSQKYAVRIELDPNELASRGIGIDEVSTAVSNYNVNLPLG
                     TLSGPQSLSTIQSDNQLKEAKQYLPLIVAYRNGQPVRLQELGVVRDSIENDRVAAWHV
                     TPQSISRSIILAIQRQPGTNTVQVANAVKALLPSFREKLPASVSLGVLRDSSIPIKDS
                     AKDVQFTLYLTLALVVMVIFLFLRNLSATVIPSLTLPVSLIGTFAVMYLFDYSIDNLS
                     LMALTLSVGFVVDDAIVMLENIVRHMEMGKSRMQAAFDGAREVGFTIVSMTLSLSAVF
                     IPVLFMGGIVGRLFREFSVTIGVAVLVSGFVSLTLTPMLSSRFIRTPGKEQHGRAYQA
                     IEAGFESMLRFYSRTLLTILAHGRETMIFSGVVLVATTVLFYVIPKGFIPSEDRAQIS
                     VQTEAAQNISFNAMVQHQLALVDIVRRDPNVARFMCSVSSGGSSGSNTGNMFIVLKPR
                     RERDLTADQVISELRPKLASVPGIRAYLNNPPPINLGGRISRSLYQVTFQGTDTKELF
                     RYAAKMEQQMRGMEELQDVSSDLQLKNPELHVEIDRDQAFIMNVTPRQIEEALYSAYG
                     DRQISTIYTPNNDYQVIMELRPEYQSDPSVLSMLYIRSSQGQLVPLRAITKLREDVGP
                     LSINHSGQLPSVTVSFNVKPGFSLGQAVEKVRGIAGASLPAGMTYAFQGTAQAFQASL
                     ESMGILLMLAIVVIYIVLGILYESLYHPVTILSALPFAGFGALLTLMAFRSELSIYAF
                     VGIVMLVGLVKKNGIMMVDFAIEAQRQKGKSAHDAIHEACLIRFRPIMMTTMAALVAG
                     LPIALGYGAGAESRRPLGLAVVGGLLFSQTLTLYVTPVFYIYMEKFRTRI"
BASE COUNT         1452 a         2146 c         1930 g         1452 t
ORIGIN      
        1 tgggtaggcg cggcgatccg ggcgtcaagg ctctcccagc agcgcacctc gatctgcatc
       61 ccgaagaccc ttttaccatc ccgcatcccc gtaacccggt cttcatggag gacggccagg
      121 tactggaaac tcccggtccc cgccagaatt ttctccacga tggtcgtggc cttccggacg
      181 atgcggaccc gctccggcgt aacctcgccc aggacccggg ccgccagggc cggtccggga
      241 aaggggatac ggttgaagag ggcctccggc agacccaggg cctgcccaac cttccggacg
      301 ccgtcctttc gcagctccac caggggttcg atgatcttgt agccgaactc cttctggggg
      361 tcgatgccga tctgggcgaa gatgttgtgc tggcgcttga tgccggccac cgtttcatcg
      421 atgtccgtga ggatcgtgcc atgaaagaga catttggcgc cgctgtccat aatcagcttc
      481 ttgaatacct gccggtaaaa cgtctgggta atggcctccc gcttcagctc cggatcggtg
      541 atccccttca gggcgcgaaa gaattccctg cccgccggaa cgatctgcac cttgatcccc
      601 agggcggcaa atagatcgac aacctgcttg ccctcgccct gacgcatgag gccggtatcg
      661 atgtaatagg agcgcaggcg gtcccccagg gcccgatggc ccaaggccgt aaccacggag
      721 gaatcgacac cgccggacaa cgcgttgatg gcgaggcctt tgccgacgat ccggcggatc
      781 tcctttacct tctcatcaat gaaactttgc ggatttaaat cctgaacctt gatttccctg
      841 ctcatctttt ctcaccctgt ttacccttca ttgaaattca ttgaaattta ttcagcaccc
      901 tgccgaaccc ggccatggca ttctttatcg tggcatcatc gacgcaatag gcaatccgga
      961 tgaatcccgg accgccaaaa ccgctgcccg gcaccgccag aatccgctcc tcctgaaggg
     1021 cccggacaaa ggctacgtcg tcggcaatgg gtgttttctg gaagagataa aaggcgccct
     1081 ccggcttgct gacctcatag cccaccgagg cgaggccttc acagaggaga tcgcgcttgc
     1141 gtttatatat ccctgcatcc acattgactc cctggagacg ggcgatgacc cgctgcatca
     1201 gggccggggc gttgacgaag cccagcatgc ggttgcacag gaccagaccg cccatcaggt
     1261 cggcggcgtc ctcacattcc ggatggacgg cgatatagcc gatccgctcc ccggacaggg
     1321 agaggctctt ggaataggaa aagaccagga tgctgtggcc gtaaagtttc atgacgccgg
     1381 ggacctgaac gccgtcgtac acgatgtctc gatagggttc atcggagacg agataaatag
     1441 tccggccgta atccccggat tttcgtcgca tgacggcggc cagaccctcc agcgaggcca
     1501 cgtcataaac ccggccggta gggttgttcg gagaattgat cagcactgcc ttggtatttt
     1561 tattgatggc cgcctcgatg gcggtcagat ccagggaaaa gtcttccctg gtggggacag
     1621 tgacggtaat gccgccgcca ttgtccacat agaaacggta ttcgacgaaa aagggggtgg
     1681 gaatgaggac ctcgtcgccg ggatcgagga tggttttaaa gacgacattg agcgcccccc
     1741 cggctccgac ggtcatgacg acttgctccg ccgttatggc cacgccctgc tcccggaaaa
     1801 ggtaatccgc cacggcccgc cgcgtctcgg gataaccggc attgggcata taggcatgct
     1861 tccccggaat atccgcggcg gccacttccc ggagcgcctg gcggaactcc tccggtggct
     1921 ccacgttggg gttgcccaga ctgaaatcat agacattccc agccccgaat tcctgcttga
     1981 gccgtatccc ctcttcgaac atccttctta tccacgacga ttgcgtgata aacccctcga
     2041 tcttttttgc catggccata gccggtctcc tttaaacaac gtgtattttt gtccttccgg
     2101 aaatgaggca gtaccgttct tttcccgagc ggagcggagt ataagaagag cgccatggga
     2161 tgtcaatgaa aaagcatgaa ccgatcggtg attgaatgat gcattacgat ataatttctt
     2221 tacatcttgc ttttttgtat ttagcatgat gtgcatttta tccccggaag agaaggctat
     2281 ggacataata attttgacgg ccctgggaat agcggcgacg gcgttcatca tcctcaggag
     2341 atgggtggaa agcaggcgct acggtaaatt ccagccgagc gacgagatta ctgcggcatt
     2401 cgagtcattc cgcgtggaga aaaacaaaag atattacctc agcggccccg ataattatcc
     2461 ccatgccatt atcggcatcg agaaggagtg gagacttgag acggatttat ggaagcaaag
     2521 ggacctctcc gggcaaacca tgaaggaact ggtgcagaat atgcactcca gagccctgga
     2581 gcggaatctg accctatacg gattcagcat ccttgacgat cgagatcgaa acatcggcgt
     2641 ctggtattcc atcatggggc tggatatgac cgtcgagatc cggggggaat acagcgtcgt
     2701 catacacacg cctcccatcg acacctacgg cgtcaattga tgnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnggaaaggt caacatcatg aaaagatcat tagccgccat
     2881 cgccatcttt ttcctaggcg tatttcccgt cctgatctca gcgtgcgcca attcatccca
     2941 ggatgcaagc ggggcaaaag atggccggga gcgggccgta cccgtaacgg cggcgacagt
     3001 gatcaggaaa gaaatgccca tcgaaatcaa aacgttcgga aatgtccaag ccaaatccag
     3061 cgtccagatc aaagcagagg tggatggcgc cctggccaag gtgcatttcc gcaaaggcca
     3121 gaatatccgg cggggagatc tcctcttctc catcgaccca cgctcctatc aggcggcgct
     3181 ggcccaggcg caggccaacc tcctgcggga ccgggcgctg ctggaacatg ccctgctcac
     3241 tgctaagcgg gccagggaac tcattcagag gggctatatt gcccgcagcg acttcgacaa
     3301 ggcccagtcg gaagccgacg cgctgtcggc gacgatcaaa gccgatgagg ccgccatcgc
     3361 ccatgccaaa ttacaggtgg agcgatgcag catccgcagc ccgatcaacg ggaaggccgg
     3421 tgatctgctc gtatccgagg gggcgctgat caaggctaag gatatgacca tggtggtcat
     3481 caaccagctg cggccgatcg aggttttttt cagcatcccc caggcggacc ttccggccgt
     3541 ccgcagcgag atgtcccaac ggaaactgaa ggtcaaggcc tacctgccga aagataaccg
     3601 ccccccagag gaaggggagt tgttcttcat agacaacgcc atcgataaaa gcaccggctc
     3661 cgtgcaactg gccggtacat tcgacaatga gcgggatttg ctctggcccg gccagtatgt
     3721 actggtaacc ctcgtgctgg cggtccagaa agacgccctg gtggtaccat ccatagccgt
     3781 ccagaacggt caggagggta gattcgtctt tgtcgtcaaa ccggaccgca ccgtcgaggt
     3841 ccgaccggtt acgacccgca tggccgcggg aggggagacg gtgatcgaga agggattgca
     3901 ggccggggag caggtggtca ccgacggcca cctcgggctt gtccccggca ccaggataga
     3961 gatcaaaaac cccgcgtccg ataaggcgga cggcgcgatt ccacagggac gttcatgaac
     4021 ctctccgggc tgtttatccg ccgtcccatc atgacgatgc tctgcatgct gagtatcgtc
     4081 ttcgccggtc tgatcggcta tcgtctcctg cctgtgagcg acctgccgac ggtcgatttc
     4141 cccacaatca gtgtcagcgc ctcccttccc ggcgccagcc cggaaaccat ggcttccacc
     4201 gtggccacgc ctctggaaaa ggagtttgcg accatcgccg gcatcgactc catgagttcc
     4261 gtaagcaacc agggctccac ccggatcacc ctgcagttcg tcctgagccg ggacatcgac
     4321 gccgccgccc aggacgtgca gtccgccatc tcccgcgcca tccgccggct gccggaaaat
     4381 ctgcccaccc ccccgtcttt ccgcaaggtc aatcccgccg atcagccgat tctctatctg
     4441 acgttgacga gcccgaccct gcccatgtcc gtcttggacg agtacggtca aaccatgatc
     4501 gcccagcgga tttccatggt ggcgggggtt gcgcaggtac aggtttacgg gtctcagaag
     4561 tacgccgtcc gcatcgaact ggaccccaac gagttggcct cccggggtat cggtatcgat
     4621 gaggtatcca ccgcggtgag caattacaac gtgaatctcc ccctcggaac attgagcgga
     4681 cctcaaagtc tatccacgat tcaatcggac aaccagctca aggaggcaaa acaatacctg
     4741 cccctcatcg tggcgtaccg caacggccag ccggtgcggc ttcaggaact aggggtcgtc
     4801 cgggacagta tcgaaaacga cagggtggcg gcgtggcatg tcacaccaca gtccatcagc
     4861 cgctccatca tcctggccat ccagcggcaa ccgggaacga ataccgtcca ggtcgccaat
     4921 gccgtcaagg ccctgctgcc ctcatttcgc gagaagctgc cggcttctgt ttccctgggc
     4981 gtactccggg acagcagcat ccccatcaag gattcggcaa aggatgtgca gtttaccctc
     5041 tacctgaccc tggccctcgt ggtaatggtt atctttctat tcctgcgcaa cctctcggcg
     5101 accgtcatcc cgagcctgac tctccccgtg tccttgatcg gcacttttgc cgttatgtat
     5161 ctctttgatt acagcatcga caacctgtcg ctcatggccc tgactttgtc ggtgggtttc
     5221 gtcgttgatg acgccatcgt catgctggaa aacatcgtca ggcacatgga gatggggaaa
     5281 agccgcatgc aggccgcttt cgacggcgcc cgggaagtgg gtttcaccat cgtgtccatg
     5341 actctttccc tgtccgccgt ttttatcccc gtgctgttca tgggcggcat cgtcgggcgg
     5401 ctcttccggg agttttccgt cactatcggc gtggcggtcc tggtcagcgg cttcgtctcc
     5461 ctgaccttga cccccatgct gtccagccgg tttatccgta cccctgggaa agaacagcat
     5521 ggccgggcgt atcaggccat cgaggcgggg ttcgaatcca tgctcaggtt ttacagccgt
     5581 acccttctta cgattctggc tcacggccgt gaaaccatga tcttttccgg agtggtcctc
     5641 gtggccacca ccgttttgtt ttacgtcatc ccgaagggtt tcatcccctc cgaagaccgg
     5701 gctcagatct cggtgcagac ggaggcggcc caaaacatct ctttcaacgc catggttcag
     5761 caccagctcg ccctggtgga tatcgtccgg cgtgatccca atgtggcccg gtttatgtgc
     5821 agcgtctcat ccggaggcag cagcggctcc aacacaggca acatgttcat cgtcctcaag
     5881 ccgcgccggg aaagggatct caccgcggat caggtgattt cggaactacg tcccaagctg
     5941 gcctccgttc ccggtatccg ggcctatctg aacaaccctc cgcccatcaa tctggggggc
     6001 cgcatctccc ggagcctcta ccaggttacc tttcaaggca cggatacgaa agagctgttc
     6061 agatatgccg cgaaaatgga gcaacagatg cgtggcatgg aggaactgca ggacgtcagc
     6121 agcgacctgc agttgaagaa tccggaactg cacgtcgaaa tcgaccggga tcaggcgttc
     6181 atcatgaacg tcaccccgag gcagatcgaa gaggccctct acagcgccta tggagatcga
     6241 cagatttcaa ccatttatac accgaacaac gattaccagg taatcatgga actgcggccc
     6301 gagtaccaga gcgacccctc cgtgctgtcc atgctctata tccgctcttc ccaggggcaa
     6361 ctggtgcccc tccgggcgat cacgaagctc agggaggacg ttggtcccct gtctatcaac
     6421 cattccggac agttgccctc cgtgaccgtc tccttcaacg tcaaaccggg cttctcgctc
     6481 ggtcaggcgg tggaaaaggt ccggggcatc gccggcgcct ccttgcccgc cgggatgacg
     6541 tacgcattcc agggcacggc tcaggccttc caagcttctc tcgaaagcat gggtatcttg
     6601 cttatgctgg ccatcgtggt catctatatc gtcctgggca tcctgtacga aagcctttat
     6661 catcccgtta ccatcctctc cgccctgcct ttcgccggct tcggggccct gctgacgctc
     6721 atggccttcc gttccgaact gtccatttat gccttcgtgg gcatcgtcat gctggtggga
     6781 ctcgtaaaga agaatgggat catgatggtg gattttgcca tcgaggccca gcggcaaaag
     6841 gggaaaagcg ctcatgacgc aatccacgag gcgtgcctga ttcgtttccg gccgatcatg
     6901 atgaccacta tggccgccct cgtggccggt ctgcccatcg ccctcgggta tggcgccggc
     6961 gcggaatccc gccgtcccct gggactggcc gtggtcggcg gtctcctctt ctcacagacg
     7021 ctgaccctct atgtcacccc tgttttctac atctacatgg agaaattccg gacacgcatc
//
LOCUS       DAOSMF010000093         7073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_11769_length_7073_cov_16.363351, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000093 DAOSMF010000000
VERSION     DAOSMF010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 7073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7073
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11769_length_7073_cov_16.363351"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /gene="prxU"
                     /locus_tag="PLT63_08525"
     CDS             complement(<1..691)
                     /gene="prxU"
                     /locus_tag="PLT63_08525"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_084511687.1"
                     /note="Most members of this family contain a
                     selenocysteine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(440..442),aa:Sec)
                     /transl_table=11
                     /product="thioredoxin-dependent peroxiredoxin"
                     /protein_id="HOD98807.1"
                     /translation="MTEEIKAGCARPTGGPVGEEPAAKQETPQHTVKEVKSMVTVGKK
                     APDFTAPAYHNGKFINVQLSEYLGKWVILCFYPGDFTFVUATEISAVAEKYHEFKELG
                     VEVLSMSVDSIFVHKMWNDHELSKMVKGGVPFPMLSDGGGKVGSIFSVYLEDAGVEAR
                     GRFIIDPDGVIQGFEVLTPPVGRNIMESIRQIQAFQHVRDSKGTEATPSGWRPGKLTL
                     KPGPDLVGRVWE"
     gene            complement(958..3153)
                     /gene="katG"
                     /locus_tag="PLT63_08530"
     CDS             complement(958..3153)
                     /gene="katG"
                     /locus_tag="PLT63_08530"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF011635.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HOD98808.1"
                     /translation="MSEDSKCPVTGRMGKPISGGGTSNRDWWPNQLNLKILHQHSRLS
                     NPMEEAFNYAEEFKKLDLEAVKNDLYTLMTDSQDWWPADWGHYGPLFIRMAWHSAGTY
                     RMGDGRGGAGSGSQRLAPLNSWPDNVNLDKARRLIWPIKQKYGKKISWADLMILAGNC
                     AIESMGLKTFGFAGGREDVWEPEEDIYWGSEDTWLGDKRYTGDRNLENPLAAVQMGLI
                     YVNPEGPNGEPIPSASAHDVRQTFARMAMNDEETVALVAGGHTFGKAHGAGPASHVGP
                     EPEGAPIEDQGLGWKSNFGSGKGGDTISSGIEGAWKPNPTKWDMGYLKVLFKYEWELL
                     KSPAGAYIWLAKDVEDEDMAVDAHDPSKKRRPMMTTADLSLKFDPAYEKIARRYLANP
                     DEFADAFARAWFKLTHRDMGPRSRYLGPEVPAEELIWQDPIPAVNHKLIDAKDIAVLK
                     GKISASGLSVSELVSTAWASASTFRGSDKRGGANGARIRLAPQKDWEVNQPARLAKVL
                     KTLEGIQSDFNGAEKDGKKVSMADLIVLGGCAGLEQAARNAGHEVSVPFTPGRMDASQ
                     EQTDAASFAVLEPKADGFRNYQRAKYAVSAEEMLVDRAQLLTLTAPEMTVLIGGMRVL
                     NTNFGGARHGVFTKRPEALTNDFFVNLLDMGTEWKATSEDKDLFEGRDRKTGELRWTA
                     TRADLIFGSNSQLRALAEVYGCEDSQGKFLYDFVAVWNKVMNLDRFDLA"
     gene            complement(3273..3740)
                     /locus_tag="PLT63_08535"
     CDS             complement(3273..3740)
                     /locus_tag="PLT63_08535"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HOD98809.1"
                     /translation="MLTKESIVQKLKERGLRITPQRLSIIDVLVKKGHLHPSASFIYN
                     EAKKKGIPLSLSSVYENIKELCRYKIIKSLEFDGRENRCEANLGEHINLICEHCGKII
                     DYELPVSINQTDVAKRTGFMITHNRLEYYGYCAECTKGKGWPISDHKPLGAPL"
     gene            complement(4335..4868)
                     /locus_tag="PLT63_08540"
     CDS             complement(4335..4868)
                     /locus_tag="PLT63_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98810.1"
                     /translation="MKIFNRHDRVIPVDKDTLGSVLNTLSGPEDALWPRESWPPMVLD
                     QGLRVGAAGGHSLVKYRVAEYVPGHRVVFQFDPQGLMDGVDGRHFFEVVPRRTDVIIR
                     HDLEGNCNLGMWLRWNFLVRPLHDTLIEDAFDKVESRFRGTVVKRSHWGPWVRFLRWM
                     VRRRRAEAAAREQGTSK"
     gene            complement(4852..5445)
                     /locus_tag="PLT63_08545"
     CDS             complement(4852..5445)
                     /locus_tag="PLT63_08545"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOD98811.1"
                     /translation="MGRNAHFQNEDFIKAVLTIAAEHGPAAVTITAVAEAVGAPVGSV
                     YHRFPSRDVLMAEVWLRVVASFQEEYLAFLEKGDGLGAALHTPRWVRRYPVEARILLM
                     YRREELVAGDGPESLKFEVQEIARRLSDGVVAFAARTFGRTGREEVGRIVFATIDAPY
                     AAVLRYIRLGKKPPRAVEDYLTKTYDAIIGGSYEDIQ"
     gene            complement(5901..6161)
                     /locus_tag="PLT63_08550"
     CDS             complement(5901..6161)
                     /locus_tag="PLT63_08550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOD98812.1"
                     /translation="MAYNIVYKKSVYRDLKKLSKTEAKRILDLIETELIKMPESNPTL
                     KGQFAGLRKYRIGDYRVIYALLGLDIIILRIGNRKEVYKGEI"
     gene            complement(6151..6369)
                     /locus_tag="PLT63_08555"
     CDS             complement(6151..6369)
                     /locus_tag="PLT63_08555"
                     /inference="COORDINATES: protein motif:HMM:NF039402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="HOD98813.1"
                     /translation="MSTAISIRLPKSLASQLDNISKETERPRSFIIQKALESYIEDYA
                     DLQIALDRLYDKGDAVISSKEMRKSLGL"
BASE COUNT         1605 a         1920 c         1708 g         1840 t
ORIGIN      
        1 cctcccagac tctgccgaca aggtccggtc ctggtttcag ggtcaatttg ccgggacgcc
       61 agccggaagg cgtagcttcc gtgcctttgc tgtcacggac atgctggaaa gcctgaatct
      121 gccggatgct ttccataata tttcgtccca ccggtggcgt aagaacttca aatccctgaa
      181 ttaccccgtc aggatcgatg ataaaccgtc cacgggcttc tacgccggca tcttcgagat
      241 atacgctgaa gatgctgccc accttgcctc caccgtcgga aagcatggga aagggtacac
      301 cgccttttac cattttggag agctcatggt cattccacat tttgtggacg aagatgctgt
      361 ccacactcat ggaaagcacc tcaacgccca actctttgaa ttcgtgatat ttctccgcga
      421 ccgcggagat ttcggtcgct cagacaaaag taaaatctcc tggatagaaa cacaggatca
      481 cccatttgcc gagatactcg gacagctgaa cgttgataaa tttaccatta tgatacgcgg
      541 gtgcagtaaa atcgggagcc ttcttgccga cggttaccat gcttttcacc tccttgacag
      601 tgtgttgcgg ggtttcttgt tttgccgcgg gttcttctcc gaccgggccg ccggttggtc
      661 tggcgcaacc ggccttgatt tcttccgtca tatgtttttg cctcctttcc acagcttcct
      721 tttaaaaata ctcattgtaa ctttgcataa agatattatc gacataaata aggaataata
      781 ttttatccat tcctcgagtt tatcaaaatc ttcccatgtt ctgccaagga gcggatcaag
      841 ggcagatgtg tgaaccattc ttatcaagcg gcggtgttaa gccgtccgct ggaatcggtt
      901 attttaactg gcttcgtgct tctccctgtc agagaagcac gaagacgttt tttatgatca
      961 agcaagatcg aaacgatcaa ggttcatgac cttgttccac accgctacaa aatcgtacag
     1021 gaactttccc tgagagtctt cgcatccgta aacctccgcc aatgcccgga gttgtgagtt
     1081 agaaccgaag atcaagtccg ctcgggtggc tgtccacctg agctcgccgg ttttgcgatc
     1141 acgaccctcg aacagatctt tgtcttcgga tgttgccttc cattccgttc ccatgtcgag
     1201 cagattaacg aagaagtcat ttgtcagcgc ctccggccgt ttggtgaaaa caccgtgccg
     1261 agcgcctccg aagttggtgt tgagtacgcg catgccaccg ataagtacag tcatttccgg
     1321 cgcggtcagc gtcagaagct gtgccctgtc aacgagcatc tcctctgccg atacggcata
     1381 tttggccctc tggtagttgc ggaagccgtc tgccttcggt tccaggacgg caaaggaggc
     1441 cgcatcggtc tgctcctgcg aggcgtccat ccgccccggt gtaaaaggaa ccgacacctc
     1501 atgccccgca ttcctggcag cctgttcgag gcccgcacag ccgcccagaa cgattaggtc
     1561 ggccatcgat accttcttac cgtctttctc cgcgccgttg aaatcgctct ggatcccctc
     1621 aagggtttta agcacctttg ccaaccgggc aggctggttg acctcccaat ccttctgcgg
     1681 ggcaagccga atgcgggcgc cgttcgcacc tcctctcttg tcggagccgc ggaaagtgga
     1741 tgccgaagcc caggcagtag agacaagctc cgacacggag aggccggaag ccgatatctt
     1801 gcctttgagg acggcaatgt ccttggcatc aatcagttta tgattgacag cagggatggg
     1861 atcctgccag ataagctctt cggccgggac ctccgggccc aaatagcgcg agcggggtcc
     1921 catgtcgcgg tgggtcagct tgaaccacgc ccgggcaaaa gcgtcggcaa attcatcagg
     1981 attagccaga taacgccgcg cgattttctc gtaagcagga tcaaacttga gggaaagatc
     2041 tgccgtggtc atcatgggcc gacgtttttt agatggatca tgggcatcaa cagccatatc
     2101 ctcgtcttct acatcttttg ccagccatat ataggcccct gccgggcttt tgagaagttc
     2161 ccattcatat ttgaataata ccttcagata gcccatgtcc catttggtcg ggtttggctt
     2221 ccaggcgccc tcgatgccac tgctaatcgt gtctccaccc ttgccgctgc cgaagttgct
     2281 tttccatccg agcccctgat cttcgatagg agccccctct ggttcaggcc ccacatggga
     2341 cgccgggccg gcgccgtgag ctttaccaaa ggtgtgcccg ccggcgacca gcgcgacggt
     2401 ctcttcgtcg ttcatggcca tacgggcgaa agtttgacga acatcatgag cagaagcgga
     2461 tgggataggt tcaccgttcg gcccttccgg gttcacataa atcagtccca tctgcacggc
     2521 ggcaagcgga ttttcgagat tccggtcacc tgtatatcgc ttgtcgccga gccaggtatc
     2581 ctcgctgccc caatatatgt cctcttccgg ctcccatacg tcctcacgcc ccccggcgaa
     2641 gccgaaggtc ttcaatccca tcgactcgat ggcacagttg ccggcaagga tcatgaggtc
     2701 ggcccaggat atctttttgc cgtatttttg tttgatgggc cagatcaggc ggcgtgcctt
     2761 gtctaggttc acattgtcgg gccagctatt gagaggtgcc aggcgctggg agcctgaccc
     2821 cgcgccacca cggccgtcgc ccatgcggta agtacctgcg ctgtgccacg ccatccggat
     2881 gaaaagcgga ccgtaatgac cccagtcggc cggccaccag tcctgcgagt cggtcatcag
     2941 cgtataaagg tcattcttca cggcctcaag gtcgagtttc ttgaattcct cggcgtagtt
     3001 gaacgcctcc tccatcggat tgctcaaccg ggagtgctga tggagaatct tcaagttcaa
     3061 ctggttcggc caccagtccc ggttcgaagt gccgccacct gaaattggtt tgcccatcct
     3121 gcccgttacc gggcacttgc tgtcttcgct cataatttgt cctcctttca tttttttgta
     3181 ttatgtctgt ttttatgctc caaccaatcc gattgtttta tctggaatca ttccagataa
     3241 aagagtaaaa aaatttgaat acggaaaccg atctacagag gcgctcccag cggcttgtga
     3301 tcggagatcg gccacccttt acctttggtg cattctgcac agtatccgta atactccagt
     3361 ctgttatggg tgatcatgaa gcccgttctt tttgccacat ctgtttgatt tatggaaacg
     3421 ggcagttcgt aatcaataat cttcccgcaa tgttcgcaaa taagattgat gtgctctcca
     3481 agattagcct cgcagcggtt ttccctgccg tcgaactcaa gtgatttgat gatcttgtac
     3541 cggcacagct cctttatgtt ttcatagacg gatgaaaggc ttagagggat gcccttcttt
     3601 ttcgcctcat tgtatatgaa gcttgcactt ggatgaagat gccccttctt gaccagcaca
     3661 tcaataatag aaagtctttg aggggtgatt cttaatcctc tttctttaag cttttgaaca
     3721 attgattctt ttgtcaacat gttttactcg gaaccattcc agattgaggt tcaaattatc
     3781 cttcattcaa tcggctgtca agatattttt tgctaatata taatcacatg agaaaaataa
     3841 tgattcgaag attggtatct caaagaagtt tcattttgct gcgtatatgc agaaaggaaa
     3901 ttttaccacc tcccaatgaa aaattcatgt ttgaaaacgt gcacattacg ccccgcattc
     3961 aatgccttga ccaatgtatc acgagtaacg ctttgcagat ggagacgata tttttcaccc
     4021 gctatatttt ccctggtctt gtctaaatag tattggttaa aagccgctac tactcaagcc
     4081 cttttccatc atttgttgag taaatgaagg atacgagagg ccatttgaat cagaatcaac
     4141 ctattatcaa tgtgaacctt tcgacccatt ataatagatt cggacttacc ggccaacatc
     4201 tccctttgta catctaagag ataaaatacg catccgtcga atgaccttta tttgggtggg
     4261 gaggttgccg cattttccta cggcagggaa cggatgccgg ccagaggaag gtcgtgggat
     4321 gatgcggtct gctgctattt ggacgtcccc tgctccctgg cagccgcttc cgcgcggcgc
     4381 cgccgcacca tccagcgcag aaaacggacc cacggccccc agtgcgagcg ctttaccacc
     4441 gtgccccgga acctgctttc caccttgtca aaggcatcct cgatcagcgt gtcgtggagc
     4501 ggccggacca gaaagttcca tctcagccac atgccgaggt tgcagttgcc ctccagatcg
     4561 tgacggatga taacgtccgt gcgtctaggt acaacctcga agaaatgccg gccgtccaca
     4621 ccgtccataa gaccctgcgg gtcgaactgg aataccaccc ggtgccccgg aacatactcc
     4681 gctacccggt actttactaa gctgtgacca cctgccgctc cgacacgcag accctggtcc
     4741 aggaccatcg gcggccagct ctcccgcggc cacagggcat cttccgggcc ggaaagggtg
     4801 ttgagaacgg aacccagggt atccttgtcc acagggatta cccggtcatg tctattgaat
     4861 atcttcataa ctacctccta taatggcatc atatgttttt gtcagatagt cttcgaccgc
     4921 ccgtggcggc ttctttccca accggatgta gcgcaggacc gccgcatagg gcgcatctat
     4981 ggtcgcaaaa acgatccgtc ccacctcctc gcgccccgtc cggccgaagg tcctggccgc
     5041 aaaggcgacg actccgtcac tgagcctgcg agcgatctcc tgcacctcga acttcaggct
     5101 ttccggcccg tcgcccgcca ccagttcctc ccgccggtac atcaggagga tgcgggcctc
     5161 tacaggatac cggcgcaccc accttggtgt gtgaagcgct gctcccagcc cgtctccttt
     5221 ttccaggaag gccagatatt cttcctggaa agaagcgacg acccgcagcc atacctccgc
     5281 catgaggacg tcgcgggacg ggaaccggtg atacaccgat cccaccggcg cgcccaccgc
     5341 ttcggccaca gcggtaatgg ttacggcggc aggcccgtgt tccgcggcga tcgtcaggac
     5401 cgccttgata aaatcctcgt tctggaaatg cgcatttctt cccatttggg cgttatatta
     5461 gaatagacat tctaatatgt caagattttt ttcagtccat aaagaatagg attgaccgtt
     5521 aacgccaggt tgtaacaatt aaagaagacg gcctatattg attgcttatg catagcggcc
     5581 agttgtaccg gcagtgctga ggccattctg aatgacggtg tttttttcgg aaaagtgcgg
     5641 acctaattag gtgtaaaagt taatctctcc ccgttcagtt agcgaatttt ttcacgggaa
     5701 gaaatttgta acttctcatc aacattcaat acatctgcaa ggttgtcaag gatgatgcct
     5761 tgcagatgaa gggtacttta gataagtaca actacccact gcaatacccg gctatggccc
     5821 gaatctctat gtaccgcccg atgcggacct atgcatgccg ggtcatgtgg ggctgggtga
     5881 aaaaaccccc ggttacgcga tcagatttcg cctttataaa cttccttccg attgccaatc
     5941 ctgaggatga tgatgtcaag gcccaacaag gcatagatca ccctgtagtc accgatgcgg
     6001 tacttgcgaa gtccggcaaa ctgccccttg agcgtgggat tggattcagg cattttgatc
     6061 aattccgtct caatgaggtc aaggattcgt tttgcttctg tcttcgatag tttcttgagg
     6121 tcgcggtaga ccgatttctt gtaaacgatg ttataagcca agggacttcc tcatctcttt
     6181 ggatgaaata actgcatcac ccttgtcgta gagtcggtcc aaggcgatct ggagatccgc
     6241 gtaatcctct atataggact ctagtgcctt ttgaatgatg aaagagcgag gtctttcggt
     6301 ctctttagat atattgtcga gttggctggc aaggcttttt ggaagccgaa ttgatattgc
     6361 tgtgctcata gcgtacctcc tgtcattgat gtcttacaat gtatgcaata gtacctgttt
     6421 tgtcaagtcc attctcaaaa tcttaccagt atttgtattg accagccctt tatagttagt
     6481 tgataatgtg cacaggaagg taatatgtag caaatatggc aacaagccat ggtaatcagc
     6541 ttccgaaatt ggtgaagtgt tgttctcagg aaattctgaa gctgtggacc aaaacaattt
     6601 ataagagtca aaatgtgctg ctttcctcca ttattcaatg acttgaccaa gtaatccagg
     6661 ctcacgcttt gcagatgaag ggtactttag ataagtacaa ctacccactg caatacccgg
     6721 ctatggcccg aatctctatg taccgccaga tgcggacctg catgccgggt cgtgtggggg
     6781 ccgggagagc cccccggcta cccgattaga tttcgccttt ataaacatcc ttccgattgc
     6841 cgatcctgag gatgatgtta taggccaagg gacttcctca tctctttaga tgaaataact
     6901 tcatcaccct cgaaaatcta gcgaagattt ttgttgacat gggggaaaaa ctgtgagtaa
     6961 ttctcacggc acaaatttag gcaaggagtc tatcaaccga cacgatgggt aaccacaacg
     7021 cgctttccag cttttattac tggtattatt tttacggcgc gccggtctgc ctg
//
LOCUS       DAOSMF010000094         7050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_11806_length_7050_cov_25.675768, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000094 DAOSMF010000000
VERSION     DAOSMF010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 7050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7050
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11806_length_7050_cov_25.675768"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="PLT63_08560"
     CDS             complement(<1..422)
                     /locus_tag="PLT63_08560"
                     /inference="COORDINATES: protein motif:HMM:NF018805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid 1,2-hydratase"
                     /protein_id="HOD98814.1"
                     /translation="MDNYSPFCRALSLFLVLLITTAPAAGSADWRPALDPWNWHFPQD
                     HGNHPSFRTEWWYFTGNLTDGAGRRYGYQLTFFRQGIRRIAAAPANPWSIGDAYFAHF
                     AVTDVSRAKFFHADRISRSGPGLAGAATGRMDVWNLNW"
     gene            complement(482..2971)
                     /locus_tag="PLT63_08565"
     CDS             complement(482..2971)
                     /locus_tag="PLT63_08565"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOD98815.1"
                     /translation="MGTVTKSFLRYLVRRKSLTLLQLMGIAFGVAAALGMGLSALSAL
                     ASFTGAIEFLQGKSTHTLERHAGPMDEQILKEIMQDPAVHSFAPVIDRRLRYGDGAQI
                     RLLGIDPFLDAAIRPELSRRSITGAPHRDAREGMSFLLEARAVLMEANIAAAVGLAAG
                     GRLRTSQGEIEVIGTFPNPSGEPLLLMDIAHAQEIFLLPGRIDRIDLILTDEEGFLSR
                     WRQGFRIRSNRQNHDTLRAMLGAFRINLEALSLMALFVAVFLIYNTTMFAVVSRRRDA
                     GILRSLGASRGEVILAFMTEILILGLVGGALGSVMGYLLSRFLSGIIAGTVSNLYFFL
                     HPLPLPWSFQMLGVGILLGCGASILGSIYPLVELARTDPVKSLSGRAMNRRGGPAARK
                     AAAIGLGITLLSASLFLLPDGSIYIAFGALFGMVFGLSLMTGYAVVACTPPIRAALTC
                     LGGITGKIAAGNIRRNLGRTAIAVAAFMVALSLSIGMALMIGSFRESLVWWMSGQLRG
                     DLYISTATDKEVPEGFYHELLEMRDIAGVDAYRKVQITYRNKPMFVAAVNAPVLQRYT
                     RFAWLAGGDEHWEAVKRGAVIVSESFARNFAIGLGDSITLEGRNGPAELRVEAVFYDY
                     STEHGLVMMDRHTYLKLFADPTINSVAVFLEPDHPRRRQTLDLVRQRAGAYDLPVFTR
                     EEFFGNILAIFDNTFAITRSMRIMAIIIAFFGIAGALMTLFIERQSELGILRALGFST
                     GQVSLMTLLEAVGMGLMSFLLSAGVGTLFAVVLIRVINLRSFHWTIFFHPQLSPYLVV
                     AATAVLASLGAALYPIVMVLRTYPQMQIRAE"
     gene            complement(2990..3691)
                     /locus_tag="PLT63_08570"
     CDS             complement(2990..3691)
                     /locus_tag="PLT63_08570"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD98816.1"
                     /translation="MNVPVIRARDLWKIYDHNPALRGVGMEIARGEVVALIGKSGSGK
                     TTLLNLLAGLDRPSRGTIEVEGQNLESLGEKGRTLLRRNRLGFVFQFFNLLPTLTVLE
                     NVYLPLELAGISSPENAREALGKLGLEGKERRYPHELSGGEQQRVAIARAVVKKPALI
                     LADEPTGNLDTTTGRQILEMLTTRCRESGMSLLMVSHSPTATGFADRVLSMIDGVIAA
                     ADAGGAPMHAPAPRW"
     gene            complement(3704..4135)
                     /locus_tag="PLT63_08575"
     CDS             complement(3704..4135)
                     /locus_tag="PLT63_08575"
                     /inference="COORDINATES: protein motif:HMM:NF025892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HOD98817.1"
                     /translation="MDSTIREAIFRQVAQEPFARKFHLQLVELDEGYARVEMAFTPDM
                     ENIFGKAHGGALFALIDEAFETASNSHGTTAVALNMSIGFLASPSSGSILVAEAREIN
                     RTVRTAVYDIKVREKSDRLIASCQALVYRTGKPLPFLDKHR"
     gene            4707..>5271
                     /locus_tag="PLT63_08580"
     CDS             4707..>5271
                     /locus_tag="PLT63_08580"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOD98818.1"
                     /translation="MEFISVMEARRAVNFFDPEKPVSDDELRKIVETAALAPSGLNFQ
                     PWQVIVVRSPGAKEKLMLAASKQVKIIEAPVTLIVLADRDGWKAGHPTVEKVWDNLVR
                     LGYWNTGQRGGFEKGPEKLYGTPERSLAFAVKNTAFFSMALMLAAKDAGLDCHPMDGF
                     DHEAVKRAFDVPDNFFIPLLIAIGHFDA"
     assembly_gap    5272..5371
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5866..6177)
                     /locus_tag="PLT63_08585"
     CDS             complement(5866..6177)
                     /locus_tag="PLT63_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98819.1"
                     /translation="MTISEWLDSQEATGKDVAHIVLPDELSREEDPEETIFYNEIREC
                     SILCDGDHPFATVERYGNWYLSRGREKDNGPHTSQPQWWMFTKDRDLAVRTAQMHMEK
                     S"
     gene            complement(6335..6736)
                     /locus_tag="PLT63_08590"
     CDS             complement(6335..6736)
                     /locus_tag="PLT63_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HOD98820.1"
                     /translation="MEQRHQTHSVLIGYILWIFGFTGSHRFYYGKPVSGTIYFFTLGL
                     LGIGWLIDIFLIPSMARKAELRFTEGRIDYNIAWLLLTFLGLFGIHRFYMGKWITGIL
                     YLCSAGIFGIGYIYDYWTLNDQITALNSSNH"
BASE COUNT         1474 a         1924 c         2080 g         1472 t
ORIGIN      
        1 aaccagttga ggttccagac atccatccgg cccgtcgccg cccccgccag tcccggtccg
       61 gaacggctga tgcggtcggc atggaagaat ttggctcggg aaacgtccgt gacggcgaaa
      121 tgggcgaaat aggcgtcacc gatggaccag ggattcgcgg gggcggcggc gatcctcctg
      181 atcccctgcc ggaaaaatgt caactggtaa ccgtagcgac gtccggcgcc gtcggtgagg
      241 ttgccggtga aataccacca ttccgttcgg aaggagggat gattcccatg gtcctggggg
      301 aagtgccagt tccatggatc cagagccgga cgccagtccg cggatccggc ggccggcgcc
      361 gtcgtgatca gcaggacgag gaacaggctc agggcgcggc agaatggtga ataattgtcc
      421 attttcctct tcgtcttcgt cccggccggc ggcgctgccg ggtatggatt tccccggttc
      481 ctcattcggc acggatctgc atctgcgggt aggtgcgcag aaccatgacg atgggataca
      541 gcgccgcccc caggcttgcc aagaccgctg tcgccgccac gacgagatag ggcgaaagct
      601 gggggtgaaa aaagatcgtc cagtggaagc tccgcagatt gatcacccgg atgagcacca
      661 ccgcgaagag agttcccacc ccggcgctga ggagaaagct catcaggccc atgccaacgg
      721 cctccagaag agtcatgagc gatacctggc cggtggagaa gcccagagcc cgcaggattc
      781 ccagctcgct ttgcctttcg atgaagaggg tcatgagggc gccggcaatg ccgaaaaagg
      841 caataatgat ggccatgatc cgcatggaac gggtgatcgc gaaggtgttg tcgaagatcg
      901 ccaggatgtt tccgaagaac tcctcccggg tgaagacggg gagatcgtat gctccggccc
      961 tctggcgcac caggtccagg gtctgccggc gccggggatg gtcgggttcg aggaagacgg
     1021 ccacgctgtt gatggtggga tcggcgaaga gtttcaggta ggtatgtcga tccatcatca
     1081 ccaggccgtg ctcggtggaa taatcgtaga agactgcttc cacccgcagc tccgccgggc
     1141 cgttccttcc ctccagggtg atggaatctc cgagtccgat ggcgaaattc cgggcaaagc
     1201 tttccgagac gatgaccgct cccctcttta ccgcctccca gtgctcgtct ccgccggcga
     1261 gccaggcaaa gcgtgtgtaa cgctggagga ccggggcatt gacggcggcg acaaacatgg
     1321 gtttgttccg gtaggtgatc tggaccttgc gataggcgtc caccccggcg atatcccgca
     1381 tctccagcaa ctcgtggtag aagccctctg gaacctcctt gtccgtggcc gtggagatgt
     1441 aaagatcgcc ccgcaactgt cctgacatcc accagacaag ggactcacga aaactgccga
     1501 tcatgagggc catgcctatg gacagggaaa gggccaccat gaatgccgcc acggcgatcg
     1561 ccgtgcgtcc cagattcctc ctgatgttgc cggcggcgat ctttcccgtg atgcccccca
     1621 ggcaggtcag tgccgcccgg atcgggggtg tgcaggccac caccgcgtat ccggtcatga
     1681 ggctcaggcc gaataccatg ccgaagaggg cgccaaaggc gatataaatt gacccatcgg
     1741 gcaggagaaa aagggaggcg ctcagcaggg ttattcccag accgatagcc gctgcttttc
     1801 ttgccgccgg tcctccccgg cggttcatgg cgcgcccgga aagggacttt acgggatcgg
     1861 tgcgcgccag ttccaccaac gggtagatgc ttcccaggat gctcgcgccg cagccgagaa
     1921 ggatgccgac gccaagcatc tggaaagacc agggcagcgg caggggatgg agaaagaaat
     1981 agagattgct taccgtgcca gcgatgatcc cgctcaggaa acgactcagc aggtacccca
     2041 tgacggaacc caaggcgcct ccgaccaggc cgaggatgag gatctctgtc atgaacgcca
     2101 gaatgacctc gccgcggctg gcgcccaggc tgcggaggat gccggcgtcg cgcctgcggc
     2161 tgactacggc gaacatggtg gtattataga tgaggaatac agccacgaaa agcgccatga
     2221 gggaaagggc ttcgaggttg atccggaagg cgccgagcat ggcgcgcagg gtgtcgtggt
     2281 tttgccggtt ggaacgaatc cggaatccct gccgccagcg gctcaggaaa ccctcctcgt
     2341 ccgtcaggat caggtcgata cgatcgatgc gtccggggag caaaaaaatc tcctgcgcat
     2401 gggcgatgtc cataagcagg aggggttccc cggaggggtt ggggaaggtg ccgatgacct
     2461 cgatctcccc ctgtgaggtc cgtaatctgc ccccggccgc aagacccaca gcggcggcga
     2521 tgtttgcctc catcaggacc gcccttgctt cgagcaggaa cgacatccct tcccgggcgt
     2581 cccggtgcgg cgcgccggtt atggaacggc gggacagttc gggacggatg gccgcatcga
     2641 gaaacggatc gataccgagg agccggatct gggcgccatc cccgtaccgg agacgccgat
     2701 cgatgaccgg cgcgaaggaa tgcaccgccg gatcctgcat gatttccttg aggatctgtt
     2761 catccatggg accggcatgg cgttccagcg tatgggtgga tttgccctgg agaaattcga
     2821 tggcgccggt aaagcttgcc agggccgaca gcgcggagag ccccatgccc agtgcggccg
     2881 ccaccccgaa ggcaatgccc attagttgga gcagcgtcag gcttttccgc ctgacaaggt
     2941 atcggagaaa tgactttgtt accgtgccca tgactggttt accgtgcggt caccagcgcg
     3001 gtgccggggc atgcatcggt gctccgcctg catccgcggc ggcgatcacc ccatcgatca
     3061 tggacaggac ccggtctgcg aatccggttg ccgtggggct gtgagaaacc atcaacagcg
     3121 acatgcccga ctcccggcag cgggtggtca gcatttcgag aatctgtctg cccgttgtgg
     3181 tatccagatt gccggtgggc tcatcggcaa gaatcaacgc tggtttcttg accacggccc
     3241 gggcgatagc cacccgctgc tgctccccgc cggagagttc atgggggtac cggcgttcct
     3301 tgccctccag accgagtttt cccagggctt cacgggcgtt ttcagggctg gaaatgccgg
     3361 cgagttccag gggcagatag acattttcaa gtaccgtcag agtcggcagg agattgaaga
     3421 actggaagac gaagcccagg cggttccggc gcagcaaggt gcggcctttt tcccccagac
     3481 tttcaaggtt ttggccttcc acttcgatcg tgccgcggct ggggcgatcc agaccggcca
     3541 ggagattcag gagggtcgtt ttccccgatc cacttttgcc gatgagcgcc acgacttcgc
     3601 ctcgggcgat ctccatgcca accccccgga gggcggggtt gtgatcgtag atcttccata
     3661 aatcgcgagc gcgaattacc gggacgttca aaccatgctc cttttaccga tgtttgtcga
     3721 gaaacggcag cggcttgccc gttcgataga cgagggcctg acaggaagcg ataagccggt
     3781 ccgatttttc ccgcaccttg atgtcgtaaa cggccgttct gacggtgcgg tttatttccc
     3841 tggcttcggc gaccaatatg gagccggaac ttggggaggc gaggaatccg atgctcatat
     3901 tgagggccac ggcggtcgtg ccgtgagaat tggaagcggt ttcgaacgcc tcgtcgatga
     3961 gggcgaacag cgccccgccg tgagccttcc cgaaaatatt ctccatgtcc ggagtgaacg
     4021 ccatctcaac tcgggcataa ccctcgtcga gctccactag ttgaagatgg aatttccggg
     4081 cgaagggttc ctgtgccacc tgccggaaga tcgcttccct gattgtcgaa tccatttttg
     4141 tcccgtctga tattttgccc tttattactc tatgttgtcg tcaagctgcg gggcgagtat
     4201 aaagcaaatg ccactggagg tcaaaagaaa agcccctgcc gcggtcggcg gcaggggccc
     4261 ggttctggag ggtgaggggg gatggcggct tacggaacgt tgcccctttt caagtgctcg
     4321 atgatgtagg cctcatcttc cttcaattca cgaacctctt tcttttcttt tgccttttgc
     4381 atgatttgtt ctcctttctc tatccatcat ggccgtttgg gtccggctaa ttttacaggc
     4441 gggaatagca tggttcctgc ctcctctttg ttctttcgat ggcaggataa tcccggaaga
     4501 ttttttacgc catcggggtg aggacgaaag tggggtgaga aaacggcgtt aatatggctc
     4561 aggatgccgc tccccggcac tcggtatttg tccgatgtgg gacgggagag ccgcgtttgc
     4621 ggcggaaaac gaggttgcca aaaggaaagg ggatggttta atataacccc cggaccgggc
     4681 aatcaaattc ttatgggagg cggaacatgg agttcatcag tgtcatggag gcgaggaggg
     4741 ccgtcaattt tttcgatcct gaaaagccgg taagcgacga tgagttgaga aagatcgtag
     4801 aaaccgccgc ccttgccccc tcgggattga attttcagcc ctggcaggtc attgtcgtgc
     4861 gttcgccggg agcgaaggag aagttgatgc tggcggcctc caagcaggtc aagattatcg
     4921 aggccccggt gaccctcatt gtcctcgccg atcgggacgg ctggaaggca gggcatccaa
     4981 cggtcgaaaa ggtgtgggat aatctcgtcc ggctcggtta ctggaacacc gggcagcgcg
     5041 gggggtttga aaaaggtccg gagaaattat acggcactcc ggagcgatca ctggcctttg
     5101 ccgtgaagaa caccgccttt ttcagcatgg cgctgatgct ggcggcaaag gatgcgggcc
     5161 tcgactgcca tcccatggac ggattcgatc atgaggcggt aaaacgggcc ttcgatgttc
     5221 cggacaattt cttcatcccc ctgttgatcg ccatcggtca tttcgacgcc cnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nacgtcgtaa tcggtcaggg ccgggaaact
     5401 catgggatcc acccagatcc cgcccacctt ccgggcccgc ggcccggcgg cagtatcccc
     5461 gcccccgttt accgtcagaa agcgcttcct cttcatggac tgcttacctt tccgttcctg
     5521 tccgcgctac cggcgcggtc tcctgttcct ttcgaccatc cagcccagga ggcctttctg
     5581 gggattgaac tcgactcttt catctcttgt catggtcatg ttcgcacctc cttcggttat
     5641 ctccttcctt ctttcgatca tagggtaagg caggacgtgt tttttcacaa tcggtgatgg
     5701 gatgaataag cgatgggatg ggggaggatt gtccggtagg ggataccgga tggcaatcat
     5761 cggaaattct ccgatagcca gaaaggatgc ctgagaaaca aagccgctcg cgtcatcatc
     5821 gatatatttt cccgttgttt ggatgacggg agcggccaga atgattcagc ttttttccat
     5881 gtgcatttgg gctgtcctaa cggccagatc tctatccttc gtgaacatcc accactgtgg
     5941 ttgggacgta tgggggccgt tgtccttttc gcgaccccgg gaaaggtacc agttcccata
     6001 acgctcaacg gtggcaaagg gatggtcgcc gtcacagagg atgctgcact ccctgatctc
     6061 gttatagaat attgtttcct cagggtcttc ttctctggac agctcgtcgg gaaggacgat
     6121 gtgtgcaacg tctttgcctg ttgcttcctg gctgtccagc cattcggaaa tggtcatctt
     6181 atctctcttc tcttaagtta ctgcatgata aggcgcccca gggggcatgg atcaatcact
     6241 atgtctggag aagaattttc ataaaaatcc gattgttgac cgagtcaaat cgattgcatc
     6301 cgatcgtccg acatgggcaa tgctaagata ttgcctaatg atttgaactg ttgagagcgg
     6361 tgatttgatc attcagcgtc cagtagtcgt aaatgtatcc gataccgaaa atccctgcgg
     6421 agcataggta aaggatgccg gttatccatt tccccatata gaaacgatga attccgaaca
     6481 ggcctaaaaa ggtcagcagc aaccaggcga tgttgtaatc gatccggcct tcggtaaatc
     6541 tcaactcggc tttgcgcgcc atggaaggga tgagaaagat gtctatcaac catccgatcc
     6601 ccaatagtcc cagggtgaaa aagtaaatgg ttcctgaaac aggttttcca taataaaaac
     6661 ggtgcgagcc ggtaaatcca aatatccaca gaatatagcc aatcagaacg ctatgggttt
     6721 gatgacgctg ttccattagt acacggtcct tcctaactga ttcagccccg caaatttaac
     6781 gacatcgcca aaggtgaaaa gacatgagtt ttgagaatca gctctggaca ttgcccggtg
     6841 ataatcacgg gccgttcacc atgggaaatt gcgcttcggt atggcattgt tgatgcggat
     6901 gataccgggg atgcggttga cccataggca ggcagtccaa aaagagttat ccaaaacgct
     6961 tacaaaatac cctaaaataa cgggggagtt aacggacgta ccagctaacc ccccctgcta
     7021 ctctttatgg tgccgaagcc gggacttgaa
//
LOCUS       DAOSMF010000095         7041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_11829_length_7041_cov_22.370169, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000095 DAOSMF010000000
VERSION     DAOSMF010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 7041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7041
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11829_length_7041_cov_22.370169"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1612
                     /locus_tag="PLT63_08595"
     CDS             <1..1612
                     /locus_tag="PLT63_08595"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOD98821.1"
                     /translation="EGRVDLIPSRFSRIPWLFRTGAIHIDIAFIQITPPDETGYCSLG
                     LGMSVERQAMESAAIVVGEINPRAPRTLGDTLVHIDEFDALIEGTERFFYVPRWPLDE
                     VYLKIAGHVATIIEDGSCISFAIGPLYEALGSQLKGKRNLGIHSPFFTDALMELVKSG
                     AVSNRRKVVFRGKSVASYILGTEELLTWLDRNPLVEFQPIDVVLNPKVIGRNDRVMAI
                     LPARKVDLTGNVALHMGKGNVTSGPGAVQELFMGAAMSAKGRTVFALPSRNRQGEANI
                     VVSVAAMPYQFSNRESLDIVVTEYGVAYLMGRTLRERALALIDIAHPDDRAELFQQAK
                     QAKMIYADQIYFAESGHLYPEKVSGAHTFKDGLSVRFRAIKPSDEDEMRRLFYRFSDQ
                     AVYYRYFSPIKAMPHKKMQEYVNVDYRRIMSIVGVIEDSGRERIIAEARYVRAPERPW
                     ADTAFIVDETFQGRGIASFLFSLLAKNAREEGIEVFTADVLAGNKAMLRVYEKADFPI
                     KAVLQSGIYELTILLLEKASDNVKEAVP"
     gene            1713..1799
                     /locus_tag="PLT63_08600"
     tRNA            1713..1799
                     /locus_tag="PLT63_08600"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1747..1749,aa:Leu,seq:tag)
     gene            1850..>2841
                     /gene="tig"
                     /locus_tag="PLT63_08605"
     CDS             1850..>2841
                     /gene="tig"
                     /locus_tag="PLT63_08605"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOD98822.1"
                     /translation="MSEGNQLVTIEEISPVKKKLNFEIPWEETRKSLDNAYRIIAKTA
                     KVKGFRPGKIPRSILERYYKEYAEEETITDIFNRYFWDALKEHDIEVIARPEVDQQGI
                     TAGESFSFSATVEVEPDFDPEGYLGLEIDREEVEVTEDDIAAKLAELQEMYSTMEETD
                     EESEVAKGRYVVIDFQGVLDGQPLKEMKADDYLLEIGSQSFIPGFEDELVGMKKGERK
                     EFTITFPDGYHHQAVAGKEVVFSVQLKNIKEKRLPEINEEFVKNFEEFNSLEELKADL
                     RVKIEAQKTEKADGDMRIAMMDRLLEKNPFPAPETLVERQILSMMADTKWRMAM"
     assembly_gap    2842..2941
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2942..3145
                     /locus_tag="PLT63_08610"
     CDS             <2942..3145
                     /locus_tag="PLT63_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HOD98823.1"
                     /translation="GFQGQATDIDIQAREILRLKDELNRIMAAMTGQPLAKIQADTER
                     DYFMSGQQAREYGIIDVIIEKRQ"
     gene            3171..4436
                     /gene="clpX"
                     /locus_tag="PLT63_08615"
     CDS             3171..4436
                     /gene="clpX"
                     /locus_tag="PLT63_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOD98824.1"
                     /translation="MVRKGRDTAGGGPGILYCSFCGKNQNEVRKLVAGPTAYICDECI
                     ELCRCIVQEDGEAPPALDKAKGTPKPKEIKKFLDSYVIGQERTKKILSVAVYNHYKRL
                     FRPADLEGVEIQKSNVLLIGPTGSGKTLLAKTLAKMLNVPFTIADATTLTEAGYVGED
                     VENIILSLLQNADYDVERASKGIVYIDEIDKIARKSGNPSITRDVSGEGVQQALLKII
                     EGTMANIPPKGGRKHPQQEFIQVDTTNILFICGGAFNILEDIIAKRVGFSNMGFGAEI
                     RSKQDRKVGELLTEVQPEDLLKFGLIPEFIGRLPVIATLNELNEEDMVRILKEPRDSI
                     IKQYKQLFDLENVKLKFTDGALRAVAKLAIERKTGARGLRSILENTMLEIMYELPSRR
                     DMRECVISEEVVLNRENPILIFESASETA"
     gene            4468..6900
                     /gene="lon"
                     /locus_tag="PLT63_08620"
     CDS             4468..6900
                     /gene="lon"
                     /locus_tag="PLT63_08620"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOD98825.1"
                     /translation="MPEYLKNKEIGKITVMPLLPLRDVVVFPHMIVPLFVGREKSIAA
                     LESAMKYEKSIFMVAQKNAQKDEPTESDIYRVGTIGIIIQLLRLPDGTVKVLVEGKER
                     GVIKEFNTGEDFFSVEVEEVVEDDESYGDHVKSEALMRSLNESFVSYVKLGKKVHMEM
                     INTVSTIESPSKLADVIVSHVGVKLEEKQKILEILNVSERLEAVYELLLGEIEILQVE
                     EKIKRRVKKQMEKTQKDYYLNEQMRAIQKEMGEKDDLKSEIAELEKRLKAKKMPEEAY
                     KKVKAEIKKMQMMAPMSAEATVVRNYIDWLLDLPWYEKTENKYALKESEAILEEDHFG
                     LKQVKERIIEYLAVQSLIKKNKGSILCLVGPPGVGKTSVAKSVARATNRKFVRFSLGG
                     VRDEAEIRGHRRTYIGALPGKIIQLMKKAGTSNPVLCLDEVDKLSSDFRGDPSSALLE
                     VLDPEQNNAFNDNYLEVDYDLSDVMFITTANVLQTIPAPLQDRMEVIRIAGYTEPEKL
                     CIARRFLVAKEMEANGLTEKNIDFSDGAILAIIRKYTREAGVRNMEREIASICRKVAR
                     KIVAEGIQEQIRVTSKSVAKYLGPPKYRHGESEGKAQIGLTTGLAWTEVGGELLVIEA
                     TIMKGTGKMVMTGKLGDVMQESVQAAMTYVRSRADEFSLPDNYYKETDVHVHVPEGAI
                     PKDGPSAGIAMATSIASAYMKKKVRGDLAMTGEITLRGRILPIGGVKEKLLAAHRGNM
                     KIVIIPKENEKDLAEVPANVLKAMKIVFVEHMDDVLAAAFAEDDDQTGKEAEAVPAVD
                     SLTSSPAAAYVN"
BASE COUNT         1729 a         1780 c         2085 g         1347 t
ORIGIN      
        1 ggagggaagg gtggatctca tccccagccg tttttcccgg atcccctggc ttttccgcac
       61 cggggccatc catatcgata tcgccttcat ccagatcaca ccgcccgatg aaacgggcta
      121 ctgcagcctg gggctcggca tgtccgtgga gcgccaggcc atggagagcg ccgcgatagt
      181 cgtcggtgaa atcaatcccc gggccccccg caccctcggc gacaccctgg tccacatcga
      241 tgagttcgac gccctcatcg aaggtacgga acggttcttc tatgtccccc ggtggcccct
      301 ggacgaggtc tatctgaaaa tcgccgggca tgtggccacc attatcgaag acgggagctg
      361 catttccttc gccatcggcc cgctctacga ggccttgggc agccagttga aaggcaagag
      421 gaatctgggc atccattccc cattctttac cgacgccctg atggagctgg tgaaaagcgg
      481 cgccgtgtcc aatcggcgca aggtcgtctt tcgggggaaa tcagtcgcct cctatatcct
      541 gggtacggag gagcttctga cctggctgga ccggaatccc ctggtggaat ttcagcccat
      601 tgatgtggtc ctgaatccca aggtcattgg acgcaatgac cgggtgatgg cgatcctgcc
      661 tgcccgcaag gtggacctga ccggcaatgt ggctctccac atgggcaagg gaaacgtcac
      721 ctccggcccc ggcgcggtcc aggagctttt catgggggcc gccatgtccg ccaagggaag
      781 aacggtcttt gccctgccga gccgcaatcg ccagggcgaa gccaacatcg tcgtttccgt
      841 ggccgcgatg ccctaccagt tttccaaccg cgaatctctc gacatcgtcg ttacggaata
      901 cggagtggcc tacctgatgg gaagaaccct ccgggagcgg gccttggccc tgatcgatat
      961 cgcccatccc gacgaccggg cggagctttt ccaacaggcg aaacaggcca agatgatcta
     1021 tgccgaccag atatatttcg ccgaatccgg ccatctgtat ccggaaaagg taagcggcgc
     1081 ccacaccttc aaggacggat tatcggtccg tttccgggcg atcaagccgt ccgacgaaga
     1141 cgagatgcga cgcctctttt accggttttc cgatcaggcg gtctattatc gctatttcag
     1201 ccccatcaag gcgatgcccc acaaaaagat gcaggagtat gtcaacgtcg attaccgacg
     1261 tattatgtcc attgtgggtg tcatcgagga ttcgggacgg gagcggatca tcgccgaagc
     1321 ccgctatgtc cgcgccccgg agcgcccctg ggccgatacg gccttcatcg tcgatgaaac
     1381 ctttcagggc aggggaattg cctcatttct cttttccctg ctggcgaaaa acgcccggga
     1441 ggagggcatc gaggtcttca ccgccgacgt gctggcgggc aacaaggcga tgctgcgggt
     1501 ctatgagaag gcggattttc ccatcaaagc tgtcctgcag tcggggatat acgagttgac
     1561 cattctcctc ctggaaaagg cgtcggataa tgtgaaggaa gctgtcccct gaaaaagctt
     1621 gcaaaaggca agggtttggg ttaaagcctc gcttcttttt ggatattgtt gacaatcttt
     1681 tcgtcaatgc atatatagtt ctgggtcaat cagcgagagt ggcggaattg gcagacgcac
     1741 tggacttagg atccagcccg cgtaagcggt taggggttca agtcccctct ctcgcaccaa
     1801 ttgaccctgc caggaccaac atcacgaggt gaaggagaag agaagaacgg tgagcgaagg
     1861 aaaccaactg gtaacgatcg aggaaattag tccggtaaag aagaagctga actttgaaat
     1921 cccctgggag gagaccagga aatccctgga caatgcctat cggataatcg ccaaaaccgc
     1981 caaggttaag gggttccgac cggggaagat accccggtcc atcctggaga gatattataa
     2041 ggaatacgca gaggaagaaa cgataaccga tattttcaat cgctatttct gggatgcatt
     2101 gaaagaacac gacatcgagg tcattgctag gcccgaggtc gatcagcagg gtatcaccgc
     2161 gggagaatcc ttttccttca gcgctacggt ggaggtcgaa ccggacttcg atccggaagg
     2221 ttacctcggc ctggaaatcg accgggaaga ggtggaggtg accgaagacg atatcgccgc
     2281 caaactagcc gaattgcagg agatgtacag cacgatggag gaaaccgacg aggagtcgga
     2341 ggtcgcaaag ggccggtacg tcgtcatcga ttttcagggc gtcctggacg gccagcctct
     2401 gaaggaaatg aaagccgacg attatctcct ggagatcggt tcgcagagct tcatccccgg
     2461 tttcgaggat gagctggtgg ggatgaagaa gggggaacgc aaggaattca ccataacatt
     2521 tcccgatggt taccaccacc aggcggtggc gggcaaggag gtcgtattct cggtgcagct
     2581 caagaacatc aaagagaaga ggcttcccga gattaacgag gaatttgtca agaatttcga
     2641 ggaattcaac agtcttgaag aattgaaggc ggatctgcgg gttaagatag aggcccagaa
     2701 aacggagaag gccgatggag atatgaggat tgccatgatg gaccggctgt tggagaagaa
     2761 tccctttccc gcgccggaaa ccctggtgga aaggcagatc ctttccatga tggccgatac
     2821 gaaatggcgc atggcgatgc annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nggatttcag ggacaggcca cggatatcga tattcaagcc cgggagatcc tcaggctcaa
     3001 ggacgagttg aaccggatta tggctgccat gaccggtcag cccctggcca agatccaggc
     3061 cgacacggaa cgggactatt tcatgagtgg gcaacaggcc cgagaatacg ggatcatcga
     3121 tgtcatcatc gagaagcgcc aataaaacaa taatcatgag gtcagtgtaa atggtcagaa
     3181 aaggtagaga tacggcgggc ggcggcccgg gaatcctgta ctgttctttc tgcggcaaga
     3241 accagaacga ggtgcgaaaa ctcgtggccg gaccgacggc atacatttgt gacgagtgca
     3301 tcgagctgtg ccggtgcatc gtccaggagg acggagaggc gccgccggct ctggataagg
     3361 ccaagggtac ccccaagccc aaggaaataa agaaatttct cgactcttac gtgatcggtc
     3421 aggaacgaac taaaaagatt ttgtccgtag ccgtttacaa tcattacaaa cgcctgttcc
     3481 ggcccgccga cctggagggg gtggagattc agaagagcaa tgtcctgttg atcggaccca
     3541 cgggttccgg aaagaccctg ttggcgaaga ccctggccaa gatgctcaac gtgccgttca
     3601 ccatcgccga tgccaccacc cttaccgagg ccggctatgt gggtgaagat gtggaaaaca
     3661 tcatcctgtc gctgctgcag aacgccgatt acgacgtgga gcgggcgtcc aaggggatcg
     3721 tttacatcga cgagatcgat aagatcgccc gcaagtcggg caatccttcc attacccggg
     3781 acgtgtccgg ggaaggagtc cagcaggccc tgttgaagat catcgagggg accatggcca
     3841 acatccctcc caagggaggg cgaaagcacc cccaacagga attcatccag gtagacacaa
     3901 ccaacatcct gttcatctgc gggggcgcgt tcaacatcct cgaagacatc atcgccaagc
     3961 gggtaggatt cagcaatatg ggtttcggag cggaaatcag aagcaaacag gatcggaagg
     4021 tgggcgaact tctcacggag gtgcagcccg aggacctctt gaagttcggt cttattccgg
     4081 aatttatcgg ccgcctgccc gttatcgcca ccctcaatga actcaatgaa gaggatatgg
     4141 tacgcatcct aaaggaaccc cgtgactcca tcatcaagca atacaaacag ttgttcgatt
     4201 tggagaacgt gaaactcaaa ttcaccgacg gcgccctccg ggcggtggcg aagctggcca
     4261 tagagagaaa gacgggggcc cggggattgc ggtccattct ggaaaatacc atgctggaga
     4321 tcatgtatga actgccctcc cgccgagata tgcgggagtg tgtcatcagc gaagaggtgg
     4381 ttttgaaccg ggagaacccg attctgatct tcgaatcggc ttcggaaacc gcctgagaaa
     4441 gctacataaa ggaaaatatc atattatatg cctgagtact tgaaaaataa agaaataggt
     4501 aaaataacgg ttatgccatt gctgccgttg cgggacgtgg tggttttccc ccacatgatc
     4561 gtcccgctct tcgtcggccg ggaaaaatcc attgccgcct tggagtcggc catgaagtat
     4621 gaaaagagca tcttcatggt ggcgcagaaa aacgctcaga aggacgaacc gacggaaagc
     4681 gatatctata gggtcgggac gatcgggatc atcatccagt tgctccggct gcccgacggg
     4741 acggtgaagg tcctggtgga aggaaaagag cggggggtca tcaaggagtt caacaccggg
     4801 gaggattttt tttcggtgga agtggaggag gtcgtggagg atgacgaatc ctacggcgat
     4861 catgtcaaat ccgaggcctt gatgcgcagc ctcaacgagt ccttcgtaag ctatgtaaag
     4921 cttggcaaga aggttcacat ggagatgatc aacaccgtat ccactatcga gagcccctct
     4981 aagctggccg atgtgatcgt ttcccatgtg ggggtaaagc tggaagaaaa gcagaagata
     5041 ctggaaatat tgaatgtcag tgagcggctg gaggccgttt acgaactgct gcttggtgag
     5101 atcgagatcc tccaggtgga ggagaagatc aagcgccgcg tgaagaagca gatggagaag
     5161 acccagaagg attactatct gaacgaacag atgcgggcta tccagaagga aatgggggaa
     5221 aaggatgatc tcaagagtga gatcgccgaa ctggagaaac ggctcaaggc gaaaaagatg
     5281 cctgaggagg cctacaagaa ggtcaaggcg gagatcaaga agatgcagat gatggccccc
     5341 atgtccgccg aggccacggt ggtgcgaaac tacatcgact ggcttctcga tctgccctgg
     5401 tacgagaaga cggagaacaa gtacgcccta aaggaatcgg aagcgattct ggaagaggac
     5461 cacttcggcc tgaagcaagt caaggagcgg atcatcgagt atctggccgt tcagagcctc
     5521 atcaaaaaga acaagggttc gatcctgtgt ctcgtgggtc cgccgggggt gggaaagacc
     5581 tcggtggcga agtcggtggc ccgggccacg aataggaagt ttgtccgctt ttccctgggc
     5641 ggggtccggg acgaagctga gattcgcggt caccgccgca cctatatcgg cgccctgccg
     5701 ggaaagataa tccagttgat gaaaaaggca ggaaccagca atcccgtcct ctgcctagac
     5761 gaagtggaca aactgagttc cgatttccgc ggcgacccct cgtcggccct cctcgaggtc
     5821 ctggacccgg aacagaacaa cgccttcaac gacaattacc tggaagtgga ttacgatctg
     5881 tcggacgtca tgttcataac cacggccaat gtcctccaga cgatcccggc gccgttgcag
     5941 gaccggatgg aagtgatacg catcgccggt tatacggaac cggagaagct ttgcatcgcc
     6001 cggcgctttc tggtggccaa ggaaatggag gccaacggtc tcacggagaa gaatatcgat
     6061 ttttccgacg gggcgatcct ggccatcatc aggaagtaca cccgcgaggc gggggtacgg
     6121 aatatggaaa gggaaatcgc ctccatttgt cggaaggtgg cccgaaagat cgtggcggag
     6181 ggtattcagg aacagattcg ggtaacatcc aagtcggtgg ccaaatacct ggggccgccg
     6241 aaataccgtc acggcgaatc ggaaggcaag gcacagatcg gcctcaccac cggcctggca
     6301 tggacggagg tcggcggcga attgctggtt attgaggcga cgatcatgaa gggaaccgga
     6361 aagatggtca tgacgggcaa gcttggcgat gtcatgcagg aatccgtcca ggccgccatg
     6421 acctatgtcc gctcccgggc cgatgaattc agtctgccgg acaattatta caaagagacg
     6481 gatgttcacg tccacgtacc cgaaggcgcc attcccaagg atggaccatc ggcggggatc
     6541 gcgatggcca cctccatcgc ctcggcgtac atgaagaaaa aggtccgggg ggacctggcg
     6601 atgaccggcg agatcaccct gcggggcagg atcctgccta ttggcggggt caaggaaaaa
     6661 cttttggccg cccatcgggg gaacatgaag attgtgatca tccctaagga aaacgaaaaa
     6721 gatctggccg aggtgccggc caatgtcctc aaggccatga agatcgtctt tgtggagcac
     6781 atggatgacg ttctggcggc ggcctttgcc gaggatgatg accagacggg aaaggaggcg
     6841 gaggcggtgc cggcggtgga ttctctgacc agctcacccg ccgctgccta tgtaaattga
     6901 tgatttcttt ttccttgaca agcatggaca atgttgatag tttgccgatc ttacttgagg
     6961 cgacagcaag gggcgggtag ctcagatggg agagcgccac gcttacaccg tggatgtcac
     7021 aggttcgagt cctgttccgc c
//
LOCUS       DAOSMF010000096         7019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_11885_length_7019_cov_12.510339, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000096 DAOSMF010000000
VERSION     DAOSMF010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 7019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7019
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11885_length_7019_cov_12.510339"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(663..3014)
                     /locus_tag="PLT63_08625"
     CDS             complement(663..3014)
                     /locus_tag="PLT63_08625"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOD98826.1"
                     /translation="MISKTVRVFFVLVMFFLLTPSLSPAATERRTALVIGNSAYSSGP
                     LKNPVNDATAMDAQLKKLGFTVILKKNANLKGMETALTEFGDRLKRGGVGLFFYAGHG
                     LQVNGVNYLVPIGAKINRESDIKYETMDAGKILDEMANANNGLNIVILDACRDNPFAR
                     SFRNAVRGLAIVSNAPVGTFISYSTSPGKVARDGDGRNSPYTKALLKYMKEPGLSIEH
                     MFKKVRTSLGKNTGGKQIPWELSSLQGDFSFVPSATATLSTVSKKTTVSDTMNTKDIK
                     IATIQPPGATEKKYTDDNCGRMMERGDQYAEKALWGNALIQYQKAETLKCKHPEWFNK
                     MLEAKDNINRRVKKAIAVFDFGSPSNEKDAGKIAAVKLLAYLHRNASGDIRLIERENL
                     MSIIREMQLGKANVADIKTAQSVGKVRGIDIFIMGDVLHFSTKTMDNPSVSQAKVLVS
                     EEGTKKNYQYIPYKHGTAKIIAAIEISYKLVDTKTGENIFSDTITGKLIKEDKYSDGV
                     PEGNVRHDPLQLPTEVEVLDELANAKISEMGRSILRHFQSIEVEYFNQAQQFQKRRNF
                     DEAIEKYTDAVFDEKLKGISTPISQAAHKMIDKLTREKTGEEPVNEVKNEKYVTDVVD
                     RKAIALAAPSIMSSQKSTKLKVSVFQFGTINLEAAGYGSTVTNLLKQSLSKDPVLSLI
                     NQRELMSFLSLNDLQQNEQMDNVLNIGARLGLDVIVVGNVEKNGAAIIINCKVIHIKK
                     QIAILSVRVSSRGDSALINEINKLSSSISRTISESGLVAKTNG"
     gene            complement(3058..5970)
                     /locus_tag="PLT63_08630"
     CDS             complement(3058..5970)
                     /locus_tag="PLT63_08630"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOD98827.1"
                     /translation="MRRLILGLGIIVIFCLMLLGPINAPVTAAEPPKEPILKIETAMH
                     TAVIKRIGVDDATRYLVTASDDKSIRVWELATGRLLSVLRPPIGEGDEGKIYAVAISP
                     DGQTVAAGGWTQFAGPQGGLADDGVSIYLFHRPTGELTRRITGLPSVINHLAFSPDGR
                     YLAATLGQNNGIRVFRAADGRPVGEDRDYGTDSYSVVFDRRGRLAAASLDGYIRLYDR
                     PGAGGLKLLARQKAPGGKQPFSIFFSPDGEKLAVGFNDSTRVDVLSGQDLSHLYSPDT
                     SGVDNGDLSKVSFSPDGRLYAGGTYQKGGQFPILSWGKESRGEHRELPGSPNTIMSIL
                     PLAAGRVVYGAGDPAFGVLDKNGQRILFQSAQIADYRANCEGFRLSSDGKKVSFGYEL
                     WGKSPATFDLSTRTLSAGGEAAGLYPPDTTGLPVTDWKNSRSPKLNGRALALRQYEMS
                     RSLAIAPSRDSFLLGTDWYLRYFDRKGKEKWQALVPGAAWAVNISKDGRLGVAAFGDG
                     TIRWYRLTDGKELLAFFPHKDRKRWVLWTPEGFFDASEGGAELIGYHLNRGKDQAARF
                     VNVDKLYDQFYRPDLVLAKFQGKDISEYAKAVDVNRLLTTEALPPSVRLTTASGPRKS
                     RDTEIAGEICDQGGGIGEVTFFLNGMPVVVDTDGRSLKVVSQGKGSGQPLACKSFRHR
                     VTLAAGENVIGLMARNKDNTIESNRAEAKLTFRSGGKEKPDLYVLAVAVNKYRDGDLR
                     LKYPIPDANDLVRSIQGGGKRLFGKVVVKTLYDDDATRAGLEKAFRELEDKTRRDDVF
                     VLFLAGHGITYEKDGNYYFLPVDFRFTGEEAIKAQGVSKDDLMRNLTHIQALKSLLLL
                     DTCNSGSFAEAVASRNIVEKTAVARLSRATGRSTIVASSKDQVALEGYEGHGVFTYTL
                     LQGMSGAAKDREGKVTVNGLATYVEETLPKITYKKWGYEQIPQKSLQGMDFPLALQ"
BASE COUNT         1569 a         1925 c         1734 g         1791 t
ORIGIN      
        1 aagattcctt gcctcgcggc gctttcgtgc cggtgcaatt actcacaccg cgaccggagt
       61 gacggccctt caaatccggt gtggggcgct aaaaccgtcc caggtgggtt cgattcccat
      121 gcacttccgc cattttatta acaaatttcg ctttttagcg aaacaatttc tttaacctgt
      181 cttgaacctt ctttaaccct atcagaatca gcgattttga aagaatcggg cggtattgcg
      241 tataacttct tgaagtcaat aagaattaag tttccctttt tatgcaacct tttcgatccg
      301 aaatattgcg gatcatctct atgggggagc cggaatattt ctgggatgac tatatccagg
      361 cgtaacgctt cgagaatcgg aagaatcagc gaaagatgat ttgtggcgtg caggagaagc
      421 ttgttttaat gatgtccggt acggcctacg tgactggaaa acgatcaaaa aaagggcaga
      481 tggtcaaagt tccccagcct atgggttgtc tctacacgtt tttcctacaa tcatcagatc
      541 cgtatcaccc atttttcacc aatttcccct tgacatgcaa gttccccatg tcttatagtc
      601 cgatcatcaa aaagcaatgt tctatcaatt caataatatt tccactacat tcccacattt
      661 tctcagccat ttgttttcgc cacaagaccc gattccgata tagtccttga gatcgacgaa
      721 ctaagcttgt tgatttcatt aatcaaagcg gaatcaccac gggaacttac ccgtacactc
      781 aagatggcta tttgtttctt gatatgtatt actttacaat taataataat ggctgcgcca
      841 ttcttctcaa cattaccgac aacaatgaca tccaagccca atctcgcacc tatgtttaaa
      901 acattatcca tctgttcgtt ttgttgaagg tcattgaggc taaggaagga catgagttcc
      961 ctttgattta ttagtgataa tacgggatct ttcgataagg attgcttaag tagattcgtt
     1021 accgttgaac cgtatcccgc agcctctagg tttatcgttc caaactggaa tacggagact
     1081 ttaagttttg tcgatttttg agacgacata atggacggcg ccgccaaggc aattgccttt
     1141 ctgtccacca catctgtaac atatttttca ttttttactt catttacagg ttcttcgcct
     1201 gtcttttccc gagttagttt atcgatcatc ttgtgtgctg cctgcgaaat cggtgtcgag
     1261 attcccttga gcttctcatc aaacacggca tctgtgtact tttcgatcgc ctcatcaaaa
     1321 tttcgcctct tctgaaattg ctgcgcctgg ttgaagtatt ccacctctat actctgaaag
     1381 tgcctaagga tgcttcgccc catctcggaa atcttggcat tggccagctc atcgagaacc
     1441 tcgacctcag tgggcagctg cagaggatca tggcgcacat tgccttccgg gaccccatcc
     1501 gaatatttgt cctccttaat cagttttcct gtaatcgtat cactgaagat attctcaccg
     1561 gtctttgtat cgacgagctt gtaggaaatc tcaatggcgg caataatctt tgccgttcca
     1621 tgcttataag ggatgtattg ataattcttc tttgtccctt cctcgctgac aaggaccttg
     1681 gcttgggaga cgcttgggtt atccatagtc tttgtcgaga aatgcagaac atcacccata
     1741 atgaagatgt ctatgccccg caccttcccg acggattggg ctgttttaat atcggcaacg
     1801 tttgccttgc cgagctgcat ctccctgatg atcgacataa ggttctcccg ttcgatgaga
     1861 cggatgtccc cgcttgcgtt tctgtgcagg taggcaagca acttcactgc ggcaatcttg
     1921 ccggcatcct tttcgttact cggagatccg aaatcaaaaa cagcaatcgc tttcttgact
     1981 ctcctgttga tgttatcctt ggcttccaac attttattaa accattccgg atgcttgcac
     2041 ttaagcgtct ccgccttttg atattggatc agggcgttgc cccacaatgc cttctcggca
     2101 tattggtcac ccctttccat catccggccg cagttgtcat ccgtgtattt cttttcagtc
     2161 gcacccggtg gctggatcgt tgctatctta atgtcttttg tattcattgt atctgaaaca
     2221 gttgtcttct tcgaaacggt cgacaaggtt gccgtagctg agggaacaaa agaaaaatcc
     2281 ccttgaagcg aagaaagttc ccaggggatc tgtttaccac ctgtattttt gccaagactg
     2341 gtcctcacct tcttgaacat atgctcaatg ctcaagccag gttccttcat atatttcaga
     2401 agcgcctttg tataagggct gttgcgcccg tcgccgtccc gcgccacttt acccggactt
     2461 gtcgaatagg aaatgaatgt ccccaccggg gcgttgctca cgatggccag accccgcacg
     2521 gcgttgcgaa aactgcgggc aaagggattg tcccggcagg cgtcgaggat gacgatgttg
     2581 aggccgttgt tggcattggc catctcatcg agaatcttcc ccgcgtccat cgtttcatac
     2641 ttgatatccg attcccggtt gatctttgcc ccgatgggaa cgagataatt cacaccatta
     2701 acctggagac catggccggc gtaaaaaaat agcccgacgc cgccacgctt caaacggtcg
     2761 ccaaattctg ttagggcagt ctccataccc tttagatttg cgtttttctt caggatgacg
     2821 gtaaacccga gcttcttcaa ctgggcatcc atagctgttg catcattgac gggattcttc
     2881 agaggacctg aactgtaggc gctattgccg atgaccaggg cggtgcgccg ctccgtcgcg
     2941 gctggcgaca aagacggggt caataaaaaa aacatgacca agacaaagaa gactcgcacg
     3001 gttttgctaa tcataattac gtccctcatt gcatattccg ccaacaaact ttcatgatca
     3061 ttgcagggcc agggggaagt ccatcccctg gagactcttc tgtgggatct gctcgtaacc
     3121 ccacttcttg taggtgattt tgggcaacgt ctcctccacg tatgtggcca ggccgttcac
     3181 cgtcaccttg ccctcccggt ccttcgctgc tcccgacatc ccttggagga gggtgtaggt
     3241 aaataccccg tgtccctcgt agccctccag ggccacctgg tccttgctgg aggccacgat
     3301 ggtggaacgt cctgtcgctc ggctcaagcg ggccaccgct gtcttctcca cgatgttgcg
     3361 ggaggccact gcttcggcga aggagccgct gttgcaggtg tcgagcagca gcagtgattt
     3421 cagcgcctgg atatgggtca ggttccgcat caggtcatcc ttggaaaccc cctgggcctt
     3481 gatcgcctcc tcgccggtga agcggaaatc gacggggagg aaataatagt tcccatcctt
     3541 ctcataggtg atcccgtgcc cggcaagaaa aaggacaaag acatcgtccc gtctcgtctt
     3601 gtcttccagc tcccggaatg ccttctccag acccgccctt gtcgcgtcat catcatagag
     3661 ggtcttcacc accaccttgc cgaagaggcg tttaccgccg ccctggatgc tccggaccag
     3721 gtcgttggcg tcagggatgg ggtacttgag gcgcaggtca ccatcccggt atttgttcac
     3781 cgccacggcg aggacgtaga ggtcgggttt ctccttgccg ccggagcgga aggtcagttt
     3841 cgcctctgcc cggttcgatt cgatggtgtt gtctttgttt ctggccatca gtccaatgac
     3901 gttttcccct gccgcgaggg tcaccctatg gcggaagctc ttacaggcaa gcggctgccc
     3961 gctgcctttt ccctggctta caaccttcag ggatcggccg tccgtgtcaa ccaccaccgg
     4021 catgccgttc aaaaagaacg tcacctcccc gatgccgccg ccctggtcgc agatctcccc
     4081 ggcgatctcc gtatcccggc tcttccgggg accggatgcc gtagtcaggc gcactgacgg
     4141 cgggagggct tccgtggtaa ggaggcggtt gacatccaca gccttggcgt actcggagat
     4201 gtccttcccc tggaacttgg ccaaaaccag gtcgggccgg tagaactggt catagagttt
     4261 gtccacattg acaaaccggg ccgcctgatc tttcccccgg ttcaaatggt agccgatcag
     4321 ctccgccccg ccttccgagg cgtcaaaaaa tccttccggc gtccagagca cccagcgctt
     4381 ccggtccttg tgggggaaga aggccaacag ttccttcccg tcggtgaggc ggtaccagcg
     4441 gatcgtcccg tcgccgaagg cggccacgcc caggcggccg tccttggaga tgttcacagc
     4501 ccaggccgcg ccgggaacca gggcttgcca tttttccttg cctttgcggt cgaaatagcg
     4561 cagataccag tccgtcccca gcaaaaagga gtcccgggac ggggcaatgg cgagactgcg
     4621 ggacatctcg tattgcctaa gcgccaacgc ccggccgttt agtttggggg atcttgaatt
     4681 tttccagtcc gttacgggga ggcccgttgt gtccggggga taaaggcccg cggcctcccc
     4741 gccagcagag agcgtccggg tggaaagatc gaacgtcgcg ggggatttac cccatagttc
     4801 gtagccgaag ctcacttttt tgccgtcgga cgagaggcga aagccttcgc aattagcccg
     4861 gtagtcagca atctgggcac tttgaaaaag gatccgctgg ccgtttttat ctagaacgcc
     4921 aaaggcgggg tctcccgcgc catagacgac acgtcccgct gccagtggga ggatggacat
     4981 gatggtgtta ggcgaacccg gcagctcccg gtgctcgccc cgactctctt ttccccaaga
     5041 aagaattgga aattggccgc ctttttgata ggttccccca gcatacagcc ggccatcggg
     5101 ggaaaaggag accttgctaa gatcgccgtt atccacgccg gaggtatccg gggaatagag
     5161 gtgggagagg tcctggccgg aaaggacatc cacccgggtg gaatcgttaa agcctacggc
     5221 cagtttctcc ccgtcggggg aaaagaaaat ggaaaatggc tgctttcccc ccggggcctt
     5281 ctgcctggcc aggagcttca ggccgccagc gccgggccgg tcgtagaggc ggatatagcc
     5341 gtccaaagag gcggcggcca gtcggccccg gcggtcaaaa acgacggagt atgaatcagt
     5401 gccgtaatct cggtcctcac ccaccggtct gccgtcggcg gcccggaaga cgcggatgcc
     5461 gttattttgt cccagcgttg ccgccagata gcgcccgtcg ggggaaaagg caagatgatt
     5521 gatcacactg gggaggccgg taatcctgcg ggtaagctca cccgtagggc ggtggaagag
     5581 gtagatgctc acaccatcat cggccagccc gccttgcggt ccggcaaatt gcgtccaccc
     5641 cccggcagcc acggtctgtc cgtccgggga aatcgccacg gcatagatct tcccttcatc
     5701 tccctcgcca ataggcggcc gcaacacaga gagcaaccgg ccggtggcca gttcccacac
     5761 ccggatactc ttgtcgtcgg aggcggtgac aagataccgg gtggcgtcat ccacgccgat
     5821 gcgcttgatg actgccgtgt gcatcgctgt ttcgattttc aggatcggtt ccttgggcgg
     5881 ttccgccgcg gtgacgggag cattaatcgg tccgagtagc atcaggcaga atatgacaat
     5941 tatccccaac cccaaaatca gtcttcgcat gaatcccacc tcttcagcaa acagggccat
     6001 agattccgtt gagcttcatc gcggcctgct gcgtgcagta ttgtataggt aatcagctta
     6061 tgtttgagat gaattgtttt cgcaaagtag tataacacat cacaagtgat tttcgtaaaa
     6121 attatccgga cactatggaa aataccattc tgccatcgga tatcctgggc ttcaggacca
     6181 accattacca atggtaattc caagtgacgc ttgggcaaac ggaaccttgc tataaagcat
     6241 ataggcacga aaaaaatccg caatcgccac accctttcaa tgctacactt gatgaggtaa
     6301 tgaactgtat actgaacatg gagcaagaga aatagaaaag gtttttgcat gctctcggca
     6361 atcgcatgat acggtagcta aggattattg gtttaatagt tttgccctta tggctgccgc
     6421 aggtctgaac atttaagcac gtagtgataa ttatcgattt gtttaatttt ttaacaggag
     6481 ttccatgtac acgtacacac acgcttactg ggacccaaaa cctttgaccc acccctccgc
     6541 tcccccgcca tctcgacacc ccggtactcc cctcgaaggt atgattaggt gacggttgac
     6601 atatctgaat tttttaccct ctggcagatc cggaagcatg ctacaggaaa cctgtcaccc
     6661 aactttgctc tgtagccgac aaggtgcaat tgtttgatcc cgggtttttc agggtgaggt
     6721 aataaccact acttcttgaa gtccacaaaa atatcatgcc ttatgaaata caagctatcg
     6781 attggtattc attacccttt attgacagaa atcaagtatg tggcaatctt gacaagcttt
     6841 ccaatccgaa atcttcgtgc ctatgccggg ggtgcttgcc caaagagcgc cacaaattgc
     6901 tttttcgatg ctccatgtgg cctaatgatc agaagattgc tgaagaagga acgccgttga
     6961 tccatatgtc gtttagtcac ccctgatctt ctatgataat atcccttgac ttgagaaaa
//
LOCUS       DAOSMF010000097         6899 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_12124_length_6899_cov_19.359293, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000097 DAOSMF010000000
VERSION     DAOSMF010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 6899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6899
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12124_length_6899_cov_19.359293"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1752
                     /locus_tag="PLT63_08635"
     CDS             <1..1752
                     /locus_tag="PLT63_08635"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HOD98828.1"
                     /translation="KAARPVWLMIVDQKVEIHAARRIWGQGIRAATAAIAEIMGPDAV
                     VAAIGPAGENLVPMSVVINDRSHSAGGVGSVLGSKNLKAIGIRGTGSIRIAGKKEDWE
                     RLIKYHLAIIGGNNQHVVPNSPQPWAEHYDPASRWNASRGRRWGAAAHRVDTGSCDPR
                     DMNRIAYRSNVAAFFLGDNAWNHTVRGNGCTSCPIRCHTILKVPSVAAKYGIPEVGQN
                     TCVGLLFGATFFKKLPDGLKGKTSLEACMVGMHLADDLGIWSNYGQLQRDFKKLYYDG
                     IIKKNLDGREFQSYSWKKYEEGDPAFLFELLPRIARKEGELATALGLGTGYLLARWGI
                     SEEEWEKDKEVAYWKLGHPKHHASEETGQCGLLINLIYNRDAQCHSHSNFLRNGLPLP
                     LQKKLAAELWGAPEAVDALGDYRPMHPAKARMAIWSLLRKELHDSLPLCNWMGPWVAS
                     PLRERGYRGDDSLESMLYTLATGDNKDRKGLDDAGMRIFLLHRALTIRGMGTKEMRTR
                     HDRIPAWVYEDQTGKGTFTKGTTCMDRDDVRTALDMFYDELGWDKATGAPTRSTYRRF
                     GLDSVGAVLAGQDLAPR"
     gene            1791..2456
                     /locus_tag="PLT63_08640"
     CDS             1791..2456
                     /locus_tag="PLT63_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98829.1"
                     /translation="MTGRGDKTGERETWADRAAALIRERSAAAELISGAELRALPAAE
                     NLAGHDATEAGDTREIGDNGEAGAALLARLLEANDDLREISGADSCSQITEGDSSLKN
                     TAAGPPYYYSSLYLTEAYARLLVHKRAGPLQLIAATVREFSGAYRRPLAVDMFMQPPF
                     LFTEEGMRTSLDRMAQTAGFEDIAATSAATGATYLYSKRFLDEGHAAMLAEWLDVGQA
                     ANP"
     gene            2732..2971
                     /locus_tag="PLT63_08645"
     CDS             2732..2971
                     /locus_tag="PLT63_08645"
                     /inference="COORDINATES: protein motif:HMM:NF015947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOD98830.1"
                     /translation="MITARVTSKGQVTIPKEIRDKLGVHTGEDVGFEEKDGMLVISKV
                     VTKSPFDKWVGKLKDLEGQRSDDLVREARGHDNSR"
     gene            2955..3437
                     /locus_tag="PLT63_08650"
     CDS             2955..3437
                     /locus_tag="PLT63_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98831.1"
                     /translation="MITAVDTNIILDVLIPGEPCGASSKELLDRHLSKGRLILCEVVF
                     AELAARFPSAKELASFLADTRMNLVYSNDKSLYMAGSRWAEYARKSAKNRFSCGKCGH
                     IFESICPQCEAILTERLHVLADFLIGAHALVQADCILSRDLGVYKTYFNDLKVIDSIR
                     "
     gene            complement(3610..4740)
                     /locus_tag="PLT63_08655"
     CDS             complement(3610..4740)
                     /locus_tag="PLT63_08655"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOD98832.1"
                     /translation="MKRNLAIDYLRSGVTILVVVHHAALAYTTFSHYDPGRYLRSTAP
                     VVDVLRWMPLDLVVGFNDMFFMPLMFLISGLFVTPSLERKGCGRFLLDRTKRLGIPFI
                     VSFMILSPLAFYPSWLLSDAVSRGDFLLDFFDGSNWTPGPAWFLWLLLAFSGVVAGAY
                     RLMPNLMKKVTLSTSSARSLVGIFLAVSLLTTVLPCLFIGPETWTRLWGPFVFQTWRF
                     FLYFAWFVLGVALGAANLELSLSSDNLRPWPLWLVLAILVFVAALLGLRPEQLANMPE
                     WVTRAISATVYSLCCTFTSLAALGLAHSFFRTAWSLADSFTANAYGVYVFHYVFVTWM
                     QFYLLTEPLPAALKFLLTLFVALTASWLLTDLLRKTVAREVL"
     gene            complement(5063..5629)
                     /locus_tag="PLT63_08660"
     CDS             complement(5063..5629)
                     /locus_tag="PLT63_08660"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOD98833.1"
                     /translation="MAKKVLVILGSPRRKGNSAILAAQITKGAKSAQAKVETIYLQEK
                     SIAPCKACFTCQKKGSKGCAIKDDMQEIYPKLLEADAWVIASPVYWFTMSAQTKIFMD
                     RCFALPAYRSDPFKGKRIAIAMTYGDEDPFASGCVNALRTFQDAFGYVEANIVGMVYG
                     SAMDAGQIKLNEKVMQEALELGKKLAAA"
     gene            complement(5809..6615)
                     /locus_tag="PLT63_08665"
     CDS             complement(5809..6615)
                     /locus_tag="PLT63_08665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOD98834.1"
                     /translation="MAADFEKGKLYQLSVIDIKPDPDQPRKYLDPQGLEELAASIKQV
                     GVLQPVLFRRGDSGYVYLVAGERRFAAAKKAGLLSIPGIYVEGSAAEIALVENLQRQD
                     LTAVEEAEALGRLVASTNYTHDQAAAVIGKSRQAVSDILSLTRLPQEIRDDCRGNAKC
                     PRAILIEIARKKQQRSMLNLYEKYKASGLTSGQLRKERQSHEAPTELETVKGAVKNLR
                     AIPGRIKKLELAALDTEEKTRLTGEIDQYVLELESRLNEIISRLESRRLS"
BASE COUNT         1553 a         1962 c         1962 g         1422 t
ORIGIN      
        1 aaggcggccc gtcccgtatg gctcatgatc gtagatcaaa aggtcgagat ccacgctgcc
       61 cgccggatct gggggcaggg aatccgcgcg gcgacggcag ccatcgccga aatcatggga
      121 ccggacgcgg tggtggccgc catcggcccg gcgggagaga acctcgtgcc catgtccgtc
      181 gtgattaatg accgttccca ctccgccggc ggcgtcggat ccgtactggg gtccaagaac
      241 ctcaaggcca tcggcattcg gggcacgggg agcatccgga tcgccggcaa aaaagaggat
      301 tgggaaaggc tcatcaagta ccatcttgcc atcatcggcg gcaataacca gcatgtcgtt
      361 cccaactcgc cccagccctg ggcggagcat tacgatccgg cgtcgcggtg gaacgcctct
      421 cggggccggc gctggggcgc ggcagcccat cgggtggaca cggggagttg cgacccccgg
      481 gacatgaacc ggattgccta ccggtccaat gtagccgcct ttttcctggg cgacaacgcc
      541 tggaatcata ccgtccgggg aaacggttgc acgagctgcc ccatccgctg tcataccatc
      601 ttgaaagtgc cgtccgtcgc cgccaaatac ggcattcccg aggtggggca aaatacctgt
      661 gtgggactcc tcttcggcgc cactttcttc aaaaaactgc cggacggcct caaaggaaag
      721 accagcctgg aggcctgtat ggtcggcatg cacctcgccg acgatctggg catctggagc
      781 aattacggtc agttgcagcg tgacttcaaa aaactttact atgacgggat catcaagaag
      841 aacttggacg gccgggagtt ccagagttac tcctggaaga aatacgagga gggagacccc
      901 gccttcctct tcgaactgct gccccggatc gcccggaaag agggtgaact ggccacggcc
      961 ctgggcctgg gtacgggcta tctgctggcg cgctggggaa tctccgagga ggagtgggaa
     1021 aaggacaagg aagtcgccta ctggaagctg ggccatccca aacaccacgc ctcggaagag
     1081 acggggcagt gcggcctcct catcaatctg atctacaacc gcgacgccca gtgccactcc
     1141 cattccaatt tcctccgcaa cggcctgccc ctgcccctcc agaagaagct ggccgccgaa
     1201 ctctggggcg cccccgaagc cgtcgatgcc ctgggggact accggcccat gcacccggcc
     1261 aaggcgcgaa tggccatatg gtccctcctc cgcaaggagc tccatgattc cctccccttg
     1321 tgcaactgga tggggccctg ggtggcctcg ccgcttaggg agcggggcta tcggggcgac
     1381 gactccctgg aatcgatgct ctatacattg gctacggggg acaacaagga tcggaaggga
     1441 ttggatgatg ccgggatgcg catcttcctc ctccaccggg ccctgacgat ccgcggcatg
     1501 gggacgaagg agatgcggac ccgccacgac aggattcccg cctgggtcta tgaggaccag
     1561 acgggcaagg gcaccttcac gaaggggacg acgtgcatgg acagagatga tgtccggacg
     1621 gccctggaca tgttctacga cgagctgggc tgggacaagg cgaccggggc gccgacccgg
     1681 agcacatatc ggcgcttcgg cctggatagc gtgggcgccg tcctggccgg ccaggacctg
     1741 gcgccgcggt gaaaactatg ccgggacgag gggacaggaa gcaggaaatt atgaccggcc
     1801 ggggcgataa aaccggggag agggaaacct gggcggaccg ggcggcggcc ctgattcggg
     1861 aaaggagcgc cgccgccgaa ctgatctccg gggcggaact gcgggcattg ccggccgcgg
     1921 aaaaccttgc tggtcacgac gctaccgagg ccggggacac cagggagatc ggagacaatg
     1981 gggaggccgg cgccgccctc ctcgcccgcc ttttggaggc aaacgatgac ctccgggaaa
     2041 tcagcggagc tgattcatgt tcacaaatca ccgaaggtga ttcatcttta aaaaacaccg
     2101 ccgccgggcc gccgtactat tattcctccc tttacctcac cgaggcttac gcccggctcc
     2161 tggtccataa acgggccggc cccctgcagc tcatcgccgc gactgtccga gaattctccg
     2221 gcgcttaccg gcggcccctg gccgtggata tgttcatgca accgcccttt ctcttcaccg
     2281 aagaagggat gcggacatct ctggaccgga tggcccaaac ggcaggattt gaggatattg
     2341 ccgccacatc cgccgccacg ggggcaacct acctctactc caaacggttc ctggatgagg
     2401 gccatgccgc catgctcgcc gaatggctcg atgtgggcca ggcggcgaat ccctgaccag
     2461 tcctcccgcc ggggccggga agtcgcgcgt gttgtgacgg tcatcctgtt ttggaatcca
     2521 ccggggacat ccggctctgg tatcctttta actgaccgca tcatttctct atttttaacg
     2581 gccgtggtca aatcgccgtt tgacatatcc cctgcattta tgtagattcc gcgatcctcc
     2641 ctttgacacg gccttcataa attcttgaca aggaacggta atacccttac aatacaagta
     2701 aggaaaaata ctccttactg agaggtgagc gatgataacc gcaagggtta catccaaggg
     2761 acaagttact attcctaagg aaataagaga taaactgggc gtccatacgg gtgaggatgt
     2821 gggttttgaa gaaaaagacg gtatgctggt tatcagtaag gtagtcacaa aatccccgtt
     2881 cgacaaatgg gtgggcaagc tgaaagacct ggaagggcag cgaagcgacg atctggtgcg
     2941 ggaggcccgt gggcatgata acagccgttg acacaaacat catcctcgat gtcctgattc
     3001 cgggtgagcc ttgcggagcg tcctccaagg aacttttgga ccgacatcta tcaaagggta
     3061 ggttgatcct ctgcgaggtg gtttttgcag aactggccgc ccggtttcct tccgcaaagg
     3121 aacttgcgtc gtttctcgcc gacaccagga tgaacctcgt ctattccaat gataaatccc
     3181 tctatatggc cggttcaaga tgggcggaat acgcaaggaa aagtgcgaag aacaggtttt
     3241 catgcggtaa atgcggccac atctttgagt cgatctgtcc ccagtgcgag gcaatcctga
     3301 cggagcgact ccatgtcctg gccgattttc tgatcggcgc ccatgccctt gtccaagccg
     3361 actgtattct ttcacgggat cttggcgtgt ataaaaccta cttcaacgac ctgaaggtaa
     3421 tcgactcgat aaggtaaagg ttaaatggaa aggtcatcca agcaccctgc acctttggtg
     3481 ggcaaggcgg ccgggcgggg cggcaagagc ggtgatcttt gcccagatgg tggacgaccc
     3541 ttctgcacat cctgatctgt ttcccacgga aaagcacagg aaaaagaacg gaaacgtctc
     3601 ttcaagtcat caaaggactt ctctcgccac tgtcttgcgc aataaatctg tcaggagcca
     3661 gctcgcggtc agggcgacaa agagggtgag caggaattta agcgctgccg gcaagggctc
     3721 cgtgagcagg tagaattgca tccaagtcac aaaaacgtaa tgaaagacat agacgccgta
     3781 tgcgttcgct gtgaagctgt ccgctagcga ccaggcagtt cgaaagaaag aatgggcaag
     3841 acccagcgcg gcaagactgg taaaggtgca acagagggaa tagactgtcg ctgagattgc
     3901 tcttgttacc cattccggca tgttcgcaag ctgctccggc ctcaatccca gcagcgccgc
     3961 cacaaaaact aagatcgcca gaacaagcca gagcggccat ggtcttagat tgtcggatga
     4021 taatgacagt tccaggtttg cggcaccgag cgcaaccccc aacacaaacc aggcaaaata
     4081 caggaagaac cgccaggtct gaaaaacgaa cggaccccac aaacgggtcc atgtctcggg
     4141 cccaatgaac aggcacggca gaacggttgt caacaggctc acggcaagga atatgccgac
     4201 aagacttcga gccgatgagg tggagagggt cacttttttc atgagatttg gcatcaatcg
     4261 gtaggcgccg gcgacaacgc cggagaaagc cagcaagagc cagaggaacc aagccggccc
     4321 cggcgtccag ttgctgccgt cgaagaaatc aagcaggaag tccccccggc tgaccgcgtc
     4381 gcttaagagc caggatgggt agaacgccag ggggctgagg atcatgaagg agacaatgaa
     4441 gggaatacca agacgtttcg tgcggtccag aagaaaacgg ccgcatccct tccgttcgag
     4501 ggatggtgtc acgaatagcc cggagatgag gaacataagc ggcatgaaga acatatcgtt
     4561 gaatcccaca acaaggtcga gcggcatcca gcggagcaca tcgacgactg gcgcggtgga
     4621 tctcaaatac cgccccgggt cgtaatgact gaaagtggtg tacgccaagg cagcgtgatg
     4681 tacaactacc aggatggtta ccccgctcct taggtagtcg atcgccaggt ttcgcttcaa
     4741 atggctcctc caagagcgaa aagcgatagc ttagctccgc cgttgccgac gcgcgctgcg
     4801 cgacagatca cttgccgtgt agaattcgaa cggttcaggc cgtgcgctga ttctttctaa
     4861 ctgttcaaaa attttctctc tcatcgtcga tatggcacgg aacaatcaac ataccaacct
     4921 ggttttttcc gatttacgga ggcaatatta ctctttgccg catggaatac tgcatcataa
     4981 gcttgccgca ttcatctctg gctggtatgc tatttgtgct tttattctct gaagtcatcc
     5041 atgtctggtt atcccacccc tttcaagctg ctgccagttt ctttcccagt tcaagggctt
     5101 cttgcatgac tttttcattt aacttgatct gaccggcatc catggcgctt ccatagacca
     5161 taccgacaat attggcttct acatatccaa aggcatcctg aaatgtcctc agggcattga
     5221 cgcatcccga agcaaaggga tcctcatcac cataggtcat ggcaatagcg atacgtttgc
     5281 ccttgaaggg atcgctccgg taagccggca acgcaaaaca tctatccatg aatattttgg
     5341 tctgagccga catggtaaac caataaacag gactggcaat cacccaggca tcagcttcaa
     5401 gcagtttcgg atagatttcc tgcatatcat ccttaatggc gcaacccttg ctgcccttct
     5461 tctgacaagt gaaacaggct ttgcagggag caatgctctt ttcctgcaaa tagattgtct
     5521 cgacttttgc ctgggcggat tttgcccctt tggttatctg tgccgccaat atggcgctgt
     5581 tgcctttcct ccttggactc cccagtatta ccagaacctt ttttgccatg ataatgcctc
     5641 ctgatttata ttgatcttgc cgctacatac ttttatcccg ccccgccgga aaaactccgc
     5701 cggtgcaccg gcagaggata actatacgat ataatatgtt tattgacgat gttgcgcccc
     5761 gtttttctct tcctatcccg cttgagagcc cccgtttccg ccccgccttc aggacagtct
     5821 ccgggactcc aaccgggaga tgatctcgtt gaggcgggac tccagttcca aaacgtattg
     5881 atcgatttcg ccggtaaggc gggtcttctc ctccgtgtcc agggcggcga gctccagctt
     5941 tttgatccgt cccggaatgg cccgcaggtt cttcacggcg cctttgacgg tctccagttc
     6001 cgtcggcgcc tcgtgggatt ggcgctcttt gcggagctgg ccgctggtca ggccgctggc
     6061 cttgtatttc tcatagaggt tgagcatgga gcgttgctgc ttcttccggg cgatctcgat
     6121 gaggatcgcc cgggggcatt tggcattgcc ccggcaatcg tcgcgaatct cctggggaag
     6181 gcgggtcagg gacaggatat ccgacaccgc ctggcggctc ttgccgatga ccgccgcggc
     6241 ctgatcatgg gtgtagttcg tggacgccac gagacggccc agggcctccg cctcctcgac
     6301 ggccgtcagg tcctggcgtt ggaggttctc caccagggcg atctcggcgg cggaaccttc
     6361 cacgtagatg cccgggatgg agagaagccc ggccttcttg gccgcggcga aacgccgctc
     6421 ccccgccacg agatagacat agcccgagtc gccccggcgg aagaggacgg gctggaggac
     6481 gccgacctgt tttatggacg cggccagttc ctccaggccc tggggatcca ggtatttccg
     6541 gggctgatcg gggtccggtt tgatgtcgat tacggacaat tggtagagtt ttcccttctc
     6601 aaagtcggcg gccataaata cctcccgttt gctttattag gaaaggcttt gaataattga
     6661 aagcttttcc aattataagc atagggataa caaagcgaga tgtcaacaag atatttatta
     6721 tcttcaataa ttcccctttt cgactggcgg ggtaaggcca ggccggcatc tgcccaaaca
     6781 tctcaaaggc ctgatagggc aatttcagct ccgggtaggc cgcaaacgaa ttctccacgt
     6841 aatagttgac ggcatcgtaa aaagtccgga tgcttcgaaa gcgcttcatc cctcgtcac
//
LOCUS       DAOSMF010000098         6871 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_12186_length_6871_cov_21.396567, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000098 DAOSMF010000000
VERSION     DAOSMF010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 6871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6871
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12186_length_6871_cov_21.396567"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..>1587
                     /gene="guaA"
                     /locus_tag="PLT63_08670"
     CDS             83..>1587
                     /gene="guaA"
                     /locus_tag="PLT63_08670"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF000848.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOD98835.1"
                     /translation="MILIIDFGSQYNQLIARRVREHHVYCQIEPPDIPLAVIRDMHPE
                     GIILSGGPASIYEKNSPRVDTGIFTLGIPVLGICYGLQFMVHALGGEVIAAAKREYGF
                     AELDVLHPGDVLAGVEKATRCWMSHGDAIGGLPAGFRATAATPNTPVAAAEDARRRFY
                     GLQFHPEVVHTPEGRKILGNFLFVVCGCKKDWTMKSFARDAVAEIREQVGNGRVILGL
                     SGGVDSSVAAVLLHRAVGPRLTCVFVDNGVLRHGEAKKVRELFRNHFHVNLRFVRAGR
                     IFLDRLKGVEAPEKKRKIIGRTFIEIFDGEAKKIEGADFLAQGTLYPDIIESRSAFGG
                     PSAVIKSHHNVGGLPKRMKLKLVEPLRHLFKDEVRLLGKELGLPEEMVWRQPFPGPGL
                     AIRIIGAVTEKRLKILKQADVILLEEIRAAGLYRKLWQSFAVLLPVKSVGIMGDQRTY
                     ENILAIRAVTSDDAMTADWARLPQELLARISNRIINEVRGVNRVVYDISSKP"
     assembly_gap    1588..1687
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1688..>3025
                     /gene="carB"
                     /locus_tag="PLT63_08675"
     CDS             <1688..>3025
                     /gene="carB"
                     /locus_tag="PLT63_08675"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOD98836.1"
                     /translation="EFDYSGTQACKALKEEGYEVVLINSNPATIMTDPETADRTYVEP
                     ITPEAVAKIIARERPDALLPTLGGQTGLNTAVAVAENGTLAAYGVEMIGASLEVIHKA
                     EDREKFRHAMEKIGLRVPRSGFARNMADVLRSAGEIGFPVIIRPSFTLGGTGSGVAYN
                     REELVEIAKAGLDASMIHEIMLEESALGWKEYELEVMRDRADNVVIICSIENFDPMGV
                     HTGESITVAPAQTLTDREYQHMRDAAIAIMREIGVETGGSNVQFAVHPDTGEMIVIEM
                     NPRVSRSSALASKATGFPIAKIAAKLAVGYTLDEIPNDITRETMASFEPTIDYVVTKI
                     PRWTFEKFPETEDFLTTSMKSVGETMAIGRTFPESLQKAIRSLEIGRFGLLDKLPAEA
                     ARNPREYFAPKLRTPNSLRLFHAAAALAAGMTGVEIHALTKIDPWFLYYLEEIV"
     assembly_gap    3026..3125
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3126..5241
                     /gene="carB"
                     /locus_tag="PLT63_08680"
     CDS             <3126..5241
                     /gene="carB"
                     /locus_tag="PLT63_08680"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOD98837.1"
                     /translation="HALTKIDPWFLYYLEEIVAAEKALAAAREYIRVHLPLPGSSRCL
                     SPAMDGAAAGDNACNGAPLAGMPVSEVMPTVTDEQRHYQGAAGIFPAPDAPAGTEEGA
                     AAPDPPALSPVELLRAAKEMGFSDRRLAEIWNTDEETIRRRRREKGILPVYKLVDTCA
                     AEFAAYTPYYYSTYETEDEANPSDRPKVVILGGGPNRIGQGIEFDYCCVHASFALREE
                     GIESIMVNSNPETVSTDYDTSDKLFFEPLTREDVLHIIDRENPRGVIVQFGGQTPLNL
                     AKGLAAAGARILGTSPDAIDRAEDRKRFQEIVNLLELKQPANDTAMDTEAAVRAAARI
                     GYPVVVRPSYVLGGRSMEIVYDEQTLRAFMAKAVLVSPGHPILIDQFLEDAVEVDVDA
                     ISDGADTVIGGIMEHIEEAGIHSGDSACILPPQTLAPEMIARIETQTKQIAAALGVVG
                     LMNIQYAIKDGELYLLEVNPRASRTVPFVSKAIGVPLAKLATKVMLGKTLKELGFTER
                     RHPRHIAVKEAVFPFSRFPGVDVLLGPEMKSTGEVMGIDSSFGMAYAKSQLAAGWRLP
                     LSGTVYISVHNRHKEKTVAIARAFQEMGFRIVGTRGTAAFLGDHGIPITPINKLTEGR
                     PHVLDAIKNGSIQLMINTSVGRKSSYDGYNIRRGALIYNIPYTTTLAGAKALSEAIAA
                     LKSRDWEVTPLQDYYQAGRKLS"
     assembly_gap    5591..5690
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5691..>6871
                     /gene="purF"
                     /locus_tag="PLT63_08685"
     CDS             <5691..>6871
                     /gene="purF"
                     /locus_tag="PLT63_08685"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOD98838.1"
                     /translation="GRPREECGVFAVFGHPEAARMTFFGLFALQHRGQESAGIVSADG
                     CDIWHHKGMGLASEVFKEDNLSRLKGHLAIGHVRYSTTGSSVISNAQPFVAHHGQEYY
                     AIGHNGNLTNTRQLRAALEERGSIFQSTMDTEVIVHLMAARLKNGFDAALSAALGEVE
                     GAYSLVMLTRHRIIAARDPRGFRPLCLGLINGGWVVASETCAFDLVGAAYVRDIAPGE
                     IVIIDETGVKSLTPFPEQKYAHCIFELIYFARPDSQIFGRNVYLCRKRLGHRLAQEYR
                     PDVDLVMPFPDSGNYAALGYAEESGLPFEMGMIRNHYVGRTFIQPTQPVRDFGVRVKL
                     NPVRPLLEGKRILIVEDSIIRGTTSRNRVRNLRNNGAREIHMVISCPPTRHPCPYGID
                     FS"
BASE COUNT         1245 a         2278 c         1969 g         1079 t
ORIGIN      
        1 catgcccgtt ccgggcacaa cgaaagatga aaatatccgg ctacttgtgg acattttcat
       61 ataaaactaa aggagttcca gcttgatcct catcatcgac ttcggttccc agtacaacca
      121 actcatcgcc cggcgggtcc gggaacacca cgtctattgc cagatcgaac cgccggacat
      181 ccccctggcc gtcatccggg atatgcatcc cgaggggatc atcctctccg gggggccggc
      241 gagcatctac gagaagaaca gcccccgggt ggacaccggc atcttcaccc tgggcatccc
      301 cgtcctcggg atctgctacg gcctccagtt catggtccac gccctggggg gcgaggtcat
      361 cgccgccgcc aagcgggaat acggcttcgc cgaactcgat gtcctgcacc ccggcgacgt
      421 cctggccggg gtggaaaagg ccacccggtg ctggatgagc cacggcgacg ccatcggcgg
      481 gctccccgcc ggtttccgcg ccaccgccgc cacgcccaac acccccgtcg ccgccgccga
      541 ggacgcgcgg cggcgtttct acggcctcca gttccacccg gaggtggtcc acaccccgga
      601 ggggcggaag atcctcggca acttcctctt tgtcgtctgt ggctgcaaaa aggactggac
      661 gatgaaatcc ttcgcccggg acgccgtcgc ggagatccgg gagcaggtgg gcaacggcag
      721 ggtcatcctc ggcctgagcg gcggcgtgga ttcctccgtg gccgccgtcc tcctccaccg
      781 cgccgtcggc ccccggttga cctgcgtctt cgtggacaac ggcgttttgc gccacgggga
      841 ggcgaagaag gtccgggagc tgttccgcaa tcatttccat gtgaacctcc gttttgtccg
      901 ggcggggagg atcttcctcg accgcctcaa gggggtggag gcgccggaaa agaagcgcaa
      961 gatcatcggg cggaccttta tcgagatctt cgacggcgag gcgaaaaaga tcgaaggggc
     1021 ggattttctc gcccagggga ccctctatcc cgacatcatc gaatcccgtt ccgccttcgg
     1081 cggccccagc gccgtcatca agtcgcacca caacgtcggc ggcctgccga agcggatgaa
     1141 gctgaaactc gtggagcccc tgcggcacct cttcaaggac gaggtccggc tcctgggaaa
     1201 ggaactgggg cttcccgaag agatggtgtg gcgccagccc ttccccggcc cgggactcgc
     1261 catccgcatc atcggcgccg tcacggaaaa gcgcttgaag attctcaagc aggcggatgt
     1321 aatcctcctg gaggagatcc gggccgccgg cctctaccgg aaactctggc agtccttcgc
     1381 cgtcctcctg cccgtcaaga gcgtggggat catgggggac cagcggacct atgaaaacat
     1441 cctggcgatc cgtgccgtga ccagcgacga cgccatgacg gcggactggg cgcgcctccc
     1501 ccaggaactc ctggcgcgga tctccaaccg gatcatcaac gaggtccggg gggtcaaccg
     1561 cgtcgtctat gacatcagct ccaagccnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnngaa ttcgactatt cggggaccca ggcctgcaag gccctgaagg aagagggcta
     1741 cgaggtcgtc ctgatcaaca gcaacccggc gacgatcatg acggacccgg aaacggcgga
     1801 ccggacctac gtggaaccca tcaccccgga ggcggtggcc aagatcatcg cccgggagcg
     1861 gcccgacgcc ttgctcccca ccctgggggg gcagacgggc ctgaacaccg ccgtggccgt
     1921 ggcggaaaac ggcaccctgg ccgcgtacgg tgtggagatg atcggcgcct ccctggaggt
     1981 gatccacaag gcggaggatc gggagaagtt ccgtcacgcc atggaaaaga tcggcctccg
     2041 agtcccccgg agcggcttcg cccggaacat ggccgacgta ctgcgcagcg ccggcgagat
     2101 cggcttcccc gtcatcatcc gtccttcctt caccctgggc ggcacgggca gcggcgtggc
     2161 ctacaaccgc gaggagctcg tggagatcgc caaggcgggc ctcgatgcca gcatgatcca
     2221 cgagatcatg ctggaggagt cggccttggg ttggaaggaa tacgagctgg aggtcatgcg
     2281 ggaccgggcg gacaacgtgg tcatcatctg ctccatcgag aatttcgatc ccatgggcgt
     2341 ccataccggg gagtccatca ccgtcgcccc cgcccagacc ctgacggacc gggaatatca
     2401 gcacatgcgc gacgccgcca tcgccatcat gcgggagatc ggcgtggaga ccggcggctc
     2461 caatgtccag ttcgccgtcc accccgacac gggggagatg atcgtcatcg agatgaaccc
     2521 ccgggtgtcc cggtcctccg ccctggcctc caaggccacc ggcttcccca tcgccaagat
     2581 cgccgccaag ctggccgtgg gttacaccct ggacgagatc cccaacgaca tcacccggga
     2641 gaccatggcc tccttcgagc cgaccatcga ctacgtggtc accaagatcc cccgctggac
     2701 cttcgagaag tttccggaaa cggaggattt cctcaccacc tctatgaagt ccgtggggga
     2761 gacgatggcc attggccgga ccttcccgga atccctccag aaggcgatcc gctccctgga
     2821 gatcggccgt ttcggcctcc tggacaaact gcccgcggag gccgcgagga acccccggga
     2881 atacttcgcc ccgaagctgc ggacccccaa ctccctgcgc ctcttccacg cggcggccgc
     2941 cctggccgcc ggtatgaccg gcgtggagat ccacgccctg acgaagatcg acccctggtt
     3001 tctttattat ctcgaggaaa tcgtcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnccacg ccctgacgaa gatcgacccc tggtttcttt attatctcga ggaaatcgtc
     3181 gcggcggaaa aggctcttgc cgccgcccgg gagtatatcc gggtacacct cccccttccc
     3241 ggttcatccc gctgcctctc cccggcgatg gacggcgccg ccgccggcga taatgcctgt
     3301 aatggcgctc cgttggcggg aatgcccgtc agcgaagtca tgccgacagt tacggatgaa
     3361 caacggcatt atcaaggcgc ggcagggatt tttcctgccc cggacgcacc ggcagggacg
     3421 gaagaagggg ccgccgcccc tgatcccccc gccttgtccc ccgtcgagct cctccgggcc
     3481 gccaaggaaa tgggtttctc cgaccgccgc ctcgcggaaa tctggaatac cgacgaggaa
     3541 accatccgcc ggcgccgccg ggaaaagggc atcctaccgg tctataaact cgtcgatacc
     3601 tgcgccgcgg aattcgccgc ctatacgccc tactactatt ccacctacga aaccgaggat
     3661 gaggccaacc cctccgaccg ccccaaggtg gtcatcctcg gcggcggccc caaccgcatc
     3721 ggccaaggca tcgagttcga ctactgctgc gtccacgcct ccttcgccct ccgggaggag
     3781 ggcatcgaga gcatcatggt caactcgaac cccgagacgg taagcacgga ttacgacacc
     3841 tccgacaagc tcttcttcga gcccctgacc cgggaggacg tcctccatat catcgaccgg
     3901 gaaaatcccc ggggggtcat cgtccagttc ggcggccaga cccccctgaa cctggcgaag
     3961 gggctggcgg cggccggggc gcggatcctg gggacctccc ccgacgccat cgaccgggcg
     4021 gaagaccgga agcgcttcca ggagatcgtc aacctcctgg agctcaagca gcccgccaac
     4081 gatacggcca tggacaccga agcggccgtc cgggcggcgg cgcggatcgg ctatcccgtc
     4141 gtggtccgcc cgtcctacgt cctgggcggc cggtccatgg aaatcgtcta tgacgagcag
     4201 accctccggg cctttatggc caaggccgtc ctggtttccc ccggccaccc catcctcatc
     4261 gaccagttcc tggaggacgc cgtggaggtg gacgtggacg ccatcagcga cggcgccgac
     4321 accgtgatcg gcggcatcat ggagcacatc gaggaggcgg ggatccactc cggcgacagc
     4381 gcctgcatcc tcccgcccca gaccctggcc ccggaaatga tcgcccgcat cgagacgcag
     4441 acgaaacaga tcgccgcggc gctgggcgtc gtgggcctga tgaacatcca gtacgccatc
     4501 aaggacgggg agctctacct cctggaggtg aacccccggg cctcccggac cgtccccttc
     4561 gtgagcaagg ccatcggcgt ccccctggcc aagctggcga cgaaggtgat gctgggcaag
     4621 accctgaagg aactgggctt tacggagcgg cgccaccccc ggcacatcgc cgtcaaggag
     4681 gcggtattcc ccttcagccg cttccccggc gtggatgtac tcctgggtcc ggagatgaaa
     4741 tccaccggcg aggtcatggg catcgattcc tccttcggca tggcctacgc caagtcccaa
     4801 ctggccgccg gctggcgcct cccgctctcc ggcaccgtct atatcagcgt ccacaaccgg
     4861 cacaaggaga aaaccgtggc catcgcccgg gccttccagg agatgggctt ccggatcgtc
     4921 ggcacccggg ggaccgccgc cttcctggga gatcacggta tccccatcac ccccatcaac
     4981 aaactgacgg agggccgtcc ccacgtcctg gacgccatca agaacgggtc catccagttg
     5041 atgatcaaca ccagcgtggg ccggaagtct tcctacgacg gctacaacat ccgccggggg
     5101 gcgctgatct acaacatccc ctacaccacc accctggcgg gtgccaaggc cctgagcgag
     5161 gccatcgccg ccctgaagag ccgggactgg gaggtaacgc ccctgcagga ttactatcaa
     5221 gcgggaagga aactctcatg acggcttgcc ttcacaaagg gggatgaaaa atagacccat
     5281 ccccacctta atcctcccct tgaaggggag gaaattaatt ggtgaaggaa gtgaactaca
     5341 taggaacgca ttttcaggtg aaacattcat gtgcgtgccg tcacatattc atttaaatgt
     5401 gcaaccgggg gaaaggtcat tgtttctccc cccgcaaggg ggaggtcagg taggggatgg
     5461 ggccaatttt accgcgtcgt cagcttttca catgagcaaa gaaccgtgac ggaaataatt
     5521 gattatttcg acaccggccg cccccgggag gagtgcggcg tcttcgccgt cttcggccat
     5581 cccgaggcgg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ggccgccccc
     5701 gggaggagtg cggcgtcttc gccgtcttcg gccatcccga ggcggcccgg atgacctttt
     5761 tcggcctctt cgccctccag caccggggcc aggagagcgc cggcatcgtc tccgccgacg
     5821 ggtgcgatat ctggcatcac aaggggatgg ggctggcctc cgaggtcttc aaggaagaca
     5881 acctctcccg gctcaagggc catctggcga ttggccatgt ccggtactcc accaccggtt
     5941 cgtcggtcat ctccaacgcc cagcccttcg tggcccacca cggccaggaa tactacgcca
     6001 tcggccacaa cggcaatctc accaacaccc ggcagctccg cgccgccctc gaggagcggg
     6061 gctcgatctt ccaatccact atggacacgg aggtcatcgt ccatctcatg gccgcccggc
     6121 tgaaaaacgg ctttgatgcg gccctgagcg cggccctggg cgaagtggag ggggcctaca
     6181 gcctcgtcat gctcacccgc caccggatca tcgccgcccg ggacccccgg ggattccgcc
     6241 ccctctgcct gggcctaatc aacggcggct gggtggtggc ctcggagacc tgcgccttcg
     6301 acctcgtggg ggcggcctac gtccgggaca tcgccccggg tgaaatcgtc atcatcgacg
     6361 agaccggcgt aaagagcctc acccccttcc cggaacaaaa gtacgcccac tgcatctttg
     6421 agctgatcta tttcgcccgg ccggacagcc agatcttcgg ccggaacgtc tatctgtgcc
     6481 ggaagcgcct cgggcaccgc ctcgctcaag agtaccgccc cgacgtcgat ctcgtcatgc
     6541 ccttccccga ttccggcaac tacgccgccc tgggctatgc cgaggagagc ggcctgccct
     6601 ttgaaatggg gatgatcaga aaccactacg tgggccggac cttcatccag cccacccagc
     6661 ccgtccggga cttcggcgtc cgggtcaagc tcaacccggt gcgccccctg ctggaaggca
     6721 agcgcatcct catcgtggag gattccatca tccgcggcac caccagccgg aaccgggtcc
     6781 ggaacctccg caacaacggg gcgcgggaga tccacatggt gatcagttgc ccgcctaccc
     6841 gccacccctg cccctacggt atcgactttt c
//
LOCUS       DAOSMF010000099         6734 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_12492_length_6734_cov_20.402455, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000099 DAOSMF010000000
VERSION     DAOSMF010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 6734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6734
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12492_length_6734_cov_20.402455"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1280
                     /locus_tag="PLT63_08690"
     CDS             <1..1280
                     /locus_tag="PLT63_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HOD98839.1"
                     /translation="LALSIIISCGTIAGALIPEFTKVFTSTSSRHCDECVNASRQGGA
                     SLNILSGLVAGNFSAFWEGLTILFLMFIAYFVSQAPSVMEIMPAAFKFAAPVFAFGLV
                     AFGFLGMGPVTIAVDSFGPVSDNAQSIYELSMIESRPDAREVVKQHYGIDPDFEAAKH
                     CLESADGAGNTFKATAKPVLIGTAVVGATTMVFGIIILLEKMFGHVVAKLSLVQPEVI
                     LGLLMGGCVIYWFTGASIQAVVTGSYRAVVYIKENIDLDKTEASIEDSKEVVKICTIY
                     AQKGMINIFIVIFFMALGLAFVDPYFFIGYLIGIAFFGLFQAIFMANAGGCWDNAKKI
                     VEVDLKQKNTPLHEASVVGDTVGDPFKDTSSVSMNPIIKFTTLFGLLATEIAVTMTNQ
                     GMKVGLAVAFFVIALIFVYRSFYGMRISAEKLG"
     gene            1504..2598
                     /gene="ychF"
                     /locus_tag="PLT63_08695"
     CDS             1504..2598
                     /gene="ychF"
                     /locus_tag="PLT63_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003026825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOD98840.1"
                     /translation="MGFRCGIIGLPNVGKSTIFNALTATGAEVANYPFCTIDPNIGVV
                     SVPDPRLDFIAEILHPLKKTYTTMEFLDIAGLVKGASRGEGLGNKFLGHIREVDAIVH
                     IVRCFDNPDVVHVDGLVDPVRDIEIVNTELLLADLETIDKRLLSVEKRVKTGDREAGQ
                     TLEFLRMIKDTLGRGIPCRNLVPTEEHTEVRKDLHLLTDKKVLYTANVSEAVLKGAQG
                     YVSAMEEVAAREGALVITICGDMEAEIVELPPEERAVFLEDLGLRESGLEKLIRAGYD
                     LLGLITFYTTVGTELRAWTIPRGTRANQAAGKIHSDMERGFIRAEILHFEDFKAVGNI
                     AVAREKGLVRSEGRDYQINDGDIVVFRFNV"
     gene            complement(2747..2968)
                     /locus_tag="PLT63_08700"
     CDS             complement(2747..2968)
                     /locus_tag="PLT63_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98841.1"
                     /translation="MSVEEKNHSQQDSGSEEDSEYFRLDRQIVHDFFLSVDSGRKAEG
                     LTEREVSYKKNEVKEEKREDFFLPDSSCD"
     gene            complement(3279..5399)
                     /locus_tag="PLT63_08705"
     CDS             complement(3279..5399)
                     /locus_tag="PLT63_08705"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HOD98842.1"
                     /translation="MVELSDGVAGPVLDKDRLFKSIFRISNLLTIQANQDEIMAKILD
                     ELVDAVGFERGIIRLCDETQQYLVTKVVKNYLEEETIRAFSTPLNINEHDCLATKVAR
                     TGDMIMIRNAATDPRITPMDRMLTNIVGRGSIVCTPLKIGDYVIGTLAAWRQEEVDFF
                     AEEINLFLTFSNQVSVIIHNARLLETNAEKIRHLMILQEAVSEMNQSYSLDNRILEIV
                     FISALKIAYADRVMGYVWDIEKDRCLINDGERVVVKSKQECDTTMSASLIREAIEKNT
                     TVVTTAMEGKGIQPLFPDFPMEIAIPFHIRDKFVGAMLLAKKKGSFSSDQVNILDVLI
                     KNAATAYDNAIMHSLLSLEAETLKTEVEKLKEREDHLMGFHDILGKSRKMIDIFHVIA
                     DVAGHDTNILIQGESGTGKELIARAIHRHSHRNLKPFVDINCAAIPATLLESELFGYE
                     AGAFTDARKRKIGLLEYCFGGTMLLDEIGEMPIQLQAKFLRMLEDGHIRRLGGNENIP
                     IDVRFVFSTNRDLSEMVAAGTFREDLFYRISVVPVIIPPLRERSDDIIMLARYFVQEF
                     NKKFSKRVKGFNREAEAILQAYSWPGNVRELKNIVERIMILKGVGNVISPDNMPTELN
                     MLANKDVDIKIEPFLQQLPLTGIDYDTVTEKILKDVKGKILENALQKSGGNKTEAARQ
                     LGISRYKLIREQKKYQEEKQEAPS"
     gene            complement(5459..5971)
                     /locus_tag="PLT63_08710"
     CDS             complement(5459..5971)
                     /locus_tag="PLT63_08710"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOD98843.1"
                     /translation="MIQEAAMLQQTEIFRGLSPEQIGEVLGIARTVTFPAGAVIMREG
                     EVGGTMYIMMSGTVEVVKSLVIGELEDEQDAGRNKVFTRLDAKDHAVFGEIALLEAQK
                     RTATIKAVTNCDLYEFSKDDFLQLAEDKYDLGFRILLNMARIVSTRLRKADEETVKLT
                     TALSIILKEL"
     gene            complement(6002..6580)
                     /locus_tag="PLT63_08715"
     CDS             complement(6002..6580)
                     /locus_tag="PLT63_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98844.1"
                     /translation="MADQSGGHSQERSDGRTALAALTVKSLAPHVKTIAEILDGDNRP
                     HLRRANVDEIVVRGEHVGALLASAINSPGLPRVISSLVSLGDKNKLWRTQIPAGFINR
                     PFQELAAHNREREQAILIGIMKERKAIKLEDILSGNTSVIDNFIRERIREANKDFSTE
                     KDALKININPEGDYMIAADDLAVVLSRTMPSS"
BASE COUNT         1545 a         1802 c         1650 g         1737 t
ORIGIN      
        1 ggctggccct ttccatcatc atcagttgcg ggaccatcgc cggcgccctg atcccggaat
       61 tcacaaaggt cttcacgagc accagttccc gtcactgcga cgagtgcgtt aatgcctccc
      121 gccagggcgg tgcttcgctg aacatccttt ccggattggt cgccggtaac ttctccgcct
      181 tctgggaagg tctgaccatc ctgttcctca tgtttatcgc ctattttgtt tcccaggccc
      241 cgtcggtcat ggagatcatg ccggcggcct tcaagttcgc cgccccggtc tttgccttcg
      301 gtctcgtggc cttcggattt ctcggcatgg gtcccgtgac catcgcggta gatagcttcg
      361 gccccgtatc ggacaatgcc cagtccattt atgaactctc catgatcgaa tcaaggccgg
      421 acgcccggga agtagtgaaa caacattacg gcatcgaccc tgatttcgag gcggccaagc
      481 actgtctgga atcagcggac ggcgcgggca acaccttcaa ggccacggcc aagccggtcc
      541 ttatcggcac cgccgtcgtc ggcgccacaa ccatggtctt cggcatcatc atcctcctgg
      601 agaagatgtt cggccatgtg gtagccaagc ttagcctcgt ccagccggag gtcatcctcg
      661 gcctcctcat gggcggctgc gtgatctact ggttcaccgg cgcctcgatc caggctgttg
      721 tcaccggctc ctaccgtgcg gtagtttata taaaggaaaa catcgacctc gacaagacgg
      781 aagcctctat cgaagacagc aaggaagtgg tgaagatctg tacgatttat gcccagaagg
      841 ggatgatcaa catcttcatc gtcatcttct tcatggccct ggggctggcc ttcgtcgatc
      901 cctacttctt tatcggctac ctcatcggca tagccttctt cggccttttc caggccatct
      961 tcatggccaa cgccggcggt tgctgggata acgccaagaa gatcgttgaa gtcgatctga
     1021 agcagaagaa caccccgcta cacgaggcct ccgtcgtggg cgacaccgtc ggcgacccct
     1081 tcaaggacac ctcttcggta tccatgaacc cgatcatcaa gttcacgacc ctgttcggcc
     1141 tcctggccac ggaaatcgcc gtgacgatga ccaatcaggg tatgaaggtg ggtttggccg
     1201 tcgcattctt cgttatcgcc ctgatcttcg tgtatcgttc cttctacggt atgcggatct
     1261 ccgcagagaa gctgggctaa catcgaatca atcctgaccc aatcaggata cgcaacatga
     1321 aaagcgcctc ccgaaacggg aggcgctttt ttatgggctt gacaaaactc gggaaaccca
     1381 ttagtcccaa aaacggttta tcttccgcac ctccaagagt ctctccggaa gcgcttcgtg
     1441 cgtcctgtgt gcgctgcgat gattttcccc ggtgggacga tcataacaca aaggagtcta
     1501 accatgggct ttcgctgtgg aattatcggc ctccccaatg taggcaagtc aacgatcttc
     1561 aacgccctta ccgccaccgg ggcggaagtg gccaattatc ccttctgtac catcgatccc
     1621 aacatcgggg tggtgtccgt tcccgatcca cggcttgatt ttattgctga aatcctccat
     1681 cctctgaaaa agacgtatac aaccatggaa tttctggata ttgccggcct ggtaaaaggg
     1741 gccagcagag gagagggatt gggcaacaag tttctcggtc atatccggga ggtggatgcc
     1801 atcgtacata ttgtccgctg tttcgataac cccgatgtgg ttcatgtcga tggtttggtc
     1861 gatccggtac gggacatcga gatcgtcaac accgaattgc tgcttgccga tctggaaaca
     1921 atcgacaagc gcctcctgtc ggttgagaaa agagtcaaga caggcgaccg ggaggcaggt
     1981 caaaccctgg agttcctgcg catgatcaaa gataccctgg gacgtggcat accctgccga
     2041 aatcttgtcc ccacggagga gcatacggaa gtgcgtaaag atttgcacct attgacggat
     2101 aagaaggtac tttatacggc caacgtcagc gaagccgtgt tgaaaggggc gcagggatat
     2161 gtcagcgcca tggaggaggt tgccgctcgt gaaggcgcct tggtcataac aatctgcggg
     2221 gatatggaag cggaaattgt ggaactgccg ccagaggaac gggcggtctt tctcgaagat
     2281 ctcggcctcc gagaatcggg attggagaaa ctcattaggg cgggctacga tcttttaggg
     2341 cttatcacct tttacacaac cgtcggcacg gaactccgcg cctggaccat tccccggggc
     2401 accagggcga atcaggctgc ggggaaaatc cacagcgata tggaacgggg ttttatcaga
     2461 gcggagatcc tccatttcga ggatttcaag gccgtgggaa atatcgccgt cgcccgggaa
     2521 aaaggactcg ttcgttcgga gggaagggat taccaaatta atgacgggga tatcgttgta
     2581 ttccggttca atgtataaaa aaaggtggat ggaaaaggag ggactaaacc atccacctgt
     2641 atataaactg aaagggtatt gcttatttcc ccttcagatc gccctttaag gggggcggtg
     2701 ggtggaaagg agggatataa accacccacc ggcattgaaa ccatttttaa tcgcaggatg
     2761 aatcaggcag gaagaagtct tccctttttt cttccttcac ctcgttcttt ttgtaactaa
     2821 cttctcgttc tgttaaccct tccgcttttc tgccgctatc tacggagaga aaaaaatcat
     2881 gtactatttg cctatcaagc ctgaaatact ctgaatcctc ctcactgcct gaatcctgct
     2941 gcgaatgatt cttttcctcc acgctcatga ttttgatctt tttgacaata agaagtagca
     3001 atcatcgtgc catgatgact ggaatggttt taaataatta tttaactttt agatatataa
     3061 ttagaactaa ttacttactt gttttatgct tacaaaacta acaatatgaa ttcaggtgat
     3121 aacttaggga ttgtaattcc ggagtaatga gcgtgcgtat atcgaacact aatattaggt
     3181 aaggaactgg gaaagctcct tccacaggcc ctctggatac tgtgcgcata acgcactata
     3241 tgtgcataaa gcgcacgcac aaaaattgcc gccagtaatt acgaaggagc ctcttgcttc
     3301 tcttcttgat acttcttctg ctcccggata agtttgtagc gggaaattcc cagttgccta
     3361 gctgcttccg ttttattgcc gcctgatttc tgaagggcgt tttccagaat ctttccctta
     3421 acatccttga gaattttttc agttaccgta tcgtaatcga tacctgtcaa aggcagttgc
     3481 tggaggaacg gctcaatctt tatatccaca tctttattgg ccagcatgtt taattccgtc
     3541 ggcatgttat cgggggagat gacattccct acccccttga ggatcatgat ccgttcgaca
     3601 atattcttca attccctgac attgcccggc caggagtacg cctgaagtat ggcttccgct
     3661 tcccggttga atcccttgac acgctttgaa aattttttat tgaactcctg aacgaaataa
     3721 cgggccagca taattatgtc atcggaccgt tcccggaggg gcgggattat aacaggaacc
     3781 acgcttatac ggtagaagag atcttcccgg aaggtgccgg cggcgaccat ttcactcaga
     3841 tcccggttgg tggaaaaaac gaaccgcaca tcgatgggga tgttctcatt ccctcccaac
     3901 cgccggatgt gaccgtcctc gagcatgcgg agaaatttcg cctggagttg aatcggcatt
     3961 tccccaattt catcgagaag catcgtcccg ccgaaacaat actcaagcag cccaattttt
     4021 ctctttctgg catccgtaaa ggcacccgcc tcgtatccga ataattcgct ctccagaagc
     4081 gtagcgggaa tggccgcaca atttatatcc acaaacggct tcaggtttcg gtgcgaatgg
     4141 cgatgaatcg cccgggcgat cagttccttg ccagtgccgc tctccccttg aataaggatg
     4201 ttcgtgtcat gtcccgccac atcggcgata acatgaaaaa tatctatcat cttccgcgat
     4261 ttgcccagaa tatcgtggaa gcccatgaga tgatcctccc gctccttcaa cttctcgacc
     4321 tccgtcttca gcgtttccgc ctccagggag agaagggaat gcataatggc attgtcatag
     4381 gccgtcgccg cgttcttgat gaggacatcc aggatattga cctgatcgga gctgaaactg
     4441 cctttttttt tcgccaacag catggcaccg acaaacttat cccggatgtg aaaaggtatg
     4501 gcgatttcca tcggaaaatc tgggaacaag ggttggattc ctttaccttc cattgctgtg
     4561 gtgacgaccg tggtgttttt ttcgattgct tcccggatga ggctggcact cattgtcgta
     4621 tcgcattcct gcttgctttt caccaccacc cgttcgccgt cattgatcaa gcagcgatcc
     4681 ttttctatgt cccacacata acccatgacc cgatcggcat aggcgatttt cagggcactg
     4741 atgaaaacta tttcgagaat gcgattgtcc agagagtaac tctggttcat ctccgaaacc
     4801 gcctcctgaa gaatcatcaa gtgcctaatc ttttcggcgt ttgtttccaa taaccgtgca
     4861 ttgtggataa ttacactgac ctgattggaa aaagtcagaa acagattgat ctcctcagca
     4921 aagaaatcga cctcctcctg gcgccatgcc gccaaggtgc cgatgacgta atccccgatt
     4981 ttaagggggg tgcagacgat tgacccacgg ccgacgatat tggtcagcat acggtccata
     5041 ggggtaatac ggggatccgt ggcggcattt ctgatcatga tcatatctcc ggtccgcgcc
     5101 actttggtcg ccagacagtc atgctcgttg atattcaggg gggtgctaaa ggcccggatg
     5161 gtctcttcct caaggtaatt tttgactacc ttcgtcacca ggtactgctg agtttcgtcg
     5221 cagagtctga tgatgccgcg ctcaaaacct acggcatcga cgagttcatc aaggatcttc
     5281 gccataatct catcctgatt ggcctggatc gtcagaaggt tgctgatccg gaagatactc
     5341 ttgaagaggc gatctttgtc gagaaccggt cccgcaaccc catctgacag ttcaaccatc
     5401 ccatcggtta cagagggcat cgtactatat gccgacattg aatttttact ccttatccct
     5461 atagctcttt gagtatgata ctcagggccg ttgtcagctt gactgtctct tcatctgcct
     5521 tcctcaggcg ggtgctgacg attctggcca tattcaagag gatgcgaaag ccgagatcat
     5581 atttatcttc cgccagttgg agaaaatcgt ctttgctaaa ttcatacaga tcacagttgg
     5641 ttacagcttt tatcgttgcc gttcgctttt gtgcctccag gagggcgatt tctccgaata
     5701 ccgcatgatc tttggcatcc agcctggtaa agaccttgtt tctccccgca tcctgctcgt
     5761 cttccaactc cccgataacc aggctcttga ccacttccac cgttccagac atcatgatgt
     5821 acatggtacc gccaacctcg ccctctctca tgatcacggc cccggccgga aacgttacgg
     5881 tacgagcaat gcccaggacc tcaccgatct gttcgggtga aaggcctctg aatatttccg
     5941 tctgctgcaa catggccgct tcctgtatca tgatctgacg tctcaaaata atcggaaggg
     6001 tctacgacga cggcatggtt cgggagagaa ccacggccag atcgtccgcg gcgatcatat
     6061 aatcgccttc gggattgata ttgattttca gggcatcctt ctccgtggaa aaatccttat
     6121 ttgcctcccg tatccgttct ctgatgaagt tgtcaatcac tgaggtattg ccggagagga
     6181 tatcctccag tttgatcgcc ttcctttcct tcatgatccc gatgagaatc gcctgctccc
     6241 gttcgcggtt atgagccgcc aattcctgga aaggtcgatt aatgaacccg gcgggtatct
     6301 gcgtccgcca taacttgttt ttgtcaccaa gggaaacaag actggatatt accctcggaa
     6361 gcccggggga attgatggcg ctcgccagga gcgcgccgac atgttcaccc cgaacaacaa
     6421 tttcatcgac attggcccgc ctcaaatggg ggcggttgtc cccgtccagg atttcggcga
     6481 tggtctttac gtgtggggca agcgatttca cggtcagggc tgccagcgcc gttcgtccgt
     6541 cgctccgttc ttgggaatga ccgccggatt gatcggccat gattataacg aattttgcct
     6601 atatcacatt ggctcggagc agcgcctctt cgtgggcgta atcgccccga aggaatttca
     6661 gcttgaagcg gctatattta gagcttattc gtaaataaaa tttgctttac gtaacaatat
     6721 cggttataat tact
//
LOCUS       DAOSMF010000100         6730 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_12500_length_6730_cov_18.527790, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000100 DAOSMF010000000
VERSION     DAOSMF010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 6730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6730
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12500_length_6730_cov_18.527790"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1490)
                     /locus_tag="PLT63_08720"
     CDS             complement(<1..1490)
                     /locus_tag="PLT63_08720"
                     /inference="COORDINATES: protein motif:HMM:NF011068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin biosynthesis protein"
                     /protein_id="HOD98845.1"
                     /translation="MVKRNVYLAMKDLREAKEIFSGVWRGKKTLPERINTEDALGRVT
                     ACPVYAGVSAPAYHSAAMDGIAVRAEMTYDATEQLPKVLTTGEDAVWINTGQALPEGY
                     NAVIMVEKIHELDEGHLEIRSPAYPWQNIRKVGEDIVATQLLFPQNYLIRPYDMGALV
                     AAGVFTVMVRRQPRVIIIPTGAEIISPKDVTDFRQLRNNRIIDSNSITLAGLIREAGG
                     APRIFPITPDEEEAIRDALLKAADSDADLILINAGSSAGSKDYTANILADIGEVLVHG
                     VAMMPGKPTILGIIGGKPIIGIPGYPVSAILSFEQFAKPLLLNLQGLKPRKSRTITVR
                     PSRNIPSKLGTEEFVRVNIGRVGDLSIATPLPRSAGSITTLTRAEGIIRIPPLSEGIG
                     QEEETIAELLVDDDDLINTVVIIGSHDITLDVLADEVRNASGRNIRISSGNVGSMGGL
                     IAIRKGICHIAGLHLLDTETGEYNVPYLRRYLKGIRISLFHLVLRE"
     gene            complement(1484..2734)
                     /locus_tag="PLT63_08725"
     CDS             complement(1484..2734)
                     /locus_tag="PLT63_08725"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HOD98846.1"
                     /translation="MFFKVKTSEEVLEILKGFDPVGTEVVHIEDALNRVLSGQIVSEE
                     DMPNFSRSSMDGYAVRAKDTFGASDSLPAFLELKGEVIMGGTPTDRVVPGTAVRISTG
                     GMIPEGADAVVMIEHCQSMDDKTIEVCRSVAPLENVIARGDDLRAGTIVFGKGSILRP
                     QDIGLIAGLGVETIPVYKRPTVGIISTGDEVIPIDQRPRPGQVRDINSYTLGAFCRQR
                     GAIPMNMGVCKDDFDEMRETVGRVLNACDTVWISGGSSVGARDMTVKVLESFAEARLL
                     VHGIAISPGKPTIIARIGAKALFGLPGHVASAMVVAEIFLAPFLSVLSGERRSSGDGH
                     LYRQAELERNVESASGRDDYIRVRLNKREGRLFAEPIFGKSGLISTLVDAQGLVKIDR
                     NTEGIYQGQTVEVMLFTPSRGEAW"
     gene            complement(2719..3126)
                     /locus_tag="PLT63_08730"
     CDS             complement(2719..3126)
                     /locus_tag="PLT63_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HOD98847.1"
                     /translation="MNIVTEDPLDTTNLFARLGKRNAGSVIFHYAVVKGQAGEKASAG
                     IRFERGGDVEGEMADIETDMRNRWHIDDILLVRRIGLLQVGDLISLVAVSSEASKDAF
                     EACQYGLSRIRKMGSLKKTELILDRGGNHCSSR"
     gene            3313..3522
                     /locus_tag="PLT63_08735"
     CDS             3313..3522
                     /locus_tag="PLT63_08735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HOD98848.1"
                     /translation="MKVSARNTLKGKVKKVTMGPVNAEVDIELPGGTEIVSIITKVSA
                     EALGLKKGMEVYAVIKASNVMVAVD"
     gene            complement(3646..4698)
                     /gene="modC"
                     /locus_tag="PLT63_08740"
     CDS             complement(3646..4698)
                     /gene="modC"
                     /locus_tag="PLT63_08740"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HOD98849.1"
                     /translation="MELRVAAQKKLGTFSLKMDFTIVGDRIGIFGPSGCGKSTLMGLI
                     AGLHHPDRGLITIDGQPVFDSGERVNIPAEHRRIGMVFQRPHLFPHLSVKGNLLYGYR
                     RCPPDQRKITLENLVDVLQIGDLLQRSVRNLSGGEQQRVAIGRAVLANPRMLVMDEPL
                     SALDDSLKFQIIPFLKRSCEAFAIPYLFISHSLTEMRIMTDQVLIVNEGSDAGQMTAE
                     GLARDAMGKSPDPYTNLLQLRKLRNYDGLFAYRWGDNELLISGDGQQDALYELSSTDI
                     ILFKRHPEATSARNLLQCRVGEMFAAGNRIGIVLLCGGEKLVAVIVKEAAQDLEIRNG
                     MEVFAAIKATAFRHLG"
     gene            complement(4700..5386)
                     /gene="modB"
                     /locus_tag="PLT63_08745"
     CDS             complement(4700..5386)
                     /gene="modB"
                     /locus_tag="PLT63_08745"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HOD98850.1"
                     /translation="MGIFSESDYLAILLSMKVAVVATILSLPFGFAVAYVMTYRKFRG
                     RVALDVVVNLPLTLPPVVIGYLLLLLLGKNGWIGMWVLEPLGIKVIFTWKAAVIATAV
                     VGFPLMVRSIRTGMEAIDEKLIQASRTLGARWFDTVVTVILPLSFRGIIAGSILMFAR
                     GLGEFGATIILAGNIPGVTQTIPLAIYEYVSSPSGDTVAVALCVVSVTISVAVLIFHE
                     WLGRRIVRVD"
     gene            complement(5393..6151)
                     /gene="modA"
                     /locus_tag="PLT63_08750"
     CDS             complement(5393..6151)
                     /gene="modA"
                     /locus_tag="PLT63_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOD98851.1"
                     /translation="MKRTIRRFFVVLSILGLVLLTVPAYAEDIRVSAAASLREVVTEL
                     TANYTKTHPEVKFLNNLGASGALAKQIENGAPADIYFSANLEWMDYLKVKKLLDEKSI
                     GVLAYNSLVFVGMPDAKAKRIEDVPMLEKIAIGSPKSVPAGDYAMKALKKAGIDKKLA
                     NKLVMAKDVRECLMYAEKGEVSGAFVYKTDALQMAKKAKILFVVPQELYPRVTYPVGL
                     TISGSKKADAVAFHKYLQSTEAKGVLTKYGFIAK"
BASE COUNT         1458 a         1905 c         1837 g         1530 t
ORIGIN      
        1 tgctctcgga ggacgaggtg aaagaggctt atcctgatgc ctttcaggta tctccgcagg
       61 tagggaacat tgtattctcc cgtttccgta tcgagaagat gcaagcccgc gatatgacag
      121 atccccttcc ggattgcgat cagccccccc atgctcccca cgtttcctga cgaaatccgg
      181 atattccgtc ccgaggcgtt cctgacctcg tcggcaagga catccagggt gatgtcatgg
      241 ctgccgataa tgacgacggt attgatcaag tcatcatcat cgacaagaag ctcggcgatt
      301 gtttcttcct cctggccgat gccctccgag aggggcggga tgcggatgat cccttccgct
      361 ctggtgaggg tggtgatgga acctgccgat ctgggaaggg gcgtggcgat gctcaggtct
      421 ccgacccggc cgatgttgac gcggacgaat tcttccgtgc ccagcttgga ggggatgttt
      481 ctcgatggcc tgacggtgat ggtgcggctt ttacgaggct tcaagccttg gaggttcaag
      541 agcaggggtt tggcgaactg ctcgaaagat aggattgcgg aaacggggta tcccggaatc
      601 ccgatgatgg gcttcccgcc gataatgccc aagatggtcg gtttccccgg catcatggcc
      661 accccgtgga cgaggacttc ccctatatcc gccaggatat tagcggtata atccttggag
      721 cccgccgatg atccggcatt gatgaggatc aggtccgcat cggaatccgc ggcctttaaa
      781 agggcatccc ggatagcttc ttcctcgtcg ggggtgatag ggaagatccg gggcgccccg
      841 cctgcctcac ggataagtcc cgccagggtg atggaattcg agtcgatgat ccggttgttg
      901 cgtaactgac ggaaatccgt gacatccttg gggctgataa tttccgcccc cgtggggatg
      961 atgatcaccc tgggctgcct ccggaccatc accgtaaaga cgccggcggc gaccagcgcc
     1021 cccatgtcgt agggccggat cagataattc tgcgggaaga ggagctgggt ggcgacaatg
     1081 tcttccccga ccttccggat attctgccag ggataggcgg gcgaccggat ctcgaggtgt
     1141 ccctcatcca gttcatggat cttctccacc atgatgacgg cgttgtaccc ctcgggcagg
     1201 gcctggcccg tattgatcca cacggcgtct tcgcctgtcg ttaatacttt cgggagctgt
     1261 tccgtggcgt cgtacgtcat ttccgccctg acggcgatgc cgtccatggc cgcggaatga
     1321 taggcaggtg cggaaacccc ggcataaacg gggcatgcgg tgactcgccc caaggcgtct
     1381 tccgtgttga tccgttcggg gagggttttc tttccccgcc atacaccgga aaagatctcc
     1441 ttcgcctctc ggagatcctt catggccaga tagacgtttc ttttcaccat gcctctcccc
     1501 gggagggagt gaagagcatc acctcgacgg tctgcccctg gtagatccct tccgtgtttc
     1561 tgtcgatctt gaccaggccc tgggcatcga ccagggtgga gatgaggccc gatttgccga
     1621 agatcggctc cgcgaagagc cgcccctcac gtttgttcaa tctgaccctg atgtagtcgt
     1681 ctctcccgct tgccgattcc acattacgtt caagttcggc ctgccggtat aaatgaccgt
     1741 ccccggacga ccgcctttcc cctgacagta ccgatagaaa aggggccaag aaaatctccg
     1801 ccacaaccat ggccgaggca acatgaccgg gcagcccaaa cagggccttc gcgccgattc
     1861 tggcgatgat tgtcggtttt cccgggctta tcgcgatgcc gtgaacaagg agtcgcgcct
     1921 cggcaaaaga ttcaaggacc ttcacggtca tatcccttgc ccctacggag cttccgcctg
     1981 atatccatac ggtgtcgcag gcattgagaa ccctgccgac ggtttctctc atttcgtcaa
     2041 agtcgtcttt gcataccccc atgttcatgg gtatcgcccc cctctggcgg cagaaggcgc
     2101 ccagggtata gctgttaatg tcccgcacct gtccgggcct ggggcgctga tcgatgggga
     2161 tgacctcgtc ccccgtggag atgatcccga ccgtgggtct tttatacacc gggatcgtct
     2221 ccacgcccag acccgcgatg aggccgatgt cctggggcct gaggatggag ccttttccga
     2281 agacgatggt cccggctctt aaatcgtcac ccctggcgat gacattttcc aggggcgcca
     2341 cggaccggca cacctcaatg gttttgtcgt ccatgctttg acagtgctcg atcatgacca
     2401 cggcgtcagc tccttccggg atcattcccc ccgtcgatat cctcacggcg gttcccggaa
     2461 cgacacggtc cgtgggcgtt ccacccatga tcacttcccc tttcagctcc aggaaggcgg
     2521 gaaggctgtc ggaagcgccg aaggtgtcct ttgcccgcac cgcatagcca tccatggaag
     2581 accgggaaaa attcggcatg tcctcttcgg aaacgatctg tccgctcagg acgcggttaa
     2641 gggcgtcttc gatgtggacg acctccgtgc ctacggggtc gaagcctttc aatatctcca
     2701 ggacctcttc ggatgtcttc accttgaaga acaatggttg cctcctcggt ccagaataag
     2761 ttcggttttt ttgaggctcc ccatcttcct gatcctggac agaccatatt ggcatgcctc
     2821 gaaggcatcc ttgctggcct ctgaactgac cgccaccagg gagatcaaat cgccgacctg
     2881 caagaggccg atgcgccgga cgagcaggat atcgtcgata tgccagcgat tccgcatgtc
     2941 cgtttcgatg tccgccattt caccttccac atcgccaccc cgctcaaagc ggatccccgc
     3001 tgacgctttt tcgcccgcct ggcctttcac gacggcataa tggaagataa ccgatccggc
     3061 attccgtttg ccgagccttg cgaacaggtt ggtcgtatcc aaaggatctt ctgtaacgat
     3121 gttcatctcc gcctccattt tttgccattt ttatcaaatt taccatgaaa tgtgaatgga
     3181 aagaattccc catgcccgcc atgcattcat tgacaaggaa ggatttgact gctacctata
     3241 tacaaatagt cgtcttaaac gtaaacagtt gtgttggtcg tgtttcggag atctcaaacc
     3301 aggaggtgaa aaatgaaagt cagcgcacgt aacacgttaa agggcaaggt caagaaagta
     3361 acaatgggtc ccgtcaacgc ggaagttgac attgagcttc ccggcggtac cgaaatcgtc
     3421 tcgattatca ccaaggtttc agccgaggcc ctcgggctta aaaagggtat ggaggtttat
     3481 gcggtcatca aggcatccaa tgtcatggtg gctgttgatt agccgatcct ggtcgggatc
     3541 gttttcggac ggtcatggtt atgtttgcgc aggggctgac accggcatgg acgactgcca
     3601 ggggcagccc cttgcttgtg tctgggaccc ggtaacattt gcggctcacc ccaggtgtct
     3661 aaaggccgtt gccttgatgg cggcgaaaac ctccatgcca ttcctgatct ccaggtcctg
     3721 agccgcttcc ttcacaatca cggcaacgag cttctccccg ccgcataaga gcacgatgcc
     3781 gatcctgttt cccgccgcaa acatttcgcc cacccggcat tgcaagaggt tgcgggcact
     3841 ggttgcctcg gggtggcgct tgaaaaggat aatgtccgtg gaagaaagct cataaagggc
     3901 gtcctgctgc ccgtcccctg aaatcagcag ttcattgtct ccccagcgat aggcaaataa
     3961 accatcataa ttgcgaagtt ttctcagttg caggagattc gtgtatgggt ccggactttt
     4021 ccccatggca tccctagcca gtccctccgc cgtcatttgc ccggcgtcac tcccttcgtt
     4081 tacgatcaac acctgatcgg tcatgatcct catttccgtc aaggaatggg aaatgaaaag
     4141 ataggggatg gcaaaggcct cacaggatct cttgagaaaa gggatgatct ggaacttgag
     4201 gctgtcgtcc agggcggaaa ggggttcgtc catcaccaac atgcggggat tggcaagaac
     4261 tgcccggccg attgctacgc gctgctgctc gcctcccgag aggttacgca cactccgttg
     4321 cagaagatcc ccgatctgca gcacgtccac cagattctcc agggtgattt tgcgctgatc
     4381 cgggggacaa cgccggtagc cataaaggag gttccccttg acggagaggt ggggaaaaag
     4441 atgggggcgt tgaaagacca tgccgatgcg tctgtgctct gcagggatat tcactctttc
     4501 cccactgtca aaaacgggct gtccgtcaat ggtaataagc cctcggtccg gatggtgcaa
     4561 cccggcgatg agacccatga gggtggattt gccgcaaccg gaggggccga agatcccgat
     4621 ccggtcgcca acaatcgtga aatccatttt caaggaaaac gtcccgagtt ttttttgggc
     4681 ggcgactcgc aattccatat cagtccaccc tgacaatacg cctccccagc cattcgtgga
     4741 agatcaaaac ggcgacggag atggtgacgg atacgacgca cagggccacg gccacggtat
     4801 ccccgctggg ggaactgaca tattcataga ttgccagagg aatggtctgc gtcactccgg
     4861 gaatgttgcc ggcgagtatt atcgtggctc cgaattcacc taacccgcgg gcgaacatca
     4921 ggatggatcc ggcgataata ccgcggaaag aaaggggaag aatgactgtt acaaccgtat
     4981 caaaccatcg ggcacccaag gtgcgggatg cctggatgag tttttcatca atggcctcca
     5041 tacccgtcct gatcgaccgg accatgagtg ggaaacccac tacggccgta gcgataaccg
     5101 cggccttcca ggtaaaaata acttttattc ccagcggttc cagtacccac atcccgatcc
     5161 agccgttctt tcccaacagc aggagcaaga gataaccgat gacaacgggt ggcaaggtca
     5221 ggggtagatt caccaccaca tccagggcga cccgtccccg gaacttccgg tatgtcatga
     5281 cataggccac ggcaaagccg aagggtaggg acaggatggt cgccactacg gccaccttca
     5341 tggacagcag aatggcgagg taatccgatt cgctgaaaat gcccatgaag gattatttgg
     5401 cgataaagcc gtattttgtg agcacccctt tcgcttcggt ggattgaaga tacttgtgga
     5461 atgccacggc atcggccttt ttgcttcccg agatcgtgag cccaacggga taggtcacgc
     5521 gaggataaag ctcctgggga acaacgaaaa gaatcttcgc ctttttagcc atttgcaggg
     5581 cgtcggtttt gtaaacaaag gccccggaga cctcaccctt ctccgcgtac atcaggcatt
     5641 ccctgacgtc cttggccatg accagtttgt ttgcaagttt tttatcaatg ccggcctttt
     5701 tcagggcctt catggcataa tcacccgcgg gaacactctt cgggctgccg atggcgatct
     5761 tctccagcat gggtacatcc tctatccttt tcgccttcgc atcgggcatc ccgacaaaga
     5821 ccagggagtt gtacgccaaa acgccgatgc ttttctcatc cagcagtttc tttaccttta
     5881 ggtaatccat ccattccaga ttcgcggaga aataaatgtc cgccggggcg ccgttttcaa
     5941 tctgtttggc gagggctccc gaagccccta aattgttcag gaacttaacc tcggggtggg
     6001 tcttggtgta attcgccgtc agttccgtga cgacctcgcg caggcttgcc gcggcggata
     6061 ccctgatgtc ctcggcgtag gcgggcacgg tcaacaaaac cagtcctaaa atggacaaca
     6121 caacgaaaaa ccttctgatt gttcttttca ttcccggtgt cctccgaacc gatgattaat
     6181 aagataatat ggtcgaaaag atgctacttt ttacccttaa gtatagaggg gcagtcttct
     6241 caagtcaagg aatatttcga tccgcaggcc tgaaagtaca caaaaaccgg acgaaccata
     6301 cacggcagag gcatcataag agatggacta caaaggggaa attgatgtta tccactgagc
     6361 gagtatttgc tattttaaac ttatgccgta tttagcatat tttttcttga cgtgccgccg
     6421 cattaccctg gaagggggac ggatcaagaa agatgagata tcggcatcgc aacacgttct
     6481 ctttgaatct tcttgctgct cttgatctcg gagcgaaaga cagtcgtccg ggaacagcgc
     6541 ccccggcgcg ctgtctaacg tcggcggacc cacctggagg gaaagcaaac gagcctatgc
     6601 cgtgacttta tccattgcgg cagccagggc cagttgatgg ttcgtctcga cgaccggcga
     6661 cccctgatgc gcttcaggtc gggtgcggag gttgggagaa agagagaacc accttccagt
     6721 cctggaacag
//
LOCUS       DAOSMF010000101         6729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_12503_length_6729_cov_16.609979, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000101 DAOSMF010000000
VERSION     DAOSMF010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 6729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6729
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12503_length_6729_cov_16.609979"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /gene="traN"
                     /locus_tag="PLT63_08755"
     CDS             <1..346
                     /gene="traN"
                     /locus_tag="PLT63_08755"
                     /inference="COORDINATES: protein motif:HMM:NF018666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="conjugal transfer protein TraN"
                     /protein_id="HOD98852.1"
                     /translation="GSYLGFNLCHFVGDLKTGNFLGMKLKSENVYCCFNSILARLVHE
                     QGRPQVSRGWGSSEQPDCTGFSIGEFAALDFSQMNLGEYMQYVQTKTNISPAEMEAIQ
                     QRAITVTGAGGS"
     gene            complement(350..1738)
                     /locus_tag="PLT63_08760"
     CDS             complement(350..1738)
                     /locus_tag="PLT63_08760"
                     /inference="COORDINATES: protein motif:HMM:NF014581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HOD98853.1"
                     /translation="MDDLDALLELNDKRSVMRHHDGKTRLGWGVNLNDIMKEKEIFIK
                     DSERSGHFGCFGTTRIGKSKLLENIVEQDIKKGYNVVIIDPKGDPELLSRVCQAAAEC
                     GRLREVMMLNPIFPDSSIMLDPLAYYYMEEELVNHIVSGIKAKEDYFIAVAQEVSHAV
                     IAGFALKQRHKNAMEGKNEPMSINFLQVKQNSSWHTLSRFKESIQNLPGSEDLVASME
                     QTLSSPQEFFAKVASSLRTTLSALTSGTTGRIIGKAKHNEFVRRIESGEGAILFCNTG
                     SMLTDRTAHILGRVLLSMIKSMVGRVFASSRKLDPPLCLHLDEGHNVLYRGVQELFSK
                     FGGANVWIHFYTQSISQMEEEVGPEVTTSIIDNINTWMYMLVNHPATAQYMEDSSPTK
                     KKFQSILSFGGGISLRETEERQILAAKVLQLPKRHFYLRTYGELYRGVTHDVRPLEVE
                     IEMPRIVGSSPV"
     gene            2033..3082
                     /locus_tag="PLT63_08765"
     CDS             2033..3082
                     /locus_tag="PLT63_08765"
                     /inference="COORDINATES: protein motif:HMM:NF024554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HOD98854.1"
                     /translation="MGNKERRSGGLLEKINRLWQIMTRPNPRQHRRAKERISDREIEE
                     ARRVNLAHLIESLGHKPRWFSAEKAMFSSPLREEKTPSFTVSYYKGRWGWKDWGTSES
                     GDTIAFVQAYYGLGFIEAVRKLSGGTNLSVPSSAPAPSARSEYDEDKIKFVRKLHSDR
                     MSLMSPKEHRIVRLYFEDKKVIFYPEIAVVMYHSFKDKKSFVGIPMPHARCIKGLECR
                     EIGGTDRKTLGNKTLWYLGRKSSKVLVTESILDALAGEIVLSDSTVSLMSINGVGNVG
                     KLDGVLAKLRPKEVLLALDNDAPGREAQIAAVQIASRYADRVIQVDHHIRAGVKDLHK
                     LLLPAQQSTPVAKAE"
     gene            3779..4726
                     /locus_tag="PLT63_08770"
     CDS             3779..4726
                     /locus_tag="PLT63_08770"
                     /inference="COORDINATES: protein motif:HMM:NF019348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOD98855.1"
                     /translation="MQESISTIFEGVSAPASLMMEVREDTAGHPMIPRRKLYRFRQEP
                     MSDFIGWLKGAGGYDPLLITGPTASGKSSLAEQVAARLNQPAYIVPCHERMEVPDLFG
                     RFVVRDGQMVWVDGPVIAGLKDPAGAWIIMDEFDSMEPGALLGINPVLEGRKFIVPET
                     GEFIDPVANGAKIICTGNTAGMGDQTGEYLATKRQNVATMGRFAVMEMAYPTEEEELD
                     ILSLHRPDLPAKIGEHMIKVASLAREQYIAGEMQCVFCTRSLIRWAHLAIYFRKKKGV
                     NYLMHALDRAIGFKYEPVVREALHQFTQRIFGNDAITIN"
     gene            4823..5125
                     /locus_tag="PLT63_08775"
     CDS             4823..5125
                     /locus_tag="PLT63_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98856.1"
                     /translation="MSGGKDWIGVACPDRFIAFWGKTGTINQSKTRKVIGTPVSHLTA
                     VMAGKVQKGYEVIAELHDPLPPQSSNPAAPPPSKKQSAIAKIVAAWRDEAPDDGCF"
     gene            5138..5575
                     /locus_tag="PLT63_08780"
     CDS             5138..5575
                     /locus_tag="PLT63_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98857.1"
                     /translation="MYQSINPPENAEWGICLKRFGKYWIAFSTDKLFLTIGDIPPHKV
                     TGRFPKSPEIITAMLKKKEGQGYRVVAEWKPDTQWVPHEAATPTTQTLPPPRGGGGKY
                     GKHHQLPTPPPNRPVPPIPPPKPMTIIDLVRKWRDEAPDDECF"
     gene            5745..>6729
                     /locus_tag="PLT63_08785"
     CDS             5745..>6729
                     /locus_tag="PLT63_08785"
                     /inference="COORDINATES: protein motif:HMM:NF022792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3150 domain-containing protein"
                     /protein_id="HOD98858.1"
                     /translation="MNENILSNIVLLNLDIRLWSGRRRLAPEDLKLADGSELPPESLA
                     RLGSKRVIDPERINELYQIRQEAHRIALRHGARLMGGYAIPTGRMAELKDQLDALVAL
                     GRKLKAELLSDLSAAVEEWKEKNPGWEALIDRADINPETVGRAISFSWQVYNIAPVEG
                     QEEGLEAEINGLSGQLRQEIQTEARLLWKNSVQGKAEVTQRTLRPIRAMGKKLNGLAF
                     LEPDLEDLVKEISDVLETLPKKGPIRGRHLAAVCGIVGLLGNIPDAKTMAEAAVKAEE
                     EAAQKAIALKQQDSLLGSVETAVTETTDATEIPPAFVIQGAGEIYAGAAPPP"
BASE COUNT         1510 a         1871 c         1958 g         1390 t
ORIGIN      
        1 aggttcatac ttggggttca acctttgcca ttttgtaggc gatctcaaaa cgggcaattt
       61 tctcggcatg aagctgaaga gtgaaaacgt ctattgctgc ttcaactcta tccttgcccg
      121 cctggtccat gaacaaggcc ggccccaggt cagtcgtggg tggggatcgt cggaacagcc
      181 agattgtacg gggttctcca ttggcgaatt tgccgctttg gacttttcgc aaatgaattt
      241 gggggagtac atgcagtacg ttcagacaaa aacgaacata tcccccgccg agatggaggc
      301 cattcagcaa cgagcaatta cggtgacagg cgcgggaggc tcgtagcaat caaaccggcg
      361 aggatcccac gattctcggc atttctatct caacctctaa tggcctgaca tcgtgcgtga
      421 ccccccggta aagctcgcca taggtccgca ggtagaagtg gcgtttcggc aactggagca
      481 ccttggccgc caatatctgc ctttcttccg tctcccggag ggatattccg cccccaaagg
      541 acaggatcga ctggaatttc ttcttcgtag gagatgaatc ctccatgtac tgtgccgttg
      601 cagggtgatt cacgagcatg tacatccagg tgttgatgtt gtcgatgata ctggttgtca
      661 cctccgggcc gacttcttct tccatctgtg aaatgctctg tgtgtagaaa tgaatccaga
      721 cgttggcgcc gccgaacttg ctgaacagct cctgcacgcc cctgtacagg acgttgtgcc
      781 cctcatctaa atgcaggcat aaagggggat cgagttttcg actggacgca aatactcgtc
      841 cgaccatgct cttgatcatg gacagcaaga ctctaccgag aatgtgggcc gttcgatccg
      901 tcagcatgga acctgtgttg cagaacagga ttgcgccttc tcccgattct atgcgcctga
      961 cgaattcgtt gtgtttggcc ttgccgataa tcctccccgt cgtacccgat gtcagggccg
     1021 aaagtgtggt cctgagcgac gatgctactt tggcaaaaaa ctcctggggc gacgacaaag
     1081 tctgttccat gctggccacc aggtcctcgc tgcccggaag gttttggatc gattccttga
     1141 accgcgacag ggtgtgccac gaagaatttt gtttgacttg gaggaaattg atggacatag
     1201 gctcgttttt cccctccatg gcgtttttgt gccgctgctt gagcgcaaaa ccggctatca
     1261 cggcatgcga aacctcctgg gccacggcga tgaaataatc ctctttggcc ttgattccgg
     1321 aaacgatgtg gttaaccagc tcctcctcca tataataata cgccagggga tcgagcatga
     1381 tggagctgtc cggaaatatc gggttcagca tcatgacctc ccggagcctt ccgcactcgg
     1441 cggcggcctg gcacaccctg cttaaaagct caggatcgcc cttggggtca atgatcacga
     1501 cgttgtatcc cttcttgatg tcctgctcta cgatgttctc cagcagtttc gatttgccga
     1561 tgcgcgtcgt gccgaaacag ccgaaatgcc cgcttctttc ggagtccttg atgaatatct
     1621 ccttctcttt cataatatcg ttcaggttta ccccccatcc caggcgggtt ttcccgtcat
     1681 gatgcctcat caccgagcgt ttatcgttta gttcaagcaa agcgtcgaga tcgtccatgt
     1741 tcagcctcca tcatttcctt tgctgtatat tacttccgcc ccgctgttcc ccttttcttt
     1801 cctctctctc cctttcggga agtattatat aggcaattag ttcgtttagt aggtttcgac
     1861 cgaaaagggc gcaagtcgcc attgatgcag agatgccaat ggatgatttg cgcctttttt
     1921 ttacgtttcg gtaagccctg acccttgtaa cgagggtggg cgcggaacgt ggcattaccc
     1981 cgccggtaag tcctgacatc cgcgaggatg caggcacggc aaaggaggtg gaatgggaaa
     2041 taaggaaagg aggtccggcg ggttgctgga gaagatcaac cggctgtggc agataatgac
     2101 acggccaaac ccccggcaac accgccgggc gaaagaaaga atctccgatc gggagatcga
     2161 ggaggcccgg agggtcaacc ttgcccacct gatcgaatcg ctcggccaca agccgaggtg
     2221 gttctctgcg gagaaggcga tgttttcttc gcctctccgg gaggagaaga ctccgtcgtt
     2281 cactgtctcg tattacaagg gacggtgggg gtggaaggat tggggaacca gcgagtccgg
     2341 agacacaatc gctttcgtcc aggcttatta tggtctcggc ttcatcgagg cggtaaggaa
     2401 gctaagtggc ggaacaaacc tttctgtccc gtcgtccgcc ccggcgccat cggcccggag
     2461 cgagtacgac gaggacaaaa tcaagttcgt gcggaagctg cacagcgacc gtatgagcct
     2521 gatgtccccg aaggaacacc ggatagtccg gctgtacttc gaggacaaaa aggtaatttt
     2581 ttatcctgaa attgcggtgg tcatgtacca ctccttcaag gataaaaagt cattcgtcgg
     2641 tattcccatg cctcacgccc gttgcatcaa agggcttgag tgccgcgaga tcggcggtac
     2701 ggaccggaag actctcggta acaagaccct gtggtacttg ggtcggaagt cttccaaggt
     2761 gctggtcacg gagtcgatcc ttgacgccct ggccggcgaa attgtccttt ccgacagcac
     2821 cgtatcgctg atgtccatca acggcgttgg caacgtcggg aaactggacg gtgtcctggc
     2881 gaagctgaga ccaaaagagg tcctgctcgc cctggacaat gacgctccgg gccgcgaggc
     2941 ccagatagcg gcggtgcaga tagcatcgcg ctacgcggac agggtgatcc aagttgatca
     3001 tcatatccgt gcgggcgtga aggacctgca taagctgctg ctgccagcgc aacaatcaac
     3061 acctgtcgca aaggcggaat aggcgggagt atctcatggc gaagttgtca ccgatacaac
     3121 cgccccttgg tgcaatttgg ggtgtccgaa ccgagggtta taacgcttcg ggtttcctga
     3181 aagacaccgg catgttctta actgccggcg gcgaaggggt ttatgttggg agccttcaca
     3241 acgaacccgg ctggctacgc cgggtaagac ccgttaaaac gagagatatt gaggggtaca
     3301 taatccgtta ccaggaaacg agagtgaatc ttggttatcc ggtcgtggaa atatggactc
     3361 cctaaaaggg ctggcatccc gccggccccg aggcagtcga ggttccgcga attcccatgc
     3421 ggaccagtct gtcaaccata atgaagcagt ggcaggcgca aggcgccgaa tgcttctgaa
     3481 aatccgggga ggcgtcctgc tacccccgta aaccatatca aaatcaaccc cctacaacct
     3541 gggcgtgctc ctgcgggaca ctttagcatc cgcaagggtg tgctcacagt tgcgggtggg
     3601 gataaaatcc aaacacatac gtgatttgtg ccgtgaagcg gcgctgagtt tccagggatg
     3661 agaaagcgat ttcgagagtc cttggcgccc acgcccccaa cagcggcgca ttgtctatcc
     3721 attaccccgc cggtaagacc tgaccttcgc gagaaggcag gcacggcaag gaggaaaaat
     3781 gcaagaatcg atttcgacaa tattcgaagg ggtcagcgcc cccgcgtcac tcatgatgga
     3841 agtcagggag gacacagccg ggcatccgat gatccctcgg aggaagcttt atcggttccg
     3901 tcaggagccg atgagcgact tcatcggctg gctaaaaggg gccggcggtt acgaccctct
     3961 cctcattacg ggtcccacgg cctccggcaa atcgtcgttg gccgagcagg tggcggcgcg
     4021 gcttaaccag cccgcctaca tcgtgccctg ccacgaacgg atggaggtgc ccgacctgtt
     4081 tgggcgcttc gtcgtccggg acggccagat ggtatgggtg gatggtccgg tcatcgcggg
     4141 gttgaaagac cctgccgggg cgtggatcat catggatgag ttcgactcga tggagcctgg
     4201 tgctctgttg ggtattaacc ccgttttgga gggacggaag ttcatagtcc ccgaaacagg
     4261 ggaattcatt gaccccgttg ccaatggggc aaagatcatc tgtaccggca acaccgccgg
     4321 gatgggtgac cagacggggg aatatctcgc gactaagcgg caaaacgtcg ccacgatggg
     4381 acgttttgca gtcatggaga tggcgtaccc cacagaggag gaggagcttg acatcctttc
     4441 cctccaccgt ccggacctcc ccgcgaaaat cggggaacac atgatcaagg tcgccagcct
     4501 ggcccgcgag cagtacattg cgggtgagat gcagtgcgtg ttctgcacgc gttctctcat
     4561 caggtgggcg cacctggcga tctatttcag aaaaaagaag ggcgtcaatt accttatgca
     4621 cgccttggac agggcaatcg ggttcaagta tgagccggtt gtccgggagg cgctgcacca
     4681 gtttacccaa agaatatttg ggaacgacgc cataacgatt aactagggga gggggtgaac
     4741 catgccttct ctttccactt ctgggtggcc ggtatccatt ccgtctgacg cggttgaggc
     4801 cgtttggcta cggagcgact ccgtgtccgg cggcaaggac tggatcgggg ttgcctgccc
     4861 cgataggttt atagccttct ggggaaagac tggcacgatc aaccagtcaa aaacaaggaa
     4921 ggttattggc acgcccgtca gccacctgac ggccgtaatg gctggcaagg ttcagaaggg
     4981 atatgaggtt atagcggaac tgcacgatcc tcttccgcca caatccagca acccggcggc
     5041 gccgccccct tcaaaaaagc aatccgcgat agccaaaatc gtcgcggcct ggagggatga
     5101 ggcgcccgac gacggctgct tctgaaagga gaggaagatg tatcaatcca tcaaccctcc
     5161 ggaaaacgca gagtggggga tttgtctcaa aaggtttgga aagtactgga tagcgttcag
     5221 taccgacaag ctttttttga caataggcga cataccgccc cataaagtca ctggccgatt
     5281 tccgaaatcg cccgaaatca ttacggcgat gctcaaaaag aaggagggcc agggctacag
     5341 ggtggtggcc gagtggaaac ccgacacaca atgggtgcca cacgaagctg caacccccac
     5401 aacccagaca ctcccccctc cccggggagg cggcggcaag tatgggaagc atcaccagct
     5461 cccaacgccg ccgcctaatc gtccagttcc tccaataccg ccgcctaagc ccatgacgat
     5521 cattgacctg gtgcggaagt ggcgcgatga ggcgccggac gacgagtgct tctaacgggg
     5581 ccgcaaggcc ccaaaatccc attaaattta aaggccaaag cctgggcgtg ctcctgcggg
     5641 acactttagc atccgcaagg gtgtgctcac agctacggca aacattaccc acgccgcata
     5701 agtcctgaac cagctatggc aggcacggca acggaggata aagtatgaac gaaaacatcc
     5761 tgagcaatat cgtgttgctc aacctggaca ttcgcttgtg gtccgggcgg cgcagacttg
     5821 cccccgaaga cctgaagctg gccgacgggt cggaactccc cccggagtcc ctggcccggt
     5881 taggatccaa acgggtcatc gacccggagc ggataaacga gttgtatcag atccgccagg
     5941 aggcccaccg aattgccctg cggcacggcg cccgtctcat gggcggatac gccattccca
     6001 cggggaggat ggccgagctg aaggaccagc tcgacgccct ggtcgccttg gggcgcaagc
     6061 tgaaggctga gcttctgtca gacctttccg ctgccgtaga ggagtggaaa gagaagaatc
     6121 caggttggga ggcccttatc gaccgggccg acatcaaccc ggaaacggtc ggacgggcca
     6181 tctccttctc atggcaggtg tacaacatcg cgcctgtcga gggacaggag gaaggtctgg
     6241 aggcggagat caacggtcta tccggccagc tccgccagga gatccagact gaggcccgcc
     6301 tcctctggaa gaattcggtc caggggaagg cagaggtaac acagcgcacc ctccgcccga
     6361 tccgggccat gggcaagaaa ctcaacgggc tggccttcct ggagcccgac cttgaggacc
     6421 tggtgaagga aatctccgat gtcctggaga ccctccccaa gaaaggtccg atccggggca
     6481 ggcacctggc tgcggtttgt gggattgttg gcctccttgg caacatcccc gacgccaaga
     6541 cgatggcgga agccgcggtc aaggccgaag aggaggccgc gcagaaagct atagccctga
     6601 agcagcagga cagccttctg gggtctgtgg agactgcggt gacggaaacc accgacgcca
     6661 cggagatccc cccggccttc gtcatccagg gggccggaga gatttacgcg ggagcggctc
     6721 ccccgcctc
//
LOCUS       DAOSMF010000102         6669 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_12640_length_6669_cov_21.741155, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000102 DAOSMF010000000
VERSION     DAOSMF010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 6669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6669
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12640_length_6669_cov_21.741155"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /locus_tag="PLT63_08790"
     CDS             <1..869
                     /locus_tag="PLT63_08790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOD98859.1"
                     /translation="RSLGRPYEIVMIDDGSRDGSLAILKDLVRENPELRVVELTRNYG
                     QHAAIMAGFSIVRGDIVVTLDADLQNPPEEIPRLVAEMENGGYDVVGTIRRRRRDSAF
                     RLGASRLINIVARRITGVHMTDWGCMLRAYRRPVVERMRECHEHATFIPALATVFAKR
                     LTEIEVAHEERHGGKSNYPLRKLINLQFDLVASFSDFPMKMLMYGGILMSVLGVCFGV
                     FLAVARLVYGALWAAQGVFTLFAVLFIFVGLQFFALGVIGEYIGRIYREVRRRPEYVI
                     EHIHGVRDRSGS"
     gene            866..>1590
                     /locus_tag="PLT63_08795"
     CDS             866..>1590
                     /locus_tag="PLT63_08795"
                     /inference="COORDINATES: protein motif:HMM:NF005414.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase"
                     /protein_id="HOD98860.1"
                     /translation="MKAVVFAYSNMGVTGLEALARHGFDILGVVTHRDDPEEKQWYAS
                     VAAWAEGRGLPVCRPEDVNAPEWTARIAALAPEVIFSFYYRKMLRSEILALAPAGAFN
                     LHGSLLPAYRGRVPVNWVLANGEERTGVTLHWMTARADAGDIVSQRAVAIDFDDTAFT
                     LYGKLCREAALLLDETLPLIRSGRTPRIPQDHARATCFGGRKPADGRIDWNRPARRIY
                     DLIRAVADPYPGAFTFLPDGTKML"
     assembly_gap    1591..1690
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1691..2124
                     /locus_tag="PLT63_08800"
     CDS             <1691..2124
                     /locus_tag="PLT63_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98861.1"
                     /translation="ADPYPGAFTFLPDGTKMLIWWGLPEPVATPPADGGAALETAPAA
                     AGTPLAFPGQAAGPPSGAGGNGEGVLSGATPAGTEGRDKNGSPGDPPPGLVLVDERRE
                     VYVATGTGRLRLLHVEIAGKRMSDEEIWQYFRDLGGVIVLG"
     gene            2246..3292
                     /locus_tag="PLT63_08805"
     CDS             2246..3292
                     /locus_tag="PLT63_08805"
                     /inference="COORDINATES: protein motif:HMM:NF008872.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-keto-pentose/UDP-xylose
                     synthase"
                     /protein_id="HOD98862.1"
                     /translation="MKILILGVNGFIGHHLTNRILATTDWRVYGMDLSEDRLESALCN
                     PRFQFLEGDIAINREWIEYHVKKCDVIMPLVAIATPKLYVEDPIGVYNLDFEMNLNVV
                     KQCVKYRKRVVFPSTSEVYGACPDPEFSEDESFLVVGPIQKERWIYSCCKQLLDRVIY
                     AYGTRGHLEFTLFRPFNWIGPRLDSLYAAKEGSSRVVTQFIAELLMRRPITLVDGGRQ
                     KRCFTYVDDGIAALMKILANKDDRCRNEIINIGNPDNECSVAELAHLMKKIFMDHPDH
                     RRDGEYSEITEVAAAEYYGKGYQDIYTRKPSIAKARKLLDWEPAIDLETSLTKTIYSF
                     LEENETHPGTEDRC"
     gene            3258..4118
                     /locus_tag="PLT63_08810"
     CDS             3258..4118
                     /locus_tag="PLT63_08810"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOD98863.1"
                     /translation="MKRILGLKIDVDTHEGMKKGVPRLLESLAALEVKGTFYLSMGPD
                     ASGLAILQLLKNPRFLKKMLKSNAPALYGIKTALYGTLLPSPFIALSFPDLVRRIMAE
                     GHEVEFHAWDHRRWQDGLSRRSEGWIEDWFHKGLNAFRAVTGREAASFGAPAWLVDDR
                     VLRIAGRRGFKYLSCTRARAPFLHEMIGVPEIPSDLPCFEELGIRAAGEEIISCLKGG
                     GIHVLPVHAEVEGGAWNQPFLDLMGTIRGMDCEVRTLGEISTDLAPQGLPRRKYELAL
                     LPGRAAPCAV"
     gene            complement(4221..6062)
                     /locus_tag="PLT63_08815"
     CDS             complement(4221..6062)
                     /locus_tag="PLT63_08815"
                     /inference="COORDINATES: protein motif:HMM:TIGR03904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HOD98864.1"
                     /translation="MFLPVTPEEVRAHGWRALDVILVTGDSYVDSPYVGVAVIGKFLL
                     ASGYRVGIIPQPDLAGGADITRLGEPELFWGVTGGCIDSLVANRTAAGKRRRSDDFTA
                     GGINNRRPDRAVIAYTNLIRRYFPKTKPIVLGGIEASLRRIAHYDFWSDQVRRSILFD
                     AKADYLVYGMGENTVRDLADLLRAGRRVTDLKGICYAAGEKKDGFLAIPSWEEAATSP
                     EAFTRMFLTFYRNQDTLTARGLAQRHGGRWLIHNPPAAPLTQKEMDEVYDLDFARDAH
                     PCHRRAGAVRALETIRFSIPTHRGCYGECNFCAIAVHEGRTVSSRSEASIIREGSLIA
                     SRPDFKGYIHDIGGPTANMYGFECRKKLARGVCPDRRCMYPKICANLQPDHGPQRALL
                     RKLAALPGVKKGIVASGIRYDLLLADGKQGLPYLRDVVNRHVSGQLKVAPEHAVERVL
                     RCMGKPGIAPFLSFRDHFYRLTAQAGKQQFLSCYFIAAHPGCTQDDMEGLRRFTRREF
                     HAPADQVQIFTPTPGTISTLMYCTERNPFSGRRIFVEKTASGRERQKQILVEKKIPFR
                     YAEKNNQATVTEGKTMGKDKDVKKETKKKPSKTLKEKKEAKRQKRAG"
     gene            complement(6329..>6669)
                     /locus_tag="PLT63_08820"
     CDS             complement(6329..>6669)
                     /locus_tag="PLT63_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98865.1"
                     /translation="TPDKFPARVDFITSVGHLTGGASRREAGLLGNGPIAVVTNAGVF
                     DFEEENKRMRVQSLQPGVNFELAQLCTGFELLRPEGEIPVTPAPSPEVLAILRTQVDP
                     NGVFTSIPGM"
BASE COUNT         1179 a         1988 c         2166 g         1236 t
ORIGIN      
        1 tgagatccct ggggagaccc tatgagatcg tcatgatcga cgacggcagc cgggatggat
       61 ccctggcgat cctcaaggat cttgtccggg agaatccgga gctgcgggtg gtggaactca
      121 cccggaacta cggccagcac gccgccatta tggcgggttt ctccatcgtc cggggggaca
      181 tcgtcgttac ccttgacgcg gatctccaga atccgccgga ggagatcccc cgcctggtgg
      241 cggagatgga aaacggcggc tacgacgtcg tcgggacgat ccggcgccgg cgccgggatt
      301 cggccttccg cctcggggcc tcgcggctca tcaacatcgt ggcgcggcgc atcaccggcg
      361 tccacatgac ggactgggga tgcatgctcc gggcgtaccg ccgccccgtg gtggaacgga
      421 tgcgggaatg tcacgagcat gccaccttca tcccggccct ggcgacggta ttcgccaagc
      481 ggttgacgga gatcgaggtg gcccatgagg aacgccacgg cggcaagtcc aactaccccc
      541 tgcgcaagct tatcaacctc cagttcgacc tggtggcctc cttctccgac tttcccatga
      601 agatgctcat gtacggcggc atcctcatgt ccgtcctggg ggtctgcttc ggcgtctttc
      661 tcgccgtggc gcggctggtc tatggggccc tctgggcggc tcagggcgtc ttcacccttt
      721 tcgccgtcct cttcatcttc gtgggattgc aatttttcgc cctcggcgtc atcggcgagt
      781 atatcggccg gatctaccgg gaggtgagaa ggcgtccgga atacgtcatc gaacatatcc
      841 acggcgtccg ggaccggagc gggtcgtgaa ggccgtcgtc tttgcctatt ccaacatggg
      901 cgtaacgggc ctggaggccc tggcgcgcca cggcttcgac atccttgggg tggtcactca
      961 ccgggacgat ccggaagaaa aacaatggta tgcctcggtg gcggcgtggg cggagggaag
     1021 ggggctcccg gtctgccgtc ccgaagacgt caacgccccg gaatggacgg cgcggatcgc
     1081 cgccctggcc ccggaggtca tcttctcctt ctattaccgt aaaatgttac gcagcgagat
     1141 cctggcgctg gcccccgccg gggccttcaa cctccacggt tccctcctgc ccgcctaccg
     1201 ggggcgggtc cccgtgaact gggtactggc gaacggggag gaacggaccg gcgtcaccct
     1261 gcactggatg accgcccggg ccgacgccgg ggacatcgtc agccagcggg ccgtggcgat
     1321 agattttgac gatacggcct ttaccctgta cgggaagctg tgccgggagg ccgcgctcct
     1381 cctcgacgag acgctgcccc tgatccggtc gggacggacc ccccggatcc cccaggacca
     1441 cgcccgggcc acctgtttcg gcgggcggaa gccggcggac gggcgtatcg actggaaccg
     1501 gccggcccgg cgaatctacg acctcatccg ggccgtcgcc gatccctatc ccggggcctt
     1561 caccttcctg cccgacggga cgaagatgct nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn tcgccgatcc ctatcccggg gccttcacct tcctgcccga cgggacgaag
     1741 atgctgatct ggtggggtct ccccgaaccg gttgcgacgc cgccggcaga tgggggagcg
     1801 gcgctggaaa cggctccggc ggcggcgggg acgccgctgg ctttcccggg acaggcggca
     1861 ggcccaccat ccggagcggg gggcaacgga gagggcgtcc tttcaggggc gacccccgcc
     1921 ggcacagaag gccgggataa gaatgggagt ccaggcgatc ccccgccggg actggtcctt
     1981 gtcgatgaac ggcgggaggt gtacgtggcg accggaacgg gccgtctccg gctcctccac
     2041 gtcgagattg ccggaaaaag gatgagcgat gaggagatat ggcaatattt ccgagatctg
     2101 ggcggtgtaa tcgtcttggg ctgagccctc gcccccggga cggaaaaccg ccccgtttcg
     2161 ggacatgacc tgaaccgtat gcccgccgcc atgaggggtg cttttatttg tcaaaaaagt
     2221 caaacgtata ggagtgcatt tgcagatgaa gatattgatc ttgggcgtaa acggatttat
     2281 cggacaccat cttaccaacc gcatcctggc aacgacggat tggcgggttt acggcatgga
     2341 tttaagtgaa gaccgtctgg agagcgccct gtgcaatccc cgtttccagt ttttagaggg
     2401 cgatatcgcc atcaaccggg agtggatcga gtatcatgtc aaaaagtgcg acgtcattat
     2461 gcccctggtg gccatcgcca cgccgaagct ctacgtggag gaccccatcg gggtttacaa
     2521 cctcgacttc gagatgaatc tgaatgtcgt caaacagtgc gtcaagtacc gcaaacgggt
     2581 ggtcttccct tccacgtcgg aggtttacgg ggcctgcccc gatcccgagt tcagcgagga
     2641 cgagagtttc ctggtcgtcg gacccatcca gaaggagcgc tggatctact cctgctgcaa
     2701 gcagcttctg gaccgggtga tctacgccta cgggacccgg ggtcatctgg aattcaccct
     2761 ctttcggccc ttcaactgga tcggtccccg cctcgattcc ctctatgcgg ccaaggaggg
     2821 aagctcacgg gtggttaccc agttcatcgc cgagctcctc atgaggcggc ccatcaccct
     2881 ggtggacggg gggcgccaga agcggtgctt cacctatgtc gatgacggga tcgccgccct
     2941 gatgaaaata ttggcaaaca aggacgaccg gtgccggaac gagatcatca acatcggcaa
     3001 tccggacaac gagtgctccg tggcggaact ggcccacctg atgaaaaaga tcttcatgga
     3061 ccatcccgac caccggcggg acggggaata ttcggaaatc accgaagtgg cggcggcgga
     3121 atactacggg aagggctatc aggacatcta cacccgcaaa ccgagtatcg ccaaggcccg
     3181 gaagcttttg gactgggaac cggcgatcga cctcgaaacc tccctgacca agacgatcta
     3241 ctccttcctg gaagaaaatg aaacgcatcc tgggactgaa gatcgatgtt gacacccacg
     3301 aggggatgaa aaagggggtt ccccgcctcc tggagagcct ggcggccctg gaggtcaagg
     3361 ggaccttcta cctcagcatg ggtcccgacg cctcggggct ggcgatccta caactcctga
     3421 aaaatccccg tttcctgaaa aagatgctga aaagcaacgc cccggccctc tatggcatca
     3481 agacggccct gtatggaacg ctcctgccgt cgccgttcat cgccctgtcc tttcccgatc
     3541 tggtccgccg gatcatggcg gagggccacg aggtggagtt tcacgcctgg gaccaccggc
     3601 gctggcagga cggcctgtcc cgccgctccg aagggtggat agaggattgg ttccacaagg
     3661 gtctgaacgc atttcgcgcg gtgacgggtc gggaggccgc ttccttcggt gcccctgcct
     3721 ggcttgtgga cgaccgggtg ttgcggattg ccggccgccg gggcttcaag tacctgagct
     3781 gcacccgggc gcgggcgccc tttctccacg aaatgatcgg cgtccccgag atcccttccg
     3841 acctgccctg tttcgaggaa ctgggtattc gggcggcggg ggaggaaatt atttcgtgcc
     3901 tgaaaggggg cggcatccat gtcctccccg tccacgccga ggtggagggg ggagcctgga
     3961 accagccctt tctcgatctc atggggacaa ttcgggggat ggattgcgag gtgcggaccc
     4021 tgggggaaat atccacggac ttggctcccc aaggcctgcc caggcgaaaa tacgagttgg
     4081 ccctcctccc cggccgggcc gccccctgcg cggtgtgaca tcaatccgtc cgggaaatcg
     4141 ttccttacgg cagagaattt aagcagatca atgagatgcg cccgctgata cggcccgcgg
     4201 gaacaacggc cgcaaccgca tcaaccggct cttttctgcc gtttggcttc ctttttctcc
     4261 ttcagcgttt tggatggttt cttcttggtt tctttcttga cgtctttgtc cttgcccatg
     4321 gtttttccct ccgttacagt ggcctgattg tttttctctg cataccggaa ggggattttt
     4381 ttctcaacca gaatttgttt ttgccgctcc cggccggagg ccgttttttc cacaaagatg
     4441 cgacggccgc tgaaggggtt tcgttccgtg caatacatga gcgtggaaat agtgccgggg
     4501 gtgggggtga agatctggac ctgatcggct ggggcgtgaa actcccggcg ggtgaagcgg
     4561 cggagccctt ccatgtcgtc ctgggtgcag ccgggatggg cggcgatgaa atagcaggag
     4621 agaaattgct gcttccccgc ctgggcggtg aggcggtaaa agtggtcgcg gaaggagaga
     4681 aagggcgcga tgcccggctt ccccatacaa cggaggaccc gttccacggc gtgttccggg
     4741 gcgaccttga gttggccgga gacgtggcgg ttgaccacgt cgcgaagata ggggaggccc
     4801 tgcttcccgt cggccaggag gaggtcgtaa cggatgcccg aggcgacgat gccctttttc
     4861 acgccgggca gcgcggcgag tttccggagg agggcgcgct gggggccgtg atcgggttgg
     4921 aggttcgcgc agattttggg gtacatgcaa cggcggtccg ggcagacccc ccgggccagc
     4981 tttttcctgc attcaaatcc gtacatgttg gccgtcggcc ccccgatatc gtggatataa
     5041 cccttgaaat cgggccggga ggcgatgagg gacccttccc ggattatcga cgcctcactc
     5101 cgggaactga cggtgcgccc ctcatgaacg gcgatggcgc agaagttaca ttccccatag
     5161 caaccccggt gggtggggat ggagaagcgg atcgtctcca gggcccggac ggcaccggcc
     5221 cggcggtggc agggatgggc gtcccgcgcg aagtccaggt catagacctc gtccatttcc
     5281 ttctgggtca ggggcgccgc cggggggttg tgaatcagcc aacggccgcc atgacgttgg
     5341 gcgaggcccc gggcggtcag ggtgtcctgg tttcggtaga aggtgaggaa catcctggta
     5401 aacgcctccg gactcgtcgc cgcttcctcc caggaaggga ttgccaggaa accgtctttc
     5461 ttctctccgg cggcatagca gatacccttg aggtcggtta ccctccggcc ggcccggagg
     5521 aggtcggcaa gatcccggac cgtattttcc cccatgccgt aaacgaggta atccgccttg
     5581 gcgtcaaaca aaatggaacg gcgaacctga tcggaccaaa agtcataatg ggcgatacgc
     5641 cggaggctgg cctcgatgcc ccccaggacg atgggcttcg tcttcgggaa gtaccgccgg
     5701 atcaggttgg tataggcgat gacggcccgg tcgggacggc ggttgttgat tcccccggcg
     5761 gtgaagtcgt cggagcggcg ccttttaccg gcggcggtgc ggttggcgac aagggaatct
     5821 atgcagccgc cggtgacgcc ccagaatagt tccggctccc ccagacgggt aatgtccgcg
     5881 ccgccggcga ggtcgggctg ggggatgatg ccgacgcggt atccggaggc gaggaggaac
     5941 ttgccgatca ccgcgacgcc cacatagggg ctgtccacgt aactgtctcc cgtgaccagg
     6001 atgacgtcga gggctctcca gccgtgggcg cggacctctt ccggagttac gggcaaaaac
     6061 acggggtgtc gctttccttt cctcgcgctc gattcggacc ggtcggcgga tctccggccc
     6121 ccgtcgttcc gtcccggggg ccgagccggg gcaacggacc ggcggccact tgagagtacg
     6181 ccgtgcgccg gcctcaccgg gtgggcgccg caatctcggc ccggaactat acggggaacc
     6241 cgctgggctg tcaagccatc gtaggagggc gggaggcggc ggccgcaagg cgccgcctcc
     6301 cggaagaagg gaggatggag aatgttgttt acattccggg gatgctggta aagacgccgt
     6361 tgggatcgac ctgggtcctg aggatcgcca ggacctccgg ggacggcgcc ggggtgacgg
     6421 gtatttctcc ttccggccgc agcagttcga agccggtgca aagctgagcc agctcgaagt
     6481 tgacaccggg ctggagggat tggacccgca tgcgcttgtt ttcttcctcg aaatcgaaga
     6541 cgccggcatt ggtgacaacg gcgatggggc cgttacccag gagccccgcc tcccggcggg
     6601 aagcgccccc ggtgaggtgc cccacgctgg tgatgaaatc cacccgggcg ggaaacttat
     6661 ccggggtgt
//
LOCUS       DAOSMF010000103         6577 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_12837_length_6577_cov_19.093376, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000103 DAOSMF010000000
VERSION     DAOSMF010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 6577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6577
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12837_length_6577_cov_19.093376"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1856)
                     /locus_tag="PLT63_08825"
     CDS             complement(<1..1856)
                     /locus_tag="PLT63_08825"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD98866.1"
                     /translation="MRGRLFYKIFASYLVLVVLVMAVMEFFLTPKIRETITQNVRERM
                     IGQGRIMVLLPLEELVKKTADLAAGAQARITVIDAAGRVLADSSRQDQKWDNHLNRSE
                     VQEARIRGLGAAVRYSRTLGRNMLYIAVAVGREGEIRGYVRLACSLEDVSASIDHLYR
                     SIYLTLFIIALPSLVLAFFFSRGIAAPIRRMTEFTEKIRSGERPAPLLADGEDELGKL
                     TKDINAMLAEQEEKIRLATEEKSKLEAAFAGMVEGVLTLDRANHIDQFNRSLRKMIGR
                     RQDEIRGKTLLEVFHSAALREAFLRFRETGKPLTAEITVGDERPLALEVNISAIAGLP
                     ADEGKIMMVFHNVTRLKQLERMRMDFVANVTHEIRTPLTAIIGYVETLLQGAASQDAR
                     QREKFLQTIQANAQRLNRLVDDLLTLSNIETGEIRLHRESVAPDELIAEALKTIQPRL
                     EGKKIKSFTRIPPHIPPLWVDRDRAVQVLLNILDNAVKYTPAGGRITVSATVQTELRE
                     ELTEKGGPGVGVETKAAGRDRFTGEDEAAATRNGGPEAFVAIIIADTGPGVPKLELPR
                     LGERFYRVDKTRSRELGGTGLGLSIVKHLMAAHGGRLEIDSAPGQGTTVALF"
     gene            complement(1876..2613)
                     /gene="phoU"
                     /locus_tag="PLT63_08830"
     CDS             complement(1876..2613)
                     /gene="phoU"
                     /locus_tag="PLT63_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HOD98867.1"
                     /translation="MDTKRHTSKQYETELQEVKESLLYLGALTQQAIEDSIRALLDRD
                     TGLARKVIANDAFIDKLDGEVEEKCIRLLALRQPAARDLRFITTAIKINGHLERIGDM
                     AVKICEKVALLNEEAQLKPYIDLPRMAEIARGMIRKSLDALVKEDVALANEVRREDEA
                     VDSLNEQIFRELLTFMMEDPRTIHRSLLIMQISKTVERISDHAKGIADMVVFMITGKN
                     VRHQWPFDEEEAIRPGGRKQVKPSQIQ"
     gene            complement(2750..3523)
                     /gene="pstB"
                     /locus_tag="PLT63_08835"
     CDS             complement(2750..3523)
                     /gene="pstB"
                     /locus_tag="PLT63_08835"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HOD98868.1"
                     /translation="MARHDNGSTIIKTSGINFYYGSFQALTDITMDFDRHRVTALIGP
                     SGCGKSTLLRLLNRMNDLIDGARVGGEVLFEGMNIYAPEVDPVEVRRRIGMVFQKPNP
                     FPKSIFNNIVYGPRLAGVAAKADLEALAEQSLRQAVLWDEVKDILGRSAMTLSGGQQQ
                     RLCIARALAMKPDVLLMDEPTSALDPISTAKIEELIDELKKTYTIIIVTHNMQQAARV
                     SDTTGFFYLGRLIEYNATEKIFTNPDVKQTEDYITGRFG"
     gene            complement(3526..4392)
                     /gene="pstA"
                     /locus_tag="PLT63_08840"
     CDS             complement(3526..4392)
                     /gene="pstA"
                     /locus_tag="PLT63_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HOD98869.1"
                     /translation="MTGTMKWRYRKQKIFFGGVRLAALTLTAALGGLLFFIFINGFQA
                     ISWEFLTQPPRDSMTKGGIMPAILGTIYLTVGAIAVALPLGVMAAVYLTEYAQQGRIV
                     RAIRVGVNCLAGVPSVVFGLFGLGFFVVFLQFGSSILSGALTLGILILPTIIGASEEA
                     LKAVPQTFREASLALGVSKWLTIWKIVLPNALPGILTGSILGIGRAAGETAPIMFTAA
                     AFFTAKLPGSVFDEVMALPYHVYVLATAGTYIEQTRPLQYGTILVLVALVLGIDLIAI
                     IIRGYMRRKKRW"
     gene            complement(4404..5291)
                     /gene="pstC"
                     /locus_tag="PLT63_08845"
     CDS             complement(4404..5291)
                     /gene="pstC"
                     /locus_tag="PLT63_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HOD98870.1"
                     /translation="MTRYQKEIVIKNAFFTLSLISIMVLGLIVIFLFREGLPIFREVS
                     LRDFLFGREWYPTYDPPSYGIWPLIVASLIVTFFSSLLAVPLGVLSAVYISEIAPPAI
                     KEILKSIIELLAGLPSVVLGFFGMVTVAPWLQETFDLPTGLNIVNASAILALMAVPTI
                     SSISEDALYAVPQDFKEASYALGATKFETIVRIIVPAALSGISTAVILGMARAIGETM
                     VVLMVAGGAAAIPESIFDSVRPMPASIAAEMGEAPFRSGHYHALFATGIVLFFMTLAF
                     NLIADYLAQKFKEVGSATL"
     gene            complement(5299..6117)
                     /locus_tag="PLT63_08850"
     CDS             complement(5299..6117)
                     /locus_tag="PLT63_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HOD98871.1"
                     /translation="MSRLIKIIAVFMLCVVVAAPAALAGGNVVIKGSTTVLPVAQAAA
                     EAYMKIHKNVNISLSGGGSGDGIKALIDKSTDIANSSREIKKQEMDLAKKRGVNPVAH
                     TVAIDAIIPIVHPKNKVQNLSLDQLSQIYQGKITNWREVGGDNLTIVVVSRDSSSGTY
                     ESWGYLVLNGAKVTPRAQLQASNGAVVQAVSKNRYAIGYIGIGYLNKSVNALTVGGVQ
                     ASAKTALAKEYPIARPLYMYTNGTPKGETEGFIKFLLGKKGQEIVKKEGFVPVR"
BASE COUNT         1262 a         1812 c         2082 g         1421 t
ORIGIN      
        1 aaaaacaggg cgaccgtggt gccttggccg ggggcgctgt cgatctccag gcgcccgccg
       61 tgagccgcca tgaggtgttt gacgatggac agccccagtc ccgtgccgcc caactcccgg
      121 gagcgggtct tgtccacccg gtagaaacgt tcccccaaac gggggagttc caatttgggg
      181 acccccggcc cggtgtcggc gatgatgatg gcaacgaaag cttccggtcc gccgttcctg
      241 gttgcggccg cttcgtcctc acccgtgaac cgatcgcggc cggccgcttt ggtctcgaca
      301 ccgacccccg ggccgccttt ttccgttaat tcttccctta attctgtttg caccgtcgcc
      361 gataccgtta tccttccccc ggccggggtg tatttcaccg cattgtcgag gatgttgagg
      421 agcacctgga cggcccggtc ccgatccacc cagaggggag ggatatgggg gggaatccgg
      481 gtgaaggatt tgatcttctt tccctccagg cgaggctgga tggtcttcag ggcctcggcg
      541 atcaactcgt ccggggccac gctctcccgg tgcagtcgga tctccccggt ctcgatgttg
      601 gacagggtga ggaggtcgtc aacgaggcgg ttgaggcgct gggcattggc ctggatggtc
      661 tggaggaatt tttcccgctg ccgggcatct tgggaggcgg ccccctggag cagggtctcc
      721 acgtagccga tgatggccgt caggggcgtc cggatctcat gagtcacgtt ggctacgaag
      781 tccatgcgca tccgttcgag ctgtttgagg cgggtgacgt tgtgaaagac catcatgatc
      841 tttccctcgt cggcgggtaa gcccgcgatg gcggagatgt taacctccag tgccaggggc
      901 cgctcatctc ccacggtgat ctccgcggtc aacggcttgc ccgtttcccg gaaacgcagg
      961 aaggcctccc ggagcgcggc gctgtggaag acctccagga gggttttccc ccggatctcg
     1021 tcctggcggc ggccgatcat tttccggagg ctccgattga actggtcaat atgatttgcc
     1081 ctgtcgaggg taagcacccc ctccaccatc ccggcgaagg ccgcctcgag cttgctcttt
     1141 tcttccgtag cgaggcggat cttttcctcc tgctccgcga gcatggcgtt gatatccttg
     1201 gtcagttttc ccaattcatc ttccccgtcg gcaaggaggg gcgccggtct ttccccggaa
     1261 cggatctttt cggtgaattc cgtcattctc cgaatgggcg ccgcgatgcc ccgggagaag
     1321 aaaaatgcca gcaccaggga gggaagggcg atgatgaaga gagtaaggta gatggagcga
     1381 tagagatggt cgatggacgc ggaaacatct tccagggagc aggcgagacg gacgtagccg
     1441 cgaatttccc cttcgcggcc cacggcgacg gcgatgtaaa gcatgttccg ccccagggtg
     1501 cggctgtagc ggaccgcggc gcccagcccc ctgatcctcg cttcctggac ctcggagcgg
     1561 ttgaggtgat tgtcccactt ttggtcctgc cgggaggaat cggccaagac ccgcccggcg
     1621 gcgtcgatga cggtgatcct tgcctgagcg ccggcggcca gatccgctgt tttctttacc
     1681 agctcttcca aaggcaggag caccatgatc cgtccctggc cgatcatcct ttcccttacg
     1741 ttctgggtga tcgtttcccg gatcttcggg gtgaggaaga attccatgac cgccatgacc
     1801 aggaccacga ggaccaggta ggaagcaaat attttataga acagacgacc tctcacggcg
     1861 gcatttcacc ctaaattatt ggatttgaga aggctttact tgtttgcggc cgccaggtcg
     1921 aatggcctcc tcctcgtcaa acggccactg atggcggaca ttcttgccgg tgatcatgaa
     1981 gaccaccatg tcggcgatgc ccttggcatg atcggagatc cgctccacgg tcttggagat
     2041 ctgcatgatg aggagggaac ggtggattgt ccgggggtct tccatcatga aggtcagcag
     2101 ttcccggaag atctgttcgt tgaggctgtc caccgcctcg tcctccctcc ggacctcgtt
     2161 ggccagcgct acgtcctctt tgaccagggc gtccaggctc ttccggatca ttccccgggc
     2221 gatttccgcc atccggggca ggtcgatgta cggcttcaac tgggcttcct cattgagcaa
     2281 ggcgaccttt tcgcagatct ttaccgccat gtccccgatc cgttccaggt ggccgttgat
     2341 cttgatcgcc gtagtgatga agcggagatc ccgggccgcc ggctgccgga gggcgagcag
     2401 gcggatgcac ttttcctcca cctcgccgtc gagcttgtcg atgaaggcgt cgttggcgat
     2461 gaccttccgg gccagaccgg tatcccggtc cagaagggcc cggatgctgt cctcgatggc
     2521 ctgctgggtc agggcgccga gatagaggag actctccttc acctcctgga gctccgtttc
     2581 atactgcttg ctggtgtgcc gtttggtgtc cattgtttct ccttttaaac ccatccccac
     2641 cctaaccctc cccttgaagg ggaggcggaa aatgttctct aatcctcctc gtgaaaggga
     2701 gggggaaagg gtgtcctaat tctccccgca caaaggtgag ggatctgtct taaccgaaac
     2761 gtccggtgat gtagtcttcc gtctgtttca cgtcgggatt ggtaaagatc ttttccgtgg
     2821 cgttgtattc gatgagcctt cccaggtaga agaaacccgt ggtatcggaa acccgggccg
     2881 cctgctgcat gttgtgagtg acgatgatga tggtatatgt ttttttcagt tcgtcaatca
     2941 attcttcgat cttggcggtg gaaatggggt ccagggccga ggtgggctcg tccatgagga
     3001 ggacgtcggg tttcatggcc agggcccggg cgatgcagag gcgctgctgc tgaccccccg
     3061 agagggtcat ggccgaccgg ccgaggatgt ctttcacctc gtcccagagg acggcctggc
     3121 ggaggctttg ttccgccagg gcctccaggt cggccttggc ggcgaccccc gccaggcgcg
     3181 ggccgtaaac gatgttgttg aatatcgact tgggaaaggg attcggcttc tggaagacca
     3241 tgccgatgcg gcggcggacc tccacgggat ccacttccgg ggcgtagatg ttcatccctt
     3301 cgaagaggac ctcccctccc acccgggccc cgtcgatgag gtcgttcatc cggttgagga
     3361 ggcggagcag ggtcgatttg ccgcagcccg aaggaccgat gagggccgtc acccgatgcc
     3421 tatcgaagtc catggtgatg tccgtcagag cctgaaaact tccgtaatag aaattgattc
     3481 cgctggtctt gataatggtg ctgccgttgt cgtgccgtgc caatgttacc accttttttt
     3541 cctgcgcatg tagccgcgga tgattatggc gatgagatca atgcccagaa ccagggcgac
     3601 gaggacgagg atcgtgccgt actgaagggg tcgggtctgt tctatgtagg tcccggcggt
     3661 ggccaggaca tagacgtgat aggggagggc catgacctcg tcgaagacgg agccgggaag
     3721 cttggcggtg aaaaaggccg cggcggtgaa catgatgggg gccgtttccc cggcggcccg
     3781 gccgatgccc aggatggagc cggtgaggat gcccggcagg gcgttgggca ggacgatctt
     3841 ccagatcgtc agccacttgg acaccccgag ggccagggag gcctcccgga aggtctgggg
     3901 caccgccttg agggcctctt ccgaggcgcc gatgatcgtg gggaggatga gaatccccag
     3961 ggtgagggcg ccggaaagga tggaggaacc gaactggagg aatacgacga agaagcccag
     4021 accgaagagc ccgaagacca cggaggggac cccggcgagg cagttgacgc ccacgcggat
     4081 ggcccggacg atccttccct gttgggcgta ttccgtaaga tagacggcgg ccatgacgcc
     4141 cagggggagg gccacggcga tggcccctac ggtgagatag atggttccca ggattgccgg
     4201 catgatgccg cccttggtca tggaatcccg ggggggctgg gtgaggaact cccaacttat
     4261 cgcctgaaag ccgttgatga agatgaaaaa aagcaatccc cccagggcgg cggtgagggt
     4321 cagggcggcc aggcggacgc ccccgaagaa tattttctgt ttccggtagc gccacttcat
     4381 cgtgccggtc atggtttgcc tcgtcatagt gtcgcggacc ccacttcctt gaatttctgg
     4441 gcgaggtagt cggcgatgag attgaaggcc agggtcatga agaacagaac gatacccgtg
     4501 gcgaagaggg cgtgatagtg gccgctccgg aagggggcct cccccatctc ggcggcgatg
     4561 ctggccggca tgggccggac ggagtcgaag atgctctccg ggatggcggc ggccccgccg
     4621 gccaccatta ggaccaccat ggtttccccg atggcccggg ccatgccgag gatcaccgca
     4681 gtggagatcc ccgaaagggc ggcggggacg atgatccgga cgatggtttc gaactttgtt
     4741 gcccccaggg cataggaggc ctccttgaaa tcctggggaa cagcgtagag ggcgtcttcg
     4801 gagatgctgg agatggtggg aacggccata agggccagga tcgccgaggc gttcacgata
     4861 tttagccctg tgggcaaatc gaaggtttcc tggagccagg gggcgacggt gaccatgccg
     4921 aaaaagccca ggactacgga aggcaggccg gcgaggagtt cgatgatgga tttcaggatc
     4981 tccttgatgg ccggaggggc gatctcggag atatagacgg ccgacagcac ccccagggga
     5041 accgcgagga gggaagagaa gaaggtgacg ataagtgagg cgacaatcaa gggccagatc
     5101 ccgtaggaag ggggatcgta ggtgggatac cactccctgc cgaagagaaa gtcccggagg
     5161 gatacctccc ggaagatcgg cagcccctcc cggaagagaa agatgacaat tagtcccagg
     5221 accatgatgg agatgagcga aagggtgaag aaggcgtttt tgatgacgat ttctttttga
     5281 taacgggtca tggcttatct accggacggg aacgaagcct tccttcttca cgatttcctg
     5341 acctttcttg ccaagaagga atttgatgaa cccctctgtc tcccctttag gtgtcccgtt
     5401 ggtgtacata tacagggggc gggcgatggg atattccttg gcgagggcgg tttttgccga
     5461 ggcctggaca ccgcccacgg tcagggcgtt gacggacttg tttaggtagc cgatgccgat
     5521 gtagccgata gcgtacctgt tcttcgagac agcctgaaca acggcgccgt tggaggcctg
     5581 tagctgggcc cggggcgtca ccttggcgcc gttgaggacg aggtaccccc aggattcata
     5641 ggtccccgag ctgctgtctc gggataccac cacgatggtc aggttgtctc cgcccacctc
     5701 cctccagttg gtgatcttgc cctggtagat ctggctcaac tggtcgaggc tcagattctg
     5761 gaccttgttt ttcggatgga cgatggggat gatggcgtcg atggccacgg tgtgagctac
     5821 ggggttaacc cctcttttct ttgccaggtc catttcctgc ttcttgatct ctcgggaaga
     5881 attggcgatg tccgtggatt tgtcgatcag ggccttgatc ccgtctccgg agccgccccc
     5941 ggaaagagag atattgacgt tcttgtggat cttcatgtag gcctccgccg ccgcctgggc
     6001 taccggcagc accgtcgtgg agcctttgat gaccacattg ccgccggcca gggccgccgg
     6061 ggcggcgact accacgcaca acatgaaaac ggcgatgatc ttgatgagtc ttgacatttt
     6121 tcccctcccg attttttgtc cgattatagt cgcgaattgt tgccgtttta tgacagaaca
     6181 attaaagttc tgcaaaataa acaacctggg gaagagaggg atagccgcct ccggatgatc
     6241 cggaagcagt ttttttaccc tatggcaata aagaactccg ccatgcgggg aggtaaaatg
     6301 ccagtcgcgc aaaaaattgc gaaaggccat tttttgccgc tcatgaaatc gacttttttt
     6361 cgcaaaatcg tcaatttact aatattatca ttattttaag tgttatgaat tattcataat
     6421 tttttttggg ggtgatcgcg gatcgttaaa atagcgattt tttgggctct aaaatcgcta
     6481 ttttaagaaa attgagcggt acgaagaggt aagccgctgc gcgacccggc gaatgaccgt
     6541 ggttacctta taagcctggt aatgccttgc ggcgtaa
//
LOCUS       DAOSMF010000104         6572 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_12855_length_6572_cov_16.101580, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000104 DAOSMF010000000
VERSION     DAOSMF010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 6572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6572
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12855_length_6572_cov_16.101580"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            505..4266
                     /locus_tag="PLT63_08855"
     CDS             505..4266
                     /locus_tag="PLT63_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOD98872.1"
                     /translation="MAHRIEIGFREGIRDALGEKIKRRINEHLHLPVDEVRTVEVYTL
                     DGEFSREELERAATGPLSDAVIQHYAIDGAVAAARAAAVGMTTNIEPPATARGTTAGA
                     TGADAAATAGAGARADGANAASGTEANAEPPAAAAAWDFDWLIEVGFRPGVTDNVGKT
                     AREAIAILLGLPAAARPFRVHTSRQYLLRGALSRAEAERVASQLLANELIERYEVIAR
                     ADWDPVRGLAPYVPRVTGTERPATELIDLDVTDDALLAISNERVLALTLEEMQILRDY
                     FRDPAVQAERARAGLPAAARITDAELECLAQTWSEHCKHKIFNSRIAYDDGTGRITEI
                     DSLFKTYIKGSTERIRAERRARAAQAVRIAGDAPAAATTAEPSAGADTGAAGDFCLSV
                     FVDNAGVIRFNDDYSLVFKVETHNSPSALDPYGGALTGIVGVNRDPFGTGMGAKLIFN
                     TDVFCFAPPDYDRPLPKRILHPRRIYEGVREGVEHGGNKSGIPTVNGCLVFDERYLGK
                     PLVYCGTGGIMPARLHGKPTHTKEIVPGDLIVMTGGRIGKDGIHGATFSSEELHEGSP
                     VTAVQIGDPITQKKMTDFLLKARDLGLYRCITDNGAGGLSSSVGETAQLSGGCDLDLR
                     RAPLKYPGLNPWEILISESQERMTLAVAPEQRDAFLALAAKMDVEATVLGTYTDTGMF
                     HVRYGNETVAYLGMDFLHDGLPQMRLRARWERPRRPEPDLPVPAPSPAAMAVAGEAAA
                     AAATATVSGQAPASPGSATAPGTAPRPEAAPADMGAALRRMLSRLNICSKESVVRQYD
                     HEVQGGSVIKPLTGAANDGPSDAAVIRPLLDTFAGIVVANGICPRYSDIDAYDMAACA
                     IDEAVRNAVAVGADLDHLAGLDNFCWCDPVASEKTPDGEYKLAQLVRANQAVYDYTTA
                     YGVPCISGKDSMKNDYAIEGTKISIPPTLLFSVIGRMADVRRAVTMDAKRAGDIVYVL
                     GETKNELGASEWYALHGCVGDNCPRVDAAAAKWLYAALHRAIAAGLVASCHDCSDGGL
                     GIALAETAFAGGLGMDVDLRQVPRRDLHRDDYLLFSETASRFVVTVSPANRAAFETEL
                     YVGRQTPRPENRQSISGLRPDELTENSSGMEIGDSVPLSSTTGASGKNQEIAPTAAMI
                     AAATSTGESGQLSSSMANNGAASLSSSPPSPSHSGTGKIGANAEETSTGMAPVPAFAE
                     ESPEPPPVVFAAVGVVTAAPLLTIRGLGGDIILREPLAELKAAWQQPLP"
     gene            4573..5661
                     /locus_tag="PLT63_08860"
     CDS             4573..5661
                     /locus_tag="PLT63_08860"
                     /inference="COORDINATES: protein motif:HMM:NF024897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HOD98873.1"
                     /translation="MSKIVKSIVITGNGTNCEVEMAHACRLAGADVVDIVHISELLAG
                     EKRLDDYDFLNLPGGFLDGDDLGAAKAGANRIRHAAIAGTEEKLYDQMTRFIRDGKLI
                     LGVCNGFQLLVKLGLLPGCDGDYQTQTATLTYNDSGRFEDRWVHLTVNPASPCIFTRG
                     LSHLYLPVRHGEGKFIPQDEAIRARLHREGHVALQYCNQTGGQVFKISVFPSVDIHGQ
                     TKKGGTTATTPAATFSAGIDSPTGSASTFGTEAIHAPTFTGSTAFGAASGSFQTSRVA
                     ATSAAPAAPEAPGASRPTMSYPDNPNGSVDAIAGICNATGRIFGLMPHPEAFLHRVNH
                     PRWTREDLPDAGQGLALFQNAVAFLRTA"
     gene            complement(5766..6089)
                     /locus_tag="PLT63_08865"
     CDS             complement(5766..6089)
                     /locus_tag="PLT63_08865"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOD98874.1"
                     /translation="MNKGYSIEWTVVAEGDLRRIIDHIAIDHPGNARQIPSEIREKAS
                     NLYDFPERGRIVPELRTQGIDTYRELIVSPWRIVYRISGRSIYVLSVFDGRRNIEDVL
                     LDRLL"
     gene            complement(6086..6379)
                     /locus_tag="PLT63_08870"
     CDS             complement(6086..6379)
                     /locus_tag="PLT63_08870"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HOD98875.1"
                     /translation="MNIINDIKPVTYLKARAADLLDQINETHRPVIITQNGEPRAVLQ
                     DPESYENMRNTIGILKLLSQGEADIRDGRTKPQEDVFAEIESALNASMNNIPE"
BASE COUNT         1268 a         2269 c         1877 g         1158 t
ORIGIN      
        1 ttgatcttgg aactgactgc ttttttcatg aattgacctc cacctactcc catatagtat
       61 ttcagtccgt caaggccctg atataagcat agatgctgcc gccggcctta aaaagcccgc
      121 ttccggccac ggtgccacgg cgcgactccc cccgcggcgg cgccgggcga caatacccgg
      181 ccgcggcggc gcctctatat gcaataagtg ccataaagtc aaccgtaaag gcgcgccggc
      241 cgtggcaccg catgtcgcag tgacgaagga aaaagcgcaa caccgtatcc ggactgctaa
      301 gtaagtcgca cgtaactaat tggattcttt agatacgtgt ttgacggttt tgggccggtt
      361 tcatggcaca gactcgactt acttctttta tacaggcccg gcagtcggcg ggctagggcg
      421 ctttttactt cgtcgtcatc tttgcttgac aggccaccgc gctttaccta tactcggcgc
      481 caaaaatcga gtcgcgagag aaagatggcc caccgcatcg aaatcggctt ccgggaaggc
      541 atccgggacg ccctgggcga aaaaatcaaa cgccgtatca acgagcatct ccacctcccc
      601 gtggatgagg tgcggaccgt ggaggtttac accctggacg gcgaattctc ccgggaggag
      661 ctggaaaggg cggccaccgg tcccctgtcc gacgccgtga tccagcacta cgccatcgac
      721 ggcgccgtgg ccgccgccag ggcggcggcc gtggggatga cgaccaatat agaacccccg
      781 gcgacggcga gagggacgac agccggggcg accggcgctg atgccgctgc gaccgcggga
      841 gccggggcga gggctgacgg ggcgaatgct gcatccggga cagaggccaa cgcagaaccc
      901 ccggcggcag cggcagcctg ggatttcgac tggctcatcg aggtaggctt ccggcccggc
      961 gtcacggaca acgtgggcaa gaccgcccgg gaggccatcg ccatccttct gggcctaccc
     1021 gccgccgccc gccccttccg ggtccacacc tcccggcagt atctcctccg gggcgccctc
     1081 agccgcgccg aggcggagcg cgtcgcctcc cagctcctcg ccaatgagct catcgaacgc
     1141 tacgaggtga tcgcccgggc cgattgggac cccgtccggg gcctcgcccc ctatgtcccc
     1201 cgggtcaccg gcacggagcg ccccgccacg gagctgatcg atctcgacgt cactgacgac
     1261 gccctcctgg ccatcagcaa cgagcgggtc ctcgccctga ccctggagga gatgcagatc
     1321 cttcgggatt acttccgcga ccccgccgtc caggcggagc gcgcccgggc cggcctaccc
     1381 gccgccgccc ggatcaccga cgcggagctg gaatgcctgg cccagacctg gtccgagcac
     1441 tgcaagcaca agatcttcaa cagccgcatc gcctacgacg acgggaccgg ccgcatcacc
     1501 gagatcgact ccctcttcaa gacctacatc aagggttcga cagagcgaat ccgcgccgaa
     1561 cgccgcgccc gggccgctca ggctgtccgg atcgcaggtg acgccccggc cgccgccaca
     1621 accgcagaac catctgccgg cgcagatacc ggtgccgccg gtgatttctg cctctccgtc
     1681 ttcgtggaca acgccggcgt catccgcttc aacgacgact acagcctcgt cttcaaggtg
     1741 gaaacccaca actccccctc cgccctggat ccctacggcg gcgccctcac cggcatcgtg
     1801 ggcgtcaacc gcgacccctt cggcacgggc atgggtgcca agctgatctt caacaccgac
     1861 gtcttctgct tcgccccgcc ggattacgac cgccccctcc cgaagcggat cctccatccc
     1921 cggcggatct acgagggtgt ccgcgagggc gtggagcacg gcggcaacaa gagcggcatc
     1981 cccaccgtca acggctgcct ggtcttcgac gagcgctacc tcggcaagcc cctggtctat
     2041 tgcggcaccg gcggcatcat gcccgcccgc ctccacggca agcccaccca caccaaagaa
     2101 atcgtccccg gcgatctcat cgtcatgacc ggggggcgca tcggcaagga cggcatccac
     2161 ggcgccacct tctcctccga ggagttgcac gaaggctcgc ccgtcaccgc cgtccagatc
     2221 ggcgacccca tcacccagaa gaagatgacg gatttcctcc tcaaggcccg ggatctgggg
     2281 ctctaccggt gcatcaccga caacggcgcc ggcggtctct cctcctccgt gggcgagacg
     2341 gcgcaactct ccggcggctg cgatctggac ctccggcggg cccccctgaa gtaccccggc
     2401 ctgaatccct gggagatcct catctccgaa tcccaggagc ggatgaccct cgccgtcgcc
     2461 cccgagcagc gcgacgcctt cctggccctg gccgccaaga tggacgtcga ggccacggtc
     2521 ctggggacct acacggatac ggggatgttc cacgtccgct acggcaacga gaccgtcgcc
     2581 tatctgggca tggatttcct ccacgacggc ctgccccaga tgcggctccg ggcccggtgg
     2641 gagcggcccc gccgccccga acccgacctg cccgtgccgg ccccgtcgcc ggcggcaatg
     2701 gcagttgcgg gagaagccgc ggcagcggcg gctaccgcaa ccgtatcagg gcaggcgccg
     2761 gcatcgccag gatcggcaac agcccccggc accgcgccca gacctgaagc cgcccccgcc
     2821 gacatgggcg ccgccctccg gcggatgctc tcccggctca acatctgcag caaggaatcc
     2881 gtcgtccgcc agtacgatca cgaggtccag gggggcagcg tcatcaagcc cctcaccggc
     2941 gccgccaacg acggccccag cgacgccgcc gtcatccgcc ccctcctgga caccttcgcg
     3001 ggcatcgtcg tggccaacgg catctgcccc cgctacagcg acatcgacgc ctacgacatg
     3061 gcggcctgcg ccatcgacga ggcggtccgg aacgccgtcg ccgtcggggc ggatctcgac
     3121 catctcgccg gcctggacaa cttctgctgg tgcgatcccg tcgcctcgga gaagaccccc
     3181 gacggggaat acaagctcgc ccagctcgtc cgggccaacc aggccgttta cgattacacc
     3241 acggcctacg gcgtcccctg catctccggg aaggacagca tgaagaacga ctacgccatc
     3301 gaggggacga agatctccat cccaccgacg ctgctcttct ccgtgatcgg ccggatggcg
     3361 gacgtccgtc gggcggtgac catggacgcc aaacgggccg gggacatcgt ctatgtgctg
     3421 ggggaaacga aaaacgaact gggggcctcg gagtggtacg ccctccacgg ctgcgtgggc
     3481 gacaactgcc cccgggtgga cgcggcggcg gcaaaatggc tgtacgccgc cctgcaccgg
     3541 gccatcgcgg cgggcctcgt ggcctcatgc cacgactgct ccgacggcgg gctggggatc
     3601 gccctggcgg agacggcctt cgccggcggg ctgggcatgg acgtggatct gcggcaggtc
     3661 ccccgccggg acctccaccg ggacgactac ctcctcttct ccgagacggc cagccgcttc
     3721 gtcgtcaccg tctccccggc caaccgcgcc gccttcgaga cggaattata cgtgggcaga
     3781 cagacccccc gtcccgaaaa caggcaatct atttcaggtt tacgccccga tgagttgaca
     3841 gaaaactcct ctggaatgga aattggagac agcgtccctc tttcttcaac cacgggggca
     3901 agtggaaaaa atcaggaaat cgcaccaacg gcagctatga ttgcagcggc aacatccaca
     3961 ggggaatcgg gacaattgtc atcttcaatg gcaaataatg gagcagcgtc gctatcatcg
     4021 tcgccgccgt ccccctcgca ttccgggacc ggaaaaatcg gcgcaaatgc agaagaaaca
     4081 tcaacgggca tggcgccagt gccggctttc gcggaggagt ctcccgaacc gccaccggtg
     4141 gtcttcgccg cggtgggggt cgtgacggcc gcgccgttgc tcaccatccg gggcctgggc
     4201 ggcgacatca tcctccggga gcccctggcg gaattgaagg cagcctggca gcagccgctg
     4261 ccctgagaat gaaacatgat ggcctgctca gaagcgtttt tcttcatccc ccttactaag
     4321 gaggaggagt ttaggagggg catggggtaa gaggatgtaa tatcaacggg caggcttaaa
     4381 cccatcctca ccatagccct ccccttgaag gggaggggac aactatattc tgggcctccc
     4441 cttgaagggg agggagcaat aagataaata aggggaagtg cccacatatt ttatatgaaa
     4501 atagttccaa cagattgaat attttcatct ttcgttgtgc ccgcgccggg catgggggtt
     4561 agaaggtatt gaatgagcaa gatcgtcaaa agcatcgtca tcacgggaaa cggcacgaac
     4621 tgcgaggtgg agatggccca cgcctgccgc ctcgccgggg ccgacgtcgt ggacatcgtc
     4681 cacatcagcg aactccttgc cggggagaag cgcctcgacg actacgattt cctcaatctt
     4741 cccggtggtt ttcttgatgg cgacgacctc ggcgccgcca aagccggggc gaaccggatc
     4801 cgtcacgccg ctatcgccgg cacggaggaa aagctctacg atcagatgac ccgcttcatc
     4861 cgggacggca agctcatcct gggggtctgc aacggcttcc agctcctcgt gaagctgggg
     4921 ctcctcccgg gctgcgacgg cgattaccag acccagaccg ccaccctgac gtacaacgat
     4981 tccggccgtt tcgaagaccg ctgggtccat ctaaccgtca atcccgcctc gccgtgcatc
     5041 ttcacccggg ggctttcgca cctctacctc cccgtgcgcc acggcgaagg gaagttcatc
     5101 ccccaggacg aggccatcag ggcccgcctc caccgggagg gccacgtcgc cctccagtac
     5161 tgcaaccaaa ccgggggtca ggtctttaaa attagcgttt tcccttccgt ggatatccac
     5221 ggccaaacaa aaaaaggcgg cacaaccgcc accactcccg ccgccacttt ctccgccggc
     5281 attgattccc ccaccggcag cgcctcgacc ttcggcacag aggcgatcca cgccccgacc
     5341 ttcaccgggt caactgcctt tggcgccgca tctggcagct tccaaacctc ccgtgttgcc
     5401 gccacgtccg ccgctcccgc cgcccccgag gcccccggcg cctcccgccc caccatgtcc
     5461 tacccggaca accccaacgg ctccgtggac gccatcgccg gcatctgcaa cgccaccggc
     5521 cggatcttcg gcctcatgcc ccacccggag gccttcctcc accgtgtcaa ccatccccgc
     5581 tggacccgcg aagacctccc cgacgccggc cagggcctcg ccctcttcca aaacgccgtc
     5641 gccttcctcc gcacggcctg acccaaaaag cgccaagttt ctaattcgat atcttgatcg
     5701 gaagcagcgt ggccttactc aattttgaaa aaccgccaag atgagtccgt cagacggtta
     5761 ccctgttaca gcaatctatc caggaggacg tcctcgatat tccgtctgcc gtcgaaaacc
     5821 gacagaacat agattgaacg tcctgaaatc cgatagacta tacgccaggg tgatacgatc
     5881 aattctctgt aggtgtctat tccctgtgtc cgaagttcgg gaacgatccg gccccgttct
     5941 ggaaagtcgt agagattgga tgctttctct ctgatttcac tgggtatctg cctggcatta
     6001 ccgggatggt caatagcgat atggtcgatt attcgccgta aatcaccttc ggcaaccacc
     6061 gtccattcaa tcgagtatcc cttattcatt ctgggatgtt attcatgctg gcattcagtg
     6121 ccgactcgat ttccgcaaac acatcttcct gtggtttggt ccggccgtcg cgaatatccg
     6181 cctctccctg ggaaaggagc ttcaatatcc cgatggtgtt gcgcatgttt tcatagctct
     6241 cgggatcttg cagtaccgcc cggggctccc cgttctgggt aattattacc gggcgatgag
     6301 tttcgtttat ctgatccagc aaatccgccg ctctggcctt cagataggta actggtttga
     6361 tatcgttgat gatattcatg gtattaacct caggttcgta attggtacca tattcaaacc
     6421 gcttgtcaag aaatatcaca cggaaattac ccagggcacg ccgcctaatt cttgctctaa
     6481 ttcctgctct aattcctgcc gatgaggttg agggcttggg aggcggcgat tttgaccttc
     6541 ttgtggtagg tagtcaccgt gaagaagccg gg
//
LOCUS       DAOSMF010000105         6534 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_12942_length_6534_cov_25.911406, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000105 DAOSMF010000000
VERSION     DAOSMF010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 6534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6534
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12942_length_6534_cov_25.911406"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="PLT63_08875"
     CDS             complement(<1..407)
                     /locus_tag="PLT63_08875"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOD98876.1"
                     /translation="MSYHILANCSGCGACARICPTEAIAGEKKSLHVIDPRRCLECGA
                     CGKICPVAAVRDAFGIVCRRVKRSAWEKPRLDEKLCMSCGICIDACPANCLDWSEPVM
                     KKEPHAYPLLREEKLCIGCGFCARECPVGAIAMT"
     gene            complement(433..2361)
                     /locus_tag="PLT63_08880"
     CDS             complement(433..2361)
                     /locus_tag="PLT63_08880"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HOD98877.1"
                     /translation="MSGGYLGRILWVDLVAGACREEEVADEIYGQFLSGIGLAVRLLG
                     DRIPPGADPLGPENVLAFVSGLLTGTGALFSGRWMVAGKSPLTGGWGDANCGGTLSPA
                     IKRCGYDGIFFTGVSPRPVYLLVDNGRPELRDASHLWGKDALAAEEELIREAGGHARA
                     AVIGPAGEKLSLIAGICNDRGRIAARSGLGAVMGAKRLKGLVLKGKRRIPVHHREEVQ
                     RLSRECNKWVQFQPPFVTGTMTAYVGTLMRLLPTQMAMDGLVYKLLLKKWGTVSMNQM
                     SVEMGDAPIMNWKGTSADWGIGKSRTVNPDAFRVLERVKYHCYSCPLGCGGICATKGP
                     FGEVHKPEYESVLALGGLCMNSDLESIITMNELLNRAGMDTISAGGTVAFAMECYEQG
                     ILSRDDLDGLDLRWGNSAAMIALIEKMIRREGVGDLLADGAKRAAEKIGRGAGACAIH
                     AGGQELPMHDGRNDPGFNVHYSVEATPGRHTIGAQLYYEMFRLWKRIPSLPRPPLFYR
                     KRRKYLADEEKAVMAAACSKFMNVANGAGLCMFGLFLGAERIPSFAWLNAAAGWRKSP
                     EEYLAIGAAIQNRKQEFNRRQGIDARSLQVPDRVLGRPPQRRGANRGRTMDLEKMRSD
                     YWEQFGWDREKGLPKDRP"
     gene            complement(2381..3628)
                     /locus_tag="PLT63_08885"
     CDS             complement(2381..3628)
                     /locus_tag="PLT63_08885"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOD98878.1"
                     /translation="MYLPGYYEYSCRVKIVAGHDALEGIPALLRHVGARRPLLVTDKG
                     VAGAGLVDIVRQAIGDRLEIGAVADDVPPDSDLKVVHRLSALYRENGCDAMIAVGGGS
                     VMDTAKGINIVVSERAEDLMKFSGSGALKRPLKPLIAVPTTAGTGSEVTLVAVIKDHE
                     RHLKMAFVSFFLLPDAAILDSRMTLSLPAPITASTGMDALTHAVEAYYCLSKNPLSDV
                     TALAAIRLISGNLLKVVRKPSDREGRLAMANAATLAGMAFSNSMVGMVHTLGHATGSV
                     CGVPHGVCMAILLPYGLEYNRHKREVVMGELLFPIGGPGIYGQTAPEDRTNRVIAYVR
                     QLNEDLHEMTGGRHARFLGEVIDREGNPAVPRERLPDIARTALGDGSIFYNPEDLDYD
                     DLLMVLEAAWEGMPLDSGRIRKG"
     gene            complement(3704..4873)
                     /locus_tag="PLT63_08890"
     CDS             complement(3704..4873)
                     /locus_tag="PLT63_08890"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOD98879.1"
                     /translation="MFQSAHTFVFCSPAKVLSGNRALENLPVELAGMDACRPLILTTA
                     GLAVRGMERIMAGAFADSQLTVGFFTGVPPCPDRELVRALYGLYRDRGYDSLIALGGG
                     PVLTVAKALNIAVSGEPEDLEAAAAGTLESKELRPLTAIPNLDVSGYEMTRYADLASL
                     SLTAPTAIPGLLVLDHRTVPAEDRKTTAAAALLTLTQAVEALLHPGRNHLTDAYAYGA
                     ARFIMENFRTALKNPSERGSRLALANAAFFAQTAFDNLSGGKIFCLGMAISRRCAVSP
                     GLVMGLLLPHGLLMEMDREETPGKDLFLAVGGAEDYALVEEGMGLRKTVDRCVELLLE
                     LQSLIPDYPRSIRDIGLKKSDLEELPGAADEVTGRGTLALPEVRYLLDKAWETRF"
     gene            complement(4885..5433)
                     /locus_tag="PLT63_08895"
     CDS             complement(4885..5433)
                     /locus_tag="PLT63_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98880.1"
                     /translation="MESLTMDNFREIPAGGKPFKRIYVALMLWFVGRAIQAAARVDEA
                     VGEEFAALPDGFTFSLGVLPRGPAMIVGKGEEGQVRYRGWRLAGQRIDLQMKIKNLEG
                     ALLMFTFQESTAVATARNRLIVDGEVPAACAIVRILDRVEVYLLPKIIAKLAVKRYPD
                     WPWEEKVGNRIRIYAGAILGFR"
     gene            complement(5592..6425)
                     /gene="truA"
                     /locus_tag="PLT63_08900"
     CDS             complement(5592..6425)
                     /gene="truA"
                     /locus_tag="PLT63_08900"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOD98881.1"
                     /translation="MTVTKFFKYQLTLEYDGSGYRGWQIQENARSVQGTLLTAATELF
                     GAEVDIQGAGRTDAGVHALGQVAHLTVKKALPPPRIKAGLNDLLPAGLNILRVEQVPG
                     AWHARHDAVSRSYIYIVSRTRTAFGKRYVWWVKDPLDAEKMKEACRLFAGFHDFASFV
                     DRRLERNVSTQVQIDAAEIEERGELIIFRVTGSHFLWKMVRRMVGVLVEAGRGNLTTK
                     EVAALLASRSDLPARLTAPPSGLYLAQVLYPGDTAAPLTLPPPLMLAGNGKQATGERE
                     R"
BASE COUNT         1231 a         2187 c         1913 g         1203 t
ORIGIN      
        1 gtcatcgcga tggccccgac ggggcattcc cgggcgcaga agccgcaacc tatgcagagc
       61 ttttcctctc tgagcagggg ataggcatgc ggttccttct tcattaccgg ctccgaccag
      121 tccaggcagt tcgccgggca ggcgtcgata cagatgccgc aggacatgca gagcttctcg
      181 tccaaccggg gcttttccca ggcggaacgc ttgacgcggc ggcagacgat gccgaaggca
      241 tcccgcaccg ccgccaccgg gcagatcttt ccacaggccc cgcactcgag gcaccggcgg
      301 ggatcgatga catggagaga cttcttttcc ccggcaatgg cctccgtggg gcaaatacgg
      361 gcacaggccc cgcaaccgct gcaatttgcc aatatgtgat aggacatgat actgccttct
      421 cgatgggtct ccctacggcc tgtctttggg cagccccttt tcccgatccc agccgaactg
      481 ctcccaataa tcgctccgca tcttttccag atccatggtc cggccccggt tggccccccg
      541 tctctgggga ggtcgaccga gcacccggtc gggaacctgc aaggaacggg catcgatacc
      601 ctgccgacga ttgaattcct gcttccggtt ctggatggcc gccccgatgg ccaggtactc
      661 ctcggggctc ttccgccatc ccgccgcggc attgagccac gcaaaggacg gaatccgctc
      721 cgcccccaga aagagcccga acatgcaaag accagcgcca ttggccacgt tcatgaactt
      781 gctgcacgcc gcggccatga cggccttttc ctcatcggcc aggtatttgc gccgcttgcg
      841 gtaaaacagc ggcggccgcg gcagggaggg tatccgcttc cagagccgga acatctcgta
      901 gtagagctgc gccccgatgg tgtgacggcc cggcgtggcc tccacggagt aatggacgtt
      961 gaaaccggga tcgttgcggc cgtcatgcat gggcaactcc tgccccccgg cgtggatggc
     1021 gcatgccccg gcgccccggc cgatcttttc cgccgccctt ttggcgccgt ccgccagcaa
     1081 atcgcccacc ccctcccggc ggatcatctt ttcgatcagg gcgatcatgg cggcggagtt
     1141 gccccaccgc aggtccaggc cgtccagatc atcccggctc aggatcccct gttcgtaaca
     1201 ctccatggcg aaggccaccg tcccccccgc cgagatggtg tccatccccg cccggttgag
     1261 caattcgttc atggtgatga tgctctccag gtcactgttc atacagagcc cccccaaggc
     1321 caggacggat tcatactccg gcttgtggac ctctccgaaa ggccccttgg tggcacaaat
     1381 gccgccgcag cccaacggac aggagtagca gtggtacttt accctttcca ggacccggaa
     1441 ggcgtcggga ttgacggtcc gtgatttacc gatcccccag tccgcgcttg tccccttcca
     1501 gttcatgatc ggggcgtcgc ccatctccac ggacatctgg ttcatgctca ccgtccccca
     1561 tttcttcagg aggagtttgt aaaccagtcc gtccatggcc atctgggtgg gcaggaggcg
     1621 catcagggtc cccacgtagg ccgtcatcgt tccggtaacg aagggcggct gaaactgcac
     1681 ccacttgttg cactccctgc tcagacgctg gacctcttcc cggtgatgga cgggaatgcg
     1741 ccgtttgccc ttgaggacca ggcccttgag gcgcttggcg cccatgacgg cccccagccc
     1801 ggaacgggcg gcgatgcggc cccggtcatt gcagatcccc gcgatgaggg aaagcttctc
     1861 cccggccggg ccgatgacgg ccgcccgggc atgccccccc gcttcccgga tcaattcctc
     1921 ctccgccgcg agcgcgtcct ttccccacag atgggaagcg tcccgcaact ccgggcggcc
     1981 gttatcgaca aggagataga ccggacgagg gctgacgccg gtgaaaaaga tcccgtcgta
     2041 gccgcaacgt tttatggccg gagacagggt gccgccgcaa ttggcatccc cccagccgcc
     2101 ggtcaggggt gatttccccg cgaccatcca gcggccgctg aagagcgcgc ccgtgccggt
     2161 gaggagtccg gaaacgaacg cgaggacatt ctccggcccc agaggatcgg ctccgggagg
     2221 aatgcggtcg ccaagaagcc ggaccgccag gccgattccc gagaggaatt gcccgtagat
     2281 ctcatcggcg acttcttcct cgcgacaggc gccggcaaca aggtccaccc acaggatccg
     2341 tcccagataa ccgccgctca tgagttcacc ctctttccca tcatcccttc cggatccttc
     2401 ccgaatccag gggcatccct tcccaggccg cctccaggac catgagcaga tcgtcatagt
     2461 ccagatcttc cggattgtag aagatggagc cgtcacccag ggccgtgcgg gcaatgtccg
     2521 gaagccgctc ccggggcacc gcgggatttc cctcccggtc aatgacctcg ccgagaaaac
     2581 gggcatgccg tcctcccgtc atctcgtgga ggtcttcgtt gagctggcgg acataggcga
     2641 taacccggtt tgttcggtcc tctggagcgg tttgcccgta aatgccggga ccgccgatgg
     2701 ggaaaagaag ctcccccatg acaacctccc gcttatggcg gttgtattcc agaccgtagg
     2761 gaagcaggat ggccatgcac accccgtggg ggaccccgca gacggaacca gtggcatgcc
     2821 ccagggtatg caccatcccc accatggaat tggaaaaggc catgcccgcc agggtggcgg
     2881 cgttggccat agccaggcgc ccttcccgat ctgacggctt ccggacgacc ttgaggagat
     2941 ttccactgat gagccggatg gccgccaggg cggttacatc actcagggga ttcttactca
     3001 gacagtagta ggcctccacg gcgtgggtca gggcatccat cccggtagac gccgtgatcg
     3061 gggcaggcag actcagggtc atgcgggaat cgagaatggc cgcgtcggga aggaggaaga
     3121 aactcacgaa cgccatcttc agatgccgct cgtgatcctt gattacggca accagggtga
     3181 cctcggaacc ggtgcccgcc gtggtcggca cggcaattag gggcttcagg ggccgcttca
     3241 gcgctcccga cccgctgaat ttcatgagat cttccgcccg ctcggaaacc acgatattga
     3301 tccccttggc cgtatccatc acggaacctc cgcccaccgc gatcatggcg tcgcagccgt
     3361 tttcccggta aagggcggac aggcggtgca ccaccttgag atcggaatcg gggggaacat
     3421 catcggcgac ggcaccgatc tccagtcgat ctcctatcgc ctgccggaca atatccacga
     3481 gccccgctcc ggcgactccc ttgtcggtaa ccagcagagg ccggcgggcg ccgacgtggc
     3541 gaagcagggc ggggatcccc tccagggcgt catgccccgc gacgatcttc acccggcagg
     3601 aatattcata atatccaggt aggtacatct ctttgctctc ctcgatccgt cttcctctcg
     3661 ctcccccggc cgggatgtcc gccgcaacag gtcatcccgc cggtcagaat ctcgtttccc
     3721 aggccttgtc cagaaggtaa cggacctcgg gcagggcaag ggttccccgg ccggtaacct
     3781 catctgcggc tccgggaagt tcttccagat cacttttctt aaggccgata tcccggatgc
     3841 tccggggata atcgggaatg aggctctgaa gttccagtag cagctccaca cagcggtcca
     3901 ccgtcttgcg gaggcccatc ccttcctcga ccagggcata atcctccgcc ccccccactg
     3961 cgaggaaaag gtcctttccc ggcgtttcct cccggtccat ttccatcaga agtccatgag
     4021 gaagaaggag ccccatgacc agacccgggc tgaccgcgca gcgccgggag atggccatac
     4081 cgaggcaaaa gatctttccc ccggacaggt tgtcgaaggc cgtctgggcg aaaaaggccg
     4141 cattggccaa ggcgagtctg ctcccacgct cagatgggtt cttcagggcc gtccggaagt
     4201 tctccatgat aaatctggcc gccccatagg cgtaagcatc cgtcaagtgg ttgcggcccg
     4261 gatggaggag ggcctccacg gcctgggtca aggtaaggag agcggcggcg gcggttgttt
     4321 tacggtcttc cgctgggacc gtgcgatggt cgagaacgag cagcccggga atggctgtcg
     4381 gcgccgttag ggacagtgag gcgagatcgg cgtaacgggt catttcgtag ccggaaacat
     4441 cgaggttcgg aatcgccgtc aggggccgca gctctttgct ctccagcgtg ccggcggccg
     4501 cggcctccag atcttccggc tctcccgata cggcgatatt gagggccttg gccactgtca
     4561 gtaccggtcc gcctccgagg gcgatcaggg aatcgtagcc cctgtcacga tacagcccgt
     4621 acaaagcccg gacaagttcc cgatcggggc aagggggaac gccggtaaaa aagccgacgg
     4681 ttagttgaga gtcggcgaac gccccggcca tgatccgctc catcccccgg accgccagtc
     4741 cggccgtggt gaggatcagc ggccggcagg catccatacc cgccagttcc accggcaggt
     4801 tttccagggc cctgttaccg gaaagcacct tggcgggact gcaaaaaacg aaagtatgcg
     4861 cggattgaaa caccgtcctc ctccttatcg gaatcccagg atggcgccgg cgtaaattcg
     4921 gatgcggttg ccgacctttt cctcccaggg ccagtcggga taacgcttca cagccagttt
     4981 cgcgatgatc ttcggcagga gataaacctc cactcggtcg agaatccgga cgatggcgca
     5041 ggcggcggga acctcaccat caacgatgag gcggttcctg gccgtggcca ccgccgtgct
     5101 ctcctgaaag gtaaacatca ggagcgcccc ttccagattc ttgatcttca tctgcaggtc
     5161 tatcctttgc cctgcgagcc gccagccccg gtagcgtact tggccttcct cgcctttgcc
     5221 cacgatcatc gccggccccc ggggcaaaac ccctagggaa aaggtgaatc catccggcag
     5281 ggcggcgaat tcctccccga ccgcctcatc cacccgggcc gccgcctgaa tggcccggcc
     5341 gacaaaccag agcatcaggg ctacatagat ccgtttgaag ggtttgccgc cggccgggat
     5401 ctcccggaag ttatccatag tcaacgattc cataaccgca atgatgacct ccccttcgtt
     5461 tccgttcgga caatcctcct cttttgcatc gccgtcgata aaccatcccg cccctccaaa
     5521 tcgatggccg ggatcgccac taatcccggg cgaaaagcag agggcaagaa acaccccgcc
     5581 gcgcatccgt cttaccgttc cctctccccc gttgcctgtt taccgtttcc ggccagcatc
     5641 aatggcggcg gtagggtaag cggcgcggcc gtatctcccg gatagagcac ttgggctagg
     5701 tagagcccgg acggcggggc ggtaagccgg gccggcagat cggaccggga agcgagcagg
     5761 gccgccactt cctttgtggt caggttcccc cgtccggcct ccaccaggac cccgaccatg
     5821 cgccggacca tcttccagag aaaatgggaa cccgtcaccc ggaagataat cagttcaccc
     5881 ctttcctcga tctctgcggc gtcgatctgc acctgcgtcg aaacgttccg ctccaggcgg
     5941 cggtcgacaa aggaggcaaa atcgtgaaag ccagcaaaca gccgacaggc ttctttcatc
     6001 ttctccgcat ccagcgggtc tttcacccac cagacatagc gtttgccgaa ggcggtccgg
     6061 gtccgggaaa cgatgtagat gtagctcctg cttaccgcat cgtgccgggc gtgccatgcg
     6121 ccgggcacct gctcgacccg gaggatgttc agcccggcgg ggagaaggtc gttgagaccc
     6181 gctttgatcc tcgggggcgg cagcgccttt ttcaccgtga gatgggccac ctgtcccagg
     6241 gcgtgcaccc cggcatccgt cctccccgcc ccctggatat ccacctcggc gccgaacagc
     6301 tccgtagcgg ccgtaaggag cgttccttga accgaacggg cattttcctg gatctgccag
     6361 ccccgataac ccgaaccgtc gtactccagg gtcaattggt acttaaagaa ctttgtcacg
     6421 gtcatgcggc gaaccttcgg caaatacacc cggcgcagcc ggatgatgcc ggaactattt
     6481 tttcagctcc ggcacgggtt tattcctgga gacgacgata tcccgaaaac cgtg
//
LOCUS       DAOSMF010000106         6441 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_13170_length_6441_cov_15.998747, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000106 DAOSMF010000000
VERSION     DAOSMF010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 6441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6441
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13170_length_6441_cov_15.998747"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="PLT63_08905"
     CDS             complement(<1..316)
                     /locus_tag="PLT63_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98882.1"
                     /translation="MAAIILQAVEDIEGKNNATWRDIVSAVYFIRNEDPFEYYCVELG
                     LDPDAVREKVRDKIPLAIERADRLHAAAREKAEKERLARIEERRFIPPEIHSKLQEFL
                     PEP"
     gene            complement(339..1271)
                     /locus_tag="PLT63_08910"
     CDS             complement(339..1271)
                     /locus_tag="PLT63_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98883.1"
                     /translation="MEVTGCRIVKYGKRDAEFSLYDMADLHLGTRGCAKDHIRRDIER
                     IRQDTYSMFFVGGDYADWIMPGDPRFDPEAYSVDITVNDLSKIAAILTDEIVGLFSPV
                     RQKCLGFLLGNHELKHMSRNSEMFVHEDICKRLRVPNMRYSGWCDVYFVHEPGFRGCR
                     ISYSAVPPEDYMSRLRVLVHHGVGAANTAGGKLNKLKNLVDSVEADLVMMGHVHEQFA
                     KSFTRVFPNEDCSKIKSKVTMGLITGSYLRIYGPGYTSYGEQRGYFPTSLGATRARFC
                     PRTRALIVENRADNIGLAGPQLKRTENERKAVNE"
     gene            complement(1271..1489)
                     /locus_tag="PLT63_08915"
     CDS             complement(1271..1489)
                     /locus_tag="PLT63_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98884.1"
                     /translation="MARIYWTDEELMILRDAAEKGVTAEQLAQILKSRTKASIDNKAR
                     ELGVSLAGGKPEIDRKAYRRIMKELGES"
     gene            complement(1694..2578)
                     /locus_tag="PLT63_08920"
     CDS             complement(1694..2578)
                     /locus_tag="PLT63_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98885.1"
                     /translation="MAGRYQKQTVDYFPHYVDHGRVLFIMESTWGALGYAAFYKLLEV
                     LGRSEGHYFFAGNYEGREYLAARMGVTDEVCQEMMCKLAKLDVLDRELWQKAKVIWMQ
                     SFVDSLDALYRKRVIPAPEMPSIDLFLPPEIGKEAPDSGNSGTGNKQRKVKESKEKKD
                     PASLFDRFWSAYPKKKSRGDALKAFQKINPDEELLVIMVAKVEAEKATKQWKEDNGRF
                     IPHPATWLNGRRWEDEGVEVQPAGGNNGSGPAPLPVKSVVCPNCGKDTDEIVDGKCIY
                     CIERKSVKHFIDGIGKPV"
     gene            complement(2592..2879)
                     /locus_tag="PLT63_08925"
     CDS             complement(2592..2879)
                     /locus_tag="PLT63_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98886.1"
                     /translation="MNRTEAQYESFLEGLKMQGAVVDYRFEAVKLRLADKTFYTPDFL
                     VVHDDRFEFHEVKGFWREDARIKTKVAAATYPWFKFIAVTRKNKQWSFEYF"
     gene            complement(3067..4122)
                     /locus_tag="PLT63_08930"
     CDS             complement(3067..4122)
                     /locus_tag="PLT63_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007784978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOD98887.1"
                     /translation="MQITVKAKLIPTSEQREHIKTATVEYIRLINTIVSECIEAEEHI
                     KHTSGTVSATLPSALKNQAIQDAKTVYAKYRKTKTKSILKKPVCIWNNQNWTLKDGIL
                     RFPVLVNGKSTRINVPVLLSTYQLEKLNGKLGTLRITEKSGKWIAQIAVTIEDTESTD
                     AGVLGVDLGLKCPAVAVTETGRTKFFGNGRENKYMKRKFRSKRKKLGKAKKPQAIKKL
                     DDKEQRWMKDKDHKTSRQIVDFAIQEGVGTIRLEQLSGIRQTARTSRKNEKNLHTWSF
                     YRLAQYIEYKAGILGIKVEYVDPKYTSQTCPECAERNKARDRKYKCSCGFKTHRDRVG
                     AMNIIKAPVIDGNSLSA"
     gene            complement(4127..4378)
                     /locus_tag="PLT63_08935"
     CDS             complement(4127..4378)
                     /locus_tag="PLT63_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOD98888.1"
                     /translation="MTLKEWKLKRGFRTLDIAVLLGITDSGVARIVAGNRYPSPTLAR
                     KIIAVSKGEITWEDLYGKELLVVKPAGGKVKKKKRRAVR"
     gene            complement(4499..5764)
                     /locus_tag="PLT63_08940"
     CDS             complement(4499..5764)
                     /locus_tag="PLT63_08940"
                     /inference="COORDINATES: protein motif:HMM:NF040570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOD98889.1"
                     /translation="MKTQCLPDLISLDFPIDQEYHRLMQKARRIIHRAYKFRLKATPE
                     QTRQFSQIAGVCRLVWNLCLEQRHLAYTVARKSLSSFSQLPEVTALRKDYDWIESVPS
                     QVLQQKVRDLDAAYKNFFERRSDFPERKKKGKSVDSFRFPQGFKIDNRRVFMPKIGWV
                     GFFKSQEIAGTPKHMTVSRNGKHWFVSICCEVEQGIPTPKSKAVGIDMGIAKLCAMSD
                     GTVIENPKILLKYQKKLTKLQRRLAKKVKFSKNFYKAKAKITALHTKIANTRRDYTHK
                     TTTEIAKNHGLVVIEDLRVKNMSASAKGSIHNPGKNVRQKSGLNRSILDAAWGEFRRQ
                     LEYKLDWAGGILIAVDPKNTSRKCPVCGHTAKENRRTQSDFVCVACGFADDADVNAAG
                     NILRAGHARLACEVSGAVIPPAAGTLKAA"
     gene            5718..6131
                     /gene="tnpA"
                     /locus_tag="PLT63_08945"
     CDS             5718..6131
                     /gene="tnpA"
                     /locus_tag="PLT63_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HOD98890.1"
                     /translation="MGKSSEIRSGRHCVFNLHVHLVFVTKYRKRVFEKDHYVTMETIF
                     KKVCKDFDAELIEVDGESDHVHLLISYPPKVSVSKLVNSLKGVSARLLKKHHPHLCPW
                     YWKDVLWSPSYFAASCGGAPISIIRQYIEQQETPD"
BASE COUNT         1375 a         1779 c         1538 g         1749 t
ORIGIN      
        1 ttggttcagg taaaaactct tgcaacttcg agtgaatttc aggagggatg aacctccttt
       61 cctcgattcg cgcgagacgt tccttctccg ccttttccct cgccgccgca tggagccggt
      121 cggccctctc aatggcaagg gggatcttgt ccctcacctt ctccctgacc gcatccggat
      181 caaggccgag ctcgacgcag taatactcga agggatcctc gttcctgatg aaatagacag
      241 cggaaacaat atcgcgccac gtggcattgt tcttcccctc gatgtcctcg acggcctgga
      301 ggataatcgc cgccacaaga ttcctttgcg cctgtggatc actcattgac tgccttcctt
      361 tcgttttcgg ttcgtttgag ttgcggtccc gcgagaccaa tattgtcggc ccggttctcg
      421 acgatgagag cccgggtcct cggacagaaa cgcgccctgg tcgccccgag actcgtgggg
      481 aagtatcccc tctgctcccc gtaggaagtg tagccgggac catagatccg caagtaggac
      541 ccggtgatga gccccatggt gaccttcgat ttgatcttgc tgcagtcctc gttcgggaac
      601 acccgggtga aggacttcgc gaattgctca tggacgtggc ccatcatgac caggtcggcc
      661 tcgacggaat cgaccaggtt tttcaatttg ttcaatttcc cgccggccgt gttcgccgcg
      721 ccgacaccgt gatggaccag gacccggagc ctcgacatgt aatcctccgg agggacggca
      781 gagtaagaga tccggcaccc gcggaatccc ggctcatgga cgaaatagac gtcgcaccat
      841 ccggaatagc gcatattcgg gacccggagc ctcttgcaga tgtcctcatg gacaaacatc
      901 tcggagttcc tgctcatgtg tttcagctcg tgatttccca ggagaaaacc gaggcacttc
      961 tgcctgacgg gggaaaagag gccgacgatc tcgtccgtga gaatcgccgc gatcttcgaa
     1021 aggtcgttga ccgtgatgtc cacggagtag gcctccgggt cgaagcgcgg atccccgggc
     1081 atgatccagt cggcataatc gccaccgacg aaaaacatcg aataggtatc ctgcctgatc
     1141 cgttcgatgt cccgccggat gtgatccttc gcgcatcccc tcgtccccag gtgaaggtcg
     1201 gccatgtcat agagagaaaa ttcagcgtcc cgtttcccgt atttcacgat cctgcatccg
     1261 gtgacttcca tcagctctca cccagctcct tcatgatccg cctgtaagcc ttccggtcta
     1321 tttccggttt cccgcccgca agcgaaacac cgagctcccg cgccttgttg tcgatcgagg
     1381 ccttcgtcct ggatttcagg atctgcgcga gctgttccgc cgtgaccccc ttctccgcgg
     1441 cgtccctcag gatcatgagc tcctcgtcgg tccagtatat cctcgccatt cagccctccc
     1501 gggagatccg gcgcgcaacc cggacaacga gacagaccaa cgcgagggcg atgaacgccg
     1561 caaggcagta aaccgctgtc atcctatctc ctgaaagtga tcgcgtatgc cgtgatggca
     1621 aaaagaatca tgatgaacag aacctcatga ctcggcatcg ccttgatcgt ctggagaatt
     1681 tccgtcatca gggtcatacc ggtttcccta tcccatcgat gaagtgcttg accgatttcc
     1741 tttcaatgca gtagatacat ttcccgtcga cgatctcgtc cgtgtccttc ccgcaattcg
     1801 ggcagacaac cgatttcacc ggaaggggcg ccggccccga gccgttgttc ccgcccgccg
     1861 gctgcacctc gaccccctcg tcctcccacc gtctgccgtt gagccaggtc gcgggatggg
     1921 ggatgaaacg cccgttgtcc tctttccact gtttggtcgc cttctccgcc tcaaccttcg
     1981 cgaccatgat caccaggagc tcctcgtccg gattgatctt ctgaaaagcc ttcagggcgt
     2041 cgcctctcga tttctttttg gggtatgccg accagaaccg gtcgaacagg cttgcgggat
     2101 ctttcttttc tttactttcc ttgactttcc tctgtttgtt tccggtgccg gaattgccgc
     2161 tatcgggggc ttccttacct atttccggcg gaagaaatag gtcaatagac ggcatttccg
     2221 gcgccgggat tacccttttt cggtaaagcg catcgaggct gtccacaaaa ctctgcatcc
     2281 agatgacctt cgccttctgc cacagctcgc gatccaggac atccagcttc gcgagtttgc
     2341 acatcatctc ctggcagacc tcgtcggtga cacccattct cgccgccagg tattcgcgcc
     2401 cctcgtaatt accggcaaag aagtaatggc cctccgatct gcccaggacc tcgaggagct
     2461 tgtaaaaggc cgcgtatccc agggcccccc acgtgctctc catgataaaa aggaccctcc
     2521 cgtgatccac gtagtgaggg aaataatcga ccgtctgctt ctggtatctg cccgccatag
     2581 tcctgccccc tttaaaaata ttcgaatgac cactgtttgt ttttccgggt gaccgcgatg
     2641 aatttgaacc agggatatgt cgccgccgcg acctttgtct tgatccgcgc atcctcccgc
     2701 cagaaacctt tgacttcgtg aaactcgaag cggtcatcat ggacaaccag gaaatcgggc
     2761 gtgtagaaag tcttgtcggc aagcctcagc ttcacggcct cgaaccggta atccacgaca
     2821 gccccctgca tcttcaggcc ctcgaggaaa ctctcgtatt gagcctcggt cctgttcatg
     2881 acccccttct tgaagtggat gggcctcatc gcgcctctcc atgacgtaaa tgaaagaagt
     2941 tgacactccc acagctaaag caatgggatt cttgggtgat taaacgccag tctgtttcca
     3001 gtagacgagt acgaccccaa gctaagggca tgccattgcc cctcctagag cagtgcatat
     3061 agcactctag gctgataggc tgttaccatc tatcacaggt gctttgatga tattcattgc
     3121 accaactcgg tctcgatgtg ttttgaatcc acatgagcat ttatattttc tatcccttgc
     3181 cttgtttctt tctgcacact ctgggcaggt ttgactggta tatttggggt ctacgtactc
     3241 tacttttatg cctaaaatac ctgctttata ctcgatatac tgggctaagc gatagaatga
     3301 ccatgtgtgc aaattctttt cgtttttacg gcttgtcctt gccgtctggc gtataccaga
     3361 tagttgctct aggcgtattg ttccaacgcc ttcttgaatg gcaaaatcaa cgatttgacg
     3421 acttgttttg tggtctttat ctttcatcca tcgttgttct ttatcatcca atttcttgat
     3481 tgcttgcggt ttctttgctt tgccaagttt cttgcgtttg cttctaaact tgcgtttcat
     3541 gtacttgttt tctcgaccat taccaaaaaa cttggttctg cctgtttctg ttactgcaac
     3601 tgctggacat ttaagtccta aatcaacacc taacacacct gcatctgtgc tttctgtatc
     3661 ctctatagtt acagctattt gagcaatcca tttgcctgat ttctctgtga ttctaagcgt
     3721 tccaagcttg ccatttagtt tttctaattg gtaggttgat agcaacacag gcacattaat
     3781 gcgtgtactt ttgccattaa caagtactgg aaaacgcaaa ataccatctt tcaatgtcca
     3841 gttttgatta ttccatatgc aaacaggctt cttcagaatg cttttggtct ttgtttttcg
     3901 gtactttgcg tagactgttt ttgcatcttg tattgcttga ttcttcaatg cactcggaag
     3961 tgtagccgaa actgtgccac ttgtgtgttt aatgtgttct tcggcttcaa tacattcaga
     4021 gacaatggtg tttatcaaac gaatatattc gactgttgct gtttttatat gctctcgttg
     4081 ctctgatgtt ggaattaact tagcttttac agttatttgc acagtttcac cggacggccc
     4141 ttctcttttt cttcttgact ttgcccccgg ccggcttgac gacgaggagc tccttgccgt
     4201 agaggtcctc ccaggtgatc tcgcctttgg agacggcgat gatcttcctg gccagggtgg
     4261 gactggggta tctgttcccc gccacaatac gggcgacgcc gctgtctgtt atgccgagca
     4321 aaactgcaat atcgagggtc ctgaaaccgc gcttcaactt ccattcttta agggtcatgg
     4381 acggatatta taacaggaac gactatttgt caacgataaa tttttgatct gcgcgccctt
     4441 ccttctgacc tcgtcttgac atcctccccg ccctgaagga cggggattcc caaaacagct
     4501 aagctgcttt gagggttcct gctgctggcg gtattactgc gccgctcact tcacaggcta
     4561 accgggcgtg ccccgccctt aaaatatttc ccgccgcatt gacatccgca tcatcggcaa
     4621 aaccgcaggc cacacagacg aaatcagact gtgtgcgcct gttttctttt gccgtatggc
     4681 cgcataccgg gcatttccgg gatgtgttct tcggatcgac agcaatcagt atcccaccgg
     4741 cccagtccag cttgtattca agttgccggc ggaactctcc ccatgcggca tccaggatac
     4801 tccggttcaa cccggacttc tgccggacgt tcttgccggg gttgtgaatg ctgccctttg
     4861 ccgatgccga catattttta actcgcaaat cctcgatgac aaccaggccg tggtttttgg
     4921 caatctcggt tgtcgtcttg tgggtgtaat ccctgcgggt attggcaatc ttcgtgtgca
     4981 gcgcggtgat tttcgctttc gctttataga agttcttcga gaatttaacc ttcttggcca
     5041 gtcgccgttg cagcttggtc agtttcttct gatattttag tagaatcttc gggttttcaa
     5101 tgaccgtccc atcagacatg gcgcacagtt tggcgatccc catgtcaatc ccgacagcct
     5161 tgcttttggg agtagggatt ccctgctcga cttcgcaaca gatggagaca aaccagtgct
     5221 tgccattgcg ggaaacggtc atgtgcttcg gcgttccggc aatctcctga ctcttgaaaa
     5281 atccgaccca gccaattttc ggcataaaca cccgcctgtt gtcgatctta aaaccttggg
     5341 ggaaccggaa ggaatcaaca gatttgcctt ttttcttccg ttcgggaaaa tcggaccgcc
     5401 tctcgaagaa gtttttgtag gcggcgtcca gatcgcgaac cttctgctga agcacctggg
     5461 acggaacgga ttcaatccag tcgtagtctt ttctcaaggc agtgacttcc ggcaactggg
     5521 agaatgaact taaggatttg cgggcaacgg tgtaggcaag gtgccgctgt tcaaggcaca
     5581 aattccagac aagacggcac accccggcga tttgggagaa ttgccgggtt tgttccggcg
     5641 tggcttttaa ccggaattta tatgccctat gaattatgcg ccttgccttt tgcatgagcc
     5701 tatgatactc ttggtctatg ggaaagtcaa gcgaaataag atcaggtaga cattgtgttt
     5761 tcaatctcca tgtgcatttg gtctttgtga caaaataccg gaaacgggtt ttcgagaaag
     5821 accactacgt gacgatggaa acgattttca aaaaagtctg caaagatttt gatgccgaat
     5881 tgatcgaggt ggacggcgaa tctgaccatg tccatttatt aatctcatac ccacctaaag
     5941 tatcggtatc taaactcgtc aacagcctca agggtgtctc agcgagatta ctaaaaaaac
     6001 atcatccgca tttatgtccc tggtattgga aggacgtcct atggtctccg agttattttg
     6061 ctgcaagttg tggcggtgct ccaatttcaa ttattcgcca gtacatcgag cagcaagaga
     6121 ctccagattg aagaaaagga acgcctttca tcccctacct gaaggaaggg gtcttcacgg
     6181 cttggctaga taaacgaatc aggtccgagc ttgccgcgtt gaagaaggaa tcagcggata
     6241 catcacattc aaaaccggca aaaaggaggg cgcaatgact atgatctttt ctttacttta
     6301 ctttacttta ctttacttta ctttacttta tctatttccg gcactgcagg acggaggaaa
     6361 taccctctgc gacctatttc cggcgccgga aataggtatg gtaactaatt tcgggcgggg
     6421 aaatacccca atcaaaggag g
//
LOCUS       DAOSMF010000107         6387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_13320_length_6387_cov_21.174037, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000107 DAOSMF010000000
VERSION     DAOSMF010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 6387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6387
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13320_length_6387_cov_21.174037"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1520)
                     /locus_tag="PLT63_08950"
     CDS             complement(<1..1520)
                     /locus_tag="PLT63_08950"
                     /inference="COORDINATES: protein motif:HMM:NF028259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98891.1"
                     /translation="MNGVLTLLKPRLWSFRNGSLSRGAWDNRARLYFFSFIGLGFWLG
                     IFMIFYRVLTYFKGVEGFGDILAFKLMSMVVMTFFVLLIFSGVIVTLSKLYISRDLPL
                     VHSLPVTPGEIFLARWLESLIDSSWMVALFSLPIFISYGLVFKAGSAFYLLALPAVAF
                     LCVVASGLSSLAVIGAATILPAGRIRTLAVFFGVVLILFVVVAVRLMRPEQLMNPETF
                     QSVAFYLQSLETPGSPLMPTTWTYDGIRAALAGRWGEAAFHLSLSFTGGLSLLMVNLM
                     AAEKWYFRGYSRAQTAPSRLVAVPNPGRGWSGILSFLPPPVQALAVKEIKSFFRDQTQ
                     WPQLFLLLALIVVYLYNFSVLPLDRTPIRTVYLQNLFAFLNMGLAGFVLTAIAARFVY
                     PAVSMEGQAFWIIRSSPVTLRTFLMVKFFIYYIPLVFLSEVLIVVSNLLLEVTPFMMT
                     LSVITVFFMTPGIVALGVGFGAAYPDFTSENPTQSVTSFGGLLYMTICAGFIALIIV"
     gene            complement(1517..2251)
                     /locus_tag="PLT63_08955"
     CDS             complement(1517..2251)
                     /locus_tag="PLT63_08955"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD98892.1"
                     /translation="MIELKELTKYYDRTLAVKALNLSIPKGEIFGFIGPNGAGKTTTI
                     RMMGGILAPSSGAVLIDGQSLAEHPVAVKRKIGFIPDRPFLYEKLTGWEFMRFSADLF
                     GMDGGGFVGRAEELLGSFALFNWRHELIESYSHGMKQRLIIASALLHDPAVLIVDEPM
                     VGLDPGAIRMVKDIFRTLAYRGTAIFMSTHTLAVAENLCDRIGVIHRGDLIALGTMAE
                     LKASTRLREGDLEEVFLRLTEEETGS"
     gene            complement(2248..3753)
                     /locus_tag="PLT63_08960"
     CDS             complement(2248..3753)
                     /locus_tag="PLT63_08960"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOD98893.1"
                     /translation="MAADRVFRWRVIGGATILVFMILLAVRMGIIGLPGGGLGREGSR
                     ATGKTAPTTPPVEREVWMNIYQQGQKIGYAHRSAGPGGEGFRIQEEIFMQVRTMGVVQ
                     GIALRTENTLNKELALATFSFDLKSNLFAFHARGRVAGDRLLIDTRTAGEERRYEVPL
                     KEPVRLSQGMFTGVAAARLAPGQEITIPIFDPMTMGSRPVKLTYVGEEMKTAQGKTIP
                     LRKYAIDFMGARQYAWLDKEGDVVREEGMLGLALEKTSREDARSGVSGGAADLTDLAS
                     VAAGRKLEDPEGLQSLTVKLANVDVRPLALSGDRQCYDSGGLLTVRKERRGGLNPGRS
                     GDLENRDVYMAATPFIQSSHPDVRFRVGEIVAGSAGDEEKARRIVAWVHGNIEKRPVL
                     SVANAVETLKNRAGDCTEHAVLTAALARAAGVPAVMEAGLVYQRGRFYYHAWNAFWLP
                     AWGGWVTADAALNQFPADVTHLRLVRGEAERQLDLMGIVGRIKLEIKDMSR"
     assembly_gap    3875..3974
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3975..4802
                     /locus_tag="PLT63_08965"
     CDS             <3975..4802
                     /locus_tag="PLT63_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HOD98894.1"
                     /translation="GLIGKKIVATLTSTGTQAIFLHPVEGMHGDLGIVTKRDVVLAIS
                     NSGETHELNVIVASVREIGAALIALTGNTASTLARSCDLVIDVGVEREACPFGLAPTS
                     SSTAALAMGDALAVALIEKRKFNEADFYKFHPGGNLGARLRARVREVMIAGGQTPKVL
                     TGATVAQAIEEMDINNKGFVLITDRRSILRGIFTDGDLRRLVKRGRIDTDGPIDAIMT
                     RTPKTIDENASLAETIEAMQKDEITTLVVVNGRKKLQGYIHLHDILGRGGTLKIAFP"
     gene            4974..5420
                     /locus_tag="PLT63_08970"
     CDS             4974..5420
                     /locus_tag="PLT63_08970"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOD98895.1"
                     /translation="MRVRVSAAFEQAGEILCMKYVYGGKDVYALPGGGVDRDVPLQEA
                     ITSEWKNELGVKVEVGDIVLVGEAPGTKRHPQTLHIIFAATAVHGIPKFRPDHTHSLD
                     IAWVPVAKLNECPLYPDVGKQLFAYFAEGQKRSLTFIENCMERGFW"
     gene            complement(5518..5592)
                     /locus_tag="PLT63_08975"
     tRNA            complement(5518..5592)
                     /locus_tag="PLT63_08975"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5558..5560),aa:Val,seq:cac)
     gene            complement(5882..>6387)
                     /locus_tag="PLT63_08980"
     CDS             complement(5882..>6387)
                     /locus_tag="PLT63_08980"
                     /inference="COORDINATES: protein motif:HMM:NF024934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOD98896.1"
                     /translation="TVPTALGDEIAVAVMKDGEFFGDMALLDGMPRSASASAGEETQL
                     AILERGDFLKFLAKRDHAIQAILMALSWRLRKTDDLISEICFSYLSQRLAKRIIDLVG
                     DDSGEGRQGSETQINMTQKELSEMLGVSRESVNKELKVLRDKGIVRTKRGKIIVSDLE
                     HLKKRAR"
BASE COUNT         1342 a         1979 c         1847 g         1119 t
ORIGIN      
        1 acgatgatca gggcgatgaa gccggcacag atcgtcatgt agaggagtcc gccgaaactt
       61 gtaacggact gggtgggatt ctccgaggtg aaatcaggat aggccgcccc gaaaccgacc
      121 cccagggcca cgatccccgg cgtcatgaaa aagacggtga taacggacag ggtcatcatg
      181 aacggcgtca cctccaaaag gagattcgag accacgatga gcacctcgct caggaacacc
      241 aggggaatat aatagatgaa gaatttcacc atcagaaagg tccgcagggt aacgggcgag
      301 gagcggatga tccagaaggc ctgaccctcc atgctcaccg ccggatagac gaagcgggcc
      361 gctatggccg tcaggacaaa gcccgccagc cccatgttga ggaaggcaaa gagattctgg
      421 agatagacgg tcctgatggg ggtccggtcc aggggaagga cggaaaaatt atagagatag
      481 acgacgatca gggccaaaag gagaaacaac tggggccact gggtctggtc gcggaaaaag
      541 ctcttgatct ccttcaccgc cagggcctgc accggcggcg ggagaaatga caggatcccg
      601 ctccagcccc ggccggggtt cggaacggca accaggcgcg aaggcgccgt ctgggctcgg
      661 gaatagcccc ggaagtacca tttttccgcc gccatcaggt tcaccatgag gagcgacagc
      721 ccgcccgtga aggacaaaga gagatgaaag gcagcctcgc cccagcggcc ggcgagggcc
      781 gcccggatac catcgtaggt ccaggtggtg ggcatcaggg gggaaccggg cgtctccaag
      841 gattggagat aaaaggccac ggactggaag gtttcggggt tcatcaactg ctccggccgc
      901 atgagtcgca cggcgacgac gacgaacagg atcaagacga cgccgaaaaa gacggctagg
      961 gttcggatcc gccccgccgg caggatggtc gccgccccga ttacggccag ggaactcaat
     1021 cccgaggcca ctacacagag gaacgcaacc gccggcagcg ccaggagata gaaggcggac
     1081 cccgccttga aaaccaggcc ataggaaatg aagatgggca ggctgaacag cgccaccatc
     1141 catgagctgt cgataagact ctccagccag cgggcgagaa atatctcccc cggggtgacg
     1201 ggaagggaat gcacgagggg gaggtcgcgg ctgatgtaga gcttcgacag ggtgacgatc
     1261 acgccgctga agatcagcag cacaaagaag gtcatgacga ccatggacat cagcttgaag
     1321 gcgaggatgt cgccgaagcc ctcgaccccc ttgaaatagg tgagaacccg gtagaagatc
     1381 atgaagatcc ccaaccagaa tcccaacccg atgaagctga agaagtacag ccgggcccgg
     1441 ttgtcccagg ccccccggga gaggctgccg ttccggaagg accagagacg cggtttcagc
     1501 agcgtgagga cgccgttcat gatcccgtct cctcttcggt caggcgcaga aacacctcct
     1561 ccagatcccc ttcccggagg cgggtggacg ccttcagctc cgccatggtc cccaaggcga
     1621 tgaggtcgcc gcggtggatg acgccgatgc ggtcgcagag gttttccgcc accgccaggg
     1681 tgtgggtgga catgaagatc gcggtccccc ggtaggccag ggtgcggaag atgtccttga
     1741 ccatgcggat ggctccaggg tcgagtccca ccatgggctc atcgacgatg agcaccgccg
     1801 gatcgtggag cagagcggaa gcgatgatga ggcgctgttt catcccgtgg gaataagatt
     1861 cgatcaactc gtggcgccag ttgaagaggg cgaagctgcc cagcagttct tccgcccggc
     1921 caacgaaccc gccgccatcc atcccgaaca ggtcggcgga aaagcgcatg aattcccaac
     1981 ccgtgagttt ttcatagaga aacggccggt cggggataaa accgatcttc ctcttcaccg
     2041 ccacgggatg ctccgccagc gactgcccgt cgatgagcac cgccccggaa gaaggggcca
     2101 ggatgccccc catcatgcgg atcgtcgtcg tttttcccgc cccgttggga ccgatgaagc
     2161 cgaagatctc tcctttgggg atagaaaggt tcagggcctt gacggccagg gtgcgatcgt
     2221 aatatttcgt cagctcttta agttcgatca tcgcgacatg tccttgattt ccagttttat
     2281 ccgccccacg atgcccatga gatccagttg cctttccgcc tcgccccgaa ccagacgcag
     2341 gtgggtcaca tcagcgggaa actggttcaa cgcggcatcg gcggtgaccc agccgcccca
     2401 ggcgggaagc cagaacgcgt tccaggcatg atagtaaaac cggccccgct gataaacgag
     2461 ccccgcttcc atgacggccg gaacacccgc cgcccgggcc agggccgcgg tcaggacggc
     2521 atgctccgta cagtcaccgg cgcggttttt cagggtttcc accgcattgg ctacggagag
     2581 cacggggcgc ttctcgatat tcccgtggac ccaggcaacg atccggcgcg ctttttcctc
     2641 gtcaccggcg ctgccggcga caatctcccc gacccggaag cggacgtcgg gatgggaaga
     2701 ctggatgaag ggcgtcgccg ccatgtacac atcccggttc tccagatccc cggagcggcc
     2761 ggggttcaga ccgccgcgcc gttctttgcg aacggtgagg aggcccccgg agtcataaca
     2821 ctgacggtcc ccggaaagcg ccaggggacg gacatcgaca ttcgccagct ttacggtcag
     2881 ggattgcaga ccctccggat cctccagctt ccgaccggcg gcaacagagg ccagatcggt
     2941 caaatcggcg gcaccgccgc tcaccccgga acgtgcgtcc tcccggctgg tcttttccag
     3001 ggcgaggccc aacatcccct cctcccgcac gacatcacct tctttatcga gccaggcgta
     3061 ctgccgggcg cccataaaat cgatggcata tttccgcagg ggtatggtct tcccctgagc
     3121 agttttcatt tcctcgccga cataggtcag ctttaccggc cgcgatccca tggtcatggg
     3181 atcgaagatc gggatcgtta tttcctgtcc cggggcaagg cgggccgcgg cgacgccggt
     3241 gaacatcccc tgggaaagac ggacgggctc tttcaggggc acctcgtaac gccgttcctc
     3301 ccccgccgtt cgggtgtcga tcagcaggcg gtctcccgcg acccgtcccc gggcatggaa
     3361 ggcgaagaga ttcgacttca gatcgaaaga aaaggtggcg agggccagct ctttatttag
     3421 ggtgttctcg gtccgcaggg caatgccttg gacgaccccc atcgtccgca cctgcatgaa
     3481 gatctcttcc tggatgcgaa atccctcgcc gccggggccg gcgctccggt gggcataacc
     3541 gattttttgt ccctgctggt agatattcat ccagacctcc cgctccacgg gaggggtcgt
     3601 cggggccgtc tttccggtcg ctcgcgaccc ctccctgccc agcccacccc cgggaaggcc
     3661 gatgatcccc atccgcacgg ccaacaggat cataaatacc aggatcgtcg ccccgccgat
     3721 tacgcgccac cggaaaactc tatccgccgc cattgttcct cctcttttcg ggaagtataa
     3781 ataccgccgg ggcccctgtc aacgaaaagc ccggcgccgg tggaaaaacg tccttgtcag
     3841 cggcaatatt ttctgatagc gtccctctcc cgacnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnngggctc atcggaaaga agatcgtcgc caccctcacc agcacgggga
     4021 cccaggccat cttcctccat cccgtggagg gcatgcacgg cgatctgggc atcgtgacaa
     4081 agcgtgacgt cgtcctcgcc atttccaaca gcggcgaaac tcacgaactc aacgtcatcg
     4141 tcgccagcgt ccgggagatc ggcgccgccc tcatcgccct gacgggcaat accgcctcca
     4201 ccctggcccg ctcctgcgat ctcgtcatcg acgtgggggt ggaaagggag gcgtgcccct
     4261 tcggactggc gccaacgtcc agttccaccg ccgccttggc catgggtgac gccctggcgg
     4321 tggccctcat cgaaaagcgg aaattcaacg aagcagattt ctacaagttt catcccgggg
     4381 gcaacctcgg cgcccgcctc cgggctcggg tccgggaggt gatgatagcc ggcggccaaa
     4441 cgccgaaagt cctcaccggc gccaccgtcg cccaggccat cgaggagatg gacatcaaca
     4501 acaagggctt cgtcctcatc acagaccgca ggagcatcct ccggggcatc ttcaccgacg
     4561 gcgacctccg ccgcctggtc aaacggggcc gcatcgatac cgacggcccc atcgacgcca
     4621 tcatgacccg aacccccaag accatcgacg agaacgcctc cctcgccgag accatcgagg
     4681 ccatgcagaa ggacgaaatc accaccttgg tggtcgtcaa cggccggaaa aaactccagg
     4741 gctacatcca tctacatgac atcctggggc gcggtggtac cctgaagatc gccttcccct
     4801 gaatcccctc cggatcgata tgggcacgcc ctgaactgtc cttgcggaaa gatcgtttgc
     4861 attccgtcct tcatcccggt acaatgtcca taacggcgcg acttgttcca gccgcggcgc
     4921 cgagccggcg gtgatggtct ccccggctta aaaggacaaa gaaaggagcg cttatgagag
     4981 ttcgggtatc agccgccttc gaacaggccg gagagatcct ttgcatgaag tatgtttacg
     5041 gaggcaagga cgtatatgcc ctgccggggg gcggggtgga cagagacgtg ccgctccagg
     5101 aggccataac cagcgagtgg aagaacgaac tgggggtcaa ggtggaggtg ggcgatattg
     5161 ttctcgtcgg ggaagcgccc ggcacgaagc gtcatcccca gaccctgcat atcatcttcg
     5221 cggccacggc ggtgcacggc attcccaaat tcaggcccga tcacacccat tccctggaca
     5281 ttgcctgggt gcctgtagcc aagctcaatg aatgtcccct atatcccgat gtgggcaagc
     5341 agttgttcgc atatttcgcc gaggggcaaa aaaggtcgct gaccttcata gaaaattgca
     5401 tggaaagggg cttctggtga gagcctttta tggtcgtctt actggtacca ccgattttca
     5461 ccttaacgtc gccaaagaaa aaggggctgc ggaaaaaccg taaccccttg attttcatgg
     5521 taggcggtac tgggattgaa ccagtgactt ctaccgtgtg aaggtagcac tctcccgctg
     5581 agttaaccgc ccatgtgacc cctctataaa ccaacggccc ggcgttttca agaaaaattt
     5641 ccggccccac gaaaagatct tccgcgcccc cggataagtc tattcccacc tgtgcggccc
     5701 cgccgtaaag tttccgcctg caccgcccac cggagcggtt gtccgcttac accgccccgg
     5761 tggagctgct tttcgcccgt gcgtcctggg gactcgatcc tcggggcata gttgaccttt
     5821 ttgtttccct cacccactcc catccccgga tggcgcctga tcgcctgatc ttgagatcgc
     5881 cttaccgtgc ccttttcttg aggtgctcca gatcggaaac gatgatcttt ccccgcttcg
     5941 tccgaacgat ccccttgtcg cggaggacct tgagctcttt gttgacgctc tcccgggaaa
     6001 cgcccaacat ctcggagagc tccttctggg tcatattgat ttgcgtttcg gaaccctgcc
     6061 gcccctcgcc ggaatcgtca cctactagat ctatgatccg ctttgccaga cgctgcgaga
     6121 gataggaaaa acatatttcg gagatgaggt catcggtctt gcgcaggcgc caggaaaggg
     6181 ccatgaggat cgcctgtatc gcatgatcgc gtttggccag gaacttcagg aaatcgccgc
     6241 gctccaggat cgccaactgt gtttcttccc cggccgacgc cgatgccgaa cggggcatgc
     6301 cgtccaacag ggccatatcg ccgaaaaatt cgccgtcctt catgacggcc acggcaatct
     6361 catcgcccag cgccgtgggc accgtga
//
LOCUS       DAOSMF010000108         6181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_13907_length_6181_cov_24.504734, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000108 DAOSMF010000000
VERSION     DAOSMF010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 6181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6181
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13907_length_6181_cov_24.504734"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1859)
                     /locus_tag="PLT63_08985"
     CDS             complement(<1..1859)
                     /locus_tag="PLT63_08985"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOD98897.1"
                     /translation="MPLVWKDLKEAGRGGLAFRMSLFILTATAVIFVAAFGYSSYYFR
                     NEILANVERNAEAVTLATVSKIEVILGGVEKVPSYLARNLQGEKPTLPVLQKQIIDLL
                     QTNPEIFGSTVAYEPYGFATETLYFAPYICRGKYGGLVPSFLGNEEYQYFYWDWYQIP
                     KELGKAVWSEPYFDEGAGNIMMSTYSVPFYTYQGGGKAFTGIVTADISLEWLMEIIEK
                     LTPHQAGYSFLISRNGVFVSHPRKDYIMQESIFSIAEAKNDPGLREIGRKMIRGGKGF
                     VTVKSHFAGQPAWMYYAPLPTLGWSIGVVFPEESLFAGINRLRAVILGIGGAGFLALL
                     IVIFVISGTITRPLRVLADKAGEIAKGNLEVEVPAVRSHDEVEALSLSFQNMQVALKE
                     YISNLAATTAAKERIESELKIARTIQMSFLPKRFPPFPEKEEFDIFAALEPAKEVGGD
                     LYDFFLVDDEHVFFSIGDVSGKGVPAALFMAVTKTLIKGIAGQSVFPSHALEQVNQEL
                     CKDNDSSMFVTVCCGILNFKTGELLFSNAGHNLPVLLSAGGEVGWLNSPEGFLLGIME
                     DASYEILRLDLNPGDTLFLYTDGVTEAMNERKEFFSDAALLKTLAACPGRDPEA"
     gene            complement(1834..>2439)
                     /locus_tag="PLT63_08990"
     CDS             complement(1834..>2439)
                     /locus_tag="PLT63_08990"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOD98898.1"
                     /translation="PADLNHKKISLWPNFQVQPQAFFRKYGLNVKTVVQGATVNLFLR
                     GGVDAASAMWYNEYHSIINAGVNEDELTAFFFDRHGLNFPEDGIYVLKETYERDREGC
                     CRFVRASRKGWEYAFAHPDEATDIVMKYIKEAHTGGNRVHQQWMLKRMKDLIQPGDAK
                     IPIGALNPGDYETVAGELRKSGLIKTIPPFGEFYAPCLERP"
     assembly_gap    2440..2539
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2540..3263)
                     /gene="hisF"
                     /locus_tag="PLT63_08995"
     CDS             complement(<2540..3263)
                     /gene="hisF"
                     /locus_tag="PLT63_08995"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOD98899.1"
                     /translation="MLSKRIIACLDVRDGKLTKGIRFQGNMDIGDPVEAARIYYEQGI
                     DEIVFYDITASSDGRNIMLEVVRRVAETIFIPFSVGGGIRSVEDMRAVILSGAEKVSV
                     NSEAVKNPDIIDEGARIFGSQCVVLGMDVKKVAVRPEIPSGYEIVIHGGRIYTGWDAL
                     QWAKEGERRGAGEICLNSIDADGTQKGYENDLTRLVSAAVGIPVIASGGAGRPEHLYA
                     VLTRGEADAALIASIVHYRTYSI"
     gene            complement(3253..3885)
                     /gene="hisH"
                     /locus_tag="PLT63_09000"
     CDS             complement(3253..3885)
                     /gene="hisH"
                     /locus_tag="PLT63_09000"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOD98900.1"
                     /translation="MIGIIDYKAGNLTSVARALQYLGESWTVTADPRELSGCDRIILP
                     GVGAAGEAMTNLRRLGMDTWLRECLAAGTPLLGICLGTQVIFSHSEENDTPCLDLVPG
                     WVRRFPDGLREGERALKIPHMGWNRVEFSRPHPVFAGLPAAEYYFVHSFYPDPADTAH
                     ILGRTEYGLRFCSALAHRNLVAVQFHPEKSGAPGLRILANFCRWGGADAQ"
     assembly_gap    3899..3998
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3999..6169
                     /locus_tag="PLT63_09005"
     CDS             <3999..6169
                     /locus_tag="PLT63_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HOD98901.1"
                     /translation="TDLQGLLEHITYFNEETGYAIIKVKVSGHKDLVACVGNLIRPTP
                     GEIVAMKGEWTNHEKYGRQFRIVTCETKTPASVYGVQKYLGSGLIKGIGPVMAKRIVG
                     KFGRKTLEIIENKTERLKEIPGIGEGRIAMIRKAWNEQKEIRTVMLFLQTHGVSVGFA
                     AKIFKQYGREAIKIVQENPYRLAADIFGIGFITADRIAEKLGLPKESLVRAEAGLLYV
                     LNQLAEQGHVYVPEPLLIQEAMNILGIEAGLIAAALENLTAARTIAVENRGDPEPRAI
                     YGLPFYLAESQTAARLKVLIAAPKQIRKIDTDKALAWVQEQLAIALAEKQIEAVRTAV
                     ENKVMIITGGPGTGKTTIINAILKIYGTVRTKILLAAPTGRAAKKMAEATGWEARTIH
                     RLLEFSPLKGGFQKHEHSPLDCDILIIDEASMIDIMLMNQLLKAVPPRATMILVGDVN
                     QLPSVGPGNVLKDIISAGVAPVVELREIFRQAKESSIIVNAHRINQGLLPEVETTPGS
                     LSDFYFIHQEEPEKVLATVLTMVKERIPKRFHLHAVNDIQVLSPMNRGVVGVTNLNAA
                     LQEALNPGEGGISRGGNTFRNRDKVMQIRNNYDKEVFNGDIGRIFHVDTEGQELKVHF
                     DDRFVTYDFSELDELVPAYAVTVHKSQGSEYPAVVIPLLTQHYMMLQRNLVYTAVTRG
                     RKLVVVIGTKKALAIAVKNNKQQQRYSLLKERLQMPAAAR"
BASE COUNT         1291 a         1830 c         1742 g         1118 t
ORIGIN      
        1 gcttcgggat ctcggcccgg acaggcggcc agggtcttca acagcgccgc gtcggaaaag
       61 aactccttgc gttcattcat ggcctccgta acgccgtcgg tatataggaa gagggtatcg
      121 ccggggttca aatcgagccg caggatttcg taggaggcgt cttccatgat gcccaggagg
      181 aaaccttcgg gtgaattgag ccaaccgacc tcgccacctg cggagagcag cacgggaaga
      241 ttgtgtccgg cattggaaaa aaggagctcc ccggttttga aattcaggat tccgcaacaa
      301 acggtgacga acatggagga gtcgttatcc ttgcagagtt cttgatttac ctgctccagg
      361 gcgtgggatg gaaagacgga ctggccggcg atgcccttga tcagggtctt ggtcaccgcc
      421 atgaacaatg ccgccgggac ccctttcccc gagacatcgc caatggaaaa gaagacatgc
      481 tcgtcatcga cgagaaagaa atcataaaga tcgccgccca cctctttcgc cggctccagg
      541 gcggcgaaga tgtcgaattc ctccttttcc ggaaagggcg ggaaacgctt ggggaggaag
      601 ctcatctgga tcgtccgggc gatcttcagc tcgctttcga tgcgctcctt ggccgcggtg
      661 gttgcggcga gattgctgat atactccttc agggccacct gcatgttctg aaaggaaagg
      721 gagagggcct ccacctcgtc gtgagaacgg accgcgggca cctccacctc cagattcccc
      781 ttggcgatct cccccgcctt gtcggcaagg acccgcaggg gcctggtgat cgtccccgaa
      841 atcacgaaga tgacgatcaa cagggcaaga aagcccgcgc cgcctatacc gagaatgacg
      901 gccctaagcc tgttgatccc cgcaaagaga ctctcctcgg ggaagaccac cccgatggac
      961 cagcccagcg tcggcagcgg ggcgtagtac atccaggccg gctggccggc gaagtggctc
     1021 ttcacggtca cgaacccctt cccaccccga atcatcttcc ggccgatttc tctcaagcct
     1081 gggtcatttt tggcctcggc gatgctgaag atactttcct gcatgatata gtccttccgc
     1141 gggtgggaaa cgaaaacccc gttgcgggag atgaggaagg aatagccggc ctgatgcggg
     1201 gtgagtttct cgatgatttc catgagccat tccagggaga tgtccgccgt cacgatcccc
     1261 gtgaaagcct tccccccccc ctggtaggtg tagaagggaa cggaataagt ggacatcatg
     1321 atgtttcccg ctccttcgtc gaagtaaggt tcgctccaga cggccttgcc cagttctttg
     1381 gggatttggt accagtccca gtagaaatac tggtattcct cgtttcctaa aaaagacggc
     1441 accaggccgc cgtatttccc ccggcagata tatggggcga aatacagggt ctccgtagcg
     1501 aagccatagg gctcataggc aacggtggaa ccgaagatct cggggttggt ctggagcaga
     1561 tcgatgatct gcttctgcag gaccggcaac gtcggcttct cgccctgaag attgcgggcc
     1621 agataggagg ggaccttctc cacgcctccc agaatcacct cgatcttgct caccgtggcc
     1681 agggtcaccg cttcggcatt cctctccaca ttggcgagga tctcattgcg aaagtaatag
     1741 gaactgtagc cgaaggcggc gacgaagatc accgccgtcg cggtgaggat gaagagggac
     1801 atccggaagg ccaggcctcc ccggccggcc tctttaaggt ctttccagac aaggggcata
     1861 gaactcaccg aaagggggaa tggtcttgat gaggccgctt ttccgcaact caccggccac
     1921 cgtctcgtaa tcaccgggat tgagggcgcc gatggggatc ttggcgtccc cgggctgaat
     1981 caggtccttc atcctcttga gcatccattg ctgatggacc cggttgccgc ccgtatgggc
     2041 ctctttgatg tatttcatca cgatgtccgt ggcctcatcc ggatgagcga aggcatactc
     2101 ccaccccttg cgggaggccc ggacaaaccg gcagcagcct tcccgatccc gctcgtaggt
     2161 ctccttgagg acataaatcc cgtcttcggg aaagttgagg ccgtgccggt caaaaaagaa
     2221 ggccgtcagc tcgtcctcgt ttacgccggc gttgatgatg gagtgatact cgttgtacca
     2281 catggcggag gcggcatcga cgccgccacg caagaataga ttgacggtcg ccccctggac
     2341 gacggtcttg acgttcaggc cgtacttccg gaaaaaggcc tggggctgaa cctggaagtt
     2401 cggccacagg ctgatctttt tgtggttcaa atccgccggn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnc gatggaatag gtccggtaat ggacgatgga ggcgatcagg
     2581 gcggcatcgg cctctccccg ggtcaggacc gcgtagagat gctccggtct ccccgccccc
     2641 ccggaggcaa ttacggggat ccccaccgcc gcggaaacca gacgggtcag gtcgttctca
     2701 taccctttct gggtgccgtc ggcatcgatg gaattcaggc agatctcccc ggcgccccgg
     2761 cgttcccctt cttttgccca ctgcagggca tcccaccctg tatagatccg gcctccgtgg
     2821 atcacgatct catatcccga ggggatctcc ggacggacgg caactttctt gacatccatc
     2881 cccaaaacga cacactgact gccgaagata cgggcgcctt catcaatgat atccgggttt
     2941 ttcaccgctt cggaattgac gctcaccttt tccgccccgg acaggatcac ggcgcgcatg
     3001 tcctccacgc tacgaatgcc tccccctacg gaaaagggga tgaagatcgt ttccgccacc
     3061 cggcggacga cctccagcat gatgttccgg ccgtccgagg atgcggtgat gtcgtagaaa
     3121 acaatctcgt cgatcccctg ctcgtaatag atccgggcgg cctccaccgg atcgccgata
     3181 tccatattgc cttggaatct gatccctttg gtcagttttc catcccgaac gtccagacag
     3241 gcgatgatcc ttttactgag catcggcgcc tccccagcgg cagaaattgg ccaggatccg
     3301 cagccccggg gcaccgcttt tctcagggtg gaactgcacc gcaaccagat tccggtgggc
     3361 aagggcggag cagaaacgaa ggccgtattc cgttcgaccc aggatgtgag ccgtatcggc
     3421 gggatcggga taaaaggaat gcacgaaata atattccgcc gccggcaagc cggcaaagac
     3481 gggatggggg cgggagaatt ccacccggtt ccagcccata tgggggatct tcaatgccct
     3541 ttccccttcc cgcaggccgt cgggaaaacg gcggacccat cccggcacca ggtccaggca
     3601 aggggtgtca ttttcctcac tatgggaaaa gatcacctgg gtgccgaggc agatccccag
     3661 caggggcgta ccggcggcaa ggcattcccg aagccaggtg tccatcccca gacggcggag
     3721 gttggtcatg gcctccccgg ccgcccccac gccgggaagg atgatccggt cacatccgga
     3781 aagctcgcgc ggatcggcgg taaccgtcca ggactcgccc aggtattgga gtgcccgggc
     3841 gacgctggtg aggttgcccg ccttatagtc aatgattccg atcacttttt acaaccttnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntc acggacctgc aggggctcct
     4021 ggaacatatc acctatttca acgaagagac cggatacgcc atcatcaaag tcaaagtctc
     4081 cgggcataag gatctggtcg cctgcgtcgg caatctcatc cggcccaccc ccggcgaaat
     4141 cgtcgccatg aagggggagt ggaccaatca cgagaaatac ggccgccagt tccggatcgt
     4201 cacctgcgag accaagaccc ccgcctcggt ttacggggtg cagaaatacc tcggctcggg
     4261 gctgatcaag ggcatcggcc ccgtcatggc caagcggatc gtcggcaaat tcggccggaa
     4321 gaccctggag atcatcgaaa acaaaacgga gcggctcaag gaaataccgg ggatcggcga
     4381 aggaaggatc gccatgatcc gcaaggcctg gaacgaacag aaggagatcc gcacggtcat
     4441 gctcttcctc cagacccacg gtgtcagcgt gggcttcgcc gccaagatct tcaagcaata
     4501 cggccgggaa gcgataaaaa tcgttcagga aaatccctat cgcctcgccg cggacatctt
     4561 cggcatcggt ttcatcaccg ccgaccggat tgcggaaaag ctgggtctgc ccaaggaatc
     4621 cctcgtccgc gccgaagcgg gccttcttta cgtcctgaat caactggccg agcaggggca
     4681 cgtttacgtc cccgagccgc tcctcatcca ggaggccatg aacatcctcg gcatcgaggc
     4741 gggcctcatc gccgcggcgc tggagaacct gacggcggca cggacgatcg ccgtggaaaa
     4801 tcggggggat ccggaaccac gggccatcta cggcctcccc ttttacctcg cggaaagcca
     4861 gaccgccgcc cgtctcaagg tcctcattgc cgcccccaag cagatccgga agatcgacac
     4921 cgacaaggcc ctggcctggg tccaggagca actggccatt gccctggcgg agaaacagat
     4981 cgaggccgtg cggacagcgg tagaaaacaa ggtcatgatc atcaccggcg gtcccggcac
     5041 gggcaagacc acgatcatca acgccatcct gaagatctac ggcaccgtcc ggacgaagat
     5101 cctcctggcc gccccgacgg gtcgggcggc gaaaaaaatg gccgaggcca cgggctggga
     5161 agccaggacg atccaccgcc tcctcgagtt cagccccctc aaggggggat tccagaagca
     5221 cgaacactcc cctctggact gcgatatcct catcatcgac gaggcatcga tgatcgacat
     5281 catgctcatg aaccagctcc tgaaggccgt tcctccgcgg gcgaccatga tcctcgtcgg
     5341 cgacgtgaac cagcttccct ccgtcggccc gggcaacgtc ctgaaggata tcatctcggc
     5401 aggggtggcg ccggtggtgg agctccggga gatcttccga caggccaagg agagctccat
     5461 tatcgtcaat gcccatcgga tcaatcaggg cctccttccg gaagtggaaa ccacgccggg
     5521 atcgttgtcg gacttctact tcatccacca ggaagagccg gagaaggtac tggccacggt
     5581 tcttaccatg gtcaaggagc ggattccgaa acggttccac ctccatgccg tcaatgacat
     5641 ccaggtgctg agtcccatga accggggggt ggtgggggta acgaacctca acgcggccct
     5701 gcaggaggcg ctgaatcccg gcgaaggggg gatatcccgg ggaggcaaca cctttcggaa
     5761 ccgcgacaag gtcatgcaga tccggaacaa ctacgataag gaggtcttca acggcgacat
     5821 cgggcggatc tttcacgtcg atacggaagg gcaggaatta aaggtccatt tcgacgaccg
     5881 tttcgtgacc tacgattttt ccgagctcga cgagctcgtc ccggcctacg ccgtgacggt
     5941 ccacaaaagc cagggctccg aatatcccgc cgtggtcatc cccttgctca cccagcacta
     6001 tatgatgctc cagcggaatc tcgtctatac cgccgttacc agagggagaa agctggtcgt
     6061 cgttatcggg acgaagaagg ccctggccat cgccgttaag aacaacaagc agcagcagcg
     6121 ctattccctc ctcaaggagc ggctgcaaat gccggcggcg gcccgttagc catcaaactt
     6181 g
//
LOCUS       DAOSMF010000109         6114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_14105_length_6114_cov_24.151675, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000109 DAOSMF010000000
VERSION     DAOSMF010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 6114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6114
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14105_length_6114_cov_24.151675"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /locus_tag="PLT63_09010"
     CDS             <1..785
                     /locus_tag="PLT63_09010"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOD98902.1"
                     /translation="VSLIDDWKGLRARAKLVIQILAVAFLIGNGIILNLFPIKVWWGY
                     GLNMILTLLWIIGITNAMNFIDGMDGLAAGLGGIISLFLAIVAFQTNQHILGWIALAM
                     LGSCLGFLPYNFRPTRPALIFLGDTGSIFLGFILASLAIVGEWADNNPIVSFSAPVLI
                     FWVLIFDMTYITVERILTGKVKNVKQWLEYVGKDHLHHRTYALLGDRRKAVLFIYLLS
                     ITLGLSAIALRYARPVDGILLVIQAFLITVVVSILDYSGRNR"
     gene            1015..1161
                     /locus_tag="PLT63_09015"
                     /pseudo
     CDS             1015..1161
                     /locus_tag="PLT63_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
     gene            complement(1223..1993)
                     /locus_tag="PLT63_09020"
     CDS             complement(1223..1993)
                     /locus_tag="PLT63_09020"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOD98903.1"
                     /translation="MYNTILLEKTSGYAVVTMNRPREMNALSREMRAELHAVLNELEN
                     DDEVKVLIITGGDYVFSAGMDIKEMAALADDEIADYFHSILKYLGKIYTYGKPVIAAV
                     GGIALGGGFNIATVCDLIIASESAIFGHPELRFGLNPLFSPLRNLVGMAKAKEISMLG
                     EPIGAREALRIGLVNKVATPERFMDEAVAMAEGLAARSGRVIAAVKKISVMAEKLDKQ
                     SSLEMEFEVMVHLFSSPERKIRMTEFMTKEFLAKMKKG"
     gene            complement(1986..2276)
                     /locus_tag="PLT63_09025"
     CDS             complement(1986..2276)
                     /locus_tag="PLT63_09025"
                     /inference="COORDINATES: protein motif:HMM:NF037081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOD98904.1"
                     /translation="MTKRELVAKVQAEFPGYRASDIAFAVYRMFDAMKEALARGERID
                     VRDFGAFLVKNRRARRARNPKTAVTLDLQERRVPFFKVGKELKERVAGRRDV"
     gene            complement(2283..3167)
                     /gene="sppA"
                     /locus_tag="PLT63_09030"
     CDS             complement(2283..3167)
                     /gene="sppA"
                     /locus_tag="PLT63_09030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HOD98905.1"
                     /translation="MRRHPILLAIIILIMMTMVFSALVFGISALTGKRKSLLPAELVA
                     AIVVEGVITDGRETIEACEEAARDDSIRAVVLRIDSPGGAVAPSQEIYEAVRELRKKK
                     KVVASIGSVGASGGYLVACGAERIVANPGSITGSISTVMHLVNAQELMHKIGVKSSVI
                     KSGKYKDIGSPAREMTPEERALLQGVIDDMNEHFLEIIVQERKMSGEKVKAIADGRIF
                     SGRQAKQVGLVDELGDFNHAVQLAGKLAGMKEKPELVYTTKKKSFWEVLFQSGLSALR
                     AEWQRQDTVPAGAYHLYE"
     gene            complement(3188..4960)
                     /locus_tag="PLT63_09035"
     CDS             complement(3188..4960)
                     /locus_tag="PLT63_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HOD98906.1"
                     /translation="MVNGSDVNETSQTQEVKSETPISKADETKAAAGREEDMGFRELY
                     EQSLQTLQLGEVVEGKVVQINPDTIMVDVGWKTEGYIPAKELRDAEGNINIAVGDSVE
                     VLVDRRDGDGNLVLSKDKAARLKIWDEVKYACENNTVMTGTIIERVKGGLSVDIGIPA
                     FLPGSQVDIRPIRDLDQFVGQQLPFNVIKYDRKRNNVVLSRRAILEKRREEEKRATLE
                     NIEEGKIVEGTIKNITDYGIFIDLGGIDGLLHITDISWGRISRPSDGFNKGQKLQVKV
                     LSFDRERERVALGLKQLTENPWETIQEKYPVNAIMPGRVVNLTDYGAFVELEPGVEGL
                     VHISEMFWTREIRHPSKVLSPGQQVQVMILEVKNEEKRISLGLKQTMSNPWETLKEKY
                     PVGTVIRGVVRNITNFGVFVGIEDGIDGLIHLSDISWTQKVKHPSDVFKKGQEIEAMV
                     LNVDVENEKFSLGVKQIEKNPWEEFTTKYGAGAVVSGKVTNITDFGIFVEIEEGIEGL
                     VHISELSQKRVKSASEIFAVGDEVTATIKSIDSKGRKIRLSIKDYETAAEGPAVHQYL
                     NNRENIGSSLGKALADARILDSDG"
     gene            complement(5035..5724)
                     /gene="cmk"
                     /locus_tag="PLT63_09040"
     CDS             complement(5035..5724)
                     /gene="cmk"
                     /locus_tag="PLT63_09040"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOD98907.1"
                     /translation="MKLGKMVIVIDGPAGSGKSTVSKLVAQKLGYHYLDTGALYRAVA
                     YMLIRAGIREDDQDNFKKILAAAEISYEKSPAGMFVRVNGEDVTAHLRNEEVSLQASK
                     ISALPAVREKLLPVQRAAGRDGGIVAEGRDMATVVFPVAEVKIFLDADENERIKRRHA
                     ELAARDGKGDYALIAEGLRRRDHQDRTRTVAPLKPHEDAIHIDTTRMSIDEVVEAVLA
                     VINRHPSREFS"
BASE COUNT         1240 a         1841 c         1526 g         1507 t
ORIGIN      
        1 tcgtcagcct gatcgacgac tggaagggat tgcgggcccg agccaagctc gtcattcaga
       61 tcctcgccgt cgcgttcctc atcggcaacg gcatcattct taatcttttc cctattaaag
      121 tttggtgggg ctacggcctc aatatgatcc tgacattatt gtggatcatc ggcatcacca
      181 acgccatgaa cttcatcgac ggcatggacg gactggccgc cggtctgggg ggcatcatct
      241 cgttgtttct ggccattgtg gcctttcaaa ccaaccagca catcctcggc tggatcgccc
      301 tggccatgct ggggagctgt ctcggatttt tgccgtacaa cttccgtccc acccgccctg
      361 ccctcatctt cctcggcgat acgggcagca tcttcctggg cttcatcctg gcgagcctgg
      421 ctattgtggg cgaatgggcg gacaacaatc ccatcgtatc cttttctgcg ccggtattga
      481 tcttctgggt cctgatcttc gatatgacct acatcaccgt ggagcggatc cttaccggca
      541 aggtgaagaa cgtgaaacag tggctggaat acgtcggcaa ggatcacctc caccaccgca
      601 cctacgcctt gctgggcgac cggagaaagg ccgtgttgtt catctacctg ctgtccatca
      661 ccctcgggct gagtgccatc gccctccgct acgcccggcc ggtggacggc attctcctag
      721 tcatccaggc ctttctcatc accgtagttg tgtccattct cgattattcg gggcgaaatc
      781 gttgaggaac cacggaatac cgcttgacaa acatattatt cgttaatagt ttaggcgaca
      841 ttttaatcca aagttgataa acaaaggagc gttgacccct tgagcaatac ctaccgctgc
      901 aagaaaaaag gaaatctaat caccgaggtc tgccacgatc agacctgcga gtggcgcctg
      961 aagaacgagg ccttcttcaa ctgcacctgg gtggcctgca acttcggtcc cttcacattg
     1021 gaagaggtgg gggaaatgat gggggtaacc agggagagga tcaggcagat cgaggccaag
     1081 gccttaaaga agctgcagca caaaaagaga cgcgatcagt tgcgggattt cgcttctccc
     1141 gacagcgagt gggatttttg acggcccggt gaattttccc gccggccggg cggcgaaaga
     1201 atcccctttt ctttgcggtc tttcaaccct ttttcatctt ggcgagaaat tctttggtca
     1261 taaactcggt catgcgtatc ttccgttccg ggctggaaaa gagatggacc atgacctcga
     1321 actccatctc cagggatgat tgtttgtcga gtttttccgc catcaccgaa atcttcttga
     1381 ccgcggcgat tactcggccg gaacgggccg ccagcccctc cgccatggcg acggcttcgt
     1441 ccataaacct ttccggagtg gccaccttat tgaccagacc gatccgcaag gcctcgcggg
     1501 ccccgatagg ctcccccagc atgcttatct ccttcgcctt ggccatgccg acgagatttc
     1561 tcaaggggct gaagagggga ttcaaaccaa accgcagctc cggatgaccg aatatggcgc
     1621 tctccgaggc aatgattaaa tcgcagacgg tggcgatatt gaagccgccc cccagggcaa
     1681 tgccccccac cgctgcgatc accggctttc cataggtgta gatcttgccc agatacttca
     1741 ggatggaatg aaagtagtcg gcaatctcgt catcggcaag ggccgccatc tccttgatat
     1801 ccatgccggc ggaaaagacg tagtcccccc ccgtgatgat cagcaccttt acctcatcat
     1861 cgttttccag ttcgttgaga accgcgtgca gttcggcccg catttcccgg gagagggcgt
     1921 tcatctcccg gggccggttc atggtgacga cggcgtaacc ggatgtcttt tccaggagga
     1981 tcgtattata catctctacg ccccgccacc cgttccttta gttccttgcc caccttgaaa
     2041 aagggcaccc ggcgctcttg aagatcgagg gtgaccgccg tctttgggtt tctcgcccgc
     2101 cgtgcccggc ggtttttcac caggaaggcg ccgaaatcac ggacatcgat tcgttcacct
     2161 cttgccaggg cctccttcat ggcgtcgaac atccgataga cggcgaaggc aatatcactg
     2221 gcacgataac cgggaaactc ggcctgcacc ttggccacca attctctttt cgtcatgatt
     2281 ttctactcgt aaaggtggta tgcccccgcc ggaacggtat cctggcgttg ccattccgcc
     2341 cgcagggccg agaggccgct ttgaaacaag acctcccaaa acgacttttt cttggttgta
     2401 tagactagtt ccggcttttc cttcattccc gccagtttgc ccgccagttg cacggcgtgg
     2461 ttgaagtccc ccagttcatc caccagtccc acctgtttgg cctggcgtcc ggaaaagatc
     2521 cggccgtcgg ctatggcctt caccttctct ccggacattt ttcgctcctg gacgatgatc
     2581 tccagaaaat gttcgttcat atcgtcgatc accccctgca ggagggccct ttcctccggt
     2641 gtcatctccc gggcggggga gccgatgtct ttgtatttgc cgcttttgat caccgatgat
     2701 ttcacgccga tcttgtgcat gagttcctgg gcatttacca ggtgcatgac cgtcgagatg
     2761 ctgccggtga tcgagccggg attggcgacg atacgttcgg cgccgcaggc gacaagataa
     2821 ccgccggagg ccccgacgga accgatggag gcaaccactt ttttcttttt tctcaattcc
     2881 cgtaccgctt cgtagatctc ctgggatggc gccacggccc ctccgggact gtcgatcctt
     2941 aggaccacgg cccggatgga atcatcccgg gcggcctctt cacaagcctc gatggtctcc
     3001 cggccgtcgg taatcactcc ttccaccacg atcgccgcca ccagttcggc tggcaacagg
     3061 gatttgcgtt tcccagtcag ggcgctgata ccgaacacga gggcggaaaa gaccatcgtc
     3121 atcatgatga ggatgatgat ggccagaagg atgggatgtc ttctcatgtt ttacggcgct
     3181 ctaccggtta cccgtccgaa tccaaaatcc gcgcatccgc cagcgccttg ccgagactgg
     3241 aaccgatgtt ttcgcggttg ttgagatact ggtgcaccgc tggcccttcg gcggccgttt
     3301 cgtagtcttt gatgctcaag cggatcttcc ggcctttgga gtcgatactc ttgatggtgg
     3361 ccgtgacctc gtcacccacg gcaaagatct ccgacgccga ttttacccgc ttttgactca
     3421 gctccgagat atgtaccagg ccttcgatgc cctcctcgat ctccacgaag atgccgaagt
     3481 cggtaatgtt ggtgaccttt ccggaaacca cggcaccagc gccgtatttg gtggtaaatt
     3541 cctcccaggg gttcttctcg atctgcttca cccccaagga aaacttttcg ttttccacat
     3601 ccacattcaa gaccatcgct tcgatctcct ggcccttctt gaagacgtca gagggatgct
     3661 tgaccttttg cgtccaggag atgtcggaca ggtgaatcag gccgtcgatg ccgtcttcta
     3721 tcccgacgaa tactccgaag ttggtgatgt ttctaaccac gcccctgatg accgttccca
     3781 ccggatattt ttccttcaag gtctcccagg ggttggacat ggtctgcttg aggcccagag
     3841 aaatacgttt ttcctcgttt ttaacttcca ggatcataac ctgaacttgc tggcccggtg
     3901 acaagacctt ggagggatgt cggatctccc gcgtccagaa catctccgag atgtgtacaa
     3961 gtccctccac accaggttcg agctccacaa aggcgccata atcggtaaga ttcaccaccc
     4021 ggcccggcat gatggcattc acggggtatt tttcctggat cgtttcccag gggttttccg
     4081 tcaactgctt gagccccagg gccacccgct cccgttcacg gtcgaaggaa aggaccttca
     4141 cctgcagctt ctgccccttg ttgaaaccgt ccgatgggcg ggaaatgcgc ccccaggaga
     4201 tgtcggtgat gtggaggagt ccgtcgatgc cgcctaaatc gataaaaatt ccgtaatcgg
     4261 tgatattttt gatcgtcccc tcgactattt tgccctcttc gatattttcc agggtcgcgc
     4321 gtttctcctc ctcacgccgc ttttccagaa tggcccttcg tgacaggacg acattgttcc
     4381 gtttccgatc gtatttgatt acattgaagg gaagttgctg accgacaaac tgatcgagat
     4441 cccggattgg ccggatatcc acctgcgagc ccggcaagaa cgccgggatg ccgatatcca
     4501 ccgacagccc tcccttgacc cgttcgatga tcgttccggt catcactgta ttattttcac
     4561 aggcatattt gacttcgtcc cagatcttga gacgcgctgc cttgtctttc gatagcacca
     4621 gattgccatc tccatcccgg cgatccacca gcacctcgac ggaatcgccg acggcgatat
     4681 tgatgttgcc ctctgcgtcc cgtaactcct tggcggggat gtagccctcg gtcttccagc
     4741 ccacatcgac catgatcgtg tccgggttta tctggacaac ctttccctcg acgacttctc
     4801 ctaactgcaa ggtttgaagg gactgttcat acaactctct gaatcccata tcttcttccc
     4861 tgcccgccgc cgcctttgtc tcatctgcct tgcttatggg cgtttcactt ttcacctctt
     4921 gagtttggga tgtttcattg acatcactac cgtttaccat taactctgta ccccctaaaa
     4981 ttttgaatat accgccgcta tgtcaaggtg cactaacaca ctgaatataa aagatcaaga
     5041 gaattcacgg gaaggatgac gattaataac ggcgagcacg gcttccacca cctcgtctat
     5101 ggacatccgg gtcgtatcga tatgtatggc gtcttcatgg ggtttgagcg gggccacggt
     5161 cctggtccgg tcctggtggt cccggcgccg caaaccttca gctatcagcg cgtagtcgcc
     5221 cttcccatca cgggcggcta attccgcgtg gcgtcttttt atccgttcgt tttcgtccgc
     5281 atcgagaaag atctttacct cggcgacggg gaagaccacc gtcgccatat cccgtccttc
     5341 ggcgacaatc ccgccatcac ggcccgccgc ccgctggacg ggaagtagtt tctcccggac
     5401 agcgggcagc gccgatatct ttgatgcctg gaggctcact tcctcgttgc gcaagtgagc
     5461 cgtcacatct tcaccgttta ccctcacgaa cattcccgcg ggtgactttt cgtaggatat
     5521 ctccgccgcc gctaaaattt ttttaaagtt atcctgatcg tcctctctaa taccggccct
     5581 gatcaacata taggccacgg cccggtagag ggcaccggta tcgagatagt ggtaacccaa
     5641 tttctgggca accagtttgc ttaccgtgct ctttcccgaa ccggccggtc cgtcgataac
     5701 gatcaccatt ttgccgagtt tcatgaatga ttcaccgcct cataagccgt tctttcccca
     5761 gcgtcggctc tcccttccgt ccgtccgcat gcggcgtcct tatatcacag gccgtttatc
     5821 atctcaatgg ctcagaaaga taaaaaaatg aacgctatct tgataatctt ccgcaaatgt
     5881 gtaaaataag aagcatgaat atccgaagac cgcccataaa catgcatatg tcccctcttc
     5941 gcgtggggag catccggtcc gcagcagcaa agtggcaacc gtttcgtagc ggcatcactt
     6001 ttctgttgac agcgatcttc gtactggcct ccgtcacggc ggcccaaggg gccttcacac
     6061 tgagcgatga aaaaaaactg ggcaaggaat tctacgacaa cctcgaaaag cacc
//
LOCUS       DAOSMF010000110         6041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_14339_length_6041_cov_21.904110, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000110 DAOSMF010000000
VERSION     DAOSMF010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 6041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6041
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14339_length_6041_cov_21.904110"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            485..1189
                     /locus_tag="PLT63_09045"
     CDS             485..1189
                     /locus_tag="PLT63_09045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HOD98908.1"
                     /translation="MKYYPVCLDIRERPCVVVGGGEVAARKVERLLECGAAVTVVAGE
                     LGDMLRELAAAGRIRHVAADYEARYLAGAFLVIGATDREEVNDCIAADCRAGGILVNV
                     VDDPRRCDFILPALLTRGSLSIAVATDGKSPALARKLRRELAWQFGPEYAAYLDLLGR
                     LRELVIAAGRSPDENREVFTALVNSPLYDHLRAGRREEARALIRDLTGLEVESIFMSG
                     PEVKLPPVPGASATME"
     gene            1282..2103
                     /gene="ccsA"
                     /locus_tag="PLT63_09050"
     CDS             1282..2103
                     /gene="ccsA"
                     /locus_tag="PLT63_09050"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HOD98909.1"
                     /translation="MDLYLLLYRLVLVAYLISTLAYMFSLLSRRVTPAKAATWIMAGA
                     CGLHALFFLGQWATRGYNPFLRLHDSLSFFALVMAGVYLAFQFRTKTRVLGVFVSPAV
                     FLLMIVSSVGIGGPLTAPETLQGGLVSLHAMLSVIGEALFALASAAGLAYLIQDGLLK
                     RRKASRLMKLFPPLRDLDRINHICLLWGFPLLTLGMVAGSIWARTVWGSHWQWDPKQI
                     WTLAAWLIYAILLHQRLAIGWKGHKSALLSVVAFVVLLAAFLVEKYFFTTIHQFY"
     gene            2128..3396
                     /gene="hemA"
                     /locus_tag="PLT63_09055"
     CDS             2128..3396
                     /gene="hemA"
                     /locus_tag="PLT63_09055"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HOD98910.1"
                     /translation="MNHKTAPVEIRERLNIACTEDFNPLGEILAIPGVEEALYLSTCN
                     RVEILVRSREGGGAEERLRELIFRHGNLSGAELARCLYVYRDETAVRHLFRVAAGLDS
                     LIMGEPQILGQVKDAYRQAVEREAAGIILNKLLHRAFRVAKRVRTETGVANHAVSVSY
                     AAVELAKKIFGTLGDKKILLVGAGEMSELAARHLLKHGGDQLFVANRTWERAVGMAAD
                     FQGTAVEFPRLGEVLREADIVITSTGAPGYILTGEMVAAALKRRKNRLLFIIDIAVPR
                     DVDPRAGDLENVYLYNMDDLQEIADENMAIRKEEAARGEEIIAAETEEFLAWFDTLAV
                     VPTIVSLREKAEAIIAGELERAEGWLRNLRPEDREEAALLAQAIVNKLLHDPMTSLKE
                     ETREGAARAYVAALRRLFRLDGAGGEKGEA"
     gene            3393..4328
                     /gene="hemC"
                     /locus_tag="PLT63_09060"
     CDS             3393..4328
                     /gene="hemC"
                     /locus_tag="PLT63_09060"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HOD98911.1"
                     /translation="MISGTLRIGTRGSALALAQANWVKERIERRHPGMGVALTVIKTK
                     GDIMQDVALVKIGGKGVFVKEIEDALLRGDIDLAVHSLKDVPAELPPGLTIAVTPERE
                     DPRDVLVSKHRVKFEAMPRRARIGTGSLRRGFQLRNIFPDVEIVPLRGNLDTRLKKVE
                     TEDLDAVVVAAAGIRRLGWVSRVTQFLPVETIIPAVGQGTLAIEVREDDGMVRKAIAF
                     LNHAPTWREAQAERSFLRRLGGGCQLPLAAYAKTEGTGIKITGLLGTIDGRVMIREEL
                     RGPADAGDALGGQLAEAILERGGRAILDMAYRDCR"
     gene            4532..>6041
                     /gene="cobA"
                     /locus_tag="PLT63_09065"
     CDS             4532..>6041
                     /gene="cobA"
                     /locus_tag="PLT63_09065"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HOD98912.1"
                     /translation="MTEAGKVYIIGAGPGDPGLITVKGAEAIGRAGVIIYDHLVSEEL
                     LGYARKDARLIYAGKKGGDHTLPQEEINRLLVAEAQQGHVVARLKGGDPFIFGRGGEE
                     AEVLAAAEIPFEVVPGVTAAVAVPAYAGIPLTHRGLTSTLAFVTGHEDPTKEKSDIDW
                     ASLAGIGTLVFFMGVKNLARITAALIAHGKDPQTPAALIRWGTTADQETVAGTLADIA
                     ALAAARGFTPPSLLVVGPVAGLRETLRWFDNRPLFGKGVVITRPEAQAESFADLLREE
                     GARPIHFPTIKIVPPPAWTELDAALDSLAGYDWIVFTSANGVRFFMARLRERGGDLRD
                     LKGIRLAVIGPATAKMLAEMGLRVDLVPAEYISEGVVQAFAGQDVRGKQILLPRAAEA
                     RDVIPAGLAQMGAQVDVITVYRTVASDRRAEELMTLIDTGKVDVITFTSPSTVHNFLA
                     IMGPEFILPAAVKIAAIGPVTAAAVRKAGLPVDIRQETYTIPGLVAGLRAFFQ"
BASE COUNT         1054 a         1953 c         2002 g         1032 t
ORIGIN      
        1 tgaggaagtt ggtcacggct tcactcaact ccgccgggag gctgatctcg atctgcaacc
       61 atcgttcttt ttgctgtgtc atgactcgcc cttcctgttc attctgtggg gaaacatatc
      121 ctttttttga ttatatttca agtaacttga aatattaccc cggccgtgat agcctgcccg
      181 gccagatgca atggagacga tctcgtaata aaccctcatg ccccgtgggg cgccacgaag
      241 catgaaaaga gcatttcatg cgaagaaaat ggcatttcat agtaagttgt tttattcctc
      301 accccttcaa gggggaggga gctaaggaaa cgagaaccat atcgccgacc ctacccttga
      361 aggagggagc tactgggagc gaaaaccata tcgcccccct atcccttgaa agggagggag
      421 atttgcttct ttttaccagg gcctcaattc agatggacac tgattatgcg ccgggggaaa
      481 gcccttgaaa tactatcccg tttgtctcga catcagggaa agaccctgcg tggtcgtcgg
      541 gggcggcgag gtggcggccc ggaaggtgga gcgcctcctg gaatgcgggg ccgccgtgac
      601 ggtcgtggcc ggggagctgg gggacatgct gcgggagctg gcggcggcgg ggcgcatccg
      661 gcatgtggcg gcggactatg aggcacggta tctggcgggg gccttcctcg tcatcggcgc
      721 cacggaccgg gaggaggtca acgactgcat cgccgccgac tgccgcgccg ggggcatcct
      781 ggtgaacgtc gtggacgacc cgcggcgctg cgatttcatc ctccccgccc tcctcacccg
      841 ggggagcctc tccatcgccg tggccacgga cggcaagagc ccggcgctgg cccggaagct
      901 gaggcgggaa ctcgcctggc aattcgggcc ggaatatgcg gcctatctcg acctgctggg
      961 ccgcctccgg gagctggtca tcgccgcggg ccggtccccg gatgaaaacc gggaggtctt
     1021 caccgctctg gtaaattcac cgctctacga ccatctccgg gcgggccggc gggaggaggc
     1081 ccgagcgctg atccgcgacc tgacgggtct ggaagtagag tcgatcttca tgtccggccc
     1141 ggaggtcaag cttccccccg tgcccggcgc gagtgcaacg atggaatgaa ttcccccttc
     1201 cgcacgcggc aggggatcac aaacgcaagc ggaatagaat gacttttacc aggtcgtcaa
     1261 atatggtatc cccataaaaa aatggactta tatcttctcc tttatcgttt ggtcctggtt
     1321 gcctatctga tcagcaccct ggcctatatg ttttccctgc tgtcccgccg ggtgacgccc
     1381 gccaaggccg ccacctggat catggccggg gcctgcggcc tccacgccct gttcttcctc
     1441 ggtcagtggg cgacccgggg ctacaacccc tttctccgcc tccacgactc cctctccttc
     1501 ttcgccctgg tcatggcggg ggtctatctg gccttccaat tccggaccaa gacccgggtc
     1561 ctgggggtct tcgtctcccc ggcggtcttc ctcctcatga tcgtctcctc cgtgggcatc
     1621 ggcggacccc tgacggcgcc ggagaccctg cagggcggcc tggtctctct ccatgccatg
     1681 ctctcggtga tcggcgaggc cctcttcgcc ctcgcctccg ccgccggcct ggcctacctg
     1741 atccaggacg gcctcctgaa gcgccgcaag gccagcaggc tgatgaagct cttcccgccg
     1801 ctccgggatc tggaccggat caaccacatc tgcctcctct gggggtttcc gctgctcacc
     1861 ctggggatgg tggcgggctc gatctgggcc cgaacggtct ggggaagcca ctggcagtgg
     1921 gaccccaagc agatctggac cctggcggcc tggctcatct acgccatcct ccttcaccag
     1981 cgcctcgcca tcggctggaa gggccacaaa tcggccctgc tctccgtagt tgccttcgtg
     2041 gtgctcctgg ccgccttcct ggtggagaag tatttcttca ccaccatcca ccaattttat
     2101 tgaaccatga atctcctcct cctcggcatg aaccataaaa cggcgcccgt ggaaatccgg
     2161 gagcgcctca atatcgcctg caccgaagac ttcaaccccc tgggggagat cctggcgatt
     2221 cccggcgtcg aggaggccct ctatctgtcc acgtgcaacc gggtggagat cctcgtccgg
     2281 agccgggagg ggggcggggc ggaggaacgg cttcgggagc tgatcttccg tcacggcaac
     2341 ctgagcgggg cggagctggc ccggtgcctc tacgtctatc gcgacgaaac cgcggtccgg
     2401 catctcttcc gggtcgccgc cggcctcgac tccctcatca tgggggaacc ccagatcctc
     2461 ggccaggtga aggacgccta ccgccaggcg gtggagcggg aggcggcggg gatcatcctg
     2521 aacaagctcc tccaccgggc tttccgggtg gccaagcgcg tccggaccga aacgggggtg
     2581 gccaatcatg ccgtttcggt gagctatgcc gccgtggagc tggcgaaaaa gatcttcggc
     2641 accctgggag acaaaaagat cctcctggta ggggcggggg agatgtcgga gctggcagcc
     2701 cggcacctcc tgaagcacgg cggggatcag ctcttcgtcg ccaaccgtac ctgggagcgg
     2761 gccgtgggga tggccgcgga cttccaggga accgccgtgg agttcccgcg gctgggggaa
     2821 gtgctgcggg aggcggacat cgtcatcacc tccacggggg cgccggggta catcctcacc
     2881 ggcgagatgg tcgccgccgc cctgaagcgg cgcaaaaacc ggctgttgtt catcatcgac
     2941 atcgccgtgc cccgggacgt ggacccccgg gcgggagacc tggaaaacgt ctatctatat
     3001 aatatggacg acctccagga aatcgccgac gagaacatgg cgatccgcaa ggaggaggcg
     3061 gcccggggag aggagatcat cgccgccgag acggaggagt tcctcgcctg gttcgacacc
     3121 ctggccgtcg tccccaccat cgtctccctc cgggaaaagg cggaggccat catcgccggg
     3181 gagctggagc gggccgaggg gtggctccgg aacctgcgcc ccgaagaccg ggaggaggcg
     3241 gcactgctcg cccaggccat cgtcaacaag ctcctccacg atcccatgac ctccctgaag
     3301 gaggaaaccc gggaaggggc ggcccgggcc tacgtcgcgg cgctgcggcg cctcttccgc
     3361 ctcgacgggg cgggcgggga gaaaggagag gcatgattag cggcaccttg agaatcggca
     3421 cgcggggcag cgccctcgcc ctggcccagg ctaactgggt gaaggaacgg atcgagcgac
     3481 ggcaccccgg catgggggtg gcgcttacgg tcatcaagac gaagggggac atcatgcagg
     3541 acgtggccct ggtcaagatc ggcggcaagg gggtcttcgt caaagagatc gaggacgccc
     3601 tcctccgggg ggacatcgat ctggcggtcc acagcctgaa ggacgtcccc gccgagctgc
     3661 cgccggggct tacgatcgcc gtcacgccgg aacgggagga cccccgggac gtcttggtct
     3721 cgaagcaccg cgtcaagttc gaggccatgc cgcggcgggc acggatcggc accggctcgc
     3781 tgcgccgggg cttccagctc cgcaatatct ttcccgacgt ggagatcgtc cccctccggg
     3841 gcaatctgga cacgcggctg aaaaaggtgg aaacggaaga tctggacgcc gtcgtggtgg
     3901 ccgccgcggg gatccggcgg ctgggctggg taagccgggt gacccagttc ctccccgtgg
     3961 agaccatcat ccccgccgta ggccagggga ccctggccat cgaggtccgg gaagacgacg
     4021 ggatggtgcg caaagccatc gcctttctca accacgcccc gacctggcgg gaggcccaag
     4081 cggaacggtc ctttctccgg cgcctcggcg ggggctgcca gctcccgctg gcggcctacg
     4141 ccaagacgga ggggacgggt atcaagatca cggggctgct ggggacgatc gacgggcggg
     4201 tcatgatccg ggaggagctc cggggaccgg cggacgccgg cgacgccctg ggggggcaac
     4261 tggcggaggc catcctcgaa cgcggcggcc gggccatcct ggacatggcc taccgggact
     4321 gccgctgaca aggccttgaa cccttaaaaa acatagctta aggaaacagc aaatgattga
     4381 aacaggcgag gtctatatta tcggcgccgg tcccggagcg gatccgggat gcaccggaat
     4441 gacggtggtg acggttattc gacttactat gaaaatgcca ttttcatgct tcatggcact
     4501 ccacggggca tgagggtctc ttggagggaa aatgactgag gcgggcaagg tttacatcat
     4561 cggcgccggc cccggcgatc cggggctgat cacggtgaag ggcgcggagg ccattggccg
     4621 ggccggggtg atcatctacg atcacctggt cagcgaggaa ctcctgggtt acgcccggaa
     4681 agacgcccgg ctgatctacg ccgggaagaa ggggggcgac cacaccctcc cccaggagga
     4741 gatcaaccgc ctcctcgtag cggaggcgca gcagggacac gtcgtggccc gcctgaaggg
     4801 gggcgacccc ttcatcttcg gccggggcgg tgaagaggcg gaggtcctgg ccgccgcgga
     4861 gatccccttc gaggtggttc ccggcgtcac ggccgccgtg gccgtccccg cctatgcggg
     4921 tattcccctc acccaccggg ggctgacctc cacgctggcc ttcgtcacgg gccacgagga
     4981 ccccaccaag gaaaaaagcg acatcgactg ggcgtccctg gcgggaatcg gcaccctcgt
     5041 ctttttcatg ggggtgaaaa acctcgcccg gatcaccgcc gccctcatcg cccacgggaa
     5101 ggatccccag accccggcgg ccctcatccg ctggggaacc acggcggacc aggagaccgt
     5161 tgcgggcacc ctggcggaca tcgccgccct ggcggcggcc cggggattca cgccgccgtc
     5221 gctcctggtc gtgggacccg tggcgggcct ccgggagacc ctgcggtggt tcgacaaccg
     5281 gcccctcttc ggaaagggcg tggtcatcac ccggccggag gctcaggcgg aatccttcgc
     5341 cgatctcctc cgggaggagg gggcccggcc cattcatttc ccgacgatca aaatcgtccc
     5401 gccgccggcg tggacggaac tggacgccgc cctggacagt ctcgccgggt atgactggat
     5461 cgtcttcacc agcgccaacg gcgtccggtt cttcatggcg cgcctccggg aacggggcgg
     5521 ggatctccgg gacctgaaag gcatccggtt ggcggtcatc ggcccggcta cggcgaagat
     5581 gctggcggaa atgggcctcc gggtggatct cgtcccggcg gaatacatct ccgagggggt
     5641 cgtgcaggcc tttgccggac aggatgtccg ggggaagcag atcctcctgc cccgggcggc
     5701 ggaggcccgg gacgtcatcc ccgcggggct tgcacagatg ggggcgcagg tggacgtcat
     5761 tacggtttac cggactgtcg cctccgaccg ccgggccgag gaactcatga cgcttatcga
     5821 taccggcaag gtcgatgtca tcacctttac cagcccctcg acggtgcaca atttcctcgc
     5881 catcatgggg ccggaattca tcctgccggc ggcggtgaag atcgccgcca tcggccccgt
     5941 caccgccgcc gccgtccgga aggcggggct gccggtggac atccggcagg agacttacac
     6001 gatccccggc ctggtcgccg gcctcagagc ttttttccag t
//
LOCUS       DAOSMF010000111         6017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_14402_length_6017_cov_25.855921, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000111 DAOSMF010000000
VERSION     DAOSMF010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 6017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6017
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14402_length_6017_cov_25.855921"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..934)
                     /locus_tag="PLT63_09070"
     CDS             complement(41..934)
                     /locus_tag="PLT63_09070"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOD98913.1"
                     /translation="MPGWVLLLLLFFLLSCPAGVNGQTIPVHHWPDRLTLCGDPVPLD
                     RRDVWEMMDQAFVVSVYSQPQTILWIKRAHRYFPYVERRLRERNLPDDLKYIAVVESA
                     LKTYATSSAQAVGPWQFINGTGKRYGLRIDRWIDERYNFERATEAALDYLTALHQEFR
                     NWPLAIAAYNCGENRMSGSLKAQGVNSFYDVDLPLETEAYIFRILAAKTILAAPAAYG
                     YAIPAAKRYPPLEYDVVDVLLSQETPVTTIASAAATNYKTIREMNPEIRQSALPPGNF
                     RICLPRGQAAGFHERLKRERS"
     gene            complement(1103..1507)
                     /locus_tag="PLT63_09075"
     CDS             complement(1103..1507)
                     /locus_tag="PLT63_09075"
                     /inference="COORDINATES: protein motif:HMM:NF023514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3568 family protein"
                     /protein_id="HOD98914.1"
                     /translation="MYKKFRRSASLLLIGMFLLSGCAAAVVGGAVVGAGTGTYMYING
                     VLKTDYNAGFDRVWSAVEKTVADMHGTDVAPAKGIGTGHIDAVINGEKVRITVTFKEK
                     ALTEVGVRVGMVGDETASRMIHTKIVANLTGR"
     gene            complement(1632..2690)
                     /gene="dnaJ"
                     /locus_tag="PLT63_09080"
     CDS             complement(1632..2690)
                     /gene="dnaJ"
                     /locus_tag="PLT63_09080"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOD98915.1"
                     /translation="MTKRCYYEILSVSRNATEEDIKKSYRRLAMQYHPDRNPGDREAE
                     ERFKEAAEAYEVLSDPQKREIYNQYGHEGLSSSGFQGFSGFEDIFSSFGDIFGDIFGF
                     SNGRGRSRTSARAGADLRYDLQISFMEAAFGLNKDIEIEKLARCHECGGSGSAPGTSP
                     EVCPRCRGRGQVTQSSGFFSISSICPNCRGQGQIITQPCRNCRGGGREKIKKTVQIKI
                     PAGVETGSRLRLRGEGEQGEYGGPNGDLYIFIHVQEHEFFQRSDDDIICRVPISFVQA
                     ALGDSIEVPTLTGTEKLKIPRGTQGGKIFRLKGKGIAHLRGFGRGDQIIETVVTIPTN
                     LNRRQEEILKEFDRISNE"
     gene            complement(2809..3690)
                     /locus_tag="PLT63_09085"
     CDS             complement(2809..3690)
                     /locus_tag="PLT63_09085"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF010568.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOD98916.1"
                     /translation="MTTPLVTSSLPGLPLAGRGKVRDIYDLDDRLLIVATDRISAFDV
                     VMPNPIPGKGRVLTLMSTFWFGTMADIIGNHVISTDPADYPEACRPYREVLAGRSMLV
                     KKARPLPVECIVRGYLSGSGWKDYRRNGTVSGIRLPAGLKESSRLPEPIFTPSTKAPE
                     GEHDLAITKEETERLIGGELTTRVIAASIAIYNRAAALAVDRGIIIADTKMEFGIYDG
                     ELIIIDELLTPDSSRFWPRDGYAEGRGQSSFDKQFLRDYLLSIPWDQRPPAPELPGDI
                     ISKTAEKYEEALKRLTS"
     gene            complement(3837..4469)
                     /locus_tag="PLT63_09090"
     CDS             complement(3837..4469)
                     /locus_tag="PLT63_09090"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOD98917.1"
                     /translation="MFLKQMQVSQMAVFAYLVGDEETGEALVIDPAADTARIATLAAD
                     NGLTIKYIVNTHGHVDHISGNRDMKESTGAQIVIHEADADMLVSTPALYLRMFGAKAS
                     PPADITVREGDTIAAGRVAFQVIATPGHSPGSMCLYGEGLVFTGDTLFVGAVGRTDLP
                     GGSWNTMARSLREKLAILPDDTKVLPGHNYGPAPTSTIGYEKRYNPYLKF"
     gene            complement(4619..5320)
                     /locus_tag="PLT63_09095"
     CDS             complement(4619..5320)
                     /locus_tag="PLT63_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOD98918.1"
                     /translation="MNDAAIMENIERIRERIARAAAGAGRRPEDVRLMAVTKTVDDER
                     IAAAIAAGVDIIGENYVQEAKRKIELMGRPCQWHLIGHLQTNKAKYAVKLFDMIHSVD
                     RPEVARELNRRAVGEGRIVPILIEVNVSGEATKRGVAREEALNLAREIAPLEHLSIRG
                     LMTMAPWFDDPEAARPYFAALRILRDRIAAADIPGVHMEELSMGMSGDFEAAVAEGAT
                     IVRIGRSLFGERPPR"
     gene            complement(5390..>6017)
                     /locus_tag="PLT63_09100"
     CDS             complement(5390..>6017)
                     /locus_tag="PLT63_09100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HOD98919.1"
                     /translation="KVASSVMGLTLSHPLILASASPRREELLRLLGLDFQVLPSRVDE
                     TFFVNERPGEHVLRLAENKAHVVALMHPQAWVLGADTIVVIDGEVLGKPADPPAAARM
                     LEKLSGRTHEVYTGFALLRLAARITVRQEVRSSVTFRNISPDEKMWYIRSPEPYDKAG
                     AYAVQGLGAFFIKEISGSYTNVMGLPLCEVVDALKTVGALSFTPPPEG"
BASE COUNT         1074 a         1832 c         1840 g         1271 t
ORIGIN      
        1 caagaaagcc gggaggcaag tgacgggaaa gtcggggcga tcaggaccgt tctctcttca
       61 gccgttcgtg aaaacccgcc gcttggcccc ggggaaggca gatgcggaag tttcccgggg
      121 gaagggcgct ttgacggatt tccgggttca tctccctgat ggtcttgtag ttggtcgcgg
      181 cggcgctggc gatggtcgtg acgggtgtct cctgggacag caaaacatct accacatcat
      241 attccagcgg tggatagcgc ttggccgccg ggatggcgta accgtaagct gccggtgccg
      301 ccagaatggt tttggcggcg aggatgcgga agatgtaggc ctccgtttcc agcgggagat
      361 caacgtcgta aaagctgttt accccctgcg ccttcaggct tccggacatg cggttctcac
      421 cgcagttgta ggcggcgatg gccaggggcc agttcctgaa ctcctgatgc aaagcggtca
      481 aataatcgag ggccgcctcc gtggcccgct cgaagttgta gcgttcgtcg atccaacgat
      541 cgatgcgcag gccgtacctc ttccccgtgc cgttgatgaa ctgccaggga cccacggcct
      601 gggcgctgga agtggcgtat gtcttcaggg cgctttccac gacggcgatg tatttgaggt
      661 catcgggcag gtttctctcc cggaggcgtc tttccacgta aggaaaatag cggtgggcac
      721 gttttatcca gaggatcgtc tggggctggc tatagaccga gacgacaaag gcctgatcca
      781 tcatttccca gacatcccgg cgatccagtg gtacggggtc gccgcagagg gtcagccggt
      841 ccggccagtg atggaccgga atggtctgcc cgttcacgcc ggcgggacag gagaggagaa
      901 aaaacaacag gagaaggagg acccatccgg gcattccggc cggctttccc ggaatggtac
      961 accgcaccct gaacttcatc tccgaccccg acgcaacaag cggcacggcc gggctcgatg
     1021 accacatctt cccgaccccc gccgcggcgc ttccctgtcg gatcttcgct tatcgatcat
     1081 ttcaatccgg gttctcgcgg cattatctgc cggtaagatt ggcgacgatt ttggtgtgaa
     1141 tcatccggga cgccgtttca tcgcccacca taccgactct gacccccacc tcggtaaggg
     1201 ctttttcctt gaaggtaacg gtgatgcgga ccttctcacc gttgatcacc gcatcgatat
     1261 ggccggtgcc gattcctttc gccggggcga cgtcggtgcc gtgcatgtcc gcgacggtct
     1321 tttccaccgc cgaccagacc cggtcgaagc cggcgttgta atccgttttg agcacaccgt
     1381 tgatatacat gtaggtgccg gtaccggcgc cgaccacggc acccccgacc acggcggcgg
     1441 cgcagccgga cagtaggaac atgccgatga gaagcagtga tgcggaacgg cgaaacttct
     1501 tgtacatttc tttttccctc ccaggcatgg atattatcgt tgccactctt ctaccctatt
     1561 aggcttgttg tgtaaaggtc attgtgacct caaagacggc ggtcgggaaa ccccgcccgg
     1621 tgcggcgatc cctactcgtt gctgatccgg tcgaattcct tcaatatttc ctcttgccgc
     1681 cggttgaggt tagtggggat ggtcacgacg gtttcgatga tctgatcgcc ccggccgaaa
     1741 ccgcggagat gggcgatgcc tttgcctttc aggcggaaga tcttgccgcc ctgggtgccc
     1801 cgagggatct tcagcttttc cgtccccgtg agcgtcggca cctcgatgct gtcccctagg
     1861 gccgcctgaa cgaaggatat gggcacccgg cagatgatgt cgtcatcgga gcgctggaag
     1921 aattcatgtt cctgcacatg gatgaagata tacagatcgc cgttggggcc cccgtattcc
     1981 ccctgttccc cttccccccg gagccggagg cgggaaccgg tctcgacccc ggcaggtatc
     2041 tttatctgca cggttttctt gatcttttcc ctaccgccgc cgcggcagtt ccggcagggc
     2101 tgggtgatga tctggccctg gccacgacag ttgggacaga tggagctgat gctgaagaat
     2161 ccgctggatt gggtcacctg gccccggccg cggcaccggg ggcatacctc cggagaagtt
     2221 ccgggggccg agccggagcc gccgcattca tggcaccggg ccagcttctc gatttcgatg
     2281 tccttgttca gaccgaaggc ggcctccatg aaggatatct gcaggtcgta cctcaggtcg
     2341 gcgccggccc gggcggaggt gcggctccgg ccccggccgt tgctgaagcc gaagatatcg
     2401 ccgaagatgt cgccgaaact gctgaagata tcctcgaatc cggagaaacc ctggaatcct
     2461 gaactgctca agccttcatg gccatactgg ttgtatatct cccgcttttg cggatcactc
     2521 aggacctcgt aagcctcggc ggcttccttg aatctttcct ccgcctcccg gtcgccggga
     2581 ttccggtccg ggtggtactg catggctaac cgccggtaac ttttcttaat atcctcttcc
     2641 gtggcgttgc ggcttacgct aaggatctcg taatagcatc ttttggtcat gggctggtct
     2701 gatccccttg ctgctcctta tggtgactca ctccgaaggc gcctgtccca ggcaaacggc
     2761 gctaatttaa taagcgccgt cggtggtgtc aaggccggcg caattatttc aggatgttaa
     2821 ccttttcagt gcctcttcat atttttcagc ggttttacta ataatatctc ctgggagctc
     2881 cggggccggc gggcgctgat cccaggggat ggacagcaga taatcccgca ggaactgttt
     2941 gtcgaagctg ctctgtcctc ttccctccgc atagccgtcc cggggccaga aacgggagga
     3001 atcgggggtg agcaattcgt cgatgatgat caattccccg tcgtatatgc cgaattccat
     3061 cttggtgtcg gcgatgatga tcccccggtc gacggccaga gcggcggccc ggttgtagat
     3121 ggcgatgctg gcggcgatga cccgggtggt caattccccg ccgatcagcc gctccgtctc
     3181 ttctttcgtg atggccaggt cgtgttcccc ttccggggcc ttcgtcgagg gggtgaagat
     3241 gggttccgga agccgggacg attctttcag tcccgccggc aggcggattc ctgatacggt
     3301 cccgttacgc cggtagtcct tccatcccga gccgctcagg tagccccgaa cgatgcattc
     3361 caccggcagg ggccgggctt tcttgaccag catgctccgc ccggccagga cctcccggta
     3421 gggacggcag gcctcgggat aatccgcggg atccgtggag attacgtggt tgccgatgat
     3481 gtccgccatg gtcccgaacc agaaggtgga cattagcgtc aaaacccgtc ctttgcccgg
     3541 gatggggttg ggcatgacga catcgaaggc ggagatcctg tccgtggcca caatgagcag
     3601 ccgatcatcc agatcgtaga tgtcccggac cttgccccgg cccgccaggg gcaaaccggg
     3661 caatgaagag gtgacaagcg gagtggtcat gatgtttccc cccaatattt gatgaacggc
     3721 atgcaactcc cgcgaaccga aggagaagct tgatgttcat gatggcgacg tttacgagtt
     3781 cactgccggt accggcggtg acgcggtttc ccccggggtg gcccaaggcc cgccggtcag
     3841 aacttcagat agggattgta ccgcttttcg tagccgatgg tcgaggtggg cgccggtccg
     3901 taattgtgtc ccggcagcac cttggtgtcg tcgggaagga tggccagttt ttcccttagg
     3961 gaacgggcca tggtgttcca cgagcccccg ggcaggtcgg tccggccgac ggcacccaca
     4021 aagagcgtgt ctccggtgaa gaccaggccc tcgccgtaga ggcacatgct gccgggcgag
     4081 tgccccggag tggcgatgac ctggaaggca acccgacccg cggcgatcgt atcgccttcc
     4141 cggacggtaa tatcggcggg cggggacgct ttggccccga acatgcgcag atacagggcg
     4201 ggcgtggaaa cgagcatgtc cgcgtccgcc tcatggatga cgatttgggc accggtgctc
     4261 tccttcatgt cccggttgcc ggatatgtgg tccacatgcc cgtgggtgtt gacgatgtat
     4321 tttatcgtca ggccgttatc cgccgccagg gtggcgatcc gggccgtatc cgcggcggga
     4381 tcgatcacca gggcctcgcc ggtttcctcg tcgcccacca gataggcgaa gacggccatc
     4441 tgactgacct gcatctgttt tagaaacatc actacctccc cgtcctgaat caggggcctc
     4501 ccctatcatg atccgggagt aaatgtcaac tcgttaagta tccgctaaca gttcagaaat
     4561 gcttgataat gaccttgcgg ggaagaaaag acacccgttt tctccttgct gcttctcctc
     4621 acctgggcgg tctctccccg aacagggagc ggccgatccg gacgatcgtg gccccctccg
     4681 cgacggccgc ctcgaaatcc cccgacatgc ccatggaaag ctcttccata tgcacgcccg
     4741 ggatgtccgc cgcggcgatc cgatcccgga ggatccgcag cgcggcgaaa tacggccgcg
     4801 ccgcttcggg gtcgtcgaac cacggggcca tggtcatcag cccccggatg gagaggtgtt
     4861 ccagcggagc gatctcccgc gccaaattca gcgcctcttc ccgggcgacg ccccgcttgg
     4921 tggcctcgcc gcttacgttg acctcgatga ggatcgggac gatccgaccc tcgccgacgg
     4981 cccgacggtt gagttcccgg gcgacctccg gacggtctac cgagtggatc atgtcgaaga
     5041 gcttcacggc gtattttgcc ttgttggtct ggagatgacc gatgagatgc cattggcagg
     5101 gacggcccat caactcgatc ttgcgttttg cctcctggac gtagttttcc ccgatgatgt
     5161 ccaccccggc ggcgatggcc gcggcaatcc gctcgtcgtc cacggtcttg gtgacggcca
     5221 tgaggcgcac gtcttcaggc cgccggcccg ctccggcggc ggcccgggcg atccgctccc
     5281 tgatccgctc gatattttcc atgatggcgg catcattcat ctttttcgtc tcattatctt
     5341 caccttccct ctggaacaac gcttgcagcg gctggggatc gccgggtttt catccctccg
     5401 ggggcggggt gaaggaaagg gcgcccacgg tcttcagggc atccaccacc tcgcagagcg
     5461 gcaagcccat gacgttcgta taggaaccac tgatttcctt gataaaaaat gccccgaggc
     5521 cctggacggc ataggccccg gccttgtcgt aaggttccgg ggagcggata taccacattt
     5581 tttcatccgg ggaaatattc cggaaggtta cggaggaccg gacctcctgc ctgacggtga
     5641 tccgagcggc aagacggagg agggcgaaac ccgtgtacac ctcgtgggtc cggccgctga
     5701 gcttctccag catccgcgcc gccgctggag gatccgccgg ttttcccagt acctcgccgt
     5761 cgatgaccac gatggtgtcc gcccccagga cccaggcctg ggggtgcatc agggccacga
     5821 cgtgggcctt gttttccgcc agcctgagga catgctcccc gggcctttcg tttacgaaga
     5881 aggtttcgtc cacccggctg gggaggacct gaaaatccag gcccagcaga cgcagcagtt
     5941 cctccctccg gggcgaggcg gaggcgagaa tcagggggtg gctgagagta agtcccatta
     6001 cactcgaggc aaccttt
//
LOCUS       DAOSMF010000112         5993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_14478_length_5993_cov_18.736612, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000112 DAOSMF010000000
VERSION     DAOSMF010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5993
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14478_length_5993_cov_18.736612"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..1310
                     /locus_tag="PLT63_09105"
     CDS             21..1310
                     /locus_tag="PLT63_09105"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HOD98920.1"
                     /translation="MREAVIVSGARTAVGEFGGSLKGVSVVEMGRLVIKEAIVRAGLR
                     PAINDFVKSARPTIFGEFDMTDIQKKNYAYDSSLIPVYFDEVIMGNVLTAGCGQNPPR
                     QAAIYAGLPEETNVITVQKVCASGMKAIALAAQAICAGDADIIVAGGMENMSNVPYAV
                     ADARWGYRMNMPFGKITDLMVHDGLWEIFNAYHMGFTAENIAAKYGITREEQDKLAFE
                     SHRRARAANASGAVADEIVPVVIPQRKGDPKVFAVDERPMDTSLEKMAKLSPVFKKDG
                     TVTAGNASGINDGATAVVVMSAEKAKELGLKPLAKIKGYASGGIDPAYMGLGPIPATR
                     KVFKKLGITMKDVGLIELNEAFAVQALGCIKELEVDLNKCNLNGSGISIGHPIGNTGA
                     RITFTLAMQMKKMGVNLGLASLCIGGGQGMSIVLEAV"
     gene            1388..2248
                     /locus_tag="PLT63_09110"
     CDS             1388..2248
                     /locus_tag="PLT63_09110"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: protein motif:HMM:NF004474.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HOD98921.1"
                     /translation="MAVKTFGVVGAGQMGNGIAQVAAYPGGLNVIMNDIADQFVDRGF
                     DTISKNLDRLVAKEKLTADQKAEILSRIKKSTNLEDMKDADFVVEAAVEREDLKLGIF
                     KKLDEVCKPDVVLATNTSSIPITRIAGATKRADKIIGMHFMNPVPVMRLVEIIRGLAT
                     SQATYDLTESLSKQFGKVPVECNDFPGFISNRILMPMINEAFCALMEGVGTPKAIDDI
                     MMLGMNHPMGPLALADLIGLDTCWAIMDVLYKGFGDSKYRPSPLLKKYVDAGWVGRKV
                     GRGVYDYTKK"
     gene            2297..3079
                     /locus_tag="PLT63_09115"
     CDS             2297..3079
                     /locus_tag="PLT63_09115"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOD98922.1"
                     /translation="MEYQNLIFAVEAGVATITFNRPKALNAMNSVTMKELYDAAMRCQ
                     TDSGIKAIILTGAGDKAFVAGADISEMQNMRPQEAMEFMELGHAALRLIETMSKPSIA
                     AINGFALGGGTEISMACDMRFASDKARFGQPEILIGLIPGWGGTQRLARLIGVGRAKE
                     IIMGGGQLDAQRAYEIGLVNRIIPAEQLMDETRKFAAKLAGLPGFALKMAKHAIVYGY
                     DLALDNANKLEVECCAQCFSTDDQKEGMKAFLEKRKPNFKGC"
     gene            3201..4349
                     /locus_tag="PLT63_09120"
     CDS             3201..4349
                     /locus_tag="PLT63_09120"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOD98923.1"
                     /translation="MNFGLTEEQQMVKDQVTKFAETQIKPIAAELDKTHRHPEEICRK
                     LGEMGIMGVAVPTEYGGAGMDTVTYVLCMIAISKACASTGVIVSVNNSLYGFPVNTYG
                     TEEQKKKFLPPVSGGKVEGCYALTEAGAGSDAGALACRAELKGNKYVVNGVKRFITNG
                     NVASYCVLAATEDPAKGYKGVLNLVVDLKNTKGFSIGKIEEKMGILASGTAELVFEDA
                     EVPVENLLGKPGQGFRQMLSGLDAGRIGIGSQACGIGKAALEDALNYAKERVQFGKPI
                     VSFQAIQFKLADMATELEAAELLLLRAAWMEDHHLDYEKESAMAKMYASDVAMKAAIE
                     GVQIFGGYGYCKEYPAERHMRDAKICQIYEGTNEIQRVVIARKLLSLR"
     gene            4436..5641
                     /locus_tag="PLT63_09125"
     CDS             4436..5641
                     /locus_tag="PLT63_09125"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOD98924.1"
                     /translation="MHPELVDTLVIPNENKIVFLIMDGLGGLPGPEGQTELETAATPN
                     LDALAKKNICGLLDPVGYGITPGSGPAHFSLFGYDPVKNNIGRGILEGAGIDFPMTER
                     DLLIRINFATVDKDGLVTDRRAGRIDNETNRRICWKLQENIRAPEAVEVIFQPVKEHR
                     AVLVIRGEGLAEEIQETDPQRLGKLPLPPLALAPVATVTERILAGVIKQAGEILADEP
                     KANMMLLRGYAKYRRYPSLYERYRLNALAIASYPMYRGIARLLGMTIAAPTETIEEEI
                     AILKQEYRNYDFFFIHVKATDSRGEDGDFDGKVKVIEQVDSLLPDIIALRPEVLVVTG
                     DHSTPAALAGHSWHPVPVILAAPTCRPDLVAYFSERDCIHGGLGRMPMMYLMGQSLAH
                     ARRLEKFGA"
BASE COUNT         1376 a         1636 c         1767 g         1214 t
ORIGIN      
        1 ttgaaaggag aagtggtagt atgagagaag ctgttattgt aagcggcgcc cggacagctg
       61 tcggcgagtt cggcgggtca ctgaagggtg tttcggttgt agaaatgggc aggctggtca
      121 tcaaggaagc aattgtcagg gcggggctcc gtccggccat taacgacttt gtcaagtcgg
      181 cccggccgac gatcttcggc gagttcgata tgacggacat tcagaagaaa aactatgcct
      241 acgacagttc cctgattccg gtgtattttg acgaggtcat catgggcaac gtcctgactg
      301 caggctgcgg ccagaatcct cctcgccagg cggcgatcta cgccggtctc cccgaggaga
      361 cgaacgtaat caccgttcag aaggtctgcg cctcggggat gaaggccatc gccctggctg
      421 cccaggccat ctgtgccggt gacgccgaca tcatcgtggc gggcggcatg gagaacatga
      481 gcaatgtccc ttacgcagtg gcggatgccc gctggggtta ccggatgaac atgcccttcg
      541 gcaagatcac cgacttgatg gtccatgacg gcctctggga gatcttcaac gcctaccaca
      601 tgggcttcac ggcggaaaac atcgccgcca agtacgggat cacccgggag gagcaggaca
      661 agctggcatt cgagagtcat cgccgggctc gggcggccaa cgccagcggc gccgtggccg
      721 atgagatcgt gcctgtcgtc attccccagc gcaagggcga tcccaaggtt ttcgccgtcg
      781 atgagcgccc tatggacacc tccctggaga agatggcgaa actgtccccg gtcttcaaga
      841 aggacggtac cgttactgcc ggcaatgcct cggggatcaa cgacggcgct accgctgtag
      901 tggtaatgag cgccgagaag gccaaggaac tgggtcttaa acccctggcc aagatcaagg
      961 gttacgcctc cggtggcatc gatccggcct acatgggcct cggtcccatc ccggctacgc
     1021 ggaaggtctt caagaaactc ggtatcacga tgaaggacgt cggcctcatc gagctgaacg
     1081 aggccttcgc ggtccaggcg ctgggctgca tcaaggaact cgaggttgat ctcaacaagt
     1141 gcaacctcaa cggcagcggg atttccatcg gacatcccat cggcaacacc ggcgccagga
     1201 tcaccttcac cctggccatg cagatgaaga agatgggcgt gaacctgggt cttgcatccc
     1261 tgtgcatcgg cggtgggcag ggaatgtcca tcgtcctcga agcggtctaa acaagcgtcg
     1321 attatgatcg gggcggccca caagggccaa cccttcatga aatcttatca tataaggagg
     1381 gttgaacatg gcagttaaga cctttggcgt cgttggcgcc ggacagatgg gaaacggcat
     1441 cgcccaggta gcggcctatc ctggcggatt gaacgtcatc atgaacgaca tcgccgatca
     1501 gttcgtggac cggggcttcg ataccatctc gaaaaacctc gaccggctgg tggccaagga
     1561 aaagctcaca gcggatcaga aggcggagat cctgagccgg atcaaaaaaa gcaccaatct
     1621 cgaagacatg aaggatgctg atttcgtggt cgaagcggcg gtggagaggg aagacctgaa
     1681 acttggcatc ttcaagaagc tcgatgaagt ttgcaagccc gacgttgtcc tggccacgaa
     1741 cacctcgtcc attcccatca cccgcatcgc tggggcgacc aaaagggcgg ataagatcat
     1801 cggcatgcac tttatgaatc ctgtcccggt gatgaggctt gtggagatca tccgcggatt
     1861 ggcgacctcg caagccacgt atgacctgac ggaaagcctg agcaaacagt tcggcaaggt
     1921 ccccgtggaa tgtaacgact tccccggctt tatttccaat cgcatcctca tgcccatgat
     1981 caacgaggcc ttctgtgccc ttatggaggg ggtgggaact cccaaggcta ttgacgacat
     2041 catgatgctc ggtatgaatc atcccatggg tcccctggcc ttggccgatc tcattggtct
     2101 ggatacctgc tgggcaatta tggatgtcct atacaagggt ttcggcgatt ccaaataccg
     2161 cccctcgccc ttgttgaaga agtatgtgga cgctggctgg gtgggtcgaa aagtcgggcg
     2221 cggcgtttac gattacacta agaagtagaa gaatcggcag cctcaatgag tgcctaatac
     2281 aaagggggta tgttttatgg aatatcagaa tcttatcttt gcagtggaag caggggtagc
     2341 gacaataacc ttcaatcgtc ccaaggccct taacgccatg aattccgtga cgatgaagga
     2401 gctctatgat gcagcgatgc gctgccaaac ggattcgggc atcaaggcga tcatccttac
     2461 gggcgccggg gacaaagcct tcgtggccgg ggcggacata tccgagatgc agaacatgcg
     2521 tccccaggag gctatggaat tcatggaact gggccacgct gccctgcgcc tcattgaaac
     2581 aatgtccaag ccgtccatcg cggcgatcaa cggtttcgcc ctgggcggcg ggacggagat
     2641 ttccatggcc tgcgatatgc gtttcgcctc cgacaaggct cgtttcggcc agccggaaat
     2701 actcattggc ctgatcccag gctggggagg aacacagagg ttggcgcgtc ttatcggcgt
     2761 gggccgggcg aaggaaatca ttatgggggg cggccagttg gatgcccagc gggcctatga
     2821 aatcggtctg gtcaaccgga tcatccccgc ggagcagctt atggacgaaa cgagaaagtt
     2881 tgccgccaag ctcgccggcc tgccgggatt cgccctgaag atggcaaaac acgccatcgt
     2941 ttatggttat gacctggccc tggacaatgc gaacaagctg gaagtggaat gttgcgccca
     3001 gtgcttcagc accgatgacc agaaggaggg gatgaaggcg ttcttggaga agcggaagcc
     3061 gaatttcaag ggttgttgac aagaaattag gcatatggag aagggtgggg cgtcaccccg
     3121 ctctctggag caggggtgac ggccgtttat atgtgtcagt ttttgacctt tcatcatttc
     3181 tatttaaggg gggtaaaagc atgaatttcg gactgactga agaacaacaa atggtaaagg
     3241 atcaggtaac caagtttgcc gaaacgcaga ttaaacccat tgctgcggaa ctggacaaaa
     3301 cccatcgcca tcccgaagaa atatgccgga agttgggtga gatgggcatc atgggcgtcg
     3361 ccgtgcccac ggaatatggc ggagcgggta tggatacggt tacctatgta ctctgtatga
     3421 tcgccatttc caaggcctgc gccagtacgg gcgtcatcgt gtcggtgaac aactccctct
     3481 atggttttcc cgtcaacacc tatgggacgg aagaacagaa aaagaagttt ttgccgcccg
     3541 tatcgggcgg caaggtcgaa ggctgctacg ccctcacgga ggcaggggcc ggctccgatg
     3601 caggcgccct ggcgtgccgg gctgaactca aaggcaacaa gtatgtcgtc aacggtgtca
     3661 agcgcttcat aaccaacggc aatgtagcca gttactgcgt cctggccgcg acggaagatc
     3721 cggcaaaagg ctacaagggc gtcctgaacc tcgttgtcga tctcaagaac accaagggct
     3781 tctccatcgg caagatcgag gagaagatgg gcatcctcgc ctcaggaacg gcggagctgg
     3841 tttttgaaga cgcggaagtg ccggtggaaa acctcctggg gaaacccggt cagggtttcc
     3901 ggcagatgct ctccggtctg gatgcgggcc gcatcggcat cggttcccaa gcctgcggca
     3961 ttggcaaggc tgctctggag gatgccctga attacgccaa ggaaagggtg cagtttggta
     4021 aacccatagt ctccttccag gcgattcagt tcaaactcgc tgatatggcc acagaattgg
     4081 aagcggcgga actgctcctc ctcagggccg catggatgga agaccatcac ctggattacg
     4141 agaaggaatc ggccatggcg aagatgtatg cctccgacgt ggccatgaag gccgccatcg
     4201 aaggtgtcca gatcttcggc ggctacggct actgcaagga atacccggcg gaacgtcata
     4261 tgcgcgacgc caagatctgc cagatctatg aaggaaccaa cgagatccag agggttgtca
     4321 tcgcccggaa gctcctcagc ctccgctaac agcgaccgta aacaataaat caggaggggc
     4381 ggatggatca tcacccgccc cctttcattt cccccttcct caaggagttt tatccatgca
     4441 tccggaattg gtcgatacgc tggtcatccc caacgaaaac aagatcgtgt ttctcattat
     4501 ggacggcctg ggcggcctcc ccggtccgga aggacaaaca gagctggaaa ccgcagcgac
     4561 gcccaatctg gatgccctgg cgaagaagaa catttgtggt cttctcgatc ccgtcggtta
     4621 cgggatcact ccgggcagcg gcccggcgca cttttccctg tttggatacg atcccgtgaa
     4681 aaacaacatc ggccggggga tccttgaagg cgccggtatc gactttccca tgactgaacg
     4741 ggacctcctg atccggatca attttgccac cgtggacaaa gacggtctgg ttacggaccg
     4801 gcgcgccggt cgcatcgaca acgaaaccaa ccgccggatc tgctggaaac tccaggaaaa
     4861 catccgggcg ccggaagccg tggaagtcat ttttcagccg gtgaaggaac accgggcggt
     4921 cctggtgatc cggggggaag gtttggccga ggagattcag gagactgatc cccaacggct
     4981 cgggaagctg ccgctgcccc ccctggccct ggcgccggtg gcgacggtta cggagcggat
     5041 cctcgctggg gtcatcaaac aggccgggga gatcctagcc gacgagccga aggccaatat
     5101 gatgctcctg cggggatatg caaaataccg ccgctatcca tccctgtatg aacgttatcg
     5161 attaaatgct ttggccatcg ccagctatcc catgtatcgg ggcatcgccc ggctcctggg
     5221 gatgaccatc gccgcaccta cggaaacgat agaagaggaa atcgccatcc tgaaacagga
     5281 ataccggaat tacgattttt tcttcattca tgtcaaagcc acagattccc ggggtgaaga
     5341 cggagatttt gacggaaagg ttaaggtcat cgaacaggtg gactccctgt tgccggatat
     5401 cattgccctg aggcctgagg tcctcgtcgt taccggcgat cactccactc cggcggccct
     5461 ggccggtcat tcctggcacc cggtgccggt catcctcgcc gcgccgacct gccgccccga
     5521 tctggttgcc tatttctccg agcgtgactg tatccacggc ggtctggggc ggatgccaat
     5581 gatgtacctc atgggccaga gcctggccca tgcccgtcgt ctggagaagt tcggcgcctg
     5641 actgggttcc gttaccggaa ctccgcctat ccggtgttgg gagtgccttg gcggagtttc
     5701 tttccccggc cttgttccgg gaaacgcttt cgccattcac ccgctgatag ttttctatta
     5761 tctcttttgt ctactgtttt ttcttgcata taactacatg tggtgttatc tatccacacc
     5821 gacaccatat atagcgtaaa attgaactaa acgcttattg ccgacgccag agaataaaat
     5881 cgaacacccg gaggaaaaga tgaacaccaa gatccgcaaa cgtgatggcc gtctggttaa
     5941 attcaaggcc gagaagatca ccaatgccat cgccaaggcc ggaatggcca cgg
//
LOCUS       DAOSMF010000113         5960 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_14588_length_5960_cov_19.604911, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000113 DAOSMF010000000
VERSION     DAOSMF010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5960
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14588_length_5960_cov_19.604911"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="PLT63_09130"
     CDS             <1..474
                     /locus_tag="PLT63_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98925.1"
                     /translation="PGAGRKKGQKDTKPRKGSRRAEAELAAQEEADKIRAMLSYGTRA
                     KAKIYQDYLQRVSQGGTLSVAEKRHMDKLGAELEAEIGEPQPTADKLDLEAYDYLRTV
                     WNDPDMDPSLRIRAAEIVFRGTDAPKGKKDEKAERAKAAGEGRFRPSAPPIRVVK"
     gene            471..2210
                     /locus_tag="PLT63_09135"
     CDS             471..2210
                     /locus_tag="PLT63_09135"
                     /inference="COORDINATES: protein motif:HMM:NF015321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HOD98926.1"
                     /translation="MTEWTTACPDWERRVMNRETLITFPPLFPQEADAALAVFKELRL
                     VDVLNKPTLGEAGRQWCFDFVASIFGAYDPGKENGGTDETAGRRLIQEFFLLIGKKNS
                     KSTSASAIMLTALIRNWRESAEFLILSPTIEIASNSFIPARDMVRADADLSDLMLVQE
                     HYRQITHRTTGAVLKVVAADNETVGGKKATGILIDELWLFGKRPNAENMLREATGGLA
                     SRPEGFIIYLSTQSDEAPAGVFKQKLDYARGVRDGKIDDPHFLPIIYEFPDAILKEKK
                     HLDPKMFYVSNPNLGASVDEQFIIREFKKAENDGHESMQGFLAKHLNVEMGLALKTQR
                     WAGADFWEAAAGDVTLDMILERSEVVVIGIDGGGLDDLLGLAVMGREAEAGTWLLWTR
                     AWCNPIALERRKSEAARYRDFEKDGDLIIVNEIGQDIKEAGDIVRRCDAAGLLDRIGV
                     DQAGIGAIVDELEAGDEKGEGAIEHDRIVGIPQGWRLNGAIKTLERKVAEKSLVHGGQ
                     ALMAWCVGNARVEPRGNAISITKQASGTGKIDPLMAAFNCCALMAMNPEARRRGSAYD
                     GLTAEQIRERMAY"
     gene            2225..2419
                     /locus_tag="PLT63_09140"
     CDS             2225..2419
                     /locus_tag="PLT63_09140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOD98927.1"
                     /translation="MKDLPDKPFLRVDEVKDLFGVSRSAVYLWIDHGKLIAGKYGGVI
                     RIKREELEKFIELGIMKPLD"
     gene            2575..3837
                     /locus_tag="PLT63_09145"
     CDS             2575..3837
                     /locus_tag="PLT63_09145"
                     /inference="COORDINATES: protein motif:HMM:TIGR01537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HOD98928.1"
                     /translation="MSDDKAWNPSLWNLYGSQSLSGENVTESTALTYSAVWNAVSLIS
                     GTIGAMPLHLMQRKGKSKNIIDNRRLYRVMHDQWNPYMTAMAGRECLMAHVLTWGNGY
                     AEIVRNGYGEVIELWPIAPDRVTPSMEGGSVVYEIRMDGKPPVTLLRERILHVPGLGY
                     DGFMGYSVIAMARKSIGLGMAMETFGSLYFGKGTHPSAVITHPTKLKDPKAFRTAVSE
                     VYAGLGNSNQLMVLEDGMKIEKVGIPPEDSQFIESRQFQIPEVARWFNLPPHKLKDLT
                     RSSFSNIESEQTSFYVDSILPWLVRLEANYNMQLLTPSDKNLSGYGRIYFKHNAEGIL
                     RGDTASRAAFYGAMLDRGVYSINEVREKEDMDPIPGGDVHLVPLNFTTLENAGKAPEP
                     KQLPAPEPPPGKGNGKDKSAELLQLPAQ"
     gene            3848..4705
                     /locus_tag="PLT63_09150"
     CDS             3848..4705
                     /locus_tag="PLT63_09150"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: protein motif:HMM:NF012783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HOD98929.1"
                     /translation="MKWYEIKNKADKAEVWIYEEIGEDFWTGGGVTAKNFQKELSAIK
                     AGQIDLHINSPGGAVFDGITIYNLIKQHPANVTTYIDGIAASIASVIALAGDKIVMAE
                     NALYMIHNPSGMVMGTAKDMRTFADTLDKVGGTMVTAYTGKTGKDADIIKALLDAETW
                     FTADEAKEMGFVDEISGEMDMAACVKFAPVMAKAGFKHIPETITGKKEPPDVKSAEKA
                     LRDVGFSHKQAKTILAEGFKGYQRDVDPPAAPRDVVPTQKPKVDKVSDLLTRAEIAAP
                     SKQTTGGKI"
     gene            4705..>5960
                     /locus_tag="PLT63_09155"
     CDS             4705..>5960
                     /locus_tag="PLT63_09155"
                     /inference="COORDINATES: protein motif:HMM:TIGR01554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HOD98930.1"
                     /translation="MKTLGQYKEDIKNLMKKAADIDAQCATENRELTEAEIALKTEIL
                     DTVDDLQKIVNVQERQARISANLEAPAAHAATVQMGAKAPEPDRKERFGSLGEQMSAV
                     VRAAVPGGQIDPRLFRAATGLNETTPSDGGFLVQTDFQSGLLQDVFQTGILPSRCRRI
                     TISGNSNGIKINGVDETSRASTRSGGILGYWKDEAAQKTASKPKFRQIELTLHKLIGL
                     CFATDELLADAVALEAFIRQAFAAEFGFLLDDAIIRGTGAGQPLGILNSGCLVTVAKE
                     GGQGADTIVWENVVKMYARLFAQSRPNAIWLINQAAEPQLMQMAMAVGTGGVPVYLPA
                     GGASQSPYATLFGRPVIACEQCSALGDVGDIIFGDFSNYILAEKGGIESAMSIHVKFD
                     YDESVFRFVLRCDGQPVRASALTPYKG"
BASE COUNT         1435 a         1516 c         1841 g         1168 t
ORIGIN      
        1 ccgggggccg gacggaaaaa ggggcagaag gatacgaagc cccggaaggg atcgcggcgg
       61 gctgaggcgg agcttgcggc gcaggaggag gcggacaaga tcagggccat gctctcctac
      121 ggcacccgcg caaaggcgaa aatctatcag gattacttgc agcgggttag tcaaggggga
      181 acattgtccg tggcggaaaa acgccacatg gacaagctgg gcgcggaatt agaggcggaa
      241 ataggggagc cacagccgac agcggacaag ttagacctgg aagcctacga ctatttgcgg
      301 acggtctgga acgatccgga catggacccg tcgttgagga tcagggcggc ggagatcgta
      361 ttcagaggga cggatgcccc gaagggcaag aaggacgaga aggcggagcg tgcgaaggct
      421 gccggtgaag ggcggttccg cccgagtgcg ccgccgatta gggtggtgaa atgacggaat
      481 ggacaaccgc ctgccccgat tgggagcgcc gggtgatgaa ccgggaaacg ctgataacct
      541 tcccgcctct gttcccacaa gaggcggacg ctgcccttgc ggtgttcaag gagctgcgcc
      601 ttgtcgatgt gctgaataag ccgacattgg gcgaggctgg gcggcaatgg tgctttgatt
      661 tcgtagcctc gatcttcggg gcctacgatc cgggaaagga gaacggcgga acggatgaga
      721 ccgcgggccg ccgcctgatt caagagtttt tcctacttat cggtaagaag aacagcaaaa
      781 gcacatctgc atccgcgatc atgttgacgg cgctcatccg caactggcgc gagtcggctg
      841 aatttctgat cctctctcct acaatcgaaa tcgcgtcgaa ctcattcatt cccgcccgcg
      901 atatggtcag agcggacgct gatctgtctg atctgatgct ggtgcaggag cactaccgtc
      961 aaatcacgca caggacaacc ggcgccgtct tgaaagttgt tgcggcggat aatgagaccg
     1021 tcggcggcaa gaaggcaacg gggatattga tcgatgaatt atggctcttt ggcaagcgcc
     1081 cgaatgcgga gaacatgctc cgggaggcaa cgggcgggct ggcatcccgt cccgagggct
     1141 ttattatcta cctgtccacg cagtcagatg aagccccggc gggcgtattc aagcaaaaac
     1201 tggactatgc ccggggcgtc cgtgacggca agatcgacga tccgcacttt ttacctataa
     1261 tttacgaatt ccccgacgca atcctaaaag agaaaaagca tctggacccg aagatgttct
     1321 atgttagcaa ccccaattta ggcgcatcag tcgatgaaca attcatcatc cgggagttca
     1381 agaaggcgga gaatgatggc catgagtcca tgcaggggtt tttggcaaag cacctcaacg
     1441 tggagatggg gcttgccttg aaaacgcagc ggtgggcagg tgccgacttc tgggaagcgg
     1501 cggcggggga tgtcacgctg gacatgatcc ttgaacgctc cgaagtggtg gtcataggga
     1561 ttgatggcgg cgggctggat gaccttttag ggcttgccgt tatggggcgc gaggcggagg
     1621 ccggaacctg gctcctttgg acgcgggcct ggtgcaaccc gattgccctc gaacggcgga
     1681 agtcagaggc ggcccgatac cgggattttg aaaaggacgg cgatctgatc attgtcaacg
     1741 agatcggaca ggacatcaaa gaggccgggg atattgtaag gcgatgcgat gcagcggggc
     1801 tgctggaccg gatcggcgtg gaccaggcgg ggattggcgc gattgtcgat gagcttgaag
     1861 cgggcgacga aaagggcgaa ggcgccattg agcatgaccg gatcgtcggc atcccgcagg
     1921 ggtggaggct aaacggcgcg atcaagacgc ttgaacgcaa ggtagcggag aagtccctgg
     1981 tccacggcgg acaggcgttg atggcgtggt gcgtcggcaa tgccagggtg gagccgcgag
     2041 ggaatgcgat cagcatcaca aagcaggcat ccggaaccgg gaagatcgac ccgttgatgg
     2101 cggctttcaa ctgctgcgct ctgatggcta tgaacccgga ggcgcggcgg cggggatcag
     2161 cttacgacgg cctaacggct gaacaaatac gtgagaggat ggcttattag ccaaaggaga
     2221 tgcaatgaag gatcttccgg acaaaccatt tttgagggtg gatgaagtaa aggacttatt
     2281 cggcgtttcg cggtcggcag tatatctctg gattgatcac ggaaaactga ttgccgggaa
     2341 atatggcggc gtgatcagga tcaagaggga agagttagag aaatttattg aattaggcat
     2401 tatgaagcct ttggactaaa aaaaccgtcc agattttcca tccgttatgt tttcttccat
     2461 ccgtcacttt cttttttacg tttccttcct tcacaatgta gccaaacttg aaaaagggcg
     2521 gctgtattgt ccatcttttc acggttcaag caattcataa tcaagaatct atccttgtca
     2581 gatgacaagg catggaatcc ctcgctttgg aatctttacg gttcgcaatc cctatccggc
     2641 gaaaacgtca ccgagtcaac ggctctgaca tatagcgccg tatggaatgc cgtttctctt
     2701 atctccggca ccatcggagc catgcctctc catctcatgc agcggaaggg taagtcaaag
     2761 aatatcatcg acaaccgccg cctgtatcgc gtcatgcatg atcaatggaa tccatatatg
     2821 accgctatgg cgggccggga atgcctcatg gcgcacgttc tgacctgggg aaacggctat
     2881 gctgaaattg tccggaacgg atacggcgaa gtcatagagc tatggccgat cgcaccggac
     2941 agggtaacgc ccagcatgga agggggaagt gttgtctatg aaatccgcat ggacggcaag
     3001 ccgcccgtaa ctctcctacg tgagcgcatc cttcatgttc ccggccttgg gtatgatggc
     3061 tttatgggat atagcgtcat tgcgatggcg cggaagtcca tcggcctggg catggcgatg
     3121 gagacgtttg gcagcctcta tttcggcaag gggactcatc cgtccgccgt catcacgcac
     3181 ccgacaaagc tcaaagaccc gaaggctttc aggacggcgg tgtcggaagt ttacgcgggt
     3241 ctggggaatt caaatcaact catggtgttg gaagatggga tgaagattga aaaagtcggc
     3301 atcccgccgg aggattccca attcatcgaa agccgtcaat ttcaaatccc ggaagtggcg
     3361 cggtggttca acctgccgcc gcataagctg aaagacctga cccgttcatc attctcgaat
     3421 atcgaatcag agcaaacgtc tttctacgtt gacagcatcc ttccatggct ggtccggctc
     3481 gaagccaatt acaacatgca actcttgacg ccctccgaca agaacctgag cggctatgga
     3541 cgcatttatt tcaagcataa cgcggaaggg atcttaagag gcgatacggc aagccgggcg
     3601 gcgttttatg gcgcgatgct ggatcggggc gtctattcga tcaatgaggt ccgcgagaaa
     3661 gaggacatgg acccgatacc gggcggcgac gttcaccttg tcccgctgaa tttcaccacg
     3721 cttgaaaatg caggcaaggc gccagagccg aagcagttac cggcaccgga accgccgccg
     3781 gggaaaggaa acggcaagga taagagcgca gagttgctac aacttcccgc tcaatagggg
     3841 gtgcaacatg aaatggtatg agatcaaaaa taaggcggac aaagccgagg tgtggattta
     3901 tgaggagatc ggggaggact tctggaccgg gggcggggtc acggcaaaga atttccagaa
     3961 agagctttcc gcaatcaagg ccgggcagat cgaccttcat atcaattcgc ctggcggggc
     4021 cgtattcgac gggatcacca tttacaacct aatcaaacag cacccggcca atgtcacgac
     4081 ttacatcgac gggatcgcgg cctccatcgc ctccgttatc gccctggccg gcgacaagat
     4141 cgtcatggct gaaaatgccc tttacatgat ccacaaccca tccggaatgg tcatgggaac
     4201 ggctaaggat atgcggactt ttgccgacac gctcgacaaa gtgggcggaa cgatggtcac
     4261 ggcctacacc ggaaagacag gaaaagacgc tgacataatc aaagcccttc tcgatgcaga
     4321 aacatggttc acggcggacg aagcaaaaga gatgggcttt gtcgatgaga tttccgggga
     4381 gatggacatg gcggcttgcg tcaagttcgc ccccgttatg gctaaggcgg gatttaagca
     4441 catcccggaa acaataaccg gcaagaagga accaccggat gtgaagtctg cggagaaggc
     4501 cctgcgtgac gtagggtttt cccacaagca ggcaaagacc attttggcgg aagggttcaa
     4561 ggggtatcag cgtgacgttg atcctcctgc ggcacctcgt gacgttgtgc cgactcaaaa
     4621 accgaaagtg gacaaggtgt ctgacttgct gaccagggcg gagatcgcag caccatcaaa
     4681 acagacaaca ggaggaaaaa tataatgaaa acattgggtc agtataaaga ggacattaaa
     4741 aacctcatga agaaagcggc ggacattgac gcacaatgcg ccacggaaaa ccgcgaactg
     4801 actgaggcgg aaatcgcgct gaaaaccgag atcctggata ccgttgacga cctgcaaaag
     4861 atcgtcaatg tccaggagcg acaggcgcga atcagcgcaa atctggaagc accggcggcc
     4921 catgccgcaa ccgttcagat gggcgcaaag gcacccgagc cggataggaa agaaaggttc
     4981 ggctctcttg gggaacagat gtcggcagtc gtccgggcgg ccgttcccgg cgggcagatc
     5041 gaccctcggc ttttccgagc ggcaacgggc ttgaacgaaa cgaccccctc ggatggtggg
     5101 ttcctcgttc agaccgattt ccagtccggg cttttgcagg atgtctttca gacggggatc
     5161 ctgccgagcc gttgccgtcg gatcactatc tccggcaatt ccaatgggat caagatcaac
     5221 ggcgtggatg aaacatcccg cgcctctacc agaagcggcg ggattctggg ctactggaaa
     5281 gatgaggccg cccagaaaac agccagcaag ccgaagttcc gtcaaatcga gcttaccctg
     5341 cacaagctga tcggcctttg tttcgcgacc gatgagctgc tggcggatgc ggtggctctg
     5401 gaggcgttca tccggcaggc gttcgcggca gagttcggtt tccttctgga tgacgcgatc
     5461 atccggggaa ccggagcggg ccagcctctg ggcatcctca acagcgggtg cctcgttacc
     5521 gttgccaagg aaggcgggca aggcgcggat acgatcgtct gggagaacgt cgtgaagatg
     5581 tatgcgcggc ttttcgcgca gtcccggccc aatgcgatct ggctgatcaa ccaggcggcg
     5641 gaaccgcagt tgatgcagat ggcgatggca gtcggcacgg gcggcgtccc ggtttacctc
     5701 cctgcgggcg gggcctcaca gtcgccgtat gcgacgctgt ttggtcgacc tgtgattgcc
     5761 tgcgagcagt gctccgctct gggcgatgtc ggcgacatca tcttcgggga tttcagcaat
     5821 tacatcctcg cggagaaggg cgggatcgag agcgccatgt cgatccatgt gaaatttgac
     5881 tatgatgagt cggtatttcg ttttgtactc cgttgtgatg gccaacctgt tcgtgcttcc
     5941 gcgcttaccc cctacaaggg
//
LOCUS       DAOSMF010000114         5921 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_14697_length_5921_cov_24.157177, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000114 DAOSMF010000000
VERSION     DAOSMF010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5921
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14697_length_5921_cov_24.157177"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            183..1181
                     /locus_tag="PLT63_09160"
     CDS             183..1181
                     /locus_tag="PLT63_09160"
                     /inference="COORDINATES: protein motif:HMM:NF016356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HOD98931.1"
                     /translation="MPLCPGKGERDVEESGTVEKIKIGISTCLLGRNVRYDGGHQWDR
                     YLTETLGRYFTYIPVCPEVEYGLPVPREAMRLVGDPAAPRLVTIRTGIDHTAGMQSWA
                     AEKLQELEQESLCGFIFKSKSPSSGMTAVKVYVPGGMPVKRGIGVFAAAFMARFPLLP
                     VEEDGRLQDPALRENFIERVFVYRRWQLLLAGRPKFRDLERFHREHKLLVMSHSVRHL
                     TILGRLVANHEQLPFDQAAAAYLQVLMEGLKLVATPKKQTNVLMHMLGYFKNVLTPED
                     KEEMLELIERYRLGRLPLIVPVTMLTHYVRKFSSPYLQEQLYLNPHPAELMLRNHS"
     gene            1259..1960
                     /locus_tag="PLT63_09165"
     CDS             1259..1960
                     /locus_tag="PLT63_09165"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HOD98932.1"
                     /translation="MPIKMRLKEEIYRNDKGHIIEKRYTMNGKPEGEYLSFFGNGQVM
                     VRLHFQGGLKNGAAESYYRDGQIREQGTFKDDHLFSAYSSYYDNGQIREQENYNAAGK
                     LHGPYRLYYRNGQVKDEEYFENGLFEGDYVSYYKNGQIREKGSFKNDKREGEYVSYYR
                     NGRVKEQARYRNGKLVGKFHVYDEKGNEIAKHLPNGQEEGEEEEHETREREQMVDDLL
                     KNLGNFNKQEKDAND"
     gene            1953..2792
                     /locus_tag="PLT63_09170"
     CDS             1953..2792
                     /locus_tag="PLT63_09170"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOD98933.1"
                     /translation="MIKTILAPTDGSEYGNTAIDYGVYIAKRLDATLTGFHVIDVRLM
                     QGPVITDISGSIGVPPYQEFYPVIEKGLEERAEVILKAFAERCAAGGVRAETRKAVGV
                     IDEAIIAEAKNHDWIVLAQRGEHFHITKGGILGSTAEAVTRHAGKPVLVTPQRYQDIE
                     SMALAYDGSPPAQNALELAVFLAEKMVWPLTLFMVTEDQGRAARWSKKAEAYLEAHQI
                     DCETVVVAGREDRAILKFIKEGAVELMVMGAYGRNRLQELLIGSTTSHVIRNSAIPVL
                     LTR"
     gene            complement(3036..>3791)
                     /gene="surE"
                     /locus_tag="PLT63_09175"
     CDS             complement(3036..>3791)
                     /gene="surE"
                     /locus_tag="PLT63_09175"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOD98934.1"
                     /translation="NDDGIYAKGLAALHHELSRDAECLVVAPEVEQSAVGHAITLFRP
                     LMVREARKNGAALGYAVAGTPADCVKLGIRALAGGPVDLVVSGINRGANVGVNVLYSG
                     TVSAATEAVILGVPSLAISLDTHKEADFGFAARFARKMAAFILGNPWPGTAVNVNIPA
                     LPEEEIKGVVVVKQGRTRLLENFERRVDPRDNIYYWLAGETQLAADESPDSDACALRR
                     GYITITPLHSDLTRYELLDDLRARVKEEKDRFF"
     assembly_gap    3792..3891
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3892..5562)
                     /gene="dnaK"
                     /locus_tag="PLT63_09180"
     CDS             complement(<3892..5562)
                     /gene="dnaK"
                     /locus_tag="PLT63_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOD98935.1"
                     /translation="MGKIIGIDLGTTNSCVAVMEGGDPVVIANQEGNRTTPSVVAFTE
                     SGERLVGQVARRQAITNSENTVYAVKRLIGRKYNVKEVQYDKGISSYRITEAANGDAQ
                     VTVRDRNYSPAEISSMVLVKMKQTAEDYLGEKITDAVITVPAYFNDSQRQATKDAGRI
                     AGLNVRRIINEPTAAALAYGLDKKKEGKIAVFDLGGGTFDISILEIGDGVFEVKSTNG
                     DTHLGGEDFDQRLIEFLATEFKKDQGIDIRSDRMALQRLKEAAEKAKIELSTAMETDI
                     NLPFITADASGPKHMNIKLTRAKLEALVEDLIAKVEAPCRTAMRDANLSPQDINEVIL
                     VGGMTRMPRVQQKVKEVFGKEPSKGVNPDEVVSVGAAIQGGVLAGDVKDVLLLDVTPL
                     SLGIETLGGVMTKLIEKNTTIPTRKSQIFSTAADNQPAVSIHVLQGERPMAADNRTLG
                     RFELVGIPPAPRGLPQIEVTFDIDANGIVHVSAKDLGTGKEQSIKITASSGLSEEEIQ
                     KLVKDAESHADEDKRKRDLVEARNHADSFVYSVEKNIKEFGDKIDAAEK"
     gene            complement(5598..>5921)
                     /locus_tag="PLT63_09185"
     CDS             complement(5598..>5921)
                     /locus_tag="PLT63_09185"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOD98936.1"
                     /translation="LLPLADSLERALQHADKSNDFESFKKGLGMLKDQLLCCLGKHGV
                     EPIDCADKQFDPNYHEALMQVTSRERDNNQIVDELEKGYLLNGRLLRPAKVTVCKRPD
                     DNGTC"
BASE COUNT         1195 a         1751 c         1664 g         1211 t
ORIGIN      
        1 ctttttacga tgccgtccca aatgggacat tttttgacct tttacgatgt cgtcacattt
       61 tgtcgttccc gcgcacgcgg gaatccggaa cctgcggaaa gagatgctga tgtctataaa
      121 ttatcattga cagcgcgggc aatcctcata taaagttcaa gcgataagcg gaggtgaaag
      181 ggttgccgct atgtccaggg aaaggggaaa gagacgtgga ggagtcggga accgtggaaa
      241 agatcaagat cggcatcagc acctgtctgc ttggccggaa tgtccgttat gacggcggcc
      301 accagtggga ccggtatctc accgaaaccc tgggacgtta ttttacctat atccccgtct
      361 gtccggaagt ggaatatggc ctgccggtcc cccgggaggc catgcgcctc gtgggcgatc
      421 cggcggcgcc ccgcttggtt accatccgca ccggcatcga tcacacggcg ggaatgcagt
      481 cctgggcggc ggaaaagctg caagaactgg agcaggagtc tctttgtggc ttcatcttca
      541 agagcaagtc ccccagttcc ggcatgaccg ccgtcaaggt ttacgttccc ggcggcatgc
      601 ctgtcaagcg cggtatcggg gtcttcgccg ccgccttcat ggcccgtttt cccctcctcc
      661 cggtggagga ggatggccgc ctccaagatc cggcgctccg ggagaatttc atcgagcggg
      721 tcttcgttta ccgccgctgg cagcttctcc tggccggccg gccgaagttc cgggatctcg
      781 agcggttcca ccgggagcac aaactgctcg tcatgtccca cagcgttagg cacctgacca
      841 tcctgggccg ccttgtcgcc aatcatgaac aactgccctt cgaccaggcg gccgccgcct
      901 atctgcaggt cctcatggaa gggttgaaac tcgtcgccac cccgaagaaa cagaccaacg
      961 tcctgatgca catgctgggc tatttcaaga acgtcctcac cccggaggac aaggaggaga
     1021 tgctggagct catcgagcgt taccgcctgg gccgccttcc cctcatcgtc cctgtcacca
     1081 tgctgaccca ttacgtccgg aaattttcct caccctacct ccaggagcaa ctctacctca
     1141 acccccatcc cgccgaactg atgctgagga accacagttg attccccggc gggtgttgaa
     1201 tcggcattga caccagcccc tgtttcccct atatggcaat taggaaagga tggcccccat
     1261 gccgatcaag atgaggctca aagaggagat ataccgcaac gacaaaggcc acatcatcga
     1321 aaaacgctat accatgaacg gcaagcccga gggggaatat ctctccttct tcggcaacgg
     1381 gcaggtcatg gtccgcctcc attttcaggg cggcctgaaa aacggcgctg cggagtctta
     1441 ctaccgggac ggccagatcc gggagcaggg aactttcaag gacgatcatc tcttcagcgc
     1501 ctattcatct tattacgaca acggccagat ccgggagcag gaaaactaca acgcggcggg
     1561 aaagctccac ggcccctacc ggctctacta ccggaacggc caggtcaagg atgaagaata
     1621 cttcgaaaac ggtctcttcg agggagacta cgtctcctat tacaagaacg ggcagatccg
     1681 ggaaaaggga agcttcaaga acgacaaacg ggaaggggaa tacgtgtctt attaccggaa
     1741 cggacgcgtc aaggagcagg cccggtaccg gaacggcaaa ttggtgggca agtttcatgt
     1801 ctacgacgaa aaggggaacg aaatcgccaa gcaccttccc aacggccagg aagagggaga
     1861 agaggaggaa cacgaaacca gggagcggga acagatggtt gacgacctgc tgaaaaatct
     1921 cggaaacttc aacaaacagg agaaggatgc caatgattaa gacgattctg gcgccgaccg
     1981 acggttccga atacggaaac accgccattg attatggcgt ttacatcgcc aaacgcctgg
     2041 atgccaccct cacgggcttt catgtcatcg atgtccggct gatgcagggg ccggtgatca
     2101 ctgatatctc cggctccatc ggggttcccc cctaccagga attctatccg gttattgaaa
     2161 agggcctgga ggaaagggcg gaggtcattc tcaaagcctt tgctgagcgt tgcgccgccg
     2221 gcggcgtccg ggcggaaacc cggaaggcgg tgggggtcat cgacgaggcg atcatcgccg
     2281 aggcgaaaaa tcacgactgg atcgtcctgg cccagcgggg agagcacttt cacatcacca
     2341 agggcggcat tctgggctcc accgccgaag cggtaacccg tcatgccggc aagccggtcc
     2401 tcgtcacccc tcagcgctac caggatatcg aaagcatggc cctggcctac gacggcagcc
     2461 ctccggccca aaacgccctg gaactggcgg tgttcctggc ggagaagatg gtctggcccc
     2521 tgacgctctt catggtcacg gaggatcagg gccgggcagc ccgctggtcc aagaaggccg
     2581 aggcctatct cgaggcacat cagatcgact gcgaaacggt ggtcgtggcg ggacgagagg
     2641 accgggcgat cctgaaattc atcaaagagg gcgccgtgga actaatggtc atgggggctt
     2701 atggccgcaa ccgtctccag gaactcctca tcggcagcac cacctcccat gtcatccgca
     2761 acagcgccat cccagtgctg ctcacccgct gacgccggca gaccaagcgg cctgcactgg
     2821 cccggaaatc catggtaaat atgttctggc cgaaacagga tgaaaacaga ggattgtttg
     2881 ttttcgtcgc ccaagtaata tcaactactt ctgcaaagaa caaatatacc aggcccagga
     2941 aacctttgac aatggcttcg ctgccgcttg tcaatttttt ccacgtaatg ccctcctctt
     3001 ggagggaagg tgctcagggt aagggatgcg gtggattaaa agaaacgatc tttttcttct
     3061 ttcacccggg cgcgtaaatc atccagaagc tcgtaacgcg tcaaatccga atggaggggg
     3121 gtgatggtga tataaccccg gcggagggcg caggcatcgg aatccggaga ttcatcggcg
     3181 gccagttgcg tctccccggc cagccagtag tagatgttgt cccgggggtc cacccggcgc
     3241 tcgaaattct ccagaagacg cgtccgcccc tgcttgacca cgaccacccc cttgatctcc
     3301 tcctcgggga gggcggggat gttgacgttc accgccgttc ccggccaggg attgcccagg
     3361 ataaacgccg ccattttccg ggcgaagcgg gcggcaaagc caaaatccgc ctctttatgg
     3421 gtatccaggg aaatcgccag ggagggaacg cctagaatga ccgcttccgt ggccgcggaa
     3481 acagtgcccg agtacaggac attgacgccc acgttggcgc cccggttgat gccggaaacg
     3541 acgaggtcca cgggaccgcc ggccagagcc cggatgccca gtttgacgca gtccgccggc
     3601 gttcccgcca cggcataacc gagcgcggcg ccgtttttcc gggcctcccg gaccatgagg
     3661 ggtcgaaaaa gggtgatggc gtggcccacg gcgctctgtt ccacctccgg tgcgaccacc
     3721 aggcattcgg cgtcccggga aagctcatga tggagcgccg ccagcccctt ggcatagatg
     3781 ccgtcgtcgt tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncttttccgc
     3901 ggcgtcgatc ttgtcgccga attccttgat gttcttttcc acgctgtaca caaaggaatc
     3961 ggcatggttc cgggcctcga cgagatcgcg cttccgcttg tcttcatcgg catgagactc
     4021 cgcatccttc acgagcttct ggatctcctc ttccgaaagg ccgctggagg cggtgatctt
     4081 gatgctctgc tccttcccgg tgcccaggtc cttcgccgaa acatggacga tgccgttggc
     4141 gtcgatgtcg aaggtaacct cgatctgggg cagaccccgg ggcgccgggg gaatcccgac
     4201 gagttcgaaa cgacccaggg tgcggttgtc cgccgccatg ggccgttcgc cctgcaggac
     4261 gtggatgctc accgccggtt gattgtccgc cgctgtggag aagatctggc tcttccgggt
     4321 gggaatggtg gtgttcttct cgatgagttt cgtcatcacc ccgcccaggg tttcgatccc
     4381 cagcgacagc ggcgtcacgt cgaggagaag gacatccttg acatccccgg ccaggacacc
     4441 accctgaatc gcggcgccga cggacacgac ctcatcggga tttactccct tgctgggctc
     4501 cttaccgaag acttccttga ccttctgctg cacccggggc atccgcgtca tcccgcccac
     4561 gaggatcacc tcgttgatgt cctgggggga aagattggcg tcccgcatcg ccgttcggca
     4621 gggggcctcc actttagcga tgagatcctc caccagcgct tccagtttcg cccgggtcag
     4681 cttgatattc atgtgcttgg gaccggaggc gtcggcggtg atgaagggga gattgatgtc
     4741 cgtctccata gccgtcgaga gctcgatctt tgccttctcc gccgcctcct tgaggcgctg
     4801 gagggccatt cgatcgcttc ggatgtcgat gccctgatcc ttcttgaatt ccgtggccaa
     4861 aaactcgatc agccgctgat cgaaatcctc tccgcccagg tgggtgtcgc cgttggtgga
     4921 cttgacctcg aataccccgt caccgatctc gaggatggag atatcgaagg ttccgccgcc
     4981 caaatcgaag acggcgattt tgccctcttt tttcttatcc agaccatagg cgagggccgc
     5041 cgccgtaggt tcgttgatga tcctccggac gttgaggccg gcgatcctcc ccgcgtcctt
     5101 ggtggcctga cgctgcgaat cgttgaaata ggccggaacg gtgatcaccg cgtcggtaat
     5161 cttttctccc aggtagtcct ccgccgtctg tttcatcttt accaggacca tggaggagat
     5221 ctccgccgga ctgtaattcc ggtcccgtac ggtcacctgg gcgtcgccgt tagccgcttc
     5281 ggtgatccgg taggagctga tgcccttgtc gtactggacc tccttgacat tatattttct
     5341 tccgatcagc ctcttgacgg catacacggt attctccgag ttggtgatgg cctgacgcct
     5401 ggctacctgt cccaccagtc tttcaccgct ttccgtaaat gcgaccaccg agggggtcgt
     5461 ccggtttcct tcctgattgg caattaccac cgggtcgccg ccttccatga ccgccacgca
     5521 ggaattggtg gttcccaagt caataccgat tatttttccc atgattatat atcctcctta
     5581 attaacccga ttcttattca acacgtcccg ttatcgtcgg gccgtttaca gacggtgacc
     5641 ttggcgggcc gcaacaatct gccgttcaac agatatcctt tttcaagttc gtccacgatc
     5701 tgattgttat cgcgttcccg gctcgtgacc tgcatgaggg cctcgtggta gttcgggtcg
     5761 aactgcttgt cggcgcagtc gatgggctcc acgccgtgct tgcctaggca gcagagaagc
     5821 tgatccttaa gcatccccaa tccctttttg aaggattcga aatcattgga tttgtcggca
     5881 tgctgcaagg cccgctccag gctgtccgcc aggggcagca g
//
LOCUS       DAOSMF010000115         5908 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_14742_length_5908_cov_23.848966, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000115 DAOSMF010000000
VERSION     DAOSMF010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5908
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14742_length_5908_cov_23.848966"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="PLT63_09190"
     CDS             <1..234
                     /locus_tag="PLT63_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOD98937.1"
                     /translation="TQRQNAEISQTRKNQVGSGDRSERIRTYNYPQGRVTDHRIGLTV
                     YSIENFLDGDIQMFIDALIAHFQAAALQGGNQG"
     gene            293..1159
                     /gene="prmC"
                     /locus_tag="PLT63_09195"
     CDS             293..1159
                     /gene="prmC"
                     /locus_tag="PLT63_09195"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOD98938.1"
                     /translation="MTVRDHLAQGTAALAASGSASPRLDAEVLLGHVLQWERSRLFLH
                     PDWELTAEEVERYRRLLARRREGEPVAYITGEKEFWSISLRVDRHVLIPRPDTEILVE
                     EVLNLLPFPGDRTPRLLEIGVGSGAVSIALAAARGDVRLIATDVSAAAVALAKGNAVR
                     AGVSGRIAFLVCSLFAGLDGLFDGIVSNPPYIAADEFADLPPEVRRYEPPGALRGGPD
                     GTAFHREIIGGAERLLRPGGFLALEIGAGQRRILEELLRRSPAYDSVSFRRDYAGCDR
                     VLRARRIQRWTR"
     gene            1147..2400
                     /gene="murA"
                     /locus_tag="PLT63_09200"
     CDS             1147..2400
                     /gene="murA"
                     /locus_tag="PLT63_09200"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOD98939.1"
                     /translation="MDKIIIQGGRRLHGEIENSGAKNAALPVITAALLTKGWNSFANI
                     PNLVDIKTIKKLLKSFGVVFAEEGSTLRVNAGDIASCLAAYDLVRTMRASVLVLGPLV
                     ARMGEARVSLPGGCAIGARPVNLHIRALQDMGAEVELQDGYVVAKARRLKGAEIYFDM
                     VTVTGTENIMMAAALADGTTILKNAAREPEVVNLAEILTGMGARIAGAGTDVIRIDGV
                     SSLHPVEGTVIADRIEAGTFMIAAGIAGGDVRINGCRPGYHGALIAKLRDAGLEIETG
                     DDFVRAIGKGRLRSVDVKTLAYPGFPTDLQAQFMSLMTVAGGLSVITETIFENRFMHV
                     SELMRLGADIVIQGSSAIVKGVPALRGAPVMATDLRASASLILAGLAAEGTTELSRVY
                     HLDRGYERIEEKFSRLGAAIERVKA"
     gene            2415..>3229
                     /gene="hisD"
                     /locus_tag="PLT63_09205"
     CDS             2415..>3229
                     /gene="hisD"
                     /locus_tag="PLT63_09205"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOD98940.1"
                     /translation="MRLIGTDEVGFAEYFRGIMNRGGGDEMLRMAVENIVREVALRGD
                     EAVFEYTLRFDGYDLNAASVQLTREEIRAAAALVAPADREVLALAAARVERFHRRQLP
                     TDWMDEEENGVRIGQRFTPLERVGIYVPGGLAAYPSTVIMAAVPARIAGVEEIVAVTP
                     LRRGSLDPLVALAMELGGVNRVFKIGGAQAVAALAYGTESVPRVDKIVGPGNAYVATA
                     KQLVFGRVDIDMIAGPSEVLVICDHTAPVPFVAADLLAQAEHDEMASAVVLTP"
     assembly_gap    3230..3329
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3331..3405
                     /locus_tag="PLT63_09210"
     tRNA            3331..3405
                     /locus_tag="PLT63_09210"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3363..3365,aa:Gly,seq:ccc)
     gene            3472..4338
                     /locus_tag="PLT63_09215"
     CDS             3472..4338
                     /locus_tag="PLT63_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98941.1"
                     /translation="MGDGGGNRRRVEEIVAAVTLCMALLFICTPLARDHLQRARAQEC
                     LAQRYEIERAEAAYRLEKRAASKNFAALAEAKLIAREPACPAGGVYVWLRRQPRPLLG
                     CSIHDAPAPVRDDAPPLYAADFDDLSGLTILSGGWHTVNGVLASRPGGDNRLKFGETS
                     WKDYEIRVNALLLEGDGYSIYYRADGRPDISGYCFQYHPGRGNKFLVRRVSGGVDQHP
                     LQFADMPAGFAIYNQGHEISIAVRGDHHTIKIDDRTVLDFNDAAFASGAGGFQTRGRS
                     EANFDALSVRQR"
     gene            4348..4884
                     /locus_tag="PLT63_09220"
     CDS             4348..4884
                     /locus_tag="PLT63_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98942.1"
                     /translation="MDDERREFLCRGCGKPILGLQGEQLEKEGHYYCPRCLIALAESY
                     IEEPPPEKSWWRLLRETRAWRLSMYLVTAVSLGAVVYQSPRFLGSWRELPPIRAGGIY
                     ATGAKTDKCIHNLWRAVQERQQGKMSSEAPLVCPASGRTYVVVPGADPEIHCPDPGRH
                     GFRDIVVSPGRPVPELKR"
     gene            complement(4906..>5908)
                     /locus_tag="PLT63_09225"
     CDS             complement(4906..>5908)
                     /locus_tag="PLT63_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98943.1"
                     /translation="TSTDLKIAGNYREYAKAAYNAEAGVEFFIDYLRTDANAAVKTVT
                     YPASNGGTVTVDITPPPGFSFSSSVDIICMDSAQRLYKFEMTGTAESNASKTIEVFFK
                     QAGILPLSVDGAVTTFGAGTTVNLKSGGGSGYNIDGNNYPLPANPNCTGNACRTASSP
                     TGAMPGLYAPAVAPTVTGNLSFLAGNPPLRVGGGIQTEQEWTDFVNQVLANPNNYQTT
                     GTGTRAQPGITVVGSGSSLTVSTNGSGLVIVQDGGSFILTGTKLFEGVVVLMGNNSTI
                     SLGGASILYGTLVTIGHTNRTLDFSGTDFRFSRQAISNIGNLDNLQRLRRVSWRNVS"
BASE COUNT         1024 a         1711 c         1952 g         1121 t
ORIGIN      
        1 acccagcgcc agaacgcgga gatctcccag acgcggaaga atcaggtggg cagcggggac
       61 cgcagcgagc ggatccggac ctacaattat ccccagggac gggtgacgga tcaccgcatc
      121 ggcctcaccg tttacagtat cgagaatttt ctggacggcg acatccagat gtttatcgat
      181 gccctgatcg cccatttcca ggcggccgcc ctgcagggag gaaatcaagg ttgatgatga
      241 tggccccagg acggcatgcc ggcctccgaa gatggtgacc ggccccgcaa ccatgacagt
      301 ccgggatcac ctggcccagg ggaccgccgc cctggccgcg tctgggagcg ctagtccccg
      361 cctggacgcc gaggtcctgc tggggcatgt cctgcaatgg gagcggtccc ggctttttct
      421 ccatcccgac tgggaactga cggcagagga agtggagcga taccgccgat tgctggcccg
      481 gcgccgggag ggcgagccgg tggcctacat caccggggaa aaggagttct ggtcgatctc
      541 cctccgtgtc gatcgtcatg tccttatccc ccgtccggat acggagatcc tggtggagga
      601 agttctgaac ctcctgccgt tccccggcga ccggacgccc cggctcctgg agatcggcgt
      661 cggcagtggc gccgtcagca tcgccctggc ggcggcccgg ggagatgtcc gactgattgc
      721 cacggacgtc tccgccgccg cagtggccct ggccaagggc aacgccgtcc gggcgggcgt
      781 gtcgggaaga atcgccttcc tcgtctgctc cctttttgcc ggtctggacg gacttttcga
      841 cggcatcgtc tccaatcccc cctacatcgc cgccgacgaa tttgccgacc tgccgccgga
      901 ggtgcggcgc tacgaacccc caggcgccct tcggggcggg ccggacggaa ccgccttcca
      961 tcgagaaata atcggcggcg ccgaacgtct gctccgcccc ggcggttttc tggcgctgga
     1021 gataggggcg ggccagcgac gtattcttga ggaactattg cggaggtcgc cggcttatga
     1081 tagtgtttct ttccgccggg actatgccgg ctgcgaccgg gtgctccggg caaggagaat
     1141 tcagcgatgg acaagataat cattcagggc ggccggcggc tgcacggaga gatagagaac
     1201 agcggcgcca agaacgccgc gttgcccgta atcacggcgg cgctcctgac gaagggctgg
     1261 aactcctttg ccaacatccc caacctcgtc gatatcaaga ccataaagaa actcctgaaa
     1321 agtttcggag tcgtttttgc cgaagaagga tcaaccctcc gggtcaatgc cggagacatc
     1381 gcctcgtgcc tggcggccta tgatctggtc cggacgatgc gggcttcggt actggtcctg
     1441 ggaccactgg tggcgagaat gggggaggcg cgggtctccc tgcccggcgg ctgtgctatc
     1501 ggcgcccggc cggtcaatct ccatatccgg gccctccagg acatgggagc ggaggtggag
     1561 ctgcaagacg gctatgttgt cgccaaggcc cggcggctga agggggcgga aatatacttc
     1621 gacatggtta ccgttacggg gacggagaat attatgatgg cggccgccct cgcggatgga
     1681 acgaccattt tgaaaaacgc cgcccgggaa ccggaggtgg tgaatctggc ggaaatcctc
     1741 accggcatgg gggccaggat tgccggagcc ggtacggatg tcattcgcat cgacggcgtt
     1801 tcctccctgc acccggtgga gggaacggtc atcgccgacc ggatcgaggc ggggaccttc
     1861 atgattgccg ccgggatcgc cggcggggat gtccggatca acggctgccg tccggggtac
     1921 cacggtgccc tcatcgccaa gctccgggat gcgggcctgg agatcgagac gggagacgat
     1981 ttcgtccgcg ccatcggtaa gggccgcctg cggagcgtgg acgtcaagac cctggcctat
     2041 ccgggttttc ccacggatct ccaggcccaa ttcatgtccc tcatgaccgt ggccggcggt
     2101 ctcagcgtca ttacggagac gatcttcgag aaccgtttta tgcacgtcag tgaattgatg
     2161 cgtctggggg cggatattgt catccagggc agcagcgcca tcgtcaaggg ggtgccggcc
     2221 ctgcggggtg ccccggtcat ggccacggac ctccgggcct cggcctcgct gatcctggcc
     2281 ggcctggcgg cggaaggaac aacggaattg tcgcgggttt atcatctcga tcgcggttac
     2341 gagcgcatcg aagaaaaatt ctcccggctc ggggcagcca tcgagcgggt caaggcgtaa
     2401 cagagattgt tcccatgaga ctgatcggta cggacgaagt gggatttgcg gagtatttcc
     2461 ggggcatcat gaaccgcggc ggcggagacg agatgctgcg gatggcggtg gaaaacatcg
     2521 tccgggaggt ggcgcttcgg ggggatgaag cggtttttga atatactttg agattcgacg
     2581 gttatgatct gaatgcagca tcggtacagt tgacccggga ggaaataagg gctgcggccg
     2641 ccctggtggc cccggcggac cgggaggtat tggcgctggc cgcggcccgg gtggagaggt
     2701 ttcaccgccg gcagttgccg acggactgga tggacgaaga ggaaaacggg gtgaggatcg
     2761 gtcagcggtt cacccccctg gaacgggtgg gtatttacgt ccccggcggc ctggccgctt
     2821 acccgtccac cgtgattatg gccgccgttc cggcccggat cgccggggtg gaggagatcg
     2881 ttgcggtgac gcccctccgg cgggggtccc tcgaccccct ggtggccctg gccatggaat
     2941 tgggcggcgt gaaccgggtc ttcaagatcg ggggcgccca ggccgtggcc gccctggcct
     3001 acggaaccga gtcggtgccc cgggtcgaca agattgttgg gccgggaaac gcctatgtgg
     3061 cgacggccaa acaactggtc ttcggacggg tggacatcga tatgatcgcc ggtcccagcg
     3121 aggttctcgt catctgcgac catacggcgc cggttccctt tgtcgccgcc gacctccttg
     3181 cccaggcgga gcatgacgaa atggccagcg cggtggtgtt gacccccgan nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng gcgggcgtaa ttcagcggta gaatgtcagc
     3361 ttcccaagct gaaagtcgag ggttcgattc ccttcgcccg ctccattaga aacgtcaagg
     3421 gggcacggat atggatccgg ccccttttta tttgtccggg ggaggcggag aatgggtgat
     3481 gggggcggaa accgccgtag ggtcgaggag atagttgcgg ccgtcacgct ctgtatggcc
     3541 ctgctgttta tctgcacccc cctggccagg gatcatctcc aacgagcgcg ggcccaggag
     3601 tgcctggccc agcgttatga gattgagcgg gcggaggcgg cctaccggct ggaaaagcgg
     3661 gccgcgagca agaacttcgc cgctttggcc gaggcgaagc ttattgcccg ggaaccggca
     3721 tgtcccgcgg gaggcgttta cgtctggctg cggcggcaac cccggccgct gctcggctgt
     3781 tccatccacg atgcccccgc cccggttcgg gacgacgccc caccgcttta tgccgctgat
     3841 ttcgatgacc tgagcggcct tacgatcctc tccggtggat ggcatacggt aaacggcgtc
     3901 ctggcctccc gccctggtgg ggataatcgc ttaaaattcg gtgaaacttc ctggaaggac
     3961 tacgaaattc gggtgaatgc cctcctccta gagggtgacg gctacagcat ctattaccgt
     4021 gccgacggtc gcccggacat ttcgggttat tgttttcagt accatcccgg caggggcaat
     4081 aaattcctcg tccgcagggt ttccggcggt gtcgaccagc atcccctcca attcgccgac
     4141 atgccggcgg gatttgccat atataatcag ggccatgaaa tcagtatcgc cgtccggggt
     4201 gaccatcata ccatcaagat cgacgaccgg accgttctcg atttcaacga tgccgccttt
     4261 gcttccggcg ccggcggttt tcagacccgg gggaggtccg aggctaattt cgacgcgctg
     4321 agcgtacggc agcggtgaga gatgacgatg gacgatgaac ggcgggaatt tttgtgccgg
     4381 ggttgcggca agcccatttt gggccttcaa ggcgagcaac ttgagaagga ggggcattac
     4441 tactgtccgc gctgtcttat tgccctggcg gagagttata tcgaggaacc gcccccggag
     4501 aaatcgtggt ggcggctgct gcgggagacc agggcctgga ggctctccat gtacctggtg
     4561 acggcggttt ccctgggtgc cgtcgtctat cagtcccccc gcttcctggg gtcgtggcgg
     4621 gaactgccgc ccattcgcgc cggggggatc tatgccaccg gcgccaaaac cgataaatgt
     4681 atccataatc tctggcgggc cgtccaggag cggcagcaag ggaagatgtc ctccgaggcg
     4741 cccctggtct gtcccgccag cggcaggacc tatgtagtcg ttcccggagc ggatccggag
     4801 attcactgtc ccgatccggg gcgtcacggt tttcgggata tcgtggtttc gcccggccgg
     4861 cccgttccgg agttgaaaag gtaggcgcga tcctatcatc ggacctcagg agacattccg
     4921 ccaggaaacg cgccggagcc gctgcaagtt gtcgagattg ccgatattgc ttatggcctg
     4981 acggctgaat ctaaagtccg tgccgctgaa atcgagggtc cggttcgtat ggccgatcgt
     5041 cacgagggtc ccgtagagga tcgaggcgcc gcccaggctg atggtgctgt tgttgcccat
     5101 caggacgacc accccttcga aaagcttggt gccggtgagg atgaaactgc cgccgtcctg
     5161 gactatcacc aagccggagc cgttggtact tacggtaagg ctcgaaccgc ttcccaccac
     5221 ggtgatgccg ggctgggccc gggtgcccgt gccggtggtt tgatagttgt tggggttggc
     5281 caggacctga ttcacgaagt ccgtccattc ctgttccgtc tggatgccgc cgccgacccg
     5341 caggggcggg ttcccggcga ggaaggagag attgcccgtc acggtcggcg cgaccgctgg
     5401 cgcgtagagg ccgggcatcg ccccggtcgg ggaggacgca gtccggcagg cattgccggt
     5461 gcagttggga ttggcgggga gggggtaatt gttgccgtcg atattatagc cgctgccgcc
     5521 tccgctctta agattgaccg ttgttccggc accgaatgtg gttacggcgc cgtccacgct
     5581 gaggggcagg atccccgcct gcttgaaaaa gacctcgatg gtcttggagg cattgctctc
     5641 tgccgtaccc gtcatttcga acttatacag ccgctgggcg ctgtccatgc agatgatgtc
     5701 cacggaggaa ctgaaggaaa accctggcgg cggggttatg tccacggtga ccgtcccgcc
     5761 gttgctggcg ggataggtga cggtcttgac ggcggcattg gcgtcggtgc gcagataatc
     5821 tatgaagaac tccactcctg cttcggcatt gtaggccgct ttggcatact cccggtaatt
     5881 gccggcgatc ttgagatccg tggaggtc
//
LOCUS       DAOSMF010000116         5800 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_15102_length_5800_cov_27.923934, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000116 DAOSMF010000000
VERSION     DAOSMF010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5800
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15102_length_5800_cov_27.923934"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..1074
                     /locus_tag="PLT63_09230"
     CDS             151..1074
                     /locus_tag="PLT63_09230"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HOD98944.1"
                     /translation="MITIRRLEDISAELKGSFVTIGNFDGVHRGHQYLFQNLVRQARN
                     AGAGAVVITFDPHPKMVLHPERRPFYLITTLEEKIKLIATQGVDGLLVIPFSPEFSRT
                     TAQEFVCDLLWDKLRIRKILIGHDYTFGRGKEGNEAFLVARGEHLGFAVEVMNAFTSN
                     GDIISSTLVRNAILAGDVKKAAACLGRPYNLVGVVVAGHRRGAGLGFPTANIQPEKVL
                     LPARGVYAALTKLAGRTYRSVLNIGLNPTFADEQLSIEVHLLDFNEDIYGRTLEVLFW
                     EQLREEVKFNGPAALIAQIERDVARARELLP"
     gene            1071..1787
                     /gene="nfi"
                     /locus_tag="PLT63_09235"
     CDS             1071..1787
                     /gene="nfi"
                     /locus_tag="PLT63_09235"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: protein motif:HMM:NF008629.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HOD98945.1"
                     /translation="MKIRPLHSWDIPRGNAVALQNNLRTQLVLADPDPAEDRITCIAG
                     ADISYARHSDLFYAVVVLLSYPGLAVREEARAIGRVSFPYVPGLLSFREGPILLQAME
                     KLSVVPDVVMFDGHGIAHPRGFGLASHLGLWLDLPSIGCAKSRLVGDYREPGPDAGEE
                     EPLVFKGKIIGMVLRTKRRVRPVFISPGHRISLARARDVARWCCCGYRIPEPVRQAHL
                     AVNRLRLAGGIANGELRRCR"
     gene            complement(1759..4707)
                     /locus_tag="PLT63_09240"
     CDS             complement(1759..4707)
                     /locus_tag="PLT63_09240"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal domain-containing protein"
                     /protein_id="HOD98946.1"
                     /translation="MFPVTKKKTVLIIGMLLAFAVAAAAVGLHVFRQLQNLEAYRQTI
                     EAYARRALGRDLQYESARLSFRFGPALTFTGVKIAEKSGPEPFASAAEIAVRIAVLPY
                     ILEKRLVVREIRLEEPRMRIVRDREGRWNIADLFTATEAPEVDVGGITAAGGEITLVD
                     TTIKEGVLVIKAIDFHLERWERGATAAFSLAGTLAGKSGDGRLSLAGKVRLAKKTEDI
                     LDSSLIRTKLRTEQLDLAGPWPWYRRWTPFQKLGGLLTAELVLNGPGHDFTASGSLEI
                     TDPELAYPPVFPETIKAARLRLTCDVHRTPGAVVMSRAEAALDGLKVQGKMTLKDLLS
                     PDPFVEATTSVADFDLERHGNLIPYGIIPASTGNFIRKYVRAGVFHVETGALRGRLSE
                     LKTWGTVNRASVLLVRAKVTKGRLDFGEQTPAFQGINGELTLTEREFSLQGMEGNFGA
                     APFTLTGKIDNYTLPAPATYPFTAVIRPTTKELAWLFGNKALGTTSFAGQTTLNLTGR
                     GPAAAYEVRGEWELGSASYSYGEWFAKPAGRANSVHFTVKIDGQGMDITPCLFSLPPL
                     EIEATAFYPFAASTRSVRFTAQSNRVTLPELKVLSPVLTRYEAAGFAQGKIRFNTKSG
                     KSAGGLWQGSLSLQEAAFKPSARLHRLQDLTGIIQWEGDTLTTSRLAGRYGKTPFSLA
                     GTRKESPQPVYQGSLAIPALQMEDLGAGKHLAGVPIRDLLAHVTYKRDVLSFADLSFR
                     LREGRAAAAGETRFSTGAGPARHHYRFRFEGMPVADLFKEVDKDRLITGTTSGQGELT
                     ATSGNDEELLKSLAGNLRLQVETGTINKFPVLAKIFSLLNVSQILRLRLPDMTAEGMT
                     FRRITTTMAIKNGVASTKDFYLDSDAMNIVGMGKTDLVRRTIDATVGLQPLQTVDKVV
                     SRIPIAGWILTDDDQRLLTVYFEAKGPLDNPTVQTIPLRGLSEETFNMFKRVLKLPKK
                     LITDTGEVRH"
     gene            complement(4768..5481)
                     /locus_tag="PLT63_09245"
     CDS             complement(4768..5481)
                     /locus_tag="PLT63_09245"
                     /inference="COORDINATES: protein motif:HMM:NF022694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HOD98947.1"
                     /translation="MFAKRLLSIIIPAALLILLAAGEIHSIPEQSVVVAKYRSPCLVN
                     GVPEGWKLMRHSGTPNINLIREGDAYYVKLKSDPRSGFGIERQVRVDLKEYPYLNWTW
                     RANRLPRGGDVRKSVTDDQVLQIYVVLPATGFPQQVNTPILTYIWDNEAPKGLSVRSP
                     KPLLRKIRYLVVRNKTDHLGSWYTEKRNVYEDYKRIFRDVQGGEPRGATHGVRLYINS
                     QNTKSYAEGCIGEIYFSKN"
     gene            complement(5546..>5800)
                     /locus_tag="PLT63_09250"
     CDS             complement(5546..>5800)
                     /locus_tag="PLT63_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOD98948.1"
                     /translation="HGTKSALEGDVKAGERVVVLDDVITTGGSTITAIERVRAAGLVV
                     DRVIALIDREEGGRENILSYIPRVESVVTRSEIMQVYEKK"
BASE COUNT         1073 a         1747 c         1750 g         1230 t
ORIGIN      
        1 cgtacgccca ctagaccctg aacctagcgc gtctaccaat tccgccactt cggcatattt
       61 tatggaggtt gttttctaca cttgtcatcg tctccttgtc aagcaaaatt gatcttgaaa
      121 ataaaaggaa tcatgttatg gtggcgccct atgataacca tccggcgact tgaggatata
      181 tcggcagagc taaaaggctc ttttgtaacc atcggcaact tcgacggcgt tcaccgcgga
      241 caccaatacc tgtttcaaaa tctggtccgg caggcccgca acgccggcgc cggggcggta
      301 gtcattactt tcgatcccca cccgaaaatg gtcctgcacc ccgaacgccg ccccttttat
      361 ctgattacca ccctggagga gaagatcaag ctcatcgcca cccaaggggt ggacgggttg
      421 ctcgtcatcc ccttttcccc ggaattttcg cggaccacgg cccaggaatt cgtctgcgat
      481 ctcctctggg acaaactgag gatcaggaag atccttatcg gccacgatta tacctttggc
      541 cggggaaagg agggcaacga ggcctttctc gtcgctcggg gggagcattt gggcttcgcc
      601 gtggaggtga tgaacgcctt caccagcaac ggggacatca tcagcagcac tttggtgcgc
      661 aacgccatcc tggccggcga tgtaaaaaag gcggcggcct gtctgggccg tccgtacaat
      721 ctggtgggcg tcgttgtcgc cggtcatcgc cggggggccg gtctgggttt ccccacggcc
      781 aatatccaac cggagaaggt tctcctgccc gcccggggcg tctatgcggc cctgaccaag
      841 ctggccggcc ggacctaccg cagcgtccta aatatcggcc tgaatcccac cttcgccgat
      901 gagcagctct ccattgaagt gcacctcctc gattttaatg aagatattta cggccggacc
      961 ctggaggtac tgttttggga acaattacgg gaggaagtga agttcaacgg acccgccgcg
     1021 ttgattgccc agatcgagcg tgatgtcgcc cgggctcggg agctcctgcc ttgaagataa
     1081 ggccgctgca ttcctgggat atcccccggg gaaacgccgt cgccctccaa aacaacctcc
     1141 gaacacagct tgtcttggcc gatcccgatc cggcggagga taggataacc tgcatcgccg
     1201 gggccgacat ctcctatgcc cgtcacagcg acctgtttta tgccgtcgtg gtcctgcttt
     1261 cctaccccgg cctggcggtc cgggaagagg cccgggcgat cggccgggta tctttcccct
     1321 atgtgccggg cttactcagc tttcgggaag ggccgatcct cttgcaagcc atggaaaagc
     1381 tgtccgtcgt cccggatgtg gtcatgttcg acggccacgg catcgcccat ccccggggct
     1441 ttgggctggc gtcccacctg ggactgtggc tcgatctgcc gtcgatcggt tgcgccaagt
     1501 cccggctggt gggcgactac cgggagcctg ggccggatgc gggggaggaa gagcccctcg
     1561 ttttcaaggg aaagattatc ggcatggtgt tgcggacgaa gcggcgggtg cgcccggtct
     1621 tcatctcccc tggccaccgg atcagccttg ctcgggcccg ggatgtcgcc cgctggtgct
     1681 gctgcggtta tcgcatcccc gaacccgtac ggcaggccca tctggccgta aaccgcttgc
     1741 gcttggctgg cgggatcgct aatggcgaac ttcgccggtg tcggtgatga gttttttcgg
     1801 gagcttcaat acccgtttga acatattgaa ggtctcctcc gacaaccccc ggaggggtat
     1861 ggtctggacg gtgggattgt ccagcggtcc cttggcctcg aaatatacgg tgaggaggcg
     1921 ctggtcgtca tcggtgagaa tccatccggc gatgggaatc cggctgacca ccttatcgac
     1981 ggtctgcaat ggctgcaggc cgacggtggc atcgatggtg cgcctcacga ggtccgtttt
     2041 tcccatgccc acgatgttca tggcgtcgct atccagatag aaatccttgg tgctcgccac
     2101 gccgtttttg atggccatcg tggtggtgat gcgtcggaaa gtcatccctt ccgccgtcat
     2161 gtccgggaga cggagccgga ggatctgaga tacattgagg agggaaaaga tcttggccag
     2221 gaccgggaat ttattgatgg tgcccgtttc gacctgcaaa cggaggttcc ccgccaatga
     2281 tttcagcagc tcctcgtcgt tgccgctggt ggctgtcaac tccccctggc cggaagtggt
     2341 cccggtgatc agccggtctt tgtccacttc cttgaagagg tccgccacag gcatgccctc
     2401 gaaccggaac cggtaatgat gacgcgccgg accagcgccg gtggagaagc gtgtttcacc
     2461 cgcggcagca gcgcgccctt cccgcagacg gaaggaaagg tcggcaaaac tcagcacatc
     2521 tcgtttgtag gtaacatggg ccagcaaatc ccggatcgga acgccggcca ggtgtttgcc
     2581 agcccccaaa tcctccatct ggagggcggg gatcgccagg ctcccctgat agacgggttg
     2641 aggtgattcc ttcctcgttc cggcgagcga gaagggcgtc ttgccgtacc tccccgcgag
     2701 gcgggaggtg gtgagcgtgt ccccctccca ttggatgatc ccggtgagat cctgaagccg
     2761 gtggagacgc gccgacggtt tgaaggccgc ctcctgtaag gaaagactgc cctgccaaag
     2821 accgccggcc gacttgccgc tcttcgtatt gaatctgatt ttcccctggg cgaatccggc
     2881 ggcctcgtac ctggtcagaa cgggggacag aacctttaac tccggtaagg tcacacgatt
     2941 ggattgcgcg gtaaacctga cgctccgggt ggaggcggca aagggataaa aggccgtggc
     3001 ctcgatctcg agggggggga gagaaaagag gcaaggggtg atgtccattc cctgtccatc
     3061 tatttttacc gtgaaatgga ccgagttggc ccggccggct ggtttagcga accactcccc
     3121 gtagctgtat gaggccgacc ccagctccca ctccccgcgc acttcgtaag ccgccgccgg
     3181 tccccggccg gttaagttca gcgttgtctg gccggcgaag gaggttgttc ccagcgcctt
     3241 gttcccgaaa agccaggcca gctccttagt ggtgggccgg atgacggcgg tgaagggata
     3301 ggtagccggc gcgggcaggg tgtaattatc gatctttccg gtgagtgtaa agggagcggc
     3361 gccgaaattt ccttccatgc cctgaaggga gaactcccgt tccgtcaggg tcaactcccc
     3421 gttgatccct tggaaagcgg gcgtctgctc cccgaaatcg aggcggccct tcgttacctt
     3481 cgccctgacg agaaggacgc ttgcccggtt aactgtaccc catgtcttca gctcgctcag
     3541 ccgccccctg agggcgcctg tctccacatg aaataccccc gcccggacat atttgcggat
     3601 gaagttcccg gtggaggcgg gaatgattcc gtaaggaatc aaattcccgt gcctttccag
     3661 gtcgaagtcc gccacggagg tcgtcgcctc cacaaacgga tccggggaaa ggagatcttt
     3721 gagggtcatt tttccctgca ccttaaggcc gtccagggcg gcttccgcac ggctcatgac
     3781 caccgcgccg ggcgtccggt gtacatcgca ggtcaggcgc agcctggccg ccttgatcgt
     3841 ttcgggaaaa acggggggat aggccagctc cggatcagtt atttccaggg acccggaagc
     3901 agtgaaatcg tggcccgggc cgttcaggac gagctcggcg gtcaggaggc cgccgagttt
     3961 ctggaagggg gtccaccggc gataccacgg ccagggaccg gccagatcga gttgttccgt
     4021 acgcaacttt gtcctgatca gggagctgtc caatatgtcc tcagtcttct tcgccaaccg
     4081 cacctttccc gccagggaca gacggccgtc cccgcttttt cccgccagcg tgccggcgag
     4141 ggaaaaagcg gccgtcgccc cccgttccca gcgctccaga tgaaaatcga tggccttgat
     4201 gaccaggacg ccttccttta tcgtcgtatc gacgagggtg atttcacccc cggccgcggt
     4261 tatccccccc acatccacct ccggagcctc ggtggcggta aacaggtcgg cgatgttcca
     4321 ccttccctcc cgatcccgga caatccgcat ccggggttct tccaggcgga tttcccggac
     4381 cacgagcctc ttttccagga tatagggtaa aacggcgatg cggacggcga tttccgccgc
     4441 cgaagcgaag ggctcggggc cactcttctc ggcgattttg acaccggtga aggtcagggc
     4501 cggtccgaag cggaaggaaa gacgggcgga ttcatattgc aggtcccgtc ccagggcccg
     4561 gcgggcgtag gcctctatgg tctgccggta cgcctccagg ttctgcaact gcctgaagac
     4621 atggaggccc acagcggccg cggcgacggc aaaggcgagg agcatcccga tgatcagaac
     4681 ggtttttttc ttcgtgaccg gaaacaattc cgaaacctcc acatcgtggc gaggggaaga
     4741 caatgggaac aaccggctcc gtcagggtca gtttttactg aagtagattt ccccgatgca
     4801 gccttcggcg tagcttttgg tattctgtga attgatataa agccggacgc cgtgggtggc
     4861 cccccggggc tccccacctt ggacgtcccg gaaaatacgt ttatagtcct cgtaaacatt
     4921 gcgtttttcc gtgtaccagc ttcccagatg gtcggtcttg ttgcgcacga cgagatagcg
     4981 gatctttctc agcaggggtt tgggactacg gacggacagt cccttcgggg cttcgttgtc
     5041 ccagatatag gtaaggatcg gtgtgtttac ctgttgcggg aagcctgtcg ccggcaggac
     5101 cacgtagatc tgcagcacct ggtcatccgt gacggatttg cggacatcgc cgccccgggg
     5161 gagccggttg gcccgccagg tccaattcag atagggatac tccttgagat ccacccgcac
     5221 ctgcctctcg atgccgaaac cggagcgggg gtcgctcttc agctttacgt aataggcatc
     5281 gccttcccgg atcagattga tgttcggggt gccgctgtgt ctcatgagct tccagccttc
     5341 gggcacgccg ttgaccagac agggggaacg gtatttcgcc accaccacgg attgttccgg
     5401 aatggaatgg atctctcccg ctgccagcag gatcagcagc gctgccggga tgattatgga
     5461 caaaaggcgt ttcgcgaaca tctctcctct gtttaggatg cagtttcggc ggtgccttta
     5521 ccgtagcgcc ggccgtttta gcaagctatt ttttttcgta aacctgcatg atttcagagc
     5581 gggtcaccac ggattccacc cgggggatgt aggacagaat gttttccctc cccccttctt
     5641 cgcggtcgat cagggcgatg acccggtcga ctaccagtcc cgccgcccgg acccgctcga
     5701 tggccgtgat cgtcgagccg ccggtggtga tgacgtcgtc gaggacgacc acccgctcgc
     5761 cggcttttac gtccccttcc agggcgcttt tggtcccgtg
//
LOCUS       DAOSMF010000117         5747 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_15306_length_5747_cov_21.822558, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000117 DAOSMF010000000
VERSION     DAOSMF010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5747
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15306_length_5747_cov_21.822558"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="PLT63_09255"
     CDS             complement(<1..398)
                     /locus_tag="PLT63_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98949.1"
                     /translation="MKKLITLAQISCDAQGFQSHIERIKAVIAQYRASDLIVFPELIL
                     HGHPSRERPEGFLFRRSEGRERRLSEDMAAYVRELDARIIIGEIRRKGDTFYNLATYM
                     DYATVQSYAKVHVHWTEQFVPGRDFPVLSTP"
     gene            complement(414..2036)
                     /locus_tag="PLT63_09260"
     CDS             complement(414..2036)
                     /locus_tag="PLT63_09260"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HOD98950.1"
                     /translation="MCTICGHFSHGEAIAATDIYDMLKKMAHRGPDMHGVYLDGVVTR
                     RKTVEEFKTLLKRKIRIALGHSRLGIIGSEDLSQPYTSCDGKLTLIKNGEIYNHEALK
                     SLLLYSHHHGTTSDSEILVHLLEESYRGDLLEAVKRVIGIIDGMYAFTVTDGESVVLA
                     RDPIGKKPLYYVESGPVTYFASGKKALWNGTEEPVRLRPGEIVCIDKTGRSNHTGVAI
                     QMPQIDIVDFRDAVERYKIVLVKAMQKRLAGLREDCLGVIFSGGIDSVLIAKLLQREG
                     KNITCYCTGTADSGDILAAQTVAGQLGLELKTAIIDADLVQGILSEVIRNVEEGGLLQ
                     VEVAIPMYMAAKMAAEDGIRVMYTGQAADELFAGYPWYSDVLKEWGYLRLHEQLWEDL
                     NYLYDDTLEREDKLTMSHSIELRAPYLDRDVIRTAMRISPRLKIQGSSDELRKRVHRQ
                     AAVELGVPPFLAFRVKDPAQSGSGIHQIIETIARTQNVALDKELVTENIKIDKGSLYR
                     YGRGVYGEEASRDYIQRLHREIQNSYKPAILE"
     gene            complement(2146..3141)
                     /locus_tag="PLT63_09265"
     CDS             complement(2146..3141)
                     /locus_tag="PLT63_09265"
                     /inference="COORDINATES: protein motif:HMM:NF014027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HOD98951.1"
                     /translation="MKKTNSYLSVNEFITHHFRHFNASELKRASEAYVSHLQREGKMF
                     LAMAGAMSTAELGLSLADMIRNDKIHAISCTGANLEEDIFNLIAQKDYVPVPNYRSLS
                     AEDEQRLFERKLNRVTDVCIPEVAAMRRLEECVLQLWKQAEAEGKRYFPYEYMYALIR
                     GDVLRESCQIDPRDSWLVAASEKDIPIFVAGWEDSTLGNVFAASYLQGKIRRLDIVKT
                     GMEALLSLVAWYKERSARHEIGFFQIGGGIAGDFSICVVPLIQQDLNEDCRLWSYYCQ
                     ISDSTTSYGSYSGAVPNEKITWGKLSPDTPSFIIESDATIVAPLIFAYVLGADGE"
     gene            complement(3501..5630)
                     /locus_tag="PLT63_09270"
     CDS             complement(3501..5630)
                     /locus_tag="PLT63_09270"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOD98952.1"
                     /translation="MNRLMLQTKLIGFLLLTSAVIIVFSLYLLIDAWRANDRAEAIIE
                     LNRQADFLFMSLKDLTFERGRTNVVLSSGKPVSSSDREFIETRRKSVDEKITRGLAWL
                     EKEDPPLADRLRGDYKNMLRLRQEVDSVVSGNATAERLRLAEVWYEQTTGIIHYIIKV
                     LEIIGKRQEAPRRFGIYQRLLIDTLVFRDRIGQSGSIMTAALAKNLPLSAMQYRRFTE
                     NLAQADYVWSEIEAESVILRDEKLDQQKAVVYKEYYGKYRPVLEENIPTALAGKSSPE
                     KIRQLKELSVTVFDSVFVLLHQYKDAVATEMDEQKNKAFISLVLAMIQFIIALTIVAA
                     TIFYFRSRLFQPLDHLIRALGSIQRGENVSDLSAEMTRADEIGQLAAGVKMLEKSMAE
                     ERILRKLTEHRAVRDELTGLHNRHFLEMNLESVMNRSDRYDEYVSMVMFDLDFFKRVN
                     DSWGHPAGDAVLRQTARLAEQLVRGSDWLIRFGGEEFLVLMPQTTIEGAVVAAEKLRN
                     SFGRHNHPGIGIVTASFGASQRKKNESFSAWYARTDAALYEAKQTGRNRVVSATEVDL
                     PVRAAQVTWLAQWETGHSLIDGQHKKLVELSNSLIAASISPELEPEKVLTCLNELLDC
                     LVRHFESEEKILKEAGYPEAADHAARHFELLREIDQLKASYLEKEIKDSAFFLFVVND
                     VVMEHMLKEDKLFYPYIPPSGNHGVTC"
BASE COUNT         1198 a         1629 c         1570 g         1350 t
ORIGIN      
        1 ggggttgaca ggacgggaaa atcgcggccg ggaacgaact gctccgtcca gtggacatgg
       61 accttggcgt aactctggac cgtggcgtag tccatatagg tcgccaggtt gtaaaacgta
      121 tcccccttgc gccggatctc cccgataatg atccgggcgt ccagttcgcg gacataagcg
      181 gccatgtctt ccgaaagacg tcgctctcgc ccttcgctac ggcggaaaag aaatccttcc
      241 ggccgttccc gggaagggtg gccgtggagg atcagttcgg gaaagacgat gagatcggaa
      301 gcacggtatt gggcgatgac ggccttgatc cgctcgatat ggctctggaa gccctgggcg
      361 tcacagctta tctgggccag ggtaatgagc tttttcattg cataaccccc gtttcattcg
      421 agaattgccg gtttgtagga attctgtatt tcccggtgga ggcgctggat atagtcccgg
      481 gacgcctcct ctccataaac cccccgcccg taacggtaca ggctgccctt gtcgatcttg
      541 atgttttccg tgaccagctc cttatccaag gcaacgttct gcgtccgggc gattgtttcg
      601 atgatctggt ggatccccga accggattgg gcgggatcct tgacgcggaa ggccaggaag
      661 gggggaaccc ccagctccac ggcggcctgg cggtggaccc gcttgcgaag ctcgtcggag
      721 ctgccctgga tcttcagacg cggggagatg cgcatggccg tccgaatgac atcccggtcg
      781 agataggggg cccgcaactc gatggaatga gacatggtca gtttgtcttc ccgctccagg
      841 gtgtcgtcgt agaggtagtt gagatcctcc cagagctgct cgtggaggcg cagatagccc
      901 cattccttca agacgtcgct gtaccacgga tacccggcga aaagctcgtc cgccgcctgg
      961 ccggtgtaca tgacgcgaat cccgtcctcc gcggccatct tcgccgccat gtacatgggg
     1021 atggccacct ccacctggag gaggcccccc tcttccacgt tccggatcac ctcggagagg
     1081 atgccctgga ccaagtccgc gtcgatgatg gcggtcttga gctccagccc gagttgcccc
     1141 gccacggtct gggccgccag gatgtccccc gaatcggcgg tgccggtaca gtagcaggtg
     1201 atatttttcc cttcccgctg gagcagcttg gcgatcagga ccgagtctat ccccccggaa
     1261 aaaatcaccc cgagacaatc ttcccggagg ccggccaacc gcttttgcat ggcctttacc
     1321 aggacgatct tgtatctttc cacggcgtcg cgaaaatcca cgatgtctat ttggggcatt
     1381 tggatcgcca cgccggtgtg gttggaccgc ccggttttgt cgatgcagac gatttccccg
     1441 ggacgaagcc ttacaggctc ttccgttccg ttccagaggg ccttttttcc cgaagcgaag
     1501 taagtgacgg gaccgctttc cacataatac aaaggtttct tgccgatggg gtcccgggca
     1561 aggacgacgg attcaccgtc cgtgacggtg aaggcgtaca tgccgtcaat gatccctatc
     1621 acccgcttca ccgcttccag gaggtcgccg cgataagact cctcgaggag gtggaccaat
     1681 atctcgctgt cgctggttgt tccatggtga tggctgtaca gcagcagcga tttgagggct
     1741 tcgtggttgt agatctcccc gttcttgatc agggtaagtt taccatcgca ggaggtgtag
     1801 ggctgggata gatcctccga gccgatgatt cccaggcggg agtgccccaa ggcgatccgg
     1861 attttccgct tcagcagtgt cttgaattcc tcgacggttt tgcgcctcgt cacgacgccg
     1921 tccagataga cgccgtgcat gtccggaccc cggtgcgcca tttttttcaa catgtcgtaa
     1981 atgtccgttg ccgctatggc ttccccatgg gaaaaatgac cgcagatggt acacatggat
     2041 ttgccctcct ttgggttttg tcgctccctg tttgaatagt cgcggagatg aaaacgctaa
     2101 gggtaaacat aaatatatcg acggtatcga gagccctcgt ccccttcact cgccgtcggc
     2161 gcccaggaca taggcgaaga tcaggggggc gacgatggtg gcgtcgctct cgatgatgaa
     2221 actgggggtg tccggcgaca gctttcccca ggtgatcttt tcattgggga cggcgccgct
     2281 gtaggaaccg taagaggtgg tactgtcgga tatctggcag tagtagctcc ataatcggca
     2341 gtcttcattg agatcctgct ggatcagcgg gacgacgcag atggagaagt cgccagcaat
     2401 gccgccgcca atctggaaga agccgatctc atgccgggct gaacgctcct tgtaccaggc
     2461 caccagggac agcagcgcct ccattccggt tttcacgatg tccaaacgtc ggatttttcc
     2521 ctgaagataa ctggcggcaa agacattccc cagcgtcgag tcctcccagc cggcaacgaa
     2581 aatcgggatg tccttttccg aggcggcaac cagccaggag tcacgagggt cgatctggca
     2641 gctttcccgg aggacgtcgc cgcggatcag ggcgtacatg tattcgtagg ggaaatagcg
     2701 ttttccctcc gcttcggcct gcttccagag ctgaagaacg cattcctcca accgtcgcat
     2761 ggccgcgacc tcggggatgc agacatcggt gacgcggttg agcttcctct cgaaaagccg
     2821 ctgctcgtcc tccgccgaca gggaccggta gttgggcacg gggacatagt ccttttgggc
     2881 gatgagattg aagatatcct cctcgagatt cgcccccgtg caggaaattg cgtggatctt
     2941 gtcgttacgg atcatatcgg cgaggctgag ccccaactcc gccgtgctca tggccccggc
     3001 catggcgaga aacatcttgc cttcccgttg caggtgggag acataggctt ccgatgcccg
     3061 tttgagttcc gaggcgttga agtggcggaa gtgatgggtg atgaattcgt ttaccgatag
     3121 ataggagttt gttttcttca tgtcaccttc gctatggttt attcccttgg cgggtgtgtc
     3181 gaaggcggta tgaatccgga acaatcttca tttgttccgc cttctcagcg ggtggatgaa
     3241 cggttggcgc cgaggttgcc taccggaagt atgcttctcc gagctcggca cataaagcgc
     3301 caagaatcgg gaagtggtct gcgacgtcgg tgtccgggtg aattatcaga ctaacaagta
     3361 gggctgctac atcccataaa taagccccgg tgtcaataaa aaagacccca atatcctttg
     3421 acatgcgaag ttctccgtcc ctatgcttga cgacggcgaa aaaagtcgcg aatgaccctt
     3481 ccctgtcttg ccgcattata tcaacaagtt acaccgtggt ttccggaagg ggggatatag
     3541 gggtaaaaga gtttatcttc cttaagcata tgttccatca ctacatcgtt cacaacaaat
     3601 aaaaagaagg ccgaatcctt gatctctttt tcaagataag acgccttcag ttggtcgatt
     3661 tctcgcagca attcaaagtg ccgcgccgcg tgatccgcag cctccgggta gcctgcctcc
     3721 ttcagaattt tttcttcgct ctcaaaatgc cttaccaggc aatccagcag ctcgttcaaa
     3781 cacgtcagca ccttttccgg ttccagctca ggcgaaattg acgccgcgat caggctgttc
     3841 gacagttcca ccagtttctt gtgctgccca tcgatcaatg aatgtccggt ttcccattgg
     3901 gcgagccatg ttacttgcgc cgcccggacg ggcaagtcga cttccgtcgc actgaccacg
     3961 cgatttcgcc cggtctgttt cgcttcatac agggccgcgt cagttctcgc ataccaagca
     4021 ctgaacgatt catttttctt cctctgcgaa gcgccgaagc ttgccgtaac aatgccgatc
     4081 cccggatgat tgtggcgtcc gaaagaattg cgcagctttt cggcagccac gactgccccc
     4141 tcaatcgtgg tttggggcat cagtacaaga aactcctcgc cgccgaaacg tatcaaccag
     4201 tccgagccgc gaaccagttg ctccgcaaga cgggcggttt gcctgagcac cgcatccccg
     4261 gccggatgcc cccaggagtc gttcacccgt ttaaaaaaat ccaggtcgaa catcaccatc
     4321 gagacgtatt cgtcgtaacg gtcggaacgg ttcatgacgc tttcaagatt catctccagg
     4381 aaatgccggt tgtggaggcc cgtcaattca tctctgacgg cccggtgctc cgtcagtttc
     4441 ctcagtatcc tctcctcggc catgcttttc tccagcattt tgacacccgc ggccagttgt
     4501 ccgatttcat cggcacgggt catttccgcg ctcaagtcgg atacgttttc cccccgttga
     4561 atgcttccca gcgcccggat cagatgatcc agaggctgga ataatctgct gcggaaatag
     4621 aaaatggtcg cggcaacgat tgtcaaggcg atgatgaatt gaatcatggc aaggacaagg
     4681 gaaataaacg ctttgttttt ctgttcatcc atttccgtgg caacggcgtc cttgtattga
     4741 tgaagcagaa cgaataccga atcaaacacc gtaaccgaca gctctttcag ttgcctgatc
     4801 ttctccggcg atgacttccc ggcaagagcc gtcggaatgt tttcctccaa taccggccga
     4861 tatttaccgt aatattcttt gtacacgacg gctttctgtt gatcgagctt ttcgtcgcgg
     4921 agaatgacgg attccgcctc tatttcggac caaacataat cggcctgggc cagattctcc
     4981 gtaaaacgac gatattgcat tgcggacaag ggaagatttt ttgcaagagc ggcggtcatg
     5041 atgctgccgc tctggccgat cctgtcccgg aataccaggg tgtcgatgag caagcgttga
     5101 taaatgccaa aacgacgggg agcctcctgc ctcttgccga taatttccag aactttgatt
     5161 atataatgaa tgatcccggt agtttgttca taccagacct ccgcaaggcg gaggcgctcc
     5221 gccgtggcgt tgccggacac aaccgaatca acttcctgtc tcaggcgcag catattttta
     5281 taatccccgc gcagcctgtc ggccaacggc ggatcttctt tctccagcca ggccagcccc
     5341 cgggtgattt tttcatccac cgacttgcgt cgcgtttcga tgaactcccg gtccgaagat
     5401 gaaaccggtt tccccgacga caggacgaca ttcgttcgtc cccgttcaaa agtcagatcc
     5461 tttaaggaca taaagaggaa atcggcttgc cggttgagct cgatgatggc ctccgccctg
     5521 tcgtttgccc gccaggcgtc gatgagcagg tatagagaga aaacaatgat aaccgcgctg
     5581 gtaagcaaaa ggaaaccgat aagtttggtc tgtaacatca ggcgattcat ttttacacct
     5641 ctcaagtatg taaatatcca cagcggacct gaaggtctgc gcggcatttt catgccgctt
     5701 tgcgtcggct gccgtcatgt catgctttta tcctccgctt cacaagg
//
LOCUS       DAOSMF010000118         5713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_15441_length_5713_cov_13.714917, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000118 DAOSMF010000000
VERSION     DAOSMF010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5713
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15441_length_5713_cov_13.714917"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="PLT63_09275"
     CDS             <1..420
                     /locus_tag="PLT63_09275"
                     /inference="COORDINATES: protein motif:HMM:NF037521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zincin-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HOD98953.1"
                     /translation="PEIRHTTEARAYYRPSTDSVTMPLRESFHSAAEYFSTLYHELTH
                     STGALNRLARKSLADWAPFGTADYSREELVAEMGAGFLCAHAGIESATLENSAAYIRS
                     WLQVLKDDRRAVIVAAGQAQKAADFILGGPAEDEHIN"
     gene            1025..1546
                     /locus_tag="PLT63_09280"
     CDS             1025..1546
                     /locus_tag="PLT63_09280"
                     /inference="COORDINATES: protein motif:HMM:NF015935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HOD98954.1"
                     /translation="MLGGFPRRHDMNKKTDYAEMAKRCIAHLVRESQLSSQPVNNPEE
                     AAQFVALAMVAGGDPCRECVGVLCLNAAGRVTDVRILGVGTVNQAPVYPREVAKAALL
                     SAATAVILFHNHPGGQCRPSAEDLALTRSLGEALRPLDIELHDHLILAGNQVYSTTFG
                     RIVPFAPEARTLF"
     gene            1601..1981
                     /gene="ssb"
                     /locus_tag="PLT63_09285"
     CDS             1601..1981
                     /gene="ssb"
                     /locus_tag="PLT63_09285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOD98955.1"
                     /translation="MVNKVQLIGRLGKDPEVRATQENVAFARFSLATDTHWTDRNGDK
                     QSRAEWHNVLAWGRLAEICGEYLRKGRLVFVEGRLQTRSWEDEAGQRHSRTEVVIHDL
                     KILEPKQPVEAPQPPADNPAEIPF"
     gene            2217..2420
                     /locus_tag="PLT63_09290"
     CDS             2217..2420
                     /locus_tag="PLT63_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98956.1"
                     /translation="MQKESLDNPFCDRERRAIHVPSSALAPFEECAAGSPRLVLLPPV
                     VLDVGPIHRRGSCHPPARDGDAG"
     gene            complement(2756..3370)
                     /locus_tag="PLT63_09295"
     CDS             complement(2756..3370)
                     /locus_tag="PLT63_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98957.1"
                     /translation="MRTRMKVISMVVCFFAVCLSVQSGEKNQQQSQRSRHELSEVRKE
                     IRNFFQTWLIQRDTNKAMQYFSSAAFKNTAMLQESFDSNSQMIDRKSENGIKKLIIKF
                     LEAGISGFYPKNLEEAIGSDNLGDLRKQLGCKVLNTNDEFLLIWLLPKEIPEDDETFI
                     RLKEQLPSTGFYVSFAFVRGGLCSFCWIKEKGKWRIFHAEVRGM"
     gene            complement(3386..4168)
                     /locus_tag="PLT63_09300"
                     /pseudo
     CDS             complement(3386..4168)
                     /locus_tag="PLT63_09300"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            complement(4184..4588)
                     /locus_tag="PLT63_09305"
     CDS             complement(4184..4588)
                     /locus_tag="PLT63_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98958.1"
                     /translation="MRILITLCCILSISILGASACQSTPVGKEKIALTQKEKREKIKF
                     LTDEAIKPFIGQTVGVLLDSISLKQEKHIYFQEPPGLWAGCCYFFSNEQGLYVFVDDF
                     QYHDQRDKKTVPNDEAFSKEIITGFRIIEWER"
     gene            complement(4594..>5713)
                     /locus_tag="PLT63_09310"
     CDS             complement(4594..>5713)
                     /locus_tag="PLT63_09310"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HOD98959.1"
                     /translation="YYDADGKRRIKVKTAGGAATEPDGQGGVRLVADRSFYFYEGEDL
                     ICQQDVGALVARIPGTDDIDESVYGPKFLLLDHLGSTRAELVFTESGGVFSPVIQEYY
                     DLMPYGEVITPPSTQESVLFTGKFRDTESGLDYFGPRGFNPNLYRWLSPDQPFADSMT
                     DNPQSWNLYAYCRGNPIVRIDITGFDIVELTKIVRQISSDLNQAYSSSFRLYQRRSGE
                     IIPDSVEVGGNIVRTSKGKFTTRDIHDDNKRTEVSIRPDRIESGDALVGHFHTHRVEP
                     GSTFSSSDIGNADEAGKYSILESGTKRFAMEITDIKAFTSFMKQNEGAILDALQKAQV
                     KSKEDEVKVIANFANQKGSGFKLYQTVDDKKVKWEEVK"
BASE COUNT         1198 a         1721 c         1465 g         1329 t
ORIGIN      
        1 ccggagatcc gccacaccac ggaagcccgg gcctactacc ggccgtccac cgacagcgtg
       61 accatgccgc tccgggagtc cttccactcg gccgctgagt atttttccac gctctaccac
      121 gaactaaccc atagcacagg agccctgaac cgcctggccc gaaaatctct ggccgactgg
      181 gcgcccttcg gcacggccga ctacagccgc gaggagctgg tggccgagat gggcgccggg
      241 ttcctgtgcg cccacgccgg catcgagagc gccaccctgg agaactccgc cgcctacatc
      301 cggagctggc tccaggtact caaggacgac cgccgggcgg tgatcgtggc tgccggccag
      361 gcccagaagg cggccgactt catcctgggc ggccccgccg aggacgagca catcaactaa
      421 acccaccggc ccggcccccc cgggccgatt ttcccgccat cgatccataa tctcggccca
      481 cccccaaacc cctgccctag gcgggggctt tccttttcat ccagaccagc cggcctctgg
      541 cggccggggc gccgcccctg ggccccgccg agggaatgcg atcccctcgg gctccccgct
      601 cgattttcct tgattcagcc gagtgggcag cagacacaga gaaggggcgg cggtcgtccg
      661 ttcaccggtg gccggccgca tcgctcccgc caatgccggc ggtcgccttt cgcttggcag
      721 tcgcgtcccg ctccaagcgg gaccgtcttc gccgggtccg ggcgggcctg gccgggtgac
      781 ggcggcccgc aagccgcccg ccaccgcttg cgcgtccctc cgcgctcggc cgagcataaa
      841 cggccgccgc ccccgatcgg ggtcccctcc aattatccct cggcctcgcc gctgcgtgac
      901 gcggcggcgt caggtccgct cccgccggct tgctaccggg ccgcttcgtc tcccggcccg
      961 gcgtctggag gtcattttcc cgcctggccc ttcagcttgg agtgaaaggg ccaggcggaa
     1021 aaatttgctt ggcgggttcc caaggaggca cgacatgaac aagaagaccg attacgcgga
     1081 aatggcgaag cgttgcatcg cccacctggt ccgggagagc cagctctcca gccagccggt
     1141 gaacaacccg gaggaggcgg cccagttcgt ggccctggcc atggtggccg gcggcgatcc
     1201 gtgccgggag tgcgtcgggg tcctttgctt gaacgcagcc ggtcgggtga cggacgtccg
     1261 gatcctgggc gtcggcacgg tgaaccaggc gccggtctac ccccgcgagg tggccaaggc
     1321 ggcgttgttg tcggccgcaa cggccgtgat cctcttccac aaccaccccg ggggccagtg
     1381 ccgcccctcg gccgaggacc tggccctgac ccggagcctc ggcgaggcgc tgcggccgct
     1441 ggacatcgag ctccacgacc acctgatctt ggccggaaac caggtctact ccaccacatt
     1501 cggccggatc gtcccgtttg caccggaggc ccggaccctt ttctaactct gatcaactcg
     1561 ggggccgcgc atccatgcca cgcgggaagg agatttcaac atggtgaaca aggttcaact
     1621 catcggccga ctgggcaagg acccggaagt ccgcgccacc caggaaaacg tggccttcgc
     1681 ccgcttctcg ttggccaccg acacccactg gaccgaccgc aacggcgaca agcagagccg
     1741 cgccgagtgg cacaacgtcc tggcctgggg gcgcctggcg gagatctgcg gcgagtacct
     1801 gcgcaagggc cgcctggtgt tcgtggaggg gcgcctgcag acccgctcct gggaggacga
     1861 ggccggccag cgccacagcc ggaccgaggt ggtgatccac gacctcaaga tcctggagcc
     1921 caagcagccg gtggaggcgc cccagccccc ggcggacaac cccgccgaga tcccgttcta
     1981 aggcgtcata atcggaaccg aaaagcccgg agcgatccgg gcttttcggt ttcttgcgca
     2041 cggccgctgc ggcgaggccc acatctgccc tatatgccac gatctcgccc ggcccctggc
     2101 ccggagtacc ccttcttccg accgggagcg ctgccccctg gtcccccgcc gagggaatgc
     2161 gatcccttcg ggctccccgg attttttctc agtaacaatt catcaggaag tttcggttgc
     2221 aaaaggaatc actggataat ccgttctgcg atcgagagcg ccgcgccatt cacgttccgt
     2281 cgtccgccct cgcccccttc gaggagtgcg ccgccggatc accccgcctg gtcctcctcc
     2341 cgccggtcgt cctcgatgtc gggccgatcc atcgacgcgg ttcctgccac ccacccgccc
     2401 gcgatggcga cgccggataa aaccggaatc cggagagggg ggaagccgaa gattcactcg
     2461 aatgcaggat acgcatttgt ccggacaagc gccgcttgtc cgccccaatg ccggccgccc
     2521 ttggcggccg catgtactga tgttggcact acattttgcc gttcagggaa atccacttct
     2581 cgatttgaca agggtttcaa cagcgtgcag gttaccagtt acactcgaaa ctgtcgaaga
     2641 tgagttgaaa aatagatttg tctatttcaa acaatggggg ttcagactct tttcgatagt
     2701 ctatgccgtt taagatccag ttgaacattg attcagctgg agcttctgag ttgttttaca
     2761 ttcctctgac ttcagcatga aaaatacgcc atttgccttt ctcttttatc cagcaaaaag
     2821 agcagagacc acctctgacg aatgcaaaag acacataaaa acctgtcgat ggaagttgtt
     2881 cttttaacct aataaacgtc tcgtcatctt ctgggatttc tttaggcaac agccatatca
     2941 gcaaaaactc atcgttggta ttaagaactt tacaccctaa ttgcttccgc aaatcaccta
     3001 aattatccga accaattgcc tcctcgagat tttttggata aaaaccacta attcctgcct
     3061 ccagaaattt aataataagc tttttaatgc cgttttctga tttcctgtca atcatttggg
     3121 aattactatc aaaactttcc tgaagcattg cagtattctt aaaagctgcg gaggaaaaat
     3181 actgcattgc tttatttgta tctctctgaa ttagccacgt ttggaaaaag tttcggatct
     3241 cttttctgac ctctgataat tcatgtcttg agcgttggct ttgttgttga tttttctccc
     3301 cagactgcac actcaagcat actgcaaaga aacacaccac catactaata accttcattc
     3361 tggttcgcat gtctataatc ctctcttatt tatcccacca atttctcaat ccaggccagc
     3421 gatctatgaa tttctgataa tttcctggat acctcacatc ggaattaaat ggtttggtat
     3481 gccttctatc tgtcctcgga aaataatcaa tatcaatgtc agatttgctc ttgtccttag
     3541 aataagaagg ctgcaaccct gcctccatcc ccttaatatc cgcatctctt ttctgcctat
     3601 aactgtcggg aaaaccttta tggagaaatt gtattcctaa aaaattgttt gaactatatt
     3661 cgaatcctgg tgttgaagca aatgcctcta tgcctcctgg tttcatgtcc gcatacaatc
     3721 tgaactgctt gtcgcctcct acaccaggga cttctcctgc gatttctgtc actccttgaa
     3781 cttgttgaag agcatttccc aatgaattgc cttgttgatc aaccagttga gtatgttcga
     3841 gcgcataggt aaccgattca aaggttgaca tctgagactc gctgagtgtg ccgtagactt
     3901 cttccgcgct catcgttgac ttgttgtctt cattgtattg gtcgacatac gattgaaata
     3961 cccggcgctg ttccgcgctc agatccgacc aatcgagacc gttggtatcg atgaaacgca
     4021 acggattgtt aagggtgaag ctgtatcggt tccaactttg aggcaatttg acatcggcac
     4081 ttggcatgta gggatccggg ctcggccacc ggcagagttg gtcgctgtag tacctcgccc
     4141 cgaaaaaggt caagcccgat tcaatgtctt tgggtttgcc ggatcagcgt tcccactcta
     4201 taatcctaaa tccagtaatg atctcctttg aaaatgcttc atcattaggc acggttttct
     4261 tatctcgttg atcatggtat tgaaagtcat caacaaatac gtacagacct tgttcattgc
     4321 taaaaaaata gcaacatccc gcccatagcc cagggggctc ttgaaaatag atatgcttct
     4381 cctgcttcag agaaatagaa tcaagtaata cgccaacggt ctgcccgata aatggcttga
     4441 tagcttcatc ggtcaaaaac ttgatctttt ctctcttctc tttctgcgtc aacgctattt
     4501 tctctttacc aacaggagtc gattgacatg cagaagcccc caggatagaa atagaaagaa
     4561 tgcagcacaa ggtgatcaat attctcattg aactcatttc acctcctccc atttcacctt
     4621 cttgtcatcg acagtttggt aaagtttgaa tccgctccct ttctgattgg caaagtttgc
     4681 gataaccttc acttcatctt cttttgattt gacttgtgct ttttgcaggg cgtcaaggat
     4741 agcaccttca ttttgcttca tgaaggaagt gaacgctttt atgtcagtga tctccatcgc
     4801 aaatctcttg gtgccggatt ccagaataga gtactttcct gcttcatccg cattcccgat
     4861 gtcgctggat gagaatgtcg acccgggttc aactcgatgg gtatgaaaat gaccaacgag
     4921 ggcatctcca ctctcaattc gatcgggacg aatgctgact tcagttcgtt tgttgtcgtc
     4981 atgaatatct ctggtggtga attttccttt ggaggttcgg actatattcc cacccacctc
     5041 gactgagtca gggatgattt caccactccg tctctgatat aacctgaaac tgctgctgta
     5101 cgcttggttt agatcagaag aaatctgtct gacgatcttg gtcagttcga caatatcaaa
     5161 acccgtgatg tcgattcgaa caattgggtt gcctcggcaa taagcataca gattccaact
     5221 ctggggatta tcggtcatac tatcagcaaa tggctggtcc gggctcaacc agcggtaaag
     5281 attcggatta aaacctcgag gtccgaaata atccaatcct gactctgtat ccctgaattt
     5341 gccggtgaac agcacactct cttgcgtcga cggcggggtg atgacttcgc cgtagggcat
     5401 caggtcgtag tactcctgga tgaccgggga gaacacgccg ccggattcgg tgaacaccag
     5461 ctccgcgcgg gtggagccca ggtgatccag caggaggaac ttcggcccgt agacggattc
     5521 gtcgatgtcg tcggtgcccg gaatgcgggc caccagcgca cccacgtcct gctggcagat
     5581 gaggtcctcg ccctcgtaga agtagaagct gcggtcggcg accagccgga cgccgccctg
     5641 gccgtcgggc tcggtggccg cgccgccggc ggtcttcacc ttgatccgcc gcttgccgtc
     5701 cgcgtcgtag tag
//
LOCUS       DAOSMF010000119         5710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_15449_length_5710_cov_20.885057, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000119 DAOSMF010000000
VERSION     DAOSMF010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5710
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15449_length_5710_cov_20.885057"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="PLT63_09315"
     CDS             <1..416
                     /locus_tag="PLT63_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HOD98960.1"
                     /translation="GVIIHALETEMNGEPIEKNLNEINGKKYAVYVGSRVSLAHQVQI
                     HGPAVVLDETFVGMQTLVFKSFVGRGCVVEPGCLLMGVTIADGRYVPAGSVIKTQEQA
                     DALPVITEDYPFRTLNKGVLHVNTALAKGYLAANK"
     gene            complement(769..1728)
                     /locus_tag="PLT63_09320"
     CDS             complement(769..1728)
                     /locus_tag="PLT63_09320"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOD98961.1"
                     /translation="MAGIWIFAEKKELVLELLTPGGELAARQGGKLTIVVPEAEGDPA
                     DYFAAGADEVVLLSPLAEDVTLGAWLPVLAGEIRAADPEAVLLAATSRGKEIAARLAA
                     RLETGLASNCLSLAADADGTLVMERLAYGGAALQRVAVMGRPVMATIPPRTFPPAAAA
                     AIPGREGSVRTLPPPPADTVRVVARKEKERSAQDITAAKTVVCVGRGLEKREDLELAR
                     QLAAALGGEIGCTRPISEELHWLPDNLCIGLSGVQVKPELYLGLGVSGQIQHLTGLRG
                     ARLICAVNRDPNAPIFPAADLGIVGDLYDVTPKIIAEIKKIRG"
     gene            complement(1739..2506)
                     /locus_tag="PLT63_09325"
     CDS             complement(1739..2506)
                     /locus_tag="PLT63_09325"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOD98962.1"
                     /translation="MPKVVACFKWVVDEAYIRKGGGGALDFSAVDYKISEYDRNALEE
                     AARCRGEGGTVTALTAGSPEAAKGLKDALSRGADTAVFASDPAFAALDSSQTAAVLAE
                     VIRNRIPYDLIICGEGSGDQYAQQVGPRLAENLGIPCISYVQKLTVAAEGGRITAERR
                     VEEGVEVVEASFPVLVTVLPEINIPRIPGVKDTLMAGKKPVVTVKNEELPPLGAAKLK
                     TLAMTAARLERNCELFGTAPEDIARFVTALQQKGVSG"
     gene            complement(2595..3734)
                     /locus_tag="PLT63_09330"
     CDS             complement(2595..3734)
                     /locus_tag="PLT63_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOD98963.1"
                     /translation="MEFKLTEEQELIRKNMREFADKYVEPIAAEIDENSRHPAEVFKL
                     LAEGDWMGIPIPVEYGGAGADYLTHILAVEELSRSCASTGFTVSIHVGIACMLVLLNG
                     TEEQKKKYLTPMAKGEKLGAFVLTEPGAGTDVMAATTTAVLDGDAYVLNGTKTFTSNG
                     PTGDFYMVFAWTDKAAGRKGMSCFIIPRGTPGMTPGEHFRKMGLRSSQTSEMVFKDCR
                     VPKENLLGKEGAGLVMAMAGFDHGRVGIAAQSVGIIQAALDESIRYSKERVQFGNPIS
                     RNQAISWMIANMATELQAARFLTYHAAWLKDQKQGFSKEAAMAKLFASEAAMRHTIKA
                     VQIHGGYGYCKGAKVERLFRDAKITEIYEGTSEAQRMVISGNVLR"
     assembly_gap    4048..4147
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4148..4390)
                     /locus_tag="PLT63_09335"
     CDS             complement(<4148..4390)
                     /locus_tag="PLT63_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HOD98964.1"
                     /translation="MTTDALDLKAKLGLDVFKTGGAPHIRIREGREREPRLRKMTAIC
                     PAGLYSEGEGGAITLTVDGCLECGTCRLVCGTEILEW"
     gene            complement(4387..5673)
                     /locus_tag="PLT63_09340"
     CDS             complement(4387..5673)
                     /locus_tag="PLT63_09340"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOD98965.1"
                     /translation="MEKFDVIVVGAGLAGLAAAYTLAGQGVECLVLERGDYPGAKNVT
                     GGRIYVNPVRDLFPELWPDAPLERFIVHEGATLMARERSVTFDYSGDELRAAPHQSYS
                     VLRARFDRWLAERVEEKGAMLLTKTLVTDVVKESGRVAGVVAGGDELHANVVVAADGV
                     LSLLAEKAGLHPPESPQEYAVGVKEVIALPAEKIEDRFQLAGNEGAARLFVGAATAGL
                     FGGGFLYTNRESISLGLVVGVAELMEKGGLQNLPALMEDFKRRPEIAPLIRDGATVEY
                     AAHVIPEGGFRALHPLHGDGILVAGDAAGFALNLGFTVRGMEYALASGYYAAQSILKA
                     LETGDYGAEILAEYGRRLQESFVLKDFQNFQAAPAFVKNPKLFDYYPEFKGRLLRDVY
                     AVPAGPKDRLYPTIRKYLHLKEIWSMFKDFREMTKL"
BASE COUNT          995 a         1867 c         1694 g         1054 t
ORIGIN      
        1 acggggtcat catccatgcc ctggaaacgg agatgaacgg cgagcccatc gagaaaaacc
       61 tcaacgagat caacggcaaa aaatacgccg tttacgtggg cagccgggtg tccctggccc
      121 accaggtgca gatccacggt cccgccgtgg tcctcgacga gaccttcgtc ggcatgcaga
      181 ccctggtctt caaatccttc gtcggccggg gctgcgtcgt cgaaccgggc tgcctcctca
      241 tgggggtcac catcgccgac ggccgctacg tccccgccgg ctcggtgatc aagacccagg
      301 aacaggccga cgccctgccg gtcatcaccg aagactaccc cttccggacc ctgaacaagg
      361 gcgtgcttca cgtcaatacc gccctggcca aaggctatct ggcggccaac aaataacgac
      421 tcatccgaag ataaaaaccg gtcccggaag cctgcccccg cgcgttttcc ggggccggtt
      481 tttccagccc gggccatatt gtcctcaaag gtcctattat aagctgtttt actgtttttt
      541 catccgagcc ggccagcttc accccgccgc ggcttggcga taatctattg ggaatagaaa
      601 gcaagaaaac ccccttccct tcaaggggag ggccggggtg gggatggggt taagcccccg
      661 tcatcacaag ccgatgaccc atccccctct tcacctcccc cttgaagggg gaggaacaag
      721 acttcttctc ttgaacatct cccgcaatgg gaaggacaat tttttctctt agcctcgaat
      781 cttctttatt tccgcgatga ttttgggcgt cacgtcataa agatcgccga cgatgcccag
      841 atccgccgcc ggaaagatcg gggcgttggg gtcccggttc acggcgcaga tcagccgggc
      901 gccccgcagg cccgtcaggt gctggatctg tccggaaacg cccagaccga ggtacagctc
      961 cggcttcacc tggacgccgg acaggccgat gcagagattg tcgggcagcc agtgcaattc
     1021 ctcggagatg gggcgggtgc agccgatctc acctccgagg gcggcggcca actgcctggc
     1081 cagctccaga tcttcccgct tctccaggcc ccggcccacg cagaccaccg tcttggcggc
     1141 ggtaatatcc tgggcgctcc gttccttctc cttccgggcg acgacccgga cggtgtccgc
     1201 cgggggcggc gggagcgtgc ggaccgaacc ttcccggccg ggaatcgccg cggccgcggc
     1261 cggcggaaag gtccgggggg gaattgtcgc catgacgggc cgtcccataa cggccacccg
     1321 ctggagggcc gcgccgccat aggcgaggcg ttccatgact aaggtgccgt cggcgtcggc
     1381 ggccagcgac aggcagttgc tcgccagccc ggtttccagg cgggccgcca ggcgggccgc
     1441 aatttccttt ccccgggacg tggccgccag aagcaccgcc tcgggatccg ccgcccttat
     1501 ctccccagcc agcacgggca gccaggctcc cagtgtcacg tcttcggcaa gggggctcaa
     1561 aagtacaacc tcatcggccc cggcagcgaa ataatcggca ggatcaccct ccgcctctgg
     1621 gacgacgatc gtcagttttc ctccctgccg ggcagccaat tccccgcccg gggtcagcaa
     1681 ttccaataca agttcctttt tttccgcaaa aatccagatg ccggccatgt tcacctcttt
     1741 atccgctcac gcccttctgt tggagcgccg tcacaaaccg ggcgatatcc tccggcgccg
     1801 tcccgaaaag ctcgcagttc ctctccaggc gcgccgccgt catggcgagg gtcttgagtt
     1861 tcgccgctcc caaggggggc agctcctcat ttttcaccgt gacgacgggc ttttttcccg
     1921 ccatgagggt gtccttgacc ccgggaatcc gggggatgtt gatctccggc aggaccgtca
     1981 cgaggacggg aaaggaggcc tcgaccacct cgacgccttc ctcgacccgt cgctccgctg
     2041 taatccgacc cccttcggca gccacggtga gcttctggac ataggagata catgggatgc
     2101 ccaggttctc cgccaggcgg gggccgacct gctgggcata ctggtcgccg gagccctcgc
     2161 cgcagatgat cagatcatag gggatgcggt tgcggatgac ctcggccagg accgccgccg
     2221 tctgggagga atccagggcg gcaaaggcgg gatcgctggc gaaaacggcg gtgtcggcgc
     2281 cccgggaaag ggcgtccttc agccctttgg cggcttcggg actgccggcg gtaagggcgg
     2341 tcaccgtccc tccctcgccc cggcagcggg cggcctcttc gagggcgttg cggtcgtact
     2401 cgctgatctt gtaatcgacg gcggaaaaat ccagcgcccc gccgccgcct ttccggatat
     2461 acgcctcatc gactacccat ttgaaacagg caactacctt gggcatagct caatccttta
     2521 caaattaatg gggacggttc ttaagcatgc gctgaatttt ggaaaaatga gaaccgtccc
     2581 caattttccg tttctcagcg gaggacgttg ccggagataa ccatgcgctg ggcctccgag
     2641 gtcccctcgt agatctcggt gatcttggcg tcccggaaca gccgctccac cttggcaccc
     2701 ttgcagtagc cataaccgcc gtggatctgg acggccttga tcgtgtggcg catcgccgct
     2761 tccgaggcga agagcttcgc catggcagcc tccttgctga agccctgctt ctgatccttc
     2821 agccacgcgg cgtggtaggt gaggaaacgg gcggcctgga gttccgtggc catgttggcg
     2881 atcatccagg agatggcttg attccgggaa atgggattgc cgaactggac ccgctccttg
     2941 gagtagcgga tcgactcgtc cagggcggcc tggatgatcc ccacggactg ggcggcgatc
     3001 ccgacccgac cgtgatcgaa cccggccatg gccatgacca gccccgcccc ctccttgccc
     3061 aggaggttct ccttgggaac gcggcagtcc ttgaagacca tctccgaagt ctgggaggag
     3121 cggagaccca ttttccgaaa gtgttcaccg ggggtcatac cgggggttcc ccgggggatg
     3181 atgaagcagc tcatgccctt gcggcccgcc gctttgtccg tccaggcgaa gaccatgtag
     3241 aagtcgccgg tgggaccgtt ggaggtgaag gtcttggtgc cgttgaggac gtaggcgtcg
     3301 ccatcgagga cggccgtcgt cgtggcggcc atgacatccg tgccggcccc cggctcggtg
     3361 agaacgaaag cccctaactt ctcccccttc gccatcgggg tgaggtactt cttcttctgc
     3421 tcttccgttc cgttgaggag gacgagcata caggcgatcc ccacatggat ggagacggtg
     3481 aatcccgtcg aggcgcagga gcgggaaagt tcttccacgg cgaggatatg agtcagataa
     3541 tcggcgccgg ccccgccgta ttccacgggg atgggaatcc ccatccagtc cccttcagcc
     3601 aggagtttga atacctcagc cggatgacgg ctgttttcat cgatctccgc ggcgatgggt
     3661 tccacgtatt tgtcggcaaa ttcccgcatg ttcttgcgga tgagttcctg ttcctccgtc
     3721 agcttgaatt ccataattcc cctcctcgtt catatttttc aagaccgcaa gcggcccgtg
     3781 attacctttg tacgtttgtt ctttccatcg ccggacccgc ccgcccgcgc cgacgggcaa
     3841 cgaaaaatgc tcccaactaa cttcagcgcc gccccttggc aagttccttt cccatacagg
     3901 cccgaacgct cgcaggctgg aacgctaaga aacaagacct gaccccacta tccgcagcgg
     3961 aactggaccc ccgtgccgcc ctccgggtag tcccattcca ggatctcggt cccgcagacc
     4021 aggcggcagg tcccgcattc caggcagnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnncca ttccaggatc tcggtcccgc agaccaggcg gcaggtcccg cattccaggc
     4201 agccgtccac ggtgagggtg attgctcccc cctcgccttc gctgtacaac ccggcggggc
     4261 agatcgccgt catcttccgc aggcgcggct cccgctcccg gccctcccgg atgcggatat
     4321 ggggcgcgcc gccggtcttg aagacatcga gccccagctt ggccttcaga tccagggcgt
     4381 cggtcgtcat agcttcgtca tctcccggaa gtccttgaac atagaccaga tctccttcag
     4441 gtgcagatac ttgcggatcg tggggtagag gcggtccttg ggacccgccg ggacggcata
     4501 gacatcccgg agcagccgtc ccttgaattc cggataataa tcgaagagct tcgggttctt
     4561 cacgaaggcc ggggcggcct ggaaattctg aaagtccttg aggacgaagc tttcctgcag
     4621 gcggcggccg tattccgcca ggatctcggc gccgtagtcc cccgtctcca gggccttgag
     4681 gatcgactgg gcggcgtaat agcccgaagc gagggcgtat tccatccccc ggacggtgaa
     4741 ccccagattg agggcgaaac cggcggcgtc gccggcgaca aggatgccgt caccgtggag
     4801 ggggtgcagt gcccggaatc ccccctcggg gatcacatgg gcggcgtatt ccaccgtggc
     4861 gccatcccgg atcagggggg cgatttccgg acgccgttta aaatcctcca tgagggcggg
     4921 cagattctga aggccgccct tctccatgag ctcggcgacc ccgacgacca ggccgaggct
     4981 gatgctctcc cggttggtat agaggaagcc gccgccgaag agccccgccg tcgccgcccc
     5041 gacgaagagg cgggccgccc cttcgttgcc cgcaagctga aatcggtcct cgatcttctc
     5101 cgccggcagg gcgatgacct ctttgacgcc cacggcatat tcctgaggag attcaggtgg
     5161 atggagcccc gccttctccg ccaggaggga gaggacgccg tcggcggcca ccacgacgtt
     5221 ggcgtgtagt tcgtcgcccc ccgctacgac gccggccacc cggccgctct ccttgacaac
     5281 gtccgtcacc agggtcttgg tcaggagcat ggcccccttt tcctcgaccc gctcggcgag
     5341 ccagcggtcg aaccgggccc ggaggacgct gtagctctgg tggggcgccg cccgcagctc
     5401 gtcgccggag taatcgaagg tcacggaccg ttcccgcgcc atgagggtgg ccccttcgtg
     5461 gacgatgaag cgttccaggg gggcatcggg ccagagttcg gggaagaggt cccggacggg
     5521 atttacgtaa atgcggccgc cggtgacgtt ttttgccccc ggatagtccc cccgctccag
     5581 gacgaggcac tccacgccct ggccggccag ggtataggcg gcagccagtc cggccagccc
     5641 cgcccccacc acaatgacgt caaatttttc catgaccgcc gttctccatc agatcgatcc
     5701 gatttccctc
//
LOCUS       DAOSMF010000120         5705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_15469_length_5705_cov_19.867965, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000120 DAOSMF010000000
VERSION     DAOSMF010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5705
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15469_length_5705_cov_19.867965"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..1607)
                     /locus_tag="PLT63_09345"
     CDS             complement(9..1607)
                     /locus_tag="PLT63_09345"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HOD98966.1"
                     /translation="MIAVIGKILLGLGLMFTGVQMLASGLQQLGGRQFRILATRYVSS
                     RPRALVFGLGSGAVMQSTSAALVILASLISAGMLTVSQAITILTGFSVGNCALVFIVS
                     LDITIGVMFLVGLCGIAMYLIKDDRYRTYLLIGLGLGLIFFGIQMMVAGVKPLRQEAW
                     FTGAMAFSRDHSLLSVMAGVVLGFIAQSSTAVALVAIGLARESILTGPQAFLFMYGAA
                     IGSTIFKVVIGQAFRGTSRQLVRFVNMFNLFGALVFILLYYLEVHGHVPLVMALLDSF
                     LPDLDDQAAWVFLLFNMTSAVFFFAINGPLTRWLARSLPPSAEEGLSHPKYLAEFRQE
                     LPDSGIGLIRLEQTRELEQIAEFVSTARKNYRGANLATRHKAFKALANEVAAATAAIA
                     AMPMQPETAGDHAYTQTRQALLEQLADGTASGVGIIRKASGYPALAGLADACLESIDF
                     LLNYAADTEKTNTPEQLRTLQELSSGNSATMEQLRQAYMDSDRIVSPHDKGCLLDLTV
                     AMEKIIWLINRLISLRQPENEQAG"
     gene            1828..>2221
                     /locus_tag="PLT63_09350"
     CDS             1828..>2221
                     /locus_tag="PLT63_09350"
                     /inference="COORDINATES: protein motif:HMM:NF039822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HOD98967.1"
                     /translation="MKKLVLGMTFFILMFTVVCGADEIRGKVTAPGGDGKSLLISGVT
                     IQAAEAWIEDAEDYPLSLKSIAKGDFVEVDGKFVGPAKMKARKIDRRKLECDAVKGTI
                     TSIDTSQKKIVISGITVKLRADAWLEGPN"
     assembly_gap    2222..2321
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2466..3875
                     /locus_tag="PLT63_09355"
     CDS             2466..3875
                     /locus_tag="PLT63_09355"
                     /inference="COORDINATES: protein motif:HMM:NF014344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HOD98968.1"
                     /translation="MMKKENGKRSSAIKSLCTYSLGLLLALLICGIMGSVSEVAGEAP
                     RECQIAIDGTLPKPGDIAVGSPAEWLAAKTVVVHTPGYEVFLGLIHPTAALFERPFAL
                     DGARREHQAFICLMADNGVKVLRLEDILLAGTMDAAGAMMPGPELDKLRALALSSLSY
                     RATALSSKQAAAQAAYKKQVIATLHPKELLAIILEKPQVILASTHGHNTGLTARYSLQ
                     PVMNLYFMRDQVITTAKGLVIGHFNARQREEETKIVRFAYQKLGIFPVYELKGRARME
                     GGDFIPAGDTALIGQGLRTNAEAVRQLLDNRVFGTPRVAVVKDGWRNQDQMHLDTYFN
                     IIDRNLAVIVEERIDQRDAKGRIIKAADPAKRSLVDLYELKDGRYMKTSGNVPFQQFL
                     EKDLGMTLIPVSNADQLKYGINFLTLRARKIIAIDGVSNDYKERLKASGVDVTWVNFS
                     NLTGGYGAAHCTTQVIHRK"
     gene            complement(3961..4416)
                     /locus_tag="PLT63_09360"
     CDS             complement(3961..4416)
                     /locus_tag="PLT63_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98969.1"
                     /translation="MEKMFGTKMKEFFSSISDGERNNLKQRMEKMAAMCPCMNLVDLG
                     LKEHQILLFDAGISFLFFAQHSLMIRQSFRRFMERFIPESYNNAIYSVSSGIVLAVVL
                     FLWQSSEIKIASVLLTSRSYTGDSGRQNRDPTSEGYIGKTRVCMDRVLP"
     gene            complement(4600..5433)
                     /locus_tag="PLT63_09365"
     CDS             complement(4600..5433)
                     /locus_tag="PLT63_09365"
                     /inference="COORDINATES: protein motif:HMM:NF008823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HOD98970.1"
                     /translation="MNGKDIKEVVREKYGSIAATSGSCCSAASCCGDDSSLVQIGRRI
                     GYSEQDLSSVPEGANLGLGCGNPIALASLKAGETVLDLGSGAGFDCFLAARQVGREGK
                     VIGVDMTPEMIEKATENARKISIDNVEFRLGDIEHLPVADGSIDVIISNCVINLSPNK
                     SRVFREAFRVLKPGGRLMVSDIVLKKPLPDAIKNSIAAYIGCVAGASLREDYLQEMRE
                     AGFESITVQSEAPFPVDCLTTDPMVAGLAESVNLSIEELADHAADLVLSVRVAGLKEG
                     S"
     gene            complement(5457..>5705)
                     /locus_tag="PLT63_09370"
     CDS             complement(5457..>5705)
                     /locus_tag="PLT63_09370"
                     /inference="COORDINATES: protein motif:HMM:NF040962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HgcAB-associated protein"
                     /protein_id="HOD98971.1"
                     /translation="CPASGNANCCRIESLISVDEKGQMVLPKDVRERIGLAPGDKLAL
                     VSWEKDGRICCLALFRADALADQVRGFLEPIMKMSGNS"
BASE COUNT         1352 a         1509 c         1500 g         1244 t
ORIGIN      
        1 tttgcaggct atccggcctg ctcgttctcc ggttgcctga ggctgatcag acgattgatc
       61 agccagatga tcttctccat cgccaccgtc aggtccaaca ggcatccctt gtcatggggc
      121 gaaacgatcc tgtcgctgtc catatatgcc tgtcgcagtt gttccatggt cgcgctgttg
      181 ccggaactaa gttcctgcag cgtccttaac tgctcaggag tatttgtttt ttcggtatcg
      241 gcggcatagt tcagcaggaa gtcgatcgat tccaggcagg catcggcaag acccgccagg
      301 gcaggatatc cgcttgcctt cctgatgatt cccacccccg aagccgtacc gtcggcaagc
      361 tgttccagca gggcctgacg tgtttgagta taggcatgat caccggccgt ctcgggttgc
      421 atgggcatgg cggcaatcgc cgccgttgcc gccgcaacct cgttcgccag ggctttgaat
      481 gccttgtgac gggtggcgag gttggcgccg cgataatttt ttctggccgt agagacgaat
      541 tccgctatct gttccagttc ccgcgtctgt tccagacgga tcagccctat tcccgagtcc
      601 ggcaattcct gtcggaattc cgccaggtat ttggggtggg acagcccctc ctccgccgag
      661 ggcgggagcg atcttgccag ccagcgggtc agaggtccgt tgatggcgaa gaaaaagacg
      721 gccgacgtca tattgaacag caggaagacc caggcggcct gatcgtccag gtccggaagg
      781 aacgaatcca gcagggccat tacgagtgga acgtggccgt ggacctctag gtaatagagc
      841 aggataaaga ccaacgcccc gaagagattg aacatattca caaagcgcac gagctgtcgc
      901 gacgtgccgc gaaacgcctg tccgatcacc accttgaata tggtcgagcc gatcgcggcg
      961 ccgtacatga acaggaatgc ctgcggcccc gtgaggatac tctccctcgc caggccgatc
     1021 gccaccaggg ccacggccgt cgaggactgg gcgatgaatc ccagcactac gccggccatt
     1081 accgaaagca acgaatgatc acggctgaat gccatcgccc cggtaaacca ggcctcctgc
     1141 cgcagcggtt tgaccccggc caccatcatc tggattccga agaagatcaa ccccaggcca
     1201 agcccgatga gaaggtatgt gcggtatcta tcgtctttta tcagatacat ggctattccg
     1261 cagaggccga cgaggaacat tacgccgatg gtgatatcga gggagacgat aaagacgagg
     1321 gcgcagttcc cgacactgaa tccggtcagg atcgtgatcg cctgcgagac ggtgagcatc
     1381 ccggcactga tgaggctggc caggatgacc agggctgccg aggtgctctg catgacggcc
     1441 ccggaaccca ggccaaagac cagggcgcgg ggccgcgatg agacgtagcg ggttgccagg
     1501 atccggaact gccggccgcc caattgctgg agaccggaag cgagcatctg gacgcctgtg
     1561 aacatcagac ccaggccgag tagtatcttg ccgatcaccg cgatcataat caccccagat
     1621 tatccggata attgcctggc cttcattccc ccgccggagc ggttaatgta aacaacaagg
     1681 gtagtgcatg tcaataagat aataaacaag tgaaagtgca aaatcaacgc aagttcggca
     1741 aaaatggatt tgacaaagga tgttatctca tcttaatatt cacccaatgg gatggaaaag
     1801 gcatgttgac acttgaagga gggaatcatg aagaagcttg ttttgggtat gacatttttt
     1861 atcttgatgt tcaccgtggt ttgcggcgcc gacgaaatca ggggtaaagt gaccgccccc
     1921 gggggagatg ggaaaagcct tcttatctca ggggttacga ttcaggccgc cgaggcatgg
     1981 atcgaagacg ccgaggatta tccgctgtcc ctgaagtcca ttgccaaggg agatttcgtg
     2041 gaggtggacg ggaagttcgt cggtccggcc aagatgaagg cgcggaagat cgaccgcaga
     2101 aagctggaat gcgacgccgt caaaggaacc ataacctcga ttgacacatc gcaaaaaaag
     2161 atcgtcatca gcggcattac ggtaaaactc cgggccgacg cctggctgga agggccgaac
     2221 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntcccggaca agcggaagct
     2341 ggtgtccgat ctgagccggt gcactgggaa tccctggaga tatttaatta tattgacaca
     2401 atcactgata aggactactt tcgcctacga aagttgagtg attatcgata aatacgggag
     2461 gccatatgat gaaaaaagag aatggtaaga gatcctcagc gatcaaatcc ttatgcactt
     2521 acagccttgg ccttttgctg gcactgctga tctgtggcat aatgggctcg gttagcgaag
     2581 tagctggcga agcaccacga gaatgtcaga tcgccataga tggcacttta cccaaaccag
     2641 gcgatattgc agttggcagt ccagcggaat ggctcgcggc gaaaaccgtg gtcgtacata
     2701 cgcctggata cgaggtcttc ttaggattga tccatccgac ggcggcgctg ttcgaaaggc
     2761 cctttgctct ggatggagcc cgccgggaac accaggcgtt catctgcctg atggcggaca
     2821 atggtgtcaa ggtattgcgt ctggaggata tcctgctggc gggcaccatg gatgccgctg
     2881 gagcgatgat gccggggccg gagcttgaca agctgcgtgc gctggcattg agttctctca
     2941 gttaccgcgc cacggcgctt tccagcaaac aggccgctgc ccaggccgca tacaaaaagc
     3001 aggtgatagc taccctgcat cctaaagaat tgttggccat cattctcgaa aagcctcagg
     3061 tcatccttgc ctccactcat ggtcacaaca ccggactgac ggcacgctac agcctgcagc
     3121 cggttatgaa cctgtatttt atgcgagatc aggttatcac cacggccaag gggctggtga
     3181 tcgggcattt caatgcccgg caacgcgagg aggagacgaa gatcgtccgt tttgcctatc
     3241 agaagctggg catcttcccc gtctatgaac tcaagggacg ggcccgcatg gaaggcggcg
     3301 acttcattcc cgcgggagat accgccctga tcggtcaggg cctcaggact aatgccgagg
     3361 ccgtcagaca attgctcgat aatcgtgtat tcggcacacc ccgagtggcc gtggtcaagg
     3421 acggatggag aaatcaggac cagatgcacc ttgatactta cttcaatatt atcgaccgca
     3481 acctggccgt gatcgtcgag gagcgcatcg atcaacgcga tgccaaaggg cgtatcatca
     3541 aagctgcgga cccggccaag cggtctttgg tcgatctgta tgaactgaaa gatggcaggt
     3601 acatgaaaac ttcgggcaac gtgccattcc agcaattcct ggaaaaggat ctgggtatga
     3661 cgctgattcc ggtcagcaac gcagaccagc tcaaatatgg aataaacttc ctcacgctaa
     3721 gggcaaggaa aattatcgcc atcgacggtg tgagcaatga ctacaaagaa cgattgaaag
     3781 catcgggagt agatgttacg tgggtaaact tctctaacct gaccggtggt tacggagcgg
     3841 ctcattgcac aactcaggtg atccatagga agtagtggcg aacacttact acgtatgcgg
     3901 tagtgttgag agagggaaga aaagataatg aacatgtgat tcacatatta tctcccatat
     3961 tcagggcagt actcggtcca tacagactcg cgttttgccg atgtaacctt cagaggttgg
     4021 atcacgattt tgccggccag aatcgcccgt atagcttctt gaagtcaaca agacggatgc
     4081 gatcttgatc tccgaactct gccacaggaa aagaactact gccaggacta ttcccgaaga
     4141 aacagagtaa atagcgttgt tgtatgactc cgggataaaa cgctccataa agcggcggaa
     4201 ggattgcctg atcatgaggc tgtgctgagc aaagaacaag aaggagattc ccgcatcgaa
     4261 caagagaatt tgatgctctt tcagtccaag gtcaacgagg ttcatacacg gacacattgc
     4321 agccattttt tccattcgct gcttcagatt atttctttca ccgtccgaaa tgctgctgaa
     4381 gaattccttc attttagttc cgaacatctt ttccatatcc tttctccctc agtacttttt
     4441 tctctttaga cgatgacggc atccaaaagg atacatgcag atctcattag actttcttat
     4501 caacggattt gaattctgat tgccgccgat gatcttttca atgatagccc ttatcgaaca
     4561 ttgcatcgct ttgtcgcaca tttcgtcagg tatcattttt catgatcctt ccttgagccc
     4621 tgcaactctt acacttaaaa caaggtctgc cgcatgatca gccagttcct cgatggaaag
     4681 attaacggat tcagcgagtc ccgccaccat cggatcggtg gtcaggcagt ccacaggaaa
     4741 aggcgcctcg ctctgaaccg taatgctttc gaaaccggcc tcccgcattt cttgcaggta
     4801 atcctcccgg agcgaagctc ccgctacgca tccgatataa gcggcgatgg aatttttgat
     4861 cgcgtccggc aagggcttct taaggacgat atcggagacc atgagtctcc cgcccggttt
     4921 caaaacacgg aacgcctccc ggaagaccct gctcttattg ggggacagat tgataacgca
     4981 gttggaaatg atgacatcga tgctcccgtc tgcgacgggc aggtgctcaa tgtccccaag
     5041 ccggaactcg acattgtcaa tgctgatctt cctggcgttc tccgtcgctt tttcaatcat
     5101 ttccggcgtc atgtccacac cgatgacctt cccttcccga cctacctggc gggcagccaa
     5161 gaagcaatcg aagcctgccc cgctgcccag atcgagaacc gtttcacctg ccttgagcga
     5221 tgcaagggca atgggatttc cacagccaag accaaggtta gccccctcgg gtacggagct
     5281 gagatcttgc tccgaatagc cgatgcgtcg gccaatctgc acgagtgaac tatcatcgcc
     5341 gcaacatgac gccgccgagc agcaggagcc ggatgtcgcg gcaatgcttc catatttttc
     5401 acgaactact tccttgatat cttttccatt cattttttta cctcctgtca attatcttaa
     5461 ctgtttccgc tcatcttcat gatgggttcc aggaatcccc ttacttgatc cgccagggcg
     5521 tcggctcgga agagggcaag gcagcatatc cggccgtctt tttcccagct taccagtgcg
     5581 agtttatccc caggcgccaa cccgattcgc tcccgaacat ccttaggcaa aaccatctgt
     5641 cctttttcgt caacggatat gagtgactcg atgcggcagc aattagcatt tcccgatgca
     5701 ggaca
//
LOCUS       DAOSMF010000121         5608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_15842_length_5608_cov_19.931569, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000121 DAOSMF010000000
VERSION     DAOSMF010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5608
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15842_length_5608_cov_19.931569"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(167..553)
                     /locus_tag="PLT63_09375"
     CDS             complement(167..553)
                     /locus_tag="PLT63_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family EPS-associated transcriptional
                     regulator"
                     /protein_id="HOD98972.1"
                     /translation="MSKPEMPPLNNEDVLKILKAIKEEPALTQRELSSRLGISLGKVN
                     YLIKSLIRRGLVKVDNFKSSSNKISYLYKLTPSGIEEKARTTFYFLKRKLAEYERLER
                     EIVELRNELSGVETHVQGRSDTELRP"
     gene            complement(638..1591)
                     /locus_tag="PLT63_09380"
     CDS             complement(638..1591)
                     /locus_tag="PLT63_09380"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOD98973.1"
                     /translation="MSMPAGGKTPDVSVIVVNWNTGELLKNCLASLRGTRGGPTCEII
                     VVDNASTDGSVAMLRECFPAARVIVNETNRGFGAANNQAFAVMRGRYALLLNTDAVVT
                     EDAVRELFSFMESRPDAAMACGQLLNRDGTKQNSIAAFPTLFTLLFNMPVLEYLFPRR
                     YPGKRYDYDRPLAVDSGVGACLLVRRQAMDEVGWFDERYFFFMEETDWAYRMRRQGWK
                     IYHVPSARIYHLQGQSIGPGVRSRIEFYRSRYQFFRKWHGLPYYALIRITIFLRLLVN
                     WFFTSLAVIFTLGLSRRIRSKLHVYSSLILWHAGLRDDGKA"
     gene            complement(1588..2427)
                     /locus_tag="PLT63_09385"
     CDS             complement(1588..2427)
                     /locus_tag="PLT63_09385"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOD98974.1"
                     /translation="MTPSEPSGRPGGAGGGAVNPGTGKAPLSVAVITQNEERNISACL
                     QSVAFAAQIVVVDSHSGDATAEIARASGAEVFAEPWRGGFGAQKQFAIDQCRQPWILI
                     LDADERIPPETAVVIARVLAEPGPAGYSFPRKNYFQGRWIRHAGWWPDRLTRLFRRGQ
                     GRMSVAAVHEGLEVEGPVAALDVPIVHCTESDLSLILRKIDHYSTLGAREAYAAGRRA
                     TVGGALVRAALTFLQDYVFRLGMLDGPQGLTLAATDGINKFCKYAKLAEMTRRRGRPA
                     GEP"
     gene            complement(2414..3115)
                     /locus_tag="PLT63_09390"
     CDS             complement(2414..3115)
                     /locus_tag="PLT63_09390"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOD98975.1"
                     /translation="MSIIKGYLGTKDTPAGALTKVLKAVTRRAARPTDICDHLATLFM
                     ECLGIEARLIVELGSGDGESTFVLERVAKLWDGALVSVDIEDREEVGTYPKRHFVKSD
                     DISFAALFPAWCAARGLAPAIDILFIDTSHRYEHTGEEIRHWFPYLSPRCKVVFHDTN
                     LQEVFVRRDGSRGQGWNNDRGVIRAIEDHFGRRFDESRDFFREDKGWLIRHWAHCNGL
                     LIMDRYEGGAAGDAF"
     gene            complement(3128..4129)
                     /locus_tag="PLT63_09395"
     CDS             complement(3128..4129)
                     /locus_tag="PLT63_09395"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOD98976.1"
                     /translation="MGRQAESGVIDGCRQERLRARRVERTLIPDITYLVLNYNPSGED
                     TACRVLEENLRAFYERKSPRLRGDVYLLDQGSPEGHRRRLLELQTRYGLSAILLNRNI
                     GISRAVNFLVRTAKSPVFALITSDVIVTSGMDEDLFQKVQIPEVYQATPFTDKSDVDH
                     QIWKPQAPYGTDHPDLTELQEKETTLKDKLFRRRRRGYLRYVGVEFNVMFWRREIFAA
                     IGYFDERWKAAYENNDFSLRCFLAGGCTALSVDSFVWHYHKVTEKNASREQAYPHCRQ
                     DWRGEMRRIWDEKWPELDRYFNIYKPLGEKTIRDFPAFHERFKGNIFLPYDQKIEYF"
     gene            4235..5530
                     /locus_tag="PLT63_09400"
     CDS             4235..5530
                     /locus_tag="PLT63_09400"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOD98977.1"
                     /translation="MPLKSLENNVAGATTPAPASEKKEKIYKYLHISLLVLIGVHVFI
                     NPFPQLTAIEEISFYGAAFVLAALLRSKQTTFSFQGPLTMPLFLFVGWTVVGLFFALD
                     KANTIHDIYAHLLKYLFFYFLLVNYFNTFRRFELLWLLLIFSTAVFAVYLMVYFYLII
                     GNPFAVKLGYKMPWEIPPNFIGVLTIFALLLSINIYNFKELKAYKLLLAIPAGILVIA
                     TLSTKTRGAIFSLFVAVLVLFARNKRNLLLFFLALVMLVVAMPVKERLSPEELTAKIQ
                     ADDRLQIWYTFWEMTKDHPITGIGFGMETYHDEDLLNKYNQRVPAAYRQSVPLKSPHN
                     FFVDLTVRTGFVGLAIFLFLLWRCFALAAALIKRGKSNFVRTWATGLTAALIAWMIQG
                     MFESIASGPAAKLFFVILAMLTILWQLREREEPVDAGLV"
BASE COUNT         1106 a         1729 c         1592 g         1181 t
ORIGIN      
        1 tcttttatac aggcccggca gtcggcgggc tagggcgctt tttacgatgc cgtcatttct
       61 gccaatgtat tcggttgtac atacagctcc gccctctgga ttcccggcgg gcggatatcc
      121 cctggcggcg gcccgggatc cgggccaggt cgaggccttg aaaaggctaa ggtcttaact
      181 ccgtgtccga ccggccctgg acatgggtct ccacgccgga caactcattg cgcagttcga
      241 ctatctctct ttccagacgc tcgtactccg ccagcttgcg cttcaggaag taaaaggtcg
      301 tgcgggcctt ttcctcgatc ccgctgggcg tcagtttgta gagataggaa atcttgttgc
      361 tggagctctt gaagttgtct accttgacca ggccgcgccg gatcagggac ttgatcagat
      421 agttgacctt gccgagactt atgcccaggc gcgaggacag ctcccgctgg gtcagggccg
      481 gttcttcctt gatcgccttg agtatcttca ggacgtcttc attgttcaac ggcggcattt
      541 ccggtttcga caagcggatt ctccttgcgt tcaatgcatg aacatgcgcc gttcctaaac
      601 cagcgcggat agaaagtcaa gggaaattta ttgaaagcta cgctttgccg tcgtcccgaa
      661 ggcccgcatg ccaaaggatg agacttgaat agacatgcag tttgctgcgg atgcgccggg
      721 aaagcccgag ggtgaaaata accgccaggg aggtaaaaaa ccagttgacc agcaggcgga
      781 ggaagatggt gatccggatc agcgcatagt agggaaggcc gtgccatttg cggaaaaact
      841 gatagcggga ccgatagaat tcgatccggg accggacgcc ggggccgatg ctctgaccct
      901 gaagatgata gatccgggcg gacggcacat gatagatttt ccagccttgc cggcgcatcc
      961 ggtaagccca gtccgtctct tccatgaaga agaaataccg ctcgtcgaac cagcccacct
     1021 catccatggc ctgtcttctc accagcaggc aggcgcctac cccggaatcg accgcgaggg
     1081 gccgatcgta atcgtaacgc ttgccggggt agcggcgggg gaaaagatat tccagaaccg
     1141 gcatgttgaa cagcagggta aacagcgtcg gaaaagcggc gatggaattc tgcttcgtcc
     1201 cgtcccggtt gagaagctga ccgcaggcca tggcggcgtc cgggcgggat tccataaagg
     1261 aaaacagctc ccggacggcg tcttccgtca ccaccgcgtc ggtgttgagg agcagggcat
     1321 agcgcccccg catcacggca aaggcctgat tgttcgccgc gccgaacccg cggttcgttt
     1381 cattgacgat cacccgcgcc gcggggaaac actcccgcag catggcgaca ctgccgtcgg
     1441 tggaggcgtt gtccaccaca atgatctcac aggttgggcc gccgcgggtc ccccggagcg
     1501 acgccaggca gtttttcagc agttccccgg tgttccagtt gacgacgatc accgagacat
     1561 ccggcgtttt cccccccgcc ggcatgctca tggctccccc gccggccgtc cccggcgccg
     1621 ggtcatctcc gccagcttgg cgtatttgca gaacttattt atcccatcgg tggcggccag
     1681 cgtgaggccc tgcgggccgt cgagcatccc cagccggaat acgtaatcct ggagaaaggt
     1741 caatgccgcc cggaccaggg cgccgcccac cgtggcccgc cggccggcgg cataggcctc
     1801 ccgggcgccc agagtcgaat agtggtcgat cttccggaga atcaggctca gatcgctctc
     1861 cgtgcaatgg acaatgggga cgtcgagggc cgccacgggg ccctcgacct ccaggccttc
     1921 gtgaacagcg gcgacggaca tccggccctg cccccggcgg aaaaggcggg tgaggcggtc
     1981 gggccaccaa ccggcatgac ggatccaccg cccctgaaag tagtttttcc ggggaaagct
     2041 gtaaccggcg ggaccgggct cggcaaggac cctggcgatc accacggccg tttccggagg
     2101 gatgcgctcg tcggcgtcca ggatcaggat ccacggctgg cggcactgat cgatggcgaa
     2161 ctgcttctgg gcgccgaatc cgccccgcca gggctccgca aagacttcgg cgccggacgc
     2221 ccgggcgatt tccgcggtgg cgtcgccgct gtgggaatcc acgacgacga tctgggcggc
     2281 aaacgccacg ctttgcaggc aggcggagat attacgctct tcattttgcg tgattacggc
     2341 cacggagagg ggagccttac cggtgccggg attcaccgcc ccgccccccg ctcccccggg
     2401 ccggccggaa ggttcagaag gcgtcaccgg cggcgcctcc ctcatagcgg tccatgatca
     2461 acagaccgtt gcaatgggcc caatggcgaa tcagccaccc cttgtcctcc cgaaagaaat
     2521 cccgggattc atcgaaccgc cgcccgaaat ggtcctcgat cgccctgatc accccccggt
     2581 cgttgttcca gccctggccc cggctgccgt cccggcggac gaacacctcc tgaaggttgg
     2641 tgtcatggaa gacaaccttg caccgggggg acaggtaagg gaaccagtgt cttatctcct
     2701 cgccggtgtg ctcgtagcga tggctggtgt cgatgaacag gatgtcgatg gccggcgcga
     2761 gcccccgggc ggcgcaccag gcggggaaaa gggccgcgaa ggagatgtcg tcgcttttca
     2821 cgaaatgccg cttcggatag gtgccgactt cttcccgatc ttcgatatcg acgctcacca
     2881 gggcgccgtc ccagagtttc gccacccgtt ccaggacaaa ggtggactcg ccgtcccccg
     2941 atcccagctc cacgatcagt cgcgcctcga tccccagaca ttccataaac agggttgcga
     3001 ggtggtcgca aatatccgtc ggccgcgccg cccgccgcgt cacggccttc aggaccttgg
     3061 tgagggcccc cgccggggtg tccttggtgc cgagatagcc ctttatgatc gacattatcg
     3121 ataatcctca aaaatattcg attttttggt cgtaaggcag gaagatgttg cccttgaacc
     3181 gttcatggaa ggcgggaaag tcccggatcg tcttttcccc caggggtttg tagatattga
     3241 aataacgatc cagctccggc catttctcgt cccagatccg ccgcatctcc ccgcgccagt
     3301 cctgccggca gtgcggatag gcctgctcgc gggaggcgtt tttctccgta accttgtgat
     3361 agtgccagac gaaggaatcg acgctcaggg cggtacaccc cccggcgagg aaacagcgga
     3421 gggagaaatc gttattctca taggcggcct tccagcgctc atcgaaataa ccgatcgcgg
     3481 cgaatatttc ccgcctccag aacatgacgt tgaattccac tccgacataa cggagatagc
     3541 cgcgccgccg ccgccggaaa agtttatcct tcagcgtggt ttccttttct tgaagttccg
     3601 tcagatcggg atggtccgtc ccgtagggcg cctgcggttt ccagatctgg tggtccacat
     3661 cgcttttgtc ggtgaacggg gtggcctggt aaacttccgg gatctggacc ttctggaaca
     3721 gatcttcgtc cattcccgag gttacgatca cgtccgaggt gatcaacgca aagaccgggg
     3781 actttgccgt gcgcaccaga aaattgaccg cccggctgat gccgatgttg cggttcagga
     3841 ggatcgccga aaggccgtat ctggtttgca gctccaggag gcgccgccga tgcccttccg
     3901 gggaaccctg gtcgagaaga tacacgtccc cccgcaggcg ggggctttta cgttcataaa
     3961 aggcccggag gttctcctcc agcacccggc aggccgtatc ttccccggag ggattatagt
     4021 tgagcaccag ataagtgatg tccggtatca atgtccgttc tacccgcctt gcccgaagtc
     4081 tttcctgccg gcaaccgtcg atgacgccgc tctccgcttg tcgtcccata ttcatccgcc
     4141 tataacacaa acggtcatgc aattaaatga tgaattcccc caatgactat gctacaaaag
     4201 gcgtaatttg agcttccgta aggattgttt ttttatgcca ttgaaatcac tggaaaataa
     4261 cgtcgctgga gcaacgaccc cggccccggc gtctgaaaaa aaggaaaaaa tttataaata
     4321 tctccacata tcgctcctgg tcctcatcgg ggtgcatgtc ttcatcaatc cctttccgca
     4381 actcaccgcc attgaagaaa taagttttta cggcgccgct tttgttctgg cggcgctgct
     4441 caggtcgaaa cagacgacct tcagcttcca gggtccgctg accatgccct tgtttctctt
     4501 tgtcggctgg acggtggtcg gcctgttctt cgccctcgac aaggccaaca cgatccatga
     4561 catctacgcc catctactga aatacctgtt tttttatttt ttattggtga actatttcaa
     4621 tacctttcgt cgttttgagc tcctatggct gctgctcatt ttttccaccg ccgtctttgc
     4681 ggtctatctc atggtgtact tttacctaat catcggcaat ccgttcgccg tcaagctggg
     4741 atataaaatg ccctgggaga tcccccctaa cttcatcggc gttctgacga tattcgccct
     4801 gcttttatcg atcaacatat acaatttcaa ggaattgaaa gcatacaagc tgctgctcgc
     4861 catcccggcg ggcatcctgg tcattgccac cctgtccacc aagacgagag gggccatctt
     4921 ttccctcttc gttgccgtcc tggtcctgtt cgcccgcaac aaaaggaacc tgctgctgtt
     4981 ctttctggcc ctggtcatgc tcgtcgtcgc gatgcccgtc aaggaacgcc tttcccccga
     5041 agagttgacc gccaagatac aggccgacga ccggctgcaa atctggtaca ccttctggga
     5101 gatgaccaag gatcacccca taaccgggat cggattcggg atggaaacgt atcacgacga
     5161 ggacctgctg aacaaataca accagcgcgt ccccgccgcc taccgccaga gcgtccccct
     5221 taaatcaccc cacaacttct ttgtggatct caccgtccgg acgggattcg tcggcctggc
     5281 cattttctta ttcctcctct ggcgatgctt cgccctggcc gccgcgctca tcaaacgggg
     5341 gaaaagcaat ttcgtccgca cctgggccac ggggctgacc gccgccctga tagcctggat
     5401 gatccagggg atgttcgaga gcatcgccag cggacccgcc gcgaagttgt tttttgtcat
     5461 tctggccatg ttgaccattc tctggcagct ccgggaacgg gaagaacctg tggacgcggg
     5521 gctcgtctga agatgcaaaa gcgccgccgc gtagccgtcg tcatccccaa gtacggcctg
     5581 gtgggcgggg ccgagggctt cgccgccg
//
LOCUS       DAOSMF010000122         5563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_15982_length_5563_cov_21.910312, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000122 DAOSMF010000000
VERSION     DAOSMF010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5563
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15982_length_5563_cov_21.910312"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="PLT63_09405"
     CDS             complement(<1..224)
                     /locus_tag="PLT63_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOD98978.1"
                     /translation="MNKGDLVNAVVSVVGKKKTAEEAVNCVLETITGALKKDEAVTLV
                     GFGTFKVFKRAARTGRNPQTGKAIKIKAKKV"
     gene            complement(601..1128)
                     /locus_tag="PLT63_09410"
     CDS             complement(601..1128)
                     /locus_tag="PLT63_09410"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HOD98979.1"
                     /translation="MRKATSTIFAVLVILALATTSFAYGWGQGPRSGRGPGYGPCQSG
                     EVRGLAGVNLTAEQESKIKSLRDAHLKEIKPLQDQLFSKRGELKLLWLQQQPSEEKIT
                     AVQKEIRNLRDRLQDKQTSHRLAVLKVLTPEQQTKLQTYGAMRGFGPGKGFARGGGYG
                     PGGCGGYGPGPQGNR"
     gene            1389..3146
                     /locus_tag="PLT63_09415"
     CDS             1389..3146
                     /locus_tag="PLT63_09415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD98980.1"
                     /translation="MEKAPSLKQRWSWTIISPWLIIGAALVLVPLFVFMTMQSLNRQK
                     TETNRLLLEKGDALIRSFEAGARTGAGLRWDAFRLQKLLIETAQQPGIDYFIITDATG
                     VILADSDPYRIGDFYGANLDLARLVAVKTAQWRQVANEEGADTFEVYRGFTPTPGPFP
                     IWQERHADPRTPPGLVIFVGLDMGPVLAARKADEQHTIWMGVVLIMVGLSGVISLLLA
                     QGYKATRRSLFQVRAFSAHLMENMPMGLIAIDPQGVIMAYNKTAAVILAVPEAEARQR
                     PAAAFLPPACREIIASATTAREPIVREVTCRFRDGRTAPLEIIASRLQEPEGELMGLV
                     ILFRDISDLRTLQEEIARTKHLAALGSLAAGVAHEIRNPLSSIKGFATYFRERYSDVP
                     DDRETAEIMIGEVERLNRVIGQLLEFARPTTLQSTPDQIAAVIEACLKIVTGDLATKG
                     ITVEKELPPLPDVSYDRDRMKQVFLNLFLNALAAMEPGGVLTVTAREEGERWIRIEIA
                     DTGAGIAAADLERIFDPYFTTKPAGTGLGLAIVQKIMAAHHGEIKLESAPGKGTRAII
                     RLPIKRSAPDGVPLPKEKK"
     gene            3308..4804
                     /locus_tag="PLT63_09420"
     CDS             3308..4804
                     /locus_tag="PLT63_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOD98981.1"
                     /translation="MTEKYTILVVDDDRAHRTMLKTLLSGWGYLIREADDGETAIAAV
                     RERAFDLVLMDIRMLKISGLEALVEIKAINPAIPVIIMTAYASLETAVEAVKKGAYDY
                     LQKPLDFDELRLKMERAMEHRRLKEENALLKESLGARFDRRNLIGSAPVMVRLLETVA
                     QVAPSEATVLITGESGTGKEMLAGAIHFNSQRREGPFVKINCAAITETLLESELFGHE
                     KGAFTGADRRKEGKFRQADGGTIFLDEVSEMSLAMQVKLLRVLQEREITRVGGSEAVK
                     VDVRVIAATNRDLAAGIAAGRFREDLFYRLNVVTLTAPPLRERREDIPILAQHFLTHF
                     AEKNHKQIKGFTPQALDRLRRHSWPGNVRELMNAVERGVVLSRGDYLEEAELSLSPAP
                     GAVPEPPAPAGMPELFAPAATGSAFTATGTAATTTAIGIPGAALPSAPGAETTAGEKR
                     PVSLNEVEQETIIRTLAEVKGNKSEAARRLGVTRRTLHLKLKKYGLME"
     gene            4822..>5563
                     /locus_tag="PLT63_09425"
     CDS             4822..>5563
                     /locus_tag="PLT63_09425"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98982.1"
                     /translation="MNILVCIKQILAPEAVSEGPGSLGSNVAGNEAPAAKGGARIFFE
                     PSFADDCYRMNPYDEHALEEALRIRERFPEAKIDALTVGPDRCRQVLRRALEMGADRA
                     VLLQVASAPRKELVSPHSLEPATAAGGAGLLFSKELVSPSSEDPGRSSWGEPGWPASE
                     ALVSPSSGEPVPPHARSDSKPAHPGDTSPPQDLTPYETAAIIAAYLRDKTHDLILAGV
                     MAEDGMAGQTGQLLAELLGLPCATAVISE"
BASE COUNT         1109 a         1779 c         1618 g         1057 t
ORIGIN      
        1 acctttttgg ccttgatctt gatggccttg ccggtctggg gatttcttcc ggtgcgggct
       61 gctcttttaa agaccttgaa ggttccgaag ccgaccaagg taacggcttc atccttcttc
      121 agggccccgg ttatggtttc cagaacacag ttgaccgctt cctccgccgt tttcttcttc
      181 cctaccactg aaaccaccgc atttaccaga tctcccttgt tcatctcttg cctccttttt
      241 tatttatcgt ccaccgccct gcacggttca acgacctgac aattagttat cactttttgc
      301 ggccgattat aaaagagcga aagtcttgtg tcaagggtaa tttcacgttt tttcagcttt
      361 atttatgcgt attgcatttg atatttattg tataataatg agaatttaat ttgatatttt
      421 taacaagata ctggggagag tcattatcat tatttaaata gcgggaggcc cacggcgaca
      481 acgacatgaa cggcgaccta aaaagaagcc ctcccccgga aaggggaggg cttgggaagg
      541 ggttgggggg gcgggatctt tctctatccg tcgccccggg gccgatggcg gcgtttcggg
      601 ttaccggttg ccctggggac cagggccata accgccgcaa ccgccgggac cgtagccgcc
      661 accgcgggcg aatcctttac cggggccgaa gcctctcatg gcgccgtagg tctgcagctt
      721 ggtctgctgt tccggggtca ggaccttcag gacggccagg cggtgactcg tctgcttgtc
      781 ctgaagtcga tcccgcaggt tacggatctc tttctgcacc gccgtgatct tctcctcgct
      841 gggctgctgc tggagccaca ggagctttaa ctctccccgt ttgctgaaaa gctgatcctg
      901 aagcggcttg atctccttta aatgggcgtc ccgcagggac ttgatcttgc tttcctgttc
      961 cgccgtcagg ttaactccgg ccagacctct tacctcgccg ctctgacagg gaccataccc
     1021 gggtcccctg ccgctgcggg gtccctgtcc ccagccgtag gcgaaggaag tcgtcgccag
     1081 ggccagaatc acgaggaccg caaaaattgt cgatgttgcc tttctcattt tttttctctc
     1141 ctttccgatc tctttatttt ctattttcgt tgcttacctt atcttcttct ctttgccctc
     1201 ttggattgca actgccgtgc caccatgcca catacattac atgtaactaa ttatacttat
     1261 ttatttgccg atcgacccag aagtgttcgc ctcgtcgccg cggtaaagaa aaaactggct
     1321 aataattatc cgcctatgct atccaaacgt ctaaaaagta tccgctcaac aaccgcggca
     1381 ggcaaggaat ggagaaggca ccctcgctaa aacaacgctg gtcctggacc atcatctccc
     1441 catggctcat catcggggca gcgctggtgc tggtgcccct gttcgtcttc atgaccatgc
     1501 aaagcctgaa ccggcagaaa acggaaacca accgcctgct gctggaaaag ggcgacgccc
     1561 tgatccgctc cttcgaggcc ggggcacgca ccggcgccgg cctgcgctgg gacgccttcc
     1621 ggctccagaa actcctcata gaaacggcac agcaaccggg catcgattac ttcatcatca
     1681 ccgacgccac cggggtaatc ctggccgaca gcgacccgta ccggatcggc gacttctacg
     1741 gcgccaacct cgatctggcc cgtctcgtcg ccgtcaagac cgcccaatgg cgccaggtgg
     1801 ccaacgaaga gggcgccgat accttcgagg tgtaccgggg tttcacccct acccccggtc
     1861 ccttccccat ctggcaggaa aggcatgccg atccccggac gccgccgggg ttggtgatct
     1921 tcgtcggcct ggatatgggt ccggtcctcg ccgctcggaa ggccgatgaa cagcatacca
     1981 tctggatggg ggtggtgctc atcatggtgg gactttccgg ggtcatctcc ctgcttctgg
     2041 cccagggcta caaagcgacc cggagatccc tgttccaggt ccgggccttc tcggcccatc
     2101 tcatggaaaa catgcccatg gggcttattg ccattgatcc ccagggggtg atcatggcct
     2161 acaacaagac cgccgccgtc atcctggccg ttcccgaagc ggaggcacgg cagcggcccg
     2221 cagcggcgtt cctcccgccg gcctgccggg aaatcatcgc cagcgcgacg acggcacggg
     2281 aaccgatcgt ccgggaggtg acgtgccgat tccgggacgg ccggacggcg ccgctggaga
     2341 tcattgcctc ccgtctccag gagcccgaag gcgaattgat ggggctggtc atcctcttta
     2401 gggacatctc cgacctccgg acgctacagg aggagatcgc ccggaccaaa cacctggccg
     2461 ccctgggcag tctcgccgcc ggggttgccc acgagatccg caaccccctg agttccatca
     2521 agggtttcgc cacctacttc cgggaaagat acagcgacgt tccagacgac agagagacgg
     2581 cggagatcat gatcggcgag gtggaacgcc tcaaccgggt gatcggccaa ctgctggaat
     2641 tcgcccgccc cacgactctt cagtccaccc cggaccagat cgcggcggtc atcgaagcat
     2701 gcctgaagat cgtcaccggc gacctggcaa ccaaagggat caccgtggaa aaggaactgc
     2761 ctcccctgcc cgacgtttcc tatgaccggg acaggatgaa gcaggtcttc ctcaacctct
     2821 tcctgaacgc cctggcggcc atggaaccgg gcggcgtcct caccgtcaca gcccgggagg
     2881 aaggggaacg atggatacgg atcgagatcg ccgacaccgg cgccggcatc gccgcggcgg
     2941 atctggagcg gatcttcgac ccctatttca ccaccaaacc cgcgggaacg ggtctgggcc
     3001 tggccatcgt ccagaagatc atggcggcgc accacgggga gataaagctg gaaagcgctc
     3061 caggaaaggg aaccagggcg atcatccgcc tgcccattaa acgtagcgcc cctgacggcg
     3121 tgccgctacc aaaggaaaag aaatgagaat acgaaaccca tccccacctt aatccttgaa
     3181 ggagaggaaa ctgcccgtat ttgcaagatg aaaatacatc cgcgcgatct cccccaaaca
     3241 tttttcatca tgcgtgggtg ttggataaac atgacaattc atttgatatt ttaaggaggg
     3301 attgttggtg acggaaaaat acactatcct cgtggtggac gacgaccggg cccatcgcac
     3361 catgctgaag accctgcttt ccgggtgggg atacctgatc cgggaggccg acgacgggga
     3421 gacggccatt gccgccgtcc gggagcgagc cttcgacctg gtcctcatgg acatccggat
     3481 gctcaagatc tccgggctgg aggccctggt ggagatcaag gccatcaatc ccgccatccc
     3541 ggtcatcatc atgaccgcct acgcctccct ggaaaccgcc gtggaggccg tcaagaaagg
     3601 ggcctacgac tatctgcaga agcccctgga cttcgacgaa ctgcgtctca agatggaacg
     3661 ggccatggaa caccgccgcc tcaaggagga aaacgccctg ctgaaagaga gcctgggtgc
     3721 ccgcttcgac cgtcggaacc tcatcggcag cgccccggtc atggtgcggc tcctggaaac
     3781 cgtggcccaa gtcgccccct cggaggccac ggtcctcatc accggcgaat cggggaccgg
     3841 caaggagatg ctcgccgggg ccatccattt caacagccag cgccgggagg ggcccttcgt
     3901 gaagatcaac tgcgccgcca tcacggaaac ccttctggaa tcggagcttt tcggccatga
     3961 gaaaggggcc ttcaccgggg cggaccgccg caaggagggg aagttccgtc aggccgacgg
     4021 gggcacgatc tttctcgacg aggtgagcga gatgtccctg gccatgcagg tcaagctcct
     4081 ccgggtcctc caggagcggg agatcacccg ggtggggggt agcgaggccg tcaaggtgga
     4141 tgtccgcgtc atcgccgcca ccaaccgcga cctggccgcg ggtatcgccg ccggtcgttt
     4201 ccgggaggac ctcttctacc gcctcaacgt cgtgacgctc acggcgccgc ccctgcggga
     4261 gcgccgcgag gacatcccca tcctggccca gcactttctt acccacttcg ccgagaagaa
     4321 ccataagcag atcaaggggt tcacccccca agccctggac cgtctccgcc gccactcctg
     4381 gccgggcaac gtccgagaac tgatgaacgc cgtggaacgg ggggtggtcc tgtcccgggg
     4441 ggactatctg gaggaggcgg aactgtcgtt gagcccggcg ccgggcgccg ttccggaacc
     4501 gcccgcaccg gccggcatgc cggaactgtt cgcgccagcc gccaccggca gtgccttcac
     4561 tgccacgggc accgcggcaa ccaccaccgc catcggcatt cccggcgccg cacttccgtc
     4621 tgcccccggc gccgaaacga cggccgggga aaaacgtccc gtctccctca acgaggtgga
     4681 acaggagacg atcatccgca ccctcgccga ggtgaaaggc aacaagagcg aagcggcccg
     4741 gcgcctcggc gtcacccgcc gcaccctcca cctgaaactg aaaaaatatg gattaatgga
     4801 atgaaacata ggaaatacag catgaacatt ctggtctgca ttaaacagat cctggcgccg
     4861 gaggcggttt cggaaggtcc gggcagtctg ggcagcaacg tagcgggaaa tgaagccccg
     4921 gccgccaaag ggggggcgcg gattttcttc gagccgtcgt ttgccgacga ctgctaccgg
     4981 atgaacccct acgacgagca cgccctcgag gaggccctgc ggatccggga acggtttccc
     5041 gaggcgaaga tcgacgccct caccgtggga ccggaccgct gccgccaggt cctgcgccgg
     5101 gcgctggaga tgggggcgga ccgggccgtc ctgctccagg tggcgtcagc gcccaggaaa
     5161 gagctggtat cgccgcattc tttagagccg gcgacggcgg ccggcggggc cggactgctg
     5221 ttttcgaaag agctggtgtc accgtcctca gaagatccgg gacggtcgtc ctggggagag
     5281 ccgggatggc cggcttcgga agcgctggtg tcaccgtctt cgggggagcc ggtgccgccg
     5341 catgctcgat cggactccaa gcctgcccac cccggtgata cctccccccc ccaagacctg
     5401 acaccctatg aaaccgccgc cattatcgcc gcctatctgc gggataaaac ccatgacctg
     5461 atccttgccg gcgtcatggc cgaagacggc atggccggcc agacggggca actgcttgcg
     5521 gagctcctgg ggctaccctg cgccaccgcc gtcatcagtg aac
//
LOCUS       DAOSMF010000123         5513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_16174_length_5513_cov_20.738182, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000123 DAOSMF010000000
VERSION     DAOSMF010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5513
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16174_length_5513_cov_20.738182"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..950
                     /locus_tag="PLT63_09430"
     CDS             <1..950
                     /locus_tag="PLT63_09430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HOD98983.1"
                     /translation="KRLRKEERRMRIGIVGLGGVGGAFGGRLAMRYGEAPDHEVVFIA
                     RGEHLKVIHDQGLRLVTPDGELIARPSLATDDPSAAGPLDLVLFAVKSYGLEGAARSI
                     AGNIHDRSVVIPLLNGVDITELLQGILPRGVFLYGTVYVSAYIEAPGVVRQVSPASRL
                     IFGPASGGVEGFHGIDRLFQDAGIVSELTSDPIAALWTKFIFICPAASVTSLTRKSFG
                     AVMDDPEARGMLTGMITEVEQIARAKGVELPGDIAAATLAKVRQFPPETKTSLQLDFE
                     KGRPTEIDTFTGFIVRAGRKLGIATPLHDRVYETLADKA"
     gene            complement(1124..2005)
                     /locus_tag="PLT63_09435"
     CDS             complement(1124..2005)
                     /locus_tag="PLT63_09435"
                     /inference="COORDINATES: protein motif:HMM:NF008818.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     beta"
                     /protein_id="HOD98984.1"
                     /translation="MQLEAMDRELMCSGHVGCPGCGATIAMRFMLKALGEKTVMVLPA
                     CCWSVIAGPFPQSSLKIPVIHTAFETGGATASGVRAALDMRGDEETTVVTWAGDGGTF
                     DIGFQALSGAVERNENFIYICYDNEAYMNTGIQRSSSTPYGAWTTTTSGSDWKKLRKK
                     NIVEALVAHRIPYAATANIAYPEDLVRKVKKAKEVKGSRFLHIYASCPTGWRIPSEMS
                     IKIARMAVQTNIFPLYEVENGVNYTINVKPREYLVREYFKLQGRFRHLSEEDLDQIQA
                     MVNEDWELLLRKAGEHA"
     gene            complement(1995..3164)
                     /gene="porA"
                     /locus_tag="PLT63_09440"
     CDS             complement(1995..3164)
                     /gene="porA"
                     /locus_tag="PLT63_09440"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HOD98985.1"
                     /translation="MKKVLEGSQAVAEAVRLAKVQVISAYPITPQTHIVEILSDYVTS
                     GRMNAKYLRVESEHSCMAALIGAQSTGVRTFTATCSQGLALMHELLHWASGARLPIVM
                     GEVNRALAPGWNIWSDQTDSLAQRDTGWMQLYCEDSQEVLDTTILAYRLAERVNLPAM
                     VVLDAFFLSHTYEPVDVPEQEEVDRFLPPYNPVFILDPKHPYSLSPLVTPNAYLEMRH
                     DIALAMEDALAIFDEVEGEFARILGRRYGVVEEVNCDDADIIFVTSGTVTSTARLALE
                     ELRSRGEKVGALKIKLFRPFPVEAVRRALSKAWKVIVVDRNFSFGASGIFAQEVRAAL
                     CNAPGHPPVFGYIAGLGGRDITMQMLSDIYFEVKRSEAPRRESVWVGLQEVKRAT"
     gene            complement(3161..4921)
                     /locus_tag="PLT63_09445"
     CDS             complement(3161..4921)
                     /locus_tag="PLT63_09445"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOD98986.1"
                     /translation="METPTALQNILYMPRTSTTTESNKTGSWRFLRPRYEEKTSPCSA
                     ACPAGEDIARIEMLITQGAFKEAWETILMENPLPGVCGRVCYHPCEGRCNRREFDAAV
                     AIHTLERFLADAAARNETKPALPRQPATGRQIAIIGSGPAGLSAAWFFNILGYETEIF
                     EAMPAAGGMLRWGIPAYRLPEAVLNAEIARIEAQGVRIRTGEAVSAARFEEMRKSYDA
                     LFLGCGYWRDRPLDIPGAAGPGVEDGLSFLREIRGGVKRDVPGLSAVIGGGNTAIDVA
                     RSIIRRGGRAIIVYRRRREDMPAFTEEVQSAREEGVELRELLAPVRIDAGGGDCVLTL
                     QPMAIAGQDRDGRGKIVPAAEAPVTITVQRIFTATGAGAAAPWHNPPVPGAPGAICSL
                     SHCVLLQDDRGAPVVYGGDLATGDKNVTQAIASGKQAALALDVFFRHGAGSMRERLAS
                     CLVGGGPSLSLEIYLGGPRRERNAHIVTYEEINADYFQYEPRVVQPRLLIEERHSSFD
                     EIELKISAHTAMREAGRCFNCGICNGCDNCYLFCPDVSILRSRDMSDRHIDYEYCKGC
                     GLCVVECPRNAMTLDEEGTP"
     gene            complement(4931..>5513)
                     /locus_tag="PLT63_09450"
     CDS             complement(4931..>5513)
                     /locus_tag="PLT63_09450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOD98987.1"
                     /translation="RMGGEKMLEIRFHGRGGQGTVVATILLSKAFFQAGWEVQSFPFF
                     GVERRGAPVEAYLRLDKKKILVRTNVTAPDHVVVQDQTLLQNIDVTRGMKPGGWVLLN
                     SPALPENLDPYRGMKLACVDATRIALRHLLGSRTHPIVNTAMIGAFARMLAMPPLEAA
                     CGAIREEAPSDPEANIAAARDAYNEVILHGSIS"
BASE COUNT          954 a         1703 c         1831 g         1025 t
ORIGIN      
        1 aaaaacgcct caggaaggag gaaagacgaa tgaggattgg aatagtcggt ttggggggag
       61 tcggtggggc cttcggcggt aggttggcga tgcgttatgg agaagcgccg gatcatgagg
      121 tggtgtttat cgcccgcggg gagcacctga aggtgatcca tgaccagggg ctccgtctgg
      181 taacgccgga cggggaattg atcgcccggc cctccctggc gacggacgac ccgtcggcgg
      241 cagggccctt agacctggtc ctgttcgccg tgaaaagcta cggtttggaa ggggcggccc
      301 ggtccattgc cggtaatatc catgaccgca gcgtcgtcat tccgcttctc aacggtgtcg
      361 atatcacgga gctgcttcag ggtatcctcc cccggggagt tttcctctac ggtacggttt
      421 acgtttcggc ctatattgag gcccccggcg tagtccggca ggtgagcccc gccagccggt
      481 tgattttcgg gcccgcctcc ggcggagtgg agggtttcca tggtatcgac cgtttgtttc
      541 aggacgccgg catcgtctcg gagctgacct ccgatcccat agcggcgctg tggacgaaat
      601 tcatcttcat ctgccccgcc gccagcgtga cgtccctgac gcggaagtcc ttcggcgccg
      661 tgatggacga tcccgaggcg aggggaatgc tcaccggcat gatcaccgag gtggagcaaa
      721 ttgcccgggc caaaggggtg gaactgcccg gcgatatcgc cgcggcgacc ctggccaagg
      781 tccggcagtt tcccccagag acgaagacct ccctccagtt ggatttcgag aaggggcggc
      841 cgacggagat cgacaccttt accggcttca tcgtcagggc cggccggaag ctggggatcg
      901 ccacccccct tcatgaccgt gtgtatgaga ccctggcgga caaagcttga accgagttga
      961 atcctgcccc aaggcgcgtg aaatcaagga agaaagatac aaaggggcca ttccgggcgc
     1021 ccccccccgc gagatcgggg cgaaggaatg gcccatttca ttccgggtcg cccgagtgcg
     1081 ggaaagggaa ggaacgcggc agtgcggaag tccctttcat ccctcaggca tgttcccccg
     1141 ccttgcggag gaggagttcc cagtcttcgt tgaccatggc ctggatctgg tcgaggtcct
     1201 cctcgctcag atggcgaaag cgtccctgga gcttgaagta ctcccggacg agatactccc
     1261 ggggtttgac gttgatcgtg tagttgaccc cgttctctac ctcgtagagg gggaagatat
     1321 tggtctgaac ggccatgcgg gcgatcttga tggacatctc cgaggggatc cgccaccccg
     1381 tcggacagct cgcgtagatg tgcaggaacc gcgatccctt cacctccttg gcctttttca
     1441 ccttacggac cagatcttcc gggtaggcga tgttggccgt ggctgcgtag gggatgcggt
     1501 gggccaccag ggcctcgaca atgttcttct tccgcagctt cttccagtcc gagccgctgg
     1561 tcgtggtggt ccaggccccg tagggggtgg aggaactccg ctgaatcccc gtgttcatgt
     1621 aggcctcgtt gtcgtagcag atgtagatga aattctcgtt tctttccacg gcgccgctca
     1681 aggcctgaaa gccgatgtcg aaggtcccgc cgtccccggc ccaggtgacc accgtcgtct
     1741 cctcgtcccc ccgcatgtcc agggccgccc ggactccgct ggcggtggcg cccccggtct
     1801 cgaaggcggt gtggatcacc gggatcttca gggaggactg agggaagggg ccggcgatga
     1861 cggaccagca gcacgccggc aggaccatga cggtcttttc ccccagggcc ttcagcatga
     1921 aacgcatggc gatcgtggcg ccgcatcccg gacagcccac gtggccggag cacatcaatt
     1981 ccctgtccat ggcttcaagt tgcacgcttc acctcctgga gccccaccca gacgctttcc
     2041 cgccggggcg cttcactcct tttcacctcg aaatatatat cggacaacat ttgcatggta
     2101 atatcccgcc cgcccagacc ggcgatgtag ccgaagaccg gcggatggcc gggagcgttg
     2161 cacagggcgg cccggacctc ctgggcgaag atcccgctgg ccccgaagga aaaattgcga
     2221 tccaccacga tgaccttcca ggccttgctc agggcacggc gcacggcttc caccgggaag
     2281 ggtcggaaga gcttgatctt cagcgccccg accttttcgc cccgggaccg gagttcctcc
     2341 agggccagcc gggccgtgct ggtaaccgtg ccggaggtga cgaagatgat gtcggcgtcg
     2401 tcacagttga cttcttccac cacgccgtaa cggcgaccca gaatccgggc gaattcacct
     2461 tccacctcgt cgaatatggc cagggcgtct tccatggcca gggcgatgtc gtgacgcatc
     2521 tccagataag cgttgggcgt caccaggggg ctcagggagt agggatgctt cgggtcgagg
     2581 atgaagaccg gattataggg gggaaggaag cggtccacct cctcctgttc cgggacatcc
     2641 accggttcgt aggtatggga gaggaaaaag gcgtccagca ccaccatggc gggcaggttg
     2701 accctttccg ccagccggta ggccagaatc gtggtgtcca ggacctcctg ggaatcctcg
     2761 cagtagagct gcatccaccc cgtgtcccgt tgggcgaggc tgtcggtctg atcgctccag
     2821 atgttccagc ccggagcaag ggcgcggttg acctcgccca tgacgatggg gaggcgggcc
     2881 ccggaggccc agtggaggag ctcgtgcatc agggccagcc cctgggagca ggtggccgtg
     2941 aaggttcgca cgccggtgct ctgggcgccg atcaaggccg ccatgcagga gtgctcgctt
     3001 tccacccgga gatatttggc gttcatccgg ccggaggtga cgtaatccga taggatctcc
     3061 acgatatggg tctggggggt gatgggatag gcggaaataa cctgcacctt ggccaaacgc
     3121 accgcctccg ccacggcttg gctgccttcg aggacctttt tcatggtgtt ccctcctcat
     3181 ccagggtcat ggcgttccgg ggacactcca cgacgcacag accgcaccct ttgcagtact
     3241 cgtaatcgat gtgccggtcc gacatgtccc gggaccgcag gatggagacg tcgggacaga
     3301 acagataaca gttgtcgcag ccgttgcaga tgccgcaatt gaagcaccgg ccggcctccc
     3361 gcatggccgt atgggccgat atcttcaact cgatttcgtc gaaggatgaa tggcgctctt
     3421 cgatgaggag ccggggctgg acgacccggg gttcatactg gaaatagtcg gcgttgatct
     3481 cttcataggt tacgatatgg gcgttccgtt cccggcgcgg accgccgaga tatatctcca
     3541 gggagaggga aggcccgccg ccgaccagac aggaagcaag gcgctccctc atggacccgg
     3601 caccgtggcg aaaaaagaca tccagggcca gggcggcctg cttgcccgag gcgatggcct
     3661 gggtgacatt cttgtcgccg gtggccaggt caccgccgta aaccaccggc gcgccccggt
     3721 cgtcctgaag gaggacgcag tggctgagac tgcagatcgc tcccggcgcc cccgggacgg
     3781 gggggttatg ccagggggcg gcggccccgg caccggtggc cgtgaagatc cgctgcacgg
     3841 taatcgttac cggcgcctcg gcggcgggga cgattttccc ccgtccgtct cggtcttgtc
     3901 ccgcgatggc catgggctga agggtcagga cgcaatcccc gccgccggcg tcaatccgca
     3961 ccggggccag cagttcccgg agttccacgc cttcttcgcg ggcgctctgc acctcctcgg
     4021 tgaaggcggg catgtcctcc cggcggcgtc ggtaaacgat gatggccctg cctccccgcc
     4081 ggataatgct gcgggcgacg tcgatggcgg tattcccccc gccgatgacg gcggagaggc
     4141 ccggtacgtc tcgcttgact cccccccgga tctcccggag gaaggagagg ccgtcttcca
     4201 ccccgggacc cgccgcgccg gggatgtcca ggggccggtc gcgccagtag ccgcagccga
     4261 ggaacagggc gtcgtaggat ttgcgcattt cttcaaaacg ggcggccgag acggcctcgc
     4321 cggtgcggat gcggacgcct tgggcctcga tgcgggcgat ttcggcgttc aggaccgcct
     4381 cggggagacg ataggcgggg atgccccagc gcagcatccc gcccgcggcg ggcatggcct
     4441 cgaagatctc cgtttcgtag cccaggatat tgaagaacca ggcggcggaa agtccggcgg
     4501 gaccggagcc gataatggcg atctgccgtc ccgtcgccgg ttgccgcggc agtgccggtt
     4561 tggtctcgtt gcgcgcggcg gcgtcggcga ggaagcgttc cagggtgtga atggccacgg
     4621 cggcgtcgaa ctcccggcgg ttgcaccggc cctcgcaggg atggtagcac acccggccgc
     4681 aaacaccggg caggggattt tccatcagga tggtctccca ggcctctttg aaggccccct
     4741 gggtgatgag catctcgatc cgggcgatgt cctccccggc cgggcaggcg gcgctgcagg
     4801 gcgaggtctt ttcctcgtaa cggggccgga ggaaacgcca ggagccggtc ttgttgcttt
     4861 ccgtggtggt ggaggtccgg ggcatgtaaa ggatgttctg caaagcggtc ggtgtttcca
     4921 tatgcctcac ctatgatatc gaaccatgca aaattacttc attgtatgcg tcccgggcgg
     4981 cggcgatgtt tgcctccggg tccgaagggg cctcttcacg gatggcgccg caggccgcct
     5041 ccaggggcgg cattgccagc atccgggcga aggcgccgat catggccgta ttgacgatgg
     5101 gatgggtgcg gcttcccagg aggtggcgca aggcgatccg ggtggcgtcc acgcaggcga
     5161 gtttcatgcc ccggtagggg tccaggttct ccggaagggc cgggctgttg aggaggaccc
     5221 agccacccgg tttcatgccc cgggtgacgt cgatgttctg gagcagggtc tggtcctgca
     5281 ccaccacgtg atcgggggcc gtaacgttcg tccggacgag gatcttcttt ttgtccaggc
     5341 gcaagtaggc ctccaccggg gcgccgcgtc gctccacacc gaaaaagggg aagctctgta
     5401 cctcccagcc ggcctggaag aaggccttgg agagcaggat cgtggccacc accgtgccct
     5461 gtccgccccg tccatgaaac cggatttcca gcatcttttc gcctcccatc ctt
//
LOCUS       DAOSMF010000124         5492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_16248_length_5492_cov_22.249402, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000124 DAOSMF010000000
VERSION     DAOSMF010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5492
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16248_length_5492_cov_22.249402"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="PLT63_09455"
     CDS             complement(<1..751)
                     /locus_tag="PLT63_09455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOD98988.1"
                     /translation="MTTYERGKLYEISIIDFKLDPDQPRKVIDPDALAELTASIKTHG
                     ILQPLLFRQGDQGWLYIVSGERRYRAAKELGLLTLPAMLVEGNHAEIALVENLLRQDL
                     TAVEEAEALQRLKDDQQYTDEQLSGVIGRARTTLSDILLINRLPAEIRDECRGDRKVS
                     KSALIEIARKKQARAMLTAWTKYKEKQAKAAAGATRKEKAPPTPEELIDWVTKANTKL
                     NAIDPAAWTEEEKTALAEALEDLQETVGGLLL"
     gene            complement(831..1979)
                     /locus_tag="PLT63_09460"
     CDS             complement(831..1979)
                     /locus_tag="PLT63_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98989.1"
                     /translation="MDIEFHYYITYLIAARAGLPPPEAVTLAWASQHTDDNTFMCTVD
                     AGRPTEYRNYISQTADILKPRLDLMRIYSLFHFLPGDPQAPGAWRKDGAMHWLTTTPG
                     SDNANDLLAAALATGNLYRIGIAAHAFADTWAHQNFVGYANPFNAFLKEDVATAIMPN
                     VGHADAFFAPDEVDRRWEDPRLIHGPIDNRARFLAAADDLYRKLARHWDPALPPEELS
                     RRAASLRDDLGRCFATSSPDPTAALAASAPDPPAATPATSFDVLRPGVLDPAEVARRA
                     QEERLSRYRALAAQVTYGGRDIPPYDPDRWMDEALHEQVHGLRDRSDFILSCLDLWPD
                     VFTWRDRAGDAYRQTPWYRFQEAVKAHQRETWQLLANRNFLALELPAF"
     gene            complement(2159..3367)
                     /locus_tag="PLT63_09465"
     CDS             complement(2159..3367)
                     /locus_tag="PLT63_09465"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD98990.1"
                     /translation="MRFERKILKALRERIDTPEIVVVTGMRRVGKTTALRMIHDEIPS
                     RNKAFIDIENPIDQRIFEEKDFNNIWANLQAYGISAREKAYLFLDEIQAMPDIVKAIK
                     YLHDHDADGAPAPPQVKFFLTGSSSYYLKNLFPESLAGRKRIYELNPLDYEEFLTFKG
                     HPRPFSDTFAAKERDKNAIRFEKERKLYDEYLTWGGFPQVVLTDDPRDKGAQLNDILK
                     SYFEKDVRLISDFRQINHFRDLLLLLLQRAGLRLDVTKLASEASLSRDTVYSYLAFLQ
                     GTYVINLIPPYTLNRDREISGAKKVYICDNGFLNYFGKTDEGALLENGVYLNLRKYGE
                     IRYYQRRTGRELDFILPEMQTGIEVKQTGDARDIRRVAALAQGLNLKEQYVVTREFRD
                     LPGLIPAQDL"
     gene            complement(3547..4200)
                     /locus_tag="PLT63_09470"
     CDS             complement(3547..4200)
                     /locus_tag="PLT63_09470"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOD98991.1"
                     /translation="MDRVLSLLRAAESSFYLTGGTALGRHYLHHRYSDDLDLFVNQAD
                     DFRDQVKKALEVLRQSGIAFAAGTAADDFVRILARRDETPLKVDFVNDVAFHYGDLQE
                     AAFYPRIDHWRNILSNKLCALSRREPKDMADIHSIARHFPFAWPEIFGEARQKDLWVE
                     PIEISRMIQEFPIDLLAAVKWVQPIDPGLIAADLKVIHRDIFHGQANSLVSPAPAKR"
     gene            complement(4214..4504)
                     /locus_tag="PLT63_09475"
     CDS             complement(4214..4504)
                     /locus_tag="PLT63_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98992.1"
                     /translation="MDPEIDITAKLADVFWDMDVHPDRLLRLLRGDIEKIGHVDRANL
                     FRRLLTTYDWYTLLAMIPPDRLPEALSDAVLDHLHPAGLKDRYRYARRILYG"
     gene            complement(4529..4972)
                     /locus_tag="PLT63_09480"
     CDS             complement(4529..4972)
                     /locus_tag="PLT63_09480"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HOD98993.1"
                     /translation="MVDRTLILRKLSQLEEYLGQLREFSTVSVEEYAADWKIQRIVER
                     TLQMMIELCLDIAGHIIADKKLRAPVTYADTFKSMFEAGLINSELLEIMEKMAKFRNV
                     VVHQYENVDTAIVMMILRKHLADFLLFRDAILKIIYDESPPQTKR"
     gene            complement(4965..5372)
                     /locus_tag="PLT63_09485"
     CDS             complement(4965..5372)
                     /locus_tag="PLT63_09485"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOD98994.1"
                     /translation="MKNANFKDLTPEISKIFDEDERVKFAYLFGSVARKEQNIHSDID
                     IAVYLEDDCDFIEAKLELIGTLMEHFGTDNLDLVILNTAALPLAARVIRDGAVLAERD
                     RFARHRYESRILREYFDFSILETSILNRRYGLG"
BASE COUNT         1074 a         1457 c         1764 g         1197 t
ORIGIN      
        1 ccaacaagag ccccccgacc gtttcctgga ggtcttccag ggcctccgca agggcggtct
       61 tttcttcttc ggtccaggcg gcggggtcga tggcgttgag cttggtgttg gccttggtca
      121 cccagtcgat gagttcttcc ggggtggggg gggccttttc cttgcgcgtg gcgccggcgg
      181 cggctttggc ctgtttttcc ttgtacttgg tccaggcggt gagcatggcc cgggcctgtt
      241 tcttccgggc gatctcgatg agggcgctct tggagacctt gcggtcgccc cggcattcgt
      301 cccggatctc ggcggggagg cggttgatga ggaggatgtc cgagagggtg gtccgggcgc
      361 ggccgatgac gccgctcaac tgctcgtcgg tgtactgctg gtcgtccttg aggcgctgga
      421 gggcctcggc ctcttccacg gcggtgaggt cctggcggag gaggttctcc acgagggcga
      481 tctccgcgtg gttcccctcc accagcatcg ccggtagggt caaaaggccc aactccttcg
      541 ccgcccggta gcgccgctcc ccggagacga tgtagagcca cccctgatcg ccctgacgga
      601 agaggagggg ctggagaatc ccgtgggtct tgatggaggc ggtcagctcc gccagggcat
      661 cggggtcgat gaccttccgg ggctggtcgg gatcgagctt gaagtcaatg atggaaatct
      721 cgtagagctt gccgcgttcg taagtcgtca tggttttcac ctcgctgatg gttacatgaa
      781 aacgttttta tggggtcagg tcttgaaaat tagcgttttt cggggggcga tcaaaacgcc
      841 ggcagctcca gggcgaggaa gttgcggttg gccaggagtt gccaggtctc gcgctggtgg
      901 gccttgacgg cctcctgaaa ccggtaccag ggagtctggc ggtaggcatc cccggcgcgg
      961 tcgcgccagg tgaagacatc cggccagagg tccaggcagc tcaggatgaa atccgagcgg
     1021 tcgcggaggc cgtggacctg ctcgtggagc gcctcgtcca tccagcgatc agggtcgtag
     1081 ggcgggatgt cgcggcctcc gtaggtgacc tgggcggcga gggcgcgata ccgggatagg
     1141 cgctcctcct gggcgcggcg ggcgacttcc gccggatcca ggactcccgg gcggaggacg
     1201 tcgaaggagg tagcgggggt ggccgctggc gggtcaggtg cggaggcggc gagggccgcc
     1261 gttgggtcag gtgaggaggt ggcgaagcac cgccccaggt cgtcccggag ggaggcggcg
     1321 cggcgcgaca gctcttccgg aggaagggcc ggatcccaat ggcgggccag cttccggtag
     1381 aggtcatccg cggcggcgag gaaccgggcc cggttgtcga tgggcccgtg gatgagccgg
     1441 gggtcttccc accgccggtc cacctcgtca ggggcgaaga aggcatccgc atggcccacg
     1501 ttgggcatga tggcggtggc gacgtcttcc ttcaggaagg cgttgaatgg attggcgtag
     1561 cccacgaagt tctggtgcgc ccaggtgtcc gcgaaggcgt gggcggcgat cccgatgcgg
     1621 tagaggttcc ccgtggccag ggcggccgcc aggaggtcgt tggcgttgtc gctccccggg
     1681 gtggtggtga gccagtgcat cgccccgtcc ttgcgccagg cgccgggggc ctgggggtcc
     1741 ccggggagga agtggaacag ggagtagatc cgcatcaggt cgaggcgggg cttaaggatg
     1801 tccgcggtct ggctgatgta gttccggtat tccgtggggc ggcccgcatc gacggtgcac
     1861 atgaaggtgt tgtcgtccgt gtgctgggac gcccaggcca gcgtcacggc ctccgggggc
     1921 ggcagccccg cccgggcggc gatcagatag gtgatgtagt agtggaattc gatatccatg
     1981 cgatcttggg ggtcaggtct taaaaattag cgtttttcgg gggatgcatc cgggcgcgtt
     2041 atgacgtagc cgcccgtctt aaacaatgat agatatcaag ctgcggctca tgaaatccac
     2101 tgccatagca tggttacatg cgcccaatga tatccccaat ttttataggt attagccgtc
     2161 acagatcctg ggcggggatc aggccgggga ggtcgcggaa ctccctggtg acgacatact
     2221 gctccttcag attcaggccc tgggcaaggg cggcgaccct ccgtatgtcg cgggcatccc
     2281 ccgtctgctt gacttcgatg cccgtctgca tctcggggag gatgaagtcc agctcacgtc
     2341 cggttcgccg ctgataatac cgaatttctc catatttgcg caggttcaga tagacgccgt
     2401 tctccaggag ggcgccttcg tcggtctttc cgaaataatt caggaaaccg ttgtcgcaga
     2461 tataaacctt tttggcgccg ctgatttcac ggtcgcggtt gagggtgtag ggagggatca
     2521 ggttgatgac gtaagtgccc tgcaggaagg cgagataaga ataaacggta tcccgggaaa
     2581 gactcgcctc ggaggccaac ttggtcacgt ccaggcgcaa cccggcccgc tgcagcaaca
     2641 gcagcaacag atcccggaaa tggttgatct gccggaaatc ggagatcagc cggacatcct
     2701 tttcaaagta ggatttgagg atgtcgttga gctgagcgcc cttgtcgcgg ggatcatccg
     2761 tgaggaccac ttggggaaag ccgccccagg tgagatattc gtcgtagagt ttccgttcct
     2821 tctcgaagcg gatggcgttt ttgtctcttt ccttggcagc aaaggtatcg gaaaagggcc
     2881 ggggatgacc tttaaaggtc aggaattcct cgtaatccaa cggattgagt tcataaatgc
     2941 gcttgcggcc tgccaggctc tcgggaaaga ggttcttgag ataatagctg ctggaaccgg
     3001 taaggaagaa tttgacctgc gggggcgcag gggcgccgtc cgcgtcatgg tcgtggagat
     3061 acttgatggc tttgacgatg tcgggcatgg cctggatctc gtccagaaac aggtaggcct
     3121 tttcacgggc ggaaatgccg taggcctgga ggttggccca gatgttgttg aaatcctttt
     3181 cttcgaagat ccgctggtcg atggggtttt caatgtcgat gaaggccttg ttgcggctgg
     3241 ggatttcgtc atggatcatc cgcaaggcag tcgtcttgcc gacacggcgc atccctgtga
     3301 caacaacgat ctccggcgtg tcgatccgct cgcgcagggc cttgagaatt tttctttcga
     3361 atcgcatgcc agcagcctac atattaatta tcaagatgtc aagtgctaaa cgacagatta
     3421 agtgatttga taaatggggg cagagaccat atttactgct gaattacccc tgatcactcc
     3481 gggtagttat gcaaagattt ccgtgatata gcgagctgga tgggcttttt ccgcaggtct
     3541 ttaaaattag cgttttgcgg gggcggggga gacgagggag ttcgcctgtc catgaaagat
     3601 atcccgatgg atcaccttaa ggtcagcggc aataaggccc ggatctatcg gttgcaccca
     3661 tttgaccgcc gccagcagat caatgggaaa ctcttgaatc atcctggata tttcaatcgg
     3721 ttcgacccag agatccttct gccgggcttc gccgaagatc tccggccagg caaaggggaa
     3781 atgccgggcg atggagtgga tatcggccat gtccttcggt tcccggcggg aaagggcgca
     3841 aagtttgttg gaaaggatgt tccgccagtg atcgatccgg ggataaaaag ccgcttcctg
     3901 aagatcgccg tagtgaaatg ccacgtcatt gacgaaatcg accttgaggg gcgtctcgtc
     3961 ccggcgggcc aggatacgga caaagtcgtc cgcggcggtc cccgccgcga aggcgatccc
     4021 gctttggcgc aggacctcca gcgccttttt gacctgatcc cggaaatcgt ccgcctgatt
     4081 gacgaacaga tcgaggtcgt ccgaatagcg gtgatggaga tagtgcctgc cgagggctgt
     4141 cccgccggtc aggtagaatg acgactccgc cgcccggagc agtgacaaga cccgatccat
     4201 gaacgggtaa actttatccg tatagaatgc gtcgggcata gcgatagcga tccttgaggc
     4261 ccgcgggatg gagatgatcc aaaacggcat cgctgagggc ctcagggagg cgatccggcg
     4321 ggatcatcgc cagcagtgtg taccagtcat aggttgtgag cagtcggcga aagaggttgg
     4381 cgcgatcgac atgcccaatc ttctcgatat cgccgcgcag gagtcggagg agccgatcgg
     4441 gatggacatc catatcccag aaaacgtcag ccagtttggc ggttatgtct atttcggggt
     4501 ccatatttcg ggggtcaggt ctttaaaatt agcgttttgt ctggggtggc gattcatcat
     4561 aaatgatttt cagtatggca tctcggaaga ggaggaaatc ggccagatgc ttcctgagga
     4621 tcatcatcac gatggccgta tcgacgtttt cgtactgatg aacgaccaca ttacgaaact
     4681 tggccatttt ctccattatt tccagcagtt cagaattgat caggccggct tcgaacatgc
     4741 ttttgaatgt gtcggcgtaa gtgacgggcg cccggagctt cttatcggcg ataatgtgtc
     4801 cggcgatgtc aagacacaac tcgatcatca tctgcaacgt tctttccacg atccgctgga
     4861 tcttccagtc cgcggcatat tcctcgaccg aaacggttga gaattcccga agctgcccca
     4921 gatactcttc cagttgagaa agcttgcgca ggattagcgt gcgatcaacc aagcccatac
     4981 ctccggttca agatcgacgt ctccaggatg gaaaagtcga aatattccct gagaatgcgt
     5041 gattcgtagc gatgccgggc aaaacgatcc cgctccgcca ggaccgctcc gtcccggatg
     5101 acccgcgccg ccagcgggag agccgccgtg tttagaatca ccagatccaa gttgtctgtt
     5161 ccgaaatgct ccatcaatgt cccgatcagc tccagtttcg cctcgatgaa atcacaatca
     5221 tcctcaagat atacagcaat gtcgatgtca ctgtggatgt tttgctcttt gcgcgccaca
     5281 ctgccaaaaa gatatgcgaa cttcacccgt tcatcttcat caaaaatttt cgatatttcg
     5341 ggggtcaggt ctttaaaatt agcgtttttc aactttcacg tgccaggcgc cgggcgttct
     5401 ttaattaaac ttccgcggca atacctgtcg atacgcgcgg ccaattccaa cagctccggg
     5461 acgctggcca agacttcgga aaagagggcc gc
//
LOCUS       DAOSMF010000125         5488 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_16264_length_5488_cov_22.315664, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000125 DAOSMF010000000
VERSION     DAOSMF010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5488
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16264_length_5488_cov_22.315664"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..617)
                     /locus_tag="PLT63_09490"
     CDS             complement(69..617)
                     /locus_tag="PLT63_09490"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD98995.1"
                     /translation="MLELALHILDITENSTRAGARRIDMNLEENHEADLLTLEISDDG
                     AGMDPETLSKALDPFYTTKKVRRVGLGLPMLAQAAERTGGGLTLTSEPGRGTRLKVTF
                     GLSHLDRQPLGDLPGVITTLIAGNPDIEFNYHHRCGDRVFALKTEDLKRELEDVPINH
                     IEVLKYIRQYVKDGLQDIVSEA"
     gene            complement(619..1356)
                     /locus_tag="PLT63_09495"
     CDS             complement(619..1356)
                     /locus_tag="PLT63_09495"
                     /inference="COORDINATES: protein motif:HMM:NF024660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HOD98996.1"
                     /translation="MLKAMRCDLHIHTCLSPCAELDMYPRAIVRRALEKKLDIIGICD
                     HNASENAVYVQRAAAGLPLAVLPGMEVASREEVHTLALFENIIRLRHFQELVYGHLEG
                     ENDEEVFGCQAIVNEYDEVEGFNGRLLIGATDLPLQEIIAGIHGEGGIAIAAHVDRES
                     FSVIGQLGFIDPSFAFDALEFTPRTGLGKARQCHPDLARYPFISSSDAHFIGDIGRGW
                     TEMLLAEASFREVRLALAGGKGRRING"
     gene            complement(1402..1764)
                     /locus_tag="PLT63_09500"
     CDS             complement(1402..1764)
                     /locus_tag="PLT63_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD98997.1"
                     /translation="MNLGAIVKELDLKVLSGRDRLDAEVTGGYVGDLLSDVMANSKEG
                     DVWLTRQIHQNIVAVASLKDHAGVIIIQGAEPAADTLEKARKEGIPIMVSAMPGFETI
                     GRVFNMITAKKSLEASDR"
     gene            complement(1780..2235)
                     /locus_tag="PLT63_09505"
     CDS             complement(1780..2235)
                     /locus_tag="PLT63_09505"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD98998.1"
                     /translation="MLSHDTAIYENSFSVEGGTFDNAGHVSSQIKALLKKMKLSKEVV
                     RRAALVTYEAEINICSYADRGKIHLRVTPEHVTVEAIDEGQGIVDIELAMQAGYSTAT
                     ERIREMGFGAGMGLGNIKNFSDFFRITSEIGKGTCLKMVIRIDKDFDGI"
     gene            complement(2222..2596)
                     /locus_tag="PLT63_09510"
     CDS             complement(2222..2596)
                     /locus_tag="PLT63_09510"
                     /inference="COORDINATES: protein motif:HMM:NF018753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HOD98999.1"
                     /translation="MTLREVKDILDAEVLVGYDQLDKEVKTAFGADLMSDVLAFAKAG
                     SLLLTGLTNPQVIRTSDVLDIAAIIMVRGKRPSPETIRLAEELLIPILVTKYILFETA
                     GKLYAKGIVGCVERVDDERAFS"
     gene            2847..>5488
                     /locus_tag="PLT63_09515"
     CDS             2847..>5488
                     /locus_tag="PLT63_09515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOD99000.1"
                     /translation="MGDDMQERKFDPEGRQAVVLAEEEAVHRSEEKCAQAEAALRSME
                     ESYRHLVDNATDIIYRTDATGHFTLFNPAAIKITGYSEAELMHRHFLELVHPDHRAAA
                     ERLYGRQYLKKTASTYFEFSLRRKDDSEVWLGQHVQLVMEGDDIIGFHAIARDISQQK
                     KLEAELQDSEHRLNMILGAVQAGIVLVDARTHVIEDANKSAVQMINVPKEEIVGRVCH
                     RFICPAEEGRCPITELGRQIDNSERILLTASGAEVPILKTVVPIVIKNRDYLIESFVD
                     ISKLKRAEEEAKRENAKLSTMISGMEEGVVFADAEDVIVAANDYFCRFIGVTHQEIVG
                     RDLHSFHASPVREKVADSLRKFREHPGATAVIMNKSLRDSHVILRMQPVYREGIYDGV
                     LLNIVNVTDLVEARLKAEEASRAKSEFLSTMSHEIRTPMNAIIGMAELLAETPLTEEQ
                     RKYVEVFRAAGENLLVIINDILDLSKVEAGHVHLETVDFELSQVVERTCDVMAQRAHD
                     KGLELLHHKDYDVPEQLRGDPGRLQQLLINIIGNALKFTEKGEVMLRIKMAEEECLLP
                     DPDQVALMFSVSDTGIGIPADKLGIIFEKFTQADASTTRKYGGTGLGLAISKRLVELM
                     GGRIWIESEPGRGTKVHFTARFALPGDVKAGPAPVEESLAGVKILVVDDNETNRLILR
                     GMLGRWEAAAMDVEDGARGYEELKRAVASGSPYRLVLMDYQMPEANGFETAARITGDP
                     DLRHVPIIILSSGYCLDRPEHMERYGVSGFIQKPVKMAELRAAISRVLQPGRESGPQS
                     GPPAPAATAAARPLHILLVEDNEDNRLLFRSFLKGGPHSIQEAENGLEALEAFKNAGK
                     RFDLIFMDMQMPVMDGYTATAAIRR"
BASE COUNT         1189 a         1565 c         1586 g         1148 t
ORIGIN      
        1 taaattcctt cagtaactcc tctttttcaa gcttcatgaa tatcttcctc ctaagtcgaa
       61 aagcgcattc acgcttcgga tactatgtcc tgcagtccat ctttaacata ctgccttatg
      121 tatttcagca cctcaatgtg attgatggga acgtcttcca gttccctttt taagtcctcc
      181 gtcttcaggg caaaaacccg gtccccgcaa cggtgatgat aattgaattc gatgtcgggg
      241 ttgccggcga tcagggtcgt gatgacgccg ggaagatctc ccagcggctg gcgatccaaa
      301 tgactcaacc cgaaggtaac cttcaggcgg gtgccccggc ccggttccga cgtcagcgtc
      361 aggccgcccc ccgttctttc cgccgcctgg gccagcatgg ggagccccag gcccacccgg
      421 cggacctttt tggtggtgta aaaggggtcc agggccttgc tcagggtctc cgggtccatc
      481 ccggcgccgt cgtcgctgat ctccagggtg aggagatccg cttcgtgatt ctcctccagg
      541 ttcatgtcga tgcgacgggc ccctgcccgc gttgaattct cggtgatatc gaggatatgc
      601 aatgccagct ccagcatgtc aaccgtttat tcttcggcct ttcccgccgg ccagggccag
      661 gcgcacctcc cggaagcttg cctcggccag cagcatctcc gtccagcccc ggccgatgtc
      721 gccgataaaa tgggcatccg aagaggagat aaagggataa cgggccagat cggggtggca
      781 ctgccgtgcc tttcccaagc ccgtccgggg ggtgaactcc agggcgtcaa aggcgaagga
      841 gggatcgatg aaccccaact gcccgatgac gctgaaactc tcacgatcca cgtgggcggc
      901 gatggcgatg ccgccctccc cgtggatacc ggcgatgatc tcctgcaacg gcagatcggt
      961 ggccccgatg agcagccggc cgttgaatcc ttccacttcg tcgtattcgt tcacgatggc
     1021 ctggcagccg aagacttcct catcgttttc cccctccagg tgtccataaa ccagttcctg
     1081 aaaatgccgc aaccggatga tgttttcaaa gagcgccagg gtgtgcacct cctcccggct
     1141 ggccacctcc attcccggca ataccgcaag cggcagtccg gcggcggcgc gctgaacgta
     1201 aacggcattc tccgaggcat tgtggtcgca gatgccgatg atgtccagct ttttctccag
     1261 ggcccggcgc actatggccc gggggtacat gtccagctca gcgcatggtg aaaggcaggt
     1321 gtggatgtga aggtcgcaac gcatcgcctt caacatgggc cgttcctaaa aatacaaaaa
     1381 aaagaccatg gctacatggt ctcatctgtc cgatgcctcc aacgacttct tcgcggtgat
     1441 catattgaat accctgccga tcgtttcaaa gccgggcata gctgacacca tgatgggtat
     1501 cccttctttg cgggccttct ccagggtgtc ggcggccggt tcagccccct ggatgataat
     1561 gacgcccgca tggtccttca gactggccac cgccacgatg ttctggtgga tctgccgggt
     1621 gagccacacg tctccttcct tgctgttggc catgacgtcg ctcaaaaggt cgccgacgta
     1681 tccgccggtg acctcggcat caagccggtc ccgacccgac aggaccttca aatcgagttc
     1741 tttgacgatc gcgcctaaat tcatggttaa gagagttctc taaatgccgt cgaaatcctt
     1801 gtcgatgcga atgaccattt tcaggcaagt gcctttgccg atttccgaag tgatccgaaa
     1861 gaaatcggag aagtttttga tattgcccaa ccccatcccg gcgccgaaac ccatttcccg
     1921 aatccgctcc gtggccgtgg aatagccggc ctgcatggcc agctcgatgt ccacgatccc
     1981 ctgaccttcg tcgatggcct cgacggttac gtgttccggc gtcacccgga ggtggatctt
     2041 gccccggtcg gcataggaac agatgtttat ctccgcttca tatgtaacta aagccgccct
     2101 gcggacaacc tccttggaca gcttcatttt tttcaacagg gccttgatct gactggaaac
     2161 atgcccggcg ttgtcaaagg tgcctccttc tacggagaat gagttttcat agatggccgt
     2221 gtcatgagaa agcacgttcg tcgtcaaccc tttccacgca gccgacgatc ccttttgcgt
     2281 acaacttccc ggcggtttcg aagaggatgt attttgtgac caggatgggg atcagcagct
     2341 cttccgcaag acggatcgtt tccggggaag gacgtttgcc acgtaccatg atgatggcgg
     2401 cgatatcgag cacgtcggat gtccggataa cctgtggatt cgtgagcccg gtaagcaata
     2461 gacttcccgc cttggcaaag gccagcacat cactcatgag atcggccccg aaagcggttt
     2521 tcacctcttt gtccaattga tcatagccga cgaggacttc agcatccaga atatctttca
     2581 cctctcgaag ggtcaccttg gtctccttta ctgaaagaat agtgttaaaa aacaggctca
     2641 tattagctaa tcgtcatgcc agaggggatt accataaggg cgggggggat gtcaatagaa
     2701 ttgtccccgc gctggcggtg aaaataattg actcccttct gaagcccttc atgatataat
     2761 aaaaaccagg ctggggcagt cggcgcccct attaacaaat caccgggggt gaaatatctc
     2821 ctgaagtacc cggggaaagg aacggcatgg gagacgatat gcaggagcgg aaattcgatc
     2881 cggaggggcg gcaagccgtc gtccttgccg aagaagaagc cgtccaccgg tccgaagaaa
     2941 aatgcgccca ggcggaggcg gcattgcgga gcatggagga gtcttaccgg catctcgttg
     3001 acaacgcgac ggacataatc taccggaccg acgccaccgg ccattttacc cttttcaatc
     3061 ccgcggcgat taagattacc ggttattccg aggcggagct gatgcacagg cacttcctcg
     3121 aactggtcca tcccgatcat cgggccgctg ccgagcgact ctacggaaga caatatctca
     3181 aaaagacggc cagcacctat tttgagtttt ccctgagaag aaaggacgac agcgaggtct
     3241 ggcttgggca gcacgtgcag ttggtaatgg aaggtgatga tatcattggc ttccatgcca
     3301 ttgcccggga tatttcccaa cagaagaaac tggaggcgga gctccaggat tcggagcatc
     3361 gcctcaacat gatcctgggg gccgtacagg caggcatcgt cctcgtggac gcccggaccc
     3421 atgtcatcga agacgccaat aagagcgccg tgcagatgat caacgtcccc aaggaggaga
     3481 ttgtcgggcg ggtttgccac cgctttatct gtccggcgga ggagggcagg tgtcccataa
     3541 cggaattggg tcggcagatt gacaattcgg agcgcatcct ccttacggcg tccggagcag
     3601 aagtgccgat cctgaaaaca gtggttccca tcgtcatcaa gaaccgtgat tatctcatcg
     3661 agagctttgt ggatatctca aaactgaaaa gggcggaaga ggaggccaaa cgtgagaacg
     3721 ccaaactttc caccatgata tccgggatgg aggaaggagt ggtgtttgcc gacgccgagg
     3781 atgtcatcgt ggcggccaat gattatttct gccgttttat cggtgtcacc caccaggaga
     3841 ttgtcggccg ggacctccat tcctttcacg cttcccccgt ccgggaaaag gtcgccgatt
     3901 ccctgcgcaa attccgggaa catcccggcg ccacggccgt gatcatgaat aagtccctca
     3961 gggacagcca tgtgatcctc cgcatgcagc cggtttaccg ggaagggatt tacgacgggg
     4021 tccttttgaa catcgtcaac gtcaccgatc tggtggaagc ccgcctcaag gccgaggagg
     4081 ccagccgggc caagagcgag tttctctcca ccatgagcca cgaaatccgt acgcccatga
     4141 acgccatcat cgggatggcg gagttgctgg cggaaacgcc gctcacggag gaacagagaa
     4201 aatacgtgga ggtatttcgg gcggccgggg agaatcttct ggttatcatc aatgatatcc
     4261 ttgacctctc gaaggtggag gcgggacatg tccacctgga gacggtggat ttcgaactct
     4321 cccaggtggt ggagaggacc tgcgacgtca tggcccaacg ggctcatgac aaggggctgg
     4381 agctcttgca tcataaagac tacgatgtgc cggagcaact ccgcggcgat ccgggtcggc
     4441 tccagcaact gctcatcaat atcatcggca acgccttgaa attcaccgaa aagggcgagg
     4501 taatgctgag gataaaaatg gccgaagagg agtgcctcct tcccgatccc gatcaagtgg
     4561 cgctgatgtt ctccgtgtcc gatacgggga tcggcattcc cgcggacaaa ctggggataa
     4621 tcttcgagaa attcacccag gccgacgcct cgacgacgcg caaatacggg ggtaccggcc
     4681 tgggcctggc gatctccaaa aggctggtgg agctcatggg gggacggata tggatcgaga
     4741 gcgagccggg gcgggggacg aaagtccact ttaccgcccg tttcgccttg ccgggggacg
     4801 tcaaggccgg accggcaccg gtggaagaat cactggcggg cgttaagatc ctggtggtgg
     4861 acgacaatga gaccaaccgg ttgattctcc gggggatgct cggtcgctgg gaagcggcgg
     4921 cgatggatgt cgaggacggc gcccgcggtt atgaggaatt gaaaagggcc gttgcgtcgg
     4981 gttctcccta ccgcctggtc ctcatggatt accagatgcc ggaggccaac ggtttcgaga
     5041 ccgcggccag aatcaccggg gatccggatt tgcgccatgt ccccatcatt atcctgtctt
     5101 cgggttattg tctggaccgt ccggaacata tggaaagata cggggtgtcc ggattcatcc
     5161 agaagccggt gaagatggcg gaattgcggg ccgccatcag cagggtcctg caacccggac
     5221 gggaatcagg accgcagtcc gggccgccgg cgcccgcggc gacggcggcg gcgagaccgt
     5281 tgcacatcct gctggtggag gataatgaag acaaccgtct tctcttccgt tccttcctca
     5341 agggcggccc ccactccatc caggaggccg agaacggtct ggaggccctg gaggccttca
     5401 agaacgccgg gaaaagattc gatctcatct tcatggacat gcagatgccg gtcatggacg
     5461 gctatacggc gacggcggcc atccgccg
//
LOCUS       DAOSMF010000126         5461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_16367_length_5461_cov_17.837588, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000126 DAOSMF010000000
VERSION     DAOSMF010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5461
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16367_length_5461_cov_17.837588"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="PLT63_09520"
     CDS             complement(<1..373)
                     /locus_tag="PLT63_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOD99001.1"
                     /translation="MTVAQMKDRLIEIIIAKSFQYRDHPPFTLASGKTSNYYFNCKPT
                     TLDPEGMNLIGGILFRMLADTEITAAGGLTLGADALANALAVISYQHGKSIKAFIVRK
                     DVKDHGTKSALEGDVKAGERVV"
     gene            complement(515..2953)
                     /locus_tag="PLT63_09525"
     CDS             complement(515..2953)
                     /locus_tag="PLT63_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOD99002.1"
                     /translation="MDKKQIKAMLKKRILILDGATGTELQKRGMPGGVCPEAWCAQHH
                     QVLQEVQRAYREAGADIIYTCTFGANRLKLGEYGLTDVRDVNERMARLARRAAGPGAL
                     VAGDIGPTGRFIEPFGDLPFEEAVAVFKEQVAGLLAGGVDLFVIETMMDIQEARAALL
                     AVKESCAHFTIVTMTYDTSGRTLNGTDPVTALITLQSLGADAVGGNCSAGPEDMQDWI
                     AAMKPYATVPLAAKPNAGLPRLANGITHFDMGAAEFASWGIPFHGAGVNLLGGCCGTT
                     PEHIAAIRAAMAGLSPEPPRRRSLAAVSSARTHLLLEGKQPLFVIGERLNPTGKKQLQ
                     QELREGKMSLVRRVAREQEDQGADLLDVNVGVPGIDEPPAMREVIRAVTAAAALPLVI
                     DSPKMATIEAALRLYPGRALINSISGEREKVEKLLPLAAKYGAMFILLPLADGEIPAT
                     AAGRAAIVQEVYRAARRFGYTKEDIVVDGLVMTVAANPEAPVEALKTVAWASRTFRCR
                     TVLGVSNVSFGLPERKWLNAAFLVLAQGVGLSMAIVNPAGPELQAVMKAADVLMSRDR
                     QAARYIAAFSGAGSGSEGASPPPPGKETTPAERVYQAILEGNRDDIVALIEAALASVA
                     AGELVNRQMIPAIMRVGEHYEKQTYFLPQLMASAEAMKRGLARLEPLLAASADRPAGK
                     GRIILATVQGDIHDIGKNIVALMLRNHGYEVIDLGKDVAAAEILAAMKKHRPQVVGLS
                     ALMTTTMTAMPDVIGAARREGLAVPFILGGAVVTAAYAESFGAAYARDSVDAVRVIGG
                     LLMEDEKKRLQTDK"
     gene            complement(3120..3740)
                     /locus_tag="PLT63_09530"
     CDS             complement(3120..3740)
                     /locus_tag="PLT63_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99003.1"
                     /translation="MTPLFFQSINVDLPRTAIYRRLGYRRNATRLDTARHREIEKYLG
                     EAGELITLRGTARRLPCWIEPPGRVVLDETTVFASRNLARFLKDAGETLLMGATAGAD
                     VVAAIQADMAGGNATRGVVLDAAASEITDAALDWIMSYFRQTLRREGKALLTNRFSPG
                     YGDFVLENQLIIHRLLPMERLGVTLTKTCLMVPEKSVTAVTGIVAP"
     gene            complement(3820..4782)
                     /locus_tag="PLT63_09535"
     CDS             complement(3820..4782)
                     /locus_tag="PLT63_09535"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOD99004.1"
                     /translation="MEKIAANFYMLTLPMPFRLGHVHAFLLVHDGRITLFDTGLNTPE
                     TFLHLSDSLRLIGKDIRDIERILITHYHIDHCGMAGRLQDLSGATIHMSMRSRPFIMT
                     QNGEERAFLDRIEFFCRRHGLPEKAIAFIGGLLGTFKKAGSSFIVTHFYEGGTKEIIG
                     KREMLVLPTPGHTHDHLSFYFPREGILLSGDHVLPEITPNLSPNLFAPDFRPLQSFLA
                     SLSRMEALSVATVYPAHGRPFADLKGRIAAIRSHHDARKNLTLTSVKEGPKTAFAVSR
                     DIFGSELNEFDQFLALNETYVHLVELVYEGLIREDRSGDSIVYA"
     gene            complement(5100..>5461)
                     /locus_tag="PLT63_09540"
     CDS             complement(5100..>5461)
                     /locus_tag="PLT63_09540"
                     /inference="COORDINATES: protein motif:HMM:NF012407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HOD99005.1"
                     /translation="IVTTNMFGDILSDEAAMITGSIGMLPSASLGERGGMYEPIHGSA
                     PDIAGRDLANPLATILSVAMMLHHSLGMPGEAEMVETAVRQVLRDGYRTKDIHHEGAI
                     LVGTAAMGDAVAARLLP"
BASE COUNT          977 a         1677 c         1737 g         1070 t
ORIGIN      
        1 cgaccacccg ctcgccggct tttacgtccc cttccagggc gcttttggtc ccgtggtcct
       61 tcacgtcctt gcggacgatg aaggccttga tggatttacc gtgctggtag gagatcaccg
      121 ccagggcgtt ggccaaggca tcggccccta gagtgaggcc tcccgcggcg gtgatctcgg
      181 tgtcggcgag catccggaag agaattcccc cgatgaggtt catcccctcg ggatcgaggg
      241 tcgtgggctt gcagttgaag tagtaattgc tcgttttccc cgaggcgagg gtaaagggcg
      301 gatgatcacg atattgaaaa gatttggcga tgatgatctc gatgaggcgg tccttcatct
      361 gggcaacggt catagcggta aacgactcct tcctgtttcg gtttcttcag cgcatcgtcg
      421 taatgtcggc gggcgggacg gcgtcggggc gaataggtcc cagcggtgaa ccgccttccc
      481 ggcgtccggc ccggagaacg gatgaactcg ctgattattt atccgtctgt aggcgtttct
      541 tttcgtcttc catgagcaac ccgccgatca cccgtaccgc gtcgacactg tcccgggcat
      601 aggcggcgcc gaaagactcc gcataagcgg cggtgaccac ggccccgccc aggatgaacg
      661 gcacggcaag tccctcccgg cgggcggcgc cgatgacgtc cggcatggcc gtcatggtgg
      721 tggtcatcag agccgacaac cccacgacct gagggcggtg tttcttcatc gccgccagta
      781 tctctgccgc cgccacgtct ttgcccagat cgataacttc gtaaccgtgg tttctcagca
      841 tcagggccac tatattcttg ccgatgtcgt ggatgtcccc ctgcaccgtg gccaggatga
      901 tccgcccctt gccggcggga cgatccgcgg aagccgcgag caggggttcc agacgggcca
      961 gccccctctt catcgcctcg gcgctggcca tcagttgcgg gaggaagtag gtctgctttt
     1021 cgtagtgttc ccctacccgc ataatggcgg ggatcatctg cctgttcacc agctcccccg
     1081 cggcgacgct ggccagggcg gcctcgatca aggcgacgat gtcatcccgg tttccctcca
     1141 ggatcgcttg atatacccgt tccgccgggg tggtttcctt tcccggcggc ggcggcgatg
     1201 ccccttccga cccggagcca gccccggaaa acgccgcaat gtagcgggcg gcctggcggt
     1261 cccggctcat gaggacgtcg gcggccttca ttacggcctg caactcgggg ccggcgggat
     1321 tgacgatggc catgctcagt cctaccccct gggccaggac aagaaaggcg gcgttgagcc
     1381 acttccgctc cggcagtccg aaggagacgt tggagacgcc caggaccgtc cggcagcgga
     1441 aggtacggga cgcccaagcc accgttttca gggcctcgac gggggcctcc gggttggccg
     1501 ctacggtcat gaccaggccg tccactacga tatcctcctt ggtatagccg aaacgccggg
     1561 cggcccgata gacctcctgg actatggcgg cgcgcccggc ggcggtggcg gggatctccc
     1621 cgtccgcaag cggcaggagg atgaacatcg ccccatattt cgccgccagg ggcaggagct
     1681 tttccacttt ttcccgttcc cccgagatgg agttgatcag ggcccgcccc ggatagaggc
     1741 gcagggccgc ctcgatggtc gccatcttcg gcgagtcgat gaccagcggc aaggcagcgg
     1801 cggccgtaac ggcccgaatc acctcccgca tcgccggggg ctcgtcgatg ccgggtacgc
     1861 ccacgttgac gtccaggagg tcggccccct ggtcttcctg ctcccgagct acgcggcgga
     1921 ccagggacat tttaccttcc cggagttcct gttggagctg cttctttccc gtggggttga
     1981 gccgttcgcc gatgacgaac agcggctgtt tcccctccag cagaagatgg gtccgggcgg
     2041 agctgaccgc agccagggag cggcgccggg gcggttccgg ggacaatccc gccatggcgg
     2101 ccctgatggc ggcgatgtgc tccggagtcg tgccgcaaca gccccccaga aggttgactc
     2161 cggcaccatg gaaggggata ccccaggaag cgaattccgc cgctcccata tcgaaatggg
     2221 ttatcccgtt cgccagacgg ggtaggcccg cattgggctt ggccgccagc ggtacggtgg
     2281 catagggctt catggcggcg atccagtcct gcatatcctc cggtccggcg gaacagttac
     2341 cgcccacggc gtcagccccg agactctgga gggtgatgag ggccgtaacg ggatcggtgc
     2401 cgttgagggt ccggccgctg gtatcatatg tcatggtgac gattgtaaaa tgggcgcagg
     2461 attccttcac cgccagcagg gccgcccggg cctcctggat gtccatcatg gtctcgatga
     2521 cgaaaaggtc caccccgccg gcgagcaggc ccgccacctg ttccttgaac accgccactg
     2581 cctcctcgaa gggaaggtcc ccgaaaggtt cgatgaaccg gccggtgggg ccgatatcac
     2641 cggcgacgag ggccccggga cccgcagccc ggcgggccaa gcgggccata cgctcattga
     2701 cgtcccggac gtcggtgagc ccgtattccc ccagtttgag gcggttggcc ccgaaggtgc
     2761 aggtgtagat gatatccgct cccgcttctc ggtaggcccg ctggacctcc tggaggacct
     2821 ggtggtgctg ggcgcaccag gcctcggggc aaacgccgcc gggcatcccg cgtttctgca
     2881 gctccgtgcc cgtagcgccg tcgaggatca ggatcctttt tttcagcatc gccttgattt
     2941 gctttttgtc caattctttc ctccccgctt tttgtcgctc cgccgcgcct gcctcggctg
     3001 ggtagtccgt ccttatagca cgccgcttga aaaaggcaat aaaaatccac gggtgcctgt
     3061 gttttggaaa gcttttccgc tccgccgcac cgggggagga cggaagcagc cgtcatggtt
     3121 cacggggcga cgatgcccgt tacggccgtc accgattttt ccgggaccat caggcaggtc
     3181 ttggtgaggg tcaccccaag ccgttccatc gggagtagcc gatggataat caattgattt
     3241 tccaagacaa aatctccgta acccggcgag aagcggttgg tgaggagagc tttcccctcc
     3301 cgcctgaggg tctggcggaa ataggacatg atccagtcca gagcggcatc agtgatctcg
     3361 ctcgccgcgg cgtcgagcac cacgccccgg gtcgcgttgc ctcctgccat gtcggcctgg
     3421 atcgccgcca cgacgtcggc cccggccgtg gcccccatga ggagcgtttc ccccgcgtcc
     3481 ttgagaaagc gggcaagatt ccggctggca aagaccgtcg tttcgtccag aacgacccgc
     3541 cccggcggtt cgatccagca aggcagccgc cgggccgtcc cccggagggt gatcagttcc
     3601 ccggcctcgc ccaaatactt ttctatctcc ctatgccggg cggtatcgag gcgagtggcg
     3661 ttccggcggt agccgaggcg ccgatagatg gccgttcggg gcagatcgac gtttatggac
     3721 tggaaaaaaa gcggcgtcat ggaacacatc cccagcggcg taccaagcga gcgacgcgat
     3781 cattcatcat cgggactccg aagcccattg gcatattcat catgcgtaaa cgatagaatc
     3841 tcctgatcta tcttccctga tcaacccttc gtaaaccagt tcgacaagat ggacataggt
     3901 ttcattgagg gccaggaact ggtcgaattc gttcagctcg gacccgaaga tgtcccggga
     3961 caccgcgaag gcggtcttgg gcccctcctt taccgacgtc agggtcaggt tttttcgcgc
     4021 gtcgtggtgg ctccggatcg ctgcgattct tcctttgagg tcggcgaagg gccggccgtg
     4081 ggcgggatag accgtagcga cggacagggc ttccatccgg gacagggacg ccagaaaact
     4141 ctgcaagggt cggaaatccg gggcgaagag attggggctg agattggggg tgatctccgg
     4201 caggacgtga tcccccgaca ggaggatgcc ctctcggggg aagtaaaaag agagatgatc
     4261 gtgggtgtgc cctggtgtcg gcaggaccag catctccctt ttccctatga tttccttagt
     4321 tcccccttcg tagaaatggg tgacgatgaa agagctgcca gcctttttga aggttcccag
     4381 caggccgcca atgaaggcga tggccttttc cggtaggcca tgacggcggc agaagaactc
     4441 gatacggtcc aggaaagccc tttcctcgcc attctgggtc atgatgaagg gccgactccg
     4501 catagacatg tggatggttg ccccggagag atcctggaga cggccggcca taccgcagtg
     4561 atcgatatga tagtgtgtga tcaggatccg ttctatgtcc cggatgtctt ttccgataag
     4621 gcgcagggaa tcgctgagat gcagaaaggt ctccggggtg ttgagccctg tatcgaacag
     4681 ggtgatccgg ccgtcgtgga caaggaggaa ggcatggacg tgaccgaggc ggaagggcat
     4741 gggcagggtg agcatgtaga agttggcggc gatcttttcc atggagggta agtttactcc
     4801 taaaaccggc gacgatcaag acatcttttc aataactgca cccgagttac tctctgataa
     4861 agcggccccg gcggggggac gtgggtcgct tctgtgcaac ttcacccgag aatgtagtgc
     4921 ggaccttcag gtccgccaac agcgcagcta aagctgcgca ctacaataat cagtgacaga
     4981 gtctcttcca agtaatttcg cttctgagca actgcactga taaagcgccc ccagggagcg
     5041 tgaatcgctt ctgacgaaat cgtaaaaggg ttgattgccc cgcctccgcc ggccgccgtt
     5101 caggggagga gccgggccgc cacggcgtcg cccatggcgg cggtgcccac caggattgcc
     5161 ccttcatgat ggatgtcttt cgtccggtag ccgtcccgta gtacctggcg gaccgccgtc
     5221 tcgaccatct ccgcctctcc gggcatgccc agggagtggt ggagcatcat ggccacggaa
     5281 agaatcgtgg ccagagggtt ggcgaggtcc cggccggcga tgtccggggc ggagccgtgg
     5341 atcggttcgt acatgccccc ccgctccccc aggctggccg agggaagcat gccgatggaa
     5401 ccggttatca tggccgcctc gtcgctgagg atgtcgccga acatgttggt cgtcacgatg
     5461 a
//
LOCUS       DAOSMF010000127         5457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_16381_length_5457_cov_20.129582, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000127 DAOSMF010000000
VERSION     DAOSMF010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5457
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16381_length_5457_cov_20.129582"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            241..519
                     /locus_tag="PLT63_09545"
     CDS             241..519
                     /locus_tag="PLT63_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99006.1"
                     /translation="MPPEMIAKIDTVLERVKDPESGLPVGQLGFIKRFRYNEKEGKLY
                     VFADTYRHLPKCVTCAAIAGAVMSGIIRDITTELQREFPDLAIEFAPA"
     gene            complement(906..1427)
                     /locus_tag="PLT63_09550"
     CDS             complement(906..1427)
                     /locus_tag="PLT63_09550"
                     /inference="COORDINATES: protein motif:HMM:NF025049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOD99007.1"
                     /translation="MAQKQDLGVFNFAIRAEKDGMNFYMKAAKKFSGNEDLKKLFVSL
                     AKEEAKHMDIFLEFKAKAEGKGIDQCLKNPDIDTYLEAIVQEGLFPKGDTVNKRLEKV
                     DTVAQAAAIAMAAEKNAILLYTELAKLAKDKDQKKFFEKIVKEEKSHLVMVAGLRADN
                     DPMYAALRFGRFF"
     gene            complement(1519..1839)
                     /locus_tag="PLT63_09555"
     CDS             complement(1519..1839)
                     /locus_tag="PLT63_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99008.1"
                     /translation="MRKLPVFIIFSLVLMLGVIMLAGCGEGGKQPPPSPTQQQGAATQ
                     EAPPAAAKPQSCYDVGFKIGRCAAKSMSNLPCEPGEDIPMPLECKGKPETKKGLAEGR
                     KSVY"
     gene            complement(1848..2507)
                     /locus_tag="PLT63_09560"
     CDS             complement(1848..2507)
                     /locus_tag="PLT63_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99009.1"
                     /translation="MFEYAHLAGEPLFYLFIALSFLLGAGYFWGKRINDRIFRSAFSD
                     LVAVVKPVDQTFTNIGGLIGYHATLRPPKSAPVERVDATITFLPRHAWLWMPVSKLIR
                     KYDRLFITIHLRRAPIAEGHLIEKGYARFRGPKITNVDRLQSEAVRWGNLDFILYFGN
                     ETIRQRLRNFVNKQPDPGLVRHIALVPEQQRSFIFMIPKTGTVAAALAPIYRWIPTIL
                     K"
     gene            complement(2500..3459)
                     /locus_tag="PLT63_09565"
     CDS             complement(2500..3459)
                     /locus_tag="PLT63_09565"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HOD99010.1"
                     /translation="MKVLFFTGKGGVGKSTLAAAAAWQLSRQYRVLAVSLDPAHNLGD
                     VFGVTVGNGGTRVSPRLHLREVDLRKLARDYLERETKVLAESYHYLRVLNLDNYFSIL
                     KYSPGIEEYALLTSIERTLREEDGYDYLVFDTPPTGLTLRFLALPQVTMTWIERLTAI
                     RRQILDKRHTIQKIRGGAKEWEANREIRLEYDESGDDILKRLRLLADNYKVLIRRLQG
                     EECGVVTVFNPDLLSLRESERLITGLRELKLPLVLLIANKVTAENRALADRIAGELQR
                     LASGAAVASVALNPAFTLGGDTPLYEIPEDLTVHFGGGGEDHV"
     gene            complement(3461..3742)
                     /locus_tag="PLT63_09570"
     CDS             complement(3461..3742)
                     /locus_tag="PLT63_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99011.1"
                     /translation="MGGSFLSYCRRLVADMGTAQREGLVALTCLEAQELENIFALLLL
                     GSFVGLPAPPGFLAVELLPYMEREIQVLHRRAEDAGDMLAEIAGTLGID"
     gene            complement(4057..>5457)
                     /locus_tag="PLT63_09575"
     CDS             complement(4057..>5457)
                     /locus_tag="PLT63_09575"
                     /inference="COORDINATES: protein motif:HMM:NF014598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HOD99012.1"
                     /translation="LVVAAFATVISSMFVKTPEIAGASIFVTIVAIILGYLLYQVKMN
                     LPAATVIGIALMAVAIWGGTLMPIALSFEAWLVVLFFYIIIAAAIPVNILLQPRDYLN
                     SWLLYFGIFVGFAAAFFTFRGVEIPMMTEFSPILVGGKPTPFWPAIPLIIACGSLSGF
                     HSLVASGTSSKQITKESDGLFIGYGSMFTEGFLSTIVICAIAGFGFTALRNGHEAAMA
                     AQAAAAAKAAAAGAVAAAGGAAAAAKATVAPFALTLDNWGQLFTKTSEALKLSQANLV
                     VQTYSDMVAASFLSFIPVKIVKIIAGLWISAFALTTLDTTNRIARYCLVEMLAPVKDA
                     ASGIYNVLTNRWIASLVPALIGMYLAWSKQFTIVWPSFGAANQLIASIALMTGAAFVA
                     KKLKSKFAIMAVVPAYFLWVTVTAAIIWFMIVVMPANIAKAPGQGWTVQIIMGIMLVL
                     NFLFIFDFIKMRKQGD"
BASE COUNT         1134 a         1528 c         1698 g         1097 t
ORIGIN      
        1 tgcaggccga ggtggcccag cgcccggtcc tgatcatcct cctcgatgcc cagggcacga
       61 tgacccgcta tggggacgcc aatcgcatca aacagtggat ggagcgctcg ccgccggcgc
      121 cgcggatggt ccaagagggc tgaacgccgg agtgaagccg gtgaaaaaca acgcccatga
      181 cggcttgtca tgtacgatga tttagggaat gtaaggcggg aaagaggaga gggaaagtcg
      241 atgcctccgg aaatgatcgc caagatcgac accgtcctgg aacgggtcaa ggatccggaa
      301 agcggcctgc ccgtggggca attgggtttt atcaagcgct tccgctataa cgaaaaggag
      361 gggaagctgt acgtctttgc cgatacgtac cgtcacctgc ccaaatgtgt tacctgcgcc
      421 gccattgccg gcgcggtgat gtccggtatc atccgggaca ttaccactga acttcagagg
      481 gaatttcccg atctggccat tgagtttgcc ccggcctgaa taaaaatttc tatcatgtag
      541 cgcctgtctc ctgtcttccg ggcaaaccag aaattgttac aaaaaaaacc gccatctgac
      601 acctgtgtca gatggcggcc cgaccatcaa agtcctccag aaatgagttt ctgaacccta
      661 tctcaatatt taattttggc cgaaaggcta taaaattctc tccatgacgt caagagttta
      721 tttgatgtgg ctttgagacg atgcagacta attgtccttg cttcccatat gttttgatca
      781 tcctgccttg atggccaatc tgcaccgcaa ttaaccgggt gatcgtcgcg acgatcaccc
      841 ggaaactgac gggggaaccc ggccgtaatg ggcaaaatta tccggagcgt ttctctgaag
      901 gatacctaga aaaatcttcc gaagcggagt gctgcgtaca tggggtcgtt gtcggcgcgg
      961 aggccggcga ccataacgag gtgcgatttt tcctctttga cgattttttc gaagaatttt
     1021 ttctgatctt tgtccttggc gagtttggcc agctccgtgt agaggaggat ggcgtttttc
     1081 tcggcggcca tggcgatggc ggcggcctgt gctaccgtat ccaccttttc caggcgtttg
     1141 ttgacggtat cgcccttggg gaagaggcct tcctggacga tggcttcgag gtaggtgtcg
     1201 atatccgggt ttttgaggca ctggtcgatg cccttgccct ccgccttggc cttgaactcc
     1261 aggaagatat ccatatgctt ggcctcctcc ttggcgaggg agacgaagag ttttttgaga
     1321 tcttcattgc cggagaattt cttggcggcc ttcatataga agttcatgcc gtccttttcc
     1381 gccctgatgg cgaagttaaa cacacccaaa tcctgttttt gcgccatgat cttacctcct
     1441 tgagtttgat ggttgataaa attatcacgc ccgcacctca tcgcttccgg gctcgggacc
     1501 ggaaggaagc gattgatttt aataaacaga cttgcggccc tcggcgaggc ccttctttgt
     1561 ctccggtttt cccttgcact ccaggggcat gggtatatcc tcgccgggtt cgcaggggag
     1621 gttgctcatg ctcttggcgg cgcagcggcc gattttgaag ccgacatcat aacaggactg
     1681 cggttttgcc gccgccggag gcgcctcctg cgtggctgcc ccttgctgtt gggtgggtga
     1741 gggcggcggc tgttttccac cctcgccgca tccggcaagc atgattacgc cgagcataag
     1801 caccaggctg aagataataa aaacaggtaa ttttcgcatg gttcctccta tttcagaatt
     1861 gttggtatcc agcggtaaat gggggccagg gccgcggcca cggtccccgt tttagggatc
     1921 ataaagatga aactccgctg ctgctccggg accagggcaa tgtggcgcac caggccggga
     1981 tcgggctgtt tgttcacgaa attccggagt ctctgccgga tcgtttcgtt gccgaagtag
     2041 aggatgaaat ccaaattgcc ccagcgcact gcttcggact gcaggcggtc gacattggtg
     2101 atttttggtc cccggaagcg ggcataaccc ttttcgatga gatgcccctc ggcgatgggc
     2161 gcccggcgaa ggtggatggt gatgaagagg cggtcgtatt tgcggatgag cttggagacg
     2221 ggcatccaca gccaggcgtg gcggggcagg aaggtgatgg tggcgtccac ccgttccacc
     2281 ggggccgact tgggcggccg gagggtcgca tgatagccga tgagaccgcc gatgttggtg
     2341 aaggtctgat ccaccggctt gactactgcc accagatccg aaaaggcgct ccggaagatc
     2401 cggtcgttga tccgcttccc ccagaaataa cccgccccca ggagaaagga aagggcgatg
     2461 aagagataga agaggggctc cccggccaga tgggcgtatt caaacatggt cctctcctcc
     2521 cccgccaaag tggacggtca ggtcttcggg tatttcgtac agcggcgtat caccccccag
     2581 ggtgaacgcc gggttcaggg caacgctcgc caccgccgca ccgctggcca ggcgttgcaa
     2641 ctccccggcg atgcggtccg caagggcccg gttttccgcc gtgaccttat tggcgatcag
     2701 gagtacgagg ggcagcttca gctcccggag cccggtaatg agccgctccg attcccgcag
     2761 cgagaggagg tcgggattga agaccgtcac cacgccgcac tcctccccct ggagtctccg
     2821 gatcaggacc ttgtagttgt ccgccagcag cctgaggcgc ttgaggatgt cgtcgccgct
     2881 ctcgtcgtat tccaggcgga tctcccggtt ggcctcccac tccttggcgc cgccgcggat
     2941 cttctgaatg gtatggcgct tgtcgaggat ctgccggcgg atcgccgtga gccgctctat
     3001 ccacgtcatc gtgacctggg gcagggcgag aaaccgcagc gtcaatcccg tcggcggtgt
     3061 gtcgaagacg agatagtcgt agccatcctc ttcccggagg gtccgctcga tactcgtcag
     3121 cagggcgtac tcctcgatgc cgggggagta cttcaggatg gagaagtagt tgtccaggtt
     3181 aaggacccgg agataatggt aggactcggc caggaccttt gtctcccgtt cgaggtaatc
     3241 ccgggccagc ttgcggagat ccacctcccg gagatgcagc ctcggggaaa cccgcgtccc
     3301 gccgttgccc accgtgacgc cgaagacgtc gccaagattg tgggccgggt ccaaagatac
     3361 cgcgaggacc cggtactgcc gggaaagctg ccaggcggcg gccgctgcca gggtggattt
     3421 ccccacccca cccttgccgg taaaaaaaag gaccttcatc tcagtcgatc cccagggtgc
     3481 cggcaatctc cgccagcatg tcgccggcat cctccgcccg gcggtggagg acctggatct
     3541 cccgctccat ataaggcaaa agctccacgg ccagaaaacc gggcggcgcc ggcaaaccca
     3601 caaaagaacc caacagcagc aaggcgaaga tgttctccag ctcctgcgcc tccagacagg
     3661 tcagcgccac cagcccctcc cgctgcgccg tacccatgtc cgccaccaac cgccggcaat
     3721 aactcaaaaa cgaaccaccc ataaaatcct catgcatgct cgccaaccgt gaggagccgc
     3781 ggcgataaaa actcttaccg attcgattat cacacaatcc acagagaaac tctgcctcgg
     3841 ggatgaggcc tgccggctca cggtgatgga gcgtgtcctg ctatcgatat tggcaaccgg
     3901 gatatgcatc ttaaagggaa caagttctgg tcctgccccc ttgctttaaa gatcatttat
     3961 aagatgaaga aaaggtttat gtgccttacc tgttctttca aatgatgcgt atcccggtcg
     4021 ctgcgggcgg ccgggatacg caggtttatg acagcatcaa tctccctgct ttctcatctt
     4081 gatgaagtcg aagatgaaga ggaagttgag gaccagcatg atgcccatga tgatctggac
     4141 ggtccagccc tggccggggg ccttggcgat gttggccggc atgacgacga tcatgaacca
     4201 gatgatggcg gcggttacgg tgacccagag gaaataggcg gggaccaccg ccatgatggc
     4261 gaatttggat ttcagctttt tggccacgaa ggccgccccg gtcatgaggg cgatagaggc
     4321 gatgagctgg ttggcggcgc cgaaggacgg ccagacgatg gtgaactgct tgctccaggc
     4381 caggtacatg ccgatgaggg ccgggaccag ggaggcgatc cagcggttcg tcaggacgtt
     4441 gtagataccc gaggcggcgt ctttcaccgg ggccagcatc tccacgaggc agtaccgggc
     4501 gatgcggttt gtcgtatcca gggtggtgag ggcgaaggcg gagatccaca gaccggcgat
     4561 gatcttgacg atcttcacgg ggatgaagga gaggaagctc gccgccacca tgtcggagta
     4621 ggtctggacg acgaggttgg cctgggagag cttcagggcc tccgaggtct tggtgaagag
     4681 ctgcccccag ttgtccaggg tcagggcgaa gggggcgacg gtagccttgg ccgcggccgc
     4741 ggcgccgccg gctgccgcca ccgcccccgc ggctgccgct ttcgcggcgg cggcggcctg
     4801 ggcggccata gccgcttcat gaccgttcct gagggccgtg aagccgaagc cggcgatggc
     4861 gcaaatgacg atggtggaga ggaaaccctc ggtgaacatg gagccgtaac cgatgaagag
     4921 gccgtcagac tccttggtga tctgtttgga cgaggttccc gaggcgacga gggagtggaa
     4981 gcccgacagg gaaccgcagg cgatgatcag ggggatggcg ggccagaacg gcgtcggctt
     5041 gccacccacc aggatcggcg agaactcggt catcatgggg atctccacgc cccggaaggt
     5101 gaagaaggcg gcggcgaagc ccacgaagat cccgaagtag agcagccagg aattcaggta
     5161 gtcccggggc tgtagcagaa tgttcaccgg gatggccgcg gcgataatga tatagaagaa
     5221 gaggaccacc agccaggcct cgaagctcag ggcgatgggc atgagtgttc ctccccagat
     5281 ggccacggcc attaaggcga tcccgatgac ggtggcggcg gggaggttca tcttcacctg
     5341 gtagaggaga tacccgagaa taatagcgac gatggtgacg aagatggagg ccccggcgat
     5401 ctccggcgtc ttgacgaaca tggaggagat gaccgtggcg aaggccgcga cgacgag
//
LOCUS       DAOSMF010000128         5395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_16623_length_5395_cov_25.408801, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000128 DAOSMF010000000
VERSION     DAOSMF010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5395
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16623_length_5395_cov_25.408801"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2058
                     /locus_tag="PLT63_09580"
     CDS             <1..2058
                     /locus_tag="PLT63_09580"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HOD99013.1"
                     /translation="KFKAEKITNAIAKAGMATGEFDDKIARKMTIKVLNLANRLFDSM
                     IMTVEEVQDIVEEVLLDSPYRRTAKAYIIYRDQHARLREVANKMEIDLVDQYLRKIDW
                     KVHENSNMDYSLQGLNNYISSEVSKVYWLNEIYSPEIRKAHSEGDFHIHDLSLLSVYC
                     VGWDLYDLLLEGFKGVSGKVESKPARHLRSILGQVVNFFYTLQGEAAGAQAFSNFDTL
                     LAPFIRSDGLSYQEIKQVLQEFIFNINVPTRVGFQTPFTNVTLDVTVPRHFADQHVII
                     GGVPRRETYGECQKEMNLFNKAFLEVMAAGDAKGRVFTFPIPTYNVTRDFKWEDPNLD
                     GLWEMTAKYGVPYFSNFINSDMSPEDARSMCCRLRIDNRDLERRGGGLFGSHPLTGSI
                     GVITINMPRIGHLAASESEFFQRLERLMIIARESLETKRKVLEKFTDGNLYPYTKYYL
                     RKMKERFGEYWKNHFSTIGLVGMNEACLNLLGRDIGTPEGQDFTKRVLDFMREKLIAF
                     QKATKNNYNLESTPAEGTSYRLARLDKQKYPEARCANEEAFRSRGAEPFYTNSTQLPV
                     NFTDDIFTALDLQDGIQTKYTGGTVFHAFAGERIDDPAAVRCLVRKVCSQYRLPYLTF
                     TPTFSVCPSHGYLKGEQTVCSECGEPCEVYSRVVGYLRPVKQWNRGKQEEFKLRRTFR
                     AAG"
     gene            2055..2744
                     /locus_tag="PLT63_09585"
     CDS             2055..2744
                     /locus_tag="PLT63_09585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HOD99014.1"
                     /translation="MKIGGWQRFSLIDYPGRIGAVVFTQGCNFRCPYCHNPELVDPRR
                     FGKTIPPGDIISFLAGRRGKLDAVTITGGEPTRQGDLADFLTRVKDLGFLVKLDTNGS
                     APFTLAMLLEKGLVDYIAMDIKGPLEKYAAIAGTEVNGRDIVASVSLISGAGIPHEFR
                     TTLVAPFITPEDVVATAGLIPAESRYVLQRFVPAKLLASGLGEINCFTDIVIAALKVR
                     LEKHLSCVAVR"
     gene            2757..4343
                     /locus_tag="PLT63_09590"
     CDS             2757..4343
                     /locus_tag="PLT63_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOD99015.1"
                     /translation="MKRRSTTNSLSVVERLSKIGVALSAQRDVNRLLELIIDEACGLT
                     AADGGTLYTLSCEGNFLEFAIIYNQTLGIRKGGAGEPIDWPPVRLKRGVKRRNMANVS
                     AYAALTGKVVNIPDVYNAAGFNFQGTKDFDARSGYRSQSMLVVPMRNHEGDIIGVLQL
                     LNAKDEKTGQVMPFSRESVMITESFASQAAVAISQNRLIKSLEDLLDAFVKTIAQAID
                     EKSPYTGGHIRRVADLTMSIADKINTVQTGRFAGVRFSDHEMQELRMAAWLHDVGKIT
                     TPERIVDKATKLMAACDRLELIKTRGEVMIRDHELAQGRHNDPYPDGERAKITKDDDF
                     ARQIQADMDYLASLNMGASPVRPDDIARLKAIASRKWRQDGQDKPLLTDDEYQNLAVF
                     RGTLTDAEREIINNHALITKRLLENLPFPKRLKNIPVFAGSHHEKLDGSGYPQGMAGE
                     EIPLQARIIALADVFEALTAGDRPYKRANTLSEAVAILRDMAKMRHIDADLFSLFIEE
                     KIYLDYAVREMQPKQIDMED"
     gene            complement(4481..>5395)
                     /locus_tag="PLT63_09595"
     CDS             complement(4481..>5395)
                     /locus_tag="PLT63_09595"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HOD99016.1"
                     /translation="ADVIIENARPGTMEKIGLGYEEMRCLNSRLIYCSLTGFGQTGPY
                     RHRPAHDINILSISGIHDLLGESGRSPIVPAVMISGAGGGGLNAALGILSSLLRRERT
                     GAGQYLDVSILDGLSPFMALMMSEYLAQRIDPVRGESRLGGGYACYNVYETRDGKFIS
                     IGCLEEKFWQELCSCLGREEFRPNLNAPRARQAEMIDEFRRIFRQKDRRDWLEILDRY
                     DICFSPVNSLAEACEDPQIQARQLWFYTPHPSDGSVPQQAFPIRFSADQPGLRNPPPL
                     LGQHTGEILRELGYSEDDLAELKSHGII"
BASE COUNT         1250 a         1484 c         1512 g         1149 t
ORIGIN      
        1 aaattcaagg ccgagaagat caccaatgcc atcgccaagg ccggaatggc cacgggagaa
       61 ttcgacgaca aaattgcgcg caagatgacc atcaaggtcc tgaacctggc caatagactt
      121 ttcgacagca tgatcatgac ggtggaggag gttcaggaca tcgtcgaaga ggtgcttctg
      181 gattcgccct accggcggac cgccaaggct tatatcatct accgggatca gcatgcccgc
      241 ctgcgggagg tcgcgaataa aatggagatc gacctcgtgg atcaatacct ccggaagatc
      301 gactggaagg tgcatgaaaa cagtaacatg gattattcgc tccagggatt gaacaactat
      361 atatcttccg aggtcagcaa ggtttactgg ctcaacgaga tctattcccc ggagatccgg
      421 aaggcccata gcgaggggga tttccatatc catgacctga gtctgttgtc tgtttactgc
      481 gtaggctggg acctttacga tctgctccta gaaggattca agggcgtctc tggcaaggtg
      541 gagagcaaac cggcgcgaca tctccgcagt attctggggc aggtggtcaa tttcttctat
      601 accctacagg gggaggcggc gggggcccag gccttttcca atttcgatac cctcctggcg
      661 cccttcatcc gctccgatgg cctcagttac caggagatca agcaggtcct ccaggaattc
      721 atcttcaata tcaacgttcc cacccgggta gggtttcaga ccccctttac caatgttacc
      781 ctcgatgtca ccgtaccccg gcactttgca gatcaacacg tgatcatcgg cggcgttccg
      841 cggcgggaaa catacggtga atgccaaaag gagatgaatc tgttcaacaa ggcgtttctt
      901 gaggtcatgg ccgccggcga tgccaagggt cgggtcttta cctttcctat cccgacctac
      961 aatgtcaccc gggacttcaa atgggaagat cctaacctcg acggtctctg ggaaatgacg
     1021 gccaaatacg gggtgcccta tttctccaat ttcatcaact ccgatatgag ccccgaggac
     1081 gcccggagca tgtgctgccg gttgcgcatc gacaaccggg acctggagag acggggggga
     1141 ggtctgttcg ggtcccatcc cctgaccggt tccatcggcg tcatcaccat caacatgccg
     1201 cggatcggtc acctcgccgc ttcggaaagc gaatttttcc agcgcctgga aaggttgatg
     1261 atcattgccc gggaaagctt ggagacgaag cgcaaggtcc tggaaaaatt caccgacggc
     1321 aatctctatc cctacaccaa atattatctg cggaagatga aggagcgttt cggcgaatac
     1381 tggaagaatc acttttccac catcggattg gtggggatga acgaggcctg cctgaatctg
     1441 ctggggcggg acatcgggac gccggaagga caggatttta ccaaacgggt gctcgatttc
     1501 atgagagaga agctcattgc ctttcagaag gctacgaaga acaactacaa cctggaatcg
     1561 acaccggcgg aggggacgtc ctaccgcctg gcccggttgg acaagcagaa ataccccgag
     1621 gcccggtgtg ccaacgaaga ggccttccga agccgggggg cagagccttt ttacaccaat
     1681 tccacgcaat tgccggtcaa cttcacggat gacatcttca cggccctgga tcttcaggac
     1741 ggtatccaga cgaaatatac cggcgggaca gtatttcatg ccttcgccgg ggagcggatc
     1801 gacgatcccg ctgccgtgcg ctgcctggta cgtaaggtct gcagccagta ccgactcccc
     1861 tacctcacct ttacccctac cttcagcgtt tgtccttccc atggctatct caagggggaa
     1921 cagaccgttt gttcagaatg tggagaaccc tgcgaggttt attcacgggt ggtgggctac
     1981 ctccggccgg tgaagcagtg gaaccgggga aagcaggagg aattcaagct ccgcaggaca
     2041 ttccgggccg cgggatgaag atcgggggct ggcagaggtt ctccctcatc gattatcccg
     2101 gccgcatcgg cgccgtggtc ttcacccagg gatgcaactt ccgctgcccc tattgtcaca
     2161 atcccgaact cgtcgatccc cgccgcttcg ggaagacgat tccccccggg gatattatct
     2221 cattcctcgc cggacgccgg gggaaattgg atgctgtcac cataaccggc ggtgagccaa
     2281 cccggcaggg agaccttgcc gattttttga cccgcgtcaa agatttgggt tttcttgtga
     2341 agcttgacac caacggctcc gctccattca ccttggccat gcttctggaa aaaggattgg
     2401 tggattatat tgccatggat ataaaagggc cgctggagaa atatgccgcc attgccggca
     2461 ccgaggtaaa cggcagggat attgtggcaa gtgtctcatt gatctccggc gccggcattc
     2521 cccatgagtt ccggaccacc ctggtggctc catttattac gccggaggat gttgtcgcca
     2581 cggcaggact cattcccgca gaatcaagat atgtactgca gcgatttgtg ccggcgaaac
     2641 tcctcgcttc cggactggga gagataaatt gctttacgga cattgtgatt gccgcgttga
     2701 aagttcgact tgaaaaacat ctttcctgtg tggcagtccg atgatatgat gaggatatga
     2761 agagaagatc aacgaccaat tccttgagcg ttgtcgagcg tctgtccaaa atcggcgtgg
     2821 ccctgtcggc gcaacgggac gtcaaccgcc tgctggagct gataatcgac gaggcctgcg
     2881 gtttgacggc cgcggatggg gggaccttgt ataccctgtc ttgtgaaggc aactttctcg
     2941 aatttgccat tatctataat caaacgctgg gcattcgcaa aggcggcgcc ggggagccga
     3001 tcgactggcc gcccgtccgg ctcaagcggg gtgtgaagag acggaacatg gccaatgttt
     3061 ccgcctatgc cgccctcacc ggcaaggtcg tcaatattcc tgatgtctat aatgccgcag
     3121 gtttcaattt ccagggaacc aaagatttcg atgcccgttc cggttatcgt tcccagtcga
     3181 tgctcgttgt ccccatgaga aatcacgaag gggatatcat cggtgtcctg caattgttga
     3241 atgccaaaga tgaaaagacg ggacaggtga tgcccttttc ccgggagagc gtaatgatca
     3301 ccgaatcatt tgcctcgcaa gccgccgtgg ccatttcgca aaaccggctg attaaaagcc
     3361 tcgaagatct tctggatgcc tttgtaaaga ccatcgccca ggctatcgac gaaaagtctc
     3421 cctataccgg cggacacatc agacgggtgg ccgacctcac catgtccatt gcggataaaa
     3481 tcaacacggt gcaaacaggt cggttcgccg gggttcgttt ttccgaccat gagatgcagg
     3541 aacttcggat ggccgcatgg cttcacgatg ttgggaaaat aactactccc gaacgtattg
     3601 ttgacaaggc aaccaagctc atggctgcct gcgaccgcct ggagctgatc aaaacccggg
     3661 gggaggtgat gatcagggac catgaactgg cccaggggcg acataatgat ccgtatccgg
     3721 acggtgagcg ggcaaagata acgaaagatg atgattttgc ccgacaaata caggcggata
     3781 tggattattt ggcctctctg aacatggggg cgtcgccagt ccgtccggat gacatcgccc
     3841 gtctcaaggc gatcgccagc cggaaatggc ggcaggacgg ccaggataaa cctcttctta
     3901 ccgatgatga gtaccaaaac ctggccgtat tccgcggcac cctgacggat gccgaacggg
     3961 aaatcatcaa caatcacgcc cttattacca agagactcct ggaaaatcta ccgtttccca
     4021 agcggctgaa gaacatcccc gtctttgccg gcagccacca tgaaaagctc gacggttccg
     4081 gctatcccca gggcatggcg ggggaggaaa taccactcca ggcgcgtatc attgccttgg
     4141 ccgatgtctt tgaggcccta acggcgggcg accggcccta caaacgggcc aacacccttt
     4201 ccgaagccgt cgccattctc cgagacatgg cgaagatgcg gcacatcgat gcggatctgt
     4261 tctccttgtt cattgaggag aagatctatc tcgattacgc cgtgagggaa atgcagccaa
     4321 agcaaatcga catggaggat taggaggaac aatcagttcg gctgatttaa gaacaataat
     4381 cagcttcgct gatttccgtc gatcggaaag gataatcagg tggaagcggt atcagtgcct
     4441 gcctaagcaa tcgtcttatc gaggggcaaa ggctttgccc tcaaatgatc ccgtgtgact
     4501 tcagttcggc gagatcgtct tccgaatatc ccagctctct caaaatttcc ccggtatgct
     4561 gaccaagaag cggcggcggg ttccgcagcc cgggttgatc ggcggaaaac ctgatgggaa
     4621 atgcctgctg gggaacggaa ccgtccgaag gatggggggt gtaaaaccag agttgccgcg
     4681 cctggatctg gggatcctca caggcctccg caagggagtt taccggggaa aaacagatgt
     4741 cgtagcggtc caggatttct aaccagtccc ggcggtcctt ctgccggaag atccgccgga
     4801 attcatcgat catctccgcc tggcgggccc ggggcgcgtt caggttcgga cggaattcct
     4861 cccgtcccaa acaggaacag agttcctgcc agaacttctc ctccaggcaa ccgatcgaga
     4921 tgaatttacc gtctctcgtt tcatagacat tgtagcaggc gtaaccccct cccaaacgtg
     4981 attccccccg gaccggatct atccgttggg cgagatattc cgacatcatc aacgccatga
     5041 agggactcag gccgtcgagg atcgagacgt cgaggtactg tccggctccc gtccgttccc
     5101 gacgcaacag tgaggaaagg attcccaggg cagcgttcaa tccccccccg ccggccccgg
     5161 agatcatgac ggcggggact attggggagc ggccactttc tcctagcaga tcgtggattc
     5221 cggaaatgga caggatgttg atatcgtggg cgggacggtg gcgatacgga ccggtttggc
     5281 cgaatcccgt caatgagcaa tagatcagcc gggagtttag gcaacgcatc tcttcatagc
     5341 ccagaccgat tttttccatc gttccgggac gggcgttttc gatgattacg tcggc
//
LOCUS       DAOSMF010000129         5384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_16665_length_5384_cov_19.847251, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000129 DAOSMF010000000
VERSION     DAOSMF010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5384
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16665_length_5384_cov_19.847251"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="PLT63_09600"
     CDS             <1..864
                     /locus_tag="PLT63_09600"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOD99017.1"
                     /translation="GAFQYYLTASVINELLKDPTKLKLGGDKKDLTVMFSDIRGFTTI
                     SEKLTPEELVHLLNEYLTAMTDIVFKYEGLLDKYIGDAIMAVFGAPVDQPDHALRSCR
                     TALEMMATLKGLQQKWAAEGRPFVDIGVGINSGDMVVGNMGSNMRFDYTVMGDNVNLS
                     SRLEGINKEYGTHIVISEFTYEVVKEEVFCRELDAVRVKGKKLPVKIYELICERKDAA
                     EHEEYIGRFHAGLAHYKAARWDEGIAAFKSVLEIRPDDPPAKLYIQRCQDLKENPPEG
                     EWDGVFTMTRK"
     gene            831..2825
                     /gene="alr"
                     /locus_tag="PLT63_09605"
     CDS             831..2825
                     /gene="alr"
                     /locus_tag="PLT63_09605"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOD99018.1"
                     /translation="MGRRVHHDQEMTMSYREVHKYRSWVEVDLDYFARNWKEMKRLVR
                     PGVKILQVVKADAYGHGAIEIAEAALKNGAVYLGVANADEGVQLRVSGLMAPIVILSP
                     SPEGEIGEIIKYNLTPSVSDLHFARTLQDRCAQAHVRLPVHIEVDTGMGRGGTLYYEA
                     QQTIKNILALPDLIVEGLFTHLAASETLQDYNESQAELFHRLLAKLAEEGIRIPIRHM
                     SNSGAILNYPQFNLDMVRPGLMSYGIYPSSETKDKADLLPVMSFKTRVVLLKDFPKGY
                     SIGYNRTYITTRPTRIATIPVGYGDGYGVILSNQGEVLIRGKRAPVVGRVSMDMCTVD
                     VSEIPECQVDDEVVLMGRQGHEAISANEIARRVGSISYEILCALGKRAPRLFLHKGKA
                     NSVLPRLRRIYVPGEERSIDRIDSIIRQCFQTRARSEELGNAIYYEMFETLFGKEDRQ
                     LELREDFRYDITVAESAGENGGPAGRRGDSLDVTTRVAYRKTLKNDTFLIGCAFDEEQ
                     LAAFFEDPRCEYRWLMSKGEHLVMERNFRVAGVAVDGRDVPIVRTEMTSRGYEVWCSS
                     DSLRDKLNTEVEIAIEIFTKKSRANNIFSVYIVYPTRGLDISFHYEQTGFRHVREVSF
                     FAGKQPYPEVIRERGRYIRLRVPNHTWVFPNSGVTFIWDS"
     gene            2843..>3792
                     /locus_tag="PLT63_09610"
     CDS             2843..>3792
                     /locus_tag="PLT63_09610"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99019.1"
                     /translation="MEKLRVGIILGGMSSEREVSLNSGRNVYDNLDPEFFHGVPLFMD
                     AAGGIWEIPWQLVSQNTTVDIGERLTVEARRIPYEDLKGRIDFAFISLHGKYGDDGCI
                     QGLLELLGIPYTGPGVLASALGMDKHIQQKILAAAGIAVPVSVAVHEGEWRRAPEAVT
                     KKLLQNFELPFFIKPVREGSSVGVGKIAAADDIAGTLEEAFRWDRVALAEEFLTGIEF
                     SAIILEQDGEIMPLDLTEIHPQSDFYTYDDKYMPGRCRKFTPPKNIPPEVVARIKEEA
                     RRAFEVLGFRSYGRIDGFYLPDGRILITDPNSSSGMAPSS"
     assembly_gap    3793..3892
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3893..4834
                     /locus_tag="PLT63_09615"
     CDS             <3893..4834
                     /locus_tag="PLT63_09615"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99020.1"
                     /translation="PLAVFMDSQARLWEIPLKLLMRNSTRDIEDDLETEAQRVPYETM
                     KEKADMVCLGLHGKYGEDGCMQGLLELLGIPYTGSGVLGSALGMDKYVCRRILAMNGI
                     DVPATIPVYRRAWDGGGREEILARVEREIGFPCVVKPTREGCSTAVKKVMSGAGVAEA
                     VLSALEWDNVVLVEEFLTGTEVTCGVLDDDPPLALIPSETIPTAEILSLEDKFLYGQG
                     ENKTPARLPTEVLKRVQDTALGAFAALDLKGYARIDMFVRTDGRVAVLEPNTLPGMTP
                     STVLFHQAAAAGITQTGLISRIIDAALRAHSAKRGPL"
     gene            4836..>5384
                     /locus_tag="PLT63_09620"
     CDS             4836..>5384
                     /locus_tag="PLT63_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOD99021.1"
                     /translation="MGRVTKAIDALGRSVLYSVEEMGRIVLLFISVLGWMFRPPLRLR
                     LIFKQMEFVGVRSIFVVVLTGMFTGMVMALQGYHGFRMFSAEGMVGATVALGMTRELG
                     PVLTSLMVTARAGSAMAAEIGTMKVTEQVDALYVMAANPIQYLIVPRVIAGVIMVPFL
                     TIISDFVGIVGGYFVGVGLLNIN"
BASE COUNT         1072 a         1518 c         1639 g         1055 t
ORIGIN      
        1 ggggcctttc agtattatct gacggcctcg gtcatcaacg aactgctcaa ggatcccact
       61 aaactgaaac tggggggcga caagaaggat cttaccgtca tgttttccga tatccgtggc
      121 ttcaccacca tttcggaaaa gttgaccccg gaggaactcg tacacctcct caatgagtat
      181 ttgacggcca tgaccgatat cgtcttcaaa tatgagggac ttctcgacaa atatatcggc
      241 gatgccatca tggccgtctt tggcgctccg gtggaccagc cggatcatgc cctgcggagt
      301 tgccggaccg cactggagat gatggcgacc ctcaaggggc tgcagcaaaa atgggcggcg
      361 gaagggcggc cctttgtgga catcggggtc ggcatcaaca gcggcgacat ggtcgtaggc
      421 aacatgggtt ccaatatgcg cttcgattat accgtcatgg gcgacaacgt gaatctcagc
      481 tcccgcctcg aggggatcaa caaggagtat gggacccata tcgtcatcag tgaatttacc
      541 tatgaagtgg tgaaagagga ggtgttctgt cgggagttgg acgcagtgcg cgtcaagggg
      601 aagaagctgc ccgtgaagat ctacgagttg atctgtgagc ggaaggatgc ggcggaacac
      661 gaggagtaca tcgggcgttt ccatgcggga ctggcgcact acaaggccgc ccgctgggat
      721 gagggcatcg cggctttcaa aagcgttctg gaaattcgtc ccgacgatcc gccggcgaag
      781 ctgtatattc agcgctgtca ggatctgaag gaaaatcctc cggaaggtga atgggacggc
      841 gtgttcacca tgaccaggaa atgaccatgt cctaccggga agtccataag tatcggagct
      901 gggtggaggt cgatctcgac tatttcgccc ggaactggaa ggagatgaaa cgcctggtca
      961 ggccgggcgt caagatcctc caggtggtaa aggctgatgc ttacggtcac ggggccatcg
     1021 agatcgccga ggcggccctg aaaaacggcg ccgtctatct gggggtcgcc aacgccgacg
     1081 aaggggtaca gctccgggtc agcgggctta tggcccccat cgtcatcctc agcccctctc
     1141 cggaagggga aatcggcgag atcatcaagt acaacctcac cccttccgtc tccgatctcc
     1201 atttcgcccg gaccctgcag gatcgttgcg cccaggccca cgtccgtctt cccgtccata
     1261 tcgaggtgga tacggggatg gggcggggcg gaacacttta ttacgaggcc cagcagacca
     1321 tcaagaacat cctggcgctt ccggatctga ttgtggaagg gcttttcacc catttggcag
     1381 ccagtgaaac cctgcaggac tacaatgaat ctcaagcgga gctgttccac cgcctgctgg
     1441 cgaaactggc cgaggagggg atccgcatcc ccatccggca catgtccaac agcggcgcca
     1501 tcctgaacta tccccaattc aacctggaca tggtgcgccc ggggctcatg tcctacggca
     1561 tctatccctc ctcggaaaca aaggacaagg cggacctcct accggtgatg tccttcaaga
     1621 cgcgcgtcgt cctcctgaag gattttccca agggttacag catcggctac aaccgcacct
     1681 atatcacgac gagaccgaca cggattgcca ccattcccgt cggttacggc gacggttacg
     1741 gcgtcatcct ctccaatcag ggggaggtgc tgattcgagg aaaacgggcg cccgtcgtcg
     1801 ggcgtgtttc catggacatg tgcaccgtgg atgtgagcga gatccccgag tgccaggttg
     1861 acgacgaggt ggtgctcatg gggcgccagg ggcatgaagc gatctccgcc aacgagatcg
     1921 ccagaagggt gggaagcatc agctacgaaa tcctctgtgc cctgggcaaa cgggctcccc
     1981 ggctctttct ccacaaggga aaggcgaaca gcgtcctgcc ccggctccgg cggatctatg
     2041 tccccggaga ggagcgttcc atcgaccgga tcgacagcat catccgccaa tgttttcaga
     2101 cccgggcccg gagtgaggaa ttggggaacg ccatctacta tgagatgttc gagaccctct
     2161 ttggcaagga ggaccgccag ttggagttgc gggaagactt ccggtatgat atcaccgtgg
     2221 ccgaatccgc gggggagaac ggcggacccg ccggccgtcg cggcgactcc ctggacgtaa
     2281 ccacccgggt ggcataccga aaaactttga aaaacgacac ctttcttatc ggctgcgcct
     2341 tcgacgaaga gcagttggcg gcgtttttcg aggatccccg ctgcgaatac cgctggctga
     2401 tgagcaaggg ggaacatctg gtcatggagc gtaattttcg ggttgccggg gttgccgtgg
     2461 acggccggga cgtgcccatc gtccgcaccg agatgacctc tcggggctac gaggtctggt
     2521 gcagcagcga cagcctgcgg gacaagctca acaccgaggt ggaaatcgcc atcgagatct
     2581 tcacgaaaaa atcgcgggcg aacaatatct tctccgtcta tatcgtgtat ccgactcggg
     2641 gtctcgatat ctccttccat tacgaacaaa cgggattccg gcacgtccgg gaggtaagtt
     2701 tcttcgccgg caaacagccg tatccggagg tgatcaggga aaggggccga tatatacggc
     2761 tgcgggttcc caatcatacc tgggtcttcc cgaacagcgg tgtcaccttt atctgggatt
     2821 cataaggagg ttctggaggc ggatggaaaa actgagagtg ggaatcattc tgggtggtat
     2881 gtcgtcggag cgggaggtgt ccctcaacag cggccggaat gtttatgaca atctcgatcc
     2941 ggagttcttt cacggagtcc ccctgttcat ggatgccgcc ggcggcatat gggagatccc
     3001 ctggcagctc gtctcccaga acacgacggt ggatatcggc gagcgcctga ccgtcgaggc
     3061 ccggcggatt ccctatgagg atctcaaggg gcggatcgat ttcgcgttca tctcccttca
     3121 tggcaagtac ggcgacgacg gctgcatcca gggtctgctc gaactcctcg gaattcccta
     3181 tacggggccc ggggttctgg cctctgccct gggaatggac aagcatatcc aacagaagat
     3241 cctggcggcg gcggggattg ccgtgccggt aagcgtggct gtccatgagg gggaatggcg
     3301 gcgggcgccg gaagcggtga cgaaaaaact gctccaaaac tttgagctgc ccttcttcat
     3361 caagccggtc cgggagggat cgagcgtcgg cgtgggcaag atcgcggcgg cggacgatat
     3421 cgccgggacc ctcgaggagg ctttccgctg ggaccgggtc gccctggcgg aggagttcct
     3481 caccggcatc gagttttccg ccatcatcct cgaacaggat ggggaaatca tgcccctgga
     3541 cctgacggag atccatcccc agagcgactt ctacacctat gacgacaagt acatgcccgg
     3601 ccgttgccgg aaattcaccc cgccgaagaa tatccccccg gaggtggtgg cccggatcaa
     3661 ggaagaggcc cgccgggcct ttgaagtgct gggatttcgt tcctacggcc gcatcgatgg
     3721 cttttatctc cccgacggcc ggattctcat caccgatccg aattcgtctt ccgggatggc
     3781 cccctcctcg ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncccctggc
     3901 cgtattcatg gactcccagg cgcggctctg ggagatcccc ctgaagctcc ttatgaggaa
     3961 cagcacccgg gatatcgagg atgatctgga aaccgaggcg caacgcgttc cctatgaaac
     4021 catgaaagaa aaggccgata tggtctgcct gggactccac ggcaaatacg gtgaggacgg
     4081 ctgtatgcag ggtctcctgg aattgctggg aatcccctat acgggatcgg gagtcctcgg
     4141 ttccgctctc ggcatggaca agtacgtctg ccgccggatc ctggccatga acggcatcga
     4201 tgttccggct accatccccg tttatcgccg ggcgtgggac ggcggcggcc gggaggagat
     4261 cctggcccgg gtggagcggg agatcggctt cccctgcgtg gtcaaaccca cccgggaggg
     4321 atgcagcacc gctgtcaaga aggttatgtc gggagcgggg gtggctgagg cagtcttatc
     4381 cgccctggaa tgggacaatg tcgtcctggt ggaggaattt ctcacgggaa cggaggtcac
     4441 ctgcggcgtt ctggatgacg atcccccgct ggccctcatc ccttcggaga caatccccac
     4501 ggccgaaatc ctctccctgg aggacaagtt cctctacgga cagggagaga acaaaacccc
     4561 ggcccgcctg cccacggagg tcttgaaaag ggttcaggat acggccctgg gtgcctttgc
     4621 cgccctggac ctcaaaggct atgcccgcat cgacatgttc gtgaggaccg atggccgggt
     4681 ggccgtcctc gagcccaaca ccctgccggg gatgaccccg tccacggtgt tgtttcatca
     4741 ggcggcggcg gcaggcatta cccagaccgg tctgatcagc cggattatcg acgccgccct
     4801 gcgggctcac agcgccaaaa ggggcccgct gtagcatggg cagagtcacc aaggcgatag
     4861 atgccctggg gaggtccgtc ctctattccg tagaagaaat ggggcggatc gtcctcctgt
     4921 tcatttccgt cctggggtgg atgttccggc cgcccctgcg cctccgcctg atcttcaaac
     4981 agatggaatt cgtgggcgtc cgctccatct ttgtggtggt gctcaccggc atgtttaccg
     5041 gtatggtcat ggccctccag gggtatcacg gctttcggat gttcagcgcc gagggcatgg
     5101 tgggggcgac cgtggccctg gggatgacca gggagctggg gcccgttctt acctccctca
     5161 tggtgacggc tcgggccggt tcggccatgg cggcggagat cggcaccatg aaggtgaccg
     5221 aacaggtgga cgcactctat gttatggctg ccaatcccat ccagtatctg attgtccccc
     5281 gtgtcattgc cggcgtcatt atggtgccct ttctgaccat catcagcgat tttgtaggca
     5341 tcgtgggcgg ttattttgtc ggcgtggggc tcctgaacat caat
//
LOCUS       DAOSMF010000130         5384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_16666_length_5384_cov_17.816476, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000130 DAOSMF010000000
VERSION     DAOSMF010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5384
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16666_length_5384_cov_17.816476"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="PLT63_09625"
     CDS             complement(<1..184)
                     /locus_tag="PLT63_09625"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOD99022.1"
                     /translation="MDFELTEELKMLKDMAYKFAVTEFTPISKECDEHEKYTPEVRKK
                     AAENGLVAAWIPEEYGG"
     gene            complement(337..1185)
                     /locus_tag="PLT63_09630"
     CDS             complement(337..1185)
                     /locus_tag="PLT63_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HOD99023.1"
                     /translation="MEIKKICVLGAGLMGNGITQVCAQAGYEVAMRDIEQRFIDGGMK
                     TITRNLSKDVEKGKRTKEDMDAILGRIKPTLDLKEAAAGADLVVEVVIEVLEVKKQVY
                     KELMAVVSKDCYFFTNTSGLSITEMAAITDRPDKFIGTHFFNPVPVMKLLEVIRGHET
                     SDETVEVARAWGKKIGKEVVVVKEAPAFAVNRILCTMLNEAFFVLDEGIASAADIDKA
                     MVLGCNHPIGPLALSDLVGNETLLRVIEGLHRELGDKYRPAPLLRKLVRAGRFGRKTG
                     KGVYDY"
     gene            complement(1257..2624)
                     /locus_tag="PLT63_09635"
     CDS             complement(1257..2624)
                     /locus_tag="PLT63_09635"
                     /inference="COORDINATES: protein motif:HMM:NF014594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HOD99024.1"
                     /translation="MSKYGNKLATPEQVASLVRDGMWVDYGTVQAPRAFDRALAQRVG
                     EVKNVKIRALGHLTPLEVMEKDPEAKSFWIGNWHILGWTRKYIAENRGTHIPYNFGEA
                     ARYYRDGLDVDIAVQIVTPMDKHGYFNFGPLCNFKKAVFERAKIIVVEVVEDMPWCYG
                     GYDECIHISEVDYVIENNTDKLITIPTPQPTAVEEKIAGYIAELIPKDGPTLQVGIGG
                     LPDTVLRLLADSGARDIGIHTEMVTEGIADLHDAGLITGKKKTLLPGKIVWAFAIGSR
                     RLYDWIDHNPALAMYPVEFTNDPNIIAMNKDLISINACLGVDLQGQVSSESLGFAQYS
                     GTGGQLAFVRGAYYRATKGQPPLGYPENKSVLAFQSTYTDKEGKLKSKITPTLPPGEI
                     VTVPRVDVSYLVTEHGVAYLKGLSIPERVLAITKLAHPDFRDELLEEAKKMHWLNKQW
                     FLGAI"
     gene            complement(3331..3420)
                     /locus_tag="PLT63_09640"
     CDS             complement(3331..3420)
                     /locus_tag="PLT63_09640"
                     /inference="COORDINATES: protein motif:HMM:NF019820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AURKAIP1/COX24 domain-containing protein"
                     /protein_id="HOD99025.1"
                     /translation="MGSVVKKRKKKISKHKYKKRLKANRHKKK"
     gene            complement(3449..3525)
                     /locus_tag="PLT63_09645"
     tRNA            complement(3449..3525)
                     /locus_tag="PLT63_09645"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3489..3491),aa:Lys,seq:ttt)
     gene            complement(3610..4914)
                     /locus_tag="PLT63_09650"
     CDS             complement(3610..4914)
                     /locus_tag="PLT63_09650"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041584774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOD99026.1"
                     /translation="MANVIVVGTQWGDEGKGKIVDLYAENADVIARFQGGNNAGHTLV
                     VKGVQTILHLVPSGILHDQKLCIIGNGVVVDPRVLITEIDALEERGLFPPQTQLYVSE
                     KAHLIMPYHKRLDLAREGKRGGGKIGTTGRGIGPAYEDKVARTGIRVGELTGDGDAFR
                     EKLRRNLEEKNFLLTKYFGEAPVDPEAVYEEHRAYGERLREYVADTSVILYRELRRGK
                     RILFEGAQGCHLDIDHGTYPFVTSSNTVSANAFSGTGVGPSAAFAVVGICKAYTTRVG
                     GGPFATELDDEIGRRLQEVGKEFGATTGRRRRCGWLDMVLVRHSVRVGGVTGIALTKL
                     DVLTGIDKIKICIGYRTAEGEQINLAVPADLRTLAGCEPVYEELEGWTEDISQARHLE
                     ELPKNVRKYLERIETLADVPLVLVSVGAARNETIILKNPFSS"
     gene            complement(5179..>5384)
                     /locus_tag="PLT63_09655"
     CDS             complement(5179..>5384)
                     /locus_tag="PLT63_09655"
                     /inference="COORDINATES: protein motif:HMM:NF013962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOD99027.1"
                     /translation="YDRPGVIGNIGSTLGANHINIARLHLSREEADQEALVILSTDGS
                     VPEEVMDKLLKLPHVISVKDVEM"
BASE COUNT          977 a         1643 c         1540 g         1224 t
ORIGIN      
        1 cgccgccgta ctcctcgggg atccaggccg ccaccaggcc gttttccgcc gcttttttcc
       61 gtacttcagg ggtgtatttc tcgtgctcgt cgcattcctt gctgatcggt gtaaactccg
      121 tcacggcgaa cttgtaggcc atgtccttca acatcttgag ttcttcggtc aattcaaaat
      181 ccataattgt ttctcctttt caaattgatg tgaaaacggg gacggtcccc attttttatt
      241 gtcgtatccg ccactataaa caagagcttg aaaacgagta ccgtcgtcat ttatttaaaa
      301 aacgctaatt ttaaagacct gacccccaaa gcagcctcaa tagtcgtaca cgcccttacc
      361 ggtcttgcgg ccgaagcggc cggcccggac gagtttccgg aggagtggcg cggggcggta
      421 cttgtccccg agttcccggt ggaggccttc gatgaccctc agcagcgtct cgttgcccac
      481 gaggtccgac agggccagcg ggccgatcgg atggttgcag cccaggacca tggccttgtc
      541 gatgtccgcc gccgaggcga tcccctcgtc caggacgaag aaggcctcgt tgagcatcgt
      601 gcagaggatc cggttgaccg cgaaggccgg ggcttccttc accaccacga cctccttgcc
      661 gatcttcttg ccccacgccc tggcgacttc cactgtctcg tccgaggtct catggccgcg
      721 gatgacctcc aggagcttca tgaccggaac gggattgaag aagtgggtgc cgatgaactt
      781 gtcgggacga tccgtgatgg cggccatctc cgtgatgctc aggccggagg tgttggtgaa
      841 aaagtaacag tccttggaaa ccacggccat cagttccttg taaacctgtt tctttacttc
      901 caggacctcg atgaccacct ccaccaccag gtcggccccc gccgccgcct ctttcaggtc
      961 cagcgtcggc ttgatccgcc cgaggatggc gtccatatcc tctttggtcc gtttgccctt
     1021 ttccacgtcc ttggagaggt tccgggtgat ggtcttcatc cccccgtcga tgaaccgctg
     1081 ctcgatgtcc cgcatggcca cttcgtagcc cgcctgggcg cacacctggg tgatgccatt
     1141 gcccataagg cccgccccca aaacgcaaat ctttttaatt tccatacttt acttcctcct
     1201 ttatgatttg gaaaaattta aaatttaggg acacttccca tatttttaag ccgccttcat
     1261 atcgctccca ggaaccactg cttattcagc cagtgcatct tcttcgcctc ttccagcagc
     1321 tcgtcccgga agtccgggtg ggccagcttg gttatcgcca atacccgctc ggggatggag
     1381 aggcccttca gataggcgac gccgtgctcc gtgaccagat aggacacgtc cacccggggc
     1441 accgtgacga tctcccccgg cggcagggtc ggcgtgatct tcgatttcag tttcccctcc
     1501 ttgtccgtgt aagtggactg gaaggccagg acggacttgt tctccggata ccccagcggc
     1561 ggctgcccct tggtggcccg gtaataagcc ccccggacga aggccaactg tcctcccgtc
     1621 cccgagtact gggcgaagcc gagggattcg gagctcacct gaccctggag atccaccccc
     1681 aggcaggcgt tgatggagat caggtccttg ttcatggcga tgatgttggg atcgttggtg
     1741 aattccacgg gatacatggc cagcgccggg ttgtggtcga tccagtcgta gagccggcgg
     1801 ctgccgatgg cgaaggccca gacgatcttg ccgggaagga gggtcttctt ctttccggtg
     1861 atgaggcccg catcgtggag atcggcgatt ccttccgtga ccatttcggt atggatgccg
     1921 atgtcccggg cgccgctgtc cgccaggagc cgcaacaccg tgtccggcag gccgccgatg
     1981 cccacctgga gggtgggccc gtccttgggg atcaactcgg cgatgtaacc ggcgatcttc
     2041 tcttccaccg ctgtcggctg gggggtgggg atggtgatca acttgtccgt gttgttctcg
     2101 atgacgtaat cgacttcgga gatatgaatg cattcgtcgt agccgccgta acaccagggc
     2161 atatcctcca cgacctcgac gacgatgatc ttggcccgtt cgaaaacggc cttcttgaag
     2221 ttgcacaggg gaccgaagtt gaagtacccg tgcttatcca tgggggtgac gatctggacg
     2281 gcgatgtcca cgtcgaggcc gtcccggtaa tagcgtgccg cctcgccgaa attgtagggg
     2341 atgtgggtcc cccggttttc ggcgatgtac ttccgcgtcc agcccaggat gtgccagttg
     2401 ccgatccaga agctcttcgc ctccgggtcc ttctccatga cctccagggg cgtcagatga
     2461 cccagggcgc ggatcttgac gttcttcact tcgccgaccc gctgggccag cgcgcgatca
     2521 aaggcccggg gcgcctgcac cgtgccgtag tccacccaca tgccgtcccg aaccagggaa
     2581 gcgacctgct cgggcgtggc taacttgttt ccgtactttg acatgcagct acctccttta
     2641 ttaagggggc cgggtcttgc attttagcgt ttttggcaaa acctcactgt aacgaaattc
     2701 agcgaagctg atggatgttc atgattaccg acgaatttcg ggccggcggc gggatgtaga
     2761 tatcggtttg ccggcctcga taggcctgta tgtatgccac atccaaacta tttgcaaacg
     2821 ttttattgcg aaaacccacc ccggattagc gtgtcggaaa aggtggggct gcctgctatt
     2881 tagcaattat tgtgccaggg aataaaagtt caaatatcgt gcaaaaatac ggggagttaa
     2941 taaaacggcg gcggagggga tgggcaaata cgccttatgc aatcccgcat acatatgcat
     3001 tgttgcgcat catggatgag ggatgaggca aaaatggggg cggttcttta ccccgaagtt
     3061 ttgatccctc gcggcttgtt gcgaggaggt ttgcttgcct tgccggcact cacgaatgcc
     3121 gcgcccgacc gtggtgatcg cgtcccgcgc gcaaggagtt tacgcacaat gatgtggtgg
     3181 aggtgctgga ctcgtcgatt gcttttcgtg agcaaggagt atccccccgg atatcaggtg
     3241 acggcatcgg caatgttgtc cgtaggcggc atcggcaagc ttatccgcgg gtggcatcgt
     3301 caatgttgtc cgcttcggtc gggacgagtc ttacttcttc ttgtggcggt tggccttgag
     3361 tctctttttg tacttgtgct tgctgatctt cttttttctc ttctttacga cgctgcccac
     3421 tattaacccc ttcctcgcta attttgagtg gtgggacgtg cgggaatcga acccgcgacc
     3481 aacggattaa aagtccgctg ctctaccgga ctgagctaac gtcccgtctg ctcactgaaa
     3541 gcctgcctac atatcagcat cggcgccgaa ttgtcaagaa tttataaaat atgggtacgc
     3601 ttcccgtatt tatgatgaga aggggttctt cagaatgatg gtttcattcc gcgccgcccc
     3661 cacggatacg aggacgagag ggacgtccgc cagggtctcg atccgctcca ggtatttccg
     3721 tacgttcttc ggcaactcct cgaggtgtct ggcctggctg atgtcctccg tccagccctc
     3781 caactcctcg taaaccggct cgcatcccgc cagcgtccgg agatcggcgg ggaccgccag
     3841 gttgatctgc tctccctccg ctgtccggta gcctatacag atcttgatct tgtcgatgcc
     3901 cgtcaggacg tccagcttgg tcagggcgat ccccgtgacc ccgccgaccc gtacggagtg
     3961 gcgcaccagg accatatcca gccagccgca ccgccgccgc cgccccgtcg tggcgccgaa
     4021 ttccttgccc acctcctgga gccgccgccc gatttcatca tccagctccg tggcgaaggg
     4081 gccgcccccg acccgggtcg tataggcctt gcagatcccc acgacggcga aggccgccga
     4141 cggccccacc cccgtgccgg aaaaggcgtt ggcggacacc gtgttggacg aggtgacgaa
     4201 gggataggtg ccgtgatcta tgtcgaggtg gcagccctgg gccccttcga agaggatccg
     4261 cttgccccgc cgcaactccc ggtagaggat gacggaggtg tcggccacat attcccgcag
     4321 gcgctcgccg taggcccggt gttcctcgta aaccgcctcg gggtccaccg gcgcctcgcc
     4381 gaagtacttc gtcaggagga aattcttctc ctccaggttg cggcgcagct tctcccggaa
     4441 ggcgtcgccg tcgcccgtca gttcacccac ccggatcccc gtccgggcca ctttgtcctc
     4501 gtaggcgggg ccgatgccac ggcccgtcgt gccgatcttt ccgccgcccc gtttgccttc
     4561 ccgggccagg tcgaggcgtt tgtggtaggg catgatgaga tgggccttct cgctcacgta
     4621 cagttgggtc tgagggggaa aaagtccccg ctcctccaac gcgtcgatct ccgtgatcag
     4681 gacccgggga tccaccacga cgccgttgcc gatgatgcag agcttctgat catggaggat
     4741 tcccgagggg acgaggtgga ggattgtctg cacccccttg acgaccaggg tgtggccggc
     4801 attgttgccg ccctgaaaac gggcgatgac gtcggcgttc tcggcgtaca ggtccacgat
     4861 cttgcccttg ccctcatccc cccactgggt tcccaccacg atcacgttgg ccatgagtca
     4921 tctccttttc ccgaataatt caaaattgcc caaaacccat ctccacccct ccccttgaag
     4981 gggagggtga tttgcgactc tttacggggg catcaaattt tgaccaagaa tctttaatga
     5041 ccgccccttc gggcggcttc gcaaaaagag ccgcaggcgc ggcgcacggc acctgagaag
     5101 cggaggcgta ctctttgtac gtcgagcgac gaaggcggcc gcgcaacaaa gcatccggcc
     5161 ttttttccgg agccgcctct acatctctac gtcttttaca gaaataacgt ggggcagttt
     5221 caggagcttg tccatgacct cctcggggac ggagccgtcc gtgctgagga tgaccaaggc
     5281 ctcctgatcc gcttcttccc ggctcaggtg cagccgggcg atgttgatat ggttcgcccc
     5341 cagggtcgag ccgatgttgc cgatgacgcc ggggcggtcg taat
//
LOCUS       DAOSMF010000131         5383 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_16671_length_5383_cov_18.475601, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000131 DAOSMF010000000
VERSION     DAOSMF010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5383
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16671_length_5383_cov_18.475601"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1553)
                     /locus_tag="PLT63_09660"
     CDS             complement(<1..1553)
                     /locus_tag="PLT63_09660"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOD99028.1"
                     /translation="MATTAPVKDPRFRIRTKMLLVLLGISLIPLIVFGYIARQDIEEV
                     SRYTVKASNAIGERASQDSAKALEDLGAGMIRQKAIDVALQCAIYLRRHPGVPIKKFQ
                     MDKEFQQIAVQSVGVTGYTIIFDRKGVMLFHPNRELINYDMHNWREMLPGFWALFEQS
                     FDGSSGGGYYNWQDSDRVIRRKFMYIAPIQETQYMVAATTYIDEFSRPAEETKERIAT
                     ATANIREEINRSIEGISSSFIAIMASMLLIVAAISFLLSRMITNPILALMRGAKAIGR
                     VEMEHTVEVKTGDELEVLADSFNKMARDLKWHMEDLKCTTAEKERLQKELEIARGIQL
                     RLLPQHPPYIEGMDLVANNIPAQEIGGDFYDYIPISRGSWGLVIADVSGKGMPAAIFM
                     GLSRTIVRASATDNLSAAAAIKQANELICRDSTSGMFVTLFYAILDVKERKLRYVNAG
                     HNPPLLIRNGGEVELLRARGIALGVTHYIDLQEAERTLQPGDTIVFYTDGVTEAINAE
                     EEAFGERRL"
     gene            complement(1532..2488)
                     /locus_tag="PLT63_09665"
     CDS             complement(1532..2488)
                     /locus_tag="PLT63_09665"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOD99029.1"
                     /translation="MTNSIRSGKIRVEKYLRATVLNDVVELEKRVWKKEGYREVNAPM
                     VYFELAYLTNGLILTAFDEGVYSREERESMAKEDPWYEGDRLIGFAALFADFDHNGPF
                     WYGARMGVDDRYQDKNVGDAMVNALYECAKERNIPRIRWTYDPLQSRNGYIYLRKMGG
                     LVKEIGFNYYSAVFTNDHFNRGISTDRFIVEWMIGSERVRERMEEGRIPPADAAAITI
                     ENTINEIQIGADGLESHGERWLFQSIQSPLFIEIPYNQDRLLKTDRDRAQALRDKCRA
                     LFMHYLARGYVVNDLIVKQSPDGRRHAYYRLDQDIQWQRLRL"
     gene            complement(2518..2991)
                     /locus_tag="PLT63_09670"
     CDS             complement(2518..2991)
                     /locus_tag="PLT63_09670"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD99030.1"
                     /translation="MKTITMSFFQIILPANINHLSDIIEDLSRWMEQQGVMRDRIVRV
                     QLAVEEALVNVIRYAYQGKEGEVELQGEIADDQRLVIKILDNGTPFDVCTVPHPDISC
                     GIRDRKIGGLGIYLIHKMVDELCYRREGDRNVLTMFIRWSNGNLPRSRQDAHAGA"
     gene            complement(2988..3320)
                     /locus_tag="PLT63_09675"
     CDS             complement(2988..3320)
                     /locus_tag="PLT63_09675"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOD99031.1"
                     /translation="MEIDVRKEGQAVIISLTGRMDAVSAPQFDRTAEDLLAQGDRRII
                     IDFVALEYISSAGLQSILALAKRLEPVDGDVILCNLGGPVKEVFDISGFSIIFPIFDS
                     LADAKASG"
     gene            3506..4294
                     /locus_tag="PLT63_09680"
     CDS             3506..4294
                     /locus_tag="PLT63_09680"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF006070.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HOD99032.1"
                     /translation="MKLQDMFKLNGKTALVTGGGRGIGRFIATGLAEAGADVIVTSRK
                     MKNLEATAQELAASYGVRAYALPCDLGKEEDIERLVGEALDKCGRIDILVNNAGATWG
                     APTLDFPLERWDQVFNINIRGVWILTQKVARIMKNQGGGNIINISSVMGFRGSKEAVH
                     PAVPYNSTKAAINVLTMNLAVKLAPYNIRVNAIAPGFFRTDMMAYIEKPEFKAAHDAI
                     VNSIPLRRVGDQDDMKAVAVFLASDASAYLTGHIIINDGGLLAK"
     gene            complement(4419..4997)
                     /locus_tag="PLT63_09685"
     CDS             complement(4419..4997)
                     /locus_tag="PLT63_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOD99033.1"
                     /translation="MSKGKMILVGVLVAFLLVALSLYGFFKGTYNQFVTLDEGVKSAW
                     AQVENQLQRRFDLIPNLVETVKGYAKQEKDVLVEVTNARARVGGANTVPEKIEANNEL
                     SGALSRLLVVVEKYPELKSNENFLRLQDELAGTENRIAVERKRYNDAVKNYNVAIRSF
                     PANLLAGMFGFDKAAFFEAPVAAKATPQVKFQ"
BASE COUNT         1086 a         1628 c         1498 g         1171 t
ORIGIN      
        1 atgagccgcc gctcgccgaa ggcctcctct tcggcgttga tcgcctccgt caccccgtcg
       61 gtatagaaga cgatggtatc ccccggctgc agggtccttt cggcctcctg gagatctatg
      121 taatgggtga cccccagggc gatgccccgg gcccgcagca attccacctc gccgccgttc
      181 ctgatgagca gcggtgggtt gtgaccggcg tttacatagc gcagctttct ctccttgacg
      241 tccaggatgg cgtagaacag ggtgacgaac atcccggagg tggaatcccg gcagatcagc
      301 tcgttggcct gcttgatggc ggcggcggcc gagaggttgt ccgtggcgct ggcgcggacg
      361 atggtgcggg acagtcccat gaagatcgcc gcgggcatcc ccttcccgga aacgtcggca
      421 atgaccaggc cccatgaccc gcgcgagatg gggatgtagt catagaaatc gccgccgatc
      481 tcctgggccg gaatgttgtt ggccaccagg tccatgcctt cgatataggg cgggtgctgg
      541 ggcagcaggc gcaactggat gccccgggcg atttccagtt ccttctggag ccgttccttt
      601 tccgccgtgg tgcacttgag gtcctccata tgccacttga ggtcccgggc catcttgttg
      661 aacgaatcgg ccagcacttc cagttcgtcg ccggttttta cctctactgt gtgctccatt
      721 tccacgcgcc cgatggcttt ggcacccctc atcaacgcca ggatgggatt ggtgatcatc
      781 cgggaaagca gaaaggaaat ggcggcgaca atcagcaaca tggacgccat gatggcgatg
      841 aagctgctgc tgatcccctc gatactcctg ttgatttcct cacggatgtt ggcggtggcc
      901 gtggcgatcc gctccttcgt ctcttcggcg gggcgggaga attcatcgat ataggtggtc
      961 gccgccacca tgtattgcgt ctcctggatg ggggcgatat acatgaactt ccggcggatg
     1021 acgcggtccg aatcctgcca gttgtaatat cctccgccgg aagaaccgtc gaaggactgc
     1081 tcgaaaagtg cccagaagcc cgggagcatc tcccgccagt tgtgcatgtc gtaattgatc
     1141 aactcccggt tcggatggaa aagcatgacc cccttgcggt cgaaaataat agtataaccg
     1201 gttacaccca ccgattgcac ggcgatttgt tgaaactcct tatccatttg gaattttttg
     1261 atgggtacgc cgggatggcg tcgcaggtag atcgcacatt gcagggccac atcgatggcc
     1321 ttctgccgga tcatccccgc gcccagatcc tccagggcct tcgcactgtc ctgggaagca
     1381 cgctcgccga tcgcattgct ggctttaacc gtatagcggc ttacctcctc gatatcctga
     1441 cgagcgatat aaccgaagac gatgaggggg atcagcgata tcccgaggag caccaggagc
     1501 atcttggtcc ggatgcggaa ccggggatct ttcacaggcg cagtcgttgc cattgaatgt
     1561 cctgatccaa tcggtaatag gcgtgccggc ggccatccgg cgattgtttg acgatcaagt
     1621 cgttgaccac gtaacctcgg gccagatagt gcatgaacaa tgcccggcat ttgtcccgca
     1681 gggcctgggc ccggtcgcgg tcggtcttga gcaggcgatc ctggttgtag gggatttcga
     1741 tgaacagagg actctgaatg gattgaaaca gccagcgttc gccgtggctc tccaagccgt
     1801 ccgccccgat ctggatttca ttgatggtgt tctcgatggt gatggccgcc gcgtccgccg
     1861 ggggtatccg cccctcttcc atgcgctccc ggacccgctc cgagccgatc atccactcca
     1921 cgatgaaccg gtcggtggag atgccccggt tgaaatgatc gttggtgaaa acggcgctgt
     1981 aatagttgaa cccgatttcc ttcaccaaac cgcccatctt ccggaggtag atgtagccgt
     2041 tccgggactg cagggggtcg taggtccagc ggatccgcgg gatgttccgc tccttggcgc
     2101 actcgtagag ggcattgacc atggcgtcac ccacgttctt gtcctgatag cgatcgtcca
     2161 cacccatccg ggcgccgtac cagaaaggtc cgttatggtc aaaatctgca aagagagcgg
     2221 caaagccgat gagccggtcc ccttcgtacc agggatcctc cttcgccatg gactcccgct
     2281 cctctcggga atatacccct tcgtcgaagg ccgtcagaat gaggccgttc gtaagatatg
     2341 ccagctcgaa atagaccatc ggggcgttga cctcccggta cccctccttt ttccagaccc
     2401 gtttctccag ctctacgacg tcgttgagaa cggtggcgcg gagatatttc tcgacgcgta
     2461 tcttcccgct tctaatgctg ttcgtcatcg tgctgcagcg tccccctttc cctttcctca
     2521 ggcgccggcg tgggcgtcct gccgcgatcg cggcaaattg ccgttgctcc atctgatgaa
     2581 catcgtcaac acatttctat ctccctcccg gcggtagcaa agttcatcga ccatcttatg
     2641 aatcagataa atccccagac cgcctatctt gcgatcccta ataccgcagc ttatatccgg
     2701 atggggaacg gtgcagacat cgaagggcgt cccgttgtcc aggatcttga tgaccaggcg
     2761 ctgatcgtcg gcaatctcgc cctgcaattc cacctcgccc tccttcccct gatacgcata
     2821 acggatcaca ttcaccagtg cctcttctac ggccagttgc acccgtacta tcctgtccct
     2881 catcacgccc tgctgctcca tccagcggga cagatcctca atgatatcgg atagatgatt
     2941 gatgttagcc ggaaggatga tctgaaaaaa ggacatggtg attgttttca tccggacgct
     3001 ttggcatccg cgagactgtc gaaaatggga aatattattg aaaaaccgga gatatcaaat
     3061 acctctttta ccggaccgcc gagattgcag aggatgacgt cgccgtcgac cggttcaagc
     3121 cgtttcgcca aagcgaggat gctctggagt ccggcgctgc tgatgtactc cagggccacg
     3181 aaatcgatga ttatccgccg gtcgccctgg gcaagcagat cttccgcggt cctgtcgaac
     3241 tggggagcgg atacggcatc cattcttccc gtcaacgaga tgatgacggc ctgtccttct
     3301 tttctcacat cgatctccat ggcaccctcc gcagcgagct ttgtccggac ctgacacggg
     3361 aagaaagtaa catataagcc gcgggccggt caagatatag ttgggtgatt taattgcctt
     3421 gacatggtgt tacgcgggat ttataaggtc cccaacggcg ggagggacga aggccgggaa
     3481 aacatcattg cgaaggagaa accgcatgaa gcttcaggac atgttcaaac taaacggcaa
     3541 gacggccctc gtcaccggcg gcggccgggg tatcggcagg ttcatcgcca cgggacttgc
     3601 cgaggcggga gcggatgtga tcgtcacgtc acgaaaaatg aagaatctcg aggcgacggc
     3661 ccaggaactg gcggcgagtt acggggtccg cgcctatgcc ctcccctgcg atctcggaaa
     3721 ggaagaagac atcgaacggc tggtcgggga agccctggac aagtgcggcc gcatcgacat
     3781 cctggtgaac aacgccggcg ccacctgggg cgccccgact ttggatttcc ccctggagag
     3841 atgggaccag gttttcaaca tcaatatccg cggggtctgg attctcaccc agaaggtggc
     3901 ccggatcatg aaaaaccagg gcggcggaaa catcatcaac atctcttccg tcatgggctt
     3961 ccggggctcc aaggaagcgg tccatcccgc cgtcccctac aactccacca aagcggcgat
     4021 caacgttctg accatgaacc tcgccgtgaa actggccccc tacaacatcc gggtcaacgc
     4081 catcgccccg ggcttcttcc ggacggacat gatggcctat atagaaaaac cggaattcaa
     4141 ggccgcccac gacgccatcg tcaactccat ccccctgcgc cgcgtcggcg accaggatga
     4201 catgaaggcg gtggccgtct tcctggcttc cgacgcctcg gcctatctga ccgggcacat
     4261 cattatcaac gacggcggcc tcctggccaa gtaaaaaatg gggactgttc tcattttcgc
     4321 accttaacag accatcttcc ccctcccctt caaggggatg aatggggagg ggatgggata
     4381 aggattttca atttctgcaa gcaggctact tccccgcttt actggaactt cacctggggc
     4441 gtcgccttcg ccgccaccgg ggcctcgaag aacgccgcct tgtcgaagcc gaacatcccg
     4501 gccaggagat tggcggggaa gctgcggatg gccacgttgt aattcttcac cgcatcgttg
     4561 tagcgcttcc gctccacggc gatgcggttt tccgtccccg ccagctcatc ctggaggcgc
     4621 aggaagttct cgttggattt gagctcggga tatttttcca cgaccaccag caggcggctc
     4681 aaggcgccgg acagctcgtt gttggcttcg atcttttcgg ggacggtatt cgccccgccc
     4741 acccgggccc gggcgttggt cacctccacc aggacgtcct tttcctgctt cgcgtagccc
     4801 ttgactgtct cgacgaggtt ggggatcaga tcgaagcgac gctgcagttg gttttccacc
     4861 tgcgcccagg cgctcttcac cccctcgtcg agggtgacga actgattgta ggtccccttg
     4921 aagaaaccgt aaagcgacag ggccacaagc aggaaggcca ccagtacccc aaccagaatc
     4981 atctttcctt tggacatctc tttacctccg ttcatataaa aatgcgttcc tatatatttg
     5041 tcttcgttca gtaattatag ttcgtcgcca cctaaccgat tttgaatcgg ccggcaataa
     5101 cgactccgtc agacgcttac ataaaaaaac gcctttcgtc attttcgcgc cacgggaatc
     5161 cagctttttc cggcatcttc cggatgccgg ttcgggcccg ccccggacca cgatccggga
     5221 cacggcatga caagacaggt tgttccgcaa agctctcatt ttatcatcgt gtgcaggctt
     5281 cctcaccccg ccgtgggaaa ggacagctcg tcgatacgcc ggcagagccc ggcgatctcc
     5341 cgcaggtatt cccggagcag cgccatgatc tcgcccgggg aat
//
LOCUS       DAOSMF010000132         5235 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_17252_length_5235_cov_23.288224, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000132 DAOSMF010000000
VERSION     DAOSMF010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5235
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17252_length_5235_cov_23.288224"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="PLT63_09690"
     CDS             complement(<1..690)
                     /locus_tag="PLT63_09690"
                     /inference="COORDINATES: protein motif:HMM:NF015949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HOD99034.1"
                     /translation="MTMKKPGLLARSLRDLLYKEARIRTVAGVCIGSTYIAVRLDDDG
                     LGLAAVPVAALSGPEQEMSFPPEASLRGRPAAFLLDWLTQSDHPRQKALALATANALI
                     RQDHSDFDGDALDAFHLTADDRVVMVGRFTPLMARITATGAALTVLEKNPAKGMVLPA
                     EERRRVLERGTVAIITATAMLYDDLEEILSDLVRPRHVSLLGPSTPMLPEIFALTPVT
                     HLGGVKITDTDR"
     gene            856..2106
                     /locus_tag="PLT63_09695"
     CDS             856..2106
                     /locus_tag="PLT63_09695"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOD99035.1"
                     /translation="MFEYLLTKEQLAIRDEARELVKWVPRQMILDMDQDRIRFPKEFL
                     AEAGRRNLLGCRYPRQWGGREMDWVTTAAILEEIGTLGYEFACVFGVGAELVCDAIIL
                     HGTDHLKEKYVRPLLRGEIFAAECLTEPRGGSDFFGATTKAEDRGDHYLLNGQKRFIV
                     GGEGADYFLVYARTNFDPAAKPQDCLTAFVVDRGPGVETKYLYGLMGCRGGGTARIVF
                     KNVMVPKENVVGQVNGAYAVFNTMMIPERLGTAMMTIGAARPAIEIATKYTARRKAFG
                     KIIATFEGVSFQVAEAVMLLDACRSMAYTTARAVETGQEMLRIRRLVSETKKFITESC
                     QKVVHNAMQVVGGIGYTNVFPLERIYRDIRLASIWTGTSEVMSMITAHEWYREFLGKE
                     IRTRPRDHEADAEEAFAAEEKIYE"
     gene            complement(2237..3559)
                     /locus_tag="PLT63_09700"
     CDS             complement(2237..3559)
                     /locus_tag="PLT63_09700"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD99036.1"
                     /translation="MNGGSILEGRDIQVRRGGRLILDVASLNIAAGEVLSVIGPNGAG
                     KTTLLQALSVLLRLNRGEIRFRGARIGVDITPLDYRRQLAMVFQEALLFDATVTANVA
                     AGLRLRGTDAGEAKRIVAAKLEQFGISSLHDRSAKTLSGGEAQRTSLARAFAIGPQIL
                     FLDEPFAALDPPTRESLIEDLSRILRESGATAIMATHDQGEALRLSDRIAVMHEGRIA
                     QIGPPVEVMNHPVDESVAAFVGVEMILAGRVVQYERGTATVAITNDVGPPKLSYPRGN
                     PGEILSGTGRERTAGEAPGERSSASLSSDGMEMPPEATGGRIDGEGSCVWEDAAVMGG
                     RRRPATVFLEAAGEAKAGEEVFCCIRPEHVTLARGLPPGRTSARNSFPAIIDRIIPAG
                     PFYKLYLDGSLPLSAYITAQSLEELDLRIGMTVTASFKATSVHLIRRG"
     gene            complement(3556..4254)
                     /locus_tag="PLT63_09705"
     CDS             complement(3556..4254)
                     /locus_tag="PLT63_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOD99037.1"
                     /translation="MEIILAGIKQALSLLISFDPEVLAITWLSLKVSGTATLISLFIG
                     IGIGTAVALSSFPGRQIVVSVINTGMALPPVVVGLFVTIFLWRNGPFGYLEILYTPAA
                     IIIAQAIIATPIVMGITLAAMQQLPRKLRLQILALGATRFQMVWLLIKEARLSLMAAV
                     MAGFGGVISEVGASIMVGGNIKGYTRVLTTATVMETSRGNFDLAIALGVILLLFAFVI
                     NLVLTRIQQRERPQ"
     gene            complement(4428..>5235)
                     /locus_tag="PLT63_09710"
     CDS             complement(4428..>5235)
                     /locus_tag="PLT63_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOD99038.1"
                     /translation="GGADAARKAPAKQKNLILATTTSTQDTGLLDVLIPIFEKKTGYF
                     VKTIAVGSGQAMTMGSKGEADVLLVHSQAAEKKFMEAGNGVNRRLVMHNDFIVVGPPA
                     DSVALKGTKKATEGFKKISAANALFVSRGDNSGTHAKEKEIWKAAGIQPEGQKWYQQT
                     GLGMGQTLSVAAEKKGYTLTDRGTYLALKKNLGMDSLLEGDAVLLNIYHVIEVNHAKW
                     PKVNAAAAKAFADFMVSKEVQKIIKTFGVAKFGSPLFFPDAGKKVEDLGK"
BASE COUNT         1018 a         1591 c         1647 g          979 t
ORIGIN      
        1 tcggtcggta tcggtaatct tgactccccc caaatgggtc accggggtaa gggcaaaaat
       61 ttcgggcagc atgggggtgg aggggcccaa aagggaaaca tggcggggac ggacgagatc
      121 ggagaggatc tcttccagat cgtcgtacag catcgcggtg gccgtaatga ttgcgaccgt
      181 tcccctttcc agaacgcggc ggcgctcttc ggccggaagg accattcctt tggcggggtt
      241 cttctccaga accgtcagcg cggctccagt ggcggtgatt ctcgccatca ggggggtaaa
      301 gcggccgacc atgaccactc tgtcgtcagc ggtcagatga aatgcatcga gggcatcacc
      361 gtcaaaatcg ctgtgatcct ggcggatcag ggcattggcc gtggccaggg cgagggcctt
      421 ttgccgcggg tgatcgcttt gagtcagcca gtccagaagg aaggccgccg gccggccccg
      481 cagggatgct tccggaggaa acgacatctc ttgttcgggc ccggataatg ccgcgaccgg
      541 cacggcggca aggccgaggc cgtcatcgtc cagccggacg gcgatgtagg tcgagccgat
      601 gcatacaccg gcgacggttc tgatccgcgc ctctttgtag aggagatcgc ggagacttct
      661 ggccagtagg ccgggttttt tcatggtcat aacagactcc cctatgatcg actgattcta
      721 ccgcagtggc gggtgctgtc aacatcaaat cccgaggggt cgttttttcc tcccttgaca
      781 ggtttcgcgc atctccctat actccgcctg ccccgccgcg ggaacagtga gggctttaca
      841 ggaaaggagt tcgtgatgtt cgaatatctg cttaccaaag agcaactggc catccgggac
      901 gaggcccggg aattggtcaa atgggtaccc cgccagatga ttctcgacat ggaccaggac
      961 cggatccgtt ttcccaagga gtttctcgcc gaggcgggac ggcggaacct gctgggctgc
     1021 cggtatcccc ggcaatgggg aggacgggag atggactggg tcaccaccgc cgcgattctg
     1081 gaggagatcg gcaccctggg gtacgagttc gcctgcgtct tcggcgtggg agcggagttg
     1141 gtctgcgatg ccatcatcct ccacggtacg gaccatctga aggaaaaata tgtccggccc
     1201 ctcctgcggg gagagatttt cgccgcggaa tgcctcacgg agccccgggg cggttcggat
     1261 ttcttcggcg ccactacgaa ggccgaggac cggggggacc attaccttct caacggccag
     1321 aagcgcttca tcgttggcgg ggaaggggcg gattatttcc tggtttacgc ccggaccaac
     1381 ttcgatcccg ccgccaaacc ccaagactgc ctcacggcct ttgtcgtgga ccgggggccc
     1441 ggggtggaaa cgaagtatct ctacggcctc atggggtgcc ggggaggcgg cacggcccgc
     1501 atcgtcttca agaatgtcat ggtccccaag gagaatgtcg tcggccaggt caacggggct
     1561 tacgccgtct tcaacaccat gatgatcccg gaacgccttg gaacggccat gatgaccatc
     1621 ggcgccgccc ggccggccat tgagatcgcc acgaaataca ccgcccggcg caaggccttc
     1681 ggtaaaatca tcgccacctt cgaaggggtg agcttccagg tcgccgaagc ggtaatgctc
     1741 ctcgacgcct gccggtccat ggcctacacc accgcccggg ccgtggagac ggggcaggag
     1801 atgctccgga tccggcgcct ggtctcggag acgaagaaat tcatcaccga gtcctgccaa
     1861 aaggtcgtcc acaacgccat gcaggtcgtg ggcggcatcg gttacaccaa tgtattcccc
     1921 ctggagcgga tttaccggga catccgcctg gccagcatct ggaccgggac gagcgaggta
     1981 atgtccatga tcacggccca cgagtggtac cgggagttcc tgggcaagga gatccggacc
     2041 cggccccgag atcacgaggc ggacgctgaa gaggccttcg cggcggaaga gaagatctac
     2101 gaataaccgg gcggaaagcc ccggaccgga tcggaacacg ggttaggcgg ggcttgggcc
     2161 tgattgcgga ttttatgtcc agcaaaggca tccgtggcat ccgggaccgg ggatgcgagg
     2221 ccccggcggg ggatcactat ccccgccgga tcaaatggac ggaggtggcc ttgaaggaag
     2281 ccgtaaccgt catcccgatt cgcaaatcca gttcttccag ggattgggcg gttatgtacg
     2341 cggacagggg aaggctgcca tccaggtaca gcttgtaaaa agggccggcg gggatgatcc
     2401 tgtcgatgat ggcggggaag gagttgcgag cgctggtccg ccccgggggc aaccccctgg
     2461 cgagggtgac gtgctccggg cggatacagc agaacacttc ctcccctgct ttcgcctctc
     2521 ccgcggcctc cagaaacacg gtggcgggac gtcgccttcc ccccatcaca gcggcgtctt
     2581 cccatacgca agaaccctcc ccgtctatcc gtccacccgt agcttccggc ggcatctcca
     2641 tgccatcgga tgataacgag gccgatgatc tctcaccggg cgcttcccct gccgttctct
     2701 cccttccggt cccggagagt atctcgccag gattgccccg agggtaagat aacttcgggg
     2761 ggcccacatc attcgtgatg gccacggtgg ccgtcccccg ttcgtattgg acgacccggc
     2821 cggcaagaat catttccacc cccacgaagg ccgccacact ctcatcgacg gggtggttca
     2881 tcacctccac cgggggaccg atctgagcga tccgcccttc atgcatcacg gcgatccggt
     2941 cggaaagacg cagggcctcc ccctgatcat gggtggccat gatggccgtg gcgcccgatt
     3001 cacggaggat gcggctgagg tcttcgatca gggattcccg ggtgggtgga tcgagggcgg
     3061 caaagggctc gtccagaaaa aggatctgcg gcccaatggc aaaggcccgg gcaaggctgg
     3121 tccgttgggc ctcaccaccg gagagggtct tggcggagcg gtcgtgaagg gaactgatgc
     3181 cgaactgctc cagcttagcg gcgacaatcc tttttgcttc cccggcgtca gtcccgcgga
     3241 gccgaagtcc cgccgccaca ttggcggtta ccgtcgcatc gaagaggagg gcctcctgga
     3301 agaccatggc caactgccgg cggtaatcca gaggggtgat atccacgccg atccgggcgc
     3361 cccggaagcg aatctcccct cgattgagcc gcaggaggac cgacagggcc tgaaggagcg
     3421 tcgttttccc cgccccgttg ggcccgatca cggacagcac ctctcccgcc gcgatgttca
     3481 gagaagcgac atcgaggatc agtcggccgc cccgccgcac ctggatgtct ctgccctcca
     3541 gaatcgatcc gccgttcatt ggggacgttc ccgctgctgg attcgggtga gaaccaggtt
     3601 gatgacaaag gcgaagagaa gcaggatcac ccccagggca atggcgagat cgaaattgcc
     3661 cctgctggtt tccatgaccg tggcggtggt caggacacgg gtatagccct tgatgttgcc
     3721 gccgaccatg atggaggccc cgacctcgga gataacgcct ccaaagccgg ccatgacggc
     3781 ggccatgagg gacagtcggg cctccttgat aagcagccag accatctgaa agcgcgtcgc
     3841 tcccagggcc aggatctgca accgaagctt ccggggcaac tgttgcatgg cagccagggt
     3901 gatacccatg acaatggggg tggcgatgat ggcctgggcg atgatgatgg ccgcgggcgt
     3961 gtagaggatc tccagatagc cgaaaggacc gttgcgccag agaaagatcg tgacgaaaag
     4021 gcccacgaca acaggcggca gggccatccc cgtattgatg acgctcacga caatctgccg
     4081 cccggggaag gaggagaggg ctacggcggt gccaatgccg atgccgataa acaggctgat
     4141 cagggtggcg gtccccgaaa ccttcaggga cagccacgtg atcgccagga cctcggggtc
     4201 gaagctgatc aggagggaga gggcctgttt gatcccggcg agtattatct ccattcccgc
     4261 ctctcccccg tcggcgctat ccccacggac atgaaagtat ccaccctttg ccggtttttt
     4321 tcaccggctg aaaacatccc gtctcccacc cccggctcga acggatgcgg cggcggattt
     4381 aaaaccgctc gcgcgccgac atatccagat gaaaggcgtc ccacagatta tttgcccaga
     4441 tcttccacct tcttgccggc atcggggaag aacagggggg atccgaactt cgccacgccg
     4501 aaggtcttga tgatcttctg gacctctttg gacaccatga aatcggcaaa ggccttggcg
     4561 gcggcggcgt tcaccttggg ccatttggca tggttgacct cgatgacatg atagatattg
     4621 aggagcaccg catccccctc gaggaggcta tccatgccaa ggttcttctt cagcgctaga
     4681 taggtgccgc ggtcggtaag ggtataaccc ttcttttccg cggccacgct aagggtctgt
     4741 cccatcccga gtcccgtctg ctggtaccat ttctgcccct ccggctgaat gcccgcggcc
     4801 ttccagatct ccttctcctt ggcatgggtt ccggaattgt cgcctcggga cacgaagagg
     4861 gcattggcgg cgctgatctt tttgaatccc tccgtcgcct tcttggttcc cttgagggcc
     4921 accgaatccg cgggaggacc tacaacaatg aagtcattgt gcatgaccaa gcggcgattg
     4981 acgccattgc cggcctccat gaatttcttc tccgccgcct gggaatggac cagcaggaca
     5041 tccgcctctc ccttactccc catggtcatg gcctggccgg aaccaacggc gatggtcttg
     5101 acgaaatagc cggtcttctt ctcgaagatg ggaatcagaa catccagaag acccgtgtcc
     5161 tgggtgctgg tcgtggtggc caggatgagg ttcttctgtt tcgccggcgc cttgcgggcg
     5221 gcatcggcgc cgccg
//
LOCUS       DAOSMF010000133         5216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_17333_length_5216_cov_24.749080, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000133 DAOSMF010000000
VERSION     DAOSMF010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5216
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17333_length_5216_cov_24.749080"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="PLT63_09715"
     CDS             <1..221
                     /locus_tag="PLT63_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOD99039.1"
                     /translation="DMQMPVMDGYTATAAIRRWEDDLGLQRTPIVALTAYALKEDEEK
                     SRAAGCDGHLTKPIKKLRLIEEIERYAV"
     gene            239..2299
                     /locus_tag="PLT63_09720"
     CDS             239..2299
                     /locus_tag="PLT63_09720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOD99040.1"
                     /translation="MSDETNDNGERRETFYRILAESSFAGIYVVQNGIFRYLNHNAAS
                     YAGYEPEELTGCKANGIVHPDDQEKVMRFARAMLKGLRADPYEFRIVTKQGNIRWIME
                     TVTAIRYEGLPAILGNSMDVTEKKETETALRVSEERYRNIIEHSGVLFYTHAPNHVLT
                     FVSANAEHFFGCTPEEATISWQEFLTDNPVNRHGLEATQRALETGERQPPYELELWKQ
                     NGETIWVEVHEHPVTVGGEIQGIVGTLVDVSLRKWMQRALTESEERYRTLIETTPDLV
                     YTVDRQGVFTYANQRFEEALGIPPADLLGRPFTAIIPPESIPVIVDRFKRGIRGEAIP
                     PYEVALTAQNGQRVIIEFLTSTFLDPLTGWATGRFGIGRNVTERRQAQDALKASEERY
                     RTIIENIGDGYYEVDVNGNLRFANDACRRITGQSLDKIIGANFRVFFFPEDIADIIRM
                     FNATFVTGESKRGMVKRLQRTDGKEQYVEISVSLKRDGKGKPSGFMGILHDVTERRKS
                     EETIHWLAYHDALTGLPNRLLFRDRLVMAIAQAKRNGEQLAVMMMDLDKFKEINDTRG
                     HDVGDAVLCSFTERIKKRLRAGDTFARTGGDEFMIIMPRIRRREDLAMLGKTLVGALA
                     APVTMGAATFKFSVSIGIALYPRDGMDFDALVKNADTAMYLAKGRGGNAYEIFAAPAG
                     EKNA"
     gene            2452..3309
                     /gene="panC"
                     /locus_tag="PLT63_09725"
     CDS             2452..3309
                     /gene="panC"
                     /locus_tag="PLT63_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041584793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOD99041.1"
                     /translation="MKIIETVKEMQAFAESLRNAGKKIAFVPTMGYFHEGHLNLMRIG
                     RKLGDCLVISIYVNPTQFGPNEDLAKYPRDFERDRELAASVGVDVIFYPPNEEMYPPG
                     YQTYVNVEEVTNNLCGLSRPGHFRGVTTVCCKLFNMVKPHVTIFGKKDFQQLTAIKRM
                     VIDLNMDLEVVPMETTREPDGLAMSSRNIYIKLEERESALSLSRSLKMAKEMYERGER
                     EAGVILAAVRKFIEGHPHARIDYVQICDTGTMKDVERLDGEAVIALAVRVGSARLIDN
                     YVFGAPLEI"
     gene            3326..3682
                     /locus_tag="PLT63_09730"
     CDS             3326..3682
                     /locus_tag="PLT63_09730"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HOD99042.1"
                     /translation="MQRFMLKSKLHRAVVTDANLHYEGSITIDEGLLEAADIIPYEKV
                     AIYNVTNGERFTTYAIKGARGSGTICLNGAAAHRASKGDVIIIATYVVAEASELSGWS
                     PKCVLLNEKNQIKKIG"
     gene            3762..4409
                     /locus_tag="PLT63_09735"
     CDS             3762..4409
                     /locus_tag="PLT63_09735"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HOD99043.1"
                     /translation="MSKKIKKIFIAGLAVTIPIGLTLYVLFFLIEVMDDLLRIIPHRL
                     HPDTLLQFHIPGLGIIFVVILIFAAGLMMKSYLGNRIVQMSEAVFHRIPVIRSIYDGT
                     KQVMDRMFVHNNRSFKKVVLVEFPRAGAFTLGFVTGPAHRRIGKSTGRPCTNVFIPTT
                     PNPTSGYLLAIPDEELVEIDMTVEEALTYVVSCGLVQPPSLEMRPGETAAGQKIY"
     gene            4468..>5216
                     /locus_tag="PLT63_09740"
     CDS             4468..>5216
                     /locus_tag="PLT63_09740"
                     /inference="COORDINATES: protein motif:HMM:NF014791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HOD99044.1"
                     /translation="MNIASESVKVGHPDMVCDAIAANIIADVLSEEKKIGMTIDNMPH
                     CGLEVFLGKGLCVVGGEVATRTYVDIEKVIRNTVLSLGYKDSAVGLNGNSMGVLNAMI
                     PQSPDINIGTRADKGKYKEIGAGDQGIIYGFACDETPELLPLPYVLGNRLMRAFEYCG
                     NPVFAPDGKGQVTVQYDEDMRPVRLATILMSNAIDYRHVPDTFRNTIEHVARSIAMET
                     LADYVDAKTEFLFNPTGEWQAVNSCSAADSGV"
BASE COUNT         1184 a         1495 c         1558 g          979 t
ORIGIN      
        1 tggacatgca gatgccggtc atggacggct atacggcgac ggcggccatc cgccgctggg
       61 aggacgatct gggattgcag cggacgccca ttgtggccct caccgcctat gccctgaagg
      121 aggacgagga gaagagccgg gcggcgggct gtgacggcca ccttaccaaa cctataaaga
      181 aactcaggct tattgaggag atagaacgat atgcagttta agggagcagt ggcggtttat
      241 gagtgatgag accaacgaca acggagaaag aagagaaacg ttctaccgga tcctcgcgga
      301 atcctccttt gccggcattt atgtggtgca aaacggcatc ttccgctatc tgaaccacaa
      361 cgccgcttct tatgccggtt atgagccgga ggaattgacg gggtgtaagg cgaacgggat
      421 tgtccaccct gacgatcagg aaaaggtcat gcggttcgcc cgggccatgc tgaaaggatt
      481 gcgggccgat ccctacgagt tcaggattgt taccaagcag ggcaacatcc gctggataat
      541 ggaaaccgtg acggccatcc gctatgaagg acttccggcc atcctgggca attccatgga
      601 cgtgacggag aagaaggaaa cggaaacggc attacgggtt agcgaggaac ggtaccggaa
      661 cattatcgaa cacagcggcg tcctgttcta cacccacgcc ccgaaccacg tcctgacctt
      721 tgtcagcgcc aacgccgaac attttttcgg atgcaccccg gaggaggcga cgatttcctg
      781 gcaggagttt ctcaccgaca atcccgtcaa ccgtcacggc ctggaagcca cgcagcgggc
      841 cctggaaacg ggggagcgac agccgcctta tgagttggag ctgtggaagc aaaacggcga
      901 gacaatctgg gtggaagtgc atgagcatcc ggtgacggtc ggcggcgaga ttcagggaat
      961 tgtaggcacc ctggtggatg tcagcctccg caagtggatg cagcgggcct tgacggaaag
     1021 cgaggagcgg taccggaccc tgatcgaaac caccccggat ctggtatata ccgtagatcg
     1081 gcaaggggtt tttacctacg ccaatcagcg ttttgaagag gcgctgggaa tcccgcccgc
     1141 cgatctcctc ggccggccgt tcaccgccat cattcctccg gagagcatcc ccgtcatcgt
     1201 cgataggttc aaacggggga taaggggaga ggcgataccc ccctatgaag tggcgctgac
     1261 ggcgcagaac gggcagcggg tgatcatcga gtttctcact tccacctttt tggaccccct
     1321 taccggctgg gcgacgggga gattcggcat cggccgcaac gtgacggagc gccgccaggc
     1381 ccaggacgct ctcaaggcca gcgaggaacg ctaccggacc attatcgaga acatcggcga
     1441 tggctattac gaagtcgatg ttaacggcaa cctccggttc gccaacgatg cctgccggag
     1501 aataacggga cagtctctcg ataaaatcat cggggccaat ttccgtgttt ttttctttcc
     1561 ggaggatata gcggacatta tccggatgtt caatgccact tttgtaaccg gtgaatctaa
     1621 aaggggaatg gtaaagcgtc tccaaagaac cgatggcaag gaacagtacg tggagatttc
     1681 cgtatcgctc aaaagggacg gcaagggaaa accgtcgggt tttatgggga tcctccatga
     1741 tgtcacggaa cggcggaaat ccgaggaaac gatccactgg ctggcctatc atgacgccct
     1801 gaccggtttg cccaaccgcc ttctcttccg cgaccgcctg gtcatggcca tcgcacaggc
     1861 caaacgcaac ggcgaacaac tggccgtaat gatgatggat ctggataaat tcaaggagat
     1921 aaacgacacc cgcggccatg acgtgggcga tgccgtcctg tgctctttta ccgaacggat
     1981 aaaaaagaga ctgcgggcgg gggatacctt tgcccgcacc ggcggcgacg agttcatgat
     2041 catcatgccc cggatacgcc gccgcgagga tctggccatg ctgggaaaga ccctggtcgg
     2101 cgccttggcc gcgccggtga cgatgggagc ggcgacgttc aaattcagcg tcagcattgg
     2161 catcgccctt tatcctcggg atggcatgga tttcgacgcc ctggtaaaaa atgccgatac
     2221 ggccatgtac ctggccaagg ggaggggcgg caacgcctac gaaatattcg ccgctccggc
     2281 gggtgaaaaa aacgcttgac gatcggccgc cattctgtta cgcggaaatc caccgcttgg
     2341 atccccgtcg gggaccggga cgggaaggta tgaggataag cagcaaggga caaaactcca
     2401 ggcctcccgt cattgacggc gaggcttttt tcgttggggg aggagatgcc aatgaagatc
     2461 atcgagacgg taaaagagat gcaggcgttt gccgaatcgc tccggaacgc cgggaaaaag
     2521 attgccttcg tgccgaccat gggctacttt cacgaagggc acctgaacct gatgcgcatc
     2581 ggccggaaac tgggggattg tctggtcatc agcatctacg taaaccccac ccagttcggt
     2641 cccaatgagg acctggcgaa atatccccgg gatttcgagc gggaccggga actggccgcc
     2701 tccgtaggcg tcgatgtgat cttctatccc cccaacgagg agatgtatcc tcccggatat
     2761 cagacctatg tgaacgtcga ggaggtgacg aacaatctct gcgggctctc ccggcccggg
     2821 cacttccggg gcgtcacgac ggtctgctgc aagctcttca acatggtcaa accccatgtc
     2881 accatcttcg gcaagaagga cttccagcaa ctgacggcca tcaagcgcat ggtgatcgac
     2941 ctcaacatgg acctggaggt ggtgcccatg gagaccaccc gggagcccga cggcctggcc
     3001 atgagttccc gcaacatcta tatcaagctg gaggagcggg aatcggccct gagcctgagc
     3061 cgttccctga agatggcgaa ggagatgtac gaacgggggg agcgggaggc cggggtcatc
     3121 ctcgcggcgg tgcggaaatt tatcgagggg catcctcacg cccggatcga ctacgtgcag
     3181 atctgcgaca ccggcaccat gaaggatgtg gaaagattgg acggcgaagc cgtgatcgcc
     3241 ctggccgtac gggtggggtc ggcgcggctg atcgataact acgtcttcgg cgcgccgctg
     3301 gagatatgaa cgaggagagg gaacgatgca gaggttcatg ctgaaatcga agcttcatcg
     3361 tgccgtggtc accgatgcca acctccacta cgaggggagc atcacgatcg acgaaggcct
     3421 gctggaggcg gcagatataa tcccctatga aaaggtggcc atctacaatg tcaccaacgg
     3481 ggagcgcttc accacctatg ccatcaaggg ggcccgggga tccgggacga tctgcctcaa
     3541 cggggcggcg gcccaccggg cgtccaaggg ggatgtgatc atcatcgcca cctatgtcgt
     3601 cgccgaggct tcggaactct cgggatggtc gcccaagtgc gtcctcctca acgaaaagaa
     3661 tcagatcaag aagatcggtt gatcatccgg actcaccaag actatgcggg cggggcgccc
     3721 gggcgcccgg cccgttcttt ttcttccgca acaatgactg aatgagcaag aaaatcaaga
     3781 agatattcat tgccggcctg gcggttacca tccccatcgg attgacgctc tacgtccttt
     3841 ttttcctcat cgaggtcatg gacgacctgc tccggatcat tccccaccgc ctccaccccg
     3901 ataccctcct gcagtttcac atcccgggcc tgggaataat cttcgtcgtg atcctgatct
     3961 tcgccgccgg cctcatgatg aagagctatc tgggcaaccg gatcgtacag atgagtgagg
     4021 ccgtatttca tcgcatcccg gtgatccgca gcatttacga cggcaccaag caggtcatgg
     4081 accgcatgtt cgtccataac aaccggagct tcaagaaggt ggtcctggtg gagttccccc
     4141 gggccggcgc cttcaccctc ggctttgtca ccggcccggc ccaccgccgc atcggcaaat
     4201 cgaccggccg cccctgcacg aacgtgttca ttcccaccac ccccaacccc acttcgggct
     4261 acctcctggc gattcccgat gaagaactcg tggagatcga catgacggtg gaggaggccc
     4321 tgacctatgt cgtttcctgc ggcctcgtcc agcccccgtc cctggaaatg agaccggggg
     4381 aaacggccgc cggccaaaaa atatattgat gagttcgaga tggttgtgtt agaaatctcc
     4441 caaaaacgga acaaaggaga tgaagccatg aacattgcct cggagagtgt caaggtcgga
     4501 catccggaca tggtttgcga cgccattgcc gccaatatca ttgccgatgt cctgtcggag
     4561 gaaaaaaaga tcggcatgac catcgacaac atgccccatt gcgggctgga ggtatttctg
     4621 ggcaagggcc tctgcgtggt cggcggcgag gtagccaccc gcacctacgt ggatatcgaa
     4681 aaggtgatcc gcaataccgt cctgtcgctg ggttacaagg attcggcggt gggcctcaac
     4741 ggcaattcca tgggggtcct caacgccatg atcccccagt ctccggacat caatatcggc
     4801 acccgggccg ataagggaaa atacaaggag atcggcgccg gcgaccaggg gatcatctac
     4861 ggttttgcct gtgacgagac cccggagctg ctgcccctgc cttatgtcct gggcaatcgc
     4921 ctcatgcggg ccttcgagta ttgcggcaac cccgtcttcg ctcccgacgg gaagggacag
     4981 gtgaccgtgc aatacgatga agacatgcgg ccggtgcggc tggcgacgat cctcatgtcc
     5041 aacgccatcg attaccgcca tgtccccgat accttccgga acaccatcga acacgtggcc
     5101 cgatccattg ccatggaaac cctggccgac tacgtcgatg ccaagacgga gttcctcttc
     5161 aatcccacgg gggaatggca ggcggtcaat tcgtgcagcg ccgcggattc cggggt
//
LOCUS       DAOSMF010000134         5160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_17568_length_5160_cov_20.663859, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000134 DAOSMF010000000
VERSION     DAOSMF010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5160
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17568_length_5160_cov_20.663859"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..358
                     /locus_tag="PLT63_09745"
     CDS             125..358
                     /locus_tag="PLT63_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99045.1"
                     /translation="MSDREQETLLREMQSRFNKKLREQEISVVEHWHEQLNRILTLKP
                     EGVAALQNQIRKVAKMMENRIIILKRSPLDGRN"
     gene            484..1035
                     /locus_tag="PLT63_09750"
     CDS             484..1035
                     /locus_tag="PLT63_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOD99046.1"
                     /translation="MLQKTPRSGYQFLGSGCESVAEHILRTIYIAYVLCRMDPRADEH
                     KTLKICLFHDLPEARTGDMNYVNKKYVRVDEAKAVKELTETLPFGEDIAALIREFNEK
                     ETREAKIAHDADQLALIIQLKEYGDLGNKYSQEWISFARKRLCTKNGKKLAHDILKTD
                     SSAWWFKDKSDWWINGTRHKEGT"
     gene            1032..1832
                     /locus_tag="PLT63_09755"
     CDS             1032..1832
                     /locus_tag="PLT63_09755"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD99047.1"
                     /translation="MIQQSSFGIRHTWQAFLSISLGMLLLVSLIACTTTAQKRREMAE
                     THLNLGSAYVEAGDYTAALRELLAAEKDAPRDSKIHYYLAIAYFGKGHNELAAAEAER
                     AVDLKKDYPVAYNLLGTIYYAQGRYDKAIEAFNKALADVLYETPALTLYNLGKAYFRL
                     GNFPQALKKYQEAKEKDARRELLPLIEMEAGKVMYAQGNYAEAIIHFKMAVELAPVLV
                     EAHYWLGETYIKQQKNKEARQAYETVVRLSPSSEFGLKAKKILQFMDT"
     gene            1993..2616
                     /locus_tag="PLT63_09760"
     CDS             1993..2616
                     /locus_tag="PLT63_09760"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HOD99048.1"
                     /translation="MASKKQLRTATVKTRKQKKTMKKSVLVFVITALVGFLVFFFLTL
                     FDYVYPPVGGQGAGAKKKEKEEVTVYFSDANERFLAPEKRYVPKEETQANAARELVKT
                     LLDGSRTGLVNTFPEKVEVIGVKIDNGIASVSFNRNLLKNHPGGSAAEMATIYSLTNS
                     LTDNIPEIKKVKILVAGKEIESIKGHIDTRQAFAPNRELIVQAKKEP"
     gene            2628..4706
                     /gene="fusA"
                     /locus_tag="PLT63_09765"
                     /pseudo
     CDS             2628..4706
                     /gene="fusA"
                     /locus_tag="PLT63_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229047.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
     gene            4745..>5160
                     /locus_tag="PLT63_09770"
     CDS             4745..>5160
                     /locus_tag="PLT63_09770"
                     /inference="COORDINATES: protein motif:HMM:NF019500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HOD99049.1"
                     /translation="MIRLTIILVSAGAVYYFEYQQSSSNINNIWDGIWWAIVTMGTVG
                     YGDKYPVTTGGRFVGILLIFTGVGLMSLFTATIASAFVERRMKEDRGLETIKAREHIV
                     ICGWNQHTEKVIQGLTTYGVMGEKTIVLINELSVEE"
BASE COUNT         1361 a         1207 c         1484 g         1108 t
ORIGIN      
        1 atatatgaat gcacgcttag tgtcaaggtg ttttctcaga aaattcgagg ttgcatcaaa
       61 cagtctaatt gatataagac gaacctcgta ttgaagatgg accatcagac cgggaggaag
      121 ggaaatgagt gacagggaac aggaaacgct gctcagggaa atgcaatcca ggttcaacaa
      181 gaaattgcga gaacaggaaa tctcggtggt cgagcactgg catgagcagt tgaaccgcat
      241 cctgacactg aaaccggaag gagtggcggc acttcagaat cagatccgga aggtggccaa
      301 gatgatggaa aacaggatca tcattctcaa acgatcgcca ctggatggca ggaattgaag
      361 gcgtcgtcgc ctgtgcttag ggtatgtaac cgaggatgac cggattgatt gaaaacgagg
      421 ctgactagtt cgctcctttt tttaaccatg aacgataccg caaatttcct ttacgaagtc
      481 ggaatgttac aaaagacccc ccgttcggga taccagtttc tcggctccgg ctgtgaatcc
      541 gtggctgagc atatcctcag gacgatctat atcgcctacg tactgtgccg gatggatccc
      601 agggcggatg aacataaaac cctcaaaatt tgcctcttcc atgatttgcc cgaggcgaga
      661 acgggcgaca tgaactatgt caacaagaag tatgttcgag ttgatgaggc gaaggcggtg
      721 aaggagttga cggagacact gccctttggc gaagacatcg ctgccctgat cagggaattc
      781 aatgaaaagg agaccaggga agcgaaaatc gcccacgatg cggatcaact ggcgcttatc
      841 atccagttga aggaatacgg cgacctggga aacaaataca gtcaggagtg gatttcgttc
      901 gcccgcaagc gcctttgtac caaaaacggc aaaaaactcg cccatgatat tctgaagacc
      961 gattcgtcgg cgtggtggtt caaggataaa agcgactggt ggataaatgg gacgcgccac
     1021 aaggagggga catgatacaa cagagcagct tcggtattcg ccatacgtgg caggcctttc
     1081 tgtcgatctc tctgggtatg ctcctactgg tttccctgat cgcctgcaca acgaccgcgc
     1141 aaaaacgccg ggagatggcg gaaacccact tgaatctcgg cagcgcttac gttgaggccg
     1201 gggattatac cgcggcgttg agagagcttt tggcggcgga gaaagacgct ccgcgcgatt
     1261 ccaagatcca ctattatctg gcgattgcct atttcggcaa ggggcataac gaattggctg
     1321 cggcggaggc ggagcgggcg gtggacctga agaaggacta tcccgtagcc tataatttgc
     1381 ttggcacgat ttattacgcc cagggaagat acgataaggc gattgaagcc tttaacaagg
     1441 ccctagccga tgtcttatac gagacgcctg ccctgactct ttacaatctc ggtaaggcct
     1501 atttccgcct tggtaatttt ccccaagccc tgaaaaaata tcaggaggca aaggagaagg
     1561 atgcccggcg cgaactgcta ccgctgatcg agatggaggc gggcaaggta atgtatgccc
     1621 aggggaatta cgccgaagcg ataatccatt ttaaaatggc ggtggaactg gcaccggtgc
     1681 tcgttgaggc acattactgg ctgggtgaga cctacatcaa acaacaaaaa aacaaggaag
     1741 cgagacaggc ttacgaaacg gtggttcggc tttctcccag ttcggaattt ggtctcaaag
     1801 ccaaaaagat attacaattc atggacacat gaaggtagct ggacaacctg gggactgtat
     1861 ctccggggcg gtaataatgg gcgtatttcg ccaggccact gaagagatgc ggtggttggg
     1921 cgatgtttcc atctttactt gacggcgttg gtaatgtaac ctcacttatc acccaaacat
     1981 caggagcttg tgatggcctc taagaagcaa ttaaggaccg ctacggtcaa aaccaggaag
     2041 cagaaaaaaa cgatgaagaa atccgtccta gtgttcgtga tcacggccct ggtaggattc
     2101 ctggttttct tctttctcac cctcttcgat tatgtctatc cgccggtagg cggccagggt
     2161 gcgggggcca agaaaaagga gaaggaagag gtgaccgtat atttctccga cgccaatgaa
     2221 cgtttcctcg caccggaaaa gaggtatgtc ccaaaggaag agacccaggc aaatgcggcg
     2281 cgggaactgg tgaaaacctt gctggacggt tctcgcaccg gcttggtcaa tacctttccg
     2341 gaaaaagtgg aagtaatagg tgtaaaaata gataacggta tcgcctccgt gagcttcaac
     2401 cgcaatctct tgaaaaatca tcccggcggc agtgccgccg agatggcgac catatattcc
     2461 ctgaccaatt ccctgacgga taatattccg gaaatcaaaa aggtcaaaat cctcgtggcc
     2521 ggaaaggaga tcgaatcaat caagggccat attgataccc gccaggcctt tgcacccaat
     2581 agggaattga tcgtccaggc caaaaaagag ccttaggatt acacgttatg ccacccagat
     2641 caaagcttgc caagataaga aatatcggca ttgtggcgca tattgatgcc ggaaagacca
     2701 cggtaacgga aaggatactc tactatactg gccgttccta taaaatgggt gaagtgcatg
     2761 acggagaagc tgtcatggat tggatgcccc aggagcagga gcggggcatc acgatttcct
     2821 cggccgtgac cacatgcaac tggcatgatt gcgagattca tatcattgat actcccggcc
     2881 atgtggattt caccattgag gtcgaaaggt gcctgcgggt cctcgacggc gccgtggctg
     2941 tattctgcgc ggtgggcggc gtggagcccc aatcggaaac ggtgtggcac caggccgaca
     3001 aatacggcgt cccgaaaatt gccttcatca acaagatgga tcgggtcggg gcggattttt
     3061 tcggcactgt ggcgatgatg aaacagcggt tcaactctgt gcctctgccg atccagatcc
     3121 cctggggaga ggcggatcag tttcaaggca taatcgactt gatcaggcaa aagctgtttg
     3181 tctgggacgg cacctcgctg gggatggcat attcccagtc ggaaatcccg ccggacatga
     3241 aggaatcagc ggaccggcag cgagagaaga tgattgaaac ccttgccgaa catgacgacg
     3301 atcttgcgga tagatatctc gaagggttgg agataagcga agaagatatt gtccgggcca
     3361 tccgcaatgc ggccctgaag ctgtgggtcg tgccggtaat gtgcggttcc gccttgagga
     3421 acaagggtat acagccggtt ctcgatgccg ttttggctta tctgccttct ccggaagatg
     3481 ttcctcccgt ccgtggtgtg aatcccataa ccagagagga agaggttcgc gccagtgatc
     3541 cccagggacc cctggcggcc ctggtattca agataatgca ggatgaagga cgcaaacttt
     3601 cttatctacg catttattcc ggaaagatca aggccggcga cgatctctac aatatctcca
     3661 aagggagaaa ggaaaaagta tccaggcttt tgaagatgca tgccaacaag cgggagcgcc
     3721 tggaggcggc ccaggcgggg gatattgtcg ccgtaatggg tctcaaggag gccaagacag
     3781 gggacactat ctgtgacgag gcccggccgc tgcttttgga atcaatggaa ttctatgagc
     3841 cggtgatcag ccaggccatt gaggcgaaaa cccccgccga tcaggaaaga gtttccctgg
     3901 ccctggtaaa gcttatggaa gaggacccta ccttgcgtct caaatttgat gatgaaacgg
     3961 cgcaaacggt tatctcgggg atgggggaac tccatctgga gattctcata gaccgtttgc
     4021 agagggaatt caatgcccgg gtcaacgtgg gtaaaccgcg ggtagtatat cgcgaaacca
     4081 tccagacgcc ggtggaggtg gagggaaaat ttgaaaggga gatcggcgac cgcagacatt
     4141 acggacatgt actcctccag ttgcgaccac gggaacgggg aatcggaaac ggttatgcga
     4201 accatgtcaa ccccgaggtt ctgccgctgg agttccatac cgcaatcgaa gagggagtgc
     4261 aagaagccat gatgagcggc gtcatagccg gctatccggt ggtggatgtg gaaacgctag
     4321 tgaagagcgc ctctttccgt gatggtgagt cgtcgccgct gggatacaag attgccgcct
     4381 ccatggcagt gcgagaagga tatgaaaggg gtgggtcact gctgctggag cccattatga
     4441 ttgtcgatgt gctcacaccc agtgaattca tgggcgaggt gatcggagac attaacgcgc
     4501 gccggggcga aatactatcc ataaccccaa aaggagcagt cagtgagatc agggcgaagg
     4561 tgcccctcaa agccctcttt ggatattcca cagatctccg ttcagcgacc cagggccggg
     4621 cgacgttctc catgcatttt ttccgttatg acaaggtgtg agggtggtac gaattacctc
     4681 gtcgttccgg caagtccata aggtaatttc gggtttttat caaaagccgg cggtgcaggt
     4741 ggcaatgatc agactgacca tcattttagt ttccgccggg gcagtttatt atttcgaata
     4801 ccaacaatcg agttcaaaca taaacaacat ctgggatggc atttggtggg ccatcgtgac
     4861 catgggaact gtcggttacg gggataagta tccggtaacc acgggaggtc ggtttgtcgg
     4921 catcttactt atctttaccg gtgtgggact tatgtcgctg ttcaccgcta ccatcgcttc
     4981 ggcctttgtt gagagaagaa tgaaggagga caggggattg gaaacgatca aggccaggga
     5041 gcatatcgtc atctgtggat ggaatcagca taccgagaag gtcattcagg ggctgaccac
     5101 ctacggggta atgggagaga aaaccatcgt cctgatcaac gaactgagtg tggaggagat
//
LOCUS       DAOSMF010000135         5093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_17863_length_5093_cov_25.042676, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000135 DAOSMF010000000
VERSION     DAOSMF010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5093
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17863_length_5093_cov_25.042676"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..1602)
                     /locus_tag="PLT63_09775"
     CDS             complement(31..1602)
                     /locus_tag="PLT63_09775"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOD99050.1"
                     /translation="MAKEQGKGVTEEKIRVFEARREALKTMGGEKMIAKQHELGKLTA
                     RERLAMFYDEGTFQEVQLFVKHRSPLFGLDKKQINADGVIVGFGKVNGRTVFAAAQDF
                     TSSGGSLGEMHAKKIWKVMDMALDARKPFVSMNDSGGARIQEGVPALDGYGGIFYRNT
                     IGSGYIPQITAIMGPTAGGAVYSPALTDWVFMVKKSSYMYITGPDVIKAVIGEEVTHD
                     DLGGAVAHATKSGVCHFATENDEDCINKVKTLLSYLPDSCHSSLPIAPCTDSPDRDCP
                     ELDKIIPDRATRGYDMKQVVMAVADNNEIFQPHELWAKNILVAFIRIMGQPVGVIANN
                     PKFGAGVLDTTASDKASRFIRFCDAFNIPLLTFSDVPGYMPGTAQEWAGIISHGAKLL
                     HAYSEATVPKITVVTRKDYGGAYIGMCSKYLGADYVMAWPSAEIAVMGAEGACNIIYR
                     REINEAADPAARRSELVAAYEEQFNNPYFAASMGIIEEIIAPRETRKRVAVLLDALKD
                     KSQVRPPRKHNNIPL"
     gene            1867..3054
                     /locus_tag="PLT63_09780"
     CDS             1867..3054
                     /locus_tag="PLT63_09780"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006863552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOD99051.1"
                     /translation="MKFIDQFSLKDKKILFRFDYNVPLDGSQHITDDTRIRATLPTLN
                     YALDEGAQIIIASHLGRPKGKVKPEFSLAPVARRLSRLIQKEVRLAPDCVGPETTALV
                     AAMKPGDVILLENLRFHPEEEKNDEDFARQLAAYADIYIDDAFGNAHRAHASNVAITK
                     FVKECGGGFLMKKEVNYFQKCLEKPARPFVAIIGGSKVSGKLEALVNLIRKVDKMIIG
                     GGMAFTFLKALGYEVGKSLVEEDLMPKALEVVQTARETGVKFYLPVDCVMAEEMSAQA
                     ETKIVPIQEMNSHWMGLDIGPATITLFTEALANAKTIVWNGPMGVFEIDAFGRGTAAM
                     VHSIANSYAMTIVGGGDTDVAVHKTGESDKITYISTGGGASLELLEGKVLPGIAALDA
                     CGG"
     gene            3091..3450
                     /locus_tag="PLT63_09785"
     CDS             3091..3450
                     /locus_tag="PLT63_09785"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOD99052.1"
                     /translation="MTQSTQGNTNSDKCLTIIGRGCLRFLCLINLGEIIYLTKRRFGD
                     EKKIDILSRIHQIGFNLISISASLVFQAAEFKAQYPLSYADCFALACAVNQSATLVTG
                     DPEFRVVSHLIDINWIG"
     gene            3479..4210
                     /gene="truA"
                     /locus_tag="PLT63_09790"
     CDS             3479..4210
                     /gene="truA"
                     /locus_tag="PLT63_09790"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOD99053.1"
                     /translation="MTLEYDGSGYHGWQRQKNGRSIQEVLEERIAVITGEPIKVIGSG
                     RTDAGVHALGQVAHFQTACGIPAGNLLKGINSLLPADIVVKSLDDAPPGFHARIDARR
                     KTYLYRIYNGATPTALYRRSVWQVYGPLDTVAMRAAAAHLLGRHDFTSFCTVHTDAVS
                     FTREIYRLEISAVACDPGLFLEIEVEADGFLRYMVRTIVGTLVEIGRGKRPAAEMGEI
                     LRGRNRDRAGMTAPPQGLFLKEVRY"
     gene            4260..>5093
                     /gene="rfbD"
                     /locus_tag="PLT63_09795"
     CDS             4260..>5093
                     /gene="rfbD"
                     /locus_tag="PLT63_09795"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HOD99054.1"
                     /translation="MQILILGHKGMLGTALMQRLGPFHAVVGKDVEDFDLTSPDSCRE
                     LIAAEKPEVVINAAAYTNVDGAESDAERCFAVNAAGVQNITAACRGRRIRIVHFSTDY
                     VFDGTAAAPYREEDPPAPRGVYAASKAAGEKYLQEGSDDYLLIRTAWLYGPTGKNFVK
                     TILEKALHNPTLRVVDDQVGCPTYSRDLAGAVAVLLEQGHRGIFHVTNRGSCSWFEFT
                     RKILAGAGFNRVSVEPVKTADLGLAAPRPAYSVLTSAKFTAATGKTLRFWPLALEDFL
                     AH"
BASE COUNT          988 a         1541 c         1578 g          986 t
ORIGIN      
        1 taaaaacgct aaaaaacaag accccatccc ttacaggggg atgttgttgt gcttccgggg
       61 cggccggacc tgcgacttgt ccttcagggc gtcgagaagg acggcgacgc gcttccgggt
      121 ctcccggggg gcgatgatct cttcgatgat ccccatggag gcggcgaagt agggattgtt
      181 gaactgctcc tcataggccg cgaccagctc cgaccgcctg gcggcgggat cggcggcctc
      241 gttgatctcc cggcgataga tgatgttgca cgccccttcc gcgcccatga cggcgatttc
      301 cgccgacggc caggccatga cgtagtccgc gcccaggtac ttgctgcaca tgccgatgta
      361 ggcgccgccg taatccttcc gggtgacgac ggtgatcttg ggcaccgtgg cttcggaata
      421 ggcgtggagg agtttcgctc cgtggctgat gatgccggcc cactcctggg cggttccggg
      481 catgtagccg gggacgtcgg agaaggtgag cagggggatg ttgaaggcgt cgcagaagcg
      541 gatgaaccgc gaggccttgt cggaggcggt ggtgtcgaga acgccggcgc cgaatttggg
      601 gttgttggcg atgacgccca cgggctgccc catgatgcgg atgaaggcca caaggatgtt
      661 cttcgcccac agctcgtggg gctggaagat ctcgttgttg tccgccacgg ccatgacgac
      721 ctgcttcatg tcgtagcccc gggtggcccg gtcggggatg atcttgtcca gctcggggca
      781 atcccggtcg ggggaatcgg tgcagggcgc gatgggcagg gaactgtggc aactgtcggg
      841 cagataggac agcagggtct tcaccttgtt gatgcagtcc tcgtcgtttt ccgtggcgaa
      901 gtggcagacg ccgcttttcg tggcgtgggc cacggcaccg cccagatcgt catgggtgac
      961 ctcctcaccg atgaccgcct tgatgacgtc cggtccggtg atgtacatgt aggaactctt
     1021 cttcaccata aagacccagt cggtaagggc cggggaatag acggcgccgc cggcggtggg
     1081 tcccatgatg gcggtgatct gggggatgta ccccgagccg atggtgttgc ggtagaagat
     1141 gccgccgtag ccgtccaggg ccgggacgcc ttcctggatc cgggcgccgc ccgaatcgtt
     1201 catggacacg aagggcttcc gggcgtccag ggccatgtcc atgaccttcc agatcttctt
     1261 ggcgtgcatc tcgcccaggc tcccgccgga gctggtgaag tcctgggccg ccgcaaagac
     1321 ggtccggccg ttcaccttgc cgaaaccgac gatgacaccg tcggcgttga tctgcttctt
     1381 gtccaggccg aagagggggg aacggtgctt cacgaacaac tgcacctcct gaaatgtccc
     1441 ctcgtcataa aacatcgcca gacgttcccg ggccgtaagc ttgccgagtt catgctgctt
     1501 ggcgatcatc ttttcgccgc ccatggtctt cagggcttcc ctgcgggcct cgaatacacg
     1561 gatcttttct tccgtcactc cttttccttg ttctttcgcc ataaacctac tctctcctta
     1621 tctcctgatt ttattctcgt acgtgatgta catggaattc gattcgacct tgaccggagg
     1681 ggccttctca tgtatagaag cttgccgcaa ggtttcctat gggattgcta atgcggcaat
     1741 acctcgccgg agaaaaatcc ggcctccctt gcctcgttaa aatcgtaaca tattgtaatt
     1801 taaaaacttt ttaccttttt cgggacgcat catagcctgt ttgtcaaaaa tatgcaagga
     1861 ggatcaatga aatttatcga tcagttctca ctcaaagaca aaaaaatcct gttccgtttc
     1921 gactacaacg ttcccctgga cggctcccag cacatcaccg acgacacccg gatccgggcg
     1981 accctgccga cactgaatta cgccctggac gagggggcgc agatcatcat cgcctcccac
     2041 ctgggacgcc ccaaaggaaa ggtgaagccg gagttcagcc tggcccccgt ggctcggcgc
     2101 ctctcccggc tcatccagaa ggaggtgcgc ctggcaccgg actgtgtcgg gccggaaact
     2161 acggctctgg tggcggccat gaaacccggg gatgtaatcc ttctggaaaa cctccgtttc
     2221 catccggagg aagagaagaa cgatgaggac ttcgcccggc agttggcggc ttacgccgat
     2281 atctacatcg acgacgcctt cggcaacgcc caccgggccc atgcctccaa tgtggcgatc
     2341 acgaaattcg tcaaggaatg cgggggcggc ttccttatga aaaaagaggt caattacttc
     2401 cagaaatgtc tggaaaaacc ggcccggccc ttcgtggcca ttatcggggg ctccaaggtg
     2461 tccggcaagc tggaggccct ggtcaatctc atccggaagg tggacaagat gatcatcggc
     2521 ggcggcatgg cctttacctt cctcaaggcc ctgggctacg aggtgggaaa atctctcgtg
     2581 gaagaagatc tgatgcccaa ggcgctggag gttgtgcaga cagcccggga aacgggggtc
     2641 aagttctatc tccccgtaga ctgtgtcatg gcggaggaga tgagcgccca ggcggagacg
     2701 aagatcgtcc ccatccagga gatgaacagc cattggatgg gcctggatat cggtccggcc
     2761 accatcaccc tgttcaccga ggccctggcc aacgccaaga ccatcgtctg gaacggaccc
     2821 atgggggtct tcgagatcga cgccttcggg cggggcacgg cggccatggt gcacagcatc
     2881 gccaattcct acgccatgac catcgtcggc ggcggcgaca ccgacgtggc ggtccacaag
     2941 acgggggaaa gcgacaagat cacctacatt tccaccggcg gcggggcctc cctggaactc
     3001 ctggagggca aggtcctccc cggcatcgcc gccctggacg cctgcggcgg atgagagccg
     3061 ataaccgatc gccccggagc agcaaactgg ttgacacaat caacacaagg taatacgaat
     3121 agcgacaagt gtttaaccat tatcgggagg ggttgtttgc ggtttctgtg tctgataaat
     3181 cttggcgaga tcatttattt gactaaaagg cgtttcggtg atgaaaagaa gatcgacatt
     3241 ctaagtcgta tccatcagat cgggtttaac ctgatttcca tttccgcttc cctggttttc
     3301 caagcagcgg aattcaaggc gcaatatccc ctttcttatg cggactgctt cgcgctggcg
     3361 tgcgccgtca accagtcggc aacgctcgtg accggcgatc ccgagttccg ggtggtctcg
     3421 catttgatcg acatcaactg gattggttga aatcggcagc ggcatgcgga cgatccggat
     3481 gaccctggaa tacgacggga gcggctacca cggctggcag cggcagaaaa acggccggtc
     3541 catccaggag gtgctggagg agcggatcgc cgtcatcacc ggggagccga tcaaggtgat
     3601 cggctccgga cggaccgacg ccggggtcca cgccctgggt caggtggccc acttccaaac
     3661 cgcctgcggg atccccgccg gcaacctctt gaaggggatc aacagcctcc tgcccgccga
     3721 tatcgtcgta aagtccctgg acgacgcccc gccgggattc cacgcccgga tcgacgcccg
     3781 gcggaaaacc tatctgtatc ggatctacaa cggggcgacc ccgacggccc tttaccgccg
     3841 gtccgtctgg caggtttacg ggcctctgga tacggtggcg atgcgggcgg cggcggccca
     3901 ccttctgggc cggcatgatt tcacctcctt ctgcaccgtt cacaccgacg cggtctcctt
     3961 tacccgggag atctaccggc tggagatctc cgccgtcgcg tgcgatcccg gacttttcct
     4021 ggaaatagag gtggaggccg acgggttttt gcggtatatg gtgaggacca tcgtgggcac
     4081 cctggtggaa atcggccggg gcaaacgccc ggcggcggaa atgggggaga tcctccgggg
     4141 ccgcaaccgg gaccgggcgg gaatgacggc gccgccccag gggctgtttc tcaaggaagt
     4201 ccgctactga cgacgccgcg ggaagacggc ggatcatatc cggaagggat taaatcttca
     4261 tgcagatact tatcctgggt cacaagggaa tgctcgggac cgcgctgatg cagcggctgg
     4321 gaccgtttca cgccgtcgtc ggcaaggacg tcgaagattt cgacctcacc tcccccgatt
     4381 cctgccggga gctcatcgcc gccgaaaaac ccgaggtggt gatcaatgcc gccgcctaca
     4441 ccaatgtgga cggggcggaa agcgacgcgg agcgctgctt tgccgtcaac gccgcggggg
     4501 tgcaaaacat caccgccgcc tgccggggcc gccggatccg catcgtccat ttcagcaccg
     4561 attacgtctt cgacgggacg gcggccgccc cctaccggga ggaagatccg ccggcgcccc
     4621 ggggcgttta cgccgcctcc aaggccgcgg gggaaaaata cctccaggag gggagcgacg
     4681 attatctcct catccgcacc gcctggctct acggaccgac gggcaagaac ttcgtcaaga
     4741 ccattctgga gaaggccctg cacaacccga ccctgcgggt ggtggacgat caggtgggct
     4801 gccccaccta cagccgggac cttgccgggg ccgtggcggt cctcctggaa cagggacacc
     4861 ggggaatctt ccatgtgacc aaccggggct catgcagttg gttcgagttc acccggaaga
     4921 tcctcgccgg cgccggcttc aaccgggtaa gcgtggaacc cgtcaagacc gcggatttgg
     4981 gcctggcggc accgcggccg gcctacagcg tcctgaccag cgccaaattc accgccgcca
     5041 ccgggaagac cctgcgtttc tggccgctgg ccctggagga tttcctcgcc cac
//
LOCUS       DAOSMF010000136         4987 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_18349_length_4987_cov_23.250811, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000136 DAOSMF010000000
VERSION     DAOSMF010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4987
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18349_length_4987_cov_23.250811"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="PLT63_09800"
     CDS             complement(<1..792)
                     /locus_tag="PLT63_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99055.1"
                     /translation="MRASDIVKGRMKSFGNGNGAYGNNHTPPPKEKIVSEETGSLRLS
                     DIDILNFKSPARKAAIATPAPVMPVTVAPGNDKDPANLERQETPAVAGAAPPEGSEQA
                     LRSDVSERTLGPDGSGRTLAPDGASALPAHEGAGIRKIYLAAKEYMAMVRRSIQAGKS
                     FPLEQALVYVNNILNCSVDVTSQIYQLTVDYEREDDYYLSSPVNVMVYGLKMAQGMGY
                     EKTGLIELGLATLLHDVGMFKIPDAVLNKEGKLTEEEMDMIRRHPE"
     gene            1157..2377
                     /locus_tag="PLT63_09805"
     CDS             1157..2377
                     /locus_tag="PLT63_09805"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HOD99056.1"
                     /translation="MEGKIRRIWSAFMIALLACSIAACFPFLEDTGTPVKEYEAKKTT
                     PQDEARLQKEHQERREELARLSRVKSNSVFTEKRGVADYIVGPGDVLTVNFWTIAVAG
                     AQTFEGFKQITYNVEVRPDGKISYMYGDDIPVSGKTVYEIQELLRADLKKYFRTPRLE
                     VLIKEYKSKTATLFGQINVLPTGTSGPGRYPLTGKMTMIDLISKAGGAITGRSDYSSL
                     PGVAAAGQQNVANADLKNVEFVRKGKKYTVNLYSAMFYGDETQNPVIDDGDIITVPAL
                     PFFADRVYVMGQVASVGIYRLQDAPDLLAALALAGSVTATAVKSDIKVIREFRERGGK
                     PIILSANYNDILKRGDLSQNIKLQSGDVVYVPRMFIGDINEFIANTTPLLNYLLIPAS
                     YRDKYVKDQNFMKW"
     gene            2397..>4987
                     /locus_tag="PLT63_09810"
     CDS             2397..>4987
                     /locus_tag="PLT63_09810"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HOD99057.1"
                     /translation="MLKYDLNLREYWRIIKRRKFIILFTCVIMGVFSLVSAILNKPVP
                     IYRTSAAVKVEKTQSLMGFNAPSYGGANLETQTIMIKSYYILELTAKKMGMIPETLSS
                     EDVRNNPKYIGIILDLKSKVKAEQEGGSDLINIEITANDAKFATRFANALAEVYQRQH
                     TLELNRRTIEGKKFVEGQFAAAKEKVTKSEEAIKKFREDNKWTSVDSESTYTLNEVKR
                     LKSQYNQDQIVLQKLHLAERSLRDAESQPLTSKSSFYFEEASPPYKALNDKLVGYMVD
                     RDTLLLTYTDNFPQIQAIRSQVNDAIAGMRTQLRLQKNNLINNQQILKREIAALEEQF
                     KRLPEKGLELAKLEREMAINREIYTLLEKKYQEILIAEAEKIEEVKIVKPALEPGGPI
                     NPPKIGNNAAIGTLMGLILGVVFAFLIETFDTSIGAIEEVEDFLGVHVLGVIPFVSPE
                     EVRLLLSDDQGRLTVDESKLPRYTRMACHFVPSSTLSESYKAFRTSLNFACMENNYKT
                     LVLTSSSPSEGKTSTIVNLALTMAQTGHKVLLIDGDLRRPVIAGLFGIEQTPGLTDVI
                     MGNYEWRNVVKNISDYMMGHMTVEEVMLTPGLDNLNIITSGTHAPNPAEIIGSKSIAE
                     FMRQVRAEYDMVLVDAPPVLAATDAALWSSQVDATVLVYQVGKIARGALKRAKMQLDA
                     IKTNIIGVVLNGIRAEISTDFTYHDYYYYHYRYGSGKKKQRPWYKEILEKISFDRIVN
                     YGLTYKKRYQRHRKKKDSGVENKSGPETGAAAAAAEGSETSVRLPTEAPAPPNGRNGT
                     VKEAEAVSRRQRFLPVIRIIVTVLAILCLVLGILYQGGFLKAFLPGGKDIPAPKSSLR
                     PMTNSVVV"
BASE COUNT         1164 a         1487 c         1324 g         1012 t
ORIGIN      
        1 ttccggatgt ctccggatca tgtccatctc ctcttccgtc agctttccct ccttgttgag
       61 gacggcgtca gggattttga acattcccac gtcgtgtagg agcgtcgcca gtccgagttc
      121 gatgaggccg gtcttctcgt agcccatgcc ctgggccatc ttcaatccgt aaaccatgac
      181 gttgaccggg gaggagagat aataatcgtc ttcccgttca taatcgacgg tcaactggta
      241 gatctgggaa gtgacatcca ccgaacaatt caggatattg ttgacataaa ccagcgcctg
      301 ctccaggggg aagctcttgc cggcctgaat gctccggcgt accatggcca tgtattcttt
      361 tgccgccagg tatatctttc taatgcccgc tccttcatga gcgggcaatg ccgatgcgcc
      421 gtccggcgcc aatgtccggc cggagccgtc cggacctaaa gtccgttcgg aaacatccga
      481 cctcaacgcc tgttcggaac cctctggcgg cgccgctccg gcgaccgccg gtgtttcttg
      541 ccgttccaaa ttggcaggat ctttgtcgtt tcccggggca actgtcacgg gcatgaccgg
      601 cgccggcgtc gcaattgcag cttttctcgc tggggatttg aagttcagga tatcgatgtc
      661 gctcaatcgc aaggaacccg tttcttcgct gacaatcttt tcctttggcg gcggcgtatg
      721 attgttgccg taggcgccgt tcccatttcc aaacgatttc attctaccct tgacaatatc
      781 cgaggcacgc atatctatat ttccttaaat attaataagt tgacatccaa tgaaaacttc
      841 cgcgcatccc ctgccggggc attcgtttaa acgcattttt tacatcagac cctaacaatt
      901 tgggtgcgta caactatttt tatcaataat caatagttag tttgcttttc ggggatattt
      961 tatttccttg gcactgtgtt caagggcatt caaagtctgg ccccggccgt attgaatttg
     1021 ttgtatttac gatgaattca tgaatattgg ggcgccggcg ccgattatat cccttttcag
     1081 attataatta tacttgactt taatgcgttt ttattgaaga attcgccaaa tcggcaaagt
     1141 cttccccgga ggtcaaatgg agggtaaaat caggcggata tggtcggcgt tcatgatcgc
     1201 cctgcttgca tgttccatcg cggcctgttt ccctttcctg gaagacaccg gcacccccgt
     1261 caaggaatac gaggcaaaga aaacaacccc ccaggatgag gcccgtctcc agaaggagca
     1321 tcaggaacgc cgcgaggaac tggcccggct ctcccgggta aagagcaaca gcgtctttac
     1381 ggagaaacgg ggcgtggcgg actacatcgt cggtcccggc gacgtcctca ccgtcaattt
     1441 ctggacaatc gccgtcgccg gcgcccagac cttcgaagga ttcaagcaga tcacctacaa
     1501 cgtcgaagtc cggccggacg gcaagatctc ttacatgtac ggcgacgaca tcccggtaag
     1561 cggcaagacg gtttatgaga tccaggaact gctccgcgcc gatttgaaaa aatatttccg
     1621 cactccccgc ctcgaggtcc tgatcaagga atacaagagc aagaccgcca ccctcttcgg
     1681 ccagatcaac gtcctgccca cgggcacctc cggtcccggc cggtatcccc ttaccggcaa
     1741 gatgacgatg atcgacctca tctccaaggc cggcggggcc atcaccggcc ggagcgacta
     1801 ctcgtccctg ccgggcgtgg cggcggcggg ccagcagaac gtcgccaacg cggatctcaa
     1861 aaacgtggaa ttcgtgcgca aaggcaaaaa atacaccgtc aacctctaca gcgccatgtt
     1921 ttacggcgat gagacacaaa acccggtaat cgacgacggc gacatcatca ctgtcccggc
     1981 cctgcccttc ttcgccgacc gcgtctatgt aatgggacag gtcgccagcg ttggcatcta
     2041 ccgcctccag gacgccccgg atctccttgc cgccctggcc ctggccggca gcgtcaccgc
     2101 gacggcggta aagtcggaca tcaaggtcat ccgggaattc cgggaacggg ggggcaagcc
     2161 gatcatcctt tccgccaatt acaacgacat cctcaagcgg ggcgacctgt cccagaacat
     2221 aaagctccag agcggcgacg tggtctatgt cccccggatg ttcatcgggg atatcaacga
     2281 attcatcgcc aataccacgc cgctcctcaa ttatctgctg atcccggcct cgtatcgtga
     2341 caaatatgta aaggatcaga atttcatgaa atggtaaccc cgggagcagc cggcgcatgc
     2401 tgaaatacga cctcaatctt cgtgaatact ggcggatcat caagcgccgg aaattcatca
     2461 tcctgtttac ctgcgtgatc atgggggttt tcagcctcgt ctccgcgatc ctcaataaac
     2521 ccgtccccat ctacagaacc tccgccgccg tcaaggtgga aaagactcag tctctgatgg
     2581 gttttaacgc cccgagctac gggggggcca atctggaaac ccagaccatc atgatcaaaa
     2641 gttattatat cctggagttg acggccaaaa agatgggcat gattccggaa accctttctt
     2701 ccgaagacgt ccggaacaat ccgaaataca tcggcatcat tctcgatctc aaaagcaagg
     2761 tcaaggcgga gcaggaaggc gggagcgacc tgatcaatat cgagatcacc gccaacgatg
     2821 ccaaattcgc cacccgtttc gccaacgccc tggcggaggt ctatcagcgg cagcacaccc
     2881 tggagctgaa ccgccggacc atcgagggca agaaattcgt ggagggccag ttcgccgccg
     2941 ccaaagaaaa ggtgaccaaa tctgaggagg cgatcaagaa attccgggag gacaacaaat
     3001 ggacctccgt ggattccgaa tccacctaca ccctgaacga ggtgaagcgc ctcaagtccc
     3061 agtacaatca agatcaaatc gtcctgcaaa agctccatct cgccgaacga tcgttgcgag
     3121 acgcggagtc ccaacccctg acctcgaaaa gcagcttcta tttcgaggag gcatccccgc
     3181 cctacaaggc cctcaacgac aaactcgtgg gttatatggt ggatcgcgac acgctgctgc
     3241 tcacctacac ggacaatttt ccccagattc aggccatccg cagtcaggtc aatgacgcca
     3301 tcgccgggat gcgcacccag ttgcgcctcc agaaaaacaa tctgatcaac aaccagcaga
     3361 tcctcaaacg cgagatcgcc gctctggagg aacagttcaa acgactgccg gaaaagggcc
     3421 tggagctggc taaactggaa cgggagatgg ccatcaaccg ggaaatttac accctgctgg
     3481 aaaagaaata tcaggaaatc ctcatcgccg aggcggaaaa gatcgaggag gtaaagatcg
     3541 tcaagccggc cctggaaccc ggagggccga tcaacccgcc gaagatcggc aacaacgccg
     3601 ccatcgggac gctcatgggc ctcatcctcg gggtggtctt cgcctttctc atcgagacct
     3661 tcgatacctc catcggggcc atcgaagagg tggaggattt cctgggcgtt catgtcctgg
     3721 gggtcattcc ctttgtcagc ccggaggagg ttcgcctgct cctcagcgat gaccagggac
     3781 ggctcacggt ggatgaaagc aagctgcccc gttatacccg gatggcatgc cacttcgtcc
     3841 cctcgtccac cctctcggag agctacaagg ccttccggac cagcctcaac ttcgcctgca
     3901 tggagaataa ctacaagacc ctggtcttga ccagttcctc ccccagcgaa ggcaagacct
     3961 cgacgatcgt gaatctggcc ctgaccatgg cccagacggg ccacaaggtc ctcctcatcg
     4021 acggtgacct gcgccgcccg gtgatcgccg gcctctttgg gatcgagcag acgccgggac
     4081 tgacggacgt gatcatgggc aattatgaat ggcggaacgt ggtcaagaat atctccgact
     4141 acatgatggg acatatgacc gtggaagagg tgatgctcac ccccggtctg gacaatctca
     4201 acatcatcac cagcggcacc catgccccca atccggccga aatcatcggt tccaagagca
     4261 tcgccgagtt catgcgccag gtgcgggcgg agtacgacat ggtgctggtg gacgcccctc
     4321 ccgtgctggc cgccacggat gcggccctgt ggagttccca ggtcgatgcc accgtcctgg
     4381 tctatcaggt cggcaagatc gcccgggggg cgctgaaacg ggccaagatg cagttggatg
     4441 ccatcaagac caacatcatc ggggtggtcc tgaacggtat ccgggccgag atcagcaccg
     4501 acttcacata tcacgactac tactattacc actatcgcta cggcagcggc aaaaagaagc
     4561 aacggccctg gtacaaggag atcctggaga agatcagctt cgaccggatc gtgaattacg
     4621 gcttgaccta taaaaagcgc tatcagcggc accgaaaaaa gaaggatagc ggcgtggaga
     4681 acaaatcagg gccggagacc ggagcggccg ccgcggcggc ggaggggtcg gaaacttcgg
     4741 tacggcttcc gaccgaggcc ccggcgcccc caaatggaag gaacggcacc gtaaaagagg
     4801 cagaggcggt cagtaggcgg caaagattcc tgccggtgat aaggatcatc gtcacggtcc
     4861 tggcgatcct ctgtctcgtg ttgggcatcc tgtaccaggg aggcttcctg aaggcctttc
     4921 ttcccggcgg caaagacatt cccgcgccca agtcttcgct gcgccccatg acgaattccg
     4981 ttgtcgt
//
LOCUS       DAOSMF010000137         4947 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_18568_length_4947_cov_23.190106, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000137 DAOSMF010000000
VERSION     DAOSMF010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4947
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18568_length_4947_cov_23.190106"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="PLT63_09815"
     CDS             complement(<1..707)
                     /locus_tag="PLT63_09815"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOD99058.1"
                     /translation="MLEIKGSVAVITGGGGGIGLEIAKYWVRHGGRVVIADIAQSLLE
                     GAEKQLRELGGEVATVVCDVTKEEDNARLADTAIERFGRINLVAPFAGIIKDGLMVST
                     SKETGKVNRKMSLDQFRAVIDINLTGVFLTVRECAERMINNNCRGLICLVSSTGSLGT
                     AGQINYSSSKAAMSVFPKVITAEFLRRGLSGQIRCVAVAPGYVGTAMVRRMDQKALEK
                     ILADVPIGRLIEPEEVAS"
     gene            complement(1021..2370)
                     /locus_tag="PLT63_09820"
     CDS             complement(1021..2370)
                     /locus_tag="PLT63_09820"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HOD99059.1"
                     /translation="MTEHKRPKWADWADHIRALDDPAKNFEKPEALDDLTVIDLSSRS
                     MAGPYCTSLLAELGAETIRIEPPGGDLVRSYSPNGVMHRGTGMAYLQEGRNKYHVTLN
                     LETEAGRDMLRSLVGQADILIETFPAGKMDNWGIGYEELAKLNPRLIYASITGYGQFG
                     PESGRNQYDYDNVAQARSGVQYATGEVLPEGKTIAEVPYATSTKAGPWIAWAVSGTFG
                     ALGILAAVYHREMTGEGQAIDISTPEAYERLNDYMLHWYADQGVINERFGNLDTCLWL
                     YGFYPTKDGGVFLGGLRLEMWKAFVDIIGKYDEWGAEQWTTLAPFMKKDWQLKYQAMI
                     NPETEQYTSQELTMKSIEYAKRGRLAPITPVVAEIVPPWNTMHDEVWRDRGVFTPVQD
                     PVYGSIICAQAQWKSTETPPRTKWVCRPVGYDNGYVYLKYLGYGPAKLRELTEQGII"
     gene            complement(2376..3704)
                     /locus_tag="PLT63_09825"
     CDS             complement(2376..3704)
                     /locus_tag="PLT63_09825"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HOD99060.1"
                     /translation="MPDVFKDYVKKKFSRPGPLKGVRVLEVCTLLFGPSGPSFLAEMG
                     AEVIKIELPPVGDVTRSLNPFGWFYKEQSPMFMHINPNKYYMALDLHIDLGQQIFKEL
                     AAKADIIEFNLRPGVTRRWNIGYEQIKEVNPGIIYIEKNGFGQWGLYAEQDRPSNDGA
                     SQALSGYAWMSSFPKRPPLKQAIWICDYYGGLMGEVAILAALHHRRKTGRGQFIEMSQ
                     TENIMRVMNWVWPYQQLTGKGVEAAGNRDQCICPADTFLCADDRLAALAAPAPEEFLG
                     LCRAMGREELASDPRFQEHSVRLQDENTLEILKIIAAWARTKTADEIEALGKEFGFSA
                     TRLHNAKDMNEDPHRRQRGFVKEIDDPLYGYYVEHEFPVMMSKTPPKIKWTVRPVGFD
                     NEFIMAKILGRNQAQIDELYLHGVLGKWKDMQGRRPPSDWDGQAGAIIRR"
     gene            4033..4635
                     /locus_tag="PLT63_09830"
     CDS             4033..4635
                     /locus_tag="PLT63_09830"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOD99061.1"
                     /translation="MTAYLDKNRILDAARVVFARHGYRRATLADIVHPLGVTKTAIYH
                     HFSGGKEEIFHALVLREENSVLTDMESAVAAEKEPPAKLRALILAKLTHFQQLRELLM
                     VPRDVGEEVAQLYAGHETSFRASERAMIAAILKEGQRTGIFRPIDTGHLAKNVQTILN
                     RLELPLIYEDTSDHMEHEVDDLLDILFHGIMTAWGENHIR"
     gene            4658..>4947
                     /locus_tag="PLT63_09835"
     CDS             4658..>4947
                     /locus_tag="PLT63_09835"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOD99062.1"
                     /translation="MTEIEKPSDAAAKISKWRRAGRWVMILILAAAMLYGGRWLYWRI
                     GHVTTNAAYIKADMANVVPEIPGKILSIAVKEGQTVRAGQVLLHIDPEQMDR"
BASE COUNT         1012 a         1471 c         1367 g         1097 t
ORIGIN      
        1 gaggccacct cttccggttc gatcagacgg ccgatgggga cgtccgccag gatcttctcc
       61 agggccttct ggtccatccg ccgcaccatg gcggtgccca catagccggg ggccacggcg
      121 acacagcgga tttgtccgga caggccgcgc cggagaaact ccgcggtaat gaccttgggg
      181 aaaacggaca tggccgcctt cgaggaggag taatttatct gccccgccgt ccccagggaa
      241 cccgtagatg acaccaggca gatcaatccc cggcagttgt tgtttatcat ccgttcggca
      301 cattcccgga cggtgagaaa gacccccgta agattgatgt cgatgaccgc ccggaactga
      361 tcgaggctca tcttccggtt caccttgccg gtttccttac tcgtggaaac catgagtccg
      421 tccttgatga tccccgcgaa gggcgccacc agattgatcc ggccaaaacg ctcgatggcc
      481 gtatccgcca gccgggcgtt gtcctcttcc ttcgtcacgt cgcagaccac cgtggcaacc
      541 tcgccaccca gctcccgcaa ctgcttctcc gctccttcca gtaatgactg ggcgatatcg
      601 gcgatgacca cccggccgcc gtgacgcacc cagtatttcg ctatttccag cccgatacca
      661 cctccgccgc ccgtgatgac ggctaccgat cccttgatct ccagcatgct gttctccttt
      721 cccgagcgtt aacaaccagc cgtttccccc cttgccgggg aggattatgg ctggtttcct
      781 gttaccggaa aaatgattac ctggtaataa gtcattcaaa tcaacaccgg tgcaataagt
      841 cgttgagttc ctcccccggc aaggaagtca aaaaacatgg ggaggggatc gcctcccctc
      901 cccatgctgc taatggcaat tctgatgact cctgacgttc gttttagagg caattgccac
      961 cataaatcga attttacaaa tattatcttc cttattgtcc gcagccggca tcaaggtccg
     1021 tcagataatc ccctgttccg tcaattctcg gagttttgcc ggtccgtagc ccagatattt
     1081 caaatacacg taaccattgt cgtagccgac cgggcggcag acccactttg tccgcggcgg
     1141 cgtttccgtg gacttccact gggcctgggc gcaaatgatc gagccataga cggggtcctg
     1201 cacgggggtg aagacaccgc ggtcccgcca gacctcgtcg tgcatggtat tccagggcgg
     1261 cacgatttcc gccacgaccg gggtaatggg cgccaggcgt ccccgtttgg cgtattcgat
     1321 ggacttcatc gtcaattcct ggctcgtgta ttgctccgtt tccgggttga tcatcgcctg
     1381 atacttcaac tgccagtcct tcttcatgaa cggcgccagg gtcgtccact gttcggcgcc
     1441 ccattcgtcg tacttgccga taatatccac gaaggccttc cacatctcca gccgcagccc
     1501 cccgagaaat acgccgccgt ccttggtggg atagaagccg tagagccaga ggcaggtgtc
     1561 gaggttgccg aagcgttcgt tgattacccc ctggtcggcg taccagtgga gcatatagtc
     1621 gttgagtcgc tcataggcct cgggagtgct gatgtcgatg gcctgccctt cgccggtcat
     1681 ttcccggtga tataccgccg ccagtatccc cagggcgccg aaggtcccgg aaaccgccca
     1741 ggcgatccac ggaccggcct tggtggaggt ggcatagggg acctccgcga tggtcttgcc
     1801 ctcgggaagg acctcgccgg ttgcgtactg gacgcccgag cgggcctggg ccacattgtc
     1861 gtaatcatac tggttccgac cgctttccgg accgaactgt ccgtagccgg ttatggaggc
     1921 atagatcagg cggggattca gtttcgccag ttcctcgtaa ccgatacccc agttatccat
     1981 cttccccgcc ggaaaggtct cgatgaggat atccgcctgg ccgaccaggg agcggagcat
     2041 atcccgcccg gcctccgttt ccagattcaa ggtgacgtgg tatttgttgc gtccttcctg
     2101 aagatatgcc attcccgtcc cgcggtgcat gacaccgttg ggggaatagc tccggaccag
     2161 atctcctccc ggcggttcga tgcggatcgt ctccgcccca agctccgcca gcaaagaagt
     2221 gcagtagggc ccggccatgc tccgggaact caggtcaatc acggtcaaat cgtccagcgc
     2281 ttcgggtttt tcaaagtttt ttgccgggtc gtcaagagcg cggatatgat cggcccagtc
     2341 ggcccatttc ggtcttttat gttcggtcat gtccctcacc tcctgatgat ggcgccagcc
     2401 tgtccgtccc aatcggacgg gggacggcgt ccctgcatgt ctttccattt ccccagaacg
     2461 ccatgcagat aaagttcatc gatctgcgcc tgattcctac ccaggatctt cgccatgatg
     2521 aattcattgt cgaagcctac cggtcgtacc gtccatttga tcttgggagg agtcttggac
     2581 atcatgacgg gaaattcgtg ctctacataa tacccgtaga gaggatcgtc gatctccttt
     2641 acgaatcccc gctggcggcg gtggggatcc tcgttcatgt ccttggcgtt atggaggcgg
     2701 gtggcggaaa aaccgaactc tttgcccagg gcctcgattt cgtcggcggt cttggtgcgg
     2761 gcccaggcgg cgatgatctt cagaatctcc agggtgttct catcctggag ccggacgctg
     2821 tgctcctgaa aacggggatc ggaggccaac tcctccctgc ccatggcccg gcacagacca
     2881 aggaattcct ccggcgccgg ggcggccagg gcggccagac ggtcgtcggc acagaggaag
     2941 gtgtcggcgg gacagatgca ttgatcgcgg ttgcccgccg cctccacgcc cttgccggtc
     3001 aactgctggt aaggccaaac ccagttcatg acccgcatga tgttctccgt ctgggacatc
     3061 tcgatgaact gtccccgacc ggtcttccgt cggtgatgaa gggcggccag gatggctacc
     3121 tcgcccatga ggccgccgta ataatcacag atccagatgg cctgcttaag gggcgggcgc
     3181 ttggggaagc tggacatcca ggcgtatccg gacagggcct gcgaagcccc gtcattggag
     3241 ggacggtcct gctcggcgta gaggccccac tggccgaagc cgtttttctc gatataaatg
     3301 atgccgggat ttacctcctt gatctgctca taaccgatat tccagcgtct ggttaccccg
     3361 ggacggagat tgaattcaat gatgtctgcc ttggccgcca gttccttgaa gatctgctgt
     3421 cccaaatcga tatgaaggtc gagggccatg taatacttgt tggggttgat gtgcatgaac
     3481 atgggcgact gttctttgta gaaccagccg aagggattga gagatcgcgt tacgtctccg
     3541 acggggggta gttcgatctt gatgacctcg gctcccattt ccgccaggaa ggacggaccg
     3601 gaaggaccga agaggagcgt acagacctcg aggacccgga cgcccttgag aggtccgggc
     3661 cgagagaatt ttttcttcac ataatccttg aaaacatcag gcataaggtt ccctccttta
     3721 accgtgaagc aaaaaaataa ttatgatatt tgttcacccg atttctccga taactggaaa
     3781 tcaccccctt tacatgtgga ttgattgtac atagccagga aatagcggcg ccaaatctat
     3841 ctgagtttaa gtaacacggc agcaacgggg aagcaagaaa tatttaccct tttccagcgt
     3901 ttttattccc tccttcctca agcgcaatcc aggcgccggg gaagaagacg gtagttttcc
     3961 tccttgacaa ggtgcgcgcc atggcctata attttacgtg tgaatgtata attaaattgt
     4021 tcaggtgaag tcatgaccgc ctatctcgac aaaaatcgca tcctcgatgc cgcccgggtc
     4081 gtttttgccc ggcacggcta ccgccgggcc accctggccg atatcgtcca ccccctgggg
     4141 gtcactaaaa cggccatcta tcatcatttt tccggcggaa aagaagagat tttccatgcc
     4201 ttggtcctcc gggaagagaa ttccgtcctg accgacatgg aaagcgccgt cgccgccgaa
     4261 aaggaaccgc cggcaaaatt gcgggcgttg attctcgcta agctcaccca cttccagcaa
     4321 cttagggagt tgctcatggt cccccgtgat gtaggagagg aggtggcaca gctctatgcc
     4381 ggtcatgaga cctcttttcg ggcctcggag cgggcgatga tcgccgccat cctcaaggaa
     4441 ggccagcgca ccggcatctt ccgtcccata gatacggggc atttggcgaa aaatgtccag
     4501 acgatcctca atcgtctcga attgcccctg atctatgaag atacatccga ccatatggag
     4561 catgaggttg atgacctcct cgacatcctg ttccacggga tcatgacggc ctggggagaa
     4621 aaccatattc ggtaaaattt aaccggagag aaaacccatg actgaaatag aaaaaccgag
     4681 tgatgccgcg gccaagatca gtaaatggcg tcgggccggc cggtgggtga tgatcctgat
     4741 cctggccgcc gccatgcttt acgggggacg ttggctctac tggcggatcg gccatgtcac
     4801 caccaacgcc gcctatatca aggccgacat ggccaacgtc gtcccggaga tccccggcaa
     4861 gatcctgtcc attgcggtga aggaggggca gaccgtccgg gctggacagg tcctgctcca
     4921 tatcgatccg gaacagatgg atcgcca
//
LOCUS       DAOSMF010000138         4941 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_18598_length_4941_cov_28.328285, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000138 DAOSMF010000000
VERSION     DAOSMF010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4941
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18598_length_4941_cov_28.328285"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /gene="ruvX"
                     /locus_tag="PLT63_09840"
     CDS             <1..240
                     /gene="ruvX"
                     /locus_tag="PLT63_09840"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOD99063.1"
                     /translation="EKVNRFASLLENTLHLPVIRWDETLSTKEAEQILIETGIKKEKR
                     KAIVDRIAAGIILQNYLNSVQLNAVQNSPSPGRNL"
     gene            294..1268
                     /gene="mltG"
                     /locus_tag="PLT63_09845"
     CDS             294..1268
                     /gene="mltG"
                     /locus_tag="PLT63_09845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOD99064.1"
                     /translation="MLAFIIVLSGAVIAVNFVYRSVDDSSRTVLIDIPRGDGFLQIID
                     KLDQAGLVTNRPLFTTLAILKGAARQIRAGEYELSSSMTPVEIIAKLVKGEIKFYMVT
                     IPEDLTVREIAALLAVNKLVKESNFLDLARDRRFVASLGIAGDSLEGYLYPETYKLDR
                     SMGSREIIQIMNRQFWKRFTPEMRKRAAELGMTINEVVTMASLIGKETGFKDEKPLIS
                     AVFHNRLGMGMKLQSDPTAVYDLEEFDGKIRRSHLLRDAPHNTYRIKGLPPGPIANPD
                     IDSLKAALYPVKTDYLYFVSNNNGSHDFSSNLTAHCQAVSRYQILRKK"
     gene            1351..1806
                     /gene="mraZ"
                     /locus_tag="PLT63_09850"
     CDS             1351..1806
                     /gene="mraZ"
                     /locus_tag="PLT63_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOD99065.1"
                     /translation="MSVFRGQYYHTIDGKGRIIFPARLREVFAEKSNGQLVITKLNEY
                     LLVFPYNEWEIIEQKIWHQSLLRRQVRDFQRFFMSSAVDSTLDSQGRLLIPPHLREYA
                     HLEKDIVLVGMLRNVEIWDKESFEVEMKRMGEQNADSQGFLDYMADLGI"
     gene            1822..>2213
                     /gene="mraW"
                     /locus_tag="PLT63_09855"
     CDS             1822..>2213
                     /gene="mraW"
                     /locus_tag="PLT63_09855"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HOD99066.1"
                     /translation="MDFFHQPVLLNEVIASLGCRAGGIYIDGTVGGAGHAIEILKQSS
                     PDGLLIGIDADAEAIHRAAKRLYPFRDRVNLVRGNYAAMGDILLALNIDRVDGILLDL
                     GVSSHQLDSAARGFSFAKEALLDMRMDTR"
     assembly_gap    2214..2313
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2369..2716
                     /gene="ftsL"
                     /locus_tag="PLT63_09860"
     CDS             2369..2716
                     /gene="ftsL"
                     /locus_tag="PLT63_09860"
                     /inference="COORDINATES: protein motif:HMM:NF016860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HOD99067.1"
                     /translation="MAVSEALKGTYPQERKKAPEAKSAKLIHVHKYSIYIVAALLLMS
                     VLLCYVASHLQMTRLEYRIAEEISRKERLLEEQKKLKLEIATLKSPQRIAAIARERLQ
                     MSHPDKDQVIILK"
     gene            2735..4741
                     /locus_tag="PLT63_09865"
     CDS             2735..4741
                     /locus_tag="PLT63_09865"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HOD99068.1"
                     /translation="MREEKKWLKFRIVTLLVFFLVLFVALITRAFQLQILSGQALKTM
                     AAKQHLKTLEVPPERGIIFDRNGEKLAATVLVDSVCADPSKLRNPQEVAEKIGPLLGI
                     DRTTLAKKFSSAKNFCWLARRVSPEQVKKVEALNLEGVFLIKEPKRFYPNGELAGHLL
                     GFVGLDSTGLEGLERKYNHYLQGAPERLVLIRDAKGKKLHPQIEKPAAEEKTSSHLIL
                     TIDSRIQHLVEGQLKAAVQDKGAKGGYAIVMDPRTGEILALANQPSFDPNYFTGAGAS
                     PGKNCAITDVYDPGSTFKPFLVASALEEKVVRENDRIFCENGHYAIAGRVIHEAQRKR
                     HGVLTVREIIKYSSNIGSAKIAERMGSERFYRYIAKFGFGSKTGIDLNGESAGLLRPH
                     PNWTRVDLTRIAFGQGISITPIQLITALSALANNGVLMKPYVIKGVVDRKGQIIKEFH
                     PTMVRTVISPATAKRITSMMTDVVGAEDGTGKRARIENVNVAGKTGTSQKFDFARHVY
                     SSERVRTSFMGFFPTEAPQVAILVVLDEPQRDRWGGVASAPVFKNIGEQILNSYKTHI
                     REQHPLDERDAIPPRKEAVRLVAASPAPGMALTGAAVGSEEGDESVMPDFRGLTIRDA
                     LRKAKGKNIEVKVVGNGWAVVQHPLPGTPLAGVSSCAITFSTGQ"
     gene            4750..>4941
                     /locus_tag="PLT63_09870"
     CDS             4750..>4941
                     /locus_tag="PLT63_09870"
                     /inference="COORDINATES: protein motif:HMM:NF013396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HOD99069.1"
                     /translation="MQLAAVLQNIDVIMIQGSTWGQVNSICYDSRRCERGSLFVAIPG
                     LKTDGHRFIPEALSRGASFI"
BASE COUNT         1165 a         1386 c         1319 g          971 t
ORIGIN      
        1 gaaaaggtaa accgtttcgc ctcgctactg gaaaacaccc tgcacctgcc cgtcatccgt
       61 tgggatgaaa ccctttcgac caaggaagcg gaacaaatcc ttatcgaaac cggaatcaag
      121 aaggaaaaga gaaaggccat tgtggataga atcgccgccg gcatcatcct gcagaattac
      181 ctcaattcag ttcaactgaa tgctgttcag aattcccctt cccccggacg caatctatag
      241 atccccacac cttcatgaag tcgagaaaag acagaaaatt taagacgctg ttattgttag
      301 ctttcattat tgttctttcc ggagcggtca tcgcggtcaa cttcgtctat cgatccgtag
      361 acgattcctc ccgtacggtc ctcatcgata tcccccgcgg cgacggtttt ttgcagatca
      421 tcgacaagct cgatcaggcc ggcctggtaa ccaacaggcc gctcttcacc acactggcga
      481 tcctcaaagg ggcggcgcga cagatccgtg ccggagaata tgaactctcc tcatcgatga
      541 cacccgtcga aatcatcgcc aaactggtca agggagagat caagttttac atggtcacca
      601 tcccggaaga cttaaccgtc agagagattg ccgccctgct ggcggtaaac aagctggtca
      661 aggaaagcaa cttcctcgac ctggcccgtg acagaagatt cgtcgcttcc cttggcatcg
      721 ccggggacag cctggagggc tacctctatc cagaaacata caaactggac cggtccatgg
      781 ggtcaagaga gatcatccaa atcatgaacc ggcaattctg gaagagattc accccggaga
      841 tgcggaagcg ggcggcggaa ctgggcatga cgatcaacga ggtcgtaacg atggcctccc
      901 tcattgggaa ggaaacgggg ttcaaggacg agaaaccttt gatttcggct gtgtttcata
      961 atcgcctcgg gatggggatg aagctgcaaa gcgacccgac ggccgtctat gacttggaag
     1021 agttcgacgg taaaatcagg aggagccatc tcctcagaga cgccccccac aatacctacc
     1081 gcatcaaggg gttgcctccg ggacccatag ccaatcctga tattgattca ctaaaagcgg
     1141 cgctttatcc ggtcaagacg gattatcttt attttgtatc aaacaacaat ggttctcacg
     1201 atttttcttc caacctgacg gcccactgcc aagctgtttc aagataccag attctcagga
     1261 aaaaataaca attttccctt gacaaaccag atggcgccct ttatagtgcc cacagtggag
     1321 tgaagtggag cattgtggag gaaacattcg gtgtctgtat ttcggggaca atattaccac
     1381 accatagacg gcaagggaag gataattttc cccgcccggc tgcgggaggt ctttgcggaa
     1441 aagagtaacg gccagctcgt catcacgaag ttgaacgaat atctcctggt cttcccgtat
     1501 aatgagtggg agatcatcga gcagaagatc tggcatcaat cccttctccg caggcaggtt
     1561 cgcgatttcc agcgtttttt catgtccagc gccgtggatt ccaccctcga cagtcaggga
     1621 cgcctgctca tcccccccca tctgcgggaa tatgcccatc tggagaaaga catcgtcctg
     1681 gtggggatgc tccgcaatgt ggaaatctgg gacaaggaga gtttcgaggt ggaaatgaaa
     1741 agaatgggag aacaaaacgc cgactcccag ggctttcttg attatatggc cgatctggga
     1801 atctgacaag gaccccatgg aatggatttt tttcaccagc cggtgctcct caacgaagtc
     1861 atcgcctccc tcggttgccg cgccggtgga atatatatcg acggcacggt gggtggcgct
     1921 ggacatgcga ttgaaattct taagcaaagc tctcccgatg gactgttgat cggcattgat
     1981 gccgacgccg aagcgatcca cagagccgca aagcggctgt atccctttcg tgatcgggtc
     2041 aacctggtac ggggtaatta tgccgccatg ggcgatatct tattagcatt gaacatcgac
     2101 cgcgttgacg gcatcctcct ggatctggga gtctcatccc accagttgga tagcgccgca
     2161 cggggattca gtttcgccaa ggaggccctt ttggacatgc ggatggacac ccgnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnggccaat ccccgctccc ggagcgccaa
     2341 gctccgcacc gtggaaagga tatgaaccat ggccgtcagt gaagccctga aggggacgta
     2401 tccccaggaa cggaagaaag ccccagaggc caaatcagca aagctgattc atgttcataa
     2461 atactccatt tatattgtcg cggccctgct cctcatgtcg gtgctcctgt gctatgtcgc
     2521 ctcccatctc cagatgacca ggctggaata ccggatcgct gaagagatca gccgcaagga
     2581 acgactgcta gaggagcaga agaagctcaa actggaaatc gccaccctga agtcgccgca
     2641 acggatcgcc gccatcgcca gagaaaggct gcagatgagc catccggaca aggatcaggt
     2701 cattatactc aaataattat ttttggtaag ggctatgcgg gaagaaaaga agtggctaaa
     2761 gtttcgtatc gtgaccttgc tggttttctt cctggtcctg tttgtcgccc tcatcacccg
     2821 ggcctttcaa ctgcagatcc tctccggtca ggcgctgaag accatggccg cgaagcagca
     2881 tctcaaaacc ctggaggtcc cgccggagcg cggcatcatc ttcgaccgca acggcgagaa
     2941 gcttgccgcc accgtcctgg tggattccgt atgcgccgat ccctccaagc tccggaatcc
     3001 tcaggaggtt gcggaaaaga tcggaccgct cctcggcatc gaccggacga ccttggcgaa
     3061 aaaattctcc agtgccaaaa atttctgctg gctggccagg cgggtctccc ccgaacaggt
     3121 taaaaaggtg gaggccctca atctggaggg ggtctttctt atcaaggaac cgaaacgctt
     3181 ctatcccaac ggcgagctgg ccggacatct cctcgggttt gtgggtctgg actccacggg
     3241 actggagggt ctggagcgga aatacaacca ttacctccag ggcgccccgg aaaggctggt
     3301 tctgatccgc gacgccaagg gcaaaaagct gcatccccaa attgagaagc cggcggcgga
     3361 agaaaagaca agcagtcatc tcattctgac catcgacagc cggattcagc acctggtgga
     3421 agggcagctc aaggcggccg ttcaggacaa aggcgccaaa ggcggttatg ccatcgttat
     3481 ggacccccgc accggcgaga tcctcgccct ggccaatcaa ccaagcttcg atcctaacta
     3541 cttcaccggc gccggcgcct ctcctggtaa aaactgcgcg attaccgatg tctatgatcc
     3601 cggctccact ttcaagccct ttcttgtcgc atccgctctg gaggaaaagg tggtcaggga
     3661 aaacgatcga atcttttgcg aaaacggcca ctatgccatc gctggacggg tcatccacga
     3721 agcccagcgg aagcgccacg gcgtcctcac tgtaagagaa atcatcaaat attccagcaa
     3781 tatcggctcg gcaaaaatcg ccgaacggat gggaagcgaa agattttacc gctacattgc
     3841 caaattcggc ttcggcagca aaacgggtat cgatctgaac ggagaatccg ccgggctcct
     3901 ccgcccgcat ccaaactgga cccgcgtcga tctgacccgg atcgccttcg gccagggcat
     3961 ttccatcacc cccatccagc tcatcaccgc cctgtccgcc ctagccaaca atggcgtgtt
     4021 gatgaagccg tacgtgatca agggtgtggt ggaccgcaaa gggcaaatca tcaaagaatt
     4081 ccatcccaca atggtgagaa cggtcatctc cccggccacc gccaagcgta tcacctccat
     4141 gatgacggac gtcgtcggag cggaggacgg cacgggcaaa agggcccgga tcgaaaacgt
     4201 caacgtcgcc ggcaaaaccg ggacctccca gaaattcgac tttgcccggc acgtctattc
     4261 ctcggaacgg gtccggacct cctttatggg atttttccct accgaggcgc cgcaggtggc
     4321 catcctggtg gtcctggacg agccccagcg ggaccgctgg ggaggcgtgg cttcggcgcc
     4381 ggtattcaag aatatcggcg agcagatcct caacagctac aaaacccata tccgggagca
     4441 acatcccctg gacgaaaggg atgccatccc tccccgcaag gaggccgtgc gactggttgc
     4501 cgcctcgcct gcgcccggca tggccctgac gggcgcggca gtgggcagtg aagaaggaga
     4561 tgaatccgta atgccggatt tccgggggct gacgatccgg gacgccctcc ggaaggccaa
     4621 gggcaagaac atcgaggtca aggtagtggg gaacggctgg gcggtggtcc agcaccccct
     4681 gcccgggacg ccgctggccg gcgtcagttc ctgcgccata actttcagta ccggacagtg
     4741 aggcatagcg tgcaattggc agccgttctc cagaacatag atgtgatcat gatccagggc
     4801 agcacctggg ggcaggtgaa ctccatttgc tacgattcgc ggcggtgtga gcggggcagc
     4861 ctgttcgtgg ccattcccgg gttgaagacc gacggccacc gcttcatccc cgaggcgctg
     4921 tcccgggggg caagcttcat t
//
LOCUS       DAOSMF010000139         4864 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_18976_length_4864_cov_20.065502, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000139 DAOSMF010000000
VERSION     DAOSMF010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4864
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18976_length_4864_cov_20.065502"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="PLT63_09875"
     CDS             <1..694
                     /locus_tag="PLT63_09875"
                     /inference="COORDINATES: protein motif:HMM:TIGR04085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HOD99070.1"
                     /translation="EGVEFIQFFPIVERIPAGSSGRHGLRLGAPPALDRPEANTAVTP
                     WSVEPEAYGDFLNMVFAAWVRADVGKIFVMNFEWMLNAWMGYASPVCLFARECGRSVV
                     LEHNGDVYACDHHVYPEYRLGNILTDNPLQMVSRSLAAGFGRAKETTLPHRCQECDVL
                     QLCRGGCPKHRFLTTYDGEPGLNYLCPGYGKFFRDSKKYLQVFRRLLENNLPPSLVMD
                     AVKGPLVIKRNP"
     gene            complement(816..2000)
                     /locus_tag="PLT63_09880"
     CDS             complement(816..2000)
                     /locus_tag="PLT63_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99071.1"
                     /translation="MKIRWKPINRAGELNRSTGDNLLANGLILVLLLAVLLLIPGGAA
                     AADAVTGGADKTTAATTYASASEANRTTPAAGAGKGDGQAREAVSDKCRELTSRIDID
                     LKEVVKAGCQPSQAQIAKMMDNPVGSLVFVANQFYWTQLKGPHIDGSLDAYAYKLIPT
                     FPIPIGESWNLINRPVFSVMSVPFKKEVGNLIGMGPSEIRQQPGLFSTIRDPFGRTTG
                     IGDFSYVGLFSPKKPDKVGTGIFVWGLGPTFIFPTASEDLLGQGKYQAGPAGVAAYIG
                     KKWALGLFPQHWWSYAGDSAREHTNLTNVQYFIYYNVTDTVKVGMGPNVTIDWTASGG
                     NNAVTLPVGLGVNWMTKVGKLPVRFGVEGYYSVIHPVDSMGSRFSLKFTITPVIPTFM
                     LF"
     gene            complement(2246..4684)
                     /locus_tag="PLT63_09885"
     CDS             complement(2246..4684)
                     /locus_tag="PLT63_09885"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HOD99072.1"
                     /translation="MMQKPGVSSRNVLFFCSQLMLSQLLCLPPAGASDILPRPEQPFK
                     GHIGRTVKDSVPDFPKPVTAPKGAPNVLIIMTDDVGFGASSTFGGPVPTPTFDRLADN
                     GLRYTAFHTTALSSPTRAALLTGRNHHTAATGVIMEMGTGYPGYNTMMPKSVGTFAEV
                     LKQNGYGTSWYGKNHNVPDWQTSQAGPFDLWPTNLGFEYFYGFLCAETNQWRPALFEG
                     TKPVEPPYHDKNYILDRDLADHAIAWIKSQHAMAPDKPFLAYYMTGTSHAPHHAPPEW
                     IAKFKGRFDQGWDKVREETFRRQQAMGIIPKNAKLTPRPAEIPAWDTLTADQKRLYAR
                     MMEVYAAALSHADHQIGRVVQAVADLGKLDNTLVIYIQGDNGASAEGRLNGMFNENTI
                     FNGVKEEQFVDIVKYMDKLGSLETYNHYPAGWAHAMDTPFQWTKQIASHFGGTRNGLV
                     ISWPRQIKDKGGIRSQFHHVIDIAPTVFAAAGVEMPAILNGVAQKPIEGVSMMYSFDN
                     PKAPSTHRTQYFEMFGNRGIYSDGWIASTTPQVLPWANTPRDPKRVTLDDANWELYNL
                     NEDFTQSVDLAQKEPKKLREMQDLFWAEAARYNVLPLDNDRISRFDVRIRPSVTAGRN
                     SFTYYPGMIRIPEGAAPDLKNKSFRITADVDIPAKGADGVLLTQGGQFGGYGLYVFKN
                     RPVFVYNFVGLATYRVAANETLQPGKHRIAFEFTYDGGGIGKGGAGVLSVDGRKVGEG
                     RIAHTNAVRISLEECFDIGQDTGTAVSKDYAERMPFKFTGGLEKVQVDLKPVPLSSAN
                     QKKIRKAQVDVTAR"
BASE COUNT          884 a         1438 c         1494 g         1048 t
ORIGIN      
        1 agagggggtc gagttcatcc agttctttcc catcgtcgaa cggatccccg ccgggtcctc
       61 cgggcggcac ggtctgcgcc tgggggcacc cccggctctg gaccgcccgg aggcgaacac
      121 cgccgtgaca ccctggtccg tggagccgga ggcctacggc gacttcctga acatggtctt
      181 cgctgcctgg gtaagggccg acgtcgggaa gatattcgtc atgaatttcg aatggatgct
      241 caacgcctgg atgggatacg cctcccccgt atgcctcttt gcccgggagt gcggccggtc
      301 cgtggtcctg gagcacaacg gcgacgtcta cgcctgcgat caccatgtct atcccgaata
      361 tcgcctgggg aacatcctga cggacaatcc tctgcagatg gtctccagat ccctcgccgc
      421 cggcttcggc cgggcgaagg agacaactct gccccaccgt tgccaggagt gcgatgtcct
      481 gcaactatgc cgggggggat gcccaaagca ccgtttcctc acgacctacg acggcgaacc
      541 ggggttgaac tatctctgcc cgggttacgg gaaattcttc cgggacagta aaaagtacct
      601 ccaggtcttc cgccggctct tggaaaacaa cctccccccc tccctggtca tggacgccgt
      661 caaggggccg ttggtgatta aacggaaccc ctgacggacg gttgacgtaa acaccccccg
      721 gaaccctgat cataaaagtg atcccgtggg gaggggtgcc ttggcgtgcc cgatgtgttc
      781 ggtccgctaa tggcggaacc gcctccggcc gccgtctaaa aaagcatgaa ggtcgggatc
      841 accggggtga tcgtaaactt gaggctaaag cgggaaccca tggagtccac cgggtggatg
      901 accgaatagt acccctcgac gccgaagcgg acggggagtt tgcccacctt ggtcatccag
      961 ttgaccccca ggcctacggg taaggtcacc gcattgttgc cgccgctggc cgtccagtcg
     1021 atggtgacat tgggccccat cccgaccttg acggtatcgg tgacgttgta gtagatgaag
     1081 tactggacgt tcgtcaggtt ggtgtgctcc cgggcactgt cgcccgcata ggaccaccaa
     1141 tgctggggaa acaggcccaa cgcccatttc ttgccgatat aggccgccac ccccgccggt
     1201 cccgcctggt acttgccctg tcccagaagg tcttcgctgg ccgtgggaaa gatgaaggtc
     1261 ggccccaacc cccagacgaa gatccccgtg cctaccttgt cgggtttttt cggtgaaaac
     1321 aggccgacat aggagaaatc cccgattccc gtcgtccgcc cgaaggggtc gcgaatcgtc
     1381 gagaaaaggc ccggctgctg gcggatctcc gacggtccca tcccgatcag attgccgacc
     1441 tccttcttga aggggacact cattacggaa aaaaccggcc ggttgatcag attccacgat
     1501 tcgccgatgg gaattgggaa ggtgggaatc agtttgtagg cgtatgcgtc gagactcccg
     1561 tcgatgtggg gacccttgag ctgggtccag tagaactgat tggcgacgaa gacgagactt
     1621 ccgacgggat tgtccatcat cttggcaatc tgggcctggg atggctggca tccggccttg
     1681 acgacctcct tgagatcgat atcgatgcgg ctcgtcagct cccggcactt gtcggagacc
     1741 gcctcccggg cctggccgtc gccttttccc gctcccgcgg cgggggtggt cctgttcgcc
     1801 tcgctggcag aggcgtaggt tgttgccgca gtcgtcttgt ctgccccgcc ggtcacggcg
     1861 tcggcggcgg cggccccgcc ggggatcagc agcaataccg ccaacagcaa cacgaggatc
     1921 aatccgtttg ccaggagatt atcgcccgtg gaccggttga gctccccggc acggtttata
     1981 ggtttccaac gaatcttcat agagatctcc atttattgag ggtgtgagag gcggctccag
     2041 gccgtctccc ataccggtta aaaacatcaa ggtttgacga catcgtaaaa agtccatatg
     2101 cggcgaaagc gcttcatcct tcgtcactgc gacgtacgca caagtacgcc tcattcctca
     2161 ggattcgcgc gccttgcctc tggagctttt tacttcgtcg tctccatcat gactttttac
     2221 taattcatca aggtttgcct tgatactatc gtgccgtaac gtccacctgg gccttacgga
     2281 tcttcttttg gttggcactg ctcaggggaa cgggcttcag gtccacctgt accttttcca
     2341 ggccgccggt gaacttgaaa ggcatccgct cggcgtagtc tttgctgacc gccgttcccg
     2401 tatcctggcc gatgtcgaaa cactcttcca gggagatgcg cacggcgttc gtgtgggcga
     2461 tgcgcccctc cccgaccttc cggccgtcca cggagaggac cccggcgccg cccttgccga
     2521 tgccgccgcc gtcataggtg aactcgaagg cgatcctgtg cttgcccggt tgcagggtct
     2581 cattcgcggc gacacggtac gtggccaggc cgacgaaatt gtagacgaag acgggccgat
     2641 tcttgaatac gtagaggccg tatccgccga actggcctcc ctgggtgagg aggacgccgt
     2701 cggcgccctt ggcggggata tctacatcgg cggtgatgcg gaatgatttg ttctttaagt
     2761 ccggggccgc cccttcgggg atacggatca tgcccggata gtaggtgaag ctattgcgtc
     2821 ccgccgtcac gctgggacgg atgcggacgt cgaagcggga tatccggtcg ttgtcgagag
     2881 gcaggacatt gtaccgggcc gcctccgccc agaagaggtc ctgcatctcc cgcagtttct
     2941 tcggttcttt ttgggccaga tcgacggact gggtgaagtc ctcgttaaga ttgtagagtt
     3001 cccagttggc atcatcgagg gtcacccgct tcgggtcccg gggggtgttg gcccagggca
     3061 ggacctgagg cgtcgtcgaa gcgatccaac cgtcgctgta aatgccgcgg ttgccgaaca
     3121 tttcaaaata ctgggtccgg tgcgtcgagg gggccttggg gttgtcgaag ctgtacatca
     3181 tgcttacgcc ctcgatcggt ttctgggcga cgccgttgag gatcgccggc atctcgaccc
     3241 cggcggccgc aaagacggtc ggggcgatgt cgatgacatg gtgaaactgg ctgcggatac
     3301 cgcccttgtc ctttatctgc cggggccagg agatgacgag gccgtttctc gtcccgccga
     3361 aatgggaggc aatctgtttg gtccactgga acggcgtatc catggcatgg gcccatcccg
     3421 ccggatagtg gttgtaagtc tccagagagc ccagtttgtc catgtacttc acgatatcca
     3481 cgaattgctc ctccttgacg ccgttgaaga tggtgttctc gttgaacatc ccgttcagcc
     3541 ggccttcggc gctggccccg ttgtcgccct ggatgtagat gaccagggtg ttgtccagct
     3601 tgccgaggtc ggcgacggcc tgtacgaccc gcccgatctg gtggtccgca tgggaaaggg
     3661 ccgcggcgta gacctccatc atccgggcgt agagccgctt ctgatccgcc gtgagggtat
     3721 cccaggcggg gatttcagcc gggcggggcg tcaacttggc gttcttggga ataatgccca
     3781 tggcctgctg gcggcggaat gtctcctccc ggaccttgtc ccagccttga tcgaaccgcc
     3841 ccttgaactt ggcgatccac tccggcggcg catggtgggg cgcatgggag gtccccgtca
     3901 tgtagtaggc cagaaagggc ttgtccggcg ccatggcgtg ctgcgacttg atccaggcga
     3961 tggcatgatc cgccaggtcc cggtcgagga tgtagttctt gtcgtggtaa ggaggttcta
     4021 ctggtttcgt cccttcgaag agggccggcc gccactggtt ggtctcggcg cagaggaagc
     4081 cgtagaaata ctcaaacccg aggttcgtcg gccagagatc gaacgggccg gcctggctgg
     4141 tctgccagtc ggggacgttg tgattctttc cataccagga ggtgccgtag ccgttctgct
     4201 tcaggacctc ggcaaaggtg ccgacgctct tcggcatcat cgtattgtag ccggggtagc
     4261 cggtccccat ctccatgatg acgcccgtgg ccgccgtatg gtggttccgt cccgtcagga
     4321 gggccgcccg cgtcggcgaa ctgagggccg tcgtatgaaa tgccgtatag cgcagtccgt
     4381 tgtccgcaag gcggtcgaag gtcggcgtcg gtaccggtcc gccgaaggtg gagctcgccc
     4441 cgaaacccac gtcatcggtc atgatgatca gaacgttcgg ggcccctttc ggggccgtta
     4501 ccggcttggg gaagtcggga accgagtcct tcaccgtcct gccgatatgg cctttgaaag
     4561 gctgctccgg ccggggcagg atgtcacttg cccccgccgg aggcaggcag agcaattgtg
     4621 acagcataag ctgggaacag aaaaagagaa catttcggga cgaaactcca ggtttctgca
     4681 tcatcgcata actcctttct atggtatatt gatgtgtggc aggatacttt gattgctggt
     4741 gcataaatta aggtggaccc atttgtcaag ttgctatatt ctgtcaaaca caaatttatc
     4801 aatatagtta agttacaccc tttgctcttg acaggcagtc gtaaattcct tgttaacggc
     4861 ccgc
//
LOCUS       DAOSMF010000140         4861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_18993_length_4861_cov_22.737620, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000140 DAOSMF010000000
VERSION     DAOSMF010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4861
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18993_length_4861_cov_22.737620"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="PLT63_09890"
     CDS             complement(<1..272)
                     /locus_tag="PLT63_09890"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOD99073.1"
                     /translation="MINLTINGVSVSVAKGTTVLEAARYLGFPIPTLCHKEGLTPYGA
                     CRLCVVEIGKAPQTRLVSSCTYPVEEGLIVRTASERVVKARRIIIEL"
     gene            complement(274..3516)
                     /locus_tag="PLT63_09895"
     CDS             complement(274..3516)
                     /locus_tag="PLT63_09895"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOD99074.1"
                     /translation="MYTFQTIAEFKAYRKSIMDAEDKRKPCLVICGGTGGQASGSNDL
                     IRIIKRQILEQNLHDQLSLRITGCLGFCEMDPFIIVEPGYNLYPKLKMEDVPRIVEAA
                     VKGEVIEELLYREPGSTVVRFSEQDIPFFQGQSRIVLGQNQHVDPIRINDYLKIGGYA
                     ALEKALAKPDRKWIVDEILKSKLRGRGGAGFMTGKKWEMARSQHRPFGRKYVICNADE
                     GDPGAYMDRSMLEGNPHAIIEGMIIAALAMKATHGIIYVRTEYPLAIKHATIAIREAG
                     RMGLLGKNILGQGFDFEIEIFHGAGAFVCGEETALIKSIEGKMGEPRQRPPYPIAKGL
                     FGCPTCINNVETLANVPYIINHGADAYLKIGTQNNAGTKIFSLVGKIKNTGLVEVPLG
                     TTISKVVYEIGGGASGTAKIKSIQTGGPSGGCIPASMFDLPIDYDSLAKVGSIMGSGG
                     MIVMDSNTCMVDVAKYFMNFLRDESCGKCFTCRKGTQRLYEILEDITLGKGTPEHLVL
                     LDELARTVKDTTMCGLGQTAANPVLSTLKYFREEYERHINDKRCDAFVCKQLTGAPCQ
                     AACPVDTEPWRYVAMIEKGDYEEAYRVIRSANPFPAVSARVCDRKCESRCSLGTSGGE
                     AVAIRALKRFVTDRVKPSVYKPVPAKKKRQKVAVIGAGPAGITAAHRLSLLGYGVTIF
                     EAEDRPGGMLTCSLPAYRLPQDIVDKEIKTLVNKNIKIEYGRVLGKDITIHDLFVDKY
                     DAVFLAFGAHESMRLDLKHEDVDGIYPSIQFLKAFNMRGEELARGHVGVIGGGNSAVD
                     AARVALRQKDVASVSLFYRRTREEMPAYEEEVEAALEEGVRIETLISPVKILVRQEEI
                     EAAVREGVELETLVSPIKIHSRKGHLVDIECIRNQLGDVDATGRHKPVPIPGSEFKVK
                     LDTLIVAIGEKPKSEFLAGLGLEIDKSGRIKVNPKTLATNLRGVFAGGDLVTGPNTVI
                     DAIAAGKKAAESIDLYLQKKPLIKPARPKLPDVYIAPAPGDESESITRVRAVNPALTP
                     AERVKNFREVEMSLPENSAREESRRCLRCDLAFTEEKSAAMPVQAAMEGA"
     gene            complement(3537..4388)
                     /locus_tag="PLT63_09900"
     CDS             complement(3537..4388)
                     /locus_tag="PLT63_09900"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOD99075.1"
                     /translation="MSSEEIEKIISSHADRQGSIIAILETIQNRYRYLPEEALRIVAR
                     RTGRSLVDVYGVATFYKAFSLKPRGKHLISACLGTACHVRGGQAIAEEFMQQLQITAG
                     ETTPDKEITFETVNCLGACALGPTVVVDGHYYPHVTKSQVREIIADTLEGMEKIAAAT
                     NRRIFPIHVACPTCKKSLMDYDQRLDGHPSIKCVVSFNGRKGRLGLSSLYGSRAIASE
                     LEIPMNELSRFYCPHCHEELPHYSNCAECGSPMAALYIRAGCAYEVCTSRGCHGHLLN
                     LDQTNMT"
BASE COUNT          939 a         1404 c         1368 g         1150 t
ORIGIN      
        1 agctcgatga tgattcgtcg cgccttcacc acgcgctccg aggcggtgcg cacgattagc
       61 ccctcttcga cgggataggt gcaggatgaa accaatctgg tttgcggcgc cttgccgatc
      121 tccaccacac agagtcggca ggccccgtag ggtgtaagtc cctccttgtg gcacagcgtg
      181 ggaatgggga aacctaaata ccgtgcggcc tcaaggacgg tggtgccctt tgcgacggat
      241 acagataccc cgttaatcgt gaggttgatc atgttacgct ccttccatcg cagcctgcac
      301 cggcatggcc gcggactttt cttcggtgaa cgccaggtcg cagcgcaggc agcgtctgct
      361 ctcctcccgg gccgagtttt ccggcagaga catttccacc tcccggaaat tctttacccg
      421 ctcggccggc gtcagggcgg gattcacagc ccggaccctc gttatcgatt ccgattcatc
      481 gccgggcgcc ggtgcgatgt aaacatcggg aagctttggt ctggccggct ttatgagtgg
      541 tttcttctgc aggtacaaat cgatgctttc cgccgccttt ttccccgccg caatggcgtc
      601 gataaccgtg ttcggaccgg tcaccagatc gccgcccgcg aatacccctc tcagattggt
      661 ggcgagggtt ttggggttca cttttatgcg gccgctcttg tcgatttcca gacccaggcc
      721 ggccagaaat tcgcttttcg gtttttcgcc tatggccacg atgagcgtat cgagtttgac
      781 cttgaactca cttccgggga tgggcaccgg cttgtgcctt cccgtggcgt ccacatcgcc
      841 cagttggttg cgaatgcact cgatgtccac cagatggcct tttctggaat ggatttttat
      901 gggtgaaaca agcgtttcca gttcgacgcc ttcccggacg gccgcctcga tttcctcctg
      961 gcggacgaga atcttcacgg gtgagatcag ggtttcaatc cgcacgcctt cctccagggc
     1021 cgcttcgacc tcctcttcat aggcgggcat ttcttcacga gtgcgccggt agaaaaggga
     1081 gacgctcgcg acatccttct gacggagggc gacacgcgcc gcatccaccg ccgaattgcc
     1141 tcccccgatg acgcccacat gtccccgcgc cagctcttcg cctctcatgt tgaaggcttt
     1201 caggaactga atggaaggat agatgccgtc aacatcttca tgtttgagat cgaggcgcat
     1261 gctttcgtgg gcgccgaagg ccaggaagac cgcgtcatat ttatcgacaa acaggtcatg
     1321 gatggtaatg tcttttccca gcacgcggcc gtattctatt ttgatgttct tgtttacgag
     1381 ggttttgatt tccttatcga caatatcctg aggcagtcga taggccggca gagagcaagt
     1441 cagcatgccg ccaggccggt cctccgcctc gaagatcgtc acgccgtatc ccagcaagga
     1501 aagacggtgg gcggcggtta tgcctgccgg accggcgccg atgacggcca ccttctgccg
     1561 cttcttcttt gcgggaacgg gtttatagac cgacggcttt acccggtccg tcacgaagcg
     1621 tttcaaggcg cggatggcta cggcctcccc tccgctcgtc cccaggctgc aacggctttc
     1681 gcatttgcgg tcacagaccc gggcgcttac ggccgggaac gggtttgcgg accggatcac
     1741 ccggtaggcc tcctcgtaat cgcctttttc gatcatggcc acataccgcc agggttcggt
     1801 gtccacggga caggcggcct ggcagggggc gcccgtcaac tgcttgcaga caaaggcgtc
     1861 gcaccgttta tcgttgatgt gccgctcata ttcctcccgg aaatacttga gcgtgctgag
     1921 gaccggattg gcggcggtct ggccgaggcc gcacatggtg gtatccttta cggtccttgc
     1981 caactcatcc agaagcacca gatgttccgg cgtacccttc ccgagggtga tgtcctcgag
     2041 tatttcataa agccgctggg tccccttgcg gcaggtgaag cacttgccgc aggactcgtc
     2101 ccgtaagaaa ttcatgaagt atttggccac atcgaccatg caggtgttcg agtccataac
     2161 gatcatgccg cccgatccca taatggaccc gactttcgcc aggctgtcat aatcgatggg
     2221 cagatcgaac atggaggcgg ggatgcagcc gccggaaggc cctcccgtct ggatggactt
     2281 gatcttcgcc gtgccggagg cgccgccgcc aatttcataa acgaccttgc tgatggtggt
     2341 ccccagggga acttcgacca gcccggtatt tttgattttc ccgaccaggg agaatatctt
     2401 cgtaccggcg ttgttctgcg tgccgatctt caaataggcg tccgcgccgt ggttgatgat
     2461 gtaggggaca ttcgccaggg tctccacatt gttgatgcag gtgggacagc cgaaaaggcc
     2521 tttggcgatg ggataggggg gacgctgtcg cggttcgccc atttttcctt cgatggattt
     2581 gatgagcgcc gtctcctcgc cgcagacaaa cgcgccggca ccgtgaaaaa tctcgatttc
     2641 gaaatcgaaa ccctggccca agatattctt ccccaggagc cccatccggc cggcttccct
     2701 gatggcgata gtcgcatgct tgatggccag gggatactcc gtgcgcacat agatgattcc
     2761 gtgggtggcc ttcatggcga gggcggcgat gatcatcccc tcgatgatgg cgtgggggtt
     2821 gccctccagc atgctccggt ccatataggc gccgggatct ccctcgtcgg cattgcagat
     2881 gacgtatttt ctgccgaaag gacggtgctg gctgcgcgcc atttcccatt ttttccccgt
     2941 catgaaaccg gccccgccgc gcccccgtaa ttttgatttc agaatttcgt cgacaatcca
     3001 tttccgatcc ggctttgcca atgccttttc cagggccgca tatcccccta tcttcaggta
     3061 atcattgatc cggatcggat cgacatgctg gttctgcccg agaacgatcc ggctttgccc
     3121 ttgaaaaaac gggatatcct gttcggaaaa gcggacgacc gtggagcccg gttcccggta
     3181 caggagttct tcgataacct cgcctttcac ggcggcttcg acaatccgcg gcacgtcttc
     3241 catcttgagt ttcggataca gattataccc cggttcgacg atgatgaagg ggtccatttc
     3301 acagaagcca agacatccgg tgatgcggag ggagagttgg tcatgcagat tctgctccaa
     3361 tatctgacgt ttgatgatcc ggatcagatc gttcgagccg ctggcctgac ccccggttcc
     3421 cccgcagatg acgaggcatg gcttccgctt gtcttcggcg tccataatgg atttacgata
     3481 cgccttgaat tcggcaatag tctgaaaagt atacatttta tttcccttta tcgatcttat
     3541 gtcatgttgg tttgatccag attcagcaga tggccgtgac agccccggct cgtacatact
     3601 tcataggcgc agccggcgcg gatgtacagg gcggccattg gtgagccgca ttccgcgcag
     3661 tttgaataat gcggcagctc ttcgtggcag tgggggcaat aaaaccggga caactcgttc
     3721 atggggattt caagttccga ggcaatggcg cggctgccat agagagaaga caggccgagc
     3781 cggcctttcc tgccgttgaa agaaaccacg cacttgatgg atggatggcc gtcgaggcgc
     3841 tggtcgtaat ccatgagact ttttttgcag gtcggacagg cgacatggat ggggaatatc
     3901 ctgcggttgg ttgccgcagc gatcttttcc atgccctcaa gggtatccgc gatgatttcc
     3961 ctgacctggc ttttggttac atgggggtaa taatgaccgt ccaccacgac ggtaggaccc
     4021 aaggcgcagg ccccaaggca gttgaccgtt tcaaaggtga tttctttatc gggggtggtt
     4081 tcaccggccg tgatctggag ttgctgcatg aattcctcgg caatggcctg gccgccacgg
     4141 acatggcagg ccgttcccag gcacgcggaa attaaatgtt tcccccgggg cttcaggctg
     4201 aaggccttat agaaggtcgc caccccgtaa acatcgacca ggctccttcc cgtccggcgg
     4261 gcgacgatcc tcaaggcctc ttccgggaga taccggtagc ggttctggat ggtctcaagg
     4321 atggcgatga tcgatccctg tctgtccgca tgacttgaaa taattttttc aatttcttcc
     4381 gatgacattt ctattccttt ccaggcaaag caatcccaac atcctcgccg tatttctcct
     4441 cgctgctacc gatagtcctc gcagtgccat acccccgctg ccggttttgg gtaggtgcaa
     4501 cccggataat gatcgcaatt gccgcaaagg ccaagcattc cggagtgggt gcgcgggatg
     4561 gatcctgggg atgttctctc cggcgacgag gcatcagccg caagttgaac cgtttcatat
     4621 tcttcgcaaa accggacacc gagcgcggca cttattatgg aaaccagcgc acaatcctgg
     4681 gaatttgcac aattcatgca caacccttgt ttctctgtga tcatggcatc ctcccgttgc
     4741 tgttatgtgc accaccacgg caaagatcat gccggcggga ccagggccag gacatcagaa
     4801 aagggaatcg gacggaacga tttgaattct gacggttaat gattacccgg gcggttgctt
     4861 a
//
LOCUS       DAOSMF010000141         4575 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_20532_length_4575_cov_24.310398, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000141 DAOSMF010000000
VERSION     DAOSMF010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4575
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20532_length_4575_cov_24.310398"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(258..>1061)
                     /locus_tag="PLT63_09905"
     CDS             complement(258..>1061)
                     /locus_tag="PLT63_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOD99076.1"
                     /translation="SIPVSGPEYLKYGGDTTCLEIRTKHDDIIIVDAGSGIRRLGNAL
                     MTEGRRDYNMFFTHAHWDHLLGFPFFKPIYYPGTEINMFGCPFAQISVQELISQVMAA
                     PHFPIEYQNIRCEISYHQTCGDRFSINEMTVTSFPLSHPNEGMGYRFEEDGKSFVFLT
                     DNELSYIHPGGRGFDDYVGFAAGADLLIHDAEFREDDYKQTWGHSTYNDAVRLALAAG
                     VKTLGLFHHNQERTDVGVDDILARSIAMVRECQGLLTCCAVYRDMQFIV"
     assembly_gap    1062..1161
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1162..3357
                     /locus_tag="PLT63_09910"
     CDS             <1162..3357
                     /locus_tag="PLT63_09910"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOD99077.1"
                     /translation="VRRVEKALQSLPGVEEAAVNLATGRATLRHRPDWAGLDEVGKTI
                     TATGYDYLGAPEEFRADPIAAAREEEIRDLRRRFAVGMTLSVVIFFGSMQHWFPFLAT
                     LPRTPLLIAMFILTTPVVFWVGSRFFTGAVKAARQGTTDMNTLVAVGAFSAYAYSALA
                     TFAPTLFTAAGVVPHVYYDGAAFIVTLIILGRLLEAGAKGKTSAAIQRLMELRPKTAR
                     VIRNGEERDIPIEGIIRGDLLLVRPGEKIPTDGVIVSGSSAVDEAMLTGESMPVPKEP
                     GHAVYAATINKSGAFTFTATKIGAETALARIIGLVEQAQGAKAPIQRLADRVAAVFVP
                     VVFAIGIAAFVVWYFFVPDGSFSRALLNFVSVLVIACPCALGLATPTAVMVGTGLGAE
                     RGILIKGGEALEKACRLTMVVFDKTGTLTRGEPQVTDILTGPGVSENELLRIALAIEA
                     ASEHPLGVAIVREGRLRGLSAEAAEAFEAAAGLGAQGRVAGQPCLLGNRRYLESFGFT
                     IRNWEKQEEHLAGAGKTGVYIARGKDIIGLIGLSDVPRDTAGEAVATLKDMGLKVAMI
                     TGDNVRTARAVGTDLRIDTVLAEVLPEDKAAEIYRLQKNGEVVAMVGDGINDAPALAA
                     ADIGIAIGAGADVAMEAGDMTLMREDLRTVPAAIRLSHQTMRVIRQNLFWAFIYNIIG
                     IPIAAGVLYPFGGILLNPEFAAAAMALSSVSVVSNSLRLRRVVRKGNGI"
     gene            complement(3286..3882)
                     /locus_tag="PLT63_09915"
     CDS             complement(3286..3882)
                     /locus_tag="PLT63_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99078.1"
                     /translation="MIPLLALLLTGGIFILWLVRKGATLTAREALVNYLDARRRGRTE
                     EAYCYLSAAARAVCSPAEYKEANSLGSGLIAELVGRHVSFAVERIEENPERAVAWVAV
                     TAPDFRLMLGEICRTLDPARLPEDSLAAQTFLCRRISGFLDTYQRDGLPLRTKVETYL
                     LHRETNGWKIAAAPLSDAIAFPYHPAEAQGVAHYGDGA"
     gene            complement(3904..4074)
                     /locus_tag="PLT63_09920"
     CDS             complement(3904..4074)
                     /locus_tag="PLT63_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOD99079.1"
                     /translation="MTTASRHICSICGYVYDPVLGDPEHDVPAGTAFEDLPADWTCPV
                     CGAGKDQFEPEG"
     gene            complement(4165..4560)
                     /locus_tag="PLT63_09925"
                     /pseudo
     CDS             complement(4165..4560)
                     /locus_tag="PLT63_09925"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
BASE COUNT          839 a         1402 c         1391 g          843 t
ORIGIN      
        1 ggcggccaaa tggctcacca tgcgccgatg gcttgaagcc aatcccggcg acgagctcgc
       61 gaaagatgtt cgaaccaaac tgacctcgga acccgagcgc tacgccgctt acacgcgcca
      121 atacctgggc tggggtgtgt tcgcgctgat gccgcggtga gggaaggggc ttaccctaag
      181 tcggcataag ttaccgggaa ggattttcat gctaccatgg cgaggctgtg gagataggaa
      241 ggtttttgca aagacttcta aacgatgaac tgcatgtccc gataaacggc gcagcaggtc
      301 aacaaaccct ggcactcccg aaccatggca atgctccgcg ctaaaatgtc gtccaccccg
      361 acgtcggtcc gctcctgatt gtggtggaag aggcccaggg tcttcactcc cgccgccagg
      421 gcgaggcgca cggcatcgtt ataggtggaa tgcccccagg tctgcttgta gtcgtcttcc
      481 cggaattcgg cgtcgtggat gaggaggtcg gcgccggcgg caaaaccgac gtaatcgtca
      541 aagccccggc cgccgggatg gatgtaggac aattcgttgt ccgtcagaaa gacgaaagat
      601 ttcccgtcct cctcgaagcg gtatcccatc ccctcgttgg gatggctcag ggggaaggac
      661 gtcacggtca tctcgttgat ggaaaagcgg tccccgcagg tctgatggta ggaaatttcg
      721 cagcggatat tctgatattc gatgggaaaa tggggcgccg ccatcacttg ggagatcagc
      781 tcctggacgg atatctgggc aaaggggcat ccaaacatgt tgatttcggt gcccgggtaa
      841 taaatgggct tgaagaaggg aaaacccagg aggtgatccc agtgggcgtg ggtgaagaac
      901 atgttgtagt cccgccgccc ttccgtcatc agggcgttac cgaggcgcct gatacccgac
      961 ccggcgtcca cgatgattat atcgtcatgt ttggtccgga tttcgaggca ggtggtgtcg
     1021 cccccgtatt tcaggtattc cggccccgac acgggaatgg annnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn ngtgcgccgc gtcgagaagg ccctccagtc cctgccgggg
     1201 gtggaagagg ccgccgtcaa tctcgccacg ggccgggcga ccctccgcca ccgccccgac
     1261 tgggcgggcc tggacgaagt gggaaagacc atcacagcaa cgggctacga ctatttaggc
     1321 gccccggagg aattccgggc cgatcccatc gccgcggccc gggaggagga gatccgggat
     1381 ctcaggcgcc ggtttgccgt gggcatgacc ctaagcgtcg tgatcttctt cggttccatg
     1441 cagcattggt ttcccttcct ggccaccctg ccccggacgc cgctgctcat cgccatgttc
     1501 atcctcacga cccccgtcgt cttctgggtg ggcagccgct tctttaccgg ggccgtcaag
     1561 gccgcccggc agggaaccac ggacatgaac accctggtcg ccgtcggggc cttcagcgct
     1621 tatgcctatt ccgccctggc caccttcgcc ccgacgctgt tcacggcggc aggcgtcgtg
     1681 ccccacgtct attacgacgg cgccgcattc atcgtcaccc tcatcatcct gggccgcctg
     1741 ctcgaggccg gggccaaggg gaagacctcg gcggccatcc agcggctcat ggaactccgg
     1801 cccaagaccg cccgggtcat ccgcaacggc gaggaaaggg atatacccat agaaggtatc
     1861 atccggggcg atctcctcct ggtccgtccc ggcgaaaaga tccccacgga cggcgtgatc
     1921 gtctccgggt cttccgccgt tgacgaagcc atgctcaccg gggaaagcat gcccgtcccc
     1981 aaagagccgg gccacgctgt ttacgccgcc acaatcaaca agagcggagc ctttaccttt
     2041 accgccacga agattggcgc ggaaacggcg ctggcgcgga tcattggtct cgttgaacag
     2101 gcccagggcg ccaaggcgcc catccagcgc cttgccgacc gggtggcggc ggtattcgtt
     2161 cccgtggttt tcgccatcgg tatcgcggcc tttgtcgtct ggtacttttt cgttcccgac
     2221 ggcagcttca gccgggccct cctgaacttc gtctccgtcc tggtcattgc ctgcccctgc
     2281 gccctcggtc tcgccacccc cacggccgtc atggtgggca cgggcctcgg ggcggagcgg
     2341 ggcattctca tcaagggagg cgaagccctg gaaaaggcct gccggctaac catggtcgtc
     2401 tttgacaaga cgggaaccct cacccgggga gaaccgcagg taacggatat cctgaccgga
     2461 ccaggggtgt ccgaaaacga gttgctccgg atcgccttgg ccattgaagc ggcctccgag
     2521 caccccctcg gcgtcgctat cgtccgggag ggacgcttgc ggggcctatc agccgaagca
     2581 gcggaggcat tcgaggctgc tgccggcctg ggggcccagg gacgcgtcgc cggtcaaccc
     2641 tgcttgctgg gcaaccgtcg ctatctggaa agtttcggtt ttaccatccg gaactgggaa
     2701 aagcaggagg agcatctggc cggcgcaggt aaaaccggcg tctatatcgc ccggggcaag
     2761 gacatcatcg gtctcatcgg cctgtcggat gtccccagag ataccgccgg agaggccgtg
     2821 gcgaccctta aagatatggg actgaaggtc gccatgatca ccggtgacaa cgtccggacg
     2881 gcccgggcgg tcggaacgga cctccggatc gacaccgtgc ttgcggaggt cctgccggaa
     2941 gacaaggccg cggaaattta caggttgcag aaaaacggcg aggtggtggc catggtgggg
     3001 gacggcatca acgacgcccc ggccctggcc gcggccgata tcggcatcgc catcggcgcc
     3061 ggtgccgacg tggccatgga ggccggcgac atgaccctca tgcgggagga tctccggacc
     3121 gtaccggcgg ccatccgcct gtcccaccag acaatgcggg tgatccgcca gaatctcttc
     3181 tgggccttca tctacaacat catcggcatc cctatcgccg ccggggtcct ttaccccttc
     3241 ggcggcatcc tcctcaatcc ggaattcgcc gccgccgcca tggccctaag ctccgtctcc
     3301 gtagtgagca actccctgcg cctccgccgg gtggtaagga aaggcaatgg catctgaaag
     3361 aggcgccgcc gcgatcttcc agccgtttgt ctcccggtgc aggagatagg tctccacctt
     3421 tgtccgcaac ggcaggccgt cccgctgata tgtgtcgagg aagccgctta tccggcggca
     3481 taggaaggtc tgggcggcaa gcgagtcctc cggaagccgg gcggggtcca gggtccggca
     3541 gatttcccct agcatcaggc ggaaatccgg ggccgtgacg gctacccacg ctacggcccg
     3601 ctccggattt tcctcgatcc gctccaccgc aaaggaaaca tggcgcccca ctaattcggc
     3661 aatgagcccg ctccccagtg agttggcctc cttgtattcc gccgggctac agacggcccg
     3721 ggcggcggcg gacagataac aataggcttc ctcggtgcgg ccgcggcggc gtgcgtcgag
     3781 atagttgacc aacgcctccc tggccgtcag ggtcgccccc ttacgaacca accagaggat
     3841 gaaaatcccg ccggtgagca acagggcaag gaggggaatc attgcttccg cacccgccgg
     3901 aggttatccc tccggctcga attgatcctt gccggctcca cagaccgggc aggtccaatc
     3961 cgccggcagg tcttcaaaag cggttcccgc cgggacatcg tgctccgggt cccccaggac
     4021 gggatcatag acatagccgc agatggagca tatatgacgg gacgccgtgg tcaccgccgc
     4081 ggccggcgga tcttcccttt cataggtcgg cgccgttttc ggcgccttgc ccttccggac
     4141 ggtatggtaa taggcgtagg tcatgggctc ttcgttgccg aggctgtcgt aatccaccac
     4201 ctcgccgacg aagatggtat gggaaccaca ttccagctct tggatcacct ttgcctccag
     4261 ataggccacg gcatgatcca ggacgatggg ggcgccggtt ttgcctgtct tgtacttgac
     4321 gttcttgaac ttatccgtat cccgtcccga tttgaagccg aacaggccga taaacgtcat
     4381 gggcgtctcc tcggcgagga tggataccga gaaaacccgg ctggaggtaa tataggcatg
     4441 ggtgaggttt tccttgttga tgctcacggc gatggtggaa ggttccgagg tgacctggaa
     4501 catgctgttg gcgatttgac cgttgaactt gccgtcctga acggaactga ctacgtacag
     4561 gccgtaactt atctt
//
LOCUS       DAOSMF010000142         4571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_20550_length_4571_cov_33.264836, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000142 DAOSMF010000000
VERSION     DAOSMF010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4571
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20550_length_4571_cov_33.264836"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1314
                     /locus_tag="PLT63_09930"
     CDS             <1..1314
                     /locus_tag="PLT63_09930"
                     /inference="COORDINATES: protein motif:HMM:NF013791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HOD99080.1"
                     /translation="LMDALALSRIQFAVTAMFHFLFVPLTLGLSILVAYMESRYVATR
                     DEMYLRMTKFWGKLFLINFALGVATGLSLQFQLGMNWAEYARYVGDIFAVPLAIEALL
                     AFFLEATFIGLWVFGWNKVSPRLHALSIWIVAIATNMSALWILLANGWMQHPVGFAIR
                     NNRAELVDFMAILTSGYGWLKFFHTILAGYVVAAFFVLGISAWHILRKNHLPLFKKSF
                     RIAATFGLASSLLIVLVGDFHGAEVGKAQPAKLAAMESLWETERGVPFYLLLVPDPTN
                     ERNAVELLGIPKMVSFLAFKDFNAEVKGLKAFPKEERPPVWETFLGFRLMVALGGLFI
                     LLSALAFFLSRKEALERQPLLLKALILALPLPYLAGELGWIVAEVGRQPWIVYGVLKT
                     SEAVSRGVSTFQAAVSLAGFTLLYSILWGINIYLLAKYARKGPEV"
     gene            1319..2347
                     /gene="cydB"
                     /locus_tag="PLT63_09935"
     CDS             1319..2347
                     /gene="cydB"
                     /locus_tag="PLT63_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HOD99081.1"
                     /translation="MDYQSLWFALWAVLWAIYFFLDGFDLGVGILHPFLPRNDLEKRL
                     AMNSIGPVWDGNKVWLVTAAGVTFAAFPAAFALIFSAFYLPLLLILFGLIVRGAALEF
                     RGKSDSPAWRRGWDMAFFLGSLTTAFLFGVLFGNIFQGLPLNAQGYQGGFFSLFNRYG
                     LLTGVMFVVMLGGHGALWLAVKTGDELGARVRRFAMKIWAVLVAATALFFVNTAFVTS
                     IFASYINNYVLLLLPAGVVASLLGMGMAIMSKRFPAAFFASGAAVILIVMTGFAGLYP
                     QLVPARPDALYSLTIYNAAASDYTLRILTVVAAIFVPVVAVYQFWVYRIFRGPVTGED
                     LRDDKEAY"
     gene            2367..3947
                     /locus_tag="PLT63_09940"
     CDS             2367..3947
                     /locus_tag="PLT63_09940"
                     /inference="COORDINATES: protein motif:HMM:NF025052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOD99082.1"
                     /translation="MTHPRLVAAMSDPGFYPHRPAGVEMVQTHISYVFIAGDEVYKVK
                     KAVDFGFLDFTTLEKRRHYCHEELRLNRRLAPETYLEVATIREEGRDGRLVLGADGGA
                     GGRVVDYAVRMRKLPPERMLKRLIVARDFDPAVFTAIADKLAAFHRQAETGGRIDEIG
                     SPETIRHNHEENFAQTEKYIGMTIPDFQFRFIRNYIFRFMEENAALLARRVREHRIRD
                     GHGDLHLEHIVVDREIVIFDCIEFNERFRYSDVAAEAAFLAMDLDYNGYEDHADRFVT
                     DYARLAGDGELLGLINFYKCYYAYVRGKVVSFRLDDPAIDVRGKEEARQLAVRYFDLA
                     YTYAARLEQPALILITGLMGTGKSVLASLAAPRLGAAVIRTDVLRKELLNLAPTDRHH
                     VEFGQGIYGEDISEQTYAAAHERAAELLQSGRPVIIDASYKRARQRNDALALARRLGV
                     PFLLVECVCPDEAVRERLERRARDPREASDGRWEIYAAQKGDYDREDGLPEDNRLTVD
                     SSRPPETCLHRTLARLRS"
     gene            complement(3880..>4571)
                     /locus_tag="PLT63_09945"
     CDS             complement(3880..>4571)
                     /locus_tag="PLT63_09945"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HOD99083.1"
                     /translation="LWSRKQMAKGTPEALYSAGVAAYQDGRYKRAVEYFTRLKEENPL
                     HPLALQAEIGIGDAHFSDDEFAEAELAYTDFVSLHPTNENNPYVIYQIGMCHYKQMSS
                     IDRDQSETVKAAREFERVIARYPSSKFAFLAEQKLRECRQQLAEREFYIGEFYFKQGK
                     YEAALKRFQTVAKIYPHIGLDYKAKAYIAETQKRILKAEQKKASSKGISSGAGPVSGA
                     DMSPGAGRNQQ"
BASE COUNT          766 a         1406 c         1398 g         1001 t
ORIGIN      
        1 cttatggacg ccctggccct gagccggatc cagttcgccg tgacggcgat gtttcatttt
       61 ctcttcgtac ccctcaccct gggcctttcc atcctcgtgg cctacatgga gagccgctac
      121 gtggccaccc gggacgagat gtatctgcgg atgacgaagt tttggggcaa gctcttcctc
      181 atcaacttcg ccctgggggt ggcgacgggt ctttccctcc agttccaact gggcatgaac
      241 tgggcggagt atgcacgcta cgtcggcgat atcttcgccg tgccgctggc catcgaggcg
      301 ctgcttgcct ttttcctcga ggcgaccttc atcggcctgt gggtgtttgg gtggaataag
      361 gtatcccccc gcctccacgc cctgtccatc tggatcgtcg ccatcgcgac gaatatgtcg
      421 gccctctgga tcctcctcgc caacggctgg atgcagcacc ccgtgggctt cgccatccgg
      481 aacaatcgcg ccgaactggt cgatttcatg gcaatcctca ccagcggcta cgggtggttg
      541 aaattcttcc ataccatcct cgccggttat gtcgtggcgg cctttttcgt cctggggatc
      601 tccgcctggc atatcctcag aaagaaccat ctgcccctct tcaaaaaatc cttccggatc
      661 gcggcgacct tcggcctggc aagctcactc ctcatcgtcc ttgtcggcga cttccacggg
      721 gccgaggtgg ggaaggccca gccggccaaa ctggcggcta tggaatccct ctgggagacc
      781 gagcggggcg tgccctttta cctgcttctg gtccccgatc cgaccaacga acgcaatgcc
      841 gtggaactgc tcggcatccc caagatggtg agcttcctgg ccttcaagga tttcaacgcc
      901 gaagtgaagg gcctcaaggc ctttcccaag gaggagcggc cgccggtgtg ggagaccttc
      961 ctgggattcc gcctcatggt ggccctgggg gggctcttca tcctgctgag cgccctggcc
     1021 ttcttcctgt cccggaagga ggcgctggaa agacaacccc tcttgctcaa ggcgctgatt
     1081 ctggccctgc cgctgccgta tctggccggg gagctgggat ggatcgtcgc ggaggtgggg
     1141 cggcagccct ggatcgtcta tggggtttta aagacctcgg aggccgtttc ccggggcgtc
     1201 tcgacgtttc aggcggcggt gtctctcgcc gggttcaccc tcctttacag cattctctgg
     1261 gggataaaca tctatctgct ggcaaaatac gccagaaaag gaccggaggt ttgagctgat
     1321 ggattatcaa tcgctgtggt ttgccctctg ggcggtcctc tgggccatat atttcttcct
     1381 ggacggcttc gatctgggcg tcggcatcct ccatcccttc ctgccccgca acgacctcga
     1441 aaagcgccta gccatgaaca gtatcggccc cgtttgggac ggcaacaagg tctggctggt
     1501 gacggcggcc ggggttacct ttgccgcctt tcccgcggcc tttgccctga tcttcagcgc
     1561 cttttatctg ccgttgcttt tgatcctctt cggattgatc gtccggggcg ccgctctgga
     1621 attccgcggc aagagcgatt cccccgcctg gcggcgcgga tgggatatgg ccttttttct
     1681 cgggagtctg actacggcct ttcttttcgg ggtccttttc ggcaatatct ttcagggact
     1741 gcccctgaac gcccagggct accagggggg cttcttttcc ctgttcaacc gctacggact
     1801 tttgaccggc gtcatgttcg tcgtcatgct cggcggtcac ggcgccctct ggctggccgt
     1861 caagaccggg gacgaactgg gggcgagggt gcggcgattc gccatgaaga tctgggcggt
     1921 tctggtggcg gcgacggcgc tgttcttcgt gaacaccgcc ttcgtcacat ccatttttgc
     1981 cagttatatc aataattatg tcctgttgct ccttcccgcc ggggtggtgg cctccctgtt
     2041 ggggatgggc atggccatca tgagcaagag gtttcccgcc gcctttttcg cttccggggc
     2101 cgccgtcatc ctgatcgtca tgacaggatt cgccggtctt tatccccaac tggtcccggc
     2161 gcgtcccgat gccctttaca gcctcacgat ctacaatgcc gcggccagcg attataccct
     2221 caggatcctc accgtcgtcg ccgcgatctt cgtccccgtg gtggcggttt accaattctg
     2281 ggtttaccgg atattccgcg ggcccgtaac cggggaagac ctccgggacg acaaggaggc
     2341 ctactgatca tgaaggagaa tcttccatga cccatccgcg gcttgtcgcg gccatgagcg
     2401 atccgggatt ctatcctcac cgtccggcgg gggtggagat ggtgcagacc cacatctcct
     2461 atgtcttcat cgccggcgat gaagtttaca aggtgaagaa ggccgtggat ttcggattcc
     2521 tcgattttac caccttggaa aagcgccggc attactgcca cgaggagctg cgcctgaacc
     2581 ggcggctggc cccggagacc tatctggagg tggcgacgat ccgggaagag ggtcgggacg
     2641 gccgtctggt cctgggggcc gacggcggcg ccgggggacg ggtggtggat tacgccgtcc
     2701 ggatgcgcaa actcccgccg gagcggatgt tgaagcgcct catcgtcgcc cgggacttcg
     2761 acccggcggt tttcacggcg attgcggata agcttgccgc cttccaccgg caggccgaaa
     2821 cggggggccg catcgacgag atcggctctc cggagacgat ccgccacaat cacgaagaga
     2881 atttcgccca gacggagaaa tacatcggca tgaccattcc tgactttcag ttccggttca
     2941 tcaggaacta tatcttccgg tttatggagg aaaacgccgc cctcctggcc agacgggtcc
     3001 gggaacaccg catccgcgac ggtcacggcg atctgcacct ggagcacatc gtcgtggacc
     3061 gcgagattgt cattttcgat tgcatcgaat tcaacgagcg tttccgctac agcgacgtgg
     3121 cggcggaggc ggcctttctc gccatggatc tcgattacaa cggctatgag gaccacgccg
     3181 accgctttgt gacggattac gcccgcctgg ccggggacgg ggaactcctg gggctgatca
     3241 atttctacaa gtgctattac gcctatgtcc ggggcaaggt ggtaagcttc cggttggacg
     3301 atcccgccat cgacgtccgg ggaaaggaag aggcgcggca gctcgccgtg cgctatttcg
     3361 atctggccta cacctacgcg gcccgcctgg aacaaccggc gctgatcctc atcaccgggc
     3421 tgatggggac ggggaagagc gtcctggcct ccctggcggc gccccgcctg ggggcggcgg
     3481 tgatccgcac cgatgtcctc cgcaaggaac tcctgaacct ggcgcccacg gaccggcatc
     3541 atgtggaatt cggacagggg atttacgggg aagacatttc ggagcagact tacgccgccg
     3601 cccatgaacg ggcggcggaa ctgctgcaat cagggcggcc cgtcatcatc gacgcatctt
     3661 acaaacgggc ccgccagcgt aacgacgccc tggcgctggc caggcggctc ggggttccct
     3721 tcctgctggt cgaatgcgtc tgccccgacg aggcggtccg ggaaaggctg gagcggcggg
     3781 cgcgggaccc gcgggaggcc tcggatggcc gatgggagat ctacgccgcc cagaaaggag
     3841 actatgaccg ggaggacggc ctgccggagg acaaccggct tactgttgat tcctcccggc
     3901 ccccggagac atgtctgcac cggacactgg cccggctccg gagctgatcc ccttcgacga
     3961 agcctttttc tgctccgcct tcaagatgcg tttctgcgtt tcggcgatat aggctttggc
     4021 cttgtagtcg agaccaatgt gggggtagat tttggccacg gtctgaaaac gcttcaaggc
     4081 cgcctcgtat tttccctgtt tgaagtaaaa ctcaccgata taaaactccc gttccgccaa
     4141 ctgctggcgg cattcccgga gcttctgctc tgccaggaag gcgaacttgc tgctcgggta
     4201 gcgggctatg acgcgttcga actcccgggc ggccttgacg gtttcggact ggtcccgatc
     4261 gatggaggac atctgtttgt agtggcacat accgatctga tagatgacgt aggggttgtt
     4321 ttcgttggtc gggtgaagac ttacgaagtc cgtataggcc agttccgctt ccgcgaattc
     4381 atcgtcggaa aaatgggcgt ccccgatgcc gatttccgcc tgcagggcca gggggtgcag
     4441 ggggttctcc tccttgaggc gggtgaagta ctccacggcc cgcttgtacc gaccgtcctg
     4501 gtaagccgcc acgcccgccg aatacaaggc ttccggggtg cctttggcca tctgcttgcg
     4561 cgaccacaag t
//
LOCUS       DAOSMF010000143         4540 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_20713_length_4540_cov_27.208027, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000143 DAOSMF010000000
VERSION     DAOSMF010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4540
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20713_length_4540_cov_27.208027"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2800)
                     /locus_tag="PLT63_09950"
     CDS             complement(<1..2800)
                     /locus_tag="PLT63_09950"
                     /inference="COORDINATES: protein motif:HMM:NF018357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1156 domain-containing protein"
                     /protein_id="HOD99084.1"
                     /translation="MYPEAGNKKGAMQDLTFIETQFPVSLLSKESYKERKSNNSQTLT
                     GLGKWWGRKPLVLIRAALLGLLLPASTDPKQDREIFFKLLTMDEEGLWNRLRGNIPAA
                     DVYELATETEREFYFIREDGKWKWKRSATREDREHMQHRAFNRMGYDRKLSYCVRPEE
                     IAGPSEAAWQTINAHLGTETHSLPELVHELGKRRFGHTPRVGDAFCGGGSVPFEAAVL
                     GCDVYGADLNPVGALLTWAAIHLIGGGLKMAGGIKKVQKQVYEAVGRQIEAWGIERND
                     EGWQDEATGEIKGKGWQADYYLYCIEATDPATGWRVPLAPSWIIGQKTRTIARLVPEK
                     ATKSFRIEIVQDASPEEMEKAKLEGTAADGIRCPVDRSGNPIQPEMRTATPIDALRGR
                     EGLRRWENEDLVPRPDDVLQERLYCIRWVDPETGEKQYRAPTEADLQREAEALRLLKE
                     RFGAWQAKGFIPSRKIESGYNTDQPIRERGWTHWHHLYNPRQLLLIGLFQELAAETAK
                     TPEVGAALLLGIGRMADWTSRLCRWDSSAANEKGAQTFFNQALNTLANYATRAYNALK
                     TTWPLTFPERNVGSDSQVIPLDARLTAYAADLWITDPPYADAVNYEELSELFLAWYEK
                     RLPVLFPAWYGDSKRALAVKGADENFRIAMVECYRRLAEQMPDNGLQLVMFTHQDVDV
                     WADLALILWSAGLRVTAAWTIATETDTSFRAGKYVQGTVLLVLRKRTGSLRGDRSDIY
                     PDVQAEVQRQLKTMLEIDDKENPNFGDSDYQLAAYAAALRVVTAYSAIEDIDVERELR
                     RVRQAGESSPLTDMIRSAVRIASDFLVPDGLEPAVWKRLLPEERFYIKGVEIEAHGEY
                     RDGVYQEFARGFGIRDYRDLLGSGAANLTRLKTPGELKGRDLAGVGFAGSLLRQILFA
                     VYKTAEEDDPRPGLDYLKQ"
     gene            complement(2818..3504)
                     /locus_tag="PLT63_09955"
     CDS             complement(2818..3504)
                     /locus_tag="PLT63_09955"
                     /inference="COORDINATES: protein motif:HMM:NF024049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3780 domain-containing protein"
                     /protein_id="HOD99085.1"
                     /translation="MMVRRKRQFVGFGFDPGESAHHFAVILPEREEDAVTVEERFAWH
                     DGSDAIEGAGRSLVVKVFLDRYRWDRIADAARGQFNARIRANGHRSVAWKKGQPNILP
                     PHFGKELTLLAWAVEEMDESLIPNVIANWTGLEPEERWWLYTTVNATFTRAEAGKDRG
                     WRKAIKIAFSENPVPEMPGERFVEEPDPIPLPRKESAPKAEAAGSSAVSKRKKKTTSR
                     KTPQGSLFDE"
     gene            complement(3504..>4540)
                     /locus_tag="PLT63_09960"
     CDS             complement(3504..>4540)
                     /locus_tag="PLT63_09960"
                     /inference="COORDINATES: protein motif:HMM:NF024686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HOD99086.1"
                     /translation="NFRNRVESKLWPENTKEVAWSDIRQRAASDPSWVWHHPDALDQL
                     KDELVKRDLWREMMGYITRGPFEKPVTSVQIQVLNRDSESGLATLRIRPINGDTVFME
                     TKSVATTSSRKLEDYDIKTKALKLSFLCVDSTNEHTTGEPVAWANTIIVKHRFYQEGT
                     KRKCELKALPAGRIRYTTDGAGVETSGVPYDKPFEIPSECRVVLAVAEGDGIKSPAVN
                     IPAPQGKVDPAAALDRAKAAVWRRGFKKDATGDTYRFLETAKKHGAELGGARLTIAKD
                     ARWIELNTPDEEFHAVERFERGADLLKEFIPEGVLSIDVAGMKFDSGQQLLDMVADLK
                     TELKEGEVRQ"
BASE COUNT          780 a         1401 c         1389 g          970 t
ORIGIN      
        1 cctgcttcaa ataatccagg ccggggcggg gatcgtcctc ctcggcggtc ttgtaaacgg
       61 caaagaggat ctggcgcagg agcgatccgg caaagccaac gcctgcaagg tcacgcccct
      121 tgagttcacc gggcgtcttc aggcgggtca ggttagcggc gccgcttccg agcagatccc
      181 ggtagtcgcg gatgccgaag ccgcgggcga actcctgata aacgccgtcg cggtattcac
      241 cgtgggcctc aatctcaacg cctttgatgt aaaagcgctc ctccggcagg aggcgcttcc
      301 agacggctgg ctccaagccg tcggggacca gaaagtcgga ggcgattcga acggcgctcc
      361 tgatcatgtc cgtcagcggc gacgactcgc cggcctgccg gacacgccgg agctcccgtt
      421 ccacgtcgat gtcctcgatg gcgctgtagg cggtgacaac ccgcagggcc gcagcgtatg
      481 ccgccaactg gtagtccgag tcgccgaaat tcggattttc cttatcgtcg atctccagca
      541 tggtcttgag ttgtcgctgg acctcggcct gaacgtccgg gtagatgtcg gagcggtcac
      601 ccctgagcga gcccgtgcgt ttgcggagaa cgagcaggac ggtgccctgg acgtatttcc
      661 ccgcccggaa ggaggtgtcc gtctccgtgg cgatggtcca ggccgccgtc accctgagac
      721 ccgccgacca gaggatgagg gccagatccg cccagacatc cacgtcctga tgggtaaaca
      781 tgaccagttg caggccgttg tccggcatct gctcggccag ccgccggtag cactccacca
      841 tggcgatccg gaagttttca tcggccccct tgacggccag ggcgcgcttg ctgtcgccat
      901 accatgcggg aaagaggacg ggcaggcgct tttcgtacca ggccaggaag agttccgaga
      961 gctcctcgta gttgaccgcg tcggcataag gggggtcggt gatccagagg tcagcggcat
     1021 aggctgtcaa gcgggcatcc aggggaatga cttgggaatc ggaaccaacg ttcctttcag
     1081 gaaaggtgag cggccacgtc gtcttcagag cgttgtacgc tcgggtcgca tagttggcca
     1141 gggtgttcaa tgcctgattg aagaaggttt gagccccttt ttcatttgcc gcgctggaat
     1201 cccaacggca gagccttgaa gtccagtccg ccatccgtcc gatgccgagg agcaaggccg
     1261 cgccgacctc cggggtcttg gccgtctcgg ccgccagttc ttgaaaaaga ccgattaaca
     1321 acaactgccg gggattgtag aggtggtgcc agtgggtcca gccgcgttca cgaatcggct
     1381 gatccgtatt gtaacctgat tcaattttgc ggctggggat gaaccccttg gcctgccagg
     1441 ccccgaagcg ctctttgagg aggcgcaggg cctcggcctc ccgttgcaga tcggcctcgg
     1501 tcggcgcccg gtattgtttc tctcctgtct ccgggtccac ccagcggatg cagtagaggc
     1561 gctcctggag cacatcgtcc ggccgcggca ccaggtcctc gttttcccaa cgccgcagac
     1621 cctcgcggcc ccggagggcg tcgatggggg tggcggtccg catctccggc tggattggat
     1681 ttcccgacct gtccaccggg cagcggatgc cgtccgcggc tgtcccttcc agcttggcct
     1741 tttccatctc ctcgggactc gcatcctgga cgatctcgat ccggaaggat ttggttgcct
     1801 tttccgggac caggcgggca atggtccgcg tcttctggcc gatgatccag gagggggcga
     1861 gtggcacccg ccagccggtt gcggggtcgg tcgcctcgat gcagtagagg taatagtcgg
     1921 cctgccaacc cttgcctttg atttcgcccg tggcctcgtc ctgccagccc tcgtcgttcc
     1981 gttcgattcc ccaggcctcg atctgacgcc ccacggcctc gtaaacctgc ttctggactt
     2041 ttttgatccc cccagccatt ttcaagccgc cgccaatgag gtggatggcc gcccaggtca
     2101 gaagcgcgcc gacagggttc aggtccgcgc cgtaaacgtc gcagcccaga accgcggcct
     2161 cgaaggggac gctgccgccg ccgcagaagg cgtcgccgac gcggggagtg tggccgaagc
     2221 gcctcttgcc cagttcatgg accagttccg ggaggctgtg cgtctccgtt cccagatgcg
     2281 cgttgatcgt ctgccaggcc gcctccgagg ggccggcgat ctcttcgggg cgcacgcaat
     2341 aggaaagctt ccggtcgtag cccatgcggt tgaaggcccg gtgctgcatg tgttcccgat
     2401 cttcccgggt cgccgaccgc ttccacttcc attttccatc ctcgcggatg aaatagaact
     2461 ccctttccgt ctctgtggcg agttcataga catcggcggc ggggatgttc ccccgcaaac
     2521 ggttccagag cccctcctcg tccatggtca ggagtttgaa gaaaatctcg cggtcctgtt
     2581 tcggatcagt ggaggcaggc aggagcaatc ccaggagggc cgcccggatc aggaccaggg
     2641 gcttgcggcc ccaccacttg cccaagcccg tgagggtctg gctgttgttg gacttgcgct
     2701 ccttgtagct ttccttggaa agcaggctta cgggaaattg ggtttcgatg aaggttaaat
     2761 cctgcatggc gcctttcttg ttgccggctt cggggtacat gtcatacccc gtctctttta
     2821 ctcatcgaag agagagccct ggggggtttt cctggacgta gttttcttct ttcttttcga
     2881 aacggcggaa gatccggccg cttccgcctt tggggcgctc tctttccggg gcaaggggat
     2941 ggggtcgggt tcttccacga acctctcacc cggcatctcc ggaaccgggt tttcggaaaa
     3001 ggcaatcttg atcgccttgc gccagccccg gtccttccca gcctccgccc tggtgaaggt
     3061 tgcattgacg gtcgtataga gccaccagcg ctcctccggt tccagaccgg tccagttggc
     3121 gatgacgttg gggatgaggc tctcgtccat ctcttccact gcccaggcca ggagagtcag
     3181 ctccttgccg aaatgggggg gcaggatgtt gggctgaccc tttttccagg caacggagcg
     3241 atggccgttt gccctgatcc tcgcgttgaa ctgacctctg gccgcatccg cgattcgatc
     3301 ccagcggtag cggtccagga aaactttgac aacgagagaa cggcctgcgc cctcgatggc
     3361 atcggaccca tcatgccagg cgaagcgttc ttcgacagtc acggcgtctt cttctctttc
     3421 cgggaggatg acggcaaaat ggtgagccga ctcaccggga tcgaaaccga agccgacaaa
     3481 ctgacgtttt ctgcggacca tcatcactgg cgcacttccc cttctttgag ttccgtcttc
     3541 agatcggcaa ccatgtcgag aagctgctgc cccgaatcga acttcatgcc ggcaacatcg
     3601 atgctcaaaa ccccttccgg aatgaattct ttgagcaggt cggcgccgcg ctcaaagcgc
     3661 tctacggcgt gaaactcttc atccggagtg ttgagctcga tccagcgggc atccttggcg
     3721 atggtcagac gtgcgccgcc caactccgcg ccgtgcttct tggccgtctc caggaaccgg
     3781 taggtgtctc ccgtggcgtc cttcttgaac ccgcgtcgcc agacagcggc tttggcacgg
     3841 tcaagggccg cagcgggatc gactttgccc tggggcgccg gaatattgac tgccggggat
     3901 ttaatcccat ctccctcggc aacggcaagg acaacccggc actccgacgg aatttcaaag
     3961 ggcttgtcgt agggcacacc ggatgtctca acgcccgcgc cgtcggtggt gtatcggatt
     4021 cggcctgcag gcagggcttt gagttcacat ttgcgcttcg ttccctcctg gtaaaagcgg
     4081 tgcttgacga taatcgtatt ggcccaggca accggctcgc ccgtggtatg ctcgttcgtg
     4141 gagtcaacgc agaggaaaga gagtttcagt gccttggtct tgatgtcgta gtcttccagt
     4201 tttctggaag aggttgtggc aacacttttc gtttccatga agaccgtatc gccgttaatg
     4261 ggccggatgc gcagcgtcgc caaacctgat tcggaatcgc ggttcaaaac ctgaatctgg
     4321 acggaggtga ccggcttttc aaagggaccc cgcgtgatgt accccatcat ctcccgccag
     4381 agatcccttt tgaccagttc gtccttcagt tggtcgaggg cgtcggggtg gtgccagacc
     4441 caggaagggt cggaggcggc ccgctgacgg atgtcgctcc aggcaacctc cttggtgttt
     4501 tccggccaga gcttgctttc cacccggttc cggaaatttc
//
LOCUS       DAOSMF010000144         4428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_21420_length_4428_cov_25.081637, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000144 DAOSMF010000000
VERSION     DAOSMF010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4428
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21420_length_4428_cov_25.081637"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /gene="leuB"
                     /locus_tag="PLT63_09965"
     CDS             complement(<1..773)
                     /gene="leuB"
                     /locus_tag="PLT63_09965"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HOD99087.1"
                     /translation="MEKKNKIAVFPGDGIGVEVVREAVKVLDALAEKTGAGFALAYGL
                     VGGAAYDACGDPFPAESLELALTSDVVLLGAVGGPKWEGLDYSVRPERALLGLREKLN
                     LFANLRPVNVYDELIEASPLKAEIVRGIDILIIRELTGDLYFGQPRGVRVENGRRVGI
                     NTLVYTEEEIRRIARVAFAAAQQRRKRLLSVDKANVLESTQLWRDVVTEEGRAYPDVE
                     LRHMYVDNCAMQLVSHPAQFDVIVTTNMFGDILSDEAAM"
     gene            complement(798..1289)
                     /locus_tag="PLT63_09970"
     CDS             complement(798..1289)
                     /locus_tag="PLT63_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOD99088.1"
                     /translation="MRFKGKVWKFGDHVNTDLIIPARYINSSDPDFLAAHCMEDVDPD
                     FVKKLRPGDIIVAGENFGCGSSREHASIAIKAAGVAGVVARNFARIFYRSAFNTGLPI
                     FESATLYDAVQEGDEIEVDGDAGVITVTGKDPRQLKIEPIPPFMQELIAAGGLMAYLK
                     KRP"
     gene            complement(1286..2557)
                     /gene="leuC"
                     /locus_tag="PLT63_09975"
     CDS             complement(1286..2557)
                     /gene="leuC"
                     /locus_tag="PLT63_09975"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOD99089.1"
                     /translation="MTITEKIMAAHADLEEVHPGMLINARVDIALGHDVTAPIAIAEF
                     KKAGGRKVFDRGRVVLVADHFTPTKDIKSAQQVKIMRDFAREHRLGHYYEGGRAGVCH
                     VLLPEQGIALPGDLIIGADSHTCTYGALGAFATGVGSTDLTAVMLTGELWFKVPETIK
                     IVLTGRCNKWVSGKDIILYIIGQIGVDGATYKAMEFTGETVGELTMADRFTMANMVIE
                     AGAKNGIFIPDAVTTEYVQYRAEREYASYDSDPDARYASVLEIDVSRIEPQVAFPHLP
                     SNVKGLSRVGSIKIDQVFIGSCTNGRLEDLRIAANLLQGREVAPYVRLIVIPTTPDIY
                     RTAMSEGLFDIFLAAGAVISPPTCGACLGGHMGILADGERAVATTNRNFIGRMGHPGS
                     EVYLTNPAVAAASAILGRIAGPDELKGTHKI"
     gene            2781..3812
                     /locus_tag="PLT63_09980"
     CDS             2781..3812
                     /locus_tag="PLT63_09980"
                     /inference="COORDINATES: protein motif:HMM:NF017847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HOD99090.1"
                     /translation="MEMGILGLPQSGKSSLFEIMTGIRSRDIHGERCVRGQAAVPDAR
                     FDRLVDIFHPAKISPARAPFVDVHADGEKAWEAVRQTLAGADGLLHVVDGFTAADLQE
                     TVARWRTLEDELILSDLMVVENRRERLQRVPRKALKPVELLQAELLPRLHDQLEQGRP
                     LRDLPLSAEERHALRSFSFWSLRPELVVLNLSEDRLGETAAFRQEAGLADHVIGFCGE
                     LEAEMAGLSREEQQEFLESLAMAEPAFQRIIQAAFRLLGRITYFTVGEDEVKAWVIPR
                     GVKAPRAAAAIHKDFERGFIKAEVVSYDDFVACGGILAAAKAAGKLRLEGKDYVVADG
                     DIISFRFNV"
     gene            3860..4360
                     /locus_tag="PLT63_09985"
     CDS             3860..4360
                     /locus_tag="PLT63_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99091.1"
                     /translation="MANDERYDAMTGENRLIPILREGIHVIKMVLFKTLRDGLAERHP
                     RRERIFINQLAGAVINDLFGTPNRDEPFLSFGIANRALIEMELAGLAGDYPELRAPLS
                     DALRVQFLCDSREGLDNPALLTRADALGILIRDREVPLPDTFMNMARFLGEEHQMTLQ
                     IRLGSA"
BASE COUNT          818 a         1338 c         1374 g          898 t
ORIGIN      
        1 atcatggccg cctcgtcgct gaggatgtcg ccgaacatgt tggtcgtcac gatgacgtca
       61 aactgggcgg gatgggagac cagttgcatg gcgcagttgt ccacgtacat gtggcgcagc
      121 tccacgtcgg gataggcgcg cccttcttcc gtcaccacgt cccgccagag ctgggtgctt
      181 tccaggacgt tggccttgtc cacggagagg agacgcttcc gccgctgctg cgctgccgcg
      241 aaggccaccc gggcgatgcg ccggatctcc tcctcggtat agacaagcgt attgatcccg
      301 acgcgccggc cgttttccac ccggacgccc cggggctggc cgaagtagag gtccccggtg
      361 agttcccgga tgatgaggat gtcgatgcct cggacgatct ccgccttcag aggtgatgcc
      421 tcgataagtt catcatagac attcaccggc cgcaaattgg cgaatagatt gagtttttcc
      481 cgcaatccca ggagtgcccg ctccggccgg acactatagt ccagtccttc ccattttggt
      541 ccccccacgg cgccgagaag aacgacgtca ctcgtcaggg ccagctccag actttccgcc
      601 gggaaggggt cgccgcaggc gtcgtaggcc gcaccgccca ccaggccgta ggccagggcg
      661 aagccggccc cggtcttttc cgccagggca tccaggacct tcacggcctc ccggaccacc
      721 tccaccccga tgccgtcacc gggaaagacc gctattttat tctttttttc caatcaaaaa
      781 ctcctcaatc aggatggtca cgggcgcttt ttcaggtacg ccatcaaccc tccggcggcg
      841 atcagctcct gcatgaaggg ggggatgggt tcgattttga gctgccgcgg atcttttccc
      901 gtcacggtga tcacgcccgc gtcgccgtcc acctcgatct catcgccctc ctggacggca
      961 tcgtagagcg tcgccgactc gaagataggc agacccgtgt tgaaggcgct gcggtagaag
     1021 atccgggcga aattccgggc gacgacgccg gcgacgcccg ccgccttgat ggcgatggaa
     1081 gcatgttccc gggaggagcc gcagccgaag ttttcccccg ccacgatgat gtcgccgggg
     1141 cgcagcttct tcacgaaatc cgggtcgacg tcctccatgc agtgggcggc cagaaaatcc
     1201 gggtcggaac tgttgatgta acgggctggg atgatcaggt cggtattgac atgatcgccg
     1261 aatttccaga cttttccttt aaatctcata tcttgtgcgt ccccttgagt tcatcgggac
     1321 cggcgattct ccccaggatg gcggaggccg ccgccacggc cgggttggtc aggtaaacct
     1381 cgctgcccgg gtgtcccatg cggccgataa agttccggtt ggtggtggcc acggcccgct
     1441 cgccgtcggc gaggatgccc atgtgtcccc ccaggcaggc cccgcaggtc ggcgggctga
     1501 tgacggcccc ggcggccagg aagatgtcga agagcccctc ggacatggcg gtgcgataga
     1561 tgtccggagt cgtggggatg acgatgaggc gcacatacgg tgcaacctcc cggccttgca
     1621 gcagattggc ggcaatgcgc agatcttcga ggcggccgtt ggtgcaggaa ccgatgaaga
     1681 cctgatcgat cttgatggag ccgaccctgc tcaacccctt gacattggaa ggcaaatggg
     1741 gaaaggccac ctggggttcg atccgactga cgtcgatctc caggacggag gcgtaacggg
     1801 cgtcggggtc cgaatcgtaa ctggcgtatt cccgttccgc ccggtattgt acgtattcag
     1861 tggttacggc atctgggatg aagatgccgt ttttcgcccc ggcctcgatg accatgttgg
     1921 ccatggtgaa gcggtccgcc atggtcaact ctccgacggt ttccccggtg aattccatgg
     1981 ctttgtaggt ggcgccgtcc acgccgatct gcccgatgat atagagaatg atgtcctttc
     2041 ccgagaccca tttgttgcac cggcccgtca ggacgatctt gatcgtttcg ggaaccttga
     2101 accagagttc ccccgtcagc atgaccgccg tcagatccgt gctgcccacg ccggtggcaa
     2161 aggcgcccag ggcgccgtag gtgcaggtgt ggctgtcggc cccgatgatc agatcgccgg
     2221 ggagggcgat cccctgttcc ggcagtagga catggcacac cccggcccgg ccgccctcgt
     2281 aataatggcc cagccggtgt tcccgggcga aatcacgcat gatcttcacc tgttgggctg
     2341 attttatgtc cttggtcggg gtgaagtgat cggcgacaag aacgacccgg ccgcggtcaa
     2401 agaccttgcg tcccccggcc ttcttgaact ctgcgatggc aatgggcgcc gtcacgtcgt
     2461 gccccagtgc gatgtccacc cgggcgttga tcaacatccc cggatgaacc tcttcaaggt
     2521 ccgcatgggc ggccatgatc ttttcggtga tcgtcatccc catgaaacga ttctcctttt
     2581 tcttttcggt tgacggttgt aaaatatggt ccaactcata tgagtagttt ctacaatatt
     2641 atcgtttccg attcaatggc taaatccaaa ttttacgggc gccggcgggg aggtgatatt
     2701 ttgcttgaaa tggttccgcg gggatgatat ggggcgcagg ccttgcgaga ggcgaaaggc
     2761 aaatgaagcg gtagatagat atggaaatgg gtattttagg tcttccccaa agcgggaaga
     2821 gttcactttt cgagatcatg acgggtatcc gcagccggga catccatggc gaaagatgtg
     2881 tccgcggcca ggcagcggtt cccgatgcgc gcttcgaccg cctcgtggac atctttcatc
     2941 ccgccaagat ttctcccgcc cgggctccct tcgttgacgt acatgccgac ggcgagaagg
     3001 cctgggaggc ggtccgccag accctggctg gcgccgacgg cctcctgcat gtggtggatg
     3061 gatttactgc cgccgatctt caggagacgg tcgcccggtg gcggacgctg gaggatgaac
     3121 tgatcctctc cgacctcatg gtggtggaaa accgccggga gcgcctccaa cgagtccccc
     3181 ggaaggctct caaaccggtg gaactgctcc aggcggaact gctgccccgc ctccatgacc
     3241 agttggagca gggacgtccc ctccgggatc tgccgctcag cgccgaggag cgtcacgccc
     3301 tgcgcagttt ctccttctgg agcttgcggc cggaactggt agtcctaaac ctttccgaag
     3361 atcgtcttgg agagacggcg gccttccgtc aggaagccgg gcttgccgat catgtcatcg
     3421 ggttttgcgg cgaactggag gcggaaatgg caggcctgtc ccgggaggaa cagcaggagt
     3481 ttctggagtc cctggcgatg gccgaaccgg ccttccagcg gatcatccag gccgcttttc
     3541 gcctcttggg acggatcacc tacttcaccg tgggggaaga cgaggtgaag gcgtgggtga
     3601 tcccccgggg tgtcaaggcc cctcgggccg ccgcggccat tcacaaggac ttcgagcggg
     3661 gctttataaa ggcggaggtg gtgagctacg acgacttcgt cgcctgtggc gggatcctgg
     3721 ccgccgccaa ggccgcgggg aagctgcgcc tggagggcaa ggattacgtt gtcgcggacg
     3781 gagacatcat ctcgttccgc ttcaacgttt gacgaaattg ccaaaaagtc agcgcgggcg
     3841 gttaagaatc aggcaatatt tggcgaacga cgagaggtat gacgccatga ccggggaaaa
     3901 cagattgatc ccgatccttc gggaggggat tcacgtaatc aagatggtcc tcttcaagac
     3961 gctgcgggac gggctggcgg agcgccatcc acggcgggag cggatcttca tcaatcaact
     4021 cgccggtgcc gtaatcaacg atctattcgg cactccgaac cgggacgaac cgtttctctc
     4081 ctttgggatc gccaaccggg ccctgatcga aatggaactg gccggtctgg ccggggatta
     4141 tccggaactc cgggcccccc tgtcggacgc cctgcgggtc cagtttctct gcgacagccg
     4201 ggaaggcctg gacaatcccg ccctccttac ccgggccgac gccctaggca tcctcatccg
     4261 agaccgggag gttcccctgc ccgacacctt catgaacatg gcccgtttcc tgggcgaaga
     4321 acatcagatg actcttcaga tccgcttagg gtcggcctga gcgggtattt acggcaacgc
     4381 ttccagggcc gcgattcgct cctccaaagg cggatgggtc atgaatag
//
LOCUS       DAOSMF010000145         4410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_21538_length_4410_cov_19.859242, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000145 DAOSMF010000000
VERSION     DAOSMF010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4410
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21538_length_4410_cov_19.859242"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="PLT63_09990"
     CDS             complement(<1..685)
                     /locus_tag="PLT63_09990"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOD99092.1"
                     /translation="MTAAGAVERIAARIEAARGETAPDLVLKNGLVADMFTGEFVRTD
                     VALFDGVIVGLGTYDGPRCLDAKDYYVMPGFIDGHFHLESSMLSPRELARAVLPHGTT
                     AIIVDPHEITNVLGLRGLSYILQDSEGLPVDFYLMAPSCVPATHLETSGANLAAEDLA
                     ALRAERRILGLAEMMNFPGVIYKDPAVLAKLAAFHGAIVDGHAPLLSGKDLNAYVGAG
                     IRSDHECTQL"
     gene            complement(682..1203)
                     /locus_tag="PLT63_09995"
     CDS             complement(682..1203)
                     /locus_tag="PLT63_09995"
                     /inference="COORDINATES: protein motif:HMM:NF019500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HOD99093.1"
                     /translation="MNKRPAVAFGILFLTLVIDSAGCHLSEGFDYFKSLYLTIVTIST
                     VGYGDVYARSTGGMIFALVLIIAGVGTMFYTVSLFAETLVENRLQDIMGREKMKRMII
                     TMKKHNIICGCGRIGFLICRELAAGKVPFVVIEQDPAATYVIEKGENLIALGKDANVM
                     NFTKFCTAKEVTL"
     gene            complement(1200..2165)
                     /locus_tag="PLT63_10000"
     CDS             complement(1200..2165)
                     /locus_tag="PLT63_10000"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOD99094.1"
                     /translation="MKRAAADLPPPSKRVGLALGSGSARGWSHIGVIKGLQEAGIKIE
                     LVCGTSMGAMVAGAFAAGFIDVMDEWVRGLTWPDILGFMDVRLPRSGLIEGDKLTRYF
                     RENISDSHIEDLPMPYAAVTTELKSGREVWIREGSLMDAIRASMSMPGILTPFPGKEG
                     WFVDGGLVNPVPVSLCRTMGADIVIAVDLNNDIMGRSFNRRDRNGEQPLETEANGQYL
                     PNRLAAFFNGKLKTGQLSIFKHYAHNQPNRGPTLFEVIATSVYIMQDQITRHRLAADP
                     PEVLIRPRLAHIGLLEFNRAAEAIEEGRKAVALMLPILLDVLGTP"
     gene            2345..3025
                     /locus_tag="PLT63_10005"
     CDS             2345..3025
                     /locus_tag="PLT63_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HOD99095.1"
                     /translation="MNLFRGVIITTAVILTFSMSAEAAQDIPFSGFFGNPSIYDQLQP
                     GPKDGAKRRWIKSGVDPTKYQKFMVDSVVFFLADNADYKGIDPQEMKELCDAFNKEIA
                     AALKNKYPIVAEPGPDVARLSIAITNIRPSKPGVSAVTSIIPVGLGVSLVKKGATGGW
                     SGSGQTCMEIMVLDSATNEIIILAVDQRKAEFEDRFTKWGSAYDSFKFWSVKIVEFID
                     NSRKKDRK"
     gene            3066..>4410
                     /locus_tag="PLT63_10010"
     CDS             3066..>4410
                     /locus_tag="PLT63_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HOD99096.1"
                     /translation="MRHWIGHRDGIYHPPFLFLLFFLFVFCTSGSYAWAAKTIPGDYG
                     RPFSFADLAEKWGPAVVNISTTQTVKSGRGLYGNMPRHFFDQDDFFRRFFGDMPEREY
                     KQNSLGSGFLISSDGYIFTNNHVVAKADKIKVKLSNGKQYDAEVKGKDPNTDLALIKI
                     KADEKLPCVQLGDSDKLRVGDWVFAIGNPFGLDHTVTVGIVSAKGRVIGAGPYDNFIQ
                     TDASINPGNSGGPLFNLDGEVVGINTAIVAQGQGIGFAVPVNLAKDILKDLKEKGHVT
                     RGWLGLSIQDITTDMADNLKLQDRRGAIVGQVFPGDPADKAGIKTGDIIKAIDGRKIE
                     NTQELLRVVASLPVGKKVEVLIFRNGQELRKSLVVAERKDSREIASGKKINGRLGLTV
                     QEVTPEMARILKLTDKGGVVITRVDQDSPADDAGLKVNDVILQINRVRIQSLQDYT"
BASE COUNT         1049 a         1231 c         1198 g          932 t
ORIGIN      
        1 ccaattgcgt gcactcgtga tcggagcgga ttccggcacc gacataggcg ttgagatctt
       61 tccccgacaa gagcggggca tgaccatcca caatggcacc gtggaaggcg gccagtttgg
      121 ccaggacagc gggatcctta taaatgaccc ccggaaagtt catcatctcc gccagcccga
      181 gaatccttct ttccgctcgt aaggcagcca gatcttccgc cgccaggttc gctccggatg
      241 tttccaggtg tgtcgccggc acgcatgagg gcgccataag atagaaatca acgggcaatc
      301 cctcactatc ctgaaggatg taactaagac cccgaaggcc caggacgttt gtgatttcgt
      361 gagggtcgac aatgatggcc gttgttccgt ggggcaggac tgccctggcc aattcccggg
      421 gggaaagcat ggaactttcg agatggaaat gaccgtcgat gaatcccggc atcacataat
      481 aatccttggc atccaggcac cggggtccgt catatgtacc cagaccgact attacaccgt
      541 cgaagagggc cacatccgtg cggacaaatt caccggtaaa catatcggcc accaatccgt
      601 ttttcaggac caggtccggc gccgtctctc cccgggcggc ctcgatccgg gccgcgatcc
      661 tctccactgc cccggcggcg gtcatagtgt cacctccttt gccgtacaga acttggtaaa
      721 attcatcacg ttggcgtctt tccccaaggc gatcaaattt tcaccttttt cgatcacata
      781 agttgcggcg ggatcctgtt cgataaccac aaaggggacc tttcccgcgg ccaactcccg
      841 gcaaatcaag aatcctatac ggccgcatcc gcatattatg ttatgtttct tcatagttat
      901 gatcatcctt ttcatttttt cccggcccat gatatcctgc aagcggttct ccacgagggt
      961 ctcggcaaag agacttacgg tatagaacat cgttcccact ccggcgataa tcaagaccag
     1021 ggcaaaaatc atcccgccag tgctccgggc atatacatct ccataaccaa ccgtggagat
     1081 ggtcacgata gtcagatata atgacttgaa ataatcgaaa ccttcgctga ggtggcatcc
     1141 cgcggaatcg atcaccagcg tcaaaaagag aataccgaag gccaccgccg ggcgtttgtt
     1201 catggggttc ccaaaacatc gaggagaatg ggcagcatca aggccacggc cttgcgtcct
     1261 tcctcgatag cttccgccgc ccggttgaat tccagaagac cgatatgggc cagccggggg
     1321 cggatcagga cttccggggg atcagccgcc agtcgatgcc gggtgatctg gtcctgcatg
     1381 atatagaccg aggtggcaat gacctcgaag agggtcggcc cgcggtttgg ctggttatgg
     1441 gcgtaatgtt tgaaaatgga gagttgtccc gtcttgagtt tgccgttgaa aaaagccgcg
     1501 agccggttgg gaagatactg gccgttcgcc tccgtctcca gcggttgttc cccatttcga
     1561 tcccggcggt tgaaactccg gcccatgatg tcgttgttga gatcgacggc tatgacaatg
     1621 tcggcgccca tggtccggca gagggatacc ggcaccggat tgacgagacc gccgtcaaca
     1681 aaccagccct ccttcccggg aaaaggggtc agaatccccg gcatggacat gctggcccga
     1741 atggcgtcca ttagtgagcc ttcccggatc catacttccc gtccggactt cagctcggtg
     1801 gttactgcgg catagggcat gggtaggtct tcgatatggg aatcggagat gttttcccgg
     1861 aaataacggg taagcttgtc gccttcgatg aggccggaac gggggagccg gacatccatg
     1921 aagccaagga tatcaggcca ggttaaaccc cggacccatt cgtccatgac atcgatgaag
     1981 ccggcggcga aagccccggc caccatggca cccatggagg tgccgcagac cagctcgatc
     2041 tttatccccg cctcctgcaa tcccttgatc accccgatat gtgaccagcc ccgcgcggag
     2101 ccgctcccca aggcaagacc tactcgcttc gacggcggcg gtaaatcggc cgcggccctc
     2161 ttcatcaccg cttttttcat cccgacaccc ctctctgggt aagttttcct aacatacaaa
     2221 aaactacatg ggaaggagag ttttttaatt tcgttgttga aaagatccgc tggttgaaat
     2281 atagtctgcc tagcggatac atacaattcc atggctaata aacaagagtg taaggagaga
     2341 agatatgaat ttgttccgag gcgtaataat taccaccgcg gttatcctga ccttttccat
     2401 gtctgccgaa gccgctcaag acatcccctt ttcaggattt ttcggcaatc caagcatcta
     2461 cgatcaattg cagcccggcc ccaaagacgg cgccaaacga cgctggataa agtcaggggt
     2521 ggatccaacc aaataccaaa agttcatggt ggacagcgtc gtattctttc ttgccgacaa
     2581 tgccgattac aagggtatag atccccagga gatgaaggaa ctctgcgatg ccttcaataa
     2641 ggaaattgcc gccgccttga aaaacaaata ccccatcgtc gctgaacccg gcccggacgt
     2701 ggcgcggctc agtatcgcca tcaccaacat ccggccgagc aaacccgggg taagcgccgt
     2761 aacgtccata atcccggtgg gtctgggggt cagcctcgtg aagaaaggag ccaccggcgg
     2821 ctggtccggc agcggtcaga catgcatgga aataatggtc ctagacagcg cgacgaacga
     2881 gattatcatt ctggcggtcg atcaacggaa ggcggaattc gaagaccgct tcacgaagtg
     2941 gggttcggct tacgactcct tcaaattttg gtcggtaaag atagtggaat tcatcgacaa
     3001 ctcacgaaag aaagaccgaa aatagccctg acggtcttaa ccgctcgttg tgaaggagat
     3061 tggacatgag acattggata ggccatagag atgggatata tcatccaccc tttttgtttt
     3121 tactcttttt tttattcgtg ttttgcactt cgggcagtta tgcctgggcc gcaaaaacaa
     3181 tccccggtga ttacggccgg ccgttttcct tcgccgatct ggcggaaaaa tggggcccgg
     3241 cggtggtaaa tatcagcacg acccagaccg tcaagtccgg ccgaggactt tatggaaaca
     3301 tgccgcggca ttttttcgat caggatgatt tcttccgccg ctttttcgga gacatgccgg
     3361 agcgggaata caagcaaaac agtctgggct ccggttttct catctccagt gacggttaca
     3421 tcttcaccaa caaccacgtc gtcgccaaag ccgacaagat caaggtaaag ttatcgaacg
     3481 gaaagcaata tgatgcggag gtcaagggca aggatcccaa caccgatctc gccctgatca
     3541 agatcaaggc ggacgagaag ctgccctgcg tccagttagg tgattcggac aaactccggg
     3601 ttggcgattg ggtatttgcc atcggcaatc ctttcggcct cgatcatacg gttaccgtcg
     3661 gcattgtcag cgccaaggga cgggtcatcg gtgctgggcc ctacgacaat ttcatccaga
     3721 ccgacgcctc gatcaatcca ggcaacagtg gcggaccgct cttcaatctc gacggcgagg
     3781 tcgtgggcat caataccgcc atcgtcgccc agggacaggg catcggcttt gccgttcccg
     3841 tcaatctcgc caaagacatc ctcaaagatc tcaaggaaaa gggtcacgtc acccggggct
     3901 ggctgggtct gtccattcag gacatcacca cggacatggc ggacaatctg aagctccagg
     3961 accgtcgggg ggctatcgtc ggccaagtat ttccaggcga tcccgccgac aaggccggga
     4021 tcaaaaccgg tgacatcatc aaggccatcg acggccggaa aatcgaaaat acccaggaac
     4081 tgttgagggt cgtcgcctcc ctgccggtgg gcaagaaggt ggaggtcttg attttccgca
     4141 acggtcagga gctgcggaag tccctcgtgg tcgcggaaag aaaggattca cgggaaatcg
     4201 cctccggcaa aaagatcaat ggacggttgg gcctcacggt gcaggaagtc acgccggaga
     4261 tggcccggat cctgaaactg acggacaagg gcggcgtggt gatcacccgc gttgatcagg
     4321 attctccagc cgatgatgcg ggactgaagg taaatgacgt catcctgcag atcaaccggg
     4381 tgcggatcca atccttgcag gattacaccg
//
LOCUS       DAOSMF010000146         4374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_21768_length_4374_cov_23.717296, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000146 DAOSMF010000000
VERSION     DAOSMF010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21768_length_4374_cov_23.717296"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(191..1246)
                     /locus_tag="PLT63_10015"
     CDS             complement(191..1246)
                     /locus_tag="PLT63_10015"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HOD99097.1"
                     /translation="MANRTGVVVDERYLRHGEGYYHPESPRRLESLYAMLQHPGMEGK
                     YTIIAPRYATHEEIGLVHSPSYIGLVASTAGKSYYSLDADTQTSPESYDTAKLAVGGV
                     LNAVEAVMNGQVDNAFAFVRPPGHHAGKERGAGFCLFNNVAVGARYALGKYGLKRILI
                     ADWDLHHGDGTQEIFYRDKKVLYFSTHQYPAYPGSGTFTETGQGDGSGYTVNVPLKAR
                     TDDALHVKAFRGILQPVALKFQPELVLLSAGFDSYFQDPLGDMRVTPKGYVYMMRILL
                     DITAACCGGRLVAVLEGGYNVEGLTESVKLVLREMRGDTRASEDDLYRMEYEAEQGKD
                     QILRRVTEQINPVWQVF"
     gene            1399..1665
                     /gene="tatB"
                     /locus_tag="PLT63_10020"
     CDS             1399..1665
                     /gene="tatB"
                     /locus_tag="PLT63_10020"
                     /inference="COORDINATES: protein motif:HMM:TIGR01410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HOD99098.1"
                     /translation="MFGIGLPELIVIAVVALLVVGPEKLPGLAKSLGKGFQEFRRATD
                     DVTESIKDTLKPDEIKKDVDGLKDSLLYGGKEERDKDATPKNQS"
     gene            1693..2469
                     /gene="tatC"
                     /locus_tag="PLT63_10025"
     CDS             1693..2469
                     /gene="tatC"
                     /locus_tag="PLT63_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOD99099.1"
                     /translation="MGQTEDEKLPLTSHLAELRTRLYRILIAVGMGFFACYYFREGLF
                     RIITKPLTEVLPKNSHMIFTGMPEAFFIYMKIAFFASLLLTSPYTLYQLWKFVSPGLY
                     NSEKRYVVPFVLISTILFLGGVVFGYLLVLPTAFEFFITFSTDFLKPMISFREYLSFT
                     MKFLLAFGISFELPVFIFFLTKVGVVNAPLLSRYRRYAILMIFIAAAVLTPSPDAFSQ
                     ILMAVPLMFLYEISILVSRYAGRKKKAGEENAAAGVLDNE"
     assembly_gap    2586..2685
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2686..>4374
                     /locus_tag="PLT63_10030"
     CDS             <2686..>4374
                     /locus_tag="PLT63_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HOD99100.1"
                     /translation="IMVLALTGYLLYFFLGELPSVDTLKDYRPSIATRIYADNNELVD
                     EFFLEDRKIIKVAELPNFVVQAFVAAEDSRFFEHRGIDIQSISRAFLKNLWAGRIVQG
                     GSTITQQVAKSLYLSPEKRYIRKIKEAILAYKIDRYLTKEEILNLYLNHIYLGHGTYG
                     VEAAAQGYFGKSARHLTLPEAAMLAGLPKAPSTYSPFTHFDRARQRQSYVLERMVEDG
                     YITGADQERALNTPLTLRSVRPKEKIAPYFVEHIRRYVVEKYGSDVLYREGLEVYTTL
                     NIEAQKAAREAVERGLRELEQRQGYETGLVQGALVSMDVKTGAIKALVGGREFSKSEF
                     NRATQSRRQPGSAFKPFIYTAAFDKGLTPSSHFVDAPISFPDPSQPGGLWQPKNFDGK
                     FTGATTLRNAIVQSRNVITIKVLQDIGVDYAASYANVMGISSPLSRNLSLALGSSGVT
                     LLEMVRAYGVLANQGRRTTPYFITKIVDRTGHIFEENQPKVEQVIDPRIAFITAYIMQ
                     DVVESGTGRRVKSIGRPVAGKTGTTNDVRDAWFMGFTPSLITGVWVGFDQERSLG"
BASE COUNT          917 a         1324 c         1126 g          907 t
ORIGIN      
        1 gggaggtgaa tttttccagc tcttcctccc ggaaggtcaa ccgggccaga tgggctacat
       61 gggccacttc ttctctggtt atcttcaaga cgatacgctc cttatcccta aatccttaaa
      121 attgataagc tgtcaaaatc tttaatgttt ccgagtattt gccgcgagtc atttagccta
      181 tcaagccttc ttaaaacacc tgccagacgg gattgatctg ctccgtaacc cgtcgtagaa
      241 tctgatcctt gccctgctcg gcctcgtatt ccatgcggta gagatcgtct tccgaggccc
      301 gggtgtcccc gcgcatctcc ctcaatacca gcttgacgga ttccgtcagg ccctcgacat
      361 tataaccgcc ctccaggacg gcgaccaggc gacccccgca acaggccgcc gtaatatcca
      421 ataggatcct catcatataa acatatcctt tgggagtaac ccgcatatct cccagggggt
      481 cctggaaata gctgtcgaat cccgccgaca ggaggaccag ctccggctgg aatttcaagg
      541 ccacgggctg gaggatcccc cggaaggcct tcacatggag ggcgtcgtcg gtccgggcct
      601 tcagggggac attgacggta tatcccgatc catccccctg accggtttcg gtgaaggtcc
      661 cggagccggg ataggcagga tactgatgag tggagaaata aaggactttt ttatcccggt
      721 agaagatctc ctgggtgccg tcgccgtgat ggagatccca gtcggcgatg aggatccgct
      781 tcaggccgta tttccccagg gcataacggg cgcccacggc cacgttgttg aagaggcaga
      841 aacccgcccc cctttcctta ccggcatggt gccccggggg gcggacgaag gcgaaggcgt
      901 tgtccacctg cccgttcatc accgcctcga cggcattcag gaccccaccg acggcaagtt
      961 tggccgtgtc ataggattcg ggggaggtct gagtgtccgc atcgagggaa taataactct
     1021 tccccgccgt actggccacc aagccgatgt aggaagggct gtgaaccaga ccgatctctt
     1081 catgggtggc ataccgaggg gcgataatgg tatatttccc ttccatacca ggatgttgca
     1141 gcatggcata cagggactcc agacggcggg gggactcggg atgataatac ccttccccgt
     1201 gccgcagata ccgctcgtca accaccaccc ctgtcctgtt ggccatgaat cgactcctgg
     1261 gaaaaatccc gccttcacct ccggcgggga ccgctgtccc acccggagaa agcagcgact
     1321 tgataccaca aaacccctgt aagtgcaaaa atatttgcca ttgacaagga cttgagaggc
     1381 gctatatata ccgctattat gtttggtatc ggtctgccgg aactaatcgt catcgccgtc
     1441 gtcgccctcc tcgtcgtggg tccggagaag ctccccgggt tggccaaatc actgggaaag
     1501 ggctttcagg aattccgccg ggccacggac gatgtaacgg agtccatcaa agacacccta
     1561 aagccagacg aaatcaagaa ggatgtcgat gggttgaagg attccctcct ttacggcggg
     1621 aaggaagagc gggacaaaga cgcaaccccc aaaaaccaat cctgaagatc agccgtcaac
     1681 acaacttatt ccatgggtca aacagaagac gaaaaattac ctttaaccag ccacctggcg
     1741 gaactgcgca cccgtctcta ccgaatcctc attgccgtcg gaatgggatt cttcgcctgc
     1801 tactacttcc gggaggggct ctttcggatt atcaccaagc cccttaccga ggttttgccg
     1861 aaaaacagcc acatgatttt taccggcatg cccgaggcgt ttttcatcta tatgaagatc
     1921 gcctttttcg cctccttgct tttgaccagc ccctataccc tttaccaatt gtggaaattc
     1981 gtctcccccg gactctataa ctcggaaaaa cgttacgtgg tcccttttgt cctcatatcc
     2041 acgatcctct ttctcggcgg ggtggtcttc ggctatctcc tcgtcctgcc cacggccttc
     2101 gagttcttca tcaccttttc cacggatttc ctgaaaccca tgatctcctt ccgggagtat
     2161 ctctccttca ccatgaagtt cctgctggcc ttcgggatct ccttcgaact gccggtgttt
     2221 atcttctttc tcaccaaggt gggcgtcgtg aatgccccgc tcctgtcccg gtatcgccgc
     2281 tacgccattt tgatgatctt tatcgccgcc gccgtcctga ccccttcccc cgacgccttc
     2341 agccagatcc ttatggcggt gcccctgatg ttcctctatg aaatcagcat cctggtttcc
     2401 cgctacgcgg gaagaaagaa aaaagccggc gaggaaaacg cggccgcggg ggtcctggac
     2461 aatgagtaaa tatcgctccg acaaccgggg cttcatcagc ctcctgaccg tcgccctcgg
     2521 catagcggtg atcatggtgc tggccctgac gggatacctc ctctatttct ttttagggga
     2581 actgcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnatcat ggtgctggcc
     2701 ctgacgggat acctcctcta tttcttttta ggggaactgc cgagcgtcga taccctgaag
     2761 gactaccgtc ccagcatcgc cacccgcatc tatgccgaca acaacgaact ggtcgacgaa
     2821 ttcttcctgg aagaccggaa gatcatcaag gttgcggagc taccgaattt cgtcgtccag
     2881 gcctttgtcg ccgccgagga ctcccggttc ttcgaacaca ggggcatcga catccagagc
     2941 atctcccggg cctttctcaa gaatctctgg gcgggacgca tcgtccaggg ggggagcacg
     3001 atcacccagc aggtggccaa atctctctat ttatccccgg aaaagcgtta tatccgcaag
     3061 atcaaggagg ccatcctggc ctataagatc gaccgctacc tgaccaagga ggagatcctc
     3121 aacctctatt tgaatcacat ctacctcggt catgggacct atggcgtgga ggcggccgcc
     3181 caggggtatt tcgggaagag cgcccgtcat ctcaccctcc cggaggcggc aatgctggca
     3241 ggcctcccca aggcccccag cacctattcc cccttcaccc atttcgaccg ggcccggcag
     3301 cgccagagtt acgtcctgga acgaatggtg gaagacggct atatcaccgg agcggaccag
     3361 gagcgggcgc tgaacacccc cctgaccctc cgttccgtcc ggcccaagga aaagatcgcc
     3421 ccctatttcg tcgagcacat ccggcgctat gtcgtggaaa aatacggcag cgacgtcctc
     3481 taccgtgaag gtctggaggt ttatacaact ctgaatatcg aggcgcagaa ggcggcccgc
     3541 gaggctgtgg aaagagggct gcgggagttg gaacagcgcc aggggtatga aaccggactg
     3601 gttcagggcg ccctggtttc catggacgtg aaaaccggcg cgatcaaggc cctggtaggc
     3661 ggccgcgagt tttccaaaag cgagttcaac cgggcgaccc agtcccgccg gcagccggga
     3721 tctgccttca agcccttcat ttacaccgcc gcgttcgaca agggattgac cccctcctca
     3781 cacttcgtgg acgccccgat atcctttcct gacccctctc aaccgggcgg cctctggcag
     3841 cccaagaact tcgacggcaa attcaccggc gccacgacct tgagaaacgc catcgtccaa
     3901 tcccggaacg tgattacgat caaggtcctc caggacatcg gggtagatta tgcggcatcc
     3961 tacgccaatg tgatgggcat ctcctcaccg ctcagcagga acctgtccct ggccctgggc
     4021 agctccggcg tcaccctcct ggagatggtg cgggcctatg gcgtcctggc caatcagggc
     4081 cgccgtacga caccctactt catcaccaag atcgtggatc gcaccggtca catctttgag
     4141 gagaaccaac cgaaagtgga gcaggtcatc gatccccgca tcgccttcat caccgcctac
     4201 atcatgcagg acgtcgtgga aagcggtacg ggacggcggg ttaaaagcat cggacggccc
     4261 gtggccggaa aaacaggaac gaccaacgac gtccgggacg cctggtttat gggcttcacc
     4321 ccctcgctga tcaccggcgt ctgggtgggc ttcgaccagg aaagatccct gggg
//
LOCUS       DAOSMF010000147         4370 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_21792_length_4370_cov_22.729085, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000147 DAOSMF010000000
VERSION     DAOSMF010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4370
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21792_length_4370_cov_22.729085"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1940)
                     /locus_tag="PLT63_10035"
     CDS             complement(<1..1940)
                     /locus_tag="PLT63_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HOD99101.1"
                     /translation="MMVKIPMPETTDMTLFVRSKGEEVDRWNRRRIVDALIRETGIDV
                     ATAEAVSIDVEKGLFASGVSVLTTSLIRELVDAKLVERGLEKARRMHARLGFPLYDVD
                     QLILHRNKEDANVPHGPEGTNLVLAQGIKREYALYSIFSQEVGDAHILGDFHIHGLGF
                     VDRPFSGCQNLEYLKRFGLNLPRSLNVAKPAKHAEVLLAHMVRFGAVLQGYFAGVISW
                     NFVNLSFAPYLAGMSDREVRQFAQMLVYEFSQLTAARGGQAMFTDIHLFWEVPEPLRD
                     LPMIGPGGEPTGRTCGDYEIEAQRLARALCDVFHKGDGTGKPFIFPRPVVHITPRFFA
                     APGHEEFLCQVCAVAADKGNPCFVLEREGDVYGMGGLGIPVAPEDAATPWRQRGAAIQ
                     NISINLPRLGYRAAGDEKELFSHLEVLMDLATQAHAQKRTFLERLLSYGDQGPLSLLA
                     MNLDGTPYLRRERSAYLMGMVGLNELVRVMTGKSLHESPAAEEFGFRVIAFMAGQAKA
                     LSARYDLNLVLDQPHAETTAHRFARLDLRYFSPPAGHHVRGDITAGSLYYTNSTHLPV
                     SAPLKPAERIRREGKFHPLIGAGAAVQIWLGGKPAPPSGLADLLTWAYRETSCRQLIF
                     SPEFTACRDCGGTFPGLKSRCDR"
     gene            complement(1948..>2790)
                     /locus_tag="PLT63_10040"
     CDS             complement(1948..>2790)
                     /locus_tag="PLT63_10040"
                     /inference="COORDINATES: protein motif:HMM:NF039904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99102.1"
                     /translation="GAPRWLTFLRQLFSGIYTVDNLDYVQRDAFMTGVSLDMVDIARI
                     RYYSFFTEAGLTLHEAGISALRRFLNARLNLYANVYFHRTTRALDLHLQEIFRETMEL
                     LCPGDPREDLEAYGRLDEWRLFETVRRWTDGEDPRRRRLGREWQRLYGRQVKWKMSYS
                     TEISVENIQKGTSFALAKDYEEQIRRRLPRSLRNIALRVDLATQDPRPINPMVQTDKQ
                     INIFNPALGKTSQEPLRDIYRFIPARIVHFRVFTMNHEHDAAVAAAAEQSLENPRAAS
                     RTNI"
     assembly_gap    2791..2890
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2891..4066
                     /locus_tag="PLT63_10045"
     CDS             <2891..4066
                     /locus_tag="PLT63_10045"
                     /inference="COORDINATES: protein motif:HMM:NF024813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOD99103.1"
                     /translation="HPHRKEVLPMGTNNVIFLEVIEWFDESGQQLIHRIPETGSGEIK
                     WGAQLTVRESQAAVFFYKGKAYDAFGPGRHTLRTANIPILTKVLSIPWAMTSPLRAEV
                     YFANMKVFPNLTWGTRDPVAFRDSELGLIRLRAFGVFNIRILQPVLLINSLVGTQGVY
                     KTEEIAEYLNRVIVSRLNDFLGEKIDTIFNLPARYNELAAGLQEALVKDFSQFGLGLT
                     SLYINSITPPPEVQKAIDDKSKLGVFDDLNKLLKMKAAMALENASLNQGETGSALGMG
                     MGFMMPAMFADAWRSSPGAAGSGGGVPGTGGETAAQCPDCRQSVPREARFCPYCGHQL
                     LIIRQCGACGKNLPASAKFCPQCGSPAERKPEPKKCPQCGTENLSNSLFCNRCGERI"
     gene            4053..>4370
                     /locus_tag="PLT63_10050"
     CDS             4053..>4370
                     /locus_tag="PLT63_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99104.1"
                     /translation="MNGSNWQIQHQCPQCGAPVILEETDRILSCAFCRTRLLIVSPGP
                     FHYYIPAPAAAESVYIPYWRLRGLAYQLKPLDITDTYIDTNRRALPLPGLPASLGVRP
                     QAMK"
BASE COUNT          822 a         1347 c         1329 g          772 t
ORIGIN      
        1 cggtcgcacc gcgacttgag gcccggaaag gtgccgccgc aatcccggca ggcggtaaac
       61 tccggggaaa agatgagctg tcggcaggag gtctctcgat aggcccaggt gagcagatcc
      121 gccaggccgg acggcggcgc cggctttccc cccagccaga tctggacggc ggcgccggcc
      181 ccgatcagag ggtggaattt cccttcccgg cggatacgtt ccgcgggctt caggggggcg
      241 gaaacgggca gatgggtgga attcgtgtaa tagagcgacc ccgccgtgat atcgccccgc
      301 acatggtgtc ccgcaggcgg ggaaaaatag cggaggtcca ggcgggcaaa gcgatgcgcc
      361 gtcgtttcgg catggggctg gtccaggacc aggttaaggt cgtaacgagc gctcaaggcc
      421 ttggcctgcc cggccataaa ggcgatcacc cggaacccga attcctccgc cgccggtgat
      481 tcatggaggg attttcccgt catgacccgc accagttcgt tcaggcccac cattcccatg
      541 agatatgcgg accgctcccg ccgcagatag ggggtgccgt cgagattcat ggccagcaag
      601 gacagagggc cctgatcgcc gtacgaaagc aatctttcca gaaacgtccg cttctgggca
      661 tgggcctggg tggcgagatc catgaggacc tccagatgag agaagagttc cttctcgtct
      721 ccggcggccc ggtagcccag gcgcgggagg ttgatgctga tgttctgaat ggccgccccg
      781 cgctgccgcc agggggtggc ggcatcttca ggcgccaccg gtatccctag cccccccatc
      841 ccatatacat ccccctctct ttccaggacg aagcagggat ttcccttgtc tgcggcaacc
      901 gcgcagacct gacagagaaa ctcctcatgc ccgggggcgg caaaaaagcg gggggtgatg
      961 tgcaccaccg gacggggaaa gataaagggc ttgcccgtgc cgtctccttt gtggaataca
     1021 tcgcacagtg ctcgggccag acgctgcgcc tcgatttcgt aatccccgca ggtcctcccc
     1081 gtgggctctc ctcccggacc gatcatgggc aaatcccgca gtggttcggg gacctcccag
     1141 aagagatgga tgtcggtgaa catggcctgc ccaccccggg ccgccgtcag ttgggagaac
     1201 tcgtaaacga gcatctgggc gaactgccgt acctcccggt cggacatgcc ggccaggtaa
     1261 ggggcgaagg ataggttgac gaagttccaa ctgatgacgc cggcgaaata gccctgcagg
     1321 acggctccga atcgtaccat gtgggccagc agcacctcgg cgtgcttggc gggcttggcg
     1381 acattcaggg agcggggcag attcaggccg aatctcttca gatattccag attctggcag
     1441 cccgagaagg gacggtccac aaatcccagg ccgtggatgt gaaaatctcc caggatgtgg
     1501 gcatcgccta cctcctggga gaagatgctg tacagcgcgt attcccgttt gatcccctgg
     1561 gccaggacca gattcgtccc ttccggcccg tgcgggacgt tggcgtcttc cttgttgcga
     1621 tggaggatga gctgatcgac gtcatagagg ggaaagccga ggcgggcgtg catccgccgg
     1681 gccttctcca gcccccgttc cactagtttc gcatccacga gttcccggat cagggacgtc
     1741 gtcagtaccg agaccccgga ggcaaagagg cctttctcca cgtcgatgct caccgcctcc
     1801 gccgtcgcga cgtcgatccc cgtttcccgg atcaaggcgt caacgatgcg ccggcggttc
     1861 cagcggtcca cctcctcgcc cttggaccgg acgaagaggg tcatatcggt cgtttccggc
     1921 atcggtatct tcaccatcat gatccgctca tatattcgtc cgcgatgccg cgcggggatt
     1981 ctccagggac tgctccgccg cggcggcgac cgcggcgtca tgctcatgat tcatggtaaa
     2041 gacccggaag tgaacgatcc gggcgggaat gaaccggtag atgtcccgca ggggctcctg
     2101 ggaggtcttg cccagggccg ggttgaagat gttgatctgc ttgtccgtct ggaccatggg
     2161 attgatgggc cgggggtcct gggtggccag atccacccgg agggcgatat tccgcaggga
     2221 tctcggcaga cggcggcgta tctgctcctc ataatctttc gccaaagcga agctcgttcc
     2281 cttctggatg ttttccaccg atatctccgt ggaataggac atcttccatt tcacctgccg
     2341 gccgtacagg cgctgccact cccgaccgag acgcctgcgg cgggggtctt ccccgtcggt
     2401 ccagcggcgg acggtctcga agagccgcca ttcatcgaga cggccatagg cctccagatc
     2461 ctcccgggga tcgccggggc agaggagttc catggtctcc cggaagatct cctggaggtg
     2521 gaggtccagg gcccgggtgg tgcgatggaa atagacgttg gcatagaggt tgaggcgggc
     2581 gttcaggaaa cggcgcaggg cggagatccc cgcttcatgg agggtcaggc cggcctcggt
     2641 gaagaaggag taatatctga tccgggcaat atccaccata tccagggata cgccggtcat
     2701 gaaggcgtcg cgctgcacgt aatcgagatt gtccaccgtg tagatcccgg aaaaaagctg
     2761 gcggaggaag gtcagccacc ggggcgcccc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn catccccaca ggaaagaggt gctccccatg ggaacgaaca atgtcatttt
     2941 tctcgaagtc atcgaatggt tcgacgaatc gggccaacag ttgatccaca gaattcccga
     3001 aacgggctcc ggcgaaataa aatggggggc ccagctcacg gtccgggaga gccaggccgc
     3061 cgttttcttt tacaaaggca aggcctacga cgccttcggg ccgggacgcc acaccctgag
     3121 aacggccaac atccccattc tcaccaaggt cctcagcatc ccctgggcga tgaccagccc
     3181 cctgcgggcg gaggtctatt tcgccaatat gaaggtcttt cccaatctca cctgggggac
     3241 cagggacccg gtggccttcc gggacagcga actggggctg atccgcctcc gggccttcgg
     3301 ggtgttcaac atccgcatcc tccagcccgt cctgttgata aacagcctcg tgggcaccca
     3361 gggggtctat aagacggagg agatcgccga atatctgaat cgcgtcatcg tctcccgtct
     3421 gaatgatttc ctgggggaaa agatcgacac gatcttcaac ctccccgccc ggtacaacga
     3481 actggccgcg ggacttcaag aggcgctggt aaaggacttc agccagttcg gcctggggct
     3541 gacctcgctg tacatcaact ccataacccc gccgccggag gtgcagaagg ccatcgacga
     3601 caagagcaag ctgggggtct tcgacgacct caacaagctg ctgaagatga aggccgccat
     3661 ggccctggaa aacgcctccc tgaaccaggg ggaaacggga tccgccctgg ggatggggat
     3721 gggcttcatg atgcccgcca tgttcgccga tgcctggcgt tcttccccgg gcgcggcggg
     3781 aagcgggggc ggcgtacccg ggaccggcgg cgagaccgcc gcccaatgcc cggactgccg
     3841 ccagtccgta ccccgggagg caagattctg cccctattgc ggccaccagc tcctcatcat
     3901 ccggcagtgc ggggcctgcg ggaagaatct gccggccagc gccaagttct gccctcaatg
     3961 cggcagtccg gcggaacgga agcccgagcc gaaaaaatgc ccccagtgcg gtacggaaaa
     4021 tctttctaac tcattgttct gcaaccgttg cggtgaacgg atctaactgg cagattcagc
     4081 accagtgccc gcagtgcggc gcaccggtca tcctggagga aacggaccgc atcctgtcct
     4141 gcgccttctg ccggacccgt ctgctcatcg tctcacccgg cccctttcac tattacatcc
     4201 ccgcgccggc ggcggcggag tccgtctata ttccctactg gcgtctccga ggccttgcct
     4261 accagctcaa gcccctggat attaccgaca cctatatcga caccaatcgc cgggccttgc
     4321 ccctgccggg gctccccgcc tccctggggg tccgtcccca ggccatgaag
//
LOCUS       DAOSMF010000148         4339 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_22022_length_4339_cov_19.552288, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000148 DAOSMF010000000
VERSION     DAOSMF010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4339
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22022_length_4339_cov_19.552288"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="PLT63_10055"
     CDS             complement(<1..517)
                     /locus_tag="PLT63_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOD99105.1"
                     /translation="MTELSRHVNRQLGLLITRRGEIAYVIVGDHQGIVIPNLDGFRAS
                     SMRFKGLRLVHTHLHGEKLTDDDFTDLALLRLDMISAVEALPDGLPGIVHSAHLIPEN
                     NEGKFWACLESRRPAEIGIDFSAFIAALEGEFVRRQKTRKVEAADRGILIRVETDPMA
                     DAPLSLAELKEL"
     gene            complement(805..1008)
                     /locus_tag="PLT63_10060"
     CDS             complement(805..1008)
                     /locus_tag="PLT63_10060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOD99106.1"
                     /translation="MPLYDFECRECGHKFEAFRKMREAADDLTCPECGAGNPQKLVTA
                     FQTNAWSSFLDAMEKRVNPQKFK"
     gene            complement(1324..>2873)
                     /locus_tag="PLT63_10065"
     CDS             complement(1324..>2873)
                     /locus_tag="PLT63_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99107.1"
                     /translation="LIMAFAMPAAAVDVKFSGQYYALGAYVDNHSLLDKDEAAGFGAA
                     TTWGTVDASQANYATRDARTLRGPYAWYAQRFRTSIEFQVVEGLKLVTRFDALEKHWG
                     DQAWAGGTGETQSRTQTPLGSPDPNGVRRGANVPKAQENIEWERAYLDFNVPFGKFQV
                     GYMQFLMWGTDFLNTPLTAGGIRYFYSRGPWMAVAAIEKRGEMVGLNAGNIGVANDAD
                     KDVYDLGVIYKLKRGEVGLLYQYNRNSISKWEQGNGFTTSIMGVYPYTKLTFGNFFIE
                     AEGVYGWGDLRSYESTTRAATVGAPAAGRDLQNITLSALGAFVHAKYNWKPAYFGAQF
                     VYLSGDDNDSPDKVTGSIAGALQGGYGFDRALILWNSAYGDNLGNLRGNVAGVTANRR
                     TGASYQINQFMDNVWFYQIYAGIKPTPKLDFKAALSIASADKKPKSDVGAVGSIGYYG
                     TRVQEFDSNKYGTELDITGSYKIYDNLTYMVGFGYLWVGDYFKGYDVNAKTRDNYLLT
                     HRLTLNF"
     assembly_gap    2874..2973
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3220..>4339
                     /locus_tag="PLT63_10070"
     CDS             3220..>4339
                     /locus_tag="PLT63_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOD99108.1"
                     /translation="MAEEKVQLKEIYPVPEKVKKMAFISGREAYDKLWKRSIEDPNGF
                     WSEIAKEYVEWFKPFDKVMDYNFDIKKGDIYVKFFEGGKLNVSYNCLDKNLKTRANKV
                     AIQWEGNEPGEDRAITYQQMYEEVCKFANVLKGLGVKKGDRVCLYLPMIPELAISMLA
                     CTRIGAIHSIVFGGFSSDSLRDRIQDSSAKVLVTCDGTFRGAKAVPQKDSADAAVAEC
                     PSVEKMIVVKRVGDKIKCNFKAGRDLWWNDVMAKASADCPPEWMDAEDPLFILYTSGS
                     TGKPKGVVHTTGGYLTFVAYTHKMIFDYHEEDIYWCTADIGWVTGHSYIVYAPLCNGA
                     TSVMFEGVPNYPDMSRFWKIVEKYKVNIFYTAPTAIRAI"
BASE COUNT          872 a         1191 c         1272 g          904 t
ORIGIN      
        1 ccagttcctt cagctccgcc agggagaggg gggcgtcggc catgggatcg gtttcaaccc
       61 ggatcaggat cccccgatcg gcggcctcga ccttccgggt cttctggcgc cggacgaatt
      121 ccccttccag ggcggcgatg aatgcggaga agtcgatccc gatctcggcg ggacgacggg
      181 attccaggca ggcccagaac ttgccctcgt tgttttccgg gatgagatgg gcgctgtgga
      241 cgatgccggg gagaccgtcg gggagggcct ccacggcgga gatcatgtcg aggcgcagga
      301 gggccaggtc cgtgaaatcg tcgtcggtaa gcttctcgcc gtggagatgg gtatggacca
      361 ggcgcaatcc cttgaagcgc atcgaggagg cgcggaagcc gtcgagattg gggatgacga
      421 tcccctggtg atcgcccacg atgacatagg cgatctcgcc gcggcgggtg atcagtaatc
      481 cgagctgacg gttgacatgc cgcgacagtt ccgtcaactg ccgggccatc tcctgggtca
      541 ggaccgtatc cggggggagg cgacgccggt aaaagagccc gatgcgccta agctgctccg
      601 ccccgagccc attcaggttt ccatagatcc tctggatggt gatgcctcct cgaaattgct
      661 gattctttgc ccacctgacc cgcgccgctg cagcagccga gtctctaatc cgtttccgga
      721 cggaaagagg cgggaatcag tcgctccgtg ccccctgatc ttcacgccgc cgtgaatgat
      781 gccggcctgc tggcagggtt cgttctattt gaatttctga gggttgaccc gcttttccat
      841 ggcgtcgagg aaactggacc aggcgttggt ctggaaggcg gtgacgagtt tctgggggtt
      901 accggcgccg cattccgggc aggtcaaatc gtccgctgcc tcccgcatct tgcggaaggc
      961 ctcgaatttg tgtccgcatt cgcggcattc aaaatcgtac aggggcatta tataacctct
     1021 tgaaaatata tattattacg atacgagaag gttttgagga gttttaccag cacggcgata
     1081 acggcggcct gtgcaacaaa cagaccggca acccctttga tgatttccgc cttcatattt
     1141 gctctggggg taatatacgc gagaattaag gggttggcaa atgttttttg agagaggcca
     1201 aagacccttg ctttgtttgg atgcaaattg tgtctagcgg gtgttgtcta tgcgcagggt
     1261 aaaaaaggcc ccggagatta gctctccggg gccggcactg ccagttgctg tcggtgagac
     1321 gacttagaag ttcaaggtta gccgatgggt aaggaggtag ttgtccctcg tcttggcatt
     1381 gacatcgtag cccttgaagt agtcgccgac ccacaggtag ccgaagccca ccatgtaggt
     1441 aaggttgtca tagatcttgt aggaaccggt gatgtcgagt tccgtaccgt acttgttgga
     1501 atcgaattcc tgaaccctcg ttccatagta accgatggat cccacggcac ccacgtcgct
     1561 cttgggtttc ttgtccgcgc tggcgatcga caaggcggcc ttgaagtcca acttcggggt
     1621 gggtttgatc ccggcataga tctgatagaa ccagacgttg tccatgaact ggttgatctg
     1681 gtagctggcg ccggttctgc ggttcgcggt tacaccggcc acatttcctc ggaggttccc
     1741 gaggttgtcg ccgtaggcgg agttccagag gatcagggcg cggtcgaagc cgtagccgcc
     1801 ctggagggcc ccagcgatgg agccggtcac cttgtccggg ctgtcgttgt cgtcgccgct
     1861 cagatagacg aactgggcgc cgaagtaggc gggcttccag ttgtacttgg catggacgaa
     1921 ggcgcccaga gcggaaaggg tgatgttctg gaggtctcta cccgctgccg gggcgccgac
     1981 tgttgctgcc ctggtggtgc tctcatacga acggaggtcg ccccagccgt aaacaccctc
     2041 ggcctcgatg aagaagttgc cgaaggtcag cttggtgtag ggatacacac ccatgatgga
     2101 ggtcgtgaag ccgttgccct gttcccactt gctaatggag ttgcggttgt actgatagag
     2161 gaggcccact tcgcctctct tcagcttgta gatgacgccc aggtcataga cgtccttgtc
     2221 ggcgtcgttg gccacgccga tgttaccggc gttcaaaccg accatttcgc ccctcttttc
     2281 tatggccgcc accgccatcc agggaccccg gctgtagaag tagcgaatgc cgccggcggt
     2341 aaggggagtg ttgaggaagt cggtacccca catgaggaac tgcatgtaac cgacctggaa
     2401 cttaccgaag ggaacgttga aatccagata ggcccgttcc cactcgatat tttcctgcgc
     2461 cttgggaacg ttggcgccgc gtctcacgcc attgggatcg ggagatccca ggggagtctg
     2521 ggtccgggac tgggtctcac cggtgccccc ggcccaggcc tggtcgcccc agtgcttttc
     2581 cagggcgtca aagcgggtga cgagtttcag gccttccacg acctggaact cgatgctggt
     2641 ccggaaccgt tgggcatacc aggcgtaggg accacgtaag gtccgggcat cacgggtggc
     2701 gtaattggcc tgcgaggcat ccacggtgcc ccaggtggta gctgcgccaa agcccgctgc
     2761 ctcatccttg tccagcagcg agtggttgtc cacgtacgcg cctaaggcgt agtactggcc
     2821 gctgaacttt acatctaccg ccgcggcggg catggcgaag gccatgatca gccnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngccaaaa tatctcttga catgaggtac
     3001 atattgatat actgcaagtc gcgtcaagtc gggacctcat ggtcatctcg acttatgcct
     3061 tgtcaacgcc gttttattta gggtttccga cccgccggtc gtgttttcgc aagcgtttaa
     3121 ccgttaattg ccggcggata tatcccgtcc gggaccgggg cgcccgaagc agtatcctga
     3181 caagctcatc caataaattt agggaggtaa caaaacagta tggcagaaga gaaggttcag
     3241 ttgaaagaga tttatcccgt accggagaag gtcaagaaga tggccttcat cagtggccgg
     3301 gaagcctacg acaagctctg gaagcgttcc attgaagatc cgaatggttt ctggtcagag
     3361 attgccaaag agtatgtgga atggttcaag cccttcgaca aggtcatgga ctacaacttc
     3421 gatatcaaga agggcgatat ctacgtgaag ttcttcgaag gcgggaagct gaacgtttcc
     3481 tataactgcc tcgacaagaa tctcaagacc cgggccaaca aggtcgcgat ccagtgggaa
     3541 ggcaacgaac ccggcgaaga ccgggccatc acctatcagc agatgtacga ggaagtctgc
     3601 aaattcgcca atgttctcaa agggttggga gtaaaaaaag gcgaccgtgt ctgcctgtat
     3661 ctccctatga tccccgagct ggccatcagc atgctggcct gcacccgcat cggcgccatc
     3721 catagcatcg tcttcggcgg cttcagctcc gactccctga gagacagaat tcaagattca
     3781 tccgccaagg tgctggtcac ctgtgacggg accttccggg gcgccaaggc cgttccccag
     3841 aaggacagcg ccgacgccgc cgtcgccgag tgtccctcgg tggaaaagat gatcgtcgtg
     3901 aagcgcgtgg gcgacaagat caagtgcaac ttcaaggccg ggcgcgacct ctggtggaac
     3961 gatgtgatgg ccaaggcctc cgccgattgc ccccccgaat ggatggacgc ggaagatccc
     4021 ctctttatcc tctatacctc cggttccacg ggcaagccca agggcgttgt ccacaccacc
     4081 ggcggctatc tcaccttcgt cgcctacacc cacaagatga tcttcgatta ccatgaagag
     4141 gacatctact ggtgcaccgc cgacatcggc tgggtaacgg gacattccta catcgtctac
     4201 gcgccgctgt gcaacggcgc cacttccgta atgttcgaag gcgtgcccaa ctaccccgac
     4261 atgagccgct tctggaagat agtggagaag tacaaggtca acatcttcta cacggccccc
     4321 acggccatcc gcgccatcg
//
LOCUS       DAOSMF010000149         4275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_22423_length_4275_cov_25.635782, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000149 DAOSMF010000000
VERSION     DAOSMF010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4275
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22423_length_4275_cov_25.635782"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="PLT63_10075"
     CDS             <1..348
                     /locus_tag="PLT63_10075"
                     /inference="COORDINATES: protein motif:HMM:NF014661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HOD99109.1"
                     /translation="MEVATTRGLLFQQHGAGRLIEARPLYLFEAFAGDVLGDDPPTKS
                     LSELAIGYSDDILADILSVSARLREHGLSQVIVINLTRDEIQIPTARVIIPGMEVYGF
                     DPTRVGERLYHFL"
     gene            361..732
                     /locus_tag="PLT63_10080"
     CDS             361..732
                     /locus_tag="PLT63_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99110.1"
                     /translation="MLSEAEILERARYCYLVFFQLSHLQELNLADPFQYLHILKKSSL
                     QLAEDEFIIATMQEEICQGNPDGGLSYLIALYEGFIHAYCETVEKQLPDIRDEIPGDF
                     LGQLAAEMVPDEDQGQEQKQP"
     gene            1002..2054
                     /locus_tag="PLT63_10085"
     CDS             1002..2054
                     /locus_tag="PLT63_10085"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HOD99111.1"
                     /translation="MARIRGKSKTAEILTLSEAVKKYAQSGIQVAFSGFTGFNRNPMA
                     FAWEMVRQGVKDIHCIDRHGSCCTWLLNAVNAISVYETDWMGWGEMAGKLDVNLNRNY
                     KEGKIILEDYSHGAMAMRFLAGAIGAPFIPYYAPLGSDLYSEKYDALGKAGKRDGKVA
                     NIPLKKFIQMTEPFWGDGECVLLPAARPDLAVIHVSQAGDKGTATWRGVGTIDKELSF
                     ACEKVVIIAEEIVPEAEMRKYPEANQIPYFVVDAIVECPWGAFPSSVPYYYDYDVPFM
                     RGMDAASRNTDDLKKWLDEWIFGPASWEDFLIKLGAQKLLSLKAESTGYSTRMLRGKK
                     PAPRMKMPLSVARSGY"
     gene            2076..2978
                     /locus_tag="PLT63_10090"
     CDS             2076..2978
                     /locus_tag="PLT63_10090"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HOD99112.1"
                     /translation="MAKSMEQLMDLIDQIPNTPANVGEFILPELMAIAIAHEVRNDDI
                     VFAGTGLPMVGIMTANFTTAPNALLIYESGICDGKTMHVPMSVCDQRAANMSSTLGGL
                     VDTFGYYLQQGYVSLGFLGGAAIDKYGGVNVTSIGDYFNPSHRFTGSGGNSDIGTLSK
                     RVAYIILQEKRRFVERNDYTTTPGWWCWDFKTNEWKPKKEVWKGTPYADSGPCGVVTN
                     MGVYRFDDKGEIYLETHHPGVTVEQVKENCGFDLNVSLVKGETPRPTYQELFVLREFV
                     DPELIFLPAKTPYTPDIQAIIDKG"
     gene            3113..4270
                     /locus_tag="PLT63_10095"
     CDS             3113..4270
                     /locus_tag="PLT63_10095"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOD99113.1"
                     /translation="MNLDLTEDQKMIQDTARNFAKSELEPVAAKLDEEGDRETFLKNL
                     RKLAELGLMGIAVKSEYGGSEAGAVAFSLAITEIARACASTAVTCSVSNMVCEVIQAI
                     GSEEQKRAYIPKICSGEYYAGGFGLTETTAGSDPAGMRCSAVLDGNEWVLNGSKIFIT
                     SAEYAGVFVVWAVTDKAAPKGKGISCFLVENGLPGFTVGRAEHKMGQHASATNELLFE
                     DCRIPKTAMMGKPNDGFRTAVGELAGGRIGIGSLGLGCGLAAIEYAAKYAVERKQFDQ
                     SISNFQAIQWMIAESYTELEAARLLLMNAAYQKECGRFFAREASMAKYYATEAGERAC
                     YNAIQILGGYGYTKEFPVERHARDVRITSIYEGTNQIQRVIIARDILSKLK"
BASE COUNT         1037 a         1167 c         1167 g          904 t
ORIGIN      
        1 atggaagtgg ccaccacccg gggactcctc tttcagcagc acggcgccgg ccggctcatc
       61 gaagccagac cgttgtattt gttcgaggcc ttcgccggag atgtcctggg ggacgatccg
      121 ccgactaaaa gcctctctga actagccatc ggctacagcg acgacatcct cgcggacatc
      181 ctttccgtct ctgcgcggct ccgggaacac ggcctgtccc aggtaatcgt gattaacctg
      241 acgcgagatg aaatccagat tcctaccgcc cgggtcatca tccccgggat ggaggtttat
      301 ggattcgacc ccacccgcgt tggggaacga ctctatcatt ttttgtaagg acgggaataa
      361 atgctctcgg aagcggaaat tctcgagcgg gccagatact gctatcttgt ttttttccag
      421 ttgagtcacc tgcaggagct gaatctggcc gatccatttc aatatctcca tatcctcaag
      481 aaatcttctc tacagcttgc ggaggatgaa ttcatcatcg cgaccatgca ggaggaaatc
      541 tgccagggaa accccgacgg cggcctatct tatctcatcg ccctttatga aggcttcatc
      601 cacgcctact gcgaaacggt ggaaaagcaa ctcccggata tccgcgacga aatccccggc
      661 gattttctcg ggcaactggc ggcggaaatg gtccctgatg aagaccaggg gcaggaacaa
      721 aaacaacctt aataaccttt acgggaaaaa tgcaaaattt atgtattttc cccttgactc
      781 tttaagcggg attcaatata aaataactgc ttcttaggtt cactggcaag ttcgcaaatc
      841 ttccaaaagt atttgattat atatatttta agtatcaccg taagttaata gatcgtccaa
      901 aaggttttgg attctttgat gaaaggaggt gaaacgaaag ggggattttt tatttttttc
      961 tttatttgta gggggttaat caacaacaaa aggaggtagt tatggcaaga attcgtggga
     1021 agtcaaaaac cgctgaaatc ttgacccttt ccgaagcggt caagaaatac gcgcaaagcg
     1081 gaattcaagt agcattcagt ggttttaccg gtttcaacag aaatccgatg gccttcgcct
     1141 gggaaatggt ccggcagggg gtcaaagata ttcattgtat cgacagacac ggcagttgct
     1201 gcacctggct tcttaatgcc gttaacgcca ttagcgtcta tgaaacggac tggatgggct
     1261 ggggcgaaat ggcgggtaag ctcgacgtca atcttaaccg gaactataaa gaaggcaaga
     1321 tcatcctcga ggattactct catggcgcta tggccatgcg gttcctcgcc ggcgccatcg
     1381 gcgccccctt cattccctac tatgcgcccc tgggatcaga cctttacagc gagaaatacg
     1441 atgccttggg gaaggccggc aagcgagatg gcaaggtggc caatatcccc ttgaagaaat
     1501 ttatccagat gaccgaaccc ttctggggtg acggcgagtg cgtccttctg cccgccgccc
     1561 gtccggatct ggcggtcatc catgtatccc aggccggtga caagggcacg gcgacttggc
     1621 gcggtgtcgg caccatcgac aaggaactct cctttgcctg tgaaaaggtc gttatcatcg
     1681 ccgaggaaat cgtccccgaa gcagagatga gaaaatatcc cgaagcaaac cagatcccct
     1741 actttgttgt tgacgctatc gtggaatgcc cctggggtgc cttcccgagc tcggtaccat
     1801 actactacga ttatgatgta ccctttatga gaggtatgga cgccgcttcc cgaaataccg
     1861 acgatctgaa gaaatggctg gatgaatgga tattcgggcc ggcctcctgg gaagattttc
     1921 taatcaagtt gggggcacaa aagcttctct ctttgaaggc ggaaagcacc ggatacagca
     1981 cccgcatgct gagaggcaag aaacccgcac cccggatgaa gatgcccttg tccgtggccc
     2041 gcagcggtta ttaagagaaa ggggggagaa ttaatatggc aaaatcaatg gaacaattga
     2101 tggatcttat cgatcagatt cccaatacgc cggcaaacgt aggggaattc atcctgccgg
     2161 aacttatggc catcgccatc gcccacgagg tccgcaacga cgacatcgtc ttcgccggca
     2221 ccggtctgcc catggtgggc atcatgacgg ccaacttcac caccgctccc aatgcgctgc
     2281 tgatctatga atccggcatc tgcgacggca agaccatgca cgtccccatg tccgtctgcg
     2341 accagcgggc ggccaacatg agctcgacgc tgggcggtct cgtggatacc ttcgggtatt
     2401 acctccagca gggttacgtg tctctgggct tcctcggcgg cgcggccatc gacaagtacg
     2461 gcggcgtcaa cgtcacctcc atcggtgatt acttcaatcc ttcccaccgg ttcaccggat
     2521 cgggcggcaa ctccgacatc ggaacattgt ccaagcgcgt ggcctacatc atcctccagg
     2581 aaaaacgccg cttcgtcgag aggaacgact acaccaccac cccgggctgg tggtgctggg
     2641 atttcaagac caacgagtgg aaaccgaaaa aagaggtatg gaaaggtacg ccttacgcgg
     2701 attccggccc ctgcggcgtc gtgaccaaca tgggcgtcta tcgcttcgac gacaagggtg
     2761 agatctattt ggaaactcac catcccggcg tgacggtgga gcaggttaag gaaaattgcg
     2821 gctttgacct caacgtttcc ctggtaaaag gcgaaacacc tcgtccgacc tatcaggagc
     2881 tcttcgtcct ccgcgaattc gtggatccgg agttgatctt cctgccggca aaaactcctt
     2941 acacgcctga cattcaggcc attatcgaca aaggctaacg acacatgacg aaaaagaggg
     3001 gaagcttgct tcccctcttt tttaccttca ccgacaataa ttgggaaatt acccgcctgt
     3061 tttccgaaca agcggcgtca ataatcatca tacaggaagg ggaaaagacg caatgaattt
     3121 agatttaacg gaagaccaaa agatgattca ggatacggcc cggaattttg ccaagtcgga
     3181 attggaacca gtggccgcaa aactagatga ggagggagac cgtgaaacct tcctgaagaa
     3241 cctgagaaaa ctggccgagc tgggtctgat gggtatcgcc gtcaaatcgg aatacggtgg
     3301 ttcggaggcg ggggcggtgg ccttcagtct cgccatcacg gaaatcgccc gggcctgcgc
     3361 ctctacggcc gttacctgtt ccgtgagcaa tatggtctgc gaggtcatcc aggccatcgg
     3421 ctcggaagag cagaaaaggg cctacattcc gaagatctgc tccggagagt actatgccgg
     3481 cgggttcggc ctcacggaga cgacagcggg ttcggaccct gcggggatgc gctgctccgc
     3541 cgtcttggac ggcaacgaat gggtcttgaa cggcagcaag atcttcatca ccagcgccga
     3601 atacgccggg gtgtttgtcg tctgggccgt tacggacaag gccgccccca agggcaaggg
     3661 catcagttgc ttcctcgtag aaaacggcct tccgggcttc accgtaggcc gggcggagca
     3721 taagatggga cagcatgcgt cggcaaccaa cgagttgctc ttcgaagact gccgaattcc
     3781 caaaaccgcc atgatgggca agccgaacga cggcttccgc accgccgtgg gcgagctggc
     3841 aggcggacgg atcggcattg gctccctggg cctgggctgc ggcttggcgg ccatcgaata
     3901 cgccgccaag tacgccgtcg agcggaaaca gttcgatcag tccatcagta acttccaggc
     3961 catccagtgg atgatcgccg agtcctatac ggaactggag gcggcccgcc tgctcctcat
     4021 gaatgcggcc taccagaaag agtgcgggcg cttcttcgcc cgtgaggcct cgatggccaa
     4081 gtattacgcc acggaagcgg gagaacgggc ctgctacaac gccatccaga tcctcggcgg
     4141 ttacgggtac acgaaggaat tcccagtgga acgccacgcc cgggacgtcc ggatcacctc
     4201 gatttatgaa gggacgaacc agatccagcg cgtcatcatc gctcgggaca tcctgagtaa
     4261 attgaagtaa ccaac
//
LOCUS       DAOSMF010000150         4236 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_22689_length_4236_cov_26.595790, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000150 DAOSMF010000000
VERSION     DAOSMF010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4236
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22689_length_4236_cov_26.595790"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..1346)
                     /locus_tag="PLT63_10100"
     CDS             complement(51..1346)
                     /locus_tag="PLT63_10100"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOD99114.1"
                     /translation="MYWEKDIETISRDNLAVLQLENLRHTLAWAAKSFYYGELFKKIG
                     LAPENVRSRADLTKIPPTTKDDLRAHWPYGFVAVPRDELVRMHSSSGTTGRATVIFHT
                     AGDLSEWTNLLARCMYMSGMRKSDVFQNMMTYGLFTGGLGFHYGAERIGALVIPAGAG
                     NSKRQIQLLKDFETTVIHIIPSYALHLSTVFEELRMDPKDTKIRIAYVGAEPHSEKMR
                     QKIEALYGFKAYNSYGLSEMNGPGVAFECPVQNGLHIWEDNFIAEILDPATLQPVPDG
                     EEGELVLTTLRREGMPILRYRTKDLTRIIPGDCPCGRTHRRIERIKGRTDDMMILKGV
                     NIFPIQIEKKLMEIPGVGKNFLIILDREGFNDHMTVKVEVERQFFDGDLKHLEQLRKR
                     IVEELKSDILITPKVDLVTPESLPQSEGKAKRVIDNRKD"
     gene            1576..>2120
                     /locus_tag="PLT63_10105"
     CDS             1576..>2120
                     /locus_tag="PLT63_10105"
                     /inference="COORDINATES: protein motif:HMM:NF025704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HOD99115.1"
                     /translation="MSKEMRGMVMMLLVLSVSFMFFVGCAKPPTQEMGSAEKALTEAK
                     AKEADLYAADLFKKAEDALKKAKDYVAQKSYKEAKAAAEEAAQTAGQALAAIEGNKAK
                     MKQEATQMIEEAEKSLAEIKALTAAAIKKKLPINREELQGMIGKAEVDLVGVKNTLQE
                     QKIRPAYDQVKAILAQLKGKIG"
     assembly_gap    2121..2220
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2221..2687
                     /locus_tag="PLT63_10110"
     CDS             <2221..2687
                     /locus_tag="PLT63_10110"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HOD99116.1"
                     /translation="EIILVTNEPQLYLDQDVAIVTDLEKGKGPLMGIYTGLFWAASET
                     AFVAACDMPFLSAPFIRYLRDQSGGDVDIVVPETADGLQPLHAVYSRRCLPAIKARLD
                     RDRLKVTEFYRGMKVRTVSAETIRRFDPHGRMFLNVNTVEEFANLRQSEQPS"
     gene            3001..>4236
                     /locus_tag="PLT63_10115"
     CDS             3001..>4236
                     /locus_tag="PLT63_10115"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HOD99117.1"
                     /translation="MAEQKTFNRWLVVVGAMLIQVSLGAVYIYSVFKPALSAKFPTWS
                     PTDLALPSQLILAFFAIGVIFAGKIQDKIGPRTVATVGGAMLGVGLIIASFASDLMMF
                     TIAFSVIGGLGIGAAYVCPIATCVKWFPDKRGMITGLAVAGFGAGALVFTPIAKAFIA
                     SSGIMSTFMYLGIIFLVAVVAGAQLMVVPPAGYKPEGWNPPAPAANAGGAKAAGGDFT
                     TMEMLKTPQFYFLWITYFAGCMAGLMIIMNITNMWQSPSMIELAKTMPTVTKDAFSTV
                     ANQGATAVMVVAILNAAGRLVWGQVSDVIGRKMTLIIIFAYAGVIMLLLNTFTSYMMF
                     LVGVCSVGFCFGGFLGLYPAVTADYFGTKNVGANYGLMFAAYGVGGLIGPWLAPKLMT
                     IVGQIPYEAMDKGALIIKQF"
BASE COUNT          774 a         1263 c         1207 g          892 t
ORIGIN      
        1 ctctggagct ttttacttcg tcgtctccat catgactttt tacgaattca tcaatcttta
       61 cggttgtcaa tcaccctttt ggccttcccc tcggactggg gcaggctctc cggcgtcacc
      121 agatccacct tgggggtgat gaggatgtcg cttttgagct cttcaacgat gcgtttccgc
      181 aactgttcga gatgcttcaa atcgccgtcg aaaaactgcc tttccacttc cacctttacg
      241 gtcatgtggt cgttgaatcc ctcccgatcc aggatgatga ggaaattctt gcccacgccg
      301 gggatttcca tgagcttctt ttcgatctgg atggggaaga tgttgacccc cttgaggatc
      361 atcatgtcgt ccgtacgccc cttgatccgt tcgatccgcc ggtgggtccg cccgcagggg
      421 caatccccgg ggatgatgcg ggtgaggtcc ttggtccggt agcggaggat cggcatgccc
      481 tcccggcgga gggtggtcag caccaactcc ccctcctcgc cgtccgggac gggctggaga
      541 gtcgccggat caaggatctc cgcgataaag ttgtcttccc agatgtggag gccgttctga
      601 acgggacact cgaaggccac gccggggccg ttcatctccg agaggccgta ggaattgtag
      661 gccttgaagc cgtagagggc ttcgatcttc tggcgcatct tttccgaatg gggctcggcg
      721 cccacatagg cgatgcggat cttggtatcc tttgggtcca tccggagttc ctcgaacacc
      781 gtggagaggt ggagggcgta gctggggatg atgtgaatga ccgtcgtctc gaagtccttg
      841 aggagctgga tctgacgctt gctgtttccg gccccggcgg ggatgacgag ggccccgatc
      901 cgctccgcgc cgtaatggaa gcccagaccg ccggtaaaca ggccgtaggt catcatgttc
      961 tggaagacgt cgctcttacg catccccgac atgtacatgc atcgggccag cagattggtc
     1021 cattcgctca ggtcccccgc ggtatggaag attaccgtcg cccgtcccgt cgtccccgaa
     1081 gaggaatgca tgcgcaccag ttcgtccctg ggaaccgcca cgaatccgta tggccagtgc
     1141 gcccggagat cgtccttggt ggtgggtggt attttcgtca gatcggcgcg agagcggacg
     1201 ttttccggcg ccaggccgat cttcttgaaa agctcgccgt aatagaagga tttggccgcc
     1261 catgcgaggg tgtgccgcag attctccagt tgcagcaccg ccaaattgtc cctgctgatc
     1321 gtctcgatat ctttttccca gtacatcgtt tgcctccccg tcccggcgcc ttccgaacct
     1381 gctgcgcacc gctggtttca ggcgctggtg gccgtaattt ttatgtactt tctccctacc
     1441 ccgtgtggaa aattttatca aggcaatatt gacgatgtcg atccttgcct tgacatcacg
     1501 gtggctttcc catatccttc ctcgtgcctc ggcgttcatg ggggcgtcaa tacaaattct
     1561 tcaggagggt ggcttatgtc gaaagaaatg cgcgggatgg tgatgatgtt gctggtgctg
     1621 tcggtgtcgt tcatgttctt cgtcggatgc gcgaaaccgc cgacccagga gatggggagc
     1681 gcggaaaaag cgcttacgga ggcgaaggcg aaagaggccg atctgtatgc cgccgatctc
     1741 ttcaaaaagg cggaggacgc cttgaaaaag gccaaggact acgttgccca gaagagttac
     1801 aaggaggcga aggcggcggc ggaagaggcg gcccagacgg cggggcaggc cctggcggcc
     1861 atcgagggga acaaggccaa gatgaaacag gaagccacgc agatgataga agaagcggag
     1921 aagtccctgg cggagatcaa ggcgttgacc gcagcggcga tcaaaaagaa gctcccgatc
     1981 aaccgggagg agctgcaggg gatgatcggc aaggccgaag tggatctggt cggcgtcaag
     2041 aatacgcttc aggagcagaa gatccgtccg gcctatgatc aggtcaaggc catcctggcg
     2101 cagctcaagg gcaagatcgg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 atgaaatcat cctggtcacc aatgaacccc aactgtatct cgatcaggac gtcgccatcg
     2281 tgacggacct ggaaaagggc aagggaccgc tcatggggat ctataccggc cttttctggg
     2341 ccgcctccga gaccgcgttc gttgccgcct gcgacatgcc cttcctcagc gccccgttca
     2401 tccgctatct ccgggatcag agcggcggtg atgtggatat cgtcgtcccc gaaaccgccg
     2461 acggcctcca gcccctccac gccgtctatt cccggcgctg cctgccggcg atcaaggccc
     2521 gcctggaccg ggaccgtctg aaagtcacgg agttctaccg ggggatgaag gtccggaccg
     2581 tctccgcgga aaccatccgg aggttcgacc cccacggccg gatgttcctc aacgtcaata
     2641 ccgtcgaaga gttcgccaat ctccgccaaa gtgaacagcc ctcatagacg ccactccggc
     2701 gccccggcgt accgggcggt gtggctcccg ggtcgttttc gataccgggg gctttcgatc
     2761 ctttaaagcc gcaccgtcgg ggccggttcg gggcgatcga tgcagcttag ggccggaccg
     2821 cccgtctgcc gttttcacat ggaaaatgca ttcctaccat ggaaagggac accgtcctgg
     2881 tcgtttcagc cattcttttc cgggcggcga acttccgtgc caacgtcaaa atctgttaga
     2941 ctttgagcga tgactgtgtt agtaagcccg gcattattat aaaaggagga atgtttaaca
     3001 atggcagaac agaaaacctt caacaggtgg ctggtcgtgg tcggcgcgat gctgatccag
     3061 gtcagcctcg gcgccgtcta tatctacagc gtcttcaagc ccgctctgag cgcgaaattt
     3121 cccacttgga gccccaccga cctcgccctt ccctcacagc ttatcttggc ctttttcgcc
     3181 atcggcgtca tcttcgccgg caagatccag gacaagatcg gcccccggac ggtggccacg
     3241 gtgggcggcg ccatgctggg cgtcggcctc atcatcgcct ccttcgcttc ggacctcatg
     3301 atgttcacca tcgccttcag cgtcatcggc ggtctgggga tcggcgcggc ttatgtgtgt
     3361 cccatcgcca cctgtgtgaa atggtttccc gacaagcgcg ggatgatcac cggtctcgcc
     3421 gtggccggct tcggcgccgg cgccctggtc ttcaccccca tcgccaaggc cttcatcgcc
     3481 tcttccggca tcatgtccac cttcatgtat ctgggcatca tcttcctcgt agccgtcgtg
     3541 gcgggcgccc agctcatggt cgtacccccg gcgggttaca agcctgaagg ctggaatccc
     3601 ccggccccgg ccgccaacgc cggcggcgcc aaggcggcgg gcggcgactt caccaccatg
     3661 gagatgctca agaccccgca gttctacttc ctctggatca cctatttcgc cggttgtatg
     3721 gcgggcctca tgatcatcat gaacatcacc aacatgtggc agtccccctc catgatcgaa
     3781 cttgctaaga ccatgcccac ggtcaccaaa gacgccttca gcaccgtcgc gaatcagggc
     3841 gccacggccg tcatggtcgt ggccatccta aatgccgcag gacgtctggt ctggggacag
     3901 gtttccgacg tcatcggccg caagatgacc ctcatcatca tcttcgccta tgccggggtg
     3961 atcatgctgc tcctcaacac cttcacctcc tacatgatgt tcctcgtggg cgtctgctcc
     4021 gtcggtttct gcttcggcgg tttcctgggc ctctatccgg ccgtgacggc ggactacttc
     4081 ggtacgaaga acgtgggggc caactacggc ctcatgtttg ccgcctacgg cgtcggcggt
     4141 ctcatcggtc cctggctggc cccgaagctc atgaccatcg tggggcagat cccctacgaa
     4201 gccatggaca agggcgccct gataatcaaa cagttc
//
LOCUS       DAOSMF010000151         4223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_22765_length_4223_cov_14.341651, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000151 DAOSMF010000000
VERSION     DAOSMF010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4223
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22765_length_4223_cov_14.341651"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            349..1689
                     /locus_tag="PLT63_10120"
     CDS             349..1689
                     /locus_tag="PLT63_10120"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD99118.1"
                     /translation="MDEIRKSIREIIADFQTMPLRNLTRRDMAIPVLPPGLNKVIALI
                     GMRRTGKTCLLQQIYQDLQTTQGVGRSQIIYINFEDDRLEGVIADQLRLITDTHAEMF
                     PEQAGGPRFLFLDEIQAVAGWELFVRRLVDQGDLRIYLTGSSSKMLSTEVASSLRGRG
                     LSLEVSPFSFREYLRRLAIEIPERPDSKQTALLKNRFREYLIVGGFPETIGADEELRI
                     RILQEYVLLAIHRDMVERHSIKNPEALKALIRILLEQASSLFSINKTYNGFKSQGRSV
                     SKDSLYRFIDYIQDAFLLFPVEVFSASQNRRLVNPKKIYAVDPGLVTAFSWKYARNTG
                     ALLENAVYCELRGTYPQINYYRTKSGREVDFVGTGPTGRFRLFQVCADIGDPQTLARE
                     TAALWEALDEMGIAEGVVVTLDEERRMEQAGKQIRFLPAYAWFLDLEGRKTADL"
     gene            complement(1967..2701)
                     /locus_tag="PLT63_10125"
     CDS             complement(1967..2701)
                     /locus_tag="PLT63_10125"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOD99119.1"
                     /translation="MDRLKRIDALKVRIDGHRPIDTPILKQIREYFRIGMTYSSNALE
                     GNSLTEMETKIVIEDGITIGGKPVKDHLEALGHSEAYDLLFRLAKGRYISEANIKELH
                     RLFYYRIDAKQAGKYRKQRVIITGTEFIPPVPERIPELMQSFIAGIPGAREEHHPVEF
                     AAILHWELVTIHPFIDGNGRTARLLMNLALFQAGYPPAIIPPILRRDYLDTLNKTHKG
                     DDMPFINFIVGVCYESVKEYLRLLEG"
     gene            complement(2707..3873)
                     /locus_tag="PLT63_10130"
     CDS             complement(2707..3873)
                     /locus_tag="PLT63_10130"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOD99120.1"
                     /translation="MKRLKTDYRGVYYREADRIGGKGKENVYYIVFKKDGRVHEEKVG
                     RQYADDMTPARAATIRAARIEGRRESRKDIREAAKATKLAEEGKWTLAKLWKEYSSQR
                     PDSTSVKIDGYRFQQYLEPKYGGKEPHELHQLEIDRLRINLLKQGKAPQTVKHVLGLI
                     KRLSNFGVNKQLCQGIGFKIVMPKVDNLRTEDLNPEQLKKLLAAMDASPDTEAADFMR
                     MALFTGMRRGELMNLRWKDIHFERGFILIRNPKGGKSQTIPLNDQARAVLDNHPKLAE
                     YVFVRQNKKPFTNVINKRVREIRQAAGLPSDFRPLQGLRHAYASMLASSGQVDMYVLQ
                     KLLTHKSPQMTQRYSHLRDESLKKAATLPLAGKLIIEQAAKAKARAGEKETIGA"
     gene            4041..4113
                     /locus_tag="PLT63_10135"
     tRNA            4041..4113
                     /locus_tag="PLT63_10135"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4072..4074,aa:Glu,seq:ttc)
BASE COUNT          960 a         1144 c         1025 g         1094 t
ORIGIN      
        1 tggagctttt tacttcgtcg tctccatcat gactttttac gaattcatca accttatgaa
       61 catcatttaa aaccgaggga tacgatggcg tgcgggtgaa tatcgcgccg ctgcaaaagg
      121 ctgggttatt gactgccgac gttttggcag gcaaagatgc ggacaaggcc attgccgacc
      181 gccgcctggc gcaatgacga acgccgctgg tgccgggagg gcaagctgcc ccaaccgggc
      241 tggtgggagt gacgttgttg caggcgctgt gcatgagcga cggatatttc ctttacataa
      301 ggatagattt gtaatatagc ttccttcaag caaggaaaca ttaggccgat ggacgaaatt
      361 cgcaaatcaa ttcgggaaat tatcgctgat tttcaaacca tgcctctgcg aaacctgacc
      421 cgccgggata tggcgatccc tgttttgcct ccggggctca acaaagtcat cgccctgatc
      481 ggcatgcgca gaacgggcaa gacctgtctt ttgcagcaga tttatcagga ccttcagaca
      541 acgcagggcg ttgggcgttc ccagattatc tatatcaatt tcgaagacga ccgactggaa
      601 ggcgtcatag ctgatcagtt gcggctgatt acggacaccc acgccgagat gtttcccgaa
      661 caggccggcg ggccgcgttt tctctttctg gatgaaattc aggccgtcgc cggatgggaa
      721 ctttttgtcc gccggctggt cgatcagggg gatctccgga tttacctgac cggatcatct
      781 tcaaagatgc tgagcaccga ggtggcctct tccctgcgtg gtcgcggcct gtccctcgaa
      841 gtttctccct tttcctttcg ggaatatctg cgcaggctgg ccatcgaaat cccggaacga
      901 ccggactcga aacaaacagc cctgctgaag aaccgcttca gggaatatct catcgtcgga
      961 ggatttcccg aaaccatcgg cgctgatgag gaactccgca tccgcatcct ccaggaatat
     1021 gtcctactgg ccatccacag agacatggtg gaacgccatt ccatcaaaaa tcccgaagcc
     1081 ttgaaagcgt tgatccgcat cctcctggaa caggcgtcat cccttttttc gatcaacaag
     1141 acttataacg gcttcaaatc ccagggccgg tccgtcagca aagacagcct ctaccgtttc
     1201 atcgactaca tccaggatgc ctttcttttg tttcccgtgg aggtgttctc agcgtcccaa
     1261 aaccggcgtc tggtcaatcc caaaaagatc tatgccgtcg atcccgggct tgttaccgct
     1321 ttttcctgga aatatgcacg caataccggg gcgctgcttg agaatgccgt ctattgtgaa
     1381 ctccgcggaa catacccgca gatcaactac taccggacaa aatcaggacg ggaggtggat
     1441 tttgtcggca ccgggcctac gggacgtttt cgcctctttc aggtatgcgc cgacatcggt
     1501 gacccacaga cactggctcg ggaaacggcc gccttgtggg aagcattgga cgagatgggc
     1561 atcgcggaag gcgtggttgt caccttggac gaagagcgcc gcatggaaca agcgggcaaa
     1621 cagattcgct ttctccccgc ctatgcctgg tttctcgatc tggagggaag aaaaacggcc
     1681 gacttgtagc ggagatccat tgtgaaggaa aattcattaa aaaaaggctc tgccggaaca
     1741 ggaagctgcc cggctttcca ccatcgccac gctgtcttat atgttacacc ttgcttcttc
     1801 gcccatacgc taagtttcat gggtaaatac taacacatat tgccatatat gtcagcatgt
     1861 aatggagcag ctaaataccc cccttgatta acccaggaaa acaggggagg ggttccccct
     1921 gcttgtcggc ccggttcatg acgctgtgcc tatccctggc ctgattttat ccttccaaca
     1981 gcctcaaata ttcctttacc gattcatagc aaaccccgac gatgaagttt atgaacggca
     2041 tgtcatcgcc cttatgtgtc ttattcaggg tgtccaggta gtcccggcgc aggatcgggg
     2101 ggatgatcgc cggaggataa ccggcttgaa acaaggcaag attcatcaac aaccgcgccg
     2161 tccgtccatt gccatcgatg aaggggtgaa tggtgacaag ctcccagtga aggatcgccg
     2221 caaattcaac cgggtgatgc tcctccctgg cccccggtat cccggcaata aatgactgca
     2281 tcaactccgg tatccgctct ggcaccgggg ggatgaattc cgttccggtg atgataacac
     2341 gctgcttccg atatttcccc gcctgcttcg cgtcaatgcg ataataaaac agcctatgca
     2401 gctccttaat gttcgcctcg gagatgtacc ggccctttgc caaccggaaa agaagatcat
     2461 aggcttcgga atgtcccaag gcttccagat gatccttgac cggctttccc ccgatggtga
     2521 tgccgtcctc aatgacgatc ttcgtttcca tctcggtaag cgagtttcct tccagggcgt
     2581 tgcttgaata ggtcatgccg atgcggaaat attcccgaat ctgtttgagg atgggggtgt
     2641 caatgggtct gtgtccgtcg atcctaactt tcaaggcatc gattcgtttc agtctgtcca
     2701 tgtaattcat gctcctatgg tctccttttc gcccgccctg gctttcgcct ttgccgcctg
     2761 ttcgattatc agcttgccgg caaggggtag agtagctgcc ttttttaacg actcatctct
     2821 caaatggcta tatcgctgtg tcatttgcgg cgacttgtgc gtcaacagct tttgcaggac
     2881 atacatgtca acctggcctg atgatgccag catggaagca taggcatgcc gcaacccctg
     2941 caagggccga aagtcggaag gaaggcccgc cgcttgacgg atttcccgga cgcgcttgtt
     3001 aatgacattg gtgaacggct ttttattctg cctgacaaaa acatactctg ctaacttcgg
     3061 gtgattatcc aaaacggccc gcgcctgatc gtttaagggg attgtttgac tcttgccgcc
     3121 cttggggttc cgtatcagga taaagccccg ttcaaagtga atatctttcc atcgcaggtt
     3181 catcaattca ccgcgccgca tgccggtaaa taacgccatc cgcatgaaat ccgccgcctc
     3241 tgtatccggt gaagcatcca tagccgcaag taattttttt agctgctccg ggttcaagtc
     3301 ctctgttctc agattgtcaa cctttggcat tacaatcttg aaaccaattc cctggcataa
     3361 ctgcttgttg acgccgaaat tggaaagacg ttttatcagc cccagaacgt gttttactgt
     3421 ctggggtgcc ttcccctgtt ttaagagatt gatccggaga cgatcaattt caagctggtg
     3481 aagctcgtgg ggttctttcc ctccatactt cggttccaga tattgctgga aacgatagcc
     3541 gtcgattttt acagatgtcg aatccggtct ctgactcgaa tattccttcc agagcttcgc
     3601 taatgtccac ttaccttcct ctgcaagttt ggttgccttc gctgcctccc ggatgtcttt
     3661 ccttgactcc cgacggccct cgatcctcgc cgcccggatc gttgccgccc gtgccggtgt
     3721 catatcatcg gcatattgac gaccaacctt ttcttcatga acacgcccgt cttttttaaa
     3781 gacgatataa taaacatttt ccttgccttt accgccgatc cggtcggcct cccgataata
     3841 aacgcctcga taatccgtct ttaatctctt catgttgccc tttttcaaca agccagcaac
     3901 cggatatttt ggggcatttc caaccctatt tccaaccttt ggcttaaata tagaggtatt
     3961 ttacggtatg tcaagaaaaa agatagacgt gtaaagtatt gattatgatt atgattatga
     4021 ttatgcaaat aaccgtttca gtattttatg gtaaaagggc atttctggac tttcaagcca
     4081 gtaaccgggg ttcgactccc cgtggggaca ccatatataa ataaatccac cttgaagaat
     4141 attcccttca ttttcccaag ctcggccctg tatcatggct tgttgcatat aggaccaggg
     4201 tgagggccag atcgcgactc aaa
//
LOCUS       DAOSMF010000152         4183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_23037_length_4183_cov_21.226502, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000152 DAOSMF010000000
VERSION     DAOSMF010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23037_length_4183_cov_21.226502"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="PLT63_10140"
     CDS             complement(<1..230)
                     /locus_tag="PLT63_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99121.1"
                     /translation="MVPWKEINLQRPGAGDQRCLFLADAHLRDPHETHYGVLKEFLAR
                     LAGPDTPGSGDGTSRIAVLDHLFILGDFFDFW"
     gene            412..1224
                     /locus_tag="PLT63_10145"
     CDS             412..1224
                     /locus_tag="PLT63_10145"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOD99122.1"
                     /translation="MNNYQFFEVEKIPAQQTAILYLNRPEKLNAMSWPFWRDLAPVVE
                     DLEKEPDVRVIVVAGKGKSFSVGIDVFDFFTAFSETIQGGTPEKRGEFYKLILQMQEG
                     FSRMARGDKIYIGAAHRHCIGAGLDLLSACDLRLATKDTVISLRETKIAIVADMGSLN
                     RLPRIIGQGNTRMMAYTGRDFTAAECLRMGLLNEVYDSQEDLMAGALKLAAEIADNSA
                     ATVTGTKKVLNFMEDHSVDDGLKFVAAWNSAFFNTREVQETFMKTMAKGKGK"
     gene            1760..3145
                     /locus_tag="PLT63_10150"
     CDS             1760..3145
                     /locus_tag="PLT63_10150"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HOD99123.1"
                     /translation="MAKKNPLRSFFASVKLAVVLLIVIAAASILGTLIPQQEAAGPFI
                     ARLSPGWASLLQKIQAFDVFHSFWFVTLMALLAINLIVCSLDRLPRTWRRFRSKGDPE
                     QMERFADLSADRIITTEWPRTEAADRLTSLLKKIASPVERRDRGERIFLYAHQGAFSH
                     FGVQIVHAGVLIMMAGVIVGLFLGFEGYMNIPEGESAALVSLKGGRGEKALPFSVRCD
                     RFILEFYENGAPKTYRSDLSFLQNGREVQSAAVLVNHPVTFGGIRFYQSSYGVMPGGN
                     PVLTVARRDRKIMEVEITPEMAFALPEHKATVQILRVEDNLMDMGPAAKLGVVAPTGN
                     RQFWVFAAIEEIVAANPGILEQVPLFDPASFAPYVFSLRTPERRPYTGLQAARDPGVP
                     LVALGAALLMAGLLTVYFRSHRQFWILLETAGDRTRISVAGRSSRNEAGMSRELDRLT
                     AAIGAGGEAGA"
     gene            3142..4056
                     /gene="ccsB"
                     /locus_tag="PLT63_10155"
     CDS             3142..4056
                     /gene="ccsB"
                     /locus_tag="PLT63_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HOD99124.1"
                     /translation="MIDAKILSATTFVYFAAFAFYLLRMVSSREFWGRLGSLTALTGL
                     AAQTAALALRWIASYQMGIGHAPLSNLYESLIFFSWSITAVYLFFEWRTQDKSLGAFV
                     IPAAFLFMAYASLSPHIDSRIQPLIPALQSNWLVSHVVTCFLGYAAFTVAFAAGLMML
                     LKGYLDRAGKNSRPDGAAAGAKNGCNLGTPLEYSIPARFIALIPAPAVLDDLIYQCVV
                     LGFVFLAIGIMTGSIWAHYAWGSYWSWDPKETWSLVTWLVYAIMLHLRYIRGWRERPM
                     AVMAVIGFICVLITYLGVNLLPGLHTYQ"
BASE COUNT          842 a         1267 c         1172 g          902 t
ORIGIN      
        1 aaccagaaat cgaagaaatc cccgaggata aaaagatgat cgaggaccgc gatccgggac
       61 gttccgtccc cggatcccgg cgtatccggt ccggccagtc gggcaaggaa ttccttcaag
      121 accccgtaat gggtctcgtg gggatccctc agatgggcgt cggcaagaaa caggcaccgc
      181 tggtcgcctg cccccggccg ttgtaaattg atttctttcc agggaaccat ggtttgtccc
      241 ataccagaga aatacggtat gttcaaccac cgggaaagag ttcctaccgc tggtgatgaa
      301 aacatgctat agaaagctgc ctggaagcgg cgagtgtttc gccgccggcg aaatggtttt
      361 caatggcccg gaagggacgt aacatctatt agcgaaagga gtttcccaca tatgaacaat
      421 taccagtttt tcgaagtgga aaagataccc gcccagcaaa cggcgatcct gtacctcaac
      481 cgcccggaaa agctcaacgc catgagctgg ccgttctggc gcgatctggc ccccgtggtg
      541 gaagatctgg aaaaggagcc ggacgtccgg gtgatcgtcg tggccggcaa aggcaagtcc
      601 ttcagcgtgg gcatcgacgt cttcgatttt ttcaccgcct tttccgaaac cattcagggg
      661 gggacgccgg aaaaacgagg agagttttac aaactgatcc tccagatgca ggagggcttc
      721 tcccgcatgg cccggggcga caaaatctat atcggcgccg cccatcgcca ctgcatcggc
      781 gcgggtctcg atctactgtc tgcctgcgat ctgcgtctgg cgacaaaaga caccgttatc
      841 tctctccggg agaccaagat tgccatcgtc gccgatatgg gcagcctgaa ccgtctgccc
      901 cggatcatcg gacaggggaa cacccgcatg atggcctaca cgggccggga tttcaccgcc
      961 gcggagtgcc tccgcatggg gctcctcaac gaggtgtacg actcccagga agacctcatg
     1021 gccggggcgt tgaaactggc ggcggaaatc gccgacaact ccgccgcgac cgtcacgggg
     1081 acaaagaagg tccttaactt catggaggat cacagcgtgg atgacggctt gaaattcgtc
     1141 gccgcgtgga attccgcctt cttcaatacc agagaggtcc aggagacctt catgaagacc
     1201 atggccaagg gaaagggtaa ataaaccaat gaaaacatct caggagcata tgatgagctc
     1261 tttggagcct atttctggtc tccaggatat tttcattatc cgaacgccgc gttcgcccgg
     1321 tgattcatta agacgccggt taaggcgtga atcatgccgg acggctaatc cctctgtggc
     1381 cgttattgaa ccacagagcg ccattatctt caaaaaaaag ttttatgagt ttcatattac
     1441 tattttaatc cgggatggta tgatggcgcc ccattgtccg ccgggcacca tgaaaggcct
     1501 tggaaaggag ggggcggaat cctggggagg gtttgtatct tcaaaggaac cagaaaggat
     1561 ttaggaacct cccgcccttt caccgggaat cgggaccggc ggccatggcc ggcggcaacg
     1621 taaagtgaag gtaaaacatc cggccggctc cggtattttt ttatccttta tatgataatg
     1681 cccacaaata gctggatatt ttcatctttc gttgtgcccg gaacgggcat gatggttagt
     1741 cacttacagg ggagatagct tggcgaaaaa gaatccactc cgttccttct ttgcatcggt
     1801 aaaactagcc gtcgtcctct tgatcgtcat tgcagcggcg tcgatcctgg gaacgctgat
     1861 cccccagcag gaagccgccg gtcctttcat cgcccgtctc agtcccggtt gggcatccct
     1921 cctgcagaag atccaggcct ttgatgtttt tcactccttc tggttcgtga ccctcatggc
     1981 gcttttggcc ataaacctca tcgtctgttc tctcgaccgc cttccccgga cctggcggcg
     2041 cttccggtcg aagggcgacc cggagcagat ggagcggttc gccgatctct ccgccgatcg
     2101 gatcatcacc acggagtggc cccggacgga agcggcggac cgactgacgt cgctgctcaa
     2161 gaagatcgcc agccccgtgg aacgccggga ccggggagaa cggatctttc tctatgccca
     2221 ccagggtgcc ttttcccatt tcggcgtgca gatcgtccat gccggcgtcc tgatcatgat
     2281 ggccggcgtc atagtcggtc tgttcttggg attcgagggc tacatgaaca tccccgaggg
     2341 ggaatctgcg gcgttggtat ccctcaaggg cggccgggga gaaaaggccc tgccgttctc
     2401 cgtccgctgc gaccgtttta tcttggagtt ctacgaaaac ggcgccccga agacctatcg
     2461 ctccgacctg agctttctcc agaacggacg ggaggtccaa tccgccgccg tactggtgaa
     2521 tcatcccgtc accttcgggg gaattcgctt ctaccagtct tcctacggcg tcatgcccgg
     2581 cggcaatccg gtgctcacgg tcgcccggcg ggatcgtaaa atcatggagg tggaaatcac
     2641 cccggagatg gccttcgccc tcccggagca caaggcgacg gtccaaatcc tccgggtgga
     2701 ggacaacctc atggacatgg gtcccgccgc caagttgggc gtcgttgccc cgaccggcaa
     2761 ccgccagttc tgggtatttg cggccatcga ggagatcgtc gccgccaatc ccggcatcct
     2821 ggaacaggtt cccctgttcg atcccgcctc tttcgccccc tatgtttttt ccctgcgcac
     2881 gccggaacgg cgaccctaca cgggactgca ggcggcacgc gatcccggcg tccccttggt
     2941 cgctctgggc gccgccctgc tgatggccgg tcttttgacc gtctatttcc gatcccaccg
     3001 gcagttctgg atccttctgg aaacggcggg ggatcggacg cggatcagcg tcgccggccg
     3061 gagcagcagg aacgaggccg ggatgagccg ggaacttgac aggctgacag ccgccatcgg
     3121 cgccggcggg gaggcggggg catgatcgac gccaagatcc tcagcgccac cacctttgtc
     3181 tatttcgcag cctttgcctt ttacctcctg cggatggtga gcagccgcga attctggggt
     3241 cggctgggct ccctcacggc cctgaccggc cttgccgccc agacggcggc tctcgccctg
     3301 cggtggattg cctcctacca aatgggcatc ggacatgccc ccctgtccaa cctttatgaa
     3361 tccttgatat ttttttcctg gtccatcacg gccgtctatc tgttcttcga atggcgcacg
     3421 caagacaaga gcctgggggc ctttgtcatc cccgccgctt ttttgttcat ggcctacgcc
     3481 tccctgtccc cccacatcga cagccgcatc cagcccctga tcccggccct gcagagcaac
     3541 tggctggtat cccacgtggt gacctgcttt ctgggttatg ccgccttcac cgtggccttc
     3601 gccgccggtc tcatgatgct cctgaaggga tatttggacc gggccgggaa aaactcccgc
     3661 ccggacgggg ccgcggcggg ggcgaagaac ggctgcaacc tcggcacgcc gctagaatat
     3721 tctatcccgg cccggttcat tgccctcatc cccgcccccg ccgttctgga cgatctgatc
     3781 taccagtgcg tcgttctggg cttcgtattt ctggccatcg ggatcatgac cggctccatc
     3841 tgggcgcact atgcctgggg ctcctactgg agctgggatc ccaaggagac ctggtcactc
     3901 gtaacctggc tcgtttacgc catcatgctc cacttgcgct acatccgggg ctggcgggaa
     3961 agacccatgg ccgtaatggc cgtcatcggc ttcatctgcg tcctcatcac ctatctgggc
     4021 gtgaaccttc tgccgggact gcacacctac caataaaaaa ataatgagtt cgtaaaaaac
     4081 ccgcatgtcc cgtggagcac cacgaagcat gaaaacggca ttttcatatt atcttgagag
     4141 tccataattt tatgtaattt atgccaccat tggcaattga tgc
//
LOCUS       DAOSMF010000153         4176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_23086_length_4176_cov_18.939578, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000153 DAOSMF010000000
VERSION     DAOSMF010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23086_length_4176_cov_18.939578"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..890
                     /locus_tag="PLT63_10160"
     CDS             78..890
                     /locus_tag="PLT63_10160"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOD99125.1"
                     /translation="MPLNIIVCIKQVPNPEHFDKITLDPSTGTIRRAGIPVITNPLDR
                     HALEEALRIREKFGGAVTAITMGPLQARKSLDDALSMGADRGALLCDQMFAGADTLAT
                     ACVLSGGIRLLGPFDLILCGNESADGATAQVPAQLAEMLDLPHVTCARKIDMSADGSK
                     ALVERAVEHGFLRVEVVLPAVIAVLKTINTYRLPTVLGIMEAAGKEVHQLGCSACEST
                     GMYPADIGSIGSPTRFAGISEAAHLRKVKMITGDPQDIAAQLIRELRYRDAL"
     gene            905..1900
                     /locus_tag="PLT63_10165"
     CDS             905..1900
                     /locus_tag="PLT63_10165"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOD99126.1"
                     /translation="MGDIIVWAEHRDGKLVSVSLELMAKARELADRIEGHLTAILIGD
                     RCGHLAETLLHYGASRVFIMEDPRLALYQSDLYPRLMASLLKDLSPEIVLLGATSIGA
                     DLAPRVAAKLKTGLTAHCVDLYIENIAGTNQLIQVVPGWSGNMLIKIICPERRPQMAT
                     VRPDVLEIGKADPSRSGDIIRIQPEFAEHEMKAKTIEFVREESEGSSITDADVIVSGG
                     FGLESAGGFTLINELAQAVHGEVAGTRPAFDRGWIPEARMIGQSGKTVKPRIFISIGT
                     SGATHYTTGFLKAKTIVAIDRNPRAPIFGISDLGIVGDLREIIPCLIDELKNDVR"
     gene            1971..3098
                     /locus_tag="PLT63_10170"
     CDS             1971..3098
                     /locus_tag="PLT63_10170"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOD99127.1"
                     /translation="MFELTKEQEMLRAMVREFADTELAPKALELDRKGDFPFEIAKKM
                     AALGMLGISTSKELGGSGMGHLAGTIAIEELARVYPSVAFFMEVSTGPLYAITNFGSV
                     EQKKKYAPLILKGEKIMCFAATEPSGGSELANLGTEAVPTGEGYIINGRKVYITGGGI
                     ADYCVLLAKTGDRASIFLIEQGTPGFTVGRRQEQIGLKSVDVSELAFNDCKIPKENII
                     GKEGAGFPIAMTTFTVARPAIGAIGLGIARGAFEIALKYAKERILYGKPIARLQAIQL
                     LLADMDTEIDAARWLVYAPGAALDKGATPRDIVKQSSRAKTVGAEVGFSVTRKAIEIL
                     GGNGVSPEYHLGRLLNDAVELIPATGTQQIMKIIQAGEILK"
     gene            3188..4096
                     /locus_tag="PLT63_10175"
     CDS             3188..4096
                     /locus_tag="PLT63_10175"
                     /inference="COORDINATES: protein motif:HMM:NF004283.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HOD99128.1"
                     /translation="MYIPESVEINEVVLRDGIQNDKKIVPTDDKVRLVHDLAACGIRR
                     MEISSFVNKKLVPQMADAEELWERIERKKDVIYSALILSEKGLDRAIRCRVPHVSFFV
                     SASETHSIKNSNKTVEEAMKEALRLIGKARDAGMGVRAGVMNTFGCAYDGKVPVSAVL
                     KHVRAFMEREPDEIGLADTTGMANPRQSAELLKLVKDVTGDTPLSVHFHNTRDLGLAN
                     VWAAINEGVTIFDSSLGGLGGCPFIPGAKGNIATEDFLHMMHEMDILTGVDLNRFIDV
                     SLGFEKVMGQTFPAFVTHLQKAACGC"
BASE COUNT         1040 a         1049 c         1190 g          897 t
ORIGIN      
        1 agcatcctca ccctatcgta cgtttgcatt gacaaggtat tttttatctg gtaattttcc
       61 gcgcgggaaa tacggagatg ccactgaata tcattgtttg cattaaacaa gtcccgaatc
      121 cggaacattt cgataagatc acccttgatc cctctacggg aaccattcgc agggctggga
      181 ttcctgtcat cacgaatccg ctcgaccggc atgccttgga agaagcccta cgcatcaggg
      241 aaaaattcgg cggtgccgtc acggccatta ccatgggccc tctccaagct cgcaaaagtc
      301 ttgatgatgc cctgtccatg ggtgcggatc ggggggcgtt actctgcgac caaatgttcg
      361 caggagcgga tacgttggcc acggcctgtg tcttatcggg gggcattcgt cttctagggc
      421 cctttgacct gatcctgtgc ggaaatgaat cggccgatgg ggcgacagcc caggttccgg
      481 ctcaacttgc agagatgctt gaccttcctc atgtaacctg cgcgcgaaaa atcgacatgt
      541 ccgcggacgg cagcaaggcc cttgtggaaa gggcggttga acacggcttt ctgagggtgg
      601 aagtggtcct gccggcagtg atagccgttc tcaaaacgat caatacctac cgcttgccca
      661 cggtactcgg aatcatggag gcggcaggaa aagaggttca ccaactgggc tgttccgcct
      721 gcgaatccac cgggatgtat cccgccgata tcggctcaat cggatcaccc acgcgtttcg
      781 cgggcatctc cgaagccgca caccttcgga aggtgaagat gattaccgga gatccgcaag
      841 atatagctgc gcagctcatt agagaactgc gttataggga tgcgctgtag gcgaagaggt
      901 tgttatggga gatatcatcg tctgggccga acatcgggac ggaaaactgg tctccgtttc
      961 cctggaactg atggccaagg cccgggagct cgcggatcgg attgagggcc atctcactgc
     1021 tattctgatc ggtgatcgat gcggtcatct tgcggaaacg ctgctccact acggcgcctc
     1081 cagggtcttc atcatggagg atccccgcct ggccttatac cagagcgatc tctaccccag
     1141 attaatggcc agtttgctga aagacctctc gccggagatt gtgcttctgg gcgcaacatc
     1201 catcggggcg gatcttgctc caagggtcgc ggcaaaactg aagacggggc ttacggcaca
     1261 ctgtgttgat ctttacatcg aaaacatcgc cgggacaaat cagctcatcc aggtggtgcc
     1321 gggttggagc gggaacatgc tcatcaagat catctgcccc gagcggcggc cccagatggc
     1381 caccgtgcgc ccagacgtcc tggaaatagg aaaagctgac ccgtcacgct caggggacat
     1441 tatccgtatc cagcccgaat ttgcagagca cgaaatgaag gccaagacaa ttgaattcgt
     1501 ccgcgaggaa tccgaaggca gctccataac agatgccgat gttattgtat cgggtggctt
     1561 cgggctcgaa tcggccggcg gattcacact tataaatgaa ttggcacaag cggttcacgg
     1621 cgaggtggcg ggaacgcgcc ccgcctttga tcggggctgg ataccggagg ccagaatgat
     1681 aggacagagc ggaaaaacgg tcaagccgag gatttttatc agcatcggca catcgggagc
     1741 gacacattac acgacgggat tccttaaagc gaagaccatc gtcgccatcg accgcaatcc
     1801 acgggcaccg atcttcggca tatcggatct cggcattgtg ggagatttga gagaaatcat
     1861 cccctgcctg atcgatgagc ttaagaacga tgttcgatga attgcccgac gacaagttcg
     1921 gagaatagca ttcataggga tcaacaatac gaagggaggg gaaatgacca atgtttgagt
     1981 taacgaaaga gcaagagatg ttgagggcga tggtacgaga atttgcagat actgaactgg
     2041 cacccaaggc gctcgaattg gataggaaag gtgattttcc attcgaaatc gccaaaaaaa
     2101 tggccgctct cggcatgctt ggcatatcaa cgtccaaaga gcttggcggc tcggggatgg
     2161 gtcacctggc gggaacaatc gccattgaag aacttgcccg cgtgtatcct tcagtggctt
     2221 tcttcatgga ggtcagcacg ggtcccctgt atgccatcac gaattttggt tcggtggaac
     2281 aaaagaagaa atatgcaccc ttaatcctta agggggaaaa aatcatgtgc tttgccgcta
     2341 cggaacccag tggtggatcc gaattggcta accttggaac ggaagcggtg ccgactggag
     2401 aaggctatat aatcaacggc cgaaaggttt atatcaccgg ggggggcatc gcagattact
     2461 gtgttctgct cgcaaaaacc ggtgataggg caagcatctt cctgatagaa cagggaacgc
     2521 cagggtttac agtcggcagg aggcaggaac agattggact gaagtcagtg gacgtaagcg
     2581 agcttgcctt taatgactgt aaaatcccga aggaaaacat catcggaaag gaaggcgcgg
     2641 gctttcccat cgccatgaca acctttacgg tagcaaggcc ggctattggg gcaattggcc
     2701 tcggcatcgc ccgcggcgcc tttgagatcg cactcaaata tgccaaagag cgcattcttt
     2761 acggaaagcc catagcgagg ctgcaggcca ttcaactcct cctggccgat atggatacgg
     2821 agatcgatgc agcccggtgg cttgtttacg cacctggagc cgccttggac aaaggcgcaa
     2881 ctcctcggga tattgtaaaa caatcatcac gcgccaagac ggtgggagcg gaagtcgggt
     2941 tcagtgtcac ccgtaaggca atcgagattc ttggaggaaa cggtgtcagt ccggaatatc
     3001 accttggccg gcttttgaat gatgctgtgg aactcatccc tgccaccgga acgcaacaga
     3061 tcatgaagat catccaggcg ggagaaattt tgaaataagt aaaaattgaa ggcaagttag
     3121 aatgttgctg taatatagat cgtatttgtt accctgatag ataaaatcga atcaattggg
     3181 aatggaaatg tatatacctg agagcgtgga aatcaatgaa gtcgtcctcc gggacggaat
     3241 ccagaacgac aagaagatcg tccctacaga tgacaaggtc cgcctagtcc atgatctcgc
     3301 cgcctgcggc ataaggagga tggagatttc gtcattcgtg aacaaaaaac tcgtacctca
     3361 gatggccgac gcggaggaac tctgggaacg catcgagagg aagaaagacg tcatttattc
     3421 agcgttgatt ttaagcgaaa agggactgga cagggccatc cgctgccggg tcccccatgt
     3481 gagtttcttc gtgtcggcca gtgagacaca cagcatcaag aacagcaaca agacggttga
     3541 ggaagctatg aaggaggcgc tccgtcttat cgggaaggcc cgcgacgccg ggatgggagt
     3601 gcgggcgggc gtcatgaata ccttcggatg cgcctacgac gggaaggttc ccgtgagcgc
     3661 agtcctgaag catgtccggg ctttcatgga aagagaaccc gatgaaatcg gccttgccga
     3721 cacgaccggc atggccaatc cccgccagtc ggccgaactt ttgaaactgg tcaaggatgt
     3781 gacgggcgac acccccctgt cggttcattt ccacaatacc cgtgacctgg ggctggccaa
     3841 cgtctgggca gcaatcaatg agggggtgac gatctttgat tcatccctgg gagggctggg
     3901 tggctgcccg tttatccccg gcgcaaaagg caatatcgca acagaggatt ttcttcacat
     3961 gatgcatgag atggatatct tgacgggtgt cgatctcaac cgtttcatag atgtcagcct
     4021 cggtttcgag aaggttatgg ggcagacctt cccggccttt gtcacccacc ttcaaaaagc
     4081 cgcttgtgga tgttgagtgg tgaccatccc accacatccg gcggatcttg ttatcaatgt
     4141 cgatatttta tagcgcccag gtcgatttac cttatt
//
LOCUS       DAOSMF010000154         4167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_23157_length_4167_cov_23.878405, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000154 DAOSMF010000000
VERSION     DAOSMF010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23157_length_4167_cov_23.878405"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..88)
                     /locus_tag="PLT63_10180"
     tRNA            complement(2..88)
                     /locus_tag="PLT63_10180"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(51..53),aa:Leu,seq:gag)
     gene            complement(218..592)
                     /gene="secG"
                     /locus_tag="PLT63_10185"
     CDS             complement(218..592)
                     /gene="secG"
                     /locus_tag="PLT63_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOD99129.1"
                     /translation="MHTLIIILHIIICIALILVVLLQAGKGANMGAVFGGSSQTIFGS
                     SGPGTFLGKMTTAIAVIFMLTSLGLSYLSVQKGSALMKGSVGRPAAERSLPAAPPVPI
                     AAAPIPAPAAKQPVTDPVQKSR"
     gene            complement(598..1359)
                     /gene="tpiA"
                     /locus_tag="PLT63_10190"
     CDS             complement(598..1359)
                     /gene="tpiA"
                     /locus_tag="PLT63_10190"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOD99130.1"
                     /translation="MSRSSLIAGNWKMFKTPGEAVEFARLLVAELQVPTDREVVIAPA
                     FPSLTAVAAVLRGSEIALAAQNVHQEPQGAFTGEVAAGMLRDAGCRYVIVGHSERRTY
                     FRETDELINRKLAAALASGLKPILCIGETLTEREQDKTLEVIARQLKEGLKNVAPDDI
                     GSAVVAYEPVWAIGTGRTASPGQAQEVHAFIRDELGGKYGPAVARRLRIIYGGSVNPV
                     NMGKLMAEKDIDGALVGGASLEVASFSQIVNYPEV"
     gene            complement(1366..2373)
                     /gene="gap"
                     /locus_tag="PLT63_10195"
     CDS             complement(1366..2373)
                     /gene="gap"
                     /locus_tag="PLT63_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011897301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOD99131.1"
                     /translation="MGIKVAINGFGRVGRYLVRACHGHEDIEIVAVNSRAKPAVLAHL
                     LKYDSVHGIFAADIAATENELLINGKKIAITQVTSTLADLPWAALGVDIVLESTGKFR
                     KKDEVAGHLQAGAKKIIIAAPGKGIDGTFVMGVNEHTYDAKKHHIISNASCTTNCLAP
                     VVKVLHDEFTVVRGLMTTVHAYTMDQRILDGSHKDLRRGRAAALSIVPTSTGAARAVT
                     EVIPDLKGKMDGVALRVPTPNVSIVDLAAELKRNVTIAEINGALKAAAEGKLKGILAY
                     CDEELVSVDFTSSPYSSIVDGPLTNLVDGNFVKVFSWYDNESGYACRMRDLALYIGKQ
                     L"
     gene            complement(2518..3186)
                     /gene="nth"
                     /locus_tag="PLT63_10200"
     CDS             complement(2518..3186)
                     /gene="nth"
                     /locus_tag="PLT63_10200"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOD99132.1"
                     /translation="MNIKRRDRDIGVVVRRLQETILAEGVPIVSRMAAEGYSPFAILA
                     STLLSLRTKDEVTAAATDRLLKLADTPGKMMHLPEAAIRKAIYPVGFYRNKARRLREI
                     SATLVENYGGTVPDSLEELLKLKGVGRKTANLVLSLGFDKDGICVDTHVHRISNRLGY
                     VATRTPEKTEAALREKLPRKYWKIYNTILVAFGQKLCRPISPLCSACPVRDCCDRIGV
                     TRSR"
     gene            complement(3355..>4167)
                     /locus_tag="PLT63_10205"
     CDS             complement(3355..>4167)
                     /locus_tag="PLT63_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HOD99133.1"
                     /translation="KTIEFYKNQNPHYEELLAILEEILILREKYRRKLKTDVFPVDER
                     LIPSKIEGGLPLIDLSQGDYDLTLPREYFLDLLHIAEKRAPGETEELARKINDGEEDF
                     RELVLGAFYGEPGEMEDDEQDNEEETFDLIDLFLEECLRPAFELVAAKYGEAVLKSKW
                     SEGYCPICGKEPKIGEIKEEEGRRYLFCNQCGIKWPFLRIKCPFCGNEEQQTLAYFTV
                     EDDERYRVDVCNECKRYIKIVDFRETKEEANLDIEDITTLHLDMLATEEGYD"
BASE COUNT          759 a         1213 c         1230 g          965 t
ORIGIN      
        1 atggtgccga agccgggact tgaacccgga cgggcgtggc ccactacccc ctcaagatag
       61 cgtgtctacc agattccacc acttcggcgc tgttgactag atccctggcc cttcctctac
      121 atcctcacgt gttttttgtc aatacgaaaa cattcctgtc gggcaggttt tccctgtgcc
      181 ctggtcattt tggggccctt tcggtctcac ggcctcttta gcgtgacttc tggacggggt
      241 ccgtcacggg ctgtttggcg gctggcgccg ggataggcgc cgcagcgatg ggcaccggcg
      301 gggctgccgg cagcgatctc tcggcggcgg gcctcccgac actgcctttc atcagggcgc
      361 ttcccttctg tacggacagg taggataagc ccagtgaagt gagcatgaag atgacggcga
      421 tggcggtagt catcttgccg aggaaggttc cggggccgct gctgccgaag atggtctggc
      481 tggaaccgcc gaagaccgcc cccatgttcg cccccttgcc ggcctggagg aggacgacga
      541 ggatcagggc aatgcagatg attatgtgta ggattatgat cagggtgtgc atgttttcta
      601 tacctctgga tagttaacga tttgtgaaaa tgaggctacc tcgagactgg cgcctccgac
      661 aagggcaccg tcgatgtcct tttccgccat gagctttccc atattgacgg gattcacgct
      721 cccgccgtag atgatccgga gccgccgggc gaccgctggc ccgtatttcc cgccgagctc
      781 atcccgaatg aaggcgtgca cttcctgggc ctgccccggc gaggccgttc tccccgttcc
      841 gatcgcccag accggttcat aggcgacaac ggcgctccct atatcatcag gggcgacatt
      901 cttcaatcct tcttttagtt gccgggcgat tacctccaag gtcttgtcct gctccctttc
      961 ggtcagcgtc tcgccaatgc agaggatggg cttcaatccc gaggccaagg cggcggcaag
     1021 cttgcgatta atcagctcat cggtttcgcg aaagtaggtc cgccgctccg agtggccgac
     1081 aatcacatag cggcaccccg catcacggag catccccgcc gccacttcgc cggtgaatgc
     1141 cccctggggc tcctgatgga cgttctgggc ggcgagcgcg atttccgatc cccgcaggac
     1201 tgcggcgacc gcggtgaggg aaggaaaggc gggggcgatg accacttccc ggtccgtcgg
     1261 cacttgcagc tccgccacca ggaggcgggc gaattccacc gcctctccgg gggttttgaa
     1321 catcttccag ttgccggcaa tcaacgatga tctggacatg ttcacctaca gttgtttgcc
     1381 tatatagagg gccaggtctc gcatccggca ggcgtagccg ctttcgttgt cataccagga
     1441 gaagaccttg acgaaatttc cgtccaccaa gttggtcaga gggccgtcca cgatggagga
     1501 gtaggggctg ctggtgaagt ccacggagac gagttcctca tcgcagtatg ccaggatgcc
     1561 tttcagcttt ccttccgccg ccgccttcag cgcgccgttg atctcggcga tggtcacgtt
     1621 ccgtttcaat tccgcggcca gatcgacgat ggatacattg ggggtgggca cccggagcgc
     1681 caccccgtcc atcttgccct tcagatccgg gatgacctcg gtgacggccc gggcggcccc
     1741 cgtcgaggtc gggacgatgg aaagagccgc cgctcttccc cggcggaggt ccttgtggga
     1801 gccgtcgagg atgcgctgat ccatggtata ggcgtgaaca gtggtcatca ggcctcttac
     1861 caccgtgaat tcatcatgga gaaccttgac gactggggcc agacagttgg tcgtgcatga
     1921 ggcattggag atgatgtggt gctttttggc gtcgtaggta tgctcgttga cgcccatgac
     1981 gaaggtgccg tcaataccct tgcccggggc ggcgatgatg atctttttcg ccccggcctg
     2041 aaggtgtccc gccacctcgt ccttcttgcg gaacttcccc gtggattcca gcacgatatc
     2101 cacccccagg gcggcccagg gaaggtccgc cagcgtggag gtcacctggg tgatggcgat
     2161 cttcttgccg ttgatcagga gttcgttttc cgtggcggcg atgtcggcgg cgaagattcc
     2221 gtgcacggag tcgtatttca acagatgggc cagtaccgcc ggttttgccc gggaattgac
     2281 ggcgacgatt tcaatatctt catggccgtg acaggcccgg accagataac gacccactct
     2341 tccgaaacca ttgattgcga ctttaatgcc catttataaa cctcccttat gctcagccat
     2401 aggcaatggc gttgttttca ggccgtgata ggacggtgcc cttataaccg tagagagacg
     2461 tttagtcaac tggaattatt atgctaataa ttttgaatgg taacgcggcg gggattgtta
     2521 gcggcttctg gtcacgccga tccggtcgca gcagtcgcgg accgggcagg cgctgcacag
     2581 gggggagata ggccggcaga gtttctgtcc gaaggccacg aggatggtgt tgtatatctt
     2641 ccagtatttc ctgggcaatt tctcccggag ggcggcctcg gtcttttccg gggtcctggt
     2701 cgccacatat cccagccggt tggagatgcg gtgcacatgg gtgtccacgc agatcccgtc
     2761 cttgtcgaag cccagggaca gaaccaggtt tgccgtcttc cggcccactc ccttgagctt
     2821 caaaagctcc tccaggctgt ccgggacggt gcccccgtag ttctcgacaa gggtggcgct
     2881 gatttcccgg aggcggcgcg ccttgttgcg ataaaagccc accgggtaaa tggcctttct
     2941 gattgccgct tccggcagat gcatcatttt tccgggggtg tccgccagct tcagcagccg
     3001 gtcggtggcg gcggcggtaa cctcgtcctt ggtccggagg ctcagaagcg ttgaggcgag
     3061 gatggcgaag ggggagtacc cttccgcggc catgcgggaa acgatgggaa cgccctctgc
     3121 cagtatcgtt tcctggaggc gccgcactac cacgccgata tcccgatccc gccttttgat
     3181 attcacgccc ggcgacgcat tttgatcaag gtcataaaca gcattgatca taatatacac
     3241 caatcgccgc cgccaggttg gtcgtccaaa ctcgttgggc cgggagacct acgggcaccc
     3301 gtggccgtgg ggggacgccc cggtcgggta aacggcggca ccggctttag ggcttcagtc
     3361 gtagccttcc tccgtggcca gcatgtccag atggagggtg gtgatgtcct cgatatcgag
     3421 gttggcctct tccttggttt cccggaaatc gacgatcttg atataccgct tgcattcatt
     3481 gcagacatcg acacggtacc gctcatcgtc ttccacggtg aaataggcca gggtctgctg
     3541 ttcctcattg ccgcagaaag ggcatttgat tctcaggaat ggccatttga tgccgcactg
     3601 gttgcagaag aggtagcgcc ttccctcttc ctctttgatc tcgcctatct tgggttcctt
     3661 gccgcagatg gggcaatatc cctccgacca tttggacttg agtaccgcct ccccatattt
     3721 ggccgctacc agttcgaaag cgggacggag gcattcttcc aggaaaagat caatcaaatc
     3781 aaaagtttct tcttcattgt cctgttcgtc atcctccatt tctccaggtt cgccgtagaa
     3841 ggcgcccaag acaagttccc gaaaatcctc ctcaccgtca ttaatttttc tcgccagttc
     3901 ctcggtttcc cccggggccc gcttttcggc gatgtggagg agatcaagaa aatattcccg
     3961 gggtagagtt agatcgtagt ctccctggga taggtcgatc aggggcagtc ccccttcgat
     4021 cttggaaggg atgagccgtt cgtccacggg aaagacatcc gtcttaagct tgcgccggta
     4081 tttctccctg aggatgagga tctcttccag gatggcgagg agttcctcgt aatggggatt
     4141 ctggttcttg taaaactcaa tggtttt
//
LOCUS       DAOSMF010000155         4162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_23196_length_4162_cov_17.177015, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000155 DAOSMF010000000
VERSION     DAOSMF010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4162
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23196_length_4162_cov_17.177015"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(301..852)
                     /locus_tag="PLT63_10210"
     CDS             complement(301..852)
                     /locus_tag="PLT63_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HOD99134.1"
                     /translation="MTLLLHICCAPCTIYPLRVLREEGFDLTGFYCNPNIHPYREYMR
                     RWEALDGFAREERLKIRAYPNYEMEAFLRTVVFKEVERCRWCYELRLRQTADAARKGN
                     YGGFSTTLLYSKYQKHELIRSVGEQLAQEYAVPFLYRDFRLGWEEGVRISRERQIYRQ
                     PYCGCIYSEKERYAKKRPVSSEE"
     gene            complement(987..2018)
                     /gene="ruvB"
                     /locus_tag="PLT63_10215"
     CDS             complement(987..2018)
                     /gene="ruvB"
                     /locus_tag="PLT63_10215"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOD99135.1"
                     /translation="MKPTKNNLVLDPDAGREDQAFDVSLRPKALAEYVGQEKIKNNLA
                     IFIEAAKTRGEALDHALLYGPPGLGKTTLAYIMAKELGVDVKVTSGPVIERPGDLAAI
                     LTNLHDHDILFIDEIHRLSHVVEEILYPAMEDYHIDILIGQGPSARSMKLGIPRFTLV
                     GATTRAGLLTSPLRDRFGMSFRFDFYNPVELTVIIRRSAHILAVDIDEEGAKEMARRS
                     RGTPRIANRLLRRVRDYAQVKARGAINKEVALAALELLEVDHRGFDQMDRTILLTLID
                     KFQGGPVGIDNLAAAIGEEKTTLEDVYEPYLIQEGYLQRTSRGRIATRAAYEHFGRSW
                     TGGNQKDLF"
     gene            complement(2049..2663)
                     /gene="ruvA"
                     /locus_tag="PLT63_10220"
     CDS             complement(2049..2663)
                     /gene="ruvA"
                     /locus_tag="PLT63_10220"
                     /inference="COORDINATES: protein motif:HMM:NF001040.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOD99136.1"
                     /translation="MISRLSGILAYKSLPAVTVDVHGVGYRVFVPLTTFYELPEPGKS
                     ITLQLHTHVKEDAISLFGFYTLEERDMFQVMISVSGIGPRLALNILSGISAEELVDAI
                     TGGNLRRLLRIPGVGKKMAERLVLELKDKVLKLERRQEAARERAVPRSAADAVKEDAL
                     SALINLGYKNALAREVIEKIIDEEQPVVPSLDVLLKKALQRLSG"
     gene            complement(2660..3142)
                     /gene="ruvC"
                     /locus_tag="PLT63_10225"
     CDS             complement(2660..3142)
                     /gene="ruvC"
                     /locus_tag="PLT63_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOD99137.1"
                     /translation="MRILGIDPGSRITGYGIVEKSRHGLTGGDYGEIKPPRNASLATA
                     LVYVYESLTGLIAALQPNAVAIEDIFYGKNVQSLIKQGHVRGVAILAGARRDVLVYEY
                     SPLEVKKAVVGYGRAEKSQVQTMIKAILNLSESPPPDAADALAVAVCHAHSTPAVVAS
                     "
     gene            complement(3142..3891)
                     /locus_tag="PLT63_10230"
     CDS             complement(3142..3891)
                     /locus_tag="PLT63_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOD99138.1"
                     /translation="MSGHNKWSTIKHKKGAADAKRGKIFTKIIKEITLAARLGGSDPD
                     GNPRLRTAILAAKDENMPKDNIERAIKKGGGTGADAVNYEEITYEGYGPGGVAVLVEV
                     MTDNKNRTVAEIRHIFSKHGGNLGENGCVSWMFDKKGSILIDKKAIDEEELMELALEA
                     GADDVREEEQEYEIITSPAVFEEVHRAVEAKGLKPLAAQIGMVPQNTIALGEDKAPQM
                     LKMMEKLEDNDDVQNVYANFDISDEMMEKLS"
     gene            complement(3946..>4162)
                     /locus_tag="PLT63_10235"
     CDS             complement(3946..>4162)
                     /locus_tag="PLT63_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99139.1"
                     /translation="RDYLKNPFACEGVFYFTCTPTCVIKVRLCTNHNAFVQMEEEELI
                     NTPKNILPCRSKAGGHGIPGSGFHNPP"
BASE COUNT          887 a         1203 c         1064 g         1008 t
ORIGIN      
        1 gaaggatcgt cattccgacc tttcttcccg tatggaggcc gatgctccga cagttgatct
       61 cattcctcag ggttctttgc cggtacatta gttcactctc acaggtcttt cttcagactg
      121 tctttcgggc tgatatagag caatatccgt acctagttaa ccaatacaga tacagaaaaa
      181 tatttccttc aatatcaagg atttcgcaaa tgcaaccccg gtcagcctca atacgaacgc
      241 cgatgctgtg tcaataacgc cacaatgcct gcacctgctg ggaaaggaga gcaaaaagag
      301 tcattcttcg ctgctcaccg gccgtttttt cgcgtacctt tccttttcac tgtagatgca
      361 gccacagtat ggctggcggt aaatctgtct ctcccgggaa atccgcaccc cttcttccca
      421 gccaaggcga aaatcacgat aaagaaaagg aacggcatat tcctgggcaa gctgttcccc
      481 gacactgcgg atcagttcgt gtttctgata cttgctgtat aaaagagtcg tggaaaaccc
      541 gccatagttc cctttccggg cggcatccgc cgtctgcctc aggcgaagct cgtaacacca
      601 gcgacaccgt tcaacctcct tgaagaccac cgtccgcaaa aaggcctcca tttcataatt
      661 gggataggct ctgatcttca gacgttcctc ccgagcaaaa ccgtccaggg cttcccaacg
      721 gcgcatatat tcacggtagg ggtgtatgtt ggggttacag taaaaccccg tgaggtcgaa
      781 accctcttcc ctgagaaccc gcaatggata gatggtacaa ggggcgcaac aaatgtggag
      841 cagcaatgtc atgatggctg tccgagaaag cgaagcttag tgttcataaa gcgaagctta
      901 gtattcataa aagggagagc taatatactg ctcatcccca tttgaattta tggtgatctg
      961 tttctggcgg tcctgaccgg aggcaatcag aacaggtcct tctggtttcc ccccgtccaa
     1021 gagcgaccga aatgctcata ggccgccctg gtggcgatcc ggccccggga ggtccgctgg
     1081 agatagcctt cctgaataag atacggctca taaacatcct ccagagtagt tttctcttcc
     1141 cctatcgccg ccgccaggtt atcgatccct accggccccc cctggaattt gtcgatcagc
     1201 gtcagcagga tcgtccggtc catctggtcg aaaccccgat gatccacttc cagaagttcc
     1261 agcgccgcca gggccacctc cttgttgata gcaccccggg cctttacctg ggcgtagtcc
     1321 cggaccctac gaaggaggcg gttggcgatc cgcggggtgc cccgggaacg gcgggccatt
     1381 tccttcgccc cctcttcgtc gatatccacg gccaggatat gcgcggatct ccgaatgatc
     1441 accgtcagtt ccacgggatt gtagaaatcg aaccggaaac tcatcccgaa gcggtcccgg
     1501 aggggcgagg tgagaaggcc ggcccgggtc gtcgcgccca ccagggtaaa ccgggggatg
     1561 cccagtttca ttgagcgagc cgatggaccc tgaccaatga ggatgtcgat gtggtaatct
     1621 tccatggccg gatagaggat ctcctcgact acatgggaaa gacggtggat ttcgtcaatg
     1681 aagagaatgt cgtgatcatg aaggttggtg agaatcgccg ccaagtcgcc ggggcgctcg
     1741 ataaccggtc ccgatgtcac cttgacgtcc acaccgagct ccttggccat gatgtaagcc
     1801 agggttgtct tgcccagccc gggcgggccg tagagaaggg catgatccag tgcctctccc
     1861 ctggttttcg ccgcctcgat aaagatggca aggttgtttt tgatcttctc ctgaccgaca
     1921 tattccgcca gggccttcgg tcgcaacgag acgtcgaaag cctgatcctc cctgccggca
     1981 tcgggatcca aaaccagatt atttttggtc ggcttcaaaa gctgcgcatc cttcccatcc
     2041 tcggaaactc agcccgacaa cctctggaga gcctttttca aaaggacatc cagggaagga
     2101 accacgggct gctcctcgtc aatgatcttt tcgataacct cccgagcaag ggcgttttta
     2161 taacccagat tgatgagcgc cgaaagggcg tcctctttca cggcgtcggc ggccgaacgg
     2221 ggaacggcgc gctcccgggc ggcttcctgt ctccgttcca gtttcagtac cttgtccttg
     2281 agttccagca ccagtctttc cgccattttc ttccccactc ccgggatacg caagaggcgc
     2341 ctgagatttc cgccggtaat ggcatccacc agttcctcgg cgctgatgcc tgaaaggata
     2401 ttcaaggcca gtcggggacc gatcccggaa acggagatca tgacctgaaa catgtcacgc
     2461 tcctccaacg tatagaaacc aaagaggctg atggcgtctt ctttcacatg ggtatgcagc
     2521 tggagggtta tcgacttgcc cggttccggc aattcgtaaa aggtcgtcaa agggacgaaa
     2581 actcgataac cgacgccgtg gacatctacg gtaacggcgg ggagggactt gtaagccagg
     2641 atgccgctga gccgggagat catgacgcaa ccacagcggg agtggaatgg gcgtggcaga
     2701 cagctacggc cagggcgtcg gcggcgtcgg gagggggaga ctccgacagg ttgaggatgg
     2761 ctttgatcat ggtttgcact tggctctttt ccgctcgtcc ataaccgaca acggccttct
     2821 ttacctccag cggcgaatat tcatacacaa gcacatcccg ccgggctccg gccagaatgg
     2881 ccactccccg gacatgtccc tgtttgatca ggctttgcac gtttttaccg tagaagatat
     2941 cctcgatcgc cacagcgttg ggttgcaacg ccgctatcaa gccggtgagg ctttcataaa
     3001 cgtaaaccag ggccgtggcg agagaagcgt tccggggcgg cttgatctct ccgtagtctc
     3061 cacccgtcaa tccatgacgg cttttttcga cgatcccgta accggtgatc ctgctgcccg
     3121 gatcgatgcc tagaatcctc atcagctcaa cttttccatc atctcgtcgg aaatgtcgaa
     3181 gttggcgtaa acattctgca cgtcatcatt atcttcgagt ttctccatca tcttgagcat
     3241 ctggggagcc ttgtcctccc ccagggcaat ggtgttctgg ggaaccattc cgatctgagc
     3301 cgccaggggc ttgagtcctt tggcttcgac cgcccggtgg acctcctcga aaaccgctgg
     3361 actggtgata atctcgtatt cctgttcctc ttcccggaca tcgtcagccc cggcctccag
     3421 ggccagttcc atcagttcct cctcatcgat tgccttcttg tcgatgagaa tgcttccctt
     3481 tttatcgaac atccaggaca cgcaaccgtt ctcaccgaga ttgccgccat gtttcgagaa
     3541 gatatggcgg atctccgcca ccgtcctgtt tttgttgtcc gtcatgacct ccaccagaac
     3601 ggctaccccg ccgggtccat agccctcgta ggttatctcc tcgtaattga cggcatcggc
     3661 gccggtaccg ccacccttct tgatggcccg ttcgatgttg tccttgggca tgttctcgtc
     3721 tttcgcggcc agaatcgccg tccgtaggcg tggattcccg tccggatcgg atcctcccaa
     3781 gcgggccgcc aaggtaattt cctttattat tttcgtgaat atcttgcccc gtttcgcatc
     3841 ggcggcgcct tttttgtgtt ttatggtact ccatttatta tgaccggaca tatcttaata
     3901 ctcccgtatt atttgagatc ttatcaatcc ccgcctcatc tctacttacg gcgggttgtg
     3961 gaatccggac cctggaattc catgcccgcc ggctttggac cggcatggta gaatgttttt
     4021 aggggtgttt attaattctt cttcttccat ctgaacaaaa gcgttatggt tcgtgcaaag
     4081 tcttactttt ataacacatg tcggcgtgca tgtaaaataa aaaacccctt cgcaagcgaa
     4141 ggggtttttt aaataatccc gg
//
LOCUS       DAOSMF010000156         4139 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_23381_length_4139_cov_19.806562, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000156 DAOSMF010000000
VERSION     DAOSMF010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4139
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23381_length_4139_cov_19.806562"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="PLT63_10240"
     CDS             complement(<1..253)
                     /locus_tag="PLT63_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99140.1"
                     /translation="MNRAPRAASFFCLPTVEKWYKYPYTPWDAAFEATIQATAGFLNV
                     SLPANLKWLAVKEGNYIESKGPVLANIAVDRFHVGNRRPA"
     gene            complement(350..664)
                     /locus_tag="PLT63_10245"
     CDS             complement(350..664)
                     /locus_tag="PLT63_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99141.1"
                     /translation="MKKIAMAFVVVLATSVLTTSAFAFGPAWRQDHRPNPQVRHQPAP
                     FPAQVQHSVYHHRPAVIVPVHPVRPVAYVPVRPVRPVVYAPEPARAGIVISIPNLSLV
                     IR"
     gene            complement(967..1776)
                     /locus_tag="PLT63_10250"
     CDS             complement(967..1776)
                     /locus_tag="PLT63_10250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOD99142.1"
                     /translation="MNTKYLKGRLYKMKIAELQPDPEQARKFMDPIALKELAASISQH
                     GVLTPIQFRQNAEGAPVIVSGHRRVRAAGMAGRTEIPATFTAGDTRLQGFVENIQREG
                     LLPIEEAEEMDRLMGAYNLNQTQLAEAIGKDQPHVSRALTLNRLPEDIRNACRTNPSF
                     SKATLLDFARMKTETAMRRKFDNYMKKAAAEGQTAASKPRLTPARALIKSTDELTGKL
                     DGLPWRDWSEDDRNDLVGALAGIRTKAGELLADMGYTPEEEEVPSASINLS"
     gene            complement(1878..2570)
                     /locus_tag="PLT63_10255"
     CDS             complement(1878..2570)
                     /locus_tag="PLT63_10255"
                     /inference="COORDINATES: protein motif:HMM:TIGR04283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HOD99143.1"
                     /translation="MSPRFSIIIPVLHEQAVITATIANLGELLYREDSDGEIIVVDGD
                     AEGKTIETLAGTGVKTTVGIKGRGNQMNRGAALADGDILLFLHADTRLPPGAFGLMET
                     ALQNTGCRAGAFDLAIASDRPVFRMIAGIASIRSRLTRIPYGDQGFFFHRDCFQELGG
                     FATIPIMEDVEIMRRLKSRGEEIAIIPHPVMTSARRWEREGIIRCTLRNWALIILYLC
                     GVQPERLARCYR"
     gene            complement(2570..3250)
                     /locus_tag="PLT63_10260"
     CDS             complement(2570..3250)
                     /locus_tag="PLT63_10260"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HOD99144.1"
                     /translation="MHPQRCLVMFAKYPAPGKVKTRLNPHFPEEMIVRLYRAFIEDLL
                     ARLAHGDYEFRFAYHPPDREEDFRRAFGDNYTYLPQTGGQLGEKMAGAFRACFAEGFR
                     EVVLIGSDSPDLPREIIGEAFGKLSDHGAVLGPADDGGYYLIGFRREAFTARVFTGIA
                     WGGKDVAAKTLAILAREETRVHVLPLWRDIDAPADLAALIAAGRKRDFTGSRTMACLH
                     DYGLIAPA"
     gene            3598..4101
                     /locus_tag="PLT63_10265"
     CDS             3598..4101
                     /locus_tag="PLT63_10265"
                     /inference="COORDINATES: protein motif:HMM:NF018942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HOD99145.1"
                     /translation="MVDHKAIISNKSLKIRGLFHRGLAHPRFYLLIRMALAALFIYGG
                     VVKLFDPKAFAATISAYDLVPEAILPMVAVGLPLIETIAGLALIFDRPWGLHLITGLL
                     ALFVFVLGYGVLGDLNVDCGCFGADDLNKQAGLRKAFYRDLVLIGIVMPYLYLSRWVR
                     EAEGRGR"
BASE COUNT          810 a         1150 c         1252 g          927 t
ORIGIN      
        1 cggcggggcg gcgattgccg acgtgaaaac gatcaacggc aatattagcc aggacaggac
       61 ccttgctttc aatataattg ccttctttca ccgccagcca tttcaagttg gcaggcagcg
      121 aaacattcaa aaatcccgcg gttgcctgaa ttgtcgcctc aaatgcggca tcccaaggag
      181 tatatggata tttataccat ttttccaccg taggcaaaca aaaaaaagaa gccgccctgg
      241 gggcccggtt catgagcccc cctgctctac attatggata tgatgctccg catttccgca
      301 ttttcgggcg ttggtcaaaa acatccgttc gggccggaga agtcgtttgc taacgaatca
      361 ccaggctcaa attcgggatg gagatgacta ttcccgcccg cgccggctcc ggggcgtaaa
      421 ccaccggccg cacagggcgc accggcacat acgccaccgg ccgtacggga tggaccggaa
      481 caatcaccgc cggccggtga tgatagacag aatgttggac ctgagcgggg aaaggggccg
      541 gctgatgcct cacctgggga ttgggacgat ggtcttgccg ccaggctggg ccgaaggcaa
      601 aggcggaagt tgtcaaaacg gacgtggcga gcacgacgac aaatgccatg gcgatctttt
      661 tcattgttct gttctccttt caatggtctt tatttgtgtt tgatctggat ggagcataaa
      721 acgtaccaga cgggaaatag acgcccatgt ccttgaaaat aaaggatatt atccggagat
      781 cgagaactcc cggtgtttcc ggcggccgtt gctcgccttg aggatggata aaaatattac
      841 attttcagcc gcgtcatgga taaaatatat ccgcattgcc ggacctgatg gtgatcatcg
      901 cgttccggtt tttttcgctt acatattata aatatcaata tgttgcaatt gagcggctcc
      961 ggggcgtcag gaaagattta tcgaggcgga cggcacctct tcctcctccg gcgtgtagcc
     1021 catgtcggcc agaagctcgc cggctttggt gcggatcccg gccagggcgc cgaccagatc
     1081 gttccggtcg tcttccgacc agtcccgcca ggggagcccg tccagcttcc cggtcagttc
     1141 atcggtgctc ttgatgaggg cacgcgccgg ggtaagcctc ggttttgacg ccgcggtttg
     1201 accttccgcg gcggccttct tcatatagtt atcgaacttc cggcgcatcg ccgtttccgt
     1261 cttcatgcgg gcgaagtcga ggagtgtcgc tttggagaaa ctgggattgg tccggcaggc
     1321 attgcgaata tcctccggga gacggttaag ggtaagggct cgggagacat ggggctgatc
     1381 cttgccgatg gcctcggcaa gctgggtctg attgaggttg tatgccccca tcagccggtc
     1441 catctcttcc gcttcctcga tgggcaggag tccttcccgc tggatgtttt ccacgaagcc
     1501 ctggaggcgg gtgtcgccgg cggtgaaggt ggcgggtatt tccgtccgcc ccgccatgcc
     1561 cgccgcccgg acgcggcgat ggccggagac gatgaccggc gccccctcgg cgttctggcg
     1621 gaactggatg ggcgtcaaga ccccgtgttg acttatcgaa gcggccagtt ccttgagggc
     1681 gatgggatcc atgaatttac gggcctgttc cgggtccggc tggagttcgg cgatcttcat
     1741 cttgtaaagg cgtcccttga gatatttggt gttcatgggc aaatcctccc gtggtatttt
     1801 acctacataa tatgtatttt catcggaaag tcaaggagat ggacagggat atcaagggca
     1861 ggcagagagg tcgaggatca tcgatagcat ctggccagcc gctccggctg gacgccgcag
     1921 aggtaaagga tgatcagcgc ccagttgcgc agggtgcacc ggatgattcc ctcccgctcc
     1981 cagcggcgcg ccgaggtcat aaccggatgt gggatgatgg cgatctcttc ccccctgctt
     2041 ttgaggcgcc gcatgatctc cacatcctcc atgatgggga tggtcgcgaa tccccccaat
     2101 tcctgaaagc aatcccggtg gaagaagaag ccctgatcgc cgtaggggat acgggtgagt
     2161 cgggaacgga tcgaggcgat gccggcgatc attctgaata ccggccggtc ggaggcgatg
     2221 gccagatcga aggcgccggc gcggcaaccg gtattctgca acgctgtttc catgaggcca
     2281 aaggccccgg ggggaaggcg ggtgtcggcg tgcaaaaaga ggaggatgtc gccgtccgcc
     2341 aacgcggcgc ccctgttcat ctggtttccc cgacccttga tgccgacggt ggtttttact
     2401 ccggtgccgg cgagagtctc aatggttttg ccctctgcgt ctccgtccac aacgataatc
     2461 tccccatctg aatcttccct ataaaggagc tcccccaagt tggcgatggt ggctgtgatg
     2521 acggcctgct catggaggac ggggatgatt atggaaaaac ggggcgacat caggcgggag
     2581 cgatgaggcc gtaatcatgg agacaggcca tggttcgtga accggtgaag tccctcttcc
     2641 tcccggccgc gataagggcg gccaaatccg cgggtgcgtc gatatccctc cagaggggca
     2701 ggacatggac ccttgtttcc tcccgggcca ggatcgccag ggttttggcg gcgacatctt
     2761 tcccgcccca ggcgatgccg gtgaagaccc gggccgtgaa ggcttcccgg cgaaaaccga
     2821 tgaggtaata tcccccgtcg tcggctggtc ccaagacggc gccgtgatcc gacaatttcc
     2881 caaaggcctc cccgatgatc tcccggggga gatcagggct gtcgctgccg atcaggacca
     2941 cctcccggaa gccctcggca aagcatgccc gaaaggcgcc ggccatcttt tccccgagct
     3001 ggccgcccgt ctgggggagg taagtgtagt tatctccaaa ggcccggcgg aaatcctctt
     3061 cccggtccgg ggggtggtaa gcgaaacgga actcgtagtc gccgtgggca agtcttgcca
     3121 aaagatcctc gatgaacgcc cgatagagac ggacgatcat ttcctcaggg aagtgggggt
     3181 tgagacgggt tttaaccttt cccggggccg ggtatttggc gaacatgaca aggcatcgct
     3241 gggggtgcat ttgctcggcg ccctcctttc aggcggcaag catagaaaag aatgtccggg
     3301 aaagtcaagg tggtgtttcc ttgaccggca gcatgcttcg cgtgttcaga ttgttgttga
     3361 cacagcctgc ccgattggtt atatacgcgc cgttacctta ttctttttta aaaggaatca
     3421 ggagtaaaat cgatgggtta tttgtacttg tacttcatac cgttaatatg aggcgttgac
     3481 cgaggacccg ggtggttctc ccgacaacgc cgtcctgccg tttctccacc gccgaccgtt
     3541 tagcctgctg atggtcatgc cggggggagc cggtcatgac gacatcagga gttgaaattg
     3601 gtcgatcata aagcgataat ttcaaacaag tcattgaaaa tcagggggtt gttccatcgc
     3661 ggcctggccc atccccgttt ctatctgctg atccgcatgg ccctggccgc cctgttcatc
     3721 tatggcggcg tcgtcaaact cttcgatccc aaggcatttg ccgccaccat ctccgcttac
     3781 gatctcgtcc cggaggctat ccttcccatg gtcgccgtcg gtctgcccct catcgaaacg
     3841 attgccggtc tggccctgat ctttgaccgt ccctggggac tccatctgat cacggggctt
     3901 ttggcgctct tcgtctttgt cctcggttac ggcgtccttg gcgatctgaa cgtcgattgc
     3961 ggctgcttcg gggcggatga tctcaataag caggccggtc tgcggaaggc gttttaccgc
     4021 gacctggtcc tcatcgggat cgtcatgccc tacctttacc tttcgcggtg ggtgcgggag
     4081 gcggagggcc gcggccgtta ggatcggtag gtaggtggaa accggatttg acgagtgtg
//
LOCUS       DAOSMF010000157         4136 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_23404_length_4136_cov_24.919628, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000157 DAOSMF010000000
VERSION     DAOSMF010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23404_length_4136_cov_24.919628"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..911)
                     /locus_tag="PLT63_10270"
     CDS             complement(120..911)
                     /locus_tag="PLT63_10270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOD99146.1"
                     /translation="MTYERGKLYEVPVIDFKNDPNQPRKYMDPQGLEELAASIRTHGI
                     LEPVLFRSGDQGWVIIVAGERRLAAAKMAGLHVIPGICVEGNHAEIALVENLLRQDLT
                     AVEEAEALKRLMDEQNYTQEQLAGVIGKPRTTINEMLLINRLPPEIRDECRGNTDIPK
                     SDLVEISRKKQERGMRSAFEKLKERLQKEREGKRPREVKTPAGVLCALLEKTRERLDK
                     AEIADWSDADLAALNDAVAGLREALDAFENPSEDPGAAGPIRELS"
     gene            complement(1334..1588)
                     /locus_tag="PLT63_10275"
     CDS             complement(1334..1588)
                     /locus_tag="PLT63_10275"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOD99147.1"
                     /translation="MTAYAGVRHAVSCASGTDALLMAPMAYGIGPGNAVFTTPFTFVA
                     TAEVISLLGATPVLETFEKWLRHGSEIFFASEWVTYESYS"
     gene            complement(1850..2479)
                     /locus_tag="PLT63_10280"
     CDS             complement(1850..2479)
                     /locus_tag="PLT63_10280"
                     /inference="COORDINATES: protein motif:HMM:NF025468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4276 family protein"
                     /protein_id="HOD99148.1"
                     /translation="MVGEIRIYVEGGGDQRSGKAALREGFSKFLSSVREKARTQRVRW
                     NIIACGSTRNAVEDYQNALERHRDAANILLVDSDGPLLLPPLDYLKRNGGFDVEGMSP
                     KLVHLMVQVMETWLLSDRGALTRFYGAGFNRNALPRRSNMEEVDKRDVYQALSDATLN
                     TQKGEYHKIRHGPKILAMLDADRVRRRAAYCDRFFRTLEELIEENGTLP"
     gene            complement(2487..3710)
                     /locus_tag="PLT63_10285"
     CDS             complement(2487..3710)
                     /locus_tag="PLT63_10285"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOD99149.1"
                     /translation="MSGSKKLIRSLRLRNFLSFGPDSEEINLESLNVLIGPNAAGKSN
                     LIDAVDFLRSSCKDFSGPIRQGGGVEEWLWKGAAKTPVAEVEATVFYPNGIMPLRHRI
                     SFTMTGQRFELADEAVENERPDVADKSDVRFYYRYQKGHPVISAYPESSESENGSENR
                     TQRKLRREDIALDQSILSQRKEPDMYPEITYLATQYARIMIYRDWTIGRFTSARMPQE
                     TKLPEDILLENSANLGLVINHLEHRSGLRKLFLDKMKEFHEPFEDVSVKIHAGTVQVF
                     LHERGLITPVPATRLSDGTLRYLSLLAILCHPEPPPLVCIEDPELGLHPDILPSLAAL
                     MIEASQRMQLIVTTQSDILVDSLTDTPEAVLVCDKEDGGTTVKRLDRKDLGNWLKKYA
                     LGEIWRMGEIGGKRW"
BASE COUNT          873 a         1221 c         1080 g          962 t
ORIGIN      
        1 caaagctcac cgggcacatt tacggggatg acgaaatttc gtcactatat aacttactga
       61 tattattgaa taattaaata ttaatcaaaa aatttgccca gtcactaact gaccatcggt
      121 caggatagtt cccggatggg tcccgctgcg ccggggtcct cggaagggtt ttcaaaggca
      181 tccagggcct cccggaggcc ggcgacggca tcgttgagcg ccgccagatc cgcgtcggac
      241 cagtcggcga tctccgcctt gtccagccgc tccctcgttt tctccaggag ggcgcagaga
      301 acgcccgccg gcgtcttcac ttcccggggt cttttcccct cccgttcctt ctggaggcgc
      361 tccttgagtt tttcaaaggc cgaccgcatg ccccgctcct gcttcttccg ggatatctcc
      421 acgaggtccg atttcgggat gtcggtgttg ccccggcatt cgtcgcggat ctccgggggc
      481 aggcgattga tgaggagcat ctcgttgatc gtcgtccggg gcttgccgat gaccccggcc
      541 agttgctcct gggtgtagtt ttgttcatcc atgaggcgtt tcaaggcctc cgcctcctcc
      601 acggcggtga ggtcctggcg gaggaggttc tccaccaggg cgatctcggc gtggttcccc
      661 tccacgcaga tcccggggat gacgtggagg ccggccatct tcgccgctgc caggcgcctc
      721 tcccccgcca cgatgatgac ccagccctga tctcccgatc ggaagaggac cggctccagg
      781 atgccgtggg tccggatgga ggcggccagt tcctccaggc cctggggatc catgtatttg
      841 cggggctgat tcggatcgtt cttgaaatcg ataaccggaa cctcataaag cttaccgcgt
      901 tcatatgtca tggcgcttat ccctcctttc gcttgtcgtt gccggtcccg gtccgcgccc
      961 cgtcacgtcg ccctccccgg cggcggcatc agcggtcgcc ggggagggca cccgctttca
     1021 cttcgaccgg caaacaggtc ctggataacg tcggcatcgt cgccacggcg ggggccgggg
     1081 cgatggcccc gccggtgccg ataaccgcta tatatttccg ttgcccccta agtcaatcct
     1141 ttccataggc atgtctcctc aggcaaccgc cgcccgccgc aggatcgccg ccgcccgctc
     1201 ctgggcggga tcgaggttgt agatttccgg ctcgatatcc acaagcagag agttttgcat
     1261 aactgcggaa aaatggttcg caagcggcga aacaaatgtt tttcgccatt tcgtgattga
     1321 acttaacgcc tggttatgaa taagattcat acgttaccca ttcagaagcg aaaaagattt
     1381 ctgagccgtg gcgaagccat ttttcaaagg tctcaagcac cggcgtcgcc cccagcaggc
     1441 tgatgacttc cgccgtggcc acgaaggtga acggggtggt gaagacggcg ttgccgggac
     1501 cgatgccgta agccatcggg gccatgagca gggcatccgt gccggaggcg caactcacgg
     1561 cgtgccggac gccggcataa gccgtcagtc cgtcgaggaa ggttcatcct gcagggaagt
     1621 catctggatg gctttggtca tgggttacct ttataaaaac ggacgcactc ttcatccatt
     1681 catgacgaca tcgtaataag tcaaaaaatg tcccatttgg gacggcatcg taaaaagatc
     1741 cgcaggcgag gcgcgcgaat cctgaggaat gaggcgtact tggacgtacg tcgcagtgac
     1801 gagggatgaa gcgctttcga agcatccgga ctttttacga tgccgtcatt catggaaggg
     1861 tgccgttttc ttcgatcaac tcttctaatg tacggaagaa ccggtcacaa tatgctgccc
     1921 gcctccgcac cctatcggca tcgagcatcg ccagaatctt gggcccatgg cgaatcttgt
     1981 gatattcacc tttttgtgtg tttagcgtcg cgtcgctcag cgcctgatac acatcccttt
     2041 tatcgacctc ttccatgttt gaccggcgcg gcaaggcatt cctgttaaat cctgcaccat
     2101 aaaaccgcgt caaggcgccc cggtctgaaa ggagccaggt ttccatcacc tgtaccatca
     2161 gatgcacaag ctttggggac atgccttcca catcgaagcc cccattcctc tttaagtagt
     2221 ccaaaggcgg caacaacaac ggcccgtcgg aatcgaccaa gaggatatta gcggcgtcac
     2281 gatgcctttc gagagcgttc tggtagtctt ccacggcatt tcgggtagag ccacaggcga
     2341 taatattcca gcgcacacgc tgcgtcctgg ccttctctcg cacactggac aaaaacttgc
     2401 tgaaaccctc tcggagagcg gccttgccgc tacgctgatc cccgccaccc tctacgtaaa
     2461 tcctgatctc gcctaccata tctcactcac caacgttttc ccccgatctc ccccatgcgc
     2521 caaatctctc ccaaggcgta cttcttgagc cagttcccaa ggtccttgcg atctaagcgc
     2581 ttcacggtgg tgccaccatc ctccttgtcg caaaccagaa ccgcttcagg cgtatccgtc
     2641 agactgtcca caaggatgtc ggactgggtc gtaacgatca gttgcatgcg ctgggatgct
     2701 tcgatcatca gtgccgcaag ggacgggaga atgtccggat gtaatcccaa ttccgggtcc
     2761 tcgatgcaaa ccagcggcgg gggctccgga tggcacaata tggctagtaa agacaggtac
     2821 cgaagcgtgc cgtccgacag acgggtggcc ggcaccggcg tgatcaatcc tctttcatga
     2881 aggaaaacct gcacggtccc cgcatgaatc ttaacggaaa catcctcgaa cggctcgtga
     2941 aattccttca tcttatccag gaaaagcttt ctcaggccag atcgatgctc cagatggttg
     3001 atgaccagcc caagattggc ggaattttcc aaaaggatat cctcaggcag cttcgtttct
     3061 tgaggcatgc gggccgaggt aaagcgtccg atggtccagt ctcgatagat catgattcta
     3121 gcgtattggg tggccagata ggtaatctcg ggatacatat ccggttcctt gcgctgagac
     3181 aaaatggatt ggtccagggc gatatcttcc cggcgaagtt ttcgctgggt gcggttttca
     3241 gatccgtttt ccgattccga agactcgggg taggcgctga tcacgggatg ccccttctga
     3301 tacctgtaat aaaaacgcac atccgactta tcggcaacat cgggccgctc gttctccacg
     3361 gcttcgtcag ccagttcgaa acgctgcccc gtcattgtga aggaaattct gtgccgcaat
     3421 ggcataatcc cgttgggata aaatacggtc gcttcaacct ccgccacggg tgttttcgcc
     3481 gcgcctttcc aaagccactc ctcgacgccg cccccctgac gaatcggacc cgagaaatct
     3541 ttacaggagc tccgcaaaaa gtccaccgcg tcaatcaaat tggatttccc cgcggcgttc
     3601 ggcccgatca aaacattcaa ggattccagg tttatctcct cagaatcagg gccaaaggaa
     3661 agaaaattcc gcaaccgaag agaacgaatc agttttttac ttcccgacat gatctattca
     3721 cctcgctttg ccgcgccgtt gtttgcgcgt ctttgcctgc cgtatttggc aaactataat
     3781 tccgactcca ggttctcatt gcgcgtatta gcagtagcgt tcgggtgcca cagcaggtgc
     3841 aggatgccgc aggctccgaa aaaacgcaag ctatttcccg catgtcgctt tcttgttggg
     3901 cgatgcgagt ctagtaggag tcaggctcat ctaactcccc ccttatgcat gttatgttct
     3961 ctgcaaaggg gatagatatc ccccttctga tcagtgcttc cgccaccgtt aaaccgagat
     4021 cctgcattca tcttgttgta cccattttct tactcgatag tatttttgcc taaaccattc
     4081 ttgatgacat cgtaaaaagc gcaaaaaatg tcccatttgg gacggcatcg taaaaa
//
LOCUS       DAOSMF010000158         4085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_23772_length_4085_cov_23.853846, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000158 DAOSMF010000000
VERSION     DAOSMF010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4085
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23772_length_4085_cov_23.853846"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1335
                     /locus_tag="PLT63_10290"
     CDS             <1..1335
                     /locus_tag="PLT63_10290"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOD99150.1"
                     /translation="LSDEKKLGKEFYDNLEKHQAILHDQRAATYLSTIGNMVLANSEK
                     LPYDYRFSIIKSSAVNAFATPGGYIYVNHGLLTLVESESELASVLAHEIAHINARHIA
                     DSIEKSKKLNIAALAGILAGAFLGVGSDLGAAAVAFPMAAMQTMSLKYSREHEEEADR
                     LGLSYLVSAGYDGKSALDFLKILRRYEFYSNSIPSYFLTHPGTEDRIRYLDAMLQTTY
                     TRRGKESIVGNLKKIQTLLILQTKDYQKNLKHFQENLQKNPRNADDLFGLAVTQERLG
                     LTAESIQTFHEAHSLAPSDPDILREMGIAYFKAGKSKEAVPYLQEAIRRDPGNVSALL
                     YLARAYEAQGRKTEAIAIFKEIEQKTVDDEEVFYTMAMIYGQNNHLYESHYYFGRYFK
                     KKNRRESALYHFQAALKHVPADGSEAYEIQKEIQDLKSPGGNKPEKAPTKSY"
     gene            complement(1329..2870)
                     /locus_tag="PLT63_10295"
     CDS             complement(1329..2870)
                     /locus_tag="PLT63_10295"
                     /inference="COORDINATES: protein motif:HMM:NF025499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HOD99151.1"
                     /translation="MKFSPADIEQIQREGLSVARVEEQLALFQRGVSHIELHRPCRTG
                     DGIEVISPEAGERLTSRCELAAAAGRCLKFVPASGAASRMFKDWFQGLEDPWREGDPA
                     AAAFAANLPRYPFYEDLRLVMARQGDDPDTCLREKRYDALLSSILTPHGLGYGYLPKA
                     LLKFHHYPDGSRTALEEHLVEAALYATDNRRISRIHITVSPEYRLAVTDFLRETCPRY
                     EGIFSVTYDIAVSCQETATNTIAVDMDNRPFRTEDERLYFRPGGHGTLLKNLNDIDGD
                     IIILKNIDNVAPDHLKEVTVRYKKILAGLLLERQEESFRYLQCLRAEHVDAETIAAAD
                     RFCREKLHITFPDDYHRWSPEKKKAVQLRKLARPMRVCGVVRNEGEPGGGPFWVRDDD
                     GGRSLQIVEEIQVDPHSESQQAIWRAASHFNPVDLVCGVRDYRGEKYDLRRFVNPRTV
                     SIAKKSEKGRDLQALELPGLWNGSMADWNTIFVEVPIATFNPVKTIADLLRPQHLWQR
                     NNGGQ"
     gene            complement(2867..3646)
                     /locus_tag="PLT63_10300"
     CDS             complement(2867..3646)
                     /locus_tag="PLT63_10300"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOD99152.1"
                     /translation="MDTSLEAGKPELRVIIDRDKAADLGVTVASIAAKILPMDYTAKY
                     KGAADTMKESPGLLLFALLRGTVMAYMVLAAQFESFMQPVIVLLSMPLSFIGAFGALV
                     LTGKTISIFSMIGMILLMGLVKKNAILLVDYTNTLRERGMPRREAILEAGPVRLRPIL
                     MTTFAMIFGMLPVAAGLGEGAETRSPMGVAVIGGLITSLFLTLIVVPAAYDIMVIGLQ
                     FMAKQTVTRRQKDISRLLRERRRKWKDADGKMKNRKGIKKK"
     gene            complement(3862..>4085)
                     /locus_tag="PLT63_10305"
     CDS             complement(3862..>4085)
                     /locus_tag="PLT63_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99153.1"
                     /translation="GGLLYMTICAGFIALIIVLEAGPVYAVFMAGIRGQVLTAPQWFW
                     LIGSLGLAFAVCAFAAAAPLRMGVKRLEC"
BASE COUNT          946 a         1218 c         1079 g          842 t
ORIGIN      
        1 ctgagcgatg aaaaaaaact gggcaaggaa ttctacgaca acctcgaaaa gcaccaggcc
       61 atcctacatg accagcgggc cgccacctat ctgtccacca tcgggaacat ggtcctggcg
      121 aacagtgaaa agcttcccta cgattaccgt ttttccatca tcaagagttc ggcggtcaac
      181 gccttcgcca ccccgggggg ctatatttat gtcaaccacg gccttttgac cctcgtcgaa
      241 tcggaaagcg agctggccag cgttctggcc catgaaattg cccacatcaa cgcccggcac
      301 atagcggata gcatcgaaaa atcgaaaaag ttgaatattg cggccctggc ggggatcctt
      361 gccggcgcct tcctcggtgt tggcagcgat ctgggcgccg cggccgtggc attccccatg
      421 gccgccatgc agaccatgag cctgaaatac agccgcgagc acgaagaaga ggccgatcgg
      481 ctcgggctca gctatctcgt gtcggccggc tacgacggaa aatctgctct tgatttttta
      541 aagatcctcc ggcgttacga gttctattcc aattccattc cgtcgtactt tctcacccat
      601 cccggtacgg aagatcggat ccgctatctc gacgccatgc tgcagactac ctatacccgg
      661 cggggcaaag aatcgatcgt cggcaaccta aaaaaaattc agacgctcct gatcctccaa
      721 accaaggact atcagaagaa tctcaaacat tttcaggaaa atctccagaa gaacccccgc
      781 aacgcggacg atcttttcgg cctcgcggtg acccaggaga ggctcgggct gaccgccgag
      841 tccatccaga cctttcatga ggcacattcc ctcgccccgt cggatcccga tatcctcaga
      901 gaaatgggga ttgcttattt caaggcgggg aaatcaaaag aggcggttcc ctatctccag
      961 gaggcgatcc gtcgcgatcc cggaaatgtt tcggccctgc tttaccttgc ccgggcgtac
     1021 gaagctcagg gcaggaagac cgaggccatc gccatcttca aggaaatcga gcaaaagacc
     1081 gtggacgacg aggaggtttt ttatacgatg gcgatgatct acgggcagaa caaccatctg
     1141 tatgaatcac attactactt tggccgatac ttcaagaaaa agaaccgccg ggaaagcgcc
     1201 ctgtatcact ttcaggccgc cttgaaacac gtaccggccg acggttcgga agcctacgag
     1261 atccaaaagg aaattcagga cctcaagagt cccggcggga ataaaccgga aaaagctccg
     1321 acaaaatcct attgaccacc gttgtttcgt tgccagaggt gctgtggccg cagcaggtcg
     1381 gcaatcgtct tgaccggatt gaacgtggcg attggaacct ccacaaaaat ggtgttccag
     1441 tccgccatgg agccgttcca tagtccgggc agttccaggg cctgcaaatc ccgccctttt
     1501 tcggattttt tggcaatgga gacggtcctc ggattgacga aacgcctcag gtcgtatttt
     1561 tctccccgat aatcccgaac cccgcagacc agatcaacgg gattgaaatg ggaagcggcc
     1621 cgccagatcg cctgttggga ttccgaatgg ggatcgacct ggatctcctc cacgatttgc
     1681 agcgaccggc cgccgtcgtc gtctcggacc cagaaggggc ctccccccgg ttcgccttcg
     1741 ttgcgaacga caccgcacac ccgcataggc cgcgccagtt tccgaagctg gacggccttc
     1801 tttttttccg gcgaccagcg gtgataatca tcgggaaaag tgatatggag cttctcccgg
     1861 caaaatcgat ccgctgccgc aatggtttcc gcatcgacat gctccgcccg cagacattgc
     1921 agatatcgga agctctcttc ctggcgctcc aaaagcaatc ccgccagaat tttcttgtag
     1981 cgcaccgtca cctccttgag gtgatccggt gcaacattgt cgatattctt gaggataatg
     2041 atgtcaccgt cgatgtcgtt caaattcttg agcagggtgc cgtgcccccc cggccggaaa
     2101 tagagacgtt cgtcctccgt ccgaaagggc cggttgtcca tatccacggc aatggtgttg
     2161 gtcgccgtct cctggcagga cacggcgatg tcataagtga cgctgaagat cccttcatag
     2221 cgcgggcagg tctcccggag aaagtccgtc acggccaagc ggtattccgg cgatacggtg
     2281 atatggattc tgctgatccg ccggttgtcc gtggcgtaga gggcggcctc caccagatgc
     2341 tcttccaggg ccgtccggct tccgtccgga taatggtgaa atttgagcaa ggccttgggg
     2401 agatagccat aacctagccc atggggggtc agaatagatg acaggagcgc atcataccgc
     2461 ttctccctca aacaggtatc gggatcgtcc ccctgccggg ccataaccag gcgcaggtcc
     2521 tcgtaaaagg gatagcgggg gagattcgcg gcgaaagccg ccgccgccgg atcaccctcc
     2581 cgccacggat cttccagccc ctgaaaccaa tccttgaaca tgcggctggc ggccccggag
     2641 gcaggaacga atttcaggca acggccggcg gccgcggcca gttcgcaacg ggacgtcagc
     2701 ctttcccctg cttccggaga aatgacctcg atgccgtcgc cggtccggca gggcctatgc
     2761 agctcgatat gagaaacacc tcgctgaaat agggcgagct gctcctccac ccgtgccacg
     2821 gagagaccct cgcgttgaat ctgttcgata tcggcggggg aaaatttcat ttcttcttaa
     2881 tccctttccg gtttttcatc ttcccgtcgg catctttcca tttccgtcgc cgctcacgaa
     2941 ggagccggga gatgtccttc tgccgcctgg ttacggtttg tttagccata aactgaagtc
     3001 caattaccat gatatcatag gccgccggca cgacgatcaa ggtcagaaaa agcgaggtta
     3061 taagcccgcc gattaccgct acccccatgg gggatcgggt ctccgcgccc tcccccaatc
     3121 ccgcggccac cggcagcata ccaaaaatca tggcaaaagt cgtcataaga atgggccgca
     3181 accggaccgg acctgcctcc agaatggcct cccggcgcgg cattccccgc tcccggagcg
     3241 tattggtgta atccaccaga aggatggcat tctttttcac cagtcccatg aggagaatca
     3301 tcccgatcat actgaagatg ctgatggttt tccccgtcag gaccagggca ccgaaggccc
     3361 cgatgaaaga caggggcatg gagagcaata caatcaccgg ttgcatgaag ctctcgaatt
     3421 gcgccgccag aaccatatag gccatgaccg tgccccgcag cagggcaaag agcagcaaac
     3481 cgggcgattc tttcatggta tcggcagcgc ccttgtactt cgccgtataa tccataggaa
     3541 ggatttttgc ggcgatgctg gccacggtta cacctagatc cgctgccttg tcccggtcga
     3601 tgatcacccg caattccggt tttcccgctt ccagcgatgt atccacatcg acgatgccgg
     3661 gaagcttagc ccaagttcat ggtttttcct caaaaaatgg cttttgggtg tcattattca
     3721 agagtcactt ctaataatat ctgatactta taagttatgg cccgacaaaa agagtgaact
     3781 accaccgagc aagctcggtg gtagttcact caatgattgt tacgttacaa gcagaaaact
     3841 ttggactctc aagatattaa gtcagcattc caggcgcttt actcccatcc gcaggggcgc
     3901 cgccgcggca aaggcgcaga cggcaaacgc cagtcccaat gagccgatca gccagaacca
     3961 ttgcggggcg gtcaggacct gccccctgat ccccgccatg aagacggcat aaaccggtcc
     4021 cgcctccagg acgatgatca gggcgatgaa gccggcacag atcgtcatgt agaggagtcc
     4081 gccga
//
LOCUS       DAOSMF010000159         4027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_24199_length_4027_cov_22.202165, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000159 DAOSMF010000000
VERSION     DAOSMF010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24199_length_4027_cov_22.202165"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..1567
                     /gene="holA"
                     /locus_tag="PLT63_10310"
     CDS             158..1567
                     /gene="holA"
                     /locus_tag="PLT63_10310"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOD99154.1"
                     /translation="MNTDNKGPHADGDRILREIQGGRIAPAYLLWGEEFLVREALDGI
                     VNALVPEADRAFNLFFITPEQADVDRLCAELSAASLLPGTKVVVVRQAPFLLSGRQSP
                     AQIVEKIRECLEGEPRRAAREFITLLGMAGWSLADLQEDGWRRIDAAEWTRLLPGGGS
                     DELEKWLPRVLEICAGQEGLSPGRKTGTDRLEELLMSGLPAGNVLILTAPNIDKRKRL
                     VKILTEKGRVVQFSAGRGEARQQSLVMAKTRALLEGYGKALTPEAWQALGRRTGFDLS
                     ASTQALEKLAVFTGERREIRPEDVEEIIGKTKEESVFDLTAALGERAAARALRILREL
                     LVDQGVQPLVVLAMLVREVRFLLHARIFIHGGRLPSWRSGMDYGQFQARIMPEIKALA
                     AGREEKGEAGDLADENPYVIFQACRRARDFSEDTLIACLRELARLDVQMKTTGRNPHL
                     LLENFILNFCADRNAGVRV"
     gene            complement(1557..>1798)
                     /locus_tag="PLT63_10315"
     CDS             complement(1557..>1798)
                     /locus_tag="PLT63_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99155.1"
                     /translation="SDRDIGNFVERCGGTVVASPTFAAIVTGDHGPSAGHKPESGLDG
                     RDEEPAAGKRGTKKKGPARKISRREREYLKRLVKL"
     assembly_gap    1799..1898
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1899..2248)
                     /locus_tag="PLT63_10320"
     CDS             complement(<1899..2248)
                     /locus_tag="PLT63_10320"
                     /inference="COORDINATES: protein motif:HMM:NF017774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOD99156.1"
                     /translation="MHIIIDGYNLIRQSEQLRRAERLTLEEGRQALLRLLIPYRERKG
                     HRLTVVFDGWEGGSAHEERDRAGGIDILYSRRGEKADDVIKRLAERSSYELVVVTSDR
                     DIGNFVERCGGTVVA"
     gene            2528..>4027
                     /locus_tag="PLT63_10325"
     CDS             2528..>4027
                     /locus_tag="PLT63_10325"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HOD99157.1"
                     /translation="MAEKVYAPGEPYKYQLLIKHILEMPLAFAPNQEIVYRDKVRLTY
                     RTLNERIQRLANGLEKIGAGPGDRICIFDYDSHRYLECFFAVPMMGAIMHTQNWRLSP
                     EQILYTMNHAEDKIVLIHQDFVPLLEAIWDKLATVKKVILITDDGNRPTSKVPFDAEY
                     EEMLASSSPAHDFPDLDENTQATIFYTTGTTGLPKGVFFSHRQLVLHAQALLLATGSY
                     ASIGRCQSNDVYMPITPMFHVHAWGFPWAATMLGVKQVYPGRYEPAMLLKLILTERVT
                     FSHCVPTIIQMLVSSPAAKQYDLSFWKVIIGGARLTKSLALEAMKMGIQIFAAYGMSE
                     TCPLLTAANLKPWMLKNSMEENVDTLVKTGLPAPLVYVRLKDPLGKDVPHDGVSTGEV
                     CVRSPWLTERYFKDPERSKELWTDGWLHTGDVGLIDAEGYLQVTDRIKDVIKTGGEWI
                     SSLELENIMSSHEAVLEAAAIGLPDAKWGERPLLVVVLKPDYAGKVTGEE"
BASE COUNT          774 a         1160 c         1203 g          790 t
ORIGIN      
        1 ggtaacgcag gcgtaaaata agcgactgat attattaccg cttcggttaa tgatggtgac
       61 tggttaatgg taaggggcga gtcgatacct tcccgcattg gttggccaag gatcggcggg
      121 tccggtaaga gatcgagcgg atcgacaggg agcgttgatg aacacggaca ataagggtcc
      181 ccacgccgac ggtgacagga ttctccggga gattcagggg ggaaggatcg ccccggccta
      241 tctcctctgg ggggaggagt ttctcgtccg cgaggccctg gacgggatcg tcaacgccct
      301 ggttcccgag gcggatcggg cgttcaatct cttcttcata acgccggagc aggcggatgt
      361 ggaccggctc tgcgcggagc tcagcgccgc gtccctcctc cccggaacca aggtggtcgt
      421 ggtccggcag gcgccgtttc tcctttccgg ccgccaatcg ccggcgcaga tagtcgaaaa
      481 aatccgggaa tgcctggaag gggagcctcg acgggcggcc agggaattca ttaccttgct
      541 ggggatggcg ggctggagcc tggccgatct ccaggaggac ggctggcggc ggatcgatgc
      601 ggcggaatgg acccgcctcc tgccgggagg cgggagcgat gaactggaaa aatggctgcc
      661 ccgggtcctg gagatctgtg ccggccagga gggcctctcc ccggggcgga aaacgggcac
      721 ggaccgtttg gaggaactcc ttatgtcggg gctgccggcg gggaacgtcc tgatccttac
      781 cgccccgaac atcgacaaac gcaagcgcct ggtaaagatc ctcaccgaaa agggccgggt
      841 ggttcagttt tccgccggcc ggggcgaagc ccgccaacag agcctggtca tggcgaaaac
      901 gagggcgctg ctggagggat atgggaaggc cctcactccg gaggcttggc aggcactggg
      961 ccggaggacg ggtttcgacc tgtccgcttc cacccaggcc ctggagaaac tggcggtttt
     1021 caccggggag cggcgggaga tccggccgga agatgtggag gagatcatcg gcaagacgaa
     1081 ggaggaaagc gtcttcgatc tgaccgccgc cctgggggaa agggccgccg cccgggccct
     1141 gcggattctc cgggaactcc ttgttgacca gggcgtgcag cccctggtcg tgctggctat
     1201 gctggtccgg gaggtccgtt ttctcctcca tgcccggatc ttcatccacg gtggccgcct
     1261 gccgtcctgg cggtcgggga tggattatgg gcagtttcag gccagaatta tgccggagat
     1321 caaggcactg gctgccggcc gggaggaaaa gggcgaggcg ggcgatctgg cggacgaaaa
     1381 tccctatgtc atcttccagg cctgccggcg ggcccgggac ttctccgaag acacattgat
     1441 cgcctgtctg cgggagctgg cccgactgga tgtgcagatg aaaaccaccg gccggaatcc
     1501 ccatctcctg ctcgaaaact tcatcctgaa tttctgtgcc gacaggaacg ccggcgtcag
     1561 agtttgacaa ggcgttttag gtactcccgc tcccggcggg atatttttct tgccggccct
     1621 ttcttcttcg ttcctctttt accggccgcc ggctcttcgt cccggccgtc gaggccgctt
     1681 tccggcttgt gccccgcaga aggcccatga tcgccggtga cgatggcggc gaaagtgggt
     1741 gatgccacca ccgtgccgcc gcaccgttcc acaaaattgc cgatgtcccg gtccgaggnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc caccaccgtg ccgccgcacc
     1921 gttccacaaa attgccgatg tcccggtccg aggtgaccac cacgagctca tagctcgatc
     1981 tctccgccag gcgcttgatg acgtcgtcgg ccttctcccc ccggcgggaa tagaggatat
     2041 cgatgccccc ggcccgatcc ctttcttcgt gtgcggaacc cccttcccag ccgtcgaaga
     2101 ccaccgtcag gcggtgccct tttcgttccc ggtaggggat aagtaagcga agcagggcct
     2161 ggcggccctc ttcgagcgtg agacgctccg cccgccggag ctgctccgac tggcggatga
     2221 gattgtaacc atcgatgatt atgtgcatga gagcttgctt ttctattgtc ttgaccgatt
     2281 gagttatgct aatgtttcca cccatacacg atgtcaactt cctcgtaagc aagtgcgtgc
     2341 gtttccgggc cgcgacgggg ctccggatgg gttggaaacc ttatcctttg ttagtactaa
     2401 tcaccgtttc taatcaagtc ctaaaatggc cggtaacctt tatctttggg gggatgcaaa
     2461 atgaaaagat aaaagtaacc ggatagttgc gcttatatgt aaaacctaaa aaagaggagg
     2521 gaattttatg gcagaaaaag tttatgcacc cggcgaaccg tacaagtatc aactgttgat
     2581 caaacacatc ctcgaaatgc ccctggcttt tgccccgaac caggagatcg tttaccggga
     2641 caaggtgcgt cttacctacc ggaccctcaa tgaacggatt caacgcctgg ccaacggcct
     2701 ggaaaagatc ggcgccgggc ccggggaccg gatctgcatc ttcgattacg acagccaccg
     2761 ttatctggag tgcttctttg ccgtccccat gatgggcgcc atcatgcata cccagaactg
     2821 gcgcctttcc cccgagcaga tcctctatac gatgaatcat gcggaggaca agatcgtcct
     2881 gatccatcag gattttgtgc ccctcctcga ggcgatctgg gacaagctgg cgacggtcaa
     2941 aaaggtcatc ttgattaccg acgacggaaa caggccgaca tcaaaagtgc ctttcgacgc
     3001 ggagtacgag gaaatgttgg cgtcgtcgtc gccggcgcat gatttccccg acctggacga
     3061 gaacacccag gcgacgatct tctacaccac cggcaccacc ggtctgccca agggggtttt
     3121 tttctcccat cgccagttgg tcctccacgc ccaggccctt cttttggcca ccggttcgta
     3181 tgcctccatt ggccgctgcc aatccaatga cgtctatatg cccatcaccc ccatgttcca
     3241 cgtccatgcc tggggctttc cctgggcggc caccatgctg ggggtaaagc aggtctatcc
     3301 gggacgttac gaaccggcca tgctcctgaa actcatcctt acggagcggg tcacattctc
     3361 ccactgcgtc ccgacgatca ttcagatgct ggtgtccagc ccggcggcga aacagtacga
     3421 tctttccttc tggaaggtca tcatcggcgg ggcccggctc accaagagct tggccctgga
     3481 ggccatgaaa atgggcatcc agattttcgc cgcctacggg atgtcggaga cctgccccct
     3541 gcttacggcg gccaatctca agccctggat gctgaagaat tccatggaag agaacgtgga
     3601 caccctggtc aagaccggtc tcccggcgcc cctggtatat gtgcgcctca aggatcctct
     3661 gggcaaggat gtgcctcatg acggtgtgtc cacgggagaa gtatgtgtgc ggtccccctg
     3721 gctgacggag agatatttca aggatccgga gcggtccaag gagctctgga cggacggctg
     3781 gctccacacc ggggatgtgg gcttgatcga tgccgagggc tacctccagg tcacggatcg
     3841 catcaaggac gtcatcaaga ccggcggtga atggatttcc tccctggagc tggaaaacat
     3901 catgtccagc cacgaggcgg tcctagaggc ggcggccatc ggtctccccg atgccaaatg
     3961 gggagagcgg cccctcctgg tggtggtttt gaaaccggat tacgccggca aggtcacggg
     4021 tgaagaa
//
LOCUS       DAOSMF010000160         4022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_24240_length_4022_cov_18.835392, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000160 DAOSMF010000000
VERSION     DAOSMF010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24240_length_4022_cov_18.835392"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="PLT63_10330"
     CDS             <1..740
                     /locus_tag="PLT63_10330"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD99158.1"
                     /translation="SAPAKVSYEYMDGKKLADPRFLATMSHELRTPLNSIIGFTGILL
                     QGLVGPLNDEQRKQLQWVRTSANHLLALISDILDISKIEAGKLQLSRETFDLRAAIDK
                     IVQTFRPLTEKKGLRLEAAIAPEVGELYIDARRVEQINLNLLSNAAKFTDAGSITVSC
                     TTEDGKYLIRVADTGIGVKKDQIDEMFKPFRHIDTGLSRKYEGTGLRLSICKKLLELL
                     GGEIGVESEWQRGTIVTVSIPVERRPT"
     gene            737..1129
                     /locus_tag="PLT63_10335"
     CDS             737..1129
                     /locus_tag="PLT63_10335"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOD99159.1"
                     /translation="MKGRILVIEDNEQNLYLVKFILENNGYEVCEARDGREGIEMAEK
                     IKPDLILLDIQLPVMDGYAVARTLRTKPDLAKVPIVAVTSYAMAGDREKAIAAGCNGY
                     IEKPINPDIFMQQVEQQLCLRADGGEGK"
     gene            1196..2023
                     /locus_tag="PLT63_10340"
     CDS             1196..2023
                     /locus_tag="PLT63_10340"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOD99160.1"
                     /translation="MLKTHSFEIMKAENGADALEIARQSPPDLIVSDILIPIMDGYAL
                     CRRCKTDDRLHSVPFVFYTATYTQQEDQALGLDLGADRFLLKPQEPEVLIGIFQELLN
                     KKRRAEAPVLASLEEEMEFLRRHNEALGRKLEKKILDLEAVYEQLRTQEETLRKEDEF
                     LARVIENIPDMIFVKDAKTLNFVRFNRAGEKLLGVRREDMIGKNDYDFFPREQVDFFT
                     AKDRETLAGKKLVDIRDESIETRDLGTRILHTKKIPILDEKVGSALSSGDIGGRHRA"
     gene            2039..2986
                     /locus_tag="PLT63_10345"
     CDS             2039..2986
                     /locus_tag="PLT63_10345"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOD99161.1"
                     /translation="MDRQIYENAVEGVQDGIFQSTPAGAIIMANPAMARNLGYASPEE
                     LMAEMTDVASQLYVRPEDREKILRLIDRHGFVKDYEVQQRRKDGAVFWISLSFQAVRD
                     EEDNILYYEGMSLDTTDGKLSMERIVKSLKATVQALAVAVETRDPCTAGHQRRVAELA
                     GAIAQEMKMDGDQKSGIRMASLIHDLGKISVPAEILSMPRKLSEIEFSLVKTNARSGF
                     EILKDIDFPWPIAEIVLQHHERMNGSGYPRGLKGDKLLIEAKILMVADVVESMISYRP
                     YRPALTLAEIEENQGLLYDPEVVDACLRLFRGKGFRLAV"
     gene            3300..3446
                     /locus_tag="PLT63_10350"
     CDS             3300..3446
                     /locus_tag="PLT63_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99162.1"
                     /translation="MTFLIDKSLYEEVQPITVDFITGAMGSGFKLTSSLASAGGGCGS
                     CSAC"
     gene            3566..>4022
                     /locus_tag="PLT63_10355"
     CDS             3566..>4022
                     /locus_tag="PLT63_10355"
                     /inference="COORDINATES: protein motif:HMM:NF024574.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial transcriptional activator
                     domain-containing protein"
                     /protein_id="HOD99163.1"
                     /translation="MSDNSLPGIHIFTGCEPRKIDDRGGNPRWEDLNSEELQILVEDY
                     LVAGHCGEAIEILRRLVARHPGEDSFVLKLAWAYHDGGFLDEAVACFEALLEKELQRR
                     IFTGFAFDELVRIYKREGRYDNLVAICERAMAAQPEDYALMGDLGDAYLK"
BASE COUNT          962 a         1053 c         1202 g          805 t
ORIGIN      
        1 tttccgcgcc ggccaaggtg agttatgagt atatggacgg caagaaactt gccgatccgc
       61 gttttctggc caccatgtcc catgagttgc ggacgcccct gaattccatc atcggcttta
      121 ccggaatcct cctccagggc ctggtgggac cgctcaatga tgaacagcgc aagcagctcc
      181 aatgggtgcg caccagcgcc aaccatctcc tggcgctcat cagcgatatc ctggacatct
      241 cgaagatcga ggcgggcaag ctccagctct cccgggagac cttcgatctg cgggcggcca
      301 tcgacaagat cgtgcagacg ttccggcccc tgacggaaaa gaagggcctg cgccttgagg
      361 ccgccatcgc cccggaggtg ggggagctgt acatcgatgc gcgccgggtg gaacagataa
      421 atttaaactt gttgagcaat gccgcaaaat ttacggatgc gggaagcatt accgtatcct
      481 gtacgaccga ggatggcaaa tacctgattc gggtggccga caccggcatc ggcgtcaaaa
      541 aggaccagat cgacgagatg ttcaaaccgt tccgccatat agacaccggg ttgagccgca
      601 aatatgaagg taccggcctg aggctgtcca tctgcaagaa gctgttggaa ctcctgggcg
      661 gagagatcgg ggtggaaagc gagtggcagc ggggaacgat tgttaccgta tccatcccgg
      721 tggaaaggag accgacatga aaggcaggat tctggtaatc gaggacaatg aacagaacct
      781 ctatctcgtc aagttcattc tggaaaataa tggctacgag gtgtgcgagg cccgagacgg
      841 ccgggaaggg attgaaatgg ccgaaaagat aaagccggat ctgatcctcc tggacatcca
      901 gcttcccgtc atggacggct atgccgtggc ccggacgctc aggaccaagc cggatctggc
      961 aaaggtcccg attgtggcgg tcacctcgta tgcgatggcc ggggatcggg agaaggctat
     1021 cgcggcgggc tgcaacggct atatcgagaa accgatcaat cccgacatct ttatgcaaca
     1081 ggtagaacaa cagctatgcc tccgggcgga cggcggggaa gggaagtgac caacttatgg
     1141 accggatgct aatcgtcgac gacaatgcgg aaggtctcta tttgctcgac tctacttgct
     1201 gaaaacgcat agttttgaga tcatgaaggc ggaaaacggc gccgacgccc tggagatcgc
     1261 ccgccaatcg ccgccggacc tcattgtcag tgacatcctc atacccatta tggacggtta
     1321 tgccctctgc cgccgctgca agacggatga tcgcctgcat agcgttccct ttgtatttta
     1381 cacggccacc tatacccagc aggaggatca ggcgctcggc ttggatctcg gcgccgaccg
     1441 cttcctcctc aagccccagg aaccggaggt cctgataggc atcttccagg aattgttgaa
     1501 caaaaagcgc cgggcggaag caccggtgct agcatccctg gaggaggaga tggaatttct
     1561 ccgccgccac aacgaagccc tgggccgcaa actggagaag aagattctgg atctcgaagc
     1621 cgtgtatgag caactgcgga cccaggagga gacgctgcgg aaggaggacg agtttctggc
     1681 ccgtgtcatc gagaacatcc ccgacatgat ctttgtcaag gacgcaaaaa ccctgaactt
     1741 tgtccggttc aaccgggccg gggagaaact gctgggtgtg cgccgggagg atatgatcgg
     1801 caaaaacgat tacgattttt tccccagaga gcaggtggat ttctttaccg ccaaagaccg
     1861 ggagaccctc gccgggaaga aactggtgga cattcgcgac gagtccatcg agacccggga
     1921 tctgggaacg cggatcctcc atacgaagaa gatccccatc ctggatgaaa aggtgggaag
     1981 cgctttatct tctggggata tcggaggacg tcaccgagct tagggcagcc gaaaagagat
     2041 ggatcgccag atctatgaaa acgccgtcga gggcgtccag gacgggatct tccagtccac
     2101 gccggcgggg gccattatca tggccaaccc cgccatggct cgaaacctgg gctatgcgtc
     2161 tccggaggaa ctgatggccg agatgaccga tgtggcttcc cagctctatg tccgcccgga
     2221 ggaccgggag aagatcctgc gtctcatcga taggcatggt tttgtcaaag attacgaagt
     2281 tcagcagcgc cggaaagacg gtgccgtttt ctggatatcg ctttccttcc aggccgtccg
     2341 ggacgaggag gacaacatcc tctattacga aggcatgagc ctggacacaa ccgatggcaa
     2401 gctgagcatg gagcgcatcg tcaaatcatt gaaagcgacg gtgcaggcat tggccgtggc
     2461 ggtggaaaca agggatcctt gcacggctgg tcatcagcgc cgcgtcgccg aactggccgg
     2521 tgccattgcc caagagatga aaatggacgg cgatcaaaaa agcggcatcc gcatggccag
     2581 tctcattcac gatcttggta agatatctgt tccggcggag atcctcagca tgccgagaaa
     2641 gctgtcggaa atcgagttca gtctcgtgaa gaccaatgcc cgttccggct tcgagatcct
     2701 aaaggatatc gattttccct ggcccatagc ggagatcgtc ctccagcatc acgaacgcat
     2761 gaacggctcg gggtatcccc gtggcctgaa aggagataaa ctgcttatcg aggcgaagat
     2821 tctcatggtg gccgacgtgg tggaatctat gatctcttac cgtccctacc gtccggcact
     2881 gaccctggcg gagatcgaag agaaccaggg cctcctttac gatcccgagg tcgtggatgc
     2941 ctgcctgcgc ctgttcaggg gaaagggttt ccggttggcg gtttgattgt ttgcgaaagt
     3001 aatatcacac ctgccgtcgg ctaggctaag gataaccgga cggacagtaa atatatttcc
     3061 gtagtttatg attgacaaac gctggaccca tatataggtt agcgatccga taagatttta
     3121 caaggaggag caaaatgttt caagtgactg aaaaagcgat ggaagtcatc aaggaattcc
     3181 tgcagaacaa ggctatccaa acgatccgga tcatgatgac agccggttga ggcggcccca
     3241 atctggggat ggctctggat gagccaaaag acaccgatga agtgatacag gataacggct
     3301 tgaccttcct gatcgacaag tccctttacg aagaggtaca gccgatcacc gtagatttca
     3361 ttaccggggc catgggatca ggattcaagc tgacctccag tctggcctcg gccggcggcg
     3421 gatgtggttc ctgctccgcc tgctgagtcg gggtggcatg ggctgaaaca agtttaaaca
     3481 caggccccga cttgtagcca gggccttttt ccgtaggaag gatggcaagg ttcttttcac
     3541 cattgcctac ggtacgacga ggaagatgag tgataattcc ctgcctggga tccacatatt
     3601 tacgggttgc gaaccgagga agatagatga taggggcggc aacccccggt gggaggatct
     3661 gaattccgaa gaacttcaaa tccttgtgga agattatctg gtggcgggac attgcggtga
     3721 agcgatcgaa atcttacggc ggctggtggc tcgccatccc ggcgaggact cctttgtcct
     3781 gaagctagcc tgggcctatc acgacggcgg gtttctcgat gaggcggtgg cctgctttga
     3841 agccctgctg gaaaaggagc ttcagcgccg gatatttacc ggctttgcct ttgacgaact
     3901 ggtgcggatc tacaagcggg aaggacgcta tgacaatctc gtggcaatct gcgagcgcgc
     3961 catggccgcc cagccggaag actacgccct gatgggggac ctgggagatg cctatctgaa
     4021 ga
//
LOCUS       DAOSMF010000161         4018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_24264_length_4018_cov_18.656321, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000161 DAOSMF010000000
VERSION     DAOSMF010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4018
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24264_length_4018_cov_18.656321"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..667)
                     /gene="kdpC"
                     /locus_tag="PLT63_10360"
     CDS             complement(95..667)
                     /gene="kdpC"
                     /locus_tag="PLT63_10360"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF001454.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HOD99164.1"
                     /translation="MLHLIRTAVTIFTMIGLITGVVYPLAATGLAQLFFPWQANGSII
                     KIGGGKAASELIGQPFRSNGYFWGRPSATAPGPYNAAASTGSNLGPSHPALRASVKRR
                     ITTLQAAAPENRLPIPVELVTASGSGLDPHISPAAAFYQVKRVAKARGMEEGKVADLI
                     RIHIRDRRFGIFGEPVVSVLRLNMALDAIP"
     gene            complement(684..2750)
                     /gene="kdpB"
                     /locus_tag="PLT63_10365"
     CDS             complement(684..2750)
                     /gene="kdpB"
                     /locus_tag="PLT63_10365"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HOD99165.1"
                     /translation="MKTKAKSFSLADPRIIRTAFVDAFRKLNPLHLIKNPVMFVVEVG
                     CILTTLLYFQALTGRGEEPVSFIMAISLWLWFTLLFANFAEAMAEGRGKAQARSLKST
                     RRDVTSKRLQEPRRDALIDVIPGSRLKKGNIILVEAGEIIPGDGEVTVGIASVDESAI
                     TGESAPVIRESGGDRSAVTGGTRVLSDWLIIHITANPGETFLDKMIAMVEGARRQKTP
                     NEIALDILLAGLTLVFLLATATLVPFSLYSVRATGQGAPLSLTVVIALLVCLIPTTIG
                     GLLSAIGIAGMDRMTQANVIAMSGKAVEAAGDVDVLLLDKTGTITLGNREAAEFVPVA
                     GIKEQQLADAAQLASLADETPEGRSIVVLAKQQYGIRERDLKSLGARFIPFTAMSRMS
                     GVDLDGREIRKGAADAIATYLQARGGKFPEKMMAAVREIAGQGSTPLVVTEGANVLGI
                     VRLSDVVKGGIRERFSELRRMGIRTVMITGDNPLTAAAVASAAGVDDFLAEATPETKL
                     KMIRDYQAQGHLVAMTGDGTNDAPALAQADVAVAMNTGTQAAKEAGNLVDLDSNPTKL
                     IEIVAIGKQLLMTRGTLTTFSIANDVAKYFSILPAAFSGIYPGLAALNIMSLATPQSA
                     VLAAVIFNALVIVALIPLALRGVSYRPMGAAAVLRRNLIIYGAGGLAAPFLGIKAIDM
                     ILTILL"
     gene            complement(2764..>4018)
                     /gene="kdpA"
                     /locus_tag="PLT63_10370"
     CDS             complement(2764..>4018)
                     /gene="kdpA"
                     /locus_tag="PLT63_10370"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HOD99166.1"
                     /translation="ANTIGNVWVDLTRSLIYIMLPLSLVMALFLVSQGVVQTLHGPVK
                     VQLLDATADEKWQPAPERIIPLGPAASQVAIKQLGTNGGGFFNANAAHPLENPTPLSN
                     FIEMLAILLIPAALCLTFGGMIGDRKQGWALLAAMMLLLLPLLGVSLYSEQEGNVYLR
                     QMNVDQNISAWQSGGNMEGKEVRFGITNSALWAVATTAASNGSINAMHDSFTPLGGLA
                     PLMLIQLGEIVFGGVGSGLYGMLMFVIITVFVAGLMVGRTPEYLGKKIEAHEVKMASL
                     VILIPAAVVLIGTAVAVVTKAGLSGIANPGPHGFSEILYAFSSAANNNGSAFAGLNAN
                     SLFYNTALGLAMLIGRFWLMIPVLAVAGSLARKKLVPAGAGTLPTHIPLFVVFLVAVI
                     ILVGALTFIPALALGPLAEHLARMG"
BASE COUNT          774 a         1186 c         1264 g          794 t
ORIGIN      
        1 caggagttca tcggggtccg gtcttttcgc ttccatgtct ttgccgttct caaaacgatc
       61 atgtccataa tgcctcgacc atattctagc agaatcatgg tatcgcatcc agtgccatgt
      121 tcaggcgcag gacggacacc accggttcgc cgaagatgcc gaaccgcctg tcccggatgt
      181 gtatccggat caggtcggcg actttccctt cttccattcc ccgggctttt gccacgcgct
      241 tcacctggta aaacgcagcg gcgggactga tatgcggatc gagtccgctc ccggacgcgg
      301 taaccagctc cacggggatg ggcagccggt tctccggagc ggcggcttgc agagtcgtga
      361 tgcgtcgctt tacggacgcc cgcagggcgg gatgggatgg ccccagattt gaaccggtgg
      421 aggcggcggc attgtaaggt ccgggcgctg tagcggaagg ccttccccag aaatagccgt
      481 tactgcggaa aggttggccg atgagttccg aggcggcctt cccgccgccg atcttgatga
      541 tgctgccgtt cgcctgccag gggaagaaca gttgtgccag gcccgttgcc gccaggggat
      601 agacaacccc cgtaatcagg ccgatcatgg taaagatggt gactgccgtt cttatcaggt
      661 gtaacatggc cgttcctttc cgctcagagt aggatggtca gaatcatgtc gatggccttg
      721 attcctaaaa agggcgccgc cagtccgccg gcgccgtaaa tgatcagatt ccggcgcagg
      781 acggcggcgg cgcccatggg ccgataggag acgccgcgca gcgccagggg aatcagggcg
      841 acaatgacca gggcgttgaa gataaccgcc gcaagaactg cgctttgcgg cgttgccagg
      901 gacatgatgt tcagtgccgc caggcctgga taaattcccg agaaggcggc gggcagaatg
      961 gagaaatatt tggcaacgtc attggcgatg ctgaaggtcg tcaacgtccc ccgcgtcata
     1021 aggagctgtt tgccgatcgc gacaatctcg atcagcttgg tgggattgga gtcgagatcc
     1081 accaggtttc ccgcctcctt tgccgcctgc gtccccgtgt tcatggccac ggccacgtct
     1141 gcctgggcca gcgccggcgc atcgttcgtg ccgtcacccg tcatggctac cagatgcccc
     1201 tgggcctgat aatcgcggat cattttcagc ttcgtctccg gcgttgcctc agccaggaaa
     1261 tcatcgacgc ctgccgcgga ggcgacggcc gccgccgtga gggggttgtc gcccgtgatc
     1321 atcaccgtgc ggatgcccat gcgccgcagc tcggagaagc gctcgcggat gccgcccttg
     1381 actacgtcgc tcaagcggac aatccccagc acattggcgc cttccgtcac caccaggggc
     1441 gtacttccct gcccggcgat ttccctgacg gcggccatca ttttctccgg gaatttgccg
     1501 ccccgggcct gcaggtaggt ggcgatggcg tcggcggccc ctttgcggat ttcccgtccg
     1561 tcgagatcga cgccgctcat gcggctcatg gcagtgaaag gaataaagcg cgcccccaat
     1621 gacttgaggt cgcgttcccg aatgccgtat tgctgttttg ccaggacgac gatgcttctt
     1681 ccctccggag tttcatcggc cagcgaggcg agttgcgccg catccgcaag ttgctgttcc
     1741 tttattcccg ccaccggcac aaattcggct gcctcgcggt tccccaaggt aatggtgccg
     1801 gtcttgtcga ggagcaggac gtccacatcc ccggcggctt cgacggcctt tccggacatg
     1861 gcgatgacgt tggcctgagt catccggtcc atgccggcga ttccgatggc ggagaggagg
     1921 ccgccgatcg tggtgggaat gagacagacc agcagggcga tcactactgt caagctgagg
     1981 ggcgcccctt gtccggttgc ccggacgctg taaagggaaa agggaacgag ggttgcggtg
     2041 gccagcagaa aaacgagcgt aagaccggca aggaggatgt cgagggcgat ctcgttcggc
     2101 gttttctggc gtctggcccc ttccaccatg gcgatcatct tgtcgaggaa tgtttctccc
     2161 gggttggcgg tgatgtggat gatcaaccag tccgacagca cccgggtgcc gccggttacg
     2221 gcgcttctgt cgccgccgct ttcgcggatc accggggcgc tctccccggt gatggcgctt
     2281 tcatcgacag aggcgatacc gaccgtcacc tcgccgtcgc cggggatgat ttccccggct
     2341 tccaccagga tgatattgcc ctttttcagt cgcgaaccgg ggatgacatc gatcaaggca
     2401 tcccggcgtg gttcttgaag acgcttggat gtcacgtccc gccgggtgct tttcagcgat
     2461 cttgcctggg ccttccccct tccttccgcc atggcttccg cgaagttggc gaaaagcagg
     2521 gtgaaccaca gccagagact tatggccata atgaaagaga caggttcctc gccgcgcccg
     2581 gttagggcct gaaagtaaag cagggtggtc aggatgcagc cgacttccac gacaaacatc
     2641 accgggttct tgatcaggtg cagcggattc agcttgcgga aggcgtcaac gaaggccgtc
     2701 cggattatcc gcgggtcggc aagggagaat gattttgcct ttgtcttcat gttgtcctcc
     2761 ttgtcatccc atccgggcca ggtgttcggc gaggggtccc agggccaggg ccgggatgaa
     2821 ggtcagcgct cccaccagga tgattaccgc cacgagaaac actacaaaca gcgggatatg
     2881 ggtgggcagc gtgccggcgc cggcggggac gagtttcttg cgggccaggg aaccggcaac
     2941 ggccaggacg gggatcatga gccagaagcg cccgatgagc atggccaggc caagggcggt
     3001 gttataaaaa agcgagttgg cgttcaatcc ggcgaaggcg ctgccgttgt tgtttgccgc
     3061 ggaagagaag gcgtagagaa tctcggaaaa gccgtgagga ccggggttgg cgattccgga
     3121 cagccccgcc ttggtgacga cggcgacggc cgtaccgatc aatacgaccg ccgccgggat
     3181 gagaatgacg agggaggcca tcttgacttc gtgggcctcg atcttcttgc cgaggtattc
     3241 aggggtgcgg cctaccatga gccccgccac gaagacggtg ataatcacga acatgagcat
     3301 gccgtacaag ccggaaccta caccgccgaa gacaatctct cccaactgaa tcaacatgag
     3361 cggggcaagg ccacccaggg gggtgaaaga atcgtgcatg gcgttgatgc tgccgttgga
     3421 ggcagccgtc gtcgccaccg cccagagcgc ggaattggtg atgccgaacc ggacctcttt
     3481 gccttccatg ttgccgcctg attgccatgc gctgatattc tgatcgacat tcatctgccg
     3541 caagtaaaca ttgccctcct gttcgctata caggctgacc cccagcaggg gcaggagaag
     3601 gagcatcatc gcggcaagca gggcccatcc ctgtttcctg tccccgatca tcccgccgaa
     3661 ggtaaggcag agcgccgccg ggataaggag gatggccagc atctctataa agttggatag
     3721 aggagtggga ttttccaggg gatgggccgc gttggcgttg aagaaccctc cgccgttcgt
     3781 acccaactgt ttgatggcca cctggcttgc cgccggtccc agggggatga tccgttccgg
     3841 cgccggctgc catttttcgt ccgccgtcgc atccagaagc tgaaccttaa ccgggccgtg
     3901 cagtgtttgc acgaccccct gggaaacgag gaacagcgcc ataaccaggg aaaggggcaa
     3961 cataatgtaa ataagcgaac gggtcagatc cacccataca ttgccgatgg tattggcg
//
LOCUS       DAOSMF010000162         3988 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_24489_length_3988_cov_24.210781, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000162 DAOSMF010000000
VERSION     DAOSMF010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3988
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24489_length_3988_cov_24.210781"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="PLT63_10375"
     CDS             <1..632
                     /locus_tag="PLT63_10375"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HOD99167.1"
                     /translation="IAVLDHLFILGDFFDFWFSRRDKIYPGFREIVAVLKDLQAGGVA
                     IHFCEGNHDFFLSGYFSRFQGMNVYEDWTALNLGGKKALIGHGDLVDETNHRYLRLRR
                     ILRSRTFFVLQRLLPLPLLWTIARGSSQVSQEYMGGAKEVIAAKMADFARDRFREGFD
                     AVILGHCHHAELQEGVVAGRKTVFATLGDWITGRDYLHYHDGGFHLCRY"
     gene            713..1561
                     /gene="speB"
                     /locus_tag="PLT63_10380"
     CDS             713..1561
                     /gene="speB"
                     /locus_tag="PLT63_10380"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HOD99168.1"
                     /translation="MNFGALDPECAAYDSALFVVIPVPYDLTSTYQPGSRQGPGAILA
                     ASANMELYDDELGKSTYRQGIYTTPHVEVDARGPEHMMAAIRRAVADVLRDDKIPVLL
                     GGEHSVTLGAVQAVKAAFPGLQTLQLDAHADLRESYQGTPYSHACIARRIQELCPLVQ
                     AGIRSMSEEEALFLRSGAVKTFSADFILDDPDWAEKICTHLDGDVYVTIDLDVLDPSC
                     MPATGTPEPGGVSWRHLLMLLRPLAKKCKIRGLDVVELSPLPGQVAPDFLAAKLIYRI
                     MGYISG"
     gene            1630..3852
                     /locus_tag="PLT63_10385"
     CDS             1630..3852
                     /locus_tag="PLT63_10385"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD99169.1"
                     /translation="MTENLQVSYISVIPPVLGFFVLTGLSLLSIFRGGRNPTNLLFAA
                     ICFMGALINADVALVNLIADKGLAIMIDRFTYVVFVFSLPIYIQFVHHFLGIRKRRWL
                     EYAAYGCSIVFLVLTPTDLFIKGFAVFDFGIIAQAGRAFHWFSVLVSFTVCYCLYTLF
                     TGMKRTRDNRKRNRIKYILWGLGLSSFLISLNILPVSGFNMYPMGNFSFIPAVILAFG
                     VLKYDLLDMGAIIRRGTIYVLLTMVLTIFYVILIALVNALLMWTGESHPVLLPLLLAL
                     FMVVLFNPVREKVRFFIDRLFFRGKYDYQRLLKEVSGQMTRLLRIDELMNLLLTTVEA
                     SLKPTHARLFTVNAQGGFLELRWRGGRDASAASPLMEIPGDHPLLAYLGETKRPLADT
                     LPADAGLQEEETAAVAELIKNRESALILPMFSKEKLIGILDLGQKKSGELYVHEDVEL
                     LATLANQSATALENARMYDELENLNRELERKVEARTADLRQALAEKERTQQQLIQSES
                     LAAIGQLVAGTAHELNNPLASASSLLQSSAETIRDWPEGLRDRDEVIDDLFFSLKELK
                     RAGDIVKSLLDLSRQTQTYMEPVDINTAVDDALRVLFNQFKNLPVTIEKVYEEGLPPV
                     EGNYANLGQVFINIIKNALQALPDGKGTVRISTGHLRERDAVFFECRDTGVGMTAGQR
                     NDIFKPFFTTKPVGKGTGLGLYISHEIVKRHGGEITVASDPGKGSVFTVELPCQRREK
                     "
BASE COUNT          822 a         1088 c         1171 g          907 t
ORIGIN      
        1 ggatcgcggt cctcgatcat ctttttatcc tcggggattt cttcgatttc tggttcagcc
       61 gccgggataa gatatacccg ggattcaggg aaatcgtcgc cgtattgaag gacctccagg
      121 ccgggggcgt tgccattcat ttctgtgagg gcaatcacga ttttttcctg tccgggtatt
      181 tctcccgctt ccaaggtatg aacgtctatg aagactggac ggcccttaat ctgggcggga
      241 aaaaggccct tatcggtcat ggcgatctgg tggatgagac aaaccatcgt tatctccgct
      301 tgcggcggat tcttcggagc aggacattct tcgttctgca gcggctcctg cccctgcccc
      361 tgctttggac gatcgcccgc gggagttccc aggtcagtca ggagtatatg ggcggcgcca
      421 aggaggttat tgccgctaag atggcggatt tcgcccggga tagattccgg gagggctttg
      481 acgccgtgat cctcgggcat tgccaccacg ctgagcttca ggaaggtgtg gtggcgggac
      541 ggaagactgt cttcgccaca ctgggggact ggatcacggg tcgcgactat ctccattatc
      601 atgacggggg ctttcatctg tgccgctatt gatgataggg caacaggata acactggaca
      661 ggccatcggg ggtatgttaa gcatgcccat cccgaaccac aaggagtgaa ccatgaattt
      721 cggcgccctc gaccccgaat gcgcagcata cgacagcgcg ctttttgtcg ttatccctgt
      781 tccctacgat ctcacctcta cttaccagcc cggttcccgc cagggtcccg gggccatcct
      841 ggcggcatcg gccaatatgg aactatatga tgacgagctc ggaaagagca cctaccgaca
      901 gggcatttat acgacccctc acgtggaggt ggatgcccga ggccccgagc acatgatggc
      961 ggccatccgc cgggcggtgg ccgatgtctt gagggacgac aagataccgg tactgctggg
     1021 gggagagcac agcgtgactt taggcgccgt ccaggccgtc aaggcggcgt ttcccggcct
     1081 gcaaacgctg caactggatg cccatgcgga cctgcgggag tcctatcagg gcactcccta
     1141 cagccacgcc tgcatcgctc ggcggattca ggagctctgc cccctggtcc aggcgggtat
     1201 tcggagcatg agtgaagagg aggccctctt tctgcggagc ggcgccgtca agaccttttc
     1261 cgccgacttc atcctcgacg accctgactg ggccgaaaag atctgcaccc acctggacgg
     1321 ggatgtctac gttaccattg atcttgacgt cctggacccg tcctgcatgc cggcgacggg
     1381 aaccccggag ccgggaggcg tttcctggcg ccacctcctc atgctgctgc gtccgctggc
     1441 aaaaaagtgt aaaattcggg gcctcgacgt cgtggaattg tcgccacttc ccgggcaggt
     1501 ggcccctgat ttcctggcgg ccaaactcat ctatcggatc atgggttata taagcggtta
     1561 ggacgcgatc ccgcggacaa atattctgtt gtccactgag cgaaacaggt acagacaggc
     1621 agaggcgcga tgaccgaaaa tctgcaagtc tcctatattt ccgtaatccc gccggtgctc
     1681 ggcttctttg tcttgacggg cctgtccctc ctttccatct tccggggcgg aagaaatccc
     1741 accaaccttc tgtttgccgc gatctgtttc atgggcgccc tgatcaacgc cgacgtggcc
     1801 ctggtgaatc tgattgccga taaaggactg gctatcatga ttgaccgttt cacctatgtt
     1861 gtttttgtct tcagcctgcc catctatatc cagttcgtgc atcatttcct cgggatcagg
     1921 aaacgccgct ggcttgaata cgcggcctac ggctgcagca tcgtatttct cgtattgacg
     1981 ccgacggatc ttttcatcaa gggcttcgcc gtgttcgact tcgggatcat cgcccaggcg
     2041 gggcgtgctt ttcactggtt ttccgtcctg gtgagcttta cagtctgtta ctgtctatat
     2101 actctcttta ccgggatgaa gcggacccgg gacaaccgga aacgcaatcg tatcaaatat
     2161 attctgtggg gtcttggcct cagctcattc ctcatatctc tgaacatcct gccggtaagc
     2221 gggttcaaca tgtatcccat ggggaatttc agtttcatcc ccgccgtcat cctggccttc
     2281 ggggtcctta aatacgatct actcgacatg ggcgcgataa tacgccgggg gacgatatat
     2341 gttctcctca ccatggtttt gaccatcttt tatgtgatcc tcattgccct ggtcaatgcc
     2401 ctgctgatgt ggacggggga aagccatccg gtcctcctgc cgttgctgct ggcgctgttc
     2461 atggttgtgc tgttcaaccc cgtgcgggag aaagtgcggt ttttcatcga caggctgttt
     2521 ttccggggca agtatgatta ccagcgtctc ctgaaggagg tcagcggcca gatgaccagg
     2581 ttattgcgga tcgatgagct gatgaatctg ctccttacca cggtggaggc atctctcaaa
     2641 cccactcatg cccgtctttt caccgtcaac gctcagggag gtttcctcga attgcggtgg
     2701 cggggaggaa gagatgcttc cgcggcgtct ccgttgatgg agattcccgg tgatcatccg
     2761 ttgcttgcgt atctggggga gacaaaaaga ccgctcgcgg ataccctgcc ggccgatgcg
     2821 ggtctgcaag aggaagaaac cgcggccgtt gcggagttga tcaagaacag ggaatcggcg
     2881 ttgatcctgc ccatgttttc caaggaaaag ctgatcggca tcctcgatct ggggcagaaa
     2941 aagtccgggg agctttatgt tcacgaggat gtggaacttc ttgccacctt ggccaatcag
     3001 agcgccacgg cgctggaaaa tgcccggatg tatgatgaat tggaaaatct caaccgggag
     3061 ttggaaagaa aagtcgaagc gcggaccgca gacctccgcc aggccctggc agagaaggag
     3121 cggacccagc agcagctaat ccagtcggag agtctggcgg cgatcggcca gctcgtcgcc
     3181 ggcacggccc acgaactgaa caatcccctg gccagcgctt ccagtctcct gcaaagcagc
     3241 gctgaaacca tccgcgactg gccggagggc ttgcgggatc gggatgaagt gatcgacgat
     3301 ctttttttct ccctgaaaga gttgaagcgg gccggggaca tcgttaaaag cctgctcgat
     3361 ttgtcgcgtc aaacccagac ctacatggag ccggtcgata tcaatacggc ggtcgatgac
     3421 gccttgcggg ttcttttcaa ccagttcaag aacctgcccg tgacaatcga gaaggtttat
     3481 gaagaggggc tgcccccggt ggagggtaat tatgccaacc tgggccaggt cttcataaac
     3541 atcatcaaga acgccctgca ggcgctgccg gatggtaaag gtacggtccg gatcagtacc
     3601 ggccacctcc gggagcgcga tgccgttttt tttgaatgcc gggatacagg ggtggggatg
     3661 acggcggggc agcgcaacga tatttttaaa ccgtttttta ccaccaagcc ggtgggcaag
     3721 ggaaccggac tgggactcta catatcacat gaaatagtga agcggcatgg cggtgaaatt
     3781 accgtcgcca gcgatccggg gaagggatcg gtctttacag tcgagcttcc ctgccagagg
     3841 agggagaaat gattgttctg atcgtcgatg atgaggaagg ggtgcgcaga tcgttgcgga
     3901 aggtcctgga aagggatcaa tatgggattc tagcggcgga aaacggccag gaagccatcg
     3961 agatcgtaag ggagaacaaa cgtgatat
//
LOCUS       DAOSMF010000163         3973 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_24621_length_3973_cov_25.539561, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000163 DAOSMF010000000
VERSION     DAOSMF010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3973
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24621_length_3973_cov_25.539561"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            281..1117
                     /gene="lsrF"
                     /locus_tag="PLT63_10390"
     CDS             281..1117
                     /gene="lsrF"
                     /locus_tag="PLT63_10390"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: protein motif:HMM:NF006081.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HOD99170.1"
                     /translation="MDWGMENRLSQLIQADGHCFFLPIDHGYFQGPTSCLEKPGETIK
                     PLLPYCDALFVTRGVLRAAVNPVGSKPIILRVSGGTSVLGEDLANESLTTSIEEILRL
                     NAAAVGISIFVGSDYERESLMNLSEIVNQCEDFGIPVMAVTAVGKELEKRDARYLALA
                     SRIAAELGARVVKTYWCKDFDKVTCGCPVPVVMAGGPKCETEKEVMDFVYDGMQNGAV
                     GLNLGRNVWQNPHPVAMMRALHGIIHGNLNAAAAWELFNDLKGEKIKRGKSGGKKVQG
                     KQ"
     gene            1128..2159
                     /locus_tag="PLT63_10395"
     CDS             1128..2159
                     /locus_tag="PLT63_10395"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOD99171.1"
                     /translation="MRVAMYYRNDDVRVEELTVPAIGPGEMLVKVMASGICGSDIMEW
                     YRMKKAPLVLGHEIAGTVVAVGAGVTAYREGDRVFVSHHIPCNTCRYCLQGCHTACET
                     LHGTNYDPGGFTEYLRVPHLNVDRGTFLLPGNVSFAAGAMIEPLACVIRGQRLARLQP
                     GCSVLILGSGISGILHVMLARATGAGNIMATDISAHRRDQAVRFGADAVADARDDIPA
                     WVRQINEGRLADLVIVCTGAPVAFAQSLQCVDRGGTILCFATTEPGIDLAVPINDFWR
                     NEIKLMPSYGNSPQDAVAAIELIRLGRVPVERMITHRLPLTEAQQGFRLVADGGEALK
                     VILLPHGQG"
     assembly_gap    2215..2314
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2341..3615
                     /locus_tag="PLT63_10400"
     CDS             2341..3615
                     /locus_tag="PLT63_10400"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOD99172.1"
                     /translation="MDGLGLFIRYEEKLIRASLAADGETLLLGAAAPPPVHSDPLPTR
                     QSPAGVIIAPSAPLKRGNGMIWNRADSRTATLAKTARRLGRGAILAAAPPEPYRSVIR
                     YLAAAPDGIIRPQDFETRVFLTDLPVWKTGGEEELTERLEKRRVVVTPDGTIVATAKR
                     DLDTAIVIYSAVCFAVFVKFFFDLLPRPGHRPATDETTGALVAKIRPHLDPPSRSVPP
                     PETAAPLPTGPFETEAAIRTAIAATGRRTVAAGLVNASFGNISYRRGDELFITGRGSA
                     LDDLEGGIITVPLPGPSPQDAGASTELPAHRRIVLETPYRAVLHGHPPFCVILSLDCR
                     EYDCPQRGGCHRFCPRERRIAGFAVVTGEAGGGPYGLGRTVPPAVRETGAAIVYGHGV
                     FTAGRDDFRDAFRLLKELETTCRRLFFRMIEF"
     gene            complement(3623..>3973)
                     /locus_tag="PLT63_10405"
     CDS             complement(3623..>3973)
                     /locus_tag="PLT63_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOD99173.1"
                     /translation="CKRAADATLEDLLTADGLAVGTPENFGYMSGMIKDFFDRTYYPA
                     QDKVFRKPYVVFISAGNDGTGALKAIERIALGYKFKPVYNPVIARGQVTPEILAQCRE
                     LGGVLAGGCQAGIF"
BASE COUNT          736 a         1236 c         1158 g          743 t
ORIGIN      
        1 cgttgaactt gccgtcctga acggaactga ctacgtacag gccgtaactt atcttccgaa
       61 aggcttctct gttcatgact tcctgctcct gttgttattt taccgccggc ggttgccgtg
      121 gaaatccttg tagtcgacat ttctcaagca tcatatatga ttttcctgcc atataccagc
      181 gaaaatccgg ccccatcggc gactcggcaa ttccccacta tccttgcggc ggggaatatg
      241 atatgctcgt cttaaaagaa acatttgggg gaggaagatt atggactggg gaatggaaaa
      301 ccgcctgtcg cagctcattc aggccgacgg gcactgcttt tttttaccca tcgaccacgg
      361 ttactttcag gggccgacct catgtttgga aaaaccgggg gaaaccatca aaccactgct
      421 tccatactgc gacgccctgt tcgtcacccg tggggtcctg cgggccgccg tcaaccccgt
      481 gggcagcaag cccatcatcc ttcgggtgtc cggagggacc agtgtcctgg gagaagattt
      541 ggccaatgaa tccctgacga cctccatcga ggaaatcctg cgcctgaacg ccgccgccgt
      601 ggggatatcg atcttcgtgg gcagcgatta cgaacgggaa tcccttatga atctgtcgga
      661 aattgtcaat caatgcgagg atttcggcat tcccgtcatg gccgtcaccg ccgtggggaa
      721 agaactggaa aaacgggacg cccgctatct ggccctggcc tcacgcatcg ccgccgaact
      781 cggggcccgg gtggtgaaaa cctactggtg caaggatttc gacaaggtca cttgcggctg
      841 tcccgtaccc gtcgttatgg cgggtggacc caagtgcgaa accgaaaagg aggtcatgga
      901 cttcgtttac gatgggatgc aaaacggagc cgtgggactc aacctgggac gaaacgtctg
      961 gcagaacccc catccggtgg cgatgatgcg ggccctgcac ggaatcattc acggcaatct
     1021 caacgccgcg gcggcctggg agctcttcaa cgacctcaaa ggggaaaaga taaaaagagg
     1081 gaaaagcggc gggaaaaagg tgcagggcaa gcaatagaga aggaaccatg cgcgtcgcca
     1141 tgtattatcg caacgatgat gtccgggtcg aggagctgac cgtcccggcc atcggccccg
     1201 gtgagatgct ggtcaaggta atggccagcg ggatctgcgg cagcgacatc atggagtggt
     1261 accggatgaa gaaggccccc ttggtgttgg gccacgaaat cgccgggacc gtcgtcgcgg
     1321 tcggcgccgg ggttaccgcc taccgcgagg gcgaccgggt cttcgtctcc caccatatac
     1381 cctgcaacac ctgccggtac tgcctccagg gctgtcatac cgcctgcgaa accctccacg
     1441 gcaccaacta cgaccccggc ggtttcaccg aatatttgcg ggttccccac ctcaacgtcg
     1501 atcgggggac gttcctcctg cccgggaacg tctccttcgc cgccggggcg atgatcgagc
     1561 ctttggcctg cgtcatcagg ggacagcgcc tagcccggct ccagcccgga tgttccgtgc
     1621 tgatcctggg cagcggcatc tccggaattc tccacgtcat gctggcccgg gctaccggag
     1681 cgggaaacat aatggccacg gacatcagcg cccatcgtcg ggatcaggcc gtccgcttcg
     1741 gcgccgacgc cgtggctgac gcccgtgacg atattccagc ttgggtgcgg cagatcaacg
     1801 aaggccgcct cgccgacctg gtcatcgttt gcaccggcgc cccggtggcc ttcgcccagt
     1861 ccctccagtg tgtggaccgg ggaggaacga tcctctgctt cgccaccacg gagcccggta
     1921 tcgatctggc cgtgccgatc aacgacttct ggcgcaacga gatcaagctc atgccctcct
     1981 acggcaacag cccccaggac gccgtggcgg ccatcgagct gatccgcctg ggtcgtgtcc
     2041 ccgtagagcg gatgatcacc catcgtctac ccctgacaga ggcccagcag gggttccggc
     2101 tcgtggcgga cggcggcgaa gccctgaagg tgatcctcct tcctcacggg caagggtgac
     2161 ggcccggaga tgatctcctg agcatatgga tggattaggg ctttttatcc gctannnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncggccc ggagatgatc tcctgagcat
     2341 atggatggat tagggctttt tatccgctac gaggaaaaac tgatccgggc gagccttgcc
     2401 gccgacgggg agacattgct cctgggcgcg gcagcgcccc ctcccgtcca ttcagatcct
     2461 cttcctacgc ggcaatcccc cgccggagtc atcatcgccc catctgctcc cctaaaacgg
     2521 ggaaacggga tgatctggaa ccgggcggat tcccggacgg caacgctggc taaaacggcc
     2581 cgccgcttgg gtcgcggggc gatcctcgcc gccgccccgc cggagccata ccggtccgtc
     2641 atccgctacc ttgccgccgc cccggacggc atcatccggc ctcaggattt tgaaacccgg
     2701 gtctttctca ccgacctgcc cgtatggaaa accggcgggg aggaagagct gacggagagg
     2761 ctggaaaagc gccgggtcgt cgtcactccg gacgggacga tcgtcgccac ggccaagagg
     2821 gacttggata cggcaattgt catatacagc gccgtctgtt tcgctgtttt tgtcaaattt
     2881 ttctttgatc tgctgccgcg cccggggcat cggccggcaa cggatgaaac gacgggcgcc
     2941 ctggtcgcga aaatccgtcc ccatctcgat cccccctccc gaagcgtccc tccgccggaa
     3001 acggccgccc ccctcccgac cgggcccttc gagacggagg cggcaatccg taccgccatc
     3061 gccgctactg gacgcaggac cgtggcggcg gggctggtaa acgccagctt cggcaatatt
     3121 tcctatcgcc ggggcgatga gcttttcatc accggccggg gaagcgccct ggacgacctg
     3181 gagggcggaa tcatcaccgt cccgctgccg gggccgtcac cgcaagacgc cggcgcttcg
     3241 acggaactcc ccgcccaccg gcgcatcgtc ctggaaaccc cgtaccgggc cgttctccac
     3301 ggccatccgc cgttttgtgt aatcctctcc ctggactgcc gggaatacga ctgcccccaa
     3361 cggggtggtt gccaccgctt ttgtcctcgg gagcgccgga tcgccggctt tgccgtcgtc
     3421 accggtgaag ccggcggcgg gccctacggc ttgggccgca ccgttccgcc tgccgtccgg
     3481 gagaccggcg ccgcaatcgt ctatggtcac ggcgtcttca ccgcggggcg ggatgatttt
     3541 cgggatgcat tccgccttct aaaagaattg gaaacaacct gccgaaggct tttcttccgg
     3601 atgatagaat tttaggtttc gttcagaaga tccccgcctg gcagcccccg gcgaggacac
     3661 cgcccagttc gcggcactgg gccaggatct ccggcgtgac ctgtccccgg gcgatgacgg
     3721 gattatagac cggcttgaat ttgtagccca gggcaatacg ctcgatggcc ttgagtgccc
     3781 ccgtaccgtc atttccggcg ctgatgaaca ccacataggg cttgcggaag accttgtcct
     3841 gggcgggata ataggttcgg tcaaagaaat cctttatcat tcccgacatg tacccaaagt
     3901 tctcgggggt gcccaccgcc agaccgtcgg cggtcaggag atcctccagg gtggcatcag
     3961 cggcccgttt gca
//
LOCUS       DAOSMF010000164         3958 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_24748_length_3958_cov_24.035101, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000164 DAOSMF010000000
VERSION     DAOSMF010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3958
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24748_length_3958_cov_24.035101"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="PLT63_10410"
     CDS             <1..672
                     /locus_tag="PLT63_10410"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HOD99174.1"
                     /translation="AYGWEWLHPLYGAVFAALLAAVIIGLVSMGARQREDTVIGALWA
                     IGMAVGILFISRTPGYNEDLMGYLFGNILMVSPGELWMIAGLDIVVVAAGTLFYNHFL
                     ALCFDEEFARLRGVRVEIYYLGLLCLTALTVVLLVSVVGIVMVIALLTLPAAVAGELT
                     KRLWHMMALATVLTVLFTTAGLAFSYGPDLPAGAVTVVFAGLAYLLAASGLQIIRRHG
                     RAKQP"
     gene            complement(720..1415)
                     /locus_tag="PLT63_10415"
     CDS             complement(720..1415)
                     /locus_tag="PLT63_10415"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOD99175.1"
                     /translation="MYRKLSALIVLLVLLPVQAAGLTCGGPGTQQAPTAYAAAGGEKL
                     AACFDLKAKNVALQLPDGRIVTLPAAVSASGARYSNGRETFWEHQGTGRYFVGDRLVF
                     EGRPATEAAYDTGVTSRVLTQTSVTGNGRQIVYPAAGQAEVTAMSVDIAPGAETGWHK
                     HPIPVYAYVLAGELAVELEGGRKLPFKTGDAIIEVVDTFHNGRNTGKEKVRLVVFYLG
                     VKGTPNVIKKATP"
     gene            complement(1462..2517)
                     /gene="mnmA"
                     /locus_tag="PLT63_10420"
     CDS             complement(1462..2517)
                     /gene="mnmA"
                     /locus_tag="PLT63_10420"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOD99176.1"
                     /translation="MNNKVAIAISGGVDSSVAAWLLREAGYEVCGVTMRLGIPAAGEE
                     AAPGGPPTGVAAKTVCEILDIPLYTLDVAADLEGLVIQPFLSAYLQGRTPNPCVECNR
                     SLKFGSLLRRVRDLGCDFLATGHYARVEYTSGGARLLKPRDLRKDQTYFLYAIDRKAL
                     GYVLFPLAPYRKEEVRELAAKAGLPGAERPESQDVCFLPDGDLASLLRRYGREEYWAG
                     DIVTRQGKILGRHRGLPFYTVGQRGGLGISHRVPLYVLALDVPGNRLIVGEKEEVRAA
                     GLRADSLNILMDAPPVRAAAKIRYAHRAAPCTVAWEKDGLTVRFDAPQEAVTPGQSVV
                     IYDGDLVAGGGIIREAL"
     gene            complement(2537..3022)
                     /locus_tag="PLT63_10425"
     CDS             complement(2537..3022)
                     /locus_tag="PLT63_10425"
                     /inference="COORDINATES: protein motif:HMM:NF002486.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HOD99177.1"
                     /translation="MDCPCGSGKTYDECCEPFIRGKAFPETAEQLMRSRYSAYTLQEI
                     PYITDTLHPDHREDWDEKGAGDWSRNSVWQGLEIVHTEGGGPRDEEGAVEFIASFTLK
                     DTALKHHERAVFERKDGRWYFKDGELVKPKQVRRETPKIGRNDPCPCGSGRKFKKCCG
                     A"
     gene            3371..3952
                     /locus_tag="PLT63_10430"
     CDS             3371..3952
                     /locus_tag="PLT63_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99178.1"
                     /translation="MKKRHWLWLLTVALIFAFVQTGMAAEAKKDAQADGIVLTETAQI
                     KAKVEDIDTAKRTVTLKGPRGNTVTLKADQSMENFDQIKKGDQVVATYIESMAVFVAK
                     AGEKPVTGEVEVVKAAPKGDKPSIIAVDTVQETLAVVGIDYKQRKITLKWPDGKTKTV
                     KIHKNVKGFKKIKKGDSIVVRLTEAVAVAVKTP"
BASE COUNT          725 a         1207 c         1215 g          811 t
ORIGIN      
        1 gcttatggct gggagtggct ccatcccctt tacggggccg ttttcgcggc gctgctggcc
       61 gcggtgatca tcggtctggt gagcatgggg gcccgccagc gggaagatac ggtcatcggg
      121 gccctctggg ccatcggcat ggccgtgggg atcctcttca tctcccggac ccccggctac
      181 aacgaagatc tgatgggtta tctcttcggg aacatcctga tggtctcccc tggagagctc
      241 tggatgatcg ccggcctcga cattgtggtc gtggcggccg ggacgctatt ctacaaccac
      301 ttcctggccc tgtgcttcga tgaggaattc gcccgcctcc ggggcgtccg ggtggagatc
      361 tactatcttg ggcttctctg cctcaccgcc ctgaccgtgg tcctcctggt ctccgtggta
      421 ggcatcgtca tggtcatcgc cctcctcacc ctgcccgcgg cggtggccgg ggaactgacg
      481 aaacgtttgt ggcacatgat ggccctggcc accgttttga ccgtcctatt caccaccgcc
      541 ggtctggcgt tcagctacgg gcctgatctg cccgccgggg ccgtgaccgt tgtttttgcc
      601 ggtctcgcct acctgctggc ggcttcaggg ttgcaaatca tccggcgcca tggcagggcg
      661 aaacagccgt aacccccgcc ttccagacgt cctccataaa tatatgaatc cattcgggat
      721 cagggcgttg ctttcttgat gacgttggga gttcccttca cccccaggta aaacaccacg
      781 aggcgtacct tctccttgcc ggtgttgcgg ccgttgtgga aggtgtcaac cacctcgatg
      841 atcgcatccc cggtcttgaa gggtagcttc cggccgccct ccaactccac cgccagctcg
      901 ccggccagga cataggcata aaccgggatc ggatgcttgt gccagcccgt ttcggcgccg
      961 ggggcgatat cgacgctcat ggccgtgact tcggcctggc cggcggccgg atagacgatc
     1021 tgccgcccat tgccggtcac gctggtttgg gtgagtaccc gggacgttac gccggtgtcg
     1081 tatgccgcct ccgtcgccgg ccggccctcg aacaccagcc tgtccccgac aaaataacgg
     1141 ccggttccct gatgttccca gaaggtttcc cggccgttgc tgtacctggc ccccgaagcc
     1201 gataccgccg ccggcagggt gacgatccgg ccgtcgggca gttggagggc gacgtttttc
     1261 gccttcaggt cgaagcaggc cgcgagcttt tctccccccg ccgcggcata ggcggtgggc
     1321 gcctgctggg ttcccggacc gccgcaggtc agaccggcgg cttgaaccgg cagcaatacc
     1381 agcaatacta taagggccga caatttcctg tacattttca agattcctcc ttcgtctatg
     1441 gaatatgcct tttgggtttc ttcacagcgc ttcccggatg atgccgccgc ccgcgacgag
     1501 atcgccgtcg tagatcacca cggattggcc cggggtgacg gcctcctgcg gggcatcgaa
     1561 acggacggtg aggccgtcct tttcccaggc gacggtgcaa ggcgccgccc ggtgggcgta
     1621 acggatcttc gccgccgccc ggaccggcgg cgcgtccatg aggatattga gggaatcggc
     1681 tctgagaccc gccgcccgga cttcctcctt ttccccgacg atcagacggt tgcccggaac
     1741 atccagggcc aggacgtaca gggggacccg atggctgatc cccaggccgc cccgctgtcc
     1801 cacggtgtag aaggggaggc cccggtgacg gcccaggatc tttccctggc gggtgacgat
     1861 gtcgccggcc cagtattctt ctcggccgta tcgccgcagc agagaagcaa ggtcgccgtc
     1921 gggaaggaaa cagacatcct ggctttccgg tctttccgcc ccgggcagac cggcctttgc
     1981 ggccagttcc cgcacctcct ccttgcggta cggggccagg gggaacagga catatccgag
     2041 ggctttccgg tcgatggcgt agagaaaata ggtctgatcc ttccggaggt cccgaggctt
     2101 cagaagtcgg gccccaccgg aggtatactc cacccgggcg taatggccgg tggcgaggaa
     2161 gtcgcagccc agatcccgga cccggcggag gagcgagccg aacttgaggg accggttgca
     2221 ctccacgcag ggattaggcg tccggccctg aaggtaggcg gagaggaagg gctggatcac
     2281 caaaccttcc agatcggcgg ccacgtccag ggtgtaaaga ggaatgtcaa gaatttcgca
     2341 aaccgttttc gcggccacgc ccgtcggggg gccgcccggg gcggcctctt cccccgccgc
     2401 cgggatgccc aggcgcatag tgacgccgca aacctcatac cccgcttccc tgaggagcca
     2461 ggcggccacg gaggagtcca cgccgccgct gatggcgatg gcgaccttgt tgttcatgat
     2521 ccggatttgc ggttctctat gccccgcagc attttttgaa tttcctgccg ctcccgcagg
     2581 gacaggggtc gttgcggccg atcttggggg tttccctccg gacctgtttc ggttttacca
     2641 actcgccgtc cttgaaatac cagcggccgt ccttcctttc aaaaaccgct cgttcgtggt
     2701 gcttaagagc ggtgtccttg agggtgaaac tggcgataaa ttcgactgct ccctcctcat
     2761 cccgagggcc gcccccttcg gtatgaacga tttccagtcc ctgccaaacg gagtttctcg
     2821 accagtcccc ggcccccttt tcatcccagt cctcccggtg atcggggtgc agagtgtccg
     2881 taatgtaggg tatctcctgc aaggtgtagg cgctgtagcg ggagcgcatc aactgctccg
     2941 ccgtttccgg aaacgccttt cccctgatga acggttcaca gcattcgtcg taagtttttc
     3001 ccgaaccgca tggacaatcc atatttcctt atcctcccgt gtagatgatc tcgaaaaatc
     3061 ggtatgttat gtaatttgct tctgccttga gaattgtttg tttcatcagg ggcaatgttc
     3121 gcgggaaaga tcgcggacgg gttttttcat acaccccgga acaggataat gcaaggtatt
     3181 atcaagagat tttctgaaag accccgccgc ttgagggcgg gagggtgtcg tcccgtaagg
     3241 aagggcggat catcattcct gtcccgggca ccgctggcga taatggcggt tgacatccgg
     3301 cctttcccgg ccttatactc ccgaggtccg caagggggcg gatcaccatt tagaatctga
     3361 ggaggctttg atgaaaaaga gacattggtt gtggttgctg acggtggcat tgatttttgc
     3421 gttcgttcag accggaatgg cggcggaggc gaagaaagac gcgcaggcgg atgggatcgt
     3481 tcttacggaa acggcccaga tcaaggcgaa ggtggaggat atcgacacgg cgaagcgcac
     3541 cgtgaccctc aagggacccc gaggcaacac cgtcaccctc aaggcggatc agtccatgga
     3601 aaacttcgac cagatcaaaa agggtgatca agtagtggcg acctatattg agtccatggc
     3661 cgtcttcgtc gccaaggcgg gcgaaaagcc ggtcacggga gaggtggagg tagtgaaggc
     3721 cgcccccaag ggagacaaac cgtccatcat tgccgtggat acggtccagg aaaccctggc
     3781 ggtcgtcggc atcgattaca agcaaaggaa gatcaccctg aagtggcccg acggcaagac
     3841 caaaacggtg aagattcaca aaaacgtcaa gggattcaag aagatcaaga agggtgattc
     3901 catcgtggtg cgcctgacgg aggccgtggc cgtggcggtg aaaacaccct gatcccgg
//
LOCUS       DAOSMF010000165         3942 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_24882_length_3942_cov_23.080010, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000165 DAOSMF010000000
VERSION     DAOSMF010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3942
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24882_length_3942_cov_23.080010"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(235..1677)
                     /locus_tag="PLT63_10435"
     CDS             complement(235..1677)
                     /locus_tag="PLT63_10435"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOD99179.1"
                     /translation="MPARFSQKTLSIARALIESVHDGMFIADHEGYFIMANEAFERIT
                     GINRQEMMDRHTQHMIDNNWVPVAVNLEVLKDYQTRSRIVRYPSGRQLLVTAAMLWRD
                     NGNPAAVVSTLRDLTELNRINEELKNSQVMIERFKKRIEYLEETLGGGANRFFVGQST
                     EFRRILSMAKKVARSDATVLITGPSGVGKDVLAQFIHYQSPRQNTGSFVKVDCASLPA
                     NLLESELFGYDKGAFTDARIQGKQGMFELANGGTLFLDEIGEFPFELQAKLLNVLQDR
                     QIKHLGGLQAIPVDVRIVAATNMNLEQMVRERKFREDLYYRLNVIPVFIPPLRERRED
                     ILPMIKHFLKVYNQNYNTGKNLSIDALNAMLNYDWPGNVREVRNTIERVVVMSADEIV
                     TRDDLPGEIRKALHLDAGVGTFPPRSAGAAAAPGPGAAASVPLRQALAVAEKDLIRRA
                     LESSATLAEAARRLQIDLSTLTRKKRKYNV"
     gene            complement(1750..3540)
                     /gene="ftsH"
                     /locus_tag="PLT63_10440"
     CDS             complement(1750..3540)
                     /gene="ftsH"
                     /locus_tag="PLT63_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOD99180.1"
                     /translation="MEKKHRFSLWYVLIGIWLVLIIQSYIAALFSVQVIPYSQFLTHL
                     KNGKITEIAVSANQIQGKMKVDEKSGELKAFRTIRVDQELSAMLTQYPVVFKGEIEST
                     FLRDLLSWVLPILLFIGIWYFIMKRFSSQQAGFLSLGKNKAKIYMEDELNIRFKDVAG
                     VDEAEQELVEVIDFLKNPKKFTDLGSKIPKGILLVGPPGTGKTLLAKAVAGESGVPFF
                     SMSGSEFVEMFVGMGAARVRDLFEQAKQKAPCIIFIDELDALGKARGFSPTGGHDERE
                     QTLNQLLVEMDGFDPKVGVILLAATNRPEILDPALLRAGRFDRHILVDRPDKKGREEI
                     LKVHLKKVKVGADIDVGVLAGMTPGMVGADLANLVNEAALLAVRHGKDVVEMKEFEEA
                     VERIIAGLEKKNRLINEEERKIVAYHEMGHALVALSLPGTDPVQKISIIPRGIAALGY
                     TMQVPTEDRFLMRKTELLNKVASLLGGRAAEQIVFGDISTGAHNDLSRATDIAKSMVK
                     EYGMSEKLGQVYLAHDQRQRFLDAGVRDGGDYSPETARDIDAEIKAIIDRQYAVALAI
                     LQEKRDALDRGAALLLEKEKIDGAEIKALL"
BASE COUNT          712 a         1133 c         1290 g          807 t
ORIGIN      
        1 ggcggcggcg gcgtttccat ggcgacggtc ctggagatga tctgatggcc ggccgccggg
       61 ccgcgctgat ggcgacaccg tcaccagcgg tcaaaaggcc ggccgggata atggttcgcg
      121 aacggtggag cggctaattc ccccgttccg agagtaaaac aagttaacgt cggtcctcct
      181 tttcaacccc ggaaaagccc cctcgcagtc agagggggct tttcattatt tccattagac
      241 gttgtacttc ctcttcttcc tggtgagggt ggagaggtcg atctggagac ggcgggcggc
      301 ctcggcgagg gtggcggatg attccagggc gcggcggatc aagtcctttt ccgcaacggc
      361 cagggcctgc ctgaggggaa cggaggcggc ggcgcctggg cctggggccg cggcagcgcc
      421 agcgctcctc ggcgggaacg tcccgacgcc ggcgtcgaga tgtaaagcct tgcggatctc
      481 cccgggcaga tcgtcacggg tgacgatttc gtcggcgctc atgacgacga cgcgctcgat
      541 ggtattgcgg acctcccgga cgttccccgg ccagtcgtag ttcagcatgg cgttcagggc
      601 gtcgatggag aggttcttgc cggtgttgta gttctggttg taaaccttga ggaagtgctt
      661 gatcatgggg aggatgtcct cccggcgttc ccggaggggc gggatgaaca cggggatgac
      721 gttcaggcgg tagtagaggt cctcccgaaa tttcctttcc cggaccatct gctcgaggtt
      781 catgttggtg gcggcgacga tgcggacgtc cacgggaatc gcctgcagcc cgcccagatg
      841 cttgatctgc cggtcctgga ggacattgag aagcttggcc tggagctcga agggaaattc
      901 gccgatctcg tcaagaaaga gggtgccgcc gttggcgagc tcgaacatcc cctgcttgcc
      961 ctgaatccgg gcgtcggtga aggccccttt gtcgtagccg aagagttccg attcgaggag
     1021 gttggcgggg agggaggcac agtccacctt cacgaaggag ccggtgttct gccgggggct
     1081 ctggtagtgg atgaactggg cgaggacgtc cttgcccacg ccggaagggc cggtgatcag
     1141 gacggtagca tcggagcggg ccaccttctt ggccatggaa aggatacgcc ggaattccgt
     1201 gctctggccg acgaagaagc ggttggcccc gcccccgagg gtctcctcca ggtactcgat
     1261 ccgcttcttg aagcgctcga tcatgacctg ggaattcttc agctcctcgt tgatccggtt
     1321 gagttccgtg agatcccgaa gggtgctgac gacggcggcg gggttgccgt tgtcccgcca
     1381 gagcatcgcc gccgtaacga ggagctgccg gccgctggga tagcggacga tgcggctccg
     1441 ggtctggtaa tccttgagca cttccaggtt caccgccacg gggacccagt tgttgtcgat
     1501 catgtgctgg gtgtggcggt ccatcatctc ctggcggttg atgccggtga tccgctcgaa
     1561 ggcctcgttg gccataatga agtagccttc gtgatcggcg atgaacatcc cgtcgtggac
     1621 cgactcgatg agggcccggg cgatgctcag ggttttctgg gaaaagcgtg ccggcatagc
     1681 aacaatatcc ttgtttcaaa aatccatcgt tcaaaaagga ccttcgaaaa aaagagaccc
     1741 gtctccattt tataggaggg ccttgatctc ggcgccgtcg atcttttcct tttcgagaag
     1801 gagggcggca ccgcggtcga gggcgtcccg cttttcctgg agtatggcca gggcgacggc
     1861 gtactggcgg tcgatgatgg ccttgatctc ggcgtcgatg tcccgggcgg tctcggggct
     1921 gtagtcgccg ccgtcccgga cgccggcgtc gaggaagcgc tggcgttggt cgtgggcgag
     1981 atagacctgg ccgagtttct cgctcattcc gtattccttg accatgctct tggcgatgtc
     2041 cgtggcccgg gagaggtcgt tgtgggcccc cgtggagatg tcgccgaaga caatctgctc
     2101 cgccgcccgc ccgccaagga gagaggcgac cttattgagg agctccgtct tccgcatgag
     2161 gaaccggtcc tccgtgggga cctgcatggt gtagcccagc gcggcgatcc cccgagggat
     2221 gatggagatc ttctgcaccg gatcggtgcc gggcagggag agggccacca gggcgtggcc
     2281 catctcgtgg taggccacga tcttccgctc ctcctcgttg atgaggcggt ttttcttttc
     2341 caggccggcg atgatgcgct ccacggcctc ctcgaactcc ttcatctcca cgacgtcctt
     2401 gccgtggcgg acggccagga gggccgcctc gttcacgaga ttggccagat ccgccccgac
     2461 catccccggc gtcatcccgg ccaggactcc cacgtcgata tcggcgccga ctttgacctt
     2521 tttgaggtgg accttgagga tctcctcgcg gcctttcttg tccgggcggt cgacgaggat
     2581 gtggcggtcg aagcgcccgg cccgcaggag ggcggggtcg aggatctcgg gacggttcgt
     2641 cgccgccagg aggatcaccc ccaccttggg gtcgaaaccg tccatctcca cgaggagctg
     2701 attgagggtc tgctcccgct cgtcgtggcc gccggtgggg ctgaagcccc gggccttccc
     2761 cagggcatcg agctcgtcga tgaagatgat gcagggcgcc ttctgcttgg cctgttcgaa
     2821 gagatcccgg acccgggccg cccccatgcc gacgaacatc tccacgaatt ccgagccgct
     2881 catggagaag aaggggacgc cgctctcccc ggccaccgcc ttggccagga gggtcttgcc
     2941 cgtccccggg gggccgacca ggaggatgcc cttggggatc ttgctgccca gatcggtaaa
     3001 tttcttggga ttcttcagga aatcgatgac ttcgacgagt tcctgctccg cctcgtccac
     3061 gccggcgacg tctttgaagc ggatgttcag ttcatcctcc atatagatct tggccttgtt
     3121 cttccccagg gagagaaagc ccgcctgctg ggaactgaag cgcttcatga tgaaatacca
     3181 gatgccgatg aacagcagaa tgggcaatac ccaggagagg aggtcccgga ggaaggtgga
     3241 ttcgatctcc cccttgaaga ccaccggata ctgggtcagc atcgcggaaa gctcctgatc
     3301 gacgcggatc gtccggaagg ccttgagttc ccccgatttt tcatcgacct tcatcttgcc
     3361 ctggatctga ttggccgaga cggcgatttc ggtgatcttg ccgtttttca ggtgggtgag
     3421 aaactgactg tacgggatga cctggacgga aaaaagggcg gcgatgtaac tctggatgat
     3481 gaggaccagc cagattccga tgaggacgta ccagagggaa aacctgtgtt ttttctccat
     3541 aaaacccctc ccaacgattt tgacgatccg ttagcggtct cccggcgccg ggccattgat
     3601 aattatcgaa attgccaggc taatttttgg ggctttctaa cagaaggcag gaaaaagctc
     3661 aaggagaaat acatctcaca aataaaacgg tctagtaaaa agtcgcaaaa tgggcacagg
     3721 catggcggtc cttggtcatg gagggcgccg cgcaccggcc actcggtggc ttacggaccc
     3781 cggcctcttt ccgtcttgac agaccgccgg gcaagcccta tataagcaag aaaaggggac
     3841 gcggatgggg aaatgggaag tgtcccccga ggaggattta tggaggcatt cccgcgcgtc
     3901 gcccggcgga tctatgagga ggcccggcag tcgcaacgtc tc
//
LOCUS       DAOSMF010000166         3942 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_24883_length_3942_cov_19.100592, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000166 DAOSMF010000000
VERSION     DAOSMF010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3942
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24883_length_3942_cov_19.100592"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            656..2458
                     /locus_tag="PLT63_10445"
     CDS             656..2458
                     /locus_tag="PLT63_10445"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOD99181.1"
                     /translation="MHIYQLDKPDNLVALLEESLAKYPDNLLFGTKNRQGVYEWITYR
                     EFGKRVDNLRGGLAQLEVTKGDAVGIIANNRVEWAVASFATYGLGGRFVPMYEAELLH
                     VWKYIVKDSGIKILFVSKPEIFEKVKDFTKDIPSLEKIFIIESTGDGSMAAVEKWGES
                     KPVASLRPQPDDIAGLIYTSGTTGDPKGVLLSHANLTSNSLAGIKKYPELNETARTLA
                     ILPWAHSYGQTAELHAIIRLGASMGLAESAATVAADLALVKPNWLVAVPRVFNRIYDG
                     LQKKMEETGGLAKTLFDMGVAAAQKRRELAAQGKSCFLTNLKFKIADKVVFAKVREKM
                     GGRLMGCMSGSAAMNPDIAQFFFDIGIPIYDCYGMTETSPAIAMNASYAYRLGTVGQA
                     IDKVKIVIDSSVVQEGATDGEIVVYGPNVMKGYHNKPEQTREVMTGDGGIRTGDRGRL
                     DQDGYLYITGRIKEQFKLENGKFVFPAAMEEDICLHPYVQNAVIYGENRPYTICLVVP
                     DFAAVEAYAEKNQLPKDMDALMEREEIRSLISNGITELLKKKYGGYEIPKKFVFLTEN
                     FTLENGLLTQTMKLKRRVVVDRFKEHIEAQYAKK"
     gene            complement(2780..>3942)
                     /gene="groEL"
                     /locus_tag="PLT63_10450"
     CDS             complement(2780..>3942)
                     /gene="groEL"
                     /locus_tag="PLT63_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005920153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOD99182.1"
                     /translation="KVGKEGVITIQDAKGMETTLEIVEGMQFDRGYISPYFVTDPNKM
                     EVRLEDPYLLLNEKKISSMKDLVPILEQVARTGKPLLIIAEDLEGEALATLVVNKLRG
                     TIKCAAVKAPGFGDRRKAMLEDIAVLTGGKVASEDIGVKLENLTLADLGQCKQLTIDK
                     DNTTIVDGAGKSADIQTRVKQIRAQMGETTSDYDREKLQERLAKIVGGVAIIKVGAAT
                     EIEMKEKKARVEDALNATRAAVEEGVVPGGGVALLRSLKALEKVSLPEDEQYGLNIIK
                     RALEEPLRQIATNAGFEGSIVMEKVKEGKNDYGFDADKGEYTNLMKAGIIDPTKVARL
                     ALQNASSVSSLLLTTEAMVAEAPKKKAPPMPPMPPGGMGGMGGMGGMDDYDY"
BASE COUNT          854 a         1089 c         1118 g          881 t
ORIGIN      
        1 gtatgttcca tgggcgtcat cgaggcaagc ctgaagacgg tcttcgcttc accttccaac
       61 gacgtctgtc ccctgctggc gcaacagccg gcgacaagca gggccgggat gacgaataaa
      121 gctataaatg ctaatcgttt aaataacata tgatgcctcc ttgctttgcg atccgctcat
      181 gacgaagata ttaaacgtaa gcccaaggct gggaaaagca atgtcgcctt cgggattaaa
      241 aatattccgt tgcgcattgt ccggataaag ccataacccg tagccaactc ccgcttgctt
      301 atccaagcaa atataagaca acctgtccgg gaaagtcaaa gattaattac cggagtgaat
      361 ctctttttga attgcagtct tgtctgacgg catcgtaaaa agtccggatg cttcgttgcg
      421 cttcatccct cgtcactgcg acgtacgtcc aagtacgcct cattcctcag gattcgcgcg
      481 ccttgcctgc ggatcttttt acgatgccgt cccaaatggg acattttttg actttttacg
      541 atgtcttctt gtctactgca tgcagtttga ggataaatat atttgaaata aaaaaactaa
      601 tcggataaaa tacagatgga agaaggaggg ggaattcttt ttccaaggag gcagtatgca
      661 catttatcaa ttggacaaac cggacaacct ggtggcgttg ctggaggaga gccttgccaa
      721 gtatcccgac aatctcctgt ttggcaccaa gaacaggcag ggcgtttacg agtggattac
      781 ctatcgggaa ttcggcaagc gggtggacaa cctccgcggc ggcttggcgc aactggaggt
      841 gaccaaaggt gacgcggtcg gaatcatagc caacaaccgc gtcgaatggg ccgtcgcctc
      901 ctttgccacc tacggactgg gggggcgctt cgtccccatg tacgaggcgg agcttctcca
      961 tgtctggaag tacatcgtca aggacagcgg gatcaagatc ctctttgttt ccaaaccgga
     1021 gattttcgaa aaagtcaaag attttaccaa ggatattcct tctttggaaa agatctttat
     1081 catcgagagc accggagacg gcagcatggc cgcggtggag aaatgggggg agtccaaacc
     1141 ggtggcgtca ctacgtcccc aaccggacga cattgccggg cttatctaca cctccgggac
     1201 gacgggggat ccgaaaggag ttctcctttc ccatgccaat ctgaccagca acagcctggc
     1261 gggcatcaag aaatatccgg aactgaatga gacggcgcgc accctggcca tcctgccctg
     1321 ggcccactct tacggacaga cggcggaact gcacgccatc atcaggctgg gggcctccat
     1381 ggggctggcc gaaagtgcgg ccaccgtggc cgcggatctg gccctggtga agccgaactg
     1441 gcttgtcgct gtcccccggg tcttcaaccg catctatgac ggcctccaga agaagatgga
     1501 ggaaacgggc gggctggcca agaccctctt cgacatgggg gtcgccgcgg cgcagaaacg
     1561 gcgggagctg gcggcgcaag gcaagtcctg tttcctcacc aatctgaaat tcaagatcgc
     1621 cgacaaggtg gtcttcgcga aggtccggga aaagatgggc gggcgcctta tgggctgcat
     1681 gagcggcagc gccgccatga accccgatat tgcccagttc ttcttcgaca taggtattcc
     1741 catttacgac tgttacggga tgacggaaac ctcgccggcc attgccatga acgcctctta
     1801 tgcctaccgg ctgggcaccg tgggccaggc tatcgacaag gtcaagatcg tcatcgattc
     1861 ctcggtggtt caagaagggg ccacggacgg cgagatcgtc gtttacggac ccaacgtgat
     1921 gaaggggtat cacaacaagc ctgagcagac ccgggaggtc atgaccggtg acggcgggat
     1981 tcgcaccggc gatcggggac gtctcgacca ggacggctat ctctatatca ccggccggat
     2041 caaggagcag ttcaagctgg aaaacggaaa atttgtcttt cccgccgcca tggaggagga
     2101 catctgcctc cacccctatg tgcagaacgc cgtgatctac ggagagaaca gaccgtacac
     2161 gatctgcctg gtggtacccg actttgccgc cgtagaggca tacgcggaga agaatcagct
     2221 acccaaggat atggatgccc tcatggaacg ggaggaaatc cgctccctga tcagcaacgg
     2281 gattacggaa cttctgaaga agaaatacgg tggttatgag atccccaaaa agttcgtctt
     2341 cctgacggag aatttcaccc tggaaaacgg ccttttgacc cagacgatga aactgaaacg
     2401 ccgggtagtc gttgacagat tcaaggaaca tatcgaggcc cagtacgcca aaaagtgaac
     2461 ggcgcggctg atgagcccgt tttggatctg ttccgtttgt ctcttgcctt acggggggaa
     2521 gttcggatgg atggggcatg agcttgtatg gcggcgtata aacccatccc cgccccggcc
     2581 ccccctgacg gggagggagc tgtagggatg aagagcgata ccgcccgtcc cggcggaaga
     2641 aaaaagcggc tggagatttt gatctccagc cgctgctgtt ttcggacatc ttttcgaatc
     2701 cgggccaaaa tatcgatgca tcttccggcc ggatattttg gccccggttc cgccgggtca
     2761 ggatatgatc ccgtcccgat caataatcgt aatcgtccat gccgcccata ccgcccatac
     2821 cacccatacc gccggggggc atcgggggca tcggcggggc cttcttcttg ggagcctcgg
     2881 cgaccatggc ctccgtcgtc aacagtagag aggatacgga ggaggcattc tggagggcca
     2941 gacgcgccac cttggtggga tcgatgatcc cggccttcat taggtttgta tattcgccct
     3001 tgtcggcatc gaatccgtag tcgttctttc cttccttgac cttttccatc acgatggagc
     3061 cttcgaaacc ggcattggtg gcgatctggc ggaggggctc ttcaagggct cgcttgatga
     3121 tgttgagtcc atactgctcg tcttcgggga gactgacctt ttccagggcc ttcagactcc
     3181 ggaggagggc gacgccaccc ccgggaacca cgccttcttc aacagcggcc cgcgtcgcgt
     3241 tcagggcgtc ttccacccga gccttctttt ccttcatttc tatttccgtg gccgccccca
     3301 ccttgatgat ggccacgccg cccacaatct tggcgagccg ttcctgaagc ttttcccgat
     3361 cgtagtcgga agtggtttcc cccatctggg cgcggatctg cttgacccgg gtctggatat
     3421 cggcgctctt gccggcgccg tccacaatgg tggtgttgtc cttgtcgatc gttaactgct
     3481 tgcactggcc gagatcggcg agggtaaggt tttccagctt tacgccgatg tcttcggatg
     3541 ccactttgcc gccggtgagg acggcgatat cttccagcat ggccttgcgt cggtcaccga
     3601 atccgggggc cttgactgcg gcgcatttga tggtgccccg gagcttgttg acaaccaggg
     3661 ttgccagggc ctctccttcg aggtcttcag cgatgatcag gaggggtttc cccgtccggg
     3721 cgacctgttc gagaatggga accaggtcct tcatggaact gatcttcttt tcgttgagga
     3781 gcaggtaggg atcttccaga cggacttcca tcttgttggg gtcagtgacg aaatagggag
     3841 atatgtagcc ccggtcgaac tgcatacctt ccacgatttc cagagtggtt tccatgcctt
     3901 tcgcatcctg aatggtgatg actccctcct tgcccacctt gt
//
LOCUS       DAOSMF010000167         3940 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_24899_length_3940_cov_21.075161, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000167 DAOSMF010000000
VERSION     DAOSMF010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3940
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24899_length_3940_cov_21.075161"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="PLT63_10455"
     CDS             <1..796
                     /locus_tag="PLT63_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99183.1"
                     /translation="KSEYAALANLDWSDTHRALQWLLEKKIFEQDERDMKRYRLNKYY
                     RDWLVQVGLRDLPDYKNHLSRMVKKHLALARASSHESGETPDPQPGKSPDAALSPIWR
                     NTRRPSGETPDGDLAKRQTKRGDSSLQSGGCDLLKKKERKGKENKDHEEVYSYFFCRD
                     LADLLEGHPFFSGLKSDADFWNALAAAYPDQDIPMQINRITAWLIANPNKRYKNYKRF
                     IQGWLSRADRKENDHGKTAPEQRRGDRDDSQERPAPFSDIPPELIA"
     gene            699..1637
                     /locus_tag="PLT63_10460"
     CDS             699..1637
                     /locus_tag="PLT63_10460"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD99184.1"
                     /translation="MEKQLPSNDAEIGTILKKGPLPFPISRRNSSPEPERRCSDCGAP
                     LHSIFMDHPSYLKVTGKEPHWTELPCEACERERFLREMEDRARDAVQQRLNTLLANSM
                     LRERFIDKTFENFLPFGKDREKQLRVLSIARDFAGAFHRHRQSGTWLLFMGSVGTGKS
                     HLCAAIINEVIRSGCTALFTKAPRLLREVKDTFHRDSEISQSEIIARMGEIDLLVIDE
                     VGIQFGTDTERMILYEILDLRYEAMRPVILTTNITDLKSLEKLLGERIIDRLFEGESK
                     IVVFEWESYRRFQKAAAGAMSPFKADAGIPVPGGRR"
     gene            1637..2518
                     /locus_tag="PLT63_10465"
     CDS             1637..2518
                     /locus_tag="PLT63_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99185.1"
                     /translation="MRHMTFSLPFLPPSLNVYRNDHWRKQRGEEKTWKDYIAVKWAEM
                     GRPKLKAVRIAMVFSFPDRRARDLDNYLATGSKLVGDAVKGFFIPDDSPEHLTAWSFR
                     FEFGGEAKTTVVIEEVDRNKDGGFSGEQATFWEDLPVPSKKTPAGEEVLSGRLSGRQT
                     MEVVSTPGRPACTLYETCNTPLCPLDGSSLNGIWYPDEEICRSRVHGNLTWMKAQRKL
                     ARVAAGGYFTVEMLNRLVVIRKGIAGLDPNLPEEPQLRAWFQKRAEKQDGATEAKAAR
                     TGHLKKYSFKRQVEGKG"
     gene            2638..2889
                     /locus_tag="PLT63_10470"
     CDS             2638..2889
                     /locus_tag="PLT63_10470"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOD99186.1"
                     /translation="MIEQDRKNQSPAGEKGERATTAAGPFAKRYYRVDEVAHYFSVSD
                     RTIYRLIDTGDIKAIRLRDCIRVSAEEIRELEEKFMISF"
     gene            2968..>3940
                     /locus_tag="PLT63_10475"
     CDS             2968..>3940
                     /locus_tag="PLT63_10475"
                     /inference="COORDINATES: protein motif:HMM:NF026010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99187.1"
                     /translation="MKGSIHFRKDRGYFYVSWVHKGKPYKIYKYNGEYLYHKKLAEKL
                     LACMQADEEGSFFRIEKYTREVPTDVIPYLKKWIELQEAHLSPATYKDYLNSVNNHLI
                     PWFKKHPLNVHEIRYDTLCQLLADIKREGKGKLNVIYCLRRCLDYAWKSGRILALPPF
                     PEKRLYGIEPKPIKWISEERQVKIIQAIPEEHQPIFWWLKYHLRRPSEAMALHKEDYD
                     QTTDSFIIRRAFSNKKLVEHTKTHKIHFIPCHSEFKPIMEKMPKTFGPYFFTNPTGRL
                     KGQHYQHDYLVDLWHAACAAVGEEIDMYAGLKHSSCSQYVNEKGLSID"
BASE COUNT          976 a         1090 c         1116 g          758 t
ORIGIN      
        1 caagagcgaa tatgcggccc tggccaacct ggactggtcg gacacccatc gcgccttgca
       61 gtggctgctg gagaaaaaga ttttcgaaca ggatgaaagg gacatgaagc gataccggct
      121 gaacaaatat taccgcgatt ggctggtcca ggtgggattg cgggatctcc cggactataa
      181 gaaccacctc agcaggatgg tcaagaagca cctggccctc gcccgtgcgt cgtcgcatga
      241 atctggtgaa acaccagatc cccaacctgg caaatcacca gatgcggcat tgtccccgat
      301 ctggcgaaac accagacgcc catctggtga aacgccagac ggcgatctgg caaaacgcca
      361 gacgaagcgc ggtgattctt ctttacaatc tggcggttgc gacctcctaa agaaaaagga
      421 aagaaaaggt aaagaaaata aagatcatga agaagtatat tcatacttct tctgtcgcga
      481 cctcgccgat ttgttggagg gtcatccctt tttttccggt ctgaaatcgg acgcggattt
      541 ctggaatgcc ctggccgccg cctaccccga ccaggacatc cccatgcaga tcaaccgcat
      601 aaccgcctgg ctgatcgcca atcccaataa acgatacaag aattacaaac ggttcattca
      661 aggctggctc tccagagccg accgaaagga gaacgatcat ggaaaaacag ctcccgagca
      721 acgacgcgga gatcgggacg attctcaaga aaggcccgct cccttttccg atatcccgcc
      781 ggaactcatc gcctgagccg gaaaggagat gctcggattg cggcgcgccg cttcattcca
      841 tcttcatgga tcatccctcc tacctgaagg tcaccgggaa ggagccccac tggacggagc
      901 ttccctgcga ggcctgcgag cgggaacggt ttctccgcga gatggaggat cgagccagag
      961 acgccgtcca gcagagattg aataccctgc ttgcgaacag catgctccgg gaacggttca
     1021 tcgacaagac cttcgagaat tttctcccgt tcgggaagga cagggaaaag cagctccgcg
     1081 tcctgtccat cgcgcgcgat tttgccggcg catttcatcg ccatcgccag tcggggacgt
     1141 ggcttctgtt catgggcagc gtaggcacgg gcaagagcca tctctgcgcc gctatcatca
     1201 acgaggtcat ccgctccggc tgcacggcgt tattcaccaa ggccccccgc ctcctcaggg
     1261 aggtcaagga caccttccac cgggattcgg agatttccca gagcgagatc attgcccgga
     1321 tgggcgagat cgacctcctg gtcatcgacg aggtgggcat ccagttcggc acggacacgg
     1381 aacggatgat cctctacgag atcctcgacc tgcgctacga ggccatgcgg cccgtgatcc
     1441 tcacgacgaa catcaccgac ctgaagagcc tggagaagct cctcggggaa cggatcatcg
     1501 accgcctctt cgagggggaa tcgaagatcg tcgtctttga atgggagagc taccggcggt
     1561 ttcaaaaagc cgcagcgggg gcaatgtcac cgttcaaggc ggatgccgga atccctgtgc
     1621 cgggagggcg gcgctgatga gacacatgac tttttccctg ccttttctgc caccgtcact
     1681 gaatgtctat cgaaatgacc attggcggaa acagagaggc gaggaaaaga cgtggaagga
     1741 ttatatcgcc gtgaagtggg cggagatggg caggccgaag ctgaaggccg tccggatcgc
     1801 catggttttc tccttcccgg accggcgggc ccgcgacctg gacaactacc tggccacggg
     1861 gagcaagctg gtgggggatg cagtcaaggg gttcttcatc cccgatgaca gcccggagca
     1921 cctgacggcc tggagcttcc ggttcgagtt cggaggagag gccaagacaa ccgtggtgat
     1981 cgaggaggtg gacaggaata aagatggagg cttttctgga gaacaggcga cgttctggga
     2041 agaccttccg gtgccgtcaa aaaaaacgcc tgccggggag gaggttttat caggacggct
     2101 ttcaggcagg caaacaatgg aggtcgtgtc cacccccggc cgcccggctt gcaccctcta
     2161 tgagacctgc aacaccccgc tgtgtcctct ggacggaagc agcctcaacg gcatctggta
     2221 tcccgacgag gagatctgcc gctcccgcgt ccatggaaac cttacctgga tgaaggcgca
     2281 gcgaaagctc gcccgggtcg ccgccggcgg ttacttcacc gtcgagatgc tgaaccgtct
     2341 ggttgttatt agaaagggaa tcgccggcct cgatccgaac cttccggaag agccgcagtt
     2401 gcgggcttgg ttccagaagc gggcggagaa gcaggatggt gcgactgagg cgaaggcagc
     2461 ccggaccggg catctgaaga aatacagttt caagagacag gtcgaaggga agggttaggg
     2521 gcaaatttta aaacggtcga gaaggcgggg tctaaccaaa atttagggtc gagacggcgc
     2581 gtcctacgac ctgaaacttt tagagagggg taaaccacgc acccggggtt tttgagagtg
     2641 atcgaacagg acagaaaaaa ccaatcacca gcgggagaaa aaggggaaag agcgaccacc
     2701 gctgccggcc ccttcgcgaa acggtactac cgggtcgatg aggtcgccca ctatttctcc
     2761 gtctccgatc ggacgatcta ccgtctcatc gacacaggcg acatcaaggc gatccgcctc
     2821 cgggactgca tccgggtttc cgcggaagag atccgggaac tggaagagaa attcatgatc
     2881 agcttttagg tgaaaccttt tggtaatatc atgttgaatc tttctgaaaa tatgcctata
     2941 catcgcccat ggggagggca tgtatgtatg aaaggttcaa ttcatttccg caaggatcgg
     3001 ggatactttt acgtatcctg ggtgcataag ggcaaaccct acaaaatcta caagtataac
     3061 ggcgaatacc tctaccataa aaagctggcc gaaaagctcc tggcctgcat gcaggcggac
     3121 gaagagggca gcttcttccg gatcgagaaa tacacccgcg aggttcccac cgacgttatc
     3181 ccctacctga agaagtggat cgagcttcag gaggcgcacc tttcccccgc cacgtacaag
     3241 gattacctga attccgtcaa caaccacctg attccgtggt tcaagaaaca ccccctcaat
     3301 gttcatgaaa tccgatacga caccctttgc caactcctgg ccgacatcaa acgggaaggg
     3361 aaagggaaac tcaatgtcat atattgtctg cgccggtgcc tcgattacgc ctggaaatcg
     3421 ggtcgtattc tcgccttacc gccgtttcct gaaaaacggc tctatgggat cgaacccaag
     3481 cccatcaagt ggatttccga agaacgtcag gtcaagatca ttcaggccat tccggaggag
     3541 catcagccga tcttctggtg gttgaagtat catctccgca gaccgtcaga agccatggcc
     3601 ctgcacaagg aggattacga ccagacgacg gacagcttta ttatccgcag ggcattttcc
     3661 aacaagaagc tggtggagca cacgaagact cacaagatcc atttcatccc ctgccacagt
     3721 gagttcaaac cgatcatgga gaagatgccc aagaccttcg gtccatactt ttttacgaat
     3781 ccaacgggtc ggttaaaggg gcagcactac cagcatgatt atcttgtgga tttgtggcac
     3841 gcggcgtgtg cggcggtggg tgaggagatc gacatgtatg ccggactgaa gcactcctcc
     3901 tgcagccagt acgtcaacga aaagggcctc agcatcgacg
//
LOCUS       DAOSMF010000168         3884 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_25370_length_3884_cov_18.669104, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000168 DAOSMF010000000
VERSION     DAOSMF010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3884
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25370_length_3884_cov_18.669104"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            201..1625
                     /locus_tag="PLT63_10480"
     CDS             201..1625
                     /locus_tag="PLT63_10480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOD99188.1"
                     /translation="MSYDPLEKKFSPDMERAKDKILAAGAEVPRGDVNFMLEAIIASS
                     PLAIIVADNTNYVRIWNPAAEQMFGWREEDVVGKLLPVVPDGKFTEFADIHAAVKRRD
                     AVIIKRTQRQRKDGEVLDVSLSVAPVLDGGNNIVGWVKIAADITEHLRGEIRLRESEE
                     KYRAILENMEEGFHEVNLRGNFTFFNKSFARIMGYSGDELMGMNYRHYAADEANRQAV
                     YAAYNSVFQTGEPLESFTWDIVRKDGMRRTVEVSTSLIRDMDGQPLGFRGIVRDITER
                     KASVDRLRKTLGATVQAIASLVETRDPYTAGHQRRVADLARAVATEMGLPRARIDGLS
                     MASTIHDIGKISIPAEILSMPRKLTDIEFGLIKSHPQAGFEILKDIDFPWPVARMVLE
                     HHERLDGSGYPRGLKDDDVLLESKILIVADVVESMASHRPYRPAHGLDAAMKEIAANR
                     GVLYDPDVVDACITIFRDKDFRMT"
     gene            complement(1666..3771)
                     /locus_tag="PLT63_10485"
     CDS             complement(1666..3771)
                     /locus_tag="PLT63_10485"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOD99189.1"
                     /translation="MFYSSDQPPKMEFLKLPLFASRRSALPFLAALIIFLAFFQAGTA
                     AAVEKVTLQLRWDHQFQFAGYYAALWQGYYRNAGLDVEIRSAITPDQMILKAVQEVAS
                     GRADFGIGAADILVARDHGHPLVLVATIFQKSAARFYALEKTRLDSPAALPRLRVARN
                     VNDLIDVELQAMLRAEGIDPGRVTPYPHEPGMAHLLNGRVDVIPGYETSIPYYAWRAG
                     VKVIGLSPSSYGINCYGDSLFTRRALAENRPEMISRFRAASLKGWRYALEHPREIADR
                     ITREFTRQDKIDDVRAFNRFQAEKLPGLILYPIVALGHTNPERWKKMHDLLRASGIVK
                     GELDPAEFIYDPEEQERYRQDTVRKTILWVLAVLGLAAAVSLLWILKLSRMIRQRDQA
                     LRERKASEERYRALFEYNPIETVVVDQQGRITAFNLTKRASGGGQPAIGQIMYRDYAR
                     EHATDMYKELMTTIHTGVSRSFPNTRYHDKYLDIRIAPFSAGAMITSIDMTARRRAED
                     RLRQAIGAIIRTLSMIVETRDPYTAGHQQRVADLARRIASELKMPLERIEGLRMAAIV
                     HDIGKISVPAEILSKPTSLTALEINFIRLHPQTGYEILKDMDFPWPIADMVRQHHERL
                     DGSGYPQGLKGEEISLEARILMVADVVEAMSSHRPYRPALGLEVALAEIETNRGILYD
                     VEAVKACLHLFREEGYRCE"
BASE COUNT          816 a         1043 c         1210 g          815 t
ORIGIN      
        1 agatcctggc ggacgtcccc atcggccgtc tgatcgaacc ggaagaggtg gcctccctgg
       61 tcggcgagct ctaccgtaac gaggccctgg ccggcgacgt ctttttcatt cacggcggcc
      121 tgcggctcgg ctcccgagga taggttgggc tgctgaccac cacgatcaag ttgtcatttg
      181 cgtgggaaag gatatttacc atgtcatatg atcccctgga aaagaagttc tcgccggaca
      241 tggaaagggc aaaggataag atacttgccg cgggcgcaga agtgccccgg ggcgacgtaa
      301 atttcatgct ggaagcgatt attgcctctt cgccattggc cataattgtt gcggacaaca
      361 ctaattatgt ccggatctgg aatccggccg cggaacagat gttcggatgg cgggaggaag
      421 atgtggtcgg aaagctgctg ccggtggttc ctgacggtaa attcaccgaa ttcgcggata
      481 tccatgccgc cgtgaagcgg cgtgatgcgg tcataatcaa aaggacacag cggcaaagaa
      541 aggatggcga ggtcctcgat gtgagccttt ccgtagctcc tgttttggat gggggtaata
      601 acatcgtggg ctgggtgaag atcgccgctg atattaccga gcatctacgg ggtgagatta
      661 gactccggga gagtgaggaa aaataccggg cgatcctgga aaacatggaa gagggattcc
      721 atgaggtgaa tctgcggggc aacttcacat tcttcaacaa atccttcgcg cggattatgg
      781 gatacagcgg tgatgaactg atgggtatga actaccggca ttacgctgcg gatgaggcaa
      841 accggcaggc ggtttatgca gcctataaca gcgttttcca aaccggcgag cctttggaga
      901 gttttacctg ggacattgtc cggaaagacg gaatgcgaag gacggtggag gtctctactt
      961 ccctgatcag ggatatggat gggcagccgc tggggtttcg gggtatcgtg cgggatatca
     1021 ccgaacggaa ggcgagcgtc gatcgtttac ggaaaaccct gggggcgacg gtgcaggcca
     1081 ttgcgtccct tgtagaaacc cgagatcctt atacggcagg ccatcagcgc cgggttgcgg
     1141 atctggcccg agccgttgcc acggagatgg gccttccccg tgctcggatc gacggcctca
     1201 gcatggccag taccattcat gatatcggta agatctccat tcccgcggaa atcctcagca
     1261 tgcctaggaa actgacggac atcgaatttg gcctgatcaa gtcacacccg caagcaggtt
     1321 ttgaaatatt gaaggatatc gactttccct ggcccgtggc gagaatggtc ctggaacatc
     1381 atgaacgcct ggacgggtcc ggttatcccc gcggtttgaa agatgatgat gtgctcttgg
     1441 agtcaaagat cctgatcgtt gccgatgtcg tggaatccat ggcctctcat cgtccctacc
     1501 ggccggccca cggcttagac gcggcgatga aggaaatcgc cgccaaccgc ggggtacttt
     1561 atgatcccga cgtggtggat gcctgcataa ccattttcag agacaaggac ttccggatga
     1621 catagaccgc ggatcaggtt ttgtcagtga taagaccggc cgcaattatt cgcagcggta
     1681 gccctcttca cggaagaggt gcagacaggc ttttaccgcc tccacatcgt atagaatgcc
     1741 gcggttggtt tctatttcgg ccagtgccac ctccaggccc agggcgggtc ggtaaggacg
     1801 gtgggaactc atggcctcca ccacgtccgc gaccatgaga atccgggcct ccaaggagat
     1861 ttcctccccc tttagaccct ggggataacc agagccatcc agacgttcgt gatgctgccg
     1921 gaccatgtcg gcgatcggcc agggaaaatc catgtccttc agtatctcat aacccgtctg
     1981 ggggtgaagg cgaatgaagt tgatttccag ggccgtaagt gaggtcggct tgctgaggat
     2041 ctccgccgga accgagatct tgccgatgtc gtggacgatc gccgccatcc tgagcccttc
     2101 gatccgttcc agcggcattt tcagctcgga ggcgatccgc cgggccagat cggccacccg
     2161 ttgctggtga cccgccgtat aggggtctct ggtttccacg atcatggaca gggtccggat
     2221 gatcgcccct atggcttggc gcagacgatc ctccgcccgg cgccgggccg tcatgtcgat
     2281 ggaggtgatc atcgccccgg cggagaaggg ggcgatccgg atgtccagat atttatcatg
     2341 gtaacgtgta ttcgggaagg agcggctgac accggtgtga atagtggtca tcagttcctt
     2401 atacatatcc gtggcatgct cccgggcgta atcccggtac atgatctgac cgatggccgg
     2461 ctgcccgccg ccggaggcgc gtttggtcag gttgaaagcg gtgatgcggc cttgttgatc
     2521 cacgaccacc gtttcgatgg ggttgtattc aaaaagggcc cggtaacgct cctcgctggc
     2581 ctttctttcc cggagggcct gatcgcgctg ccggatcatc cgggaaagct tgaggatcca
     2641 cagcagggag acggccgcgg ccagccccag gacggcgagt acccagagga tggttttgcg
     2701 caccgtatcc tgacggtacc gctcctgttc ctcggggtcg tagatgaatt ccgccgggtc
     2761 cagctctcct ttcacgatgc cgcttgcgcg gaggaggtcg tgcatctttt tccaccgctc
     2821 cggattggtg tgacccagcg ctacgatcgg atagaggatc aacccgggta acttttccgc
     2881 ctggaaacga ttgaaggccc ggacatcgtc gatcttgtcc tgacgggtaa attcccgggt
     2941 gatccggtcg gcgatctccc ggggatgctc cagggcatag cgccaaccct tgaggcttgc
     3001 cgcccgaaag cgggagatca tttccggtct gttttcggcc agggctcggc gggtgaagag
     3061 ggaatctcca taacagttga tgccgtaact tgacggcgag aggccgatca ccttcacgcc
     3121 ggcccgccag gcataatagg ggatggaggt ttcatagccg gggattacgt ctacccgtcc
     3181 attgaggagg tgggccatgc ccggttcatg gggatagggc gtaacccggc cgggatcgat
     3241 gccctcggcg cggagcatgg cctgtaattc cacatcgatc aggtcattga cgttccgggc
     3301 cacccggagg cgggggagcg ccgccggcga atcgagcctg gttttctcca gggcataaaa
     3361 ccgggcggcg cttttctgaa atatcgtggc caccaagacc aggggatgcc cgtggtcgcg
     3421 ggccacgagg atgtccgcgg cgccgatgcc gaagtcggcc cgtccggagg ctacctcctg
     3481 gaccgccttg aggatcatct gatccggggt aatggcggat cggatctcaa cgtccaggcc
     3541 ggcattccgg tagtacccct gccagagggc ggcgtaatag ccggcaaact gaaactgatg
     3601 gtcccaccgg agctgaaggg tcaccttttc aacggcggcc gccgttccgg cctgaaaaaa
     3661 ggccaggaag atgataaggg cggcgaggaa gggaagggcg ctccggcggg aggcgaaaag
     3721 aggtaatttc agaaactcca tcttgggtgg ttgatcgctg ctataaaaca atttccttgt
     3781 cccaataaaa ttggtggcgc accataagca tatttcccgc atttgtaaag aataaaaaga
     3841 gtccggggcg aataaaggat caatttcgac cgctcatgtc cgtg
//
LOCUS       DAOSMF010000169         3880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_25407_length_3880_cov_18.466405, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000169 DAOSMF010000000
VERSION     DAOSMF010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3880
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25407_length_3880_cov_18.466405"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="PLT63_10490"
     CDS             <1..826
                     /locus_tag="PLT63_10490"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HOD99190.1"
                     /translation="SWLILPLIVFTSAIAAPPPDAGWKRIDDGLHYGIFEVSAPHVVG
                     KAKILVVRIDPGLYEFSLLAASELRPLAGSLTVRQWADKYRLLAAINAGMFQKDMKTN
                     VGYMKNYAHINNPRLHRKYASVFAFNPVTAGGHPMIWDTDAKQLKQIISGYHTVIQNL
                     RLIKRPGLNRWSPQEKRWSEAALGQDEEGNVLFIFSKSPLSMHDLGNILLGLPIGIAC
                     AQHLEGGSQASLYLRHRGVVIKEAGASDLVMAVNGDEDGFLPIPNVIGIKKRSSKP"
     gene            841..3720
                     /locus_tag="PLT63_10495"
     CDS             841..3720
                     /locus_tag="PLT63_10495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOD99191.1"
                     /translation="MVSHPSIATKEEEIFRSVVENMLDATLIMDWDGTILFANDTALR
                     LMEMTSSEQMIGQNAAVFVQPESLSRVVSDLEAVRSGTTGFVSQYLLKTAQGNDLWVE
                     GLGTMITLGSRVLDLVILRDINIRRRLEAELREAYAEQERRIDERTAALKKLNSELRT
                     EIDERLRIEAALRESEEKFRKCAETAPVGIFIYQGSRYRYLNPACAKITGYSQDELFA
                     MDIWEIARPDLRGAVRNRIELRQRENFSSERYELVIITKGGEERWLDTSVTVMEYQGR
                     PAGLAVLVDITEQKRAAAALKRGEERYRSFLNTLKDMIFITDRKGRLIYMNPALEQRL
                     GYSESIVGKLFTEIVVPEYREDTVARYHQGMAGEGVPIYEIEFMPADGAGIPVELNVT
                     TYQDEEGHPAGRIGVARDISERREEEKLYQIMARTSSAGVYIVQDGRFQFINPNAAAY
                     AGYRAEEMIGMESQSILHPDDREEATRNAVRMLRGESTHPYSFRIRTKDGRSRWIMET
                     VATIRYRDRRAILGNSMDVTERMEMEKRLAESEVRYRSLFEGASDGILILEEEKFIVC
                     NRKAREIFQCREEDLLGRSPARFSTPRQPDGGDSEQQAHERLEFAGRGIPQFFEWRHC
                     RKDGKPFFAEVNLIRLDIPGKNLIQSIVRDISDRKAAEAALQASEERYRMIVENIEEG
                     YYETDLRGNISFVNDACQRIIGVGRQELLGNNFRVFTSAKDAKKLFHHFKNIFKTGQS
                     LRDLVWQVERPDGREQHLEVSASLIRDAAGRPSGFRGLIRDVTERRRAEEKVQWIAYH
                     DHLTGLPNRLLFQDRFTQLQAQAKRKDELFAVAVMDLNRFKEVNDQFGHAMGDMLLCG
                     VSARLSHHLREGDTVARMGGDEFLLLLPGIRERRDAAPLGEKIMQIFQQPFEASGQRF
                     TLTASVGMAVFPMDGVDYEVLIRKADQAMYRAKSEGRGVIVSNDP"
BASE COUNT          859 a         1178 c         1154 g          689 t
ORIGIN      
        1 gtcctggcta atattgccgt tgatcgtttt cacgtcggca atcgccgccc cgccgcccga
       61 cgcggggtgg aaaagaattg acgacggtct gcattacggg atcttcgagg tttcggcccc
      121 ccatgtcgtt ggcaaggcaa agatcctcgt cgtccgcatc gatcccggac tttacgaatt
      181 ctccctcctt gccgcttccg agttgcgtcc cctggcggga tccctgaccg tgcgacaatg
      241 ggccgacaaa tacaggttgc tcgccgccat caatgccggg atgttccaga aggatatgaa
      301 gacgaacgtg ggctacatga aaaactacgc ccacatcaac aacccccggc tgcacaggaa
      361 atatgcctcg gtcttcgcct tcaatcccgt aacggccggc ggccacccca tgatctggga
      421 caccgacgcg aagcaactca agcagatcat cagcggatac cacacggtga tccagaatct
      481 ccgcctcatc aagcgaccgg ggctcaaccg ctggtccccc caggaaaaga gatggagcga
      541 agccgcctta ggtcaggacg aagagggcaa cgtgttgttt attttttcta agtctcccct
      601 gagcatgcac gatctgggaa acatcctcct ggggctgccg atcggcatcg cctgcgccca
      661 gcatctcgag ggcggttcgc aggcgtctct ctacctgcgc caccgggggg ttgtaatcaa
      721 agaggcaggc gcatccgacc tggtcatggc agtgaacggc gatgaagacg gcttccttcc
      781 cattcccaac gtaatcggca tcaagaaaag aagttcaaaa ccataacgag ggacgaactc
      841 atggtttcac atccgtcaat tgccaccaag gaagaggaga tattccggtc ggtggtggaa
      901 aacatgctcg acgccaccct gatcatggac tgggacggta cgatcctctt cgccaacgac
      961 accgccctcc ggctcatgga aatgacgtcg tctgagcaga tgatcggaca aaatgccgcc
     1021 gtgttcgtcc agcccgaatc cctgtctcgc gtcgtctccg acctggaagc ggtccgaagc
     1081 ggcacgaccg gctttgtttc acaatatctc ctgaagaccg cccagggcaa cgatctgtgg
     1141 gtggaggggc tgggcacgat gatcaccctg ggcagccggg ttctggacct ggtcatcctc
     1201 cgggatatca acatccggcg ccgcctggag gcggaactcc gggaggccta cgcggaacag
     1261 gaacgacgaa tcgacgaacg gaccgccgcc ctgaaaaagt taaacagcga gctgcggacg
     1321 gaaatcgacg aacggctgcg gatcgaggcg gcccttcggg aaagcgagga aaagttccgg
     1381 aaatgcgcgg aaacggcgcc cgtggggatc ttcatctacc agggctcgcg gtaccgctat
     1441 ctgaacccgg cctgcgcaaa gattaccggc tacagccagg acgaactctt cgccatggac
     1501 atctgggaaa tcgcccgccc cgacctccgg ggggccgtcc ggaacagaat cgaactgcgc
     1561 cagcgggaga atttctcctc ggaacgctat gagttggtca tcatcaccaa agggggcgag
     1621 gaacgctggc tcgacacctc cgtaacggtg atggaatacc agggcagacc cgcggggctc
     1681 gccgtcctcg ttgacatcac ggaacaaaaa cgagcggcgg cggcattgaa gagaggcgag
     1741 gaacgctacc gctccttcct gaacaccctg aaggacatga ttttcatcac tgacaggaag
     1801 ggacgcctca tctacatgaa tcccgccctg gaacaacgcc tgggttactc cgaaagcata
     1861 gtcggcaagc tcttcaccga aatcgtcgtc cccgaatatc gggaggatac tgtggcccgt
     1921 taccaccagg gcatggccgg ggaaggagtg cccatctacg aaatcgaatt catgccggcg
     1981 gatggcgccg gaatcccggt ggaactcaat gtcaccactt accaagacga ggaggggcat
     2041 cccgccgggc ggatcggcgt ggcccgggac atctccgaac ggcgggagga ggaaaaactc
     2101 tatcagatca tggcccggac ctcttccgcc ggcgtctata tcgtccagga cgggcggttt
     2161 cagttcatca accccaatgc ggcggcctat gccggttacc gggcggagga gatgatcggc
     2221 atggaaagcc aaagcatcct tcatcccgac gaccgggaag aggccaccag gaatgccgtc
     2281 cgcatgctgc gcggagaatc gacccatccc tacagcttcc ggatccggac gaaagacggg
     2341 cggagccgct ggatcatgga aaccgtggcc accatccggt accgcgaccg gcgggccatc
     2401 ctcggcaatt ccatggacgt gacggagcgg atggagatgg agaagcgcct cgcggaaagc
     2461 gaagtccgct accgctccct cttcgaaggc gccagcgacg gcatcctgat cctggaggag
     2521 gagaaattta tcgtctgcaa ccggaaggcc cgggagattt tccaatgtcg ggaggaagac
     2581 ctgctcggcc ggagccccgc ccggttctcc accccccgcc agcccgacgg cggcgattcg
     2641 gaacagcagg cccatgagcg actcgaattc gccgggcgcg ggattccgca attctttgaa
     2701 tggcggcact gccggaagga cggtaagccg tttttcgccg aggtgaacct catccgtctc
     2761 gacatcccgg gcaaaaacct gatccagagc atcgtccgcg acatcagcga ccgcaaggcc
     2821 gccgaagcgg ccctccaggc cagtgaggaa cgctaccgga tgatcgtgga aaatatcgaa
     2881 gaaggttatt acgaaacgga tcttaggggg aacatctctt tcgtcaacga tgcctgccaa
     2941 cgcatcatcg gagtcggtcg ccaggagctg ctggggaaca atttccgggt gtttaccagc
     3001 gcaaaagacg ccaagaagct ttttcatcat ttcaagaata ttttcaagac cggccaatcc
     3061 ctgcgggatc tggtctggca ggtcgagcgc ccagacggcc gggaacagca tctggaagtc
     3121 tccgcttccc tgatccggga tgccgcgggc cgaccgtcgg gattccgggg actcatccgg
     3181 gatgtgacgg agcgccgccg ggcggaggaa aaggtgcagt ggatcgccta ccatgaccat
     3241 ctgacggggc tccccaaccg gcttctcttt caggatcgct tcacccagct tcaggcccag
     3301 gccaagcgga aagacgaatt gttcgccgtg gccgtcatgg atctgaaccg cttcaaggag
     3361 gtcaacgatc aattcggcca tgcgatggga gacatgcttt tgtgcggcgt cagcgcccgg
     3421 ctgtcccatc atctccggga gggggatacg gtggcccgca tgggcggaga cgaatttctc
     3481 cttctcctgc cgggcatccg ggaacgtcgt gacgcggcgc ctctagggga aaagatcatg
     3541 cagattttcc aacaaccctt cgaggcgtcg ggtcagaggt tcacactgac ggcgagtgtc
     3601 ggcatggcgg tttttcccat ggacggggtg gattacgagg tcttgatccg caaggccgat
     3661 caggccatgt accgggccaa atcggaaggc cggggggtaa tcgtcagcaa cgacccgtga
     3721 gccccgccgc aagcgacatt tcccgcctat gtcatcatct ttgcgcgggg gcttttatat
     3781 cctccccgtt caggaaggcg gccacggcgg ccctgatctt ctccccttcg cacataaagg
     3841 cgaaatggcc gcaaccgccg gtgagctcca acgactttcc
//
LOCUS       DAOSMF010000170         3810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_26059_length_3810_cov_21.936085, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000170 DAOSMF010000000
VERSION     DAOSMF010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26059_length_3810_cov_21.936085"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            318..674
                     /locus_tag="PLT63_10500"
     CDS             318..674
                     /locus_tag="PLT63_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99192.1"
                     /translation="MLNNRFQFLWKYPLTGKNSIPILQPTKSNDLSINNSNRRHLDDH
                     EPDDRGETYNVGGASEMENIAIVGMILDILDEIRPQDNGRPRWDLITHVKNCPGRDRR
                     PTRYAIDFSKLRQELG"
     gene            905..2227
                     /locus_tag="PLT63_10505"
     CDS             905..2227
                     /locus_tag="PLT63_10505"
                     /inference="COORDINATES: protein motif:HMM:NF013056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HOD99193.1"
                     /translation="MVRLTDYFDMRNSGADLPTEVIAGITTFLSAMYIIVVNPAILAE
                     AGIAPAASLTATVLVSAFSSILMGVYAKTPLVIAPGMSLNHMFVTLIVGAQGVPYETA
                     LGCVFYSGAIYLLLIVFDRRRLLISGVPRMLRYGFAGGIGLFIALIGMKASGLLVART
                     GGGLAIGDLTPAVISFLACFVITAGLVVRKIKGAFIWGVLISTIIALPIGRWWDDVPV
                     VIWQGWWAPPDFSLLGRLDLIGAAGMMHLPVIIVLVFSSFFDSYSTCVGVGEAGDLVD
                     EHGEPVAVRESLTCNAIAVALSGIVGASPATAYIESSTGVRAGGRTGLTAVVAGLLFL
                     PFLFLSPLVALVPPIATAPLLVLAGVFMLKPLIYVRWERFDEAVPFFVAMIIMPLSQS
                     ITQGAIWGLLSWTAIKAAGGRFRQISWIVIVLDLMAVFLLANMETFKH"
     gene            2258..>3810
                     /locus_tag="PLT63_10510"
     CDS             2258..>3810
                     /locus_tag="PLT63_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99194.1"
                     /translation="MTGKTGNKEIISAAGSQGVEAYIARYHELLEKPGRIVVATLEPV
                     HRRIDSSLHRSTDKGRVNLSAFVYAAERLPSCLPSVNRIVIASTEEVFEESGFGHVLD
                     WERAAARRRRRRSHYDGEQTLAIFVTSISDLDDLIPSLCAYQLEWNAIHRRLRSTPLG
                     ADLAAGRVRAMEAREDIRRALEVNSRDLEILLRFWSEESDHRFRDIAAAPKEMIVDRL
                     PLQQRDFEVSVTAWLDEVIRHFSDLDFVTRPVYLVSSNTHSLANLVSGYVRQRRDEII
                     AVALDEIIDDDDEWLRRYRQRLEDEEEEETLRNDFLYYALRAFLEKQPDRIPEKIAAE
                     ASAGVRRFTPDSLLHLEAQIIELEMLDPGSMDPGVSVGWGSPRPGATCPAPGRAPGMS
                     RKAGRDSDAESEIPIVFNIDYPLGFSAYYLMKTILGKMKRVQGVFILGKSAAIIGRLG
                     DIMIPEEVRDVHSGRNYRFRNVFSTARLVPYLSETAVFDDQRTLTVRGTFLHSRLMID
                     DYRGDDFTG"
BASE COUNT          761 a         1079 c         1107 g          863 t
ORIGIN      
        1 tacttacgtt tgtttattaa acaacaatta ccacttggaa tcaagctata ttaacatatt
       61 caagcccaat ttgccgtcca atacggcgca ttgattattg cttttgttaa ataccccacc
      121 ggaaatatcg aaaaatcgct ccaagaaacg tgtcaattta tccaggacgc cctcgcgttt
      181 ttttaccgct ctcccccagg agaaaagcat gacacgggcg gcaaaactcc ggccagctca
      241 gtccctgtgg ttgcgccgcc gccgctgcaa aacgcgttgc gcaccaattt ataggtttcg
      301 tcgtgatgga gattttcgtg cttaataatc gcttccagtt cctctggaaa tatcccttga
      361 caggcaaaaa cagcatccct atactccaac ccactaaaag caatgactta tcaatcaata
      421 atagtaatcg acgccatctg gacgatcatg aaccggatga ccgtggggag acctacaatg
      481 tgggcggggc ctcggagatg gagaatatcg ccatcgtcgg gatgatcctc gacatcctcg
      541 acgagatccg gcctcaggat aacggccgtc cccgctggga cctcatcacc cacgtcaaga
      601 actgccccgg ccgcgaccga cgaccgacgc gttacgcaat cgacttttcc aagctccggc
      661 aggaactggg ttagcagccc caagagtctt ttgccacggg cctgcggaaa accgtttcct
      721 ggtacgtgaa gaaccgggac tggacggacc gggtcccctc cggcgcctac cagcaatgga
      781 tcgacgagca gtatggagaa taagaggtaa gaggtgcaag tatgacgctc cgctgtgacg
      841 acggcgggat tcaagggaat tatcttcatc tccgtgctgc cttcacagga agtggattgc
      901 caagatggtg cgtctgaccg attatttcga catgagaaac agcggcgccg atttgcccac
      961 ggaggttatc gccggcatca ccaccttttt atcggcgatg tacatcatcg tggtaaatcc
     1021 ggcgatcctt gccgaggccg gcatcgctcc ggcggcatcc cttacggcga cggtcctcgt
     1081 ttcggccttt agttccatcc tcatgggggt ctatgcaaag accccgctgg tcatcgcacc
     1141 ggggatgagc ctcaaccaca tgtttgtaac gctgattgtg ggcgcccagg gggtgcctta
     1201 tgaaaccgcc ctgggatgcg tcttttactc cggggcgatc tatctgctgc tgatcgtctt
     1261 cgatcggcgc cgcctcctga taagcggcgt accccggatg ctgcgctacg gttttgccgg
     1321 tggcatcgga ctcttcattg ccttgatcgg gatgaaagca tcggggctgc tagtggcccg
     1381 aacgggagga ggcctggcca taggagactt gaccccggcg gtaatctcct tcctggcctg
     1441 tttcgtaatc accgccggat tggtcgtgcg caagataaag ggggccttca tctggggggt
     1501 tttaatttcc accattattg ccctgcccat cggccgctgg tgggacgacg tcccggtggt
     1561 gatctggcag ggttggtggg cgcccccgga cttcagcctg ttgggccgcc tcgacctgat
     1621 cggggcggcg gggatgatgc acctcccggt aataatcgtc ctcgttttca gctccttttt
     1681 cgacagctat tccacctgtg tcggcgtcgg cgaggcggga gatctcgtgg atgagcacgg
     1741 agagccggtg gccgtccgag agagcctgac gtgcaatgcc atcgccgtgg cgctgtcggg
     1801 aattgtcggc gccagcccgg cgacagccta tatcgagtct tccaccggcg tccgggcggg
     1861 cgggaggacc ggacttaccg ccgtcgtggc ggggctcctt ttcctccctt ttctcttcct
     1921 ctcccccctg gtcgcccttg tgcctcccat tgcaacggcg ccgctgcttg tgctggcggg
     1981 ggtttttatg ctcaaaccgc tgatttatgt acgctgggag cgtttcgatg aggcggtccc
     2041 cttcttcgtg gccatgatta tcatgcctct ttctcaatcc atcacccagg gggccatctg
     2101 ggggctgttg agctggacgg cgatcaaagc ggccggcggc cggtttcgac agatatcgtg
     2161 gatcgttatc gtcctggatc tcatggcggt ctttttgctt gcgaatatgg aaacgtttaa
     2221 acattagctg gcgtcgggcg ggtgtgcaca cggaaaaatg acgggaaaaa cagggaataa
     2281 agagatcatc tctgccgccg gatcgcaggg ggtggaggca tatatcgccc gctaccacga
     2341 acttctcgaa aaaccgggac ggatcgtagt ggcgactctt gagccggtac accgtcgaat
     2401 cgattcgagt ctccatcgct ctacggataa aggccgtgtg aatctttccg cctttgtcta
     2461 tgccgccgag cgtcttccct cttgcctgcc ttccgtcaac aggattgtca tcgcctccac
     2521 ggaagaggtc tttgaggaga gcggctttgg ccatgtcctg gactgggaaa gggccgccgc
     2581 ccgccgcagg agacggagat cccactatga cggcgagcag acccttgcca tctttgtgac
     2641 cagcatctct gatttagacg atctcatccc ctcactctgc gcctatcagc tcgaatggaa
     2701 cgccatacac cgccgcctcc gatccacgcc gctgggggcg gaccttgcgg ccggcagggt
     2761 acgggcgatg gaggcaagag aggatattcg ccgggccctg gaggtgaata gcagggattt
     2821 ggaaatcttg ctccgtttct ggtcggagga gagtgatcac cgttttcgcg acatcgccgc
     2881 ggcgcccaag gagatgatcg ttgatcgttt gccccttcag cagcgggatt ttgaggtatc
     2941 tgtaactgct tggttggacg aggtaattcg ccatttctcc gatctcgatt tcgtgacgcg
     3001 acccgtttac cttgtttcca gcaataccca cagtctggcc aatcttgtct cgggttatgt
     3061 gcgacagcgc cgggacgaga tcatcgccgt cgcgttagac gagatcatcg atgacgatga
     3121 tgaatggctg cgccgctacc ggcagcgact ggaagacgag gaagaggaag aaacgttgcg
     3181 caacgacttt ctgtattacg ccctgcgggc ctttcttgaa aagcaaccgg accgcattcc
     3241 ggaaaagatc gctgccgagg cttccgcggg agtgcgccgt tttacccccg atagccttct
     3301 tcatctggag gcgcagataa tcgaactgga aatgctcgat ccgggcagca tggatcccgg
     3361 cgtcagcgtg gggtggggat ccccgcggcc gggagcgacg tgtccggcgc cggggcgggc
     3421 gcccggaatg tcccgcaagg ccggaaggga cagtgacgcg gagagcgaga tacccattgt
     3481 cttcaacatc gattatccac tcggtttttc cgcttattac ctcatgaaaa cgatcttggg
     3541 gaaaatgaaa cgggtgcagg gggtttttat cctggggaag tccgccgcca taatcgggag
     3601 actgggggat atcatgattc ccgaggaggt gagagatgtc cattccggcc ggaattaccg
     3661 gttccggaat gtattttcca cggccaggct cgtgccctat ctgagcgaaa cggcggtctt
     3721 cgatgaccag cggaccctca cggttcgggg aacattcctc cattcccggc tgatgatcga
     3781 cgattatcgc ggcgatgact tcacgggcat
//
LOCUS       DAOSMF010000171         3808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_26082_length_3808_cov_27.672262, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000171 DAOSMF010000000
VERSION     DAOSMF010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3808
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26082_length_3808_cov_27.672262"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..353)
                     /gene="ssrA"
                     /locus_tag="PLT63_10515"
     tmRNA           complement(1..353)
                     /gene="ssrA"
                     /locus_tag="PLT63_10515"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(490..864)
                     /locus_tag="PLT63_10520"
     CDS             complement(490..864)
                     /locus_tag="PLT63_10520"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOD99195.1"
                     /translation="MSLITKIEKRKPGYYMVTLSGRLDGNTYADCEEEIRPLLVTDTQ
                     AIMFDMSELDYISSMGLRVILKTRKDIGSKGGSIHLINMQPQIEKVFEIVNLLKGMTL
                     FASLEEADAYFDAMQKKVLEAR"
     gene            complement(900..1361)
                     /gene="smpB"
                     /locus_tag="PLT63_10525"
     CDS             complement(900..1361)
                     /gene="smpB"
                     /locus_tag="PLT63_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003564285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOD99196.1"
                     /translation="MAAKESAAEKIIAQNKTARLHYFIDETYEAGIVLVGTEVKSLRD
                     GRANLKDSYALVREGEIFLHDLHISPYSFGNRYNHDPLRTRKLLLHSREIKRLYGKSR
                     ERGLTVVPLKLYFKRGKVKVEIGVGRGKKLYDKREAMKEKDARREMRRRDY"
     gene            complement(1437..2195)
                     /locus_tag="PLT63_10530"
     CDS             complement(1437..2195)
                     /locus_tag="PLT63_10530"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD99197.1"
                     /translation="MKNESVAVVKREHTKLHLHLKKWLEKAVLDYGMITAGDRVLVGV
                     SGGADSLVLLDLLASPMVFIPEFTLTAVHVNLGFAGGEKDAVHIEKEIKARVEDCVLE
                     KTDIGPVAHSEVNRKNPCFLCSRLRRRRIFEIAAARGCNRIAFAHHRDDIIETLLINM
                     FYGREISTMKPKQSIFKGKLHIIRPLAYLEEGLIKKYAGERGLAVVPNRCPTSGTSRR
                     LYVKELLRMLEKDNRQLRHNIWTAMHHVKADYLL"
     gene            2568..>3808
                     /gene="eno"
                     /locus_tag="PLT63_10535"
     CDS             2568..>3808
                     /gene="eno"
                     /locus_tag="PLT63_10535"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOD99198.1"
                     /translation="MTEIINVYAREILDSRGNPTVEAEVTIYSGITGRASVPSGASTG
                     EHEMLELRDGNKKRYLGKGVEKAVRNVIDKIGPEIIGMDCLDQRAIDYAMIALDGTEN
                     KSALGANAILGVSLACAKAAAAAVDLPLYRYIGGVGAKDIPVPMMNIINGGQHADNNV
                     DLQEFMIMPAGARNFREGLRMAAEVFHTLKTVLKGKGFNTAVGDEGGFAPNLKSNEEA
                     LIVIVEAIRKAKYKPGKDVFIALDPAASSFYKDGGYILVAEKKPKKTAAEMVAFYQDL
                     VDRYPIISIEDGLAEDDWEGWKLLTDALGKKIQIVGDDIFVTNRKRLEMGIKQGIANS
                     ILIKLNQIGTLTETLETIQMAKEAGYTTVVSHRSGETEDTAMADVAVAANCGQIKSGS
                     LSRSERLAKYNQLLRIEEELG"
BASE COUNT          860 a         1068 c          991 g          889 t
ORIGIN      
        1 tggtggaggc ggggggaatc gaacccccgt ccggaaatat tccactgcag gttcttcgtg
       61 cggagcctct gatttacttt cgccgccggc ccctcccaga ggcaagatgt accgtgacta
      121 tcctgtatga attcgcctcc ctcccctcag gcctgagagt tcggctatcc tgcctgtatt
      181 acgccctgtc cgacccggca ggcatgaccg gcagaacgtt agccgtctta cgcggctaag
      241 gcgtattcgt agtcgtttgc gactattttt ttcctaccta tttaacgagg cctgtaggaa
      301 cctcggcacg ctgctacagc ttcaactatc cccgtcgaaa ccgtgacgcc cccataacga
      361 ttatttcata ttatgagttt acaaaagttg ttggatgaca aagagcgatt acaaacctgc
      421 ttctatctta attaccggat cagtaaatgt caaggcaaag atggaatgtt cgggcgtggc
      481 ggtcgccaat catcgagcct ccaggacctt tttctgcatg gcgtcgaaat atgcgtccgc
      541 ttcctcgaga ctggcaaaaa gcgtcatccc cttcagcaaa ttgacgatct cgaacacttt
      601 ttcgatctgg ggctgcatgt tgatcaaatg gatgctgcct cccttgctcc cgatgtcttt
      661 ccttgttttc aggatgacgc gcaaacccat gctgcttatg taatccagct cagacatgtc
      721 aaacatgatg gcctgcgtgt ctgtgacgag aagcggccgg atttcttcct cgcaatccgc
      781 ataggtattg ccgtccaggc gaccgcttaa agtgaccatg tagtacccgg gcttccgctt
      841 ttcaatcttc gttatcagcg acatttcttc cctcctgtat acttggcgac aaagcctgat
      901 cagtagtccc gccttcgcat ctcccgccga gcgtcctttt ccttcatggc ctcccgcttg
      961 tcgtagagtt ttttgccccg gccgacgccg atttccacct ttacctttcc ccgtttgaag
     1021 taaagcttca ggggaacgac ggtcaagccc cgctcccggg atttgccata gaggcgtttg
     1081 atttcccggc tatgcagcag gagcttgcgg gtccgcaggg gatcgtgatt atagcggttc
     1141 ccaaaggaat acggactgat gtggaggtca tggagaaaga tttccccttc ccggaccagg
     1201 gcgtagctgt ccttgaggtt ggcccgtcca tcccgcaaag atttgacctc cgtacccacg
     1261 agcacgatgc ccgcttcata agtctcatcg atgaagtagt ggaggcgcgc cgtcttgttc
     1321 tgggcgatga tcttttccgc cgcgctttct ttggctgcca tagtctcttg accttagata
     1381 tcgaaacaaa tctaatgttt atattctccc ccacgccgac aaaccggaag ccctattcat
     1441 aatagatagt cggccttcac atggtgcatg gccgtccaga tgttgtggcg caactgacgg
     1501 ttatccttct ccaacatgcg gagcagttcc ttgacataaa gccgccgtga ggtaccgctg
     1561 gtggggcagc gattggggac cacggccaag ccccgttccc cggcatattt cttgatgagc
     1621 ccctcctcaa gataggccag ggggcggatg atgtgcagct tccccttgaa gatggactgt
     1681 ttgggcttca tcgtgcttat ctcccgcccg taaaacatat tgatcagcag ggtttcaatt
     1741 atatcgtccc ggtgatgggc gaaggcaata cggttgcacc cccgggcggc ggcaatctcg
     1801 aaaatccggc ggcggcggag acgggagcac aggaaacagg gatttttacg gttgacctcg
     1861 ctatgggcca ccggaccgat gtccgtcttt tccagcacgc agtcctcaac ccgggccttg
     1921 atttccttct cgatatggac tgcatccttc tctcccccgg cgaaaccaag attgacatgg
     1981 accgcagtaa gggtaaattc cgggatgaag accataggtg aggcaagaag gtccagaagg
     2041 accagactgt cggcaccgcc tgagactccg actagaaccc ggtctcccgc cgtgatcatc
     2101 ccataatcga gaacggcctt ctccagccac ttcttcaggt gtaggtgaag cttggtatgt
     2161 tctcgcttca ccactgccac actttcattc ttcaaagatc tccatgtcgg gtttgcccca
     2221 tcgcactatt ttcagtgaag ttgtttaatg tccataatgg gccgttgaaa tctagttcag
     2281 gcatattccc aataccggaa catatcaaaa accacttgcc atgcccctac ctatatccgg
     2341 ggtcccgtcg gaaacaatac acatcatcga ccgcatgacg ggcgatagct tcattccttt
     2401 ccggcatctg aacgtgggtg atgagcttat attagatcgc ccttgggatc aagacatgtt
     2461 ttgatctccc accatcataa ttacttgcaa attgtcggtc atttatgtca taagcaaccc
     2521 actaaagcaa cttcttctcc gtgaagaaga tcatcaggag gttctttatg acggaaatca
     2581 tcaatgttta tgcccgcgag attctggatt cccgtggcaa tcccactgtg gaggcggaag
     2641 tgaccatcta ttcgggaatc accggtcggg cctctgtacc ttccggcgcc tcgacagggg
     2701 aacatgagat gctggaactc cgggacggaa acaagaagcg ttacctggga aaaggggtcg
     2761 aaaaggcggt gcgcaacgtt atcgataaaa tcggaccgga aatcattggt atggactgtc
     2821 tcgaccagcg ggcaatcgac tatgccatga tcgccctgga cggcacggag aataaatcgg
     2881 ccttgggcgc gaacgccatt ctcggcgtct ctctggcgtg cgccaaagcc gcggcggctg
     2941 cggttgacct acccctttac cgctacatcg gcggtgttgg cgccaaggac atccccgtcc
     3001 ccatgatgaa catcatcaac ggcggtcagc atgctgataa caatgtggac ctccaggagt
     3061 tcatgatcat gcccgccggg gcgcggaatt tccgggaagg actccggatg gccgccgagg
     3121 tctttcacac cctgaaaacg gtcctgaagg gtaagggctt caacaccgct gtgggtgatg
     3181 aaggcggatt cgcaccgaat ttgaaatcca acgaagaagc cctcatagtc atcgtggagg
     3241 cgatccgcaa ggccaaatac aaacctggta aggatgtctt catcgccctc gatccggcag
     3301 cgagttcatt ttataaagac ggcggataca tcctggtggc ggagaaaaaa ccgaagaaga
     3361 cagcggcgga gatggtagcg ttctatcagg atctggtcga ccgctatccc atcatctcta
     3421 tcgaggacgg cctggcggaa gatgactggg agggatggaa gctgctgacg gatgccctgg
     3481 gaaaaaagat tcagatcgtc ggcgacgaca tcttcgtcac gaaccgcaag cgcctggaaa
     3541 tggggattaa gcagggcatt gccaattcca tcctcatcaa gctcaaccag atcggcaccc
     3601 tcacagagac cctggagacg attcagatgg ccaaggaggc aggctatacc accgtcgtct
     3661 cccatcgttc tggtgaaacg gaggacacgg ccatggccga cgtggccgtg gcggccaact
     3721 gcggccagat caagagcggc tccctttccc gcagcgaacg cctcgccaag tacaaccagt
     3781 tgctccgcat cgaggaagag ttgggaga
//
LOCUS       DAOSMF010000172         3799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_26176_length_3799_cov_20.658654, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000172 DAOSMF010000000
VERSION     DAOSMF010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26176_length_3799_cov_20.658654"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="PLT63_10540"
     CDS             complement(<1..185)
                     /locus_tag="PLT63_10540"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOD99199.1"
                     /translation="MKKILIADDNEANLYMLEILLKGEGFEVISAANGQEALEKALLT
                     PPALIVTDVLMPGMDGYA"
     gene            complement(262..1833)
                     /locus_tag="PLT63_10545"
     CDS             complement(262..1833)
                     /locus_tag="PLT63_10545"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD99200.1"
                     /translation="MRVGIYSNAPKIFVSESGKPAGIFIDILEYIAKIEGWTLRYVPG
                     TWKEGLEHLEAGDIDLMPDVAYTAAREKMYSFHKVPALSSWDQVYSGKDIRIQSLFDL
                     RGKRIVALEGSVQQTEFVRMVDSFDLKMTIMTVSNYQQAFEMVAQGGADAVVSNNFYG
                     KLNARRYGLDDSAVIFNPSALFFAAPKGRNDQLLHAIDIHLLEMKEDPQSIYYLTLKR
                     WTAEEVKFKLPVWMQVIGLVVGVVLLTSLVGSFVLKRQVNARTNELRLINQEMEQRII
                     ERTADLAKAMARAQEADRIKSAFLATMSHELRTPLNSIIGFTGLILRERVGPLNEEQK
                     KQLNMVRSSAQHLLALINDVLDISKIEAGQLTLVQEAVDLRKIIEKVAQSTLPLADSK
                     GLEMGIEIVPELSSIPGDARRIEQILLNLLSNAIKFTEKGSVKIVCTLDDAHVLVKVI
                     DTGIGIKYEDLGKVFETFRQIDSGISRKYEGTGLGLSISKRLAELMGGKLWVESVWEE
                     GSTFSLSLPKEGSHA"
     gene            2477..3145
                     /locus_tag="PLT63_10550"
     CDS             2477..3145
                     /locus_tag="PLT63_10550"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOD99201.1"
                     /translation="MELFTIPLGFDNSYLIRERQGALLIDAGTPGQAKTFGKALDEVG
                     VLPAEVKLIVITHGHWDHIGSARDIKEMTGAPLAMHQAECSWLETGKPPLPPGVTAWG
                     KIFRSILLAATPFIRIAPATVDIVLAEGYFSLVPYGIPGRIIPTPGHSPGSVSVLLDN
                     GEAFVGDLAMNQFPLCLRPGPPIFADDLGLVRRSWELLLERGAGMIYPAHGKPFPADL
                     LRQW"
     gene            complement(3176..>3799)
                     /locus_tag="PLT63_10555"
     CDS             complement(3176..>3799)
                     /locus_tag="PLT63_10555"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HOD99202.1"
                     /translation="KSAFHALNQKAAARRMAKDVGKRYEEIDIVVAHLGGGITVGTHR
                     RGKVIDCTHGLGEGPFTPERAGSLPTQDLLDLACSGKLDKKALQGRLVGQGGLVGYLG
                     TSDAQRVEEMIAAGDKRAELIYAALAYQVAKDIGAMATVLMGKLDGIVLTGGLAHSGR
                     LTSLIADRVRFLAPVHVHPGEDEMSALAEGGLRVLAGEEEVKAYGRP"
BASE COUNT          807 a         1155 c          982 g          855 t
ORIGIN      
        1 gcgtagccgt ccattcccgg catgagaaca tccgtgacaa tcagggctgg gggggtaaga
       61 agggcctttt ccaatgcctc ctgcccgttc gctgccgaaa taacctcgaa cccttccccc
      121 ttcaggagaa tttccagcat gtacagattg gcttcgttgt cgtcggcgat cagtatcttc
      181 ttcatgacat ctccttcaaa tccttcgcga gcaggcagtt ttcaacttgt tcttcacagg
      241 tgtcaatcag gattatccct ttcatgcgtg gctaccctcc ttaggcaagg acaagctgaa
      301 ggtgctgccc tcttcccaga cgctttccac ccagagcttg ccgcccatca actccgccag
      361 ccttttcgat atggaaagcc ccagccccgt cccctcatac tttctgctta ttccggaatc
      421 gatctgcctg aaggtttcga atacctttcc caaatcttca tatttgatgc cgattcccgt
      481 atcgataacc tttaccagga catgggcatc gtccaatgta cagacgattt tcaccgatcc
      541 cttttcggtg aatttgatgg cgttgctgag caggttcaga agaatctgct ctatccgtcg
      601 ggcgtctccc ggaattgaac tcagttcagg aactatctcg attcccattt caagcccctt
      661 gctatcggcg agcgggagag tgctctgggc aactttctcg ataatctttc gcaaatccac
      721 agcctcttgg accagcgtca actgtccggc ttcaattttt gatatgtcga ggacatcatt
      781 gatcagtgcc aggaggtgct gggcgctgct gcgcaccata ttcaactgtt ttttctgctc
      841 ctcatttaat ggtccgaccc gctcacgcag gatgaggccc gtgaaaccga taatggaatt
      901 caggggtgtc cgcaactcgt gggacatcgt cgccaggaag gcggatttga tccggtctgc
      961 ctcctgagcc ctggccattg cttttgccag atccgccgtg cgctcgatga ttcgctgctc
     1021 catctcctga ttgatcagcc gcagttcatt ggtgcgggca tttacctgtc gctttaaaac
     1081 aaagcttccc acgaggcttg taagcagaac gacacccacc acgaggccga tgacttgcat
     1141 ccatacaggc agtttgaact ttacctcttc cgctgtccag cgtttcaatg ttagataata
     1201 aatggattgc gggtcttctt tcatctccag caaatgaatg tcgatggcat gcaacaattg
     1261 atcgtttctg cctttgggcg cggcaaaaaa aagggcggac ggattaaaga taaccgccga
     1321 atcatcaaga ccatacctcc gggcattcaa cttcccgtag aaattattgg aaacaaccgc
     1381 gtcagcacca ccctgagcca ccatttcaaa tgcctgttgg taattcgaaa ccgtcatgat
     1441 ggtcatcttc aaatcgaagc tgtcaaccat gcggacgaat tccgtctgct gaacggaacc
     1501 ttcaagagcg acaattcgct ttccacgcaa atcaaataag gattgaatcc taatgtcttt
     1561 gccgctgtaa acttgatccc aagaggaaag agccggcacc ttgtgaaatg aatacatctt
     1621 ctcccgtgcc gccgtatagg caacgtccgg catcagatcg atgtcgccgg cctctaaatg
     1681 ttccaagcct tctttccagg ttcccgggac atagcgcaaa gtccatccct ctatcttcgc
     1741 aatgtactca agaatatcga tgaagatgcc ggcaggctta ccggattcgg aaacgaagat
     1801 cttcggtgca ttgctataaa tcccgacccg cacgacccga tcctctgcat ttgaacatgg
     1861 tattccggag cataaccctg tcagtagaat cagcaggaaa caccatgaga accggaacag
     1921 atgaatttta gtccctttgc tcttcctcac tatattgcct tattctagtg accgaaaatc
     1981 tgttatgtta attaggggat tatgacgggc agatagagac ccatcgccat tcaaacaacc
     2041 cggagataga cccgtcacgg ggacatatta attcatgagc ggtaggcctg tatgtctacc
     2101 tcactttttt tctctcatta atttcgcaac ttgtcaatgc aaatgagatt tatcccatac
     2161 tattaagtca ttgcgaaaca acccggcata aggtaagtta cggatcaagg agaccgccgc
     2221 gatgcccaac cgccggcaaa aggaggagca accgccggca ccgggagagg cgctgtcgcc
     2281 ggaccgggga gaacatcctc caccgccttg aagccgccgg ttcaacgacc cgccgagaat
     2341 cgccggcaga tcaccgggag gaaaagctgg cgccggagcg gagatgctgg gtcgcgcctt
     2401 ctcgaccccc attgcatcga ttccctcggc ccacgccatg ttcatgaccg cgatcgtcac
     2461 ataggaggac atgtttatgg agctattcac catcccgctg ggattcgata attcctatct
     2521 gatcagggaa aggcaggggg cgttgctcat cgacgccggc acgccgggcc aggcgaagac
     2581 cttcgggaag gccctggacg aagtcggcgt cctcccggcg gaggtgaagc tcatcgtcat
     2641 cacccacggc cactgggacc atatcggttc ggcgcgggac atcaaggaaa tgaccggcgc
     2701 ccccctggcc atgcaccagg ccgagtgttc ctggctggaa acggggaagc caccgctgcc
     2761 tcccggcgtg acagcctggg gaaagatctt ccggagcatc ctgctcgccg cgacgccctt
     2821 cattcgtatc gccccggcta ccgttgatat cgttctcgcg gagggatatt tctccctggt
     2881 tccctatggc atccccggcc ggataatccc cacccccggc cattcccccg gctccgtaag
     2941 cgtgctcctg gacaacggcg aggccttcgt gggcgatttg gccatgaacc aattccccct
     3001 ctgcctcagg cccggcccgc ccatcttcgc cgacgatctg ggcctggtga gaagaagctg
     3061 ggaactcctt ttggaacggg gggccgggat gatctacccg gcccacggca agccgtttcc
     3121 cgcggacctt ttgcggcagt ggtgatgcag gtgtaaggaa ctttaagttt cgcgttcaag
     3181 gccggccata ggccttgacc tcctcctccc ccgcgaggac ccgcaggcct ccctccgcca
     3241 gggcggacat ctcatcctcc ccgggatgga catgaacggg ggccagaaaa cggacccggt
     3301 cggcgatgag ggaggtcagg cgcccggaat gggcgaggcc gccggtgagg acgatgccat
     3361 ccagcttgcc catgaggacc gtcgccatgg cgccgatgtc cttggccacc tgataggcca
     3421 gggcggcata gatcagttcc gcccttttat cccccgcggc gatcatctcc tccacccgct
     3481 gggcgtccga ggtgcccagg tagcccacca gccccccttg acccaccagg cgaccctgga
     3541 gcgccttctt gtcgagcttg ccggagcagg cgaggtcgag gagatcctgg gtgggcaggg
     3601 aaccggcccg ctcgggggtg aaggggccct cgccgaggcc gtgggtgcag tcgatcacct
     3661 ttccccgccg gtgggtcccc acggtaatgc cgccacccag gtgggccacg acgatatcta
     3721 tttcctcgta tctcttcccg acgtctttcg ccatccgccg ggccgccgcc ttctggttca
     3781 gggcgtggaa ggcgctctt
//
LOCUS       DAOSMF010000173         3782 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_26326_length_3782_cov_24.761470, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000173 DAOSMF010000000
VERSION     DAOSMF010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3782
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26326_length_3782_cov_24.761470"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="PLT63_10560"
     CDS             <1..448
                     /locus_tag="PLT63_10560"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOD99203.1"
                     /translation="IQSNPWMFFLMANVCLLMGLSMLDAFALPIRTPRFIASLQPRKK
                     TRGAVGALLVGAASGLVMGPCTAPVLAVLLSFAATRQNVPYAMSLLFVFSLGMGTLLI
                     LLGTSAGVLANLPRSGMWMTRVSKIFGWLMIAAGEYFLLQAGMLWC"
     gene            512..991
                     /locus_tag="PLT63_10565"
     CDS             512..991
                     /locus_tag="PLT63_10565"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOD99204.1"
                     /translation="MTRLARYFFVAAVVAAAVLHPAADRCFAQERAPLFGIRDINGRI
                     FSMNDHGRKTVLLVFGTTWCSSCRSEIPYYKEIYHTYTPRGLVMVGIDIQEAQGKVAR
                     FADRHKLPYRVLLDETGAVAAAYRIVGVPTMVLVSNGVVVTRNYRQVNGSLQKLFPK"
     gene            complement(1065..>3782)
                     /locus_tag="PLT63_10570"
     CDS             complement(1065..>3782)
                     /locus_tag="PLT63_10570"
                     /inference="COORDINATES: protein motif:HMM:NF013179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOD99205.1"
                     /translation="ILFANQAAADLVQLESPAAGIGRNAIEFIHPDFIAVVAEDLLNV
                     REGGGGYLNRYQVITARGRERWVEGLGTRIPFGAGTANLVTIRDIGEVKESERIIRES
                     ETRLRELSVLQQAILDGANYSIIFIGSDGMIKTFNAAAERSLGYRAEELIDIHTPSLF
                     HDPGEVRRRAEELSRELGREVAPGFEVLVARTRLGIPDEREWTYIRKDGSRFPVSLSV
                     TALRDASGEIRGFLGIGSDITARLQAEEELRKLSQAVMQSPVAVIITTPDGRIDYVNP
                     RFTEITGYTAAEAVGENPRILKSGRMSEGEYRTLWETISAGRIWQGVFYNRKKDGGYY
                     WASASISPIRNPHGEVTHFVGIQEDITVQKMTEQALKEAKEAAEAANVAKSEFLAGMS
                     HEIRTPMNAVIGMAELLAETPLNQEQQQYLQIFRSAGENLLGLINDILDLSKVEAGRL
                     TLDASSFHLGEVVERTCEIMSMRAHEKNIELACRIAPDVCPELWGDAARLQQVLINLI
                     GNAVKFTEVGEVVVTVDQETPLSASLSEPDCLLRFSVRDTGIGIPQDKLDRIFERFTQ
                     ADSSTTKRYGGTGLGLTISRQLAELMGGRIWVESEVGKGSVFHFTARFQVRKYPEDAA
                     TAPEADISGLRVLVVDDNATNRLILKETLARMGGVVTECGSGPEALLLVREARRRGEH
                     FPLVLLDQRMPEMDGWEVAAAMRKEPEPAGTTILMLTSENRDVDVSRVAAMGLDDYLI
                     KPVKRADLVRAIARALKKRAVAAVTAAPAAPVLEEAPRPLRILLVDDSDDNRFLMAAY
                     FKNTPHEVEMAENGAQAVEKFQAGDYDLVFMDMQMPVMDGYTATRRIRAWEREQGRRP
                     TPVIALTAYALKDDMEKSLAAGCDAHLTKPIKKGKLLETVAAYAGRPAG"
BASE COUNT          649 a         1248 c         1138 g          747 t
ORIGIN      
        1 gatccagagc aatccctgga tgtttttcct catggccaat gtctgcctgc tcatgggctt
       61 gtccatgctg gacgcctttg ccctgcccat ccggacaccc cggttcatcg cctcgctgca
      121 gccccggaaa aagacccggg gggccgtcgg cgcgctgctg gtcggcgccg cctcgggact
      181 cgtcatggga ccctgcaccg ccccggtgct ggccgtcctg ctgagcttcg ccgccacccg
      241 acagaatgtt ccgtatgcca tgtcgctgct gtttgtcttc tccttaggca tgggcaccct
      301 cctgattctc ctcggcacct ccgcgggcgt cctggccaat ctgccccgtt cgggaatgtg
      361 gatgaccagg gtgagcaaaa tcttcggatg gttgatgatc gccgcggggg agtatttcct
      421 cctccaggcg gggatgctct ggtgctgact ccggggtcgt catgatggaa ggcaggggaa
      481 tacggaacta ctttcgatcc aaggagacaa gatgacaaga ctcgcgcgct atttttttgt
      541 ggccgccgta gttgccgcgg cggtcctgca tccggccgcc gatcggtgtt tcgcccagga
      601 acgggcgccg ctcttcggca tccgggacat caacggccgg atcttcagca tgaacgatca
      661 cggcaggaaa accgtcctcc tcgtcttcgg aaccacctgg tgttcctcgt gccgttccga
      721 aatcccctat tacaaggaga tataccacac ctacaccccc cgggggctcg tgatggtcgg
      781 catcgatatc caggaggcgc agggaaaggt cgcccgtttt gccgatcgcc acaagctgcc
      841 gtaccgggtc ctgctggatg aaacgggagc cgtggcggcg gcctaccgga tcgtcggggt
      901 ccccaccatg gtcctcgtca gcaacggggt ggtcgtcacc agaaattacc gccaggtgaa
      961 cggctcgctg caaaagctgt ttcccaaatg acttaaaacc gccgggccgc cgggggaaag
     1021 cgatcatcgg gacgacccgg ggactccacg ggagcccgcc gccgctaacc ggcggggcgg
     1081 ccggcatagg ccgcgacggt ttccaagagc ttgcccttct taatcggttt cgtcaggtgg
     1141 gcgtcgcagc ccgccgccag gcttttttcc atgtcatcct ttagggcgta agccgtcagg
     1201 gcgatgacgg gggtgggccg gcggccctgt tcccgttccc aggcccggat tctccgggtc
     1261 gcggtataac cgtccataac gggcatctgc atgtccatga acacgaggtc gtaatcgccc
     1321 gcctgaaatt tttccacggc ctgcgccccg ttttccgcca tctccacctc atggggggtg
     1381 ttcttgaagt aggccgccat gagaaagcgg ttgtcgtccg aatcgtccac caggaggatc
     1441 cggagcggcc gcggcgcctc ctcgaggacg ggcgccgccg gagccgctgt caccgccgcg
     1501 actgcccgtt tcttcagggc cctggcgatg gcccggacca ggtcggccct tttcaccggt
     1561 ttgatgagat aatcgtccag gcccatggcc gccacccgcg agacatccac atcgcggttc
     1621 tccgaggtca gcatgaggat ggtcgttccc gccggctccg gttccttccg catcgccgcc
     1681 gccacttccc agccgtccat ctccggcatg cgctgatcca ggaggaccaa cgggaaatgc
     1741 tccccgcgcc ggcgggcctc ccgcaccagc agcagggcct ccgggccgct cccgcattcg
     1801 gtgacgacac cccccatccg ggccagcgtc tccttgagga tcagccggtt ggtggcgttg
     1861 tcatcgacca cgaggacgcg cagaccggag atgtccgcct ccggtgccgt tgccgcgtct
     1921 tcgggatatt tccggacttg aaagcgggcg gtgaaatgaa agacgctccc tttgcccacc
     1981 tcgctttcca cccagatccg gccgcccata agttcggcga gctggcggga aatggtcaat
     2041 cccaggccgg tgccgccata gcgcttggtg gtggaagaat cggcctgggt gaaccgctcg
     2101 aaaatacgat ccagtttatc ctggggaatg ccgatgcccg tatcccggac ggaaaaacgg
     2161 aggaggcaat ccggctccga cagagaggcg gaaagcggcg tttcctgatc caccgttacc
     2221 accacttccc cgacctcggt aaattttacg gcgttgccga tgagattgat caggacctgc
     2281 tggagccggg cggcgtcccc ccacagctcc ggacagacat ccggggcgat ccggcaggca
     2341 agctcgatgt tcttctcatg ggcccgcatg gacatgatct cgcaggtcct ttccaccacc
     2401 tcgccgagat ggaaggaaga ggcatcgaga gtcagccgcc ccgcctccac cttggaaaga
     2461 tcaagtatat cattgatcaa accaagcaga ttctccccgg cggaacggaa gatctggagg
     2521 tactgctgct gttcctggtt cagaggcgtc tcggccagca gttccgccat cccgatcacg
     2581 gcgttcatgg gcgtcctgat ctcatgactc atcccggcca gaaactcgct tttggccacg
     2641 ttcgccgctt cggcggcttc tttggcctcc ttcagggcct gttccgtcat cttctggacg
     2701 gtgatgtcct cctggatgcc cacaaaatgg gtaacctcac cgtgaggatt gcggatcggt
     2761 gagatggacg ccgaagccca gtaatagccg ccgtcctttt tccggttgta gaagacgccc
     2821 tgccagatcc ggccggcgct gatagtctcc cagagggtcc ggtactcccc ttcggacatc
     2881 cgtcccgatt tcaggatccg gggattttcc cctacggcct ccgccgccgt atagccggta
     2941 atctccgtga accggggatt cacgtaatcg atcctgccgt cgggcgtggt aatgatcacc
     3001 gctacggggc tttgcatgac cgcctgggag agcttccgca gctcctcctc cgcctgcagg
     3061 cgggcggtga tgtcgctccc gatgcccaaa aatcccctga tctccccgga ggcgtcccgg
     3121 agtgccgtca cggaaaggga aacgggaaac cggctgccgt ccttgcggat gtaggtccat
     3181 tcccgttcat cggggatgcc caggcgggtt ctggccacca agacctcgaa gcccggcgcc
     3241 acttctctcc ccagctcccg ggacagttcc tccgcccgcc ggcggacttc gccgggatca
     3301 tggaagagcg acggggtgtg gatgtcgatc agctcttcgg cccggtagcc gagggaccgt
     3361 tcggcggcgg cgttgaaggt cttgatcatt ccgtccgaac cgatgaagat gatggaataa
     3421 tttgcgccgt ccaggatggc ctgctggagg acggacagtt cccgcagacg ggtctcgctt
     3481 tcccgaatga tccgctcgga ctccttgacc tcgccgatgt cccggatcgt gaccagattc
     3541 gccgttcccg ctccgaaggg aatccgcgtt cccaggccct cgacccagcg ctcccgtccc
     3601 cgggccgtga tcacctggta gcgattcagg tagccgcccc cgccctcccg gacgttcaac
     3661 agatcctcgg ccacgacggc gatgaaatcg ggatgaatga attctatggc gttgcggccg
     3721 atgcccgccg cggggctttc caactggacc agatcggccg ccgcctgatt ggcgaagaga
     3781 at
//
LOCUS       DAOSMF010000174         3772 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_26419_length_3772_cov_25.484262, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000174 DAOSMF010000000
VERSION     DAOSMF010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3772
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26419_length_3772_cov_25.484262"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /gene="era"
                     /locus_tag="PLT63_10575"
     CDS             complement(<1..725)
                     /gene="era"
                     /locus_tag="PLT63_10575"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOD99206.1"
                     /translation="MFKSGFIGIVGRPNVGKSTLLNSILEENIAITSPKPQTTRNRIM
                     GIKHQADGQMIFLDTPGIHDPHKPLNAAMVKTARNVAGDVDVLLVLIEAGEDIPPEDR
                     ALLATLQSYRQRAILAVNKMDLVKKDALLPLIDAARTIFPCRAFIPLCARTGDGVPVL
                     LQEIRSLLPAGPPYFPEDMLTDRPERFIAAEIIREKIISYTHKEIPYATAVVVDAFKE
                     EEGKELIRIEATINVERDSQKKI"
     gene            complement(740..1747)
                     /locus_tag="PLT63_10580"
     CDS             complement(740..1747)
                     /locus_tag="PLT63_10580"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOD99207.1"
                     /translation="MIIPFFLRNRGCPHRCRFCNQEISGGADPRKLGAGYFHEKVRAF
                     LTSAARGKKPGQPVEIAFYGGNFTGLTPEEQEDLLHMARRHMENGTVVGIRVSTRPDC
                     LRDKDVERLAAFGVRTVEIGAQSLVDGVLAASGRGHTARDVVAAVAMLKHRGLEVILH
                     LMAGLPGDDGGAFLDTVSRTTALGPQGVRIHPTLVLAGSPLAEDFLQGRYVPLTLPEA
                     VDLCRQAWDILENAGIAIIRLGLQATDILSGPGAVLGGPYHPAFGALVASARWRERAL
                     ALLGGADLEGRKAVFFVPPGEESAFRGHENCNIKFLRNHCRLAALEIKTAERDFHYLC
                     G"
     gene            complement(1744..2463)
                     /gene="rnc"
                     /locus_tag="PLT63_10585"
     CDS             complement(1744..2463)
                     /gene="rnc"
                     /locus_tag="PLT63_10585"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOD99208.1"
                     /translation="MNREECSHWRALTATLGYVFQDHRLLMNACTHRSFTNENPSAPY
                     SDNERLEFLGDAVLQLCISDELMNRFPAYTEGQLSKMRAAVVNEQSLANLARKFGLGE
                     CLLLGKGEESSGGREKSSLLADAFEAVIAAVFLDGGYERASVLIRNLFAQLITEGSGA
                     GPHFQDYKSALQEYCQNRFHETPRYLLQSESGPDHDKTFVVEIQIAGMMETTGVGKSR
                     KEAEQQVAKKAWDFFTAAAEP"
     gene            complement(2456..>3772)
                     /gene="mtaB"
                     /locus_tag="PLT63_10590"
     CDS             complement(2456..>3772)
                     /gene="mtaB"
                     /locus_tag="PLT63_10590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HOD99209.1"
                     /translation="LGCKVNQYESAAMAECLTGAGYRLVDFHAAADCYIINTCTVTNG
                     ADYQSRQLIRRAQRLNPQAAIIVTGCYAQTTPHVFAGMPGVAVVVGTEEKHRMREIIA
                     AVREKFSEGMEASLAPEVQVAPIDGYREANIPPVSRFSGHTRAFLKIQDGCNAFCSYC
                     IVPFARGRSRSIGAAEVIERIENLARRGYREVVLTGIHLGVYGEDRRDGSTLAKVLRH
                     VEDYQLVERLRLSSLEPREISDEILGIMSTARIVCPHLHIPLQSGDDGILTKMGRNYD
                     RIFFQALLEKAVSLRPDMTVGMDVMAGFPGEDMKAFENTINLVTSAPIAYLHVFPYSR
                     RSGTAAARMGNQVREEEKKRRCLILRDLGTNKRRQHMEKFVGAVLPVLVEEREKRDGG
                     RFQGLSHNYLSVVFKKGSAVMTNTIMPAKIAALANDHSLLGAPVHE"
BASE COUNT          705 a         1125 c         1165 g          777 t
ORIGIN      
        1 atgatcttct tctgcgagtc tcgctccaca tttatggtcg cctcgatgcg gatcagttcc
       61 ttcccctcct cttccttgaa ggcatccacg acgacggcgg tggcgtaagg gatctctttg
      121 tgggtatagg agatgatctt ctccctgatt atttcggcgg cgataaatct ctccggacgg
      181 tcggtgagca tgtcttccgg aaaataggga gggccggcgg ggaggagcga ccggatttcc
      241 tgaaggagga ccgggacgcc gtcgccggtg cgggcgcata ggggaatgaa ggcgcggcag
      301 gggaagatcg tccgggccgc gtcgatgagg ggcaggaggg cgtctttttt taccagatcc
      361 atcttgttga cggccaagat tgcccgttgc cggtaagatt gaagcgtcgc cagcagcgcc
      421 cggtcctcgg gggggatgtc ctcgcccgcc tcgatgagca ccagcagcac atctacgtct
      481 cccgccacgt tgcgcgccgt tttcaccatg gcggcgttca gaggcttatg aggatcgtga
      541 atccccgggg tgtccaagaa gatcatctgg ccatcggcct gatgtttgat ccccatgatc
      601 cggttgcggg tcgtctgggg tttggggctg gtaatggcga tgttctcctc caggatactg
      661 ttgaggaggg tcgatttgcc cacgttgggc cgtcccacga taccgatgaa tcccgatttg
      721 aacatctaaa cctcctcttt caaccacaga gataatggaa gtcccgctcc gccgttttga
      781 tttccaaggc ggcgaggcgg cagtggttcc gtaagaactt gatgttgcag ttttcatgtc
      841 cgcgaaaggc gctttcctcc ccggggggga caaaaaagac cgccttccgg ccctcgagat
      901 cggcgccccc aagaagggcc agggcccgtt cccgccagcg cgccgaagcg accagggccc
      961 cgaaggcggg atgatagggg ccacccagta cggcgccggg gccgctgagg atatccgttg
     1021 cctggagacc caggcggatg atggcgatgc ccgcattttc caggatgtcc cacgcctgcc
     1081 ggcagagatc gacggcttcg gggagcgtta aaggcacgta ccgcccctga agaaaatcct
     1141 ccgccagggg gctgccggcg aggaccaggg tgggatggat acgcacacct tggggtccaa
     1201 gcgctgtcgt ccgggaaacg gtatccagga aggcgccgcc atcgtcgccg ggcaggcccg
     1261 ccatgagatg gaggatgacc tccagccccc ggtgcttgag catcgccacg gcggcgacta
     1321 catcccgggc ggtatgcccc cggccggagg cggccagtac gccgtcgacc agggactggg
     1381 cgccgatttc cacggtccgg accccgaagg ccgccagtcg ttccacatcc ttgtcgcgca
     1441 ggcaatccgg acgggtcgag acgcggatcc ctacgacggt gccgttttcc atatgccgcc
     1501 gggccatgtg gaggagatct tcctgttctt ccggggtcag gccggtgaaa ttgccaccgt
     1561 aaaaggcgat ctccaccggt tgcccgggtt ttttcccccg cgctgccgag gtgagaaacg
     1621 cccgaacctt ttcgtggaaa tagcccgccc ccagctttcg gggatcagca ccgccggaga
     1681 tctcctggtt gcagaaacgg cagcggtggg gacagccgcg attacggagg aaaaagggga
     1741 tgatcatggc tcggcggcgg cggtgaagaa atcccaggcc ttttttgcca cctgctgctc
     1801 cgcctccttt ctgctcttgc cgacgccggt ggtctccatc atcccggcaa tctggatttc
     1861 caccacaaag gtcttgtcat ggtccggtcc ggattcggac tgcaacaggt atcgcggtgt
     1921 ctcgtggaac cggttctggc aatattcctg gagggccgat ttgtaatcct ggaaatgggg
     1981 gccggcaccg ctgccttcgg tgatcaactg ggcaaacagg tttctgatca gcacagacgc
     2041 ccgttcatag ccgccgtcga gaaagaccgc ggcgattacg gcttcgaagg cgtcggcgag
     2101 gagagaggat ttctcccggc cgccggagga ctcctctcct tttcccaaga gcaggcactc
     2161 gcctagaccg aactttctcg ccagattcgc cagggattgc tcattgacaa cggcggccct
     2221 catcttggag agctgacctt ccgtataggc gggaaacctg ttcatgagct catcgctgat
     2281 gcagagctgc aggacggcgt ccccgaggaa ttcgagccgt tcgttgtcgg agtagggcgc
     2341 cgagggattt tcattcgtaa aggaacgatg ggtacaggcg ttcatcagga gacggtgatc
     2401 ttggaaaacg tatccgaggg tcgcggtcag ggcccgccag tgcgagcact cttccctatt
     2461 catggacggg cgcccccaaa agcgagtgat cgttggccag ggcggcaatt ttcgccggca
     2521 tgatggtgtt ggtcattacc gcgcttcctt tcttgaagac caccgaaaga tagttgtggg
     2581 acaagccctg aaagcgaccg ccgtctcgct tttctcgctc ttctaccagg accggtagga
     2641 cggcgccgac gaacttttcc atatgttgcc ggcgtttgtt tgtgcccagg tccctgagga
     2701 tgaggcagcg cctctttttc tcttcctccc gcacttgatt gcccatccgg gccgccgcag
     2761 ttcccgagcg ccgggagtag gggaagacat gaaggtaggc aatcggggcg gaggtaacca
     2821 aattaatcgt attttcaaag gctttcatgt cttcgcctgg aaagcccgcc atgacgtcca
     2881 tcccgacggt catgtcgggg cggagggaga cggccttttc cagtaacgcc tggaaaaata
     2941 tccggtcgta gttgcggccc atcttggtca ggatgccgtc atccccgctc tggaggggga
     3001 tatggagatg gggacatacg atccgggcgg tgctcattat ccccaggatc tcatcgctga
     3061 tttcccgggg ctccagagag ctcaaccgca aacgttccac aagctgatag tcttccacgt
     3121 gcctcaatac cttagccagg gtggaaccgt cccggcggtc ctcgccatag accccgagat
     3181 ggatgcccgt cagcaccacc tcccgataac cgcgccgggc caggttttcg atgcgctcga
     3241 ttacttccgc cgctccgatg ctgcggcttc gcccccgggc aaaggggacg atgcaataac
     3301 tgcagaaggc attgcagccg tcctgaatct tcaggaaggc ccgggtgtga ccggaaaaac
     3361 ggctgacggg agggatattc gcctcccgat agccgtcgat cggtgcaacc tgcacctccg
     3421 gtgccaggga cgcctccatc ccctccgaaa acttttctcg tacggcggcg attatttccc
     3481 tcatccggtg tttctcctct gtcccgacga ccacggcgac ccccggcatc ccggcaaaaa
     3541 catggggtgt ggtctgggca taacagccgg tgacgatgat ggccgcctgg gggttgagcc
     3601 tttgcgctcg ccggatgagc tgccgcgact gataatccgc gccgttcgtg accgtacagg
     3661 tgttgatgat ataacagtcc gcggcggcgt ggaaatccac gaggcgatag cctgctccgg
     3721 tcagacattc cgccatggcc gccgattcgt attgatttac cttgcatccc ag
//
LOCUS       DAOSMF010000175         3757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_26566_length_3757_cov_24.215289, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000175 DAOSMF010000000
VERSION     DAOSMF010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3757
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26566_length_3757_cov_24.215289"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1070)
                     /locus_tag="PLT63_10595"
     CDS             complement(<1..1070)
                     /locus_tag="PLT63_10595"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOD99210.1"
                     /translation="MENFKNKPMYSYLMVLVICASAGLQGWMALFTNFAKEIVGVNGF
                     QIGVAQAVREIPGFLTFLVMYMLLIMREHRLSAWSVVLLGIGIGATGFFTTFPGLLMT
                     TIVMSIGFHYFETTNKSLTVQYFDRHDAPIVFARLRGYGALANITVGVVVWMLSFFIP
                     YRGNFLLLGIFVGLAGIYMLTKNPAEEEGLHRQNKLVLRRKYWLYYTLNFLSGARRQI
                     FIVFAVFLLVEKYRLAVSTIAGIYVLNYALTFLTNRYISRAINVYGERVVLSLESASL
                     FILFLGYAFIENSWVAVILYVLDSVFFNFSIGLNTYLQKTADTSDLSQSTAMGFTINH
                     ISAIIIPLIGGSLWLLNWRLPFL"
     gene            complement(1156..1440)
                     /locus_tag="PLT63_10600"
     CDS             complement(1156..1440)
                     /locus_tag="PLT63_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOD99211.1"
                     /translation="MPIYEYKCKKCGKDFEMFQRITDPAIKSCRFCKGPVTKLMSLSS
                     FHLKGSGWYVTDYGGVKAPHSESKQEEQSAAAAVTEEVKSDTSGDAKTSE"
     gene            complement(1464..1991)
                     /locus_tag="PLT63_10605"
     CDS             complement(1464..1991)
                     /locus_tag="PLT63_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99212.1"
                     /translation="MGRYFNTAQISLTNSAFQHAERLIGDYFRMSLEKIRNHRYDVRT
                     LAFLDKHEVKEGAFAHLCKYRYGKDGEPNEGGLHFYRICLQDDGILDAVERAHSFIKL
                     SPLLLYIAAHELVHVVRFDNGEISFEMSAAEKREEEEKVHGITREVLQAALKPDLKLV
                     LDCFSNRYQLGDIYG"
     gene            complement(2139..3623)
                     /locus_tag="PLT63_10610"
     CDS             complement(2139..3623)
                     /locus_tag="PLT63_10610"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOD99213.1"
                     /translation="MNAISSEAKVGLFVLIGLIVLGYMSFQVGQRGFSFQRGYLLDVE
                     FDNAAGLATGASVQIAGVEVGRVETIRLKNGRALVSIRLPGEVKLERDVTAAIKTHGI
                     LGDKYIEVIPGTQGTALLQAGESITRVERQADVDKLLNQLASIAEDVKTVTGSLSKVM
                     GGRAGEESMGAILENTRLLTRNLNAVVMNNEANLRAMLENTRQLTGNLNRVVVQNDEK
                     IAQVIESLRGASSQMEKTFVALNDITAGIQRGEGTVGQLVKDKQMAEKLHKTMASLQE
                     VSDKINKGQGTIGKLVNDDETVNNLNKTLGGINKFVNKSEQFRTFISYRGEYLTDKSD
                     AKSYLDLRIQPRQDYFYLLGLAVDPRGRRTVKDITQGGATTTITEWDRSGLLFNAQLG
                     KRFQDVVLRGGIFESTGGVGLDFLTFQDKLRLTFEAFDFSVDRKAHLKAGGEIRLLKN
                     IYLNAGWDDFISDQGNSSPYVGVSLRFEDEDLKYLLTSVPVPTK"
BASE COUNT          812 a         1075 c          999 g          871 t
ORIGIN      
        1 agaaagggca gccgccagtt caatagccac aggctgccgc cgatcagggg gatgatgatg
       61 gcggagatgt ggttgatcgt gaatcccatg gcggtgctct gggaaaggtc gctggtgtcc
      121 gccgttttct ggagatacgt gttcaaaccg atggaaaagt tgaaaaagac actgtccagg
      181 acgtagagga ttaccgccac ccaggaattc tcgataaacg cataacccag aaacaggatg
      241 aagagactgg cgctttccag ggataggacc acccgctcgc catagacgtt gatcgcccgg
      301 gagatatatc tattggtgag aaaggtcagg gcatagttca ggacataaat gccggcgatg
      361 gtgctgacag caagacggta tttttccacc agaagaaaga cggcgaagac gatgaatatc
      421 tgccggcggg cgccgctcag aaagttcagg gtatagtaca accagtattt ccgacgcagg
      481 acgagtttgt tttggcggtg cagtccctcc tcttccgccg gatttttcgt cagcatatag
      541 atacccgcca ggcccacaaa gatccccagc aggaggaaat tgccgcggta gggaatgaaa
      601 aaggaaagca tccagaccac caccccgacg gtgatattgg ccagggcgcc gtatccccgc
      661 aggcgggcaa agacgatggg cgcgtcatgc cggtcgaaat actgcaccgt cagggacttg
      721 ttcgtggtct cgaaatagtg aaaaccgatg gacataacta tggtggtcat gagcaggccc
      781 ggaaaggtgg tgaaaaagcc ggtggcaccg ataccgatcc ccagcagcac cacggaccac
      841 gccgacaggc ggtgttcccg catgatcagc agcatgtaca tcaccagaaa ggtcaggaag
      901 cccggaatct cacgtaccgc ctgggccacc ccgatctgaa aaccgtttac gcccactatc
      961 tctttggcaa aattggtgaa caaggccatc catccctgca atccggcact ggcacagata
     1021 acgagcacca tgagatagga atacatcggt ttgttcttga agttctccat gacgaaaatg
     1081 aaaaaccgga gacagcctca gggggcacat ctccggttat gtattcgtga ttataatttt
     1141 ttgtcgcgat gccggttatt cggaagtctt ggcgtcgccg gatgtatcgg attttacctc
     1201 ttccgtcacg gccgcagccg ccgactgctc ctcttgtttc gattcactgt gcggcgcctt
     1261 taccccaccg taatcggtca cgtaccagcc gcttcccttt agatgaaacg aagaaaggga
     1321 catcagcttc gtcacagggc ccttacagaa ccggcaggat ttgatcgccg gatcggtaat
     1381 ccgctgaaac atttcaaagt cctttccgca tttcttgcat ttgtactcat aaattggcat
     1441 aaaacaccct cccaattttg attttacccg tatatatctc ccagttgata gcggttgctg
     1501 aagcaatcca acaccaactt cagatcgggt ttcagcgccg cctgcaatac ctcgcgggtg
     1561 atcccgtgga ccttttcctc ctcctcccgt ttctccgccg ccgacatctc gaaagagatc
     1621 tcgccattgt caaaccgaac cacgtgcacc agttcatggg cggcaatata aagcagcaga
     1681 gggctcagct tgatgaagga atgggcccgt tccacggcat cgaggatccc gtcgtcctga
     1741 aggcagatcc ggtagaagtg taaaccgccc tcattcggct ctccatcttt gccgtatcga
     1801 tatttgcaca gatgggcaaa ggcgccctct ttcacctcat gcttatcgag aaaagccagc
     1861 gtccgcacgt cataacggtg gtttcggatc ttttccagac tcatccgaaa ataatcgccg
     1921 atgagcctct ccgcgtgttg aaaagccgaa ttcgtcaagg atatttgtgc ggtattgaag
     1981 taccgtccca tggtccctca ctgcggaagc cgatgattac cggtaacggg cgccccacta
     2041 tcgtcctttt tcgttcccta cgtaagcctt cacgcttgtt ggcggcaatt tatgcatttt
     2101 ctccaccttg tcaagcatta tttttccttt ttatttcatc attttgtcgg caccgggacg
     2161 gaggtgagga gatacttgag gtcctcgtcc tcgaaacgga gcgacacccc tacatagggg
     2221 gaactgttac cctggtcgct gatgaaatca tcccagccgg cgttgaggta aatgttcttc
     2281 aggaggcgga tttctccccc ggccttgaga tgggccttcc ggtccacgga gaagtcgaag
     2341 gcctcgaagg taagccgcag cttgtcctgg aaggtgagaa agtccaggcc gacccctccc
     2401 gtggattcga atatgccgcc gcggagtacg acatcctgaa aacgcttgcc caactgggca
     2461 ttgaacaaga gaccgctccg gtcccactcg gtgatggtcg ttgtcgctcc tccctgagtg
     2521 atgtccttga cggtccggcg tcctcggggg tccaccgcca ggccgaggag atagaagtag
     2581 tcctgacgcg gctggatgcg cagatcgaga taactcttgg cgtcgctctt gtcggtgagg
     2641 tattcccccc ggtagctgat aaaggttctg aactgctcgc ttttattaac aaacttattt
     2701 atccctccca gggttttgtt caggttgtta accgtttcat cgtcgttgac gagcttgcct
     2761 atggtcccct ggcccttgtt gatcttgtcg gagacctcct gcagactggc catggtttta
     2821 tggagttttt ccgccatctg tttgtctttg accagttggc ccacagttcc ctccccgcgc
     2881 tggataccgg cggtgatgtc gtttagggca acaaaggtct tttccatctg gctggaagcc
     2941 ccccggaggc tctcgatgac ctgggcgatc ttctcgtcat tctggacgac gacccggttc
     3001 agattgcccg tcaactggcg ggtgttttcc agcatggccc ggagattggc ctcgttgttc
     3061 atgaccacgg cattgaggtt gcgggtcagc agccgggtgt tctccaggat cgcccccatg
     3121 gattcctcgc cggccctgcc gcccatgacc ttgctcaggg agccggtaac cgttttgacg
     3181 tcctcggcaa tggaagcgag ctgattgagg agtttgtcca cgtcggcctg gcgctccacc
     3241 cgggtgatgc tctcgccggc ctggagcagc gccgttccct gggtgcccgg aatcacctcg
     3301 atatatttgt ccccgaggat gccgtgggtc ttgatggccg cggtaacatc tctttccagc
     3361 ttcacctcgc ccggcaggcg gatactcacc agcgcccggc cgtttttcag acggattgtc
     3421 tccacccgcc ccacctcgac gccggctatt tggacggagg cgccggtggc gagaccggcg
     3481 gcattgtcga actccacatc caacagatag ccccgttgga agctgaagcc ccgctgtccc
     3541 acctgaaagg acatataacc gaggacgatc aggccgatca aaacgaacag accgactttc
     3601 gcctcggagc tgattgcatt catccgctcc ccctcattca cattatccgt tcagttcccc
     3661 ttggcgggga tgacatgcca aggcatccgt tcccgaaggg tttccttcac atgatccgga
     3721 tcggcccctc gatgcttccc gcaagaaact gtttgat
//
LOCUS       DAOSMF010000176         3701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_27081_length_3701_cov_20.549095, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000176 DAOSMF010000000
VERSION     DAOSMF010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27081_length_3701_cov_20.549095"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="PLT63_10615"
     CDS             <1..685
                     /locus_tag="PLT63_10615"
                     /inference="COORDINATES: protein motif:HMM:NF012688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HOD99214.1"
                     /translation="SADYSQIELRVLAHLSEDPTLVAAFRAGEDVHSRTASTIFGVFP
                     EFVNEEMRRQAKVINFGILYGMSAFGLAKELELNQKLAQAYIDEYFRRYAGVKSYLDG
                     VLEQARRDGYVCTLMNRRRYLPDLNAANANIRQFAERMAVNTPIQGTAADLIKMAMIR
                     IDGRLAAQGLTAAMIIQVHDELVLEAPEAEVAAVTALLREEMEQVMSLRVPLKVAVHM
                     GRNWDDAHQ"
     gene            935..2824
                     /locus_tag="PLT63_10620"
     CDS             935..2824
                     /locus_tag="PLT63_10620"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HOD99215.1"
                     /translation="MDRICRPNISRDKSPHHDTVKTFRWHPGRTVIHLLLAAFLIAAF
                     GSPAAGTTDAPVRNSGGNAANASASPTDKNNQKAPAGGAKINTPPAQTAVDVPAPEYG
                     ADYTEIQKAVVDRLVESRDRNGKPATTAREKFYHERNHNPAWIREGIISSQVRTLVQV
                     LKEAQQEGLNPEDYGVTHIEKLTARLEEVTRAGAAARSETWAEWDVLLTDAFFLYGLH
                     VSEGRLDPGKWFYQWVPYRRTTDLVKILTEALKGDDVEGALRRLPPRYPAYGQLRTEL
                     AKYRRIAAQGGWKPIPEKLKLHRGSTGTAVNLLKQRLVVDGDLDPADLGRNNHFDRQL
                     EAALQRFQHRHGLRANGITDDDTRKALNFTVEKRIRQIEWNMERWRWLPDELGPRYIM
                     ANIPDFRLFVVENQVTVMSMKTVVGKVTQPTPVFMDIMSYLELNPTWNVPNSIVAEEM
                     VPKAQKDPEFLVKKRIRIYSNWSPTAKEIDPKTIDWKKVNPKKFPYRMIQDSGVNPLG
                     RIKFMFPNQFDVYLHDTTQKSLFQRSRRMYSHGCIRVEKPYELGEWVLKDDPAWSKER
                     LIKEIRKGKRQQINLPKPIPVYVIYLTAWFDTRGHIHFRDDIYDYDKNLEKALEQSSG
                     TAGRL"
     gene            2890..>3701
                     /locus_tag="PLT63_10625"
     CDS             2890..>3701
                     /locus_tag="PLT63_10625"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOD99216.1"
                     /translation="MKEIVTDKFVAISDRRFHYREWGDAAGPTLVLVHGIGDDSHVWD
                     DFSQGAAARFRIIAPDQRGHGTSDWARPPAYRCEDYVSDLEQLIAGLELPEVILMGHS
                     MGALHATCYAARHPDRVIALIHADIEPRPPDWNKKYLDNLYERLPRDYHCIGAYVEEL
                     RKNSPYAEEDLLCRLAGHALKETGQGNWRLRYDREVLRHFDRYDLRPRLQEIRCPALI
                     IRGIESRVMSADKAREMSAAIPQGSFAEIQRATHPLHTDNPEEFQRVVMAFLS"
BASE COUNT          838 a         1125 c         1060 g          678 t
ORIGIN      
        1 ctccgccgac tattcccaga tcgagttgcg ggtcctggcc cacctctcgg aggatccgac
       61 cctcgttgcg gccttccggg ccggcgagga cgtccacagc cgcaccgcct ccaccatctt
      121 cggcgtcttc cccgagttcg ttaacgagga gatgcgccgt caggccaagg tgataaattt
      181 cggcattctc tacgggatga gcgccttcgg tctggccaag gagctcgaac tcaaccagaa
      241 gctcgcccag gcctacatcg atgaatactt ccggagatac gccggggtga aaagctacct
      301 ggacggggtg ctggaacagg cccgccggga cggctacgtc tgcaccctca tgaaccgtcg
      361 ccgctacctc cccgacctca atgccgccaa cgccaacatc cggcagttcg ccgagcggat
      421 ggccgtcaac acccctatcc agggcacggc ggcggacctc atcaagatgg ccatgatccg
      481 catcgacgga aggctggccg cccaaggact aacggccgcc atgatcattc aggtccacga
      541 cgaattggtc ttagaggcgc cggaggcgga agtggcggcg gtgaccgccc tcctccggga
      601 ggagatggaa caggtcatgt ccctgcgggt gcccctgaag gtggccgtcc acatgggccg
      661 caactgggat gacgcccatc aatgaaacta tttcattgac tttttcccta tcttggggca
      721 ggaaaagtcc cgcccgctcc gagccgggat ttatcatccc ggctccttgc tttgggcacg
      781 gggcggtgca gcaacacctt gttttcattt gcgttagcat aaaatacatc cgtccgatct
      841 ccctcggaac gttttcttag ccggggttgt tggatcgacg tgaccattta ttatatttca
      901 ttactcagag aggattttct ctcaacggca gacaatggac aggatatgca ggccgaacat
      961 aagcagggac aaatcaccac atcacgacac cgtaaagact tttaggtggc atccggggcg
     1021 tacggtcatc cacctccttc ttgccgcctt cctgatcgcc gccttcggct ctcccgcggc
     1081 tgggacaacg gatgcccccg tccggaacag tggcggcaat gccgcgaacg ccagcgcgtc
     1141 gcccaccgac aagaacaacc aaaaagcccc cgccggcggg gcaaagataa ataccccccc
     1201 agcgcagact gccgtcgatg tccccgcccc ggaatacgga gcggattata cggagataca
     1261 aaaggccgtc gtggatcgcc tggtcgaaag ccgggaccga aacggcaaac cagctaccac
     1321 ggcgcgggag aaattctatc atgagcgcaa tcataatccc gcctggataa gggaagggat
     1381 catctccagc caggtccgga cactggttca ggtcctgaaa gaggcccaac aggaagggct
     1441 caaccccgag gactacggcg tgacccatat cgagaagctg acggcccgcc tggaggaggt
     1501 gacccgggcc ggggcggccg cccgttcgga gacctgggcc gaatgggatg tactgcttac
     1561 cgacgccttc tttctttacg ggctccacgt ctccgagggg cgtctcgatc cggggaaatg
     1621 gttctaccag tgggttcctt accggcggac caccgacctg gtcaagatcc tcacggaggc
     1681 cctgaaaggc gacgatgtag agggcgcctt gcggcgtctt cccccccgct atccggccta
     1741 cggacaatta cggacggaac tggccaagta ccggcggatc gccgcccagg gagggtggaa
     1801 accaatcccg gaaaagctga agttgcaccg tgggagcacc ggcacggcgg tcaatctcct
     1861 caagcagcgg ctggtggtgg acggcgatct cgacccggcg gatttgggca ggaacaacca
     1921 tttcgaccgg cagttggagg cggccctcca gaggtttcag catcgtcatg gcctccgggc
     1981 gaacggcatt accgatgacg atacgcgcaa ggcactgaat ttcaccgtgg aaaaacgaat
     2041 ccgccagatc gagtggaata tggaaagatg gcgttggctt cccgacgaac tgggcccgcg
     2101 gtatatcatg gccaacattc ccgacttccg cctcttcgta gtggaaaatc aggtcacggt
     2161 gatgtccatg aagaccgtgg tgggaaaggt cacccagccc accccggtct tcatggacat
     2221 catgagctat ctggaactta atccgacctg gaacgttccc aattccatcg ttgccgaaga
     2281 aatggttccc aaggcccaga aggatcccga gtttctggtg aaaaaacgga tccggatcta
     2341 cagcaactgg agccccacag ccaaagagat cgatcccaaa acaatcgatt ggaagaaggt
     2401 taaccccaaa aaatttccct atcggatgat ccaggattca ggcgtcaatc cgctgggacg
     2461 gatcaagttc atgttcccca accagttcga cgtatatttg cacgacacta cacagaaaag
     2521 tctattccag cggtcacggc ggatgtattc ccacggatgc atccgggtgg aaaaacccta
     2581 cgagttgggc gaatgggtcc tcaaagacga tccagcctgg tcgaaggagc ggctcatcaa
     2641 ggaaatcagg aaaggaaagc gacaacagat caatctaccc aagcccattc ccgtgtatgt
     2701 catctatttg acggcgtggt ttgacacccg ggggcacatc cacttccggg acgacattta
     2761 cgattacgac aagaacctgg aaaaggccct ggaacagtct tccgggaccg ctggacgcct
     2821 ctgaagacca cgaacgggac gattatgaag accacgaact cggcataaca cgtcgccgct
     2881 ctaccgctca tgaaggaaat agtaaccgac aagtttgttg ccatcagtga tcggcgcttc
     2941 cattaccggg aatggggtga cgccgccggt ccgacgctgg tgttggtcca cggcatcggt
     3001 gacgacagcc atgtctggga tgatttctcc caaggggccg ccgcccggtt ccgaatcatc
     3061 gccccggacc agcgcggcca cgggacaagc gactgggcgc ggccaccggc gtatcgttgc
     3121 gaagactatg tatccgacct ggaacaactg atcgccgggc tggaattgcc ggaagtcatc
     3181 ctcatgggac actccatggg ggccctccat gccacctgtt acgccgcccg acatccggac
     3241 cgggtcattg ccctgatcca tgccgatatc gaaccccgtc cacccgactg gaataagaag
     3301 tatctggaca acctctacga gcggttgccc cgcgattacc attgtattgg tgcgtatgtg
     3361 gaagaactgc gaaagaacag cccctatgcc gaggaggatt tgctctgccg tctggctgga
     3421 catgccctga aagaaacggg gcaaggcaac tggcggttgc gctatgaccg ggaggtcctg
     3481 aggcatttcg accgctatga cctgcggccc cggttgcagg aaatccggtg ccccgccctg
     3541 atcatccggg gtattgaaag ccgggtgatg agcgccgaca aggcgaggga gatgagcgcc
     3601 gccatccccc agggctcctt tgcggaaatc cagcgggcca cccacccgct ccacacggac
     3661 aatccggagg aattccagcg ggtggtcatg gcctttcttt c
//
LOCUS       DAOSMF010000177         3686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_27221_length_3686_cov_17.921234, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000177 DAOSMF010000000
VERSION     DAOSMF010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3686
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27221_length_3686_cov_17.921234"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            296..>1325
                     /locus_tag="PLT63_10630"
     CDS             296..>1325
                     /locus_tag="PLT63_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015903581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOD99217.1"
                     /translation="MSDMFILNGKETPFTPGEMILTAARRGGVEIPTLCYLKDATPTG
                     ACRICLVEVAGARSLMAACSTPVTPGMEVSTESERVHRTRKLNVELLLASGTHNCLVC
                     EANGACRLQDLAYFYRIEKLQFPEKEKEAVYPTEEENPFIVRDFSRCILCGRCVQACN
                     ELVVNRAISQGYRGAKSKIVTGGDRPYNDSECVFCGQCVEVCPVGALTEKKGKRKGRP
                     WETSKVRTTCPYCGTGCQQILHVKGEQIVKITAAADGAPNYGRLCVKGRFAYDFIYSE
                     DRLKTPLIREADGGFREASWDEALDLVAARFQEIIKESGPDAVAGISCARSINEDSYN
                     MQKLFRAVF"
     assembly_gap    1326..1425
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1426..2715
                     /locus_tag="PLT63_10635"
     CDS             <1426..2715
                     /locus_tag="PLT63_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014956451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOD99218.1"
                     /translation="KKTVMNYPPEKAAEISGVPAAKIVAIAREMAAVRPSMLIYTLGI
                     TEHTCGTNNVMSCANLQMLLGNVGMEGGGVNPLRGQNNVQGACDMGALPGVFPGYQSV
                     AVPANREKFARAWGVDGLPDQPGLMIPDMMEGLLTKKIRALWVFGENLANAEPNISHA
                     EKCLETADFLVVQDIFPNETTKFAHVILPSAAWCEDEGTFTSFERRVSMVRKIKSPPG
                     VARPNWWIFKELARKLGHDWPSASGREICDNELAVLCPMFAGIKFCRIEEDGIQWPCP
                     GEDHPGTPIVHKDGNFICGLGVLKGIEWTPPTEVADAEYPLVLSTGRRLSQYHTRTQT
                     GRSGMDAIYDREYADISSADAQEQGIEDGEMIVVKSRRGKVTVPARVTDAVPKGLVWM
                     TFHYREGNCNWLTNNVGDTVTKTPEFKACAVRIEKPA"
     gene            complement(2782..3219)
                     /locus_tag="PLT63_10640"
     CDS             complement(2782..3219)
                     /locus_tag="PLT63_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HOD99219.1"
                     /translation="MTEDSANKEWVPKIVAIVCNWCTYAGADLAGISRIQYPPNVRIV
                     RVPCTGRINPFYIVKALQAGADGVLVSGCHPGECHYISGNLVARRKFAMLKSFLDYIG
                     IEGDRTTFTWVSASEGERFAKVIKGVTAKVQALGPARKLVKKL"
     gene            complement(3369..>3686)
                     /locus_tag="PLT63_10645"
     CDS             complement(3369..>3686)
                     /locus_tag="PLT63_10645"
                     /inference="COORDINATES: protein motif:HMM:NF038090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IscA/HesB family protein"
                     /protein_id="HOD99220.1"
                     /translation="MLQVTEKATVVIKEFLQGKDIQSIRIMMTAGCGGPNLGMALDEP
                     KETDEVIKDNGLTFLIDKTLYEDARPITVDFIDSAMGSGFKLSSSLAAIGGGCGGGCK
                     GCG"
BASE COUNT          759 a         1063 c         1050 g          714 t
ORIGIN      
        1 ggttctcgcc gggaattccc gccgctgatt tctaccgctg acccgacagg ccccgacctg
       61 tcctgccgcg gtgcatgggg cggcaggaag gacggaaggc atgaggggtc ttcaaatccc
      121 caaccctgat tccactgaaa atttaacctg aaaccgggat agtcacggat atctggataa
      181 tcgtgacgac tacggttgtt tcctctccag gcttacgcat gtggatcgtc atatcatatg
      241 tgcagttggc cgttgaggcg tatatttgaa taatatcaag aagatggagg caagcatgag
      301 cgatatgttt atcctgaacg gaaaagagac cccctttaca ccgggggaga tgattctgac
      361 ggcggcacgc cggggcggcg tggagattcc cactctttgc tatctgaaag acgccacgcc
      421 tacgggggcc tgccggatct gtctggtcga ggtcgcgggg gcccgctccc tcatggccgc
      481 ctgttcaacc cccgtcacgc cggggatgga ggtgtccacc gagagcgaac gggtccatcg
      541 cacccgcaaa ctcaacgtcg agctcctgct ggccagtggc acccacaatt gtctcgtctg
      601 cgaggccaac ggcgcctgcc ggctccagga tctggcctat ttttaccgga tcgagaaact
      661 gcaattcccg gaaaaagaaa aggaggccgt ctaccccacg gaagaagaaa atcccttcat
      721 tgtccgcgat ttcagccgct gcatcctctg cggacgctgc gtccaggcct gcaatgaact
      781 ggtcgtgaac cgggccattt cccagggcta tcgcggtgcg aaaagcaaga tcgtcacggg
      841 gggcgatcgg ccgtataatg acagtgaatg cgtcttctgc ggtcagtgcg tggaggtgtg
      901 tcccgtcggc gccctgacgg agaagaaggg aaagcgaaag ggtcgcccct gggaaacgag
      961 caaggtccgc acgacgtgtc cctattgcgg tacgggctgc cagcagatac tccatgtcaa
     1021 gggcgaacag atcgtgaaga ttaccgccgc cgctgacggg gcgcccaatt acggccgcct
     1081 gtgcgtgaaa ggccgcttcg cctatgattt catatattct gaagacagac tcaaaacgcc
     1141 gctgattcgt gaagcagatg gcgggttccg ggaggcatcc tgggacgagg ccttagatct
     1201 tgttgcggcc aggttccagg aaataataaa ggaatccggc cccgacgccg tggccggcat
     1261 cagttgcgcc cggagcatca atgaagattc ctacaacatg cagaaacttt tccgggccgt
     1321 tttccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaaaaa gacggtcatg
     1441 aactatccgc cggagaaggc ggcggagata tcgggggtgc ccgcggccaa gatcgtggcc
     1501 attgcccgcg aaatggccgc cgtcaggccg tcgatgctga tctataccct gggcataacg
     1561 gagcatacct gtggtacgaa caacgtcatg agctgcgcca acctccagat gctgctgggc
     1621 aatgtgggga tggaaggggg cggcgtcaac ccgctccggg gacagaacaa cgtccagggg
     1681 gcctgcgaca tgggggccct ccccggtgtc ttccccggtt atcagagcgt tgccgtcccg
     1741 gccaaccggg aaaaattcgc ccgggcctgg ggggtggacg gtttacccga ccaaccgggg
     1801 ctcatgatcc ccgatatgat ggagggcctg ctcaccaaaa agatccgggc cctgtgggtc
     1861 ttcggggaaa acctggccaa tgccgagccg aatatctccc acgcggaaaa gtgcctggag
     1921 accgccgatt tcctggtggt tcaggatatc ttcccgaacg agacgacaaa atttgcccat
     1981 gtgatcctcc cttcggcagc ctggtgtgaa gacgagggga ccttcaccag cttcgagcgg
     2041 cgcgtcagca tggtccggaa gatcaaaagc ccccccggcg tcgcccggcc gaactggtgg
     2101 atcttcaagg aactcgcccg gaagctggga cacgactggc cgtccgcgag cggccgggag
     2161 atctgtgaca acgagctggc cgtactttgt cccatgtttg ccgggatcaa gttctgccgc
     2221 atcgaggagg acggcatcca gtggccgtgc cccggcgagg atcatcccgg gacacctatt
     2281 gttcacaaag acgggaattt catctgcggt ctgggggtcc tcaaagggat tgagtggaca
     2341 cccccgacgg aggttgccga cgcggagtat cccctggttt tgagcacggg ccggaggctt
     2401 tcccagtatc atacccgtac ccagaccgga cggagcggca tggatgccat ctacgaccgg
     2461 gagtacgccg acatctccag cgccgatgcc caagagcagg ggattgaaga cggcgagatg
     2521 atcgtcgtga aatcccggcg gggaaaggtg accgtgccgg cccgggtgac cgatgcggtt
     2581 cccaaaggcc tggtctggat gaccttccat taccgggaag ggaactgcaa ttggctcacc
     2641 aacaacgtgg gcgacaccgt caccaagacg ccggagttca aggcctgcgc cgtccggatc
     2701 gaaaagccgg cttagaaagc ataaagcaag ccttcccgtc ccaaggcggg aaggcttgct
     2761 ttatgcgtcc ccatcttctt cctaaagctt ctttaccagt ttgcgcgccg gccccagcgc
     2821 ctggaccttg gcggtcaccc ccttgatgac cttagcgaaa cgctcgcctt cggaggcgga
     2881 aacccaggta aacgtggttc tgtctccttc tataccgatg tagtccagaa agcttttcaa
     2941 catcgcgaac ttgcggcgcg ccaccagatt tcccgagata tagtggcatt cacccggatg
     3001 acaaccggac accagtacgc cgtcggcgcc ggcctgcagg gccttcacga tgtaaaaagg
     3061 gttgatcctt cccgtgcagg ggacgcgcac aatgcgcaca ttcggtggat actggatcct
     3121 gctgataccg gccagatcgg cccccgcgta ggtacaccag ttgcagacga tggcaacgat
     3181 tttgggcacc cattctttgt ttgctgaatc ttcagtcata tctcctctct ctcattgggc
     3241 aacaattgcc ctggtatggc aactccttta tggccatttc acaactttgc cctcaccgcc
     3301 acgtcggcaa tgcccgcggc aatgatatct ttcctgaagt gcgatggccg cccggatttc
     3361 ccctgatttc aaccgcagcc tttacagccg ccgccgcaac cgccgccgat agccgccagg
     3421 cttgaggaca gtttgaatcc cgaacccatg gcgctgtcga tgaaatccac cgtaatgggc
     3481 cgggcatctt cgtagagggt cttgtcaatc aggaaggtaa ggccgttgtc cttaatcact
     3541 tcatcggttt ccttcggctc atccagggcc atgcccaggt tcggtccccc gcaaccggcg
     3601 gtcatcatga tccggatgga ctgaatgtcc tttccctgaa ggaattcctt gattaccacc
     3661 gtcgcttttt ccgtaacttg caacat
//
LOCUS       DAOSMF010000178         3661 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_27462_length_3661_cov_20.022185, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000178 DAOSMF010000000
VERSION     DAOSMF010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27462_length_3661_cov_20.022185"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="PLT63_10650"
     CDS             complement(<1..234)
                     /locus_tag="PLT63_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415986.1"
                     /note="produces methionine from
                     2-keto-4-methylthiobutyrate and glutamine in vitro;
                     mutations do not affect methionine salvage in vivo
                     however; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HOD99221.1"
                     /translation="MEIADRLTKIPPYLFMELRKKIGAARAAGVDVISLAIGDPVEAT
                     PKSVIDELCRTAYDPANHRYPTDEEKGMLAFRQE"
     gene            complement(378..632)
                     /locus_tag="PLT63_10655"
     CDS             complement(378..632)
                     /locus_tag="PLT63_10655"
                     /inference="COORDINATES: protein motif:HMM:NF013813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99222.1"
                     /translation="MAVRTYERGVEDETLACGTGSVAAALITAGKGLASSPVDVRVKS
                     GETLRIHFEQTGAGFRNVYLEGKTAVICSGRLWPEAYARG"
     gene            complement(653..1219)
                     /gene="dapF"
                     /locus_tag="PLT63_10660"
     CDS             complement(653..1219)
                     /gene="dapF"
                     /locus_tag="PLT63_10660"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOD99223.1"
                     /translation="MTMIEFYKMSGSGNDFILIDNRRRQVEEKLGAGSVVEFVQAVCT
                     PRISLGADGLIIIQESTRADFSWRFFNADGSEVEMCGNGGRCAARFAFLLGIAPERMS
                     FETGAGIIDAEVRDNIVKLRLTDPGDLRIDETIAIGGRDFKVSSINTGVPHVVHYVDD
                     LDACDVFSIGRDIRYHEHYRPGGRTPTL"
     gene            complement(1246..1455)
                     /locus_tag="PLT63_10665"
     CDS             complement(1246..1455)
                     /locus_tag="PLT63_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99224.1"
                     /translation="MIEIADDAPLVSDHEQKEAQRFFSSYFPKDRLREAAESYEDGQE
                     GKLTIMPVPGTMKDENIKLFVDIFM"
     gene            1652..2569
                     /locus_tag="PLT63_10670"
     CDS             1652..2569
                     /locus_tag="PLT63_10670"
                     /inference="COORDINATES: protein motif:HMM:NF024138.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOD99225.1"
                     /translation="MSAELMTTKEVAKYLNINDKQVYALIREGGIPASRATGKWLFPK
                     QLIDAWIESSAREGLSRARRQSEKAAGALLAAGSNDPLLDILQTAIRRIHPELYIFSA
                     NTGSSEGLAALNNGYTDIAWSHLWDPETGRYNTPFIPSRLPRIDAVVVNLFHRDLGLI
                     VAPGNPLGVRTIGDLGKPGLHFINRQDGSGTRLLIDYHLQRAGVDPLRIAGYEREVST
                     HLEVGLAVLSRQADAGVATGSVSRLLGLEFITLVRESFDMILAKETYFKKGVQDFIET
                     LRSDGFRRGVENLGHYDFHASGKVLFAVN"
     gene            2609..3463
                     /locus_tag="PLT63_10675"
     CDS             2609..3463
                     /locus_tag="PLT63_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOD99226.1"
                     /translation="MKKISLLLLAILLVTPPGTAVAKDKKFILMASTIGPIDAGIVGA
                     LEDSFEKEKGIRVRHVGAGTGATLKIAQKCNVDLVMVHARSLEEKFVADGYGTERIPL
                     MYNDFVMVGPAADPAGIKGLKSAAEAMRKIAAAGAPFVTRGDKSGTHVAEMELWKAAG
                     INPAGTWYVVFEKGSEGNVATLRFTDQKRAYTVIDRATFLALQKEVKLAVLVEGDEAL
                     LNRITLIPVNPGKCPQANLKETMIFVDWLTDPNKGQRLIETFGVEKYGKPMFFPDSIP
                     WRQRGGGK"
BASE COUNT          769 a         1048 c         1076 g          768 t
ORIGIN      
        1 ctcctggcgg aaggccagca tccccttttc ctcgtccgtg gggtagcggt gattcgccgg
       61 gtcgtaagcc gtccggcata gttcatcgat gacggacttc ggggtggcct ccacgggatc
      121 gccgatggcc aggctgatga cgtccacgcc agcggccctg gcggcgccga tcttctttcg
      181 cagctccatg aacaggtaag ggggaatctt ggtcaaacgg tcggcaatct ccacggtgtc
      241 tctcctttgc gtcgtgatcg ggttggtgag tcgtgtctat ataggaaaag gacgggaatc
      301 gtcaatagaa acctgccttg agaactgcat gtaaggcggc cccggcaatt gcggcggatt
      361 tcctggtccc ggtggcatca accccgggcg tatgcctccg gccagaggcg gccggaacag
      421 attaccgccg ttttcccctc cagatagaca ttcctgaatc cggcaccggt ctgttcgaag
      481 tggatccgga gggtctcgcc gctttttacc cggacatcca cgggagacga cgccaggccc
      541 ttcccggcgg tgattagtgc ggccgccacg gaaccggtgc cgcaggccag ggtttcatcc
      601 tccacgcccc gctcgtaggt gcggacggcg atcgttcccg gagcgagtac ctttacaaag
      661 ttggcgttcg tcccccgggc cggtagtgct catgataccg gatgtcccgg ccgatggaaa
      721 agacgtcgca ggcatccagg tcgtccacat aatggaccac atggggaacc ccggtgttga
      781 tgctgctgac cttgaaatcc cggccgccga tcgcgatcgt ctcgtctatg cgcagatcgc
      841 cggggtccgt caacctaagc ttgacgatgt tatcccgaac ctcggcatcg atgatccccg
      901 cccccgtttc gaaggacatc cgttccgggg cgatccccag gaggaaggcg aagcgggctg
      961 cacagcgacc cccgttgccg cacatctcca cctccgagcc gtcggcgttg aagaaacgcc
     1021 aggagaaatc ggcccgagtg gattcctgga taatgatgag gccgtcggcg cccagagaaa
     1081 tcctgggagt acagaccgcc tggacgaact caaccacgga tccggcgcct agcttttcct
     1141 ccacctggcg ccggcgattg tcgatgagga tgaaatcatt gccactgccg ctcatcttgt
     1201 agaactcgat catggtcatg tgccctttcc acaatttata ttcctttaca tgaaaatgtc
     1261 cacaaatagc ttgatatttt catctttcat tgtgcccgga acgggcatga tggttagttt
     1321 gccttcctgg ccgtcctcgt acgactccgc cgcttctctt aaacgatctt tcggaaaata
     1381 tgaagagaaa aaacgttgtg cctctttctg ctcgtggtcg gaaacgagag gagcatcatc
     1441 ggcaatttca atcatgtagt taacatggct taatgttatt atttgttgga atcaatcact
     1501 tttcgttgct ttttgtcttg acattccccc gaaaaaatcc gtataaggct atcgactgcc
     1561 gaggtgcggc gggtattctc ttcggccccg atccgcgtcg ataggcaact acagaaatgg
     1621 cgccggtcct tgatagagaa aggtcgtccc gatgtcggcc gagctgatga ccaccaaaga
     1681 agttgccaag tatctgaata tcaatgacaa acaggtttat gccctcatcc gggagggagg
     1741 catccccgcc agccgggcga cggggaagtg gctttttccc aagcaactga tcgacgcgtg
     1801 gatcgaaagc tcggcccggg agggattgtc ccgggcccgc cggcagagtg aaaaggcggc
     1861 cggcgccctc ctggcggcgg ggagcaacga tcccctctta gacatcctcc agacggcgat
     1921 ccgccgaatc catcccgaac tgtacatctt ttccgccaat acgggcagct cggaggggct
     1981 ggcggcgctg aacaacggct acacggacat cgcctggtcc cacctctggg atccggagac
     2041 cggccggtat aacacgccct ttatcccgtc ccgactgccc cggatcgatg ccgtcgtcgt
     2101 caacctcttc caccgtgatc tgggactgat cgtcgccccg ggcaaccccc tgggcgtcag
     2161 gacgatcggg gacctgggaa agccggggct gcacttcatc aaccgtcagg acggctccgg
     2221 gacccgcctc ctcatcgact accatttaca gcgggccggg gtagatccgc tccggatcgc
     2281 cggctatgag cgcgaggtga gcacccatct ggaagtgggt ctggccgtcc tctcccgtca
     2341 ggccgatgcg ggggtggcca cgggttccgt atcccgcctc ctcggactgg aatttatcac
     2401 cctggtccgg gaaagcttcg atatgatcct cgcgaaggag acctatttca agaagggggt
     2461 tcaggacttt atcgagacct tgagatccga cggcttccgc cggggggtag agaatcttgg
     2521 tcattacgat ttccacgcgt cggggaaggt cctgtttgcc gtgaattgac cgagaggatc
     2581 gaaagggaat ttattgttgg agggtgacat gaaaaaaata tctctgctgc tgcttgccat
     2641 cctcctggtc acgccgccgg ggacagccgt tgccaaggac aagaagttta tcctcatggc
     2701 ttccaccatc ggtcccattg atgccgggat tgtcggcgcg ctggaggata gttttgagaa
     2761 agagaaagga atccgggtcc gtcatgtcgg cgccggaacg ggagcgacgt tgaaaatcgc
     2821 ccagaagtgc aatgtcgacc tggtaatggt ccacgccagg tctttggagg aaaaatttgt
     2881 tgccgatggt tatggcaccg agcggattcc cctgatgtac aacgactttg tcatggtggg
     2941 accggcagcg gatcccgcgg ggatcaaagg cctgaagagc gccgcggagg ccatgcggaa
     3001 aatcgccgcc gccggggcgc cgttcgttac ccggggcgat aaatccggaa cccatgtagc
     3061 cgaaatggag ctttggaaag ccgccgggat caatcccgcc ggaacctggt atgtagtttt
     3121 tgaaaaaggc tcggaaggaa acgtggcaac cctgcgtttc accgatcaaa aaagggctta
     3181 caccgttatc gaccgggcta cctttctggc cctgcaaaaa gaagtgaaac tggccgtcct
     3241 cgtggaaggg gatgaggccc tgctgaaccg catcacctta attccggtga atcccgggaa
     3301 atgtccccag gccaacctca aagaaacgat gatcttcgtt gactggctca cggatccgaa
     3361 caagggacag cggctcatcg agaccttcgg ggtggaaaag tacggaaaac ccatgttctt
     3421 ccccgattcg atcccctggc ggcagcgcgg cggcggtaaa tagcggtcgc cgccgcgttg
     3481 tcagccagga cagatacttg gtgcaacaac ccgttgtggg taataatggg aaataagatg
     3541 aggaggtaaa aaagtgaaaa agggtctatt tattttactt gtcctgttcc tgacggcctt
     3601 agccgccgct cccgcggcgg cggtggacgt caagttcagc ggcagttact acgcgatggg
     3661 c
//
LOCUS       DAOSMF010000179         3650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_27565_length_3650_cov_12.885118, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000179 DAOSMF010000000
VERSION     DAOSMF010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3650
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27565_length_3650_cov_12.885118"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2653)
                     /locus_tag="PLT63_10680"
     CDS             complement(<1..2653)
                     /locus_tag="PLT63_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOD99227.1"
                     /translation="MNDKWRDQITLDIIVAMNTALVNLRLYPPTSSMIINTIERLHQT
                     LQGFFAGAPSLVLGEADKVLLIGDQPLSRKDLERPPIAAFLEVILNFGIKSITIGSDV
                     TREDLSAFLEILGEKPVTISREGGLVAVMAARQVGHILIDEKIYIAKDSKQQILARLD
                     IDDEVVVRYLTGGEGAAGADLDIQQLQDLAKDPDWLENVFKQGLNQIMGKRDTLPIGQ
                     LSANMVRMLGILDKVIDRADQEKFVRMIAKSIADLDVDMIGCILSRPLDTIFDGRLFG
                     EVVAAIDEAKFEQVAARMAGMKGGRDALGAGAGIGGVGVGAGGVGGAAGGVSAASPGA
                     AGVMGGAAPGAGVGGPGTAAFGAIPPPSAAAAPGAAGAARYAAPASGAAAAPGYAAPS
                     AAAPAPGFAAGAASGAAAASGFASRTPPSAAPQYAAPPSTTSPSTTSPSATPPSGASG
                     VPGVSAVPPAVPEAYRRLMETEKGRQLRERAEAERLRAEDEKSRKLATLKEKISPLLQ
                     GREECFLDEALMCSMADIVGELAAQEGPETTDAVLDRLAEALRSDRQDVRDQAAAALA
                     QILDRLPERRRQEELNRLADRLRAWIEKETVATTAYRKLCHLLKDLISLRIGDGEYRE
                     ALPLLQLFHRIDYGLLEKNETAQEIAMDIIRGLSTPERLDFLFDRYERLTGEDEEQKD
                     VGRLLAHLGEGSLARLMDILREHTNSDERVKVLHLFIEIGRPVLAIIKNEIHLDRPWY
                     FHRNLAYLLGWIKGETTVESLRALLLHKHDKVRQEALTSLAKVGGGERGPVLLETLPA
                     VDGAFKLELIDRLGRMKYREATPVFLAMMKERPFIVTPARIDLEEALCTALGRIGDPA
                     ALSMLNEISNPGFFTVTTYHKKVKIAAS"
     gene            complement(2944..3330)
                     /locus_tag="PLT63_10685"
     CDS             complement(2944..3330)
                     /locus_tag="PLT63_10685"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOD99228.1"
                     /translation="MKLLLDTHILLWSAGQPDKLPESARALLTSPENSLFFSAASIWE
                     IVIKLGLGREDFKVDPHRLRKMLVVHGYTELPVTAEHALKVDTLPPLHRDPFDRLLLA
                     QARTEGMLFLTNDAAVVKYQESILGV"
     gene            complement(3327..3575)
                     /locus_tag="PLT63_10690"
     CDS             complement(3327..3575)
                     /locus_tag="PLT63_10690"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HOD99229.1"
                     /translation="MQTINIHEAKTHLSRLVEEAAQGKSFIIAKAGKPMVKVSPLAAD
                     ELNGAERLGFMAGEISVPDDFDLLGVPEIMLLFEGKAK"
BASE COUNT          686 a         1188 c         1058 g          718 t
ORIGIN      
        1 gggaggcggc gattttgacc ttcttgtggt aggtagtcac cgtgaagaag ccgggattgc
       61 tgatctcgtt gagcatggat aacgccgccg ggtctccgat ccggcccagg gcggtgcata
      121 gcgcctcctc cagatcgatc cgcgccggcg tcacgataaa cggccgctcc ttcatcatcg
      181 ccaggaacac cggcgtcgcc tcccggtact tcatccttcc caggcgatcg ataagttcca
      241 gcttgaaggc gccgtccacc gccggcaggg tctccagcag caccggtccc ctttccccgc
      301 cccccacctt ggcgaggctg gtcagcgcct cctgccgcac cttgtcgtgc ttgtgcaaca
      361 gcagcgcccg cagcgattcc acggtcgtct cccccttgat ccaccccagc agataggcca
      421 gattccggtg gaagtaccac ggacggtcga ggtggatctc gttcttgatg atggccagga
      481 ccggcctccc gatctcgatg aacaaatgga ggaccttaac ccgctcgtcg ctgttggtat
      541 gctcccgcag gatgtccatc agccgcgcca gggagccctc cccgaggtgc gccaggagcc
      601 gccccacgtc cttctgctcc tcatcctccc ccgtgagccg ctcgtagcga tcgaagagaa
      661 aatccaggcg ctccggcgtc gaaagccccc ggatgatgtc catggcgatc tcctgggccg
      721 tctcgttctt ctccaggagg ccatagtcga tgcggtggaa gagctgcaag agcggcaggg
      781 cctcccgata ttccccgtcc ccgatccgca gggagatcag gtccttcagg aggtgacaga
      841 gcttccggta ggccgtcgtc gccacggtct ccttttcgat ccaggcccgc agccggtctg
      901 caagccggtt caattcctcc tgccgccggc gctccggcaa ccggtcgagg atctgggcca
      961 gcgccgcggc ggcctgatcg cgtacgtcct gccggtcgct ccgcagggct tccgcgagac
     1021 gatcgaggac cgcatcagtg gtttccgggc cctcctgcgc cgccagctcc ccaacgatat
     1081 cggccatcga gcacatcaga gcctcgtcca ggaagcattc ctcccggccc tggagcaggg
     1141 gggagatctt ttccttcagg gtcgcgagct ttcgggactt ctcatcctcc gcccggagcc
     1201 gctccgcttc ggcccgttcc cgaagctgcc ggcccttctc ggtttccatg agccgccgat
     1261 aggcctccgg gacggcgggc gggacggcgg agacgcctgg gacgccagag gcgccggacg
     1321 gtggggtggc ggacggtgag gtggtggatg gtgaggtggt ggatggcggg gctgcgtact
     1381 gtggggcggc ggatggtggg gtacggctgg caaatccaga agcggcagcg gcgcccgacg
     1441 cggcaccggc ggcaaatccc ggcgcggggg cggcggctga cggggcagca tatcccggag
     1501 cggcagcagc gcccgacgcg ggggcggcat atcttgcggc accggcggct cccggggcgg
     1561 cggcggctga cgggggcggg atggcgccaa atgcagcggt accggggccg cctacgcccg
     1621 cacccggagc agcgccaccc attacaccgg cagcgcccgg ggaagcggca ctcacacctc
     1681 ccgcagcgcc acccacaccg cccgcaccca cgcccactcc accgatgccc gcacccgcgc
     1741 ccagcgcgtc ccggccgccc ttcatgccgg ccatccgcgc cgccacctgc tcgaacttgg
     1801 cctcgtcgat ggccgccacg acctccccga acagccggcc gtcaaatatc gtatcgagcg
     1861 gcctgctcag gatgcagccg atcatatcca catccaggtc ggcgatggat tttgcgatca
     1921 tccggacaaa cttctcctgg tccgcccggt cgatgacctt gtccaggatt cccagcatcc
     1981 gcaccatatt cgccgacaac tggccgatcg gcagggtatc ccgtttgccc atgatctggt
     2041 tcaggccctg cttgaagaca ttctccagcc agtcgggatc cttcgccaga tcctggagct
     2101 gctggatgtc caggtccgca cccgccgccc cttccccgcc ggtcaggtac cggacgacca
     2161 cctcgtcgtc gatgtcgagc cgggccagaa tctgctgctt gctgtccttg gcgatgtaga
     2221 tcttttcatc gatgaggatg tgccccacct gccgcgccgc catgaccgcc accagccccc
     2281 cctcccggct gatcgtcacc ggcttctccc ccagaatctc caggaaggcc gacagatcct
     2341 cccgggtcac gtcgctgccg atcgtgatgc tcttgatgcc gaaattgagg atcacctcca
     2401 ggaaagcggc gatcggcggc cgttccaggt ctttccggct caacggctga tcgccgatca
     2461 gcagcacctt gtcggcctcc cccaggacca gggacggcgc gccggcgaag aacccctgca
     2521 gcgtctgatg gagccgctcg atggtattga tgatcatgga gctcgtgggc ggatagaggc
     2581 ggagattgac gagggccgtg ttcatggcga caataatgtc caaagtaatt tgatcccgcc
     2641 atttatcgtt catgccggcc tcggttgtgg tgtgtggcga ttagctggaa atgtgccgat
     2701 ttgatacact aaaaacacca gtagagcaat taaaaatcta atgggggtca ggtctttaaa
     2761 attagcattt ctacccattc cggcatcccg attttcctgt gggccgatgt tcctgtgttt
     2821 ctgtgtctta tgccgtatga cctcgggaaa atgcgctaat cactgtgccc gccggcaagc
     2881 aaatacacct tttctttcgc tcacggcgaa aaatgctaat tttaaagacc tgacccccat
     2941 cttttatacg cccaaaatag attcttgata tttgacaaca gcggcgtcat tggtaagaaa
     3001 aagcatccct tccgttcggg cctgagcaag cagcaggcgg tcgaaagggt ctctatgaag
     3061 aggaggcaac gtatcgacct tcagggcatg ttcagcggtt acgggaagct ccgtgtatcc
     3121 atgcaccacg agcatcttcc tcaaacgatg cggatcaacc ttaaaatctt cgcgcccaag
     3181 cccaagcttg ataacaattt cccagatact tgccgcactg aaaaacaggc tgttttccgg
     3241 tgaagtcaaa agtgcgcgcg ctgattcggg aagcttatca ggctggccgg ccgaccagag
     3301 gaggatatgc gtatcgagga gtagtttcac tttgccttgc cctcaaaaag cagcataatc
     3361 tccggtactc ccagtaaatc aaaatcgtcg ggaacggaaa tctcacccgc cataaatcca
     3421 agtctttcag cgccattcag ttcgtctgca gccaggggtg aaaccttgac catcggcttt
     3481 cctgccttgg caatgatgaa ggatttacct tgcgccgcct cctcgaccag acgggaaaga
     3541 tgcgtcttcg cttcatgaat attgatggtt tgcatagccc acctcgccaa gattagttta
     3601 gtgaacttag tctattggca gcgccggata ttgtcaagaa aaaccatggg
//
LOCUS       DAOSMF010000180         3608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_27967_length_3608_cov_20.983113, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000180 DAOSMF010000000
VERSION     DAOSMF010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3608
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27967_length_3608_cov_20.983113"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            446..2209
                     /locus_tag="PLT63_10695"
     CDS             446..2209
                     /locus_tag="PLT63_10695"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD99230.1"
                     /translation="MTPPDKIVWQDRAGKETRPFLQPIAVAIVCAVFLILILIMGIMD
                     MRRLDQTLVGILENRAQGIVEVVQRLAQENLTNLVQASHHREDADRPFAPIESEAYSP
                     QKLLINALVKLGQEVDIHWKKDRLSAAYLRKFADEQEIWMITVMDRRGRAVFQSRPIP
                     EDFVEGNPSPKISIDLLNQLGREKKVGFIALRRKDGSGTVVIALDPDGLRFWGTKVSV
                     EKALQELGGGQSQGLTYFMINDLQGKTLASAGVLPENWKPGDMPVAEMTTGARAVASR
                     KVAYLGKPLLDIAMPVYLKNQVVGIARMGLERESMERILRENGKNIFIFMVLTALIAF
                     LSMWVLYMNQNRHLAGIVTMERRLEKAERLSALGQLAAGVAHEIRNPLNAISMASQRL
                     KRDFVPADERKEKDFQTITGVIRDEIRRLNGIIEEFLTFSRSRRLELREYPVTEVLQK
                     IVNLLREETAGKGIVLETKWAEPSPVIPMDMDKLQQALLNFIKNAVESISDGGRVTIT
                     VTPERKDGIKIAIADTGCGMTEEEIERIFSPEYTTKEKGLGLGLPLSHEIIRGHGGEI
                     RVTSQKDAGTTFEIILPVEKH"
     assembly_gap    2263..2362
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2386..>3608
                     /locus_tag="PLT63_10700"
     CDS             2386..>3608
                     /locus_tag="PLT63_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOD99231.1"
                     /translation="MKALNILIVEDGQSQREMLRDFLIQEGHNVQEAENGEKGVDLVR
                     NGHFDLLLLDYKMPGMDGMQVLTAVKEINPEIDVVIITAYGTIETAVEAMKAGAIDYI
                     TKPIEFDELLLLVERVAERRILIKENEILKQELREKGVTAEQIVYRSPKMEALINMAG
                     RVAPSRATILIQGESGTGKELLARLIHQLSPRSGRAMLAVNCGALHENLLESELFGHE
                     KGAFTGASARRIGRFEEADGSTLFLDEIGELSAAVQVKLLRFLQEREFQRLGGNQTLR
                     ADVRIISATNRDLDARVKAGAFREDLYYRLKVVTIDLPPLRERKEDIPPLIDHFLRKY
                     AKENGKVIKGISSEARDLLIKYDYPGNVRELENILERAVVIGRDEIISVEDLPFLEDS
                     LTAAEEGKKAEGLLRG"
BASE COUNT          843 a          910 c         1062 g          693 t
ORIGIN      
        1 gttttactgt cggctgacca tgcccgtttg ttcaaccgag taaattatct tcttgccagt
       61 aatacatagc acataccgtg ccacccttcg ccattggttg ttaagtgttg gaaacaaatg
      121 caaatatatt gttgataagc ccttccggca gagtgaatcc gcaccttgcc ggcggcaacg
      181 attgtcggtt tacgggagcg attatttaaa tagttcatac aatacaagaa gttatattga
      241 tgacgacttt gttttgaaaa tcgacaaaaa tgtcggggtc acaattcagc gaagctgaat
      301 catgttcaca attcagcgaa gctgaatcat gttcataatt cagtgaagct cctactgttc
      361 ataatttatg attgactttc cccatgaatc gcctatgatg caacgcgatg aagattatgg
      421 ataaaatcgc cgggataaga aaacggtgac ccctccggat aaaattgtct ggcaggaccg
      481 ggcaggcaag gaaaccaggc ccttcctcca gcccatagca gtggccattg tctgtgccgt
      541 cttccttatt cttatcctca ttatggggat catggatatg cggcgcctgg accaaaccct
      601 ggtgggcatt ctggaaaacc gcgcccaggg catcgtggag gtggtacagc gcctggccca
      661 ggaaaacctc accaatctgg tgcaggcctc ccaccatcgt gaagatgctg atcgtccttt
      721 cgcccccatc gaatcggagg catattcacc tcagaagctc ctgatcaacg ccttggtgaa
      781 attgggccag gaagtcgaca tccactggaa gaaggaccga ctcagcgccg cctatctccg
      841 caagttcgcc gacgagcagg agatatggat gatcacggtg atggacagga ggggtcgggc
      901 ggtatttcag agccgcccca tcccggaaga cttcgtggag ggcaatccct cgccgaagat
      961 ttccatcgat ttgttgaacc aactgggtcg ggaaaagaag gtcggtttta tcgccctgcg
     1021 ccggaaggat gggagcggga cggtcgtcat cgccctcgac cccgacggtc tgcgcttctg
     1081 gggcaccaag gtttcggtgg agaaggccct gcaggaactg ggcggcggcc agagtcaggg
     1141 gctgacctac tttatgatta acgaccttca gggtaagacg ctggccagtg cgggcgtctt
     1201 gcccgaaaat tggaaaccgg gcgacatgcc cgtggcggag atgacaacgg gcgcccgtgc
     1261 cgtggccagc cgtaaggttg cctatctggg caaaccgctt ctcgatatcg ccatgccggt
     1321 ttacctgaaa aaccaggtcg tggggattgc caggatgggc ctggaacggg agagcatgga
     1381 gcggatcctc agggagaacg ggaaaaacat ctttattttt atggtcctca cggcgctgat
     1441 cgccttttta tccatgtggg tcctctatat gaaccagaac cgccatttgg cgggcatagt
     1501 gaccatggag cggcgtctgg aaaaggcgga acgtctctcc gccctggggc agctcgccgc
     1561 cggggtggcc catgagattc gcaatcccct caacgctatc agcatggcga gccagcggct
     1621 caaaagggat tttgttccgg cggatgaaag aaaagaaaag gacttccaga ccattaccgg
     1681 ggttattcgg gacgagattc gccggctcaa cgggatcatc gaggagttcc taaccttttc
     1741 ccggagccgc cgtctcgaac tgcgggaata tccagtgacg gaggtactcc agaagatcgt
     1801 caatctcctc cgggaggaaa cagccggaaa ggggatcgtc ctggagacga aatgggccga
     1861 gccgtcgccg gttatcccca tggatatgga caagctccag caggccctcc ttaacttcat
     1921 caaaaacgcc gtcgaatcga tttccgacgg gggacgggtg accataaccg tgacgccgga
     1981 gcgaaaggac gggatcaaga tcgccatcgc ggataccggc tgcggcatga cggaggagga
     2041 gatcgagcgg atcttcagcc cggaatatac caccaaggaa aaaggtctgg gtctgggact
     2101 gccgctgtcc catgagatca tccgggggca tgggggggaa atccgggtta cgagtcagaa
     2161 agacgcggga acaaccttcg agatcatcct gcccgtggaa aaacattaag gatcctgagg
     2221 gagtggagat atgaaggcgc tgaacatcct gattgtggaa gannnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnaaggatcc tgagggagtg gagatatgaa ggcgctgaac
     2401 atcctgattg tggaagacgg gcagtctcag cgggagatgc tgcgggattt tctgatccag
     2461 gagggtcata acgtccagga ggcggaaaac ggagaaaaag gggtggattt ggtccgcaac
     2521 ggacatttcg acctgctcct tctcgactac aagatgccgg gcatggacgg catgcaggtc
     2581 ctgacggcgg tcaaggagat caatcccgag atcgacgtcg tcatcatcac cgcctacggc
     2641 accatcgaga cagccgtgga ggccatgaag gccggcgcca tcgattacat tacgaagccc
     2701 atagaattcg atgaactcct cctcctggtg gaacgggtgg cggagcgccg gatcttgatc
     2761 aaggaaaacg aaatcctgaa gcaggaactc cgggaaaagg gggtgacggc ggagcagatc
     2821 gtttaccgga gcccaaagat ggaagccctg atcaacatgg cgggccgggt ggcgcccagc
     2881 cgggccacga tcctcatcca gggagagagc gggacgggca aggaactcct ggcccggctt
     2941 atccatcagt tgagcccccg gtccggccgg gcgatgttgg cggttaactg cggcgccctc
     3001 catgaaaatc ttctggaaag cgaacttttc ggtcatgaga agggggcatt caccggggcg
     3061 tccgcccggc gaattggccg tttcgaggag gcggacggga gcaccctctt tcttgacgag
     3121 attggcgaac tctccgccgc cgttcaggtg aaactgctta gatttctgca ggaaagggag
     3181 tttcaacgcc tgggaggaaa tcaaaccctc cgagcggacg tgcggattat cagcgccacc
     3241 aaccgggatc tggatgcccg ggtgaaggcg ggggctttcc gggaggacct ctattaccgc
     3301 ctgaaggtgg taaccatcga cctcccgccg ctccgggagc gcaaggagga cattccccca
     3361 ctgatcgacc atttccttcg caagtatgcc aaggaaaacg gcaaggtaat caagggtatc
     3421 agtagcgagg cccgggatct cctcatcaaa tacgattacc cgggaaatgt gcgagagctg
     3481 gaaaacatcc tggagcgggc cgtggtcatc ggtcgggacg aaatcatctc cgtggaagat
     3541 cttcccttcc tggaagattc cctgacggcg gcggaggagg ggaaaaaagc cgaggggctg
     3601 ctccgggg
//
LOCUS       DAOSMF010000181         3594 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_28123_length_3594_cov_21.451823, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000181 DAOSMF010000000
VERSION     DAOSMF010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3594
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28123_length_3594_cov_21.451823"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            403..2997
                     /gene="feoB"
                     /locus_tag="PLT63_10705"
     CDS             403..2997
                     /gene="feoB"
                     /locus_tag="PLT63_10705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOD99232.1"
                     /translation="MKPKIVIALAGNPNSGKTTIFNKLTGARQHVGNYPGVTVERKEG
                     YCRYGDMEMQIVDLPGTYSLTAYSTEELVARNFLIDEKPDVVVDIIDASNLERNLYLA
                     VQLMELGLPLVLAFNMSDQARAQGHEFDIEKLSSYFGASIVQTVGHKGTGMKELLEAI
                     VAAAKDGASEMNRNDENPSGALLSKATIHSPFRQRPVMDYGREIEEEIARLESLIKLK
                     VTGKDKDNLRWLALKLLENDKAVRARFDTPEINSQLEKSAARIEKMLGEHPETAIAGR
                     RYGFISGACREAVRSTIELRHTISDRIDAVATNRLLGIPIFMGLMYLVFQLTFTLGDP
                     PMKWIEEGFGWLGAQILGWWPKGSESALKSLLVDGIIGGVGGVIVFLPNILLLFLAIA
                     ILEDSGYMARAAFIMDRVMHKIGLHGKSFIPMLIGFGCSVPAIMATRTLENRRDRLVT
                     MLVTPLMSCGARLPIYALIIPAFFPQVWHAPMLWLIYVIGIALAVLSAKLLRTTILKG
                     ESVPFVMELPPYRMPTLKSVLIHMWERGWLYLKKAGTIILCISILLWAMTSYPKKTVF
                     DVDYEADLAKSEGTYLSKVTRLNALLGLPEGAPLLVQALRAELVMAAEQEKYYEHEAG
                     YSDAKKKMDDEILGLTQAPDGGKLSDFLKMRDDVQKTRAAFEESVSRDEVEKESSAYS
                     NLSGIRDAALESLRRAAPGTYYAVIHYLDDIEAPFKDEKTRIEQAKQAEEMAYSVAGR
                     IGLALEPFLRPMGFDWKIGTALIGAFAAKEVFVAQMGIVYSVGKADEKSEALREQLRS
                     QYSPLVAFCIMLFCLVSAPCMATIAVTKRESNSWKWALFQLGGLTALAYVLTVMVFQA
                     GNLLGIGV"
BASE COUNT          865 a          925 c         1025 g          779 t
ORIGIN      
        1 caatagacat caaggtgaaa ggctatcatc tctccttgag gaaagaagaa gcggagggaa
       61 tcgaggttga gctcttatag ttggcggcta aagatgattt ggaggtggag gcaatgccct
      121 tggcaatggt aaaagccgga caatgtgtac atttagttgc cgtggacgcg ggtcgcggtc
      181 ttcaggccag attagccgac atgggattgg tcccgggtgt ggaactcgaa gtggtgagta
      241 atgctctaca tggccccttc atcgtagctg ttaaaggttg ccgcatggtc ctcgggcacg
      301 gaatcacgca aaaaattatg gttgcataat tttttttgca tcttgagtta ggctatccta
      361 acattatacg gaacaccaaa taaagaggag aataaaaaaa ccatgaaacc aaagattgtc
      421 attgcccttg caggcaaccc caattcgggg aagacaacta tattcaataa actcaccggc
      481 gcccgacagc atgtagggaa ttatcccggc gtcacggtcg agcgcaaaga aggctattgt
      541 cggtatggcg atatggaaat gcagatcgtc gatctgcccg ggacatacag cctgacggcc
      601 tattccaccg aggaactggt agcaagaaat tttctcatcg acgaaaagcc agatgtggtc
      661 gtggacatta tcgacgcctc gaacctcgaa aggaacctgt accttgcggt ccaactcatg
      721 gaactgggct tgcctctcgt actggccttc aatatgagcg accaagccag agcccagggc
      781 catgaattcg acatcgagaa gctttccagt tatttcggag cgagcatcgt gcagacggtc
      841 ggacacaaag gcaccggcat gaaggagctt cttgaggcca tcgttgccgc ggcaaaagac
      901 ggtgcatcgg agatgaacag gaacgatgaa aatccctcag gagccctctt gtccaaggcg
      961 acgattcata gtccattccg tcaaaggccg gtgatggact acggccggga gatcgaggag
     1021 gaaatcgccc ggctggaatc gctgattaaa ttgaaggtga caggtaagga caaagacaat
     1081 ttacgatggc tggccctgaa actactcgaa aacgacaagg cggtccgggc caggttcgac
     1141 acccctgaaa tcaacagcca actggagaaa agcgcggccc gtatcgaaaa gatgctcgga
     1201 gaacatccgg agaccgccat tgccggccgc cggtacgggt tcatctccgg cgcgtgccgg
     1261 gaggcggtac ggtccaccat tgagctgcgc cacacgatct cggacaggat cgacgctgtg
     1321 gcaacgaacc gccttctcgg cattcccata ttcatgggac tgatgtacct tgtcttccaa
     1381 ctgaccttca cgctggggga cccgcccatg aaatggatcg aggagggatt cggctggttg
     1441 ggagcccaga tcctgggttg gtggccgaaa ggttccgaaa gcgccctcaa atcgctcctg
     1501 gtggacggca tcatcggcgg cgtcggcggc gtcatcgtgt ttctgcccaa catcctgctc
     1561 ttgtttctgg ccatcgccat tttagaagat tccggctaca tggcgcgggc ggccttcatc
     1621 atggaccggg tgatgcataa gatcgggctg cacggcaaaa gcttcatccc catgctgatc
     1681 ggattcggct gctccgttcc cgcgatcatg gccacccgga cgctggaaaa ccgccgtgac
     1741 aggctcgtga cgatgctagt gaccccgctc atgagctgcg gcgcccggct gccgatctac
     1801 gcattgatta tccctgcgtt tttccctcaa gtctggcacg cccccatgct gtggctcatc
     1861 tatgtcatcg gcatcgccct cgccgtttta agcgcgaaac tccttcgcac cacgatcctc
     1921 aagggcgaat ccgtgccttt cgtgatggaa cttccaccat acaggatgcc caccctcaag
     1981 agcgtgctga tccacatgtg ggaacgggga tggctctatc tgaaaaaggc cggcaccatc
     2041 attctctgca tctcgatcct cttgtgggcc atgacaagct atccgaagaa aaccgtcttc
     2101 gatgtggatt acgaagctga cttggcgaaa tcggagggca cctacctgtc aaaggtaacg
     2161 cggttgaacg ccctcctggg gctgccggag ggggccccat tgctggtcca ggcccttcga
     2221 gccgaactcg tcatggccgc cgaacaggag aagtactacg agcatgaagc cggttacagc
     2281 gacgccaaaa agaaaatgga cgatgaaatc ctcggcctca cgcaagcgcc ggacggcggc
     2341 aaactgtcgg attttctgaa aatgagagac gatgttcaaa aaacgagagc cgccttcgaa
     2401 gagtcggtat cccgggacga ggttgaaaaa gaatcctctg catacagcaa cctgtcagga
     2461 attcgggatg ccgctctcga atctctccgg agagccgccc cggggaccta ttacgccgtg
     2521 attcactacc ttgatgacat cgaggcgccg ttcaaagatg aaaagacccg gatcgaacag
     2581 gcaaaacagg ctgaagagat ggcctattcc gttgccggcc gtatcgggct tgctctggag
     2641 ccgtttttga ggcccatggg gttcgactgg aagatcggaa ccgcgctgat cggcgccttt
     2701 gctgccaaag aggtcttcgt cgcccagatg ggcatcgttt actccgtcgg taaggccgat
     2761 gaaaaatcgg aagcgctgcg ggaacagctt cgatcccagt attctcctct tgttgccttc
     2821 tgcatcatgc tcttttgtct ggtcagcgca ccatgcatgg cgaccattgc cgttacgaag
     2881 agagagagca attcgtggaa gtgggcgctc tttcagttgg gaggtcttac ggccttggcc
     2941 tatgtactga ccgtcatggt ctttcaggcg ggcaacctgc ttggaatcgg tgtctgaaaa
     3001 gaggaatgca tgtttgaaac aataatgatc ggattgattg tcggttccgc gtttttatgg
     3061 atcagtgtct ctctgcacag gacgttgcgg ggaaagaacc actgccagtg ctgtggaaat
     3121 aaagactgct gtaccagtct tgaccggatg aagcgtgacg gtgcagagga tatcaatccg
     3181 gctactcggg agagcggcta atgaatgcac aggaagcatt gagcaacagc attcggaata
     3241 ttgagatgcg gctcaaagaa catgagcgtc ttcttgaatg cctgcgggaa ggggatgagg
     3301 ggacagttac agcctgtctg tctctggatt gccgacattc gattgtgatg agggaaactc
     3361 tcttggaggt gatagaagtt ctggaggatt cccggaaggc tttcaagtcg ccccgacttg
     3421 aggcgttgag aaaaaagttg atccggatat tggcagaaaa tatctgatcc atacgtaacc
     3481 tgatcgatcc tacggcccga ctcctggagt cgggaagtga gggcgtggtg aatcggcctg
     3541 aaaaaggccg tatccgtcac gccctttttt ttcgcgattc aacgccccgg tttg
//
LOCUS       DAOSMF010000182         3565 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_28424_length_3565_cov_16.761823, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000182 DAOSMF010000000
VERSION     DAOSMF010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28424_length_3565_cov_16.761823"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            285..1457
                     /locus_tag="PLT63_10710"
     CDS             285..1457
                     /locus_tag="PLT63_10710"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD99233.1"
                     /translation="MGLTGVRRAGKTFLFFATIQRLATQGVDRRQIVYLNFEDDRLHP
                     IAAEELDLILRCWHEVFPETAGRRKHLFLDEVQNVPGWERWVRRLCDTEDIEVYVTGS
                     SSRLLTRDLATALRGRSITLEVFPLSFRECLSFRGLEIVRYDADNESLIRHELERYLL
                     WGGFPEVVLADETLRPLILADYGPLMLYRDVVERYGVRNEKLIRELLRYAFRNTASLL
                     NVSKLHRDFVSQGFTVSRNTIFEYLGYLEDSFLIFLVPKQEASLRKQAHNPKKLHVID
                     PGLMAAFQTYPGRDLGHKLETVVFLENRRRQRDLFYYVNGSEVDLCDGDGKFFINTCW
                     RLTEPETRRREKAAMELGFARWPNAQGRLLYHEYSAVSGQEMPGAVAAWRYLLDLE"
     gene            1504..2286
                     /locus_tag="PLT63_10715"
     CDS             1504..2286
                     /locus_tag="PLT63_10715"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOD99234.1"
                     /translation="MTLELFNREVKAAVSSVEMEEDGEYQRQLALFSQSSTEKGIELV
                     RIGEIIARLDRRGSFLDIGAGGGHLTIPVSTTFDATTVVEPNAQQADYLRRRCPGFRV
                     INDAWNNVALGSERFDFILCSHVLYYIAEGRWLDTIEKMYAHLDDRGCLAIVIQSPIG
                     EVAEFFNYFTDYDVNILNLWRELIEIYGDRAVSVRYFINEIFAESLADMTAIGLFLLI
                     DRRFREREDEIRKYLAGRHRTPEGYRLKQEEILLAIRGRNAS"
     gene            complement(2612..>3565)
                     /locus_tag="PLT63_10720"
     CDS             complement(2612..>3565)
                     /locus_tag="PLT63_10720"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOD99235.1"
                     /translation="VDQAYIYSFWLVLSFPIMAYFMLGEAMLVSRGDTQTPMKTMIGG
                     NIVNLVLDPVLMFGFDMGIAGAAIATLIGWSLAVLILIRHLRRQGLAMPTWQWKSHYR
                     AEWAGMAHLSVYIFISQISSPLALALVNSILVDFGAAAVGAWTIMSRLELFSFMPLFG
                     VGSALIAFISFNTAAGLYHRIRHGLLVAVGVSAAFVLPIMAFFILWPDIAAWPFQAGG
                     DVRLLAAGAIQAAAWAHIVCPAELILFGLAQGLKKPAYYLPAVIVRLFVLRYPLAYAL
                     AGFYGLAGVFWSQPLANLGTAVVSAALIAVLLRPLPHADAV"
BASE COUNT          810 a         1029 c          983 g          743 t
ORIGIN      
        1 tcgcactcat gatgcgcgag ctttgaacga tgccctaaaa gtgttcacaa cgttaattta
       61 agtgaatgat tttaatttat tttgataata aattttttat taaatgttac aaataccagg
      121 aaatatgccc atactacctt ggggctaact aaaaatatcc ggacaaagga ctctgctttt
      181 acgagtcata aaatctcaaa tatgtgctcc gtcaattcgt tgaacggctc ctgccgcctt
      241 gcaaacccag gcaactggcc gtcccgtccg gcaccggcaa ggttgtgggg ctaacaggtg
      301 tccggagagc tgggaaaacc tttcttttct tcgcgactat ccaacgactg gcaacgcagg
      361 gtgtggaccg ccggcaaatc gtctatctga atttcgaaga tgatcggctc catcctatcg
      421 ctgccgagga actcgatttg atcctccggt gctggcatga ggtcttcccc gaaacggcag
      481 gccgccgcaa gcatcttttc ctcgatgagg tgcagaatgt ccccggctgg gagcgatggg
      541 tgcggcggct ctgcgataca gaggatatcg aggtctatgt aaccggctca tcttcgcgcc
      601 tcctcacccg ggatctggcc accgccctgc ggggccgcag cattaccttg gaagtattcc
      661 ccctgagctt ccgggagtgc ctttcctttc gaggactgga aattgtcaga tacgatgcgg
      721 acaatgagag tctcatccgt catgagctgg aacgctacct gttgtgggga ggatttcctg
      781 aagttgtcct tgcggatgaa acattgcggc cgctgatcct ggcagattac ggccccctca
      841 tgctctaccg ggatgtggtg gaaagatatg gcgtgcgcaa cgagaaattg attcgcgaac
      901 tgctgcgtta cgcctttcgc aataccgcct ccctgctcaa tgtcagcaag ctccatcggg
      961 actttgtctc tcagggattt accgtttccc gcaatacgat ctttgaatat ctcggctacc
     1021 tcgaagattc cttcctgatc tttcttgtcc cgaagcagga ggcatcgctg cggaaacagg
     1081 cccataatcc caagaagctg catgtgatcg accctggtct catggccgca tttcagacct
     1141 atccgggacg cgacctggga cacaaactcg aaaccgttgt cttccttgaa aatcgccgcc
     1201 ggcagagaga cttgttctat tacgtgaatg gaagcgaagt ggatctttgt gacggcgacg
     1261 gcaagttttt catcaacacc tgctggcgtc tcacagaacc ggaaacaagg cggcgagaaa
     1321 aagctgcgat ggagttaggc tttgcccgct ggcccaacgc ccaagggcga ctgctgtatc
     1381 atgaatattc cgccgtcagc gggcaggaaa tgcccggcgc cgttgccgca tggcggtatc
     1441 tgctggatct tgaatgatgt ttatatatcc tcgcccccgg cgggatgaag ggaggaggaa
     1501 gcgatgaccc tggagctgtt caatcgggaa gtgaaggcgg ctgtttcctc cgtggaaatg
     1561 gaggaggacg gcgaatacca gcggcaactg gccctgtttt cccaatcctc cacggagaag
     1621 gggatcgaac tggtaagaat cggcgagatc atcgcccggc ttgaccggcg cgggtcgttt
     1681 ctcgatatcg gcgccggcgg cggccatctg accatccccg tttccaccac tttcgacgcc
     1741 accacggttg tggagccgaa cgcgcagcag gccgactatc tccggcgccg ctgtccgggc
     1801 ttccgggtta tcaatgatgc ttggaacaat gtagccctcg gttcggagcg cttcgatttc
     1861 atcctctgtt cccatgtcct ttactacatt gccgagggcc gctggctcga taccatcgaa
     1921 aagatgtacg cccacctcga cgatcgcggc tgccttgcca tcgtcattca atcgcctatc
     1981 ggcgaggtcg ccgaattttt caattacttt accgattatg atgtgaacat cctgaactta
     2041 tggcgcgaac tgatcgagat atacggagac cgggccgttt cggtcagata cttcatcaac
     2101 gagatctttg ccgagagcct cgcagacatg accgccatcg gcctgttcct gctgatcgac
     2161 cgtcggttcc gggagcggga ggacgagata aggaagtacc tcgccgggcg tcaccgaacg
     2221 ccggagggat accggctcaa acaggaagag atcctcctgg caatcagggg aaggaacgct
     2281 tcctgaggcc cccttcccaa tccccgccgg cctcgttgct ttgcataagc gcggaaaaat
     2341 ggttcgcgag cggcgaatca aatgtttttc gccgttgcgt gattgaactt aacgcccggc
     2401 catgaataag ttttatacgt taactcttca gaagcgaaaa agatttctga gccgcggcga
     2461 agccattttt caaaggtctc ctcgttatcc gcgatctatt gccgtcccca ccgtcgctgc
     2521 cgtgaaacac gccgcccggc gtcctcgata aagacgctgc acatggatcc ccccggaggt
     2581 tcgtggcgcc ccgttgcgcc gtccggggat tctacacggc gtccgcatgg ggcagtggcc
     2641 gcaacaatac ggcaatcagc gctgccgaca ccaccgccgt gcccagattg gccagcggtt
     2701 gactccagaa taccccggcc aggccataaa atccagccag ggcataggcc agcgggtagc
     2761 gcaggacaaa cagccggacg ataacggccg gcagataata agccggcttt ttcaagccct
     2821 gggccaggcc aaacaggata agctccgccg ggcacacaat atgcgcccaa gccgctgcct
     2881 gtatcgcgcc ggcggcgagg aggcgcacat ctcccccggc ctgaaaaggc caggcggcaa
     2941 tatcgggcca caggatgaag aatgccatta tgggcagaac aaaggctgcc gaaacaccga
     3001 cggccaccag caggccatgc cggatacgat gataaaggcc ggcggcggta ttgaaactga
     3061 tgaaggctat gagggccgag cccaccccga agagaggcat gaagctgaac agctccagac
     3121 gggacatgat ggtccaggcg cccaccgcgg cggctccgaa atcgaccaga atagaattta
     3181 ccagggccag cgccaggggg ctgctgatct ggctgatgaa tatgtagaca gacaggtggg
     3241 ccatgcccgc ccattccgcc cggtaatggc tcttccactg ccaggttggc atagccaggc
     3301 cctgcctgcg caggtggcgg atcagaatca gcaccgccag gctccagcca atcagcgtgg
     3361 caatggccgc cccggcaatg cccatatcga agccgaacat cagcaccgga tccagcacca
     3421 ggttgacgat gttgcctccg atcatggttt tcatcggcgt ctgcgtatcg cccctgctca
     3481 ccagcatggc ttcccccaac atgaaatagg ccatgatggg aaaggacagc accagccaga
     3541 aagaataaat ataagcctga tcaac
//
LOCUS       DAOSMF010000183         3558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_28504_length_3558_cov_25.728804, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000183 DAOSMF010000000
VERSION     DAOSMF010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3558
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28504_length_3558_cov_25.728804"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(230..1915)
                     /locus_tag="PLT63_10725"
     CDS             complement(230..1915)
                     /locus_tag="PLT63_10725"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HOD99236.1"
                     /translation="MKKILLVTLLLLLAAGTLRGENYTLKGNMDAAIRYELRERIQAG
                     EGVKKMTLSFVVPQSFTSPTYSQKIDGFRAVFHPEPQERSEIRDKRGNLILQATWTRV
                     PGAVDVRLSCRADSRTYLTSLETRAPFPLRNLGPELAEYLQSTEQVQADNPRIRRLAA
                     ELAKGVKTEYDAVLRIVSWVVDHVRYVNPPVRYDALYSLETGKGNCQNYSHLSAALLR
                     SLGIPTRIVVGVTLNQPYDINWKQGVLTFKMGQGRHSWIEAWFPDLGWVPFDPQNSIL
                     FVSNRFIRIETGLDNNEAKNDGLMRWSQVRGTPNPSLEEIINTDFASDQVKIQGIRET
                     YGPKNLLLTPKVLAQFQQVAVPPPPPPPPPPKDPSRLRYNVPFLYGNLDYPENVDFAF
                     PRGVKSSGAEQFETQNNFLVETAEYVTSQRTQYAQIFVLKKPVKLKQVGLALHNFGGD
                     GWLWIDLFKDNKGKPGALLATSEMINLDQISPRPGYRWEDFDFGRENPELMPGSYWIA
                     LGFTGSPIVNWFYTYGKPAGPVDGTRYRGVFDRDWSGALSYEFNYRIRGFTVQ"
     gene            2016..>3558
                     /locus_tag="PLT63_10730"
     CDS             2016..>3558
                     /locus_tag="PLT63_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOD99237.1"
                     /translation="MKISRVEEMRAMDRRATEELGISEEILMENAGQAAVHVLAKERG
                     IRGKRYVILCGVGNNGGDGFVVARKILSLGGHPRVYLLGDPGRCRGAAAVNLAILSRL
                     SVEVSRPETATSLRREIVHADGIVDAVFGTGLDREVRGLYREAIELVNVRRSWVLSLD
                     IPSGVNGETGEIMGAAIRADATVTFGLPKPGNVLPPGCDQGGRLYVTHISFPPFLYNR
                     DDLKIQVNDGPPLPVRGREVHKGAMGDALFIAGARTYLGAPFFAAMSFLKAGGGYARL
                     AAPAGAIPAIAAQGKELVFVPLRETPEGAMAADNFDELCAVAEKVDMVIIGPGVSLNP
                     ATQNLVRRLVPAVAKPLLIDGDGLTAVAGQRELVRGRSFPTVLTPHAGEMARLTGLTA
                     AEIKRHRIDVLQETCAAWGAHIVLKGARSLIGGPDGRVHVNMTGNPGMATAGSGDVLT
                     GTIAAMFGLGLPWPECIRKGVYIHGRAGDLAAAERGEDGITAGDILDFLPEAVREDRR
                     HGEGGA"
BASE COUNT          673 a         1022 c         1168 g          695 t
ORIGIN      
        1 ccccggcgaa gaggagcgag gcgagaccgg cgcgggcggc gtagagaccg gcggtatagc
       61 ccgccggtcc tccaccgacg ataataacgt cataaatctt tttgaaatcc gtgttgacgg
      121 ccatggaagc tgctgattct cctttcccaa gcgaatggca tttgacggtt aagcatctga
      181 attccaaatc ttatagaata aataagcgga agagtcaaat ttcatgacct cactgaaccg
      241 taaaccccct aatccggtaa ttgaattcat agctcaaggc gccgctccag tcccggtcga
      301 aaacaccacg gtagcgggtg ccgtccacgg ggcctgcggg cttgccgtag gtatagaacc
      361 agttgactat ggggctgccg gtgaagccca gggcgatcca gtaagaaccg ggcattagtt
      421 ccggattctc cctgccgaaa tcgaaatctt cccagcggta gcccggccgc ggagatattt
      481 gatcgagatt gatcatctcg ctggtagcga gcagcgcccc cggtttgccc ttgttgtcct
      541 tgaaaagatc gatccacagc cagccgtcgc cgccgaaatt gtgcagggcc agtcccacct
      601 gcttcagctt caccggtttt ttcaagacaa atatctgggc gtactgagtg cgctgggaag
      661 taacatattc cgccgtttcg acaagaaaat tgttctgggt ttcgaattgt tccgctccgg
      721 aggatttcac cccccggggg aaggcgaaat cgacattctc cgggtaatcg aggttgccgt
      781 aaaggaaggg gacattataa cggagtcggc ttggatcttt gggaggcggc gggggaggcg
      841 gcgggggggg gacggcaacc tgctggaact gggccaggac cttgggagtc agcagcaggt
      901 ttttcggacc gtaggtctcc cgaatcccct ggatcttgac ttgatcggag gcgaaatcgg
      961 tgttgatgat ctcctcgagg gacggattcg gggttccccg gacctgcgac caacgcatga
     1021 ggccgtcgtt ttttgcctcg ttgttgtcca agccggtttc gatgcggatg aaccggttgg
     1081 acacaaagag aatggaattc tggggatcga agggcaccca gcccagatcg gggaaccagg
     1141 cctcgatcca ggagtgacgc ccctgtccca tcttgaaggt cagcaccccc tgtttccagt
     1201 tgatgtcgta gggctggttg agggttaccc cgacgacgat gcgggtggga atccccaggg
     1261 aacgtaacag ggcggcgctg agatgggagt agttctggca gttgcctttt cccgtttcca
     1321 gggaatacag ggcgtcgtaa cgcaccggcg ggttgacgta gcggacatgg tccaccaccc
     1381 aggatacgat ccgcagtacg gcgtcatact ccgtcttgac ccctttggcc agttcggcgg
     1441 cgagtcggcg aatccgggga ttgtcagcct ggacctgttc cgtggactgg agatattcag
     1501 ccagctcggg gccgagattc cggaggggaa agggagcccg ggtttccaga gacgtcagat
     1561 aggtccggct gtcggcccgg caggaaagcc ggacatccac ggcgccggga acgcgggtcc
     1621 aggtcgcctg taagatcagg ttgccccgtt tgtccctgat ctccgaccgc tcctggggct
     1681 cgggatggaa aaccgcccga aaaccgtcta tcttttggct gtaggtggga gaggtgaaac
     1741 tctgggggac gacgaagctc agagtcattt ttttcactcc ctccccggcc tggatccgct
     1801 ctcggagctc atagcggatg gcggcgtcca tattgccctt cagggtatag ttttcgcccc
     1861 ggagggtacc ggccgccagg aggaggagca acgtaacaag cagtatcttt ttcattcttt
     1921 ttctcctgat cgagagggat catccctgcc cttgacgggg ccattgtaac aaaatataat
     1981 acaaaaaagt agacttttca tgagaggaag gcggcatgaa gataagcagg gtggaggaaa
     2041 tgcgggccat ggatcgtcgg gccacggagg aattggggat atccgaggag attctcatgg
     2101 agaacgccgg acaggcggcg gtccatgtcc tggcgaagga gcggggtatc cgggggaagc
     2161 gctacgtgat cctctgcggg gtcgggaaca acggcggcga cggatttgtc gtcgcccgga
     2221 agattctttc cctgggtggg catcctcggg tgtacctcct gggggacccc ggccgatgcc
     2281 ggggagcggc tgcggtaaat ctggcgatcc tttcccggct ttccgtggaa gtgtcgcgac
     2341 cggaaacggc gacgtccctc cggcgggaga tcgtccatgc cgacgggatc gtggatgccg
     2401 ttttcggcac cggcctggac cgggaggtcc ggggtctgta ccgggaggca atcgaactgg
     2461 tgaatgtccg ccgttcctgg gttctcagcc tggacatccc ctcgggtgtc aacggggaga
     2521 ccggagagat catgggcgcc gccatccggg ccgacgccac ggtgaccttc gggctcccca
     2581 agcccggcaa tgtcctcccc ccgggctgtg atcagggggg gcggctctat gtgacccata
     2641 tctcctttcc ccccttcctt tacaaccgcg acgacctgaa gatccaggtc aacgatggcc
     2701 cgcccctgcc ggtccggggg cgggaggtcc ataagggtgc catgggggac gccctcttta
     2761 tcgccggggc gagaacctat ctgggcgccc ccttctttgc cgccatgtcc ttcctgaagg
     2821 ccgggggggg gtacgcccgc ctggccgcac cggcgggtgc aattcccgcc attgccgcgc
     2881 agggcaagga actcgttttc gtgcccctcc gggagacgcc cgaaggggcc atggcggcgg
     2941 acaacttcga cgaactttgt gccgtggcgg aaaaggtgga catggtcatc atcggtcccg
     3001 gggtctccct gaatccggcg acgcagaatc tcgtgcgccg gctggtgccg gccgtcgcca
     3061 agccattgct catcgacggc gacggcctca cggcggtggc gggacaacgg gaactggtcc
     3121 ggggtcggtc gttccccacg gtcctcaccc ctcatgccgg ggaaatggcg cgcctcaccg
     3181 gcctcacggc ggcggagatc aagcggcacc ggatcgatgt cctgcaggag acctgcgccg
     3241 cctggggcgc ccatatcgtc ctcaaggggg cccgttccct catcggtggc cccgacggcc
     3301 gggtccatgt gaacatgacg ggcaatcccg gcatggctac cgccggttcc ggagatgtcc
     3361 tgacgggaac catcgccgcc atgttcggat tgggcctgcc ctggccggaa tgcatccgta
     3421 agggtgtcta tatccacggc cgggccggtg acctggcggc ggcggaacgg ggcgaggacg
     3481 gcataaccgc cggagacatc ctcgactttc tgcctgaggc cgttagggag gatcgccgac
     3541 acggggaagg cggggcgg
//
LOCUS       DAOSMF010000184         3541 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_28677_length_3541_cov_24.556799, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000184 DAOSMF010000000
VERSION     DAOSMF010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28677_length_3541_cov_24.556799"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="PLT63_10735"
     CDS             complement(<1..686)
                     /locus_tag="PLT63_10735"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOD99238.1"
                     /translation="MKELSRKRILVVEDDPHISEGLRLNLTLKGHEVMIAADGMAALR
                     EWRNWRPQLIVLDIMLPGIDGLAVLRHIRIEDEKLPILILSAKSEPEYRVKGLSYGVD
                     DYLSKPFNLDEFLLRVERLLTRAAWNHEAARESRSDRGSEGIAKLGATYRFGENMVHF
                     DTFTAHGRCGEVKLTEQEMRLLKLFITNRGRPLSRSELLEMGWGYTGATPTRTVDNFI
                     VRFRKYFETNP"
     gene            complement(683..1657)
                     /locus_tag="PLT63_10740"
     CDS             complement(683..1657)
                     /locus_tag="PLT63_10740"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOD99239.1"
                     /translation="MKRVKWFVHPVVIFILSIAALGASLFLYIYWYLEVSTGLQKIIM
                     KYSLDPAQFFEIKTWVVIMVLSILVGLILTGVFTIFVYNEKTQRLYRMQRNFINSFTH
                     ELKTPVASLRLYLDTFSRYELPREDQLRYLDFMSHDVNRLLANINRILDLARIETKSY
                     QKGRECIDIVKMVEEFCEQNADLFRGCDIAIHNPSRERPCYWINRPLFEMLLMNLFTN
                     AVKYNQSDRPRIDVAFVTRKKGLDIRITDNGMGIEKGETKKIFRKFYQVGHTEDMTAK
                     GTGLGLYLVAHVAQIHQARVTAESPGPGQGSTFVLKMPPAVRSVEAAA"
     gene            1833..>3541
                     /locus_tag="PLT63_10745"
     CDS             1833..>3541
                     /locus_tag="PLT63_10745"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HOD99240.1"
                     /translation="MAGHKVPVHPLRTNANVLLASVFGPYAQDDQYGSRANNPMELYH
                     NQVTRLQGGFSLRMFHRSFGLLMIQANIGAPCTVLDFPRREDFIRELQNNDYDIIGIG
                     SILTNIGKVREMCDLIRRYQPGATIVVGGHIAALEELHRRIDADYVVRGDGIRWFRRF
                     LGQDEEAPINHPLVLSGFGARMIGIELPHRKGHTAAILIPSVGCPMGCNFCSTSAFFG
                     GKGNFVNFYETGDELFSVMCRMEEKLSVRSFFVLDENFLLHRRRALRLLELMEEHGKS
                     WSLDVFSSARVIQSYPIEQLVRLGVTWIWMGLEGKESAYAKLNGVDTRRLIKILQSHG
                     IRVLGSSIIGLEEHRPEDMEEIISYAVAHDTDFHQFMLYTPNPGTPLYRRHLEDGDIL
                     PETELPLADRHGQYRFNYRHRHITGGQEEEILLKAFRRDFEENGPSLYRLIRTMLQGW
                     RRYGNHPDPAVRDRYTWDTAPLRTTYTGAVWAMKKWYEKNPALAAKMDELLRDLYRTY
                     GLRTRLLAPLIGMYALTRLRREERRLNRGWAYEPRSFVNKNEAALARKKSRRFRVEIA
                     VPEA"
BASE COUNT          691 a         1092 c         1020 g          738 t
ORIGIN      
        1 gggttggtct cgaaatactt gcggaagcgg acgatgaagt tgtccaccgt ccgggtgggg
       61 gtggcgccgg tatatcccca gcccatctcc aggagctcgc tccggctcag gggtcggccc
      121 cgattggtga tgaagagctt gaggaggcgc atctcctgct ccgtgagttt gacctcgccg
      181 caccgtccgt gggcggtgaa ggtgtcgaaa tggaccatgt tctcgccgaa gcggtaggtc
      241 gctcccagct tggcgatgcc ttcgcttccc cggtccgacc ggctttcccg tgccgcttca
      301 tgattccacg ccgcccgggt caggagccgc tccacccgca ggaggaactc gtcgaggtta
      361 aacggtttgg aaaggtaatc gtcaacgccg taggacagcc ccttcacgcg gtattccggt
      421 tcgctcttcg cggagaggat gagaatcggc agcttttcgt cttcgatgcg aatatggcgc
      481 aggacggcca gcccatcgat gccggggagc atgatgtcca ggacgataag ctggggccgc
      541 cagttccgcc actcccggag cgccgccatg ccgtcggcgg cgatcatcac ctcgtggccc
      601 ttgagggtca gattcagccg gaggccctcc gaaatatgcg gatcgtcctc cacgacgagg
      661 atccttttcc tggacagttc cttcatgccg ctgcctccac gctacggacc gccgggggca
      721 ttttcaggac gaaggtcgaa ccctggcccg ggcccggact ctccgcggtg acccgggcct
      781 gatggatctg ggcgacatgc gcgacgagat aaagtcccag tcccgtgccc ttcgccgtca
      841 tgtcctcggt gtgccccacc tggtagaact tgcggaagat ctttttcgtt tccccctttt
      901 cgatccccat gccgttgtcg gtaatgcgga tgtcgagacc ctttttacgg gtcacgaagg
      961 cgacgtcgat ccggggccgg tcggattgat tgtatttgac cgcgttggtg aacaggttca
     1021 tgagcagcat ctcgaacagt ggcctgttga tccagtagca gggccgctcc cgggacgggt
     1081 tgtgaatggc gatgtcgcac ccccgaaaca aatcggcatt ttgttcacaa aattcttcaa
     1141 ccattttgac aatgtcgata cattcccgcc ccttttggta acttttcgtc tcgatccggg
     1201 ccagatcgag gatgcggttg atatttgcca gcaggcgatt gacgtcgtgg ctcatgaagt
     1261 ccaggtagcg gagttggtct tcccggggca gttcgtagcg ggagaaggtg tcgagataga
     1321 ggcggaggga cgccacgggg gttttcagct cgtgggtgaa gctgttgatg aagttccgct
     1381 gcatccggta gaggcgctgg gtcttctcgt tatagacgaa aattgtgaat acccccgtca
     1441 ggatgaggcc gaccagtatg gagagcacca tgatcaccac ccaggtcttg atttcgaaaa
     1501 attgggccgg atcgagactg tatttcatga ttatcttttg taaaccggta cttacctcca
     1561 gataccaata gatgtacaaa aaaagggacg cccccagcgc cgcgatggac aggatgaaga
     1621 tcaccaccgg atgtacaaac cattttaccc gtttcataag cgtggagatt agctacacca
     1681 gcgccgtgaa actgtcaatg ccggatgatc gccgtcgctg ggaccaattg taaaagtaat
     1741 gtaaaatcaa caattagtgc ttttctttcc cctcctaatt ccgtattagg atcaccatcg
     1801 cacgaaaaaa tccatagtta aggagatcta tcatggccgg tcataaagtc cccgtccatc
     1861 ccctgcgcac taatgccaac gtcctcctcg ccagcgtttt cggtccctac gcccaagacg
     1921 accagtatgg gagccgcgcg aacaatccga tggagctgta tcacaatcag gtaacccggc
     1981 ttcagggggg cttttccctg cggatgttcc accggtcctt cgggctcctc atgattcagg
     2041 ccaatatcgg ggccccctgc acggtcctgg attttccccg ccgggaggat ttcatccggg
     2101 agctgcaaaa taacgactac gacatcatcg ggatcggttc catccttacc aatatcggca
     2161 aggtgcggga gatgtgcgac ctgatccgcc gctatcaacc cggggcgacc attgtcgtcg
     2221 ggggtcacat cgccgccctg gaggaactcc atcgccgcat cgacgccgac tatgtagtcc
     2281 ggggggacgg tatccgctgg ttccggcggt ttctcgggca ggacgaagag gcgccgatca
     2341 accatcccct ggtcctttcc ggcttcggtg cccgcatgat tgggatcgaa ctcccccacc
     2401 ggaaggggca caccgccgcc atcctgatcc cctcggtggg ctgtccgatg ggatgcaatt
     2461 tctgctcgac ctcggccttc tttgggggca agggaaattt cgtcaacttc tatgaaaccg
     2521 gcgacgagct cttttccgtc atgtgccgca tggaagagaa gctctccgtc cgctccttct
     2581 ttgtcctgga cgaaaacttc ctcctccacc gccgccgggc cctccgcctg ctggaactca
     2641 tggaggaaca cggcaagtct tggtccctcg atgtcttcag ctccgcccgg gtcatccaat
     2701 cttatcccat cgagcaactg gtgcgcctgg gcgtcacctg gatctggatg ggcctggagg
     2761 gaaaggaaag cgcctacgcc aagctcaacg gcgtcgacac ccgccgcctg atcaagatac
     2821 tccagtccca cggcatccgc gtgctggggt cttccatcat cggcctggag gagcaccgtc
     2881 ccgaggacat ggaggagatc atctcctacg ccgtcgccca cgacacggat tttcatcagt
     2941 tcatgctcta cacccccaat cccggcacgc ccctctaccg tcggcacctg gaggatgggg
     3001 atatccttcc cgaaacggag ctgcccctgg ccgaccggca cgggcagtac cggttcaact
     3061 atcgccaccg ccacatcacc ggcgggcagg aagaggagat cctactcaag gcgttccgcc
     3121 gggatttcga ggaaaacggt cccagcctct accgcctgat ccgcaccatg ctccagggtt
     3181 ggcggcgtta cggtaatcac cccgatcccg ccgtccggga ccgctatacc tgggacaccg
     3241 cgccgttgcg caccacctat acgggggcgg tctgggccat gaagaagtgg tatgagaaga
     3301 accctgccct ggcggccaaa atggacgaac tcctgcgaga tctctaccgc acctacgggt
     3361 tgcggacccg cctcctggcg ccccttatcg gcatgtacgc cctgacccgg ctccgccggg
     3421 aggaacggcg cctgaaccgg ggctgggcgt atgaaccgcg gtcgttcgtc aacaagaacg
     3481 aggcggccct ggcccggaag aaatcccgcc gcttccgcgt cgagatcgcc gtgccggagg
     3541 c
//
LOCUS       DAOSMF010000185         3527 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_28810_length_3527_cov_24.587558, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000185 DAOSMF010000000
VERSION     DAOSMF010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3527
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28810_length_3527_cov_24.587558"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2946
                     /locus_tag="PLT63_10750"
     CDS             <1..2946
                     /locus_tag="PLT63_10750"
                     /inference="COORDINATES: protein motif:HMM:NF019966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOD99241.1"
                     /translation="PEIRITAYELEHGATGAQVLHLHCADRENLFAVAFRTPPHDSTG
                     LPHILEHSVLAGSERYPVKDAFNELLRGSLQTFINAFTYPDKTIYPVASQVKADFYNL
                     ARVYADLVFRPRLLPETFRQEGHHLTWTATPTGGRELDISGIVYNEMKGAYSSPDSLM
                     YKALQENLFPDTVYRFDSGGNPADIPHLTYESFRAFHRSFYSPSNARIFLYGDIPVTE
                     HLPFLQEILAGFPRIDIDSTIPAQRRWEHPRTVRGVFPIGRGEDPRGKVNVNMAWLLT
                     ENTNEETVILLQVVSGVLVGSAAGPLRKALIDSGLGQDLSPVTGFERDYRQAVFAVGI
                     RGTDADRAEAIASLILDTLDRIVREGVDRELIEGTLHQIEIQGREINRKNYPYGIILM
                     TRVFHTWMYGGDPLYSLNFPALIAMIRRRWQEDPELFAALIREWLLENPHRLLSVMEP
                     DPNCLGKEDEARKSALGARAAALTEADQTRIETELAALKNFQTEPDTPEAVATLPRLK
                     TGDIDRKVETIPTEYAALGGTAVLRHEIFTNGIAYLDLAFDVAAVPEAQQPCLPLLGK
                     IVTQMGAAGRDYEEMAKLIALNTGGIRSSASTGFVMDLGGGTLPEGRGVWQRLIFRLK
                     ALHRHLPAAMDILADLLSAGDLSDHRRLRDLILERKNRFQASIIPSGHLFARRLAGAA
                     LSLPAWRDEQWHGKSQFDFLREASSEHNREDFAVELHELKKRTFTKAGLMVNMTADAE
                     GLRIMSDHLPALLDRLAGETPGAATAPSPREPRDRGVVIPAQVSYVARALPAPPHGHG
                     DTAALMVASRFLSNGYLYKQIRIQGGAYGGMSSYDPQTGVFALMSYRDPHIIRTLKVY
                     DEIAAALAPEHVAPEELDKAIIGAIGAIDRPMDPSGKGLAALVRHLSSLTDAYRQSLR
                     EEILGLTGEGFRAATGRFLAAAAAARTADAVFGAADRLREANEQRPGEELPLEEIMGS
                     GPTGNPEHS"
     gene            3054..>3527
                     /locus_tag="PLT63_10755"
     CDS             3054..>3527
                     /locus_tag="PLT63_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99242.1"
                     /translation="MKWRLHSSRGQLLPAAVCRSAGFDRRTIGGFGFGLRRLGRSNPS
                     PKNIRSIAAAAVVLAIVLTFGGCAGGRYGNIFPDAQTTQDFDSFRIDPGMNYYYSGPD
                     LFPNALIGLKKEYVLDSDLWKPISPTPASFRDMILHMQEKARDYREFQHGFLMYDD"
BASE COUNT          660 a         1247 c         1032 g          588 t
ORIGIN      
        1 cccgaaatcc gcatcaccgc ctatgagctg gagcatggcg ccaccggggc gcaggtcctc
       61 cacctccact gcgccgaccg ggagaatctc ttcgccgtcg ccttccggac gcccccccac
      121 gattccacgg ggctgcctca tatcctggag cactccgtcc tcgccggctc ggagcgttat
      181 cccgtcaagg acgccttcaa cgaactcctc cggggcagcc tgcagacctt catcaacgcg
      241 ttcacctatc ccgacaagac catttacccc gtcgccagcc aggtcaaggc ggacttctac
      301 aacctggccc gggtctatgc cgatctggtc ttccggcccc gcctccttcc cgagaccttc
      361 cgccaggaag gccatcacct cacatggacg gccaccccca ccggcggtcg ggagctggac
      421 atctcaggaa tcgtttacaa tgagatgaag ggggcctatt catccccgga cagcctcatg
      481 tacaaggcgc tgcaggagaa cctctttccc gatacggtct atcgcttcga ctccggaggc
      541 aatccggcgg atatccccca tttgacctac gagtcgttcc gtgctttcca tcgctccttc
      601 tactccccca gcaacgcccg catcttcctg tacggcgaca tccccgtcac ggagcatcta
      661 ccctttcttc aggagatcct cgccggcttt ccgcggatcg acatcgactc caccatcccc
      721 gcccaacggc gctgggagca tccccgcacc gtccggggcg tcttccccat cggccgggga
      781 gaggatcccc ggggaaaggt gaacgtcaat atggcctggc tcctcacgga aaacaccaac
      841 gaagagacgg tgatcctgct tcaggtcgta agcggggtcc tcgtgggcag tgcggcgggg
      901 cccctgcgca aggccctcat cgattcgggc ctgggccagg acctctcgcc ggtcacgggt
      961 ttcgaacggg actaccgcca ggcggtattc gccgtgggga tccggggcac cgacgccgac
     1021 cgggccgagg ccatcgcgtc cctgatcctg gacaccctgg accggatcgt ccgggagggg
     1081 gtggaccggg agctcatcga agggaccctc caccagatcg agatccaggg ccgggaaatc
     1141 aaccgcaaga actaccccta cggcatcatc ctcatgaccc gggtgttcca cacctggatg
     1201 tacggcggcg accccctgta cagtctgaat ttccccgccc tcatcgccat gatccgccgc
     1261 cgctggcagg aagatccgga actcttcgcc gccctgattc gggagtggct cctggagaat
     1321 ccccaccggc tcctgagtgt aatggaaccg gacccgaact gtctgggaaa agaggatgag
     1381 gcccgaaaaa gcgccctggg cgcccgagcc gccgccctga cggaagccga tcagacccgg
     1441 atcgaaacgg aactcgccgc cctgaagaac ttccagacgg aaccggatac cccggaggcc
     1501 gtggcgaccc tgccccggct gaagaccggg gacatcgaca ggaaggtcga gaccattccc
     1561 accgaatatg ccgccctggg aggcaccgcc gtcctcagac acgaaatctt caccaacggc
     1621 atcgcctatc tcgatctggc cttcgacgtc gccgccgttc ccgaggcaca gcagccctgc
     1681 ctccctctcc tgggaaagat cgtcacccag atgggcgccg ccggccggga ttacgaggag
     1741 atggccaagc tcatcgccct caataccggc gggatccgct cctccgcaag caccggtttc
     1801 gtcatggacc tcgggggcgg cacattgccg gagggacgcg gtgtctggca gcggctcatc
     1861 ttccgcctca aggccctgca ccgccacctc cccgcggcca tggacatcct ggcggatctg
     1921 ctgagcgccg gagatttgtc ggatcaccgg cggcttcggg accttatcct ggaaaggaaa
     1981 aaccgtttcc aggcctctat cataccctcc ggacacctct tcgcccggcg gctggcgggg
     2041 gcggcgctct ccctccccgc ctggcgggac gagcagtggc acggcaagtc acagttcgac
     2101 tttctccggg aagcaagcag cgaacacaac cgggaggact tcgcggtgga gctgcacgaa
     2161 ctaaagaaac ggacctttac gaaagcgggg ctgatggtga acatgaccgc cgacgccgag
     2221 gggttgcgga tcatgtcgga tcatctgccg gccctcctgg accggctcgc cggggaaact
     2281 cccggcgccg ccaccgcgcc gtccccgcgg gagccccgcg accggggcgt cgtcatcccg
     2341 gcccaggttt cctacgtagc ccgggcgctg cccgccccgc cccacggcca cggcgacacc
     2401 gcggccctca tggtggcctc ccgcttcctg tcgaacggct atctctacaa gcagatccgg
     2461 atccagggcg gcgcctacgg cggcatgtcg tcctacgacc cccagacggg ggtcttcgcc
     2521 ctcatgtctt accgtgaccc ccacatcatc aggaccctga aggtttacga cgaaatagcc
     2581 gcggctctgg caccggaaca cgtcgcaccg gaggagctgg acaaggccat catcggcgcc
     2641 atcggcgcca tcgatcggcc catggacccc tccgggaagg gcctggccgc cctggtccgc
     2701 cacctgtcct ccctgaccga cgcctaccgg cagtccctcc gggaagagat cctgggcctg
     2761 accggcgaag gattccgagc ggccaccggc cgttttctgg cggcggccgc cgcggcccgg
     2821 accgccgatg ccgtgttcgg cgccgcggac cgcctccggg aggccaacga acagcgtccg
     2881 ggggaagagc tgcccctgga ggagattatg gggtcaggcc ccaccgggaa tccagaacat
     2941 tcttgacgat gctggcttcc cgctttcgcg ggaatgacga tatggaggct ggaacgcagt
     3001 cattcaaagg cctcaggtct tggactatgt cgggaaaaga aaggagaaat gccatgaagt
     3061 ggaggctgca cagttcgaga ggacaacttt taccggccgc cgtttgccgt tctgccgggt
     3121 ttgaccggag aaccataggg ggcttcggct tcggtttgcg ccggctggga aggagtaatc
     3181 ccagcccgaa aaatatccgt tccatcgccg ccgccgcggt cgtcctggcg attgtcctga
     3241 ccttcggggg ctgcgccggc ggccgctacg gaaacatctt tcccgacgct caaaccaccc
     3301 aggacttcga ttccttccgg atcgaccccg ggatgaacta ctattacagc ggccccgacc
     3361 tcttccccaa cgccctcatc ggcctgaaaa aagagtacgt cctggacagt gacctctgga
     3421 agcccatcag ccccaccccg gcaagtttcc gggacatgat cctccacatg caggaaaaag
     3481 cgagggatta ccgtgagttc cagcatggat tcctgatgta cgacgac
//
LOCUS       DAOSMF010000186         3507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_29049_length_3507_cov_16.438586, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000186 DAOSMF010000000
VERSION     DAOSMF010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29049_length_3507_cov_16.438586"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="PLT63_10760"
     CDS             complement(<1..392)
                     /locus_tag="PLT63_10760"
                     /inference="COORDINATES: protein motif:HMM:NF017658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HOD99243.1"
                     /translation="MKEEKDCRPYRNKGKINRISSCRRKLFCLIALFFFSANLAFANP
                     YGPQVVNGNVTFQNQGNTLTVTNSPNSIINWQGFSIGANELTRFVQQHSLSAVLNRVV
                     GQDPSAILGALQSNGRVFLINPNGILFGP"
     gene            complement(419..2017)
                     /locus_tag="PLT63_10765"
     CDS             complement(419..2017)
                     /locus_tag="PLT63_10765"
                     /inference="COORDINATES: protein motif:HMM:NF015803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HOD99244.1"
                     /translation="MIVRCFLILLLLEMPFFVLPTANYAAELQGEGTPRFEIRSYRVE
                     GNSLLTQGAIDKILTPYTGKDSDFGTVQEALEALEQAYHRRGYTAIQVFLPEQALERG
                     EVMFRVMEARMGAVKVEGNRYFSQGNIRKSLPALREGVPPNIDRISTNLRTANENPAK
                     KVNLQMQGGEKENEVNAVLKVTDERPWKIAFMGDNSGTRQTGKARTGVLVQHANVADL
                     DHLFTAMYTTSPDHIKEVSIYGASYHIPIYAWSDSIDLFGGYSDVDSGTLPMGTANLA
                     VSGKGTVAGGRYNQTLTRLGRYEHKLIYGVDFRDYQNNVNLAGIPLDTSVRVTPLSLS
                     YGGSMQFTGAETGFNVGLFRNIPVCTGGKEADFSQVRTNAPADYTIFRYGGNLGYVLP
                     QDWQFRLVFNGQYTDSALIPGEQFGLGGATSVRGFDEREVANDRGIFGSAELYTPNLT
                     QWLSLPKSLCRLLAFYDIGQVSGVNVQPGDEGRMTLTSAGVGIRLDIMKILTMALDYG
                     YVLNPGLTGDRGNSRFHIRAVLTF"
     gene            2732..3340
                     /locus_tag="PLT63_10770"
     CDS             2732..3340
                     /locus_tag="PLT63_10770"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOD99245.1"
                     /translation="MVMKTLTNPFFVEMEKGARKAEQELNINLIVKTGAKETSIEQQI
                     AIVDELIRNRVEAIVIAPASSVEIIPILKKAQDTKIVIVNIDNQVDPELSRKMGLTEV
                     PFISVDNEKGAYLAAGTSATGSRNRPRSPFSKESGARRAPNSAEQRKADAVKAFGENR
                     NIRIVASETANWRIDEAFDVTAKIFNRVASCRCLSPPKPARF"
BASE COUNT          818 a          984 c          951 g          754 t
ORIGIN      
        1 ggtccgaaga ggatgccgtt gggattgatg aggaagaccc ggccgttgga ctggagggcg
       61 ccgaggatcg ccgaggggtc ctggccgacg acgcggttca gaacggcgct gaggctgtgt
      121 tgttggacga agcgggtcag ttcgttcgcg ccgatggaga atccctgcca gttgatgatg
      181 ctgttgggac tgttggtcac cgtcagggtg ttgccctggt tctggaaggt gacattcccg
      241 ttaaccacct gaggcccgta aggattggcg aaggcgagat tggcgctgaa gaagaacagg
      301 gcaatgaggc agaaaagctt gcggcggcag gaactgattc tgtttatttt tcccttgtta
      361 cgataaggac ggcaatcttt ttcttccttc atgatcctat cctcattgcg gtcgtttctt
      421 aaaaggtcag caccgcccga atgtgaaagc ggctgtttcc ccggtccccc gtcaagcccg
      481 gattaagcac atagccataa tccagggcca tggtgagaat cttcatgata tccaaccgga
      541 tgccgacacc ggcgctcgtc agggtcatcc gtccctcatc ccccggctgg acgttgactc
      601 cggatacctg gccgatgtcg taaaatgcca acaggcggca gaggctcttc gggagagaca
      661 gccactgggt aaggttcggc gtgtatagtt ccgcgcttcc gaaaatcccc cggtcgttgg
      721 cgacttcccg ttcatcgaag ccccggacgg aagtggcccc gcccaatccg aactgctccc
      781 cggggatgag ggccgaatcg gtgtattgcc cattgaaaac gagacggaac tgccagtcct
      841 ggggcaggac gtaaccgaga ttgcccccgt agcggaaaat cgtgtaatcc gccggggcgt
      901 tggtccgcac ctggctgaaa tccgcttcct tgccgccggt gcagacaggt atgttccgga
      961 acaaccccac attgaaaccc gtttccgcac cggtgaactg catggagccg ccgtagctga
     1021 ggctcagggg agtgacgcgg acacttgtat ccagaggaat cccggccaga ttgacgttgt
     1081 tttgataatc ccggaaatcg actccataga tcagtttatg ttcatagcgg ccgaggcgcg
     1141 tcagggtctg gttgtaacgc ccccccgcca ccgttccctt gccgctcacc gccagattgg
     1201 ccgtgcccat gggtagggtt cccgaatcca catccgaata gccgccgaaa agatcgatgg
     1261 aatcggacca ggcgtagatg ggaatatggt agctggcgcc gtagatgctc acctccttga
     1321 tatgatcggg agaggtagtg tacatggcgg tgaataaatg gtccaggtca gccacgttgg
     1381 catgctgcac gagcaccccc gtccgggctt tccccgtttg acgggttccc gaattatctc
     1441 ccatgaaggc gattttccat ggtctctcat ccgtcacctt gaggacggcg ttgacctcgt
     1501 tttccttctc ccctccctgc atctggaggt tgaccttttt ggcgggattt tcgttggccg
     1561 tgcgcagatt cgtagagatg cggtcgatat tgggaggcac gccttcccgc agggccggga
     1621 gactcttccg gatattcccc tggctgaaat aacggtttcc ttccaccttt accgctccca
     1681 ttcgcgcctc catcacccga aacatcacct cgccccgctc cagggcctgc tccggaagga
     1741 acacctggat cgccgtgtag ccacggcggt gataggcctg ctccagggcc tccagggcct
     1801 cctgcaccgt gccaaaatca ctgtccttcc ccgtgtaagg ggtcaggatc ttgtcgatgg
     1861 cgccctgggt gaggaggctg tttccctcca cccggtagct acggatctca aagcggggcg
     1921 tcccctctcc ttggagctca gcggcgtaat ttgccgtcgg gagcacaaaa aagggcattt
     1981 ccaacagcag caagattaaa aagcagcgta caatcaaggg aatattaatc ttccgaaccc
     2041 cccgtggaga tgttttaaca ccgatatgaa aaaatcgacc gcccggataa gatgtgatgc
     2101 gattatgaaa cccaattaat atatgaagat gaggtgtcga tgtaccaaag cgagattttg
     2161 cccgccaaca ttttatacaa gagctttgaa aaactccctt gcagtctcca catcgtcata
     2221 ccggcgacag cccgtagcca tcgctttcaa caactttccg gattccgggt cgggcccgcc
     2281 ccgtgccacg atacggggcc cgtaatgaca gaaggaggcg tttttcaaag gtctcatgca
     2341 tggcatcaca agtcaacgcc atgcagccat tcccctatgt gatcgggtaa tccataaaac
     2401 actggattat ccggtcaagc cggacaatga caaatacctg ttcaacatgg agccatgtat
     2461 ggtaataaga atgatgacat agtatcttgc tgttttaaaa aaatgcaaga aaaaaagcga
     2521 tgaaacggat aaacactttc ttaaaaaaca acatacattt tcattccctt ttgtgacggg
     2581 acaccgtcac ggacgttggt cccgtcaatt ctccttgaca ttacggggct cctcctcata
     2641 ttctttctta gagctttgaa aaactccctt accccgggac aggcaaacgg cgacggataa
     2701 acaatcctcg ccagccaaaa agcagatcgc cctggtgatg aaaaccctca ccaatccttt
     2761 cttcgtggag atggagaagg gggcccgcaa ggcggaacag gagttgaaca tcaatctcat
     2821 cgtgaagacg ggagcgaagg aaacatctat agagcagcag atcgccatcg tcgatgaact
     2881 gatccgcaac cgggtggaag ccatcgtcat cgccccggcc agctccgtgg agatcatccc
     2941 catcctcaag aaggcccagg atacgaagat cgtcatcgtc aacatcgaca atcaggtcga
     3001 tcccgaactc tcccggaaga tgggcttgac ggaggtgcca ttcatcagcg tggataacga
     3061 aaaaggggcc tatctggccg ccggtacatc agcgacggga tcaagaaacc gaccaaggtc
     3121 gccattctcg aaggaatcag gagcgcgaag agcgccgaac agcgccgaac agcgcaaggc
     3181 cgacgccgta aaggccttcg gggagaatcg gaacatccgc atcgtcgcct ccgaaacggc
     3241 caattggagg atcgacgagg ccttcgacgt gacggcgaaa atctttaatc gtgtcgcttc
     3301 ctgccgttgt ttatcgcccc cgaaacccgc aagattctga ttattaccgc tgcgtcgagg
     3361 accacttcga gacctttgtc cggatctatg acgatcgttt cttccggcag tacggattct
     3421 ggcggcctta tctggaaagg gtcatatacc gcggagaccc ccaatactcc tgggacgatt
     3481 acatccaggc atgacgcttt gaaaaat
//
LOCUS       DAOSMF010000187         3486 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_29294_length_3486_cov_24.833285, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000187 DAOSMF010000000
VERSION     DAOSMF010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3486
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29294_length_3486_cov_24.833285"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            529..885
                     /locus_tag="PLT63_10775"
     CDS             529..885
                     /locus_tag="PLT63_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HOD99246.1"
                     /translation="MSARLEREIDTIGLMIEMFCRDHHGRDGLCSFCRELLAYARKRT
                     RACRFGKDKPVCSACPVHCYRPEMRERIREVMRYSGPRMISRHPVLAVRHLLDKRHKT
                     PPRLAKRETAGQTKQS"
     gene            894..2726
                     /gene="polX"
                     /locus_tag="PLT63_10780"
     CDS             894..2726
                     /gene="polX"
                     /locus_tag="PLT63_10780"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HOD99247.1"
                     /translation="MKLSKQEIIAALDELALLLELKEENPFKSRAYANAARSLEARED
                     DLEAIIAAGRLGEIKGVGTAIARKIEELAATGELRYHRELKASIPSGHFEMLRIPSLG
                     PKRIRFLYEKLGIATVGELEYAAQENRLLDLPGFGRKLQEKILAGIAALKHYRERRLY
                     GEAIGPALRLQATLLSRPEVSAADVAGSLRRGNETVKDIDLLAATADPPRLAAYFASL
                     PEAAAVIARGDTKVSITLREGINADLRIVSPLQYPYALHHFTGSREHNTALRGRAKRY
                     GLKMNEYGLFRGEENIPCRSEEEIFAALELAFIPPELRENMGEIEAAARNALPHLVEP
                     NDIKGILHIHSRASDGADGIEALAAEARRRGYEYIGISDHSQAAAYAGGLIPEDVREQ
                     HEEIDRINGRYGGVFHIFKGIEADILPDGRLDYDEEILASFDFVIAAVHSHFQMSEED
                     MTRRICRAMANPFTTILAHPTGRLLLAREPYALRIDAVIDAAAAHGKVIEINANPLRL
                     DLDWRHCIQAKRKGVKVAVNPDLHRLDGFDYLEYGIAVARKGWQEKGDCLNCLDTEGI
                     KSFFQDLKNRARGDATGGKEDRAGWRPEQQHLGESVPMLNLRDR"
     gene            2723..>3486
                     /locus_tag="PLT63_10785"
     CDS             2723..>3486
                     /locus_tag="PLT63_10785"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF016169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HOD99248.1"
                     /translation="MSLRIVFSNRMEILGQLLMEELGRIPADPFAPQHVIVPNAAVTR
                     YLQLAIARKLDICANVKFSFLGHWLWQLARSVDPSVPERSPVDPEIMSWLVLRLLEEH
                     RFTAFPRLAGFLAGADDLMRLDLARAVARVFDHYATYRPDWLAAWNAGGGIPEFRGRP
                     LMEADEDWQRELWLAIGRELSLKGVHPLLSLFGYRSSLAPDRPEGTSSLPQSAAVFAL
                     PVIPPLYLHALRRLAAIMDITLYLLNPCRQYWFEIVP"
BASE COUNT          701 a         1097 c         1031 g          657 t
ORIGIN      
        1 gaaatgaata ggtaaattta ccgaggtggt tctgacgcct cttggcctgc ctgtcctgct
       61 ggaggtatat cgcatcacgg ccgggtcatc tccttaaact tcagggattt caagcgcaaa
      121 gacgctcttt tgttgaggtg atggcttgaa atgtatggat cggtgaatgg ctgtgctcgc
      181 cttaaccgca catccacggc gcttatatac ctttcatgtt cggttgtcaa gactaatccc
      241 gatgtttttc tggtggttta ttcattcttg cccgatccgg cggggatcat ttcttgacat
      301 ccgggaaaca agaatttact gttgcggtga cgggcaccga accgacggtg gcgggaagcc
      361 tacgggcggc accgtggata aaacatcaac aatgtaggtg gtcaagcgcc aaggtttcgc
      421 ttatcccggt ttcaatcggg ccgagagtat ttttctccgg cacgggatag agtcgttccc
      481 ggccgggaca aacagcgatg cttaacaagg catcaggatg gttgagaaat gagcgcccgg
      541 ctggaaaggg agatagatac catcggtttg atgatcgaga tgttctgccg cgatcatcac
      601 ggtcgggacg ggctctgttc cttctgccgc gagctgctgg cctatgcccg gaaacggacc
      661 cgggcctgcc gcttcggcaa ggataaaccc gtctgctccg cttgccccgt ccactgctac
      721 cgtccggaga tgcgggagcg catccgggag gtgatgcgct attccggacc gcggatgatc
      781 tcccgccatc ccgttctggc ggtgcggcac cttctggaca aacgacataa aacgccgccg
      841 cggctcgcga aaagggaaac ggccggccaa accaagcagt catgaccgtc ggcatgaaac
      901 tatcgaaaca agaaatcatt gccgccctcg acgagctggc cctcttgctg gaactaaagg
      961 aggaaaaccc tttcaagtcc agggcctacg ccaatgccgc ccggagcctg gaggcccggg
     1021 aagacgatct ggaagcgatc atcgcggcgg ggaggcttgg cgaaatcaaa ggggtgggca
     1081 ccgccatcgc caggaagatc gaggagttgg cggcgacggg ggagttgcgc taccaccggg
     1141 agctaaaggc atccatcccc tccggccatt tcgagatgct ccgaatcccc agcctcggtc
     1201 cgaaacggat caggttcctc tacgagaagc tgggcatcgc caccgtcgga gaattggaat
     1261 acgccgccca ggaaaaccgt ctcctggacc tgccgggctt cgggcggaaa ttacaggaaa
     1321 agatcctggc gggcatcgcc gccctgaagc attaccggga acggcgcctg tacggagagg
     1381 cgatcggccc ggcgctccgg ctccaagcga cccttctcag ccggccggag gtctctgccg
     1441 ccgacgtagc cggatcgctg cggaggggaa atgaaaccgt aaaagacatc gatctgctgg
     1501 ccgccaccgc tgatccgccg cggctggccg cctacttcgc ttccctgccc gaggcggccg
     1561 ccgtcatcgc ccggggggac accaaggtca gcatcaccct gcgggagggg atcaacgccg
     1621 atctccgcat cgtttccccc ctgcaatacc cttacgccct ccatcacttc acgggcagcc
     1681 gggagcataa taccgccctg cgggggcggg ctaagcgcta tggtctgaag atgaacgaat
     1741 acggcctttt ccggggagag gaaaacatac cctgccgaag cgaggaggag atcttcgccg
     1801 ccctggaact ggccttcatc cccccggaat tgcgggagaa catgggagag atcgaagcgg
     1861 ccgcccggaa cgccctgccc caccttgtag aaccgaacga tataaaaggg atattgcaca
     1921 tccacagccg ggccagtgat ggcgcggacg gtatcgaggc cctggccgcg gaggcccggc
     1981 ggcggggtta cgagtatatt ggcatcagcg accacagcca ggcggccgcc tatgccggcg
     2041 gcctgatccc ggaggacgtg cgggagcagc atgaggaaat cgaccgcatc aatgggcgct
     2101 acggcggcgt cttccatatc ttcaagggca tagaggcgga tatcctcccg gacggccgcc
     2161 tggattacga cgaggaaatc ctggcttcct tcgatttcgt catcgccgcc gtccattccc
     2221 acttccagat gtccgaggaa gacatgacca ggaggatctg ccgggccatg gccaatccct
     2281 tcaccactat cctcgcccac cccacgggcc gtctcctgct ggcccgggaa ccctatgccc
     2341 tcaggatcga cgcggtcatc gacgccgctg ccgcgcatgg caaggtcatt gaaatcaatg
     2401 caaaccccct gcgccttgac ctcgactggc ggcactgcat tcaggccaag cgcaaagggg
     2461 tgaaggtcgc cgtcaatccc gacctccacc gcctcgacgg cttcgactac ctggagtacg
     2521 gcatcgccgt cgcccggaag gggtggcagg aaaagggcga ctgcctgaac tgcctcgata
     2581 cggaaggaat aaagagtttc tttcaggacc tgaaaaaccg cgcacggggg gacgcgaccg
     2641 gcggcaaaga agatcgcgct ggttggcgtc ctgaacaaca gcaccttggg gagagcgtcc
     2701 ccatgttgaa tcttcgggac cgatgagcct gcgcatcgtc ttttcaaatc gaatggaaat
     2761 cctgggacaa ctgctcatgg aggaactggg ccgcataccc gcggacccct tcgcgcccca
     2821 gcacgtcatc gtacccaatg ccgccgtcac ccgttacctc caactcgcca ttgcccgcaa
     2881 gttggacatc tgcgctaatg taaaattcag ttttctcggc cactggctct ggcaactggc
     2941 ccggtccgtc gatccctccg tgccggagcg ttcccccgtc gatcccgaga tcatgtcctg
     3001 gctggtgctg cgtcttctgg aggaacaccg gtttaccgct tttccccgtc tggccggatt
     3061 tctcgccggc gccgacgatt tgatgcgtct agacctcgcc cgggccgtgg cacgggtctt
     3121 cgaccattac gccacctatc gccccgattg gctggcggcc tggaatgccg ggggtgggat
     3181 ccccgaattc cggggccggc ccctcatgga agccgacgag gattggcaac gagaactatg
     3241 gctggccatc ggccgggagc tatccctgaa gggcgttcat cccctgcttt ccttattcgg
     3301 atatcggtcg tcgctggcgc cggaccgccc cgaagggacg tcatcgctac cgcaaagcgc
     3361 cgccgtgttc gcccttccgg ttattccgcc cctctacctg cacgccctgc gccgactcgc
     3421 cgccatcatg gacatcaccc tgtatctgct caacccctgc cgccaatact ggttcgagat
     3481 cgtgcc
//
LOCUS       DAOSMF010000188         3485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_29306_length_3485_cov_22.848688, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000188 DAOSMF010000000
VERSION     DAOSMF010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29306_length_3485_cov_22.848688"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..853)
                     /gene="thrC"
                     /locus_tag="PLT63_10790"
     CDS             complement(<1..853)
                     /gene="thrC"
                     /locus_tag="PLT63_10790"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOD99249.1"
                     /translation="MSMPIRYYSTNRNLGLSGGGKPFRETVSFREALLMGQAPDEGLF
                     MPERIPALKLADVAALKGRPYPEAAWLVASKFLAGEMDLDRLHWVVEDSYNFSVPLEH
                     VAGECFVLRLDQGPTASFKDFAARMMARLMSMLRPADKRLNVLVATSGDTGSAVGEAF
                     KGVPGIDVTILYPRDEVSSRQKKQLDTIGQNVQAVSVAGKFDDCQDMVKQAFADPSLA
                     ELNLTSANSINFGRILPQTIYYVYAYAQLARVEAGEEIVFSVPSGNFGNALGCEYARR
                     MGLPVRML"
     gene            1149..2204
                     /gene="selD"
                     /locus_tag="PLT63_10795"
     CDS             1149..2204
                     /gene="selD"
                     /locus_tag="PLT63_10795"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013842813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:1200..1202,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HOD99250.1"
                     /translation="MTKENRPRLTQTVHGAGUACKIGPGDLHKALKDLPFMTDPHLLA
                     GMEHSEDAGVYKLRDDLAIIQTVDFFTPIVDDPYTFGQIAVTNALSDVYAMRGNPLTA
                     MNIVCFPINKLDITILRDILRGGLDKMREAGVLLVGGHSVEDDETKYGLAVTGIAHPD
                     QILLNRGARPGDRLILTKALGTGIVSTAVKGGIATESLVNEAVRAMTTLNKSGAQALM
                     ETPEVHSCTDITGFGLLGHACEMIEDSSVGMEIHAGAVPFFSGLREIAELGVLPGGLH
                     RNRRFRENRIEIDPGCPEWQVDILFDPQTAGGLFVAVAPAEAERLLDKLHRAGLGEAA
                     SIGRVTDERKGKILVRP"
     gene            2201..3274
                     /locus_tag="PLT63_10800"
     CDS             2201..3274
                     /locus_tag="PLT63_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99251.1"
                     /translation="MKRIESLLVKKEETLFNVFKLRSMARPEDTMAVLMFGSPDPDAV
                     ASAMALREIMRQTVGLKKFAFVATEGVLRQQNQEFIRAMKVEIQLLSKIELQDYRLVA
                     IVDAQPNFFGKTGDLIHPQIVIDHHPVTAAWHAELADVRPQYGALSTIMMEYLLAARV
                     RIPKLLYTALLYGIKSDTNNFERDSILEDIGAYYLTSVRANRELIRRIELNQIPERYL
                     KSFEHAYFHRRRYRDRITSFLGKVDSPDVCVQVADFYLRIVDVYYVVIAGIIKDRLII
                     VFRGDGYRQDCGYIAAAAFGKYGGGGGHKSAARVEIPLETLKEVLPGDWTQEAVEQFL
                     VQRLRQKRPAKTKKRPANDARES"
BASE COUNT          764 a         1098 c          971 g          652 t
ORIGIN      
        1 ccagcatccg caccggcaga cccatgcggc gggcatattc acagcccagg gcgtttccga
       61 aattgccgga aggaacggaa aaaacaattt cctctcccgc ctccacccgt gccaactggg
      121 cgtaggcata gacgtaatag atcgtctgcg gcagaatacg gccgaaattt atcgaattgg
      181 ccgacgtcag attgagttcc gccagggagg gatcggcgaa ggcctgtttc accatgtcct
      241 ggcaatcatc gaatttcccg gccacggaga ccgcctggac gttttgcccg atggtatcta
      301 gctgcttctt ctggcgggag ctgacttcat cgcggggata gaggatcgtc acatctatcc
      361 ccggcacccc tttgaaggcc tcccccacgg cgctccccgt atctcccgag gtcgccacca
      421 ggacattgag gcgtttgtcc gccggccgga gcatgctcat gagacgggcc atcatccggg
      481 ccgcgaagtc cttgaaagaa gccgtcggcc cctgatcgag gcgcaggaca aagcactctc
      541 ccgccacatg ttccagaggc acggaaaagt tgtaggagtc ttccaccacc cagtgcaatc
      601 tatcaagatc catctctccg gcgagaaatt tactggccac cagccacgcg gcttccgggt
      661 aaggcctgcc cttcaaggcg gcgacatccg ccagcttaag ggctggaatc ctctcgggca
      721 tgaacagacc ctcatccggt gcctgtccca tgagaagcgc ctccctgaaa ctgaccgttt
      781 ctcggaatgg cttcccgccg ccactcaggc ccagatttct attggtgctg taataacgaa
      841 tcggcattga cattttctgc tcctgtcccc aaggttctcc ggcccggtaa ccgagagatc
      901 gcctcctccg gcggatgggc aaaaaaaccg gagctattca aaactccgga aaatgtttac
      961 cttcccgatc tttccctgtc aagcatcttc tcttcagatt tttctcggca aaacgttccc
     1021 tcaaaaccga gaagtgccaa cctgtatcga taagggtgtc atcttcccga ttgacagaaa
     1081 gggtttttcg gcggataatc agccccaaag attaatgttc gtccataact gccgctgagg
     1141 attcaacaat gacgaaagaa aaccgcccgc gccttaccca gaccgtccac ggcgccggct
     1201 gagcttgcaa gataggtccg ggcgaccttc acaaagcgct gaaagatcta cccttcatga
     1261 cggatcccca tctcctggcc gggatggaac actccgaaga cgccggggtc tataaactcc
     1321 gcgacgatct ggcgattatc cagacggtgg atttcttcac ccccatagtc gatgaccctt
     1381 acaccttcgg ccagatcgcc gtgaccaacg ccctcagcga tgtctatgcc atgcggggca
     1441 atcccctgac agcgatgaac atcgtctgct ttcccatcaa taaactcgat attaccatcc
     1501 tgcgggacat tctccggggc gggctggaca agatgcggga ggcgggcgtc ctcctggtcg
     1561 gcgggcacag cgtcgaggac gacgagacca aatacggcct cgccgtgacg ggaatcgccc
     1621 atcccgatca gatcctcttg aaccggggcg cccggccggg agacaggctc atcctgacca
     1681 aggccctcgg cacgggaata gtcagcaccg ccgtcaaggg ggggatagct acagagagcc
     1741 tggtcaacga ggccgtccgc gccatgacga ccctgaacaa gtccggggcg caagccctaa
     1801 tggagacacc ggaggtccat tcctgcaccg acatcaccgg gttcggtctc ctggggcacg
     1861 cctgcgagat gatcgaggac agctcggtgg gcatggagat ccatgccggc gccgtgcctt
     1921 tcttcagcgg cctgcgggaa attgccgagc tgggcgtgct gcccggcggg ctccaccgca
     1981 accgacggtt ccgggaaaac cggatcgaaa tcgatccggg atgcccagaa tggcaggtgg
     2041 acatcctctt cgacccccaa acggccgggg gacttttcgt cgccgtagcc ccggcggagg
     2101 cggagcggct actggataag ctccaccgcg cgggccttgg cgaggccgcg agtatcggtc
     2161 gagtcaccga cgaaaggaaa ggaaagattc tcgtccggcc atgaaacgca ttgaatcgct
     2221 cctggttaaa aaggaggaaa ccctcttcaa cgtcttcaag ctgcgttcca tggcccggcc
     2281 ggaggatacc atggcggtgc tcatgttcgg cagccccgat ccggatgccg tcgcctcggc
     2341 gatggccctg cgggaaatca tgcgacagac ggtggggctg aaaaaattcg ccttcgtcgc
     2401 cacggaaggg gtgctccgcc agcagaacca ggaattcatc cgggccatga aggtggaaat
     2461 tcagctcctg agtaagatag agctccagga ctaccgactc gtcgccatcg tggacgccca
     2521 gcccaacttc ttcgggaaga ccggcgatct gatccacccc caaatcgtca tcgaccacca
     2581 tcccgttacc gccgcctggc atgccgaact ggcggatgtc cgcccccagt acggcgccct
     2641 ctccaccatc atgatggagt atctcctcgc agcccgggtc cggataccca agctgctcta
     2701 caccgccctg ctgtatggca tcaagagcga caccaacaat ttcgagcggg attcaatcct
     2761 cgaggacatc ggggcttatt acctgacctc ggttcgggcc aaccgggagc tcatccgtcg
     2821 catcgaactg aaccagattc ccgagcgcta cctgaagtct ttcgaacatg cctatttcca
     2881 ccgtcgccgc taccgcgacc ggatcaccag tttcctgggc aaggtggaca gccccgatgt
     2941 atgcgtccag gtagccgatt tctatctgcg catcgtcgat gtttactacg tggttatcgc
     3001 cgggatcatc aaagaccggc tgatcatcgt ctttcggggc gacggttacc gtcaggactg
     3061 cggctacatc gcggcggcgg ccttcgggaa atacggcggc ggcggcggac acaagagcgc
     3121 ggcccgggtg gaaatccccc tggaaacact caaagaggtc ctcccagggg attggaccca
     3181 ggaagcggtg gaacagtttc tcgtccagcg cctccgtcag aaacgcccgg caaagacgaa
     3241 gaaacgcccg gcaaacgacg ccagggaatc ctgactccct ttctccagct tatatccata
     3301 cattcccacc gaacggtaga gccattcccg ccgggagcaa taaagtcagc tcaggtggga
     3361 tgcgacgggc agcggcatct tgatcttttg ggtgaagtag agcgaaacca ccgtaagacc
     3421 ggctccgatt agaaagggca gccgccagtt caatagccac aggctgccgc cgatcagggg
     3481 gatga
//
LOCUS       DAOSMF010000189         3456 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_29615_length_3456_cov_25.598647, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000189 DAOSMF010000000
VERSION     DAOSMF010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29615_length_3456_cov_25.598647"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..3111)
                     /gene="fdnG"
                     /locus_tag="PLT63_10805"
     CDS             complement(10..3111)
                     /gene="fdnG"
                     /locus_tag="PLT63_10805"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(2554..2556),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HOD99252.1"
                     /translation="MDVSRRGFLKISGAVLAASGLGVSLKPVSAYAQPLKIKYAKETT
                     TICPYCSVGCSIIVSVRQGKVVNTEGDPDSPINKGSLCSKGGSIYQMAVNENRLGKPL
                     YREPYGTEWKEVDWEWAFEKIAENIKNSRDASFKVANEKGELVNRTEGIASVGSAAMD
                     LEECFTYQKFLRSLGLVYIEHQARIUHSPTVPALAESFGRGAMTNHWVDFRNADVILI
                     MGSNPASNHPVSFKWIQEAVDKRGARILCVDPRFTQSAAKAHVYAPIRSGTDIAFLGG
                     MIKYIIDNNLFFDEYVKNYTNASFLVNPAFKLPGDNNGVFSGLEGAKYNKETWSYQTG
                     KDGVILKDPTLQDPNCVFQLLKKHYSRYTPEMVTKITGTPTDKLLEIYKEYASTGRPD
                     RAGVELYAMGWTQHTVGTQNIRTMCMIQLLLGNMGIAGGGVAAMRGESNVQGSTDHGL
                     LFHIWPGYLGTPTGSIKTLKDYNEKRTPKTNEKNSLNWWKNFPKYSASFLRSMYGTNA
                     SLEEAYAMMPKVDDGANYSWLTIFDQMYKGKFTGFFAWGMNPACSGAHSNKVRQALAK
                     VDWLVNVNLFDNETGSFWRGPGMDPTKIKTEVFMLPCAASIEKEGSLANSSRLQQWRY
                     KAVNPPGVALPDGDIMSEVFFKVKELYAKQTGAFKDPIMKLTWPYGKKTVAGHFHYDP
                     RMVAAEINGFFLEDKKVEIPNKKGEFKEFKKGDLVPTFAWLQDDGSTSSGCWVYCGSV
                     SDKGILPMRRGLKDPTGIGIYPEWSWSWPVNRRIIYNGASVDPNGKPWDPKRAVITWD
                     GSKWVGDVPDGAGDPGSGRPPFIMKPDGVASLFGPGLADGPFPEHYEPLECPVEKNLL
                     SPQKNNPAVKRFDKPGVGSDLDVYSGVASCDPRFPFICSTYRVSEHWQTGVLTRWCPW
                     LAEMQPGMFVEMSVELAREKGIVNGDKVIVKSARGQVECTAIVTPRFKPFNIDGNTIH
                     EVGIPWHFGWITTKDRKYQPGDKKAEVFTFGDAANLLTPTIGDANTMIPESKAFMVNV
                     IKK"
     regulatory      complement(3147..3266)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
BASE COUNT          678 a          995 c          979 g          804 t
ORIGIN      
        1 ctcaccccct cacttcttga tcacattgac catgaaggcc ttgctctcag ggatcatggt
       61 attggcatcg ccaatggtcg gcgtcagcag gttcgctgca tcgccgaagg taaagacctc
      121 ggccttcttg tcgcccggct ggtacttccg gtccttcgtc gtgatccacc cgaaatgcca
      181 ggggatgccg acctcgtgaa tcgtattccc gtcgatattg aaaggcttga aacggggcgt
      241 aacgatggcc gtgcactcca cctgaccgcg cgccgatttc acgatcacct tgtcgccgtt
      301 cacgatgccc ttctcccggg ccaactccac gctcatctcc acaaacatcc ccggctgcat
      361 ctccgccagc cacgggcacc accgcgtcag aacgccggtc tgccagtgct cgctcacccg
      421 gtaggtggaa cagatgaagg ggaagcgggg atcgcaactc gccaccccgg aatagacatc
      481 cagatcggag cccactccgg gcttgtcgaa acgcttgacc gccgggttgt ttttctgggg
      541 tgacaggaga ttcttctcca cggggcactc caggggctcg taatgctcgg ggaagggacc
      601 atcggcaaga ccggggccga agaggctggc cacgccgtcg ggtttcatga tgaacggcgg
      661 ccttcccgaa ccgggatctc cggcgccgtc ggggacgtcg ccgacccact tgctgccgtc
      721 ccaggtaatg accgctcgct tgggatccca gggcttgccg ttgggatcca cggaggcgcc
      781 gttatagatg atccggcggt tcaccggcca ggaccaggac cactcaggat aaataccgat
      841 ccccgtggga tccttcagcc cccggcgcat gggcaggatg cccttgtcgg agaccgaacc
      901 gcagtaaacc cagcaacccg atgatgtgga accgtcatcc tggagccagg caaaggtagg
      961 taccagatcg cccttcttga actccttgaa ttcccctttc ttgttgggga tttccacctt
     1021 cttgtcttcc aggaagaaac cgttgatttc agcggccacc atccggggat cgtagtggaa
     1081 atgaccggca acggtctttt tgccataagg ccaggtgagt ttcatgatgg ggtctttgaa
     1141 ggcgccggtc tgcttggcgt ataattcttt gaccttgaag aagacctcgc tcatgatgtc
     1201 gccgtcggga agagccaccc cgggcggatt gacggccttg tagcgccact gctgcagccg
     1261 gctgctgttg gccagacttc cttccttctc gatggaggcg gcgcagggca gcatgaatac
     1321 ctccgtcttg atcttggtgg gatccatgcc gggaccgcgc cagaaggatc cggtctcgtt
     1381 gtcgaagaga ttcacgttga ccagccaatc gaccttggcc agggcctggc ggaccttgtt
     1441 ggaatgggcg ccgctgcacg ccgggttcat cccccaggcg aaaaagccgg tgaatttacc
     1501 cttgtacatc tggtcgaaga tcgtcagcca ggaatagtta gcgccgtcgt ccaccttggg
     1561 catcatggca taggcctctt ccagggaggc gttcgttcca tacatagacc ggaggaaact
     1621 cgccgagtac ttggggaaat tcttccacca gttgaggctg ttcttttcgt tcgtctttgg
     1681 tgtccgcttc tcgttgtaat ctttcagggt cttgatcgat cccgtggggg tccccagata
     1741 gcccggccag atgtggaaga gcaggccgtg gtccgtggag ccctggacgt tggattcgcc
     1801 ccgcatggcc gccacgccgc cgccggcgat gcccatgttg cccaggagca actggatcat
     1861 gcacatcgtc cggatgttct gggtgcccac ggtatgctgg gtccagccca tggcgtaaag
     1921 ttccaccccc gcccggtccg gacggcccgt ggaggcgtat tccttataaa tctcgaggag
     1981 cttgtccgta ggcgttccgg tgatcttcgt taccatttcc ggggtatagc gggaataatg
     2041 tttcttcagg agctggaaga cgcagttcgg gtcctggagg gtgggatcct tgaggatcac
     2101 gccgtccttg cccgtctgat aggaccaggt ctccttgttg tacttggccc cttccagacc
     2161 cgagaagaca ccgttgttgt ccccggggag cttgaaggcg ggattcacca ggaaggaggc
     2221 attggtatag ttcttcacat attcgtcaaa gaagaggttg ttgtcgatga tgtacttgat
     2281 catgccgccg aggaaggcaa tgtccgtccc cgagcggatg ggggcgtaaa catgggcctt
     2341 ggccgccgac tgggtgaacc gcgggtccac gcagaggatt cgcgcccctc ttttgtcaac
     2401 ggcttcctgg atccacttga aggaaaccgg gtgattggaa gcggggttgc ttcccatgat
     2461 caggatcaca tccgcattgc ggaagtcgac ccagtgattc gtcattgcgc cgcgtccgaa
     2521 cgactctgcc agagccggta cagttgggct gtgtcagatc cgggcctggt gttcaatata
     2581 gaccagaccc aagctcctta agaatttttg gtaggtaaag cattcttcga ggtccatggc
     2641 ggcgctgcct acggaagcaa tcccctctgt ccgattgacg agctcgccct tttcgttggc
     2701 gaccttgaag ctggcatctc tgctgttctt gatattctcc gcaatcttct cgaaagccca
     2761 ttcccagtca acttccttcc attccgtgcc atagggttct cgataaaggg gtttgcccag
     2821 acggttctcg ttgacggcca tctgatagat ggaaccaccc ttactgcaca gcgagccctt
     2881 gttgatggga ctgtccggat cgccttccgt gttgacaacc ttaccttgtc gtacggaaac
     2941 gatgatgctg caaccgacgg aacagtatgg gcagatcgtc gtggtctctt tggcatactt
     3001 gatcttcagg ggttgagcat aggcggagac gggcttcagg cttacgccca agccgctggc
     3061 cgccagcacc gcaccggaga tcttcaagaa acctcttctg ctgacatcca taaaatgcgc
     3121 tcctttctca gatatttact ttcaatatcc cgtccatcga ctcgtctttt ccaatcatgg
     3181 gaggcgccat cgtttccagc ggcacccggc atccgttttt ccttgaaggt cgctctagaa
     3241 aaaacggctt ggcgagatga tgcactcggt tttccagaag gcctgaaata accggttccg
     3301 tatatatgct acgccgtttg gtgtcaagat ttttctgacg ataaaaccaa tgatttcaag
     3361 gtcttatgtc atttatgaca tatgggattt agcggagaga taacagtcta aaaccagagg
     3421 gttccatccc cggtggcatc cgggcagata gaaaat
//
LOCUS       DAOSMF010000190         3440 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_29795_length_3440_cov_23.139439, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000190 DAOSMF010000000
VERSION     DAOSMF010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29795_length_3440_cov_23.139439"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="PLT63_10810"
     CDS             complement(<1..721)
                     /locus_tag="PLT63_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99253.1"
                     /translation="MRNLLKNIAALAFVLLLGLYLAEWLLGFYLVRAVPRYPLPPFAV
                     QRHATVDYDVAYRYNNHGLRGPDFHPGEAYDAVLFGDSFFFGQGVAEGKTITDNMRGK
                     GLKVLNVSEIATNPSDYFHKLNVMKSQGLRSRTVVIGLCMGNDFQDIADKDIGPALAH
                     QYRTPFLAYDVRSFLTLARLRYQLGRKTRQIKDWFNYRFFGAPDRETLMVHEFEHRRK
                     FHSDWLRFFADNRPEIMAAMTG"
     gene            881..2278
                     /locus_tag="PLT63_10815"
     CDS             881..2278
                     /locus_tag="PLT63_10815"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOD99254.1"
                     /translation="MARLYSYPGTGSCMKGKYAATAPKNIGHIVVIAAVLMMAVALVI
                     NEWTLAWWSCPDGHFEPDDRLRIAAFQGLCLLGGVLLLVFRRKALTVNILLSAATILV
                     CFSLGEILMRNFHPQIGGRSEQEIFFAYDETLGWRLIPGKSGLLVSKHEYENIININA
                     AGFRDRERPLAKASGTRRIAVIGDSFTAGLGVKDPEVFTAVLEATLPGATEVLNFGVN
                     GFGPTQEYLLLTTRVFPYHPDLVVMVFYIGNDFDDTTNLTAWIDGYQRPRAVLDHRGR
                     ISFTNIPVPKTSRPRNERRQTCSLPRSHLVDFFDRILHQRRDKYALDVLPPEVRLCRK
                     DLDPSLQEALPLLRGILRETDARCRRQGGRFALVLAPTVTQIHADIYWRKIKKVYNLR
                     EEDYDLLLPNKILTGIAGDLGIPVLDLTPALRSEADRGAELYYYKNRHWNAAGHRAVA
                     RELAAWIETKKLLTE"
     gene            2245..>3440
                     /locus_tag="PLT63_10820"
     CDS             2245..>3440
                     /locus_tag="PLT63_10820"
                     /inference="COORDINATES: protein motif:HMM:NF014589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HOD99255.1"
                     /translation="MDRDKEAVDRITYYAYKAGHLSKDNIRNVMHILGISAFYHDSAA
                     ALLKDGEIVAAAQEERFTRKKHDPSFPAHAASFCLQRARIGVEDLDAVVFYDKPLLKF
                     ERLLETYYAFAPRGLKSFLSAIPVWIKEKVFLKKLIRDELKRIGNGTAKGVKLLFTDH
                     HLAHAASAFFPSPFPEAAILTIDGVGEWATASIGHGRDNDITLLKEMRFPHSVGLLYS
                     AFTHYLGFRVNSGEYKLMGLAPYGDPDSEQVRRYREAIRTHLVDVKEDGSIWLNQDYF
                     DYATGLSMVKDDKWAAIFGFPRRTPETAIEQRHGDLAYAIQQVTEEIVMKMARTARRL
                     TGAENLCLSGGVALNCVANGKLLRSGDFAGIWIQSAAGDAGGALGAAYAIHHMYHQKP
                     RPRADGG"
BASE COUNT          688 a         1103 c         1007 g          642 t
ORIGIN      
        1 aacccgtcat ggccgccatg atctccgggc ggttgtccgc aaagaagcgc agccagtcgg
       61 aatggaattt ccgccggtgc tcgaattcat gcaccatcag tgtctcgcga tcgggagcgc
      121 cgaaaaaccg gtaattgaac cagtccttga tttgccgggt ttttctgccc agttgataac
      181 ggaggcgcgc cagggtcagg aaggaccgga cgtcgtaggc cagaaaaggg gtgcgatatt
      241 gatgggccag cgccggcccg atgtccttgt ccgcgatgtc ctgaaaatcg ttgcccatgc
      301 acaggccgat gaccacggtc cggctccgga gcccctgtga cttcatgacg ttgagcttgt
      361 ggaaataatc gctggggttg gtggcgatct cggagacgtt caggaccttg aggccctttc
      421 cccgcatatt atcggtgatg gttttcccct ccgcgacgcc ctggccgaaa aagaaagaat
      481 cgccgaaaag aacggcgtca tatgcctcgc cgggatgaaa atccggtccc cggaggccat
      541 ggttgttata acgataggcg acgtcgtaat ccaccgtcgc atgcctttgc acggcaaagg
      601 gaggcagggg ataacgggga accgcccgga ccagataaaa gcccaggagc cactcggcaa
      661 gatacaggcc caagagcagg acaaaggcaa gtgctgcgat gttcttgagc aggttacgca
      721 tggggcgact aatagcatgt ccggaggatg gcgtcaaaaa ttatgcagtt ggaccggttt
      781 cgccggcggc aatacacata aatcgtggca ttatcggccc cgccgtgata aaaggcccca
      841 tgtcccgggg agggccacga agcaggaaga cgacgttttc atggccaggc tctattcgta
      901 tccgggtacg ggatcgtgca tgaaggggaa atacgccgcc acggcgccga aaaatatcgg
      961 gcacattgtc gtcatcgccg cggtcctgat gatggccgtc gccctggtga tcaatgaatg
     1021 gaccctggcc tggtggagct gtccggacgg acatttcgaa ccggacgacc gcctgcggat
     1081 cgccgccttt caggggctgt gccttctcgg cggcgtgttg ctgctcgtct tccggaggaa
     1141 ggcgctcacc gtcaatatcc tcctttctgc cgctacgatc ctggtctgtt tctccttggg
     1201 agagatcctg atgcggaatt tccatcccca gatcgggggg aggtcggaac aggagatatt
     1261 cttcgcctat gacgagaccc tgggctggcg gctcatcccg ggaaaatcgg gcctcctcgt
     1321 ttccaaacat gaatatgaga acataatcaa catcaacgcc gcgggttttc gggatcggga
     1381 acgccccctt gccaaggcct cgggaacaag aaggatcgcc gtgatcggcg attccttcac
     1441 ggccggcctg ggcgtcaagg acccagaggt gttcaccgcc gtcctggagg cgaccctacc
     1501 gggcgctacg gaggtcctca atttcggcgt caacggcttc ggtcccaccc aggagtatct
     1561 cctcctcacc accagggtct ttccctacca ccccgatctc gtcgtgatgg tcttctatat
     1621 cggcaacgat ttcgacgaca ccacgaatct caccgcctgg atcgacggct accagcgtcc
     1681 ccgggccgtt ctggatcacc ggggccggat ctccttcacg aacatccccg tcccgaaaac
     1741 ctcccgtccc cggaacgagc gccggcagac atgcagcctt ccccgctccc acctcgtcga
     1801 tttcttcgac cggattctcc accagcgacg ggacaaatac gccctggacg tcctgccgcc
     1861 ggaggtgcgg ctttgccgca aagacctgga tccatcactg caggaggccc tgcccctgct
     1921 ccggggcatc ctccgggaaa cggacgcccg ctgccggcgt cagggcggcc ggtttgccct
     1981 ggtcctcgcc cccaccgtca cccagatcca cgcagatatt tactggcgaa agatcaagaa
     2041 ggtctataac ctccgggaag aggattacga tctcctgctc cccaataaaa tcctcaccgg
     2101 catcgccggc gacctcggca tccccgttct ggatctgacc ccggccctgc ggtccgaggc
     2161 cgaccgggga gccgaattgt actactacaa gaatcgccac tggaacgccg ccggacaccg
     2221 cgccgtcgcc cgggagctgg cggcatggat cgagacaaag aagctgttga cagaataacc
     2281 tattatgcct ataaggctgg acatttatcg aaggacaaca tcaggaacgt catgcatatc
     2341 ttaggcatct ccgcttttta tcacgattcc gccgccgccc tcctcaagga cggcgagatc
     2401 gtcgccgccg cccaggagga gcgcttcacc cggaagaagc acgacccatc cttcccggcc
     2461 catgcggcgt ccttctgcct gcagagggcc cggatcggcg tggaggatct ggacgccgtg
     2521 gtcttctacg acaagccgct gctgaaattc gagcggcttt tggagaccta ctacgccttc
     2581 gccccccggg gtctgaagtc ctttctctcc gccattcccg tgtggatcaa ggagaaggtg
     2641 ttcctcaaga aactgatccg cgacgagctg aagcggatcg gcaacggcac cgcgaaaggt
     2701 gtcaagctcc tcttcacgga tcaccacctg gcccacgccg ccagcgcctt cttcccctct
     2761 ccctttcccg aagcggcgat cctgaccatc gacggcgtcg gggaatgggc caccgcctcc
     2821 atcggccacg gccgggacaa cgacatcacc ctcctcaagg agatgcgctt cccccattcc
     2881 gtcgggctgc tctactccgc cttcacccac tatctgggct tccgggtcaa ttccggggag
     2941 tacaagctca tggggctggc cccttacggc gatcccgatt ccgaacaggt ccggcgctac
     3001 cgggaggcca tccgtaccca cctggtggat gtcaaggagg acggatcgat ctggctgaat
     3061 caggattact tcgattacgc caccgggctc agcatggtga aggacgacaa gtgggcggca
     3121 attttcggct tcccccgccg gacccccgag acggccatcg agcagcgcca cggggatctg
     3181 gcctatgcca ttcaacaggt gacggaggag atcgtcatga agatggcccg gacggcgcgg
     3241 cggctcaccg gggccgagaa tctctgtctc tccggcgggg tggccctgaa ctgcgtggcc
     3301 aacggcaagc tcctgcggag cggcgacttc gccggcatct ggatccaatc cgccgccgga
     3361 gacgccggcg gggcgctggg ggcggcctat gccatccatc acatgtacca ccagaaaccc
     3421 cggccccggg cggacggcgg
//
LOCUS       DAOSMF010000191         3393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_30336_length_3393_cov_20.552427, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000191 DAOSMF010000000
VERSION     DAOSMF010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30336_length_3393_cov_20.552427"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            218..391
                     /locus_tag="PLT63_10825"
     CDS             218..391
                     /locus_tag="PLT63_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99256.1"
                     /translation="MKDAASAHGQRAEIAIPARSAFDTDIAAVRLAQAKPVDYPLPCL
                     ELRRTIRKGQLPR"
     gene            553..1071
                     /locus_tag="PLT63_10830"
     CDS             553..1071
                     /locus_tag="PLT63_10830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOD99257.1"
                     /translation="MARIYARGRIYKIDVGALQPDPNQPRTFMAPGALTELTDSIRKY
                     GVLEPILFRRDEGGNLFIVAGERRVAAARRAGLTTIPAIYVESANHQEISLVENILRE
                     DLTAVEQAEALEQLMAERNYNQIQLSEIIGISQSHINEILAINRLPLNVRDQCRTNPN
                     IPKYILTEIAKD"
     gene            1084..1410
                     /locus_tag="PLT63_10835"
     CDS             1084..1410
                     /locus_tag="PLT63_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99258.1"
                     /translation="MTTKFEKYMAQQAAKEQPKQTTARLRKELVLIRQVDALNGKLVN
                     LRWQDWKIEDQEDLAVVLGDIRQTAENILQTMREGNEDEGEEEPNEESGTDDESRSGE
                     SKPRLS"
     gene            1516..1821
                     /locus_tag="PLT63_10840"
     CDS             1516..1821
                     /locus_tag="PLT63_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99259.1"
                     /translation="MSKRKTATGKQGENLFDYHHKSVTRLHIPPAGREAWGEEERRVA
                     VIFGPQYGPLTTRIVEEGIRIAARRGFDDVVFAAFSFDAQAQVTIQEVQDPAIKLHV"
     gene            2080..2832
                     /locus_tag="PLT63_10845"
     CDS             2080..2832
                     /locus_tag="PLT63_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99260.1"
                     /translation="MAKPLPTEPVLIQPVESPVICKPYYEPTEYWEYDRRQLRSLALV
                     AVLLFAVAACGENPTFTTAGTADKINVLRDPFQESCSSTETFSRRITGGSVKIIPVAT
                     YRIAGEVMSKRKYTQGWGAEIAPFDLALVWGMLTYPHVRKQLSIKHDNTRMAWFRMKG
                     DEPPVDHDYAMSHGANNHLIPASENIRMALDGQVKVNDKIALSGYLVDVEGRNHEREI
                     VMKTSLIRTDTDRGACEIIYVTRLQVGDKIYR"
     gene            2843..>3393
                     /locus_tag="PLT63_10850"
     CDS             2843..>3393
                     /locus_tag="PLT63_10850"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOD99261.1"
                     /translation="MKEQIKITAVIGSYRKGGIIDQTVDEILAAATARGGAASKIYLI
                     DRHVEFCRNCRICTQEEGERRGRCPQEDEMDAILDEIDESEAIILASPMNFGAVTAVM
                     KRFIERLICTAYWPWGQMAPKLRRREKKKRAVLVAASAAPAFLSRLMTPMVGRLKNAA
                     DVLGARTVGLLFVGLAARAQRQE"
BASE COUNT          817 a          925 c         1015 g          636 t
ORIGIN      
        1 taatgtaatc acaattactc aaaaaagttt cttgacccga gggggagctt tttcatatag
       61 ttccgtcatc ccgttgttat aaatgatcag taatgccgcc atgataaccc gcaaggacaa
      121 tctggcgcac ctccttggca agaattggac ttcaagttcg gctccggctt gtccggtttt
      181 ggaataagga gacatgatga ggacgaggag ggattttatg aaggatgccg cctccgctca
      241 cggccaacgg gcggaaatag cgatcccggc ccggagcgcc ttcgatacgg acatcgccgc
      301 cgtcaggctt gcccaggcta aaccggtcga ctatcccttg ccgtgcctgg aactgcggcg
      361 gacgatcaga aaaggtcaat tgccgaggta aatgacggcc gccccggtat ccccgcgccg
      421 gccggtggcg tggttcgccg ccatttagaa gcgcaggatc tgaatgagga ggtcaacatc
      481 tgctcctgac ggttgaccat cccagaaatt tagccaacct atgcacggtt tttcgagagg
      541 aggaacaggg agatggcgag gatctacgcc cgaggcagaa tctataaaat cgatgttggt
      601 gccctccagc ccgaccccaa ccagccccgg accttcatgg ccccgggggc actgacggaa
      661 ttgactgatt ccattcgcaa atacggggtt ttggaaccga tcctcttccg ccgggacgag
      721 ggcgggaatc ttttcatcgt tgccggagaa cgccgggtcg cggccgcccg gagagcgggg
      781 cttaccacca tcccggcgat ttatgtcgaa agcgccaatc accaggagat ttctctcgtg
      841 gagaacatcc tccgggagga tctgacggcg gtcgaacagg ccgaagcgct ggagcagctc
      901 atggcggaac gaaactacaa ccagatccaa ctaagcgaaa taattggaat atcccagtcg
      961 catatcaacg agattctcgc catcaaccgc ctgcccctaa atgtgcggga ccagtgccgc
     1021 acgaacccca acatccccaa atacattctc acggagatcg ccaaggacta gacggaagcg
     1081 ggcatgacga caaagtttga aaaatacatg gcccagcagg ccgccaagga gcagccgaaa
     1141 cagacaacgg cgcgcttgcg gaaggaactg gtcctgatca ggcaggtgga cgccctcaac
     1201 ggcaagctgg tcaatcttcg ctggcaggat tggaagatag aggatcagga agatctcgcc
     1261 gtcgtcctgg gcgacatccg ccagacggcg gaaaacatcc ttcagaccat gcgggagggc
     1321 aacgaagacg aaggcgaaga agagccgaat gaagagagcg gaactgacga tgaaagccgg
     1381 agcggggaaa gcaagccccg tctttcgtaa ttaccgttaa atatcaataa tatacatttc
     1441 attggaaatg ttgaaattat cggaattccg ataatttctt tgggcatggc tcataaccgc
     1501 tgaggaggca acttcatgag caaacggaaa acggcaacgg gaaagcaggg agagaatctc
     1561 tttgattacc accataagag cgtcacgcgg ttgcacatcc cgccggcggg acgggaggct
     1621 tggggggagg aggaacggcg ggtcgcggtg atcttcggcc cccagtacgg cccgctgacg
     1681 acgcggatcg tcgaggaggg catccgcatt gccgcccggc ggggattcga cgacgtggtt
     1741 ttcgccgcct tcagcttcga tgcccaagcc caggtgacga ttcaggaggt gcaggacccg
     1801 gcgatcaagc tccacgtatg aaaagacacg agctgatggc gtatttgaga tcgcaaggat
     1861 gtgaactcct tcgggaaggg gggcgcgtca ttcatggtgg cagaatcgag ccctccacaa
     1921 gcggtccgcg gcatacttaa atcaacggca tagaattcct tgatgcttcc aagaaggtat
     1981 ctctcggcag tctgctgact tatgagattg acgccgacgg tataggaacg cttggccgcg
     2041 ccgttgccgg ttaacggatt tcaagataaa ggctaagtaa tggcaaaacc tttaccaaca
     2101 gaacccgtcc tcatccagcc cgtggaaagc ccggtgatct gcaagcccta ttacgagccg
     2161 acggaatact gggagtacga ccgccgccag cttcggagtc tcgctctggt cgcggttctc
     2221 cttttcgccg tcgcggcctg cggggagaat cccaccttta ccactgccgg taccgccgac
     2281 aagatcaatg tcctgcgtga cccgtttcag gaatcctgct catccacgga gaccttttcc
     2341 aggcggataa ccggcggcag cgtgaagata atccccgtgg cgacctaccg gattgccggg
     2401 gaggtcatgt ccaaacggaa atacacccaa ggctggggcg cggagatcgc ccccttcgat
     2461 ctggccttgg tgtggggcat gctgacctat ccccatgtcc gtaaacagct ctccataaag
     2521 catgacaaca cccgcatggc atggttccgg atgaagggtg atgaaccacc ggtggaccat
     2581 gactacgcca tgtcccacgg agcgaacaat cacctcattc cggcgagcga gaatatccgg
     2641 atggccctgg acggccaggt caaggttaat gacaagattg ccctgtcagg ctatctcgtc
     2701 gacgtcgagg gccgcaatca tgaacgtgag atcgtcatga aaaccagttt gataaggacg
     2761 gatactgatc gcggggcctg cgagatcatc tatgtcaccc gcctgcaggt gggtgataag
     2821 atctaccgct gaagaggggg agatgaagga gcaaatcaaa ataacggcag tcatcggaag
     2881 ttaccgcaaa gggggcatca tcgatcagac cgtcgatgag atcctggcgg cggcgacggc
     2941 aaggggaggc gcggcgtcaa agatctatct gatcgaccgg catgtcgagt tctgccgcaa
     3001 ctgccgcatc tgcactcagg aggaggggga aaggcgggga aggtgtcctc aggaagacga
     3061 gatggatgcc atcctggacg agatcgacga gtcggaggcg atcatcctgg cctcgccgat
     3121 gaacttcggc gctgtcaccg ccgtgatgaa aaggtttatc gagcgtctta tctgtaccgc
     3181 ttactggccc tgggggcaaa tggcgcccaa attgaggcgc cgggagaaga agaaacgcgc
     3241 cgtgttagtg gcggcttccg ccgcgccggc attcttgtcc cggttgatga ccccgatggt
     3301 agggcgattg aagaacgccg cggatgtcct cggcgcccgg acggtggggc ttctttttgt
     3361 cggcctggcg gcccgcgccc agcgacagga gat
//
LOCUS       DAOSMF010000192         3367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_30629_length_3367_cov_22.272645, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000192 DAOSMF010000000
VERSION     DAOSMF010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30629_length_3367_cov_22.272645"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="PLT63_10855"
     CDS             complement(<1..203)
                     /locus_tag="PLT63_10855"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOD99262.1"
                     /translation="MPLFEGLAREEMEALAALLRRRSVRKGDVIFRKGDEGTNLYVIL
                     RGSVRITVPTALGDEIAVAVMKDG"
     gene            608..1066
                     /locus_tag="PLT63_10860"
     CDS             608..1066
                     /locus_tag="PLT63_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99263.1"
                     /translation="MQQRARTAMDRMVREIRMAGHRPTRSATSPPGAFLAANADSVQF
                     ILAGGDDSHAQQEAKAVTYGLFSREGIQLLGRKAAAGDVYQTIADHVQALRFTYYDSE
                     GNILAAPVAARSQIRHIQVTLTLRTVNPEPGRGHRTIILDSYVTPINPCW"
     gene            complement(1103..1825)
                     /locus_tag="PLT63_10865"
     CDS             complement(1103..1825)
                     /locus_tag="PLT63_10865"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOD99264.1"
                     /translation="MRRWTQLVVLPELFNTGYLIASREEAWSLGEEIPRGRTTVALSE
                     MAREKNLHIVAGLIEKSDDKLYNAAVLISPGGYVAKYRKLHLFNEEKLWFAPGDLGFA
                     VHDLGIGRIGIMICFDWFFPESMRVLALQGADVVCHPANLVLPFCQDAMITRCLENRV
                     YAITANRTGTETRAGKSFRYTGRSQITAPGAQVLCQADAGTEEIGTAELDLAVVRDKK
                     INAYNDLFSDRRTGHYAEILSS"
     gene            complement(2160..3353)
                     /locus_tag="PLT63_10870"
     CDS             complement(2160..3353)
                     /locus_tag="PLT63_10870"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HOD99265.1"
                     /translation="MSEAVIVEAVRSPGGRYKRGGLAATRGDDFAIEVLKGLLARVPQ
                     LKPEDVDDVIVGCSFPEAETGMNLGRVVAMGAGLPITTSGMTVNRFCSSGVQAIADAT
                     AKIRAGWSDVIIAGGCETMTHIPMGGGIFRPSPAWKFDGSMPNVYVSMGVTAENVAAN
                     HNISREDQDKFGLESNRRAYEAIKAGKFKEEIIPVTAYKYKVKNGKRIREAGIFDMDD
                     GVRWPTTIEDLARLKSPFKAGGSVTAGNSSQMTDGAAFTLLMTAEKAKELGLKPIAKL
                     AYYAVAGCKAEEMGVGPAYAIPKVLKMAGLTTKDIDVYEINEAFASQAIYSCRTVGIE
                     DRYWAGDINPNGGAIALGHPLGCTGAKLTAQLLHELKRRNAKRGIVSMCIGGGMGAAA
                     IYEML"
BASE COUNT          711 a         1053 c          897 g          706 t
ORIGIN      
        1 ccgtccttca tgacggccac ggcaatctca tcgcccagcg ccgtgggcac cgtgatcctt
       61 acggatcccc ggaggatgac gtaaaggttt gtcccctcat cgcccttgcg aaaaatcacg
      121 tctcccttcc tgacggagcg ccgccgcagg agcgcggcca gggcttccat ctcctcacgg
      181 gcaaggcctt cgaaaagtgg cattcgtctc aacagcgtaa ccagggacat atttgcctgt
      241 cacctttcat ctcttatagc tatgttttcc ttatgacaga atatttcttt ttccacaatg
      301 aagaaatgaa aattaacttt cgcattcaac tctttggatc ttaatattgg gcacacaata
      361 cttgcttcgt gtttgcgcgg ggatgacaga aaaatgtcaa atttcgtatt tgtttccggg
      421 ggaaagaaaa atctcggcct acgatatgtg acctatctca cgcagtgaag gtgatctgta
      481 tcacacttca accgcttatt tccattgact atttcgggat aatgaatcta atttgacaat
      541 cacaagcatt taaagggcct taaatatgcg ttacgatgac atcaacaatc aagatcagga
      601 ggcggcaatg cagcagcggg cgagaacggc catggataga atggtcaggg aaatacgcat
      661 ggcgggccac cgtcccacca gaagcgccac ttccccgccc ggcgccttcc tggcggcaaa
      721 cgccgacagc gtccaattca tcctcgccgg cggggacgac agccacgccc agcaagaagc
      781 taaagccgtg acgtacggcc ttttcagccg ggaggggatc cagctcctgg gacgcaaagc
      841 cgccgccggt gatgtatacc agaccatagc ggaccatgtc caggcgcttc gcttcaccta
      901 ctacgacagt gaaggcaaca tccttgccgc ccccgtcgcc gcgcgctcac aaatccgcca
      961 catccaggtg accctgaccc tacgcacggt caatccggaa ccgggccggg gacaccgcac
     1021 catcatcctc gactcttacg tgaccccgat aaatccatgc tggtgagatc agtcccggcg
     1081 cccccactcc ccaccacgac aatcaggagg acaggatctc ggcgtaatgc ccggtccggc
     1141 ggtcagagaa cagatcattg taggcattga tctttttatc cctgaccacc gccagatcca
     1201 gctccgccgt cccgatctct tccgtcccgg cgtccgcctg acaaagcacc tgcgcccccg
     1261 gagcggttat ctgactcctg ccggtgtagc ggaagctctt gccggccctc gtttccgtcc
     1321 cggtgcgatt ggcggtgatg gcatagacgc ggttttccag gcacctggtt atcatggcat
     1381 cctggcaaaa aggcaacacc agattggcgg gatggcagac cacgtccgcg ccctgcaagg
     1441 ccaggacccg catggattcc gggaagaacc agtcgaaaca gatcatgatg ccgatcctgc
     1501 cgatgccgag atcgtggacg gcaaaaccca ggtctcccgg cgcgaaccac agcttctcct
     1561 cgttgaagag atggagcttc cggtatttgg ccacgtagcc tccgggagaa atgaggaccg
     1621 cggcgttgta gagcttgtcg tcgctcttct cgataagacc cgccactatg tgcagattct
     1681 tctcccgggc catttcgctc agggcaactg ttgtccggcc ccgggggatt tcctccccca
     1741 ggctccaggc ttcctcccgg gaggcaatca aatatccggt attgaagagc tccggcagga
     1801 cgacgagctg ggtccaccgc cgcaaggagc gcccaggccc gggagagatt ctcctcaacg
     1861 acgccgaact ccggattgaa ttgaacaaaa cccgctttga ttttcataat ctttcccctg
     1921 ttccccggac ggacgccgac ccgaggcgat accgccgtca ccaccgttag gcacggtcat
     1981 atccggatat aaaacggacg tattttacga tacggatcta ataattgata accttctaaa
     2041 aagtcaaatt cttccgcccc tgttcaaggg tcgggccaaa acgaggatgg aggggaggaa
     2101 ggcccccccc attgctgagg agggccttta aggagggggt agacgttaga aaacttcttt
     2161 tacagcatct cgtagatagc cgcggcaccc atgccgccgc cgatgcacat ggaaacgatg
     2221 ccccgtttgg cgttccggcg cttcagctcg tggaggagct gggccgtcag cttggcgccc
     2281 gtgcatccca ggggatggcc cagggcgatg gcgccgccgt tggggttgat gtcgccggcc
     2341 cagtagcggt cttcgatccc caccgtacgg caggaataga tcgcctggga ggcgaaggcc
     2401 tcgttgatct cataaacatc gatgtccttc gtcgtcagac cagccatctt caataccttc
     2461 ggaatcgcat aggcaggacc gacgcccatt tcctcggctt tgcagcccgc cacagcgtag
     2521 taggccagtt tcgcgatggg cttcaggccc agttccttgg ccttttccgc cgtcatcagc
     2581 agggtgaacg ccgcgccgtc ggtcatctgg gaggagtttc ccgccgtgac cgacccaccg
     2641 gctttgaatg gcgatttcaa tctcgccagg tcctcgatgg tggttggcca ccggacaccg
     2701 tcgtccatat cgaaaatccc cgcctcgcgg atccgcttgc cgtttttaac cttgtattta
     2761 taggcggtga ccgggatgat ctcttccttg aattttcccg ccttgatggc ctcgtaagcc
     2821 cgacggttgc tctccaagcc gaacttgtcc tggtcttccc gggagatatt gtggttcgcc
     2881 gcgacgttct ccgccgtcac gcccatggaa acatagacgt tgggcatgga gccgtcgaac
     2941 ttccaggccg gactgggacg gaagatccct ccgcccatgg ggatgtgggt catggtttcg
     3001 caaccacccg cgatgatcac gtccgaccag cccgcccgga tcttcgccgt ggcgtccgca
     3061 atggcctgaa cgcccgagga gcagaaacgg ttgacggtca tgccggaggt ggtgatgggc
     3121 agcccggcgc ccatggcaac cacgcggcct aaattcatcc ccgtctccgc ttcgggaaag
     3181 gagcagccga cgatcacgtc gtccacatcc tccggtttca actgcggaac cctggccaaa
     3241 agacccttga ggacctcgat ggcaaaatca tcgcctctcg tcgcggccag accgcctcgt
     3301 ttataacgtc cgcccggact gcgtacggct tcaacaataa cagcttcact catgattttc
     3361 ctccttc
//
LOCUS       DAOSMF010000193         3362 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_30696_length_3362_cov_28.986090, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000193 DAOSMF010000000
VERSION     DAOSMF010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30696_length_3362_cov_28.986090"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            344..1531
                     /locus_tag="PLT63_10875"
     CDS             344..1531
                     /locus_tag="PLT63_10875"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HOD99266.1"
                     /translation="MTDYPQLILKTGRERSLLNGHPWLFSGAVAKTTSHPDAGDIVLA
                     CAAAGEPLALGFYHPRADIAFRMLTREVHIRVDGDFWRRRFRESAALRERILPVGDNA
                     WRLINAEGDGIPGLVVDCYGDYLVMGVGTVGVERRREEIVAALAAVSNPRGILERSEG
                     PARRLEGLEERRETVWGEEPPAVIAIRENGLVFEVDVHDGQKTGFFLDQRDNRRQVEE
                     LSRGLTVLNCFSYTGAFSVSAVRGGARRVVSVDSSEAANEAARRHLMVNGFDAAAHPV
                     IRSDVFRYLRNTEELFDFIILDPPAFAKNRREVARAARGYKDINLQSFRRLAPGGLLA
                     TFSCSNAVDEALFEKIVLGAARDAGRDLRLLRRFTAAPDHPTCLAHGEGRYLKGLLVS
                     VLA"
     gene            1651..2445
                     /locus_tag="PLT63_10880"
     CDS             1651..2445
                     /locus_tag="PLT63_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HOD99267.1"
                     /translation="MALVLVFFFSLLSILIFIGGCGGRKAYPPVPRDKKAAVKKTRPG
                     AVRKALPRMGYTIQAGAFSQAENAARLTELLKEQGLDATYFAAETGLFKVRIGNFPSR
                     ESARNQAEALQLLGLIEEYYLVAPEEYAVARAGELGEDYLRREIVKAAQSFIGVPYLW
                     GGTSAETGFDCSGLTMTVYQLNGIDLPRTSGDQFDTGEEQDDDDPLPGDLVFFATGKG
                     GKVSHVGVYIGAGRFIHAPGRGKNIRTDLMSNSYYQRRYLGARTYI"
     gene            2539..3270
                     /locus_tag="PLT63_10885"
     CDS             2539..3270
                     /locus_tag="PLT63_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD99268.1"
                     /translation="MLELKDLYFSVGDRNIIDGINYTFEKGKFYGITGPNGSGKTTLA
                     KLIMGINTPNAGEIRFGGKEISQWPITERAREGIAYSFQQPARFKGITFRDLLAMAAG
                     TDDEDMLLALLRRVGICSLSFLDKPVDAKLSGGEIKKIELATTIARNPRLAIYDEPDT
                     GIDLWTIQPMVRLLKREQKEHGTTTIVVSHNRAFLEAADIVLMINEGKLAYRGDLQGA
                     LPMLNDLSACNYRKCVGDRDVGCYR"
BASE COUNT          663 a          970 c         1069 g          660 t
ORIGIN      
        1 gaattcgtaa aaagcgccct agcccgccga ctgccgggcc tgtataaaag aagtaagtcg
       61 agtctgtgcc atgaaaccgg cccaaaaccg tcaaacacgt atctaaagaa tccaattagt
      121 tacgtgcgac ttacttagaa gtcatgatgg agacgacgaa gtaaaaagct ccagaggcaa
      181 ggcgcgcgaa tcctgaggaa tgagacgtac ttgtgcgtac gtcgcagtga cgaaggatga
      241 agcgcaacgc cgcatatgga ctttttacga tgtcgtcttt attgagatgc cgttttcccc
      301 ggcgctggca acatcccggc tatcttgtca aagggggcag ttattgactg attatccgca
      361 actcattctg aagaccggcc gggagcgttc cctccttaac ggacacccct ggttgttttc
      421 cggggcggtg gcaaaaacga cgagccatcc cgacgcggga gacatcgtcc tggcctgcgc
      481 ggcggcgggc gagcctctgg ccctgggctt ttatcatccc cgggcggata tcgccttccg
      541 gatgcttacg agggaggtgc atatccgggt ggacggcgat ttctggcggc ggcgctttag
      601 ggagtcggca gccctccggg aaagaatcct gcctgtcggc gacaatgcct ggcggctgat
      661 caatgcggaa ggggacggca tccccggcct ggtggtggat tgctacgggg actatctggt
      721 gatgggcgtc ggcaccgtcg gggtggaaag acgacgggag gagatcgttg ccgccctggc
      781 agcggtaagc aacccccggg gcatcctgga gcggagtgaa ggaccggcgc ggcgtctgga
      841 aggtctggaa gagcgccggg aaacagtctg gggcgaggaa ccgccggccg tcattgccat
      901 ccgggaaaac ggcctcgttt tcgaggtcga tgtccatgac ggtcaaaaga cgggtttttt
      961 cctcgatcag cgggacaacc gccgacaggt cgaggagctc agtcggggct tgacggtcct
     1021 gaactgtttt tcctataccg gggccttttc cgtctccgcc gtccgcgggg gggcacggcg
     1081 ggtggtttcc gtagattctt cggaggcggc caacgaggcg gcgcgacggc atctgatggt
     1141 gaacggcttc gacgccgctg cccatcccgt aatccggtcg gacgtttttc gctatctgcg
     1201 gaatacggaa gaactcttcg acttcatcat tctcgatccc cctgcatttg ccaaaaaccg
     1261 tcgggaggtc gcccgggccg cccggggcta caaggacatc aacctccagt ccttccggcg
     1321 gctggcgccg ggcgggcttc tggccacctt ttcttgttcc aacgccgtgg atgaggccct
     1381 gttcgaaaag atcgtcctcg gcgccgcccg ggacgccggg cgggacctcc gcctcctccg
     1441 ccggttcacc gcggccccgg atcacccgac ctgtctggcc catggcgaag gacgctatct
     1501 gaagggccta ttggtgagcg tcctggcatg atcaggcagg gagacgccag gaccggtcgg
     1561 gacggggggc gagcggacag cgactacccc agcggtttat ctcctatgat ctctccggtt
     1621 tccgggacgt catccggacg gcggcggcga atggcgttgg ttctggtttt ctttttctcc
     1681 ctcttgtcca ttctgatctt catcggcggc tgcggcggcc ggaaggccta tccgccggtc
     1741 ccccgggaca agaaggcggc ggtgaagaag acccggccgg gcgccgtgag gaaggccctg
     1801 ccccgcatgg gctacaccat tcaggccggg gccttttccc aggcggaaaa cgccgcccgg
     1861 cttacggaac tcttgaagga acagggcctg gatgccacct attttgcagc ggagacggga
     1921 ctgttcaagg tccgcatcgg gaattttccc tcccgggaaa gcgcccgcaa ccaagcggaa
     1981 gccttgcagc tgctggggct gatcgaagaa tattacctcg tcgctccgga ggaatacgcc
     2041 gttgccaggg ctggggaact gggggaggat tatctccggc gggagattgt caaggcggcg
     2101 cagagcttta taggtgttcc ctatctctgg ggggggacct ccgcggagac cgggttcgat
     2161 tgcagcggcc tgacgatgac ggtctatcag ctcaacggca tcgatctgcc ccgtacctcc
     2221 ggggatcagt tcgacaccgg cgaggaacag gacgatgacg atccgcttcc gggggatcta
     2281 gtttttttcg ccacggggaa gggcggcaag gtgtcccacg tcggggttta tatcggcgcc
     2341 ggccggttca ttcacgcccc gggccggggg aagaatatcc ggaccgacct gatgtcgaac
     2401 tcctattatc aaaggcgcta tttgggcgcc cgcacctaca tttgacgccc gggggcgcgg
     2461 ccgttataaa aaaattgaca ttggcggctt tttccatcat taaagattcg ctgttttgat
     2521 taatcgggag gtgtgaagat gctggaactc aaggatctgt atttctccgt gggggaccgg
     2581 aacatcatcg acgggatcaa ttataccttt gagaaaggaa aattctacgg gatcaccggc
     2641 cccaacggga gcggcaagac gacgctggcc aagctgatca tggggatcaa cacccccaac
     2701 gccggggaga tccgcttcgg cggcaaggag atctcccagt ggcccatcac ggaacgggcc
     2761 cgggagggga tcgcctacag tttccagcaa cccgcccgtt tcaaggggat caccttccgg
     2821 gacctcctgg ccatggccgc cggcacggac gatgaggata tgctcctggc gctccttcgc
     2881 cgggtcggca tctgttccct ttcctttctc gacaaaccgg tggatgccaa gctctccggc
     2941 ggggagatca agaagatcga actggccacc accatcgccc ggaatccccg gctcgccatc
     3001 tatgacgaac ctgatacggg catcgacctc tggacgatcc aacccatggt ccgcctcctg
     3061 aagcgggaac agaaggaaca cggcacgacc accatcgtgg ttagtcataa ccgggccttt
     3121 ctggaggcgg cggacatcgt cctgatgatc aacgaaggca agcttgccta tcggggggac
     3181 ctccagggcg ccctgcccat gctcaatgac ctgagtgcct gcaactatcg aaaatgcgta
     3241 ggagaccgtg atgttggatg ctatagataa ggaacttttg gcggctgtgg cggatctgga
     3301 aggactcccc aagggggcct tcaatatccg gaagaacggc cggttgctca agcgggaggt
     3361 tt
//
LOCUS       DAOSMF010000194         3338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_31019_length_3338_cov_22.446847, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000194 DAOSMF010000000
VERSION     DAOSMF010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31019_length_3338_cov_22.446847"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1291)
                     /locus_tag="PLT63_10890"
     CDS             complement(<1..1291)
                     /locus_tag="PLT63_10890"
                     /inference="COORDINATES: protein motif:HMM:NF028178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HOD99269.1"
                     /translation="MVKQDGFFSRTTAALGENFGLGRRAALAITIVAGLMIVFACWWF
                     FHSAPPKTIIITSGDKASLYHAVAEKYARILARDGVKLVILPSAGAVENLRRLADPSF
                     QVDIGFVQAGLDLGVNSDNLVSLGSVSYQPLLVFHRLPRAVTRLSQLAGKRLAVGREG
                     SGTRLLAMKLLAMNGIEPGGSTEFLAWEGEEAAAVLLKGELDAVFFMADAASSGTMRS
                     LLQAPGIGLADFTQADGYIRRIGYLHKLTMPRGAMNFGKDIPARDITLIGPTVELVAR
                     KNLHPALSDLLLAAAMEIHGRPALFQNRGEFPAALENELPISDDAKRYYKSGKTFLYR
                     YLPFWLASLINRILVVFVPLVIILIPGLKSIPALFHWRMKLRINRWYRQLLQVEQDAL
                     RRLDAGERDPLGRRIDAIEAEVHKMKVPAAYGDQFYVL"
     gene            2029..2832
                     /locus_tag="PLT63_10895"
     CDS             2029..2832
                     /locus_tag="PLT63_10895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOD99270.1"
                     /translation="MTEKKFEKGILYHLSIIDFKSDAQQPRKTLDTAALDELTASIKE
                     HGILEPVLFKEGDQGYVTVVAGERRIEAAKKAGLLTIPALYVDGNAAEIALVENMLRQ
                     DLTAVEEAEAMKRLMDEGGYNQEQLSNIIGKSRSTITDIIALTRLPQEIREECRGDHK
                     VSRAVLLEITRKKQDRAMRTEWNKYKEKVKKEQSGPQPRVVRAVTPEDMVKLVKKMEA
                     KLTDLSADEWTGEQKETFKESLAGLKDQIENLLANLERNDENAPSKRLA"
     gene            3010..>3338
                     /locus_tag="PLT63_10900"
     CDS             3010..>3338
                     /locus_tag="PLT63_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99271.1"
                     /translation="MKAIIQKVDLDTCLTALILGVDLSVDEVIVVKGEAGEEELSDPA
                     VVCIEAGGSGRSDLNNFDHHDPDRYFEPACRQAWRRQGRADSENPYPATGNFAAGRQA
                     WVRQGAE"
BASE COUNT          782 a          883 c         1031 g          642 t
ORIGIN      
        1 gaagcacata gaactggtct ccgtatgccg ccggcacctt catcttgtgg acctccgctt
       61 cgatggcgtc gatccttcgg cccagcgggt cacgttcccc ggcgtcgagc cggcggagcg
      121 catcctgctc cacctgcagg agctggcggt accaccggtt gatccgcagc ttcatccgcc
      181 agtggaacag tgccgggatg ctcttcaggc ccgggatgag gatgatgacc agggggacga
      241 aaacgacgag aatccggttg atgaggctgg ccagccaaaa gggcaggtag cggtacaaaa
      301 aggtcttgcc ggatttgtag tagcgtttgg cgtcgtcgct gatcggaagc tcgttttcca
      361 gggcggcagg gaattccccc cggttctgga agagcgcggg gcgcccgtga atctccatgg
      421 cggcggccag gaggaggtcc gacagggcgg ggtggaggtt tttacgcgcc acgagttcca
      481 cggtggggcc gatcagggtt atatcccggg cggggatgtc tttcccgaaa ttcatggccc
      541 cccggggcat cgtgagcttg tggagatagc cgatccggcg gatatagccg tcggcctggg
      601 tgaaatcggc caggcctatc cccggcgcct ggagcaggga gcgcatcgtc ccggaggagg
      661 cggcgtcggc catgaaaaac acggcgtcaa gctccccctt gaggagcacc gccgccgcct
      721 cctccccttc ccaggccagg aattcagtgc ttcctcccgg ttcgatgccg ttcatggcca
      781 ggagcttcat ggccagaagc cgggtgccgc tgccctcccg gccgacggcg agacgtttgc
      841 ccgcaagctg ggaaagccga gtgaccgccc gggggaggcg atgaaagacg aggagtggtt
      901 ggtaggagac gctgcccagg gagaccaggt tatcgctgtt gacacccaag tccagtccgg
      961 cctggacgaa gccgatatcc acctggaagg agggatcggc gagacgccgg agattttcca
     1021 ccgcaccggc ggagggcagg atgaccagct tcaccccgtc ccgggccagg atacgggcgt
     1081 atttctcggc aacggcgtga tacaggcttg ctttgtcgcc gctggtgata atgatggttt
     1141 tcggcggcgc ggagtgaaag aaccaccagc aggcaaaaac gatcatgagg ccggcgacga
     1201 tcgtgatcgc cagggcggcc cggcgcccca gaccgaaatt ctcacctaac gccgcggtgg
     1261 tcctactgaa aaaaccatct tgtttcacca tttgcacatt atgcttcgct ttcattccgg
     1321 gcgctttttc cccggagggt caggtatggg cggtccatga tggcaccggc cggatataaa
     1381 gtcaagtatg ttccagattg ccgatcccca tccctgctga tccgcttcgt ctttcccgat
     1441 agttgatgga cgggaaattg tctttgcgga acgtagggtt tattgtataa aatcccgcct
     1501 gttaggttaa ctattaaata aattcaatgc tggacaagtc gaaaatggca tccgatcatg
     1561 gccattcctt cctaacatgt cagtagggcg cggaatctca tacccgggcc acgactgcct
     1621 cccgggttcc gacggattca gtatcctccg gaggttattc cactacctct tttccgatgt
     1681 gattaaaata agcgtatatt tacgaatgtt tacaccgccg gtgcaccggc ggtgtttttt
     1741 cagggcaaaa aatggcagtc tatagccata attattatga ttctgacggg cctccatttt
     1801 gttgctgaat cagaaaggtc atgggccatt gacgcggctt ctccatttac tgactccgtc
     1861 gcccggcctg gctttcaaag gaaggtagga aaaaacatat agaaaacacc tagaaaaggg
     1921 ttgacaggac ggacattttt tcttactttc tttacgcaaa acaacggtgg ggatataaag
     1981 agctcagcgg cgatacgatg aaaaaagatg ataagaaacg aggacgatat gacagagaag
     2041 aagtttgaaa aaggcatatt ataccatctt tccatcattg attttaaaag cgatgcccaa
     2101 cagccgcgaa aaactctgga taccgcggcc ctcgacgaac tgacggcctc gatcaaggaa
     2161 cacggcatcc tggagccagt cctgttcaag gagggggatc agggctatgt gaccgtcgtg
     2221 gcgggggagc ggcggatcga ggccgccaaa aaggccggtc tgctgaccat cccggccctc
     2281 tacgtcgatg gcaatgcggc ggagatcgcc ctggtggaaa acatgctccg ccaggacctg
     2341 acggcggtag aggaagcgga ggcgatgaag cggcttatgg acgaaggggg ctacaatcag
     2401 gaacagttga gcaacatcat cggcaagtcc cggagcacga tcacggacat catcgccctg
     2461 acgcgcctgc cccaggagat tcgggaggaa tgccggggcg atcacaaggt ttcccgggcc
     2521 gtcctcctgg agataacgag gaagaagcag gaccgggcca tgcggacgga atggaacaaa
     2581 tacaaggaaa aggtgaaaaa ggagcagtcc gggccccagc cccgggtggt gcgggcggtg
     2641 accccggagg atatggtaaa gctggtgaag aaaatggaag caaaattgac cgacctcagc
     2701 gccgatgagt ggactgggga acagaaagaa acttttaagg agtccctcgc ggggctgaaa
     2761 gaccagatcg aaaacctcct ggcaaacctg gaaaggaacg atgaaaacgc tccgagcaag
     2821 agattggcgt gaggcgatca tggggtcgtc aaagtccgcc ttaaattgac cagaaaaata
     2881 gtcttataat atccaatatt tacaccgccg gtgcaccggc ggagcttttt gtggagaaaa
     2941 aacagccctt gatgaccata aatattgtgc gtccggcggg catccctgcc ctggcgaggg
     3001 aaattccaag tgaaagcgat aatacagaaa gtcgatctcg atacctgcct gacggccctg
     3061 atcctcggcg tcgatctttc ggtcgatgag gtgatcgtgg tgaagggcga ggccggggaa
     3121 gaggaattga gcgacccggc ggttgtctgc atcgaggcgg gcggcagcgg tcggtcggac
     3181 ctcaacaact tcgatcatca tgatccggac cgctatttcg agccagcctg ccgccaggcc
     3241 tggcggagac agggaagggc ggattccgaa aacccttatc cagcgactgg caacttcgca
     3301 gcgggtcgcc aggcctgggt gaggcagggg gcagaaga
//
LOCUS       DAOSMF010000195         3309 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_31371_length_3309_cov_19.046404, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000195 DAOSMF010000000
VERSION     DAOSMF010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31371_length_3309_cov_19.046404"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="PLT63_10905"
     CDS             complement(<1..314)
                     /locus_tag="PLT63_10905"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HOD99272.1"
                     /translation="MNAIFRKIVVIAFLFIFVPLFASTLWGFPPAAKPRISVLATGGT
                     IAGAAPVATQTIGYQAAVLPVGEVLASVPDLGRIADIHGEQVVQIASENMNPDIWMRI
                     ARR"
     gene            607..1623
                     /locus_tag="PLT63_10910"
     CDS             607..1623
                     /locus_tag="PLT63_10910"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOD99273.1"
                     /translation="MNESDESRHCLILTAGDLHREQVFFRAAGRRLAGQVIRPFRSDG
                     GGGKENKLPRPPRGTDDIGEVCTRRRKGGNQEEKKTEEIAEQNNKAEDGNWAGGGNQG
                     RPWLVFLHEGLGSVGQWRDFPAVICEETGLPGLVYDRWGYGNSEACEEKGNAEYLHHE
                     ALESLPQVLKHFGIEQAIFIGHSDGGSIALIFGAASPERTMGIITEAAHVFVEEVTLA
                     GIRVAITAYEQTDLPARLARYHGNRTDQVFRRWSGTWLAPAFRAWNIEELLPRIRCPV
                     LAIQGEDDPYGTPGQVESIVRRISGRAEGFIVPRCGHIPHHQARGEVAEAMKRFIHDI
                     LSIH"
     gene            1700..3079
                     /locus_tag="PLT63_10915"
     CDS             1700..3079
                     /locus_tag="PLT63_10915"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOD99274.1"
                     /translation="MIIAVLNGSPKGELSVTLQYVRYLQKIHPEHDYRIFSVARQIGK
                     LERDERAFGGIMDQVAAADAVIWSFPLYYCLVCSQYKRFIELIFAGGRQEAFRGKYAL
                     ALSTSVHFFDHTAHNYIHGICDDLGMNYFGHYSAEMYDLVRTRERARFLQCGENFLTA
                     VAGGIPTAPHFPPLHHSSFVYEPAPPLHDIDPGGRKILALCDEAREGANLKKMIQAWV
                     GNFSGGVEVIALTEIDIRGGCLGCCRCGIDNVCQYEGRDGYIDFYNEKVKKADIIVMA
                     GEIRDRYLSARWKQFFDRSFFNTHTPTLKDKQIAWIISGPLGQLPNLTEILSAYAQFH
                     GANTAGITTDEVGDSLRIDALLRELARRVVCLAEQGYVQTRDYLGVGGMKIFRDEVWG
                     KLRFVFQADHRYYKRHGFYDFPHKDIKTRRTNLMMTLLTKIPPFRKKFLKVLEKEMVK
                     PLKKVVADK"
     gene            3228..>3309
                     /locus_tag="PLT63_10920"
     CDS             3228..>3309
                     /locus_tag="PLT63_10920"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HOD99275.1"
                     /translation="MPCLDMKEGRVVKGVHFVDIRDAGDPV"
BASE COUNT          754 a          853 c          997 g          705 t
ORIGIN      
        1 cggcgggcga tcctcatcca gatgtcgggg ttcatgtttt cactggcgat ttgcactacc
       61 tgttccccat ggatgtcggc gatcctgccc aggtccggca cagaagccag gacttcgccc
      121 accggcagca cggcggcctg gtaaccaatg gtctgggtgg ccactggcgc cgccccggcg
      181 atggtgccgc cggtggccag gaccgagatc cggggttttg ccgccggtgg gaacccccag
      241 agggtggagg caaagagcgg aacgaagatg aaaagaaatg cgatcacgac aattttacga
      301 aaaattgcat tcatggtcat ttccgttgat atttttgaaa acggagccat tatggcaaat
      361 ttgtattttc cccgcaaggc cttttcctct ctatccactc cgaaccgggg caaattcagg
      421 ttgaagccat atgggttaaa tgttagaaat atcaaccacg gaacggccca caggtcctta
      481 ggtgtcggat cgttgggatt ttatatcgat ccacttctca taaggcacct tattatgcag
      541 taacttgagt gaagttgctc aagagcgaca accccatgga gaatgcggaa agagaatggt
      601 tccaccatga atgaatccga cgaaagccgg cattgcctga tcttaactgc cggggatctt
      661 caccgggagc aggtcttttt ccgggccgcc ggccggcgtc tggccgggca ggtcattcgc
      721 cccttccgga gcgacggcgg cggtggtaag gagaacaaac ttccccgtcc gccgagggga
      781 acagacgaca ttggggaagt gtgcacgagg cggcggaaag gcggtaacca agaggaaaag
      841 aaaacggaag aaatagcaga acaaaacaac aaggccgaag acggcaattg ggctggcggc
      901 ggtaatcaag ggcggccatg gcttgtcttt ctccacgaag gactggggag cgtggggcaa
      961 tggcgggatt tcccggcggt gatctgcgaa gaaaccggtc tgccgggact cgtttacgac
     1021 cgctggggct acggaaactc cgaggcctgt gaggaaaagg gcaatgctga gtatctccat
     1081 cacgaagctt tggaatccct cccccaggtc ctgaagcatt tcggtatcga gcaggcgatc
     1141 ttcatcggtc atagtgatgg tggatccatc gccctcatct tcggagcggc gtctccggaa
     1201 cggacgatgg ggataattac cgaggccgcc catgtgtttg tggaggaggt taccctggcg
     1261 gggatacgtg tcgccatcac cgcctatgag cagacggatc tccccgcgcg actcgcacgg
     1321 tatcacggca atcggaccga tcaggttttc cgcaggtggt ccgggacgtg gctggcgcct
     1381 gccttccggg cctggaacat cgaggagctg ttgccccgaa ttcgctgtcc ggtgctggcc
     1441 attcaggggg aagacgatcc ctacgggacg ccgggacagg tggaatccat tgtccgccgc
     1501 atctccggcc gggcggaagg tttcatcgtt ccccggtgcg ggcatattcc ccaccaccag
     1561 gcccgaggcg aggtggcaga ggcaatgaag agatttattc atgatatctt atccatacac
     1621 tgagcaggga aggggatcat ggtcgttgag tttaacggcg ggcaacggca attactccgg
     1681 aaaaatgagg tgaatcaacg tgatcatagc ggtgttgaac ggcagcccca aaggggagtt
     1741 aagtgttacc ctccagtatg tacgctatct gcaaaagatc catccggagc atgactatcg
     1801 gatcttttcc gtcgcccgac agatcggaaa actggagcgg gatgaacggg ccttcggcgg
     1861 catcatggac caagtcgccg ccgccgatgc agttatatgg tcctttcccc tctattattg
     1921 cctggtctgc tcccagtaca aacgcttcat cgaattgatc ttcgccggcg gccggcagga
     1981 agcctttcgg ggaaaatatg ccctggccct ttccacttcc gtgcattttt tcgatcatac
     2041 ggcccacaat tacattcatg ggatatgtga cgatctggga atgaattatt tcggacatta
     2101 ttccgcggag atgtatgatc tggtccggac tagggaacgg gcgcgtttcc tccagtgcgg
     2161 cgagaacttt ctcaccgcgg tggccggggg gatcccgacg gcgccgcact tcccgcctct
     2221 gcaccatagt tccttcgttt atgaaccggc cccgccgctt cacgacattg atcccggcgg
     2281 caggaagatc ctggcgcttt gtgacgaagc acgtgaaggc gccaatttga agaaaatgat
     2341 ccaggcctgg gtgggaaatt tctccggggg ggtggaggta atcgccttga cggaaatcga
     2401 catcaggggg ggctgcctgg gctgctgccg gtgtggaatc gacaatgtat gccagtacga
     2461 gggccgggac ggctatatcg atttctacaa cgaaaaggtt aaaaaggcgg acatcatcgt
     2521 catggcgggg gaaatccggg accgttacct gtcggcgcgg tggaagcagt ttttcgaccg
     2581 ttcctttttc aacacccaca ccccgaccct gaaagacaag caaattgcct ggattatctc
     2641 cggtcccctg ggacagcttc ccaatctgac ggagatcctg tcggcttatg cccagtttca
     2701 cggggccaat acggccggga taacaaccga cgaggttggg gattcgctgc gaatcgatgc
     2761 cctgcttcgg gaactggccc ggagagtcgt gtgcctggcg gagcagggct atgtgcagac
     2821 tcgggattat cttggcgtgg ggggcatgaa gatcttccgg gacgaggtct ggggaaagct
     2881 ccgtttcgtc tttcaggcgg atcatcgcta ctataagcgc cacgggtttt atgattttcc
     2941 ccataaggat atcaagaccc gccgaacgaa tctgatgatg accctcctga cgaagattcc
     3001 cccgttccgg aagaaatttc tcaaggtact ggaaaaggaa atggtcaaac cgttgaaaaa
     3061 ggtcgtggca gacaaatgag accgggccgg gtaaaaggcg gccttgggcg tgtatagccg
     3121 tcttccgtga tagctgcccg agatgtccgt taaaaggtcc tgctcaatct tctatatcag
     3181 aataatagtc ataaagaaag gataattaaa tgagagaaat aaaaatcatg ccctgtctgg
     3241 acatgaaaga ggggcgggtg gtcaaggggg tgcacttcgt ggatatccgc gatgccggag
     3301 atcctgtgg
//
LOCUS       DAOSMF010000196         3303 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_31447_length_3303_cov_19.010160, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000196 DAOSMF010000000
VERSION     DAOSMF010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31447_length_3303_cov_19.010160"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="PLT63_10925"
     CDS             complement(<1..554)
                     /locus_tag="PLT63_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOD99276.1"
                     /translation="MKKIRILIADDHPIVRAGFKLVISDTQDMTVADEAGNGQEVLNL
                     IRKKDYDIVLLDISMPGRNGLEVLKDLKAEKPKLPVMILSIYPEEQYAVRALRAGASG
                     YMTKASAPNELIAAIRKISQGGKYISASLAEKLTEYLDEDANKPLHEKLSDREYQVML
                     LIASGKTVSDIADELCLSVKTIST"
     gene            complement(551..>1536)
                     /locus_tag="PLT63_10930"
     CDS             complement(551..>1536)
                     /locus_tag="PLT63_10930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HOD99277.1"
                     /translation="RRIIFANDGVQTVFGWKARDIISMSTRILYRSDEDYEAMARALY
                     SALEKKRTVNLEFPCQHRDGRGIECMVTAARIGASLVEKNIVITYEDITDRKLAEMEL
                     ANSREQLRNLTAHLQSIREKERSHIARELHDELGQLLTALNTEIIMLGKKIPPEHTAL
                     IERTQSLSSLIDMTMQTLKRIYMDLRPGMLDHLGLTAAIGWQAEEFQKRTGIHCHVRF
                     APEEIVLDPDLTTALFRIFQETLTNVSRHAEASRVNVNLRATDREIQLTVKDNGRGIT
                     REQMNKPNSFGLLGIRERAEYWRGEVTIKGKPGRGTTVSVTIPLEQKGAPS"
     assembly_gap    1537..1636
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1637..2148)
                     /locus_tag="PLT63_10935"
     CDS             complement(<1637..2148)
                     /locus_tag="PLT63_10935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOD99278.1"
                     /translation="MDASLLKELKEIRRNVEEIRKLETERVFEEGLFRTLEYSSLMGI
                     YVVQDGIFRFVNQHTSNYWGYAREELLGMPSMNIVHPEDRRIVREGAIAMLKGTRFSP
                     YVFRTIAKDGSIRWITETVTTIHYQGKRAVLGNSMDITEQINARNKLAELEALEASIL
                     VAIPHAVIGL"
     gene            complement(2344..>3303)
                     /locus_tag="PLT63_10940"
     CDS             complement(2344..>3303)
                     /locus_tag="PLT63_10940"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOD99279.1"
                     /translation="VEDEWIVGDQICRNLRSFGYDSQNVAASAVEAFALIEEAKPDLV
                     LMDIVLPGELDGIEAAERIRSRYDLPVIFLTAHTDGDFIFRAKQTRPFGYLIKPFNKM
                     ELHTNIEMALHRHQLEKELQDCKKKLDLTLQGVIDALTEITEMRGPHSAGHHQRVKKL
                     AIALAAELGMNGFQAEGVGLAASVYDIGLINVPMEVIQDTSRLTGIKLSLYRTYPTIG
                     YGILKKIDFSWPIADIVLQHREYYDGTGFPRGMKGEEILQEARILAVADALEELTSHR
                     IYQRGLPQEEALTVIRNHSGSRYDPEVVAACLRLFNEKGFRTE"
BASE COUNT          532 a          895 c         1025 g          751 t
ORIGIN      
        1 taggtgctga tggtcttgac gctcaggcac agttcgtcgg cgatgtcgga cacggtcttc
       61 cccgaggcga tgaggagcat cacctggtat tcccggtccg agagcttttc gtggagtggt
      121 ttgttcgcgt cctcgtccag atactcggtg agtttttccg ccagagaggc gctgatgtat
      181 tttcccccct gggaaatctt gcggatcgca gcgatgagtt cgttgggggc gctggccttc
      241 gtcatgtagc ccgaggcccc ggcccggagg gcccggaccg cgtactgttc ctccggatag
      301 atgctgagga tcatgacggg cagtttgggc ttttccgcct tcaggtcctt caggacctcg
      361 aggccgttcc ggcccggcat ggagatatcg agaaggacga tgtcgtaatc tttttttctg
      421 atcagattga gtacttcctg accatttccc gcctcatcgg ccacggtcat gtcctgggtg
      481 tcggagatga cgagtttgaa tcccgcccgg acgatggggt gatcgtcggc gataagaatc
      541 ctgatcttct tcatgacggc gctcctttct gctccaaggg gatggtgacg ctcaccgtcg
      601 ttcccctgcc gggcttgccc ttgatggtca cctcgccgcg ccagtactcc gcccgttccc
      661 ggatgcccag gagtccgaag gagttgggct tgttcatctg ctcccgggtt attcctctgc
      721 cgttgtcctt tacggtgagc tggatctcac ggtccgtggc ccggaggttc acgttgaccc
      781 gggaagcctc ggcatgtcgg gaaacattgg tcagggtctc ctggaagatc cggaacaggg
      841 cggtggtcaa atcgggatcg aggacgattt cttccggggc aaaacggaca tgacagtgga
      901 tacccgtccg cttctgaaat tcttcggcct gccagccgat ggcagcggta aggccaagat
      961 gatccagcat ccccggacgc aggtccatgt agatccgctt gagggtctgc atggtcatgt
     1021 cgatgaggct tgagagggac tgggtccgtt cgataagggc ggtatgttcc ggggggatct
     1081 tcttccccag catgatgatc tcggtattca gggcggtgag gagttgacca agctcgtcgt
     1141 ggagttctcg agcgatgtgg gaccgctctt tttcccgaat ggactggagg tgggccgtga
     1201 gattccggag ctgttcacgg gagtttgcca gctccatttc ggccaacttg cggtccgtga
     1261 tgtcctcgta ggtgatgacg atgttttttt ccaccagact ggcgccaatc cgggcggcgg
     1321 tgaccataca ttcgatgcct cgcccgtccc ggtgctggca ggggaactcc aggttaacgg
     1381 tccgcttctt ctccaaggcg ctgtaaagcg cccgggccat ggcctcgtaa tcctcgtcgc
     1441 tgcgatagag gatccgggtg ctcatgctga tgatgtcccg ggccttccag ccgaatacgg
     1501 tctggacgcc gtcgttggcg aagatgatcc gccggtnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnttga ggccgatgac ggcgtgggga atggcgacga ggatggacgc
     1681 ctccagggcc tccagttccg ccagcttgtt ccgggcgttg atctgttcgg tgatgtccat
     1741 ggaattgccg aggacggccc gtttcccctg gtagtggatc gtcgtcaccg tctcggtgat
     1801 ccagcggata ctcccgtcct tggcgatggt ccggaagaca taaggagaga accgggtccc
     1861 cttgagcata gcgatggctc cctcccggac gatacgccgg tcctcggggt ggacgatgtt
     1921 catggagggc atcccgagga gttcttcccg ggcatagccc cagtaattgg aggtatgctg
     1981 gttgacgaac cggaagatcc cgtcctggac cacgtagatg cccatgaggg aactgtattc
     2041 cagggtccgg aagaggcctt cctcgaagac ccgttccgtc tccagtttcc tgatctcttc
     2101 gacgttgcgg cggatttcct tgagttcctt cagcaagctg gcgtccatgg gttcctttcc
     2161 gtcccggtga tgtgcacata tgtaattata ggttactgcc cttgttatgt caaacatttc
     2221 cgaagtcgta ttgatggtaa agggagtcgt gggaggcggt gatggtcggg tggtccgcta
     2281 atggcgttcc ccggagcggg gatttcggga cagcggagga gtccggtggt ccggcgggac
     2341 gggctattcc gtccggaagc ccttttcgtt gaacagccgg aggcaggccg ccaccacttc
     2401 cggatcgtaa cgggagccgc tgtggttcct gatcaccgtc agcgcttctt cctgggggag
     2461 tccgcgctgg tagatccggt gtgacgtcaa ctcctccagg gcgtcggcga cggccagtat
     2521 ccgtgcctcc tggaggatct cctcgccttt catcccccgg gggaagcccg tgccgtcgta
     2581 gtattcccgg tgctgcagga cgatgtcggc gatgggccag gagaagtcga ttttcttgag
     2641 gatcccgtag ccgatggtgg gataggtacg gtagagggaa agcttgatcc ccgtcagcct
     2701 gctggtgtcc tgaatcacct ccatggggac gttgatcaac ccgatgtcat aaacggaggc
     2761 cgccaggccg accccctcgg cctgaaatcc gttcatgccc agctccgccg ccagggctat
     2821 ggccagcttc ttcacccgct gatgatggcc ggcgctgtgg ggtccccgca tctcggtgat
     2881 ttcggtgagg gcgtcgatta ccccttggag ggtcaggtcg agttttttct tgcagtcttg
     2941 gagctccttc tccaactgat ggcggtggag ggccatctcg atgtttgtgt ggagttccat
     3001 cttgttgaag ggcttgatga gatagccgaa agggcgggtc tgcttggccc gaaagatgaa
     3061 gtccccgtcg gtgtgggccg tgaggaagat caccggcagg tcgtagcggg agcggatccg
     3121 ctccgccgcc tcgatgccgt ccagctctcc ggggaggacg atgtccatga ggaccagatc
     3181 cggtttcgcc tcctcgatca gggcgaaggc ctccaccgcc gaggcggcga cgttttggga
     3241 atcatagccg aacgacctta gattgcggca tatttgatcg cccacgatcc attcgtcctc
     3301 cac
//
LOCUS       DAOSMF010000197         3299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_31501_length_3299_cov_20.286683, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000197 DAOSMF010000000
VERSION     DAOSMF010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31501_length_3299_cov_20.286683"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1223)
                     /locus_tag="PLT63_10945"
     CDS             complement(<1..1223)
                     /locus_tag="PLT63_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HOD99280.1"
                     /translation="MMFKGKQRLFSFLTVMAIMLFLMVPMAFAGEADIVLPDLSKVTF
                     MDGALSGMLILNLGLAVCIIGMAFGWMQYSQTKALPAHQAMLDVSQTIWETCKTYVLQ
                     QGKFLAALWILIAICIVYYFGVLSHMEAGDIMVVLVCSILGILGSYGVAWFGIRINTV
                     ANSRAAFASMSGEPLNIVNICLRSGMSVGLLLVSIELFFMILILAYIPSELAGPSFIG
                     FAIGESLGASALRICGGIFTKIADIGSDLMKIIFHLPEDDPKNPGVIADCTGDNAGDS
                     VGPTADGFETYGVTGVALIAFLALALADNPLMGGKLVVWIFAMRILMILTSLASYFVN
                     DSVSKSMFSGPKVFNFEHPLTNLVWITSIVSIAVTFAASYVLLSDLPHVGGGIALWLA
                     LSIIISCGTIAGALIP"
     gene            complement(1223..1816)
                     /gene="pth"
                     /locus_tag="PLT63_10950"
     CDS             complement(1223..1816)
                     /gene="pth"
                     /locus_tag="PLT63_10950"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOD99281.1"
                     /translation="MFLIVGLGNPGRAYEGTRHNAGFLVIDRLSQDLEAPVEQKKFTA
                     LIGRAQIGAAKVMLVKPQTFMNLSGESVRRIFEYYQLEQTEEVIVVHDDLDLPFPALR
                     LKAGGGHGGHKGLLSVIHHLGSPDFLRVRVGIGKPVVKEAIERHVLDPFTREEAKLLP
                     AVVTTAAAAVSKIVSSGIQAAMNQYNGSTINNFSEEV"
     gene            complement(1864..2511)
                     /locus_tag="PLT63_10955"
     CDS             complement(1864..2511)
                     /locus_tag="PLT63_10955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HOD99282.1"
                     /translation="MEAIALRASARKISGKGPARRARAHGLIPAVFYNPKAEAIMLTV
                     SAADMKEFFKKNEDNAFINLRIEVNGTSLERMSIVKDVQRNPLTGQILHIDFYEISMD
                     HKLTADVALQFEGTPMGVELGGELQILKREVSLSCLPSVLPEYINLDISALKIGEALR
                     VKNIPAMEGITILEPDEAILAMVAVTREAMRSESEEEATASKEPEVLKQKAADAE"
     gene            complement(2521..>3299)
                     /locus_tag="PLT63_10960"
     CDS             complement(2521..>3299)
                     /locus_tag="PLT63_10960"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOD99283.1"
                     /translation="IIQSTCPPVNVTLMELLIMIDAMKRASAYRITAVIPYYGYARQD
                     RKVAPRAPISAKLVADLIATAGANRVLSMDLHAGQIQGFFNIPVDNLFATPVLLDYIK
                     EYYHDNTVIVSPDTGGVVRARAFATRMGVGLAIIDKRREGPNEAQVMNIIGNVNGRRV
                     VVLDDLIDTAGTVVQAAKALKEAGAVEVSVCCTHPVLSGPAMERLENSDIKEIIVTDT
                     IPLHDGAKACKRVKILSVAGLLSEAVRRIYYDDSVSSLFI"
BASE COUNT          750 a          914 c          936 g          699 t
ORIGIN      
        1 gggatcaggg cgccggcgat ggtcccgcaa ctgatgatga tggaaagggc cagccagagc
       61 gcaatgccgc cgcccacatg gggcaggtca gaaaggagaa catagcttgc agcaaaggtg
      121 acggcaatgg aaacgatcga ggtgatccag accagattgg tcagggggtg ctcgaagttg
      181 aataccttcg gaccgctgaa catggacttg cttaccgagt cgttgacaaa gtaggaggcc
      241 agggaggtga ggatcatgag gatacgcatg gcaaagatcc agacgaccag tttgccgccc
      301 ataagggggt tgtcggccag ggccagggcc aggaaggcga tgagggccac gcccgtgacg
      361 ccgtaggtct cgaaaccgtc cgccgtggga ccgacgctgt cgccggcatt gtcacccgta
      421 cagtcagcga tgacgccggg gtttttcgga tcgtcttcgg gaaggtggaa gatgatcttc
      481 atgaggtcgg agccgatgtc ggcgatcttg gtaaagatac cgccgcagat gcggagggcg
      541 ctggcgccga gggactcgcc gatggcgaag ccgatgaagc tgggaccggc caactcactg
      601 gggatgtagg cgaggatgag gatcatgaag aagagctcga tgctgacgag caggagaccg
      661 acgctcatgc cggagcggag acagatgttg acgatgttga gcggctctcc gctcatggat
      721 gcgaaggcgg cccgggagtt ggccacagtg ttgatccgta taccgaacca ggccacaccg
      781 taggagccga ggatccccag gatggagcag acgagaacga ccatgatgtc accggcctcc
      841 atatgggaaa gcacgccgaa gtaatagacg atacagatcg cgatcagtat ccacagggcg
      901 gcaaggaact tgccctgctg caggacgtag gtcttgcagg tctcccagat cgtctgggag
      961 acgtcgagca tggcctggtg ggccggcagg gccttcgtct gggagtactg catccagccg
     1021 aatgccatac cgatgatgca tacggcgagc cccagattca ggatcagcat cccgctcagg
     1081 gccccgtcca tgaacgtgac cttggacagg tcgggcagga cgatatccgc ctcaccggca
     1141 aaagccatgg gaaccatcag aaacagcatg atggccatga ctgtcaaaaa agaaaacaaa
     1201 cgctgtttac ccttaaacat cattaaacct cctcagaaaa attatttatg gtgctgccgt
     1261 tatattggtt catagccgcc tgaatcccgg aagatacgat cttgctcacc gctgctgcgg
     1321 ctgtagtaac aaccgccggc aggagtttcg cttcttctct ggtaaaggga tcaaggacat
     1381 gtcgctcgat cgcttccttt acaaccggtt ttccgatgcc cacccgcacc ctcaagaaat
     1441 ccgggctgcc caagtgatga atgaccgaca acagtccttt gtgcccccca tgaccgccac
     1501 ccgccttcag cctcagggct ggaaagggca gatcgagatc atcgtgaacg actatgactt
     1561 cttccgtctg ctccagttgg taatattcaa agatccggcg cacggactca ccgctcaggt
     1621 tcatgaatgt ctggggcttg acaagcatga ccttagcggc gccgatctgc gccctgccga
     1681 tcaaggcagt aaattttttt tgttccaccg gcgcttccag gtcttgagag agacgatcta
     1741 tgaccaggaa gccggcatta tgacgcgtcc cctcataagc ccggcccgga ttgccaagcc
     1801 cgacgatcaa aaacatctcc gtccatccct ttgacaaccc cgaattattt ccgcaaagca
     1861 atcctattcc gcatccgcgg ccttctgttt caagacttcc ggctctttgg acgccgttgc
     1921 ctcttcctct gattccgatc tcatggcttc cctggtgacc gctaccatgg ccaggatggc
     1981 ctcatcgggt tccaggatgg ttatcccttc cattgcggga atattcttta cccttaatgc
     2041 ctcaccgatt ttgagggcgc tgatatcgag attgatatat tccggaagga cggaaggcag
     2101 acacgaaagg gatacctctc tcttcaaaat ctgcagctcc cctcccagct caacccccat
     2161 gggcgtaccc tcgaactgca gggcgacatc ggcggtcaat ttatgatcca tactgatttc
     2221 gtagaaatcg atatgaagaa tctgtcccgt aaggggattc cgctgcacat ccttgacaat
     2281 cgacatccgc tccagagatg tgccgttaac ttctattcgg aggtttatga aggcattgtc
     2341 ttcgtttttc ttgaagaatt ccttcatatc ggcggcactg acggtcaaca tgatcgcctc
     2401 cgccttcgga ttgtaaaaaa cggccggaat cagcccgtgt gcgcgagcac gtcgggcggg
     2461 gccttttcct gagatcttgc gggcagacgc cctcaatgct atggcttcca ttaatgcctc
     2521 ctaaataaat agtgaactca ccgaatcgtc gtagtatatc ctacgcaccg cctcgctgag
     2581 gagtccggct acggaaagga tcttcacgcg tttgcatgcc tttgccccgt cgtgaagcgg
     2641 gatggtgtct gtcacgatga tctccttgat atcggaattc tccagacgtt ccatagcggg
     2701 tccggagagg acgggatggg tgcaacatac ggatacttcc accgctcccg cctccttgag
     2761 ggccttcgct gcctgaacga cggtaccggc ggtatcgatt agatcgtcga gcacaacgac
     2821 ccgccttccg ttaacattgc cgatgatgtt catcacctgg gcctcgttgg gtccttcccg
     2881 gcgcttgtcg atgatggcca gtcccactcc catccgagtg gcaaaggccc tggcccggac
     2941 cactccccct gtatccggag agacgatcac ggtgttgtca tgataatatt cttttatata
     3001 atcgagaagt acgggggtgg caaagagatt gtccacgggg atattgaaga aaccctgaat
     3061 ctggccggca tggagatcca tggaaaggac ccgattcgcc ccggcagtgg caatgaggtc
     3121 cgccacaagt ttggcggaga tcggcgcccg gggcgcgact ttcctatcct ggcgggcgta
     3181 accatagtag gggatgacgg cggtgatccg ataggccgaa gcccttttca tggcgtcaat
     3241 catgatcagg agctccatga gggtgacatt cacgggcggg cacgtcgatt ggatgataa
//
LOCUS       DAOSMF010000198         3266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_31912_length_3266_cov_25.679228, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000198 DAOSMF010000000
VERSION     DAOSMF010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31912_length_3266_cov_25.679228"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..1366)
                     /locus_tag="PLT63_10965"
     CDS             complement(65..1366)
                     /locus_tag="PLT63_10965"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOD99284.1"
                     /translation="MIRFVTILVMGIALWAGAVTAVEAPLIRDGEELTLERCIEIAVH
                     NQPEIIQYLYMTRANEADLGKALSGYYPQLDAKSIFNSYNLANKTDDPYPPINNLYGY
                     KYGDTNLTLTQKIYDFGKREDNVAVARLNVDSSRMDMENRITAVVNTVKNAYYDVLKA
                     KLSRDIEVEVREQYRKQLEQAKLFFTAGKKPKYDVTNAEVYLSGAELKLIDGENELSK
                     AWVSLSKAMGYDGPANYTVKELHNLDKLRIAEQEALERAYGNRQDLQSLLLQKKAAER
                     SVSLARKDYFPSLDANAGYEFAGSQTPLAQGWNVGVGVTWNLFAGFATQQSVQKALAN
                     LKVTEAKIAAIKLQIRQEVQTSLLNMKKAEESITNAQLLVRQATENLELVNLRYQSGL
                     ATSVEVTTATVNFSQAKQTRVNAYYNYVTARANLEKAMGGR"
     gene            complement(1435..3030)
                     /locus_tag="PLT63_10970"
     CDS             complement(1435..3030)
                     /locus_tag="PLT63_10970"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF022449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOD99285.1"
                     /translation="MFSISIVSPSRYRRAATRSLRACFLLFFLLLMTLSALPARAAED
                     VTLRFLGAARSVGGSAILLETGKSKILIDYGLSMERGKAGESTTPSFDPAAIDFVLLT
                     HAHADHAGRIPLLYRQGFRGRVVGTAATKTLAGLTLKAGLTYESGGGALMYGPEDVET
                     MLRNYESLPYGCRRQLTGDVAIRLRDAGHILGSAMIEVWTGSGGKMRKIVFTGDMGNG
                     SIPLLREPEMITEADFIVIEATYGTSRRDTEGQEELFVREIRETLAAGGSVLIPAFSM
                     DRTQKVLFTIGRWQRRGLFPAVTPVYADSSLGGKITDAYRRHRNYLHPEVQREFDAGR
                     DPFQFPGLQKVNGAAALLAHEQRQAAIFVTSSGMLDHGNAPRHLKRMGGDPRNLLAIV
                     GWQAPGSLGEALLAGRRRVAISPFHIPGNGAPQSGLETEIRMRVKRFDIFSNHADACR
                     ILTWLSRFPKTKQVFVVHGEGENTWRLAGMIERNLGFPAIAPDGKERFSIAAPLPDYE
                     RLPDAEPCRGLTGSNGRDVPDGD"
BASE COUNT          656 a          957 c          898 g          755 t
ORIGIN      
        1 gccggccgcc atgccccgtt gccaatatga tcttgtcgaa cataagatat ttgaccgtcc
       61 ttccctatct cccccccatg gccttttcca ggttggccct ggcggttacg taattgtagt
      121 aggcgtttac ccgcgtctgt tttgcctggc tgaagttgac cgtggcggtg gtaacctcta
      181 ccgaagtagc tagacccgac tgataacgga ggttgaccag ttccagattt tccgtcgcct
      241 gccgtactaa aagttgggcg ttggttatcg attcttcggc ctttttcata ttgaggagcg
      301 atgtctgcac ctcctggcgg atctggagct tgatggcggc gatcttagcc tcggttacct
      361 tgagattcgc cagggccttc tgcacggact gctgggtggc aaaacctgca aagagattcc
      421 aggtaacccc cacccccacg ttccagccct gggccagggg ggtctggctg ccggcgaatt
      481 cataaccggc gttggcgtcc agagagggaa aataatcttt cctggccagg gatacggatc
      541 tttccgccgc cttcttctgc aacaggagcg actggagatc ctggcggttt ccatatgccc
      601 gctccagggc ttcctgttcc gcgatccgca gtttgtcgag gttatggagt tccttgacgg
      661 tataattggc cggaccgtca taacccatgg ccttgctcag ggagacccag gccttgctca
      721 gttcgttttc cccgtcgatg agcttgagct cggcgccgct gagatagacc tcggcgtttg
      781 tcacatcgta tttcggcttc ttgccggccg taaagaacaa ttttgcctgt tccaattgct
      841 ttcggtattg ttcccgcact tccacctcga tatcccggga tagtttcgcc ttcaggacat
      901 cgtaataggc gtttttgacg gtattgacca ccgcggtgat cctattttcc atgtccatcc
      961 gggaggaatc gacgttgaga cgggccacgg cgacgttatc ctcccgcttc ccgaagtcgt
     1021 agatcttctg ggtaagggtc agattggtgt ccccgtattt atagccatag agattgttga
     1081 tgggtggata ggggtcgtcg gttttgttgg ccaggttgta actgttgaag atcgatttgg
     1141 catccaactg gggatagtat cctgacaagg ccttgccgag atccgcttcg ttggccctgg
     1201 tcatatacaa atactgaatt atttccggtt ggttatggac ggcaatctcg atacagcgct
     1261 ccagggtcaa ctcctcgccg tcccggataa ggggcgcctc caccgctgta acggcgcctg
     1321 cccacagggc gatccccata accaagatgg tgacgaaccg aatcatacct cgcctctctc
     1381 caaaagcagt tatgcctatc ataccgggag ttcccttgtc aaagaaatgc gccctcagtc
     1441 gccatcagga acgtcccggc cgtttgatcc ggtcagaccc cggcagggct cggcgtcggg
     1501 caaacgttca tagtccggca acggcgcggc aatggaaaat cgctctttcc cgtccggagc
     1561 gatcgcagga aaacccagat ttcgttcgat cataccggcc agtctccagg tgttctctcc
     1621 ttcgccgtgg accacgaata cctgtttcgt cttggggaaa cgggacagcc aggtgagaat
     1681 ccgacaggcg tcggcatggt tggaaaagat gtcgaatctc ttcacccgca tccgtatttc
     1741 cgtctccagc cctgattgcg gcgccccgtt ccccggaata tggaaagggg aaatggccac
     1801 ccgccgcctc cctgccagca gcgcctcccc caggcttccc ggggcctgcc agcccacgat
     1861 ggccagaagg ttcctggggt cgccacccat cctcttcagg tggcgcgggg cgttgccgtg
     1921 atcgagcatg ccgctggagg ttacgaagat cgcggcctgg cgctgttcat gggccaggag
     1981 ggcggcggca ccgttcacct tttgtaatcc gggaaattgg aagggatccc gaccggcatc
     2041 gaattcccgt tgtacctcgg gatggagata atttcggtgc cggcggtaag cgtcggttat
     2101 cttgccgccc agagaactgt cggcatatac cggggtgacg gcggggaaca aaccacggcg
     2161 ctgccaccgc ccgatggtga acaggacctt ctgggtccgg tccatgctga aggcgggaat
     2221 caggacgctc cctccggccg caagggtctc ccttatctcc cggacaaaga gttcctcctg
     2281 gccttccgta tcacgccggc tggtcccata agtggcctcg atgacgatga aatcagcttc
     2341 agtaatcatt tccggctccc tgagaagggg gatgctccca ttccccatgt cgccggtaaa
     2401 gacgatctta cgcattttgc ctccggaacc cgtccagacc tcgatcatcg ccgaaccgag
     2461 gatatgcccc gcatcccgga gacggatggc tacatccccg gtaagctggc gccggcaacc
     2521 atagggaaga gattcatagt tcctcaacat cgtctctacg tcttcagggc catacattag
     2581 cgcaccgccg ccggactcat aggtcagccc cgccttcagg gtaagcccgg ccagggtttt
     2641 cgtggccgct gtaccgacca cccgaccccg gaaaccctgc cggtaaagta aaggtatgcg
     2701 cccggcatga tccgcgtggg catgggtcag caggacaaag tcgatcgcgg cgggatcgaa
     2761 ggacggcgtg gtactttcac cggccttacc ccgttccatg gacagaccgt agtcgatgag
     2821 tatttttgat tttcccgtct ccagaaggat ggcgctgccc cccacgctgc gggccgcgcc
     2881 gagaaacctc agggtaacgt cctccgcagc ccgggccggg agggcggaaa gcgtcatgag
     2941 aagcaggaaa aacagtaaaa aacatgcccg caaagatcgt gttgcggcgc ggcggtaccg
     3001 cgacggcgaa acaattgata tcgaaaacat ggctaattaa atcacagccg atttctcaat
     3061 gcaaccgtcc ctttgctcat accatccctg cggtacggtc atatctaaac gtttttccat
     3121 tttatcgtcg tgggccagaa tatagatcta tcatgtccac acttcttttt tgttgcatta
     3181 cacggcaaat atcattatat tgcagcttct ttcagatgtc atgatcagga cagtggcatt
     3241 gcattcccgt ggagagaaaa ggccct
//
LOCUS       DAOSMF010000199         3250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_32128_length_3250_cov_22.872613, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000199 DAOSMF010000000
VERSION     DAOSMF010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32128_length_3250_cov_22.872613"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1580
                     /gene="murJ"
                     /locus_tag="PLT63_10975"
     CDS             <1..1580
                     /gene="murJ"
                     /locus_tag="PLT63_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HOD99286.1"
                     /translation="EKSRENEKVARAAGVVGLATMLSRVFGFIRDMVVAGFFGAGLVT
                     DAFFVAFRIPNLLRRLLAEGSLTVSFVPVFTEYLKKRSRQEALELADVVFTSLSVVLV
                     VVSIAGVLFAPFIVAVMAPGFVKHDAQYELTVLLTRFMFPYIFFISLVALCMGILNSL
                     RHFAAPALSPVVLNISMILAAVLLRDFFSEPVFSLAVGVMVGGVLQLAMQWPFLLKMG
                     VRLRLNFRFRHPGLKRIGLLMMPAAFGAAIYQINVFIGTVLASLLPKGSVSYLYYADR
                     IVELPLGVFAIAVGTAALPSFSDQVARGEFGDFKKTISFSLRLILFITVPATVALLAL
                     REPILSVLFQRGAFDAAATVKTAEALLYYTLGLWAFSLIRVIVSAFYSLQDTKMPMKA
                     AIVALIVNVAASIMLMGPMRHGGLALATSLASAVNVLMLGIVLKKRIGSYLNREFYRS
                     LGKMSAASLVMWISILLVETIIPWRIAGPLDHRLLFLTVSVMSGLATYFAASWALKCP
                     EMVMVIRTVKAKLREKR"
     gene            1655..2935
                     /gene="serS"
                     /locus_tag="PLT63_10980"
     CDS             1655..2935
                     /gene="serS"
                     /locus_tag="PLT63_10980"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003218092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOD99287.1"
                     /translation="MLDIKFIRQNTELVRRKMLERGQEMDMTPFLDLDTRRRDILQEV
                     EKLRNDRNTVSKEIGEKKKKKEDAAELIARMGEVSARIKTLDEELKKVEEDLNSLVMV
                     IPNIPHETVEYGASSEDNPVVRTWGERPVFSFPPKPHWDIGEALGILDFERGAKITGA
                     RFTVYKGLGAMLERAIFNFMLDLHIFQHGYTEVLTPFMVNRASMTGTGQLPKFEEDLF
                     RVDVLDYFLIPTAEVPVTNIHRDEILNEKDLPIYYVSYSPCFRAEAGSYGKDTRGLIR
                     QHQFNKVEMVKFSRPETSYDELEKLTANAEEVLQLLKIPYRTVSLCTADLGFSSAKTY
                     DVEAWLPGQDAYREISSCSNFEDFQARRASIRFRREESGKIEFVHTLNGSGLAVGRTV
                     VAVLENYQQADGSVVIPEVLRPYMKGIDRIPAIG"
     gene            2963..3048
                     /locus_tag="PLT63_10985"
     tRNA            2963..3048
                     /locus_tag="PLT63_10985"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2997..2999,aa:Ser,seq:tga)
     gene            3064..3157
                     /locus_tag="PLT63_10990"
     tRNA            3064..3157
                     /locus_tag="PLT63_10990"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3098..3100,aa:Ser,seq:gct)
     gene            3171..3247
                     /locus_tag="PLT63_10995"
     tRNA            3171..3247
                     /locus_tag="PLT63_10995"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3205..3207,aa:Arg,seq:acg)
BASE COUNT          612 a          959 c          962 g          717 t
ORIGIN      
        1 aggaaaaaag cagggaaaac gaaaaggttg ccagggcggc gggggttgtc gggctggcaa
       61 cgatgctgag ccgggtcttc ggcttcatcc gcgacatggt cgtcgccggc ttctttggcg
      121 ccggcctggt taccgacgcc ttcttcgttg cctttcggat ccccaacctc ctgcgccgcc
      181 tcctggcgga aggctccctg accgtttcct tcgtccccgt attcactgaa tatttaaaga
      241 agcgttcccg tcaggaggcc ctggaactgg ccgatgtggt ctttacctcc ctctcggtgg
      301 ttctggtcgt cgtctccatc gccggcgtcc tattcgcgcc gttcatcgtc gccgtcatgg
      361 ccccggggtt cgtcaagcat gacgcccaat atgaactgac ggtcctgctg acccggttca
      421 tgttccccta tatcttcttt atctccctgg tggccctctg catggggatc ctcaattccc
      481 tgcgccattt tgcggccccg gccctgtcgc cggtggtctt gaacatctcc atgatcctcg
      541 ccgccgtcct gctgcgggac ttcttttccg aacctgtctt ctccctggct gtgggggtca
      601 tggtcggcgg ggttctccag ttggccatgc agtggccgtt tctcctcaag atgggggttc
      661 gtctccggct gaatttccgg ttccgccatc cgggtctgaa gcggatcggc ctcctcatga
      721 tgcccgcggc ctttggggcc gccatctatc agatcaatgt ctttatcggg accgttctgg
      781 cctccctcct ccccaagggg agcgtttcct acctctacta cgcggaccgc atcgtcgagc
      841 tgcccctggg ggtctttgcc atcgccgtgg ggacggcggc cctgcccagc ttttccgacc
      901 aggtggcccg gggggagttc ggggatttca agaagaccat ctccttttcc ctgcggctga
      961 tcctctttat caccgttccc gccaccgtgg ccctgttggc ccttcgggag ccgatcctct
     1021 cggtgctttt tcagcggggg gccttcgacg ccgccgccac cgtcaagacc gccgaggcgc
     1081 tgctctatta caccctgggg ttgtgggcct tttcgttgat ccgagtcatc gtttcggcct
     1141 tctattccct ccaggacacc aagatgccca tgaaggcggc gatcgtagcc ctgatcgtca
     1201 atgtcgccgc cagcatcatg ctcatggggc cgatgcggca cggcggtctg gccctcgcga
     1261 cctccctcgc ctcggcggtg aacgtcctca tgcttgggat cgttttgaaa aaacggatcg
     1321 gttcctattt aaaccgggag ttttaccgct ctttagggaa gatgtccgcc gcttccctgg
     1381 tgatgtggat ctccatcctg ctcgtggaga cgatcattcc ctggcggatc gccggccccc
     1441 tcgatcaccg tctcctgttt cttacggtga gcgtcatgtc cgggctggcg acatattttg
     1501 ccgcctcctg ggcattgaag tgtccggaga tggttatggt gatccggacg gtaaaggcca
     1561 agctccgcga gaaaagataa agaggttgac ttgaaacatc aaaagtgata ggcattttaa
     1621 gctgttatcg gaaaaatacc aaaggaatca agccatgctg gacatcaaat tcatccgcca
     1681 aaatacggaa ctggtaagaa ggaagatgct ggagcggggg caggaaatgg acatgacccc
     1741 gtttctcgat ctggacacgc gccgccggga catccttcag gaggtggaaa aacttcgcaa
     1801 cgaccgcaat accgtctcca aggagatcgg ggagaaaaag aagaagaagg aggacgccgc
     1861 ggaactgatt gcccgcatgg gggaggtttc cgcccgtatc aagaccctgg acgaggaact
     1921 gaagaaggtg gaggaggatc tcaacagcct ggtcatggtc atccccaaca tcccccacga
     1981 aacggtggag tacggcgcct cgtcggagga caacccggtg gtccggacct ggggggaaag
     2041 gccggtcttt tcctttccgc ccaagcctca ctgggatatc ggcgaagccc tgggcatcct
     2101 ggatttcgag cggggggcca agatcaccgg ggcccgattt accgtttaca aggggctggg
     2161 cgccatgctg gagcgggcca tcttcaactt catgctggat ctgcacatct ttcaacacgg
     2221 ctatacggag gtgctgacgc cgttcatggt caaccgggcc agcatgaccg gaacgggaca
     2281 gcttcccaaa ttcgaggagg atctattccg ggtggacgtg ttggattatt tcctgatccc
     2341 cacggcggag gtgccggtga ccaatatcca ccgggacgag atcctgaacg aaaaggacct
     2401 gcccatctat tatgtgtcct actcgccgtg cttccgggcc gaagccggtt cctacggcaa
     2461 ggacacccgg gggttgatcc gccagcacca gttcaacaag gtggagatgg tgaagttctc
     2521 cagacccgag acctcctatg acgagctgga aaagctgacg gccaacgccg aagaagtcct
     2581 tcagctcctg aagatcccct accggaccgt gagcctctgc acggcggatc tgggcttctc
     2641 ctcggccaag acctacgatg tggaggcctg gctgccggga caggatgcct atcgggagat
     2701 atcctcgtgc agtaacttcg aggatttcca ggcccggcgt gcttcaatcc gcttccgtcg
     2761 ggaggagtcg ggaaagatcg agttcgtcca taccctgaac ggatcggggc tggcggtggg
     2821 gcggacggtg gtggccgtcc tggagaatta tcagcaggcc gacggcagcg tcgtcatccc
     2881 cgaggtcctg cgtccataca tgaaagggat tgacaggatt cccgcaatag ggtaagggcc
     2941 gcctacaagt tatgacggta acggagggat ggccgagtgg tctatggcgg cggtcttgaa
     3001 aaccgttggg cgaaagtctc gcgggttcga atcctgctcc ctccgccaga aaagttcgat
     3061 cgcggagaga tggctgagtg gtcgaaagcg atcgcctgct aagcgattgt acgccctaaa
     3121 aggtgtaccg cgggttcaaa tcccgctctc tccgccattt ttatccatat gcgcccgtag
     3181 ttcagctgga tagagcgtca gactacgaat ctgaaagtcg cacgttcgaa tcgtgccggg
     3241 cgcgccaata
//
LOCUS       DAOSMF010000200         3201 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_32768_length_3201_cov_27.880801, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000200 DAOSMF010000000
VERSION     DAOSMF010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32768_length_3201_cov_27.880801"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="PLT63_11000"
     CDS             complement(<1..334)
                     /locus_tag="PLT63_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99288.1"
                     /translation="MKTSLRKLAAGPLLPVGIAALYCLVIGFGVYVIDYHPRGAIQNF
                     LLNNITWILRLNFLLMILGAVACGRDIAAAFRGLIIPAPAAPDAPDAPDAATDCSSAA
                     PTKAPAASA"
     gene            complement(315..2048)
                     /locus_tag="PLT63_11005"
     CDS             complement(315..2048)
                     /locus_tag="PLT63_11005"
                     /inference="COORDINATES: protein motif:HMM:NF014589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HOD99289.1"
                     /translation="MKSEREFVLGVWDGHDAGAALVRGGELVCAINEERLTRRKLEVG
                     FPSLAIGACLEFAGLTPRDVKYIAASTTDPAKTLTRLFPRLKEEYYLIRRRKLPPRTA
                     DPFKKAFKYRFTELAPNPVSIALSRRHLTGRLRALGFRDFRLSLVDHHAAHAVAAAAC
                     SGFTEALVITLDGVGDGLCGTVRRFAGGRLELLRALPAHTSLGIFFEHVTNLMNMREL
                     EDEGKVMALANYAYPIPDEENPLMDLIAATDGNIVSRYSATALHRELRRILWRYPSEQ
                     FAAMAQRVLEKCILALVRHYVDATGLRRIAAAGGVFSNIKMNMKIAAAGGVERIYVFP
                     HMGDGGLGIGAAMHLNHEAFGVAGYRLDDLYLGPHHSRAEILAACAAARQEGGALTWE
                     TVADAPQRAARFILDGEIILWFGGRMEIGPRALGNRSILARPDNNRIKDRLNVLLKKR
                     VWYQPFCPSILAEDGPPLLHANGQCLDDNRFMTMAFRVREEYLPLMAGVINIDGTCRP
                     QFVKEENPAYRELLLALKAELGYGVVLNTSFNIHGEPLVCTPAEALKMLRQTGIRYLF
                     LEDVLVENLAA"
     gene            complement(2067..2975)
                     /locus_tag="PLT63_11010"
     CDS             complement(2067..2975)
                     /locus_tag="PLT63_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99290.1"
                     /translation="MKKRSPGFDGGTISVMMMAAAVLLMLLTVAVPPAPAGPGPGANA
                     GPVKIIPKVTAGPSGRMIVEYEVRNAGSAPVYHLLVTTFLARDARRSDPYGQIAAGQA
                     WTSYRCDFDLGAMIPGDYIMVSRLSYDDHAGKPHLLYDFHPFMNRTGLPAGEKPPLAV
                     AVNAEALNVKSLGEATAKYRISLVNNHKGPVQAVVSFYLPDGITMDETDRFYELGPGG
                     TQADEAVVKLDAGLPPARYPYRAVVWYDFNGVQFSRLLQDEAPVEEKPVLLIVFAVAV
                     LAAIAAAGGWLWWRRRRGSGTVGSGL"
BASE COUNT          568 a          972 c         1123 g          538 t
ORIGIN      
        1 ctgctgaggc cgcgggagct ttagtgggag cggcggatga acagtcggtc gcggcgtcgg
       61 gagcgtcggg agcgtcgggg gcggccggcg ccgggatgat caggccccgg aaggccgcgg
      121 cgatgtcacg cccgcaggcg accgccccga ggatcatgag gagaaagttc aggcggagga
      181 tccaggtgat gttgttcagg agaaaattct ggatggcgcc ccggggatgg tagtcgatga
      241 cgtaaacgcc gaagccgatg acgaggcagt aaagcgccgc gatccccacc gggagcaggg
      301 ggccggcggc aagtttacgc agcgaggttt tcaaccagga cgtcctccag gaacaggtag
      361 cggatgccgg tctgccggag catcttcagg gcctcggcgg gggtgcagac caggggctcg
      421 ccgtggatgt tgaaactcgt gttcaggacc accccgtagc cgagctccgc cttcagggcc
      481 aaaagcagct cccggtaggc gggattttcc tccttgacga actgggggcg gcaggtgccg
      541 tcgatgttga tgaccccggc catgaggggc aggtactcct cccggacccg gaaggccatg
      601 gtcatgaagc ggttgtcgtc caggcactgc ccgttggcgt ggaggagcgg cggtccgtcc
      661 tcggcgagga tgctgggaca gaacggctgg taccagaccc gtttcttcag caggacgttg
      721 aggcggtcct tgatgcggtt gttgtccggg cgggccagga tgctgcggtt ccccagggcc
      781 cgggggccga tctccatgcg ccccccgaac cagaggatga tctctccatc caggatgaaa
      841 cgggccgccc gttgcggggc gtcggcgacg gtctcccatg tgagggcgcc gccctcctgc
      901 cgggccgcgg cgcaggccgc caggatctcc gcccgggaat ggtgggggcc caggtacaga
      961 tcgtcgagac ggtagccggc cacgccgaag gcctcgtgat tcaggtgcat cgccgcgccg
     1021 atacccaggc cgccgtcgcc catgtggggg aagacgtaga tccgctccac cccgcccgcg
     1081 gcggcgatct tcatgttcat cttgatattg gaaaaaaccc cgcccgcggc ggcgatgcgc
     1141 cgtagccccg tggcgtccac gtaatgccgg acgagagcga ggatgcattt ctccaggacc
     1201 cgctgggcca tggcggcgaa ctgctccgag ggatagcgcc agagaatcct gcggagctcc
     1261 cggtgcaggg ccgtggcgct gtagcgggag acgatgttcc cgtccgtcgc ggcgatcagg
     1321 tccatgaggg ggttttcctc gtcggggatg ggataggcgt agttggccag ggccatgacc
     1381 tttccctcat cctccagctc ccgcatgttc atgaggttgg tgacgtgctc gaagaagatc
     1441 cccagggagg tatgggcggg aagggcccgg aggagttcca gccgtccccc ggcaaagcgg
     1501 cggacggtgc cgcagaggcc gtcgccgacg ccgtcgaggg tgatcaccag ggcctcggta
     1561 aagccgctgc acgctgcggc ggccaccgca tgggcggcgt ggtgatccac gagggagagg
     1621 cggaagtccc ggaagcccag ggcgcgcaga cgcccggtga gatggcggcg gctcagggcg
     1681 atggaaacgg gattgggggc cagctcggtg aaccggtact tgaaggcctt cttgaaggga
     1741 tcggcggtgc gggggggcag cttccggcgc cggatgaggt agtattcctc cttgaggcgg
     1801 gggaagaggc gggtcagggt cttggccgga tccgtcgtcg aggcggcgat gtatttcacg
     1861 tcccgggggg tgagaccggc gaactccagg caggcgccga tggcaaggct ggggaatccc
     1921 acctccagct tcctccgggt gaggcgctcc tcgttgatcg cacagacgag ttccccgccc
     1981 cggaccaggg ccgccccggc gtcatggccg tcccagacgc ccaatacaaa ttcccgctca
     2041 cttttcataa gataaaaacg ctaattttaa agacctgacc ccactgtgcc tgacccccgg
     2101 cggcggcgcc accacagcca gccgcccgcg gcggcgattg ccgccaggac cgccacggcg
     2161 aagacgatga ggagcacggg cttctcctcc accggggcct cgtcctggag gaggcgggaa
     2221 aactggacgc cgttgaagtc ataccagacc acggcccggt agggataacg cgccgggggg
     2281 aggccggcgt cgagcttcac caccgcctcg tccgcttgcg tccctcccgg acccaactca
     2341 tagaaacgat ccgtttcgtc catggtgatg ccgtcgggaa ggtagaagct cacgacggcc
     2401 tggaccggac ccttgtgatt gttgacgagg gagatccggt atttcgccgt cgcctcgccc
     2461 agactcttta cattgagggc ctcggcattc acggccaccg ccagcggcgg cttttccccc
     2521 gccgggaggc ccgtccggtt catgaagggg tggaagtcat agagcagatg gggcttgccg
     2581 gcgtggtcgt cataggagag gcggcttacc atgatatagt cgccggggat catggccccc
     2641 agatcgaagt cgcagcgata ggacgtccag gcctggccgg cggcgatctg gccgtaggga
     2701 tccgagcggc gggcatcccg ggcgaggaag gtggtcacca agagatgata taccggcgcg
     2761 gagccggcgt tgcggacctc gtattctacg atcatccggc cggaggggcc ggcggtcact
     2821 ttggggatga tttttaccgg cccggcattc gcccccggtc ccggcccggc cggcgccggc
     2881 gggacggcaa ccgtcaggag catcagcagg accgctgcgg ccatcatcat gacagatatg
     2941 gttccgccat caaaacctgg agatcttttc ttcattgccc ttaaaacctc gtcataaaaa
     3001 ttgctggttt tgtactatag cctccggagc aggtcaaggt gatttcccga aactaaaaaa
     3061 gcggcggcgc ccccggggcc gccttttccg gatagaaacc gcggatgaaa cccgtaatgt
     3121 ccttgaggat cttcttgttt cccgtaagcg tcagggcctt gtggtaatgg cgcatggccc
     3181 ggaaccagta atcctcctgc t
//
LOCUS       DAOSMF010000201         3178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_33087_length_3178_cov_22.809798, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000201 DAOSMF010000000
VERSION     DAOSMF010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33087_length_3178_cov_22.809798"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..1138
                     /locus_tag="PLT63_11015"
     CDS             152..1138
                     /locus_tag="PLT63_11015"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HOD99291.1"
                     /translation="MTTCRWPLLLAAAVSRQIASGPRAEKAPPASARSPLNPKIAPRI
                     APELRPNRGRPPCSGKSRDIESGLDEFGPRKFCNSLGRFISIDLLGGSMDKPQSLNLY
                     TYCLNNPVRFTDPTGMVVNWDDSKKTQKKGETTARSNLERKFHNYVDKLLRSKDAADV
                     AKGQKLQAQYNRLDAAQETFHVVKDKASDASAGELEYRGQPGNLYICMKGNAGSYGAL
                     SDIQKLIHEFTHGEQFLDRKLGFFQSGLSYQYDLTDEAEAFIAGFEVEPLSPAQKGNS
                     FLNGLSQQIPFGLTNVVNYLGGSKSPYQRMVRGPNNVPTTIPSNSPVGIYAH"
     gene            1128..1841
                     /locus_tag="PLT63_11020"
     CDS             1128..1841
                     /locus_tag="PLT63_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99292.1"
                     /translation="MRTKLIMVCIAAFIQIAAAESMCSSIRKGYLCGSTNYQWIGKTE
                     IAPEITIDLRHKKTILQIQTDEFGAYEINLDPGNYFVLRAYDANGKDIEFLLDQPRCI
                     KVREGKKNPSFSFMLKPPSIQGGKQHQAFSQYDCESSDYGFLQGIVGYKTIGDFVRAP
                     GVTIEFKGKGKTVFVQTDEYGEYLERLEPGKYSLISIRDGAGQEIELFTGQLRRTKVR
                     KKDCARFYIELKQIQLPAP"
     gene            1871..2098
                     /locus_tag="PLT63_11025"
     CDS             1871..2098
                     /locus_tag="PLT63_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99293.1"
                     /translation="MPNQAGWGQAFDPARLWPVRIVAGTTAVTANTSTASGAVLFGLD
                     YDNYEANGNIAAVTRTLRTGAYGTPYTMDFG"
     gene            2098..2235
                     /locus_tag="PLT63_11030"
     CDS             2098..2235
                     /locus_tag="PLT63_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99294.1"
                     /translation="MPDREVVREDSLGFQPQVGRADPQSVPTLKGWGEIATSIHPQVS
                     P"
     gene            2293..2745
                     /gene="tnpA"
                     /locus_tag="PLT63_11035"
     CDS             2293..2745
                     /gene="tnpA"
                     /locus_tag="PLT63_11035"
                     /inference="COORDINATES: protein motif:HMM:NF033573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HOD99295.1"
                     /translation="MGSTLTRLVYHIVFATKDRFPSLTSEVRERVYPYIGGIVRGEGG
                     HLLAIGGIADHVHLLTVLPAAEAVAAIIQKVKGHSSKWINEERLLKAKFGWQRGYGAF
                     TVSHSAVSRVKHYVETQEVHHQRMTFEEEFVALLERHGVAYDPRYLWS"
     gene            2816..>3178
                     /locus_tag="PLT63_11040"
     CDS             2816..>3178
                     /locus_tag="PLT63_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99296.1"
                     /translation="MKPQAVPLDRFAIGAAADNRRGDDPITARTRAAGGGGDLPSSLA
                     LVVSRATNRLGGAGYYHDVLGNLVQMPSPNSQDSLFMTYLDQGHIGTLTDAAGVVWKY
                     YYDADGKRRIKVKTAGGAT"
BASE COUNT          747 a          856 c          924 g          651 t
ORIGIN      
        1 gacataacgg gagcattatg tcaccttccc ggcggctgga gcccggtccg gcgccgggcg
       61 gcgcccggcc gaatccgcga cccgcccttt ctttagctgc caacagctgc taaaataaag
      121 gcgtcttccc gccccttccc ggcccggttc tttgacaact tgccgctggc cgcttctgct
      181 ggcggccgcc gttagccgcc aaatcgcatc ggggcctcgc gccgaaaaag cgccgcccgc
      241 atccgcccgg agtccgctta acccaaagat cgccccgagg atagcgccgg aattacgacc
      301 gaatcgcggc cgacctccct gttccggcaa atccagagat atagaatcgg gattggatga
      361 attcggacca cggaaatttt gcaacagctt gggacggttc atatccatcg acctattggg
      421 aggttcgatg gataaacccc aatctttgaa cctatacact tattgtctga ataatcccgt
      481 cagattcacc gacccgaccg gaatggttgt caattgggat gactcgaaaa agactcagaa
      541 gaaaggagag acgacagcac ggtctaatct ggagcggaaa tttcacaatt acgttgacaa
      601 gctccttcgt agcaaggatg cggcggatgt agctaaaggt cagaaattac aggcacaata
      661 caaccgactt gacgcggccc aagagacctt ccacgtggtc aaggacaagg cttctgacgc
      721 cagcgccgga gaattagagt accgcggcca acccggaaat ctgtacatct gcatgaaagg
      781 caatgctggc agttacggcg ccttgtcgga tattcaaaaa ttgatccatg aatttacgca
      841 cggagagcaa ttccttgacc gaaagcttgg gttttttcaa tcaggattgt cctatcaata
      901 tgatctcaca gatgaagcag aagctttcat tgctggattc gaggtggaac cgctgtctcc
      961 ggctcagaaa gggaactctt ttctgaatgg tttaagtcag caaatcccat ttggcctgac
     1021 taatgttgtt aattatcttg gtggatccaa atcaccctat caaagaatgg tcagaggtcc
     1081 aaacaatgtc ccaaccacga ttccttcgaa ttctccggta ggtatatatg cgcactaaac
     1141 tcatcatggt atgtatagca gcatttatcc agattgctgc tgccgagtcg atgtgttctt
     1201 cgataaggaa agggtattta tgcggaagta ccaattacca gtggattggg aaaacagaaa
     1261 tagctcctga aataacgata gatcttcgac acaaaaaaac aatattacaa atccaaacag
     1321 atgaatttgg agcctatgag atcaatttgg accccggaaa ttactttgtc ttgagagcgt
     1381 atgatgccaa tggcaaagat atcgagttct tacttgatca gccaagatgt atcaaagtaa
     1441 gagagggcaa gaaaaaccct tcgtttagct ttatgttgaa accaccgtcc attcaaggtg
     1501 gcaagcaaca tcaggctttc tctcagtatg actgtgaatc gtctgattat ggctttcttc
     1561 aaggaattgt tgggtataaa acgattggcg atttcgttag agctcctgga gttactatcg
     1621 agtttaaagg gaaggggaaa acggtttttg tccagaccga tgagtatggc gagtatttag
     1681 agagattaga gccggggaaa tattctctca tcagcatccg tgacggagcc ggccaagaga
     1741 ttgagctgtt tacaggacaa ttgcgccgca cgaaggtaag aaaaaaagac tgtgcccgtt
     1801 tctatattga actgaaacaa atacagttac ccgctccgtg aagttatcgg ttccgccgag
     1861 acgggctcag gtgccgaacc aggcgggctg ggggcaggcg ttcgacccgg cgcggctctg
     1921 gccggtgcgg atcgtggcgg ggaccaccgc ggtgaccgcc aacacctcca ccgccagcgg
     1981 cgccgtgctc ttcgggctgg actacgacaa ctacgaggcc aacggcaaca tcgcggcggt
     2041 gacgcggacg ctccgcaccg gcgcctacgg gaccccgtac accatggatt tcggctaatg
     2101 ccggaccgcg aagtggtccg ggaagacagc ctggggtttc aaccccaggt ggggcgtgcc
     2161 gatcctcaat ccgttcccac cctgaagggg tggggggaga ttgccacctc gatccatccc
     2221 caggtttccc catgagcacc gccagcgcca tggtacaatc cggccatcgc atcttcgcac
     2281 gccggaggcc ccatgggcag cacgctgacc cggttggtct accacatcgt gttcgccacc
     2341 aaggaccgct tcccgtcgct gacttcggaa gtccgcgaga gggtgtatcc ctacatcggc
     2401 gggatcgtcc ggggcgaggg cggtcatttg ctggcgatcg gaggcattgc cgatcatgtg
     2461 cacctgctga cggtgctgcc ggcggccgag gccgtggcgg cgatcattca gaaggtgaag
     2521 gggcattcgt cgaaatggat caacgaggag cgcctcctga aagcaaaatt cggttggcag
     2581 cggggctacg gggcgttcac agtgagccac tcggcggtgt cgcgggtgaa gcactatgtg
     2641 gaaacccagg aagtgcacca tcaacgcatg accttcgagg aggaattcgt ggcgctgctg
     2701 gagcgtcacg gggtggcgta tgatccgcga tacctttgga gctgaagacc atgtcgtcat
     2761 ctcctgcacc ccttcagggt gcgggggact tgggggtggg ataccggacc tggggttgaa
     2821 accccaggct gtccccctcg atcgcttcgc gatcggggcg gcggcagaca atcggcgcgg
     2881 ggatgatccc atcacggcgc gcacgcgggc cgcggggggc ggcggcgacc tgccgagctc
     2941 gctggcgctg gtggtgagcc gggcgacgaa ccggctgggc ggcgccgggt attaccacga
     3001 cgtgctgggg aacctggtgc agatgccgtc gcccaacagt caggactcgc tgttcatgac
     3061 ctacctcgac caggggcaca tcgggacgct caccgacgcg gccggggtgg tctggaaata
     3121 ctactacgac gcggacggca agcggcggat caaggtgaag accgccggcg gcgcgacc
//
LOCUS       DAOSMF010000202         3171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_33202_length_3171_cov_25.481386, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000202 DAOSMF010000000
VERSION     DAOSMF010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33202_length_3171_cov_25.481386"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..588
                     /locus_tag="PLT63_11045"
     CDS             172..588
                     /locus_tag="PLT63_11045"
                     /inference="COORDINATES: protein motif:HMM:NF018391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HOD99297.1"
                     /translation="MIVYDLRCDRGHKFEGWFQDRQAFDAQKQGRLISCPICGSVEAE
                     IVPAAVNVVGRDSRPVTNDNKGEISPMRYFEQLNRQLERCFDNVGDRFAEIAFRIHYG
                     EEERRNIRGTTTKQEEEALREEGIPFVKIPLPKFDS"
     gene            608..2917
                     /gene="cysS"
                     /locus_tag="PLT63_11050"
     CDS             608..2917
                     /gene="cysS"
                     /locus_tag="PLT63_11050"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOD99298.1"
                     /translation="MKPPSSHILATIGNTPLVEIRHLSDNPQVRIFAKMECFNPGGSI
                     KDRTALAMIEGAERRGELTKDKVILEATSGNTGIGLALVAAAKGYRLCLTMSEAASEE
                     RKKILRAMGAELHFTPANLGTDGAIEAAYLMMRENPDRYFGTDQFNNPDNVRAHYEGT
                     AEEIWRQTDGQVTAVVVALGTTGTAMGLSRRLKEYNPEVRIIGVEPYLQHKLQGLKNM
                     KESYRPGIFDRNLLDEKVNILDEDAFETARRLAREEGLLVGMSSGAAMHVGLRKAREM
                     TSGVLVVIFPDSGERYLSTDLFTEKGEMTLRLYNTLTRQKQFFKPLRSEEVRIYSCGP
                     TVHDVPHIGSYRRFVVSDLLRRYLEYKGCRVRHVTNIVDLADRSIRGAERTGADLVTY
                     TARCTEAFLRDIGKLNIKEDEMYPRASEHVDDMIRMVEKLVGKGYAYEKLRSVYFDIS
                     KFNEYGSLSNIDLRKVQHGRTSDLDDYEKDSPVDFTLLKRSTLSELKKGIYYKTRWGS
                     VRPSWHLECAAIAMKFLGEAFDIHASGADITFPHCENVLAIGKASTGRGMANYWLSTE
                     LVMIDGKKMSRSLGNVLTMEDLERKGYEGREIRFFLLGAHYRKPLNFSWGALETAKNT
                     VRKLNRFVQRLLRFRPGMGCPDTDQWIYDVRHSFIDAMDDDLNISGALASLFDFINKV
                     SPPLEQGCFTLEERDRLLDALQGLDGVLGVMTFAEERIDGEVLTLVEKRAALRKEMRW
                     AEADAVRAELAALGIEVYDTPQGSFWRLR"
BASE COUNT          737 a          839 c          932 g          663 t
ORIGIN      
        1 cgcgactcct gcgtcccgaa cgcagtgccc taccaggctg ggccacatcc cgacacggcg
       61 gataaatata aaattaccgc cggaatgtcc aacaatattt tcggcgatgt agtgactatt
      121 gcaactgttt gattttattt gcaacgatat ttaatgtgcg agggaagatc cgtgattgtc
      181 tatgatctgc gctgtgatcg cggtcataaa ttcgagggct ggtttcaaga ccggcaggct
      241 tttgatgccc agaaacaggg acggctgatt tcctgtccaa tctgtggcag tgtggaggcg
      301 gagatcgttc cggcggcggt gaacgttgtg ggccgggatt cccgtccggt gaccaacgat
      361 aataaagggg aaatttcccc gatgagatat tttgagcagt tgaatcggca actagaaagg
      421 tgcttcgaca atgtcggcga ccggtttgca gagattgcct tcaggatcca ttacggcgag
      481 gaggagcggc gcaatatccg cgggaccaca accaaacagg aagaggaggc cctccgcgag
      541 gaagggatcc ctttcgtgaa gattcccctg ccgaaattcg acagttaagc taaggtcaac
      601 tccagccgtg aaaccaccct cttcccatat tttagccacg ataggaaata cgccgctggt
      661 ggagattcgc cacctcagcg acaatccgca ggtgcgtatc ttcgccaaaa tggaatgttt
      721 caatcccgga ggctccatca aggatcggac agccctggcc atgatcgagg gtgccgaacg
      781 gcggggagaa ttaacgaaag acaaggtgat tctcgaggcg acaagcggca atacgggtat
      841 tggcctagcc ctggtggccg ccgccaaggg ctatcgcctc tgccttacca tgtcggaggc
      901 ggccagcgag gagcggaaga agatcctccg ggccatgggg gccgaacttc acttcacccc
      961 cgccaatctt ggcaccgacg gcgccatcga ggcggcctat ctaatgatga gggaaaatcc
     1021 cgaccgatat ttcggcacgg accagttcaa caatcccgat aatgtccggg ctcactatga
     1081 gggaacggcg gaggagatct ggcgccaaac cgacggtcag gttacggccg tcgtcgttgc
     1141 cctggggacc acaggaacgg ccatggggct gtcgcggcgc ctcaaggaat acaacccgga
     1201 ggtccggatc atcggcgtgg aaccttattt gcaacacaaa ctgcaaggtc taaagaacat
     1261 gaaggaatcc tatcgtcccg ggatattcga ccggaatctc ctggacgaga aggtcaacat
     1321 tctcgatgag gatgccttcg aaacagcccg gcgactggcc agagaagagg ggctcctggt
     1381 ggggatgagt tccggcgccg ccatgcatgt aggcctccgg aaggcccggg aaatgacgag
     1441 cggggtcctg gtggtaatct ttcccgacag cggcgagcgc tatctgagta cggatctgtt
     1501 caccgaaaag ggtgaaatga cgttgcggct gtacaacacc ctgaccaggc agaagcagtt
     1561 cttcaagccc ctgcgctccg aagaggtacg gatctattcc tgtggtccca ccgtccatga
     1621 cgtgccccat atcggcagct accgccgttt cgtcgtctcc gatctgctca ggagatattt
     1681 ggagtacaag ggctgccgag tccgccatgt aaccaatatc gtcgatctcg ccgaccgatc
     1741 gatccggggc gccgagcgga ccggcgcgga tctcgttacg tacacggccc ggtgcacaga
     1801 ggctttcctt agggatatcg gcaagttgaa catcaaagaa gacgagatgt acccccgggc
     1861 gagcgaacat gtggacgaca tgatccgcat ggtggaaaag ctggtgggga agggctatgc
     1921 ctatgaaaaa ctccggtccg tttatttcga catctccaag ttcaacgaat acgggagtct
     1981 gtccaatatc gacctgagga aggttcagca cggccggact agtgatctgg acgactatga
     2041 gaaggacagc ccggtggatt tcaccctcct gaaaaggtcc accctgagtg aactaaagaa
     2101 gggtatttac tataagaccc gctggggaag cgtccggccc agctggcacc tcgaatgtgc
     2161 cgccattgcc atgaaatttc ttggtgaggc atttgatatc catgccagtg gcgccgatat
     2221 caccttcccc cactgcgaaa atgtcctggc catcggcaag gcgtccaccg gccggggcat
     2281 ggccaactac tggctgagca cggaactggt gatgatcgac ggcaagaaga tgtcccggtc
     2341 gctgggcaat gttctgacca tggaggatct ggaaagaaag ggttatgaag gtcgggagat
     2401 acgcttcttt cttctgggtg cccactatcg caagcccctg aatttttcct ggggtgctct
     2461 ggagaccgcc aaaaacaccg tccggaagct gaaccgtttc gtccagcgct tactccgttt
     2521 ccgccccggt atgggttgtc ccgatacgga ccagtggatc tatgacgtcc ggcacagctt
     2581 tatcgatgcc atggatgacg acctgaatat ttcgggtgcc ctggcctccc tcttcgactt
     2641 tatcaacaaa gtcagcccgc ccctcgaaca gggatgcttc accctcgagg aaagggaccg
     2701 cctcctcgat gcgctccagg gactggacgg ggtgctgggg gtcatgacct ttgcggagga
     2761 acggatcgac ggggaggttc tgacgttggt ggaaaaacgg gcggcactgc gcaaggagat
     2821 gcggtgggcg gaggccgacg ccgtccgtgc cgagcttgcc gctttgggga tcgaagtcta
     2881 tgacacccct cagggcagtt tctggcggct ccgttgacaa gccggacatg ctcgcatcgg
     2941 caatggtcgg ataattataa tgagtggctt aatgttcata aaaagttctt gacaaaataa
     3001 ggcaataaat ctaagtttcc caattcgcat tttcggctcg gattagcgac gccggcaata
     3061 tcgcgataag ttctttgaat tacgtgttaa agtgttattt gctggcgtag ctcaacaggt
     3121 agagcagctg atttgtaatc agcaggttgc gggttcgagt cccatcgcca g
//
LOCUS       DAOSMF010000203         3128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_33810_length_3128_cov_18.042304, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000203 DAOSMF010000000
VERSION     DAOSMF010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33810_length_3128_cov_18.042304"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="PLT63_11055"
     CDS             <1..737
                     /locus_tag="PLT63_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99299.1"
                     /translation="YPAGAVVFKDKRILAVQTFHAAYVQDLRAQAPVSIGHYVLEGSK
                     PGAAAAAAWTAHRVLPLNIDGYGRLLGRTVRTTNMLSAGMTAAAPLVTKSGRRYRFVV
                     LAEPDLNILDYVLLEVGNPSLKAMNKLNQAIYEACSYISGDLMKKDFIVSKTAFSPAE
                     YGNRPLDFIRKCGFADREWEKTPSVLVLRSTVMTPYLAGEEDFGAYFERLRAILGRIL
                     SRIEAQGAGDGPISGGRGMPIWPANS"
     gene            complement(715..1164)
                     /locus_tag="PLT63_11060"
     CDS             complement(715..1164)
                     /locus_tag="PLT63_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99300.1"
                     /translation="MSMVGKNVDALCTRCGLTLAHIVLYEVGGVIARVKCKTCGAEHK
                     YRGAKVRDRKGETGGDRTRRTGTRAAEESRQAEAVETQRWLQRHNELGKNTPIRPYRM
                     TDRYQRGEVIEHPTFGIGFVEKILEDQRMDVLFKGSLKRMVMSLPAK"
     gene            complement(1450..>3128)
                     /locus_tag="PLT63_11065"
     CDS             complement(1450..>3128)
                     /locus_tag="PLT63_11065"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HOD99301.1"
                     /translation="VTLLKGKSNYLCAEKLDHVYEEVWRGAELMAWLYCLNLLFHFRE
                     ADGDRIGGRIRHFLDHRGFLTKLHREVSARSGCHGRHRQCPAQIITAEAVKARLVITN
                     HHKLALLAHDPVLSKLFRIFVIDEANHFEDACRSASALEIDSRDIALAVDYIATAAAK
                     LQDRAAGDMGEEIGAILAATEDLHVAIDGFGRALRKIKADCGNIAAAGTCPLPAADAA
                     YLDGHIHTHLRLLREILLMIHDHLAWLKDDDICRLLKIPSRTRERLKNARGEVADQAD
                     RWLEMDNMITRPDRTTAFTILVRHWTIAAAPIDVSDPIRRDFYGDTKGLIYTAATIRH
                     GGSFRDFKKIVGLDRPFPLDEKGEIWREFRFAAIPSPFSGANREVLLHPDAVNGRYEN
                     KEQWLDAVAKVLPGLIQRHHGGTLVLFASYNDLEAVAARIGTEIEALGFPLLVQRRGA
                     PTADLCDEFREIRESVLFGVDSFWYGVDFRGDTLTQVVITRLPLASPADPLQIARRRT
                     MTVGEYQRRYRYDSAIKMNQGMGRLIRSETDYGRIVILDSRWRSQGASGG"
BASE COUNT          604 a          961 c          915 g          648 t
ORIGIN      
        1 cctatccggc cggagcggtc gtgttcaaag acaagcgcat ccttgccgtc cagaccttcc
       61 atgccgccta tgtgcaggac ttgcgcgccc aggccccggt gagcatcggg cactatgttc
      121 tcgagggctc gaagcccggc gccgccgccg cggccgcctg gaccgcccat cgggttttgc
      181 cgttgaacat cgatggatac ggccggctcc tgggccgcac cgtcagaacc acgaacatgc
      241 tctccgcagg catgaccgca gcggcgcccc tggtgacgaa aagcggccgg agataccggt
      301 ttgtggtcct ggccgaaccg gatctgaaca tcctggacta cgtgcttctg gaggtgggga
      361 acccgagcct gaaggccatg aacaagctga atcaggccat ttacgaagca tgttcctata
      421 tctcggggga cctgatgaaa aaggatttta tcgtctccaa gacggctttc tcccccgccg
      481 aatatggaaa caggcccctg gattttatcc ggaaatgcgg ctttgcggat cgggaatggg
      541 aaaagacgcc ctcggtcctg gtgctgagat caaccgtcat gacgccctat ctggccgggg
      601 aggaggattt cggcgcctat tttgagaggc tcagggcgat actgggccgg attctctccc
      661 gaatcgaagc tcagggcgcc ggcgacgggc ccatatccgg cggacgcgga atgcctattt
      721 ggccggcaaa ctcatgacca tgcgcttcag gctgcccttg aagagcacgt ccatgcgctg
      781 atcttccagg atcttctcca cgaagccgat cccgaaggtg ggatgttcga tgacctcgcc
      841 gcgctggtaa cgatcggtca tgcggtaggg tcgtatcggg gtgttcttcc ccagttcgtt
      901 atggcgctgg agccagcgct gggtctccac cgcctcggcc tgccggcttt cttccgcggc
      961 cctcgtcccg gttcggcggg tacggtcgcc gccggtttcg cctttccggt cccggacctt
     1021 ggcgccccgg tatttgtgct ccgccccgca ggtcttgcac ttcacccggg cgatcacccc
     1081 cccgacctca taaaggacga tatgggccag ggtcaggccg cagcgcgtac agagcgcatc
     1141 gacattctta cctaccatgg acataagcac ccctcctgaa gctgatcatg cttcataaca
     1201 tacttttccc cggggcgaac attttttttc cggagatcgc cccggcagcg aaaaaaatcc
     1261 cccgccggac cccgttttcc atcaggagcg tgaaaaacct ctccgccgac ttcccggcaa
     1321 aaccgatggt aaataattca ataatattag ccacttcttt tcctttctct catcccatgt
     1381 gtaggcgacc ttttcacttc gatcgtaatg taaacatctt ttttgggcgt ttcatcctta
     1441 cgtatatctt taaccaccgg aagcaccctg gctgcgccag cgggaatcca gaatgacgat
     1501 gcgcccgtag tccgtttcgc tccggatcag gcggcccata ccctgattca tcttgatggc
     1561 gctgtcatag cgataacgac gttgatattc ccccaccgtc atggtccggc ggcgggcgat
     1621 ttgcagggga tcggcggggc tggcaagggg caggcgcgta atcaccacct gtgtcagggt
     1681 atccccccgg aaatccaccc cataccaaaa gctgtcgacg ccgaagagga cgctttccct
     1741 gatctcccgg aactcgtcgc agagatcggc ggtcggggcg ccccggcgct ggaccaggag
     1801 gggaaacccc agggcctcga tctccgtccc gatcctcgcc gccaccgctt cgaggtcgtt
     1861 atagctggcg aaaagcacca gggtgccgcc gtggtggcgc tggatcagtc ccggcagaac
     1921 cttcgccacg gcatcgagcc actgttcctt gttttcatag cggccgttga ccgcatcggg
     1981 gtgaagcagc acctccctgt tggcgccgga aaacggcgag gggatggcgg caaatcggaa
     2041 ttcccgccat atttctcctt tttcatccag gggaaaggga cgatccaggc caacgatttt
     2101 cttgaagtcc cggaagcttc ctccgtgtcg gattgtcgcg gccgtataga tcagaccttt
     2161 ggtgtccccg tagaaatccc ggcgaatcgg gtcggacaca tcgataggag ctgcagcgat
     2221 ggtccagtgc cggaccagga tcgtgaaggc cgtggtccgg tccggccggg tgatcatgtt
     2281 gtccatctcc agccatcgat cggcctgatc cgccacttct ccccgggcgt ttttcagcct
     2341 ttcccgagtc cggctgggga tcttcaggag gcggcagatg tcatcatcct tgagccaagc
     2401 tagatggtca tggatcatca gcaggatttc ccgcagcagg cgcaggtgcg tatggatatg
     2461 gccgtcgagg taagcggcgt ccgccgcggg gagcgggcaa gttccggccg cggcaatgtt
     2521 gccgcaatcg gccttgatct ttcgcaaggc ccgaccgaag ccgtctatgg ccacatgcaa
     2581 atcctccgtg gccgccagga tagcgcctat ttcttccccc atatcacccg ccgcccggtc
     2641 ctggagtttg gcggcggcgg tggcgatata atcgaccgcc aaggcgatat cccgggaatc
     2701 tatctccagg gcggaagcgc tacggcaggc atcctcgaaa tgattcgcct catcgataac
     2761 aaagatccgg aagagtttgc tcaaaaccgg atcatgggca aggagggcaa gtttgtggtg
     2821 atttgtgatc accaggcggg ctttaacggc ctcggcggtg atgatctggg cggggcactg
     2881 ccggtgcctt ccgtgacagc cggaccgggc ggaaacctcc cgatgcagtt tcgtcaagaa
     2941 accgcggtgg tcgagaaaat ggcgaatccg tcccccgatc cggtcaccgt cggcctcccg
     3001 aaaatgaaag aggagattga ggcagtaaag ccaggccata agttcagccc cccgccagac
     3061 ttcttcatag acgtgatcca gcttttcagc gcagagatag ttcgacttgc ccttgaggag
     3121 ggtgacgg
//
LOCUS       DAOSMF010000204         3124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_33877_length_3124_cov_19.358097, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000204 DAOSMF010000000
VERSION     DAOSMF010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33877_length_3124_cov_19.358097"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            422..1825
                     /locus_tag="PLT63_11070"
     CDS             422..1825
                     /locus_tag="PLT63_11070"
                     /inference="COORDINATES: protein motif:HMM:NF014805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HOD99302.1"
                     /translation="MIRRLQRILIANRGEVVQSITKSLHEMGREAIAVYELPDSETLF
                     IRRADAAIPIGAGPLTDYLNSEKIIRAAKEAGADAIHPGYGFLSESAEFAAACRDNGL
                     VFIGPPPEVMRRLGDKVMAKEMMRQERIVTIPGTEVLASGPAGVAEALAFAEAVGYPI
                     LLKAVEGGGGRGIREVKDAAHLEQQLHHARREALLAFNDDAVYAEKGIVAPRHVEIQI
                     LADAYGKVIHLGSRDCSIQRRHQKLLETAPAAAVPGDIMDAMCRTAVRVARHAGYVNA
                     GTVEFLYDEQQGEFWFMEVNPRLQVEHSVTEMVTGIDIIAKQIEIAEGRPLALEQEDV
                     AAEGCAIHVRVNAEDPGNHFWPEGGKTVVGFRPPAGPGIRLDGVIREGYTVPTVYDSL
                     LVKLTVWGSSWTEAVDRLKEALEKFVIVGLKTTIPFYVAICNEPDFRAGRFDTSYLDL
                     HGDLFDPARHIAANFHP"
     gene            complement(2006..2275)
                     /locus_tag="PLT63_11075"
     CDS             complement(2006..2275)
                     /locus_tag="PLT63_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99303.1"
                     /translation="MASSFFFIGGNLFLMPFYLKYSLELRVDSAGLLFSLYPAAGFIV
                     KGAITSLDAAKKLIDIGGIVVRVVDRAWLEGPNHVKIPLTLFAPG"
     gene            complement(2362..>3124)
                     /locus_tag="PLT63_11080"
     CDS             complement(2362..>3124)
                     /locus_tag="PLT63_11080"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99304.1"
                     /translation="SQDPSPQKEIDKQGLQELLGAYAAAAGRARKAGFDGVQVHMAHG
                     YLLSQFLSPAFNRRRDGYGGDIHNRSRIHREICGAIRDTVGKDYPLLIKLNAQDFIEN
                     GLTVPEAATAARLMVDAGVDAVELSGGMIISGRLSPSRTKINAPEKEAYFREEARWLR
                     ENVQVPLLLVGGNRSYEVAAGLLAEGAADYISLSRPLIREADLIRRWEEGDRRPAACV
                     SDNLCFAPAFEGRGIYCVTAERKAARSGKIVREEP"
BASE COUNT          579 a          988 c          972 g          585 t
ORIGIN      
        1 cccattatct cagcgacgtc ctggccgggg cctacgtggc cgtcatgacg gtgctggtgc
       61 tggcacgcct gcggtatttc cgcctggggc gccccaaata aggaatatga gttggaccgt
      121 tgtcctcctg ggtttgacgg tccgcttcct ctgacggcac gacgactgga tatgcctttt
      181 cctcttcgtt ttcaccaccg tcgccgcctc gggcctcatt acggatatca tcaaacggct
      241 cgcgggcagg tggtttccca ttgacaggcg cagcggcccc ggtatataaa cttgtcgcga
      301 aaaccggatg ttatctcctt ataaaccgcg atgggtgacc ggcgtcatcg cttctttacc
      361 gtccaagaaa ttattttctt cctccccctt acaaggggga gctcaggagg gggatgggcc
      421 aatgatacga aggctgcagc gaatcttgat cgccaatcgc ggcgaggtgg tccagtcgat
      481 caccaaatcc ctgcatgaaa tgggacggga ggcaatcgcc gtttacgagc tacccgacag
      541 cgaaacgctg ttcatccgcc gcgccgatgc ggcgataccc atcggagcgg gcccgctcac
      601 ggactacctg aacagcgaga agatcatccg ggccgcgaaa gaagcgggtg ccgacgccat
      661 tcatcccggc tacggattcc tctccgaatc cgccgaattc gccgccgcct gccgggacaa
      721 cggcctggtc ttcatcggtc cccctccgga ggtgatgcgg cgtctggggg acaaggtgat
      781 ggccaaggag atgatgaggc aggagcggat cgtcacgatc ccgggcacgg aggtgctggc
      841 gtctggaccg gcgggcgtgg cggaggccct ggcctttgcc gaggccgtgg gctatcccat
      901 cctgctgaag gccgtcgaag gcgggggcgg ccggggcatc cgggaggtca aggacgccgc
      961 gcacctggaa cagcagcttc atcacgcccg gcgcgaagcg ctgctggcct tcaacgacga
     1021 cgcggtctat gcggagaagg gcatcgtggc gccgcgccac gtcgaaatcc agatcctggc
     1081 cgacgcctac ggcaaggtga tccatctggg ctcccgggac tgctccatcc agcggcgcca
     1141 tcaaaagctc ctcgagacgg ccccggctgc cgccgttccc ggcgacataa tggatgccat
     1201 gtgccggacg gccgtccggg tggcgcgcca tgccggctac gtcaacgccg gcaccgtcga
     1261 gttcctctac gacgagcagc agggggagtt ctggttcatg gaggtcaacc cccggctcca
     1321 ggtcgagcac tcggttacgg agatggtgac gggaatcgac atcatcgcca agcagatcga
     1381 gatcgccgag ggccgacccc tcgccctgga gcaggaggac gtggccgccg agggctgcgc
     1441 gatccatgtg cgggtcaacg cggaggatcc ggggaatcat ttctggcccg aagggggcaa
     1501 gacggtggtc ggtttccggc cgcccgccgg tcccggcatc cgcctggacg gcgtcatcag
     1561 ggaaggctac accgtcccca cggtctatga ttcgctcctg gtcaagctga cggtttgggg
     1621 ttcctcttgg acggaagccg tggaccggct taaggaggcc ttggagaagt tcgtcatcgt
     1681 cggcctcaag acgacgattc cgttttatgt ggcgatctgc aatgaacccg atttccgggc
     1741 cggccgcttc gacacctcct acctggacct tcacggcgat ctgttcgacc ctgcccggca
     1801 tatcgccgcg aatttccacc cctgagcgcc gttcggcgcc tttctccggc gccaggcgtc
     1861 ggtcggaagg gaaacacaag gcgccttgca gcttcttcgt acggtccggc caaaaggcag
     1921 ggcgccgggg gcggtcctac tcgacaacca ccttgaaggc cgtgagctcc ccttttccgg
     1981 tccagtcacc cttgcattgc accttctaac caggggcaaa caatgtcagg ggaattttca
     2041 catggtttgg cccttccagc caggcccggt ccacgactct gacaacaatg ccgccgatgt
     2101 cgatcagctt ttttgccgca tccagggagg taatggcgcc cttgacgatg aatcccgcgg
     2161 cgggatagag agagaaaagc aaaccggcgc tatcgaccct aagctccagg gaatatttga
     2221 gataaaaggg cattaaaaac agattgccgc cgatgaaaaa aaaagacgat gccaatggcc
     2281 atgatcccga agacgaagcc gctgttttgc agcagggtcg ggtttaccag cggatcgcgg
     2341 gcccggcgga gggccgcttc gctacggttc ttctctcacg atctttccgc tgcgagccgc
     2401 tttccgctcc gcggtgacgc agtagatccc ccggccctcg aaggcggggg caaagcagag
     2461 gttgtcggag acgcaggcgg cgggacggcg gtccccttcc tcccagcgcc ggatcaggtc
     2521 cgcctcccgg atgaggggac ggctcaggga gatatagtcg gcggctcctt cggccaggag
     2581 gccggcggcg acttcataag accggttgcc ccccacaagg agcagcggta cctggacgtt
     2641 ttcccgcagc caccgggcct cctcccggaa ataggcctct ttttccgggg cattgatttt
     2701 tgtcctgctg ggcgaaagac ggccgctgat gatcatcccg ccgctcaact ccacggcgtc
     2761 cacgcccgca tccaccatga gccgtgccgc ggtggccgcc tccgggacgg tgagaccgtt
     2821 ttcgataaaa tcctgggcgt tgagcttgat cagcagggga taatccttcc ccacggtatc
     2881 ccggatggcc ccgcagatct cccggtgaat ccggctgcgg ttatgaatat cccctccata
     2941 gccgtcccgc cgccggttga aggccggcga taggaactga cttaaaagat agccgtgggc
     3001 catatggacc tggacaccgt cgaagcccgc tttccgggcc cgccccgccg ccgcggcata
     3061 agcgccaagg agttcctgca aaccctgttt atctatttcc ttctgggggg atggatcctg
     3121 gctc
//
LOCUS       DAOSMF010000205         3111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_34056_length_3111_cov_15.248691, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000205 DAOSMF010000000
VERSION     DAOSMF010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34056_length_3111_cov_15.248691"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="PLT63_11085"
     CDS             <1..259
                     /locus_tag="PLT63_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99305.1"
                     /translation="YRPEDREPAAFDFAETEPRREEYPIYLGHEGMKKFVAHFEARLN
                     QKALYVPKGQRLTYRQICLEQVRLWVRSLRGEETYTAFTTR"
     gene            664..939
                     /gene="cas2"
                     /locus_tag="PLT63_11090"
     CDS             664..939
                     /gene="cas2"
                     /locus_tag="PLT63_11090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HOD99306.1"
                     /translation="MFVVVSYDVVADKRRNKIAKTLVGFGSRVQKSVFECELDDRNYL
                     KMKDALEKLIDREEDSVRYYFLCGRCRGHVEVSGLGALREEEELIIV"
     gene            1611..>3111
                     /locus_tag="PLT63_11095"
     CDS             1611..>3111
                     /locus_tag="PLT63_11095"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HOD99307.1"
                     /translation="MIASAFMSLLLAVYSWQRRFIAGGPWFTLLMVSITLWAFFAGLS
                     AMVSDREMNILLYKLAYSGVVSVATLWFIFAVRYSGRDAWLKRSLLPLFWIWPVIALV
                     AAWTNEYHYLTWTSIALVPGTSVPVFNYALGPLVFVNIVYSYVLLLIGMIIYVQATAS
                     TQKLYAMQTWVLFLAVIIPWLCNIAFVAGVFPPGIEPTPLAFVAASFLFAWSFYKYKL
                     FDLLPVAHEDIVAGLSDGVVVVDEYNRIMEVNASAREYFHLPERCVGCPATGLIPCWS
                     EVTTIIDERDDARADVKFEQGGTEARWFNIRMSRIKDRSGRPKGRLFIFRDISERKRG
                     EDDLMKLASLDSLTGVFNRRMGLIILERQLQLAKRLGSPLTICYIDIDGLKEVNDRYG
                     HGEGDNLIVRIAKIISESIREQDSLCRLGGDEFLVIQPTCTTENAEVFRTRLDDSFKR
                     LNRDHPRSYRYAASYGFAEYRPDMTVSVDKLINLADAAMYREKMKSRDSG"
BASE COUNT          672 a          808 c          934 g          697 t
ORIGIN      
        1 ctaccgaccc gaggaccggg aaccggcggc cttcgatttc gccgagacgg agccgcgacg
       61 ggaggaatat cccatctatc tcggccacga ggggatgaag aaattcgtcg cccatttcga
      121 ggcccgcctg aatcagaagg cgctgtacgt cccgaagggg cagcgcctca cctaccggca
      181 gatctgcctg gaacaggtcc gcctctgggt ccggagcctc cggggggaag agacgtacac
      241 ggccttcacc acccgatgat caaatcgggg gtcaggtctt taaaattagc atttttacgg
      301 gtggccgtgg gagctgccga acgcctctct tggtaaagat acggaaaaag aaaacgcagc
      361 cgtccccaca ttgcgtgtag gaaaataagg aatggcgggg ccgggccgcc gctattgacg
      421 ccgcgatggc ggcgtacgga tagaaaggac agaggtgggg gtaggggccg ggccgaggta
      481 gggggcgggg caggaacgtc gcgattgacg ttgcgatagc ggcgtacgga taggaaggac
      541 agtggcgggg gctgggccgg gaggggcccg gggaggggcc gggacgccgc tattgacgtt
      601 gcgatagcag cgtacggaga ggagggactg gggccgtgac cgtggccgcg gcaggaggtg
      661 cggatgtttg tggttgtttc ctatgacgtc gtcgccgaca agcggcggaa caaaatcgcc
      721 aagaccctgg tgggcttcgg gagccgggtc cagaagagtg tcttcgagtg cgagctggac
      781 gaccgcaact atctgaagat gaaggacgcc ctggaaaagc ttatcgaccg ggaggaggac
      841 agcgtccggt actatttcct ctgcgggcgc tgccgggggc acgtggaggt gagcggcctc
      901 ggcgccctcc gggaagagga ggaactgatc atcgtttagt ttcttgcctt ggctcacctt
      961 cctcaggttc cctcttacat atcctgtccc accgcccttt cctatatgaa cgctttgaga
     1021 tggaggtgct ggaccggcta aacagtggtc gctttttgga ccggctgatc tttggcggcg
     1081 acaatggcag gggaagagga agcccaagcc caagaatatg cgggatctga ccgccagctg
     1141 cccacaaaat gggcggcgat gcccacaaaa tgggcggcga tgcgggcaaa agggacggct
     1201 ctgtttcgtt aagtatgctt aatcatttac atatttaatt gtggcacggt ctgtgctgta
     1261 agagaggcaa aacaacgacg gtaaaaaagc gaaaggcgga ggacaactgg aaggccccga
     1321 aagcataaga aaccattctc ccatctcact ggggccaggc ctggccttac cacccaggtc
     1381 cggccccagg cctcttttat gctttagggg tcaatatttt tgccgatgaa aacattgcct
     1441 ttcgggttat gaagacaggg tcgcgaacgc cgtagaagtc aacattgact tcattatcgt
     1501 cataaggaaa gggatcattc gccgccggtt ggcctgcttg atgccgagtt gtaacggcgg
     1561 caagtttaaa acaggctatg gatcttcatt acacattgat cggaccgata atgatcgcaa
     1621 gtgccttcat gtcgctcctg ctggccgtat attcctggca gcgccgcttc attgccgggg
     1681 gcccctggtt taccctcctg atggtttcca tcacgctctg ggctttcttc gccggcctgt
     1741 ccgccatggt ttctgaccgg gagatgaata tcctcctgta taaacttgcc tactcaggtg
     1801 tggtcagcgt ggcgacccta tggttcattt ttgccgtacg atacagtggc cgcgatgcct
     1861 ggctgaagcg atccctcctg cccttgtttt ggatttggcc ggtgattgcc ctcgttgccg
     1921 cctggacaaa cgaataccat tacctgacct ggacttccat tgccctggtt cccggcacgt
     1981 ccgtcccggt attcaattat gccctggggc cgctggtgtt tgttaatatc gtgtattcat
     2041 acgtacttct tttaatagga atgatcatct atgttcaagc cacggcgtcc acgcaaaagc
     2101 tctatgccat gcagacctgg gtacttttcc tggcggtgat aattccctgg ctgtgcaaca
     2161 tcgcgttcgt ggccggcgtt tttcccccgg gaattgaacc gacgcccctg gccttcgtgg
     2221 cggcaagttt ccttttcgca tggagtttct acaagtataa acttttcgac ttgcttcccg
     2281 tagcccatga ggacatcgtt gccggcctga gtgacggtgt cgtggttgtt gatgaataca
     2341 accggattat ggaagtcaat gccagcgcca gggaatattt ccatcttccc gagagatgtg
     2401 tcggatgccc cgccacaggt ctcattcctt gctggtcgga agtgacaaca attatcgatg
     2461 aacgggatga cgcacgggcg gatgttaaat tcgaacaggg gggaacggag gcaagatggt
     2521 tcaatattcg tatgtcccgg atcaaggacc ggtccggccg tccgaagggc cgcctgttca
     2581 tatttcgcga tatcagcgaa cgaaaaaggg gggaagatga tctgatgaaa ctggcctctc
     2641 tggacagcct taccggagta tttaaccggc gcatgggact catcatcctg gagcggcagc
     2701 ttcaattagc caaaaggctc ggttctccgc tgacaatttg ttatatagac atcgatgggc
     2761 tcaaggaagt caatgaccgg tatggtcatg gcgaggggga taacttaatc gtgagaattg
     2821 ccaagatcat cagtgagtct atccgtgaac aggattctct gtgccgcctc ggcggcgatg
     2881 aattcctcgt tattcagccc acctgtacca ccgagaatgc cgaggtcttc agaacccgcc
     2941 tggatgactc tttcaagagg ctcaatcggg accatccccg gtcctatcgc tacgccgcgt
     3001 cttacgggtt tgccgaatac cggccggata tgaccgtttc ggttgataag ctgattaacc
     3061 tggccgatgc ggcaatgtac cgggaaaaga tgaaatcacg tgactccgga c
//
LOCUS       DAOSMF010000206         3074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_34598_length_3074_cov_24.958927, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000206 DAOSMF010000000
VERSION     DAOSMF010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34598_length_3074_cov_24.958927"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(270..584)
                     /locus_tag="PLT63_11100"
     CDS             complement(270..584)
                     /locus_tag="PLT63_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99308.1"
                     /translation="MERKGTMHAITGGLILITLGVLIILGKTGIWSFNASWPVLLIAI
                     AVGTLIQNVKDLGGWIILVVGVIFLLTEIFGVQVYALGKYLLPVLIIVVGANILMKQR
                     KR"
     gene            complement(643..1392)
                     /locus_tag="PLT63_11105"
     CDS             complement(643..1392)
                     /locus_tag="PLT63_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HOD99309.1"
                     /translation="MTYTITIIVFLIIMFLASAIRIMNEYERAVIFRLGRIIDMKGPG
                     LIILIPVIDKMVKVDMRIITMDVPAQDVITRDNVSIKVNAVVYFRVMDANSAVTEVEN
                     FLYATSQLAQTTLRSVCGQVELDEILSEREKINLHLQEILDRSTDPWGIKVTLVEVKH
                     IDLPEEMKRAMAKQAEAERERRAKVIAAEGEFQAAEKLMEAASVMAREPITLQLRYLQ
                     TLNQIASENNSTTVFPIPIDMFSHFLGKGNK"
     gene            complement(1410..2729)
                     /locus_tag="PLT63_11110"
     CDS             complement(1410..2729)
                     /locus_tag="PLT63_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HOD99310.1"
                     /translation="MFQKRYFRAGLTAALLLLLPLLVSVTNVPAAERTPTFHQIAVSG
                     PITPPVAEFILQHIASAGKEGADGLIILLDTPGGLDLSMRDIAKGILNAPVPVLVYVY
                     PSGARAASAGVIITVAAHVAAMAPGTNIGAAHPVGLGGGTGDKTMMEKVANDAAAYVR
                     SIAKIRGKNEDWVEKAVRKSESITAEEALKNNVIDYVATDIRHLLAQAEGRQVIVGTE
                     KRVLKTAGATLTEKKMGHRQRILTTLSDPNIAYILFLLGLAGLYFEFSHPGAILPGVV
                     GGISLILAFFAMQTLPVNYAAVLLVLFGIVLFIAEIKVVSHGILTVGGIISLAVGSLM
                     LFDSPDPALRVSLEVMIPTLTVISLFFAGVIYLVVKAQVRKKLTGKEGMIGAEGKTVT
                     DIDGEGKVMVKGEYWAAYSDEPLKQGMKVKVVKIEGLRIKVTATTER"
     gene            complement(2731..>3074)
                     /locus_tag="PLT63_11115"
     CDS             complement(2731..>3074)
                     /locus_tag="PLT63_11115"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOD99311.1"
                     /translation="EVYICNILKCRPPGNRNPAPAEIAACEPFLVRQLEAIRPRAICA
                     LGTIAAQTLLKTGAPISILRGRWHSYHDIPLMPTYHPAYLLRNPAQKRQVWEDVQQIM
                     RLLDAGTGKGG"
BASE COUNT          615 a          919 c          869 g          671 t
ORIGIN      
        1 tcccccttca gggcgatctc ggcgtaagcg ccgacgccgt tgcactcaca gtaacaggtc
       61 agataaggcc agacgatccg aacccggagg cgataacaat cctcgcaaaa tcccatcatc
      121 gccgccagat ccgggcaaac caaagacaat ttctcatccc cggccggcat cttctcctcc
      181 ggtaatcgcg accgctcggg tgttaattac ttttgttctt ttgtcagacg cggcgcttat
      241 tgttctcaat gttttatcag ccggggcgct catcgttttc gctgcttcat caggatattg
      301 gcccccacca caatgatcag cacggggagc agatacttgc cgagggcgta aacctggacg
      361 ccaaatatct ccgtaaggag aaagatgaca cctacgacca gtatgatcca accgcccaga
      421 tccttaacgt tctggatcaa ggtgcccacg gcgatggcga tgagcagtac cggccagctc
      481 gcattgaagg accagatgcc cgtcttgccc aggatgatga ggacgcccag ggtaatcaga
      541 atcaggccgc cggttatggc gtgcattgtc ccttttcttt ccattgacca ttcccccatg
      601 cttccggtca gaaaccttcc ggttgtcggt ttctttaaag gatcatttat tgcccttccc
      661 caggaagtgg ctgaacatat cgatggggat ggggaacacc gtggtggagt tgttctccga
      721 ggcaatctgg ttcagggttt gtagatagcg caactgcagt gtaatgggct cccgcgccat
      781 aacggatgcc gcctccatga gcttttccgc cgcctgaaat tctccttccg ccgcaatgac
      841 tttggcccgt cgttctcgtt ccgcctcggc ctgtttggcc atggcccgct tcatctcttc
      901 cggcagatcg atgtgcttca cctccaccag ggtcaccttg atgccccagg gatcggtgct
      961 ccggtccagg atctcctgaa gatggaggtt gatcttctcc cgttccgaca ggatctcgtc
     1021 caactccacc tggccgcaga cgctccggag ggtggtctgg gcaagttggg acgtggcata
     1081 gaggaagttc tccacctccg ttaccgccga attggcgtcc ataacccgga aataaaccac
     1141 ggcgttgacc ttgatggaaa cgttgtcccg ggtgatgaca tcctgggcag gaacgtccat
     1201 ggtgatgatc cgcatatcca ccttgaccat cttgtcgatc accgggatga ggatgatcag
     1261 gccggggcct ttcatatcga tgatccttcc cagccggaag atgacggccc gttcgtactc
     1321 gttcatgatc cggatggccg aggccaaaaa catgatgatg agaaaaacga tgatggtaat
     1381 cgtgtatgtc atgctgcttc cctccttatc tatctttccg tggttgccgt cacctttatc
     1441 cgcaagccct cgatcttgac cactttgacc ttcatcccct gcttaagtgg ttcgtcgctg
     1501 taagccgccc agtattcccc tttgaccatt accttaccct cgccatcgat gtcggttacc
     1561 gtcttgcctt ccgcgccgat catcccctcc ttgcctgtaa gttttttccg aacctgggcc
     1621 ttgaccacca gatagatcac cccggcaaaa aagagggaaa tcacggtcaa agtagggatc
     1681 atcacctcca gggacacccg cagggccgga tccggggagt cgaaaagcat cagggaaccc
     1741 acggcgaggg agatgatgcc cccgacagtc aggatcccat gacttactac cttgatctcg
     1801 gctatgaaca gcacgatccc gaataggacg agcaggaccg cggcataatt caccggcagg
     1861 gtctgcatgg cgaaaaacgc gaggatcagg gatattcctc ccaccacgcc ggggaggatt
     1921 gccccggggt gggagaattc gaagtagagc cccgccagcc ccagcaggaa aaggatatag
     1981 gcgatattgg gatcgctcag ggtggtaagg atccgctgcc ggtgccccat ctttttctcc
     2041 gtcagggtcg ctcccgccgt tttcaagact cttttttcgg tgcctacgat cacctgcctt
     2101 ccctccgcct gggcgaggag atggcggata tcagtggcga cataatcgat gacgttgttt
     2161 ttcagcgcct cttccgccgt gatggactca ctcttgcgta ccgccttttc cacccagtcc
     2221 tcgtttttcc cccggatctt ggcgatgctg cggacatagg cggcggcgtc attggccacc
     2281 ttttccatca tggtcttgtc gcccgttccc ccgcccagac ccacggggtg ggcggcgccg
     2341 atgttcgttc ccggggccat ggccgccacg tgggcagcca cggtgatgat aacaccggcg
     2401 gaagcggcgc gggcgccgga gggatataca tagaccagaa ccggcaccgg ggcgttgagg
     2461 attcccttgg caatatcccg catggaaaga tcgagccccc ccggggtatc gaggagaatg
     2521 atcaggccgt ccgccccttc cttgccggcg gaggcgatat gctggaggat gaattccgcc
     2581 accgggggcg tgatgggacc gctcacggca atctgatgaa aggtgggggt ccgctccgcg
     2641 gccgggacat tcgtaacgga aacaagcagg ggcaggagca ggagcagcgc cgccgtcaaa
     2701 ccggcgcgga aatagcgttt ctgaaacatt tcaacctcct ttgccggtcc cggcatcgag
     2761 gagccgcata atctgctgga cgtcttccca cacctggcgc ttctgggcgg gattccgcaa
     2821 cagataggcc gggtgatagg tcggcatcag gggaatgtcg tgatagctat gccagcgtcc
     2881 ccggagaatg gagatgggtg ctccggtctt caggagcgtc tgggcggcta tagtccccag
     2941 ggcgcagatc gcccggggcc tgatggcctc cagttgccgg accagaaacg gctcgcaggc
     3001 ggcgatctcc gcgggggcgg gattgcggtt tcccggcggc cggcatttga ggatattgca
     3061 gatgtaaacc tccg
//
LOCUS       DAOSMF010000207         3072 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_34627_length_3072_cov_30.673185, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000207 DAOSMF010000000
VERSION     DAOSMF010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34627_length_3072_cov_30.673185"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..370)
                     /locus_tag="PLT63_11120"
     CDS             complement(50..370)
                     /locus_tag="PLT63_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99312.1"
                     /translation="MEHFLTFYDVVYHNYTSTVESDNKQHPRAIFFISFKPLKISFND
                     MPLRAGKLLIPVGVVFVIAVYNQLDTFLFVGDGIVARIFHYRKSNTAGKKEIKIKKSG
                     GHRK"
     gene            545..727
                     /locus_tag="PLT63_11125"
     CDS             545..727
                     /locus_tag="PLT63_11125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99313.1"
                     /translation="MADTKPQLPDSFWKCSNCGNTIQEERPPQACPVCRQQCQFINVT
                     CYTPDCGFTGLDQRLK"
     gene            766..1344
                     /locus_tag="PLT63_11130"
     CDS             766..1344
                     /locus_tag="PLT63_11130"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOD99314.1"
                     /translation="MSKSIKGSKTEKNLLAAFAGESQARMRYTYFASAARKEGFEQIA
                     NIFMETAENEKEHAKIFFKHLEGGEVEITAAYPAGVIADTKTNLEAAAAGEQMEWTTL
                     YSNFAAIAKEEGYPEVARSFEQIAKVEKFHENRYRAFIANIANGEVFKKKQPVKWHCI
                     NCGYVHEGMEAPKNCPACLHPQSYYEVLAENV"
     gene            1357..1776
                     /locus_tag="PLT63_11135"
     CDS             1357..1776
                     /locus_tag="PLT63_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOD99315.1"
                     /translation="MKSYRKELWFNVPARRGFINITPQVRQCLRESGILEGMILVNAM
                     HITASVFINDDEAGLHADYDDWLEGLAPHAPTSRYRHNRTGEDNGDAHLKRQIMGREV
                     VVAVTAGELDFGPWEQIFYGEFDGRRPKRVLVKIIGE"
     gene            1803..2735
                     /locus_tag="PLT63_11140"
     CDS             1803..2735
                     /locus_tag="PLT63_11140"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOD99316.1"
                     /translation="MIYLKLFLTAVLWGGTFVAGRIAAQDMGPASGSFLRFAVASLLL
                     IAMIWQAQGRLPIPARRQFIPLFLLGLSGVAAYNIFFFLGLKLIPASRASLIVASNPV
                     FIALGAALLFKERLTAARGAGIALCLTGAVIVISRGDLPGLLGAGIGTGETFILGCIV
                     SWVSYSLIGRGVLKEIPPLVAVAYACIIGTALLALPALCEGMLPALPGYSLTSWSAVL
                     FLGLLGSAVAFTWYYEGIKALGPTRAAVFINFVPVSGVFLGWLILDEALNLSLLLGAA
                     LVITGAWLTNRTPAREGKPPARPTRGPHSNLTTS"
BASE COUNT          651 a          933 c          856 g          632 t
ORIGIN      
        1 gtgcagagca ccactaccaa agccgctacc atcgttccta atgtcttttt cattttctgt
       61 gtcctcctga tttttttatt tttatttctt ttttccccgc agtgtttgac tttctataat
      121 gaaaaatgcg tgccacaatt ccatccccaa caaataaaaa cgtatctaat tgattataaa
      181 cggctattac aaataccacc ccaacgggaa tgaggagttt gccggcccgg aggggcatat
      241 cgttaaaaga tattttcaag ggcttgaacg aaatgaagaa gatcgcccgg ggatgttgct
      301 tattatctga ttctacggta gaagtataat tatggtagac gacatcgtaa aaggtcaaaa
      361 aatgttccat ttgggacggc atcgtaaaaa gatccgcagg cgaggcgcgc gaatcctgag
      421 gaatgaggcg tacttgtacg tacgtcgcag tgacgaggga tgaagcgcaa cgaagcatcc
      481 ggacttttta cgatgccgtc atggtaaaca gacgagagaa gaaataagga aaggaggtac
      541 cgtcatggcg gataccaaac cccagcttcc cgattcattc tggaagtgca gcaactgcgg
      601 gaatacgatc caggaggaaa gaccgccgca ggcgtgcccg gtctgccggc agcagtgcca
      661 gttcatcaac gttacctgct acaccccgga ttgcggattt acgggacttg accagcgtct
      721 gaagtgacat tatattacgg aaccattaaa gacaggaggt aagcaatgag caaatcgatc
      781 aagggaagca agacggaaaa gaacctgctg gcggccttcg ccggcgaatc ccaggcccgg
      841 atgcgctaca cctatttcgc cagcgccgcc cgcaaggagg gctttgaaca gatcgccaac
      901 atcttcatgg agacggcgga aaacgaaaag gaacacgcca agatattctt caagcacctg
      961 gaggggggag aggtggagat taccgccgcc tatcccgccg gggtgatcgc cgatacgaag
     1021 acgaatctcg aagcggcggc ggcgggggag cagatggagt ggacaacgct ctattccaat
     1081 tttgccgcca tcgccaagga agagggttat cccgaggtcg cccgttcctt cgagcagatc
     1141 gccaaggtgg aaaaattcca tgagaaccgc taccgcgcct tcatcgccaa tatcgccaac
     1201 ggcgaggtct tcaagaaaaa gcagcccgta aaatggcact gcatcaactg cggctatgtc
     1261 cacgaaggga tggaggcccc gaagaattgt ccggcctgtc tccatcccca atcctactac
     1321 gaagtcctgg cggagaacgt ctaaaaggcc gccgggatga agagctaccg caaggaactc
     1381 tggttcaacg tgcccgcgag acgcggtttt atcaatatca ccccccaggt ccggcaatgc
     1441 ctccgggaat ccggaatcct ggaggggatg atattggtga acgccatgca tatcaccgcc
     1501 tcggtgttca tcaacgacga cgaggcgggc ctccatgccg actatgatga ctggctggag
     1561 ggcctggccc cccacgcccc cacctcccgc taccgtcaca accggaccgg cgaggataac
     1621 ggcgacgccc acctcaaacg ccagatcatg ggccgggagg tcgtcgtggc cgtcacggcc
     1681 ggggagttgg atttcggtcc ctgggagcag atcttctacg gcgagttcga cggccggcgg
     1741 ccgaagcggg tcctcgtcaa gatcatcggc gaatagcggc ggccgctcac ggtgacaacc
     1801 tcttgattta tctcaaatta tttctaaccg ccgtcctctg ggggggcacc ttcgtcgccg
     1861 ggcggatcgc cgcccaggac atggggcccg catccgggtc ctttctccgg tttgccgtcg
     1921 cctccctttt gctcatcgcc atgatctggc aggcccaggg acgtctgccc atccccgccc
     1981 ggcggcagtt tatccccctg ttcctgctgg ggctctccgg cgtggcggcc tacaacatct
     2041 tctttttcct cggcttgaag ctcatccccg ccagccgcgc ctcgctcatc gtcgcctcca
     2101 accccgtttt catcgccctt ggagcagcgc tcctgttcaa agagcggctg accgccgccc
     2161 ggggggcggg catcgccctg tgtctcaccg gcgccgtcat cgtcatctcc cggggagatc
     2221 tgcccggact cctgggcgcc ggcatcggta cgggcgagac attcatcctg ggctgcatcg
     2281 tcagttgggt cagctattcc ctcatcggcc ggggtgtcct gaaggagatc ccgcccctgg
     2341 tggccgtggc gtacgcctgt atcatcggca cggcgctttt ggctctcccc gccctctgcg
     2401 aggggatgct ccccgccctg cccgggtatt ccctcacctc ctggtccgcg gtcctctttc
     2461 tggggctcct gggctccgcc gtggccttca cctggtacta cgaggggatc aaggccctgg
     2521 ggccgacccg ggctgccgtc ttcatcaact tcgtccccgt aagcggcgtg ttcctgggct
     2581 ggctgatcct cgacgaggcc ctcaaccttt ccctcctttt gggcgccgcc ctggtcatca
     2641 cgggcgcctg gctcaccaac cggaccccgg cccgggaggg gaagcccccc gcgcggccca
     2701 cccgggggcc gcactcaaat ttgacgacat cgtaaaaggt caaaaatgtt ccatttggga
     2761 cggcatcgta aaaagatccg caggcgaggc gcgcgaatcc tgaggaatga ggcgtactgg
     2821 gacgtacgtc gcagtgacga gggatgaagc gctttcgaag catccggact tttttacgat
     2881 gtcgtcaaat ttgattgaca cacttctccc cttgccctat atcttgaccg gataatataa
     2941 tatatccctg ttctccagac atcagttcca gtggttcatt agcatccagc ccggaacgat
     3001 ccaccaaatg ccgcgccccg cccgcgggcg cgcaactctc ggcgttagca ggcgcgcctc
     3061 tctgcctgcg gg
//
LOCUS       DAOSMF010000208         3012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_35540_length_3012_cov_21.142374, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000208 DAOSMF010000000
VERSION     DAOSMF010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35540_length_3012_cov_21.142374"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            465..1391
                     /locus_tag="PLT63_11145"
     CDS             465..1391
                     /locus_tag="PLT63_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99317.1"
                     /translation="MNDRNKQLANLLNTEDHRAVFEEIMITVSLIFPRFDFTVFTRVR
                     EDIVLLFQGRYPRFRRSNLKFHDLTHTTSVTLAVARLMHGAVETGHSFTEKDVNMGLI
                     SALMHDTGYIQSEDDTEGTGAKYTMIHVDRSIAFLKEYYRNDPLFKEDLPAFQDILHC
                     TGLNTKITEIRFSSKTVALLGKILGTADLLGQMAERYYLERLLDLYNEFVEGGIRTFK
                     SRLDLLEKTRGFYEMTRRRFTEELGDVYRFSRHHFRKRWGIDEDLYIQAIENNITYLD
                     RVLTHHRRDYRSHLRRRYGSVSSGKPTHQAPE"
     gene            1398..1835
                     /locus_tag="PLT63_11150"
     CDS             1398..1835
                     /locus_tag="PLT63_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99318.1"
                     /translation="MIYHTFLFDENIWIAGGVYIDDHGRYHAVKGESRIIHADVLWTN
                     ESRMILEDNPAVEFRNRYDILPMDRKQEWTSWSSENPALGKLTGRFILVDDSILSSFA
                     SKDDSFQGMEYLKMIDDDLYWNRGCLFLGERKISSWAVELRRK"
     gene            complement(1912..>3012)
                     /locus_tag="PLT63_11155"
     CDS             complement(1912..>3012)
                     /locus_tag="PLT63_11155"
                     /inference="COORDINATES: protein motif:HMM:NF024774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase C-terminal domain-containing
                     protein"
                     /protein_id="HOD99319.1"
                     /translation="LNAYVGAGIRSDHECTQLAEAREKLRLGMQIMIREGTQAKNLAA
                     LVPLVNAATLRQCCLVTDDLHPHDILRHGHLDVVMSLAIEMGLPPLWAVAMVTLNTAR
                     YFSLPDRGAVAPGYRADLALLSSLDPVRVSHVFKDGRHAVDKGELAIDIPTAGLPAGV
                     SSMKVKPYDVTAFELSALGKHALVIDLIPDQILTGKAVLPVRVQNGLVVQDPRRDILK
                     LAVIERHRGTGNIAVALVRGFSLREGALATSVAHDSHNIICVGCTDADMANAVRAVEE
                     MKGGMAIACDGRIVARLPLPIGGLMSDRPLAETAGLWEDIRYQAQQLGCRLSEPFMHL
                     SFLALPVIPELKLTDRGLVDVDKFAFVPLFVD"
BASE COUNT          716 a          849 c          808 g          639 t
ORIGIN      
        1 atcgcctgct cttccaagcc aagcagatcc tcctggtccg ggcggcgagt tacgccttcg
       61 aagcaaccta tgaatacctg gccctcttcc ccctggccgc tctcgtctgc gtagtcctgg
      121 taaaaggtaa ttccaatcac cgaggcatca aaccgccttt acactgagcg ccggaaataa
      181 gagatcaccc ttcgacttac cattccccgg caatctccgt ctgaccggcg gtatgaggat
      241 tcaattttaa ttcatattat gattaagaag ttaatataat atttgcatta attttaaaat
      301 aatgtctatc aatcatagaa ttcttcttgc aatctcatta tcaaataggt taggcatcta
      361 taattattaa tccacaacga tcaaagccgt ataatcagat attttccgcc caatccggag
      421 gacaggccgg tgcggggaga tgaaaatgct gctggaaaac gccgatgaac gatcgtaaca
      481 aacagttggc caatctcctg aataccgagg accaccgagc cgtttttgaa gaaatcatga
      541 ttacggtatc gttgatcttt ccccggtttg acttcaccgt ctttacgcgc gtccgggagg
      601 atatcgtcct gctcttccaa ggcaggtatc ctcgtttccg tcgatccaac ctgaagttcc
      661 acgacctgac ccacaccaca tcggtcaccc tcgccgtggc ccgactcatg catggggccg
      721 tcgaaaccgg acattccttc accgagaaag atgtaaatat gggactcatc agcgcgctca
      781 tgcatgatac cggctacatc cagagcgagg acgacaccga gggaacgggg gcgaaatata
      841 ccatgatcca tgtggacaga agcatcgcct tcctgaagga atattaccgc aacgatccgc
      901 ttttcaagga agatcttccc gcgtttcagg atatcctcca ttgtaccgga ctcaacacca
      961 agattacgga aatacgtttc agcagcaaaa cggtggcctt gctcgggaaa atactcggca
     1021 cggcggatct tttgggccag atggccgagc gctattacct ggagaggctc ctcgatctgt
     1081 acaacgagtt cgtggagggc ggcatcagga cctttaagag ccggttggat ctattggaaa
     1141 agacccgggg gttctacgag atgacccggc gccgttttac cgaggagctg ggtgatgtct
     1201 accggttttc ccgccatcat tttcggaagc gctggggcat cgacgaggac ctctatatac
     1261 aagccatcga aaacaacatc acctatctag atcgggtcct cactcaccac cgccgtgact
     1321 accgttcgca cctgaggagg agatatggct cggtgtcgtc agggaaacca acgcaccagg
     1381 cgccggagtg agggacaatg atatatcata ccttcctttt tgatgaaaat atctggatcg
     1441 ccggcggggt ttacatcgat gaccacggtc gctaccatgc ggtgaaggga gaaagccgga
     1501 tcatccatgc ggacgtttta tggaccaacg aatcccgtat gatcctggag gacaatcccg
     1561 ccgtggaatt ccggaatcgt tacgatatcc tcccaatgga caggaaacag gagtggacaa
     1621 gttggtcttc ggaaaatccc gcgctgggaa agctgacggg aaggtttatc ttggtcgacg
     1681 attccattct atcttcattc gcttcgaaag acgactcgtt tcagggcatg gaatacctga
     1741 aaatgattga cgacgatcta tactggaatc ggggatgtct cttcctgggc gagagaaaga
     1801 tatcttcctg ggcggtggaa ctacggagaa aatagcgacc ggggcatcct tttcttcatc
     1861 accgcctgtt acccgcgcgc ggccgcgtcg aaatggcgtt gggctcccgt ctcagtccac
     1921 gaaaagcggc acaaaagcga atttatccac atccaccagt cctcgatccg tcaacttcag
     1981 ttcgggaata acggggaggg caaggaaaga caggtgcatg aacggttcgc taagccggca
     2041 tcccaactgc tgtgcctggt agcgaatatc ctcccagagg ccggccgtct ccgccagcgg
     2101 ccggtccgac atcaagccgc caatgggcaa cggcagccgg gcaacgattc gcccgtcaca
     2161 ggcaatggcc atccctccct tcatctcttc caccgcccgg accgcgtttg ccatgtcggc
     2221 gtcggtacag cccacacaaa tgatattatg ggaatcgtgg gccacggatg tggccagggc
     2281 cccctctcgg agggaaaaac cccggacgag cgccacagcg atgttaccgg tgccgcggtg
     2341 ccgttctata acggcaagct tcaaaatgtc ccgccgcggg tcctgaacga ccagtccgtt
     2401 ttggaccctc acgggaagta ccgcctttcc cgtcaagatc tggtctggga tcagatcgat
     2461 caccagggcg tgcttcccca gcgccgagag ctcgaaagcc gttacatcgt atggtttgac
     2521 cttcatggag gatacaccag ctggcaaacc ggcggtggga atgtcgatgg ccagttctcc
     2581 tttatctacc gcatgccggc catccttgaa gacgtgggac acccgcacgg ggtccaggga
     2641 ggaaagcagc gccagatcgg cccgataacc cggggctacg gcgccacggt ccggaaggga
     2701 aaaataccgg gcggtattga gggtgaccat ggcgacggcc cagagaggcg gcaatcccat
     2761 ctcgatcgcc agactcatga ccacgtccag atggccgtga cgaaggatgt catggggatg
     2821 gagatcgtcg gtcaccaggc aacactgccg gagcgtagcg gcgttgacca gcggcacgag
     2881 cgcggcgagg ttcttggcct gggttccctc ccggatcatg atctgcatcc ccaggcggag
     2941 cttttcccgg gcctccgcca attgcgtgca ctcgtgatcg gagcggattc cggcaccgac
     3001 ataggcgttg ag
//
LOCUS       DAOSMF010000209         2896 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_37409_length_2896_cov_16.403731, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000209 DAOSMF010000000
VERSION     DAOSMF010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37409_length_2896_cov_16.403731"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..550)
                     /locus_tag="PLT63_11160"
     CDS             complement(113..550)
                     /locus_tag="PLT63_11160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOD99320.1"
                     /translation="MQQKTIFLPASQRQVRNLLQSIVSHMLDALLILDWNGVILFANQ
                     AAAELVELDTTEAGIGRNAVEFIHPDFLETVFVDLINVQAGGGDIFNVFSLDGEQLAL
                     YMLNVSGHGVPSALVTVSVSQFLQPHRSGLCVPHGFLPFSVDP"
     gene            834..>2896
                     /locus_tag="PLT63_11165"
     CDS             834..>2896
                     /locus_tag="PLT63_11165"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOD99321.1"
                     /translation="MMVKNMPSYCQPFLSLFILLCLALPPAAAAAKHPFSITDLLAME
                     RIADPQASPDARRVAYTVQQTDLAANRGRLSIHIADLRQGTFSRLPAAGEADDANPRW
                     SPDGRSLYFLSTRSGAQQVWKINLRDQNIRQVTSLPLNVRLFEVSPDGRFLILAMAVI
                     PGKTAEETREIIAARAVRPESGLTYQRLMVRSWDTWSDGTRNHLFYYPLPGGPVRDLM
                     TGMDADCPSRPAGGAEEFSLAPDSRTLVFTAKDEGRREAWSTNSDLFVVSLPPAAQPA
                     PPRRITTNPATDTQPRFSPDGRTLAYLAMSRPGHEADRYRIVVRNLADGRERSLDLRA
                     EAAAGADRSPDYLAWSPDGKTIYCTAEHLGQHVLFAVDVATGKSRLLMKTGNTISPQP
                     LASRGLLLAWNSLIQPTELYLLGTAGEESRRITKTNDHRLENIRFGKMESFTFTGAKG
                     DTVYGRIVYPAEFDPARKYPVAFIIHGGPETSNLNEFHYRWHPQIYAGAGYAVIQIEF
                     HGSTGYGQDFTDAIRGDWGGAPYEDLRKGLAAALGYYPFLDADRMAALGPSYGGYMIN
                     WIGGQEHRFRCLVSHAGIFDKRMAHYEMDTQWFPEWEFGDPGRHNPLDNVRNWHTPVL
                     IIAGRNDYRVSYTQSLALFTALQRQGVPSELLIFPDEGHWILKPQNSRQWHEAVLDWL
                     HRWLQG"
BASE COUNT          619 a          920 c          793 g          564 t
ORIGIN      
        1 gacccgcaag gattagagta ccgatgcgga gcacgggggc ggattacccc gtattagtgg
       61 tgaagtctct gtaatggaga tggagcgaag ggggtgacat gtccaaagct agtcatggat
      121 caaccgaaaa cgggaggaat ccgtgaggaa cgcaaagtcc actacggtgg ggctgcaaaa
      181 actgagatac ggagaccgtg accagggccg acgggacgcc atgcccgctg acattgagca
      241 tataaagggc aagctgctcc ccgtcaaggg aaaagacatt gaagatatcg ccgccgccgg
      301 cctgcacatt gataagatcg acaaataccg tttcgagaaa atcagggtga atgaattcga
      361 cggcgttgcg gccgatgccg gcctccgtgg tatccagctc cacaagttcg gccgcggcct
      421 gattggcaaa caggataacc ccgttccagt cgaggatcag cagggcatcc agcatatgag
      481 acacaatgct ttggaggaga ttccgtacct gccgctggct ggccggcaaa aaaatggttt
      541 tctgctgcac ccggtttatt atccgtcata ccttcatcct tgacaagatc atcaaaatcg
      601 gaaacccata cacagatgga aaccctcctt cttcatccgc ggcaggccac atcaatccgt
      661 gccgccgtgt ggcggccggg ttcggacggc ggcggaataa taccttggaa acgtcttgaa
      721 gatattgaac ttccatgaaa aagtcaatta aatctccacg cctgattatt gtttaattgc
      781 tccggccatg ctatattaca aaagagaaaa tgacaatttc cggaaaacgt ccgatgatgg
      841 taaaaaatat gccaagctat tgccaacctt ttcttagctt gtttattttg ctctgcctgg
      901 ccctcccgcc agcggcagcc gcagccaagc accctttctc cataaccgat ctgcttgcca
      961 tggaacggat cgccgatccc caggcttctc ccgacgcacg ccgggtggcc tataccgtcc
     1021 agcagaccga tctcgccgcc aatcggggcc gcttgtccat tcacatcgcc gacctgcggc
     1081 aggggacttt ttcccgcctt cccgcggccg gggaggcgga cgatgccaat ccccgctggt
     1141 ctcccgacgg gcgcagcctc tatttcctgt ccacccgatc cggcgcccag caggtctgga
     1201 agataaacct gcgagaccag aatataagac aggtgactag cctgcctctg aacgtaaggc
     1261 tttttgaagt atctcccgat ggccggttcc tgatcctggc gatggccgta atccccggca
     1321 agacggctga ggaaaccagg gaaatcatcg ccgcccgggc cgtccgtccg gaatcggggc
     1381 tcacatacca gcgcctcatg gtgcggagtt gggacacctg gagcgacggc acccgcaacc
     1441 acctgtttta ttatccgctg cccggcggtc ccgtccgcga tctcatgacc ggcatggatg
     1501 ctgactgtcc ctcccggccg gcgggcggcg ctgaagaatt ttccctggcc cccgacagcc
     1561 ggaccctggt tttcaccgcc aaagacgaag gccgccggga ggcatggtcc accaacagcg
     1621 atctttttgt cgtctcgctg ccgccggccg cccaaccggc gccgccgcga agaatcacga
     1681 ctaatcccgc caccgacacc cagccccgtt tttcccccga cgggcggacc ctggcctacc
     1741 tggcgatgag tcggccgggt catgaagcgg accgctaccg gatcgtcgtg cgcaaccttg
     1801 ccgacggcag ggaaaggtct cttgatctcc gggccgaagc cgcggccggc gccgaccgct
     1861 cacccgatta tctcgcctgg tctcccgacg gcaagaccat atactgcacc gccgaacatc
     1921 tgggacagca cgtcctgttt gccgtggatg tcgccaccgg caagagccgg ctgctcatga
     1981 aaacgggcaa taccatctcc ccccagcccc tggcctccag aggcctcctg ctggcctgga
     2041 attccctcat ccagcccacg gagctgtacc tgctcgggac ggccggtgaa gaatcccggc
     2101 ggatcaccaa gacaaacgac caccgcttag aaaatatccg tttcggcaaa atggaaagct
     2161 tcacctttac gggggcgaag ggggatacgg tgtacgggag aatagtctat ccggccgaat
     2221 tcgaccccgc ccgcaaatat ccggtcgcct tcatcattca cggcggtccg gagacatcca
     2281 acctgaatga atttcattac cgctggcatc cccagatcta cgccggcgcc ggttatgccg
     2341 tcattcagat cgaatttcac ggctccaccg gctatggtca ggatttcacg gatgccatcc
     2401 ggggcgactg gggaggtgcg ccctatgagg atctccggaa gggcctcgct gccgccctgg
     2461 gttattaccc ctttctcgat gccgaccgga tggccgccct gggcccttcc tacgggggct
     2521 acatgatcaa ctggatcggc ggccaggagc atcgtttccg ctgcctggtc agtcacgcgg
     2581 ggattttcga caagcggatg gcccattatg aaatggacac ccaatggttc cccgaatggg
     2641 aattcggcga cccgggccgt cacaaccctc tcgacaacgt ccgcaactgg cacacgccgg
     2701 tgctgatcat tgccggccgc aacgactatc gggtctccta tacccagagt ctggccctct
     2761 ttaccgccct tcagcgccag ggagttcctt ccgaactcct catttttccc gacgaggggc
     2821 actggatcct caaaccgcag aattcccgcc agtggcacga agccgttctc gactggctgc
     2881 accgttggct tcaggg
//
LOCUS       DAOSMF010000210         2859 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_37999_length_2859_cov_22.850214, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000210 DAOSMF010000000
VERSION     DAOSMF010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37999_length_2859_cov_22.850214"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1606
                     /locus_tag="PLT63_11170"
     CDS             <1..1606
                     /locus_tag="PLT63_11170"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF006821.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HOD99322.1"
                     /translation="FPRVARRIYEEARQSQRLIAGRSLEELKQIALRQEGVIQTQFGS
                     VAADSEPMSRSAPHTRNSIDHPFGEAEEALADQAAAVLSRERVIALDTIISDSRDGVT
                     ARFLIPEPYAQLAYGLKLLFDYPAPRTVEDPTYTIIYFTDEAFEFNKKKKLVDKDIDV
                     RLWMGSRRGEQVKICRNTIYLGEGKKGVFQFEDWRVKAIDKSGIFLHTGARRDLVWVY
                     DPETERPELKEIVTAISGLTATGKTTTLCRKFAKLPRESSEMIGDDGGTLAFDGSYAA
                     FEVGGLYVKTEGLDESQPEILRAAESRDAFLENVAVTKYPYIPDFRDITKTGNARAVV
                     TRDHLEIAAPGLRADKVHYIVMLTRNPLANVISRLSPEQATMQFIYGESIESTGGNPD
                     EAGKFKRVVFLDPFLTGDRLEHALIFYEIVKRNGIQCYLANTGHIGPEELKVTLRQSL
                     AAYNDLVRTQLRFSREGDCLGYRYPIKCDRANLDQMNAHRLFTDRELTRRRVEDFFRG
                     RRAFLEEFESRYGRIPAPIRESLPYE"
     gene            complement(1759..1974)
                     /locus_tag="PLT63_11175"
     CDS             complement(1759..1974)
                     /locus_tag="PLT63_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99323.1"
                     /translation="MIVINDEATPCGCCGKLPAVIYCDGCQAPLCVSCRTFDLWGYGC
                     GHVDSKAFCPRCLRDPAVNPYNGFPDK"
     gene            2108..>2859
                     /locus_tag="PLT63_11180"
     CDS             2108..>2859
                     /locus_tag="PLT63_11180"
                     /inference="COORDINATES: protein motif:HMM:NF016952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016603 - glutaminyl-peptide
                     cyclotransferase activity [Evidence IEA];
                     GO_process: GO:0017186 - peptidyl-pyroglutamic acid
                     biosynthetic process, using glutaminyl-peptide
                     cyclotransferase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HOD99324.1"
                     /translation="MVAKRKFILILLLAGMFGAASVISTTIPTAHTAEVHTSAFPTPA
                     FFPVTATLPTSAVFPASGTAAATYAATGTVTPAANVPPTEYRYRVVRAYPHDPGAFTQ
                     GLAWVDGRLYEGTGREGESSLRRVDLTTGRVLQERRLDRHHFGEGITVWKDRIIQLTW
                     RSRQGFIYDRDSFRPRGVFHYDHEGWGITHDGRRLIVSDGSPVLRFYDPETFRETGRL
                     PVRDEYSGAVGGLNELEYVRGMIYANLWPTDI"
BASE COUNT          577 a          921 c          869 g          492 t
ORIGIN      
        1 attcccgcgc gtcgcccggc ggatctatga ggaggcccgg cagtcgcaac gtctcatcgc
       61 cggccgttcc ctggaggagc tcaagcagat cgccctccgc caggaagggg tgatccagac
      121 gcagttcggc tccgtggccg ctgattcgga gcccatgtcc cgttccgccc cccacacccg
      181 caacagtatc gatcatccct tcggggaagc cgaagaggcc ctggcggacc aggcggcggc
      241 ggtcttgagc cgggagcggg tcatcgccct cgatacgatc atcagcgaca gccgggacgg
      301 cgtcacggcc cgtttcctca tccctgaacc ttatgcccag ctcgcctacg ggctgaagct
      361 cctgttcgac tatccggcgc cccggaccgt ggaagatccg acctacacga tcatctactt
      421 caccgacgag gccttcgaat tcaacaagaa gaaaaaactg gtggacaagg acatcgacgt
      481 ccgcctctgg atggggagcc gccgggggga acaggtcaag atctgccgga acaccattta
      541 tttaggggaa ggaaagaagg gggttttcca gttcgaagac tggcgcgtga aggccatcga
      601 taagtcgggg atcttcctcc acaccggcgc ccggcgggac ttagtttggg tctatgatcc
      661 cgagacggaa cgcccggagt tgaaggagat cgtcaccgcc atctcgggac tcaccgccac
      721 gggaaagaca acgaccctct gtcggaagtt cgccaagctc cccagggaat cttcggagat
      781 gatcggcgac gacggcggga ccctggcctt cgacggcagc tacgccgcct tcgaagtcgg
      841 cggcctctat gtgaagaccg agggcctcga cgagagccag ccggagatcc tccgggcggc
      901 ggaatcaagg gacgccttcc tggaaaacgt ggcggtgacg aagtatccct atatccccga
      961 tttccgggac atcaccaaga cgggaaacgc ccgggccgtg gtcacccggg accatctgga
     1021 gatcgccgca ccgggcctgc gggcggacaa ggtccattac atcgtcatgc tcacccggaa
     1081 ccccctggcc aacgtcatca gccgcctctc gccggaacag gcgacgatgc agttcattta
     1141 cggcgaatcc atcgagagca ccgggggcaa ccccgatgag gcgggaaagt tcaagcgggt
     1201 ggtcttcctc gaccccttcc tcaccgggga ccgcctggaa cacgccctga tcttttatga
     1261 aatcgtgaag agaaacggta tccaatgcta tctggccaac acggggcata tcggcccgga
     1321 ggagttgaag gtgaccctgc gccagtccct ggcggcctac aacgatctgg tacggacgca
     1381 actgcggttc agccgggaag gcgattgcct gggttaccgc taccccatca agtgcgaccg
     1441 ggccaatctc gaccagatga acgcccaccg cctcttcacg gaccgggagc tgacgcgacg
     1501 gcgggtggag gacttcttcc ggggccggcg ggccttcctg gaggagttcg aaagccgcta
     1561 cggccggatc cccgccccga tccgggaatc cctcccctac gaatagcaac cgcaatgggg
     1621 tcaggtcttt aaaattagca tttttaccgc cggggcaaag aagcgacgcg ctttaccacc
     1681 gccggcgccc ccggatgcta aaattacgaa ccgtaaatca gaggcacgga ttttcagctc
     1741 cggctctcta ctcgtcctct atttgtcggg gaaaccattg tagggattga cggcgggatc
     1801 acggagacag cgcgggcaga aggccttgct gtccacatgg ccgcacccgt aaccccagag
     1861 atcgaaggtc cggcaggaaa cgcagagcgg cgcctggcag ccgtcgcagt agatcaccgc
     1921 cggcagcttg ccgcaacagc cacagggcgt tgcttcgtcg ttgatgacta tcatgggggg
     1981 cttcctaaca cagaacaagg gagaacacaa gccaagaccc ggggtaattt ccgttgcggc
     2041 cgggagtaag aacaaggagc gatcgattcc cctcatatcg tcggcaatcc ggccgggggt
     2101 gctggccgtg gttgcgaaaa gaaaatttat cctcatcctg cttttagccg gcatgttcgg
     2161 agccgcctcc gttatctcta caaccatccc caccgcccac accgcggagg tccacacgtc
     2221 tgcattcccg acacctgctt ttttccccgt aaccgctacg ttgccgacct ccgctgtctt
     2281 cccggcatcc ggtacggccg cggccaccta tgccgccaca ggcaccgtaa ctccggccgc
     2341 gaatgtcccg ccgaccgaat accgttaccg ggttgtccgc gcttatcccc acgatcccgg
     2401 ggccttcacc cagggcctgg cctgggtgga cgggcggctt tacgagggca cgggccggga
     2461 gggagaatcc tccctgcgcc gggtggacct gacaaccgga cgggtcctcc aggagcggcg
     2521 gctggaccga caccatttcg gggaagggat caccgtctgg aaagaccgga tcatccagct
     2581 cacctggcgc tcccggcagg gtttcattta cgaccgggac tcgttccgcc cccggggcgt
     2641 ttttcactat gaccacgagg gctggggaat cacccacgac ggtcggcggc tcatcgtcag
     2701 cgacggcagc ccggtgctgc gcttttatga tccggaaacg ttccgggaaa cggggcggct
     2761 tccggtccgg gacgaataca gcggcgccgt gggcgggctc aacgagctgg aatacgtccg
     2821 ggggatgatt tacgccaatc tctggccgac ggacatcat
//
LOCUS       DAOSMF010000211         2856 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_38040_length_2856_cov_31.470546, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000211 DAOSMF010000000
VERSION     DAOSMF010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38040_length_2856_cov_31.470546"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="PLT63_11185"
     CDS             complement(<1..244)
                     /locus_tag="PLT63_11185"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOD99325.1"
                     /translation="MSAFLTDLPRYPFLQYALLTGLLVSVACGIIGSYVVTKRITYLA
                     GSIAHTVLGGLGAARYFQTAYGWEWLHPLYGAVFAAL"
     gene            945..1904
                     /locus_tag="PLT63_11190"
     CDS             945..1904
                     /locus_tag="PLT63_11190"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOD99326.1"
                     /translation="MSRFFTAATAALLAIFLIFGCGSGSKDTTDQGEKKAAPAKKRIA
                     LVMKTLTNPFFVAMEKGARKAEEELNIELIVKTGAKETSIEQQIAIIDELIRNRVDAI
                     VIAPASSTEIIPILKKAQDAKIVIVNIDNQIDPEPAAKMGLVDVPFISVDNEKGAYLA
                     AKFISDQVKKPTKAAILEGIRSAKNAEQRKAGAVRAFRENRHIRLAASESANWKIDEA
                     FAVTARIFKKHPDIGMIFCANDMMALGTIHYLQQQKKTGVLVAAFDALEEARAAVRQG
                     TLQITIDQQADRQGYLGVRTAVEMLAGKKPAPVTMVDVLPVHL"
     gene            2188..>2856
                     /locus_tag="PLT63_11195"
     CDS             2188..>2856
                     /locus_tag="PLT63_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99327.1"
                     /translation="MWQKFSDLSITYKLIVFLLLVVVAPVIFLSYTTYQLSKTALSEE
                     VKAFTLDFMALQKHNINLLVKQEESLINNIAGLDEIKNVLSVKIDNEYDRLSTHARIG
                     YILSNYSNISGLVSIDIFSVSGDHYHVGETLNVEEINDAQVRRLQQAAQASASPVVWF
                     GIENNVNLNSRYGKVITAAKVMKSYDIAGMKEKYLGLLLMSYDLSVFYNLFNYAGHTG
                     QYYLI"
BASE COUNT          715 a          828 c          773 g          540 t
ORIGIN      
        1 gcagcgccgc gaaaacggcc ccgtaaaggg gatggagcca ctcccagcca taagcagtct
       61 gaaagtaccg ggcggccccg agcccgccca ggacagtatg ggcgatgctg ccggcgagat
      121 aggtgatccg cttggtaacc acataactcc cgatgatccc gcaggcgacg cttaccagca
      181 gccccgtgag gagggcatac tggagaaagg gataccgggg cagatcggtc aaaaaggcac
      241 tcatcttttc ctcgtcgccc tcgcaagacc ttgtctgaaa ccggaaccat tcatgacgtt
      301 tgtaaagaag tggcccatcg ccttccctat gatcccaatg acgaatctcc aaaaagtctc
      361 cccgttgaac attgataaca tcgtaaaaaa ccaaaaaatg tcccatttgg gacggcctgg
      421 taaataaact ccgcaagcaa ggcacgtgaa tcctgaggaa tgagaagcag aggactaaaa
      481 gcaccaagcc cgctttttac gggctcatcg atgctgttcg cgagttgcca ggctgaatcc
      541 gtttcgtcgc tccggttgcc gtctgtgttt cttgccggcg cttatattac ttagtaatac
      601 cgtatctgtc aagataatgc ggacaaatca tttggtacaa aaccggacag ttccattggc
      661 tattgacaat cccggcgatt cctggcgatt ctccttgaca caaccgcccc gctcaccgta
      721 aaatatcatc atagttggaa aatttatccg atcaggaaat aatgatgaat tcgtaaaaag
      781 tcatgatgga gacggcgaag taaaaagctc cagaggcaag gcgcgcgaat cctgaggaat
      841 gaggcgtact tgtgcgtacg tcgcagtgac gaaggatgaa gcgcaacgcc gcatatggac
      901 tttttacgat gtcgtcaata atggcgagaa tagggaggaa agatatgagc agattcttca
      961 ccgccgcaac cgccgccctc ctggcaattt ttttgatttt cggatgcggc tccggttcca
     1021 aagacacgac ggaccagggg gagaaaaagg ccgccccggc aaaaaagcgg atcgccctgg
     1081 ttatgaagac cctgaccaac ccctttttcg tggcgatgga gaagggggcc cggaaggcgg
     1141 aagaggaact gaatatcgaa ctgattgtca agaccggagc caaggagacg tcgatcgaac
     1201 agcagatcgc catcatcgac gaactgatcc gcaaccgggt ggacgctatc gtcatcgccc
     1261 cggcgagttc cacggagatc atccccatcc tcaagaaggc ccaggacgcg aagatcgtca
     1321 tcgtcaacat cgacaatcag atcgacccgg agccggccgc gaagatgggg ctcgttgacg
     1381 tgcccttcat cagcgtggat aacgagaaag gggcctatct cgccgccaag ttcatcagcg
     1441 atcaggtgaa aaaacccacc aaagcggcga tcctcgaggg catcaggagc gcgaagaacg
     1501 cggaacagcg gaaggctggc gccgtgaggg ccttccggga aaatcgccat atccgcctcg
     1561 cggcgtcgga atcggccaac tggaagatcg acgaggcctt tgccgttacg gcccggattt
     1621 tcaagaaaca tccggacatc gggatgatct tctgcgccaa cgacatgatg gccctgggca
     1681 ccatccatta cctccagcag caaaagaaaa ccggcgtcct cgtcgccgcc ttcgacgcct
     1741 tggaggaagc cagggcggcc gtccgccagg gaaccctgca gatcaccatc gaccagcagg
     1801 cggatcggca gggatacctg ggcgtccgga cggcggtgga gatgctggcg gggaagaaac
     1861 ccgcccccgt caccatggtg gatgttctcc ccgtgcattt gtagcggtcc gatccgggac
     1921 gtcgccgtcg gcggcaagcg tgtcgtccgg ccgtcccgaa cccgccgctg ctcgccggga
     1981 cggctcgatc ttcatgcctc ctgttctcag gtcatgatcg ggaagcggcg gtcacgacac
     2041 gacccaaacc cagcggatcg gcgccacgga tcacggctcg tgttcaggaa gcgacggcac
     2101 gggacaacgg cgggaaacgg aaactgaaaa tcatccgcgg tcgtcatgac ggccgcgcca
     2161 atctatccgg accggactta acagtacatg tggcaaaaat tctccgatct gagcattacc
     2221 tacaaactca tcgtcttcct cctgctggtg gtcgtggcgc cggtcatctt cctcagctat
     2281 accacctacc agctttccaa aaccgccctc agcgaggagg tcaaggcctt caccctcgat
     2341 ttcatggccc tgcagaagca caacataaat ctcctcgtca aacaggaaga gagcctcatc
     2401 aacaacatcg ccggactgga tgaaatcaag aacgtcttgt ccgtcaagat cgacaatgag
     2461 tacgaccgcc tgtccaccca cgcccggata ggctatatcc tcagcaatta cagcaacatc
     2521 tccgggcttg tctccatcga tatcttttcg gtgtccgggg accactacca tgtgggagaa
     2581 acgctcaacg tcgaggagat caacgacgcc caggtccggc gcctccagca ggccgctcaa
     2641 gcttcggcct cgccggtggt ctggttcggt atcgagaaca atgtaaacct gaactcccgg
     2701 tacggcaagg tgatcactgc ggccaaggtc atgaagtcct atgacatcgc cggcatgaag
     2761 gaaaaatacc tgggcctcct cctgatgagt tacgatttaa gcgtgttcta caatttattc
     2821 aactatgccg gtcataccgg gcagtattac ctcatc
//
LOCUS       DAOSMF010000212         2802 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_39049_length_2802_cov_16.607572, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000212 DAOSMF010000000
VERSION     DAOSMF010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39049_length_2802_cov_16.607572"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(391..1149)
                     /locus_tag="PLT63_11200"
     CDS             complement(391..1149)
                     /locus_tag="PLT63_11200"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOD99328.1"
                     /translation="MKRNVMKAVGGVALALFFLVCLFVPLGDAAPKAGDNDDLKRLVA
                     NLHWLGHDAFRIDADGLVIYIDPFLLKTGGPKADLILITHEHMDHNNPGDVAKIKKDD
                     TIIVTAARGAEKLSGDIRIIKPGEDMTVRGIPIRSVPAYNTNKFRSPGVPFHPRELGY
                     VGYLITVGGVTIYHTGDSDFIPEMKGLKPDVALLPVSGKYVMTAEEAVAAAAAINPRL
                     AVPMHVGGPVGSISAAEDFRVRATVPVMVLPLEK"
     gene            1334..2563
                     /locus_tag="PLT63_11205"
     CDS             1334..2563
                     /locus_tag="PLT63_11205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOD99329.1"
                     /translation="MIIKNEKKMFIHGMIPIGVTTLEGGCFLSVCEAWLDFTGGTRTE
                     VIGRSWVDTGFVTKNQYDVFLAELAKKNQVENLEMTVRDKNGSVVLGLFNAAVLTLAD
                     RKCLWTVMTGTMERREAYGALLKSEQKYRLLVENINEGIWQIDKDAFTTFVNQYMAEM
                     LGYTVAEMLGRHLFAFMDGENIAAARSNLARRQAGIKERLDFVFLRKDGSPLYTSLMT
                     TPIIDKEGNYLGAIAGVQDITGRISVEEELNKHRLYLEEMIRERTVELESKNVTLQEL
                     NTTLKVLLAQRDEDKRDLEERFILNVRNLVLPYVEQMKKGKLEVEQKSYLEIIETHLK
                     EIATPLLKNIRRFNLTPREMRVASLIKSGRTTKEIAKVMGSAPGSIDVHRKNIRKKLG
                     LNNKKANLLSYLESLDQ"
BASE COUNT          686 a          760 c          767 g          589 t
ORIGIN      
        1 gaggtattga catagacctg ccgggccagt tgccggataa aatggcgctc gaaatggccg
       61 cggaaaacgg ccatgggaat caagaaccac aacaggtcga agtggaatag aaagacctgg
      121 ccgatgctta tatccggccg ggcacgcgta cggggatgga tcaaaatgaa agtcgcaaaa
      181 tcgcgcttca gatccataac atatcgaaat aatgggattt atttttaaaa aaaccgctga
      241 gccccacggc tgcattgggt tccgttttcg ctcaattata tcaaggagtt aaatgctatc
      301 tgattaagat aatttttttt gctccccccg gttgccttcc aaaaacaccc ccccggaggt
      361 ttggtgagaa aaggttatgt cgatctctcc ctacttttcc agcggcagca ccatcaccgg
      421 caccgttgcc cgcacccgga aatcctccgc cgcgcttatg ctgcccaccg gaccgcccac
      481 atgcatgggc acggcgaggc gcggattgat ggcggcggcc gcggcgacgg cctcctcggc
      541 ggtcatgacg tatttaccgc tcaccggcag gagggccacg tcgggcttga gccccttcat
      601 ctccgggatg aaatcggaat cgccggtgtg atagatggtc acgcccccca cggtgatgag
      661 gtagcccaca tagcccaact cccggggatg gaagggaacg ccggggctcc ggaacttgtt
      721 ggtgttgtat gccggcacgg agcggatggg gatgccccgg acggtcatat cctctccggg
      781 cttgatgatc cggatatcgc cggagagctt ttcggcccct ctggccgccg taacgatgat
      841 cgtgtcgtcc ttcttgatct tcgccacatc accggggttg ttgtgatcca tgtgctcatg
      901 ggtgatgagg atcagatccg ccttgggtcc ccccgttttc agcaggaacg ggtcgatgta
      961 gatgaccagg ccgtcggcgt cgatgcggaa ggcgtcgtgg cccagccagt ggagattggc
     1021 caccaggcgt ttgagatcgt cgttatcccc cgctttcggg gccgcatccc ccagcgggac
     1081 gaataagcag accaggaaaa acagggccag cgccaccccc ccaaccgctt tcatcacgtt
     1141 acgcttcata tgggctcctc cttattatct gtattgcctg tttcgatgca tacggaccct
     1201 ttataacata gcaacagggc gataataaag accgttttgt cccgtgataa atgtcatcaa
     1261 aaaaacgaca ttgcgccatc atgaagaaag tgatacgttt ttaagcattc acttccatcc
     1321 gcggactacc ggtatgatta taaaaaacga gaagaaaatg tttatccacg ggatgatccc
     1381 catcggcgtc accaccctgg aaggcggttg tttcctctcg gtctgcgaag cctggctcga
     1441 cttcaccggg ggaacacgca cggaggtcat cggccgctcc tgggtggata cgggtttcgt
     1501 tacaaaaaat caatacgatg ttttccttgc agaactggcc aagaagaacc aagtggaaaa
     1561 cctcgaaatg acggtgcggg acaaaaacgg ttcggtggtc ctgggcctgt tcaacgccgc
     1621 cgttctgacc ctggcggacc gaaagtgcct gtggacggtc atgacgggga ccatggaacg
     1681 ccgggaggcc tatggggccc tcttgaaaag cgaacagaaa taccgcctgc tggtcgaaaa
     1741 catcaatgaa gggatctggc agatcgacaa ggacgccttt accaccttcg tcaatcaata
     1801 catggcggag atgctgggct ataccgtggc ggagatgctg gggcgccatc tcttcgcctt
     1861 catggacggg gaaaacatcg ccgccgcccg gagcaatctc gcccggcggc aggcggggat
     1921 caaggaacgc ctcgatttcg tctttctccg caaggacggg tcccctctgt atacctctct
     1981 gatgaccacg ccgatcatcg acaaagaggg gaactacctg ggggcaatcg ccggcgtgca
     2041 ggatatcacc gggcgcatca gcgtggaaga agaactcaac aaacaccgcc tctacctcga
     2101 ggagatgatc cgggaacgga ccgtggagtt ggaaagcaag aacgtcacct tgcaggaact
     2161 caatacgacc ctcaaggtgc ttctggccca aagagacgag gataaaagag atttggagga
     2221 acggttcatc ctgaacgtcc ggaacctcgt cttgccatac gtggagcaga tgaaaaaggg
     2281 gaaactggag gtggaacaga agtcctatct cgagatcatc gaaacccacc tgaaggaaat
     2341 cgccacgccc cttctgaaga atatccggcg tttcaacctc acccctcggg agatgcgggt
     2401 cgcttccctg atcaaatccg gccggacaac caaagaaatc gcgaaggtca tgggctccgc
     2461 ccccggctcc atagacgtac accggaaaaa tattcggaag aagctgggat tgaacaataa
     2521 gaaggctaat ctcctctcct atctcgaaag cctcgatcaa taggtatttt atcaccccat
     2581 tatttatctg aaaatacacg attgacattg atgtggttat ccgctcatat aatgagtcag
     2641 ttcaaaatta cagtcccgat ggttatagga gtttctcggt tcgatatgga agattatcga
     2701 tgtccccact gtgggcgccc ggtaaagata ttgaaatacg gccatggctg ggtgggtatg
     2761 tgttgcgacc ggatcgttta caatgccccc aagccatacg ga
//
LOCUS       DAOSMF010000213         2672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_41587_length_2672_cov_23.703477, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000213 DAOSMF010000000
VERSION     DAOSMF010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41587_length_2672_cov_23.703477"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="PLT63_11210"
     CDS             <1..672
                     /locus_tag="PLT63_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99330.1"
                     /translation="LEEFWGESVTNHVKMQKAALFVGVKETGIELEVMPAESYEAEKS
                     APGRVGAVVCNLVVKEKPHTVFLPSPYAEVLKRIYENAKRKRQLEAATESLPAAVRTR
                     HAVTYIASAGVLRISVFEAGGDVGEVIAGLVDKYAAAGAVVFQVLLPLDKAYSGALTE
                     ALNKQGFFFSALVMRWFDADGLLLQKLAHPTDYDAIQIYSDFAKELLRFVIQDRMRVE
                     APTAG"
     gene            complement(1484..>2672)
                     /locus_tag="PLT63_11215"
     CDS             complement(1484..>2672)
                     /locus_tag="PLT63_11215"
                     /inference="COORDINATES: protein motif:HMM:NF015057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HOD99331.1"
                     /translation="KRRVRRLHLRPPLLFIEGPGRQEAGSQQQGQHKDRGRRDRFSLR
                     NYHAIRRDGSIMITMKMAFSCFVALFTMSSSLTIRRGFYILPPHEYRLRKSHRHNPQG
                     RETIRTQEALRQGIPGRVLLGLRDEVTMKKSSATRIFSSFAAPSMDMRVHWKRVAAIS
                     ICLLLILPGKIHAESFSEELWKLGYADYQQILHMPFNRELSNGVLDEKIFKSYIIQDY
                     FFLQNFRKTYGILLFKSPDEEGTQFVLDSIKAIDEEIASIHQRYFKKFNISRQDLSTA
                     SVYPSTEFYNSFLIKTATLEPFEVGLTAMLPCHRIYYQLGVDMKKAGKREGNKYQEWI
                     DGYAEESWETSATKQFADLIDLYSLRTSVETRERMKQAYRKAVKLEYLFWDGVYRDVK
                     WTP"
BASE COUNT          596 a          666 c          764 g          646 t
ORIGIN      
        1 ctggaagaat tttggggcga gtcggtgacc aaccatgtaa agatgcagaa agccgccctc
       61 tttgtgggtg tcaaggagac gggaatcgag ctcgaggtga tgcccgccga gtcctatgag
      121 gcggaaaaat cggcccccgg acgggtgggc gctgtggtgt gcaacctcgt cgtcaaggaa
      181 aagccccaca cggtctttct cccctccccc tatgcggagg tgctgaaaag gatctatgaa
      241 aacgccaaga ggaagcgcca gttggaggcc gccacggagt ctctcccggc ggcggtccgg
      301 acccgccacg ccgttaccta catcgccagc gccggggtct tgcggatcag cgtctttgag
      361 gcgggcgggg acgtagggga ggtgatcgcc ggcctggttg ataaatatgc cgccgccggg
      421 gcggtggtgt ttcaggtgct cctgcccctg gacaaggcct atagcggggc gctgacggag
      481 gccttgaaca agcagggctt tttcttttcc gccctggtga tgcgctggtt cgatgccgac
      541 ggcctgctcc tgcaaaaact ggcgcacccg acggattacg atgccatcca gatctactcc
      601 gattttgcca aggagctgct cagattcgtc atccaggatc ggatgcgggt ggaggcgccg
      661 acggcggggt aggaggggcg gcatatcctg cccccggcgt gaaagcggta atcggggggt
      721 catagtgaat cgggatccac gtcccccggg gcgtcttatc attccctacc ccccccatgc
      781 ccggcacagg cgcaacgaaa gatgaaaata tccagatagt tgtggacatt ctcatataaa
      841 tttgaatgaa gttgctcaga ggcgggcggc atgacggtgc ggccccttta agacggaagg
      901 ggtgtctgcc ctcgcgatgg aatcgcgact gatgggcgtt accgcgacga tgggtttaat
      961 gaggggatta atgcggccgg cggtgaaaaa aagaagcccc tcaaagaatg cgccttcgtt
     1021 tgaggggctg aagagatccg cctatgccgt cttgtctgcc gtctggagtc catcatttgc
     1081 atacttggtc ttccccgggt gatcatcacc actaagaccc tgcggttttc gtttcgttaa
     1141 acatccagta gaatagactc aatacggaca tcgcagattc tctattcatc gattattttc
     1201 gggtttgtcc tctgaaagag cgtttataag tgtaatttat cgatgccgat gtaaaatgtc
     1261 aagggggaga tcgggggggg caagagtgaa tagaaatctc cggtttttct gttctttgat
     1321 ggaagctgat ttagaacaag gggagaccgg ctgcggatct ccgggttgta tgcgtcccag
     1381 gaaggtcttg ccgaagcaac gatagttatt gatcataacg gctgcaatgg cgggaatgac
     1441 gccccgggcg gtatggatgc cccatgagtc atcgcctcgt tcttcacggc gtccatttta
     1501 catcccgata gacgccgtcc cagaataaat attccagctt tacggccttt ctgtatgcct
     1561 gtttcattcg ttcccgggtc tccaccgatg ttcgtaagga ataaagatca atcaaatccg
     1621 cgaattgctt ggtagcgctg gtttcccagg attcctcggc atagccatct atccattcct
     1681 gatatttgtt gccctccctt tttcccgcct ttttcatgtc cacgccgagt tggtaatata
     1741 tccggtggca cggcaacatg gcggtcaaac cgacctcaaa aggttcaagc gtcgcggtct
     1801 tgatgagaaa ggaattataa aattcagtgc ttggatatac gctggcggtc gaaagatctt
     1861 gacgggagat attgaacttt ttaaagtacc gctggtgaat gctggctatt tcttcatcga
     1921 tggcctttat tgaatccaga acgaattgcg ttccttcttc gtcgggagac ttgaaaagca
     1981 ggatgccgta agttttccgg aaattttgca ggaagaagta atcctggatg atgtagcttt
     2041 taaagatttt ttcgtcgaga acgccgttgg acagttcgcg gttgaagggc atgtgcaata
     2101 tttgctgata atcggcataa ccgagtttcc aaagctcttc cgaaaagctt tcggcgtgga
     2161 tctttccggg gaggatcagc agcaggcaaa tcgatattgc cgcgacccgt ttccaatgaa
     2221 ccctcatgtc catgctaggg gcggcgaaag acgaaaatat tctggtggcc gaggactttt
     2281 tcatggtaac ctcatcacgg agaccaagga gaactcgtcc gggaataccc tgacgcaagg
     2341 cttcctgggt gcggatggtc tcccttcctt gcggattatg tcgatggctt tttctcaggc
     2401 ggtattcatg gggaggtaag atataaaaac ctcggcgaat agtcaaggat gacgacatcg
     2461 taaaaagcgc cacgaagcat gaaaatgcca ttttcatggt gatcataatc gaaccatccc
     2521 gtctgatcgc atgatagttt ctcaacgaaa aacggtccct acggccgcga tccttgtgct
     2581 ggccctgctg ctgacttcct gcttcctgtc tgccaggccc ttcgatgaac agaagtggcg
     2641 ggcgcaagtg gagtctgcgg acccggcgct tc
//
LOCUS       DAOSMF010000214         2652 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_41975_length_2652_cov_21.299191, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000214 DAOSMF010000000
VERSION     DAOSMF010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41975_length_2652_cov_21.299191"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /locus_tag="PLT63_11220"
     CDS             <1..766
                     /locus_tag="PLT63_11220"
                     /inference="COORDINATES: protein motif:HMM:NF016083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF401 family protein"
                     /protein_id="HOD99332.1"
                     /translation="PFTLAALAGGYLFIMKKVKKSVAETVAGGNGPLPVSELLYGSGP
                     IILLIVISIAGSVLLPFQGFSGSLASLLAMLAGLILATAMAFRGCGEAWKDTLKFLGK
                     PHAWNMILLVISIQVFSAVLQYPLGASGKTIVAVMRDEFVAYGIPIVLLIAVIPFISG
                     MATGVAFGFVGASFPIVFALIGENPGLHVAAAVTALAYASGYAGMMLSPLHICYVVTC
                     EYFQTTLWRTYRYIIGPTVIMIFSAIVLSIVYYHLF"
     gene            930..1982
                     /locus_tag="PLT63_11225"
     CDS             930..1982
                     /locus_tag="PLT63_11225"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HOD99333.1"
                     /translation="MIVFLSFSNLGAETKPSVAVLDFESMGTEDYLGKAVAEIIRTTL
                     VSNKNYLVVERAQISKALSEQKFQQSGIIDDRSAVEIGKVLGADLIIVGSVVRIGNAY
                     TINSRMIDVKTGEAKLGKNVTGTNLNLITAMSNELVENLFGGDEKRTKKNDTGREKAI
                     AHRATQSQHDSGYYVNLDNWEILDGQWYAENGAIVGSGGHLILKDEFRDYIFEVRVEH
                     LSGLKSGVGIGTRCTVVPGGKKTFRNHTSINQGYAFNFGFNRTYNIYSGLSGNWYPMN
                     PSWKRYDWQYSALFREDINNIRIEAKGKNCKIFVNDSLLVEYSDSSHFQGAPLLWVQD
                     PAERIIFSDMKVLILR"
     gene            complement(2162..2407)
                     /locus_tag="PLT63_11230"
     CDS             complement(2162..2407)
                     /locus_tag="PLT63_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99334.1"
                     /translation="MGSILRNTAGVINFVIPRDGVVVVAYDDGRTSVADIVKALEKGR
                     FKLRRAPAHVQTVVSPPPAASPAPPTAPGLPLYRTRR"
     gene            complement(2423..2551)
                     /locus_tag="PLT63_11235"
     CDS             complement(2423..2551)
                     /locus_tag="PLT63_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99335.1"
                     /translation="MIDHAGIKALALAAALLWLGAMPAPAPAANRTVQFQIAGCHT"
BASE COUNT          617 a          718 c          687 g          630 t
ORIGIN      
        1 cccctttacc ctggccgccc tagccggcgg ttatctcttc ataatgaaga aggtgaaaaa
       61 gagcgtcgcc gagacggtcg ccgggggaaa cggtcccctg cccgtttccg agttgttgta
      121 cggaagcggc cccatcatcc tgctgatcgt catctccata gccggatccg ttcttcttcc
      181 tttccaaggc ttttccggga gccttgccag ccttctggcc atgctggcgg ggcttattct
      241 cgccacggcc atggctttcc ggggttgtgg cgaagcctgg aaggatacgc tgaaattcct
      301 cgggaaaccc catgcctgga acatgatcct cctggtgatc agcatccagg tattttccgc
      361 cgtcctgcaa tatcctctgg gggcatcggg aaaaaccatt gtggccgtca tgcgggatga
      421 gtttgtcgcc tacgggattc ccattgtcct cctcattgcc gtcattccct tcatttccgg
      481 gatggcgacg ggggtggcct tcggatttgt cggagccagt tttcccatcg tttttgccct
      541 cattggtgaa aatcccggtc tgcatgtcgc cgccgccgtc acggccctgg cttacgcctc
      601 cggttatgcg ggcatgatgc tttccccgct ccacatatgc tacgtcgtaa cctgcgaata
      661 ctttcaaaca accctttggc ggacctatcg ttacataatc ggcccgacgg ttatcatgat
      721 cttttccgcc atagttcttt ccatcgtcta ttatcacctc ttctagaggg cggcgattat
      781 caccttaggg atttggggac cattcgcatt attccgtgcc gttctccata aaatatgcta
      841 tgtataccaa taattgcaga caaggataaa acattgacac gaggttttca ttgaataagg
      901 gctgcctata tagcgctctc gcggcggtaa tgatcgtatt tttgtctttt tcaaacctgg
      961 gcgccgaaac caagccctct gtcgccgtcc tggattttga aagcatggga actgaggatt
     1021 atctcggcaa ggccgtggca gaaataatca ggactacgct ggtaagcaac aagaattatc
     1081 ttgtcgtaga acgtgcccag ataagcaagg ccctctccga gcagaagttt cagcaatccg
     1141 gcatcatcga tgacagaagc gccgttgaaa tcggcaaggt tctgggcgcg gatctcatca
     1201 ttgtcggatc ggtagtaaga atcggcaatg cctataccat aaattcccgg atgatagatg
     1261 taaaaaccgg cgaagccaaa ctgggaaaga atgtaaccgg caccaatctg aatctgatca
     1321 ccgcgatgag caatgaactc gttgaaaacc tctttggtgg ggacgagaaa cggacaaaaa
     1381 aaaatgacac cggcagggaa aaagcgatcg cccatcgggc gacgcaaagt cagcacgact
     1441 caggatacta cgtgaatctt gataactggg aaattctcga cggccaatgg tacgctgaaa
     1501 acggcgcaat tgtgggaagc ggcgggcatc tcatcctcaa ggatgaattc cgggattata
     1561 tctttgaggt aagggtggag cacttgagcg gtctgaagtc gggcgtcggc atcggcacaa
     1621 gatgtaccgt cgtaccgggt ggaaagaaaa cgtttcgcaa ccatacctca atcaatcagg
     1681 gctatgcctt taactttggg tttaacagaa cttataacat atacagtggg ttaagtggaa
     1741 actggtatcc catgaaccca agctggaaac gatacgattg gcaatattcg gctttattta
     1801 gagaagatat aaacaatatc cgtattgaag caaagggtaa aaactgtaag atttttgtca
     1861 atgacagcct tctggtcgaa tattcggact ccagccattt ccagggcgca cctttactct
     1921 gggttcaaga tccggcagaa agaataatat tttcggacat gaaggtgctc atcttaaggt
     1981 gatgacgaca ttaccccctc ccttattcaa agggagggaa ccgtgagacc cttttagtga
     2041 tgtgaaaaaa acgttgccag gaaattccgt tcctcatccg aaaggacatg gatcatcacc
     2101 tcatggctga ttattttccg tggcatgatc gcaaaaacaa gtgaatccgc ctatgcggcc
     2161 atcagcgtct ggttctgtaa aggggcagtc cgggcgcggt cggcggcgcc ggggaagccg
     2221 ccggtggtgg agagacaacc gtttgcacat gagccggcgc cctccggagt ttgaatctcc
     2281 ctttttccag cgccttgacg atatccgcca ccgatgtccg cccgtcgtcg taggcgacca
     2341 cgaccacgcc gtcccggggg atgacgaaat tgatcacccc cgcggtattt ctcaggatgg
     2401 accccatcct ggcccggtct cctcacgtgt ggcagcccgc tatctggaac tgcaccgtcc
     2461 ggttcgccgc cggcgccggc gccggcatcg cgcccagcca cagcagcgcc gccgccagtg
     2521 cgagggcctt aatcccggca tgatcgatca tgtgttcctc ccgttgtcac cgcaccgttc
     2581 cctcccgacc ggaggaggca accttgacgg catcgtaaaa agtccggatg cttcgttgcg
     2641 cttcatccct cg
//
LOCUS       DAOSMF010000215         2637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_42299_length_2637_cov_17.498064, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000215 DAOSMF010000000
VERSION     DAOSMF010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42299_length_2637_cov_17.498064"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1457
                     /locus_tag="PLT63_11240"
     CDS             <1..1457
                     /locus_tag="PLT63_11240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HOD99336.1"
                     /translation="VNFDKNGDTNQAAAISLYAILSFIPLFILTILMANEILGAYPRI
                     QQQLVEGVKNLSPYFSDPLLKQLGGIEEKKGVLGWIGIITLIWFSSMIFGAIETAFHQ
                     IFRSHRHRNYLWSKMLAIAMIPMGWAVAIASIFITSLAALITRQPLLAEKILPHFPML
                     QGFFFQYGIPYLVTVLFFTIVYKAIPTVKIPLGVAVAGGAIFSALMELAKHFFTWYVA
                     NYTRYNIIFGSLEAVVILLIWIFYVALILLFCAEIMASFMRRDLLLLQKAFFTSRKKT
                     DLNVNKWLNIDERLFHKFGRLYSQDSYIFREGDPSRDLFYILSGRIQLEKTVGAVKKV
                     LSDMGPGAYFGEIAALIESPRTASAKVYEVAEVAVIDGETFHKLLRESDHAALLMLKE
                     FSRRIKHTNEILEGATHSWLCLVMIMYFLKNDPLPRNRNFLEELASITGKDANEIEET
                     AQELIQAGVLKPEGDRIVAFDRERAWKVAFRKTG"
     gene            complement(1441..>2637)
                     /locus_tag="PLT63_11245"
     CDS             complement(1441..>2637)
                     /locus_tag="PLT63_11245"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOD99337.1"
                     /translation="NLVAGPGEEFLLNLANNCGTGIREGGHPGGGEILDAAVSRADSL
                     IPAVRSIQKATQDMTVGRDPTTTVDPTALHGAPVSQDAPASRSCWSDCGPALGWTGLP
                     DEGTAGTGVETRSAGASSCPDYRAFPSDLYLAPGPVLPYSAAAGCWWRRCRFCPETAE
                     RNPYRSLSAEKVFADLNRLKKEMNPVLIHFLDNAMSPALLAALARHPVGVPWYGFARI
                     TEQLAAPKFCRRLRDSGCIMLKLGLESGDQRVLDALDKGIDLKTASRVLWSLKAAGIA
                     AYVYLLFGTPAEDREAARRTVEFVAAHSTAIGFLNIALFNLPAFAAAVSGLATRTFSD
                     GDLSLYVDFDHPRGWSRREVRRFLAGEVKRHPAIAAILRRTPPVFTSNHAPFTAMGGG
                     SFTPSS"
BASE COUNT          543 a          827 c          752 g          515 t
ORIGIN      
        1 tagtaaattt cgataaaaac ggagacacca accaggccgc ggccatctcc ctgtacgcga
       61 tcctgtcctt catccccctg ttcatcctta ctattctcat ggccaacgag atcctcggcg
      121 cctatccccg catccaacag cagttggtgg aaggggtcaa gaatctgagc ccctatttct
      181 ccgaccccct ccttaagcaa ttgggaggca tcgaggagaa gaagggagtc ctgggctgga
      241 tcggcatcat taccctgatc tggttctcct ccatgatctt cggggccatc gaaacggcct
      301 ttcaccagat attccgctcc caccgccacc gcaactacct ctggtcgaaa atgctggcca
      361 tcgccatgat ccccatgggg tgggccgtag ctatcgcgag catcttcatc acctccctgg
      421 ccgccctgat caccagacag ccgctgctgg ccgaaaagat cctcccccat ttccccatgc
      481 tgcaaggttt cttcttccag tacggcatcc cctacctggt tacggtcctg tttttcacca
      541 tcgtctataa agccatcccc acggtaaaaa ttcctctcgg cgtcgccgtt gcgggcggag
      601 cgatcttttc cgccctcatg gaactggcga agcacttctt cacctggtat gtagccaact
      661 acacccgcta caacatcatc ttcggctccc tggaagcggt ggtcatcctg ttgatttgga
      721 tcttttacgt cgccctgatc ctcctgttct gcgccgagat catggcttct ttcatgagac
      781 gggatctgct gctgctgcaa aaggccttct tcacgtcgag aaaaaaaacg gatcttaatg
      841 ttaataagtg gctgaacatc gacgaaaggc tctttcacaa attcggccgc ctctattccc
      901 aggacagcta catctttcgg gaaggagacc cgagccggga cctcttctac atcctttcgg
      961 ggcgaattca gcttgaaaag acggtggggg cggtaaaaaa ggtcctgtcg gacatgggac
     1021 cgggggccta tttcggggaa atcgcggccc tcatcgagtc cccgcgcacc gcctcggcta
     1081 aggtttacga agtggcggaa gtggcggtga tcgacggcga aaccttccac aaactcctga
     1141 gggaaagtga ccatgcggca ctgctcatgc tcaaggaatt ttcccggcgg ataaagcaca
     1201 ccaacgagat cctggaaggg gccacccatt cctggctgtg cctggtcatg atcatgtatt
     1261 ttctcaaaaa cgatcctctt ccccgaaacc ggaacttcct ggaggagttg gcgtccatca
     1321 cgggaaaaga cgccaatgag atcgaagaga ccgcccagga actcatccaa gcaggggttc
     1381 tgaagcctga gggggaccgg attgtcgcct ttgatcggga gcgggcctgg aaggtcgcct
     1441 tcaggaagac ggggtgaacg atccgcctcc cattgccgtg aacggggcgt gattggaagt
     1501 gaagacgggc ggcgtccgcc tgaggatcgc ggcgatggcg ggatgccgct tcacctcacc
     1561 ggcgaggaag cggcggacct ccctgcggga ccatccccgg gggtggtcga aatccacgta
     1621 gagggacaga tcgccgtcgg agaaggtccg ggtcgccagg cccgagacag cggcggcgaa
     1681 ggccggcaga ttgaagaggg cgatattgag aaatccgatc gccgtactgt gggccgccac
     1741 gaactccacg gtgcgccggg ccgcttcccg gtcctcagcc ggagttccga agagtagata
     1801 gacataggcg gctatccccg ccgccttcag gctccagagc acccgggaag ccgtcttcag
     1861 gtcgatcccc ttgtcgaggg catcgagcac ccgttgatct cccgactcca gccccagctt
     1921 cagcatgatg cagccgctgt cccggaggcg gcggcaaaat ttcggcgccg ccagttgttc
     1981 cgtgatccgt gcgaacccgt accaggggac gccgacggga tgccgggcca gcgccgccaa
     2041 cagggcgggg ctcatggcgt tgtcgaggaa atggatgagg acgggattca tctctttctt
     2101 gaggcgattc aggtccgcaa agaccttttc cgccgaaagg gaacggtagg gattgcgttc
     2161 cgccgtctcc ggacagaacc ggcagcggcg ccaccaacac cccgccgcgg cgctgtaggg
     2221 gaggaccgga ccgggagcga ggtagagatc actggggaag gcacggtaat cggggcagga
     2281 agaggcgcca gcggaccgtg tttccacccc ggtccccgct gtaccctcat cgggcagtcc
     2341 ggtccaccct aacgccgggc cacagtcgga ccaacatgac cgtgacgctg gcgcgtcttg
     2401 cgacaccgga gcgccgtgca acgccgtcgg atcaaccgtc gtagtcggat cacgtcccac
     2461 cgtcatatcc tgcgttgcct tttgtatcga ccgtaccgcc gggatgaggc tgtctgcccg
     2521 ggaaaccgcc gcatccaaaa tctccccgcc gccgggatga ccgccctctc ttatcccggt
     2581 cccacaatta ttcgccaggt tcaggagaaa ctcctctccc ggaccggcca ccaaatt
//
LOCUS       DAOSMF010000216         2634 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_42367_length_2634_cov_24.001551, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000216 DAOSMF010000000
VERSION     DAOSMF010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42367_length_2634_cov_24.001551"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..1115
                     /locus_tag="PLT63_11250"
     CDS             153..1115
                     /locus_tag="PLT63_11250"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HOD99338.1"
                     /translation="MPFKVIITRDFEHMSAVGADIVTGSIRQVLDRQDRFILGLATGN
                     TPTGLYKHLAKAANGGAFTSARITSFNLDEYIGLPGENPQQRTLHPESYSFFMIRELF
                     GLLRENFRATYVPWGALIDQERLEAELAAYPGDWEKQGADKGKAVIIHPDAASDYLRW
                     IRNEILDAYEQKIAATGGIDLHVIGVGGRGHVAFHEAGIPFAGNRMLLVQLDDNTIDN
                     AVADGHFPHREESPRFAVSMGAELVYEAKSVLLLANGKRKAEPVAASLLGDVNEQVPI
                     SYGKVLADRGGEMIYVLDREAAANLLAQADRIKSRNIEIDDLTG"
     gene            1311..2441
                     /locus_tag="PLT63_11255"
     CDS             1311..2441
                     /locus_tag="PLT63_11255"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOD99339.1"
                     /translation="MPPRPEPPAPVIAIGSGAIESIVRCDAPLQIGRKQIVQQEESLG
                     GSGLNYTLRLLAAGHPVLPILPVGNDGRGRLIRDSVAIAAGKITDRPELAKFLSPGRF
                     FVSRLATPRSTLLVNQGQTTVFSEKFVEGKDFRKHLEKRFHDIEHGLGIVPGGVIVGH
                     IYSDKSNDYPHVPGECTRFIIDYWQDKAPVFLNLGHSQIELGIAFWEDAIRKTSVMQM
                     NIWEYKNLMKTAGRLPSLRKIIAWFRERRINAVISMGRFGALGVHGDGKDGLVYGQAL
                     LPPERVRDATGAGDAFAAGLASALAGRASFSFEDFRVAMAEAGIWASYACTTLGGSGY
                     CPDQDKLAAFRKELGEENQNPIKIVDMKAAGPMLDMMDQLYS"
     gene            2454..>2634
                     /locus_tag="PLT63_11260"
     CDS             2454..>2634
                     /locus_tag="PLT63_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99340.1"
                     /translation="MKKLADLKLSNIITATLGILFLIITAMLILLVNQGMKQQALIEA
                     EAKARILLDRNLATHT"
BASE COUNT          585 a          790 c          771 g          488 t
ORIGIN      
        1 tgtgccgggt catggtggaa ggaccgacga acgaggaaac ggaacggtac tgccgacaag
       61 tggcggacgt ggtgcgaaag accatcggct gaccaagagg cgcccactcc gagccacaaa
      121 gagcacccat cgaccacact tgaggagaag ccatgccttt taaagtaatt atcaccaggg
      181 attttgaaca catgagcgcg gtcggcgccg atatcgtcac cgggagtatc cgccaagtgt
      241 tggaccgcca ggaccgtttc atcctggggc tggcgacggg aaacacccct acgggccttt
      301 acaaacatct ggccaaggcg gcgaatggag gcgcctttac ctccgctaga attaccagtt
      361 tcaacctcga cgaatacatc ggcctgccgg gagagaatcc ccagcagcgg actctgcatc
      421 cggaaagcta ttctttcttc atgatccggg agctcttcgg gctcctcagg gaaaatttcc
      481 gggccaccta tgtcccctgg ggcgccctca tcgaccagga gcggctggag gcggaactgg
      541 ccgcctatcc cggcgactgg gaaaaacagg gcgcggacaa ggggaaggcc gtcatcatcc
      601 acccggatgc cgcatcggat tatctgcggt ggatcaggaa cgaaatactg gatgcctacg
      661 aacagaagat cgccgcgacc ggcggcatag acctccacgt catcggcgtg ggcggccggg
      721 gccacgtggc cttccacgag gcgggtattc cctttgccgg caatcgcatg ctcctcgtcc
      781 aactggatga caacaccatc gacaacgccg tggctgatgg gcacttcccg caccgggagg
      841 agagtccccg tttcgccgtc tccatgggtg cggagctggt ctatgaagcc aaatcggtgt
      901 tgctgctcgc caacggcaaa cgcaaggcgg aaccggtggc cgcctccctg ttgggcgacg
      961 tcaacgaaca ggtgcccatt tcctacggga aggttctggc cgaccggggc ggggagatga
     1021 tctatgttct ggatcgggag gcggcggcaa atctcctggc tcaggcggac cgaatcaaaa
     1081 gtcggaacat cgagatcgat gatttgaccg gataagagga attacggagc gccggaaccg
     1141 ttgaggatga aaggaagacg gcgcaccgcc gggccgtcag gatgtttgtt cgcctgaata
     1201 gcgacccatg ccccctgggg tgccttatcc tgtagtaact tgagtgaaat tacttaagag
     1261 ggacgggaat gactttgtca cgccgctcaa caaaaatgtc ggaaaaatca atgccgccgc
     1321 gaccggaacc gccggcgccg gtaatcgcca tcggcagcgg agccatcgag agtattgtcc
     1381 gctgtgacgc ccccctgcag atcggacgca agcagatcgt ccagcaggag gaaagcctcg
     1441 ggggcagcgg cctgaattac acccttcgtc tccttgccgc cggacacccg gtactgccta
     1501 tcctgcccgt gggcaacgac ggccggggca gattgatccg ggacagcgtc gccatcgctg
     1561 ccggcaagat caccgaccgg ccggaactgg caaagttcct ctcccccgga aggttttttg
     1621 tctcccgcct cgccaccccc cgttctacgc tcctggtcaa tcagggacaa acgacggtat
     1681 tttcggaaaa attcgtcgag ggtaaagact tccggaagca cctggaaaaa cgttttcatg
     1741 acatcgagca cgggctcggc atcgtaccgg gaggagtgat cgtcggccat atctacagcg
     1801 acaagagcaa cgactatccc catgttcccg gtgagtgcac ccgtttcatc atcgattact
     1861 ggcaggacaa ggcgccagtt tttctcaatc tcggtcacag tcagatcgag ctgggcatcg
     1921 cattctggga ggatgccatc cgcaagacct cggtcatgca aatgaatatc tgggagtaca
     1981 agaacctcat gaaaacggcg ggccgtctcc cctccctccg taaaatcatc gcctggttcc
     2041 gggaacgacg gatcaacgcc gtaatcagca tgggtcgttt cggcgccctg ggcgtccatg
     2101 gcgacggcaa ggacgggctc gtttacggcc aggccctgct gcctccggaa cgggtccggg
     2161 acgccaccgg ggcgggcgat gcctttgccg ccggcctcgc ctcggcactg gcgggacgcg
     2221 cctccttttc tttcgaagat tttcgcgtcg ccatggcgga agcggggatt tgggcctctt
     2281 acgcctgtac caccctgggc ggctccggtt attgccccga ccaagacaag ttggcggctt
     2341 tccggaagga actgggggag gaaaatcaaa acccgatcaa gatcgtggac atgaaggcgg
     2401 ctggaccgat gctcgatatg atggaccagc tttacagtta aaaagacagt tgcatgaaaa
     2461 aactcgccga tctcaaacta tccaatatca ttaccgccac cctcggtatc ctgtttctca
     2521 tcatcaccgc catgctcatc ctgctcgtca accagggcat gaaacagcag gccttgattg
     2581 aagcggaggc aaaggcccgc attctcctcg accgcaacct ggccacccac acct
//
LOCUS       DAOSMF010000217         2634 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_42368_length_2634_cov_18.423032, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000217 DAOSMF010000000
VERSION     DAOSMF010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42368_length_2634_cov_18.423032"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="PLT63_11265"
     CDS             complement(<1..451)
                     /locus_tag="PLT63_11265"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOD99341.1"
                     /translation="MPSGDETPAGGREKEEMTLDLLKKEATAATSTVEMVEIVEMEED
                     GEYQRQLALFSQSSTEKGIELVRIGETIARLARRRSFLDIGAGGGHLTIPISTSFDAT
                     TVVEPNARQAAYLRRRCPQFQIINDVWDKVALGSERFDFILCSHVLYY"
     gene            complement(600..2456)
                     /locus_tag="PLT63_11270"
     CDS             complement(600..2456)
                     /locus_tag="PLT63_11270"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HOD99342.1"
                     /translation="MDERVNVKALFLGPKAENYGYFKEMLGYLMDDHSEWRRNFHPDD
                     APVVSADEQAGPDYLSTLQKTREALIELAGNLQLSSIPWFSPRYLGHMNADTLIAANL
                     GYMLTLLYNPNNCAYEGSPATTALEIEVGRQLAKLMGYDPERSWGHITSGGTVANYEG
                     LWMARNLKSIPLALKTCMPAAAAGLDDWRLRNLPSAEILALADQARAGGRFEELRRHS
                     VRGIGMRGAGLGKVLVPQSKHYSWAKAVDLLGIGQEHLISVPVRDNYRIDVAALKKII
                     DGLAAQRIPILAVVAVVGTTEEGAVDEIHEIVRLRDRYEDQGVCFYLHIDAAYGGYAR
                     SLFLDAEGRFLDYNEINRQHARQGVLHGDTDWLTLDVYEAFRSMPEADSITVDPHKLG
                     YVPYAAGAIVARDRRIIDLISYFAAYVFERTDDNPMLLGSYILEGSKAGAVAAAVWMA
                     HRVVPLNITGYGRIIGYSIEGAYRFYRSLLSSPPLVVAGRRFEVIPLSRPDINIVDYA
                     FHEQGNGSLEEMNRLNLAIYEQCSYKAGPVYLSDFIMSKTALSREEYGDAPAAFIEKF
                     GIAAGEWDRLESVYVLRSCILTPYLTSNTTYEEYWRNFMQAMERAIGKACGR"
BASE COUNT          533 a          786 c          775 g          540 t
ORIGIN      
        1 tgtaatagag gacgtgggaa cagaggatga aatcgaagcg ctccgaaccg agggcgacct
       61 tgtcccagac atcgttgatg atctggaatt gggggcagcg gcgccggaga taggcggcct
      121 gcctcgcgtt cggctccacc accgtggtgg cgtcgaaaga ggtggagatg gggatggtca
      181 gatgaccgcc gccggcaccg atatcgagaa acgacctgcg ccgggccagc cgggcgatgg
      241 tttcaccgat tcttaccagt tcgatcccct tctccgtgga ggattgggaa aacagggcga
      301 gttgccgctg gtattcgccg tcctcttcca tttccactat ttccaccatt tccacggtgg
      361 aagtcgccgc cgtcgcttcc ttcttgagca gatccagggt catttcttcc ttctctcttc
      421 ctcccgccgg ggtctcatcc ccggacggca agatgtgcgt ctgccgtaag ctggctaatc
      481 cgttgggatg caagtgcaga ggatcactgc ctgaatatcc ctcatcacca agatatattc
      541 aagcttcttc tttcccggcc caaaagagga cgggtttctg aaatggaaaa aatccttgct
      601 caccgcccgc aggccttgcc gatggcccgc tccatggcct gcatgaagtt tcgccagtat
      661 tcttcatagg tcgtgttgct cgtcaggtag ggggtgagga tgcaggagcg gagcacatag
      721 acactttcca ggcgatccca ctcgccggcg gcgatgccga atttctctat gaaagcagcg
      781 ggcgcgtcgc cgtattcctc cctgctcaag gccgtcttcg acatgatgaa atcgctcaaa
      841 taaacaggtc cggccttgta ggaacactgt tcgtagatcg ccagattcaa acggttcatc
      901 tcctccagac tgccgttgcc ctgttcatga aaggcgtaat cgacgatgtt gatatcgggc
      961 cgcgacaggg ggataacctc gaagcgacgg ccggcgacaa cgaggggagg agaggaaagc
     1021 agggatctgt agaagcggta ggccccttcg atgctgtagc cgatgatccg cccgtagccg
     1081 gtgatattga gggggacaac ccgatgggcc atccagaccg ccgcggctac ggcgcccgcc
     1141 ttcgagccct ccaggatata gctgcccagg agcatgggat tatcgtccgt gcgctcgaat
     1201 acataggcgg caaaatagga gatcaagtcg atgatccggc gatccctggc cacgatagcc
     1261 cccgcagcat agggcacgta acccagtttg tggggatcga cggtgatcga atccgcctcc
     1321 ggcatggacc ggaaggcctc gtaaacatcg agggtgagcc agtcggtatc accgtggaga
     1381 acaccctgcc gggcgtgctg gcggttgatt tcgttgtaat cgagaaagcg gccctcggca
     1441 tcgagaaaga gggaacgggc gtagccgccg taggcggcgt cgatgtggag atagaagcac
     1501 accccctgat cttcgtatcg gtctcggagc cggacgatct cgtgtatctc gtccacggcc
     1561 ccttcctccg tggtgccgac tacggcgacc acggccagga tcggtatccg ctgcgccgcc
     1621 aggccgtcga tgatcttctt gagggcggca acatcaatcc gatagttgtc ccggaccggc
     1681 acggagatga gatgctcctg gccgataccg aggaggtcca ccgccttggc ccaggaatag
     1741 tgcttcgact gcggcacgag gacctttccc agcccggcgc ccctcatgcc gatgccccgt
     1801 acggaatgcc gtcgcagctc ttcgaacctc ccccccgccc gggcctgatc ggcaagcgcc
     1861 agaatctcgg cgctgggaag gttccgcagc cgccaatcat caagtcccgc agccgcggcc
     1921 ggcatacagg ttttaagggc aaggggaatc gacttgagat tgcgggccat ccaaagtcct
     1981 tcatagttgg ccaccgtccc cccggaggtg atgtgtcccc aggacctttc cggatcatag
     2041 cccatcagtt tggccagttg cctccccact tcgatctcca gggccgtggt tgccggcgac
     2101 ccctcatagg cacagttgtt gggattgtaa aggagggtca gcatatagcc gagattggcg
     2161 gcaatgaggg tgtcggcgtt catgtgcccc agatagcggg gagagaacca cggaatggaa
     2221 gagagctgca ggttgcccgc cagttcgatg agggcctccc tcgtcttctg caaggtggac
     2281 agataatccg gccctgcctg ctcatcggcg ctgaccacgg gagcatcgtc gggatggaag
     2341 ttccgccgcc attcggaatg gtcgtccatg aggtatccga gcatttcctt gaaataaccg
     2401 taattttccg ctttcggccc gaggaacaaa gccttcacgt tgacccgttc atccatgatt
     2461 aacctcgcct ttcgtttctt cgttcttgca tattaccacc ataccacaaa cggatcgccg
     2521 cggcgacgtc ccggtccccg tattgctctt ataccatgtt tagccacggc gccgtcttcc
     2581 ggacataacc ccggaacggc gactattcgg cgggagaaga catcctcgtc aggc
//
LOCUS       DAOSMF010000218         2549 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_44271_length_2549_cov_19.133921, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000218 DAOSMF010000000
VERSION     DAOSMF010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44271_length_2549_cov_19.133921"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..359)
                     /locus_tag="PLT63_11275"
     CDS             complement(66..359)
                     /locus_tag="PLT63_11275"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HOD99343.1"
                     /translation="MAAPEDFLKPNPETVFRKEEDGAFLFDPQSGSLKYINGTGVRIY
                     ELCDGGNTRAEIVTTLKDAYPDVDEETIAADTVSFLKELAALGFLRKDGVNES"
     gene            725..1909
                     /locus_tag="PLT63_11280"
     CDS             725..1909
                     /locus_tag="PLT63_11280"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOD99344.1"
                     /translation="MEITIERVLFSLMNREPSPYLREILAVPELLPCLDKNGVAGVFF
                     QALQKSGREDLAPQDVYDALQERFRLQIARNMAGAAAATEVFHLLRRADVPFLVLKGL
                     ALAENVYPHFAMRTTSDLDILIRREDLLRADDALVRAGYHAQDSTPRRALLNPPGYLA
                     SLEYHRPGSVFSYLHLHWHLVNTSTPAGGFIKHIDMERIWNQSRVGKVAGVEVLLLRP
                     EHLVIYLCEHGLRVGHSFDRLNLICDLYYAIKSPGSRLDWELMAVEANELGLRNFVYL
                     GLKIVQAYGGKTLVGDETMEKFAPPALSVWERLFLRMQLKGRRLRGSSYLVYLAASKG
                     AFSKGRLIFRTLFPPRPILAQRSWQRVEAHPAVLYMRRIGETMAVLTRLARRPTRQAA
                     RR"
     gene            complement(1994..>2549)
                     /locus_tag="PLT63_11285"
     CDS             complement(1994..>2549)
                     /locus_tag="PLT63_11285"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOD99345.1"
                     /translation="KEGKLTEEEMDMIRRHPEAAVEILSAHRDVYPKMIRAIYEHQER
                     ANGQGYPRGLKGGEICDYAQIIGICDSYEAMTHHRPHKKPLLQTDSIKELIGSRSNLF
                     DPRVIKAFLDEISIYPVGSYVRMNNRNIGRVVATNRGNPMKPVLKLISDEKGKPIDPA
                     RIIDLRNYPILNIERSVSFDEIAP"
BASE COUNT          496 a          788 c          711 g          554 t
ORIGIN      
        1 aatgccgaca ccggagattg cagcgctcgg aaagatagag atacaggctg cccagagaag
       61 gcgtgtcagg attcattgac cccatctttc cgcaaaaacc ccagcgccgc cagttccttc
      121 aagaacgaga ccgtatccgc ggcgattgtc tcttcatcca catcgggata ggcgtccttc
      181 aatgtcgtca caatctccgc ccgcgtgttc cctccgtcac agagttcgta aatccgcacg
      241 ccggttccgt ttatgtattt gaggctgccg gattgcgggt cgaagaggaa ggccccgtct
      301 tcctcctttc ggaacaccgt ctccgggttg ggcttcagga aatcctcggg ggctgccatt
      361 attctttcgt cgttcatagc gtttttttta ccattaaccg gcaattcaaa tgatcaagga
      421 tgcatataac ccgaccccac cccagtccta cccttttagg agcgggaacg gcagggcgcc
      481 ggcgccatat cgcccccctc cttccccccc ttgaaaggga gaatgataat cagtgcgtga
      541 cctctaacta atacgaactc attttcaggt gaaaagccaa aaaagccggg ggcaattttc
      601 cccgccgact cgaagtacct ttgttttcat gcgttagtca agatctattt gctttttccc
      661 acgcccctgt tatcatggcc cccaagcgca gaggagcggt gagatacggg gaagggacaa
      721 cagtatggaa ataactattg aacgagtcct cttttccctg atgaaccggg agccgtcacc
      781 ctacctccgg gaaattctcg ccgtcccgga attactcccc tgcctggaca aaaacggcgt
      841 cgccggggtg tttttccagg ccctccaaaa gtccggccgc gaagatctgg ccccgcagga
      901 tgtttatgac gcccttcagg aacggtttcg cctgcagatc gcccggaaca tggccggcgc
      961 cgccgcggct acggaagtat tccatctgct gcgccgggcg gacgtcccct ttctcgtcct
     1021 gaaaggcctc gccctggcgg agaatgttta tccccacttc gccatgcgca cgacctcgga
     1081 cctggacatc ctcatccgcc gggaagacct gctgagagcc gacgatgccc tcgtccgggc
     1141 cggttatcac gcccaggaca gcacccccag gcgggcgctt ctcaaccccc ccggctatct
     1201 agccagcctc gaataccacc ggccgggcag cgtcttttcc tacctccatc tgcactggca
     1261 tctcgtcaat acctcgaccc ccgccggggg tttcatcaaa cacatcgata tggaaaggat
     1321 ctggaatcaa tcccgggtgg gaaaagtggc gggcgtcgaa gtgctcctcc tgcgcccgga
     1381 gcacctggtc atctaccttt gtgaacatgg gctgcgggtc ggccactcct tcgaccgcct
     1441 gaatctgatc tgtgacctct actacgccat caagagccca ggatcccgac tcgactggga
     1501 actgatggcc gtcgaggcga acgaactggg gctgcggaat ttcgtctatt tgggcctgaa
     1561 gatcgtgcag gcctacgggg gaaagacatt ggtgggcgat gaaacaatgg aaaaattcgc
     1621 cccccctgcc ctttccgtct gggagcgcct ctttctgcgc atgcagttaa aaggccgccg
     1681 gttgcggggg agcagctatc tcgtttatct ggcggcaagc aaaggggctt tcagcaaggg
     1741 gcggctgatt tttcgcaccc tcttcccgcc ccgcccgatc ctggcccagc ggtcttggca
     1801 acgggtggag gcccacccgg cggttttgta tatgcggagg attggggaga caatggccgt
     1861 gctgacccgg ctggccaggc gccccacccg gcaggcagcc cggagatgaa ggcgtcggca
     1921 ggcagcgccg cgggccagac aagggttgga ggcctacccg gccagccctg ggaggccggc
     1981 gctgcaggcg tcgtcacgga gcgatctcgt cgaaagatac gctccgctcg atgttgagga
     2041 tgggatagtt ccgcaaatct atgatccggg ccgggtcgat gggctttccc ttttcgtcgc
     2101 ttatcagctt cagcaccggc ttcatcgggt tgccccggtt cgtggccacg acccggccga
     2161 tattgcggtt gttcatccgg acataagagc ccacgggata gatggagatc tcgtccagga
     2221 aggccttgat gacccgcgga tcaaagagat tgctccgtga tccgatcaac tccttgatgg
     2281 aatccgtttg cagcagcggt tttttatggg ggcggtggtg ggtcatcgct tcgtaggagt
     2341 cgcagatacc gataatctga gcatagtcgc atatttcccc gcctttgagg ccccggggat
     2401 agccttggcc gttagcccgt tcctgatgtt cgtaaatcgc ccggatcatc ttcggataga
     2461 catcccggtg ggccgaaagg atctccaccg ccgcttccgg atgtctccgg atcatgtcca
     2521 tctcctcttc cgtcagcttt ccctccttg
//
LOCUS       DAOSMF010000219         2548 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_44300_length_2548_cov_20.054553, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000219 DAOSMF010000000
VERSION     DAOSMF010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44300_length_2548_cov_20.054553"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(141..1070)
                     /locus_tag="PLT63_11290"
     CDS             complement(141..1070)
                     /locus_tag="PLT63_11290"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HOD99346.1"
                     /translation="MSDRILPEKGLVVRHGIVEIVEHWAIAISGLVLLATGMFELPMA
                     NRYYITSLPGLGWSGDFIKALYIHYAASVIFITASVFHLLYHGIRGERGMLPQPEDIK
                     TSILVLKSFFGKGEEPLFHKYLPEQRLAYVGMAVIIAMLILSGLVKTYKNLYAPDMPL
                     TLVLWATWIHNVFFVLFILALAAHIGALVIKPNRPMFRGIFTGTVRLDYARRRHPLWI
                     AEMDPEEMGRLPEVRLPEGNVSEGREAEAGDLDSLYAEIPNDGEDGRGGPAPKMRPVA
                     EDPSELDKSESPPVPPSSPDTDAGDRENEQRDA"
     gene            complement(1063..2073)
                     /locus_tag="PLT63_11295"
     CDS             complement(1063..2073)
                     /locus_tag="PLT63_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOD99347.1"
                     /translation="MNEERKSGLNRRSFLKVAAGIASAGAGMSRPGRAIAAGEGRLAT
                     LIDLSLCDGCADRAIPACVAVCKEINKDKIPPIHEPIPVPWPRKAVEDWSRRREVFDR
                     LTPYNFIYVHRAEVEVEGRPRTVFVPRRCMHCDNPACATICPFSANHKHENGAVVIDP
                     DLCFGGAKCKSVCPWEIPQRQSGVGIYLHILPNLAGNGVMYKCDLCRERLATGKLPGC
                     IEACPRQAMLIGPRRQIFAQGEEQAKAMGGYIYGKKENGGTATLYISPVPIAALNATM
                     KKQPGRPDMKAEQRRMAETDRIGKAVLQAPLYGIAAGVVGAFLSLSRRKDRRKGGEKD
                     HE"
     gene            complement(2086..2349)
                     /locus_tag="PLT63_11300"
     CDS             complement(2086..2349)
                     /locus_tag="PLT63_11300"
                     /inference="COORDINATES: protein motif:HMM:NF014624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HOD99348.1"
                     /translation="MEGKRKRIMDRFKRIEGQLRGLQNMVANEGRCEDILIQVAAVTA
                     AMKRAGAAIVEAYLEECLQKARQEPEEALADFRKILTRYMDLA"
BASE COUNT          497 a          806 c          687 g          558 t
ORIGIN      
        1 ttgtcaatca tcttcttcaa tttttccgcc gaactgctca atgccatctt caatcctccc
       61 ggttagttta tcctgtttgc cgccgattta tattaaggtc ggtcatttgt caagaacgac
      121 cattctttca aaagcatcga tcaggcgtcc cgttgttcgt tctcccggtc tcccgcatct
      181 gtatcggggg atgacggcgg caccggcggg ctttcagact tatccaattc actgggatcc
      241 tccgccaccg gtctcatctt cggcgccggt ccaccccggc cgtcctcccc gtcattcgga
      301 atctcagcgt agagactatc caaatccccg gcctccgcct ccctcccctc cgacacgtta
      361 ccctcgggta acctcacctc cggtaacctc cccatctcct ccggatccat ctccgcgatc
      421 cagagggggt ggcggcgacg ggcgtagtcg aggcgcaccg tcccggtgaa gatcccccgg
      481 aacatcggtc ggttcggctt tataaccaag gcgccgatat gagccgccaa ggccaagatg
      541 aagagaacga agaagacatt gtggatccag gtcgcccaga gcaccagggt caggggcatg
      601 tcgggggcgt aaaggttctt gtaggtcttg acgagccccg acaggatcag catggcaatg
      661 atgacggcca tgcccacgta ggcgaggcgc tgctccggca gatacttgtg aaacagcggt
      721 tcttccccct tgccgaagaa gctcttgagc accagaatcg acgtcttgat gtcctccggt
      781 tggggcagca tgccccgttc accgcggatg ccgtgataaa gtaaatggaa caccgaggcg
      841 gtgataaaga tcaccgaggc ggcataatgg atatagaggg ccttgatgaa atccccggac
      901 cagccgagac cgggcaggga ggttatgtag taacggttcg ccatgggcag ctcgaacatt
      961 cccgtcgcca ggaggacgag cccggaaatg gcgatcgccc agtgctccac gatctcgacg
     1021 atgccgtggc ggactacgag tcctttttcc ggcaagatcc tgtcactcat ggtctttttc
     1081 ccctcctttc cggcggtcct ttctccggga aagagaaagg aaggccccga ccaccccagc
     1141 ggcgattcca tagagcggcg cctgcagcac cgccttgcct atgcgatccg tctccgccat
     1201 ccgtctctgt tctgccttca tgtccggccg tcccggctgt ttcttcatcg tcgcgtttaa
     1261 tgcggcaatc ggaacggggg agatataaag ggtggctgtc ccgccatttt ccttcttccc
     1321 atagatatag ccgcccatcg ccttggcctg ttcctccccc tgagcgaaga tctgtcggcg
     1381 gggaccgatg agcatggcct ggcgtgggca ggcctcgatg cagccgggaa gtttacccgt
     1441 cgccagccgc tcccggcaga ggtcacactt gtacatgacg ccgttgcccg ccagattggg
     1501 caaaatgtgg aggtagatcc ccacgccgga ctgccgctgg ggaatctccc agggacagac
     1561 ggacttacac ttggcgccgc cgaaacagag gtccgggtcg ataaccaccg cgccgttttc
     1621 atgcttgtgg ttcgccgaaa aaggacagat agtggcgcag gcgggattat cgcagtgcat
     1681 gcatcgtcgc ggcacgaaga cggtccgcgg tcggccctcg acctcgacct cagcccggtg
     1741 gacatagatg aagttatagg gcgtcagacg gtcgaacacc tcccgacgcc gagaccagtc
     1801 ctccaccgcc ttccgtggcc agggcacggg aatcggctca tgaatcgggg ggattttgtc
     1861 tttgttgatc tccttgcaga cggccacgca ggccgggatc gcccggtccg cacaaccgtc
     1921 gcaaaggctc aggtcgatca gggtcgccag gcgtccttcc ccggcggcga tcgccctgcc
     1981 gggccgggac attccggccc ccgccgaggc gatgcccgcc gccaccttaa gaaacgaccg
     2041 gcggttcagg ccgcttttcc gttcttcatt catctccatc ctccttcaag cgagatccat
     2101 gtagcgggtc aggatcttcc gaaagtcggc caaggcctct tccggctctt gccgggcctt
     2161 ttgcagacac tcctccagat acgcctcaac aatggcggca cccgccctct tcatcgccgc
     2221 cgtcaccgcc gccacttgga tcaggatgtc ctcacatcgc ccttcgttgg ccaccatatt
     2281 ctggaggccc cgcaactgac cctcgatccg cttgaaacga tccattatac gcttgcgttt
     2341 gccttccatg ttatacccct tgggggtatg ttaggcacta accccactcc tgtcaatggc
     2401 tttttttcat acccctcctt gacaagcccg ggatcgtgat tatattggcg gcgtgatttt
     2461 attagttgaa atttcaataa tttattacat acacaaagaa ataaattttt cataatggga
     2521 ggtatggcct atgaacatta gaccgctg
//
LOCUS       DAOSMF010000220         2522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_44922_length_2522_cov_27.809891, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000220 DAOSMF010000000
VERSION     DAOSMF010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44922_length_2522_cov_27.809891"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /gene="murI"
                     /locus_tag="PLT63_11305"
     CDS             <1..687
                     /gene="murI"
                     /locus_tag="PLT63_11305"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOD99349.1"
                     /translation="PYGVKSVETITRYAIEITDFLLKKQVKLLIVACNTIAAAAYEAI
                     QERSPVPVLDVIEAGASCAAATTKTKQVAVIGTPTTVNSNAYARTIHSLDPDIRVFSQ
                     ACPLFVPLVEEGWLDHPVTRLTAQEYLKPILAEHVDTLVLGCTHYPLLKSLLSDVVGN
                     AIKLVDSAEAMAEITARLLQKKGLANPLRIPPEYRFCVSDVPFRFQTIAERFLGRTLK
                     HVEVVKLPMQ"
     gene            710..943
                     /locus_tag="PLT63_11310"
     CDS             710..943
                     /locus_tag="PLT63_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOD99350.1"
                     /translation="MDIKILGPGCARCDHVEKLVTEVLAAEKIPATIEKIKDVKRFAA
                     YGVFITPALIVDNEVKCAGKIPTKDEVVRWLKK"
     gene            1051..1599
                     /locus_tag="PLT63_11315"
     CDS             1051..1599
                     /locus_tag="PLT63_11315"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOD99351.1"
                     /translation="MALVLLSWPLFPGGVAGDEPGAFPQYGAGVIQVRLYTDYFCGPC
                     RSIEPTAEALLRDLVKKQRITLTLVDLPLTRNSVLYARYFLYALNKRNELEHALKVRN
                     FLFETAAAREKSSPERIETAFKNKGIAFASFDPKALFARYNELIKEDGVHSTPTCVIV
                     KTGKKETFIGTDEVIKALKKLP"
     gene            1596..2045
                     /locus_tag="PLT63_11320"
     CDS             1596..2045
                     /locus_tag="PLT63_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99352.1"
                     /translation="MTAGATIRRSFNVILPLIGMGLLIFYSFCDTSCRYLKGDLLGME
                     LQDVGIIFMGLLLALNLPLPGRYGNMGEHLRTALISGALGGEALLVRFQIVNDTYCPL
                     CLAFGACVVVLFAVNFAAMRKPLALGAFLAGLGVFSLFFSGLVLPLY"
     gene            2312..>2522
                     /locus_tag="PLT63_11325"
     CDS             2312..>2522
                     /locus_tag="PLT63_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99353.1"
                     /translation="MGHFLTFYDVVFFNSVPFMHRRSLTSLVLLSNIKNKALGAPPMK
                     KKEAIITFKVNEDLREVISNIPNRSE"
BASE COUNT          549 a          772 c          666 g          535 t
ORIGIN      
        1 ccctacggcg tcaaatccgt ggaaaccatc accaggtacg ccatagaaat aaccgatttt
       61 cttctgaaga agcaggtcaa gctcttgatc gtcgcctgca acaccatcgc cgccgccgcc
      121 tacgaggcga tccaggagcg ctcgcccgta ccggtcctgg atgtcatcga ggccggggcc
      181 tcctgcgccg ccgcgacgac gaagaccaaa caggtggcgg tcatcggcac cccaacaacg
      241 gtgaacagca acgcctacgc ccggacgatc cacagcctcg acccggacat ccgggttttt
      301 tcccaggctt gtcccctatt cgtccccctc gtggaagagg gctggctgga tcatccggtg
      361 acccgcctga ccgcccagga atatctgaaa ccgattctgg cggaacacgt ggacaccctg
      421 gttctgggct gcacccatta cccgctcctc aagtccctgc tttcggatgt cgtcgggaac
      481 gcaataaagc tcgtggattc cgccgaggcc atggcggaga tcaccgcccg gctactgcag
      541 aagaaggggt tggccaaccc tctccggatt cccccggagt atcgcttctg cgtctctgac
      601 gtccccttcc ggtttcaaac catcgccgag cgcttcctgg gtcggaccct aaagcacgtg
      661 gaggtggtga aattgcccat gcaataagca gccaaatgag gtaatcgcga tggacatcaa
      721 gatcttggga ccgggatgcg cccggtgcga ccatgtggag aaactggtca cggaggtcct
      781 ggcggcggag aaaatccccg cgacgataga aaaaatcaaa gacgtaaaga gattcgccgc
      841 ctatggcgtc ttcatcaccc cggccctgat cgtcgataac gaggtaaaat gcgccgggaa
      901 gatccctaca aaagacgagg tcgtcagatg gctgaaaaaa tgaacggaga cgagtcgaca
      961 tcatggaagg tggcaagcac gaattttaaa gtctataaaa caaagtttaa agtccctaca
     1021 gacaagtcaa gtttaagcat cataatcgcc atggcgcttg tactcctttc ctggccgctg
     1081 tttcccggcg gcgtcgctgg cgacgagccg ggggcctttc cccaatacgg tgccggggtg
     1141 attcaagtcc gcctgtacac cgactatttc tgcggtccct gtcgctccat cgagccgacg
     1201 gcggaggcgc tgctgcgcga cctggtgaaa aagcaacgga tcactctgac cctggtggat
     1261 ctccccctca cccgcaacag cgtcctttac gcccgctatt ttctctacgc cctgaacaaa
     1321 cgcaacgaat tggaacatgc cctgaaggtc cggaacttcc tattcgaaac ggcggcagcg
     1381 agggaaaaat cgtcgccgga gcggatcgaa acggccttca agaacaaggg gatagccttt
     1441 gcaagctttg atcccaaggc cttgtttgcc cgttacaacg agctgatcaa ggaagacggc
     1501 gtccattcca cgcccacgtg cgtaatcgtg aagacgggga agaaagagac cttcatcggc
     1561 acggatgagg tcatcaaggc cctgaagaag ctgccgtgac cgccggggcg acgatccgca
     1621 ggagtttcaa cgtgatcctg ccgctgatcg gcatggggtt gctgatcttc tattccttct
     1681 gcgacacctc ctgccgctac ctgaagggcg acctgctggg catggagctg caggatgtcg
     1741 ggatcatctt catgggcctc ctgctggcac tcaatctccc cctccccggc cgctacggaa
     1801 acatgggcga acatctgcgc acggccctca tctccggcgc cctggggggc gaggcgctgc
     1861 tggttcgctt ccagatcgtc aacgatacat actgccccct gtgcctggcc ttcggggctt
     1921 gtgtcgtcgt cctcttcgcc gtcaacttcg ccgccatgag aaagcccttg gccctggggg
     1981 ccttcctggc cgggttgggc gtcttcagcc tctttttctc cggcttggtt ctccccctct
     2041 actaataaaa tccggcccca ggcgatgccg gacagattcc ttaacatctt tatatctttg
     2101 cacaacaccc atttttattg ttcccgcgca tgcggcattg gctgccggat tgacaccagc
     2161 attacgaatc gatctttttt gacggcatcg taaaaagtcc ggatgcttcg aaagcgcttc
     2221 atccctcgtc actgcgacgt acgtcccagt acgcctcatt cctcaggatg cgcgcgcctc
     2281 gcctgcggat ctttttacga taccgtccca aatgggacat tttttgacct tttacgatgt
     2341 cgtctttttt aattccgtcc cttttatgca ccggcgctcc ttgacaagtc tggtattact
     2401 tagtaatata aaaaacaaag cactgggagc gccgccgatg aagaaaaagg aagccattat
     2461 cacctttaag gtcaacgagg acctccggga ggtgatcagc aacatcccca accgctcgga
     2521 gt
//
LOCUS       DAOSMF010000221         2498 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_45458_length_2498_cov_30.713058, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000221 DAOSMF010000000
VERSION     DAOSMF010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45458_length_2498_cov_30.713058"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..614)
                     /locus_tag="PLT63_11330"
     CDS             complement(123..614)
                     /locus_tag="PLT63_11330"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HOD99354.1"
                     /translation="MTEAYGGFWRRGLAFCIDQVILNVIYLIFLLAGGLSHLLGQAAG
                     GGGPWPAAAPGAGVSQATAGFTLLYFLAVVVVTVLYFTYFVAVAGRTPGKMILGLHIR
                     PVADGSITFGVAFLRLIGYLVSGALFYLGFLWIAVDPRKQGWHDKIAGTVVIRDRGKP
                     LPP"
     gene            complement(607..1590)
                     /locus_tag="PLT63_11335"
     CDS             complement(607..1590)
                     /locus_tag="PLT63_11335"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008692.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HOD99355.1"
                     /translation="MTFPRLYWPQTLKEDGIYALDAGRRRYLSNVLRLRPGDAVTLFD
                     GSGWDFPAVIERMDDREVAVRILRREAIAAAIPSVRITLAQALPKGVKMDGIVEKATE
                     IGVIRIVPFHSARSVPRLDAAKAAARRERWEKIAVEAARRCRRPDVPAMEMIASFGEM
                     LDLAEGAGAKLAPARSGGGNRRRNHEEAAARPAAEDELFPAPLVGKGQRQQGRGVVAS
                     PSAPLPGRIIFWEEGGRDIKSILQPVGGPPPEDYFVVVGPEGGFAPEEVAAARASGFT
                     VAGLGHRILRVETASLVILTIIQYERGIFAAPDQPEGAGAVGTPRKERDRD"
     gene            complement(1590..>2498)
                     /gene="prmA"
                     /locus_tag="PLT63_11340"
     CDS             complement(1590..>2498)
                     /gene="prmA"
                     /locus_tag="PLT63_11340"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HOD99356.1"
                     /translation="QIEISLPAELSEAVTNFLMENGAQGVFQEETPEPQGGGFAEDAS
                     PETLKAYLPDDVRVENRLTSLQNYLDELGRLFPELPRPSWRTDWIAESNWGEEWKKYF
                     KPLRVTKNIIIKPTWERYSPGGHDILIEIDPGMAFGTGQHPSTRMCLQAMEDLILQDR
                     EARNRRVLDVGTGTGILGIAAAKLGAGKVVCVDIDKQAVAIAAENARMNGVEDRVDVL
                     YQDAATLAETFDLIAANLTARMLIKLRPRLIDLLSRGGFLILSGIIEQNREDIEHHFL
                     AEPFVAHRTIIEKEWRCYVLRNEGYR"
BASE COUNT          451 a          822 c          795 g          430 t
ORIGIN      
        1 ttgaactgcc gacactcgga ttttcagtcc gatgctctac cgactgagct accggcccgc
       61 gaacagatgg tccgtctatt aaatatcgct ccatttgtca aggtattttt ctccggcggc
      121 gctcacggcg ggagcggctt cccccggtca cgaatgacca ccgtaccggc tatcttgtcg
      181 tgccaaccct gcttgcgggg atcgacggcg atccacagaa aacccagata aaacagggca
      241 ccggaaacga gataaccgat gaggcgcaaa aaggcaaccc cgaaggtaat gctaccgtcc
      301 gccaccggcc ggatgtggag gccgaggatc atcttccccg gcgtccggcc ggctacggcg
      361 acaaagtagg taaaatacag gaccgtaacc acgacgacgg ccaggaagta gagcagggta
      421 aacccggccg tcgcctggga gaccccggcg cccggcgcgg cggccggcca ggggcctccc
      481 cctccggccg cctggccgag gagatgggac aatccgccgg ccagcagaaa gatgagatag
      541 atgacgttca atatgacctg atcgatgcaa aaggcaaggc cccgccgcca gaaacctccg
      601 taagcttcag tcacggtccc gctcctttcg gggcgtcccc accgccccgg cgccctccgg
      661 ctggtccggc gccgcaaata tcccccgctc gtactggatg atcgtcagga ttaccaggga
      721 ggccgtctcg acgcgcagga tccggtggcc cagaccggcc acggtaaaac ccgacgcccg
      781 ggccgccgcc acttcttccg gggcaaaacc gccttccggg ccgacgacga cgaaataatc
      841 ctccggcggc gggccgccga cgggctgcag gatggatttg atgtcccggc cgccttcctc
      901 ccagaagatg atccgtcccg gcaggggcgc agacggggaa gccacgacgc cgcggccttg
      961 ttgccgctgt cccttcccca caagcggcgc cggaaacaat tcatcctccg cggcaggccg
     1021 cgccgccgcc tcctcatggt tgcggcggcg gtttccgccc ccggatcgcg ccggcgccaa
     1081 tttcgctccc gctccttcgg ccaggtccag catttctccg aacgaagcga tcatctccat
     1141 cgccggaacg tccggacggc ggcaccgccg ggcggcctcc acggcgatct tctcccaccg
     1201 ctcgcgacgg gcggcagcct tcgcggcgtc cagccgggga acggaacggg cggaatggaa
     1261 cggcacgatc cggatcaccc cgatctccgt ggccttttcc acgatgccgt ccatcttcac
     1321 ccctttgggg agggcctggg ccagggtgat ccggacggac gggatggcgg cggcgatcgc
     1381 ttcccgtctc aagatccgga cggcgacctc acggtcgtcc atcctttcga tcacggcggg
     1441 aaagtcccag cccgatccgt cgaaaagggt gacggcatcg ccggggcgga ggcgcaggac
     1501 attactcagg tagcgccggc ggccggcatc cagggcgtag ataccatctt ccttgagggt
     1561 ctggggccaa tagaggcggg ggaacgtcac taccggtagc cctcgttgcg caggacatag
     1621 cagcgccatt ctttttcgat gatggtgcgg tgcgccacaa agggctccgc caggaagtgg
     1681 tgttcgatat cttctcgatt ttgttcgatt atcccggaca gaatgagaaa gccgccccgg
     1741 ctgaggaggt cgatgaggcg ggggcgaagc ttgatgagca tcctggccgt gaggttggcc
     1801 gcaatgaggt cgaaggtctc ggcgagggtg gccgcatcct ggtagagaac gtcaacccgg
     1861 tcctccacgc cgttcatgcg ggcgttctcc gcggcgatcg ccacggcctg cttgtcgata
     1921 tccacgcaaa cgactttgcc cgccccgagc ttggcggcgg cgatacccag gatgcccgtc
     1981 cccgttccca cgtcgaggac gcggcggttc cgtgcctccc ggtcctggag gatgaggtcc
     2041 tccatggcct ggagacacat ccgcgtcgag ggatgctgac cagttccgaa ggccatgccg
     2101 ggatcgatct cgatgaggat atcgtggccg ccgggactgt agcgctccca ggtcggcttg
     2161 atgatgatgt tcttcgtcac ccgcaggggt ttgaagtact tcttccactc ctccccccag
     2221 ttggattcgg cgatccaatc ggtgcgccag gaaggccggg gcagctcggg gaagaggcgg
     2281 cccagttcgt cgaggtagtt ctggagggag gtcaggcggt tttcgacgcg gacgtcatcg
     2341 ggcagatagg ccttgagagt ctccggggag gcatcctcgg caaaaccgcc gccctggggc
     2401 tccggcgtct cttcctgaaa cacgccctgg gcgccgtttt ccatgaggaa gttggtcacg
     2461 gcttcactca actccgccgg gaggctgatc tcgatctg
//
LOCUS       DAOSMF010000222         2467 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_46265_length_2467_cov_19.446103, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000222 DAOSMF010000000
VERSION     DAOSMF010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46265_length_2467_cov_19.446103"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..2232)
                     /locus_tag="PLT63_11345"
     CDS             complement(64..2232)
                     /locus_tag="PLT63_11345"
                     /inference="COORDINATES: protein motif:HMM:NF022783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3141 domain-containing protein"
                     /protein_id="HOD99357.1"
                     /translation="MNDSAPYETGAYRETIEQAGAYVTDTIQRSILFADALRRRGNTY
                     IEHLKNGQPPVLTFKYEMILDGREFSRPVNYSLVRISDRRARPRSPEDKEAQRRRERR
                     GKKEAPKRPIVIIDPRAGHGPGIGGSKRDSEIGMALEHGYPVYFILFYTEPMPGQTLS
                     DVEAAEVRFIEEVVKLHPYADAPAIMGNCQAGWAAALLCADRPDITGPLVLNGAPLSY
                     WAGVEGTNPMRYRGGLLGGSWLVSLMSDLGNGKFDGAHLVNNFEALNPANTVWTKQYN
                     VYKNIDTEVERYLNFEKWWGGFFLMNTEEIEFIVNNLFVGNKLEKGELEIRPGMKINL
                     RNIESPIIVFASQGDNITPPQQALNWITRVYRTTEDIKRAGQVIIYIIHEDIGHLGIF
                     VSAGVARKEHQEIIANFDMVDFLPPGLYEMIIDGDVKTGNLDFRFEERSINDILAMDD
                     GTTDEEDFCAVDRLSELLDNQYKIYLRPWVRSWSSELTGLVTRLLHPLRTQRYLLSDI
                     NPLLRPLPYAASLVKANRKPAPPDNPFVRYEYAGSDFMIQWLNQYRDARDRYIEWTFQ
                     AFYEHPWVRFFCMPVKEEEENVAAEKDQGSVPADARWQYLEEGGVAEGLIRIIMAVAR
                     KNFTIKRRLFEVGQEISMTHKVLGKLRPAEFKRIARAQARILQVDEDAALAALGKLIK
                     TKEGRSEAVTIARRIALADGRLMTEEKEVLDKIQQAFAAA"
BASE COUNT          440 a          722 c          762 g          543 t
ORIGIN      
        1 ctgtccgacg tggtgacgca gcggctgatc tgctacggga attagttcgg cggcgagttc
       61 cctttacgcc gcggcgaagg cctgctggat cttgtccagg acctccttct cctcggtcat
      121 gagcctgccg tcggccaggg cgatgcgccg ggcgatggtg acggcctccg agcgcccttc
      181 cttggtcttg atgagcttgc ccagggcggc cagggcggcg tcctcatcga cctggaggat
      241 ccgggcctgg gcccgggcga tgcgtttgaa ctccgccggt cgcagtttgc cgagaacttt
      301 gtgggtcatg gatatctcct gccccacttc gaagaggcgc ctcttgatgg tgaaattctt
      361 ccgggccacc gccatgatga tgcggatcag cccctcggcg acgccccctt cctccagata
      421 ttgccagcgc gcatccgccg ggaccgatcc ctggtctttt tccgccgcga cgttttcctc
      481 ttcttctttc acgggcatgc agaagaaccg cacccatgga tgctcgtaga aggcctgaaa
      541 ggtccactcg atgtaacgat cccgggcgtc ccggtactga ttgagccatt ggatcatgaa
      601 atccgacccg gcgtattcat atcgaacaaa gggattgtcc ggcggcgccg gtttgcggtt
      661 ggcctttacc agcgaggccg catagggcag gggacgcaac agggggttga tgtcggagag
      721 gagatagcgc tgggtccgga gcggatggag gaggcgggtg accaggccgg taagttctga
      781 cgaccaggaa cggacccagg ggcgcaggta gatcttgtac tggttgtcca gaagttccga
      841 cagccggtcc acggcgcaga agtcctcctc atcggtggtg ccgtcgtcca tggccaggat
      901 atcgtttatg gaccgctcct cgaaacggaa atccagattg ccggtcttta cgtcgccgtc
      961 gatgatcatt tcgtaaagac ccggcggcag gaaatcgacc atgtcgaagt tggcgatgat
     1021 ctcctggtgt tccttccgcg ccacgccggc ggagacgaaa atgcccaggt gtccgatgtc
     1081 ctcgtggatg atgtagatga tgacctgtcc cgccctcttg atgtcctccg tggtccggta
     1141 aacccgggtg atccagttga gggcctgctg gggaggggtg atgttgtctc cctgggaggc
     1201 gaagacgatg atgggacttt ctatgttgcg gaggttgatc ttcatccccg ggcggatctc
     1261 cagctccccc ttctccagtt tgttgcccac gaagaggttg ttgacgataa attcgatctc
     1321 ttcggtgttc atgagaaaga acccgcccca ccatttttcg aaattgaggt agcgctccac
     1381 ctcggtgtcg atgttcttat agacgttgta ctgtttggtc cagacggtgt tggcgggatt
     1441 aagggcctcg aaattgttca ccagatgagc gccgtcgaac tttccgtttc ccaggtcgct
     1501 catcagggaa accagccagg agccccccag gagccccccc cggtagcgca tgggattcgt
     1561 tccctccacc ccggcccagt aagagagcgg cgccccgttg aggaccaggg gcccggtgat
     1621 gtcgggacgg tcggcgcaga gcagggcggc ggcccagccc gcctggcagt tgcccatgat
     1681 ggctggggcg tcggcgtaag gatggagctt cacgacctcc tcgatgaacc tcacctcggc
     1741 ggcctccaca tcactcaggg tctgtccggg catcggctct gtgtagaaga gaatgaaata
     1801 gacaggataa ccgtgttcga gggccatgcc gatctccgaa tcccgtttgg agccgccgat
     1861 tcccggtccg tgcccggccc ggggatcgat gatgactatg ggccgcttgg gcgcctcttt
     1921 ctttccccgc cgctcccggc ggcgctgggc ctctttgtct tccggagaac ggggacgggc
     1981 cctccggtcg ctgatgcgca ccagggagta gttgacaggg cgcgaaaatt cccgcccgtc
     2041 caggatcatc tcgtatttga aggtcagcac gggcggctgg ccgtttttca ggtgttcgat
     2101 ataggtgttg ccgcgccggc gcagggcgtc agcaaagagg atggaacgct ggatggtgtc
     2161 cgtgacatat gccccggcct gttcgatggt ttcccggtat gcgccggttt cgtaaggagc
     2221 tgaatcgttc atgtcgtttg gcctttcgaa attaaaatcc gcgggaccat agggcaaagc
     2281 attaataatg tcaatcttat tttccgctct caagccaccc tcaccttcaa ggtgagggtg
     2341 gcttgagact attcaaccga ttgtcaaaca ttctatcacg acagtttccc tcgcctgcct
     2401 acaccacgcc gaaggccagc atggcccggg cgaccttggt gaaaccggcg atgttggccc
     2461 ccatcac
//
LOCUS       DAOSMF010000223         2466 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_46288_length_2466_cov_20.544173, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000223 DAOSMF010000000
VERSION     DAOSMF010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46288_length_2466_cov_20.544173"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="PLT63_11350"
     CDS             complement(<1..627)
                     /locus_tag="PLT63_11350"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOD99358.1"
                     /translation="MGVDNSTIPQGIHIVAKPIGPRCNLACDYCFYTEKTALFPKDEE
                     YRMPDNVLTAFITKYIAAQPTPEVEFVWQGGEPTLLGLDFFKKVVELQRPFTGIKTIR
                     NCLQTNGTLLTDAWCRFLKRNNFLVGISLDGPRQIHDRYRRDRGGKGSFSRAMKGLEL
                     LRRHGVEYNIMACVARETAAHPREIYDFFRGEGVEFIQFFPIVERIPAG"
     gene            868..2067
                     /gene="sfsA"
                     /locus_tag="PLT63_11355"
     CDS             868..2067
                     /gene="sfsA"
                     /locus_tag="PLT63_11355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HOD99359.1"
                     /translation="MYFHTDLFHEPLFTDLVPGRFIRRLNRFVIECEWGGQAVQAHLP
                     NPGRLWELLVPGRTVTLVKNAASPGRTMPYTAVAVERDGVTVLLHTTKTNDVVRFLLA
                     SKSIPGLDGAEIVRREFTLGDSRFDFLLQEGGGKILLEVKSCTLYGNSLAMFPDAVSA
                     RGRRHLEELSAHAQKGCRCGVIFLIHSPRPAFFLPDYHTDYEFARAFYKHREHLFYRA
                     LRVSWREDLRLDEKIEEAVIPWPILEREGHDRGCYLLLMRLPAATRIAVGALGTIDFP
                     AGHYLYIGSAQKDLTARLARHLRKGGKNLHWHVDYLREAADSCRALPIRTRDDLECEL
                     AAAANRIAAHAVPGFGASDCFCPSHLFFLGTLPPLHNQAFLDLVQYYRMDRLFPDPAK
                     EGDIDRY"
     gene            2128..>2466
                     /locus_tag="PLT63_11360"
     CDS             2128..>2466
                     /locus_tag="PLT63_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99360.1"
                     /translation="MMAGIKRSLWALMVLAVILWGAAAAAETTDGGKAGAAPSVDSLF
                     LGTKGENSDLLIQMFRMTTNEHKNWRQGFHPEDPPTAGSGNLASPEAAEAQRRLQEAL
                     TTLSGWMRGGS"
BASE COUNT          488 a          761 c          737 g          480 t
ORIGIN      
        1 cccggcgggg atccgttcga cgatgggaaa gaactggatg aactcgaccc cctctcccct
       61 gaagaagtca tagatttccc ggggatgggc agcggtttcc cgggcgacac aggccatgat
      121 gttgtattcc acgccgtgcc gccggaggag ttccagaccc ttcatggccc gggagaagga
      181 gcccttcccg ccccggtccc gccggtaacg gtcgtggatc tgccgtggcc cgtcgaggct
      241 gatcccgacg aggaagttgt tccgcttcag aaatctgcac caggcgtcgg taaggagggt
      301 gccgttcgtc tggaggcagt tccggatcgt cttgataccc gtaaaaggcc gctgcaattc
      361 caccaccttt ttgaaaaaat cgagccccag cagggtcggt tcccccccct gccagacgaa
      421 ctccacctcc ggagtgggct gggccgcaat gtatttggta ataaaggcgg tgaggacatt
      481 gtccggcatc cgatactctt catccttggg gaaaagggcg gtcttctccg tgtagaagca
      541 gtaatcacag gccaggttgc agcgcggacc gatgggcttg gccacgatat ggataccctg
      601 ggggatggtg gaattatcga ctcccaaaag ctgctctcct ttcctgccag cggccgggca
      661 acacggcgac gaggggcggc gacggaagcc ctaccgttgc ccctgtatca cagtcgacgg
      721 ggatattcct cccttttcaa tgatcggcgc gacgatcagg tggtaaaggc cctgggaggg
      781 ggcggggaaa ggacgaaagc tacctaatga attgcctgtt tgtccaacac ccacgaacga
      841 tgaaaatgtt caggggagat tgcgcggatg tattttcata ctgacctctt ccatgaacct
      901 ctcttcaccg acctggtgcc gggccggttc atccggcgcc tcaatcgttt cgtcatcgaa
      961 tgcgagtggg ggggacaagc ggttcaggcc catctcccca acccggggcg attatgggag
     1021 ctcctcgttc cgggccggac ggtgaccttg gtaaaaaacg ccgcgtcccc gggacgcacc
     1081 atgccctata cggcggtggc ggtggaacgg gacggggtca ccgtgcttct ccataccacg
     1141 aagaccaacg acgtcgtccg gttcctcctg gcatccaagt ccattcccgg tctggatgga
     1201 gccgaaatcg tccggaggga attcaccctg ggagacagcc gcttcgactt tctccttcag
     1261 gaaggcgggg ggaagattct cctggaggtg aaatcctgca ccctctacgg gaactccctg
     1321 gccatgttcc ccgacgccgt cagcgcccgg ggccggcgcc atctggagga gctgtccgcc
     1381 catgcccaaa aaggctgccg gtgcggcgtg atctttctca tccattcccc ccggccggcg
     1441 tttttcctgc ccgattatca caccgattac gaattcgccc gcgccttcta caaacaccgg
     1501 gaacaccttt tttaccgggc gctccgcgtc tcctggcggg aagatctccg cctggatgaa
     1561 aagatcgaag aagcggtcat cccctggccg attctggaac gggagggcca tgaccgcggc
     1621 tgctatctgc tcctgatgcg cctccccgcc gccacccgga tcgccgtggg cgccctgggc
     1681 accatcgact ttcccgccgg gcactatcta tacatcggat cggcgcaaaa ggatcttacg
     1741 gcccggctgg cccgtcacct ccgaaagggc ggcaagaatc ttcactggca tgtggattac
     1801 ctgcgggagg ccgctgattc ctgccgcgcc ctgcccatcc ggactcggga cgatctggaa
     1861 tgcgaactgg ccgcggccgc aaaccgcatc gccgcccatg ccgttcccgg cttcggggcc
     1921 tcagactgct tctgtccaag ccaccttttt ttcctgggga cccttccgcc cctgcataac
     1981 caggcattcc tggacttggt gcaatactat cgcatggaca ggctgtttcc tgatcctgca
     2041 aaagaaggcg acatcgatcg ctactgacga cccgtacata atgtcccatt tgggacggca
     2101 tcgtaaaaaa gcgataagga ggctaacatg atggcaggca ttaaaaggtc cctttgggcg
     2161 ctcatggtac tggctgtaat cctctgggga gcggcggccg cggcggaaac aacggacggc
     2221 gggaaagcgg gagcggcgcc atccgtggat tccctcttcc tcggaaccaa gggtgaaaac
     2281 agcgatttgt tgatccagat gttccggatg acgaccaatg agcataaaaa ctggcggcaa
     2341 ggcttccatc cggaagatcc cccgaccgcc ggcagcggga acctcgcctc cccggaggcc
     2401 gcggaggctc aaagaaggtt acaggaggcc ctgactaccc tgtccgggtg gatgaggggg
     2461 gggtca
//
LOCUS       DAOSMF010000224         2444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_46804_length_2444_cov_19.262871, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000224 DAOSMF010000000
VERSION     DAOSMF010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46804_length_2444_cov_19.262871"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..1112
                     /locus_tag="PLT63_11365"
     CDS             84..1112
                     /locus_tag="PLT63_11365"
                     /inference="COORDINATES: protein motif:HMM:NF014324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HOD99361.1"
                     /translation="MNRCLFFVGCLLAGIMGMLGGPEPVAAAKSYESCVEINDDALRL
                     RCYDEVTGRKTKQPDAPVSALATPPASQAPVVTSGSSVLSRMWQLDNESRRRRFAIMP
                     HRQNYILPYTYNFSQEKETYEKAKPGTELQNAEAKFQISLKMKLWEDIFGTKMDLWAA
                     YTQVSFWQVYNTEHSSPFRETNYEPELLLTYPTNIDLWGLKSRFVQIGINHQSNGRGE
                     PLSRSWNRFTANFGFERDAFNLILKTWVRIPERESQDDNPDITSYLGYGEIWAGTVWK
                     DYSLGLMFRNNLRFDENRSALQCEVIFPLIEYLNGYIQYFIGYGESLIDYNHFTNRIG
                     LGVMVKDW"
     gene            1296..2441
                     /locus_tag="PLT63_11370"
     CDS             1296..2441
                     /locus_tag="PLT63_11370"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOD99362.1"
                     /translation="MSKVALIRCESYEYGKVRSAIRHGLNLIGGAEIFVKKGQRLLLK
                     PNLLVGEAPEKCVTTHPAVFRAVAEIIKETGAVVSYGDSPAIGSTSAAARKCGIRAEA
                     EDLVIPEADFKTPVEVFFEKGVQNKKFIVAKAVLDNDVVVSLPKLKTHGFEKFTGCVK
                     NQFGCIPGVRKSEYHIKLPNAVQFAQMLVDLNNFVNPALYVMDGVWAMEGNGPRGGTP
                     RKMNILLFSSDPIALDATVCRIIDVDPRHVPTITMGHKSGSGTYLDKEIELVGDDLDS
                     FRLPDFDIMRTPLAPYRDRGIMRLLSNHFVPRPVINAEKCGGCGVCVTICPTEPKSID
                     WVAGDRQKPPTHNYKTCIRCYCCQEICPESSIDIRYPLVRRIFGTSR"
BASE COUNT          618 a          635 c          656 g          535 t
ORIGIN      
        1 acatccgcgc aatctcccct gaacattttc atcgttcgtg ggtgttggac aaacatggca
       61 attcatatga agagggaagt ggcatgaacc gatgcttgtt tttcgtgggc tgcctgctcg
      121 ccggtatcat gggaatgctg ggcggtccgg agccggtcgc ggccgccaag agctacgaat
      181 cgtgcgtgga aatcaacgac gatgccttgc ggctgcgttg ttacgacgag gtcacgggcc
      241 gtaagacaaa acaaccggac gcgcctgttt cagcgcttgc gactcccccc gcgtcacaag
      301 cacccgttgt gacgtccggg agttccgtcc tctcccggat gtggcagctc gacaacgaga
      361 gtcgccggcg caggttcgcc atcatgccac accggcagaa ctacatcctc ccctacacgt
      421 acaatttctc tcaagagaag gagacttatg aaaaagccaa accgggaacg gagctccaga
      481 acgccgaagc caagttccaa atcagcctga agatgaagct ctgggaagat atcttcggaa
      541 caaaaatgga cctgtgggct gcatacaccc aggtctcctt ttggcaggtt tacaacaccg
      601 agcactcctc cccctttcga gagacaaatt acgagccgga actcctcctt acctacccga
      661 caaatataga cctctggggg ctgaaaagtc gttttgttca gattggcatc aaccaccaat
      721 ccaatgggcg tggtgaaccg ctttcacgaa gctggaatcg gttcacggcc aattttggtt
      781 tcgagcggga cgctttcaac ctcatcctca agacctgggt acgtatcccc gagagagaaa
      841 gccaggacga caatcccgat atcacctcct atctcggcta tggggagatc tgggccggaa
      901 ctgtctggaa ggattactcc ctcggactca tgttcaggaa caatctccgt ttcgatgaga
      961 atcgcagcgc cttgcagtgc gaagtgatct tccctttgat cgagtacctc aatgggtaca
     1021 tccagtattt tatcggatat ggagagagtc taatagacta caaccacttt actaaccgaa
     1081 tcggcctggg ggttatggtg aaggactggt agtgacgggt aatgggccat gacaaatcat
     1141 cgccaagaca tttgccctga tgccgcggag acaagggaag ttttaaaaaa tcatattttt
     1201 cgtgaagcct acgaccatag tcgaaaatcc aaagcgatat cggaagtaga catgccagtg
     1261 atgttatgca gtaaaggttg aggaggagga tggtgatgtc gaaagtggca ctgatccgat
     1321 gtgaaagcta tgaatatgga aaggtcagaa gcgctattag gcacggcctc aatctcatcg
     1381 gcggtgcgga aatatttgtc aagaagggac agcgcctcct cctgaagccc aacctgctcg
     1441 ttggcgaagc accggaaaag tgtgtgacca cccaccctgc cgtgttcagg gctgttgccg
     1501 aaatcattaa ggaaaccggt gcggtggtga gctacggcga ttccccagcc attggaagca
     1561 cctccgccgc cgcaagaaaa tgcggtatac gtgccgaggc ggaagatctc gtcattcctg
     1621 aggcggattt caaaacaccc gtggaggtat tttttgagaa gggtgtacag aacaaaaaat
     1681 tcatcgtagc aaaggccgtt ttggacaatg atgtcgtcgt gagtctcccg aaactgaaaa
     1741 cacatggctt cgagaagttc accgggtgcg tgaagaatca attcggttgt attcccggtg
     1801 tgcgcaaaag cgagtaccac ataaagctcc ccaacgccgt acagtttgca cagatgcttg
     1861 tcgacctgaa taatttcgta aatcctgcac tctatgtaat ggacggggta tgggcgatgg
     1921 agggcaacgg gccgcgcggc gggacaccac gtaaaatgaa catcctcctt ttttcaagcg
     1981 acccgattgc cctggatgcg accgtgtgcc gaatcatcga cgtggacccc aggcatgtac
     2041 caacgataac gatgggccac aaaagtggtt ccggaactta tctggacaaa gagattgaat
     2101 tggtgggcga cgatctcgat agtttccgtc ttccggactt cgacattatg cgcacccccc
     2161 tcgcccccta ccgggataga ggcattatgc gcctcttgag taatcacttt gttccccgtc
     2221 ccgtaatcaa tgcggaaaag tgtgggggat gcggcgtgtg cgtgacgatt tgtcccactg
     2281 agcccaaatc gatcgactgg gttgcgggcg atcgacagaa accgcccacg cacaattata
     2341 agacgtgcat tcgatgctat tgctgccagg agatctgccc cgagagctca attgacatcc
     2401 ggtatccgct tgtcaggcgt atcttcggga caagcagatg accc
//
LOCUS       DAOSMF010000225         2407 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_47814_length_2407_cov_18.928997, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000225 DAOSMF010000000
VERSION     DAOSMF010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47814_length_2407_cov_18.928997"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(325..2322)
                     /locus_tag="PLT63_11375"
     CDS             complement(325..2322)
                     /locus_tag="PLT63_11375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOD99363.1"
                     /translation="MNETYRSLVESTSDFFYLVDVRDRYLFANSCYLRWHQLTLDEII
                     AKNYADLHTPEETRIFHEHLCQVISSGNSHQKEYFSAATGRVFLRTYSPARDPAFPDQ
                     INAITIVSKEITKMKTLEQELREKENRYRTLFETANDAIMVGDYYRVLDCNKKTLDLF
                     RCTKDVLMATVPEDLSPLFQPDGALSREKSLALLGKAMEGEPQSFEWRFRRSDGEFFD
                     SEVSMSSMELGGRRCVQILIRDVSELKQAEALYRIIAESSYAGVYMVQDGKFIYLNTN
                     AARYVGFTPEEMVGGAAIDIIHPADRESARRCAVDMLKGLRTAPYEFRVVARDGSHRW
                     IMETLRAVEYRGRRAILANSMDVTELKAMEASLRLSEERYRTIVENMTDGYYEVDLTG
                     KFLFVNDAFAAAAGYRKEEVLGCGFQDFVNEEQAANGRRVFGKVFATGEPVKGFEWQV
                     RDHQGKNLTLEVSIILIRDAENRPRGFRGIARDITERRRAEKQIRYYAFHDVLTGLPN
                     RNLLYDRVEIALANAARKNRGLAVMLLDLDNFKKLNDTMGHAAGDLILKGVAKRLQHL
                     VRKGDTVARIGGDEFIMVLTDVRYPGDAVNIAEKVIAAFREPFFIDGLEVRLTVSAGV
                     SLYPRDGMDFDRLKRSADNAMYKAKDKGRNCYVLTEAARPS"
BASE COUNT          492 a          698 c          692 g          525 t
ORIGIN      
        1 cttaacaaaa aatgaccgtc aaggcaaata gattgcacat ttttctccgc ccggccggaa
       61 attcgtgaca gcgggaaaga taccccggaa ccgggtcgcc aagacggatt acatggtcct
      121 cagggagatg gcctaaagtg ggaggaagaa aactgccaac cgggggacaa gctactgctc
      181 ataaatggat tcccgccttg tctttcccgg gagggagaca aataggatca ttgtccccct
      241 caatccgaca aaaaatgtgg ccccggtacg gcggttgtta cctgagtcga tctccgttta
      301 cattggccga atgccgggta aagactacga agggcgcgcg gcctcggtca agacgtagca
      361 gttgcgccct ttatccttgg ccttgtacat ggcgttgtcg gcggatctct tcagacgatc
      421 gaaatccata ccgtcccggg gataaaggga gacgccggcg ctcaccgtga ggcgcacctc
      481 caggccgtcg atgaaaaacg gttctctgaa ggcggcaatg accttttcgg cgatattgac
      541 agcatcgccg ggataacgga cgtccgtcag gaccatgatg aattcatcgc cgccgatgcg
      601 cgccacggtg tctcccttgc ggacgaggtg ttgcaggcgc ttggcgacgc ccttgaggat
      661 caaatccccc gccgcgtgtc ccatggtgtc gttgagcttt ttgaagttgt ccagatccag
      721 aagcatcacg gccagtcccc gatttttccg ggcggcgttg gccagggcga tttcgacccg
      781 gtcgtacaga agattgcggt tcggaagtcc cgtgagaaca tcgtggaagg catagtaacg
      841 gatctgtttc tccgcccggc gccgttccgt gatgtcccgg gcgatgcccc gaaacccgcg
      901 gggacggttc tcggcgtcac gaatcaggat tatggacacc tccagggtca gattcttgcc
      961 ctggtggtcg cggacctgcc attcaaatcc cttcaccggt tcgccggtgg cgaatacctt
     1021 tccaaagacg cgccgcccgt tcgcggcctg ttcctcattc acgaaatcct gaaagccgca
     1081 accgaggact tcctccttgc ggtatcccgc ggcggcggca aaggcgtcgt tgacaaagag
     1141 gaatttgccc gtcaggtcca cctcgtagta cccgtccgtc atgttctcca cgatcgtccg
     1201 gtagcgttct tcgctgaggc gcagcgaggc ctccatcgcc tttagttccg ttacatccat
     1261 ggaattggcg aggatcgccc gcctcccccg gtactccacg gcccggagcg tttccatgat
     1321 ccaccggtgg ctgccgtccc tggccaccac ccgaaattcg tagggggcgg tccgcaggcc
     1381 cttgagcata tcgacggcgc atctccgcgc cgattcccgg tctgcgggat ggatgatgtc
     1441 gatggccgcc ccgccgacca tttcctcggg ggtaaagccc acgtaacggg ccgcattggt
     1501 gttgagatag atgaacttcc catcctgaac catgtacacc ccggcatagg aactttccgc
     1561 gatgatccgg tagagggcct ccgcctgttt caactccgac acgtcccgga tcaggatctg
     1621 aacgcagcgc cggccgccga gttccatgga actcatggac acctcgctgt cgaagaactc
     1681 accgtccgag cggcggaagc gccattcaaa ggattggggt tcgccttcca tcgccttgcc
     1741 cagcagtgcc agcgatttct ccctggacaa ggccccgtcg ggctggaata acggggaaag
     1801 atcttccgga acggtggcca taagaacatc cttggtgcac cggaataaat cgagggtctt
     1861 tttattgcag tccaggacgc ggtagtaatc acccaccatg atggcgtcgt tggccgtctc
     1921 gaagagggtg cgataacgat tttccttttc tcggagttcc tgctccaggg tcttcatctt
     1981 ggtgatttcc ttggagacga tggtgatggc gtttatctgg tcgggaaagg cggggtccct
     2041 ggcggggctg taggtgcgga gaaatacccg gccggtggcc gcgctgaaat actccttctg
     2101 atgggaattt ccgctggaaa taacctggca tagatgttcg tgaaagatcc gcgtctcttc
     2161 cggggtatga agatccgcgt aattcttggc gatgatttcg tcgagcgtaa gctgatgcca
     2221 gcggagatag caactattgg cgaagaggta ccggtcgcgt acatccacca gataaaagaa
     2281 atcgctggtc gattccacca gggaacgata cgtctcgttc attcacccgt gggcaaaggc
     2341 agtttccccg attgtgccga tggtttcttt cagatcctga aacgtaggtt ccatgtcccc
     2401 ctcatac
//
LOCUS       DAOSMF010000226         2385 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_48345_length_2385_cov_18.945923, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000226 DAOSMF010000000
VERSION     DAOSMF010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48345_length_2385_cov_18.945923"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="PLT63_11380"
     CDS             complement(<1..572)
                     /locus_tag="PLT63_11380"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOD99364.1"
                     /translation="MPDRGKEELLLLIGALRAHVEAEASAGRNTISFRIGGDGVMAID
                     KALLPASGEIFVAASPSAVPVETVPLRATGAPCPELMGAAEPERITTPLASPNVPLDL
                     KALREELGDCRRCRLHLGRRNIVFGEGSPRAALAFVGEAPGADEDAQGRPFVGRAGQL
                     LTRIIEAMKMKRSEVYICNILKCRPPGNRNP"
     gene            complement(556..1755)
                     /gene="coaBC"
                     /locus_tag="PLT63_11385"
     CDS             complement(556..1755)
                     /gene="coaBC"
                     /locus_tag="PLT63_11385"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HOD99365.1"
                     /translation="MAAGKKAVLGVTGGIAAYKAAELARLLVKKDISVMVVMTENAKE
                     FITPLTFQTLTGHPAFSDLFSQEHYDLNHISLAAYGDVIVVAPATANIIGKMAAGIAD
                     DLLSTVLLAARAPVLVCPAMNDNMYTHPAVQNNIRILQNRGVHIVAPDRGELACKTAG
                     IGRLAELEAIVEAIETAWTDKDLAGEKILVTAGPTREPFDPVRFITNYSSGKMGYALA
                     TMARRRGAEVILVSGPTVLPIPYGVDFIPVESAVEMRGAVMSRLETVTVVIKAAAVAD
                     YRPAVRAGNKIKKKRGPLNIDLEKNPDIIAEIGAKKGKRVVVGFAMESENLLANAKAK
                     LFAKNMDLIVANDLHQDGAGFQSDTNIIKILDLQGGVETVPLMDKLDIAARILDRVKA
                     LIYAGSR"
     gene            1957..>2385
                     /locus_tag="PLT63_11390"
     CDS             1957..>2385
                     /locus_tag="PLT63_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99366.1"
                     /translation="MKTYKIYFITGAVIVISALAWWFFATIGEFGAPRIDLMQDLRGI
                     GPRTVLEITFSDTGSGLQETAVTLTQDNQPRVLSKLEFSGPKTKRHVVSIPIDPQALK
                     LHDGPATLTVTAADHALWKNSTVVNRLVNVDVIPPQIFLLT"
BASE COUNT          476 a          730 c          651 g          528 t
ORIGIN      
        1 ggattgcggt ttcccggcgg ccggcatttg aggatattgc agatgtaaac ctccgaacgc
       61 ttcatcttca tggcctcgat aatcctcgta aggagctgcc ccgcccggcc tacgaacggc
      121 ctcccctggg cgtcttcatc ggccccgggc gcttcgccga caaatgccag ggccgcccgg
      181 gggctgccct ctccgaagac gatgttcctt cgtccaagat ggagccgaca ccgccggcag
      241 tctcccagtt cctcgcgcaa cgctttcaga tccaggggaa cattcggaga cgccaagggg
      301 gtagtaatcc gctccggttc cgccgccccc attagctccg ggcaaggcgc cccggttgcc
      361 cgtagcggca ccgtctccac ggggacggct gatggggatg ccgccacaaa tatttctccg
      421 gacgcgggga gcagcgcttt atcgatcgcc attacgccgt ctccaccgat acggaatgag
      481 attgtattcc ggccggcgga cgcttccgcc tctacatggg cccgcagcgc cccgatcaac
      541 aggaggagtt cttctttacc tcgatccggc atagataagc gccttgacgc gatccagtat
      601 ccgggccgcg atgtctagct tgtccatgag ggggaccgtt tcgaccccgc cctggagatc
      661 gagaatcttg atgatgttgg tgtcggattg gaaacccgct ccgtcctggt gaagatcgtt
      721 ggccacgatc agatccatat tcttggcgaa gagttttgcc tttgcgttgg ctaggagatt
      781 ttccgattcc atggcaaaac ccacgactac gcgctttcct ttttttgctc cgatttcggc
      841 gatgatatcg ggattttttt ccagatcaat attcaaggga ccacgtttct tttttatttt
      901 gttgccggcg cgcacggccg gacgataatc ggcgacagcc gccgccttga tgaccaccgt
      961 gacggtttcg aggcggctca tgacggcgcc gcgcatctcc acggcgctct ccacggggat
     1021 gaaatcgacc ccatagggga tgggcaggac cgtgggaccg ctgacgagga tcacctccgc
     1081 cccgcgccgt cgggccatgg tcgccagggc atagcccatc tttcccgatg aatagttggt
     1141 aatgaagcgg acgggatcga atggctcccg ggtgggaccg gcggtgacga gaatcttttc
     1201 cccagccaga tccttatccg tccaagctgt ctcgatagcc tcgacaatcg cttccagttc
     1261 ggccagtctt ccgatcccgg cggtcttgca ggccagttcg ccacgatctg gcgccacgat
     1321 atgaacacct cggttctgaa gaatcctgat attgttctgt acagccggat gggtgtacat
     1381 gttgtcgttc atggccgggc agacaagcac tggcgcccgg gcagccagga ggaccgtgga
     1441 aagcaaatcg tcggcgatgc ccgccgccat cttcccgatg atattggcgg tggccggcgc
     1501 tactacgatc acatccccat aagccgccaa agagatatgg ttcagatcgt aatgctcctg
     1561 ggagaataga tcgctgaagg caggatggcc cgtaagggtt tgaaaggtca gcggcgtgat
     1621 gaattccttg gcattttccg tcatcaccac catgacggaa atatcctttt tcaccagcaa
     1681 tcttgccagt tcagccgcct tgtacgcggc gatgcctccg gtgacgccga ggaccgcctt
     1741 tttcccagcc gccatttttt ctgtccctcc cgattggcca ctcattttgc ggcaactata
     1801 gcaataatga acgccaagat caaggcaagt aatgcaccgt ttgcgcttca tctatgactt
     1861 gcaaaaggag gggaaaatca ttatagagcg gactgtgttt ttccgcgttt ccacttccac
     1921 ggcggcaacg aaaaaatctc gacgtacagg aaggccatga aaacctacaa gatctatttc
     1981 ataaccggag cggttatcgt cataagtgcc ctggcctggt ggtttttcgc caccatcggt
     2041 gaattcggcg ccccccggat cgacttgatg caggaccttc gcggcatcgg cccgcgcacg
     2101 gtgctggaaa tcaccttttc cgataccggt agcggtctcc aggaaaccgc cgtcaccctc
     2161 acccaggata accagccccg ggtgctgtcg aaactggaat tttccgggcc caagacaaaa
     2221 cgccacgtcg tttcgatccc catcgatccc caggcgctca agctccatga cggcccggcc
     2281 accctgactg taacggcggc ggatcatgcc ctatggaaga attccactgt cgtcaaccgc
     2341 ctggtgaatg tcgatgtcat cccgccgcag atctttttgc tcacc
//
LOCUS       DAOSMF010000227         2364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_48911_length_2364_cov_19.654829, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000227 DAOSMF010000000
VERSION     DAOSMF010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48911_length_2364_cov_19.654829"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="PLT63_11395"
     CDS             <1..604
                     /locus_tag="PLT63_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418923.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HOD99367.1"
                     /translation="ALAQPDVIGLSIGTRPDCIPDPVLDLLEGYTRQAHIWLELGLQS
                     IHDRTLADLNRGHDARTFLDAVARVQGRGIHLCVHIIVGLPGENRADILATARVLAGL
                     PVQGIKIHSLLALRGTPLGRRYENGDVFLITKEEYVTTVCDILEILPPATVIQRLTAD
                     GYKDIFLGPSWAVNKMDALNSIDWELKRRRSWQGSRRDSL"
     gene            611..1093
                     /locus_tag="PLT63_11400"
     CDS             611..1093
                     /locus_tag="PLT63_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99368.1"
                     /translation="MTRDAVRWCRRAAVFHKNDQADQHRSQCGEQDRRGGDVFGSADE
                     GMVIGMDGIGQELNGRVHSLSYPDRHDGNDHQSPFPALECKPNARGNDGNRGHEMNAG
                     MRLGCEQIARTSQGASEAADTPIKIGHSRGRSPLFRVGHKIIVTSCISASIANSAPKS
                     "
     gene            complement(1211..2131)
                     /locus_tag="PLT63_11405"
     CDS             complement(1211..2131)
                     /locus_tag="PLT63_11405"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOD99369.1"
                     /translation="MPMIISRYRNGSGAGRRWVYLISMVWLALIPLFPFVGAAAESSA
                     GPSLTSNRPAVPPDGNAGGKGMELDRDYLKGYVRDTGKILSSPVRWEGKDWLTASLVV
                     AATAGFYAYDQDIKEWFQDKRSDASNNVAKVITPLGNPLFAVPAMGLVYLYGEHQESE
                     KARRIGLLGVESIVLAGAFTSVLKFVTHRPRPDAGERYDKWHGPEFSTDNLSFPSLHT
                     STAFSMAKVIASETDNPCVAAAVYSLASLVGLARLNDNEHWASDAFFGAAIGYFTAAA
                     VLRYHKRSSPVSFMPSVSRNGGGISLTYRY"
     gene            complement(2144..>2364)
                     /locus_tag="PLT63_11410"
     CDS             complement(2144..>2364)
                     /locus_tag="PLT63_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99370.1"
                     /translation="VHFASGGWGGAEGCVTLAVEGPDAKVNKCLKFIEQKIKGEPPLP
                     ALKSPCKTCGIVCNFKGKDPRQLPAWLK"
BASE COUNT          495 a          749 c          671 g          449 t
ORIGIN      
        1 ggccctcgcc cagcccgatg tgatcggtct gtccatcggc acccgtcccg attgcattcc
       61 cgatcctgtg ctcgacctac tggaaggcta taccaggcaa gcccatatct ggctggaact
      121 aggtctccag tccatccacg atcggaccct cgccgacctg aacaggggcc acgacgcccg
      181 aactttcctc gacgcagttg cgcgggtcca gggccggggg atccatcttt gtgtccatat
      241 catcgtcggg cttcccggag aaaaccgggc ggacatcctg gcgaccgccc gcgtcctggc
      301 cggcctgccc gtccagggca tcaagatcca ctccctgttg gcgctccggg gcacgcccct
      361 gggccgtcgt tacgagaacg gtgacgtgtt cctcataacc aaggaggaat acgtcaccac
      421 cgtctgcgac atcctggaaa tcctgccgcc cgcaacggtg atccagcgcc tcaccgccga
      481 cggctacaag gacatcttcc tcggtccatc ctgggcagta aataaaatgg acgccctcaa
      541 cagcatcgac tgggagctca agcgccgccg gagctggcag ggcagcaggc gggatagtct
      601 ttgagaagca atgacgcgtg acgcggtgcg ctggtgccgc cgggcagcag tatttcacaa
      661 aaacgaccag gccgatcaac accgctccca gtgcggcgaa caggaccgcc gcggcggcga
      721 tgtctttggc tctgccgatg aggggatggt gatcgggatg gacggcatcg gccaggaact
      781 caatggccgt gttcattccc tcagctatcc agaccgccat gatggcaacg atcaccagtc
      841 cccattccca gccctcgagt gcaaaccaaa tgcccgcggc aacgacggca accgaggcca
      901 cgagatgaat gcgggcatga ggctgggatg cgagcagatc gcccgcacct cgcagggcgc
      961 aagcgaagct gcggatacgc cgatcaagat aggacatagc cgtggtcggt cccccttatt
     1021 tagggtcggt cataaaataa ttgtaacatc ttgcatctcg gcttcaatcg caaattcagc
     1081 tccaaagagc tgaatcatgt tcataaatcc tcaacgtagc gctgcttcgc ctgcggtttg
     1141 gcggaatcag tcatgacaaa tacagcctca atctgagcgc cactttgcta ccttttttca
     1201 tgacagactc ttagtagcgg taggtaagcg aaatcccgcc gccattgcgg ctcacgctgg
     1261 gcataaatga aacggggctc gaccgtttat ggtagcgaag gacggcagcg gcggtaaagt
     1321 aaccgatggc cgccccaaaa aaggcatccg acgcccagtg ttcattgtcg ttcagccggg
     1381 ccagccccac cagcgaggcc aggctgtaaa ccgccgccgc cacacaggga ttgtctgttt
     1441 cggaggcgat gaccttcgcc atggaaaagg ccgtactggt atgcagtgat gggaaagaca
     1501 gattgtccgt cgagaattct ggtccatgcc acttatcgta acgctccccg gcgtccggac
     1561 ggggccggtg cgtgacaaat ttcaggaccg aggtgaaagc cccggccagg acgatgctct
     1621 ctacccccaa caggcctatc cgcctcgcct tttcgctctc ctgatgttcc ccgtagaggt
     1681 aaacgagtcc catggccggc acggcaaaca acgggttgcc taggggggtg atcaccttgg
     1741 cgacattgtt ggaggcgtcg ctcctcttat cttgaaacca ctctttgata tcctgatcgt
     1801 aggcataaaa tcccgccgta gccgcgacaa ccagggaggc cgtcagccag tcctttcctt
     1861 cccagcgcac cggagagctg aggatcttac ccgtatcgcg tacatatcct ttcagataat
     1921 cgcgatccaa ttccatcccc ttcccaccgg cattcccgtc cgggggaacg gcgggccggt
     1981 tggacgtcag ggatgggccg gcagacgact ccgcggccgc gccgacgaaa gggaaaagag
     2041 gaatcagcgc cagccatacc atcgagatca ggtaaaccca tctccgcccc gcgccgctac
     2101 cgttccggta cctcgagatt atcatgggca tgggccgcaa atcctacttt agccaggccg
     2161 gcaactgccg tggatccttg cccttgaagt tgcacactat cccgcaggtc ttgcaggggc
     2221 ttttgagggc cggaagcggc ggttcgccct tgatcttctg ttcgatgaac ttgaggcact
     2281 tgttgacctt ggcgtccggt ccttccacgg ccagggtcac gcagccttcc gcgccgcccc
     2341 agccaccgga ggcgaagtgg acgg
//
LOCUS       DAOSMF010000228         2302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_50670_length_2302_cov_18.309746, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000228 DAOSMF010000000
VERSION     DAOSMF010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50670_length_2302_cov_18.309746"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="PLT63_11415"
     CDS             <1..277
                     /locus_tag="PLT63_11415"
                     /inference="COORDINATES: protein motif:HMM:TIGR04169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arsenosugar biosynthesis-associated
                     peroxidase-like protein"
                     /protein_id="HOD99371.1"
                     /translation="APTLGEKYFAYYGEAMQDGVLTAREKALIALAVATTQKCPYCID
                     AYTNQCLSLGVAPEEMMEAVHVGAAMTAGVVLAHSTQMRKIIKKKEM"
     gene            280..1242
                     /gene="arsS"
                     /locus_tag="PLT63_11420"
     CDS             280..1242
                     /gene="arsS"
                     /locus_tag="PLT63_11420"
                     /inference="COORDINATES: protein motif:HMM:TIGR04167.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM protein
                     ArsS"
                     /protein_id="HOD99372.1"
                     /translation="MNGQGKHPPFAERAAGPVLATGIEIFQINLGYRCNLECRHCHVA
                     AGPERRELMSRELMERCLDILRAHPIPTIDITGGAPELHPHFPWFLEECAALGRRLLV
                     RTNGVVLLEEGYGSFLDLYTRLRVEVVVSLPHVDPRITDRQRGEGVHARLIAVLRKLN
                     ARGYGQPGSGLVLNLVHNPGGAYLPGLQGSLEARYRQVLRDKYGIGFNNLFCITNMPI
                     GRYLAFLKRSGNYEDYMDALVRAFNPLALANVMCRTTLSVGWDGRLYDCDFNQMLGLT
                     VNHGAPEHIERFDGKMLATRRVVTGNHCYGCTAGAGSSCQGEVA"
     gene            1242..2033
                     /locus_tag="PLT63_11425"
     CDS             1242..2033
                     /locus_tag="PLT63_11425"
                     /inference="COORDINATES: protein motif:HMM:NF022694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HOD99373.1"
                     /translation="MKGRQEGLRPRTGRWPRRRDGREITTFLPLLLFWAVVMPLLAPV
                     SSPAADPVVLFREDFASLARWEPLTFPKIKAHSTYTLVREGEKSVLKAESRASASGLV
                     CRQTFNIYEYPRLRWRWKVTNLTDRGDPREKEGDDYPIRIYVMFQYDPARATTGERLV
                     YNATRLIYGKYPPHSTLNYVWTGRHIPERFIVSPYTDKARMVVLERGKERLGRWVEES
                     VNVLDDYRRAFGKEPPALAGIAVMSDTDNTGATAEAYVDFIEIRR"
     gene            2068..>2302
                     /locus_tag="PLT63_11430"
     CDS             2068..>2302
                     /locus_tag="PLT63_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HOD99374.1"
                     /translation="MSDYRKMWADLGLDLESHDALLSVLGGAYGDIYLAQKNRPEGMK
                     YFDFVMSEVHGLRIKELMDAKAQGRKVIGSYCVF"
BASE COUNT          444 a          753 c          695 g          410 t
ORIGIN      
        1 cgccccgacc ctgggggaga agtatttcgc ctactacggc gaggcgatgc aggacggcgt
       61 cctgacggcg cgggaaaagg ccctcatcgc cctggccgtg gccactaccc agaagtgccc
      121 ctactgcatc gacgcctata ccaaccagtg cctgtccctg ggcgtcgctc cggaggagat
      181 gatggaggcc gtccacgtgg gagcggccat gaccgccggc gtcgttctgg cccactccac
      241 ccagatgcgg aagatcatca agaaaaagga gatgtagcca tgaacggaca gggtaagcat
      301 ccccctttcg cggaacgggc ggccggtccc gtcctcgcta cggggatcga gatcttccag
      361 atcaacctcg gctaccgctg caacctggag tgccgccact gtcatgtcgc cgcgggtccg
      421 gaacggcggg aactcatgtc ccgggagctg atggagagat gtctggatat cctccgcgcc
      481 catccgatcc ccaccatcga catcaccggc ggcgccccgg aactccatcc ccattttccc
      541 tggttcctgg aggaatgcgc cgccctgggc cgtcgtctcc ttgtccggac caacggggta
      601 gtactcctgg aagagggata cggatcattt ctcgatcttt acacccgcct gagggtggag
      661 gttgtcgttt ccctccctca tgtggatccg aggattacgg accgccagcg gggcgaaggc
      721 gtccacgccc gcctgatcgc cgtcctccgc aagctcaacg cccggggcta cgggcagccc
      781 ggcagcggcc tcgtcctgaa cctggtccac aaccccggcg gcgcctatct tccgggtctc
      841 cagggcagcc tggaggcgag gtaccgccag gtcctccggg acaaatacgg gatcggcttc
      901 aacaacctct tctgcatcac caacatgccc atcggccgct atctcgcctt cttgaaacgg
      961 tcgggcaact acgaggacta catggacgcc ctggtgcggg ccttcaatcc tctcgccctg
     1021 gcgaacgtca tgtgccggac caccctttcc gtcggctggg acggccgact ctacgactgc
     1081 gatttcaacc agatgctcgg cctgacggta aatcatggcg ccccggaaca tatcgagcgc
     1141 tttgacggaa aaatgctcgc cacgcgccgc gtcgtcacgg ggaaccactg ttacggctgc
     1201 accgccgggg cgggaagctc ctgccagggc gaggtggcct aatgaaggga aggcaagaag
     1261 gtcttcgccc ccggacaggt cgatggcccc gccgccggga cggccgggag ataaccacct
     1321 tcctcccgct gctcctcttc tgggccgtag tcatgcccct cctcgctccc gtttcgtctc
     1381 ctgccgcgga cccggtggtc ctcttccggg aggacttcgc cagcctcgcc cggtgggaac
     1441 ccctgacctt ccccaagatc aaggcccatt ccacctatac cctggtcaga gagggggaga
     1501 agtccgtcct caaggcggaa agccgcgctt ccgcctcggg cctcgtctgc cgtcaaacct
     1561 tcaatatcta cgaatatccc cggctccgct ggcgctggaa ggtgaccaac ctgacggacc
     1621 ggggcgatcc ccgggaaaag gaaggggacg actaccccat ccggatctat gtaatgttcc
     1681 agtacgatcc ggcccgggcg acaacggggg agcggctggt ctataacgcc acccggctga
     1741 tctacggcaa atatcccccc cacagcacct tgaattacgt ctggacggga cggcacattc
     1801 ccgagcgctt catcgtcagc ccctatacgg ataaggcccg gatggtcgtc ctggagcggg
     1861 ggaaggagcg tctcggccgg tgggtggaag agtcggtcaa tgtcctggac gactaccgcc
     1921 gggccttcgg caaggaacct ccggccctgg ccgggatcgc cgtcatgagc gatacggaca
     1981 acaccggcgc caccgccgag gcgtacgtcg attttatcga aatcagacgt tgagaagttg
     2041 aactataaaa aaataaggag gttatgcatg tcagattaca gaaagatgtg ggcggatctc
     2101 gggctcgacc tggaaagcca tgacgcgctg ctgtccgtgc ttggcggggc ctacggggat
     2161 atctacctgg cccagaagaa tcgccccgag gggatgaaat atttcgattt cgtcatgagc
     2221 gaggtccacg gcctgcggat caaggagctg atggacgcca aggcccaggg gagaaaggtc
     2281 atcggctcct attgcgtctt cg
//
LOCUS       DAOSMF010000229         2288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_51046_length_2288_cov_20.378415, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000229 DAOSMF010000000
VERSION     DAOSMF010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51046_length_2288_cov_20.378415"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(326..1702)
                     /locus_tag="PLT63_11435"
     CDS             complement(326..1702)
                     /locus_tag="PLT63_11435"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOD99375.1"
                     /translation="MTARTSFPASGGNRLPGYSTPDAGALLHGHKEKIPFPVSPVIHS
                     ALRAIYAGGPKLKWQADRNGRPFYSYVTKAGDITFFCEPPPDYSRRFHPTIAMYYTPR
                     LGFIYAGSLRDKVRNLSVETADVLLILMSYVARLADPRTGIARITLEEMAAMRDVHLR
                     RGRSQNLYAALKEEVLRIADLRLSMTWKDYRTGGGVAFGKERPDRLLDILDVEYEQQD
                     RPGTAFRFRCGQALSHFLDAAGLFWIGYYSRSLLHLSPYHEVFTKKVGTYWTLIGTIA
                     GKKGGLPRATPRTILDFCGDDVHGRNPGYTVDAFFKAHERLREIGVIADDDLREPVSR
                     TRGYMQAWLEDPITVRLSERLWKPAPRFHERKPSRSDRRFPFRSQEFRDREEVPRDVA
                     ALWADPMVLKRLRARFYLRQEDLSRAVGISRQTLSRYERGICGIPPACADKILTFLHK
                     KVGRDWEQ"
     gene            complement(1906..>2288)
                     /locus_tag="PLT63_11440"
     CDS             complement(1906..>2288)
                     /locus_tag="PLT63_11440"
                     /inference="COORDINATES: protein motif:HMM:NF020926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HOD99376.1"
                     /translation="DRAIGDKSGEWFVKKGKELREITNFTGAAAAGPAPGNGTAGFAG
                     PPGGSEAARLDAYDPTIFIFDAYPGGIGFSELLYGQHEDLLAAVRHLLRSCPCTHGCP
                     SCVGPTLEVGPTAKEVALAILDRL"
BASE COUNT          420 a          648 c          754 g          466 t
ORIGIN      
        1 cgggctgggc ggtgccggta tagtaaagga tgttgcggga ataggcaatc agggcacctc
       61 cccatccctg gacggtgagt tgccgctgca ggaccgtaat tcttgtcgtt gccttcatga
      121 ttgttttcct ccctgttggc gagatccggc ggtcgatttt gggagcgatg ataacaccgt
      181 gccgttttca gcctgccggc ggttaatttt tattagtgaa gttaacaggc ggacaagata
      241 cgtgaatatt agctcgccgg cgctgttgcc ggtgcattct taggggatgg gtgcctcaaa
      301 aacgggggat aggtaagtcc gcaggttatt gttcccagtc ccgtccgact tttttatgca
      361 aaaaagtcaa tatcttgtcc gcgcaggcgg ggggaatccc gcatatcccc cgttcgtagc
      421 gcgacagggt ttggcgggag atcccgacgg cccgggacag gtcttcctgg cgaagataga
      481 agcgggcgcg tagccttttc aagaccatag gatcggccca cagggcggcg acgtcccggg
      541 gaacctcctc gcgatcccgg aactcctgcg agcggaatgg gaaccgacgg tctgatctgg
      601 acggcttcct ttcgtgaaac cgcggcgccg gtttccacaa tcgctcggaa agccggacgg
      661 taatcgggtc ttccagccac gcctgcatgt atccccgtgt ccggcttacg ggttcccgca
      721 gatcatcgtc ggcaatgacc ccgatttccc gcagacgttc gtgggccttg aagaaggcat
      781 cgacggtgta gccgggattg cgcccatgca cgtcatcacc gcagaagtcg aggatcgttc
      841 ggggggtggc gcggggcagt ccgccttttt tcccggcaat ggtgccgatg agggtccagt
      901 atgtccctac ctttttggta aagacttcat gataagggct cagatggaga agggaacggc
      961 tgtaatagcc gatccagaac aaacccgcgg cgtcgaggaa atgcgacagg gcctgcccgc
     1021 agcggaagcg gaaggccgtc ccgggacggt cctgctgctc atattccacg tccaggatat
     1081 cgagcagccg gtccggccgt tctttgccga aggccacgcc gccgccggtc cggtaatcct
     1141 tccaggtcat ggacaggcgc aggtcggcaa tgcgcagcac ctcttccttg agagcggcgt
     1201 ataggttttg cgagcgcccc cggcggaggt ggacatcccg catcgccgcc atttcttcga
     1261 gggtgatccg ggcgatcccg gttcgcggat cggcgagccg ggcgacgtag gacatgagga
     1321 tgagaagcac gtcggcggtc tccacggaga ggttccgcac cttgtcccgc aggctgccgg
     1381 catagatgaa gccgaggcgc ggggtgtaat acatggcgat ggtcggatga aaacggcggg
     1441 aataatcggg tggtggctcg cagaaaaagg tgatgtcccc cgctttggtg acatatgaat
     1501 agaagggacg gccgttgcgg tccgcctgcc atttcagttt cgggccgccg gcataaatgg
     1561 cccgcagggc ggagtggatg accggactga cgggaaaagg gatcttttcc ttgtggccgt
     1621 gaagcagggc accggcgtcc ggggtggaat atcccggaag acggttcccg ccagatgccg
     1681 gaaatgatgt cctggccgtc atgccctgcc cccctgcctt ttgcccggct tgcggtcccc
     1741 gctgctccgg atggagttcg gtgccgcctg ccgctgtcgc gatggcgtca gatgaccttc
     1801 atcgccgtgt tttcttactt tttcatcacc ggagattaat aatttgcatg ttagggcgat
     1861 tgtcggggga agtcaacgag gaaaaaagag attttgggcg cggacctaca accgatccag
     1921 gatggccagg gccacctcct tggcggtagg gcccacctcc agggtcggac cgacgcagga
     1981 ggggcagccg tgggtgcagg ggcagctccg gaggaggtgg cggacggcgg cgagcaggtc
     2041 ttcgtgctgg ccgtaaagga gctcggaaaa gcctatcccc cccgggtagg catcgaagat
     2101 aaagatcgtc gggtcgtagg cgtcgaggcg cgctgcttcg ctgccgccgg gggggccggc
     2161 gaagcccgcc gtgccgtttc ctggtgccgg tccggccgcc gccgcacccg tgaaattcgt
     2221 aatctcccgt aattccttgc cttttttcac gaaccactcc ccgctcttgt cgccgatggc
     2281 ccggtcga
//
LOCUS       DAOSMF010000230         2244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_52383_length_2244_cov_19.250799, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000230 DAOSMF010000000
VERSION     DAOSMF010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52383_length_2244_cov_19.250799"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /gene="folP"
                     /locus_tag="PLT63_11445"
     CDS             <1..697
                     /gene="folP"
                     /locus_tag="PLT63_11445"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOD99377.1"
                     /translation="AEGADVIDIGGESSRPGALPVSPEEEMNRVLPVIAAVAGRTDIP
                     ISIDTTKAKVAGRAIAAGAEIINDISALRFDEQMPAVARDSGAAVILMHMRRTPRDMQ
                     TGDLSYRSLWGEIVSFLAGRIEDARDRGIPEERIVIDPGLGFGKTATDNLRLIRELKE
                     CRVLGRPILMGPSRKSFLGRITGETLENLREETVAAVTACILNGARMVRVHEVRAAKK
                     AAAVADAIRKGRS"
     gene            694..1077
                     /gene="acpS"
                     /locus_tag="PLT63_11450"
     CDS             694..1077
                     /gene="acpS"
                     /locus_tag="PLT63_11450"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOD99378.1"
                     /translation="MIAGIGIDLVEVPRMQKILRRWGDRFTARVYTPEEIAYCRGKAL
                     PAIHFAARFAAKEAALKALGLGLGMGLDLREVGVARNNAGLPRLAFTERGLRMLGERA
                     ICRTHLSMTHTRELALAMVIMETQT"
     gene            1080..1553
                     /gene="tsaE"
                     /locus_tag="PLT63_11455"
     CDS             1080..1553
                     /gene="tsaE"
                     /locus_tag="PLT63_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOD99379.1"
                     /translation="MKIVIASKSSEETLRIGRIIGKTASPGEVIALVGELGTGKTCLT
                     QGIARGLAVPEAYPITSPTFTLINEYPGKLDLYHFDIYRLEGVKDLEDMGYEDYFYGE
                     GVTVIEWAERSMPLLPAGTLFVYLAYRGENDRELILTGNNELIGKINNLCKEGGC"
     gene            1557..>2244
                     /locus_tag="PLT63_11460"
     CDS             1557..>2244
                     /locus_tag="PLT63_11460"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOD99380.1"
                     /translation="MALVVQKYGGTSVADIKRIENVARRVIKTKQAGNDVIVVLSAMA
                     GETDRLINLAHQAAADPDSREYDALISTGEQVTVTLLSMVLNRLGYKARSFLSFQIKI
                     NTDRAHKKARITKVDTRKMREELKKGSVLVVAGFQGVDEENDITTLGRGGSDTSAVAL
                     AAALKADLCEIYTDVDGVYTTDPNIYDKARRLERISYDEMLEMARAGAKVLQPRSVEM
                     AKKYEVPIYVK"
BASE COUNT          522 a          557 c          743 g          422 t
ORIGIN      
        1 ggcggaaggc gccgatgtga tcgacatcgg tggggaatcg tcgcgtcccg gcgccttgcc
       61 cgtgtcgccg gaggaggaaa tgaaccgggt gctcccggtg attgccgccg tcgcgggacg
      121 gaccgatatt ccaatctcca tcgacacgac caaggcgaag gtggccggga gagcgatagc
      181 agccggggca gagatcatca acgatatcag tgcgctgagg tttgacgaac agatgcccgc
      241 ggttgccagg gacagcggcg ccgccgtcat cctcatgcac atgcggagga cgccccggga
      301 catgcaaacg ggggatcttt cctatcggtc gctgtggggg gagatagtca gttttctagc
      361 tgggcggatt gaggatgccc gggacagagg catcccggag gagcggatcg tcatcgatcc
      421 cggtttggga ttcggaaaaa ccgccacgga caatctgagg ttgatcaggg agcttaagga
      481 atgccgcgtc ctcggccggc cgattctcat gggtccttcc cgcaaatcgt ttctgggccg
      541 gatcaccggg gaaacgctgg agaacctgag ggaagagacc gtggcggcgg taacggcgtg
      601 catcctcaac ggcgcccgga tggttagggt ccacgaggtg cgggcggcga aaaaggccgc
      661 ggccgtcgcc gatgcgataa ggaagggaag atcgtgatcg ccggcatcgg catcgacctg
      721 gtggaggtcc cacggatgca aaagattctg cggcgctggg gcgacaggtt caccgctagg
      781 gtttataccc cggaggaaat agcctattgc cggggaaaag ctctgccggc aatacatttt
      841 gccgcccggt ttgccgccaa ggaggcggcg ctgaaggccc tgggcctcgg attgggtatg
      901 ggtctggacc tgcgggaggt gggagtggcg cgaaacaatg ccggccttcc tcggcttgct
      961 tttaccgaac gcggcctccg gatgctcggg gaaagggcaa tttgccggac gcatctaagt
     1021 atgacccaca cccgggaatt ggcgctggca atggtcatca tggagacgca gacctgaaaa
     1081 tgaaaattgt gatcgcctcg aagagttctg aagagaccct gaggatcggc aggatcatcg
     1141 ggaagacagc gtcacccggg gaggtcatcg ccctggtagg tgaactgggg accgggaaga
     1201 cctgcctcac ccaggggatc gcccggggac tggccgtacc cgaggcatat cccataacca
     1261 gccccacctt tacccttatc aatgaatatc ctggcaagtt ggacctttat cattttgata
     1321 tctaccgtct cgaaggggtg aaggatctgg aagatatggg ttatgaagat tatttctacg
     1381 gagagggagt aactgtcatc gaatgggcgg aaaggagcat gcccctgctt ccggccggga
     1441 cactgtttgt atatctcgct tatcggggag aaaatgatcg ggaattgatc ctgacgggga
     1501 ataatgaact gatcgggaaa ataaataact tgtgtaaaga gggaggttgc tgagaaatgg
     1561 cacttgtggt gcaaaaatac ggcggcacgt cggtggcgga tataaaacgc atcgaaaatg
     1621 ttgcccggag ggtgatcaag acaaaacaag cgggcaatga cgtgatagtc gttctgtccg
     1681 ccatggccgg ggaaacggac cggttgataa atctggctca tcaggcggcg gcggatcccg
     1741 attcccggga atacgacgcc ctgatctcca cgggggagca ggtaacggtg accctactgt
     1801 ccatggtact caacaggttg ggctataagg cccgttcgtt tctcagcttc cagataaaga
     1861 tcaataccga ccgggcgcat aaaaaggccc ggatcaccaa ggtggacacc cggaagatgc
     1921 gggaggagct gaagaaggga tcggtgctgg tcgtggccgg tttccagggg gtcgatgagg
     1981 aaaacgacat taccactctt ggccgcggcg gatcagatac gagcgccgtg gccctggcgg
     2041 cggccctgaa ggcggatctc tgtgaaatat acaccgatgt ggatggggta tatacgacgg
     2101 atccgaatat atacgacaag gcgcggcgtc tggaacggat ctcttacgat gaaatgctcg
     2161 aaatggcccg ggcgggagcc aaggttctac aaccccggtc ggtggaaatg gcgaaaaaat
     2221 atgaagttcc catttatgtc aaat
//
LOCUS       DAOSMF010000231         2175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_54584_length_2175_cov_16.935377, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000231 DAOSMF010000000
VERSION     DAOSMF010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54584_length_2175_cov_16.935377"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..1832)
                     /locus_tag="PLT63_11465"
     CDS             complement(42..1832)
                     /locus_tag="PLT63_11465"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOD99381.1"
                     /translation="MREKDFSIGEYIVGPASRRAVLPFAYPIIAADGRLQGIVAVSLD
                     LEKYGRSFLATTSFPKGSTINILDRNHIRLSRHSEQERHAGKVDLPDMVCHMSLGPPE
                     GVFIATGVDGVRRLFAYRRFSLQDGASPYLFMPVGVPEKLTLAASRENLVRNIALLTG
                     CVLAVMLAALLVANKLIVRRLNRLVERSRQLGRGDFTVCAGIDYREEELGKGDRIYAL
                     SFYGEKRPLLIDYIFDPQLSRQDNYNNFHRDGDVISAEGYVPFPGKEGRFLWAKAGPI
                     YDGVGKVIGAIEILRNITDERRLEEELRESEARFRMVIQHSGAVIAAVDLKGKFILVN
                     PAMVEMLGYSEEELLRMDFQQVSHPDDIAEEIEKAVRLLKGEIEGYRQEKRYIHKDGR
                     ILWAMFTATLVRDRQGKPHYFIGQAQDITDRIRAVETIRQMAYHDPLTGLPNRKLLAD
                     RMQMAQARAKRHGHLDAVAYLDLDGFKEVNDVHGHDIGDLLLREAAGRIPGVLRQGDT
                     MARVGGDEFVLVLPEIKDKSDVTAIAGKVMNCLRRPFLIGTHEIIITTSLGITFYPDD
                     GEEQEILLRNADDAMYQAKQAGKNTFRYYQ"
BASE COUNT          435 a          618 c          633 g          489 t
ORIGIN      
        1 ctggataacc taccggaatg gtggaattat ttcctctgga atcattggta gtaacgaaag
       61 gtgtttttcc ccgcctgctt ggcctggtac atggcatcgt cggcgtttct cagcaaaatt
      121 tcctgttctt cgccgtcatc gggataaaac gtgatcccga gactggtggt gatgatgatc
      181 tcgtgggtgc cgatgaggaa cggccgccgc aggcagttca tgaccttgcc ggcgattgcg
      241 gttacgtcac ttttgtcttt gatttccggt aggacaagaa cgaattcatc gccgccgacc
      301 cgcgccatgg tgtctccctg gcggagcacc ccggggatgc gcccggccgc ctcccgcagg
      361 aggagatccc cgatgtcgtg gccatggacg tcgttcactt ccttgaaccc gtccagatcc
      421 aggtaggcca ccgcatccag atgaccgtgg cgctttgccc gggcctgggc catctgcatg
      481 cggtccgcca ggagcttgcg gttcgggagc cccgtaaggg ggtcatgata ggccatctgg
      541 cggatcgtct ccaccgcccg gatccggtcc gtaatgtcct gggcctgacc gatgaagtag
      601 tgcggttttc cctgccggtc ccgcaccagg gtggcggtga acatggccca gaggatccgc
      661 ccgtccttgt ggatgtatct tttctcctgc cggtagcctt caatttcacc cttgaggagg
      721 cgcaccgcct tttcgatctc ctcggcgatg tcatcaggat gggagacctg ctgaaaatcc
      781 atccggagaa gttcctcttc cgaataaccg agcatctcga ccatggcggg attgacgaga
      841 ataaattttc ccttcaggtc cactgcggca atgacggcgc cggaatgctg aatcaccatc
      901 cggaagcgcg cttcgctttc tcgcagttcc tcttccagac gacgctcgtc ggtgatgttc
      961 cgcaagatct cgatggcgcc gatgaccttt cccaccccgt cgtaaatcgg tccggccttg
     1021 gcccagagaa atcgtccttc cttcccgggg aaaggcacat acccctcggc gctgattacg
     1081 tcgccgtccc ggtgaaaatt attataattg tcttgcctag acagttgggg atcgaagatg
     1141 taatcaatca gcagggggcg cttttcaccg taaaaggaca gggcgtagat ccggtcgccc
     1201 ttgcccagtt cctcttcccg gtagtcgatg ccggcgcaga cggtgaaatc gccgcgaccc
     1261 aattgccggg accgttccac gaggcggttg agtcttctta cgatcagctt gtttgccacg
     1321 agcagtgccg ccagcatgac ggcaagcacg caaccggtga gcagggcgat gttgcggaca
     1381 agattctccc tgctggcggc aagcgttagt ttttcgggaa cgccgaccgg catgaaaaga
     1441 tagggggacg caccgtcctg caatgaaaac cgtcgatagg caaaaagacg ccggaccccg
     1501 tcgacccccg tggcgatgaa cacaccctcc gggggtccta aggacatatg gcacaccata
     1561 tcgggcaaat cgacctttcc ggcgtgcctt tcctgttccg aatgccggga gaggcggata
     1621 tggttgcggt ccaggatgtt gatcgtggag cctttgggga aacttgtggt agctaaaaaa
     1681 ctccggccgt atttctccag gtcgaggctc acggccacga tgccctggag tcggccgtcg
     1741 gcggcgatga tcggataggc aaagggcagc accgcccgcc gggaagcagg cccgactatg
     1801 tattccccga tggaaaagtc tttttccctc attgtgtgct ggaaatatgc gcgttgcttt
     1861 atgatggagc cgcgagtgag cagggcgttg gtaaaaaacc attccctcta ggtccgccgc
     1921 atagatgttg gcatagtggt agttttcctt gatcagttcc cggaagaggg cgctgcaggc
     1981 cgacgcatac cgccgctgga ccatgggaag tttggagagg gtcatgacaa atcggcgcgt
     2041 gacttccacc acgctttcat gttcattcgc cagaccgcgg aggatcatca gggtgttggc
     2101 tttagccaga tccattttaa cagggggcat caattttaaa agtaaacctt gacagatgga
     2161 gaagcccctt tctat
//
LOCUS       DAOSMF010000232         2165 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_54915_length_2165_cov_24.325118, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000232 DAOSMF010000000
VERSION     DAOSMF010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54915_length_2165_cov_24.325118"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="PLT63_11470"
     CDS             <1..222
                     /locus_tag="PLT63_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99382.1"
                     /translation="LGHPGMQHPGPHRTAHQRPDQKLTAGWGPSSRPRDERIGKILIF
                     KIIQQKKPESLSYPQLPFFLTYFNRLKGM"
     gene            complement(208..639)
                     /locus_tag="PLT63_11475"
     CDS             complement(208..639)
                     /locus_tag="PLT63_11475"
                     /inference="COORDINATES: protein motif:HMM:NF024861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOD99383.1"
                     /translation="MKLFLDANIIFTAAYSAEGISRGLFRLATAGKCLLCTSAFAHEE
                     AARNIQKKAPDKLPELTRLMPQVAILPEPHPRRVAWAAGLPLARKDAPILAAAIQGKV
                     DILVTGDRRDFGCLFGQVLEGVTISTPAEAVEAIICKLHTF"
     gene            complement(636..881)
                     /locus_tag="PLT63_11480"
     CDS             complement(636..881)
                     /locus_tag="PLT63_11480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOD99384.1"
                     /translation="MQLVKLGKKGQLSLPKAILQQAGISGETPLLVDATDDGAIVLRP
                     AGVYPIEIYSDERIAAFLDEDRMTAEQMALLDSALKK"
     gene            complement(1001..1513)
                     /locus_tag="PLT63_11485"
     CDS             complement(1001..1513)
                     /locus_tag="PLT63_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99385.1"
                     /translation="MESSNHDREAFSLQARGLLDRGMYSVVRDLAETRLAEMPGDVEA
                     RTILCEALWGMGYDEEAQEIITEIEEIIRGYSEIYRLMGRICRDKGLAWEAIHYYERF
                     IDLNPHSPLRPEVEKDLDRLRTELGASGGEDHPAPIADREPPPAQMATLSKWLANLEN
                     KKKRKRPGKK"
     gene            complement(1665..1808)
                     /locus_tag="PLT63_11490"
     CDS             complement(1665..1808)
                     /locus_tag="PLT63_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99386.1"
                     /translation="MIEKSFWLQMIFFLCLFVGAPAGTAPAAAGERHPFTVTDLLAKG
                     TGY"
BASE COUNT          458 a          631 c          564 g          512 t
ORIGIN      
        1 ctcggtcatc cgggaatgca acaccctgga ccacatcgaa cggctcatca gcgccctgat
       61 cagaagctga cggcggggtg ggggccgtct tcccgccccc gggatgaacg catcggtaag
      121 atattaatat ttaagataat tcagcaaaaa aaaccggaaa gcctctcgta cccgcaactt
      181 ccgtttttcc tcacctattt caataggtta aaaggtatgt aatttacata ttatcgcttc
      241 cacagcttcc gccggcgtgg aaattgttac cccttccaag acctggccga acaggcagcc
      301 gaaatcgcgg cggtcgccgg tgacaaggat atcgaccttt ccctggatgg ctgcggcaag
      361 gatcggtgcg tccttccggg ccaagggcaa ccccgctgcc caagcgaccc gccttggatg
      421 cggttcaggc agaatagcaa cctgtggcat caatctggtc aactccggga gtttatccgg
      481 cgcctttttc tgaatatttc tggccgcctc ttcatgagca aaagcggatg tgcagaggag
      541 gcatttgcct gcggttgcta atcggaacag tccccgtgag atgccttcgg cgctgtaggc
      601 ggccgtaaaa atgatgttgg catcgagaaa caatttcatt ttttcagagc gctatcaagc
      661 aaagccatct gctccgccgt catccggtct tcatccagga aggcggcgat ccgttcatcg
      721 ctgtaaatct cgatgggata gacgcccgcc ggtcgcagaa caatggctcc gtcatccgtc
      781 gcatccacca gaagcggcgt ttcgccgctg atgcccgcct gctgcaatat tgctttgggc
      841 aacgatagct gtcccttctt gcctaatttg accagttgca tctcttctct cctggaaata
      901 agtattccga aaatccggat tccagataat gatacatatc aggatgtctc atggcaagaa
      961 aaatatggcg tccataagaa tgaagttaag ggaacgatga ttatttcttc ccggggcgtt
     1021 tccttttctt tttgttttcc aggttggcca gccatttact gagcgtcgcc atctgcgccg
     1081 gcggcggttc tcgatcggca atcggggccg gatggtcttc gcctcctgac gcccccagct
     1141 ccgtccggag ccggtccaaa tctttctcta cttccgggcg caggggggag tggggattca
     1201 ggtcgataaa ccgttcatag taatgaatcg cctcccaagc cagaccttta tcccggcaga
     1261 tccgtcccat gagccggtag atctccgaat atccccggat gatctcttcg atttccgtga
     1321 taatttcctg ggcctcctcg tcgtatccca tcccccagag ggcttcgcac aggatagtcc
     1381 ttgcctccac atcgccgggc atctcggcca gtctggtttc ggcgaggtcc cggaccacgg
     1441 agtacatgcc ccgatccagg aggccgcggg cctgcaacga aaaagcttcc ctgtcgtggt
     1501 tggaactctc cataatgtct tctccctttt gtcttgaccg ttgagaggcc gttatggcct
     1561 ccgcccgcat ccctcccggg cctcctggcg atccccgggt ccgtccagcc accggtggag
     1621 ccaggcgagc accgtttcat gccattggcg ggagtttggg ggggtcagta tccagtgccc
     1681 ttggccaaaa gatccgtcac ggtgaacgga tgcctttccc ccgctgccgc cggcgccgtc
     1741 cccgccggtg cgccgacaaa gagacagagg aagaagatca tctggagcca aaatgatttt
     1801 tcaatcataa taatcgacca ctccccccat cgtttttacc cgtagctata acatgcgggc
     1861 attgatttca caataatccg gaatgcggaa tgctttgact tttaaaggcg agttcaatat
     1921 atacgaatct cctgatttcg gtggtgcgcg gggagggaac cctttcacgg tctttgagga
     1981 cgaagctatg acggaagtga aaaaaaacac tcccttcgcc gagcagtgtt tggcggtaac
     2041 ggaatccctt ttccacagca tcgtggatca catgctggac gccctgctca ttctggactg
     2101 gaacggcgtc attctcttcg ccaatcaggc ggcggccgat ctggtccagt tggaaagccc
     2161 cgcgg
//
LOCUS       DAOSMF010000233         2160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_55081_length_2160_cov_16.589549, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000233 DAOSMF010000000
VERSION     DAOSMF010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55081_length_2160_cov_16.589549"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(297..1907)
                     /locus_tag="PLT63_11495"
     CDS             complement(297..1907)
                     /locus_tag="PLT63_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99387.1"
                     /translation="MKKGLFILLVLFLTALAAAPAVAVDVKFSGSYYTLGWYVDNPSH
                     LDKNEAPGAASWGTVDASQANYANRDARSRRGADAFYSQRFRLQTEFQVVEGLKLVTR
                     FDALEKRWGDQAWAGGTGETQSRTQTPQGSLDPNGVRRGAANPKAQENLEWERAYLDF
                     NVPFGKFQVGYMQFLTWGTTFLDTTLTSAGIRYLYSRGPWQAVAAIEKRGEWVGLNAN
                     NIGVANDADRDVYDFGAIYKFKPGEAGILYQYSRSGQNKWEQGNGYLTQLNGLYPYAK
                     ATFGKLFLEGEAMYAFGDLRSYESTSAAPTGGTAGTNRPGGVSINNPAQNVGVQAFGA
                     YVHGKYDLKPAYVGIKFAYLSGDDNSNSDKATGSIMQSQQMNRSFYPTLILWNDDYQN
                     NMGNIQGNVPAGATGANRRTGQNYTISQNMDNVWFYQIYAGVKPMPKMNLMMALSYAT
                     ADKKPKSDVGAVGSPGYYNPANRVTEFVSDKYGTELDFIGTYKIYDNLTYMIGIGYLW
                     TGDYFKGYDNDAKIRDNYLLSHKLTLNF"
BASE COUNT          408 a          641 c          597 g          514 t
ORIGIN      
        1 caggatgagg ttcttctgtt tcgccggcgc cttgcgggcg gcatcggcgc cgccgactcc
       61 ggccagaagg agtaaagtat agtatacagc gatcagaatg gcaatccttg catatctttt
      121 catttccatt tctccttaat tgaatctcga tgtcgatggc aggcgacctg ccctttgggt
      181 ttgtctaatc accgaacttg gggccggttt ccgatcgaaa acgaaccggg gcgccgcttg
      241 ccgacgcccc ggattttact tgtaattctc taccgttcca gacaaaggac ctgctcttag
      301 aagttcaggg tcaacttatg cgaaagaagg tagttgtccc tgatcttggc gtcgttgtcg
      361 taacctttga aatagtcccc cgtccacaga tagccgatgc cgatcatgta ggtgaggttg
      421 tcgtagatct tgtaggtgcc gatgaagtcg agctccgtcc cgtacttgtc tgaaacgaat
      481 tccgtcaccc ggttcgccgg gttgtagtaa cccggggaac ccacggcgcc cacgtcgctc
      541 ttcggtttct tgtccgccgt ggcgtaggac agggccatca tcagattcat cttgggcatc
      601 ggtttgacgc cggcatagat ctgatagaac cacacattgt ccatattctg gctgatcgtg
      661 tagttctggc cggtgcggcg attcgctccc gtcgcccctg cgggcacgtt gccctggatg
      721 tttcccatgt tgttctggta gtcatcgttc cagaggatca gggtgggata gaagctccgg
      781 ttcatctgct gggactgcat gatagatccc gtcgctttgt cgctgttgga gttgtcgtca
      841 ccgctcaaat aggcgaactt tatccccaca taggccggct tcagatcgta cttaccatgg
      901 acataggccc cgaaagcctg gacgcccacg ttctgagcag gattgttgat gctcacccca
      961 cccggacggt tggtgcccgc ggtaccgccc gtgggggccg cactggtgct ctcgtaagat
     1021 cgcagatcac cgaaggcgta cattgcctct ccttcgagga acaacttccc gaaggtggcc
     1081 ttcgcatagg gatacaggcc gttgagctgc gtcagataac cgttgccctg ctcccatttg
     1141 ttctggccgc tccggctgta ttgatagaga atgcccgcct cgccgggttt gaatttatag
     1201 atggccccaa aatcgtacac atcccggtcg gcgtcattgg ccacgccgat gttgttcgcg
     1261 ttgaggccta cccactcccc tcgcttctcg atggccgcca ccgcctgcca gggcccgcgg
     1321 ctgtaaaggt agcggatgcc ggcggaagtc agggtcgtgt ccaggaaggt cgttccccag
     1381 gtaagaaact gcatgtaacc cacctgaaac ttgccgaagg ggacgttgaa atccagatag
     1441 gcccgttccc attccaggtt ttcctgagcc ttgggattgg cggcgccgcg tctcacccca
     1501 ttgggatcga gagatccctg gggcgtctgg gtccgggact gggtctcacc cgtgcccccc
     1561 gcccaggcct gatcacccca gcgcttctcc agggcgtcga agcgggtaac gagcttcaaa
     1621 ccttccacta cctgaaattc cgtctgcaaa cgaaaccgct ggctgtaaaa ggcatcggcg
     1681 ccccgccgac tccgggcgtc ccggttggcg taattggcct gtgaggcgtc cacggtgccc
     1741 cacgaggcag cccccggcgc ctcgttcttg tccagatgcg acgggttgtc cacataccag
     1801 cctagcgtgt agtagctgcc gctgaacttg acgtccaccg ccaccgcggg agcggcggcc
     1861 aaggccgtca ggaacaggac aagtaaaata aatagacctt ttttcacttt taacctccta
     1921 atcttattac ccattattac ctacagcggg ttgttgtgcc aagtatctgt cctggcgacc
     1981 gctatttgcc gccgccacgc tgccgccagg gaatcgaatc ggggaagaac atgggtttcc
     2041 cgtatttttc caccccgaag gtctcaatga accgctgtcc cttgttcgga tccgtgagcc
     2101 aatcgacgaa gatcatcgtt tctttgaggt tggcctgggg acatttcccg ggattcaccg
//
LOCUS       DAOSMF010000234         2143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_55708_length_2143_cov_20.843391, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000234 DAOSMF010000000
VERSION     DAOSMF010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55708_length_2143_cov_20.843391"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            543..1343
                     /locus_tag="PLT63_11500"
     CDS             543..1343
                     /locus_tag="PLT63_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99388.1"
                     /translation="MLIQIYEIQTPREAEQMIALGVDHIGSVVLDRDNWRDPTLREAV
                     RLTAGTDARSSLIPLFSDVDAVSRLLDYYRPDIVHFCENLIPEPGLGPEAPGGWGAAT
                     GKIVDLQRFVRDKFPDVKITRSLPIPAAAMPRAGLAALVEMFAPVSDFFMTDTLLTGP
                     GGAGGDAAQPVTGFVGITGMTCDWEAAAWLVRTSPLPVILAGGLGPENAAAAIARVQP
                     AGVDSCTRTNAVDAQGRPIRFRKDISKVRDFVATARRAAATIGEPLKR"
     gene            complement(1403..>2143)
                     /locus_tag="PLT63_11505"
     CDS             complement(1403..>2143)
                     /locus_tag="PLT63_11505"
                     /inference="COORDINATES: protein motif:HMM:NF014635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HOD99389.1"
                     /translation="KLKTSPVAVKFLKSKEDFPDKTRRPSVVLGKKIAVCQAVTMARL
                     YGWTVGLTKEDIVCIPAAFAFGYSDAEDSAAALGKLFGIGSYTQTEEIGRAEAQTIHR
                     LARGEYEAILLAPLARATFAPDTIVFYGNPAQIMKLVQGWTYDKGKRVEGHFGGKVEC
                     TEYLLAPFRTQTPRIAIPGTGDRIFSMTQDDEIVFAIPGGRLAELVQALPEAGKKVGA
                     VYPVPVFLNFQPEFPKQFRELGKELGVS"
BASE COUNT          429 a          630 c          645 g          439 t
ORIGIN      
        1 tatatcattc cttaaattgc cttcgtcggt aacacatggc gccgggcttg tcaagcaggc
       61 tctgtaccgg cgttcccggc ccgcggcggg cgaaagaata ccagcatgtt ttcgccttga
      121 tgtagacaac ttccgtggtt gttgccgcat tcggcatagg ccattctcgg atgccccctt
      181 tcgatatgtc ttagaaaaaa atcatatgga tactgattgt ttcgctgccg ccgatggttt
      241 taaatatcca ctccgcaagg atatgttcca tcttcccgag cggaagtgga gaattgagaa
      301 aggagagcgc aaaaatgacg acccagaaaa aaaaggtacc ccaaaatgca cccgaatcca
      361 cagaggataa taagaccttt aaaccctata aatgtcagat gtttctgaga tattacgggc
      421 cttgagggtt agttcgggca tcgtgatggt attggcggcg gtaaaccttt accgtgtcgc
      481 ggacatgccg gcttgccttt ccgggaagaa tgtattatct gcccaccgga tagggggaag
      541 cgatgctcat tcagatctac gaaattcaga cgccccggga ggcggaacaa atgatcgccc
      601 tcggggtgga ccatatcggg agcgtcgttc tggaccggga taactggcgc gatccgaccc
      661 ttcgggaggc ggtccgtctg accgcgggga cggacgcccg gagcagcctg atccccctgt
      721 tcagcgacgt cgatgcggtg tcacggcttc tggattacta ccgaccggat atcgtccact
      781 tttgcgaaaa cctcattccc gagcccgggc ttggtccgga ggcgccgggg ggatggggcg
      841 ccgcgacggg aaaaatcgtc gatctgcaaa gatttgtaag agataaattc ccggacgtga
      901 agatcacccg ctccctgccc atcccggcgg cggcgatgcc ccgggccggg cttgccgccc
      961 tggtggagat gtttgccccg gtgagcgatt tcttcatgac cgatacgctc ttgactgggc
     1021 cgggcggcgc cgggggcgat gctgcgcagc cggtgaccgg ttttgtgggt atcaccggga
     1081 tgacatgcga ctgggaggcg gcggcctggc tggtccggac cagtcccctg ccggtgatcc
     1141 tggcgggagg cctggggccg gaaaacgccg ccgccgccat cgcccgggtg cagccggccg
     1201 gggtggacag ttgcacccgg accaacgccg tggacgctca gggccgcccc atccgtttcc
     1261 ggaaggatat cagtaaggta cgggactttg tcgccaccgc ccggcgggcg gcggcaacca
     1321 ttggcgaacc cctaaaaaga tgactccggc agacgcttac gcggctgaca gcatgaattg
     1381 ttatacacgg cgaacgacct cgctacgaaa cccccaactc tttacctaac tccctgaatt
     1441 gctttgggaa ttccggctgg aagttgagga aaaccgggac cggatacacg gcgcccacct
     1501 ttttccccgc ttccgggagg gcctggacca actccgcgag ccggccgccg gggatggcga
     1561 agacgatctc gtcatcctgg gtcatagaga agatccgatc tccggtgccg ggaatggcga
     1621 tccggggcgt ctgggtccgg aaaggcgcca ggaggtactc cgtgcattcc accttgccgc
     1681 cgaaatggcc ctccacccgc ttacccttgt cgtaggtcca tccctggacc agtttcatga
     1741 tctgggccgg attgccgtaa aatacgatcg tatcgggtgc gaaggtcgcc cgcgccagcg
     1801 gggcgaggag gatcgcttcg tattctcctc gggcaagtct atgaattgtt tgtgcttccg
     1861 cccggccgat ctcttcggtt tgcgtgtagc tgccgatgcc aaacaatttc cccagggccg
     1921 ccgctgaatc ctccgcatcg ctgtacccga aggcgaaagc cgccgggatg caaacgatgt
     1981 cctctttggt aaggccgacg gtccagccat aaaggcgcgc catggtcacc gcctggcaga
     2041 cggcgatctt tttacccagc accaccgacg gccgccgcgt cttatcggga aaatcctcct
     2101 tgcttttcaa gaacttgacc gccacggggg acgtcttgag ttt
//
LOCUS       DAOSMF010000235         2132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_56096_length_2132_cov_20.580164, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000235 DAOSMF010000000
VERSION     DAOSMF010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56096_length_2132_cov_20.580164"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            387..1202
                     /locus_tag="PLT63_11510"
     CDS             387..1202
                     /locus_tag="PLT63_11510"
                     /inference="COORDINATES: protein motif:HMM:NF022586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2889 domain-containing protein"
                     /protein_id="HOD99390.1"
                     /translation="MNLFGKPRGKKYHTRVAEVTTYEYDVRRLAIEGRLTDRRFQDSH
                     LATGEKRAPGILHQMIIRLLVDRETLAIEDLQVEMPGVPRDECREALESLEPVKGRRI
                     ARGFTSQAKALSGGGRGCNHLTALLAVMGSAAIQGYAAAREDGEPVPIADLIAILVNG
                     CWTWREEGPLISQLRELSPPHEKRELTAPHGKREFTLPRGENIHQRERKMGMDEKERT
                     VMETMKKEGKPMRPGDIAKKAALDKTEVTKIIDKLKKEGKVIVPKRCFYAPAE"
     gene            1504..1905
                     /locus_tag="PLT63_11515"
     CDS             1504..1905
                     /locus_tag="PLT63_11515"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOD99391.1"
                     /translation="MIAFANESMAVNRYSYFSKAAKKEGLEMIADIFSKISREEKSHA
                     SVFYRFFEGGAVEVTATFPAVRVADTKSNLEAAIKGENATWTLRYPDFERTAREEGFF
                     KLADIFQRFANAEKKHADIFQQVLAELGSQT"
BASE COUNT          574 a          521 c          612 g          425 t
ORIGIN      
        1 tccacccgca agccggcgga gattttgccg ccgcgccgct cccgcacggg gcgcggccag
       61 gggaacataa tacaaggaac cggcgggggc aagccaaatc tcccgcccag tcgcccccgc
      121 ccgggacgga gattggattt ctcccaggat gatgatatga ggaactcgta aaaaatcatt
      181 attcacacgg cgaagtaaaa gatccagagg caaggcgcgc gaatcctgag gaatgagaag
      241 tacttgggcg tacgtcgcag taactaaaga tgaagcgctt tcgccgcata tggacttttt
      301 acgatgctgt catgatatga tcggagcccg tcgccggcga aatcgccgcc ccgtcaccgg
      361 gggcgcggga aaagaaagga tacgtcatga atttgttcgg aaaaccccgg gggaaaaaat
      421 atcatacccg tgtcgcggag gtgaccactt acgaatacga cgtccggagg ctggccatag
      481 agggacggct gacggatcgc cgctttcagg actcccacct ggccaccggc gagaaaaggg
      541 cgcccggcat attgcatcag atgatcatcc gcctgctcgt ggacagagaa accctggcga
      601 tagaagacct gcaagtggag atgcccggcg tgccccggga cgaatgccgg gaggctctcg
      661 aaagcctcga acccgtcaag ggacggagaa tcgcccgggg cttcacgtcc caggcgaagg
      721 ccctgagcgg cggcggccgg ggatgcaatc atctgacggc gctccttgcc gtgatgggtt
      781 ccgcggccat acagggatac gccgcggcga gagaagacgg ggagccggtg ccgatagcgg
      841 atctaatcgc gatcctggtc aacggctgct ggacatggcg ggaggaaggc cccttgatca
      901 gccaattgcg ggagctttca ccgcctcatg aaaaacggga gcttacagca ccccacggga
      961 aaagggagtt tacgctgccc cggggggaaa acatacatca aagagaaagg aaaatgggta
     1021 tggacgaaaa ggaaagaacg gtcatggaga cgatgaagaa agagggaaag cccatgcgac
     1081 ccggagacat cgccaagaaa gcggcgctgg acaagaccga ggtaaccaag atcatcgaca
     1141 agctgaagaa agaggggaag gtgatcgtgc cgaaacggtg cttctacgcc ccggcggaat
     1201 gaagcctcgg aaccgtttct gaccgccggt cattttttcg ggggcaaaat tttttcaaat
     1261 tctcagattc cttttattta tcgtagggtt gctgagcgtc gattctcgtc acgccccggc
     1321 ggaagcaccg aaatgctgtc tatttctcat aatgacgcta ataccatgct catatatttc
     1381 gtggacggaa acaactttct aacatgttta tttgcttacg gtttttgctt ttgactttca
     1441 cctataagga ggagttgagc aatgataaag acaataaaag gatcaacaac agagattaat
     1501 ctgatgatcg cctttgccaa tgaatcgatg gccgtaaatc gctattcata tttttccaaa
     1561 gccgcgaaaa aggaagggct ggagatgata gccgatattt tctcgaaaat atcgcgggaa
     1621 gagaaaagtc acgcttccgt tttctacaga tttttcgaag gcggcgccgt ggaggtgacc
     1681 gcaacatttc ccgccgttag agtggcggac acaaaaagca atctggaagc cgctatcaag
     1741 ggtgaaaacg caacgtggac gcttcgttat cccgactttg aacgaaccgc ccgcgaggaa
     1801 ggttttttca aactggcgga tatttttcaa cgcttcgcaa atgccgagaa aaagcatgcg
     1861 gacatattcc agcaggttct tgcggagctg ggaagtcaaa cttgagatat cagccaaggt
     1921 tctgaacaag cccgccgcgg cggtgaatgc gggtcgaaac attccgcaga gggcagatga
     1981 ttgttgagga caattcctat ctgttagaat aaaggggaat agctctggat tgatatttcg
     2041 cgtaatattc gtctgtcatc cagagggaaa taccatggca atgtgcatct taattgctga
     2101 cgataatcac aataatttgc acctgctgga aa
//
LOCUS       DAOSMF010000236         2111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_56861_length_2111_cov_13.063230, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000236 DAOSMF010000000
VERSION     DAOSMF010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56861_length_2111_cov_13.063230"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..917
                     /locus_tag="PLT63_11520"
     CDS             105..917
                     /locus_tag="PLT63_11520"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HOD99392.1"
                     /translation="MKEAGFPLRELKKGTHALNKDILKNLEVFDDTELSLVVALKNMV
                     GQLGSPFQQAADELFSRLCDASANMPVFVKIDDAGTMDARLINRVIKAIRERKQVAFR
                     YAAHAPHDVSLEPYRIAYYQGFWYLVGLDCDAKLIKRYAVDKIEDLKTTKICCKGAPP
                     TLDDAMRNSANIWFADERNLEVTVQVDGSCSDYFKRRRIYPTQEIREEKPDGSLIVSF
                     QVGHFDAVRHLLKAWLPHVIILEPVSFRNEMLADMRKWVRRQGAEAPCRKSV"
     gene            complement(998..1417)
                     /locus_tag="PLT63_11525"
     CDS             complement(998..1417)
                     /locus_tag="PLT63_11525"
                     /inference="COORDINATES: protein motif:HMM:NF027249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HOD99393.1"
                     /translation="MSVKEVKAITPPYPFEAYAHVVSPLSPAEGGGFLISFPDLPGCM
                     SDGETIEEAIQNGRDAFLSWISAQADMGREIPKPVYRAPETEPPMSGKFVQRVPKTLH
                     AKLAALARQEGVSLNTLVLTFIAEGVGMREDKRHAQT"
     gene            complement(1414..1659)
                     /locus_tag="PLT63_11530"
     CDS             complement(1414..1659)
                     /locus_tag="PLT63_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99394.1"
                     /translation="MSGADKLIARMRNNPRDWQIDTLVSIADKHGIEVRSHGGSHFIF
                     SHPQVSFHPSVPAHRPIKPVYVRQFIDLIDAVKELQK"
     gene            complement(1753..>2111)
                     /locus_tag="PLT63_11535"
     CDS             complement(1753..>2111)
                     /locus_tag="PLT63_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99395.1"
                     /translation="ILARLTALNRERVQEEKEGVIRWLRPEFQNPYGTATAVQTEMTL
                     VREDKPVPLKVPLPKDHTGQLQAVRTALAAFKGPVAPDELAQCFLKARTARVTTLLDD
                     LVKLGLGRKEGEKYVA"
BASE COUNT          471 a          557 c          587 g          496 t
ORIGIN      
        1 ttgtccagaa tcaagaccat tatacgtaac cgtaattata aattgaggta ctaagcaatc
       61 atttccatac gacgccaaga acaatccaga gggatttgtt gctgttgaag gaagcgggat
      121 tcccgctccg ggagctgaaa aagggaaccc acgccctgaa taaggatatt ctcaagaacc
      181 tggaagtctt cgacgatacg gaactttccc tggttgtggc gctgaaaaac atggtgggcc
      241 agttgggatc gcccttccag caggcggcgg atgagctgtt cagcaggttg tgcgatgctt
      301 ccgccaacat gcctgtattt gtcaagatcg atgacgccgg gacaatggat gcccggctca
      361 tcaaccgcgt aatcaaagcc atccgggaaa gaaagcaggt cgctttccgc tatgctgccc
      421 atgcgcccca cgacgtaagc ctggagccct atcggatcgc ttattatcag ggtttctggt
      481 atctcgtcgg tctggattgc gacgcaaagc tcattaaacg ctatgccgtc gataaaattg
      541 aggatttgaa gacaacgaag atatgctgca agggggcgcc gccaaccctg gacgacgcca
      601 tgcgcaacag tgccaacatc tggttcgccg atgagcgtaa cctggaagtg accgtgcagg
      661 tggatgggtc atgcagcgac tatttcaaac gccgccggat ctaccccacc caggagatca
      721 gagaggaaaa gccggacggg tcgctgatcg tttctttcca ggtcggacat ttcgacgccg
      781 tccgccatct cctcaaggct tggctgcccc atgtgatcat tctggaaccg gtatcattca
      841 gaaatgagat gttggcggat atgcggaagt gggtccggcg tcagggagcg gaggcgccgt
      901 gccgcaaatc ggtttaaacc tttttcattg tcatgacaat ataaacaggg aggttttgtt
      961 cccggctttt ttattctcga cgcctgtttg tctgtgatta tgtttgcgcg tggcgtttat
     1021 cttcccgcat ccccactccc tcggcgataa aggtaaggac cagggtattg aggctgacgc
     1081 cctcctgtct ggcgagggcg gcaagtttgg catgaagcgt tttcggcacc ctctggacaa
     1141 acttgccgga catgggcggt tccgtctccg gggcgcgata gacaggcttg ggaatttccc
     1201 tgcccatatc cgcttgcgcg gaaatccagg acagaaaggc atctctgccg ttctgaatcg
     1261 cctcttcgat cgtttccccg tcggacatgc acccgggaag atcgggaaag gaaataagaa
     1321 aaccgccgcc ttccgcaggg gaaaggggac tgacgacatg ggcataggcc tcgaaaggat
     1381 agggaggcgt gatggccttg acttctttga cactcatttt tgcaactcct tcacggcgtc
     1441 aataaggtcg ataaactgtc tgacatacac tggtttaatc ggtctgtgcg ccggaaccga
     1501 cgggtgaaaa gatacctggg gatgtgaaaa gatgaaatga ctgccgccgt gacttctcac
     1561 ttcgatgccg tgcttatcgg caatactgac cagcgtatca atctgccagt cgcgggggtt
     1621 gttcctcatt ctggcaatca gtttatcggc gccgctcatg gtttgtatga tactaaatgt
     1681 aatatcattt gtcaatgggc aattccatga tcttaatgca gaaaaatcgg aagaagtgat
     1741 gtctgccgat attcacgcta catatttctc cccttctttt ctccccaggc ccaacttcac
     1801 cagatcatcg agcagcgtcg tcaccctggc ggtgcgcgcc ttgaggaagc attgggccaa
     1861 ctcgtcgggt gcgacggggc ctttgaaagc agcaagggcc gtccggacgg cctggagttg
     1921 ccccgtatgg tctttgggca aggggacctt cagaggtaca ggcttgtcct cccggaccag
     1981 cgtcatctcc gtctgcaccg ccgtcgccgt cccatagggg ttctggaact caggccgcag
     2041 ccagcggatg acgccttcct tttcctcttg gacccgttcc cggttcaggg cggtgagacg
     2101 tgccaggatt t
//
LOCUS       DAOSMF010000237         2110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_56895_length_2110_cov_28.131873, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000237 DAOSMF010000000
VERSION     DAOSMF010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56895_length_2110_cov_28.131873"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            205..495
                     /locus_tag="PLT63_11540"
     CDS             205..495
                     /locus_tag="PLT63_11540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99396.1"
                     /translation="MKMLCVIYADYFDQTITEIFKQAGCKRYIKLHDETGEDESYDAR
                     LGSHTAAGKLKTIFMDAPDEEITRLLDIVRRLKADYPSVGMRAFTFPIEEFI"
     gene            complement(686..1627)
                     /gene="miaA"
                     /locus_tag="PLT63_11545"
     CDS             complement(686..1627)
                     /gene="miaA"
                     /locus_tag="PLT63_11545"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOD99397.1"
                     /translation="MSEKPNLVVVLGPTAAGKTRLAAHLARERGGEIISADSRQVYRG
                     MDIGTGKDRRDYVAEGAAVPVHLLDVADPREEFHLFAYQGLFYRAFSEISARGALPIL
                     VGGSGMYLEAAIRGYEMAAASENLELRRELAALDMGELAKRLLSMQPELHNRTDLVDR
                     HRLVRAIEIATAARGGEGRPGVERPVLRPLVIGVRWERDVLRRRITARLHERLAAGMV
                     EEVTTLHQSGVGWERLDRFGLEYRYVGLYLQGRLDYDEMVRTLNTRIHQFAKRQDTWF
                     RRMERRGVTIHWIPGGDYAGLRSLVNREMGPGKQEGR"
BASE COUNT          505 a          589 c          549 g          467 t
ORIGIN      
        1 tgtatcatgg cttgttgcat ataggaccag ggtgagggcc agatcgcgac tcaaatcatc
       61 ggcaatgatg gcattggaaa cacgccagaa tccattgaca cacagagttg aatatcatat
      121 actccctata aaaaagggga gtgtttcctc agatgcactg aaatgttgtt tcttgagaat
      181 attttttaaa atgaggtcga acttatgaaa atgttatgtg ttatctatgc tgattacttc
      241 gatcaaacca tcaccgagat atttaagcaa gcgggttgta agagatatat taaattgcat
      301 gacgagacgg gtgaagacga atcatatgat gcaaggttgg gtagccacac ggcagcaggc
      361 aagctcaaaa ccattttcat ggatgcgccc gatgaggaga ttactcgcct tcttgatatt
      421 gtacggagac tgaaagcaga ctatcctagt gtcggtatgc gggcgtttac ttttccgata
      481 gaagaattta tttagtagat aaccgtctga cagaaaattg acatactatc tttcgggaaa
      541 ttgcggaggc gcaatttgaa tcaaaatatg gcatttattt ctgtgaatgt ttcgacccgc
      601 attaattgac ccaaccccat tgccaaaacg cttgctttgt atttgaagtg caactttgat
      661 gaaggcggta aacgttgcct gagcgctatc ttccctcttg cttgcccgga cccatttccc
      721 tattcaccag ggagcgcagg ccggcgtaat cgccgccggg aatccagtgg atagtcaccc
      781 cccggcgctc catccggcga aaccaggtat cctggcgctt ggcgaactgg tggatccggg
      841 tattgagggt tcgcaccatc tcgtcgtagt ccaggcggcc ctggagatac aggccgacat
      901 aacggtactc caagccgaag cggtccaggc gctcccaacc gacgccgctt tggtgcagtg
      961 tcgtcacctc ctccaccatc cccgcggcaa gtcgttcatg cagccgcgcg gtgatccggc
     1021 gccgcaggac atcccgctcc cagcggacac cgatgacgag agggcggagc actggacgct
     1081 ccactcccgg ccttccctcc cctccccgcg ccgccgtggc gatctcgatc gcccggacaa
     1141 gccggtggcg gtccacaaga tcggtccggt tgtgcaactc cggctgcatg cttagaagcc
     1201 gcttcgccaa ttcccccata tcgagggcgg ccaactcacg gcgcagttcc aggttctccg
     1261 acgccgccgc catctcgtaa ccgcggatcg ccgcctccag gtacatgccg ctgcctccca
     1321 cgagaatggg cagcgccccc cgggcggata tctcggaaaa ggcccggtaa aagagcccct
     1381 gataggcaaa aaggtgaaac tcctcccggg gatccgccac gtcaaggaga tgcacgggaa
     1441 cggcggcacc ctccgcgacg taatcccgcc ggtccttccc cgtccctatg tccatccccc
     1501 gatagacctg gcgggaatcc gcggagatga tctcaccgcc ccgttcccgg gccagatgcg
     1561 ccgcaagccg ggttttcccc gccgctgtag gtcccagaac gacgaccaga ttcggttttt
     1621 cactcatatt cccatctcca gcgaccgttg aaaaatactt cctatttacc gccttttcgg
     1681 ctaaagcaca ccgcgggttt gaagacacgg acatccggcc cgtccgggcg tgtcaagtgc
     1741 cacgatcgag ggcggctaca cggacgattc ctatacaggc gggatacttc gtgtcaagcg
     1801 aagagggcga taagctgaaa acatatcgac atcattgatg aattcgaaaa aagtcatgat
     1861 ggagacgacg aagtaaaaag ctccagaggc gaggcgcgcg aatcctgagg aatgaggcgt
     1921 acttgtgcgt acgtcgcagt gacgaaggat gaagcgcaac gccgcatatg gactttttac
     1981 gatgtcgtca tcattgatca ttcatcggaa tcatccgtgg ttgccatggc gtgaaagaaa
     2041 cgtcatctct ttaaccttta tatgaaaatg tccacaagta gccggatatt ttcatctttc
     2101 gttgtgaccg
//
LOCUS       DAOSMF010000238         2095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_57473_length_2095_cov_21.364706, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000238 DAOSMF010000000
VERSION     DAOSMF010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57473_length_2095_cov_21.364706"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            123..2018
                     /locus_tag="PLT63_11550"
     CDS             123..2018
                     /locus_tag="PLT63_11550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOD99398.1"
                     /translation="MKARVLIVDDNSAGRYLLESILKGFGLEVVAAENGREALEKARS
                     HPPDLIVADILMPVMDGYTLCREWKADAALKHIPFVFYTATYTEARDEAFALDLGADS
                     FIVKPQEPEALMDILRQVLAKSQETAPAAPAKPCGEEMEFFRQYNTILFHKLEKKMED
                     LEIANENLNALNERYRLFFDHATDVIYTIGADLKITSVSPSMERILGYKPREFIGRSV
                     ADLGRVVLREDMEKAIADIKAILAGNTIPTAVYRFIASDGKIKYGEVSGSPIMHDGRV
                     KGIISVARDITARKLAEEQLRKSERMYRDLYDFLPIPVYEMDFEGNITAANRAIYDAF
                     GGTEDDLRKGFNAWQLLPPEDVARAVENIQRLLKGEPLPSTEYNIKRLDGKAFPAIII
                     SSIIFRDGKPAGLRGAVIDITERRRYEKQLQSMLDNLQKAVATTIQVMASAVEARDPY
                     TAGHQLRVAELAQAIAREMGLPQEKIDGIRMAGSIHDIGKLSVPAEILSKPTKLNEIE
                     FSLVREHSRKGYEMLRTVDSTWPLAEVVFQHHERMDGSGYPRHLRGEEIIIEARIIAV
                     ADVVESMSSHRPYRPSLGIEAALEEIEAHRGILYDAPAVAACLKLFREKKFQFKGIEL
                     YGASLQK"
BASE COUNT          526 a          521 c          623 g          425 t
ORIGIN      
        1 gtgaaacgat cgtcaaaatt gtggaaagca cccgccccct ggcggacaaa aaagggctgg
       61 aaatgactgg tgccattttt ctctgcccaa agcggggaaa ggataaacgg gggtaagaga
      121 tcatgaaggc aagggtcttg atcgtggacg ataacagcgc cgggaggtat ttgctggaaa
      181 gcatcctgaa aggattcgga ctggaggtgg ttgcggcgga aaacggtcgg gaggcgttgg
      241 aaaaggcccg cagccaccca ccggacctga ttgtcgccga catcctcatg ccggtcatgg
      301 acggctatac cctatgccgg gaatggaagg cggatgccgc cctgaaacac atccccttcg
      361 tgttttacac cgccacatat acggaggccc gagatgaagc atttgccctt gatctcggag
      421 cggattcatt catcgtcaaa cctcaggaac cggaagcgct gatggatatt ttgcggcagg
      481 tgctggcgaa gagtcaagag acggcaccgg cggcgccggc gaaaccctgc ggggaggaga
      541 tggaattttt ccgccagtat aacaccatcc tcttccataa gctggaaaag aagatggagg
      601 atctggagat cgccaatgag aacctcaacg ccttgaatga gcgataccgg ttgttttttg
      661 accatgccac ggatgtcatt tatacaatcg gggcggacct aaaaattacc agcgtctctc
      721 cgagcatgga gaggatattg ggttacaagc cccgggaatt catcggtcgt tcggtagccg
      781 atttaggaag agtggttctg cgggaagata tggagaaggc cattgcagat atcaaagcga
      841 tcctggcagg caacaccatc ccaacggcgg tttatcggtt catcgccagc gacggcaaga
      901 taaaatatgg cgaagtgagc ggctccccca ttatgcatga cggcagggtg aaggggatca
      961 tctccgtggc ccgggacatc acggcccgca agctcgcgga ggagcaactt cggaaaagcg
     1021 aaagaatgta tcgagatcta tatgattttt tgccgattcc cgtttatgag atggattttg
     1081 aaggcaacat caccgcggcg aaccgggcga tctatgatgc tttcggcggc acggaagatg
     1141 atttacgaaa gggatttaac gcctggcaac ttctgccccc ggaagatgtc gcgagggccg
     1201 tggagaacat ccagcggttg ttgaagggag aaccgttgcc gagcacggag tacaatatca
     1261 agaggttgga cgggaaggct ttcccggcca tcatcatttc gagcatcatt ttccgtgatg
     1321 gcaaaccagc gggactaaga ggcgcggtca tcgatatcac cgaacgccgt cgttatgaaa
     1381 aacaactcca aagcatgctc gataatctcc agaaggcggt cgccaccacc attcaggtca
     1441 tggcctccgc cgtcgaggcc cgagatccat atacggccgg acatcagctc cgcgttgcgg
     1501 aacttgccca agccattgcc agggaaatgg gcctgcccca ggaaaagatc gacggcatcc
     1561 ggatggcggg ttccatccac gacatcggaa aattgtccgt ccccgcggag atattgtcga
     1621 aacctaccaa gcttaacgaa attgaattct ccttggtgcg ggagcattct cggaaaggat
     1681 acgagatgtt gcggacggtg gattccacct ggccactggc ggaggtcgtt ttccagcacc
     1741 atgagcggat ggatggttcc ggatatcccc gtcatttgcg gggggaggag atcattatcg
     1801 aagcgcgcat catcgccgtt gccgatgtag tggaatccat gtcgtcccat cgtccttatc
     1861 ggccgtcgct gggcatagag gcggctctgg aggagatcga agcccaccgg gggatccttt
     1921 atgacgctcc ggcggtggcg gcctgtctta aattgttcag ggaaaaaaag ttccaattca
     1981 aaggaataga attatacggc gcgtcgcttc aaaagtaagg gcggccgggc gtttattgag
     2041 gaattcgtaa aaagcgccct agcccgccga ctgccgggcc tgtataaaag aagta
//
LOCUS       DAOSMF010000239         2060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_58793_length_2060_cov_23.752618, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000239 DAOSMF010000000
VERSION     DAOSMF010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58793_length_2060_cov_23.752618"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /locus_tag="PLT63_11555"
     CDS             complement(<1..739)
                     /locus_tag="PLT63_11555"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HOD99399.1"
                     /translation="MNDRTVVKFTDVNFSYNGAPVLAGVQFAIPARTFISIVGPNAGG
                     KTTLLKLMLGLLKPDRGRIEIFGLPPVKARPRIGYMPQHVQFDSRFPVSVLDVALMGR
                     LGAGAGIGPYRKADREAALEALRRLEMEAFRRRPFTDLSGGQRQRVLIARALAAEPEL
                     LLLDEPTANVDMAVETELFELLHVMSADITIVVVSHDLGFVSQYVESVICVNRRVMVH
                     PTAAVTGEVISALYGADVRMVNHRHGLQ"
     gene            complement(770..1804)
                     /locus_tag="PLT63_11560"
     CDS             complement(770..1804)
                     /locus_tag="PLT63_11560"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HOD99400.1"
                     /translation="MFGRLLCCCCLAGVLLAVPAPLLRGQEGGGSSPGPTPRLPIFVT
                     ILPQAYFVERIGGRHVDVSVLVGEGQSPHSYEPTPRQMARLSGARAYFRIGVPVERAV
                     LGKIGASHRKIEIVATQQGVPFRYPDEEGHDNGGNDGKGKPRKVKEKTDASTHEQGRT
                     SARGGKTAGKPGAPPHPAGGRRAPDPHIWLDPRLVKIQARNIHDALCRLDPAHRQEYG
                     ANLQAFQADLDRVDARIARALAPLKGKKLYVYHPSFGYFAAAYGLIQTPVERGGREPS
                     ARQLASLIAAARRDGVKVIFVQSQFPAKSAAAVARAIGGVVVPIDPLARDYLDNLNRI
                     AAAVEQGLGL"
     gene            complement(1821..>2060)
                     /locus_tag="PLT63_11565"
     CDS             complement(1821..>2060)
                     /locus_tag="PLT63_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99401.1"
                     /translation="KVNEDLREVISNIPNRSEFIRAAIMTALENVCPLCNGTGILSPK
                     QKEHWEGFAQNHAIRRCEDCHELFIECANVVAKPE"
BASE COUNT          345 a          660 c          677 g          378 t
ORIGIN      
        1 tttgcagccc gtggcggtgg tttaccatgc ggacgtcggc gccgtagagg gcgctgatca
       61 cctccccggt caccgcagcg gtgggatgga ccatgacccg gcggttgacg cagatgacac
      121 tctccacgta ctgggaaacg aagccgagat cgtgactgac caccacgatg gtgatgtcgg
      181 cgctcatgac gtggaggagt tcgaacaatt ccgtttccac cgccatatcc acgttggccg
      241 tcggttcgtc cagaagcagg agttccggct ccgccgccag ggcccgggcg atgaggaccc
      301 gctggcgctg tcctccggag aggtcggtga aggggcgcct ccgaaaggct tccatctcca
      361 ggcgtcgcag ggcctccagc gccgcttcac ggtcggcctt ccggtagggc ccgatacccg
      421 cgccggcgcc gaggcggccc atgagggcca catccaggac gcttaccggg aaacgggaat
      481 cgaactggac atgctggggc atgtaaccga tgcggggacg ggccttcacc gggggcagac
      541 cgaagatttc gatgcggccc cggtccggtt tgaggaggcc cagcatcagt ttcagcaggg
      601 tcgtcttgcc gccggcattg ggtccgacga tggagatgaa ggtccgggcg gggatggcga
      661 actgtacccc tgccagtacc ggggcgccgt tgtaggaaaa attgacatcg gtaaatttga
      721 cgacggttcg atcattcatt tttcattgta cccacgccgg gcatggcgct cagaggccaa
      781 gaccttgttc caccgccgcg gcgatgcggt tcagattgtc caggtaatcc cgggccaggg
      841 gatcgatggg caccacgacg cccccgatcg cccgggccac ggcggcggcg ctcttggcgg
      901 gaaactggga ctgaacgaag attaccttga cgccgtctct tcgcgcggcg gcaatgaggg
      961 acgccaactg ccgggcgctc ggttcccggc cgccccgttc cacgggcgtc tggatcaggc
     1021 cgtaggcggc ggcgaaatat ccgaacgagg ggtgatagac gtacagcttc ttccccttga
     1081 gcggggcaag cgcccgggcg atccgggcgt ccacccgatc cagatccgct tggaacgcct
     1141 ggagattagc cccgtattcc tgccggtggg ccgggtccag acggcagagg gcgtcatgga
     1201 tgttccgtgc ctggatcttg accagccggg gatccagcca gatgtgcggg tccggcgccc
     1261 gccgcccccc cgctggatgt gggggcgccc ccggtttccc ggctgttttc ccgccccggg
     1321 cggaggttcg cccctgttca tgagtcgagg cgtcggtttt ctcctttacc ttccgcggct
     1381 tccctttacc gtcgttgccc ccgttgtcat ggccttcttc atcgggatac cggaagggaa
     1441 caccctgctg ggtggcgacg atctcgatct tccggtggga ggccccgatc ttacccagta
     1501 cggctctttc caccggcacg ccgatgcgga agtaggcccg ggccccgctg agtctggcca
     1561 tctgccgcgg tgtcggttca tagctgtggg gggactgccc ctcccccacc agcaccgaga
     1621 catccacgtg ccggcccccg atccgctcga cgaaataggc ctggggaaga atggtgacaa
     1681 agatggggag ccgcggcgtc ggtccggggg atgagccccc cccttcctgc ccccggagga
     1741 gcggcgccgg gaccgccagc aggacgccgg ccaggcagca gcagcaaagc aggcgcccga
     1801 acatagatcc ggaccgtttt ctattcaggc ttggcgacga cattcgcaca ttcgataaaa
     1861 agctcatggc aatcttcgca gcgcctgatg gcatggtttt gcgcgaaacc ttcccagtgc
     1921 tccttctgct ttggcgaaag gatccccgta ccgttgcaga gcggacagac attttccagg
     1981 gccgtcatga tcgccgcccg gatgaactcc gagcggttgg ggatgttgct gatcacctcc
     2041 cggaggtcct cgttgacctt
//
LOCUS       DAOSMF010000240         2050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_59161_length_2050_cov_20.781955, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000240 DAOSMF010000000
VERSION     DAOSMF010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59161_length_2050_cov_20.781955"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1644)
                     /locus_tag="PLT63_11570"
     CDS             complement(1..1644)
                     /locus_tag="PLT63_11570"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOD99402.1"
                     /translation="MTEKANTSEARQKKREREYADNRRYWLSEEEKLYARWEEAYYPG
                     GQDQVDRLIKQGKKPVRELIRQLIDPGTEFFELSRGAGFGINYESTKDVPSAGLATGL
                     GKVHGNWVMMICNDSRVKAGAYYPINTKKHLRAQDIADEAGIPCVYIADSAGGFLPMQ
                     AGVFPGRGQFGRFFFNMCRMSAKGLKQYTLSTGGNTAGGAYIVYLACESIMIDKMAYS
                     FLGGPPMVKSAIGEVVSMEELGGAKVHTGISGGADHFVRSQDEGIEKLRELLSYDPKQ
                     KLCIERRETKEPRVPITRLYEILPKDTYRGINVREVIATIADDSCLSEYKRNYNPGRG
                     DNIVCGKIYIKGIPVGVIASNNNGVIYVDAARKATEWVVRCCTQKIPLLYIQASPGYM
                     VGTQEEYAGIGKYGSDMVRACACAKVPKVQWVIGPDHGAANYGMCGRAYDPRFIFHTM
                     RARTSVMSGQTAGFILTSLERANAKKAGREMDEEAARKFQEMMVDKYSREAHPFFLEA
                     RLFQDGTLPFKDCRDALATAFEVSLLKPIEESSFGNFKF"
BASE COUNT          435 a          612 c          496 g          507 t
ORIGIN      
        1 tcagaattta aagttgccga agctcgattc ctcgatgggc ttcaaaagcg acacctcaaa
       61 agccgtggcg agggcgtcgc gacaatcttt aaaggggagc gtgccgtcct gaaagagacg
      121 cgcctccagg aaaaacggat gggcctcacg ggaatattta tccaccatca tctcctggaa
      181 tttgcgggcc gcctcttcat ccatctcccg acctgctttc ttggcgttgg ccctctccag
      241 gctcgtcagg ataaacccgg ccgtctgccc ggacatgacc gatgtccgcg cccgcatcgt
      301 atgaaatatg aaacggggat cataggcgcg cccacacata ccataattgg cggcgccgtg
      361 atcgggaccg ataacccatt ggacttttgg cactttcgcg caagcgcagg ccctgaccat
      421 atccgatccg tacttgccaa tgccggcgta ttcctcctgg gtgccgacca tataaccggg
      481 tgaggcctgg atgtagagga gaggaatttt ctgcgtgcag catcggacga cccactccgt
      541 cgcttttcgc gccgcatcca cgtaaatgac gccattgttg ttagaggcaa taacaccgac
      601 gggaattccc ttgatgtaga tcttcccgca gacaatatta tcaccccggc ccggattgta
      661 atttcgcttg tattcgctca agcaactgtc atcggcgatc gtggcgatca cttcccgcac
      721 gttgattccc cggtaggtgt ccttgggcag tatttcatag agacgcgtga tgggaacccg
      781 cggttccttt gtttcccttc tctcaatgca cagtttctgc ttcggatcat aagacagcag
      841 ttctctgagt ttctcgatcc cttcgtcctg actgcgcaca aaatgatcag ctccaccgga
      901 gatgccggta tgaaccttgg cgcccccgag ctcctccatg gaaaccacct cgccgatggc
      961 ggacttgacc atcgggggac cgccaaggaa ggaataggcc atcttgtcga tcatgatcga
     1021 ctcacaggcc agatatacta tgtatgcgcc gccggcagtg ttcccgcccg tactgagggt
     1081 gtactgtttg agccctttgg cggacattcg gcacatgtta aaaaagaagc ggccaaattg
     1141 tccccggccg gggaatacgc cggcttgcat gggcagaaat ccgcccgccg aatcggcgat
     1201 gtaaacgcac ggaatgcccg cctcgtcggc aatatcctgc gcccgcaggt gtttcttcgt
     1261 attgatcgga taataggcgc cggcctttac gcgactgtcg ttacagatca tcatgaccca
     1321 gttgccgtga acttttccca ggccggtcgc cagaccggcg ctggggacgt cttttgtgga
     1381 ctcataattg attccgaaac cggcgccccg gcttagttca aagaattccg tgccggggtc
     1441 gatcagttgc ctgattaact cacgcaccgg ttttttcccc tgtttgatca aacgatcgac
     1501 ctgatcctgg ccacctggat aatatgcctc ttcccaccgg gcataaagct tctcctcttc
     1561 cgacaaccaa tatcttcggt tatccgcgta ttccctttct ctttttttct gtctggcctc
     1621 gctggtattt gccttctccg tcatttatcc tgctcctttt ccggaatgtc cgggacgtta
     1681 tggtagctcc cgtaaggcga tcattgacac cggcaaaagc gataatgccc gcctcggtca
     1741 acgtgcttgc gggagacacg atcatttccc ctaccacaca atcatgttgc cgggccaatc
     1801 ataatcgcca cggcgcccat gtaagttgac cggatcacct ctggaaaaac agtataaccc
     1861 caccttccgg aaactatgct ataactactt gatatgattc acgatggatt ttcattttta
     1921 tggactacat cttcattttt tacgctttag tcgaatgaac cgcaattatc accctgcgct
     1981 tggccaataa ttatttccgc ccgcctcccg gagcatggag accagtttcc cgttgacacc
     2041 cccgccggca
//
LOCUS       DAOSMF010000241         2005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_60954_length_2005_cov_27.645128, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000241 DAOSMF010000000
VERSION     DAOSMF010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60954_length_2005_cov_27.645128"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="PLT63_11575"
     CDS             <1..510
                     /locus_tag="PLT63_11575"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTAP family purine nucleoside phosphorylase"
                     /protein_id="HOD99403.1"
                     /translation="ALKSLGVREVIGINSTGSLKQALPPGVLVVPDDFIMLHPGPSVH
                     STPAAHVTPDLSEEVRRKWLAAAADCGIAAVDGGVYWQTAGPRFETKAEIRLMSRAAD
                     LVGMTMAAEAIVARELEMDYASLCSVDNYAHGLVDRELTMEQVLQCARRNGDTIAGIV
                     KCYVERRNR"
     gene            507..1028
                     /locus_tag="PLT63_11580"
     CDS             507..1028
                     /locus_tag="PLT63_11580"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOD99404.1"
                     /translation="MKFSKKGDGGETSLLGGQRVPKYDPRPEAYGVLDEASSALGVAR
                     AMTDDRRIGEIILGVQKDLLTMGAELSSLPGDIPKLGVRIGAADAQRLEDLIDELQQV
                     VKLKKEFIYPGDKVVAAQIDVGRTIVRRAERKVALLKREGLVDNPHIHEYLNRLADML
                     FVLARYDEQRGKV"
     gene            complement(1025..1459)
                     /locus_tag="PLT63_11585"
     CDS             complement(1025..1459)
                     /locus_tag="PLT63_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99405.1"
                     /translation="MTRKETGSFRMKHPPDRQVAPAITEAVRNRKRNNELSCAQAERI
                     AGQQQVDLAEIGVALDLLEVRIGKCQLGLFGYEPASKAVAPAVAVAPALAAAIREALA
                     GGRLPCKAAWDIAARMKIGRKKVAAACEALRIKIKPCQLGAF"
     gene            complement(1475..1930)
                     /locus_tag="PLT63_11590"
     CDS             complement(1475..1930)
                     /locus_tag="PLT63_11590"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOD99406.1"
                     /translation="MIASQLSIFAENKPGKLAAVTEILAKGKINIRATAISTSDTFGV
                     IHLIVDDPEKALAALTKAGMLVKIRPVLAVLLDDRPGGLDRLAQLMAKEGINVNNAYG
                     FVLENRVSAVFVVDVDDPERAVKIIEKKGFKTLDAEALAAVEPFHYMKY"
BASE COUNT          369 a          618 c          632 g          386 t
ORIGIN      
        1 gccttgaaga gcctgggggt ccgggaggta atcgggatca attccacggg ttccctcaaa
       61 caggccctgc cgcccggggt cctggtcgtt cccgacgatt tcatcatgct ccatcccgga
      121 ccctcggtac attccacgcc tgccgcccac gtcacgccgg acctgagcga ggaagtacgg
      181 agaaagtggc tggccgccgc cgccgattgc gggattgccg ccgtcgatgg gggggtttac
      241 tggcagaccg cgggcccgcg ttttgaaacg aaggcggaga tcagactcat gtcccgggcc
      301 gccgatctgg tgggcatgac catggccgcc gaggccatcg tcgcccggga gctggaaatg
      361 gactatgcct ctttgtgttc cgtggacaac tatgcccatg ggctggtgga tagggaactg
      421 acgatggagc aggtccttca gtgcgcccgg aggaacggcg ataccatcgc gggaatcgtc
      481 aaatgctatg tggaaaggag gaatagatga agttttcgaa aaagggggat ggaggggaaa
      541 cgagtctcct cggcgggcag cgggtaccga aatacgatcc cagaccggag gcttacggcg
      601 tcctggacga ggcgagctcg gccctcgggg tggccagggc catgaccgat gaccggcgga
      661 ttggggagat catcctcggc gtgcagaaag acctgctgac catgggggcg gagctgtcca
      721 gcctgcccgg ggacatcccg aagctcgggg tgcgcatcgg tgccgccgac gcccagcgtc
      781 tcgaagatct catcgatgaa ctccagcagg tggtcaaact gaaaaaagaa ttcatctacc
      841 ccggggataa ggtggtcgcc gcccagatcg acgtcggccg gacgatcgtt cgccgggcgg
      901 aacgaaaggt ggccctcctc aaacgggagg gcctggtgga caatccccac atccatgagt
      961 atctgaaccg cctggccgac atgctcttcg ttctggcccg gtacgacgaa cagcggggaa
     1021 aggtttaaaa ggcccccaac tggcagggtt tgatctttat ccgcagggcc tcgcaggccg
     1081 ccgccacctt tttgcgcccg attttcatcc gggcggcgat atcccaggcg gctttacagg
     1141 ggagacggcc gcccgccagg gcttcccgga tcgccgccgc cagcgccggc gccacagcga
     1201 cggcgggcgc cacggccttg ctcgccggct cataaccgaa gagccccaac tggcacttgc
     1261 cgattcgcac ctccagcagg tccagcgcca ccccgatttc ggcgagatcc acctgctgct
     1321 gtccggcgat acgctccgcc tgggcgcagc tcaactcgtt gttccgcttg cggttccgga
     1381 cggcctccgt gatggccggc gccacctggc ggtccggcgg atgtttcatc cggaaggagc
     1441 cggtctcttt tctcgtcatc ttggtatgcc tccctcagta tttcatgtaa tggaaaggtt
     1501 ccaccgccgc cagcgcctcg gcgtccaggg tcttgaaccc cttcttttcg atgatcttga
     1561 cggcccgttc cgggtcgtcc acatccacga caaaaacggc gctgacccgg ttttccagga
     1621 cgaagccata ggcgttgttg acgttgatgc cttccttggc catgagttgg gccaacctgt
     1681 ccagaccgcc gggacggtcg tccaggagga cggcgagaac cgggcggatc ttgacgagca
     1741 tccccgcctt ggtcagggcg gccagggcct tttccggatc gtccacgatg agatggatca
     1801 ccccgaaggt atcggaggtg gaaatggccg tcgcccggat gttgatcttt cccttggcga
     1861 ggatttccgt cacggcggcg agcttgcccg gcttattctc cgcgaaaatg gataactgac
     1921 tggcgatcat atttctcctc cctggtcaat cttgacgaca tcgtaaaaag tccatatgcg
     1981 gcgttgcgct tcatccttcg tcact
//
LOCUS       DAOSMF010000242         1978 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_62032_length_1978_cov_20.078523, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000242 DAOSMF010000000
VERSION     DAOSMF010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62032_length_1978_cov_20.078523"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="PLT63_11595"
     CDS             complement(<1..687)
                     /locus_tag="PLT63_11595"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOD99407.1"
                     /translation="MFAGLERLDQTQFLLRRDPGEYRRALGLFDYLFVRKALRLPAPV
                     LNYSPPWGMRGIFDEDGIMDNLKRMFDPTIIALIGAGDQADTPGRQILDNLRTCRERK
                     IYSVNPRHACIDGEPCYPHIGAVPEHVDLAIVATPAPTVPEIVEACGRADVDGMIIIS
                     AGFREAGEKGRRLEQEILRIKKQYPLRILGPNCLGIIRPHAGLCASRLPGAPEAGQIA
                     FITQSNAFGKI"
     gene            805..1623
                     /locus_tag="PLT63_11600"
     CDS             805..1623
                     /locus_tag="PLT63_11600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOD99408.1"
                     /translation="MAAKYVKGRLYKLKIAELQADPEQPRKFMDPIALNELTASIQKF
                     GVLVPIQFRQDEAGALIIVSGHRRVQAAGRAGRTEISGTYTEGDTRLQGFVENLQREN
                     MLPIDEAEQMQSLMKEYALNQYQLAEAIGKSQPAVNASLSLNKLPEDIRDSIRTNPNI
                     TKTMLLDVVKMKTEKSMRRKFEGLMARGGQKASQAVRKEKVTKQRAFLNRTDALTSEI
                     ASLPWSEWSEDDRIDLANALRGLRQSAGALLTAMDEPRDAEEEEQERPASMNLS"
BASE COUNT          442 a          568 c          578 g          390 t
ORIGIN      
        1 gatctttccg aaggcgttgc tctgggtgat aaaggcgatc tgtcccgcct ccggggcgcc
       61 ggggaggcgg gaggcgcaga ggccggcgtg ggggcggatg atgccgaggc agttggggcc
      121 gaggatccgc agggggtatt gcttcttgat ccggagtatc tcctgctcca ggcgccggcc
      181 cttctctccg gcttcccgga acccggcgga gatgatgatc atgccgtcca catcggcccg
      241 gccgcatgcc tcgacgatct ccgggaccgt cggggcgggc gtggccacga tagccaaatc
      301 gacgtgttcc gggacggcgc cgatatgggg gtagcagggc tccccgtcga tacaggcatg
      361 gcggggattg acggagtaga tcttccgttc ccggcaggtc cggaggttgt ccaggatctg
      421 tcggcccggg gtgtccgcct ggtcaccggc tccgatcagg gcgatgatcg tggggtcgaa
      481 catgcgcttc aggttgtcca taatcccatc ctcgtcgaat attcctctca taccccaggg
      541 aggggaataa ttcaagaccg gggcgggcaa ccggagagcc tttcggacga agagataatc
      601 gaaaaggccg agagcacggc ggtattcgcc cgggtcgcgc cggagcagaa actgcgtctg
      661 gtcgaggcgc tccaggcccg caaacatttt tatacaccta tgtgcccgtg atgaatattg
      721 atttttgttc atacatcttt cttgactttt ataacgcagc gcctactatg ggttatatat
      781 tatcctcgaa aggagcaatc gcaaatggcc gccaaatatg tcaagggacg tctctataag
      841 ctgaagatcg ccgagttgca ggccgatccc gaacagccgc ggaagttcat ggacccaatc
      901 gccctcaatg aacttacggc ctcgatccag aaattcgggg tcctcgtccc catccagttt
      961 cgccaggacg aggccggggc gctgatcatc gtctctggac atcgtcgcgt ccaggccgcc
     1021 ggacgggcgg ggcggacgga aatcagcggc acctatacgg aaggcgacac gcgtcttcag
     1081 ggtttcgtgg agaatctcca gcgggaaaac atgctcccca tcgacgaggc ggaacagatg
     1141 caatccctca tgaaggaata cgccctgaac cagtaccaat tggccgaggc gatcggcaag
     1201 agccagcccg ccgtcaacgc ctccctctcc ctgaacaagc ttcccgaaga catccgcgat
     1261 tcgatccgga cgaatcccaa catcaccaag acaatgcttc ttgacgtggt gaagatgaag
     1321 acggaaaaga gcatgcgccg gaagttcgag ggattgatgg cacggggcgg ccaaaaggcc
     1381 tcccaggccg tcagaaaaga gaaagttacg aagcagcggg ccttcctcaa ccggaccgac
     1441 gccctgacca gcgagatcgc gtcattgccc tggagcgaat ggtcggaaga cgaccgcatc
     1501 gacctggcca acgccctccg gggtctccgc caatccgccg gcgccctcct gacggctatg
     1561 gacgaacccc gagatgccga ggaagaagag caggaacgtc cggcttccat gaacctctcc
     1621 tgatggaaga ctaatggaga ttgccgactc tgcggttcat aattagttga taatatatat
     1681 ttttattcca ttttcgctcc gaaagccacg ccggcccgca actgccgcat taactatata
     1741 atatcgaatg cttacctttg atcagacgct gatctttttt ggccgactgc cgatattgca
     1801 taagctgcca ggcgttttgc gcttaccgtg taattacgcg tacgccggga tcgaggcctt
     1861 gccatgattt gtttacggaa ccggtgaaag cggacaaacg cgattcatca aaaccacgga
     1921 aggacttccg taaaaagttc tcccgtctac tcaaggatgc cagcaatgat acagccat
//
LOCUS       DAOSMF010000243         1765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_72641_length_1765_cov_23.270175, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000243 DAOSMF010000000
VERSION     DAOSMF010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72641_length_1765_cov_23.270175"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1765)
                     /locus_tag="PLT63_11605"
     CDS             complement(<1..>1765)
                     /locus_tag="PLT63_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99409.1"
                     /translation="TAGMDDLDKKLAEIERDIAKMRANPNADLKLIDEIELQRQTAAI
                     KAWYDEYLQEQIKAKENFDRQEKAKQDSIAKGAQETADAIQKQYDADGQLLRDKLELY
                     KDLTGFEDEYRQVQLDWIERIKKEEIKATGDVAAAEKKAAKERAQIEYNLFKTKTDYI
                     ADGFGQLQSAFTEIAGVYEKGSDAARQWEQAAKAMEIAQRAVAVVQAVAAIATQGLGD
                     PYTAFARVAAMAATMGALLASIGEGIGTGGGPSGSSAASTTETYTGQSTVLGAADGTA
                     SESIAKSWELLQDTYDMEYRELSGIYDEMKDLNSNITGLVGNIIRYGVTSSSWASVPG
                     DTSLTDSGWLSANWLRNLGSSINDLFSNIPLIGNIVGGVLDKAIGWVSSISDWFVNGI
                     FGGSSSSHVALSGITVSGGTIKDLIAGLNSSVNKYAYIVTHTSGGWFGHDTDTGVYQY
                     AGVDEEVTSMFTKVFKNLSTTLVQLVAELGGDVQQALNYSFGSDMIELKDLSSDQINS
                     TIQSYLSRISDNAAEALLGPLLSKYQQVNEGLFETAQRVLLNKAVVMDSLEMTGQAFT
                     GTTAQVIEFSESIVDMAGDLDT"
BASE COUNT          358 a          505 c          442 g          460 t
ORIGIN      
        1 gtatccagat ccccagccat atccacgatg gattccgaaa actcgataac ctgggccgtc
       61 gtgccggtga aggcctgtcc ggtcatttcc aggctgtcca tgacgactgc cttgtttagc
      121 aggacccgct gagcggtctc gaacagcccc tcgttcacct gctggtactt gctgagcaaa
      181 ggaccaagga gggcctccgc tgcattgtcg ctaattctcg acaggtaaga ttggatcgtg
      241 ctgttgatct ggtcggagct gaggtccttc agttcgatca tgtcggagcc gaaagagtaa
      301 ttgagggcct gctgaacatc gcctccaagc tccgcaacta gctggaccag ggtggtgctc
      361 aaatttttaa acaccttggt gaacatggag gtgacctcct cgtcaacgcc ggcatactga
      421 tagaccccgg tatcggtatc gtgtccgaac cacccacccg atgtatgggt gacgatgtag
      481 gcgtatttgt tcacggatga attgagaccg gcaattagat ccttgatggt tccaccggaa
      541 actgtgatgc cggaaagcgc gacatgggac gatgacgatc cgccgaagat gccgttgaca
      601 aaccagtcgc taatagagga aacccatccg atagccttgt cgagcacccc cccgacaata
      661 tttccaatca aagggatgtt tgaaaaaaga tcgtttatgc tcgacccaag gtttcggagc
      721 cagttcgcgc ttaaccaccc gctatcagtc agcgaggtgt ctcccggcac actggcccaa
      781 ctggaggacg tgaccccata ccggataatg ttgccaacca gcccggtgat attgctattc
      841 aggtccttca tttcgtcata aatacccgaa agctcccggt attccatgtc gtaggtgtct
      901 tgcaggagtt cccacgactt ggcgatggat tcactggctg ttccatccgc agcgcccaaa
      961 acggtgcttt gccctgtgta ggtttcggta gttgaggctg cggaagaacc cgaggggccg
     1021 ccgcctgtgc cgatcccttc cccgatagaa gccagcaacg cccccatcgt ggccgccatt
     1081 gcagctacgc gggcaaaagc tgtgtagggg tcgcccaagc cttgtgtcgc aatggccgcg
     1141 accgcctgaa cgactgcaac ggccctctgt gcaatttcca tcgcctttgc cgcttgttcc
     1201 cattgcctgg ccgcatcaga acccttttca taaacccctg ctatttccgt aaaggccgat
     1261 tgaagctgcc cgaatccgtc tgcaatataa tcggtctttg ttttgaaaag gttgtattcg
     1321 atttgagcgc gttccttggc tgctttcttt tcagccgccg ccacgtcgcc cgtcgctttt
     1381 atctcttctt tcttgatcct ctcaatccaa tcaagttgaa cctgtctgta ttcatcctca
     1441 aagcccgtca ggtctttgta aagttcaagt ttatcgcgga gtaattgccc gtccgcatcg
     1501 tactgctttt ggattgcgtc ggcggtttcc tgtgcgcctt tggcgatgga atcttgtttg
     1561 gccttttcct gccggtcaaa gttctctttg gctttaatct gctcttgcag gtattcgtca
     1621 taccacgcct tgattgctgc cgtttgccgc tgcaattcga tttcatcaat caacttcaaa
     1681 tcggcgttag gattggcccg catctttgca atgtcgcgct cgatttcagc cagcttcttg
     1741 tccaggtcat ccatgcccgc agtgg
//
LOCUS       DAOSMF010000244         1753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_73369_length_1753_cov_17.375736, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000244 DAOSMF010000000
VERSION     DAOSMF010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73369_length_1753_cov_17.375736"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="PLT63_11610"
     CDS             complement(<1..509)
                     /locus_tag="PLT63_11610"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOD99410.1"
                     /translation="MNISALWIRRPIMTSLVMIGILFFGIIGYRNLPVNDLPQMDYPT
                     IQVSAALPGASPEIMAATVATPLEKKFSTIQGLESMTSTSTQGSTSITMQFSLDRSID
                     AAAQDVNSMISAAMGILPTMPAPPTYQKVNPAEWPIMFYAMVGETIKDTTTYEYVDSV
                     IAPNLSTIKG"
     gene            complement(506..>1753)
                     /locus_tag="PLT63_11615"
     CDS             complement(506..>1753)
                     /locus_tag="PLT63_11615"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOD99411.1"
                     /translation="VKYLMFDKIILATGHGGRQHRNGSWRSWLRRLISGAFLLSLVAA
                     VALVAGACGKESPPPAFPPATVLAAKVVKKDMPLAIAAIGTVEAHKSINVYSRVTGQL
                     LRTHFREGEDVRAGQLLFTIDPAPFREKLHQFEARLAKSIAQSQFNEEEAKRYAFLLS
                     KGAVSKSEADKYLTDAAAYSATVKADRAELEDARLNLSYCSVKAPFAGRTGAYGVNLG
                     AIVRANDTSLTTLNQISPVYVRFSVPEKHLGDIRKYLAAGTVSVRVIPDKDFPREIPY
                     GKLVFVDNAIDQATGMIKLKAEFTNNDRALWPGQFVNVSLVLTIQAGAVVAPLRAVQT
                     GDQGRFVFVIKPDMTAEVAPVVVDRSAGEEAVIARGLQGGETVVADGHLKVQPGAKVD
                     IRDGLGAGPGNGNAKGNAGGGGR"
BASE COUNT          337 a          500 c          555 g          361 t
ORIGIN      
        1 cccttgatgg tggaaaggtt cggggcaatg accgaatcca cgtattcata ggttgtcgtg
       61 tcctttatgg tctcccccac catggcgtag aacatgatgg gccattcggc gggattgacc
      121 ttctggtacg tagggggagc cggcatggtg gggaggattc ccatggccgc cgagatcatg
      181 gagtttacat cctgggccgc cgcatcgatg ctgcggtcca gggaaaactg catggtgatg
      241 gaggtcgatc cctgggtgct cgtggaagtc atggattcga gcccctgaat ggtggagaat
      301 ttcttttcca gcggggtggc caccgtggcg gccatgatct ccgggctggc gccgggaagc
      361 gccgccgata cctggatagt gggataatcc atctggggca ggtcattgac gggaagattg
      421 cgataaccga tgattccgaa aaacagaatg ccgatcatca ccaggctggt catgatgggt
      481 cggcggatcc agagggcgga gatattcacc tgcctccccc tccggcgtta cccttggcgt
      541 tgccgtttcc cggcccggcg ccaaggccgt cccggatatc cacctttgcc cccggctgca
      601 ccttgaggtg tccgtcggcc accaccgttt ccccgccctg gagacccctg gcgatcaccg
      661 cctcctcgcc ggcgctccta tccacgacca caggggcgac ctcggccgtc atatccggtt
      721 tgatcacaaa gacgaaccgc ccctgatcgc cggtctggac agcgcggagg ggcgcgacga
      781 cggcgccggc ctggatcgtg aggacgagag agacatttac aaactgaccc ggccaaaggg
      841 cccggtcgtt attggtgaat tccgccttca atttgatcat ccccgtggcc tggtcgatgg
      901 cattgtccac aaagacgagt tttccgtagg gaatttccct gggaaagtcc ttgtcgggga
      961 taacccgcac ggaaaccgtt cccgccgcaa gatattttct gatgtctccc aggtgttttt
     1021 ccggtacgga gaaacggaca tagacgggac tgatctggtt gagggtggtc agggaggtgt
     1081 cattggcccg gacgatggcg ccgagattga cgccgtaggc gccggtacga ccggcgaaag
     1141 gggccttgac ggaacagtag gaaagattga gccgggcgtc ttccagctcg gcgcgatccg
     1201 ccttgacggt ggcgctgtaa gcggcggcgt cggtcaggta cttgtccgcc tccgattttg
     1261 ataccgcccc cttggataat agaaaggcat accgtttcgc ttcttcttca ttgaactggg
     1321 attgggcgat gcttttggct agacgggcct cgaactgatg aagcttttcc cggaaggggg
     1381 ccggatcgat ggtgaagagc agttgtcccg cccggacatc ctctccttcc cggaaatggg
     1441 tccggagaag ctgcccggtc acccgggaat agacgttgat ggatttatgg gcctccacgg
     1501 tgccgatggc ggcaatcgcc aggggcatat ccttcttgac gaccttggca gccaggacgg
     1561 tggcgggagg gaaagcgggc ggcggggact ctttgccgca ggcgccggct accagcgcta
     1621 ccgcggccac taaggaaagc agaaaggctc cgctgatcaa acgccgtaac cacgatcgcc
     1681 acgaaccatt ccggtgctgc cggccgccat gccccgttgc caatatgatc ttgtcgaaca
     1741 taagatattt gac
//
LOCUS       DAOSMF010000245         1700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_76627_length_1700_cov_19.255927, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000245 DAOSMF010000000
VERSION     DAOSMF010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76627_length_1700_cov_19.255927"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="PLT63_11620"
     CDS             <1..500
                     /locus_tag="PLT63_11620"
                     /inference="COORDINATES: protein motif:HMM:NF012783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HOD99412.1"
                     /translation="VQAHKNAMYMVHDPWVLAAGNQYDLREIADILGKIGGNMLDIYY
                     DKSNIGKRELKQMMKDETWFTAAEAKDRGLIDTILDTGAAKAKFDLSIYANVPDELED
                     SDREGATLSKQEIERALRDAGASRSFAKSIAARGSNGNSQRDVGGVKADIDKILKLQQ
                     IIGGK"
     gene            503..>1700
                     /locus_tag="PLT63_11625"
     CDS             503..>1700
                     /locus_tag="PLT63_11625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HOD99413.1"
                     /translation="MDINQVIEDLGRSFEAFKAENDKRLKEIETKGGADPLLTEKVEK
                     INAEISQIAALKKQIEHVETVAGRGGFGGGHSGLDQAKAEYKAGFEKWFRKGIEGNLA
                     QLAVQASASTLDDTAGGFLVPEEMAATIDRIAGVTSAMRRLASVMSIGTDTYKKLVNQ
                     GGANSGWVGEKSARVETATPILAEIAINTKEIYAMPAATQTLLDDSSVDIAAWLGNEV
                     AIEFAEEEGQAFIKGNGVDKPKGLEAYSTVANASYAWGKIGYIASGAASTFTNADKLF
                     DLQHALKPIYRNGAAFLMNDNTLLNIRKFKDGEGNYLWRPGLLEGAPDTLLGKPVEID
                     DNVADIGQNTYPIYFANFKRAYLIIDRLGIRVLRDPYSSKPYILFYTTKRVGGGIVMY
                     EAIKTLK"
BASE COUNT          469 a          389 c          501 g          341 t
ORIGIN      
        1 aagtgcaggc gcataagaat gccatgtaca tggtacatga cccctgggtg ctggcggctg
       61 ggaatcagta cgacctccgg gaaatagctg acatcctcgg caaaatcggt gggaacatgt
      121 tggatattta ctatgacaaa tcgaacatcg ggaagcgtga attaaagcag atgatgaaag
      181 acgagacttg gttcacggcg gcggaggcga aagacagggg attgatcgac acgatcctcg
      241 ataccggcgc ggcaaaggca aagtttgact tgtcgattta tgcgaatgtc ccagatgagc
      301 ttgaagattc cgaccgggaa ggagctacat tgagtaaaca agagattgag cgtgctctgc
      361 gtgatgcagg tgcaagccgg tctttcgcga agtccatagc tgcgagaggc agtaatggca
      421 actcccagcg cgatgttggg ggcgtaaagg cagatattga caagatacta aaactacaac
      481 agattatagg aggtaagtaa agatggacat taatcaagta atagaagacc tggggcgatc
      541 attcgaggcg ttcaaggcgg agaacgacaa gcgcctcaag gaaatcgaga caaagggcgg
      601 agccgatcct ctcttgacgg aaaaagtcga aaagatcaac gcggaaatct cgcagatcgc
      661 ggctctgaaa aagcagatcg aacacgtaga aacagtggcc ggacgcggcg gttttggcgg
      721 aggacattcg gggctggatc aggcgaaggc cgaatacaag gccggttttg aaaagtggtt
      781 ccgcaaaggg attgaaggca atctcgcaca acttgcagtg caggcttccg cttctactct
      841 tgacgacacg gcgggtgggt tcctggtacc ggaagagatg gcggcgacca ttgaccgaat
      901 tgccggagtt acttcggcca tgaggcggct ggcctccgtg atgagcattg gaacagacac
      961 gtacaagaaa ctcgtcaatc agggtggcgc caattccggt tgggtcggcg aaaaaagcgc
     1021 acgggtcgaa accgctacac cgatactggc ggaaatcgct atcaatacga aagagattta
     1081 tgcaatgccc gcagcgacgc agacactgct cgatgactcc agcgtggata tagccgcgtg
     1141 gctcggcaat gaagtcgcaa ttgagttcgc tgaagaggaa gggcaggcat tcatcaaagg
     1201 aaacggcgtc gacaagccga agggacttga ggcttattcc actgttgcca atgcctctta
     1261 tgcgtggggc aagattggtt acattgcatc cggcgccgcg tccacattca ccaatgcaga
     1321 caagttgttc gaccttcagc acgcattgaa gccgatctat cgcaacgggg cagcgttcct
     1381 catgaacgac aacaccctgc ttaatatcag gaaattcaag gatggtgaag ggaattacct
     1441 gtggaggccg ggcttgctgg aaggcgcgcc tgacaccctg ctcggcaagc cggtcgagat
     1501 cgacgacaac gtagccgata tcgggcagaa tacgtatccg atctattttg ccaatttcaa
     1561 gcgggcatat ttgatcattg accgtttagg aattcgggtg ctccgcgatc cttattcctc
     1621 caagccgtat attctcttct acactacaaa gagagtcggc ggcgggatcg tgatgtatga
     1681 agcaattaag acgttgaaag
//
LOCUS       DAOSMF010000246         1649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_79990_length_1649_cov_25.692597, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000246 DAOSMF010000000
VERSION     DAOSMF010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79990_length_1649_cov_25.692597"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /gene="lgt"
                     /locus_tag="PLT63_11630"
     CDS             complement(<1..752)
                     /gene="lgt"
                     /locus_tag="PLT63_11630"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOD99414.1"
                     /translation="MEELLFFWQHLPGRLSPVLLEFGSFQVRYYSLMYLIAFTLTYLL
                     AGYRIRDEKHPYDMETIQDIMIWMILGLILGGRLGYVLFYNCDYYLQRPWEVFLPFDF
                     SQGIRFVGISGMSFHGGALGALAAAIIFCRRRGLDFWRLADLFCPAIPLGYTFGRIGN
                     FINGELYGRPTEVPWGMYFPLDQEGLLRHPSQLYEAFGEGILLFALLWSLRKRSPVDG
                     FLLATYLMGYGLVRFFIEFYRQPDAQIGYLWG"
     gene            complement(753..>1649)
                     /gene="trxB"
                     /locus_tag="PLT63_11635"
     CDS             complement(753..>1649)
                     /gene="trxB"
                     /locus_tag="PLT63_11635"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOD99415.1"
                     /translation="TAGLYAARAGLASLLFAGASTVSQITVTDLIENYPGLPDGINGF
                     ELMERFRQQAVKFGLEVAAADVEKLEKLTDGGKTLWMVKAGDMAGHARSVIMATGAYW
                     RKLGVPGEEDFAGRGVSYCATCDGPFYRNREVVVVGGGNTAVQEAIFLTHFAAKVTII
                     HRRNRFRAAGILAQRALANPKIQAVWNSVVEEIGGEKGVEWIRIKDVRNGAMQQIPAA
                     GVFIFVGLIPQTDLVRDLAAREKDGAIIVDREMRTSAPGLFACGDCIDKSLRQVVTAC
                     GDGATAAYAAQLYVEDYKGEAY"
BASE COUNT          356 a          491 c          511 g          291 t
ORIGIN      
        1 aaaccccaca ggtatccgat ctgggcgtcg ggctgccggt agaactcgat gaagaaacgc
       61 accagaccgt agcccatgag ataggtggcg agaaggaatc cgtcgaccgg gcttcttttt
      121 ctcaaggacc acaacaaggc gaacagaagg ataccctccc caaaggcctc gtatagttgg
      181 gagggatggc gcaacaatcc ttcctggtcc agggggaaat acatccccca ggggacctcg
      241 gtgggacgtc cgtagagctc gccgttgatg aagttgccga tgcggccgaa ggtgtatccc
      301 agggggatgg cagggcaaaa gagatccgcc aggcgccaga agtccagacc gcggcgtcgg
      361 cagaagatta tggccgcggc cagtgccccc agcgccccgc catggaagga catgccgctg
      421 atgcccacga aacggatgcc ttgggagaaa tcgaacggga ggaagacctc ccaggggcgc
      481 tggagataat agtcgcaatt gtagaacaag acataaccga ggcggccccc gaggatcaga
      541 cccagaatca tccagatcat gatgtcctgg attgtttcca tatcataggg atgtttttcg
      601 tccctgatcc ggtagccggc gagcaggtag gtaagggtga aggcgatgag atacatgagg
      661 ctgtaataac gcacctgaaa agagccgaac tccaggagca cggggctcag gcgtccgggg
      721 aggtgctgcc agaagaaaag cagttcttcc actcagtagg cctcgccctt gtaatcctcg
      781 acatacaact gggcggcata ggcggccgtc gccccgtcgc cgcaggccgt gacgacctgg
      841 cggagtgact tgtcgataca gtccccgcag gcaaagagtc cgggagcgga agtgcgcatc
      901 tcccggtcca cgatgatggc gccgtccttt tctctcgctg ccaggtccct gaccagatcc
      961 gtctggggta tgagacccac gaagatgaag accccggctg cgggaatctg ctgcatcgcg
     1021 ccgttccgga catccttgat cctgatccat tccacgcctt tctcgccgcc gatctcttcc
     1081 accacgctgt tccaaaccgc ttggatctta gggtttgcca aggcccgctg ggcaaggatg
     1141 ccggcggccc ggaagcggtt gcggcgatgg atgatggtca ctttggcggc gaaatgggtg
     1201 aggaaaatgg cttcctgaac ggcggtgttc ccgccgccca cgacgaccac ctcacgattc
     1261 cggtaaaagg gcccgtcaca ggtggcacag taagaaaccc cccgaccggc gaagtcctcc
     1321 tctccgggaa cgccgagttt acgccaatag gccccggtgg ccatgatgac ggaccgggcg
     1381 tgaccggcca tgtcgccggc cttgaccatc cagagcgtct ttcccccgtc ggtgagcttt
     1441 tccagcttct ccacgtcggc ggcggctact tccaggccga acttcacggc ttgctgccgg
     1501 aaacgttcca tgagctcgaa accattgatc ccatccggga gcccggggta attttctatc
     1561 aggtccgtaa cggtgatctg acttaccgtc gaggccccgg cgaagaggag cgaggcgaga
     1621 ccggcgcggg cggcgtagag accggcggt
//
LOCUS       DAOSMF010000247         1596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_1604
            NODE_83775_length_1596_cov_25.022713, whole genome shotgun
            sequence.
ACCESSION   DAOSMF010000247 DAOSMF010000000
VERSION     DAOSMF010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863133
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:41:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,328
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83775_length_1596_cov_25.022713"
                     /isolate="AMR_MDS_1604"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1596
                     /locus_tag="PLT63_11640"
     CDS             <1..>1596
                     /locus_tag="PLT63_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD99416.1"
                     /translation="VFYNLFNYAGHTGQYYLIVDQEGEIVYHPDRKMLGTKADADILA
                     MTKAERGVFTTRVQGKPFYVIHDSSGDRNWLLLSFVPVRAIEDKIINIRNNSLIILVI
                     CLLLAILAALIISRTLVRPIQELTDLFRQLREGAVNLQTRLKVKTRDEIGQLISWFNS
                     YLESLEEKKLVEERLQQSLTELQRTMKEAEAANRAKSDFLASMSHEIRTPMNAIIGMA
                     DILADTPLTKEQRRYVKIFRSAGENLLTVINDILDLSKVEAGQLELESIPFRLDELIE
                     TTEAVLAFRAQEKGLELKHRLEGDIPLSLRGDPVRLRQILVNLIGNAVKFTETGGVTV
                     EVARLGKAAPDVEPGRVCFRFAVMDTGIGIPSDKIGAIFDNFTQADSSVTRRYGGTGL
                     GLSISRRLVNLMGGRIWVESEPGRGSSFYFTALLDIAPEADMGSPPGAATRPAGVPGT
                     GAVAGAVVPSAAAVAGTGAGEEEAPSLRILLAEDSEDNRLLIKTYLKQSPHCLEMAEN
                     GEIAVEKFQQGEYDLVLMDMNMPV"
BASE COUNT          335 a          498 c          483 g          280 t
ORIGIN      
        1 cgtgttctac aatttattca actatgccgg tcataccggg cagtattacc tcatcgtgga
       61 ccaggagggg gagatcgtct atcatcccga ccgcaagatg ctgggaacga aggccgacgc
      121 ggacattctc gccatgacca aggcggagcg gggcgtgttc accacccggg ttcaggggaa
      181 acccttctac gtgatccatg acagctccgg cgaccggaac tggctgctgt tgagtttcgt
      241 ccccgtgcgc gccatcgagg acaagatcat caatatccgt aacaactcat tgataatttt
      301 ggtgatctgc ctgctgctcg ccatcctggc ggccctgatt atctcccgca cccttgtccg
      361 tccgatccag gagctgaccg acctctttcg gcagctccgg gaaggggcgg tcaacctcca
      421 aacgcgcctg aaggtaaaga cccgggacga aatcggccag ctcatctcct ggttcaattc
      481 ctacctggaa agtctggaag agaaaaagct ggtggaggaa cggctgcagc aatcgctgac
      541 ggaattgcag cgcaccatga aggaagccga ggcggccaac cgagcgaaaa gcgatttcct
      601 ggccagcatg agccacgaga tccgtacccc gatgaatgcc atcatcggca tggccgatat
      661 cctggcggac acccccctga ccaaggagca aaggcggtat gtcaagatct tccgctccgc
      721 cggcgaaaat ctcctgacgg tgatcaacga catcctggac ctttccaagg tcgaggcggg
      781 gcaactggag ctggaaagca tccccttccg gctggacgaa ctgatcgaaa ccacggaagc
      841 ggtcctggcc ttccgggccc aggaaaaggg cctggagctg aaacaccgcc tggaaggcga
      901 cattcccctt tccttgcgcg gcgatccagt gcgcctgcgg cagatcctgg tgaatctcat
      961 cggcaacgcc gtgaagttca ccgaaaccgg cggcgtcacc gtcgaggtgg cgcgcttagg
     1021 caaggccgcg cctgatgtgg aaccggggcg cgtttgcttc cggtttgccg tcatggacac
     1081 gggcatcggc atccccagcg acaagatcgg cgccatcttc gacaacttca cccaggccga
     1141 ttcctccgtg acaaggcgct acggcggcac cggcctgggt ctgagcatct cccggcgcct
     1201 ggtgaacctc atgggcggaa ggatctgggt ggaatcggaa ccgggacggg gcagcagctt
     1261 ttatttcacc gccctgctgg acatagcacc ggaggcggac atgggatcgc cccccggggc
     1321 ggcgaccaga cccgccggag taccgggcac cggcgccgtt gccggtgcgg ttgtcccctc
     1381 cgccgccgct gttgccggca ccggcgcggg cgaagaggag gccccttccc tgcggatcct
     1441 cctggccgag gattcggagg acaaccgcct cttgatcaag acctatctca agcagagccc
     1501 ccactgcctg gagatggcgg aaaacggcga gatcgccgtg gaaaaattcc aacaggggga
     1561 atacgacctg gtcctcatgg acatgaacat gccggt
//
